LOCUS       DAOMJU010000001        64596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_316_length_64596_cov_22.173223, whole genome shotgun sequence.
ACCESSION   DAOMJU010000001 DAOMJU010000000
VERSION     DAOMJU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 64596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64596
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_316_length_64596_cov_22.173223"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PLO58_00005"
     CDS             complement(<1..1030)
                     /locus_tag="PLO58_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73204.1"
                     /translation="MKANALTQIILMTAALIISSCYTQFSAPIVRQETYQQSVTDTTY
                     QEQETYENYTFDSSYLNDYRLGYRDGYWDSMNSQLWLGFNIYSPFYGGYRYHSPYLGY
                     YDPWFYDPFYDWGFYGYFSFYGGYYPYYGYYPHYGYYGYYYGGYPYHRHHWHGYRDGT
                     YYNEGRDELKADYVPKSTPSGSRGSSNDAGSQSGASYLPTSTSAITTTSVSGEKTTVT
                     TTNSGSTKARYSPKTTGAGNSSQEKAVVGFQTTLVPTSAPKTTVITTNTNSRGSTRST
                     ASQKSSSPRATVRKGTSSSSGSSNTGNSSSSSSSSSGSSYQPASSSSSSSSSSSSSSS
                     SSSSSSGSR"
     gene            complement(1200..2933)
                     /locus_tag="PLO58_00010"
     CDS             complement(1200..2933)
                     /locus_tag="PLO58_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73205.1"
                     /translation="MKTVKLFILSLLALTFLTCERQKANDEPVFIFQSPKVLFKNLIA
                     NNLIVPRFSPDGLDLIFNGRIDGDLWDGIYQIPIVGGLPQKIYSTTDDILYPSYSADQ
                     RQVIFSQGLARQIYLLDIVTKNVTPLPIYGNYPTILPDGSTILYTGGLDGNIRLYDLR
                     ENRHRVLTKSHSGINIFPMMTQDRVGFIWLENRKPEQVRVNQTDFDALKTKILQIFAE
                     PLLSWTISPSGEWAIASRPNGEPFGFKLYDTAFASISIQPDTLVKNVKHLAFSVDWSP
                     TGRNVVYVGNTVPQFSRENPFTYRGIFKGDLVIANLRWENIEEAELLKSPPVSNWTIF
                     PYSEPNIQGSPRFVPTNINNPPVITSVPVETARQGELYFYRVQAVEIDLFDKITYSVV
                     SGPENATILKNTGILVWQPIDSGRFEFTVAVQDNYDGIDSQTFYVDVLPAKKGDDIFY
                     QSQPVDKKASGFAASLSFLDSNGDGFLMPGEEAALQIDLKPLYAEKLDSVRLQLLCSA
                     ESREVSYDRELIFKDCQPNQWNQMIVPIKALPKLQNRSIVFWGIIETKYGFQILPASL
                     IINGKNPEKNI"
     gene            3248..4696
                     /locus_tag="PLO58_00015"
     CDS             3248..4696
                     /locus_tag="PLO58_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQO73206.1"
                     /translation="MYIILVKRSFFKKIGRLCWFSTGWIGARYIYRELIFPFLFSIGV
                     VTFILLANFLIKSVDRLLGKGLPFGVIFEFLYLNLAWIVAMSVPMAVLVATLMAYGRL
                     AEDNEITAMRSSGISFTTILVPGFIFGLVVSVVMLYFHDGVLPEFNHRARLLTSDIYR
                     KRPDLNIEPGYFIDDLPDYSIYVKEKVGESLRKVTIYNKDARDIQTTIYADSGYIGVE
                     GNTILFTLFSGQIHEIDLKGLSDYRRIDFERHRIAIPVDNLMLERRESERRGDREMTI
                     KMMRTEVERYSREYQQVIAKINNLVRKELGDTLTSFDLKKIEKKVQTLQAENLKNLPP
                     AEAARRNQKLTIFLNRIRSESNLAFNYKKQIAKYNVEIQKKYSIPFACIVFVLLGAPL
                     GVITRRGGITVSAVLSIFFFLLYYVFLIGGEELADAAIISPFWAMWTPNLILLIVGIY
                     LIYYTSSEQRVIDFSRFPYLFRRLNKKIKKNP"
     gene            4716..6053
                     /locus_tag="PLO58_00020"
     CDS             4716..6053
                     /locus_tag="PLO58_00020"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HQO73207.1"
                     /translation="MPSVKQMKRTKNFIMVDLIEKKKAGLEHSKEEIQYLIASYTKGK
                     IPDYQMAAWLMAVNFQGMTDAETFALVETMLLSGTKLDLSHIKHCKVDKHSTGGVGDK
                     VSLILAPLAVAAGCVVPMISGRGLGHTGGTLDKLESIPGFNVNLSSQEFVRLVEKYGL
                     AMGGQTEELVPADRKIYALRDVTATVNSIPLICGSILSKKIAEGIDGLVLDVKTGNGA
                     FLADYNQSQQLAKQLKRVGENFGLQIKAVITDMNQPLGRAVGNWLEVVESIATIKGQG
                     PEDLTTITLDLTAWMVVLSGIEKNFGEARELLKQLIISGEAYQKFLLMVEAQGGDISY
                     IEHPEKYKQAAWQAPVLATESGWVKNIDTKRIGMCAVALGAGRQSLDSVIKPEVGFII
                     NKKIGDYVEVGEPLVTVHAGSRRAFQNVQSAIRKAYHFSKAPVTPPPLFYETF"
     gene            6155..6841
                     /locus_tag="PLO58_00025"
     CDS             6155..6841
                     /locus_tag="PLO58_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HQO73208.1"
                     /translation="MNYPKSKRSSGILIAAEGLDGSGKSTQLYLVKRWLELSGHHVYL
                     TEWSSSDMVRQAIKRGKKKNLLTPTTFSLVHAVDFADRYHRQIQPLLKTGFITLSDRY
                     IFTAFARDAVRGCSSAWLRNLYSFAKIPDITFYFKLSPQIAAERILSNRPKIKYYEAG
                     MDLNLHPEVYDSFRIYQGKIGTEYDQMSQEFGFVEIDATQPIKTQQEKIRAIIAEKID
                     LASYKKGELE"
     gene            6838..7518
                     /locus_tag="PLO58_00030"
     CDS             6838..7518
                     /locus_tag="PLO58_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HQO73209.1"
                     /translation="MKSFYLEPLPKCDISRLSGKLIVLEGSDGSGRTTQITRIKSWLE
                     GKGYAVVNCELARSQLVGPELELARRGNTLCPTTLSLFYATDFMDQLENVIIPALRAG
                     FIVLADRYFYTLMARDLVRGTDLKWVQNIYGVAVVPDLVFYLNVSPHRLIERRWQKAN
                     AMDYWESGMDMGLSRDWAVSFLIYQQYLRKVFLKLQDMYGFIVINGNKSITAITNDIV
                     KQIEKVLI"
     assembly_gap    7716..7815
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7888..8742
                     /gene="hisG"
                     /locus_tag="PLO58_00035"
     CDS             7888..8742
                     /gene="hisG"
                     /locus_tag="PLO58_00035"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQO73210.1"
                     /translation="MITLAIQKTGRLNEHTTKLLSECGLFVSNGSTARLMTEASNFPM
                     RILFLRDDDIPECVADGLADLGIVGENVYHEKARPLKIVERLGFAKCRLSIAVPKDFS
                     YEKLTDLNGLSIATSYPNILKRFLNENNLKADIHEISGSVEIAPGIGLASAIFDIVST
                     GSTLLTNGLKEVVTVLKSEAVLVAAENLSPEKTDLLEQLLLRIRAVQAAHTRKYILLN
                     APDSAIAKICAILPGMKSPTIMPLAEPGWSSLHSVIEEHQFWERISALKAAGAQGILV
                     IPIEKMIM"
     gene            8752..10053
                     /gene="hisD"
                     /locus_tag="PLO58_00040"
     CDS             8752..10053
                     /gene="hisD"
                     /locus_tag="PLO58_00040"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQO73211.1"
                     /translation="MKVIKYPQKTSWPELVRRPAIDNKILRNLVIEIIEAVKTDGDDA
                     LRQFSWKYDNVRLKEIEILRDELASVPNEVDDDLRKAIEIARANITKFHSSQFEGIEK
                     VETTPGVICWRKSVPIESVGLYIPGGSAPLFSTVLMLGIPARLAGCPEIILCTPPNKD
                     GRIHPVILYTAQILGIERIFKIGGAQAIAAMAYGTQSVPRVAKIFGPGNQYVTIAKQI
                     VSAQGVAIDMPAGPSEVAVVADRFANPDFVAADLLSQAEHGADSQVICVTDNEELIPQ
                     VITAVKQQLAELPRRTIIEKSLESSRLILVNNLDEALEFINCYAPEHLILQTQNPEQW
                     ADKVINAGSVFLGDFTPESAGDYASGTNHTLPTNGYARNYSGISVDSFVKKITFQQIS
                     PAGLKAIAPAIDCMATTEKLQAHRNAVRIRLEAIEKGAGRG"
     gene            10028..11092
                     /gene="hisC"
                     /locus_tag="PLO58_00045"
     CDS             10028..11092
                     /gene="hisC"
                     /locus_tag="PLO58_00045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQO73212.1"
                     /translation="MKKELDVVDLEKLARRNIINLQPYATARDDYSGAIGIFLDANEN
                     SLGSVIPQKFNRYPDPHQKRLKQEIAALKNVNPEQVFLGNGSDEVIDLLIRVFCQPGT
                     EKIMILLPTYGMYAVSAASNDVGVVSVALTPNFQINLPQIFEQIAGVKLIFLCSPNNP
                     TGNCLNPADVVNLLEKFKGIVIIDEAYIDFAPQKSFLPRLKEFNNLVIMQTFSKAWGL
                     ANLRLGMAFADPQIIDWLSRLKYPYNVNGLTQELALSALERSAEKDRMVTELRTERER
                     LADSLKELPIVEKVYPSDANFLLVKFRDARSVFQYLINQKIIVRDRSQAWHCENCLRI
                     TIGTPNENDILIAKLKDFAS"
     gene            11089..12180
                     /gene="hisB"
                     /locus_tag="PLO58_00050"
     CDS             11089..12180
                     /gene="hisB"
                     /locus_tag="PLO58_00050"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HQO73213.1"
                     /translation="MKKVLFIDRDGTLIIEPPDEQVDSLEKLEFLPGAITNMNKIATQ
                     FDYQLVMVTNQDGLGTDLFPEKDFWPAHNKMLQILAGEGVVFAEILIDRSLPNDNAPS
                     RKPGIGMLTDYLKGDYDLENSFVIGDRETDVQLARNLGTKAIYLGSNESISADYKAKN
                     WSEIYNYLSNISRTATVTRKTNETDIRLVINLNGKGANRIDTGIGFLDHMLNLFATHS
                     GCDLDLTARGDLAVDEHHTVEDVALVLGEGLLKALGDKKGIERYGFLLPMDESLAQVA
                     LDFSGRREFVWECDFKREYIGKMPTELFRHFFKSFADSAACTLHIKATGENEHHKIEA
                     VFKAVGRAVKMAIRRDPTCDQIPSSKGKL"
     gene            12177..12758
                     /gene="hisH"
                     /locus_tag="PLO58_00055"
     CDS             12177..12758
                     /gene="hisH"
                     /locus_tag="PLO58_00055"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQO73214.1"
                     /translation="MKVVIIGYNSGNIHSVRLALQRLNVKAVVSSDPAVIQAADKVIF
                     PGVGEASSAMESIKANGIFEILPRLQKPTLGICLGLQLMCRDSEENATQCFGIFDIAV
                     RKFPPLAKVPQVGWNTLFDLKGDLFRDVAENSYVYFVHSYYAELSEHTVAQTEYIIPY
                     SAAMQKDNFFAVQFHPEKSGKVGEQILRNFIKL"
     gene            12755..13477
                     /gene="hisA"
                     /locus_tag="PLO58_00060"
     CDS             12755..13477
                     /gene="hisA"
                     /locus_tag="PLO58_00060"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HQO73215.1"
                     /translation="MITIIPAIDIINGKCVRLSRGDFNTQKIYNENPLEIARQFADAG
                     IRRLHLVDLDGARQKRVVNWEVLEKIATGTPLQIDFSGGIRSETDVRIAFESGARQIT
                     AGSIAISAPDLVEEWLKKFGGEKIILGADVIDRKIAIHGWQEKAPLEIMEFLQNFQEK
                     GVQQAICTEVARDGMLTGPAFELYREIKQKFPDLYLIASGGISRIDDIYQLQDDGIDG
                     VIIGKALYEGYLKLSELKGFLC"
     gene            13471..14226
                     /gene="hisF"
                     /locus_tag="PLO58_00065"
     CDS             13471..14226
                     /gene="hisF"
                     /locus_tag="PLO58_00065"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQO73216.1"
                     /translation="MLAKRIIPCLDVKDGQTVKGVNFINIRRAGDPVELGAAYAEQGA
                     DELVFLDITASIEKRGTFIELVREIAKHLNIPFTVGGGVRNADDVARLLDAGADKISI
                     NTAAVNHPEMIEKLAGRFGSQCIVIAIDTKHTENGDFVYINGGRTPTNQTTQAWARQV
                     VELGAGEILLTSMDHDGTKRGFALEITRELAENLPIPVIASGGAGTMEHFWEVFKIGK
                     ADAALAASVFHFGEIPIPELKKFLKSNGIEVRL"
     gene            14223..14819
                     /gene="hisIE"
                     /locus_tag="PLO58_00070"
     CDS             14223..14819
                     /gene="hisIE"
                     /locus_tag="PLO58_00070"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HQO73217.1"
                     /translation="MNIDFTKNNGLLPAIIQDEKTGKVLMLGYMNRESYEKTKATGRV
                     TFFSRSRQALWTKGETSGNFLEVKEILSDCDGDTLLIKVKPTGPTCHTGADTCFNEKN
                     IAEKPFIFELEEIIRDRRNNPKPGSYTNKLFDAGDERIAQKVGEEATEVVIDAVTGNE
                     MRLPEEVADLLYHLLVLLVAKDVALDDIMNVLEKRHRK"
     gene            complement(14993..17002)
                     /gene="ligA"
                     /locus_tag="PLO58_00075"
     CDS             complement(14993..17002)
                     /gene="ligA"
                     /locus_tag="PLO58_00075"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQO73218.1"
                     /translation="MNLDALREKAKELRRQIETANYNYYVLDNPTISDAEYDRLMREL
                     EQLEAAYPELRTPDSPTQRVGAQPLDEFKTVTHRVPMLSLDNAMDEGEIRDFAIRVQK
                     SLPGETVQFVCEPKIDGLAVELVYENGVFISGSTRGDGITGEDITQNLKTIRSIPLRL
                     MPNLPIPTLLEVRGEVYMDKADFQRLNEQQLKEGKPLFANPRNSAAGSVRQLDPQITA
                     KRPLKIFCYGLGSLEGYFFQTHHEFLEALLKWGFRVNPLIEIAKEVEALIAYYHKIES
                     IRDSMPYDIDGVVYKVDSLAQQAKLGIKSRSPRWAIAGKFKAQQEVTQIIDIEASVGR
                     TGAITPVAHLKPVQIGGVTVSHATLHNQDEIDRKDIRIGDWVVVQRAGDVIPQVVKVI
                     TERRTGNEKRYSIPANCPVCGTPVVREEDEAKHRCPNINCPAQIKASIEHFASKRAMN
                     IEGLGEKLIEQLVDSGLIKNVADIYSLRKEQLLTLERMGEKSAQNLLDAITASQNVSL
                     ARFIYALGIRNVGEHMAKVLAKAFGSLERLSQASEEELMAIEGVGPIVARSVRNFFNA
                     ESNRQTIEKLLSSGIRISSEQDYKQKEEIAGKTFVFTGTLTNFTREEAQALVEKYGGK
                     SASSVSKKTDYVVVGENAGSKAETARQLGVKILNEAEFLELISYN"
     gene            complement(17173..17580)
                     /locus_tag="PLO58_00080"
     CDS             complement(17173..17580)
                     /locus_tag="PLO58_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73219.1"
                     /translation="MQSMKKIFLIWMFSTIAFAQQAVEVSDIIISDLDNNLIEIRVEV
                     KNTSMKSISELAGFIDIYDKYGNIIEKQEIVIVLNSDIPLKPQNTASGSIIITQRPNM
                     SGQVNFRITNLRYFGEPNVYLICPVCGELIPKD"
     gene            17998..19461
                     /gene="guaB"
                     /locus_tag="PLO58_00085"
     CDS             17998..19461
                     /gene="guaB"
                     /locus_tag="PLO58_00085"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQO73220.1"
                     /translation="MNEKIAREGLTFDDVLLIPSESNVLPKEVSLDAKLTKKIQLNIP
                     ILSAAMDTVTEGKMAIAIAREGGIGILHKNMSIEQQVAEVDKVKRSESGMIVKPITLS
                     SDSTIRDALNLMNRYSISGIPVVDNGQLKGILTNRDIRFETNLDLRISERMTKENLVT
                     APEGTTLEEAEEILQRHRIEKLLIVDDKGRLKGLITVKDILKKKRFPNAAKDEFGRLR
                     VGAAVGVGSDYLLRAEKLVSEQVDVLVIDTAHGHSANVIKSVRELKRRFPQVDVIAGN
                     VATAAATKALIDAGADAVKVGIGPGAICTTRVIAGVGIPQLTAILDCAEVAMKSGIPI
                     IADGGIRYSGDIAKALAAGASTVMLGSLLAGLDESPGETVLYEGRVYKSYRGMGSIAA
                     MTHGSSDRYFQEGEETSKLVPEGIEGIVPYKGSLADTIHQLIGGLRSAMGYCGTATIN
                     ELQKRGCFIRVTQASIQESHPHNVKISKEAPNYRLEN"
     gene            complement(19489..19743)
                     /gene="rpsT"
                     /locus_tag="PLO58_00090"
     CDS             complement(19489..19743)
                     /gene="rpsT"
                     /locus_tag="PLO58_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQO73221.1"
                     /translation="MKKNASVLKRTRQAQKAALRNRHYVSMMKTSIKKVKNTKTKAEA
                     EPLFKAAVALIDKVARKGIIHKNNAAHRKSALAEYLANLS"
     gene            20474..20845
                     /locus_tag="PLO58_00095"
     CDS             20474..20845
                     /locus_tag="PLO58_00095"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HQO73222.1"
                     /translation="MLEGLVDTLLSRLREIVASETVIGKPIETGDTMVIPITKISLGF
                     MVGSSGNPEEQKKKTEGTGLGGGALIEPLAVITIQKGEVKIHRLKEKVSGVDKLMEIV
                     PEVLEKYIKPKCEKKSAKENT"
     gene            20940..21617
                     /locus_tag="PLO58_00100"
     CDS             20940..21617
                     /locus_tag="PLO58_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQO73223.1"
                     /translation="MEIDILKNIPLFADLKDDIIQQIYNLMQKRAYKKGNIILMEEEF
                     GDTLFILSKGSVKITRMGEDGREVILSILGEGDFFGEMSILDGENRSANVVALEDTAV
                     LILKRGDFLDLLEKHPQIAIALLQEMATRLRRSDQQIEGLSLSDAENRIAMCLLRLAE
                     DLGVIKQGKVIIENLPYQQDIANMAGTSRETVSRMLTLLQRKGYIQKYGRRLIINNYD
                     GFVKQFS"
     gene            21889..22662
                     /gene="tatC"
                     /locus_tag="PLO58_00105"
     CDS             21889..22662
                     /gene="tatC"
                     /locus_tag="PLO58_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQO73224.1"
                     /translation="MARINSTNAEKEMGFLDHLEELRWRIIKILIALVLGTVGAFLFS
                     DQAIDILMRPARQVGSEMTIQVLKIQGLLMLKFKVAFLLGLVIALPIVAYQFWAFISP
                     GLLQNEKRWGPGLIIGVTVFFLLGAIFAFFVLIPIALKFLIGIGVAGVERNISIEYYT
                     KFVMQLILATGLIFQMPVLSFILTKIGLITPQFLRKSWRYAIIILMVIAAIITPPDPV
                     SMIFMTLPLLFLYEISIWISRIASRPKIQNETDQIELAG"
     gene            22669..23649
                     /locus_tag="PLO58_00110"
     CDS             22669..23649
                     /locus_tag="PLO58_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQO73225.1"
                     /translation="MPPKDLNNIFAPISTELDLFQKEFRQVLYSEVELINQIGSYILS
                     GGGKYIRPALVLLSANLCGKSNEHSIIAASVVEILHTATLVHDDIIDDSEVRRGRPSV
                     KAIWSNKIAVLAGDFLFSRALSNMLRLKDFTVLELLSQTSELLSSGELLQLEKSLNNN
                     MDEEIYYQVIWAKTASLFATACQAGALTVAANEEQTKALFDYGRCLGLAFQIEDDLFD
                     CTGTSIHTGKPVARDIKQNLVTLPLIHALKTLAPEERHRIRQQLKNRPSPEQITAITE
                     LIINTNGITYAENKCRELIQQAVAALAIFPDSQYKSALINLANYTLMRNN"
     gene            23646..24689
                     /locus_tag="PLO58_00115"
     CDS             23646..24689
                     /locus_tag="PLO58_00115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQO73226.1"
                     /translation="MTTTTNPNSILIMRLSSMGDIVVLTPIFRSLREKYPLARIDFLT
                     KMEFAEIVANNPFLSNIITYDSGTKLAGWRDLCRQLKAQDYDLLIDMHNNIRSHILSH
                     YLRGTSLLRYHKPYLRRFLLFYFWLNFFSHDFRLLKEYYKVLQPLGINYPPTAKPEIF
                     LEPEAVTKAQDILVKYEISEPFIIILPIANWPNKLFPLEKYINVARRINTDLQMPVLW
                     LGGKADIHLQQLDYLVPGKSVRLAGETTLSESLALIGLSRAVIGNDTGLTYAAEALGK
                     PVILILGPTARETGAGCYAPKSIVLEAQLFCRPCSQKGDRRCYRRQRQLCFNQITDAQ
                     VYDALVKIIGAES"
     gene            24686..25966
                     /locus_tag="PLO58_00120"
     CDS             24686..25966
                     /locus_tag="PLO58_00120"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HQO73227.1"
                     /translation="MTIFWLVIYNAIVMPIAYSGLMILSHFNSKIKEGLKGRQTLWLN
                     LQKFREELPKRTVYLVHSASLGEFEQAKPVIRGLKTLRPDILLVASFTSPSGFKNAER
                     MPEVDLFTYLPIDTFLQTRRFLQKLRPTKIIFVTYELWPNLIFHANRYKIKTYLISAR
                     IRPDSSKLKPIIRHFFADLYRSLDYIYAISEEHKQSVLKLIGSVKITVLNLGDTRYDQ
                     VIERSKLLANKMVTRVFNDGFVFIGGSIWPQDARPLLPALYQCLKNFPDFKIILTPHE
                     PNDYALEMLEDEFQTHNIETVRYSQLKGMPANVRVVLIDRIGILAELYHQANLAFVGG
                     SFRGSIHNVMEPAVAGLPVLFGPSYHNSQEAELLVQSGGGLVCLDEPGIYSTIKHLIT
                     KPDDYQKAAQSARQVILKNMGASSRTVQEILESE"
     gene            25963..26610
                     /locus_tag="PLO58_00125"
     CDS             25963..26610
                     /locus_tag="PLO58_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQO73228.1"
                     /translation="MKLRLDGIDFSYHGNQPVFKNLDLTIPLNCWAVLTGDCGTGKTT
                     LAKLIAGLLKPDKGSIRFENDNGLNNSLEVGYIFQNPDDQFVHFNIEREVAFNLENRG
                     ASPAIVRKAVQLALESVNLWPRRENSPHELSGGEKQKLAIADWLVSKPQVLIFDETTA
                     FLDLPTRFELYENIRRLIVAGLSVIWISQEDYEIAFADYIIELLPGYLLRTSSNK"
     gene            26629..27378
                     /locus_tag="PLO58_00130"
     CDS             26629..27378
                     /locus_tag="PLO58_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HQO73229.1"
                     /translation="MNYLICQNLEFQYPQNPNFRLSIPELTCPLNYPLGVYGLTGSGK
                     STFGKILGGLLTPTKGFFQIEAPEPNLSSSKTKPRILYLPQFPEQIFLGITIGNALQK
                     ILKSWNDPAELTAKFADNLQKLGLDYNLIRERYGYELSSGELRLATIALGITLAPQLT
                     IFDEPTIALSPSSRTKFENILPEYDSQKEFMIISHDYRLLRKLCHNILIFDKGSVIFE
                     GEWKILDQNFSIIHKVGLNLLEKSYQRNSNE"
     gene            27371..28009
                     /locus_tag="PLO58_00135"
     CDS             27371..28009
                     /locus_tag="PLO58_00135"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQO73230.1"
                     /translation="MSKNPIKHTLQVKRNITSKYDAIPARKTVTISNIISLVRAFLAA
                     PIVYSLRNNDSKTALIFILIAILSDMLDGWLARSSNEITVLGKILDPFADSVVIMSVM
                     LFLTVKERIPTFYFTIMLVRYFLISVFGIYMLNNSKVSPQSNKLGKVSVVFTVITVLA
                     FIYPNFFGSSAKPLMIVSIIFMALSLVQYIYEYSRQIIIASREKKANLSKND"
     gene            28002..28886
                     /gene="ftsY"
                     /locus_tag="PLO58_00140"
     CDS             28002..28886
                     /gene="ftsY"
                     /locus_tag="PLO58_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQO73231.1"
                     /translation="MINFGLNKTGRFLASRLPLLGRFFRLDEKTLEEIEEALIQTDLG
                     VELTETLIERLRKVDRKEAARLNEILQNELSALIQPAVATKTNFHKPTVILIVGVNGT
                     GKTTTIGKLAAKYASEGQRVLICAADTFRAAAYEQLRIWAERSGVEFIGNPQGKDPSA
                     IAFDAARAAQARNADILLIDTAGRLHTKSNLMEELAKVKRVIGKVIPDAPHEVWLVLD
                     ATVGQNGILQAREFQKAIGITGLILTKLDGTAKGGIALAIHRELNIPIRFLGVGEKLT
                     DLVEFDPNQYIRDLLNIE"
     gene            28983..30287
                     /gene="rimO"
                     /locus_tag="PLO58_00145"
     CDS             28983..30287
                     /gene="rimO"
                     /locus_tag="PLO58_00145"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQO73232.1"
                     /translation="MSLIPAVRSFAIISLGCPKNTVDSEILKGGLMQAGMEFKADPAE
                     ADAVIINTCGFIQPAKEESIETILETLRLKKTGVKKVVVMGCLSQRYYAQIKAEIPEI
                     DGLFGVESQSEVIRFLTGSQNICPDLETIRQLMTPRHFAYLKIAEGCDNSCSFCAIPL
                     IRGRQRSRSIESLLREAEYLKSQGVKELILIAQDTTRYGSDLPGKVSLKTLLEELLAA
                     RLFPWLRLMYANPDFWRDDLNHILAQHPEFCPYIDIPIQHASPRLLKLMNRGANPKRL
                     KATLRNIRRYRPDVALRTSVMVGFPSETEAEFEELLNFIEEIRFERLGVFTYSAEEDT
                     VAEKFDDDIPEPEKERRRDLLMQIQWEIGEDFARRKIGQKITVLIEEQDGDEYIARSV
                     WDAPEIDCNVRVKSPRILSIGDFYEVTIIGVENLDLRAETNG"
     gene            30364..31434
                     /locus_tag="PLO58_00150"
     CDS             30364..31434
                     /locus_tag="PLO58_00150"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HQO73233.1"
                     /translation="MKALITAGGHGTRLRPITHTQNKHLIPIANKPILNYALEYVRDA
                     GIQEVGIITNKEGNEVREVYGDGQAFGLKIVYIPQERPTGLADCVRIAEPFIGNEPFV
                     FYLGDNIIIGGIRRFIEEFERNQTNCHLVLAKVPDPERFGVPEIKGNRIVSIVEKPQK
                     PLSDFAVTGIYIYDHTIFEAVNNIKPSARGELEISDAHQYLLNKGYKVTFSEITGWWK
                     DTGKPSDLLEANRLVLDNITTSIQGKVDQNSQLSGRVVISKGAEIINSNIRGPVIIGE
                     GAKIVNAYIGPYTAIDKNCEISNAEIEFSIVSCNCVIRNQKIRIEASLLGKNVQIIES
                     QAKPFTNRFIIGDQSIIEIRQT"
     gene            31431..32726
                     /gene="mtaB"
                     /locus_tag="PLO58_00155"
     CDS             31431..32726
                     /gene="mtaB"
                     /locus_tag="PLO58_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HQO73234.1"
                     /translation="MIATVAFRTLGCRLNQAETDAIKAELANRNLHIVNSNKPADLTI
                     INSCAVTQAAEAKTRGAITAARRISPNGKIAVIGCYSQIAAAELLNISGVFLVLGNNE
                     KYEIFDFLETIERGEKIVRVTKPESPKTFRIESHLPNDKRIRANLKVQEGCDYYCAYC
                     IIPHLRGNPRSRDPEDCIATARLLENQGVQEIVLTGINLGRYESPSGDLFFLIRRLLA
                     ATGLPRIRLSSIEPDLITSELIKLISEEPRLCRHLHIPLQHGSDPILAKMNRRYLSAD
                     YATLINRIKQQIPEICIGADVMVGFPGETDLEFDQMRQFLENLDINYLHVFRYSPRPG
                     TPAGKFTDRIPDSEKKRRSEILRQLSNRKRAAFRQSMLAKTVSVLFEEKIAPGLWQGL
                     TDNYLKVRIHTRENLLNKIEKVKLIHVSEDYIEGELLGE"
     gene            32719..33342
                     /locus_tag="PLO58_00160"
     CDS             32719..33342
                     /locus_tag="PLO58_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQO73235.1"
                     /translation="MSEHPYFIGIAGNIGVGKTTMTDLIARRFGWRAFYESVIDNPYL
                     EDFYQDMKRWSFNLQIYFLSHRFRSHKQMTESNITTVQDRTIYEDVEIFARNLYEMGN
                     MSQRDWDNYRALFEIMTSYLKKPDLIVYLRASVDTLLTRIKKRSRGFERDISPEYIYT
                     LNLSYERWIKEEQKRSNILIVETDQLDIFRDQEKLEEIMQKIKQFCP"
     gene            33456..34304
                     /locus_tag="PLO58_00165"
     CDS             33456..34304
                     /locus_tag="PLO58_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HQO73236.1"
                     /translation="MPIDFTKITIFKPCSFLVKQDDLITLMQEITGYPGAIRFSIEKN
                     NGETPSLAQIFQMRQNNKQLISPNEQYHQLVLFQHFLRELTAALKYNILKNTSVAFRM
                     RVELANLLKENRALIQPAAIVRLVDCLPTETGLALAGGTYELHGKSLHRFVNKSDPVK
                     PKPIFIFIVTIGSAIDKRIADLNYTEGDAYQAFLLNGLAAALVETATSDLQQYLEKSS
                     SWLSPSQTLRRVSPGFRDWPLTDQKVIFEILKPSENIGVTLSADCLMQPLKSTSGIMG
                     VIENAL"
     gene            34551..35306
                     /locus_tag="PLO58_00170"
     CDS             34551..35306
                     /locus_tag="PLO58_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HQO73237.1"
                     /translation="MAPKNNSQPIYNLFPRIGNPLSLSQEIVEHIEELIRQKKILPGE
                     KLPSETELCKMFGVSRTSLREALQKLSARGLLRIRKGSGIYVTDYQAENVIKPMSLFL
                     ELNLDKDYLMSVMEIRKILEPQIASLAAARRTARDLTKLRKNCNDLKKCDSTDYLKQG
                     QIDKDFHLNIAIASKNQLVPIIVEPMFRLMPKIRSLVYQQIDSAKSSAITYHDLILEK
                     IQVKDSQGAFEAMTTHLQIAEEHTLQIITDLAK"
     gene            35463..36113
                     /gene="eda"
                     /locus_tag="PLO58_00175"
     CDS             35463..36113
                     /gene="eda"
                     /locus_tag="PLO58_00175"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HQO73238.1"
                     /translation="MDRQTITAKLIESGLVAVIRLDSGEKLPGIISALRQGGINALEI
                     TMTTPGALEIIKNFAAQTGKDFLIGAGSVLDAETARLAILNGAQFIVGPIFNPEVVRM
                     CHRYDKPAIPGAFTPTEILTAWEQGADLVKVFPTTALGPSYIKDILGPLPQVKLLPTG
                     GVTLENAAEFIRSGACCLGVGTALLNKKMIAADDWQALAKHAEAFRSAVLTGRSSK"
     gene            36136..36966
                     /gene="kduI"
                     /locus_tag="PLO58_00180"
     CDS             36136..36966
                     /gene="kduI"
                     /locus_tag="PLO58_00180"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HQO73239.1"
                     /translation="MEIRYLADPIRFQRMTTSELRQNMLVENLFQTGELRLVYSDADR
                     AIVGAAVPANKPLTLSGSKELAAEYFTERREIGIINIGEAGIVSVGEESFHLAHKEAL
                     YIGKGRHDIVFQSDSTQKPAEFYLLSYPAHQAYPTRKVTQAQANRRDLGSDAECNKRT
                     IFQYLRPGVLDTCQIVMGLTELAEGSVWNTMPPHTHERRTEIYLYFDLPKNGLVFHLC
                     GEPESTRHIVMHNKQVVLSPSWSIHAGVGTAHYTFIWGMGGENQDFDDMDDIDPVNLR
                     "
     gene            37080..37700
                     /gene="purN"
                     /locus_tag="PLO58_00185"
     CDS             37080..37700
                     /gene="purN"
                     /locus_tag="PLO58_00185"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQO73240.1"
                     /translation="MKNIAVFASGRGSNFAAILDKIQKGEIAARVVCVISDNERPPVF
                     EIANAAGIPNFCLNRKQFATPEEYANALLNVLEKYDVDLIVLAGYLKLIPSPVVRKYP
                     RAIVNIHPALLPNFGGKGFYGMKVHEAVIASGVKFSGVTIHFVDERYDTGSVICQEKV
                     AVLPDDTPESLAARVLEVEHRLYPEVVKALCEDKIKFVNGKVVWEK"
     gene            37697..39247
                     /gene="purH"
                     /locus_tag="PLO58_00190"
     CDS             37697..39247
                     /gene="purH"
                     /locus_tag="PLO58_00190"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQO73241.1"
                     /translation="MSEFKIKRAIISVYDKYNIGNLAQALARKGVQIFSTGGTYRALQ
                     TAGIAVEKIEDLTGFPEILDGRVKTLHPAVFAGLLADATDESHRADLARVKVEPFQLV
                     VVNLYPFSETYHSGNKSFEEIIEMIDIGGPSMLRAASKNFKNVVILSDPGQYDEFLQR
                     LDQDELTLEYRQKLAAAVFLKTMTYDSEISNFLTGAGAEKMPEVIVQAFHKQQTLRYG
                     ENPDQSAALYAPLNNPNWQPFQQLQGKEISFNNYVDCLAAYKCYATFKTERAAVVIVK
                     HTNPCGFAIGDTTLEAYRRAVQTDPVSYFGGIVGVNREIDANLAEEFTKSFLECIIAP
                     SYSNQALEILSKRKNLRLLMPNAEALHYDLDLRSYGQGLLVQEIQAAEDDESQWKIVT
                     ESQPDPSLMPTVRMAWHVVKHLKSNAIVFCDANGTVGIGAGQTSRVDSVKIAIRKAGE
                     AGLSIKNTVMASDAYFPFRDSVDIAAEQEVIGFIQPGGSIRDQESITACNERKLFMIF
                     TGKRVFKH"
     gene            39264..40277
                     /locus_tag="PLO58_00195"
     CDS             39264..40277
                     /locus_tag="PLO58_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQO73242.1"
                     /translation="MGLNLFNIFTGDIAIDLGTANTIIYMKGKGILLDEPSIVARSTH
                     DGEVLAVGADAKDMLGKTHHDIMTIRPMKDGVIADFEMTDAMIRGFIRKINLSRIARP
                     RMVICIPSGITEVEKRAVRDSADRANAREIYLVDEPVAAAIGIGIDISKPVGNIIVDI
                     GGGTTEIAVIALNGIVTKQAIRVAGDEMDEAIVQYFKTEHNLLIGERTAEYIKCSVGS
                     AMPINDTTIQVKGRSLVAGIPKTIEVSSVEIRECLKDTLDLIIQAIKQALENTPPELS
                     SDILDRGIILTGGGALLKGLDQRIRVETDLPVNVAETPLHSVVKGAGMIIEDPEKYRD
                     VLV"
     gene            40283..41071
                     /gene="mreC"
                     /locus_tag="PLO58_00200"
     CDS             40283..41071
                     /gene="mreC"
                     /locus_tag="PLO58_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQO73243.1"
                     /translation="MWLKIIRFIIDHYEYLLLIFLIFLSLFILAHNNSPQIRATQGEL
                     ADAFAFLHYPKVWINELSNLVEENKELKEENLRLALLNAQLKEAYLENQRLREMMNFA
                     DTSRLTILPAEVISQGTTPLYNSIMINVGSKHGVGPNMAVITNDGVVGKTVAVGDNNT
                     LVQIFLDVNFRLGVKFQISREHGIMRWRPNGLAEVREIPKTAIIYPGEKVLTSGFSEI
                     YPPNIIVGEVVEVHQSANNLFQIATVRPSVNINSIEEVFVVLSK"
     gene            41071..41550
                     /gene="mreD"
                     /locus_tag="PLO58_00205"
     CDS             41071..41550
                     /gene="mreD"
                     /locus_tag="PLO58_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HQO73244.1"
                     /translation="MLRKILLIIAFSLVTIIIQILLGPWMTIRAIRPDFLLILVLFVG
                     RSEGKVAGQIYGFLIGLIADALGLGSFLGLSALAKTIAGFGAGLLKKRRSHLNPILFY
                     AIEVLIIIIHFSIIYLINFKGAELSTQTIFLRYILPSTLYTGVFYFIIQYFAPFSLE"
     gene            41554..43359
                     /gene="mrdA"
                     /locus_tag="PLO58_00210"
     CDS             41554..43359
                     /gene="mrdA"
                     /locus_tag="PLO58_00210"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQO73245.1"
                     /translation="MYISHNSITNIRKNILTIFFVLVFLVLIGRLYYLQIIKGPSYKG
                     IAEANRVRVIPIEAPRGIIYDRNGKIIVDNKSQYDIDVIPYEFSHTARTDSILAKILG
                     LDVAEINSRIKNNWRGQFVPIKIAEDVNVEIITNIEEHRLDLPGVVYSVKPVRSYPSG
                     VQMSQSLGYVREISANDLIREKYKEYKQGDLIGWKGIERQYEHILKGTRGYNFIQVDA
                     LGREIGQVVDRDNIPPIPGNEMFLAIDLDLQIYIESLTEGYKAGIVVLDADNGEILAI
                     VSKPDYDPALFAGVISNSSWNALISDPDLPMFNRATQGTYSPGSTFKLIAVLTALETK
                     AVDPDWSVRCYGQYRLGHRIFKCWQERGHGSMNMYEAIVHSCNVYFYNLIRRLDLNDW
                     ANMGKDFKFGSETGVDLYGESAGVMPDVDFLNRKYGEHGWTDGNKLNLVIGQGDILVT
                     PVQMARFCAALASRGKLVQPHVGLKYIDRGSNETITFQAAEDSIQNISVDTWEFIEKA
                     MADVINRGTGRAARVKDVPVWGKTGTAQNPHGDAHAWFIGYMKEQNQTIATAVLVEHG
                     KSGGGIAAQIAGQIFNFIHQQKKRQSLANNAPLNF"
     gene            43394..44590
                     /gene="rodA"
                     /locus_tag="PLO58_00215"
     CDS             43394..44590
                     /gene="rodA"
                     /locus_tag="PLO58_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQO73246.1"
                     /translation="MNLAILVIVGLLCLFGMLSIYSASLNMEGSFFEKIIGRQLIWLV
                     LSGLVAATIYFLQKKLIYDSAYILYLLGFCLLLLPYFLGSSVAGTARWITFGPAGFQP
                     SEFMKIMVMLAIARFLASTSSSNLDFKFVLIPTLLSLLPMAVVLKQPDLGTSIIYFGM
                     LFPMLYWAGARLFHLFIIIAPILSIITAFNFYTFFIWVIIVVIILYLTKEKLRVSLTV
                     MLINLSLGFTTPFLWNSLKPYQQQRILTLFNVNADPQGTGYQVLQSKIAIGSGGLFGK
                     GIARGTQTHLKFLPEQHTDFIFSVIGEEYGFIGVLVVLLLFLAFILICINIAYRQKDR
                     FCSLCMIGIASIIGIHVVINVAMTVGLMPVTGLPLPFISYGGSFLMTCFILVGLALNI
                     GVDKQY"
     gene            44675..45577
                     /locus_tag="PLO58_00220"
     CDS             44675..45577
                     /locus_tag="PLO58_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73247.1"
                     /translation="MQQPIKNNLKFKPSAIKLFVLALSLMVINLDLARSDSKRLSNAE
                     IAASLDRLAMDISDLKTQIANLTKTVNSLTAKDKESITKATLTTDINKLIEPLQLTMA
                     NLENRLNILSAEQANMQKRLNTVEQRTRYADSINFEILSQLVILENRLASLSNSISDY
                     NSASQTDARQATNRAITSPGITYKDRYLNALSLHQKGKFEEAIALFRKLIADDPTNEL
                     ADNAQYWIGESYYSMKQYQQAIIEFEKVRNFTNTDKDDDAQYKIGLCFKQMGDQKRAK
                     DELEKLINLYPTSEYSERAKQLIK"
     gene            45586..46422
                     /locus_tag="PLO58_00225"
     CDS             45586..46422
                     /locus_tag="PLO58_00225"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQO73248.1"
                     /translation="MIKRIYFLTSEIIPFAETYQLAIFSKEIPMVFNERKYDFRLMMP
                     KYNFISERRYILREVIRLREMDLQYRGENIKASVKSAFIPNTKVQVYFLEHPYYFNNS
                     DKNLYPTECVSDEYLNSVRFGYFAMAALKTLSYLRWKPEVIICNDWQMSLIPLFIQSN
                     LVDKEYCEGIKILQIIHSKVDCSNFKVTEYQKIGLNNLDLSKSVDGRLDVVAAAMPYC
                     DHVLIINKNENLLEKYQKDPIFSEIMKQDKKKFSSYELNGESPEDWQKLADEIINIME
                     QL"
     gene            46391..47641
                     /locus_tag="PLO58_00230"
     CDS             46391..47641
                     /locus_tag="PLO58_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73249.1"
                     /translation="MKSSILWNSSKPYFNRVWSKLHYVKIDYRRSVAAFFCLLLFGCS
                     EKIPTLPLDKNLCSFDSVSVSAEFIVDTTVFAEPNIGYSSYLVVGTDANLTAYSLLSF
                     KTLPTPLDTLPLAKILSCELVLTTGSDLSSDTAVHPSLEMTVASIEGEENADWSEDST
                     NSTNFSLDDYHLTDWKTFTYSDADTLILDLTTSFIANWRDSTQSHYGIVIKPAVGSAA
                     GLGIIYSGETNSSPHIIISYITDNGDTISTALACVGDVTITQFKKTFTAPPQPLLISA
                     GKAAYTFLKFQVEELITDKNLFIAGAYLYLHVNSELTEDYGNSHTIYITLLDSAEWES
                     GSFSPSGSSYVTSQTFSASDSILILKIPSTVQLFTSGNISNFGVALWISPSGLYPAML
                     GFYPTEDPDPARRPRLEILTMQEE"
     gene            47638..48861
                     /locus_tag="PLO58_00235"
     CDS             47638..48861
                     /locus_tag="PLO58_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73250.1"
                     /translation="MKTSRRTILFLLILSLNGWSQISYYANYGFGVESPANSVRILGM
                     GRIGTAVRDSMSLNQSNPALWQGFGSTSLQGQMYTSALSIPDLDFKGRIVNFSGFSFK
                     LPVGKRAGLAIGLAPLSRMKSSVSFADSLIFQDDKFEYNSVVELSGGISELYLGSGYR
                     VSPNLAIGLRANFLFGNYVVRNHTKIDSDIDSESYYKRTIAVDGSRISFGLLLSDRSQ
                     RYNLAATVEKGNELKRWTIIENNFGPDTTIKSDPINFPTIIKVGINLPIARNLALNAD
                     MQYGIYEEDLFQNFYIFQQFKSDSRNSYAVGIGLERSPRQELKSTFFQKLYYRCGAYY
                     QTEPVYQNSGIYEYGVTAGISLPFFYNLNRIDFALTYGLKNGFLSDEIGNEKILGIHL
                     GITTGEPWFRRYKKQ"
     gene            48880..50166
                     /locus_tag="PLO58_00240"
     CDS             48880..50166
                     /locus_tag="PLO58_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73251.1"
                     /translation="MKRWIHFIEEALIVCLVIGLMNCTPPSPESSESVMEQVETKPTI
                     DRDKCDLYLSFAYSYYQNQNWRSAIENYKKMSSFGCEEEYAKDLFPFWGRAYQQLASE
                     NSVYLDSALYVYLAGEKYLPNDLFLKKNIAYIYHLQGKIDLEIREYEKMIEIAPDDIE
                     LYRTLVKLYISAQRYEDVIYATNGILKINPNDEQALNDRIFAYQKLGRDITTIQKEQW
                     EKNPTNIRYGLEYAAALKQQREYDKALEVYQKVAELDAKNIEAWSNIADIYFTQKNNY
                     AEALKAYDQILRNISPNDLNILQQAARCCQLLGDFPKGMEYARKAITIAPNSPQAYKI
                     RADLYFAAADYYTSGRQITFEDKLVYKLAYDDYLKVMELGDTSVKARVDHLKEYLIPT
                     PEEWFFNRYDANGAMRTSFRPLSPEYKWITVEPKKN"
     gene            50136..50609
                     /gene="rpiB"
                     /locus_tag="PLO58_00245"
     CDS             50136..50609
                     /gene="rpiB"
                     /locus_tag="PLO58_00245"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HQO73252.1"
                     /translation="MDHGRTEEELNMVIAFGADHGGYRLKNALIEFARSEGYQVIDFG
                     VNSDDSADYPDYARAVGEAIVSGRAQFGVLICRTGAGMCISANKIHGIRAVMAYQPEI
                     ARLSRLHNHANVLCFGADFTTVESAKEILKTFLETPSSDDLRHLRRVEKIMKLES"
     gene            50614..51918
                     /gene="glyA"
                     /locus_tag="PLO58_00250"
     CDS             50614..51918
                     /gene="glyA"
                     /locus_tag="PLO58_00250"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQO73253.1"
                     /translation="MEYNFLAKQDAVLLDTIKAEIERERKTLEMIASENFVSPAVLEA
                     AGSVLTNKYAEGYPGKRYYGGCAAVDQAENLARDRVKELFGADYANVQPHSGSQANMA
                     VYFSLVNVGDTVLGMDLAHGGHLTHGSPVNFSGKLFRIISYGVKKDTGRIDFDRLAEL
                     ALEYRPKMIIAGGSAYPRFLDFAKFREIANSVGAFLVADVAHPAGLIAAGLHPDPLPY
                     CHVVTSTTHKTLRGPRGGLILMGKDWENPFGLVAPKSGRTKMMSEIIDSNVMPGIQGG
                     PLMHIIAAKAVAFGEALRPEFKAYAQQIIVNAQVLAEELLHRDYNLVSGGTDNHLILI
                     DLSNKNITGKQAENILEESGITTNKNMVPFDQKSPSVTSGIRIGTAALTTRGMREAEM
                     RTIAGLIDHVLSNPDSKDIHQKVRSEVGELCGNFPLYQEIKL"
     gene            51926..52378
                     /gene="nrdR"
                     /locus_tag="PLO58_00255"
     CDS             51926..52378
                     /gene="nrdR"
                     /locus_tag="PLO58_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQO73254.1"
                     /translation="MRCPFCSSPESKVIDSRTVQKNNAIRRRRECIDCGHRYTTYEYV
                     VEYPLQIIKNDGRREEYDRNKLLNSIKIACNKRPIPAEKLETLVIDIEKEIEETSGSE
                     VPSSQIGELVMKKLKELDEVAYIRFASVYKKFKDLDEFKDHINKFGGN"
     gene            52467..52631
                     /locus_tag="PLO58_00260"
     CDS             52467..52631
                     /locus_tag="PLO58_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HQO73255.1"
                     /translation="MLSNELLEILACPQCKGELEYDISNQQLICNVCRLKYAIKDDIP
                     IMLIEEAEKF"
     gene            52779..54002
                     /locus_tag="PLO58_00265"
     CDS             52779..54002
                     /locus_tag="PLO58_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO73256.1"
                     /translation="MKRIIAMLGIISLMTAGALAQGEAGAVWLLINPGARFSGMGEVG
                     AALADDAYASYWNPAGLGFLRGRELALTHVNWLPNLVSDIYYEFIGYRQFIRNVGTVG
                     GNIILLNLGEQQRTDEFGHDLGTFNSFMLAVTGSYGTALTRTSSIGVGVKMVHQHLTD
                     QGAGKELGKGYSTNFCFDIAYLKHSFLTKNLTLGINMANIGPKIAFIDVNQADPMPTN
                     MKLGLAYKLGNEYNKVTIAFDINKILVAAYPDRDIDGDEKVGGYDENGHAVPGGEYNK
                     KGQREYAHSDEWYTGIFTSWFDDWLLMYDSATDGIGSADKDGNTNDGSLAAEFEKLTY
                     NFGIEYWYSNLFALRAGCMYDKPGNITMKGKWPVPTFGAGLRWAGMGFDFGYTAGDPG
                     HPRQNTMLFSMNIKF"
     gene            54060..57194
                     /locus_tag="PLO58_00270"
     CDS             54060..57194
                     /locus_tag="PLO58_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO73257.1"
                     /translation="MKIYKYLITGITLIVLANKAAYGRFEPQYRIATAPSVVKNTAPA
                     AIRICALRVSFPADEDETTTGTGQFLMSAVETPCDDEFVVDPAPHNRAYFRDHIRSLA
                     NYYNRVSRGKVVIDTTNSEVFPLNDNNSYQVDHPMAYYHPFLVEDSVDLRLTELLVES
                     VQKADPEVDFAQYDVVIVFHAGVGQDFAITLDPTPYDILSAFMNKDDLKAFLAPNDSD
                     FQGIPVENGTAFVDCGLILPESQNHLLYDNWEEVFGESDSPCHYQFGLNGTLAFMFGF
                     YLGLPCLFDTESGMTGIGRFGLMDQGSANLSGLVPAVPCAWERVFLGWETPVVAQKWE
                     DVVLKKVGSGSDTTVWKIPINANEYFLVENRCAHFRPGVSFDSLQYRIYMENGMVEWP
                     LSFPLIRDSLKAEFSPETGVMLAVPHYDYGLEGSGLLIWHIDESVINANLATNSVNGD
                     REHRGVDLEEGDGAQDLGYEADILNKDIEDGWFFDPWFAGNDGFIDLNSDYILDVEEH
                     VGFTPYTNPATYSNNYTFTGISIDSIGPAGEVITFRIRLNDALPNFPLSLSAGADEIL
                     PIDLNPNDQVEEFLILADSIRIYTSFGEQIASLPRQTAIALDKDSLLLYGIVQNNPEA
                     SSLSLSIWKFGQNGQINKTDTVSLNSQDLVSPLLPVKGGVLFATQDYADNQNYLHLYQ
                     IDTQNIESLELASQVKMLVGDDDNIFGITDDNQVIRIHLNSLSAEVVADLPNALSGTM
                     VIAFINQNESVDLIHCADSLLTLVLDIGTPNEVVLHREQEFNGNLACADLDDDGKIEI
                     ITATSSQVLAFNEELTVKNNFPIGVPNILGSDRFESELLTADLDNDGKKDVIINMSSA
                     LLAFNYKGDLLSGFPKIAYQQSQKSGILLNTPLGITYLALTSTESNPQNYNCIKAVRL
                     SSFSHDSEDWICYGGNAARQFYYPVQSQSSPIQSKSLLDNARTFCWPNPPKDNRTYIR
                     YFPNSSCNISINIYDLAGDLIADFKDSAPLIGEPNEKEWDVSNVESGVYFAVVQAKSS
                     SRTDSKVIKILVVK"
     gene            57194..60235
                     /locus_tag="PLO58_00275"
     CDS             57194..60235
                     /locus_tag="PLO58_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73258.1"
                     /translation="MSERQICRNTLVIFLIMLFFFNPAGAQFGTYNHPELEWLSFETA
                     HFKIHYHQGAEWTARRTAEVAETIYGPITEFYQFEPSEKTALVIRDTDDISNGASYYY
                     DNKIEIWATPLDFPFRGNHDWLRDVITHEFTHIVSLQKAMKFGRSIPAIYIQVLDDEP
                     EKREDVVYGYPRIIASYPISGMVIPMWLAEGMAQYMFPGNSNDFWDTHRDMLLRDRVL
                     HKSLLSLSEMGSFGKRGTGNESVYNQGFAFTRYLAEHYGTETIPRLAEELSKPVRMSG
                     ETALKNVTGVAADEIYQQWQTELEQRYFKQTEIIRANEKVGEIILAEGTMQLGPEWSG
                     DSLLYFMSNKGRDYYSQTALYVYDFHQKKSRMLVPRINSRVTVAPDGQNLYYSRASKP
                     NRHGSVFFDIYRYDLKKRKEKPLTKSARVYNPTLSPDGEIIAFVTGRDGTSNVALMNL
                     DSNKIREITTFSEGVEVFTLHWSPDGRTIAFDYLIGHGRNLALVDVETGAMQILDEAA
                     YDTRNPYFSPDGKWLYFASDETGIYNIYRQSLTTGERQLLTNVTGGAFMPAVNEKGEL
                     VYAILDDRAFKIACIKNPEPIEPQLAVYLDYDQNIPPVSEIQPVSIPEPQKYHDQFAK
                     MFIMPRLMIDYGTVKPGFYCLSNEIINRFNIFAGASINRIKDRDLFLILEYHQWEPTI
                     FLEFYNISRNIFNEKSIYKGWPVEADYTFYLTEAYGGISRPFGGFNNVRFDVKWSRYR
                     TSSDETITAADIYQNGFSYDYYRGVNFQLHWQMRMMLPKVNEETNPDNGILLDTYIAR
                     NYDKFIKGFKVNSSYSTIQVDFRNNYYWRLEHEGNWYHKYPLFKNLVGTLRWHLGWIS
                     KPDVDSFFYFFAGGEPGLRGYPFYSIEGRNLSSWHYTWRLPLFREKHFQILTFNLQNA
                     FVATYIEAGNAWNQVTGFPGLKWEQFKTQPVSVLRNIVNDFKRDIGFQVRMSGFSFYS
                     YPTSISLDFVYGLDDFEVVSNSKTYQYGHEWRTYFTLLFGL"
     gene            60246..61148
                     /locus_tag="PLO58_00280"
     CDS             60246..61148
                     /locus_tag="PLO58_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73259.1"
                     /translation="MKSKFLAATLLCAILIPIMGFGQSFFDERTTVTERDRLIYSMKY
                     AQADEDTFVFNYEKSPGLAFFLSAVVPGAGEFYAGAKYRAAAFFSVEVVSWVGYFNRK
                     NNGEKLERKYMNWANTYWDAWQWYENSLSYPEYFGLSDSGSGFGSHTIYFEYEGKEYK
                     ADVSYLDRELPGWQTYLQEGTLRPIKTRDYYENIGKYDQFASGWSDFDQIVSEVDSLN
                     IPTSPLRKKYLNQRYESNQSLKMATNFTTVIMFNHLISAFHAQIAAKNYRPPEQKDVS
                     WHVGLVTDCTYKMPIRGLSLSVAF"
     gene            61193..61993
                     /gene="bamD"
                     /locus_tag="PLO58_00285"
     CDS             61193..61993
                     /gene="bamD"
                     /locus_tag="PLO58_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQO73260.1"
                     /translation="MPPKRQILKIVLISLLIIYLNNCASNKPKPVVDFEQHYRQGTEY
                     FNKKKWDKAIEHFTLVVINSPGSELADDAQFYIAECYFKRKEYLLAASEYQQLINRYA
                     YSEYVEEAFYKIALSQYKSSPRYQLDQEYSIYALQSFQDFIDTYPQSRFRADSEQKIA
                     EIRSKLARKLFESGKLYRKMQEWKAAIIYFDKVLETYYDTEWAEKARLEKAYCLIRQR
                     QFDQYELLFKEILARSTPQTDPNALEYLRTSYHKELRKIEREKRKRAD"
     gene            61990..62583
                     /gene="nadD"
                     /locus_tag="PLO58_00290"
     CDS             61990..62583
                     /gene="nadD"
                     /locus_tag="PLO58_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HQO73261.1"
                     /translation="MKVGLFGGTFDPPHNGHLEIARAAIRAFSLNKLIFIPANISPTK
                     TSEIHTSPVHRLMMLKLALQNEPACEISTIEIERGGISYSIDTIRAIKHQMRLEREDL
                     FFVVGADSLIEFHKWRQPEDILQEVQVVVARRPGCDLSLVKPEYLAKVLFFNAPLIPF
                     SSSDIRQIAMNGGQIDQFVPPAVAEYIRQNELYRRSA"
     gene            complement(62719..63447)
                     /locus_tag="PLO58_00295"
     CDS             complement(62719..63447)
                     /locus_tag="PLO58_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQO73262.1"
                     /translation="MSLPLNSPPANMASLVSQFDKLNSQYNLIADNQKILIAVSGGID
                     SLVLSRLLFELRLAEFKNLELLAAYVRQKSVALTTEALKEIADLLADWQIPFSILDSE
                     LPESSQINCYACARERRKRLFQFAIMHDCNAIAFGHNLDDYLETGLMNLVFKGNLESL
                     QPIQIQLGGRIRIVRPLLNWPKKHIRASGRAYGIKSIAPVCPMASFNKRQDINAMITS
                     FSRLNRGFRSNLRRAINHWNRLSI"
     gene            complement(63416..64519)
                     /gene="ychF"
                     /locus_tag="PLO58_00300"
     CDS             complement(63416..64519)
                     /gene="ychF"
                     /locus_tag="PLO58_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQO73263.1"
                     /translation="MGFKCGIIGLPNVGKSTLFNALTHAGVAMENYPFCTINPHIGVV
                     NVPDKRLDLLAQIYKPDKIIPTTLQFVDIAGLVRGASQGEGLGNQFLSHIQAVDAIIH
                     VVRCFENPNVAHIDEKLDPQRDIEIVETEILLKDLEIVENRREKLIARAKSGDEQTQK
                     QLKILDDIRGLLIKGQSAKQYHSHPEEEYFIRDLALLSSKPVIILGNISEDEAISGNK
                     SAITEAFEKFALQTGNLFLTVSANLELELSELPPDDRKLLMNEWKITEPGLDRLIRAG
                     YELLRLITFFTMESKIVQAWTIPVNTPAVKAAAEIHTSFEDRFIKAEVRHWKDIESLR
                     SENLLREQGLVHIEGKSYLVHDGDVITFKLAAG"
BASE COUNT        18328 a        13642 c        14270 g        18256 t
ORIGIN      
        1 aacgactgcc ggatgaagat gaactactgg atgaagacga gctgctggac gatgaggagc
       61 tactggaaga gctggctggt tgataagatg aaccgctaga gcttgatgaa ctcgaagagg
      121 aattgcctgt atttgaggaa ccggagctgg aggaagttcc ttttctaacg gtggcacgag
      181 gcgaactgga tttctgcgat gcggttgatc tagttgaacc gcgggaattg gtgtttgtgg
      241 taatcactgt ggttttagga gccgaagttg ggactaacgt ggtctgaaag ccgacgacgg
      301 ctttttcctg ggaactattt cccgctcctg tcgtcttggg cgaataacga gctttggtcg
      361 agccggaatt ggtggtagtc accgtagttt tttcgcctga tacggacgtg gtcgttatgg
      421 cactggtgga agtaggcagg taagatgcac cactttgaga accagcatca tttgaagaac
      481 cacgactgcc actgggagtt gacttcggga cataatcagc ttttaactca tcacgtcctt
      541 cgttgtagta ggttccatcc cgatatccat gccaatgatg cctatgatag gggtatccgc
      601 catagtaata gccatagtag ccgtaatgag ggtaatatcc ataataggga taataaccgc
      661 cgtagaagga aaagtaacca taaaagcccc agtcataaaa cggatcgtaa aaccacggat
      721 cataatatcc gagatacggt gaatgatagc gatagccacc ataaaatggc gagtaaatat
      781 tgaaacctaa ccagagttga ctattcattg agtcccaata accatcacgg taaccaagac
      841 gataatcatt taaataagaa gagtcaaatg tgtaattctc ataagtttct tgttcctggt
      901 aagttgtatc ggtaactgat tgttggtagg tttcttggcg gacgatcggc gcggaaaact
      961 gagtataaca actggagata atgagggcgg ccgtcattaa aataatttga gttaatgcgt
     1021 tcgctttcat attcattcct ttatttttag ttttcatagc atttaattag accgtgagat
     1081 aaccaagttt aatccatata tcaatgataa taagcaatta gtttgacagt ttattttcat
     1141 tataggttaa attgcactcg ctctaatata aaaaagttcc ttcaactagt tcaactagct
     1201 tatatatttt tctccggatt tttaccattg atgatcaagc tggctggtaa tatttgaaaa
     1261 ccatatttgg tttcgatgat accccagaac acaatactgc gattttgcaa ttttggtaaa
     1321 gcttttatgg gaacaatcat ttgattccat tgattaggct ggcaatcttt gaaaattaat
     1381 tcgcgatcgt aactcacttc cctgctttct gcagaacata ggagttggag ccgtacgctg
     1441 tcgagctttt cggcgtacag aggtttcaaa tcaatttgta aagcggcttc ttccccaggc
     1501 attaaaaagc cgtctccatt ggaatcaaga aaagacaaac tggcggcaaa accactagct
     1561 tttttatcca caggctggga ttgatagaaa atatcatccc ctttcttggc aggcaaaacg
     1621 tcaacgtaaa aagtctgtga atcaatgcca tcataattat cctgaacagc taccgtgaac
     1681 tcaaaccgac cggaatcgat cggttgccag accaaaatac cggtattttt caggattgtg
     1741 gcattttccg gaccggacac cacagaatag gttattttat cgaacagatc aatttcaacg
     1801 gcttgtactc gatagaaata aagttcgcct tgccgagcgg tttcgactgg gacgctcgta
     1861 attaccggcg gattattgat attagtcggc acaaaacggg gcgatccttg aatatttggt
     1921 tcagaatagg ggaaaattgt ccaatttgaa accgggggcg attttagtaa ttcagcctcc
     1981 tcgatgttct cccaacgcag gttagctatg acaaggtcac ccttgaatat tccgcgatag
     2041 gtaaaaggat tttcccgaga aaattgcggc acagtattgc caacgtaaac gacattccgg
     2101 ccggttggtg accagtccac cgaaaaggct aagtgtttta catttttgac taatgtatca
     2161 ggttggatgg aaattgatgc aaaagccgtg tcatataatt taaacccgaa aggttcaccg
     2221 tttggacggc tggcgattgc ccattcgcca cttggtgaaa tcgtccagct cagaagcggc
     2281 tcggcaaata tttgcaatat tttggtcttt agcgcatcga aatcagtctg gttgacacga
     2341 acctgctcgg gttttcgatt ctcaagccag ataaagccca cccgatcttg ggtcatcatc
     2401 ggaaatatat taatcccgga atggcttttt gtcagaacac gatgtcggtt ttctcttaaa
     2461 tcatacaaac gaatattacc atccaatcca cccgtgtaaa gtatggtaga gccatcaggg
     2521 agaattgtgg gataattgcc ataaatcgga agcggcgtaa catttttcgt aacaatatcc
     2581 agcaggtaga tttggcgggc aagcccctga ctaaaaatta cctggcgttg atcggctgaa
     2641 tatgaagggt aaagtatatc gtcagtggtg ctataaatct tttggggcaa accacccaca
     2701 attggaatct gataaattcc gtcccagaga tcgccatcta ttcgcccatt gaaaattagg
     2761 tctaatccat caggggaaaa gcgtggcacg atcaggttat tggcaatcag gtttttaaag
     2821 agcactttag gactctgaaa aatgaaaacc ggctcgtcat ttgccttttg gcgttcgcag
     2881 gtcaaaaagg tcaacgccaa taaacttaag ataaagagtt ttactgtttt catattctca
     2941 tcttgttgga gcgcatacta ttctattcac ctcagaagag tttaaacagt aaaataatgg
     3001 gattcaataa aaatattcac cattaaaaaa taataaattc caatacttaa aatactgtcc
     3061 cgaatttgga ttgaacggtt aaatgaattt ataatttgag aaattcaaga tatcaacctg
     3121 tgataaaaag gtaacttcct gttcttactt tgagtaaatc tctaaaaagt cgaaattgcg
     3181 gccaattcgg tcgccagctg ttcgtccacc atacaaatta tttttttatt aaaaaatgag
     3241 accggttatg tatattatcc tcgtgaaacg ttcatttttt aagaaaatag gccgattatg
     3301 ttggttttcg accggttgga ttggagcacg gtatatttat cgtgaactaa tctttccctt
     3361 tttattttct atcggagttg tgacttttat tttattggcc aattttttaa ttaaatcggt
     3421 tgaccgactg ctgggaaaag gtttgccatt cggtgtcatt tttgaatttc tttatctgaa
     3481 cttagcctgg atagtagcga tgtcagttcc gatggcggtt ttagtagcga ccttaatggc
     3541 ttatggtcgt ctggcggagg ataatgaaat caccgccatg cgttccagcg gcataagttt
     3601 tacgacgatt ctcgtaccgg gatttatttt tggtctggtg gtatcagtag tgatgctgta
     3661 tttccatgat ggtgttctgc cagaatttaa tcaccgagcg cgtctcctca ccagtgatat
     3721 ttaccgcaaa aggcctgatc tgaacattga accagggtac ttcattgacg atctacccga
     3781 ttattccata tacgttaaag aaaaagtcgg cgagagtctg cgtaaagtca ctatttataa
     3841 taaggatgcc cgagacattc agaccacgat ctatgccgat agcggataca ttggagtaga
     3901 aggcaacaca attctattta cccttttctc cggtcaaatt catgaaatcg atttgaaagg
     3961 actatcggat taccgtcgaa ttgattttga gcgtcatcga attgctattc cggttgataa
     4021 tttaatgctc gaacgacgcg aaagtgagcg gcgcggcgat cgagagatga cgatcaaaat
     4081 gatgcgcacc gaggttgagc gttattccag agaatatcaa caagtcattg ccaaaattaa
     4141 caatttagtc cgcaaggagc tgggtgatac cctgacttct tttgatttga aaaagatcga
     4201 gaaaaaagtt caaactcttc aggcggaaaa tctgaagaat ctaccgcccg ccgaagccgc
     4261 ccgccgcaat cagaagttga ctatttttct aaaccgcatt cgaagtgaaa gcaatctggc
     4321 tttcaattat aaaaaacaga ttgccaaata taatgtcgaa attcaaaaga aatattcgat
     4381 tccgtttgcc tgcattgttt ttgtcctgct gggtgcgccg ctgggcgtta ttacccggcg
     4441 gggtggcata accgtctccg ccgttttgag tattttcttc tttctattgt actatgtatt
     4501 cctgatcgga ggcgaagaat tggctgatgc ggctattatt tcacctttct gggcgatgtg
     4561 gacgccgaac ttgatcctat taatcgttgg tatatatctg atctattata cttccagtga
     4621 gcaacgcgtg atagattttt cacggttccc gtatcttttt cgccgtttga ataaaaaaat
     4681 taaaaaaaat ccataaataa gcctggagaa aagtaatgcc gtctgtcaaa caaatgaaac
     4741 gaaccaaaaa ttttattatg gtggatctga tagaaaagaa aaaagctgga ttggaacata
     4801 gcaaagagga aattcaatat ttgatcgcgt cctataccaa aggtaaaata cccgattacc
     4861 agatggcggc ttggctgatg gcggttaatt ttcaaggaat gaccgacgcc gagacttttg
     4921 cgttagtgga aacgatgtta ctttccggta cgaaactcga tttgagtcat attaaacatt
     4981 gcaaggtgga caaacacagt accggaggtg tgggagacaa agtaagcttg attctggcgc
     5041 ctctggcagt cgccgcgggg tgcgttgtgc caatgatttc cggacgcgga ctcggtcata
     5101 ctggtggtac tttggataag ctagaaagca ttccgggttt caacgtaaat ctgagcagtc
     5161 aggaatttgt ccgcttagtc gagaaatatg gtctggctat gggcggccaa accgaggaac
     5221 tcgtccccgc cgaccgtaaa atctatgctc ttagagatgt aactgcgacg gtcaattcca
     5281 ttccactcat ttgtggcagt attttaagca agaaaatcgc tgaaggtatt gacggcttgg
     5341 tgctggatgt caaaaccggt aacggcgctt tcttagccga ctacaaccag agtcagcaat
     5401 tagccaaaca gctgaaacgg gtcggggaaa atttcggttt gcaaatcaaa gcggttatca
     5461 ccgatatgaa ccagccgtta gggcgagcag tcggcaattg gctggaggtt gtcgaatcaa
     5521 tcgcaacgat caaaggtcag ggtccggaag atttaaccac aattacgctc gatctaactg
     5581 cttggatggt cgttctgtct ggaatagaaa agaatttcgg cgaggctcgc gaactactaa
     5641 aacagctgat tatttcgggc gaggcttatc aaaaattctt actaatggta gaagcacagg
     5701 gtggcgatat cagctacatc gaacatccgg agaaatataa gcaagccgcc tggcaggcgc
     5761 cggttctggc gaccgaatcg ggttgggtga aaaacattga cacgaaacgg attggtatgt
     5821 gcgctgtggc attgggagcc ggtcggcaat cgctggactc cgtcatcaaa ccggaagtcg
     5881 ggtttatcat taataaaaaa attggcgatt atgttgaagt tggggaaccg cttgtaaccg
     5941 ttcatgccgg cagtcgcagg gctttccaga atgttcagag cgccatccgt aaagcctatc
     6001 atttttccaa ggcaccggtg acgccgccac ctttatttta tgaaactttt taatttggat
     6061 tttagaaaag cggttaatta tttctttttt taattaaatt tacccttgtt aactgaacca
     6121 gttagtcagg ttttgttaga aagtcgaaat tttaatgaat tatccaaaat ctaaacgctc
     6181 gtccggaatt ttaattgccg ccgaagggct ggacggttcc ggcaaatcaa ctcagctcta
     6241 tttagttaaa cgctggctcg aactcagtgg tcaccatgtt tatttgacgg aatggagctc
     6301 gtcggacatg gttcggcagg caatcaagcg tggtaagaaa aagaacctct tgacaccgac
     6361 cacatttagc ttagtgcacg ccgtagattt tgccgaccgc tatcatcgcc aaattcagcc
     6421 gcttctgaaa actggcttca ttactttatc tgaccgttat attttcactg cgtttgcgcg
     6481 tgatgcggtg cgtgggtgtt ccagcgcctg gttgcgcaac ctctatagtt ttgcgaaaat
     6541 ccccgacatt actttttact ttaaattgtc tccccaaatc gcggctgagc gcattctctc
     6601 caaccgccca aagattaaat attatgaagc cggtatggat ttgaatctgc atcccgaagt
     6661 ctatgatagc ttccgaattt accagggcaa aatcggtacc gaatacgacc agatgagcca
     6721 ggaatttgga ttcgtcgaga tcgatgctac tcaaccgatt aagacccaac aggaaaaaat
     6781 tcgggcaatt attgccgaga aaattgatct ggcgtcgtat aaaaaggggg aactggaatg
     6841 aaaagctttt acctcgaacc gctcccaaaa tgtgacatca gtagactcag tggtaaatta
     6901 atcgtcttgg aaggttcaga cggctccggc cgtacgactc agatcactcg cattaaatcc
     6961 tggctggaag gcaaaggcta tgcggtcgtg aactgcgaac tggctcgctc gcaattggtt
     7021 ggtccggaac tggagttggc tcgccgcggc aataccctct gccccacaac cctttctcta
     7081 ttttatgcta ccgattttat ggatcagctc gaaaatgtca tcataccggc gttacgtgct
     7141 ggcttcattg ttctcgccga ccgttacttt tataccctaa tggcgcgcga tctggtgcgc
     7201 ggcactgacc ttaaatgggt ccagaatatt tacggtgtgg ctgtagtccc tgacctggtt
     7261 ttttacctga atgtctctcc gcaccgccta atcgagcgcc gctggcaaaa agccaacgca
     7321 atggactatt gggagtccgg gatggatatg ggcttatcgc gcgactgggc ggtcagtttc
     7381 ttgatttacc agcagtactt acggaaggtt tttctcaaat tgcaggatat gtatggtttt
     7441 atcgtcatca atggcaataa atcaattaca gccatcacta atgatatcgt aaagcaaatc
     7501 gaaaaagttt taatctaaga gtaattccac cagaaatttt atttgacaat tccataagat
     7561 atttttatat ttcatctact tatgagattt gttatgaatt atttagagct atttcatcgt
     7621 ctccatcatc attccagaat aggaaccggg atgagataga ttatttacag gtattgttaa
     7681 tcaacccggt ccttctgtaa gcgtaccggg tttttnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnttatt tacaggtatt gttaatcaac ccggtccttc tgtaagcgta
     7861 ccgggttttt tattttcagg agcaattatg attactttag ctattcaaaa aacaggacgg
     7921 ttaaacgagc acaccaccaa attgctgagc gaatgtggac tcttcgtcag caatggtagt
     7981 acggcacggc taatgaccga agcgtctaat tttccaatgc gaattctttt cctacgcgat
     8041 gacgatatac cggaatgtgt tgctgacggt cttgctgact taggaatcgt tggggaaaat
     8101 gtctatcatg aaaaagcgcg tccgttgaaa attgtcgagc gactgggttt tgccaagtgt
     8161 cgtctttcaa ttgcggtgcc gaaagacttt agttacgaaa aattgacgga cttgaatggg
     8221 ctgagcatcg ccacttcata tccgaacatt ttgaagcgat tcctgaacga aaataacctt
     8281 aaggccgata ttcatgaaat cagcggctcg gtcgaaatcg cgcccggtat cggactggct
     8341 tcggcgattt tcgatatcgt cagcaccggt agtactctac tgaccaacgg gctgaaagaa
     8401 gttgtgactg ttttaaaaag tgaagccgta ctggtagccg ctgaaaattt aagtccggaa
     8461 aaaaccgatt tgctggaaca attacttctc agaatccggg ctgtccaagc cgcgcatacc
     8521 cgcaaatata ttcttctcaa tgccccagat tccgccatcg caaaaatctg cgccatttta
     8581 ccgggaatga agagccctac gattatgccg ctggctgagc ctggttggag ttcgctccat
     8641 tcggtgatcg aggaacatca gttctgggaa cgtatttccg ccttaaaagc ggctggcgcg
     8701 cagggcattc tggttattcc aattgaaaaa atgataatgt aggatgccat tatgaaagtt
     8761 ataaaatatc ctcaaaaaac ttcctggcca gagttagttc gccgaccagc aattgataat
     8821 aaaatcttac gaaatttagt gattgaaatt atagaagcgg ttaaaaccga cggcgatgac
     8881 gccctccggc aattttcctg gaaatacgat aatgtgcgtt tgaaagaaat cgagatcctg
     8941 cgcgatgaat tggcatctgt cccgaatgaa gtagatgatg acctccggaa agccatcgag
     9001 attgcgcgtg ctaatattac caaatttcat tcctctcagt ttgaaggaat tgaaaaggtt
     9061 gagactactc ctggcgttat ctgctggcgt aaaagtgtgc cgatcgaaag tgtcggattg
     9121 tatattcccg gtggatcggc gccgctcttt tcgacggtgc tgatgctagg tattccggcg
     9181 cggctggctg gttgtcccga aattattttg tgcacaccgc ccaataaaga cggtcgcatt
     9241 catccggtga ttctttacac agcccaaatt ttgggcatcg agcgaatttt caagatcggc
     9301 ggagctcagg caatcgcggc gatggcttac ggaactcaga gcgtaccgcg tgtcgccaaa
     9361 atcttcggac caggcaatca atacgtgaca atcgccaagc aaatcgtcag cgctcagggc
     9421 gtagcgattg atatgccagc tgggccttcg gaagtggcgg tcgtagccga ccggttcgct
     9481 aatccggatt tcgtggcggc tgatttgctt tcgcaagccg aacatggcgc cgatagtcag
     9541 gtcatttgtg taaccgataa cgaagaatta attccgcaag ttatcacggc ggtcaagcaa
     9601 cagctggcgg aattgccgcg ccgcactatc atcgaaaaat cactggaaag tagtcgcctg
     9661 attttggtca ataatctgga tgaagcatta gaattcatca attgttatgc accggaacat
     9721 cttattcttc aaacccaaaa tcccgagcag tgggcggata aagtcattaa cgccggttcg
     9781 gtttttctgg gtgattttac tcccgaatca gccggcgatt atgcttccgg tactaatcat
     9841 accttgccta ctaacggtta cgctcgcaat tatagcggaa tttcggtgga cagctttgtc
     9901 aagaaaatca cttttcagca gatttcgcct gccgggctaa aagccatcgc tccggctatt
     9961 gattgtatgg ctaccactga aaagttgcag gcgcatcgca acgcagtccg aattcgcctg
    10021 gaagcgattg aaaaaggagc tggacgtggt tgatctggaa aaactggcgc ggcgcaatat
    10081 cattaacctc cagccttatg caaccgcccg cgacgattat agcggcgcga tcggcatatt
    10141 tctcgacgcc aatgaaaatt ctctcggtag cgtaatccca caaaaattca atcgctatcc
    10201 cgatccgcat caaaaacgcc taaaacagga aattgccgcc cttaaaaatg ttaacccgga
    10261 acaagttttt ctgggcaacg gcagtgacga agtcattgat ctattgatcc gcgttttctg
    10321 tcaaccggga acggaaaaaa ttatgatcct actgccaacc tatggtatgt atgccgtcag
    10381 cgccgccagt aatgatgtcg gtgttgtttc cgttgcttta actccgaact ttcagattaa
    10441 tctaccgcaa atttttgagc aaattgcggg cgtcaagctg atttttctct gttcgccgaa
    10501 caatccgact ggtaattgcc ttaatccagc ggatgttgtc aatttgctgg agaaattcaa
    10561 gggaatcgtc ataattgacg aagcctacat agacttcgca ccccaaaaaa gctttctgcc
    10621 aagactgaag gagttcaata atctggtaat tatgcaaact ttttcgaaag cctggggctt
    10681 ggcgaatctc cgactcggga tggctttcgc cgatcctcag attattgatt ggctgtcgcg
    10741 actcaaatat ccctataatg tcaacggctt gacccaggag ctggcgttgt cggctttaga
    10801 acgatctgcg gaaaaggatc ggatggttac cgaacttcgc accgagcgcg aacggcttgc
    10861 cgattcactc aaagaattgc cgatagttga aaaggtttac ccttccgacg ctaattttct
    10921 gctggtgaaa tttcgggatg cgcgtagcgt gtttcaatat ttaattaatc aaaaaatcat
    10981 cgtgcgtgac cgctcgcaag cctggcattg tgaaaattgc ctgcgaatta ccatcggtac
    11041 tcctaacgaa aatgatattt taattgctaa actcaaggat tttgcaagtt gaaaaaagta
    11101 ctttttattg accgcgacgg gacattgatt atcgaaccac cggatgaaca ggtggattca
    11161 ctggagaaac tcgaattcct tccgggcgcc atcacaaata tgaacaaaat cgccacccaa
    11221 tttgattacc aactagtaat ggttaccaat caggatggac tcggaacgga tttatttcca
    11281 gaaaaagatt tctggccagc tcacaataaa atgttacaaa ttctggccgg cgaaggcgtg
    11341 gtattcgctg agattttgat tgatcgtagc cttccaaatg acaatgcccc atcgcgcaaa
    11401 cctggcatcg gcatgttaac agattacctt aagggcgatt acgatctcga aaattcattt
    11461 gtaattggcg accgggagac tgacgtacaa ctggcacgaa atctcggcac aaaggcgatt
    11521 taccttggaa gcaacgaatc tatttcagcc gattataagg ctaaaaactg gtcggaaatt
    11581 tataattatt taagcaatat tagtcgcaca gccaccgtca cccgcaaaac taacgaaact
    11641 gacatccgcc tggtaattaa tctgaatggc aaaggtgcaa atcgaataga caccggtatt
    11701 ggttttcttg atcatatgct gaatctattc gctacgcatt ccggttgcga tcttgatttg
    11761 actgccagag gcgatctcgc agttgatgaa catcatacgg tcgaggacgt tgcccttgtt
    11821 ctcggcgaag gtttgctaaa agccctcggt gataagaaag gaatcgaacg ttacggtttt
    11881 cttctgccga tggacgagtc actggcgcag gtggcactgg atttttccgg acgacgtgaa
    11941 ttcgtctggg aatgtgactt caaaagggaa tatatcggaa aaatgcccac tgaattattc
    12001 cgtcactttt tcaagtcctt tgccgattcg gccgcctgca ctctgcatat taaagccacc
    12061 ggtgaaaatg aacatcataa aatcgaagca gttttcaaag ccgtcggtcg ggcagtgaaa
    12121 atggctattc gtcgcgatcc aacctgcgac cagattccga gtagcaaagg caaattatga
    12181 aagtggtcat aattggctat aattccggaa acattcactc ggtaagactg gcacttcagc
    12241 gtctaaatgt caaagccgtt gtcagttccg acccggccgt gattcaagcc gcggataaag
    12301 tcattttccc cggggttggt gaagcctctt ccgcaatgga atctatcaag gcgaatggta
    12361 ttttcgagat attgccacgg ttgcagaaac cgaccctggg tatttgtctc ggtctgcagt
    12421 tgatgtgccg ggattcagaa gagaatgcta ctcaatgttt tgggattttc gacattgctg
    12481 taagaaaatt tccaccgctc gccaaagtgc ctcaggttgg ttggaataca cttttcgatt
    12541 taaaaggtga tctttttcgc gatgttgctg aaaattccta tgtttacttt gttcatagtt
    12601 attatgccga attgagtgaa cataccgttg cgcagaccga gtatatcatt ccctattcag
    12661 ccgctatgca gaaggacaat ttcttcgcag tgcagtttca tccagagaaa agcggtaaag
    12721 ttggtgagca aatcctgcga aatttcatta aactatgatt acaatcatac ccgcaattga
    12781 tattataaat ggcaagtgcg tgcgccttag ccgcggcgat ttcaatactc aaaaaattta
    12841 taacgagaac ccattggaaa ttgctcgaca attcgccgat gccggtatca ggcgtttaca
    12901 tctggtagat ctggacggcg cgcgccagaa gcgagtagtc aactgggaag tactggaaaa
    12961 gattgcaacg gggacgcctt tgcaaattga tttcagtggc gggattcgga gcgaaacgga
    13021 tgtgcggatt gctttcgaga gcggcgcacg gcaaattact gccggaagca tcgcaatctc
    13081 cgcacctgat ttagtggaag aatggttgaa aaaattcggc ggcgaaaaaa tcattctcgg
    13141 cgccgacgtt atcgatcgaa aaattgccat ccacggttgg caagaaaaag ccccactgga
    13201 aataatggaa tttttacaga attttcaaga aaaaggcgtt caacaagcga tttgcaccga
    13261 agtcgcacgc gatggaatgt tgactggacc ggcttttgaa ttatatcgag aaatcaaaca
    13321 aaaatttccc gatctttact tgattgccag cggtgggatt agccgaattg acgatattta
    13381 tcagttacaa gatgatggaa ttgacggcgt tattattggc aaagcgcttt atgaaggata
    13441 cctaaaattg agtgagttga aaggattttt atgttagcta aacgcattat tccctgcctc
    13501 gacgtcaaag atggacaaac agtcaaaggc gtaaatttta ttaatattcg gcgtgccgga
    13561 gatccggtag aactcggcgc ggcttatgcc gagcaaggcg ctgacgaatt ggtttttctt
    13621 gatatcacgg cttcaatcga aaaacgcgga acatttatcg aattggtgcg cgaaattgcg
    13681 aaacatttga acattccgtt taccgttggc ggcggagtac gcaacgccga tgacgtcgct
    13741 cgcctgctgg acgccggcgc cgacaaaatt tcaatcaata ccgcggcggt caatcacccg
    13801 gaaatgatcg aaaaattagc cgggcgcttc ggcagtcaat gtatcgtaat cgcgattgat
    13861 accaaacata ccgaaaacgg cgacttcgta tatattaatg gcggaaggac cccaactaac
    13921 caaaccacgc aagcctgggc acggcaagtc gtcgagctcg gcgctggtga aattctacta
    13981 acttcgatgg atcacgatgg caccaaaagg ggctttgcgc tggaaattac gcgtgaactt
    14041 gcggaaaatt taccgattcc ggtaatcgcg tctggcgggg cgggcacaat ggaacatttt
    14101 tgggaagtgt ttaaaatagg aaaagccgat gcggcgctag cggctagtgt ctttcacttc
    14161 ggcgaaatcc ccattccgga attaaaaaaa ttccttaaat ccaatggtat cgaggtgcga
    14221 ttatgaacat tgattttact aaaaataatg gcctgttacc ggcaattatt caggatgaaa
    14281 agactggaaa agttttaatg ctgggttata tgaatcgaga atcttatgaa aaaacaaagg
    14341 cgaccggcag ggttactttt tttagtcgga gtcggcaggc actctggacg aaaggcgaaa
    14401 cgtccggaaa ttttttagaa gtcaaggaga ttctaagcga ttgcgacggc gacacgcttt
    14461 tgatcaaagt taaaccaacc ggaccaactt gccataccgg cgccgatacc tgtttcaatg
    14521 aaaaaaacat agccgagaag ccgttcattt tcgaactgga ggagattatt cgcgatcgcc
    14581 gcaataatcc gaaaccaggc tcttacacta ataagctatt cgatgccgga gacgaacgca
    14641 tcgctcagaa agttggcgaa gaagccaccg aagtcgtcat tgatgccgtg accggtaatg
    14701 aaatgcgcct gccagaggaa gttgccgact tactatatca tttgcttgtg ctattagtag
    14761 cgaaagatgt cgcgctcgac gatattatga atgtgctgga aaaacggcat agaaaataaa
    14821 caattaacct ttagaagttt tccaatggat acaacccgac tagccgttta ctttggcttt
    14881 actactaaag cgacagtcac tcgaaatagc atttttctat ttattcagac atcctgattt
    14941 ctttccagaa aagtggattc tacattcctg cagataaata ctaaatctca ttctaattat
    15001 agctaattaa ttctaaaaat tccgcttcgt tgagaatttt gacgcccagt tgccgggcgg
    15061 tttcggcttt cgagccggca ttttcgccga cgactacgta gtcggttttt ttactgacgg
    15121 aggaggcaga tttgccaccg tatttttcta cgagtgcttg ggcttcttcg cgggtaaaat
    15181 tggtcagcgt gccggtgaaa acgaaagtct ttccggcaat ttcttctttt tgtttgtaat
    15241 cctgttcgct actgatccga ataccactgc ttagtaattt ctcaattgtt tgacggttgg
    15301 attccgcatt gaaaaaattg cggacgctcc gggcgacgat tggtccaacg ccttcgattg
    15361 ccattaattc ttcttcagaa gcctgactga ggcgttcgag gctaccaaat gcttttgcca
    15421 gcactttggc catatgttcg ccaacgttcc gaatgccgag tgcatagatg aagcgcgcca
    15481 gactaacatt ttggctggcg gtgatggcat ccagtaaatt ttgggcagat ttttcgccca
    15541 tccgttccag ggtcaataat tgttcttttc gcagagaata aatgtcggca acatttttta
    15601 ttaaaccgct atcaaccaat tgttcaatca atttctcgcc aaggccttca atattcatag
    15661 cccgtttgct agcaaaatgt tcgatgctgg ctttgatctg agccggacaa ttaatatttg
    15721 gacaacggtg tttagcttca tcctcctcgc gtacaaccgg cgttccgcag accgggcaat
    15781 tggctggaat cgaataacgc ttttcgttgc cggtccgtcg ctcagtgatg actttaacca
    15841 cttgcggaat gacatcgcct gcccgctgaa ctacgaccca atcaccaatg cgaatatcct
    15901 tgcggtcaat ttcatcctga ttatgaagag ttgcatggga tacggttacg ccgccaattt
    15961 gcaccggctt taaatgggct accggcgtaa tggcacccgt ccgtccgacg ctggcttcga
    16021 tgtcaattat ctgagttact tcctgctgag ctttaaattt tccggcaata gcccagcgag
    16081 gactgcgtga ttttattccg agtttagcct gctgagctag agaatcaact ttatatacga
    16141 caccatcaat gtcataaggc atcgagtcgc ggatggattc aattttatga taatacgcta
    16201 taagcgcctc gacttcttta gctatttcaa ttaacggatt gacccgaaag ccccatttga
    16261 gtaaagcttc cagaaattca tgatgggttt gaaaaaaata gccttctaaa gatcccagtc
    16321 cgtaacagaa tattttcaat gggcgtttag cggtgatctg cggatcgagt tgtcttaccg
    16381 agccagccgc cgaattgcgg ggattagcaa atagcggttt tccttccttc aattgttgtt
    16441 cgttcagacg ctgaaaatca gctttatcca tataaacttc gccgcgcact tccagtagag
    16501 tcggaatcgg caaattcggc attaatctga gcggaatact gcggatcgtt tttagatttt
    16561 gggtaatatc ttcgccggtt ataccgtctc cacgagtaga acccgaaatg aaaacaccat
    16621 tctcataaac gagttcgact gccaaaccgt caatttttgg ttcgcatacg aactggacgg
    16681 tctcacccgg caaacttttt tgcacccgaa ttgcaaaatc gcggatttcg ccctcgtcca
    16741 ttgcattgtc cagactgagc ataggaacgc gatgggtaac ggttttgaac tcgtctaaag
    16801 gttgggcgcc gacgcgttga gtgggagaat ccggcgttct caattccgga taagccgcct
    16861 ctaactgttc tagttcacgc attaggcgat catattcagc atcgctaatt gtgggattat
    16921 caagaacgta gtaattgtaa ttagcagtct ctatttgacg gcggagttcc ttagcctttt
    16981 cccgtagtgc atccaaattc ataaataaat ttactgcctc ctatttaaaa atggtatgag
    17041 aattcacaac gattttttat caaactataa tggagtaaaa aataaattgg gagtgtccta
    17101 aagtattttg gcaaatgccg gtaaaaatga tgattgagtt acggagagcc gcttgccagt
    17161 gtgttctggg gatcaatctt tgggaatgag ttctccacaa accggacaaa ttagataaac
    17221 attcggttca ccgaaatagc gtaaattggt gatgcgaaaa ttcacctgcc cggacatatt
    17281 cggacgctgg gtaataatta tactgcccga agcagtattt tgtggtttaa gcgggatatc
    17341 gctattcagc acaatcacaa tttcctgttt ttcaataatg ttgccgtatt tatcgtaaat
    17401 atctataaat ccagctaatt cgctaatgga tttcatcgaa gtatttttta cctcgaccct
    17461 aatttcaatt aagttattgt ccagatcgga aattattata tccgaaacct cgacggcttg
    17521 ctgggcaaat gcgattgtgc taaacatcca gatcagaaat atttttttca tggattgcat
    17581 cgcttacctc ttttattaca atgcaatata cattaaatca attttatagt caagttatca
    17641 gttagggagt tttaaagtga tcggcttcgg gtaaaatatt gtttaaatat tttcgtaaaa
    17701 ttaattttct aacactatga aaatttctta tttttttgta gtttacttaa tggtaggcaa
    17761 aacaattcaa tttctcaaaa gcgccaagaa taaaggccgg aaacaaaaat gctcctgcca
    17821 ggttgtttca tcggctttta gtccagaaaa agctatttac ggcgttgata ccgaattcga
    17881 caagcttagt cgaatgggaa ttataccgga agtcttacct attaattatt ccccggttac
    17941 gatacaccgg aagccattgt tatcgatata tttataccaa aatatatgag gttgtctatg
    18001 aatgaaaaaa tcgcacgcga aggcttaacc ttcgatgatg ttcttctaat accatctgaa
    18061 tcaaacgtac tgccgaaaga ggtcagtctc gacgccaagc tgaccaaaaa aattcaatta
    18121 aacataccaa tacttagtgc cgctatggat actgtgacgg aagggaaaat ggctatcgcc
    18181 attgctcgcg aaggcgggat cggtattctt cataaaaata tgagtatcga acaacaagta
    18241 gccgaagttg acaaggtgaa acgcagtgaa agcggtatga ttgtcaaacc aatcacacta
    18301 tcctccgata gtacaattcg ggatgctcta aatctaatga accgttattc aatttcgggg
    18361 attccggtgg tagataacgg tcaattaaaa ggtattctga ccaaccgtga cattcgtttc
    18421 gagaccaacc ttgatctgcg catttcggaa agaatgacta aggagaattt agttaccgca
    18481 ccggaaggca cgacactcga agaagccgaa gaaattcttc agcgtcaccg cattgagaag
    18541 ctcttaattg tggacgataa aggacggctt aaaggattaa taacggttaa agatattctg
    18601 aagaagaaac gttttccgaa tgccgccaag gatgaatttg ggcgtctgcg ggtcggagcc
    18661 gcagttggtg tcggttccga ttacctctta cgtgccgaaa aattagtcag tgaacaagtt
    18721 gatgtgctgg tgatcgacac tgctcacgga cattctgcca atgtcatcaa aagtgtacgc
    18781 gagttgaaac gccgctttcc ccaggtagac gtaattgccg gcaatgtcgc gactgcggcc
    18841 gctaccaaag ccttaataga cgccggagcc gatgcggtta aagtcggcat tggtcccggt
    18901 gccatttgca caacgcgggt gatcgccggg gtgggaattc cacaattgac agccattttg
    18961 gattgcgccg aagttgcgat gaaaagcggg attccgatca tcgcggatgg tgggattcgt
    19021 tactcgggcg atattgccaa agccttagcg gcaggagcca gtaccgttat gcttggttca
    19081 cttttagccg gtttggacga aagtccaggt gaaacggtat tatacgaagg tcgtgtttat
    19141 aaatcttacc gcggtatggg atctattgcg gcgatgactc atggcagtag cgaccgatat
    19201 tttcaggagg gtgaagaaac cagcaagtta gttccggaag gcattgaagg cattgtacca
    19261 tataaaggct ctttagcaga tacaattcat caactaatcg gcggtctgcg ttcagcaatg
    19321 ggttattgcg gcactgcaac aataaatgaa ttacagaaac ggggatgttt tattcgtgtc
    19381 acgcaggcat ccattcagga aagccatcca cataatgtta aaatcagtaa agaagccccg
    19441 aattaccgct tggaaaatta aagagggcaa taattatact actgacgttc aggacagatt
    19501 agctaaatac tcagccagtg ccgatttacg gtgagcggca ttattcttat ggataatacc
    19561 tttccgagcg accttgtcta tcagtgccac tgcggctttg aaaaggggtt cagcttcagc
    19621 cttagtttta gtatttttaa cctttttaat cgaggttttc atcattgaga catagtgccg
    19681 atttcttaag gcggcttttt gagcctgacg agttcgtttt aaaacagacg cattcttttt
    19741 cattaaactc ctttcacaaa gattaccaaa aagtaggcaa atttatttat attgaagctc
    19801 attttcaaat aaaaaacctc cgaaacgaat gatgattatt ctaaaaatac ttctcgtctt
    19861 attttttatt ttgattttcc tcctttttgt aactctattt tcgaaaacct ggctgagtat
    19921 ttttgggaat ttcagcgaac aaactaaaac tggcacgctg aaagttcagt tagccggtaa
    19981 attgagtagc attatagttg tgatggaaac gcccgaggta aattttcgta ttggtatcct
    20041 cggcttgcat ttcaaagttg ggcaaaaaaa agccccctca ctagccaaga aaaaatcacc
    20101 cgaacccaaa ccagagattc ccaaaaagcc ccgaaccagg cggtctaaat tctcaatatt
    20161 tgactggatt gagtttggga aagcactgac actacgattt tttaaattgg tccatattga
    20221 gacattaagc gcaaatctta ccgtcggttt gggaaatccg gcctataccg gtcttttaat
    20281 gggcgcttat tacacgattc gagaaacagt gaaagatttc caatcagtgc atatcgtccc
    20341 agattttgtc gccaaaaaat tcaccggtaa agtcgaattc cgggcatcga ttagattgat
    20401 aaaaacaatt cctatactta tttttatagt gaattttttg agaaaaatga aaaataaaaa
    20461 acggaggtaa attatgctgg aaggtttagt agacaccctg ttaagtcgtt tacgtgaaat
    20521 tgtagcttct gaaacagtaa ttgggaaacc aatcgaaact ggagatacga tggtaatccc
    20581 tattacgaaa atttctctcg gttttatggt gggcagtagc ggtaatccag aagaacaaaa
    20641 aaagaagact gagggcaccg gcttaggagg cggcgctttg atcgaaccgc ttgcagtcat
    20701 tacaatccag aagggtgagg ttaaaattca ccgactgaaa gagaaagtat caggcgttga
    20761 caagcttatg gaaatcgttc ctgaagtgct ggaaaagtat attaaaccca aatgcgagaa
    20821 aaaatcagcc aaagaaaata cgtgattact tatttcatcg aaaattgttg aaaagttttt
    20881 cttggttttt tgccggagtg aacctatttt cttagcgctt tatatatgag agtagtgata
    20941 tggaaataga tatattaaaa aatattccat tatttgccga tcttaaggat gatattatcc
    21001 agcaaatcta taaccttatg caaaagcggg catataaaaa aggcaacatt atcctgatgg
    21061 aagaggaatt cggtgatacg ctctttattc tcagtaaagg aagtgtcaaa attactagaa
    21121 tgggtgagga tggccgtgag gtaattcttt cgatactcgg tgaaggtgat tttttcggtg
    21181 aaatgtcaat tctggatggc gaaaatcggt cagctaatgt tgtcgcgctg gaagacaccg
    21241 cagttttaat tctaaaacgg ggcgattttt tggatttgct ggaaaaacat ccacagatag
    21301 ccatcgcttt actccaggaa atggctacgc gtctgcgccg tagtgatcag caaattgaag
    21361 gactttcgct aagtgatgct gagaaccgaa ttgcaatgtg cctcctgcgc ttagcggaag
    21421 atctgggcgt tatcaaacag ggaaaggtca taatagaaaa cctgccctat cagcaggata
    21481 ttgctaatat ggctggtact tcccgcgaaa ctgtttcacg aatgttgact ctactacaac
    21541 gtaaaggtta tatccagaaa tatggtcgtc gtctgattat aaacaactac gacggattcg
    21601 ttaaacaatt cagctgaagc acgacctcct taagtacgtg ctgactacac aattttaggt
    21661 taattcaaag aaaaatattt tatttataaa aataacaatt agatacttca cccataattt
    21721 gataataaat aaatacaaat ctctcgaacc ctgctaattt taccgataaa atagacattc
    21781 tctattatat gaggattagt aataatctat gactttcacg tgtaggatat ttatatttac
    21841 accgcatttg aaaaaaatta tgagtattta ttaagtttac cattaacaat ggcgcgaatc
    21901 aattctacca atgccgaaaa agaaatggga tttctggatc acctcgaaga actgcgttgg
    21961 cggatcatca aaattttgat tgcactagta ttaggcaccg taggggcttt cctattttct
    22021 gatcaagcaa ttgacatttt gatgcgtccg gcgcggcaag ttggctccga gatgactatt
    22081 caggttctca aaattcaagg cttattgatg ctgaaattca aggttgcctt tctactcggt
    22141 ttggtaattg ctctaccgat agttgcctat caattctggg cgtttatctc accaggtctt
    22201 ctgcaaaatg aaaaacgttg gggcccggga ttaataatcg gagtaaccgt attctttcta
    22261 ttaggcgcaa tttttgcctt ttttgttcta attccgatag ctctcaaatt tctgattggg
    22321 attggtgtag ctggtgttga acgcaacatt tcgatcgaat attacacgaa attcgtcatg
    22381 cagttgatac tcgcaactgg attgattttc cagatgccag ttttgtcttt catcctcacc
    22441 aaaatcgggc taatcacgcc gcaatttctg cgcaaatcgt ggcgttatgc catcattatt
    22501 ttaatggtta ttgccgctat tattactcca cccgatccag ttagtatgat ttttatgacc
    22561 ctccctttat tatttctata cgaaatcagc atctggattt cgcgcatcgc cagtcgcccg
    22621 aaaattcaaa atgagaccga tcaaatagaa ttggctggat agttcataat gccgcctaaa
    22681 gatttaaata acatatttgc accaatttcc actgagctcg atctattcca aaaagaattc
    22741 agacaagttt tatattccga agtggaatta atcaaccaga tcggcagtta tatattatca
    22801 ggaggcggca aatatattcg gccggcattg gttctgctga gcgccaatct ctgtggtaaa
    22861 tcgaacgaac attcgatcat cgccgccagt gtcgtcgaga ttctgcatac tgctactctg
    22921 gttcatgacg atattataga cgattcggaa gtccgtcgcg gtcggccttc tgtcaaagcc
    22981 atctggtcga ataaaatcgc cgtattagcc ggggacttcc tttttagtcg agctttaagc
    23041 aatatgttaa ggttaaagga ttttacagtt ctcgaattac tttctcaaac ttccgaattg
    23101 ctcagttccg gtgaattatt acagttggaa aaaagcctga acaacaatat ggatgaggaa
    23161 atatactatc aagtgatctg ggctaagact gcttcacttt ttgccaccgc ttgtcaggcg
    23221 ggcgctctca cggttgccgc caatgaagaa cagaccaagg ctctcttcga ttatggtcgc
    23281 tgtctcggat tggcattcca gatcgaagac gatcttttcg attgcaccgg cacatctatt
    23341 cataccggca aaccggtagc gcgggatatt aagcaaaact tagttactct gccgttaatc
    23401 cacgctttga aaaccttagc gcccgaagaa cgccaccgaa ttcgccagca gttgaaaaat
    23461 cgcccttctc ccgaacagat aaccgcaata acggagctca ttatcaatac caatggcatt
    23521 acttatgccg aaaataaatg ccgggagttg atccagcaag ccgttgcggc acttgctatt
    23581 ttccccgact ctcaatataa atccgcccta attaatctgg ctaactacac gcttatgaga
    23641 aataattgac aacgactact aatcccaatt ctattctgat catgcgcttg agttcgatgg
    23701 gtgacatcgt tgttctcact ccaattttcc gtagtttaag ggagaaatat cctttagccc
    23761 gcattgattt tctgaccaag atggaattcg ctgaaatagt tgccaataat ccttttctat
    23821 caaatatcat cacttacgat tcggggacaa aattagctgg ctggcgagat ttatgccgcc
    23881 aattgaaagc ccaagattac gatttactga ttgatatgca caataatatc cgcagtcata
    23941 ttctttctca ttatttgcgg ggtacttctc ttctgcggta tcataaacct tacctgagac
    24001 gttttctact tttctacttc tggctgaatt ttttctcgca cgatttccgt cttttgaaag
    24061 aatactataa agttttacag cctcttggga ttaactatcc gccgaccgct aagccggaaa
    24121 tttttctcga gccggaagca gttaccaaag cgcaggatat tcttgttaaa tatgaaattt
    24181 ccgagccgtt cattatcatt ttgcccattg caaattggcc aaataaacta tttcctctcg
    24241 aaaaatatat caatgtggcg cgccgaatca acaccgatct acagatgccg gtactctggc
    24301 ttggtggaaa agctgatatt cacctgcaac agcttgatta tttagttcct ggtaagagtg
    24361 ttcgtttagc aggcgaaacc accttaagcg aatctctggc gctgattggt ctcagccggg
    24421 cggtaatcgg taatgatacc ggcttgacct atgcggcgga ggccctggga aaaccagtga
    24481 tattaattct ggggccgacc gcgcgggaaa ccggcgccgg ctgttacgca ccaaaaagta
    24541 ttgtcctgga agctcaacta ttttgtcgtc cttgttctca aaaaggtgat cggcgttgct
    24601 atcgccgcca acggcaatta tgttttaatc aaatcaccga tgcgcaggtt tacgatgccc
    24661 tggtaaaaat aataggagcc gagtcttgac tatattctgg ctggtaattt ataatgctat
    24721 tgttatgccc attgcctatt ctggattgat gattctatca cattttaatt ccaaaattaa
    24781 agaaggactg aaaggacgtc agactctctg gctaaactta cagaaattcc gggaagaatt
    24841 gcctaaacgt acagtctatt tagtccactc ggcatcctta ggtgaatttg agcaagccaa
    24901 accggtgatc cgcggcttga aaaccctgcg tccggatatt ttgctggtag ccagtttcac
    24961 ctcgcccagt ggattcaaaa atgccgaacg aatgccagaa gttgatttat ttacttacct
    25021 tccaattgat actttcttac aaacccgccg ctttttacag aaattacgac cgactaaaat
    25081 tatttttgtg acttacgaat tgtggccgaa tctcattttc catgctaacc gatataaaat
    25141 aaaaacttat ctgatttcag cacgaattcg tccagatagt tccaaactaa agccgatcat
    25201 tcggcatttt ttcgctgacc tttaccgttc gctggattat atttatgcca tttcagaaga
    25261 acataaacaa tcggtgctga agctaatcgg ctcagtcaag attaccgtat tgaacctcgg
    25321 tgacactcgc tatgatcaag ttatcgagcg ctccaaatta ctcgctaata aaatggttac
    25381 gcgagttttt aacgatggat ttgttttcat tggtggtagc atttggcctc aggatgcccg
    25441 tccgcttcta ccagctctct accaatgtct gaagaatttt cctgatttta aaattatctt
    25501 aacgccgcat gaacccaatg actatgcttt ggaaatgctg gaggatgaat ttcagacgca
    25561 taatattgag acagtccgtt attcacaatt gaagggcatg cctgccaatg tacgggttgt
    25621 gcttattgat cgtatcggta tcctggctga actttatcac caggcgaatc tggctttcgt
    25681 tggcggtagt ttccggggct caattcataa cgttatggag ccagcggtag ccggtttgcc
    25741 ggtgctcttt ggaccatcgt atcataattc ccaggaagcc gaattattgg tgcagtccgg
    25801 tggcggactg gtctgccttg atgaaccggg tatttattca accatcaaac atctaataac
    25861 taaaccggac gattaccaga aagccgccca atctgcccgc caggtgattc taaaaaatat
    25921 gggcgcttca tcccgaaccg tccaagaaat cctggagtcg gaatgaaact tcgacttgac
    25981 ggaatagatt tttcctacca cggaaaccaa cctgttttca aaaatctcga cctgacaatt
    26041 cccctaaatt gctgggcagt tttgactggt gactgcggca ctggtaaaac taccctggct
    26101 aaattaatcg ccggtttact caagccggat aaaggctcaa tccgatttga aaatgataat
    26161 ggtctcaata attcattaga agtcggctat attttccaaa acccggatga ccagtttgtc
    26221 cacttcaata ttgaacgaga agttgccttt aatttggaaa atcgcggtgc cagtcccgct
    26281 atcgtccgga aggccgtgca acttgctttg gagagtgtca atctatggcc gcgccgtgag
    26341 aattcaccgc atgaactatc cggtggtgaa aagcagaaac tggccatcgc tgattggtta
    26401 gtaagcaaac cgcaagttct gatttttgat gaaaccaccg cttttctcga cttgccgact
    26461 cgttttgaac tttatgaaaa tattcgccgg ttaatcgtcg ctggactcag cgtcatctgg
    26521 atttctcaag aagattatga aatcgctttc gccgattaca taattgagct attacctggc
    26581 tatttactcc ggacgagttc gaataaataa tcctattttt acaattaaat gaattaccta
    26641 atctgccaga acctcgaatt ccaatatcca caaaatccga acttccgttt atctattccc
    26701 gaattgactt gccctctgaa ttatcctctt ggtgtttacg gtctgaccgg ttccggtaaa
    26761 agcactttcg gcaaaatcct gggcggttta ttaactccca cgaagggatt ttttcaaatc
    26821 gaagcacccg agccaaattt atccagcagt aaaactaaac cgcgcatcct ttatctgccg
    26881 caatttccag agcaaatttt cctcggaata acgatcggca atgccttgca gaaaattctc
    26941 aagagctgga acgacccggc tgaattgacg gctaaattcg ccgataacct tcaaaaatta
    27001 ggattggatt ataacctcat tcgcgaacgc tacggttatg aattgagtag cggtgaactg
    27061 cgattggcta ctattgcttt gggtattacg cttgcgcccc aattaacgat tttcgacgaa
    27121 ccgacgattg ctcttagccc gtccagccgg acgaaatttg aaaacattct cccagaatac
    27181 gattcgcaaa aagaatttat gattatttcg cacgactacc gtctccttcg taaattatgc
    27241 cacaatatat taatttttga taaaggttct gtgatatttg aaggcgaatg gaaaatactg
    27301 gatcaaaatt tttctataat tcacaaggta ggattgaatt tattggaaaa gtcctatcaa
    27361 aggaacagca atgagtaaaa atccaattaa acacactctt caggtcaaac gtaacattac
    27421 ctccaagtac gacgccatcc ctgcccgtaa aaccgtaact atctccaata tcatttcttt
    27481 agttcgagca tttttagcgg caccgattgt ctattccctg cgcaataatg acagcaagac
    27541 cgctcttatt ttcatcctga tcgcgatttt gtccgatatg ttggacggct ggttagcgcg
    27601 ctccagtaat gaaatcactg tgcttggcaa gattctcgat ccatttgccg atagcgtcgt
    27661 gattatgagc gttatgctgt tcttaacagt caaggaacgt atcccgactt tctattttac
    27721 cataatgctc gttcgttatt tccttatttc ggtatttgga atttatatgt tgaataatag
    27781 caaggtttcc ccacagtcaa ataaactggg aaaagtctca gttgttttta ctgtaatcac
    27841 cgtgctggct ttcatctatc ccaatttttt cggctcatcg gctaagccgt tgatgattgt
    27901 ttccataatt tttatggcac tgtctctggt tcaatatatt tatgaatatt ctcggcaaat
    27961 aataatcgct agccgcgaga agaaagccaa cctttctaaa aatgattaat tttggtttaa
    28021 ataaaactgg ccgttttcta gccagtcgcc tacctctatt agggcggttt ttccggcttg
    28081 atgaaaaaac gctggaggaa atcgaagaag ctctaatcca aaccgacctt ggagtagaat
    28141 taaccgaaac attaatcgaa cgcctgcgca aagttgatcg caaagaagcg gcccgcttga
    28201 atgaaatcct gcagaatgag ttgagtgcct taattcagcc tgccgtagcg acgaaaacga
    28261 attttcacaa accgactgta attttaatcg ttggagtcaa tggcaccggc aaaactacca
    28321 caatcggcaa attggccgca aaatatgcga gcgaaggtca gcgcgtattg atttgtgccg
    28381 ccgacacttt ccgagcggcg gcttatgaac agttgcgaat ctgggctgaa agaagcggtg
    28441 tagagttcat aggtaaccca cagggcaaag acccttcagc cattgctttc gatgccgctc
    28501 gcgccgctca agcacgcaat gccgatattc tcctgattga tactgccggt cgcttacaca
    28561 caaagtccaa ccttatggaa gaattagcca aggtcaagcg cgtcattggt aaggtaattc
    28621 ccgatgcccc gcacgaagtc tggctggtac tggatgccac cgtcgggcag aacggaatat
    28681 tgcaagcgcg agaattccag aaagccattg ggataactgg gctaatcctg accaaattgg
    28741 atggcactgc caaaggcgga attgctcttg ccatccatcg cgaattgaat attccgattc
    28801 gcttcctggg cgttggtgaa aaattaaccg acttagtaga gtttgatccg aatcagtata
    28861 tccgcgatct attaaacatt gaatgaatag attaaaattg atcacagtat catccttaaa
    28921 tgcctataaa aactcataaa attctcttgg atacttttct ttagcagaaa attagctcat
    28981 taatgtctct aataccagct gttcggtctt ttgccattat ctctctcggt tgcccgaaga
    29041 ataccgtaga ttccgaaatt ctaaaaggcg gtctaatgca ggctggaatg gaattcaaag
    29101 ccgatccggc ggaagcggat gccgttatta tcaatacttg cggtttcatt caaccggcta
    29161 aagaagaatc tattgaaacg attctggaaa ccctgcgact caaaaaaaca ggcgtcaaaa
    29221 aagtggtcgt gatgggttgc ctctcccagc gttactatgc gcaaatcaag gctgaaattc
    29281 ccgagattga cggcttattc ggcgtcgaat cgcaaagtga ggtgattcga tttctgaccg
    29341 gtagccaaaa tatttgtccc gatctggaaa ctattcgcca gctaatgaca ccgcgtcatt
    29401 tcgcctacct gaaaatcgcc gaaggctgtg acaattcctg ttcattctgc gctatcccat
    29461 tgatccgcgg acgccaacgc agtcggtcaa tcgaatcact cctgcgcgaa gctgaatacc
    29521 ttaaatcaca aggagttaag gaattgattc taattgctca ggacactact cgttacggca
    29581 gtgatttgcc cggaaaagtt agtttaaaaa ctctgctgga ggagttactt gcggcacggc
    29641 tttttccctg gctgagacta atgtatgcca atccggattt ctggcgcgat gacttgaacc
    29701 atattcttgc tcaacatcca gagttttgcc catatattga tattccaatc caacatgcca
    29761 gtccccgcct cctgaagttg atgaaccgcg gtgccaatcc taaaaggctt aaggcgactt
    29821 tgcggaatat ccgccggtac cgccctgatg tggctctgcg cacctcggtt atggtcggtt
    29881 ttccttcaga aacggaagcc gaatttgaag aattgctgaa ttttatcgaa gaaatacgtt
    29941 tcgagcggct cggggttttt acttacagtg ccgaagagga cacagtcgct gaaaagtttg
    30001 acgacgatat tccggaaccg gaaaaagagc gccgccgcga tctattgatg caaattcaat
    30061 gggaaattgg tgaagacttt gcacgccgta aaatcggtca gaaaattacc gtgctgattg
    30121 aggagcaaga tggtgatgag tatatcgcgc gctctgtctg ggatgctcca gaaattgact
    30181 gcaatgttcg agtaaaatca ccaagaattc tctcaattgg cgatttttat gaagtaacca
    30241 ttatcggagt ggaaaactta gacctacgag ctgaaaccaa cggttaaaac ttttgttagt
    30301 taaatccaaa ttagctaatt tttggtcaga ttttagttta ataataatca ggatgaatga
    30361 acgatgaaag cattgataac agccggcgga cacggcacac gccttagacc aatcacccat
    30421 actcagaata aacacctgat cccgattgcc aataaaccaa ttctgaacta tgccctcgaa
    30481 tatgtccgcg atgccggaat tcaggaagtc ggaataatta ccaataaaga aggcaacgaa
    30541 gtccgtgaag tttacggcga cggccaagcc tttgggctga aaatcgttta cattcctcag
    30601 gaacgaccga ccggtttagc cgattgcgtc cggattgccg agccgttcat tggcaacgaa
    30661 ccgtttgttt tttatctcgg cgataatata atcatcggcg gtatccgacg ctttatcgag
    30721 gaattcgaac gtaaccagac caattgtcat ctcgtactag ctaaagttcc tgacccagaa
    30781 cgtttcggcg taccggaaat caaaggtaac cgtatcgtca gtattgtcga aaagccccaa
    30841 aagccactca gcgatttcgc cgtgaccgga atttatattt atgaccacac cattttcgag
    30901 gcagtcaaca atatcaagcc gagtgcacgc ggtgaactgg aaatctctga tgcgcatcaa
    30961 tatttattaa ataaaggtta caaagtcacc ttttcggaaa ttaccggctg gtggaaagat
    31021 accggcaaac ccagcgatct gttagaagcc aatcgtttag ttctggataa tatcacaact
    31081 tctatccaag gcaaagttga tcaaaatagt cagctcagcg gtcgcgtggt catcagtaaa
    31141 ggcgccgaaa ttatcaatag caacatccgc ggtccggtca ttatcggcga aggtgcaaaa
    31201 atcgtcaatg cctatatcgg tccatatacc gcaatagata aaaattgcga aatcagcaat
    31261 gctgagattg aattcagtat cgtttcctgt aactgcgtga tccgcaatca aaaaattcgg
    31321 atcgaagcca gcctactcgg taaaaacgtc cagatcatcg aatcacaagc taaaccgttt
    31381 accaatcgtt tcattatcgg cgatcaaagt ataattgaaa tcaggcagac ttgattgcaa
    31441 cggtcgcatt tcgaacgctt ggctgtcgcc tgaatcaggc ggaaacagat gccattaaag
    31501 ccgaacttgc caatcgtaat ctccacatag taaattccaa taaaccggcc gatttgacta
    31561 ttattaactc ttgcgccgtg acgcaagccg ccgaagccaa gacccgcggc gccatcactg
    31621 ccgcgcgacg catttcgccc aatggcaaaa ttgccgttat cggttgctac tcccagattg
    31681 ccgccgccga attgcttaat atttccggtg tctttctcgt gctgggtaat aatgagaagt
    31741 acgaaatatt cgattttctg gaaacgatcg agcgcggtga aaaaattgta cgagttacta
    31801 aaccggaatc tccaaaaact ttccgcatcg aatcgcactt accgaatgat aaacgcattc
    31861 gagctaattt aaaagtccag gaagggtgtg attattattg cgcttattgc ataattcccc
    31921 atttgcgtgg caatcctcgc agtcgcgatc cggaggattg cattgccaca gctcgacttc
    31981 ttgagaatca aggcgttcag gaaatcgtcc tgaccggtat caatcttggt cgctatgaat
    32041 caccgagcgg cgatctattt tttttaatcc ggcgccttct cgccgcgacc ggtttgccgc
    32101 gtatccggct gagttccatt gagccggact taatcacttc ggaattaatt aaactgattt
    32161 ccgaagagcc gcgcctttgt cgccatctgc atatcccttt gcaacacggt tccgatccaa
    32221 ttttagccaa aatgaatcgt cgttatctca gcgccgatta tgccacactt atcaaccgga
    32281 ttaaacaaca aataccagag atctgcatcg gcgccgatgt aatggtcggt tttcccggcg
    32341 aaactgattt ggaatttgac cagatgcgcc aatttctcga aaatctcgac attaactacc
    32401 tgcacgtttt ccgctattcg ccccgtcccg gaaccccggc tggcaaattt accgaccgta
    32461 ttcccgattc cgaaaagaaa cggcgctcgg aaattctgcg ccaattaagt aaccgaaaac
    32521 gggctgcttt taggcaatca atgctcgcaa aaactgtctc cgttctattt gaagaaaaaa
    32581 tagcacccgg actctggcag ggcttgacgg acaattacct aaaagttcgc atccacacac
    32641 gagaaaacct gctgaacaaa atcgaaaaag ttaaattaat ccacgtttcc gaagattata
    32701 tagaaggaga attgctcggt gagtgaacat ccctatttca tcggaattgc cggtaacatt
    32761 ggcgttggca aaaccactat gactgacctg atcgcaaggc gttttggttg gcgcgctttt
    32821 tacgaatccg ttattgacaa tccttattta gaagactttt atcaagatat gaagcgctgg
    32881 agctttaatc tccagattta ttttctctcc catcgtttcc gcagtcataa acaaatgacc
    32941 gaaagcaaca tcaccacggt tcaggatcgc acaatctacg aggatgtgga aatcttcgcc
    33001 cgtaatcttt acgaaatggg caatatgagt cagcgcgatt gggacaatta tcgggctctc
    33061 ttcgaaataa tgacttccta cctgaaaaaa ccggacttga tcgtctattt gcgcgccagc
    33121 gttgacaccc ttctgacgcg catcaaaaag cgttcgcgcg gcttcgagcg tgacatttcc
    33181 cccgaatata tctatacctt gaatctctct tatgaacgtt ggataaaaga ggagcagaag
    33241 cgtagtaata ttttgattgt cgaaaccgat cagcttgaca ttttccgcga tcaggagaaa
    33301 ctggaagaaa taatgcagaa aattaaacaa ttctgtccct gacgaagcaa attccaaagg
    33361 catagtagtc agaaatgata ttgcgctcga ttataatcca cacccacact tacgcggatt
    33421 atccgaaatt ttggattatc ttagccttat aaaaaatgcc gatagatttc actaaaataa
    33481 cgattttcaa accttgctct ttcctggtta aacaggacga cttaattaca cttatgcaag
    33541 agattaccgg gtacccagga gcaatcaggt tttctattga aaaaaataac ggtgaaactc
    33601 cttccttagc ccaaatcttc caaatgcgtc aaaataataa acaattaatc tcgccgaatg
    33661 aacaatatca tcaattagta ctttttcagc atttcttgcg ggaattgacc gccgcactga
    33721 aatataacat tttaaaaaat accagtgtcg cctttcgtat gcgcgtggaa cttgctaatc
    33781 ttttgaaaga aaatagggca ttgattcagc cggccgctat cgttcgattg gttgactgcc
    33841 tcccaaccga aaccggcttg gcgcttgctg gtggtacgta tgaattacac ggcaaaagcc
    33901 ttcaccgttt tgtgaataaa tccgacccag taaaacctaa accgatcttt atatttattg
    33961 taactattgg ttcggcgatt gataagcgga ttgccgattt aaattacacg gaaggcgatg
    34021 cctatcaggc atttctatta aacgggctag ccgccgccct ggtcgaaacc gctaccagtg
    34081 atttacaaca gtatcttgaa aaatcgtcct cctggttatc tcccagccag actttgcgcc
    34141 gcgtcagtcc cggtttccgg gactggcccc tcactgatca gaaggttatt ttcgaaatac
    34201 ttaagccatc ggaaaatatt ggggtgactt tatccgctga ctgcttgatg caaccgctga
    34261 aatccacttc tggaattatg ggcgtcattg aaaatgccct ttgagcaagt cggaataagg
    34321 caaaggctga gaaaagagaa aatgtaaatt gagttgttat tacaatctat cacggattaa
    34381 cacaggacta ctgacatgcc gaaacaatga tgaaaaacac cactaaatct acagaggaat
    34441 agccacgtag tagcttccta tttatactaa atgagatgga atttgggatg tcctccaaat
    34501 aaatccattt ccttgcaaat aatctatatt ctgcctaaaa tcaaagcaga atggcgccaa
    34561 aaaataatag tcaaccaatt tataatctgt ttccgcggat cggtaatccg ctcagtctta
    34621 gtcaagagat tgtcgaacat attgaagaac taattcgtca aaaaaaaatt ctgcccggtg
    34681 aaaaattacc gtcggaaaca gaactatgca aaatgtttgg cgtgagccgc acttccttac
    34741 gtgaagcgct ccaaaaattg agtgcccgcg gacttttgcg cattcgcaag gggagcggca
    34801 tatacgttac tgattatcaa gccgaaaatg taatcaaacc gatgagtctc ttcctagagc
    34861 tgaaccttga caaagattat ctgatgagtg tgatggaaat tcgcaaaatc ctcgaaccgc
    34921 aaatcgctag ccttgccgcc gcaagacgta ctgctcgcga cttgacgaaa ttgcggaaaa
    34981 attgtaatga tttgaaaaaa tgtgattcca ccgattatct caaacagggt cagatcgata
    35041 aagattttca tctcaacatt gccatcgctt ccaaaaacca attagtgccg attatcgtcg
    35101 aaccaatgtt ccggctaatg ccgaaaattc gcagtctggt gtatcaacaa attgacagcg
    35161 caaagagttc agccatcact taccatgacc tgattttaga aaaaatccag gttaaagact
    35221 ctcagggtgc ttttgaggct atgacaaccc acctgcaaat cgctgaggaa catactctcc
    35281 agatcattac cgatttggct aaataaatta tttttctaaa ggaaataagt tctttttagc
    35341 cagttaataa taaaaacact tgcgaaattt gtatgacaac tttaatattc agcagtttaa
    35401 aattgggaat gattctataa taaaaattaa ttcaaacctt taaatcataa taagggaata
    35461 gaatggaccg acagacaatt actgcaaaat tgattgaatc aggactggtt gccgtaattc
    35521 gattagatag cggtgaaaaa ttaccaggca taatttccgc tttgagacag ggcgggataa
    35581 atgcgctgga aatcactatg actacgccgg gagcactgga gataatcaaa aattttgcgg
    35641 cgcagaccgg caaagatttt ctcattggtg cgggaagtgt tctggatgcc gagaccgccc
    35701 ggctggcgat tttaaacggc gcccaattta tcgttggtcc gatttttaat ccggaggttg
    35761 ttcggatgtg tcatcgctac gacaaacctg ccattcccgg agcattcaca ccaaccgaaa
    35821 ttctgaccgc ctgggaacag ggtgccgatc tggtcaaggt ttttccgact acagcgcttg
    35881 ggccaagtta tatcaaagac attcttggtc ctttaccaca agtcaaacta ttgccaaccg
    35941 gcggagtcac cctcgaaaac gccgcagaat tcatccggtc tggagcttgc tgtctgggag
    36001 tcggaacggc gctgttgaat aaaaagatga tagccgccga tgattggcag gctttagcca
    36061 aacatgccga agcttttcga tcggctgttt tgacaggtcg ttccagtaag taaaaaataa
    36121 ttaatgaggt ataatatgga aattcgctat ttagccgatc caattcgatt tcaacgtatg
    36181 acaacgtctg aattacggca aaatatgttg gtcgaaaatt tatttcagac cggtgaactg
    36241 cgtttagttt acagcgatgc cgaccgcgcg atcgttggtg cggctgtacc ggcaaacaaa
    36301 ccactgacgt tgagcggttc aaaggaatta gcggcggaat attttaccga gcgccgcgaa
    36361 atcgggatta taaatattgg tgaggcgggt attgtatctg tcggagaaga gtcatttcat
    36421 ttagcccata aggaagcctt atacattggc aagggacgac atgacattgt ttttcaaagt
    36481 gattcaaccc aaaagccggc tgagttttat ctacttagtt atccggctca tcaagcctat
    36541 cccacccgta aggttaccca agcgcaggct aatcgtcgtg atttaggtag tgacgccgaa
    36601 tgtaacaagc gtaccatttt tcaatatttg cgtccgggag tgcttgatac ctgtcaaata
    36661 gttatgggac ttaccgaact ggcggaaggc agtgtctgga atactatgcc gcctcacact
    36721 cacgaacgcc gcaccgaaat ttatttatat tttgatctac caaaaaatgg cctcgttttt
    36781 catctttgcg gtgagccgga atctacccgt catatcgtga tgcataataa acaagtcgta
    36841 ttatcaccga gctggtcaat ccatgctggt gttgggaccg cccattatac ttttatctgg
    36901 ggaatgggcg gagaaaatca ggatttcgac gatatggacg atattgatcc agtcaatttg
    36961 cgatagatat aatgaccggc tgaagcccga tttaaagatt tattttttat cagataaatc
    37021 ttaattgatt ctacgacgat atttagataa atttgaatgt ttatttaaat gtggttgttt
    37081 tgaaaaatat tgctgttttt gcgtccgggc gcggttctaa ttttgccgct atccttgata
    37141 aaattcaaaa gggcgagata gccgcccgag tcgtctgcgt tataagtgat aacgaacgac
    37201 ctccggtttt tgagatagcg aatgcggcgg gaattccgaa tttctgcctt aatcgtaaac
    37261 aatttgcgac gccggaagag tatgccaatg ccttattgaa cgtgttagaa aaatacgacg
    37321 ttgatctgat cgttttagct ggatacttga agttgattcc gagcccggtt gtaaggaaat
    37381 atccacgggc aattgtcaac attcatcccg cacttttacc aaatttcggg ggaaaaggat
    37441 tttatggaat gaaggtacat gaggcagtaa ttgcttccgg tgtcaagttt agcggggtaa
    37501 cgattcactt tgttgatgag agatatgata cagggtccgt catttgccag gaaaaagtag
    37561 cggttttgcc tgacgacacg ccggaaagtt tagccgctcg cgttctggaa gtcgagcatc
    37621 gcctttatcc ggaagtggta aaagccctgt gcgaagataa aataaaattt gtaaatggaa
    37681 aggttgtttg ggaaaaatga gtgagttcaa aattaagcgc gcaattatca gtgtctatga
    37741 caagtataat attgggaatt tagctcaggc tttagctcgg aaaggcgttc aaatattttc
    37801 gaccggtgga acttatagag ctttgcaaac tgccggtatc gcagtggaaa aaattgaaga
    37861 tttaaccggt ttcccggaaa ttctcgacgg gcgggtcaag actctacatc cggctgtttt
    37921 tgccggattg ttggcggatg cgactgatga aagtcaccgc gctgatttgg cgcgcgtcaa
    37981 ggttgagcca tttcaattag tcgtggttaa tctctacccg ttcagcgaaa cttatcattc
    38041 cggcaataaa agttttgagg aaatcatcga gatgattgac atcggcggac ctagtatgtt
    38101 acgggcggca tccaaaaatt ttaagaatgt cgtcattctt tctgatcccg gacaatatga
    38161 tgaatttctt caacggctcg accaggatga attgactctg gaatatcggc aaaaattagc
    38221 cgcggctgtt tttttgaaaa cgatgaccta tgactcagaa atctccaatt ttttaacggg
    38281 cgccggagct gaaaaaatgc cggaagtcat tgttcaggca tttcataagc aacagacttt
    38341 acgttatggc gagaatcctg atcagagtgc ggctctttat gcaccactca ataatccgaa
    38401 ctggcagcct ttccagcaat tacagggtaa ggaaatatcg ttcaataatt atgttgattg
    38461 cctggcggca tacaaatgtt atgctacttt caaaactgag cgagccgctg ttgtcatagt
    38521 caaacatact aacccgtgtg gtttcgcaat cggagatact acgctggaag cttatcggcg
    38581 ggcagttcag accgatccgg taagttactt tggaggtatc gtcggggtga atcgggaaat
    38641 tgatgccaat ctggctgagg aatttactaa gagttttctg gaatgtatta ttgcacctag
    38701 ctattcgaat caagcattag aaattttaag caagcgtaaa aatttgcgat tactgatgcc
    38761 aaacgccgaa gcgttgcact atgatttgga tttacggtct tatggtcaag gccttctggt
    38821 tcaggaaatt caagccgccg aggatgatga gagtcaatgg aaaattgtga cagaaagcca
    38881 acccgatcca agccttatgc cgactgtgcg tatggcttgg cacgttgtaa agcacttgaa
    38941 atccaatgcc attgtcttct gcgatgcaaa tggtacggtg ggaattggcg ctggtcagac
    39001 gtcacgagtt gattcggtca aaattgctat tcgcaaggct ggcgaagccg gtttgtcaat
    39061 taaaaatacg gtaatggctt ccgacgctta ttttcctttc cgggattcgg tggatattgc
    39121 ggctgaacaa gaagtaattg gtttcattca accgggtggc agtattcggg atcaggaaag
    39181 tatcacggct tgcaacgaac gtaaattatt tatgatattt acaggtaagc gagtttttaa
    39241 acattaaagg gtagcaaatg aaaatgggat taaacttatt caacattttt actggcgata
    39301 tagctattga cttaggtacc gctaatacaa ttatctatat gaaaggaaaa ggtatcctct
    39361 tggatgagcc gtcaatcgtt gctcgttcga ctcacgatgg tgaagtattg gcggtgggcg
    39421 cggatgccaa ggatatgctc ggcaaaaccc accacgatat tatgactatt cggccaatga
    39481 aagacggggt aatagccgat tttgaaatga ccgatgcgat gattcgcggt ttcattcgca
    39541 agattaatct tagccggatt gctcgtccgc gaatggttat ttgtatacca agcggcatta
    39601 ctgaagtcga aaaacgagcg gtgcgtgatt cagccgatcg ggctaatgcg cgggaaatct
    39661 atttggtaga tgaacctgtg gcggccgcca tcgggatcgg gattgacata tccaaaccgg
    39721 tcggaaatat aattgtcgat attggcggcg gtaccaccga aatcgccgtt atcgctctga
    39781 atgggattgt caccaaacag gcaatccgcg tagccggtga tgaaatggat gaagccatcg
    39841 tgcagtattt taaaaccgag cataatctgt taatcggtga acgcaccgcc gaatatatca
    39901 aatgctccgt cggctccgct atgccgatca atgacacgac tatccaggtc aaaggccgca
    39961 gtctcgtagc cggtattccc aagactatcg aggtttcttc agtcgaaatc cgtgaatgtc
    40021 taaaagacac actcgatctg attattcaag ctattaaaca ggctttagaa aatacacctc
    40081 cggaactttc atctgacatt ctcgatcgtg gtattatttt gactggcggt ggcgcgctcc
    40141 taaagggact tgatcaacgt attcgggtcg agaccgactt gccggtcaac gtagccgaaa
    40201 cgcctctaca ctccgttgtc aaaggggctg gtatgattat tgaagatccg gaaaaatatc
    40261 gggatgtatt agtctaaatg ccatgtggct gaaaataatt cgttttataa ttgatcatta
    40321 cgaatattta ctgctaatat ttttaatttt tctctcatta tttatcttgg ctcataacaa
    40381 cagtcctcaa attagagcga ctcagggaga gctagccgac gcatttgctt ttttgcatta
    40441 tccaaaggtt tggatcaacg aattgtccaa tcttgtcgaa gaaaataagg aactaaagga
    40501 agaaaatctg cggctggcgt tgcttaatgc ccaattgaaa gaggcttatc tggaaaatca
    40561 acgcttgcgg gaaatgatga attttgccga tacgtctcga ctcacaattt tgccggcgga
    40621 agtgatcagt caggggacaa caccgcttta caattcgatt atgatcaatg tcggaagtaa
    40681 gcatggtgtt ggtccgaata tggcggtcat tactaacgat ggagtcgtag gtaaaacagt
    40741 tgcggtgggt gataataata ccctggtgca gatttttctg gatgtgaatt ttcggttggg
    40801 agtaaaattt cagatttcgc gggaacatgg gattatgcgc tggcgaccaa atggtttagc
    40861 cgaagtccgc gagataccga aaacggctat tatctatcct ggcgaaaaag tattgacttc
    40921 cgggttcagc gaaatttacc cgcccaatat aattgttggt gaagttgtag aggtccatca
    40981 atcagccaat aatctttttc agattgcgac tgtgcgacca agtgtgaata tcaattcgat
    41041 tgaagaagta tttgtggttt tgagtaaata atgttacgaa agattctcct gataattgct
    41101 ttcagtctgg tgactataat aatccagata ctgctcggac cctggatgac aatccgcgcg
    41161 attcgaccag attttctgct aatactggta ttgtttgttg gtcgtagtga aggaaaagtt
    41221 gctggtcaaa tctatggatt tttgatcggt ctaatcgctg atgcactcgg attgggctca
    41281 tttctgggtt tatctgcttt agccaagacc atcgccggtt ttggagctgg cttgcttaag
    41341 aaacgccgca gtcatttaaa tcccattttg ttttatgcca tcgaggtact gattattata
    41401 attcattttt caattatcta tctgataaac ttcaagggag cagagctttc gacccagact
    41461 atatttttgc gatatatttt accttctact ttatatacgg gagtttttta tttcataatc
    41521 caatatttcg ctccattttc tctggaataa agtatgtata tttcccacaa ctcaattact
    41581 aacatcagga aaaatatttt gacgattttc tttgtgctag tttttctggt tttgatcggt
    41641 cgtttatatt atttacaaat tatcaaaggc ccaagttata aaggtattgc cgaagctaat
    41701 cgggttcggg ttattccaat tgaagctccg cgaggtataa tttatgaccg taatggaaaa
    41761 ataatcgttg acaataaatc acaatatgat atcgatgtta tcccttatga attcagtcat
    41821 acggctcgaa cggattcaat tctggcaaaa atcctcgggc tcgatgtcgc agaaataaat
    41881 tctcgcataa aaaataactg gcggggtcag tttgtcccaa ttaaaatagc tgaggatgtt
    41941 aatgtcgaaa taattactaa tattgaagaa caccgccttg atttgccggg tgttgtttat
    42001 tcggtaaaac cggtgcgttc ctatccgtcc ggagttcaaa tgtctcagtc actcgggtat
    42061 gtccgggaaa tctctgccaa tgatttaata cgagaaaaat ataaggaata taaacaaggt
    42121 gacttgattg ggtggaaggg aatcgaacgt cagtatgaac atattttgaa aggcactcgt
    42181 gggtataatt tcattcaggt ggatgcttta ggacgggaaa tcggtcaggt agtagatcga
    42241 gataatattc ctccaattcc gggaaatgaa atgtttttgg ctatagatct tgacctgcaa
    42301 atctacattg agtcgttgac tgaaggatat aaagcaggta ttgtagtgtt ggacgccgat
    42361 aatggagaga tactggcgat tgtcagtaaa ccggactacg acccggcgtt attcgccggt
    42421 gtaatttcaa atagctcttg gaatgcattg ataagcgatc ctgacctacc aatgtttaat
    42481 cgagctactc aaggaacata ctcgcccggc tcgaccttta agctaatcgc agttttgacc
    42541 gctctggaaa ccaaagcggt tgatcccgat tggtcggtgc gatgttatgg tcagtatcgt
    42601 ctggggcacc gcatttttaa atgctggcaa gagcgaggac atggatcaat gaatatgtac
    42661 gaagccattg tccattcctg taatgtttat ttctataatt tgattagacg gctggatttg
    42721 aatgattggg caaatatggg aaaggatttt aagtttgggt ccgagaccgg ggttgatttg
    42781 tacggcgaat cagcgggagt gatgcccgat gtagattttc tgaatcgcaa atacggcgaa
    42841 catggctgga cagacggcaa taaattaaac ctcgtaatcg gacagggtga tattctggta
    42901 acgccagttc agatggctcg cttctgtgcc gctttagcgt cgcgtggtaa attggtgcaa
    42961 ccgcatgttg gattaaaata tatcgaccga ggaagcaatg agacaatcac ttttcaagcc
    43021 gccgaagact cgattcaaaa tatttctgtg gacacctggg aattcattga gaaggcaatg
    43081 gctgatgtca ttaaccgcgg caccggacga gccgcgcggg tcaaagatgt cccagtttgg
    43141 ggaaaaaccg gtaccgccca gaacccacac ggcgatgccc atgcctggtt tatcggatat
    43201 atgaaggaac aaaatcaaac catcgcaacc gcagttttgg tggaacatgg taaatcaggc
    43261 ggcggaatcg ccgctcagat tgccggacag atttttaatt ttattcacca acaaaaaaag
    43321 aggcagtcct tagcaaacaa tgcgccgctc aatttctaaa agaataaaca attttttaaa
    43381 ctccttttcc aacttgaacc tggcaatttt agtaattgtt ggattattgt gtttgttcgg
    43441 aatgctatca atttatagtg cctcattaaa tatggaaggt tccttttttg agaaaattat
    43501 cggacgacag ttgatctggt tagtattgag tggtctggtt gccgcaacta tttatttttt
    43561 acagaaaaag ttgatttacg actcagctta tattttgtat ttgttaggtt tttgcctcct
    43621 gttgttaccc tattttctgg gtagctcggt ggctgggaca gcccgctgga ttacttttgg
    43681 tccagccggt tttcagccat cagagtttat gaaaataatg gttatgctgg cgattgctcg
    43741 tttcttagcc agcacatcat cttccaactt ggattttaaa tttgtgttga ttcctactct
    43801 actctcttta ttaccaatgg ctgtcgtgct taaacaaccg gatttaggaa catcaattat
    43861 ttacttcggg atgttgtttc cgatgctgta ctgggcaggc gcccgtttat tccatctttt
    43921 tattatcatt gccccgattt taagcatcat aaccgccttt aatttttata ctttttttat
    43981 ctgggtaatt atcgtagtta tcattttgta cttgaccaaa gaaaaactca gggtttcttt
    44041 aacggttatg ttgataaatc tatcgcttgg ttttaccact ccgtttttat ggaatagttt
    44101 aaagccttat cagcaacaga ggattttgac actattcaat gtcaatgccg atccgcaagg
    44161 taccggctac caggttcttc aatccaagat tgcaatcggt tccggcggtc tttttggaaa
    44221 agggatagct cgaggaacac agacccacct taaattttta ccggaacagc atactgattt
    44281 catattttct gtaattggag aagaatatgg gttcatcggg gtgttagtgg tattactact
    44341 ctttttagct ttcattctga tttgcattaa tattgcttat cggcaaaaag atcgcttttg
    44401 ttcactttgt atgattggga ttgccagcat aatcggcata catgtggtca taaatgtagc
    44461 aatgaccgtt gggctgatgc cagttaccgg actaccactt ccatttatta gttatggtgg
    44521 atcttttttg atgacctgtt ttattctggt aggattggcg ctcaacattg gagtagataa
    44581 acaatattaa attttcaaaa gctgattgga aaataaattt ttctttttac cgagaaattt
    44641 cgtagctttc gtcacaattt atgtcattaa taatatgcaa caaccaatca aaaataacct
    44701 aaaatttaag ccatctgcca ttaaactttt tgttcttgca ttatccctaa tggtaataaa
    44761 tcttgatttg gctcgttcgg actccaaaag gctcagtaat gccgaaatag ccgcaagtct
    44821 cgaccgatta gcaatggata tttccgattt aaagacccaa atcgcaaatc taaccaaaac
    44881 ggtcaattca ctgaccgcca aagacaaaga gtctataact aaagctaccc taaccactga
    44941 catcaataaa ttgatcgaac cgcttcaact gactatggct aaccttgaaa acaggctcaa
    45001 tattctttcc gctgaacaag ccaatatgca gaaacgtttg aatactgtcg aacaaagaac
    45061 acgctatgcc gatagtatca attttgaaat tctatctcag ctagttattc tggaaaatcg
    45121 tttagcatcg ttgtctaata gtatcagtga ttacaattca gccagtcaga cagatgctcg
    45181 ccaagcaaca aatcgcgcta ttacttcgcc tggcatcacc tataaagatc ggtatttgaa
    45241 cgccttgagt ttgcaccaga aaggtaaatt tgaagaagcc atcgccctat ttcgcaaatt
    45301 aatcgccgat gatccgacca atgagctggc tgataatgcc caatattgga tcggagaaag
    45361 ttactattcg atgaaacaat atcagcaggc tatcattgaa tttgaaaaag tccggaattt
    45421 tactaatacc gataaagatg acgatgccca atacaaaatt ggactatgtt ttaaacaaat
    45481 gggcgaccag aaacgagcaa aagatgaatt ggaaaaattg attaatctgt atccgaccag
    45541 tgaatattca gaacgcgcca aacaattaat taaataaaaa cacttatgat taagagaata
    45601 tactttttaa cctctgagat aatcccattt gccgaaacct atcaactggc gattttttcc
    45661 aaggaaatcc caatggtttt caacgagcgg aaatacgact ttcgcttgat gatgcctaaa
    45721 tataacttca ttagtgaaag acgctatatt ttacgggaag tgattcgctt acgtgaaatg
    45781 gacctccagt accgtggtga aaatatcaaa gcctcggtta aatcggcttt tatccccaat
    45841 accaaagttc aggtgtattt tctggaacat ccttattatt ttaataattc cgataaaaat
    45901 ctctacccga ctgaatgtgt ctcggacgaa tatcttaact cggtacgatt cggctatttc
    45961 gccatggcgg cgcttaaaac cctgagctat ttgcgctgga aaccggaagt aattatctgc
    46021 aatgactggc aaatgagtct catccctctt tttatccagt ctaatttggt tgataaagag
    46081 tattgcgaag gtattaaaat cctgcaaatc atccattcca aagttgactg ttctaatttt
    46141 aaagtaacag aataccaaaa aataggatta aacaatcttg atttgtcaaa aagcgtagat
    46201 ggacgattag acgtagtagc cgcggctatg ccctattgtg atcatgtatt aatcattaat
    46261 aaaaatgaaa atttgctgga aaaatatcag aaagatccta ttttcagcga aattatgaag
    46321 caggataaga agaaattctc ttcttacgag ttaaatggtg aatcccctga agattggcaa
    46381 aaattggcag atgaaatcat caatattatg gaacagctct aaaccatatt tcaaccgtgt
    46441 ctggtcaaag ttacattatg taaaaattga ttatcgccgg agtgtggcgg cattcttttg
    46501 ccttttgttg ttcggttgtt ccgagaaaat accaactctg ccattagata aaaacttatg
    46561 cagtttcgat tcagttagcg taagtgctga atttattgtg gatacgaccg tttttgccga
    46621 acctaatatc ggctatagct cctatttagt ggtaggaact gacgctaatc ttacagctta
    46681 tagcctttta tcgtttaaaa ctttgccaac accgcttgat acccttcccc ttgctaaaat
    46741 cctctcgtgt gaattggttt taacgaccgg cagtgatctt tcgagtgaca ccgctgttca
    46801 cccgtcttta gaaatgaccg tcgcttctat cgaaggtgag gagaatgcgg attggtcaga
    46861 agatagtact aacagtacca atttcagcct tgatgattat catctaaccg attggaaaac
    46921 ttttacctac tcggatgctg atactttaat cctcgatttg accacatctt ttatcgccaa
    46981 ctggcgcgac tcaactcaat cccattatgg catcgtaatt aaaccggcgg tcggttcagc
    47041 tgccggtctg ggaataattt attccggaga aactaattcc tctccccata tcataatttc
    47101 gtatataact gataatggag atactatcag tacagctctt gcctgtgtcg gggatgtaac
    47161 tattacgcaa tttaaaaaga ccttcaccgc accgcctcag cccttgctaa tcagtgccgg
    47221 aaaagccgcc tatactttcc ttaaatttca ggtagaggaa ctcataacgg ataaaaattt
    47281 atttatcgct ggtgcttatc tttacttgca tgttaattcg gagctaaccg aagattacgg
    47341 aaattcgcac acaatttata ttactttgtt ggactcagcg gagtgggaaa gtggatcgtt
    47401 ttcaccatcg ggttcatcct atgttacttc acaaactttt tcggccagcg atagtatatt
    47461 gatcttgaaa attcccagta cggtacaact ttttaccagt ggcaatatta gtaatttcgg
    47521 ggtagcctta tggatttcgc caagtgggct ttacccggct atgctgggat tttatccgac
    47581 tgaagatccc gatccggcgc ggcggcctcg tttggaaata ttaacgatgc aagaagaatg
    47641 aaaacatctc gtcgtactat tttattccta ctgattctca gtctaaatgg ctggtcgcaa
    47701 atttcttatt atgccaacta cggtttcgga gttgaatcgc cagccaattc cgtgcgaata
    47761 ttaggaatgg gtcggatagg gacagccgtt agagactcga tgagtctgaa tcaatctaat
    47821 ccagcgctct ggcaaggatt cggcagtact tccttacaag gccagatgta cacctcggct
    47881 ctctcaattc ctgaccttga tttcaaaggt agaatagtaa atttttcggg cttttctttt
    47941 aaattgccgg tcggcaaacg tgccggtctc gcaattggtc tggcaccttt atctagaatg
    48001 aaatcttcgg ttagttttgc tgattctttg atatttcaag acgataaatt cgaatataat
    48061 tccgtagttg aattgtcagg gggaatttcg gagttatacc ttggtagcgg ctaccgggtt
    48121 tctccaaatc tggcaattgg cttgagagct aattttttgt tcggtaatta cgtggtccgt
    48181 aatcacacca agattgattc cgatattgac tcagaaagtt actataaacg cactatagcc
    48241 gttgacggta gccgcatcag cttcggcctt ttgttaagtg atcgcagtca acggtataat
    48301 ctggccgcga cggtcgaaaa agggaacgag ttaaaaaggt ggacaatcat cgaaaataac
    48361 tttggtcccg acaccactat aaaatccgat cctattaatt ttccgacaat tataaaggtt
    48421 ggaattaatt tgccgatcgc tagaaatcta gccttaaatg ccgatatgca atacggtata
    48481 tatgaggaag atctattcca aaatttctac atatttcaac agtttaaaag cgacagtcgt
    48541 aattcttatg ctgtcggaat cggcttagaa agatcaccac gtcaggaatt gaagagcacc
    48601 tttttccaaa aattatatta ccgctgtggg gcttattatc aaaccgaacc ggtctatcaa
    48661 aattctggga tttatgaata tggcgtcacg gctgggatca gtttaccatt tttttataat
    48721 ttgaatcgaa tagatttcgc attaacttac ggcttgaaga atggcttttt atcagacgaa
    48781 atcggcaatg aaaaaatact ggggattcac ctggggatta caaccggtga accctggttt
    48841 agaagatata aaaagcaata aaataaatga ggtgattata tgaaacgatg gattcatttt
    48901 attgaggaag cactaatcgt ttgccttgtc atcggattaa tgaactgcac tcctccttca
    48961 ccggaatcga gcgaatcggt tatggagcaa gttgaaacca agccgaccat tgatcgggat
    49021 aagtgcgacc tgtatttgag ttttgcttac agctattatc aaaatcaaaa ctggagaagc
    49081 gcaattgaaa attataagaa aatgagcagt tttggttgtg aagaagaata tgctaaggat
    49141 ctttttccct tttgggggag agcttaccag caattagcca gtgaaaatag cgtttatctc
    49201 gatagcgctc tatatgttta tttagcaggt gaaaaatatt tgccaaatga ccttttctta
    49261 aagaaaaata tcgcttatat ctaccacctg cagggtaaaa ttgacttaga aattcgggaa
    49321 tacgaaaaaa tgatcgaaat agcgccggat gatattgagc tctatcgcac tttagtcaaa
    49381 ctgtatatca gtgcccagcg ctacgaagac gtcatctatg ctactaacgg aattttaaag
    49441 atcaatccta atgatgagca agcccttaac gaccgaattt ttgcctacca gaagctcggt
    49501 agagacatta ctacaattca gaaagaacaa tgggaaaaga atcctaccaa tatccgctat
    49561 ggcttggagt atgccgccgc tcttaaacaa cagcgcgaat acgataaagc cctcgaagta
    49621 tatcaaaagg ttgccgaatt agacgctaaa aatattgaag cctggagcaa tattgctgat
    49681 atttatttca ctcagaaaaa taattatgct gaagccttga aagcctatga tcaaatctta
    49741 aggaatattt ctcctaatga tttaaatatt ctccaacaag ccgcccgatg ctgtcaatta
    49801 ttgggtgatt tcccaaaggg aatggaatat gcccgaaaag ccatcacaat tgcacccaat
    49861 tcgccccaag cttacaaaat tagagccgac ctctattttg ccgccgctga ttattatacg
    49921 agcggacgtc aaataacttt tgaggataaa ttagtctata aattagccta tgacgattat
    49981 ttaaaagtaa tggaattagg cgacaccagc gtcaaagcgc gggttgacca ccttaaagaa
    50041 tatctgattc caactcctga agaatggttc tttaaccgtt atgatgctaa cggggcaatg
    50101 cgtaccagct tccgacctct tagccccgaa tataaatgga tcacggtcga accgaagaag
    50161 aactaaatat ggtaattgca ttcggagcgg atcatggtgg ctatcgcctg aaaaacgcat
    50221 taatcgaatt cgcgcgttca gaaggctatc aagtgattga cttcggcgtc aacagcgatg
    50281 acagtgccga ctacccggat tatgcccgag cggttggtga ggcaatcgtc tctggccgcg
    50341 cccaatttgg tgtgcttatt tgtcggactg gcgccggtat gtgcatttcg gctaataaaa
    50401 ttcacggtat ccgagccgta atggcttacc aacctgaaat cgcccgactt agtcgcctgc
    50461 ataaccacgc caatgtgctc tgtttcgggg ctgattttac tacagttgaa tcagctaaag
    50521 aaatattgaa gacattttta gaaacgccat cttccgatga tttacgccac ctacgccgtg
    50581 ttgaaaaaat tatgaaattg gagagttgat aacttggaat acaatttcct ggctaaacag
    50641 gatgccgttc ttttagatac gattaaagcc gaaatcgagc gcgaacggaa aactctggaa
    50701 atgatcgcat cggagaactt tgtcagtcca gccgttctgg aagccgccgg ctcagttttg
    50761 accaataaat atgccgaagg ctatccggga aagcgctatt acggcggatg tgcggcagtg
    50821 gaccaagccg agaatttagc tcgtgaccgg gttaaagaac tcttcggggc tgattatgcc
    50881 aatgttcaac cgcattccgg ttcgcaggct aatatggctg tatatttttc gctggtaaat
    50941 gtcggtgata ccgttctggg aatggactta gctcatggcg gacatttaac gcacggcagt
    51001 cccgtaaatt tttccgggaa acttttcaga atcatttcct atggagtaaa aaaagacacc
    51061 ggtcgcattg attttgatcg tctggcggaa ttagctcttg agtatcgccc gaagatgatt
    51121 atcgccgggg gaagcgctta tcctcgattt ctggattttg ccaaatttag ggaaatcgct
    51181 aatagtgtgg gcgcttttct ggtagcagat gtcgctcatc cagccggtct gatcgccgcg
    51241 ggattacatc ccgatccctt gccatattgc cacgttgtta cttcgacgac tcacaaaact
    51301 ctacgtggac cgcgcggcgg cttgatctta atgggcaaag actgggaaaa tccattcggt
    51361 ctggtagctc ccaaaagcgg acgcaccaaa atgatgtcgg aaatcattga ttccaacgtt
    51421 atgcctggca ttcagggcgg accgctgatg cacattattg cggctaaagc cgtggcgttc
    51481 ggcgaagctc tccgtccgga attcaaagct tatgcccaac aaattatcgt caacgctcaa
    51541 gtcctggcgg aagaacttct tcatcgcgat tacaatctgg tttcgggtgg aactgataat
    51601 cacttgatat tgattgacct gagcaataaa aatattaccg gtaaacaggc tgagaatatt
    51661 ctggaagaat ccggaatcac caccaataaa aatatggtgc ccttcgatca gaagagcccg
    51721 tctgtcacca gcggcattcg cattggtaca gccgcgttaa ccacgcgtgg aatgcgagaa
    51781 gcggagatgc gtacgatcgc aggattaatt gaccacgttt taagcaatcc tgattccaaa
    51841 gatattcacc agaaagttcg ttcagaggtt ggcgaactat gtggcaactt cccactctat
    51901 caagagataa aattataggt gaattatgcg ttgtcctttt tgtagttctc cggaaagtaa
    51961 agtgattgat tctcgcaccg tgcaaaaaaa caatgccatt aggcggcggc gtgaatgtat
    52021 tgactgtggg catcgctata cgacttatga atatgtcgta gaatatccgc ttcaaatcat
    52081 taaaaatgat ggtcgccgtg aagaatatga tcgcaataag cttttaaata gtattaaaat
    52141 agcctgtaac aagcggccga ttcctgctga gaagctagaa actctggtaa ttgacattga
    52201 aaaggaaatc gaagaaactt ccggtagcga agtgccttca agtcaaatcg gtgaattagt
    52261 gatgaaaaag cttaaggaat tggatgaggt agcatacatt cgatttgcat cggtctataa
    52321 aaaatttaag gatttggatg aatttaaaga tcatattaat aaatttggag gaaactgatt
    52381 gtatataaaa aaggaacaat tgacagtatt ggtgagtttg ataaaactgt cgtcgttgaa
    52441 aacaatgaaa aattttcgag aaatatatgt tatctaatga attgcttgaa attttagcct
    52501 gcccgcaatg caagggcgaa cttgagtacg atatttcaaa ccagcaatta atttgcaacg
    52561 tttgtcgttt gaaatatgcc attaaagacg atataccgat tatgttaatc gaagaagccg
    52621 aaaaatttta aaaatttatc aggagtaaat tcgcaccttt ttgctaatcc catatttcga
    52681 agtcaataga taaatatcct tcgttaaaat tactcgtttg tgtatattat tcagaccgaa
    52741 agtattgatg ttcggttttt tgttcttagg aggttttgat gaaaagaata atcgccatgc
    52801 tcgggattat atcattaatg acggcgggcg ccttggctca aggcgaagcc ggtgccgttt
    52861 ggttattgat taatccaggg gcacgctttt ctggaatggg tgaagtggga gcggctcttg
    52921 ccgacgatgc ctatgccagc tactggaatc cggcgggatt gggattcctg cgcggtcggg
    52981 aattggcatt gacccatgtc aactggttac cgaacttagt tagtgatatt tattatgaat
    53041 ttatcggata tcgtcaattt atcagaaatg ttggcacagt gggcggaaac attatactgt
    53101 tgaatctcgg tgaacagcaa cgtacggatg aattcggaca tgatctcggc accttcaact
    53161 cctttatgct ggctgtaaca ggttcgtatg ggaccgctct tacgcgcacg tcttcgatcg
    53221 gagtgggtgt gaaaatggtt caccagcacc tgaccgacca aggcgccgga aaagaattag
    53281 gaaaaggcta ctcaacaaat ttctgcttcg atatcgctta tttaaaacat agttttctaa
    53341 ccaaaaatct tactctggga atcaatatgg ctaacatcgg gcctaaaatt gccttcattg
    53401 atgttaatca ggccgacccg atgcctacta atatgaaact tggtctggca tataagttag
    53461 gtaatgaata caacaaagtc acaattgcat ttgatattaa caaaatattg gtagccgctt
    53521 atccggatcg ggatattgac ggcgatgaaa aagttggtgg ctatgatgaa aatggtcatg
    53581 ctgtgcctgg tggtgaatat aataaaaagg gtcagcgtga atatgcccat tcagatgagt
    53641 ggtatacagg gatttttact tcctggtttg acgattggtt attaatgtat gattccgcca
    53701 cggatggcat cggttctgcg gataaggatg gtaataccaa tgacggctcg cttgcggcag
    53761 aattcgaaaa attgacgtat aacttcggaa tagaatattg gtactccaat ttgtttgctc
    53821 tgcgggctgg ttgtatgtat gataagcctg gcaatatcac gatgaaaggt aaatggccag
    53881 taccgacttt cggggctggt ttgcgctggg cgggtatggg attcgatttc ggctataccg
    53941 ccggtgatcc cggacatcct cgtcaaaata caatgttatt ctcgatgaat ataaaatttt
    54001 aattttagat aatcagaaca gttcttaccc tatttaaaaa aggctaaaaa cctaacacga
    54061 tgaaaatata taaatatttg attaccggga taacactaat tgttttagct aataaagccg
    54121 catacggtag gtttgaaccc cagtatcgga ttgcgactgc tccatcggtt gtgaaaaata
    54181 ctgcgcctgc agctattcgc atttgcgccc tgcgcgtcag ctttccggct gatgaagacg
    54241 aaaccaccac cggcactggg caattcctaa tgtccgccgt agagactccc tgtgatgacg
    54301 aattcgtagt tgatccggcg cctcacaatc gggcttattt tcgggatcat attcgctctc
    54361 tggcaaatta ttataatcgg gtttcccggg gcaaagttgt gatagacacg actaattcgg
    54421 aagtttttcc gctgaatgat aataacagtt atcaggttga ccatccgatg gcttattacc
    54481 acccgttttt ggtcgaagat tcggtggatt tgcgcttgac tgaactttta gttgagtcag
    54541 tccagaaagc cgatccggag gtggattttg cgcaatacga tgtcgtaata gtttttcatg
    54601 ccggagttgg tcaggatttt gccattacgc ttgatccgac gccttatgac attctatcag
    54661 cttttatgaa caaggatgat ctaaaggcat ttttagcacc gaacgattcc gactttcagg
    54721 gtattccggt agaaaacgga acggctttcg ttgattgcgg cttaatcttg ccggaaagcc
    54781 aaaatcactt gctatatgat aattgggaag aggtattcgg tgaaagcgat tccccctgtc
    54841 attaccaatt cggtttgaat ggcaccctgg catttatgtt tggtttttat cttggcttgc
    54901 cctgtttatt tgacacggaa agcgggatga ccggcatcgg cagatttggt ttaatggatc
    54961 agggttcggc taatttgagt gggctagtcc cagcggtgcc atgtgcctgg gaaagagttt
    55021 ttcttggctg ggaaacgcca gtagttgccc aaaaatggga agatgtcgtt cttaaaaaag
    55081 ttggctctgg ttccgatacg accgtttgga agatcccaat caatgctaac gaatattttt
    55141 tagtagaaaa tcggtgcgct cattttcgcc cgggcgtcag ttttgatagt cttcagtatc
    55201 gtatttatat ggaaaacggc atggttgaat ggccgctgag ttttcccttg attcgtgact
    55261 cactaaaagc cgaattttca ccggaaaccg gcgtgatgtt agcagtgccc cattatgatt
    55321 acggtttaga aggctccggt ttattaatct ggcacattga tgaatcggtc attaacgcca
    55381 atttagcgac taatagcgtt aacggtgacc gagaacatcg gggcgttgat ctagaagaag
    55441 gcgatggcgc tcaggatttg gggtacgaag ccgatatttt gaataaagat atcgaggatg
    55501 gatggttttt cgatccctgg tttgccggaa atgatggctt tattgatctt aattcggatt
    55561 atattttaga tgtggaggaa catgtcggct ttacgcctta tactaatccg gcgacgtata
    55621 gtaataatta tacttttacc ggcattagta ttgatagcat cggtccagct ggcgaagtaa
    55681 taaccttccg aattagactg aatgatgcgc tcccaaattt tccgcttagc ctgagcgcgg
    55741 gagccgacga aatattaccg atagatctga atccaaatga ccaggttgaa gaatttctga
    55801 ttttagccga cagcataagg atttatactt ccttcggcga acaaatagcc tccctgccgc
    55861 gccaaacagc gatcgcactg gataaagata gcttattact ttatggaatt gttcagaata
    55921 atcccgaggc gtcttctttg agtttatcta tctggaaatt tgggcaaaat ggccagatta
    55981 ataaaactga taccgtcagt ctgaattctc aggatttggt cagccctctt ctaccagtaa
    56041 agggaggtgt gctattcgct acccaagatt atgccgacaa ccaaaattac cttcacctct
    56101 atcagattga tactcaaaat attgagtcat tggagctcgc ttcacaggtt aaaatgctgg
    56161 tcggtgacga cgataatatc ttcggaataa cggatgataa tcaggtaatc aggattcatc
    56221 ttaattcact ttctgccgaa gtcgtagccg atttgccgaa cgccttatct ggaacaatgg
    56281 taatagcttt tatcaatcaa aatgaaagtg tggatttgat tcattgcgcc gattcattgc
    56341 taactcttgt tctcgacatc ggcaccccaa atgaggttgt tttacaccgg gaacaggaat
    56401 ttaacggcaa tttagcctgt gccgatttag atgacgacgg aaaaatcgaa attatcactg
    56461 ccacttccag ccaggttttg gcattcaacg aggaattgac agttaaaaac aatttcccga
    56521 tcggggttcc gaatatttta ggttccgacc ggttcgaatc ggaactcttg acagccgatt
    56581 tagataatga cggaaaaaag gatgtcatta tcaatatgtc ttcagctttg ttggcgttca
    56641 attataaagg agatttattg agcggtttcc ccaaaattgc ctaccagcag agccaaaagt
    56701 ctggcatatt attaaataca ccactcggca taacttattt agcactgacg tcaaccgaat
    56761 caaatcctca aaattacaat tgtataaagg ctgtgcggct ctcctctttt tcacatgatt
    56821 ctgaagattg gatttgttac ggtgggaatg ccgctcgcca attctattat ccggtgcaat
    56881 cccagagcag tccaattcag tccaagtcat tgctcgataa tgccaggact ttttgctggc
    56941 ccaatccgcc taaagataat cgtacataca ttcgttactt ccctaatagc agttgtaata
    57001 tttcgattaa tatttacgat ttggctggcg atctgattgc cgatttcaag gattccgctc
    57061 ctttaatcgg cgaaccaaat gaaaaggaat gggacgtcag caatgtcgaa agtggagttt
    57121 atttcgccgt agttcaagcc aaatccagtt cgcggaccga ttctaaggta attaaaattc
    57181 tggtggtgaa ataatgtctg agcgccagat ttgccggaat acgctggtca tttttctaat
    57241 aatgctcttt ttttttaatc cagccggggc acagttcggg acttataatc atcctgaact
    57301 ggaatggtta tccttcgaga ccgctcattt caaaatccat tatcatcaag gggcggagtg
    57361 gactgcaaga cgaacggctg aagttgctga aactatttat ggcccgataa ctgaatttta
    57421 tcaatttgaa ccaagtgaaa aaaccgctct ggtaatccga gataccgatg atatttcgaa
    57481 tggcgcctca tattattacg ataataaaat cgaaatctgg gctacaccgc tcgattttcc
    57541 ttttcggggc aatcacgact ggttgcgcga cgtcatcacc cacgaattta cccacattgt
    57601 ttccttgcaa aaggctatga aattcgggcg ctcaattccg gcaatttata ttcaagttct
    57661 ggatgatgaa ccggagaaac gcgaagatgt ggtttatggc tatcctcgga tcattgcctc
    57721 ttatccgatt tccggtatgg taatcccgat gtggctggcg gaaggtatgg cgcaatatat
    57781 gttccctgga aattctaatg atttctggga cacccaccgt gatatgcttc tgcgcgatcg
    57841 agttctgcat aaatcattgc tgtctttgtc ggaaatgggt tcatttggca aacgtggcac
    57901 cggcaacgaa tcggtctata atcaaggctt tgccttcacc cgttatctgg cagaacatta
    57961 cggaactgag actattccgc gcctggcaga ggaactttcc aagccagtgc ggatgtcagg
    58021 tgagacggct ctgaaaaacg tcactggtgt cgcggctgat gagatttacc aacagtggca
    58081 aaccgaattg gaacagagat atttcaaaca aacagaaatt atccgggcta acgaaaaagt
    58141 cggcgaaatt attctggctg agggtacgat gcagttagga ccggaatgga gcggtgattc
    58201 actgctttat tttatgtcca ataaaggtag agattattat tcccagactg ccttatatgt
    58261 atatgatttc catcaaaaaa aaagccggat gctagttcct cgaattaact cgagagtaac
    58321 ggtcgcaccc gacggacaaa acctatatta ttcccgggcg agtaaaccga atcggcacgg
    58381 ctcggtattt tttgatattt atcgctatga tttaaagaaa cgtaaggaaa aaccgctaac
    58441 caagagcgct cgggtctata atccaaccct ttcgccagat ggggaaataa tagcttttgt
    58501 gactggtcga gatggcacca gcaatgtagc cctaatgaat ctggactcta ataaaatcag
    58561 agaaattact actttcagtg aaggagtaga ggtttttacc ttgcactggt caccggacgg
    58621 gcgcacgatt gctttcgact atctgattgg tcatggccgt aatctggctt tggttgatgt
    58681 agaaaccgga gcgatgcaga ttttggatga ggcggcttat gatacccgca atccctattt
    58741 cagcccggat ggcaagtggt tgtactttgc ctcagatgag accgggattt acaatattta
    58801 ccggcaatcg ctaactaccg gcgaacggca attactgaca aacgttaccg gcggtgcttt
    58861 tatgccagcg gtcaatgaga aaggcgaatt ggtttatgct attctggacg atcgggcatt
    58921 taaaattgct tgtattaaaa accccgaacc gattgaaccg cagttggcag tttatttaga
    58981 ctatgatcaa aatattccac cggtttcgga gattcaaccg gtgtccattc cagaaccgca
    59041 gaaatatcac gatcaatttg ccaaaatgtt cattatgccg cgcttgatga ttgattacgg
    59101 aacggtcaag ccgggattct actgcctgtc aaatgagata atcaaccgct tcaatatttt
    59161 tgcgggggct tcgattaata gaatcaaaga ccgcgatctt tttctgattc tggaatatca
    59221 ccaatgggag ccgactatct tcttggaatt ttacaatatc agtcgcaata tctttaacga
    59281 gaagtcaatc tataaaggct ggccagtcga agcggactat actttttatt tgaccgaagc
    59341 ttatggcggc atttcccgcc cttttggcgg tttcaataat gttcgtttcg atgttaaatg
    59401 gtcgcgttac cgcaccagta gtgacgaaac catcacagcc gccgatattt atcaaaatgg
    59461 cttttcctat gattactatc gcggcgttaa ttttcaactg cattggcaga tgcggatgat
    59521 gttaccgaaa gtgaatgagg aaactaatcc ggataacggc attctgctgg acacttatat
    59581 tgcacgcaat tatgacaaat ttataaaagg ttttaaagtc aatagcagtt atagcactat
    59641 tcaagtagat tttcgcaata attattactg gcgattggaa catgaaggca attggtatca
    59701 caaatatccc ttatttaaaa atttggttgg cactttgaga tggcatttgg gctggatttc
    59761 taagccggat gttgattcat ttttctattt ctttgctggt ggcgaaccag gattgagagg
    59821 ttatccattt tacagtatcg aagggcgcaa tctaagcagt tggcattata cttggcgtct
    59881 gccacttttt cgtgagaaac attttcaaat cctgacattc aatttgcaaa atgccttcgt
    59941 agcgacttat attgaggctg gtaatgcctg gaatcaggta accggttttc cgggattgaa
    60001 atgggaacaa tttaagaccc aacctgtttc agtgctacgt aatattgtga atgattttaa
    60061 aagagatatt ggtttccaag tgcgtatgag tggtttttcg ttttattcct atcccacctc
    60121 aatcagcctg gactttgttt atgggctgga tgattttgag gtggtttcca attctaaaac
    60181 ttaccaatac ggacatgaat ggcgaactta ttttacgcta ttgtttggct tataagggag
    60241 gcaacatgaa gtcgaaattt ttagccgcaa cgctactttg cgcaattcta atccccatca
    60301 tgggattcgg ccagtcgttt ttcgatgaac gcacgacggt gaccgagcgt gaccgtttaa
    60361 tctatagtat gaaatatgcc caggctgatg aggatacttt cgtttttaat tatgagaaat
    60421 cacccggact ggctttcttt ttatcggcgg tcgtaccggg cgcgggcgaa ttttatgccg
    60481 gagccaagta ccgggcggcg gcttttttca gcgtagaagt cgtttcctgg gtcggctatt
    60541 tcaaccgcaa gaataatggg gaaaaattag aacgtaaata catgaactgg gcaaatactt
    60601 actgggatgc ctggcaatgg tatgaaaata gtctctccta ccccgaatac tttggacttt
    60661 ccgattccgg tagcggtttt ggttcccata ctatctattt cgaatatgag ggtaaagaat
    60721 acaaagccga tgtttcttat ctggatcgtg aactccctgg ttggcaaaca tatttacaag
    60781 aagggaccct cagaccaatc aaaacccgcg attattacga aaatatcggt aagtacgacc
    60841 agtttgcttc cggttggagt gattttgacc aaatcgtatc tgaggttgat tctctcaata
    60901 ttcccaccag ccctcttcgt aaaaagtatc tcaaccaacg ctatgaatcc aaccaatcgc
    60961 tcaaaatggc aaccaatttt acgaccgtga ttatgttcaa ccacctgatc agcgcctttc
    61021 atgctcaaat cgccgctaaa aattaccgac cgccagaaca gaaagatgtc agctggcatg
    61081 tcggcttggt gactgattgt acctataaaa tgccaattcg tggtttgtct ctttcagtgg
    61141 ctttctaaaa taatttaccg atcaaaccta cccgaaactg tgccggattg aaatgcctcc
    61201 caaacgtcaa atcctaaaaa ttgtcctgat tagcttgctg attatttatt taaacaactg
    61261 tgcctctaac aaacccaaac ctgttgtaga tttcgagcaa cattatcgcc aaggaacgga
    61321 atattttaat aagaaaaaat gggacaaggc gatcgagcat tttactttgg tagttatcaa
    61381 ttcccccggt agcgaactgg cggacgacgc tcagttttat attgccgaat gttatttcaa
    61441 gcgtaaagaa tatctcctgg cggcttcgga atatcagcaa ctgattaacc gctatgctta
    61501 cagcgaatac gtcgaggaag ccttttataa aatcgctctt tcacaataca aatcttcccc
    61561 gcggtaccag cttgatcaag aatattctat ttatgccttg caaagttttc aggactttat
    61621 tgatacctat ccccaaagtc gtttccgcgc cgattcagag caaaaaattg ccgagattcg
    61681 ctcaaaatta gcccgcaagc tttttgaaag tggaaaactt tatcgcaaaa tgcaggaatg
    61741 gaaagcggcg attatttact tcgataaagt gctggaaacc tactatgata ccgaatgggc
    61801 tgaaaaagcg cgactggaga aagcctattg cctaattcgt caacgccaat tcgatcagta
    61861 cgaacttctt tttaaggaaa ttcttgcccg ctctaccccc caaaccgacc cgaatgctct
    61921 tgaatatctg cgcacttctt atcacaaaga acttcgtaaa atagagagag aaaaacgcaa
    61981 gagagcagat tgaaagttgg cttattcggc ggaacgttcg acccgccgca taacggtcat
    62041 ttagaaattg cccgagccgc tatccgagct ttctctctaa acaaattgat ttttatcccg
    62101 gctaacatct cgcctactaa aacctcagaa attcacacct cgccggtgca tcggcttatg
    62161 atgctgaagt tggctttaca aaacgaacca gcttgcgaga tttccacaat tgaaatcgaa
    62221 cgcggcggga tatcttattc gattgatacg atccgggcta ttaaacacca aatgcgtctc
    62281 gagcgtgaag atttattttt cgtggtgggc gccgatagtt tgatagaatt tcataaatgg
    62341 cggcaaccgg aagatatttt acaagaagtt caggtcgtcg ttgcccgccg ccccggttgc
    62401 gatctaagtc tagtcaaacc ggaatatctg gctaaagtcc tatttttcaa tgcgccccta
    62461 ataccatttt cctcttccga tattcgccaa atagcgatga atggcggaca gatagatcaa
    62521 tttgttccgc ccgccgtggc tgagtatatt cgccaaaatg aactataccg ccggtcggct
    62581 taaattagtc tgagaatcca attatttggt aatcactcgc catccggata gaccgaggtg
    62641 atcggtataa actcttgtcc gaataataaa gcaaaatcga aagtccgcct aaagcctaac
    62701 tacattaaga aggcactttc agattgagag ccgattccag tggtttatgg cgcgtcgtaa
    62761 attgctccga aaacctcgat tcaggcgact aaaacttgta atcattgcat ttatatcttg
    62821 acgtttattg aaacttgcca tagggcaaac gggtgcgatg gatttgattc cataagctcg
    62881 tccgcttgct ctaatatgtt tcttgggcca attaagcaga ggccgaacga tgcgtatccg
    62941 cccgcccagt tggatctgaa tcggttggag cgattcgaga ttccctttga ataccaggtt
    63001 cattaaaccg gtttcgagat aatcgtcgag attatgccca aaagctatgg cgttacaatc
    63061 gtgcattatc gcaaactgga atagtcgttt tcgtctttcg cgagcgcaag cgtagcaatt
    63121 gatttgagac gattctggca attcactatc taatatcgaa aatggtattt gccagtcggc
    63181 taggagatcg gcgatttctt taagtgcttc ggtggtcaac gccaccgact tctggcgaac
    63241 ataagccgcc aatagttcca gatttttaaa ctcagccagg cgtaattcga ataaaaggcg
    63301 gcttagaacc agactatcaa tccctccgga tacggcaatg aggatttttt gattgtcggc
    63361 gattaaatta tactgactat tgagtttatc gaattgagaa accagcgaag ccatattagc
    63421 cggcggcgag tttaaaggta atgacatcgc cgtcgtgaac caggtaggac ttgccttcaa
    63481 tatgcactaa tccctgttcg cggagaagat tttcactgcg cagactttcg atatctttcc
    63541 aatggcgcac ttcagccttg ataaaccggt cttcaaagct ggtatgaatt tcggcggcgg
    63601 ctttgacggc tggagtattg accggaattg tccaggcttg gacgattttt gactccatag
    63661 tgaaaaaagt aatgaggcgg agcaattcat agccggcacg aattaagcga tccaagcccg
    63721 gttcggtaat tttccattca ttcatcagaa gtttccgatc atcaggcggc agttcagaca
    63781 attccagttc cagattggca gagacggtca ggaagagatt accggtttgc aaagcaaatt
    63841 tctcaaatgc ttctgtaata gccgatttat tccccgatat agcttcatct tcggaaatat
    63901 ttcccaggat gatgactggc ttgctggaca aaagcgccaa atcacgtatg aaatattctt
    63961 cttccgggtg agaatgatat tgcttggcag actgcccttt gataagcaga ccgcggatat
    64021 catccagaat ttttaattgt ttttgggttt gctcatcgcc gcttttggct cgggcaatta
    64081 atttttcccg gcggttttcc acaatttcga ggtcttttaa taaaatttcg gtttcgacga
    64141 tttcgatgtc gcgttgcgga tcaagttttt catcaatgtg ggctacattg ggattttcaa
    64201 agcatcgcac aacgtggata atggcgtcca ctgcttgaat atgactcaga aattggttac
    64261 ccaatccttc gccttgggaa gcgccacgca ccaacccggc gatgtccacg aattgcaacg
    64321 tagtcggaat gatcttgtcc ggcttataaa tctgggcaag caaatcgagt cgcttatccg
    64381 gaacatttac aactccgata tgcggattga tcgtacagaa tgggtaattc tccatggcga
    64441 cgccggcatg cgtcagggca ttgaataatg ttgatttacc aacgttcggc aaaccgataa
    64501 ttccgcattt aaaacccatt tattgctcct gatgaccgtc taatttcatc actaacgaat
    64561 atagaaaaaa atctataaca aacataattt taaaaa
//
LOCUS       DAOMJU010000002        50240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_524_length_50240_cov_18.088971, whole genome shotgun sequence.
ACCESSION   DAOMJU010000002 DAOMJU010000000
VERSION     DAOMJU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 50240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50240
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_524_length_50240_cov_18.088971"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PLO58_00305"
     CDS             complement(<1..1170)
                     /locus_tag="PLO58_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73264.1"
                     /translation="MKKGGYVLIMIAVLSTMAEVGWGQVSITQFETAYNQDFNSLANT
                     KTDNAWTDNSTISGWYSNRTEYIADNGSSTTGGLHSYGSADASERALGGLSSSSASPA
                     FGIRLVNNTGNTITSFNISYKGEQWRQTANSHTLVFSYQVDATGIESGTWTSFSDLDF
                     TALKTGTSSALDGNAEGNYQNVSSNVIIEVPNGHEIWFRWLKTGTNSPGLAIDDLSIT
                     AYEIKVPTISANPSSLSGFSYVVGSGPSAEQTFMVSGTNLTADISISAPEHFEISKTS
                     GSDFTSSLTFAQSDGSVNETIIYVRLKAGLAIGSYSSENITCSSEGADDRTVTCSGTV
                     TSPPMHYRSKITGNWGDNSTWESSTDQTTWSNASFSPTSNDYTITIQNGHTVTIDA"
     gene            1573..2493
                     /locus_tag="PLO58_00310"
     CDS             1573..2493
                     /locus_tag="PLO58_00310"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQO73265.1"
                     /translation="MKKAPKLLFVVNTRKRVHSISLSWGKLVAVLAGGVFLLIFVIIL
                     GANLFSNLVYQSRLTRLKKNNNRLVSTFYDLNNRIQKMESDISMLVEKDKALRTYGDL
                     PVIDQDIRRLGVGGRLMPKSDELDQLLPESDIKVSELVNNLDRLAREIKFERISYETI
                     YDAFKNRSDQIQSTPSIRPVYTGFINDGFGYRRDPFTGRREFHYGLDITTPTGTPVFV
                     TADGVVGKSGYQGGYGYVVEVDHGYGYSTLYAHLSKILVRSGEAVKRGQKIGEVGTTG
                     RTTGPHLHYEVKQFNINKNPLDFFFSGYIR"
     gene            2574..3872
                     /gene="miaB"
                     /locus_tag="PLO58_00315"
     CDS             2574..3872
                     /gene="miaB"
                     /locus_tag="PLO58_00315"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQO73266.1"
                     /translation="MEARKFYIETYGCQMNEADSELVIGLLRGANYQLTAIPEEADLI
                     LINSCSVRENAEKRAIARLAQFKSLKKKKPELLLGLIGCVAQRNYDEILTENKFVDLV
                     LGPDSYRNLPEYLNQEQMPIVDVNLSRTEVYDELIPSRNSAVNAWVSIMRGCNKFCTF
                     CIVPYVRGRERSRSPQSIIDEIRQALNDGYREVTLLGQNVNSYHYEAYRFPELLEVVA
                     AVPDLRRIRFTSPHPGDVDEKMLAVMQSHSNICKQIHLPLQAGSSRILKLMNRSYDQV
                     HYLKVVDLIREFLPQVALTTDIIVGFPGETADDFEQTLQVMQTIKFDSAFMFKYSPRP
                     GTKAAKMNDDVPEEEKSRRLKAVIQLQKSHTLERNKALVGTVQEVLIDGTSKKNPDEM
                     IGRTESNKIVVIKEGHPKIGEFCNVQIEKAMGVSLFGKII"
     gene            3887..4213
                     /locus_tag="PLO58_00320"
     CDS             3887..4213
                     /locus_tag="PLO58_00320"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HQO73267.1"
                     /translation="MKFLRWVVTLLIMFLIVFFVIQNSNLNPPVKIQFFWKASPVEMD
                     ILILVFLTFILGLVIGFLIAGIKILTTKNELRALSNDYKKLKKEVDLLRNQGIDETEA
                     DNSKTE"
     gene            4226..5374
                     /locus_tag="PLO58_00325"
     CDS             4226..5374
                     /locus_tag="PLO58_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73268.1"
                     /translation="MSLLDILLTLVLIALIGFIVINLTGRRRLSRSKDSASLYAMALN
                     HMLHGEDDVAIRLFRDVIRADTDNIDAYINLGILMRKNGKPKNAIKIHESLLYRQEIS
                     NIQRLEILRNLVEDYIAAGDKIRALNYVENILSIDKKNIWALEKNHILNRDLGRWEKA
                     SEYLAMALELKKERNDRLLALYKVQEGLVKYQAGEYHEARLIFRKALKIDPNCEAAYY
                     YIGKSYVLDHREEDAVEEWVKFADVAPHKAYLIFKPLQTILFNLGNFGNIESFYENLL
                     RRIPDDPKTLIALADFYEKKGNVKKAIAILEDLVEKNPGLSIAKINLAKLLVGQAELN
                     RATAILNDIIEKMVDQKEYICSNCGNRSSDILWICPVCGLADTYFTDV"
     gene            5371..6213
                     /locus_tag="PLO58_00330"
     CDS             5371..6213
                     /locus_tag="PLO58_00330"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQO73269.1"
                     /translation="MKRFVFLILIPAFIFAQNIGELKQKIRAGEVDSVRILLPALIAE
                     YPDNPELLYISGMIETDGEAALLIYRDVLSRFPSSSVADDALLKIIEYLFTKGLYTKT
                     EKYASELIKTYPQSDLIDKAIYLRLCSFNALNKRDSVDYYYKYYSTQYPNLNFHFEDY
                     KIASKVTSIESKKQSQPTSSQISSNAKSAETQTYSLPKVEVPGQGNFTLQMGVFGDPA
                     NARALRSKLEKLGYSVTLRQIERSGRKLTAVLAGSFKSEEEARAVGNKLKKDHNLDFI
                     VVKQ"
     gene            6218..6310
                     /locus_tag="PLO58_00335"
     tRNA            6218..6310
                     /locus_tag="PLO58_00335"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6253..6255,aa:Sec,seq:tca)
     regulatory      6373..6475
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6465..8840
                     /locus_tag="PLO58_00340"
     CDS             6465..8840
                     /locus_tag="PLO58_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO73270.1"
                     /translation="MHQKNLPVFFFILFILILTEANAFGEEHLIFGKLVSETTGQPVE
                     SANILLEDGSGTFSDKDGNFRIKTSFLPIKVTVTHIAFRDTSFSATSEDCGTIYLKPT
                     VITGEYVFVTATRAVKGKTPVAFSELSIEEIKSRYTVEDVPMILAIEPGVYSYSESGN
                     GTGYSYVQIRGFDQSRIAVMLNNVPLNDNESHQVYWVDHGDILSDAADVQIQRGIGNS
                     LYGSSAFGGSINVLTTIGTDRPTFSIDLGGGSYNTSKIRLGASSGQLHNGKIRLSARL
                     SQIESDGYREYHETFQRSGSAGIEYNGQNTSHQFRALVGYQKSNLMWDGVPAELINDR
                     IGRRSGYKSYIDNFLQQVYSLNSTWRISENLNFNNVAYLVKGDGYYESEKSAAYNLAV
                     DDATARDEFRDFLRSFYLDQYYPTDADSQSLELTRRKWIVNSYYGIVPTLTFIKAKYR
                     LDIGGEFKYYNGNHFGEISRFSDQNLIAQIGKGWYRYYRYIGEKSIATGFVHFTLEPL
                     KKLKLITDLQYQHLQWNLDQKKIGYAIGYDISAKWDFLNPRFGAIYELSNDFSIFANY
                     GRSQKEPADEQIIEADDYWSEPQKAAAELIDDYELGANYRGRKLDVDLNLYRINYKNE
                     QLKNIDIEQEGEYVYTQASATIHEGLEWEVRYQFNPLWALTLNGTFSRHVFESGSYKG
                     NTLPNIPGILFNGSLDVKPVKNLSISAIIRYVGEQYIDEANIGMNPAYWLTDVGLRYS
                     FGRVEIGAKVNNLFNRLYSTYGYGYEWGEYYAYYWPGATRNAFFSISYHLD"
     gene            complement(8934..10205)
                     /locus_tag="PLO58_00345"
     CDS             complement(8934..10205)
                     /locus_tag="PLO58_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HQO73271.1"
                     /translation="MALLFLVLIVVLILCGLPLFAAIGITGIIQFAISGIDPTVVIVE
                     MYRVASAPTLLAIPLFTLAGYVLAESSAPRRLLNLAEALLGWIPGGVAIGSLVTCALF
                     TCFTGASGITIIALGGLILPILLKNNYSEKFALGLITSSGSLGLLFPPSLPLILYGVV
                     ASVSIDQLFIAGLIPGILLIILLSIYAVRNDVLKKSERIPFSWQNLGRALKETVWELP
                     IPVIILGGIYGGFITAVEASAVTAFYVLIAEFILNRDLSFRRDLIRIMRDSVQLVGGI
                     LIILSCSMGATAYLIDAEIPQKALLWAQQVFSSKITFLLVLNIFLLITGMMLDIFSAI
                     IIVVPLIVPIALSYGINPVHLGIIFLTNLEIGYLTPPIGLNLFISSYRFDKSITELYR
                     ISLPFLFIMLIALVLITYIPDLSLFLVGLIQ"
     gene            complement(10205..10699)
                     /locus_tag="PLO58_00350"
     CDS             complement(10205..10699)
                     /locus_tag="PLO58_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HQO73272.1"
                     /translation="MNIALSMLNKIRTIIEKFVTALAVVLMLFLVVMAFLQVILRNFF
                     AIGLNVVDELMRNGVLWIAFCGAALTTLRSKHISIDILPRIVKGKTRLVLNWILNLTA
                     AAICLCLSWYAWQFLRLEISMNSMIAGVIPAWIIEIILPVGFFLLALTFLLRLMDKSQ
                     NLEN"
     gene            complement(10671..11675)
                     /gene="dctP"
                     /locus_tag="PLO58_00355"
     CDS             complement(10671..11675)
                     /gene="dctP"
                     /locus_tag="PLO58_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HQO73273.1"
                     /translation="MKYINAVILIILCTIPNLVAQKVHTIKVATVAPEGSSWMRQFRQ
                     FEKEVDALTNGQVKFRVYASGVQGGEKDILRKMQLGQLDVGTFTGVGLGEIVPEVRVF
                     DLPFLFRNSAEVDYVCDALFDEFQTKFLEKGFFLAGWAEVGFVYLFTNTPVTRLADFK
                     NVKMWLWKGDPLAKATFDEMKIAAIPLDITDVHTGLQTGLIDGVYISPYGALAMQWHT
                     KTKYMLNYPLTNSIGAILITTKKMNTIPEDLRNILIKKTKENTRQIVLAGRQENLESI
                     QVLKENGMQLTNLKSDAATEFIEAGVRTRDRLAGVLYSREMLDRVLALLDEYRAEHAE
                     "
     gene            complement(11689..12600)
                     /locus_tag="PLO58_00360"
     CDS             complement(11689..12600)
                     /locus_tag="PLO58_00360"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HQO73274.1"
                     /translation="MSSKNSIPAIKFLSFTLILCLGGLSCSPQRFLFNKVSNVFDSGI
                     EIIYQEGDLELAERFLSSNLETIVILVAQDPENPRLNLMAAQAFGAYAMAFVEDKDPQ
                     RAGLLYVRGLEYAFKSLPPQIRFSRNITPADLEKLLVESRKSDVPQLFWVGYNWGMYI
                     LQNLDQPRLLGDLAKVELLMRRVAELDAGYNFGGVDIFYGTFYSARPPMLGGNPELGR
                     SYYLKNIERNPNFLMTKLYYARYYAVQVQDKELFDNLLTEIETFDLESAPEIRLFNAL
                     AKQKATNLRQNAELFLDLNEILETNER"
     gene            12662..13231
                     /locus_tag="PLO58_00365"
     CDS             12662..13231
                     /locus_tag="PLO58_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQO73275.1"
                     /translation="MVLSITMKQMVKTILLRSIWLGLGVIVIGLIFNWLSPNGIPLVA
                     KYQTVTVRGKSQKIPLFMNRKAQTNQETVFEISPVSELDTEKAWQFFTNGGTIFIDTR
                     EYEDYLAGHIAGAISIPLSEFQNDPTIVEELDRQVRIVTYCDGSDCQSSIDMAVRLNE
                     MGFEQVWFYFGGWKEWVEADLPIVKGSKP"
     gene            13228..13671
                     /locus_tag="PLO58_00370"
     CDS             13228..13671
                     /locus_tag="PLO58_00370"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HQO73276.1"
                     /translation="MKSKFWDWLELFIRLVIGFLFIYAAVGKIIHPEKFAVIVYNYRV
                     LPVELVNITAILVPWLEIALGVTLILGIWLETAAFLLSILIIVFIGLIISAIIRGLNI
                     ECGCFTLSKTGSLVSWKRVAEDLLILAGSLFIFFRHLPQEQSTTE"
     gene            complement(13768..14733)
                     /locus_tag="PLO58_00375"
     CDS             complement(13768..14733)
                     /locus_tag="PLO58_00375"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO73277.1"
                     /translation="MQNISKYRILQNILLLFSLASFVFADEVWLLINPETRYASLAGA
                     ATAIVNDVAALYYNPAGLANGDELSASANYGTWLGNSEIYYAGAVRNFKHRGGLGTSL
                     YLYNLDAKITGDDYTATDFSYNFIYVSIGYGCNFTRKITLGAATKFIYTNAPNTASDG
                     IQSINGKGLACDLGIQINNLLPFLTIGENKTGGLSLGAAFCNLGPAIKYRNDFTNYKS
                     KLVPRLRLGGGWRLLNKDLISATISYDFIEDYYDSNSSNHLKSHLIGGAFSFYKRLYI
                     HCGQHIENNENYITWGVAYNLDKFIIGYCRYPSQKSNFFSVSYSL"
     gene            15001..16440
                     /gene="gltX"
                     /locus_tag="PLO58_00380"
     CDS             15001..16440
                     /gene="gltX"
                     /locus_tag="PLO58_00380"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQO73278.1"
                     /translation="MTENTRVRFAPSPTGSLHLGGARTAIYNWLFARSVGGKFLLRIE
                     DTDVERSRKELVNQICDSLQWLGLNWDEEPVSQSQRLELYQQKVTELLKAGKAYRCFC
                     SSEELEAEKAATIKQNKAYRYSGKCRSLSLQQSEELMAQNKPYTVRIKLEPGVIEWDD
                     LVYGHIAVNNNELDDFIIQRSNGVPVYQMAVVVDDIDMRITHIIRGEDHIPNTPKQIN
                     LFRAFGQPIPQFAHLPLLLGPDNKRLSKRHGATGVDEYREQGFPAEAVVNYLALLGWA
                     PQNDQEVLSIDEIIQQFQLTAISRKPAVFDIKKLEWISGEHLNRMPVAKIRSVLVPKL
                     IAAGLINQQESENRTQYVDSVIELLRGRVRTYNQYLEWGCYFFQDPENYDEKAAAKYW
                     HTPETNEHLAKLSQELATLTDFSANTIEETLRRTAEHAGLKAADLIHPLRLALTGFGI
                     SPGIFQVAEILGKETVLRRIQKAIEYFKT"
     gene            16450..17712
                     /locus_tag="PLO58_00385"
     CDS             16450..17712
                     /locus_tag="PLO58_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73279.1"
                     /translation="MKNGWQIAYFLMLSFLLFGLVGAQTPNSHPINLFILTFTNYEGA
                     PEIDWWKEGFVDFLMDYFSNNPDLNVHRINDPATIPNQIKNLSETEITRCYLLTGNYK
                     IIDQKFEVDLELIDINTWRLLQKKNISIQSADMAKIIESVNHGATEVLTPYLIKKAET
                     TVAQEISSPSQPTKNNYQKAVAASVAISSVIDKFSETHPESAQHEPSFGGTVTNELSL
                     KFGEAIAQTGSFQYTINQIIRNPYKLEISDPLFRRLSANDEIINVTFFINYVLSRDLI
                     QDMLTTFPYEIQTKNDEYTEYIFSGKRFAYSEDFIKAIARGDYRTHPVYAFITNTETV
                     ACVLIDMPIMYRKNLISSSKVQFTAKYQPLINVTANGWNVVIQLKNTDARAEYNFDIS
                     FEQLKNLSRIDVMFLSEADIQEYLKIMN"
     gene            17860..18228
                     /locus_tag="PLO58_00390"
     CDS             17860..18228
                     /locus_tag="PLO58_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQO73280.1"
                     /translation="MNQYKILIVDDEQNVCEFLGEFLQDKGYLVIKARSGSKALRYLE
                     KNNPDLVLLDILMPGMSGLEVLKRIRKLYPDLPVIILTGVKDKQVVDDIINVGPVDFI
                     PKPIDLDVLEKCISSNLAKK"
     gene            18239..19474
                     /locus_tag="PLO58_00395"
     CDS             18239..19474
                     /locus_tag="PLO58_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQO73281.1"
                     /translation="MDTAILFLPERMASNKANSIFNTIRGILHTQLNLNIVIQNLQNS
                     QPIYEQITSIKNALVFASIYDSIYLEKFENFQPLFKIKTKSSDNILVIPQKATVRHLK
                     ELAGGKVLITKGIDHNCVNPVILYLIKNCGIFFAPKSLSIYYEPKTLLDQLLKGDVSA
                     ILTNTFQFDLWEADEQQSLKILGKCPEESEIVALIGPKFNVRKLKKNFNDWLQEHGSD
                     YFKDGYKLQSIDKYENILLIEAIEGLGYKLKDFIENYPDLIIKNIKSKLAEEVETLNE
                     KYSGLQVFNEKLVDMYKEIRESHDRLYQEIDSAVQNQILFLKDGTIIGISRGFMNWVQ
                     KSRPEIIGKNINDFINPNINKPFNELIQQVDYGLIKSFGIRLKNTNDNDTTAKMDFTI
                     LELRDSKVVMGIVSMITDR"
     gene            19498..21663
                     /locus_tag="PLO58_00400"
     CDS             19498..21663
                     /locus_tag="PLO58_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HQO73282.1"
                     /translation="MKKYIVSLAFLLLCNLAIAQNQMATVLKVAGDVKLRTKGRSDFS
                     RKVTMGMGINTGDALKTGSDGFVAIIFTEDKSLLKIRNDSEIELIKDNLGDRTSKVTQ
                     GKVLAEITPGIKTSFRIETPTSVASVKGTKFWTITIPDFGDKFYGVEGQVGILNLITG
                     LEADLEPGKMVVSTIDGELFSLPVDPDEIPVDPEEMKPEEEPGEPSGMKMEEETEMPA
                     MGAIEATGQPPAESVAEAGKKEEEAQKAKPFGMGLGLGSVTIGGKIYNQIALRPEFKF
                     GKLGVSLDIAFYMDENGNIRKEEWDEFIDYIDKIYYVRWAQQGDPFFIKVGALDNVSM
                     GYGILLNGYSNTTEYPQVRKVGIHTGMQWSKIGWEAYIANLKEITGPGLMAGRLTYRP
                     ISKFPLVLGTTVVADVYPYKGLPDADEDDVADALDLYPEADDNVVLDSLKTGFTLGQR
                     EFLRNNGFNIPSETIIQNGITKLSDYDRLLNGAVSVDVGVPILNKKSFNLTVYGQAAS
                     FFPVEDSAVVYNSATQSYDRVAFTPGYGFAVPGVKMGIARIANLTLEYRYAAENFLFN
                     YWDRAYDYERVQIRNNQIYTKQQMSLMQSSMQGVYGAVDVNIMNYFILGGYYQHMFSE
                     GTEIKSFMASASIPKGIVPKLADATAFYQRNNDENPFDFKNPSENTILGYRIGFELGG
                     GAIIYYKFQRTYRDFDGNGIVDPDLEAVNLTTIETGFSF"
     assembly_gap    21664..21763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21764..22381
                     /locus_tag="PLO58_00405"
     CDS             <21764..22381
                     /locus_tag="PLO58_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73283.1"
                     /translation="GYQEQLLYFTPYQMYLIASRISVREPLTDESRQLIQKKYKPLYA
                     EKFLAEIPMNSYCRLKLKDKSQFDAVYYRLKGDSLLFWIDRQVVPINRNDLVKLKYWD
                     NYRPNNWVKWGTMGSVAVISYFGSGLVADWLNITTKNAILTQFAAASVGCVIGYKISP
                     IVNEYIMPSTVIKFPTGNIKRLDTIARMCYNIRNIKDKIWQIITP"
     gene            22360..22830
                     /locus_tag="PLO58_00410"
     CDS             22360..22830
                     /locus_tag="PLO58_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73284.1"
                     /translation="MANYYTLNKNLSTRRTTSPTEETPPSPAVKIDFSDTEQRYEYIR
                     SKLDDIMENIGQCYSERLTKELLHRLEKTILDFHTEVIALLDKLEKLEIERNQLPERT
                     PVSESVSTPVESKSGERAKRIEESEQRKAGIVIESELNKKTESEPKRRFFNRRK"
     gene            23050..23122
                     /locus_tag="PLO58_00415"
     tRNA            23050..23122
                     /locus_tag="PLO58_00415"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23083..23085,aa:Ala,seq:cgc)
     gene            23152..23418
                     /gene="ffs"
                     /locus_tag="PLO58_00420"
     ncRNA           23152..23418
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLO58_00420"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            23430..25082
                     /gene="dnaX"
                     /locus_tag="PLO58_00425"
     CDS             23430..25082
                     /gene="dnaX"
                     /locus_tag="PLO58_00425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQO73285.1"
                     /translation="MNYKVISRKYRPQNFNEIIGQDHICTTLQNALKSGRIAHAYLFT
                     GSRGIGKTSTARILAKALNCLNPQDYNPCNVCQNCVEITNSRSLDVIEIDGASNRGID
                     QVRELRENVKYPPSNSKYRIFIIDEVHMLTREAFNALLKTLEEPPPHVVFIFATTEPL
                     KVPPTIISRCQRYDFHRIPVREIVGQLKKIAESENLTVSDDILTLLAKKSEGAMRDAE
                     SLLEQVVSFSSENVNLNSIQKLLGLTDYEYYFTISNQVFEHDLNALIKSAEEVFNNGV
                     NISEFLIGLSEHFRNLLITAIVKSAADLDLPDSLKNHYQEEAQRWSVNDLMRLLKLVG
                     EAQAGLKAAMNQRTHLEFALLRMGAMEKTVTIQSILQNIDARQSGGIGQIKPVAQTMD
                     FFSTKPVVPKTVPHPDSNVIKEKVSDNYIPEKKSEPISKSFDSLVTFEAIQAQWSTII
                     LKLEESNLSLAEFLKVSKPMQLNGNVLDLGFTSEQDFAHKNVAKRAPTIEQILIGFFH
                     KPIKIHCIQIESAAESADTVKKPIDEMTEKVVDMFDGEIIIT"
     gene            25092..25430
                     /locus_tag="PLO58_00430"
     CDS             25092..25430
                     /locus_tag="PLO58_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011866032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQO73286.1"
                     /translation="MIPKGGLNNLMKQFSKVQAEMERVQEELANLTVEGSAGGGMVKV
                     VANGKQEIQSIKIDPEILKDDVEMVEDMIVAAVNQALARAQELSQEKMADLTGGMLSN
                     LPGGMKIPGL"
     gene            25427..26020
                     /gene="recR"
                     /locus_tag="PLO58_00435"
     CDS             25427..26020
                     /gene="recR"
                     /locus_tag="PLO58_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQO73287.1"
                     /translation="MSALPDSLQNLIERFARFPGIGRKSAQRLAFYLLKADREEAVAL
                     AKAIIDVKDRIYKCSQCYNISETDPCKICTDEKRDHTTICVVQEPMDVFSIEKTGHYR
                     GLYHVLGGVLSPLNGIGPDDLNIQGLLYRLANVKEVILAINLTRDGETTALYLSKLLK
                     SKNVKVSRIAQGLPAGIELEYADEITLTHALEGRTSF"
     gene            26022..26621
                     /gene="pgsA"
                     /locus_tag="PLO58_00440"
     CDS             26022..26621
                     /gene="pgsA"
                     /locus_tag="PLO58_00440"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQO73288.1"
                     /translation="MKKLFNWPNLLTFFRILLTPLFLYFLFGNQPYFEIFALLIFIIA
                     SVTDIYDGYIARKYASVTKLGKFLDPLADKILMSAAFISFVILDLVPLWMVILVILRD
                     FIVTGIRILMNSRHLTMETRKSAKTKTGVQVGVVCFILAYLITQRWEIFLGISDYVRL
                     IEEYRIIYILMLITTIITIWTGVEYILVNKDKIQQALKK"
     gene            26618..27097
                     /locus_tag="PLO58_00445"
     CDS             26618..27097
                     /locus_tag="PLO58_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HQO73289.1"
                     /translation="MNITGAKKLLSRIIASVCFIGYLPFAPGTWGTVAGLVVWFLLPW
                     HSFWFDLILVTVTFFIGIFTSEYIEKQIGEMDPAYIVIDEVVGVWLALAIIPTLTYPK
                     DLALIVLVFLIFRIFDIKKFKPIRKLELIGGGFGIMADDILAGIFTGIIVNLGILIL"
     gene            27094..28323
                     /locus_tag="PLO58_00450"
     CDS             27094..28323
                     /locus_tag="PLO58_00450"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HQO73290.1"
                     /translation="MTNAAIISIGNELISGLVNNTNSAYIARTLNNFGIVVSLIVTVG
                     DEQKHIWTVLDNLPPEINLVMITGGLGPTNDDITVSALARYFNLPLVFNKETASNLVK
                     HFAKRGLKMPEINLKQAYLPKEAVILDNPVGSAPGLKIEHQNRVYYVLPGVPREVEAI
                     MTKIIAPQLAALGHKAIVKKYLHITGIPESQIYTLLKEWIEQHSHVKVAFLPQTAHID
                     LELTTSEPQKSVRIDEYVKVITEILGDYVFGFDDDTLESVIAHLLLQKHLTVAVAESC
                     TGGLIASRLTDVPGSSNYFKTGIVAYSNATKINLLGVKPEIIEKYGAVSSEVANQMAT
                     RVRQISGCDIGLSSTGIAGPSGGSDAKPVGLVWIGVSDKNATRTFQYQFSGDRSTNKL
                     RFSQAALNQLRLYLIGK"
     gene            28327..28884
                     /gene="thpR"
                     /locus_tag="PLO58_00455"
     CDS             28327..28884
                     /gene="thpR"
                     /locus_tag="PLO58_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HQO73291.1"
                     /translation="MKRTFIAIPVSQETCLQVDTIQRTIPELRENTRLVPTNNLHLTL
                     KFLGDTDEKVIPTISQALDSSITTIDRFEFIWEGLGCFPNNHKPRVLWLGVGQGLEQI
                     QKLTLAIESVLENFGYQPEKREFRPHLTIGRVKNPFKKVAGLESFLNYKINPTINPVK
                     DVIFFESNLTMGGPIYTRLAVFNLK"
     gene            28900..29907
                     /gene="recA"
                     /locus_tag="PLO58_00460"
     CDS             28900..29907
                     /gene="recA"
                     /locus_tag="PLO58_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQO73292.1"
                     /translation="MAEKNDDKIKALDLAITQIDRQFGKGSIMRLGDDSMKITVDVIP
                     TGCLSLDACIGVGGIPRGRITEIFGPESSGKTTLALHIIAQAQKTGGYAAFIDAEHAL
                     DPQYAAKLGVNTRDLLISQPDHGEQALEITETLVRSNAIDVIVIDSVAALVPRAELEG
                     EMGDSHVGLQARLMSQALRKLTGAVSKSNTAVIFINQIREKIGVTYGNPETTTGGRAL
                     KFYTSVRLEIRRISTIKEGNTNVGSRTRVKVVKNKMAPPFKETEFDLTFDQGISYVGD
                     ILDTAVEAGFVQKSGAWYSYGDERIGQGRESAKGYLTENKELLTKLEQQVKTYLGLIK
                     E"
     gene            29935..30588
                     /locus_tag="PLO58_00465"
     CDS             29935..30588
                     /locus_tag="PLO58_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HQO73293.1"
                     /translation="MSSDFSSEAEILKVVTKIESQKKKSARINIYLDNEFAFGISAET
                     LNLFRICVGQQLTEKQINMIQQHELFEQAKEAAIRYLGLRIRSQRELELYLTRRQKYP
                     LTVASRVIKYCTERGYLDDKVFCETFIRDQLELNHNGIRKIRQALVAKGIDREVIDEA
                     IKNLVKDEDQLKAAWIIGQKKASTLKNDPKRRDKLYRYLIQKGYKPDVVIKVLQKIL"
     gene            30734..>32799
                     /gene="alaS"
                     /locus_tag="PLO58_00470"
     CDS             30734..>32799
                     /gene="alaS"
                     /locus_tag="PLO58_00470"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQO73294.1"
                     /translation="MKTANDVRREFLEFFASHGHKIVRSAPVIPQSDPTLLFTNAGMN
                     QFKGIFLGQEKIESPRIANSQKCIRVSGKHNDLEEVGKDTYHHTFFEMLGNWSFGDYY
                     KAEAVQMAWELLTEVWKLPTERLWVTIYQDDEEALQAWQRIAGLDQSRVLRFGEKDNF
                     WEMGETGPCGPCSEIHYYTGPHPEHQQAEMINSGNPEYIELWNLVFIQFDRDKSGELK
                     PLPLKHVDTGAGLERMVAVLQGKKSNYDTDLFKPIIDRIVSLTGKEYHTENGVPHRVI
                     ADHVRMLTFAIADGGLPSNDGRGYVIRRILRRAARFGRMLEMHKPFIYQLVDSVIEVL
                     GATYPEIAERRNHIQAVIKAEEKSFGLTLDRGLEVFAKLKENLQRNNIRIIPGEEVFK
                     LYDTFGFPMDLTRLLAEEAGLSIDEKGFENLMNRQKERARASVVFQADDFLENEDWQT
                     LTTGDDSEFVGYENLETQSVIRKYLIEGQKVHLILDRTPFYAEAGGEVGDVGIIEGNN
                     FRIRIDNTRKIGSSIIHSGHFEKGNSIVDPIVKACVDNVANLQIRANHTATHLLQAAL
                     RQILGEHVHQSGSLVTPEKLRFDFTHFTKMTKSEIEAVEDWVNARIRENLPVNAEVKD
                     YQSARESGAMALFGEKYGDVVRVINVGDYSKELCGGKHVNRTGDIGCFKIISETAIAA
                     GVRRIEA"
     assembly_gap    32800..32899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32900..33193
                     /locus_tag="PLO58_00475"
     CDS             <32900..33193
                     /locus_tag="PLO58_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HQO73295.1"
                     /translation="ELKEIGDLVRAKLHNGVGVIAGIIDEKPNITVVITDHAIKRYGL
                     KAGDIVRELGKILGGGGGGQAHLATAGGRFTDKIPEALQAVIPLIKSKIESKN"
     gene            33206..34651
                     /gene="gcvPB"
                     /locus_tag="PLO58_00480"
     CDS             33206..34651
                     /gene="gcvPB"
                     /locus_tag="PLO58_00480"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HQO73296.1"
                     /translation="MEKLIYEYSQSGKEGVSLPQGIESAVSPAELIPAELLRQSELQL
                     PEVSEPEVVRHFTNLSTMNHHVDKDLYPLGSCTMKYNPKVNDRSAALSGLANCHPLQD
                     VGTVQGALQIIYELERYLCEITGLQAATLQPAAGSHGELTGVLLMKKYFDQKGENRQF
                     ILIPDSAHGTNPASAASANYKTISLKSNQRGCIDLEDLRAKMNPSVAGLMLTNPNTLG
                     LFEEEVAEINAIVHNQGGLVYMDGANLNALLGIVRPGDMGFDITHINLHKTFATPHGG
                     GGPGSGAIAVNEKLVEFLPVPRIEKNGDRFSLNYCYPRTIGKVLGFYGNFGIIVRAYC
                     YIRMMGTKGLQAVSRNAILNANYLRQKLSSVFEIPYNRTCMHEFVASGERQLQRSGIK
                     TLDIAKRLLDYGFHSPTVYFPLIVKEALMIEPTETESKETLDRFIEALVKIDQEATNE
                     PEKLRNAPWTTPVRRLDELKANKELNICWGK"
     gene            34636..35037
                     /locus_tag="PLO58_00485"
     CDS             34636..35037
                     /locus_tag="PLO58_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQO73297.1"
                     /translation="MLGEVERQIVNTTDAPRAIGPYSQAVISGNFVFTAGQLGLDPVA
                     GKIVSNDFEQQVRQIMRNLKAILQAAESDLKNVVKFTVFVTDLDNFGLLNQVFGEYFP
                     ESAPARSAVQVSRLPLDGLVEIEAIAVKRGK"
     gene            35038..35922
                     /locus_tag="PLO58_00490"
     CDS             35038..35922
                     /locus_tag="PLO58_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HQO73298.1"
                     /translation="MPYKFAVIVNPVAGKGKTVHNLPLLKEIAAKTKSRFDYFITEGP
                     LHATKIADRIHQEYDAVVAYGGDGTANEVMNGLAGTETPIGVIPEGTGNDFARCINVP
                     HKFERAIQILDEYKTRLMDLGTIGNRVFINGVGVGFDAYVNVKTKSLKMLKGSMAYLY
                     SVLSSLAFWKSLPLAIEIDGCPISTDRSFLVAVGNGWACGGGLKLNPQANIHDSLLDV
                     CLIPEMPVWKLFLNLRRLKDGTIAEVPGVLTMRCQAINISSEKPLPVHFDGEIFDAGA
                     GEVDIKIVPQSASVVGAW"
     gene            36022..36459
                     /locus_tag="PLO58_00495"
     CDS             36022..36459
                     /locus_tag="PLO58_00495"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HQO73299.1"
                     /translation="MKISPKGAFIRSAIIPGWGQVYNKKPVKGVVLLSAEGYFLYNFF
                     YYNRIYDYVKTTKETLGVETWVGLTEEEKKAQVLAVTGYELTLNSWRPREKRNKYGWW
                     SLGTYIFCLLDAVVDAHLINFPKGEIELGSTEIGLNLSVRVNF"
     gene            36461..>36819
                     /locus_tag="PLO58_00500"
     CDS             36461..>36819
                     /locus_tag="PLO58_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HQO73300.1"
                     /translation="MTKSREQWGSKIGFVLAAAGSAVGLGNIWRFPYLVGENGGAVFI
                     FIYLICVAVIGLPVIISEVIIGRNAQKDPVGSFKKLSNNNPFWKGVGFLGVFTGFIIL
                     SYYNVIAGWCLGYIVEAV"
     assembly_gap    36820..36919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <36920..37618
                     /locus_tag="PLO58_00505"
     CDS             <36920..37618
                     /locus_tag="PLO58_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HQO73301.1"
                     /translation="VALGQAFFSLSIGMGVMITYGSYLSKENNIPSQATRIVVIDTSV
                     SLLAGFMIFPAIFALGLKPHQGPSLLFNTAPILFNYIPGGYWFRILFFILLALAALTS
                     TISLLEVITAYFVDEFKLNRKMIAIIGGAISLLLGIPSALSFGRWSSFQIMNLSIFNF
                     CDYLASNICLPIGGLFITIFVGWFWGKNNALLEMRQGADRTPNWFFSVWRFLIRYVAP
                     VLIAMVLLSSLGII"
     gene            37615..37974
                     /locus_tag="PLO58_00510"
     CDS             37615..37974
                     /locus_tag="PLO58_00510"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HQO73302.1"
                     /translation="MKNWWLLLLIFAIGGRLLAVPPVGEIIKSKPDDEWFAMDKLKHF
                     STSFYLTTTLFYSQNRIFDLKTDQANRNAAGLTISLGLLKEIRDLKQKENYFSWRDLA
                     ADILGAGAAILFINGIE"
     gene            37971..>38218
                     /locus_tag="PLO58_00515"
     CDS             37971..>38218
                     /locus_tag="PLO58_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQO73303.1"
                     /translation="MKTLNEYIGCIHIHTVYSDGSGTYEEVIRAAQEAGLDYIMVSDH
                     QTLRGRDEGYAGWHANLFVSIGYEIQDLEDRHHYLVFG"
     assembly_gap    38219..38318
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            38431..39795
                     /gene="purF"
                     /locus_tag="PLO58_00520"
     CDS             38431..39795
                     /gene="purF"
                     /locus_tag="PLO58_00520"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQO73304.1"
                     /translation="MCGIVGIYNHEEAATLTYLGLYALQHRGQEGAGIASFDGKVSHL
                     QKGKGLVADVFINREQLMHLSGKTAIGHVRYSTYGSNGNENIQPLAFKYKGSQISIVH
                     NGNLINLDEIGRDLESHGALFQTTSDTEYIIHLFAHSGGDSLVEKFLQVFRRIKGAFS
                     VLILFDDKMIVARDHRGFRPLCIGTLDGSYIFASETCALDLIGATYERDVKPGEMIVL
                     DGTNVKSYQIAPPEPKHCIFEYVYFSRPDSQIFGEYVDKTRRKLGKILAMEKPAPEAE
                     IVISVPDSSNTIALGYSQRSTARFEIALIRNHYIGRTFIHPTQTMRDFTAKIKFNPVG
                     GVLKDRKIVVVDDSIVRGTTLRKLARMLRSAGAKEIHIRIGSPPVKFPCYYGMDFPSH
                     EELIANQKSITEIKDYLEVDSLEYISPEGLLNAMSLPAENFCTACFTGHYCEKINQEK
                     ENSC"
     gene            39789..40754
                     /locus_tag="PLO58_00525"
     CDS             39789..40754
                     /locus_tag="PLO58_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HQO73305.1"
                     /translation="MLKVPSKSIAWLDYLFIFRPTLFFAVWIITLAGYSGYFIFSEKQ
                     PAWWSLEVNWKLVLGFIVVTMATGATFILNQLHDIESDKENRKLFLISEEHIKADLAL
                     KIALITIGVSLVLALIINWQFFIALGICVFFWGYLYNYKPFEWKDKPMLGVLVNFISG
                     LCLFICGWIIAGKWQLKDIIYAIPYLLAWTAVAVLTTIPDKEGDLKTGKKTISVHYEN
                     RVTIWLAFICLVLSFALGMYFNDPVISLSCLLSSPLFIIMLFKPTEAWVLRSIRYPIL
                     FLALILCVEFPLFFIVLLINYYLCRFYYLSRFNLNYPTFQVEESV"
     gene            40751..40924
                     /locus_tag="PLO58_00530"
     CDS             40751..40924
                     /locus_tag="PLO58_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQO73306.1"
                     /translation="MIEVNPDCCDLCGTCISVCPKDCITLTESRLIVDDSQCNACQIC
                     VRVCPFKALRLVE"
     gene            40933..42111
                     /locus_tag="PLO58_00535"
     CDS             40933..42111
                     /locus_tag="PLO58_00535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQO73307.1"
                     /translation="MRKEYDVIVVGGGPAGSTAARFAKTATNTVALFERDREIGIPVR
                     CAEATSIPDLERFVKIDPHWIATTITKARLISPSDQKVWLNLPNDGVILNRRLFDNGL
                     AEIAATQGVEIFTKANVYKIVSDGEQHWEVFVRHLDRDYIVKSKIIIAADGVESRIAR
                     FAGIRTQTVLKDIESCAQVLLGNLDVAENQIDFYFSSRWAPGGYAWVFPKGNHTANVG
                     LGVNGAYYKGRSAIEYLDDFINEKFPEAARLTTVAGGVPVAKSLKKIASDGLLIVGDA
                     ARQVNPVTGGGILAAITAGKIAGLVAAQAIARNDWSVNVLKEYQTEWDKNVGKDYARF
                     YKIKEWMTELTDDELDEIATLLQKIPPDDINIMTVFKIALRNKPALLLEAIKLFAQMI
                     "
     gene            42111..44978
                     /locus_tag="PLO58_00540"
     CDS             42111..44978
                     /locus_tag="PLO58_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HQO73308.1"
                     /translation="MDDFLTLAGLNEFIALDVETTGLNPQQDAVIEFAAVKFINGKPS
                     NTLNFLCNPGFPISPEIEQLTGIKDEMLKDKLPFEQHLDEVLEFIGRIPLVGHNIGFD
                     LGFLKEYLKNFKSQRKRILYNPLYDTLLLAQAFLFFLYNHRLSTIAEFYGLSAKGAHR
                     AENDALNTGLIFVKLIEEVQSYDLETIQTINMVLKDTDDPNKLLYKNAAEALLLQRSL
                     GSEEAPQIGWDPPVNIFGQSIEKNKESAKASLKIEKFFEPAGPIAQNFVGFEDRPQQK
                     EMAQLVLNSLQAGDSAIIEAGTGVGKSLAYLIPAVVWLNQQKKEKKRVVIASNTKTLQ
                     EQIFYKEIPFVSEKLKLPFKAVLLKGRSNYICLTRWHRYLNALPQQLHLAARSAIIPI
                     IIWLKHTRTGDIAENNGFNLAAHYYIWNEICSEPGYCTTSVCQKYGGCFLGKIRYHAM
                     NADIMVVNHSLLLADSAADNQILPGFDALIIDEAHNLEKNSYNYFAQKVNLPTLIYFL
                     NGIYSGSAPERGLLVDLAESAHQLKSLDKIEPLRQMILEKTSDVKIAAEVFFRKIVAT
                     KFNPANGNQKSFGIKKRYKDFSREFPNLEIETNNLIYELGTLWNLLGNLSNKMDEIWG
                     DYAEEYDELQVRLMNQKIMVEAFLETLRKVTTANDDSHIFWYEIAPNGKNNTVEIVGT
                     PLNIASNIFEKVYKHVSSAMLTSATLQVAGAFDYVLSRTGLNLIEDHNVVSRALGSPF
                     YYNDQMAFFTYHQNDNEISEAFIAARLIKRLSSETGKGILVLFTSYNFMYEVYQLCLP
                     TFIEMGVTLLAQGQGASRTALLEQFRLEQNSVLLGTDSFWEGVDVVGPALEILVITKL
                     PFPVPTEPIIEANVEKLKAEGQDPFNEYYVPESVLKFRQGVGRLIRSINDVGVVINLD
                     SRIDKKAYGRYFKKSLPVEPISIVGEDELLAAVRKFFRIGK"
     gene            44980..>45581
                     /locus_tag="PLO58_00545"
     CDS             44980..>45581
                     /locus_tag="PLO58_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQO73309.1"
                     /translation="MRIFRICLCFLLSTSVILAENSKKLTVREIIDDPGLDARTYRQK
                     QFTWTPKGDGVLFLEKNKNQNCDLLKFSINSLKIDTVFTYEQLRWSNDTHTVQLDPFG
                     FISAPDGFRYLLTGENDLFLGDERDGTIVRLTNDGTQKSVPKFSPNGKWVSYICDHNI
                     WIVNCESRSSLQLTTDGNESIFNGESDWVYTEEFDFEQAY"
     assembly_gap    45582..45681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <45682..46980
                     /locus_tag="PLO58_00550"
     CDS             <45682..46980
                     /locus_tag="PLO58_00550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQO73310.1"
                     /translation="VVIQSINRLQNNQKVTYGNPRTGQTKVILEEKDPYWLNITDLYY
                     FMKNSRNFIYYSEIDNFMHLYLYDFEGNLIKPITSGDWMVTELNGVNEKDSLVYFTAT
                     KNSVLERHIYSVHLGSGAISQIDSGDGVHSAVFSPDLKYYLDFFSNVNSQVDVRIIQN
                     NGRKITDLYHNNDFSPQKYNFGVTRFIKIPAIDGDSLYASLLYPSDFDPQKKYPVIVY
                     VYGGPGVQVVQNRYGGAWTHLLAQQGYLIFSLDNRGSYGRGRYWERRIYQQLGKIELQ
                     DQLDGVKYLKSLPYIDSGRIGIWGASYGGYMTLYALTKTPEVFKTGIAISPVTDWKYY
                     DAPYTERYLGLPINSQNIYKESAPLNYVNQIKANFLLIHGTYDDNVHLQQSLEFIDEL
                     ISNNVRFELMLYPGRKHGITDREGQIHMYETFLEFLQRNL"
     gene            46996..47937
                     /gene="menA"
                     /locus_tag="PLO58_00555"
     CDS             46996..47937
                     /gene="menA"
                     /locus_tag="PLO58_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HQO73311.1"
                     /translation="MSANRKLSDSLKLWLRAYRAPFLTASVAPVILGTSVGWYENGRI
                     NFVSGLLALLGTIFAHFGANIFNDYYDHLTGCDDNNLDFTPFSGGSRTIQNGLLTARQ
                     AFWAGLVAFLLVVLIGLLLIVYTGRPALIIFGIVGLAIGFLYTAIPVKLAYRGLGEMG
                     IFIAFGPLIVIGASYVQTGTISFLAFLASVPAGLLVMLILYANEILDEKADRSADKNT
                     IIVKIANRNTALKIYRLILYVVPIWILVFVIVGYFPYWSLIILCLMPKYIGTIFLSKK
                     PFNDVKEMVPLSANTIQLQFLMTITLAISFILAGLTG"
     gene            47994..48064
                     /locus_tag="PLO58_00560"
     tRNA            47994..48064
                     /locus_tag="PLO58_00560"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48026..48028,aa:Gln,seq:ctg)
     gene            48095..48167
                     /locus_tag="PLO58_00565"
     tRNA            48095..48167
                     /locus_tag="PLO58_00565"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48129..48131,aa:Glu,seq:ctc)
     assembly_gap    48211..48310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(48498..49115)
                     /locus_tag="PLO58_00570"
     CDS             complement(48498..49115)
                     /locus_tag="PLO58_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HQO73312.1"
                     /translation="MNNEAIKISVIVVIAAYISAQMLSDITSLKIISLLGFSIDAGTL
                     IYPFTFTLRDLVHKTIGLKGTRIVIICAAVINLLMALLFWIVSYIPGDPNVGPQLEFA
                     KVLSPVWRIVIASIIAEVIAELLDTEIYRIWIEKVTKRHQWARVLVSNSLSIPIDSLI
                     FVWGAFGGIYNPSVVWSIFFANLILKGAITIISLPMIYLVKDKNY"
     gene            49348..>50240
                     /gene="manA"
                     /locus_tag="PLO58_00575"
     CDS             49348..>50240
                     /gene="manA"
                     /locus_tag="PLO58_00575"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HQO73313.1"
                     /translation="MENNFEIKPFVLRNKIQHYEWGTRGEQAFIPRLLGIQSEGNRPY
                     AELWIGAHPSAPSQVNISNDLIDLTELIKKHPVEILGETVFKRFGANLPFLFKVLSAG
                     KPLSIQAHPNKSQAVRLHQRDPIHYPDSNHKLEIAIAIDNLKALVGLLNIVELRKKLE
                     LYPPIQSFTDRVLPGDSTISEADETTIVKQFYSTLIRKSVGEPQLYGNLVESLAESIF
                     TRGNRISEQEQLFLELKKCYPSSDVGLILILLMNLVQLTSGQALNISAGIPHAYISGN
                     IIECMTNSDNVVRIGLTNKYK"
BASE COUNT        14666 a         9538 c        10902 g        14534 t
ORIGIN      
        1 agcatctatc gttaccgtgt gtccgttttg aattgttatg gtataatcgt tcgatgtagg
       61 agaaaaagat gcattgctcc aggtcgtttg atccgttgat gattcccagg tagaattatc
      121 tccccaattc ccagtaattt tacttcgata atgcatcggt ggactagtca cagtgccact
      181 gcaggttaca gttctatcgt cggccccctc agaagagcag gtaatatttt cggaactata
      241 tgagccaatt gccaagccgg cttttaatcg cacatagata atggtttcgt tcacggaacc
      301 gtctgattga gcaaaagtaa gcgatgaggt gaaatccgaa ccacttgttt ttgaaatttc
      361 aaagtgttct ggtgccgaaa ttgatatgtc tgccgttaga ttcgttccac taaccataaa
      421 agtctgctcg gcagatgggc cagagccaac gacataagaa aaacctgaca gactactagg
      481 atttgccgat atagtaggta cttttatttc ataagctgtt atagataaat cgtcaatagc
      541 taaaccagga ctattagttc cagtttttag ccaacgaaac catatttcat gtccgttagg
      601 tacttcaatg ataacatttg atgatacatt ttggtaatta ccttcagcat taccatcaag
      661 agcgctagag gtaccagttt tcaaagctgt aaaatctaaa tctgaaaaag atgtccatgt
      721 tccagattca attcctgttg catctacttg ataagaaaaa actaatgtat gagaattcgc
      781 cgtttgcctc cattgctccc ctttatagga aatattaaat gaagtaatag tgtttccagt
      841 gttatttact aaacgaatac caaaagcggg ggaagccgaa ctagatgaaa gtccacctaa
      901 tgctctctca ctggcatccg ccgatccata gctatgtaaa ccgccagttg tactactacc
      961 attatcggcg atgtactctg tcctgtttga ataccacccg ctgatagtgg aattatcggt
     1021 ccaggcatta tccgttttag tattagctaa tgaattgaaa tcttgattgt atgcagtttc
     1081 aaattgagta atacttacct gtccccaccc cacctccgcc atcgtgctca gcaccgctat
     1141 cattattaaa acatagccac cttttttcat actttcctcc ttaatttgta attttaaaaa
     1201 atatttacta tagaatgaat atcttccgag aattattcct aaaaaccgct ctctaatcgt
     1261 tcgtatcaga tattcacagt tacatccaac ggccaaaata tgagaaagat tttaagaaaa
     1321 agcaatgatt ttttagacta actatccaat ttttaactaa atttttataa gtattttaaa
     1381 agccggttcg taaagttatt tatggcataa ttgcttgccg gaattgcaat taagtgatgg
     1441 tgataatctg agcaaacccg aagtgagatt tacttaatta ataattacct aaaaaaaata
     1501 ttgacttaat aaaaactatt ttttaaaatt cacaatgaat tttataatat gggcgaaaaa
     1561 taaactaaca aaatgaaaaa agcacccaaa ctcctttttg tagtgaacac acgaaaacgg
     1621 gtgcattcga tttcgctcag ttggggaaag ttggttgccg ttctcgccgg tggagttttt
     1681 ctccttattt ttgtcattat tttaggcgcc aatttgtttt ctaatctggt ttatcaatcc
     1741 aggttaactc gtcttaagaa aaacaataat cggttggttt caacctttta cgacttgaat
     1801 aatcgcatcc agaaaatgga atcggatatt agcatgctgg ttgaaaaaga caaggctctc
     1861 cgaacctacg gtgatctccc cgtaattgat caggacatcc gccggctggg tgttggtggt
     1921 cgtttaatgc ccaaatcgga cgaattagat cagctcctgc ctgagagtga tataaaagtt
     1981 tcggaattag tcaataatct ggatcgcctg gcgcgggaaa ttaaatttga gcgcatcagt
     2041 tatgaaacga tttatgatgc tttcaaaaat cgttccgatc agattcagtc cactccgtcc
     2101 attcgaccgg tttataccgg ttttatcaat gacggttttg gttaccggag agatccgttt
     2161 accggtcgga gagagttcca ttatggttta gatattacga cgccaactgg gactccagtt
     2221 tttgttacgg ctgatggagt cgtcggtaaa tccgggtatc aaggtggata tggttacgtg
     2281 gtcgaagtcg atcacggcta cggatattca actctctatg ctcatctttc gaaaatatta
     2341 gttcgatctg gtgaagccgt taaacgcggc caaaaaattg gcgaggtcgg cactaccggc
     2401 cgcacgaccg gaccgcattt gcactacgaa gtaaagcagt ttaatatcaa taaaaatccg
     2461 cttgactttt tcttctccgg atatattcgt taagcgtaga ttcattccct catataaatt
     2521 tcttagtttt catttccaaa accggtttta tattttaaat ttcagcagtt acgatggaag
     2581 cccgaaaatt ttatatcgaa acctatggct gtcaaatgaa tgaagctgat tccgagttgg
     2641 ttattggctt actgcgcgga gctaattatc agctgacggc tattcccgaa gaggctgatt
     2701 taattctcat taattcttgt agtgtccgag aaaatgcgga aaagcgggcg attgccagat
     2761 tagcgcaatt taaaagtcta aagaaaaaga aaccggagct gttgctcgga ctgatcggat
     2821 gcgtcgctca acgaaattat gacgaaatcc tgactgaaaa caaatttgtg gatttagttt
     2881 taggcccaga ttcctatcgg aatttaccgg aatatttgaa tcaggaacag atgcctatcg
     2941 tggatgtgaa tctatcgcgc acggaagtat atgatgagct aattccttct cgcaatagcg
     3001 cggtcaatgc ctgggtctcg attatgcgcg gctgtaacaa attttgcact ttttgtatcg
     3061 tgccgtatgt acgcgggagg gaacgcagtc gttcaccgca gagcattatt gatgaaattc
     3121 gacaagcatt aaatgatggt taccgcgaag taaccttgct gggacaaaat gtcaattcct
     3181 accattatga agcatatcgc tttccggaat tgcttgaggt agttgccgct gtaccggatt
     3241 tgcgtcggat tcgttttacc tcgccccatc ccggcgacgt tgatgaaaaa atgttagcag
     3301 ttatgcaatc tcattctaat atttgtaaac agattcattt gcctcttcaa gccggctcat
     3361 cccggattct gaagttaatg aaccgttcct atgatcaagt gcattatctg aaagtagttg
     3421 acttaattag agaatttctc cctcaggttg cgcttacgac cgatattatt gttggttttc
     3481 cgggagaaac cgcagatgat tttgaacaga ctttgcaggt aatgcagaca atcaagttcg
     3541 actcagcctt tatgttcaaa tattctccgc gcccgggaac taaagcggct aaaatgaatg
     3601 acgatgtccc ggaggaggaa aaatctcgtc gtctgaaagc cgttattcaa cttcagaaaa
     3661 gtcatacatt ggaacgtaat aaagccttag tgggtacggt tcaagaggta ttaattgacg
     3721 gaaccagtaa gaaaaatccg gacgaaatga tcggccgtac cgaatctaat aaaatcgtcg
     3781 tgataaaaga aggacatcca aaaatcggtg agttttgtaa tgtccaaatc gaaaaggcta
     3841 tgggagtgtc tttatttggt aaaatcattt aaattttgag gtgattatga aatttttacg
     3901 ctgggttgta acgcttttga tcatgttttt aatcgtgttt tttgttattc agaactcaaa
     3961 tttgaatcca ccggtgaaaa tccagttttt ctggaaagca tctccagttg agatggatat
     4021 tctaattctg gtatttttga cttttatact gggacttgta attggctttt taattgctgg
     4081 tattaaaatt cttaccacta agaatgaact acgcgcttta tcgaacgatt ataaaaagtt
     4141 gaagaaggaa gttgatttac tgcgaaatca gggaattgac gaaacagaag ccgataattc
     4201 aaaaaccgaa taaacaggaa ttaatatgtc tttattggat atacttttaa cgctagttct
     4261 cattgcgctt attggattta tcgtaattaa tctgactggt cggcgtcgtc taagccggtc
     4321 caaagattcc gccagccttt atgctatggc gctcaatcat atgttacacg gcgaggatga
     4381 tgtcgcaatc cgcttgtttc gggatgtcat ccgggcagac accgataata ttgacgctta
     4441 tatcaatttg ggtattctga tgcgcaagaa tggtaagcct aaaaacgcta taaaaattca
     4501 tgaatcgctt ctctatcggc aggaaattag taatatccaa cgtctggaaa tactgcgtaa
     4561 tttagtggaa gattacatcg ccgctggtga taaaattcgc gcgctaaatt atgtcgagaa
     4621 catcctgagt attgataaga aaaatatctg ggctttagaa aagaatcata ttttgaaccg
     4681 ggacttagga cgctgggaga aagcatctga atatctggcg atggctttgg aattaaaaaa
     4741 agaacgtaac gatcggcttc tggctcttta taaagttcaa gaaggtttag tcaaatacca
     4801 ggcgggtgaa taccatgaag cccgtttaat ttttcggaaa gcgctaaaaa ttgatccaaa
     4861 ttgtgaggcg gcttattatt atattggtaa atcttatgtt ctggatcatc gggaagaaga
     4921 tgccgtcgaa gagtgggtta aatttgccga cgtagcgccg cacaaagcct atttgatttt
     4981 taaacctctg cagacgattt tatttaatct cgggaatttt ggcaatattg aaagttttta
     5041 tgaaaatctc ctacgaagaa ttcctgatga tcccaaaacc ttgattgctc tcgctgattt
     5101 ttacgaaaag aagggaaatg taaaaaaagc tatcgctatc ctggaagatt tagttgaaaa
     5161 aaatcctggc ttgtccattg ccaaaattaa tctggctaaa ctgcttgttg gacaggctga
     5221 attaaatcga gctacagcca tcctaaatga cataattgaa aaaatggttg atcagaaaga
     5281 gtatatctgc tctaattgcg gtaatcggag ttctgatatt ctgtggattt gtccggtttg
     5341 cggtttggct gatacctatt ttacggacgt atgaaacgat tcgtttttct catattgatt
     5401 ccggctttta tatttgccca aaatatcggg gaattgaagc agaaaatacg ggcgggcgaa
     5461 gttgattccg tcagaatact cttgcctgca ttaatagctg aatatcccga taatcccgag
     5521 ttactttata ttagtggaat gattgaaacc gatggtgaag cggctttgct aatttatcgt
     5581 gatgtgctca gtcgtttccc cagcagctcg gttgctgacg atgctttact gaaaattatc
     5641 gaatatttat ttactaaagg cttgtatact aaaacggaaa aatacgccag tgaattgatc
     5701 aaaacctatc cacaatcgga tttgattgac aaagcaattt atcttcgatt atgcagtttt
     5761 aacgccctta ataaacggga ttcagttgat tattattata agtattattc gactcagtac
     5821 cctaacctaa attttcattt tgaggattac aaaatcgctt ctaaggttac tagtatcgaa
     5881 tctaagaaac aatctcaacc gaccagcagt caaatatctt ctaacgctaa atcggcggag
     5941 actcaaacct attctctgcc aaaagtagaa gtgccaggac aaggcaactt tactttacaa
     6001 atgggtgttt ttggtgatcc agcaaacgct agggcgcttc gaagtaaatt agagaaactg
     6061 gggtattcgg ttactttgcg ccaaatagaa agatccggcc gcaagttgac ggcggttctg
     6121 gctggttcat tcaagtcgga agaggaggcg cgcgcggttg gaaataaatt gaaaaaagat
     6181 cataacttag attttattgt tgtcaaacaa tgaatatggg aatgaatgct accccggtgg
     6241 gggccgcggt cttcaaaacc gtcgttcccg atctaatcgg gaagctgggt tcgactccca
     6301 ggcatttccg caaagtcaaa aattagttgc gtctgatttt tcgagttatt aatttccagt
     6361 gatttttaac aagtgatggt ccgggggtgc tgacccgttt gacggggcgg ctgagaattt
     6421 accctttgaa cctgatctgg ataataccag cgaagggagt aattatgcat caaaaaaacc
     6481 tacctgtatt tttcttcatt cttttcattc ttattttaac cgaggcaaac gctttcggtg
     6541 aagaacattt aatttttgga aaattagtat ccgaaacaac cggtcagcca gtcgaatcgg
     6601 ctaatatttt gctggaagat ggctccggta cattttcgga caaggacggc aattttcgca
     6661 tcaaaacatc gtttcttccg ataaaagtga ctgttactca cattgctttc cgggatacat
     6721 ctttttcagc tacttccgaa gattgcggta cgatttactt aaagccgact gtaattaccg
     6781 gcgagtatgt tttcgttacg gccacgcggg cagtcaaagg caagacaccg gtggcttttt
     6841 cggaattatc cattgaggaa atcaaaagtc gctacaccgt agaagatgta ccgatgattt
     6901 tggctataga acccggcgtt tattcctata gtgagagcgg caatggtacc ggctattcct
     6961 acgtccagat tcgtggcttt gaccagagtc ggattgccgt gatgttaaat aacgttcctc
     7021 tgaatgataa tgaaagtcat caggtttatt gggttgatca cggtgatatt ctttctgacg
     7081 cggctgatgt ccagattcaa cgcggtatcg gcaatagctt atacggatcg tcggctttcg
     7141 gtggttcgat caatgtcttg accacgattg ggaccgaccg accgacgttt tcaattgatt
     7201 taggcggcgg ttcgtataat acttcgaaaa tacgtctggg tgcttcctcc gggcagttac
     7261 acaatggaaa gatacgcctt tcagcccgtc tctcgcagat agaatcagac ggctatcgtg
     7321 aataccatga aacctttcag cgttccggtt ctgccggtat agaatataac ggacaaaata
     7381 cttctcatca atttcgggcg ctggttggtt accaaaaaag taacttgatg tgggatggcg
     7441 ttccagccga attgatcaat gatcggatcg gacgtcgttc cggttacaaa tcgtatatag
     7501 ataattttct tcagcaggtt tattccttga atagcacctg gcggattagc gagaatctta
     7561 acttcaataa tgttgcttat ctggtgaaag gtgacggtta ttacgagtca gaaaaatcag
     7621 ccgcctataa tctggctgtt gatgatgcaa ctgcccgaga tgaatttcgg gattttttgc
     7681 gttctttcta ccttgatcaa tattacccga ctgatgctga cagtcagtca ctggaattaa
     7741 cccgccgtaa atggattgtg aattcgtatt acggaatcgt accgactttg acatttatca
     7801 aagctaagta tcgcctcgat atcggcgggg aatttaaata ctataacggc aatcattttg
     7861 gtgaaatttc ccgattttcc gatcagaatt tgatcgcgca aattgggaaa ggctggtatc
     7921 gctattatcg ttatatcggt gaaaagtcaa ttgcaaccgg ctttgttcac tttacgcttg
     7981 aaccgctaaa gaaattgaaa ttgatcactg atttgcaata tcaacacttg caatggaatc
     8041 tggatcagaa aaaaatcggt tatgcgattg ggtatgatat ttccgctaag tgggatttct
     8101 taaatcctcg atttggagct atatatgaac tatcaaatga cttttcaatt tttgctaatt
     8161 acggtcgctc gcaaaaagaa ccagccgatg aacaaattat tgaagccgac gattattgga
     8221 gcgaacctca aaaagccgcc gcggagttga ttgatgatta cgaactgggc gccaactatc
     8281 gaggtcgaaa gctggatgtt gacctgaatc tttatcggat aaattataaa aatgaacagt
     8341 taaaaaatat tgatatcgag caggaaggtg aatatgtgta tacacaggcg tcggcgacca
     8401 ttcatgaagg attagaatgg gaagttcgct atcaattcaa cccactctgg gcattgactc
     8461 taaacggcac tttcagccgc catgtttttg aaagtggttc ctataaaggt aatactctac
     8521 ccaatatccc gggtatactt tttaatggga gtttggatgt caagccggtg aaaaatttgt
     8581 cgatttcggc gattatccgt tatgttggcg aacaatatat tgacgaagcc aatatcggaa
     8641 tgaatccggc ttattggcta actgatgtcg gactgagata ttcattcggt cgggttgaaa
     8701 tcggcgcgaa agtcaataac ttattcaacc ggctttactc gacttatggc tatggctatg
     8761 aatggggtga atactatgct tattattggc ctggtgctac ccgcaatgca tttttttcga
     8821 tttcgtacca tttagattga taaaaataat tgtaggacag gcattcttgc ctgtctatgt
     8881 taaggacagg taatgtttat tagacagcct ggaaaggctg ttctacgaat tatttattgt
     8941 atcaatccga ccaaaaataa actcaaatcc ggaatataag taatcagaac taaagcaatt
     9001 agcataataa atagaaacgg cagagaaatc cgataaagtt ccgttatgga tttatcaaac
     9061 cgatagctgg aaatgaacaa attcaatcca attggcggag tcaagtaacc aatttcaaga
     9121 ttagtgagaa aaataattcc gagatgtacc gggttaatgc cataactgag agcaatcgga
     9181 acaatcagcg gcacgacaat aataatggcg gagaaaatat cgagcatcat cccagtgatt
     9241 aatagaaaaa tattcaatac taacaaaaag gttattttag aagaaaaaac ctgctgagcc
     9301 cagagcagag ctttctgggg aatctcggcg tcaattagat aagccgttgc tcccatcgaa
     9361 caacttaaaa taatcaaaat cccgcctacc aattgcacac tatcgcgcat aatgcggatc
     9421 aaatcccgcc ggaaggataa atcgcggttt aggatgaatt ccgctatcaa tacataaaaa
     9481 gcagttacag ccgaagcttc caccgccgtt ataaatccgc cgtaaattcc acccaagata
     9541 atgaccggta tcggcaattc ccagaccgtt tctttgaggg ctcgccctaa attctgccag
     9601 gaaaaaggta ttcgttcgct cttttttaaa acatcattac ggacagcata aatagagagc
     9661 aggataatca atagaattcc aggaataagc cccgcaataa aaagctgatc gatcgaaacc
     9721 gaagctacaa caccatataa aatcaaaggc agactgggtg gaaataataa tccgagcgag
     9781 ccggaactgg taatcagtcc cagagcaaat ttctctgaat agttattttt aagcaaaatt
     9841 ggtagaatca aaccgcctaa ggcgatgata gtaatcccgg acgcaccggt gaagcaggta
     9901 aaaagagcgc aagttacaag tgagccaatt gcaacgccgc cgggtatcca acctaacaaa
     9961 gcctccgcta agttcaaaag ccttcgcggt gcactgcttt cagctaaaac gtagccagct
    10021 aacgtgaata agggaattgc caggagagtc ggcgccgaag ctacccgata catttcgacg
    10081 atgacaaccg ttggatcaat tcccgaaatg gcaaattgaa taatgccggt tatgccaatt
    10141 gccgcgaaga gcggcaatcc gcacaaaatc aaaacaacta tcagaactaa aaacaacaga
    10201 gccattaatt ttcgagattt tgggatttat ccattaaacg cagaagaaaa gtcaatgcca
    10261 gcaggaaaaa tccaaccggc agaataattt cgataatcca ggctggaata actccggcaa
    10321 tcatcgaatt catacttatt tctaaacgca agaattgcca ggcgtaccag ctgagacaca
    10381 ggcaaatcgc cgccgctgtt aaattgagaa tccagtttaa tactaagcga gttttgcctt
    10441 ttacaatacg cggcagaatg tcaatcgaaa tgtgtttact acgtaaggtc gttaatgccg
    10501 ccccacagaa ggcgatccac aaaactccgt tgcgcatcaa ctcatccaca acatttaacc
    10561 caatcgcaaa aaaattgcgc aaaattacct gcaggaaagc cattacaact aaaaatagca
    10621 tcaagacaac cgctaaggcg gtgacaaatt tttcgattat agttctaatt ttattcagca
    10681 tgctcagcgc gatattcatc taaaagcgcc agaacccggt cgagcatttc acgagaatac
    10741 aaaacaccag ccaatcggtc tctcgttcgt actccggcct cgatgaattc ggtggccgcg
    10801 tcgcttttca ggtttgtgag ttgcatacca ttttctttaa gaacctggat agattccaaa
    10861 ttctcctgcc taccagccag cacgatctgg cgtgtatttt ccttggtttt tttgattaga
    10921 atatttcgta aatcttccgg aatagtgttc atttttttag tcgtaatcag gattgcgcca
    10981 atagaattag ttaggggata attcagcata tatttagttt tggtatgcca ttgcattgcc
    11041 aacgcgccgt aaggagatat ataaactccg tcgattaacc cggtttgtaa gccagtatga
    11101 acatcggtga tatcgagtgg aatcgccgct attttcattt cgtcaaaagt ggctttggca
    11161 agcggatcgc ctttccagag ccacattttg acatttttaa aatccgctaa tctggtgact
    11221 ggcgtgttgg tgaaaagata aacaaaacct acctcagccc aacctgccaa aaaaaatccc
    11281 ttctcgagaa atttagtttg aaattcgtcg aaaagagcgt cacaaacgta atcaacctcg
    11341 gcgctattcc gaaaaagaaa cggcaaatcg aaaacgcgca cttctggtac aatttcaccc
    11401 aaaccgactc cggtgaaagt cccgacatcc aattgcccta attgcatttt acgtaaaatg
    11461 tctttttccc cgccctgtac gccactagcg taaacccgaa atttgacctg accgttagtt
    11521 agagcatcca cttccttctc aaactgtcgg aattgtcgca tccagctaga accttctgga
    11581 gcgacggtcg caacttttat cgtatggact ttttgagcca cgaggttagg aattgtacat
    11641 aaaattataa gaatcaccgc attgatatat ttcattcgca tttttaatct acctttcgtt
    11701 ggtttctaat atttcattta ggtcgagaaa caactcggca ttctggcgca gattggtggc
    11761 tttttgtttg gcaagcgcat taaagagtcg aatttctgga gcactttcga ggtcgaaagt
    11821 ctcgatctcg gttaaaaggt tatcaaatag ttctttatcc tgaacctgta cagcgtaata
    11881 gcgagcgtaa tacaatttcg tcattaaaaa attcggattg cgttcgatat ttttcagata
    11941 ataactgcgc cccaattccg gattgccgcc cagcattggt gggcgagcgg aataaaatgt
    12001 tccgtaaaat atatccacac cgccgaaatt atagccagcg tccaactcgg ctactcgacg
    12061 catcaagagt tcaaccttag ctaagtcccc caataagcgc ggctgatcga gattttgcag
    12121 aatatacatt ccccaattat atccgaccca gaacaattgc ggcacgtcgc ttttacggct
    12181 ttctaccagg agcttttcca ggtcggcagg cgtgatattg cgcgagaatc ttatttgcgg
    12241 tggtagtgat ttgaaagcat attctaagcc gcgaacataa agcagaccgg cgcgctgagg
    12301 gtctttgtct tcgacaaaag ccattgcata ggctccaaaa gcctgagccg ccatcaggtt
    12361 taaacgcgga ttctctggat cctgcgcaac taaaatgacg attgtctcca aattactcga
    12421 aaggaatctt tcagcgagtt ctaaatcgcc ttcttgatat attatttcga taccgctgtc
    12481 gaaaacgttg gaaactttat tgaatagaaa gcgctgagga ctgcatgaaa gcccgcctag
    12541 acataaaatc agggtaaaac ttagaaattt gatagctggt attgaatttt tactcgacat
    12601 tataacggta aaaatttata ttctttgttt tgaaaaacaa attttttaga atattttaaa
    12661 tatggtatta agtatcacga tgaaacaaat ggttaaaact attcttttac gtagtatctg
    12721 gctcggtctg ggtgttattg taatcggatt aattttcaat tggttatcac cgaatggtat
    12781 tccgcttgtg gctaaatatc agacggttac agtccgaggt aagagtcaaa aaattccgct
    12841 ttttatgaat cgcaaggctc agaccaatca ggaaactgtt ttcgaaatca gcccggtttc
    12901 cgagcttgat accgaaaaag cctggcaatt ttttactaat ggtggtacca tctttatcga
    12961 tactcgagaa tatgaggact atctcgccgg gcatattgcc ggagcaatat cgattccatt
    13021 gtcggaattt caaaacgatc caaccattgt tgaggaatta gaccggcagg tgcggatcgt
    13081 gacctattgt gacggcagtg attgtcagtc aagtattgat atggcagtac gcttaaatga
    13141 aatgggtttc gagcaggttt ggttttattt cggtggctgg aaagagtggg tagaggctga
    13201 tttaccgatt gtaaaaggga gcaagccatg aagtccaaat tctgggattg gttggaatta
    13261 tttatccgat tggttattgg tttcttgttc atttatgccg ctgtcggcaa gatcatccac
    13321 cccgaaaagt ttgccgtgat tgtgtataac tatcgcgttc tacctgttga attggttaac
    13381 atcacggcta tcctggtacc atggctggag attgcgcttg gtgtaacctt aatattggga
    13441 atctggctgg aaacagcggc ttttctccta agtattttga tcattgtctt tattggcttg
    13501 attatcagcg ctattatacg gggtctgaat atcgaatgcg gttgctttac tctatcgaaa
    13561 accggttcgc tggtcagctg gaagcgagtg gcggaagacc tcctgatttt ggcgggcagt
    13621 ctgttcattt tcttccggca tctaccgcaa gaacaatcta cgaccgaata gtcttaccgg
    13681 tcggattata gtttatgtct ttttggtaaa atgtttttaa tccagattga tttagttcaa
    13741 tcctgccaat aattttaaca acttatttta taaggaataa gatacgctga agaagttact
    13801 tttttgcgat ggataacggc agtagccaat gataaattta tctaagttgt atgccacgcc
    13861 ccaggttatg taattttcat tgttttcaat atgttgaccg cagtggatgt aaagtcgctt
    13921 gtagaagctg aaagcgccgc cgattaagtg actttttaaa tgatttgaag agttagaatc
    13981 ataataatct tcaatgaaat cgtaggaaat cgtagcgctg atcaagtcct tattaagcag
    14041 tcgccaacca ccgccgagtc gtaagcgagg cacgagtttc gacttataat tggtaaaatc
    14101 attgcggtat ttaatagccg gtccgagatt gcaaaatgcg gcgcccaggg ataatccacc
    14161 tgttttattt tctccaatag ttaggaatgg caggagattg ttgatctgaa tacccaggtc
    14221 gcaggctaaa cccttgccgt taatagattg aatgccatcg ctcgcagtgt tcggggcgtt
    14281 agtatatatg aattttgttg ccgctcctag agtaattttt ctggtaaaat tgcatccata
    14341 tccgatggaa acatagataa agttatagga aaaatcggtt gcagtgtaat cgtctccggt
    14401 tattttcgca tcgagattgt acaggtataa actggttccc aaaccgccac ggtgtttaaa
    14461 atttctaaca gctccggcat aatatatttc actgttgccg agccaggtac cataattggc
    14521 tgaggcactt aattcatcgc cattggctaa accagctgga ttatagtaaa gtgccgcgac
    14581 atcgttgacg atcgccgtgg cggctccggc taacgatgcg taacgcgtct cgggattgat
    14641 taatagccag acttcatcag cgaaaacaaa agatgcgaga gaaaataaga gcaaaatatt
    14701 ttgaaggatt ctatattttg aaatattttg catgaaaatt ctaaatatcc atttggttgt
    14761 gtattttatt caaatgctgg tcaaaaatca atattttaaa attcttgtat tttattaatt
    14821 ttcattgaga aagttattat taactgtact ccgtcaaccc taaccattaa cttttccttg
    14881 aaaaaatggg ctctgaaata catcaatgag ccaaaactct tgattaagta taatttttta
    14941 gagaacttta tgctgaattt aaaataagtt tgattgaaaa taaatgatgt tagaaaatat
    15001 atgactgaaa atacccgcgt tcgttttgct cctagtccaa ctggttcatt acatctggga
    15061 ggtgcgcgaa cagctattta caactggcta tttgctcgct cggtcggtgg caaatttctc
    15121 ttgcgcattg aagataccga cgtcgagcgt tcacgcaaag aattggtgaa tcaaatttgc
    15181 gattcgttac aatggctggg attgaattgg gatgaagaac cagtatccca gtcgcagaga
    15241 ctcgaacttt accaacagaa agttactgaa ttgttgaaag ccggcaaggc ttatcgctgt
    15301 ttctgcagta gtgaggaact ggaagcggaa aaagccgcta caattaaaca gaataaagct
    15361 tatcgttata gtggtaaatg ccgtagtcta tcgctccaac agtcagaaga actgatggca
    15421 cagaacaaac cttacactgt ccgtatcaaa ctcgaaccgg gagtcattga atgggatgat
    15481 ctggtttacg gacatattgc ggtgaataat aatgaactcg atgattttat cattcaacgt
    15541 tcgaatggag ttccagtcta tcagatggcg gttgtcgttg atgacattga tatgcgaata
    15601 actcatataa ttcggggtga agaccatatt ccgaatacgc cgaaacagat taatctgttt
    15661 cgtgcgtttg gtcagccgat tccgcaattt gctcacttgc cgcttctgct tggtccagat
    15721 aataagcgcc tcagtaaaag gcatggcgca accggcgtgg acgaatatcg cgaacaggga
    15781 tttcccgccg aagccgtcgt gaattattta gctttactgg gctgggcacc gcagaatgat
    15841 caggaagtgc tgagtattga cgaaatcatc caacaatttc aattgaccgc aatttcccgc
    15901 aaaccggctg tttttgatat aaagaaattg gaatggatta gcggtgaaca tcttaaccgt
    15961 atgcccgtcg cgaaaattag atcggtttta gttcccaaat taattgcggc cgggcttatc
    16021 aatcagcaag aatctgaaaa ccggactcag tacgttgatt ccgttatcga gttattacgc
    16081 ggtcgcgtca gaacttataa tcagtacctc gaatggggct gctatttttt ccaagaccca
    16141 gaaaattatg atgaaaaagc tgccgctaaa tactggcaca caccggagac caatgaacat
    16201 ctggctaaat taagtcaaga gttggcaacg ctgacagatt tttcggctaa tactatcgaa
    16261 gaaacattga gacgaaccgc agaacatgcc ggattaaaag ccgctgattt gattcatcct
    16321 ctgcgtctgg ctttaaccgg tttcggtatt agtcccggta tttttcaagt agcagagatt
    16381 ctggggaaag aaaccgttct gcgccgaata caaaaagcca ttgaatattt caaaacctga
    16441 ggaattaaaa tgaaaaatgg ttggcaaatc gcatattttc taatgttatc atttttatta
    16501 tttggattgg tcggtgcgca gacgcccaat tctcatccga ttaatctttt cattctcacc
    16561 ttcactaatt atgagggcgc gccagaaatt gattggtgga aagaaggctt tgttgatttc
    16621 ttgatggact atttcagtaa taatccagat cttaatgtcc atcgcatcaa tgacccggca
    16681 accataccaa accaaattaa gaatctctca gaaacggaaa ttaccagatg ctacctttta
    16741 accggcaatt ataaaataat tgatcaaaaa ttcgaggttg atttggaatt aattgacatt
    16801 aatacctggc gacttctcca gaaaaagaac atctcgattc aatcagcaga tatggcaaag
    16861 attatcgaat ctgtcaatca cggcgcgact gaagtactta ctccatattt aataaaaaaa
    16921 gccgagacca ccgtcgctca ggaaattagc tcaccatctc aaccgacgaa aaataattac
    16981 cagaaggctg ttgccgcaag tgtagcaatc tcatcagtga ttgataagtt ttcggaaact
    17041 catcctgagt cagcgcaaca cgaaccaagt tttggtggca ccgtgactaa tgaactttcg
    17101 cttaaattcg gagaggcgat tgcccaaact ggtagttttc aatatactat caatcagatc
    17161 atccgcaatc cctataaact tgaaatcagc gatccacttt tccggcgcct ttctgcaaac
    17221 gatgagataa ttaacgttac tttttttatt aattatgttc ttagtcgtga tctaattcaa
    17281 gatatgttga caacttttcc ttatgaaatt cagacaaaaa atgatgaata taccgaatat
    17341 attttcagtg gtaagcggtt tgcctatagc gaagatttta ttaaggcgat tgcgcgtggc
    17401 gattatcgga ctcacccggt atatgcattt ataactaaca ccgaaaccgt agcttgcgtt
    17461 ctgattgata tgccaataat gtaccgcaaa aatcttattt cttcttcaaa agtacaattt
    17521 acagccaaat atcagccttt aatcaatgtc acggctaacg gttggaatgt cgtcatccaa
    17581 cttaaaaata ccgatgcgag agccgaatac aactttgata taagcttcga acaattgaaa
    17641 aacctcagcc gtatagatgt gatgtttctt tcagaagccg acattcaaga atatttaaaa
    17701 ataatgaatt aagatttatt tgattatcta tttgttcttg aatatattat aattaagaaa
    17761 taaatatatg aaactgatca cacaaattaa tccgtgtctc agaattaaga ggaaaaatgt
    17821 ttagctatca ttaaagattt taaatttaga ggggaaataa tgaatcagta taaaatatta
    17881 attgtggatg atgaacaaaa tgtatgtgaa tttctcgggg agtttttgca ggataaaggc
    17941 tatttagtta tcaaagccag atcaggtagc aaggcgctcc gttacctgga aaaaaataat
    18001 ccggatctgg ttttattaga tattctgatg cccggaatga gcggccttga agtacttaaa
    18061 cgtattcgaa aattatatcc cgatttacca gttattatct taactggggt gaaggataaa
    18121 caggtagttg acgatatcat caatgtagga cccgttgatt ttattcccaa accgattgat
    18181 ctggatgttc tcgaaaaatg tatttcaagc aatctcgcaa aaaaataagg taataataat
    18241 ggatactgcc attttatttt tacctgaacg aatggcatct aacaaagcca acagtatctt
    18301 taatactatc cgaggcatat tacatactca actgaacttg aatatcgtta ttcaaaacct
    18361 ccaaaacagc cagcctattt atgaacaaat aaccagcatc aaaaatgccc tcgtatttgc
    18421 ttctatttat gattctattt atttagagaa atttgaaaat tttcagccgc tctttaaaat
    18481 taaaacaaaa tcatctgata atattctggt tattccccag aaagcgactg ttcgccatct
    18541 taaagaactg gcaggtggta aggtgctgat tactaaggga atagatcata actgcgtcaa
    18601 tccggtgatt ctatacttga ttaaaaattg cggaatattt tttgctccaa aatcattatc
    18661 aatatactat gagccaaaaa cattattaga tcaattgcta aaaggtgacg tttcggcgat
    18721 tcttaccaat acttttcaat ttgatttatg ggaagccgat gaacaacaaa gtttaaaaat
    18781 cctcggtaaa tgtccggaag aatctgaaat tgtcgcctta atcgggccta aatttaatgt
    18841 aagaaagtta aagaagaatt ttaatgattg gttgcaggaa cacggttcag attatttcaa
    18901 agatggttat aaattacaat caattgataa atatgaaaat atactgctaa ttgaggctat
    18961 cgaaggatta ggatacaaat taaaagattt cattgaaaat taccctgatt tgattatcaa
    19021 aaatattaaa agtaaattag cggaagaggt tgaaactctg aatgaaaaat atagcgggtt
    19081 acaagttttt aatgaaaaat tagttgatat gtataaagag attcgcgaga gccatgatcg
    19141 tctttatcaa gaaatagatt ctgccgtcca aaatcagatt ttatttctca aagatggcac
    19201 aataatcggc atctcgcggg gctttatgaa ttgggtacaa aaatcccggc cggaaatcat
    19261 cggtaaaaat attaacgatt ttattaatcc aaatataaat aagcctttta atgaattgat
    19321 tcagcaggtt gattatggtt tgatcaaatc atttggaata cgtttaaaaa atacaaacga
    19381 caatgacact acagctaaaa tggattttac catacttgaa ttgcgggact ctaaagtcgt
    19441 aatgggaatt gtaagtatga taacagatag ataaaaattt aaatgggagt agaaaaaatg
    19501 aagaagtata tcgtttcgtt agcattttta ctgttgtgca atcttgcgat cgctcaaaat
    19561 cagatggcga ctgttctgaa agtagccgga gacgtcaaat tacggactaa agggcgtagt
    19621 gatttttcga gaaaggtgac catgggtatg ggtattaaca ccggagatgc tctaaagact
    19681 ggttccgacg gatttgtagc tattatcttc accgaggata aaagtctgct taaaattcgc
    19741 aatgattccg aaattgaact cattaaggat aatttaggcg atcgaacctc aaaagttact
    19801 caaggtaaag ttctggctga aattactccg ggcattaaga catccttccg gatagagact
    19861 ccgacttcgg tggcgtcggt taagggaacc aaattctgga caattaccat tcccgatttc
    19921 ggagataaat tttatggcgt cgaagggcaa gtaggtattt taaatttaat tacgggtttg
    19981 gaggctgact tggaaccggg aaaaatggta gtatcgacta ttgatggaga actattcagt
    20041 ttaccggtag atccagatga gattcctgtt gatcctgagg agatgaaacc cgaagaagag
    20101 ccgggtgaac catcgggaat gaagatggaa gaagaaactg aaatgcctgc aatgggtgcc
    20161 atcgaggcga ctggtcaacc accggccgaa tcagtagccg aagcaggtaa aaaagaggag
    20221 gaagcccaaa aagcaaaacc ctttgggatg ggattgggac ttggctcagt tacgattggc
    20281 gggaaaattt ataatcagat tgccctgcgc cccgaattta aatttgggaa attaggtgtt
    20341 tctcttgata ttgcctttta tatggacgaa aatggcaaca tccgcaaaga agaatgggat
    20401 gaatttatcg attatattga caaaatatat tatgtgcgtt gggctcagca gggggatccg
    20461 ttcttcatca aagttggggc gttggataat gttagtatgg ggtatgggat cctattaaac
    20521 ggctattcca atacaaccga atatccgcaa gtccgcaaag tcggtatcca taccggaatg
    20581 caatggagca aaattggctg ggaagcctac atagctaatt taaaagagat taccggaccg
    20641 ggattaatgg ctgggaggtt gacttataga ccgattagca agtttccatt ggttttggga
    20701 actactgttg ttgctgatgt ttatccttac aaaggtttac cggatgcgga tgaagatgat
    20761 gtcgccgacg cgctggattt atatccggag gctgatgata atgttgtcct cgattcactt
    20821 aaaacaggtt ttactttagg tcaacgcgaa tttcttagaa ataacggctt taatattcca
    20881 agtgaaacca tcatacagaa tgggataact aagctctccg attatgatcg gcttttaaat
    20941 ggagcggttt cggttgatgt aggagtgcct attctgaata agaaatcctt caatttgacg
    21001 gtttatggcc aagccgcctc ctttttcccc gttgaggatt cagccgtagt ttataacagt
    21061 gcaactcaga gctatgaccg tgtagctttt actccgggat atggatttgc agtcccaggt
    21121 gtgaaaatgg gaatcgccag aatcgcaaac ctgactctgg aatatcgcta tgccgctgaa
    21181 aattttctat tcaattactg ggaccgagct tatgattatg agcgagtaca aattcgtaat
    21241 aatcaaatat acaccaaaca acaaatgtcg ctgatgcaga gttcgatgca gggagtttac
    21301 ggagctgttg acgtcaatat catgaattat ttcattcttg gtggttatta tcaacacatg
    21361 ttctcggaag ggaccgagat caagagtttt atggcgtcgg catctatacc gaagggaata
    21421 gttccaaaac tggctgatgc gacagctttt tatcaacgta ataatgatga aaatcctttc
    21481 gattttaaga acccttctga aaatacgata ctcggttaca ggataggttt tgaattgggt
    21541 ggcggagcga ttatctacta taaattccag agaacttacc gtgattttga cggtaatgga
    21601 atagttgacc ccgatctgga agcagttaat ctaactacca tcgaaactgg gtttagtttt
    21661 taannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggatatc aagagcagtt
    21781 gttatatttt actccttatc agatgtacct aatcgcttca cgtatctctg tccgggagcc
    21841 gctcaccgac gagagccgtc aattgattca aaagaaatac aagcctttat acgccgaaaa
    21901 atttttagct gagataccga tgaattccta ctgtcgtcta aaattgaaag ataaaagtca
    21961 atttgatgcg gtttattatc gtctcaaggg tgatagcttg ttattttgga ttgatcgcca
    22021 ggttgttcca ataaatagga acgatttggt caaattaaaa tactgggata attatcgtcc
    22081 gaataactgg gtcaagtggg gtactatggg aagtgtcgcg gttatatcat attttggtag
    22141 tgggctggta gccgattggc ttaatattac cacaaaaaat gcgattttaa ctcagttcgc
    22201 ggccgcctcc gtcggttgtg ttatcggata taaaatttct ccaatagtca atgaatatat
    22261 tatgccgtcc actgtgataa aatttccgac gggcaatata aaaagacttg acactatcgc
    22321 aagaatgtgt tacaatatta gaaatattaa ggataaaata tggcaaatta ttacacctta
    22381 aacaaaaatc tttccacgcg gcgaacgact tctccaactg aagaaacgcc tccgtcgccc
    22441 gcagtcaaaa ttgactttag cgacaccgaa caacgctacg aatatattcg gagtaaatta
    22501 gatgacatta tggaaaatat tggtcagtgt tatagcgaac gcctgaccaa ggaattgctt
    22561 caccgtttgg aaaaaacaat tctcgatttc catacggagg ttattgcgct tttagataag
    22621 cttgaaaagc tggaaataga acgcaatcaa ttgccagaac ggactcctgt ttctgaaagc
    22681 gttagcaccc cggttgaatc taaatcaggt gaacgcgcaa aacggataga ggaaagtgaa
    22741 caaagaaaag ctggtatagt catagaatca gaattgaata aaaaaaccga aagtgaaccg
    22801 aagaggaggt tttttaatcg tagaaaataa gtattcaatt atcttttgat agattaactg
    22861 atttttagta atttaaaaat atctgttatt tgataataga atacgatttt aacggtggtc
    22921 aggacatagt ttctgaatcg cggggtaata acggaattat agtctatcta actgatttca
    22981 actagagcgg ggaagagatt caatcgtacc tgaaaaagtg accactaaaa attatcatac
    23041 gttgaacacg gggctgtagc tcagctggga gagcgcaggg ttcgcatctc tgaggtcgtg
    23101 ggttcgatcc ccatcagctc cactgttatt tgatacgaac aaaatgattg gctatgctac
    23161 acggggagct agcggtgccc tgtaacctga aatccgcttt agcagggtgg aatgccaggc
    23221 agaggatttg gtcggggata ataggttaaa tcggaagcga tgacgctttc agttttgcgc
    23281 aatggaaaat tatcaaaccc cgtcaggtcc ggaaggaagc agcggtagat aaccctttct
    23341 atgtgccgca aaatcagcga aagccagccg acgactttaa ccgaatatcc ctggacggat
    23401 tcaatgcctg gtgcatagcc taagtaaata tgaattataa agtaatttct cgtaaatatc
    23461 gccctcaaaa tttcaatgaa atcattggcc aagaccacat ttgtacgact ctgcaaaatg
    23521 ccctcaaaag tggccgcatt gctcatgcct atctctttac cggttcgcgc ggaattggta
    23581 aaacttcgac tgcccgaatt ctagccaagg cactcaattg ccttaatccg caagactata
    23641 atccctgtaa tgtctgccag aattgtgttg aaataaccaa tagtcgcagt ctggacgtga
    23701 ttgaaattga cggcgcttcc aatcggggaa ttgaccaggt acgcgaactg cgggaaaatg
    23761 tcaagtatcc gccatccaat tcgaaatacc gaattttcat cattgatgaa gtccatatgc
    23821 tcacgcgcga agcatttaac gcacttttga aaacactcga ggaaccaccg ccgcatgttg
    23881 tttttatctt tgctactacg gaaccattga aagtaccgcc tacgattatt tcccgctgtc
    23941 aaaggtatga ttttcatcgc attccagttc gtgaaattgt ggggcaatta aagaaaattg
    24001 cggaatccga aaatttaacg gtttcggatg acatcttgac tttgttagcc aaaaagtccg
    24061 aaggagcgat gcgcgatgcc gaaagtctcc tcgagcaggt tgtttcattc tctagtgaga
    24121 atgtgaattt gaattctatc caaaaattgc ttggtctgac tgattacgaa tattatttca
    24181 caatcagtaa ccaggttttc gagcacgatc ttaatgcttt gattaaatcg gctgaagaag
    24241 tattcaataa tggcgttaat atcagtgagt ttctgattgg cttatccgaa cattttcgga
    24301 atttgttgat tacagcgatt gttaaatccg ccgccgatct ggatctcccg gattcattga
    24361 aaaatcatta tcaagaagag gcacaacggt ggtcggttaa cgatctgatg cgtttattaa
    24421 aattagttgg tgaagcgcag gctggtctga aagcggctat gaatcaacga actcatctcg
    24481 aatttgccct tctccgaatg ggagcaatgg aaaagaccgt cacgattcaa tcaatcctgc
    24541 agaatattga tgctcgtcaa tctggaggaa taggacagat aaagccagtc gcccaaacga
    24601 tggatttttt ttcaaccaag ccggtggttc ccaaaacagt ccctcatcca gatagtaacg
    24661 ttattaaaga gaaagtatct gataattata tacctgaaaa aaaatctgaa ccaatttcaa
    24721 agtcgtttga tagtttagtc acgtttgaag cgattcaggc acaatggtcg acaattattt
    24781 tgaaattgga agaaagcaat ctttcgttgg ctgaatttct caaagtttca aagccgatgc
    24841 aattaaatgg aaatgttctg gatctaggtt ttaccagtga acaagatttt gctcataaaa
    24901 atgttgccaa aagagcaccg actatcgaac agattttgat tggttttttc cataaaccga
    24961 tcaaaattca ttgtatccaa atagaatcag ctgcggaatc ggctgatacc gtaaaaaagc
    25021 cgatcgatga aatgaccgaa aaagtcgtag atatgtttga tggcgaaata attattacat
    25081 aaggagtatt gatgatacct aaaggtggac taaataattt gatgaaacaa ttttcaaaag
    25141 ttcaagccga aatggaacga gtgcaggaag aacttgccaa tctaactgtc gaaggcagtg
    25201 ccggaggcgg aatggttaag gtcgtagcta atggtaagca ggaaattcaa tctattaaaa
    25261 ttgatccgga aatattaaaa gacgatgtag aaatggttga agatatgatc gttgcggctg
    25321 tcaaccaggc tttggctcgt gcccaggaat tatcgcaaga aaaaatggca gacctaaccg
    25381 gcggaatgtt atccaatcta ccaggaggaa tgaaaatacc cggtttatga gtgctcttcc
    25441 cgattcgtta caaaatttga ttgaacgttt tgcccgattt cccggaatag gccgcaagtc
    25501 ggcccagcgt ctggctttct atttgctgaa agccgatcgg gaggaagctg ttgcattagc
    25561 caaagccatt atagatgtca aagatcgcat ttataaatgt tcgcagtgtt ataatatttc
    25621 cgaaaccgat ccttgtaaaa tttgcactga tgaaaaacgc gaccacacga cgatctgtgt
    25681 tgtacaagag ccgatggatg tgttctcgat cgaaaagacc ggtcattatc gcggcctcta
    25741 tcatgtttta ggaggagtat tgtcgccgtt gaacgggatt gggcctgatg acctgaatat
    25801 tcaaggtttg ttataccgac ttgccaatgt gaaggaagta attcttgcca ttaaccttac
    25861 ccgcgacggt gagacgacgg cgctctatct atcaaaatta cttaaaagta aaaatgtaaa
    25921 agtttctcgc attgctcagg gacttccggc cggcatagaa ctggagtatg ccgacgaaat
    25981 taccctaacg cacgctcttg agggtaggac aagtttctaa tatgaaaaaa ctatttaact
    26041 ggcctaatct attgactttt ttccggattc tcctaacgcc tctctttctc tattttctgt
    26101 tcggtaacca accttatttt gaaatattcg ccttgttaat ttttattata gcatcggtaa
    26161 ccgacatcta cgatggctat attgctcgga aatatgcgag cgtgaccaag ctcggcaaat
    26221 ttcttgatcc tttagcggat aaaattctga tgtctgccgc ctttatttca tttgttattt
    26281 tagatctggt tcctctctgg atggttattc tggtcattct acgtgatttt atcgtaaccg
    26341 gtattagaat attaatgaat tcgcgccacc tgacaatgga aactcgcaaa tcagcgaaaa
    26401 cgaaaaccgg agtgcaagtc ggcgttgtct gtttcattct agcttatttg attactcagc
    26461 gctgggaaat atttctgggt atctccgact atgtcaggtt aattgaagaa tatagaatta
    26521 tttatatctt gatgctgata accacaatta ttacaatctg gactggggtg gaatatattt
    26581 tagtaaataa agataaaatt cagcaagctc tgaaaaaatg aatattaccg gagctaaaaa
    26641 actactctcc cggataatag cgagcgtttg cttcatcggc tatcttccat ttgcaccggg
    26701 cacttggggt acagtagcag gcttagttgt ctggttctta ttgccatggc actcattctg
    26761 gttcgactta attctagtga cagtcacttt ttttattggc atttttactt ctgagtatat
    26821 cgaaaaacaa attggcgaga tggacccagc gtatatagta attgatgaag tcgttggagt
    26881 ctggctggcg ttggcaatta ttccgacttt aacttatccc aaagatttgg cattgatcgt
    26941 tttagtgttt cttatttttc gaatatttga tattaaaaaa ttcaaaccaa ttcgcaaatt
    27001 ggagttgatc ggtggtggct ttggtataat ggcagacgat atcctggctg gtatttttac
    27061 cggtattatc gtcaatttgg gaattctgat cctgtgacca atgcggctat catttcgatc
    27121 gggaatgaac taatctccgg actcgttaat aacaccaact ctgcctatat tgctcggaca
    27181 ttaaataatt ttggtattgt ggttagctta attgtaaccg taggcgatga gcaaaaacat
    27241 atctggacag ttctcgataa tttacctccg gaaatcaatt tggtaatgat caccggcggt
    27301 ttaggtccca cgaatgatga tataacagtt tctgcccttg ctcgctattt taatctgcca
    27361 cttgtattta ataaagaaac agccagtaat ttagtgaagc attttgcaaa acggggacta
    27421 aagatgccag aaataaatct aaaacaagcc tacctaccaa aggaagctgt tattctcgat
    27481 aatccggttg gttcagctcc cggcttgaaa attgaacatc aaaatcgtgt ttattatgtt
    27541 ttgccgggtg tccctagaga agtagaagcg ataatgacca aaataatcgc tccgcaatta
    27601 gccgctctcg gccataaagc tattgtaaaa aagtacctgc acattaccgg aatacctgaa
    27661 tctcagattt atacattatt aaaagaatgg attgaacaac attctcacgt caaagtagct
    27721 ttcctaccgc agacggcgca tattgacctc gaactgacga cttctgaacc ccaaaaaagt
    27781 gttagaatag acgaatatgt caaggtgatt acagaaattc tcggcgatta cgtctttggt
    27841 tttgatgatg atactctcga atcggttatc gctcatcttt tattacagaa gcatctgacg
    27901 gtcgcggtag ccgaatcctg taccggcggt ctgattgcca gtcgtttaac tgatgttccc
    27961 ggtagttcga attattttaa aactggaatc gttgcctata gcaatgccac gaaaattaat
    28021 ttacttggag ttaaaccgga aattatcgaa aaatacggcg cggtgagttc ggaagttgcc
    28081 aatcaaatgg caacccgagt gcgacagatt tccgggtgtg atattggttt gagttccacc
    28141 gggatagccg gtccctctgg tggtagtgat gccaaaccgg ttggattggt ctggattggg
    28201 gtttccgaca aaaacgctac tcggactttt caataccaat ttagtggcga tcgctcgact
    28261 aataagttac gattttctca ggcggcgtta aatcaactgc gtttatattt aataggtaaa
    28321 taaaagatga aaagaacttt cattgccata ccggttagtc aggaaacttg cttgcaggtg
    28381 gatacaatcc aaaggactat tccggaatta cgtgaaaata cgcgcctggt acctaccaat
    28441 aatttgcatt tgacgttgaa atttctgggc gataccgatg aaaaagttat cccgactatt
    28501 tctcaggcat tagatagtag catcactacc attgatagat ttgaatttat ttgggaaggg
    28561 ctcgggtgtt ttcctaataa ccacaagcct cgcgtattat ggctgggagt cggtcaggga
    28621 ttagagcaaa ttcaaaaatt gactcttgca attgaatcgg ttttagagaa tttcggttat
    28681 caaccggaaa aacgcgaatt tagaccacat ttaaccatcg ggcgggttaa aaatcctttt
    28741 aaaaaggttg ccggactgga aagtttttta aattataaaa taaatcctac cataaatccg
    28801 gtaaaggatg taattttctt tgagagtaat ctgacaatgg gaggaccaat ctacacacgc
    28861 ctggccgtgt ttaatttaaa ataaaataag gagagaaaga tggcggaaaa aaatgatgat
    28921 aaaataaaag ctctcgattt agccataacc cagattgacc gtcagtttgg caagggttcg
    28981 attatgcgtc tcggagatga ctcgatgaaa atcaccgtgg atgtaattcc gaccggttgc
    29041 ttatctttgg atgcttgtat cggggttggc ggtattccgc gtggtcgaat caccgagata
    29101 tttggaccgg aatcgtccgg gaaaacgacg ctggctttgc atatcatcgc tcaggctcag
    29161 aaaaccggtg gctatgcggc ttttattgat gccgagcatg cgctcgaccc ccaatatgcc
    29221 gctaaactgg gagtaaatac ccgcgattta ctgatttcgc aaccagacca cggtgaacag
    29281 gcgctggaaa tcactgaaac tctggttcgt agtaatgcta ttgatgtgat tgtgattgat
    29341 tcggtagccg ctctggtgcc gcgcgccgaa ttggaagggg aaatgggcga ttcgcatgtc
    29401 ggattacaag cgcgcttgat gtctcaagct cttcgtaaat tgaccggtgc agtcagcaaa
    29461 tccaacacgg cggtgatttt cattaaccaa attcgggaaa agattggggt gacgtatggt
    29521 aatcctgaga cgaccaccgg tggtcgggct ttgaaatttt atacctctgt ccgtcttgaa
    29581 attcgaagaa tttccacgat caaagaaggt aacactaatg ttggaagtcg cacgcgggtc
    29641 aaggtggtta aaaataaaat ggcgccacca tttaaagaaa ctgaatttga cttgactttc
    29701 gatcagggca tttcgtatgt tggggatatt ctggatacgg ccgtcgaggc tgggtttgtc
    29761 caaaaaagtg gagcctggta ttcgtatgga gatgaacgta ttggtcaggg acgcgaaagc
    29821 gctaaaggtt acctgaccga aaataaagaa ttgcttacaa aactcgaaca gcaggttaaa
    29881 acttatctag ggctaatcaa ggaataaatt ccttttaatc cagaaagaaa taaaatgtcc
    29941 tcagactttt cgtccgaagc cgagatatta aaagttgtaa ccaagatcga atcgcagaag
    30001 aagaaatcgg cgcgcattaa tatttatctc gataatgaat ttgctttcgg cattagtgct
    30061 gaaactctta acctttttcg gatttgcgtt ggtcagcaat tgacggaaaa gcagattaat
    30121 atgattcagc aacacgaact attcgaacag gcgaaagaag cggcaattag atatctggga
    30181 cttagaatac gcagtcaaag ggaactcgaa ttatatctta cccgtcgcca gaaatatcct
    30241 ttgacggtgg ctagccgggt aatcaagtat tgtacggaaa gaggctatct ggatgataaa
    30301 gttttttgcg aaacctttat tcgtgatcaa ctggaattga atcataacgg aattcggaaa
    30361 attaggcagg ctctggttgc gaaagggata gatcgtgagg tgattgatga ggcgattaaa
    30421 aacttagtga aagatgaaga tcaattgaag gcggcttgga tcattggaca aaaaaaagcc
    30481 tcaactctca aaaacgatcc gaagcgtcgc gataagttat atcgttacct aattcaaaaa
    30541 ggatataagc ccgatgtggt tatcaaagta ctgcaaaaaa ttctatagaa ataaagtgct
    30601 taaagtttgc cttcgccaat atctgcttga ttataagccc gattccagat aactttcacc
    30661 ttcatattta tgcttaatga agagtaaatt tgaaatatga ttttttgcat ttataaagtt
    30721 tacaggagta taattgaaaa ccgccaacga cgtccgtcgt gagtttttag aatttttcgc
    30781 tagccacggt cataaaattg ttcgcagtgc cccggttatt ccgcaatctg acccgacttt
    30841 gctatttact aacgccggaa tgaaccaatt caaaggcata tttttaggcc aggagaaaat
    30901 cgaaagcccg agaatcgcca attcacagaa atgtatccgg gttagcggta aacataacga
    30961 tctggaagaa gtggggaaag acacttatca ccacactttt ttcgagatgt tgggtaattg
    31021 gtcatttggt gattattata aagcggaagc cgtgcaaatg gcgtgggaat tgttgacgga
    31081 agtctggaaa ttgccgacgg aaagattgtg ggtgactatt tatcaagatg atgaagaggc
    31141 gcttcaagcc tggcagagaa tagccggact cgaccagagt agggttcttc gctttggcga
    31201 aaaggacaat ttttgggaga tgggcgaaac cggtccctgc ggtccttgtt ctgaaattca
    31261 ttattacacc ggtccccatc cggagcacca gcaagcggaa atgattaatt ccggcaaccc
    31321 ggaatatatc gaactctgga atctggtttt tatccagttt gatcgtgata aatcgggaga
    31381 gttgaagcct ctgccactta agcacgttga taccggagcc ggattagagc ggatggtagc
    31441 tgttttgcaa gggaaaaagt caaattatga taccgattta tttaagccga tcattgaccg
    31501 gattgtatct ttaaccggca aagaatatca tacggaaaac ggtgtgcccc atcgcgttat
    31561 tgccgaccac gttcgaatgc tgacgtttgc gattgcggat ggcggtttgc cgtccaatga
    31621 tggccgcggt tatgttattc gccgcatttt gcgccgggcg gctcgttttg gtcgaatgct
    31681 ggaaatgcat aagcctttta tttatcagtt agttgatagt gtgattgagg tgctcggtgc
    31741 gacttaccct gaaattgcag aacggcgtaa tcatattcaa gcagttatta aagccgagga
    31801 gaagtcattt ggtttgactc ttgaccgtgg attggaggtc tttgctaaac tcaaggaaaa
    31861 cctgcaacga aataatatca gaataattcc tggtgaagaa gtttttaaac tctacgatac
    31921 atttggtttc ccaatggatt tgactcgcct attggctgag gaagccggac ttagcattga
    31981 tgaaaaggga tttgaaaact taatgaaccg ccagaaagaa cgtgctcggg cttcggtagt
    32041 gtttcaagcc gatgattttc tggaaaatga agattggcaa acgctgacga ctggcgacga
    32101 ttctgagttt gtcggctatg aaaatctgga aacgcaatcg gtgattcgca agtacctgat
    32161 cgaaggtcag aaagttcatt tgattctcga ccggactccg ttttacgctg aggctggggg
    32221 cgaagtcggc gatgtcggaa ttatcgaggg caataatttc agaatcagga ttgacaacac
    32281 gcggaaaatt ggctcttcca ttatccatag cggtcatttt gaaaaaggga attcaattgt
    32341 tgacccgatt gtcaaagcct gcgtggacaa cgtcgctaat ttgcagattc gcgccaatca
    32401 cactgctacg catcttttac aggcggcttt acgtcagatt ctgggtgagc acgtccatca
    32461 atccggttcg ctggtgacac cggaaaaatt gcgttttgat tttacccatt ttactaaaat
    32521 gaccaagtca gaaatagagg cagttgaaga ttgggttaat gcccgcatcc gagaaaattt
    32581 gccggtgaat gctgaagtga aggattatca gagtgctcgt gaatcgggtg cgatggcgtt
    32641 gtttggcgaa aagtatggcg atgtcgtgcg agtaatcaac gttggtgatt actcaaaaga
    32701 gctgtgcggc ggcaaacacg tcaatcgaac cggtgatata ggctgtttca aaattattag
    32761 cgagacggcg attgccgctg gtgtccgtcg gattgaagcn nnnnnnnnnn nnnnnnnnnn
    32821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32881 nnnnnnnnnn nnnnnnnnng aattaaagga aatcggtgat ctggtgcgcg ctaaattgca
    32941 taacggcgtc ggtgtaatag ccggtataat tgatgaaaaa ccgaatatta ctgtggtaat
    33001 caccgaccat gccatcaaac gatatggact aaaggctgga gatattgttc gggaattggg
    33061 gaaaatttta ggtggcggtg gcggcggtca ggcgcatctg gcgacggctg gtggccgatt
    33121 taccgacaaa attccagaag ctctgcaagc ggttattcca ttaattaagt caaaaattga
    33181 gtctaaaaat taaaagggat ccagtatgga gaaattaatt tatgagtatt ctcaatctgg
    33241 taaagaaggt gtttctttac cgcaaggtat tgaatcggca gtttctcccg ctgaattaat
    33301 accagccgaa cttctgcgcc agagcgaact acaattgccg gaagtcagcg agccggaagt
    33361 tgtgcgccat ttcaccaatt tatcaacaat gaatcatcac gttgacaaag acctttatcc
    33421 gctaggttcc tgcacgatga agtacaaccc gaaagtcaac gatcggagtg ccgctctttc
    33481 cggactggct aactgtcatc ctctccagga tgtcggcacg gttcagggcg ctctgcagat
    33541 tatttacgaa ctggaacgat atttgtgtga aattaccggc ttacaagccg ccacacttca
    33601 accagcggct ggttcgcatg gcgaattgac cggtgttttg cttatgaaga aatattttga
    33661 tcagaaagga gaaaatcgcc agtttatctt gattcctgac tcggcgcatg gaacgaatcc
    33721 ggctagtgcg gcatcggcta attataaaac tatttcattg aaatccaatc aacgcggttg
    33781 tattgattta gaagacttgc gcgctaaaat gaatccctcg gtagccggtt taatgctgac
    33841 caatcctaac acgctggggc ttttcgagga ggaagtagct gaaattaatg caatagttca
    33901 taatcagggc ggtttggtct atatggacgg tgccaatctg aacgccctgc ttggtattgt
    33961 gcggccaggc gatatgggct ttgacattac tcacatcaat ttacataaaa cctttgcgac
    34021 gcctcacggt ggcggcggac ctggtagcgg cgcaattgcc gtaaatgaaa aactggtcga
    34081 atttctcccc gtaccgcgca ttgagaaaaa tggtgataga ttttcactaa attattgtta
    34141 tccgcgtaca attggaaaag tacttggatt ttatggaaat ttcgggataa tcgtccgggc
    34201 atactgttac attcggatga tgggtacaaa aggcttgcaa gccgtttcgc gcaacgccat
    34261 cttgaatgct aattacctgc gccaaaaatt gagttctgta tttgaaattc cttataatcg
    34321 gacctgtatg catgaatttg tcgcttccgg tgaacgccag ttacaacgtt ctggtattaa
    34381 aacacttgat atagcaaagc gtttgctgga ctatggtttt cattctccga cggtttactt
    34441 tccactgatt gtcaaagagg ctttaatgat tgagccaact gaaaccgaga gcaaggaaac
    34501 tctggatcgc ttcatcgaag cccttgtaaa aatagaccag gaggcaacta atgaacctga
    34561 aaaattacgt aatgctccct ggacgacgcc ggtcagacgt ttagatgaat taaaagccaa
    34621 taaggagtta aacatatgct gggggaagta gagcgccaaa ttgtcaatac cacggatgcg
    34681 ccgcgtgcca ttggacctta tagtcaagcc gtaatttccg gaaattttgt atttacggcg
    34741 ggacaattag gactcgatcc agtcgctgga aaaattgtct ctaatgattt tgaacagcaa
    34801 gtgcgtcaaa taatgcgcaa cctaaaagcg attttacagg cggctgagag cgatttgaaa
    34861 aatgtggtga aattcacggt atttgtaacc gatttagata attttgggct attaaatcag
    34921 gtttttggcg aatattttcc agaatcagcg ccagctcggt cagccgtgca ggttagtcgc
    34981 ttgccactgg atgggttggt tgagattgaa gccatagccg ttaaaagggg aaaataattg
    35041 ccgtacaaat ttgccgttat cgtaaatccg gttgccggga aaggtaagac agttcacaac
    35101 ctgccacttt tgaaggaaat cgcggctaaa acgaaatcgc gcttcgatta ttttattacc
    35161 gaaggaccgc ttcatgctac taaaattgct gaccggattc atcaggaata tgatgccgta
    35221 gtagcctatg gcggagatgg gacggctaat gaagttatga acggtctggc gggaactgaa
    35281 acaccgatcg gcgtgattcc cgaaggaaca ggaaatgact ttgctcgttg tatcaatgtg
    35341 ccgcataaat ttgaaagagc catccagatt ttagatgaat ataaaacgcg cttaatggac
    35401 ctcgggacaa tcggcaatcg tgtttttatc aatggggtgg gagtgggttt tgacgcctac
    35461 gttaatgtga aaaccaagag ccttaaaatg ttgaaaggca gtatggctta tctatactcg
    35521 gtgctatcga gtttggcatt ctggaagtcg cttccgttgg caatcgaaat tgacggctgt
    35581 ccgataagca ccgatcgctc ctttttggtg gctgtcggca acggctgggc atgcggtggt
    35641 ggtctgaaac ttaatccgca ggctaatatt catgattcgt tgttggatgt atgcctgatt
    35701 ccggaaatgc cggtctggaa attattcctg aatctaagaa ggctaaaaga cggcacaata
    35761 gcggaagttc caggagtttt gacgatgcgt tgtcaagcaa ttaatatttc cagtgaaaaa
    35821 ccacttccgg tgcatttcga cggggaaata ttcgatgccg gtgccggaga agttgatatc
    35881 aaaatagttc ctcaatcggc ttcagtcgta ggggcttggt aataggatta aaagctggat
    35941 ttgaagcgta tttttctggt ggcaggatta ttaataatat tgaagagcaa tttgcaaagt
    36001 gctagcccag ctgatacact tatgaaaatt tcaccgaaag gggcatttat ccgttcggca
    36061 ataattcccg gctggggaca agtctataat aagaaaccgg tcaagggggt agtactgttg
    36121 tcagcggagg ggtatttcct ctataatttt ttctattata atcgaattta tgactatgtc
    36181 aaaacgacga aggagacgct gggagtcgaa acctgggtag ggttgaccga agaagagaaa
    36241 aaagcccaag tgctggcggt tactggttac gaacttacgc tgaattcctg gcgtcccaga
    36301 gaaaagcgta ataaatatgg ctggtggagt ttaggcactt acattttttg tttactggat
    36361 gctgttgttg atgcccattt gataaatttc cctaagggag agattgaact gggtagcact
    36421 gaaatcggac taaatttatc agtcagggtg aatttttaac atgaccaagt cacgtgaaca
    36481 atggggctca aaaattggat ttgtacttgc ggccgctggt tcggctgtgg gactgggaaa
    36541 catttggaga tttccttatt tggtcggtga gaatggcggt gcagtattta ttttcatcta
    36601 tttgatttgt gttgcggtta tcggtttacc cgtcattatt agcgaagtta taattggacg
    36661 aaatgctcag aaagatccag tcggtagttt taaaaaacta tcaaataaca atcccttctg
    36721 gaagggagta ggttttctcg gagtttttac tggctttatt atcctctcat attataatgt
    36781 gattgccggc tggtgtctgg gatatattgt cgaagccgtn nnnnnnnnnn nnnnnnnnnn
    36841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36901 nnnnnnnnnn nnnnnnnnng tggctctcgg gcaggcattt ttttcactta gtattggtat
    36961 gggggtgatg attacttacg gtagttatct atcaaaagaa aataatatcc cgtcgcaagc
    37021 aacccggatt gtggtaattg atacatctgt ttcgctttta gccggattta tgatttttcc
    37081 cgcaattttt gcgctggggc taaaacctca ccaaggacca agtttattat tcaatactgc
    37141 gccgatttta tttaattaca ttccgggtgg atactggttt cggattcttt tctttatctt
    37201 attagcctta gccgccttga cctcgacgat ttctttactg gaagtcatca cagcatattt
    37261 tgtggatgaa tttaagttaa atcggaaaat gatagcaatt atcggaggtg ccataagttt
    37321 attacttgga attcccagtg ctctctcctt tggtcgctgg tcgagtttcc agattatgaa
    37381 cctttccatt ttcaattttt gtgattattt ggcttctaat atttgtctgc cgataggtgg
    37441 tttattcata actatatttg ttggttggtt ttggggcaag aataatgctt tgttagaaat
    37501 gcggcaaggc gctgatagaa caccgaattg gtttttttct gtttggcgat ttttaattcg
    37561 gtatgtagcg ccggttttga ttgcaatggt tttattaagt tcactcggaa taatatgaaa
    37621 aattggtggc tattgttatt gatatttgca attggcggta ggcttttagc cgtcccacca
    37681 gttggagaaa taatcaaaag taaaccggat gatgaatggt ttgcgatgga caaactgaaa
    37741 catttcagca cctcgtttta tctgactaca acgctttttt atagtcaaaa ccggattttc
    37801 gatttaaaga ctgaccaggc taatcgaaac gccgccggct tgacgatttc tttaggatta
    37861 ttgaaggaaa taagagattt aaaacaaaag gaaaattact tcagctggcg cgacctggcg
    37921 gctgatattc tgggtgctgg cgccgcgata ttgttcatta acggaattga atgaaaacct
    37981 tgaacgaata tattggttgt attcatattc ataccgtcta ttccgatggg agcgggacgt
    38041 atgaagaagt tattcgagcc gctcaggaag ccggattgga ttatataatg gttagcgacc
    38101 atcaaacttt gcgcggtcga gatgaaggat atgccggctg gcatgctaat ttgtttgtta
    38161 gcattggtta tgaaattcag gatttagaag atcgccatca ttatttagtg tttggtatnn
    38221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ctacaaagct ggcgattgat
    38341 ataccagaag gcatttatcg catcgaagcc aaacgcaatc agcgtggttg gatatattca
    38401 aatcacataa aactaattaa tggatgaaaa atgtgtggaa ttgtaggaat ttataatcat
    38461 gaagaagccg ctactctaac ttacctcgga ctttatgctt tacagcatcg cggtcaggag
    38521 ggagccggta tcgcttcgtt cgacggtaaa gtcagtcatc ttcagaaagg caaaggactg
    38581 gtcgccgatg tttttatcaa tcgtgagcaa cttatgcatt tatcggggaa gacggctatt
    38641 ggacatgtcc gatattcaac ctatggaagc aatggtaacg aaaacattca accattagca
    38701 ttcaagtaca aaggcagtca gattagcatc gttcacaatg gcaacctgat taacctggat
    38761 gaaatcggcc gagatttgga gagtcatggg gctttgtttc aaacgacctc ggacaccgaa
    38821 tatataatac atttatttgc tcattcgggc ggtgattcgt tggttgagaa atttttacag
    38881 gttttccgtc ggataaaggg tgctttctcg gttttaattt tgtttgacga caaaatgata
    38941 gtggctcgtg atcatcgcgg attccggccg ttatgtattg gaactctcga tggttcatac
    39001 atttttgctt ccgagacttg tgctttggat ttaatcggtg ctacttatga gcgagatgtc
    39061 aaaccgggcg aaatgatagt attggacggt actaatgtta aatcttatca aatcgctcca
    39121 cctgagccga agcattgtat ttttgagtat gtgtattttt cacgtccgga tagccagata
    39181 tttggcgaat atgttgataa aaccaggcgc aaattgggta aaattttagc tatggaaaag
    39241 cccgccccgg aagctgagat cgtgatttcg gtaccagact cgagtaatac tattgcgctc
    39301 ggttattctc agcgttcaac ggctcggttc gaaatcgcct tgattcggaa tcattatatc
    39361 ggacggactt ttatccatcc cacccagact atgcgtgatt ttacagctaa aattaaattc
    39421 aacccggtcg ggggagtatt gaaagatcgc aaaattgtcg tcgttgatga ctcgattgtg
    39481 cgcggtacga ctttacgtaa attagcgcga atgttgcggt ccgccggagc taaagaaatt
    39541 catatccgca ttggttctcc gccagtcaaa tttccttgtt attatggtat ggattttccg
    39601 tcgcacgaag aattaatcgc caatcagaaa tccattacag aaataaagga ctatttagag
    39661 gttgacagcc ttgaatatat aagcccggaa ggtttgttaa atgcgatgag cttaccggct
    39721 gaaaattttt gtacagcttg ttttaccggc cattactgtg aaaaaattaa ccaagagaag
    39781 gagaactcat gttaaaagtt ccaagtaaat caattgcctg gcttgattac ctgtttattt
    39841 tccgaccgac cttattcttt gcggtctgga ttatcacact tgccggttac agcggctatt
    39901 ttattttcag cgagaagcaa ccagcctggt ggtcattaga agtcaactgg aaactggtat
    39961 taggttttat agttgttaca atggcgacgg gagcgacttt tatcttaaat caactgcacg
    40021 atatcgaaag cgataaagaa aaccgtaaac tttttttaat ttcagaagaa catatcaaag
    40081 ctgatttagc cttgaaaatt gcactaataa ccatcggtgt ttcattggta ttagccttga
    40141 taataaactg gcagttcttt attgcgttgg gaatttgtgt ttttttctgg ggttatttgt
    40201 ataattataa gccatttgaa tggaaagaca agccgatgtt gggcgtgctg gtcaatttta
    40261 tcagcggctt gtgtcttttt atttgcggct ggatcatcgc cggtaaatgg caattgaaag
    40321 acattattta tgcgataccc tatttattag cttggacggc agtagcggta ctgacaacta
    40381 ttccagataa agaaggcgat ctcaaaacag ggaagaagac catctcggtt cattatgaaa
    40441 atagagttac gatttggttg gcattcattt gtctggtttt gagcttcgca ctcgggatgt
    40501 attttaatga tccggttatc agtttatcct gtttgctttc atcaccactt tttattatta
    40561 tgctttttaa gccgactgaa gcctgggtct tgcgatctat tcgctatcca atcttgttcc
    40621 tggctttgat tttatgcgtc gagttccccc tattttttat tgtactattg ataaactatt
    40681 atctttgccg attttattat ttatctcgat ttaatctgaa ttatccgaca tttcaagttg
    40741 aggaatcagt atgatagaag ttaacccaga ctgttgcgat ttatgtggga cgtgtatttc
    40801 ggtatgtccg aaggattgta tcacattgac tgaatcgcgc ctgatagttg atgattccca
    40861 atgtaatgct tgtcagattt gtgtgagagt atgcccattt aaagcgctaa ggctggttga
    40921 ataggaaaaa ttatgcgaaa agaatatgat gtgattgtcg tcggtggcgg ccctgccggt
    40981 agtacggcgg ctcgtttcgc taaaactgct actaatacag tcgctctttt tgaaagagat
    41041 cgggaaattg gtattccggt tcgatgtgcc gaagccacct cgattccgga tctcgaacga
    41101 ttcgtaaaaa ttgatccaca ctggatagca actaccatta ccaaagcccg cttaatttct
    41161 ccatccgacc aaaaggtctg gttgaatttg cctaatgatg gcgtaatcct taaccggcgt
    41221 ttattcgata atggattagc cgaaatcgcc gctactcaag gagttgaaat attcactaag
    41281 gctaatgttt ataaaattgt atcagatgga gagcaacact gggaagtgtt tgtgcgccac
    41341 ctcgaccgcg attatatcgt gaaatctaag ataatcatag cggctgatgg ggtggaatcg
    41401 cgaattgccc gctttgccgg aatccgcact cagaccgttt taaaagatat tgaatcctgt
    41461 gcccaggttt tacttggcaa tctggatgta gctgagaatc aaatagattt ttatttttcc
    41521 agccgctggg cgccgggtgg ttacgcctgg gtatttccca aaggcaacca caccgccaat
    41581 gtcggtttgg gcgtcaacgg cgcttattat aagggacgct cggcgattga atatttggat
    41641 gattttatca atgaaaaatt tcctgaggcg gcgcgattaa ccacggtcgc tggaggagtt
    41701 cccgtcgcca aatcgctaaa aaaaatcgcc tcagatggat tactaattgt cggagatgcc
    41761 gcccggcagg tcaatccggt aaccggcggt ggaatcttgg cggcaattac agccggtaaa
    41821 attgccggtt tggtcgccgc tcaagcgatt gcccgcaatg actggagcgt aaatgtattg
    41881 aaagaatatc agacggaatg ggataaaaat gtcggcaaag attatgcgcg cttttataaa
    41941 ataaaggaat ggatgaccga gcttactgac gacgaactcg atgaaattgc caccttactc
    42001 caaaaaatcc ctcccgacga tattaacatc atgaccgtct tcaaaattgc tttacgtaat
    42061 aaaccggctt tattgcttga ggcgatcaaa ttatttgctc aaatgatcta atggacgatt
    42121 ttttaactct cgccggatta aacgaattta tcgccctcga tgttgagacg accggcttga
    42181 atccgcaaca agatgccgtg attgaatttg cggcggtaaa atttataaac ggtaagccgt
    42241 ccaatacctt gaattttctc tgtaatcccg gttttcccat ctctccggaa atcgagcaac
    42301 tgaccggcat caaagatgaa atgctaaaag ataagttgcc ttttgaacaa catcttgatg
    42361 aagtcctgga gttcatcggt agaattccat tggtcggaca taatatcggt tttgatctcg
    42421 gttttttaaa agaatatctc aaaaatttca aaagccagcg gaaaagaatt ctatataatc
    42481 cgttatacga taccttgctt ttggcgcagg cttttctctt ttttttatat aatcatcgct
    42541 tgtccactat cgctgagttt tatggattaa gtgccaaggg cgcacaccga gcggagaatg
    42601 atgctctcaa caccggcttg atttttgtca aattaatcga agaagtgcag agttacgacc
    42661 tggagacgat ccagactatc aatatggtgc taaaagatac cgacgaccct aataaattac
    42721 tttataagaa tgccgccgaa gctctcttac ttcagagaag cctcggctct gaagaagcac
    42781 ctcaaatcgg ttgggatccc ccagtaaata tttttggtca gtctattgaa aaaaataaag
    42841 aaagcgcaaa ggcttcctta aaaattgaga agttttttga accggctgga ccaattgccc
    42901 agaacttcgt tggttttgaa gaccgaccac aacaaaagga aatggcgcaa ttggtgctta
    42961 acagtttgca ggcaggcgat tcagccatta tcgaagccgg aaccggagtc ggtaaatcac
    43021 tggcttacct aataccggcg gtagtttggt tgaatcaaca gaagaaggag aaaaagcggg
    43081 tagtaatcgc cagtaacacc aagactctcc aggaacaaat attttataag gaaattccct
    43141 ttgtatcgga aaaattgaaa ctgccattca aagccgtttt acttaaggga cgttcaaatt
    43201 atatctgctt gacgcgctgg catcgttatc taaatgctct gcctcaacag cttcacctgg
    43261 cggcgcgctc cgccattatt ccaatcataa tttggcttaa gcatactcgc accggcgaca
    43321 ttgcagaaaa taatggattt aatctggcgg cgcattacta tatctggaac gaaatttgtt
    43381 ccgagcccgg ctattgtact accagtgttt gccagaaata tggcggctgt tttctgggca
    43441 aaatacgtta tcatgcgatg aatgccgaca ttatggtagt taatcattcc ctgcttcttg
    43501 ccgactcagc cgccgataac cagattctac ctggatttga tgctttgatt atagatgaag
    43561 ctcataatct ggaaaagaat tcctataatt attttgcgca aaaagtcaac ctaccaacgc
    43621 ttatttattt tctaaacgga atctatagcg gctcggcacc tgaacgtggc ttgctggtag
    43681 atttagccga aagcgctcat cagctgaaaa gtctcgataa aatcgaaccg ctccgtcaaa
    43741 tgattctcga gaaaacctcc gatgtcaaaa ttgcagcgga ggtatttttc cgaaaaatcg
    43801 ttgccactaa attcaatccg gctaatggca atcaaaagtc ttttggcatt aaaaaacgct
    43861 ataaagattt ttcccgtgaa tttcccaatc tggaaatcga aacgaataat ctgatctacg
    43921 aactgggcac tttgtggaat ctgctgggta atttgagcaa taaaatggac gaaatatggg
    43981 gcgattatgc cgaagagtat gatgaattgc aagtgcggct aatgaatcag aaaattatgg
    44041 tcgaggcatt tctggaaacg ttgcgtaaag ttacaactgc caacgacgat tcgcatattt
    44101 tctggtatga aattgccccc aatggtaaaa acaataccgt agaaattgtt ggcactcccc
    44161 tcaatattgc gagcaacata tttgaaaaag tttataagca cgtttccagt gcgatgctga
    44221 cctcggcgac tttgcaagtt gccggagctt ttgattatgt tcttagtcga accggcttaa
    44281 atttgataga ggatcataat gttgtaagtc gcgcactcgg ttcaccattt tattataacg
    44341 atcagatggc attttttacc tatcaccaga acgataatga aattagtgaa gcatttatag
    44401 ccgcgcggtt gataaaacgc ctttccagcg aaaccgggaa gggcattctg gttttgttca
    44461 cttcttataa ttttatgtac gaggtttatc aactatgtct gccgactttt atcgaaatgg
    44521 gtgttactct tttagcgcaa ggacagggcg catcgaggac agcgcttctc gagcaatttc
    44581 gcttagagca gaattcggtt ttactgggta ccgatagttt ttgggaagga gtggatgtgg
    44641 ttggaccggc attggaaatt ttagttatca ccaagttacc gtttccggtg cctaccgaac
    44701 cgattatcga agctaatgtc gaaaagttga aagccgaggg gcaagatcca tttaacgaat
    44761 actatgtccc ggaatcggtc ttgaagtttc gccagggagt tggtcgctta attcgctcaa
    44821 ttaatgatgt gggagtcgtt attaatcttg acagtcgcat tgataagaaa gcctatggcc
    44881 gctacttcaa aaaatcgtta ccggttgagc caatttcaat tgttggcgaa gatgaattat
    44941 tagccgctgt ccgcaagttt tttagaatag gaaaatgaaa tgaggatatt ccggatttgt
    45001 ttatgttttc tgctaagtac ttcagtaatt ttggcggaga actccaagaa attaaccgtg
    45061 cgggaaatca ttgatgatcc gggattggat gcgagaactt accggcagaa acaatttacc
    45121 tggactccga agggcgacgg agtattattt ctcgaaaaaa ataaaaatca aaattgcgat
    45181 ttgctgaaat tttcgattaa ttcccttaaa attgacacgg tttttactta tgaacaatta
    45241 aggtggagta acgatactca cacggttcaa cttgatccgt tcggtttcat atcggctccc
    45301 gatggattcc gatatttact gactggtgag aatgaccttt tcctaggcga tgaacgcgat
    45361 ggaactatag tgcgtttgac aaatgacgga acccaaaaaa gtgttccaaa attctctccc
    45421 aacgggaaat gggtcagcta tatttgcgac cataatatct ggattgtcaa ctgtgaatcg
    45481 agaagctcgc tccagttgac aaccgacggc aatgaatcaa tttttaacgg tgaatccgac
    45541 tgggtttata ccgaagagtt tgattttgaa caagcgtatc annnnnnnnn nnnnnnnnnn
    45601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45661 nnnnnnnnnn nnnnnnnnnn ngtggttatt caatcaatta atcggcttca aaataaccag
    45721 aaagttacct atggtaatcc gcgaaccggc cagacaaaag ttattctcga agaaaaagat
    45781 ccgtattggt taaatattac ggacttatac tattttatga aaaattcgcg caattttatc
    45841 tattattcgg aaatagacaa tttcatgcat ctttatctct atgattttga aggcaatttg
    45901 atcaaaccga ttactagcgg cgattggatg gttaccgaat tgaatggcgt aaacgaaaaa
    45961 gacagtctgg tttatttcac agcgactaaa aattcggtac tcgaacggca tatttatagc
    46021 gtgcatcttg gctccggtgc cattagtcag attgattccg gtgatggagt gcactcggca
    46081 gttttttcac cggatttgaa atattatctt gattttttct caaatgttaa ctcgcaagta
    46141 gatgtcagaa ttattcaaaa taatggcaga aaaattacgg acttatatca taataatgat
    46201 ttcagtccgc aaaaatataa tttcggcgtt acgcgtttta tcaagatacc ggcaattgat
    46261 ggggattctc tttatgctag cctattatat ccgagtgatt ttgatccgca aaaaaaatat
    46321 ccggttattg tttatgtcta tggtggtcct ggagtgcaag tcgttcaaaa ccgctatgga
    46381 ggtgcctgga cccatttgtt agcacaacag ggttatttaa ttttcagcct cgacaatcgc
    46441 ggttcgtatg ggcgtggacg atattgggaa cgcaggattt accagcaact ggggaaaata
    46501 gaattacagg atcaactgga tggggtgaaa tatttaaaaa gtctgccgta tattgattca
    46561 ggaagaatcg gaatctgggg tgccagttat ggtgggtata tgaccttgta tgcccttacc
    46621 aaaacgccag aggtttttaa aaccgggatt gctatttctc cggtgacgga ttggaaatat
    46681 tatgatgccc catatactga gcgatattta ggattgccca ttaatagtca aaatatttat
    46741 aaagaaagtg cgccgcttaa ttatgttaat cagataaaag ctaatttttt actaattcat
    46801 ggcacttatg atgataatgt ccacttacag caaagtctgg aatttattga tgaactaatc
    46861 agcaacaatg tccgttttga gttgatgctt tatcctggac gaaaacatgg aataaccgac
    46921 cgtgaagggc aaattcatat gtacgagact tttcttgagt tccttcagag aaatctttga
    46981 ccgatttagg taatgatgtc agctaatcga aaattatcgg attctctaaa attatggtta
    47041 agagcatatc gggctccttt tctgactgcc agcgttgcgc cggtaatttt aggtacttct
    47101 gtaggttggt atgagaacgg tagaattaat tttgtttccg gtttactggc tttattagga
    47161 acgatttttg cccactttgg tgcaaatatt tttaatgatt attacgatca cctgacgggc
    47221 tgtgatgaca ataatctcga ttttactcca ttctctggtg gaagtcgcac gatccagaac
    47281 ggcctattga cggcacgaca ggcattttgg gctggtttag tagcattctt gttggtagtt
    47341 ctaattggat tattactaat tgtctatact ggtcgcccgg ctttaattat ttttggaata
    47401 gtcggattgg caatcggttt tttgtacact gcgattcccg tcaagttggc atatcgcggt
    47461 ctgggagaaa tggggatatt tattgcgttt ggtccattga tagtaatagg agcatcctat
    47521 gtccagactg gcacaatttc ctttttagcg ttcttagctt cggtaccggc tggattattg
    47581 gtgatgttga ttctatatgc caatgaaata ctggatgaga aagccgaccg ctcggctgat
    47641 aaaaatacaa ttatcgtaaa aatcgcgaat cgtaatacgg ctctgaaaat atatcgtttg
    47701 attttatatg tggtgccgat ttggatttta gtttttgtaa ttgtcggata ttttccatac
    47761 tggtcgttga ttattctctg ccttatgcct aaatacattg gaactatttt tctctcgaaa
    47821 aagccgttca atgatgttaa ggagatggtt cctctcagtg ctaatacgat tcagctccaa
    47881 tttctgatga ctattacgct ggcgatttcc tttatattgg ctggactcac cggttgattt
    47941 atttgctcat ttcgtcgttt ggggtgtaaa tttagtccgc tttttgtaaa aattgggaag
    48001 tcgtccaacg gcaggacatc agactctgga tctgagtatc ggggttcgaa tccctgcttc
    48061 ccagcgaaag ccgcctcggg cggttttttg aaatggcgca ttcgtctagt ggcctaggac
    48121 gctggcctct cacgtcggta acaggggttc aaatccccta tgcgctacaa atgtggtcgg
    48181 tttacgtaat acgtagtcta aaagatggtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48301 nnnnnnnnnn ttttttcgtt gatgatattt tcgaaataac cagctgggta ttataccgct
    48361 aagcagacgg ctgaaaattt cccatccgga atctattttt actaatggaa tcctgggaaa
    48421 tatagccgga ctagaagatg tgttttacag aaaaataact gatattattc aacaaaagaa
    48481 gtcattactt tactatatca gtagttcttg tctttcacca gatagatcat cggtaggcta
    48541 atgatagtta tagcgccttt caaaatcaga ttggcgaaga aaattgacca aacgacagac
    48601 ggattataaa ttccaccaaa cgcgccccat acaaagatta ggctatcaat cggaatgctc
    48661 aatgaattgc ttactaaaac tcgcgcccac tgatgacgtt tagtgacttt ttcaatccat
    48721 atgcgataaa tctcggtatc taataattcg gcaatgactt cggcgattat gctggcaata
    48781 acaattcgcc acaccggcga aagtacctta gcaaattcta attgtggacc tacattcgga
    48841 tcgccgggta tataagagac tatccaaaac aatagtgcca ttaatagatt aatcaccgcc
    48901 gcgcatataa taactatacg tgtgcctttc aatccgatag ttttatgcac aagatcacgc
    48961 agggtaaaag taaatggata tatcagtgta ccggcatcga ttgaaaagcc taataaagaa
    49021 attattttca aacttgtaat atccgacagc atttgagccg atatataagc ggcaatgacg
    49081 actatcaccg aaattttgat tgcttcatta ttcaacgaag tttctttttc caagtgcacg
    49141 gttcaattta aaattatttt tctaatagga gaactatttt ttgtctgagt cgtaaaatag
    49201 cgtgatttta ttgattattt ctaaatctaa acaatcataa ttgaaaaata acttcctcct
    49261 cctcactcgc aggtctgctc tccgcagacc ttttttattg aaaaatccat tggtaaaatt
    49321 gttactaaaa cattatttta gaacatgatg gaaaataatt ttgagattaa accgttcgtt
    49381 ctgagaaata aaattcaaca ctatgaatgg ggaacgcgtg gcgagcaggc atttatccca
    49441 cgtttattag gaatacagtc tgaaggaaat cgcccttatg ctgagctctg gatcggcgcc
    49501 catccgtcgg cgccttcaca ggtaaatatt tccaatgacc ttattgattt gactgaattg
    49561 atcaaaaaac atcctgttga aattctcggt gagaccgttt ttaaacggtt tggagccaat
    49621 ttgccatttt tattcaaagt cctatcagca ggcaaaccac tttctattca agcgcatccc
    49681 aataaatcac aagctgttcg attacatcag cgtgacccaa tccattatcc cgattccaac
    49741 cacaaattgg aaatagcaat agctatagat aatcttaaag ctttagtagg gttgctaaat
    49801 attgttgaat taaggaaaaa actcgaattg tatcctccaa tccaatcttt tacagatagg
    49861 gtattgccag gcgatagtac aatttcagaa gcggatgaaa ccaccatcgt caaacagttt
    49921 tattcaacct taatccggaa atctgttggc gaaccgcaat tatacggaaa tttagtcgaa
    49981 tcccttgcag aaagcatttt tactcgtggt aatcgaattt cggaacagga acagctgttt
    50041 ttagaattaa aaaaatgcta tccatcttcc gatgttggtt tgattttaat attattaatg
    50101 aatttggtcc aattgacttc gggacaggcg cttaatattt cggcaggtat tccgcatgcc
    50161 tatatttctg gaaatataat tgagtgtatg actaattcag ataacgtcgt ccggattggc
    50221 ttgacaaata aatataaaga
//
LOCUS       DAOMJU010000003        49852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_532_length_49852_cov_16.668956, whole genome shotgun sequence.
ACCESSION   DAOMJU010000003 DAOMJU010000000
VERSION     DAOMJU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 49852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49852
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_532_length_49852_cov_16.668956"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1539)
                     /locus_tag="PLO58_00580"
     CDS             complement(<1..>1539)
                     /locus_tag="PLO58_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73314.1"
                     /translation="GPFVGAEFHHSYMIPQRISFFYPVANSIDLSRDYWTRDSSFVAD
                     WTLKIGNSADIEIGKKPAEFDLTPYSVDFHYKDDEYIVKAAYRFCNEKPAMVVEIEIV
                     NNSGIAQDFQFDTRLRTSIRTCHTFREIDKAVSEIEDNTIYNNFYDADADSAVVFITN
                     DGDLPVECLTANVVQLPFAEFIYKKGLKPGESLNIIQIIGSSKPEEGKNLVKYLRANY
                     QLEIDQYEKYVNEQAFLSSLMKTGSPATDHSVAYAKAVMSANAHYLDGEIVPMPCPAE
                     YNFYFTHDALVTDLAAVNFDLARVRRDLDYIVRHSDENNVIPHAYYWKDGKYVTEFAS
                     SDNWNNFWFIQVAAKYLRHSGDSDFLTQLYPYLTVSVERSLLTLEKDDLMWSYRPDWW
                     DIGHNYGPRSYMTILAIKSLRDYAYISTVLGKNPEKVVELMALADKMQTALVAKLWND
                     DMGYLINYNEDGSLDSHYYIGSLLAVHYGLLDKEKQNRLVQTAMAKLLDEKVGIYNVY
                     PMDFH"
     assembly_gap    1540..1639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1640..4590)
                     /locus_tag="PLO58_00585"
     CDS             complement(<1640..4590)
                     /locus_tag="PLO58_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laminin G"
                     /protein_id="HQO73315.1"
                     /translation="MSRKPLIILFLILNMFTSIKAQQININRVEQMPNLPTPYFMRDW
                     KEVSRGYDRLVFDLNRTGQYLPLVWLNNNTVNYPNHSSFGLHTVVGTTYPSNAEAINV
                     LPAVIGATLVGIDKSDQNGYNWVLGCEEWFNRRPEENVYLNSPTAQSGDDWWYAVMPN
                     VFFYQLYDLYPGTGDFDYQFTSVADQWLRAVVAMGGSAAPWQKPNMNYRGWYLSTMKP
                     FTSGVPEPEAAGAIAWLLYNAYTKTGEAKYRIGAEWCLEFLNAFSANPSYELQLPYGV
                     YVAARMNAEQGTNYNIEKLLNWCFDPENNLRNWGVTLGKWGDYDCYGLVGEAKNEGYA
                     FAMNGFEMAGALVPMVRYDSRFARAIGKWVLNLANASRLFYSPFLPDSLQDGETWAKE
                     YDPEGYIAHESMREIHPNWGYSPFATGDAISGGWGATNFALYGSSHVGILGGIIDTTN
                     VTGILKLDLRRTDYYQKDAYPSYLFYNPYSEVKSISFNVGTQAVDLYDAVSKTFIVTG
                     ITGETNLNIPADGAVLVVLVPSGGTVRYVEEKMLVNDIVVDYHSGQTVTNYKPRIKAI
                     ASDSTALKAGRTYKIYCTAVDRDADELHYSWQINDSEIGCDSSIIELTMPELNTTLTI
                     ICRVSDEVNTPVADSIQILVYTVQNQVPIISSLTASARRIAPTDTTLINCSASDPDGD
                     SLIFNWSAASGILESDSAQAVWQAPDSSGYFLIKCTVSDLFGGVASDSILIVVGDTSG
                     HEIGAPLAYFAFAGNADDLSGFNNHGTVNGASLSADRFGKLNSAYYFNGTSDNIRVPN
                     SVSLNFTKAITVSFWINAAELYTSRESYPISHGNWENRWKVSIIPQKKIRWTIKTTTG
                     VKDLDSDKTINVNQWYHIVGLYNGIDFDLYVDGELSNHTTFSGDILTTNIDLMIGQCL
                     PAVSQYNFKGVIDDIRIYDYALSIGEISKLYTEPSAIRSDTKEMPTSYKLSQNYPNPF
                     NPVTTIKYDLP"
     gene            complement(4663..5691)
                     /locus_tag="PLO58_00590"
     CDS             complement(4663..5691)
                     /locus_tag="PLO58_00590"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO73316.1"
                     /translation="MNKSMYKKVLIFILLINSLSMGSSKRLTNVGTTAAPFLEVGVGS
                     RAIGMGGAFVGVANDVSALYWNPAGISRITSPEAVFEKVEWLADISFNYIGAVIPFGR
                     WGTAGFFLDAMTVPKMAVRTVDYPEGTGEMFDASSIAFGLSYSYALTDRFSIGLNGKY
                     IEERIWHERSHGVAFDIGTLYKTSFSGLRIGATITNFGTSMSLDGSDLIIYYDADESI
                     DGNNDRIMGKLMTDEWPLPLNMQFGLAYDLLSTKYIKMTIAADAFHPINNSESINTGT
                     ELAIYDMLFIRAGYQAIGQTNTEEGLTFGVGLRYKLFGQSYIKFDYAFADFGRLKNVS
                     RYTLRLDF"
     gene            complement(5703..7100)
                     /locus_tag="PLO58_00595"
     CDS             complement(5703..7100)
                     /locus_tag="PLO58_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQO73317.1"
                     /translation="MIKTSGLIKHFLLVSLLIDIYLFNGCAQKTEENIVAKVADRTIT
                     ADEFAFAYEMAPRSITSLTSKQARQAVLDQLIDRIVLARQAERLRLDDDTLLQRTVDF
                     YKRLAVNRELYLKHIRRLVEVSEQEERQAFEYSKTKLYVRHFSTKQFDLARQVSLGIV
                     QFEHTPIFTGAPQVELTDYGIVDKISWRDIPQEVANILYNLPLRQYSDPFFDGQRYHV
                     FQVVEFEKEVLLRESDFQANRQSLNGIIRQRKEQKLATEYVQKIMTPQNVIIKAEALN
                     RLTEEVWRNRPTEVVTLEQFITNREINTIGESSRDFLNKPIATFKSGEMTVADILINY
                     KVNPVKISYKSKMTVRESLKNAVGMYVRDWVLSEQGMKERLDRKPSVIAEERSRSEYL
                     LMRKMLAYLGREFYAQNQDTTGRQEQLEKYIANYEDNLRRAENIEIYSSNLMAVKTTD
                     EGLARKIDFVVFHTQ"
     gene            complement(7097..10309)
                     /locus_tag="PLO58_00600"
     CDS             complement(7097..10309)
                     /locus_tag="PLO58_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73318.1"
                     /translation="MNKKLFFKSLLIIMLFLGVTALAQTPSSLRGHRMYRRRGLMNGN
                     LVSTLFWNYCEVGDYPDEPSGCWPSAERHYLDDITLIVSVKTENRNGETIYPMETQFR
                     EFVDISPDGIPWGFEPQPFWFNMDEQYNTSPAMSNNPNTWPDYWPDQDMSWNGYWNGY
                     FGRGVLNADLETIFVFDDDQDKEPNVKWNFYCDERDTTRGGIGLVVKARGFQWSQVLA
                     EDCIFWLYDIKNESTHDYLKCYFAQYIDWGIGGVGGDVQNIGEYNTDLDIAFAYGPPG
                     AVGTPGNWYPIGYAGYAFLESPGISNDGKDNDNDGMIDESRVSDGPGVYISDYPYGFT
                     DAQRAKFIEVYNYEPRAHWSADEDCDWTPFTDVNGNGVWDPDEPLNDDKGKDGVGPYD
                     PQYFGPDEGEGDGIPTDPKEPNYNDTDPDESDQIGLTGFEIFPTHTYELTNDEENYHV
                     FEKSPKPVDQLLQPNNLSMFFSSGPFPLMRNSIERYSMALLFGEDRDDLFKNKKTVQQ
                     IYNSDYSFAKPPLKPNLKVYPGDGEVTLVWDDIAESSYDPFLQEYDFEGYLIYRGTTP
                     DFLESKVITDAYGNTTYRKPIAQFDLQDGKKGPHQVAIYGVQFNLGEDTGLRHTYTDR
                     DVKNGQTYYYAVVSYDYGLYHYSESGVEGLPPSECSCIIGTNSLGQVTFLDVNCGMAI
                     PRPASAGYIPPSLDGEIAHVGPGTGSIAIDLVEKHKIPSGLTTYEILFLEDSKYHTEA
                     HPWYQVRNVVADTLVLDSTYVKSSGAESPVLDGYTVTVYNDTNITLDEDKSGFIEGSS
                     DYILKVRLNPDNENVAGYRMNLELPADFLVIFHSDFYKNSLKLWSYYSCPSNVQVYNL
                     TDSVEMDYAIIDNDSSLTYTSGDDIALIIPDDSFILKRYTSWMIRLSPLFEIDTSWVN
                     GVPYADTIWTTIDPPEPGEKLFIKMKKPFREGDIFRFSLHGADSSDVLAKAELDNICV
                     VPNPYVVTASWEPANTYKFGRGERRLHFYHLPRQCTIRIYNVRGHLIDTIVHNSTADN
                     GMEPWDILSKEDNEISYGIYIYHVEAPGIGTKIGKFALIK"
     gene            complement(10321..12972)
                     /locus_tag="PLO58_00605"
     CDS             complement(10321..12972)
                     /locus_tag="PLO58_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO73319.1"
                     /translation="MKRTTILIIILVILYISLLEAGTTGKIKGRVTDKISGEPLVGVN
                     IVLEGTSQGAATDIEGNYVILNVLPGNYSVKASMIGYQTVIQKNVQVSVDLTTPLDFE
                     MVSTILEGEEVVVIAQRPLIRKDVTSTSFRVSSEQIDQLQVQNLSEIVELQAGVVQGH
                     FRGGRAGEVMYIIDGVPMNDAYAGEHLFDVESDMIQEVEVISGTFNAEYGQAMSGIVN
                     IVTKEGQEYYSGKITFYCGDHLSSHTHIFMNIDDINPLDNTNYQFNLSGPIPLLGKNL
                     SFSLLGRTNTDDGWLYGQRIFRPSDFCYFSDDPNKQIIQSTGDSSFVSMNSSNMQTLQ
                     GKLSFKLSRMDKINYTGFYEKSKYRSYDRLFKYNPDGNYRHENMSYQNSLQYTHMFNP
                     NTFLTANYSNLFTTYGQYVYKDKYDPRYVPIEYLNATNSNGFSTGGMRMWHHFRDNRT
                     EIIKADLRSQITEHQNIGAGVSYKKVHLSLHEYQIYFDENDNIQIPSDSSCYNNSYLH
                     KPYEISAYLQDKIEFGDMIINVGLRYDYFEPDGVVPTQFYNTVGAPKRKAKSSYQFSP
                     RIGIAYPISDQGVIHFSYGHFFQIPNYEYLYANPDFEVSLVQLEGSEPPSNGFNVMGN
                     AELRPEKTVSYEVGLKQAITDNLTIELTEYNKDIRDLVGQLTMTDIYGGKFWRYINRD
                     YANVKGITLAIEMLEKPGGIGFSVDYTYQSATGNASDPLDEYRNQGSDPPIESEKKRQ
                     PLNWDQTHSLNLTTTTTQKGFHICLIGKIGSGTPYTRESPYYNNRILNGERKPMTITF
                     NLNLSKDIPFKNLMISPFIRITNLFDRLNNLEVYPSSGTADYNYSMIFENYRGYKTQE
                     EWNIQPNWYEEPRNVIIGCSISFDQKK"
     gene            complement(13047..15551)
                     /locus_tag="PLO58_00610"
     CDS             complement(13047..15551)
                     /locus_tag="PLO58_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO73320.1"
                     /translation="MKKTCLLTMVVGLILLNFAFGDNLIGNGSFEDQSPAFWSPLNGT
                     FGEAVDISSTEHFAGFHSFEITKSAASSDVVGWVSDNNATLYWNNATAGTYALTGYVK
                     TVGVNTNPASDAEKVGIVYEFKNASGGELTTETVWADQSVADKDWTKLTGVAILTEAP
                     ASVVVKLVMGKEATGTVYFDNIDCNTTDTWTMGIFNYGAEDVNGWMDWYSENGSYAVV
                     TDAEAHTGSYSVVMEQPDTCSQLNELVYYSKPYPVEAGEWYKIGVWVKTENVLDSSEF
                     EPTYITKEGIPERISLCYFFHGDADIETAWDKLPGGDKFVYIDQREASTGWTHYVVAE
                     QAPAGDEPATGISVRARFNNKVTGKAWFDDFTVEKIIIDGDNLIGNGSFEDQSPAFWS
                     PLNGTFGEAVDISSTEHFAGFHSFEITKSAASSDVVGWVSDNNATLYWNNATAGTYAL
                     TGYVKTVGVNTNPASDAEKVGIVYEFKNASGGELTTETVWADQSVADKDWTKLTGVAI
                     LTEAPASVVVKLVMGKEATGTVYFDNIDCNTTDTWTMGIFNYGAEDVNGWMDWYSENG
                     SYAVVTDAEAHTGSYSVVMEQPDTCSQLNELVYYSKPYPVEAGEWYKIGVWVKTENVL
                     DSSEFEPTYITKEGIPERISLCYFFHGDADIETAWDKLPGGDKFVYIDQREASTGWTH
                     YVVAEQAPAGDEPATGISVRARFNNKVTGKAWFDDFTVEKMKVAPGTGIITDKGFGGD
                     IPVKYAIMDNYPNPFNPETRIVYSLPQSGKVNLTVYNILGQKVRNLTDTVQNAGTFTL
                     TWDARNDFGQNVPTGIYIAVLTINNQRVTRKMVLMR"
     gene            complement(15561..>17332)
                     /locus_tag="PLO58_00615"
     CDS             complement(15561..>17332)
                     /locus_tag="PLO58_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HQO73321.1"
                     /translation="ERLQYFLSICEENELYTLPTFIVGHMSGEDWGMPWLKGSDLLKD
                     RRVVDAIKFYITTVIKAINNFKYIQAWLLSNELPNFVGKQEPDDVTAWVEEIIATIRA
                     IDSQRPISIGDGAWAPEIIADQTGFHLRKLNLYQDFVGLHFYPRGMSPWHHSYTTAFR
                     IQLAKVWGKPVLVEEFGTSTTLCSEINQANYYRNVFYSALINGAQGVLNWCLNDFDFK
                     DKRPYSHHLFEERFGIIRTDQSLKPAAYEFQKLEKISSDLITGKYNKVEYNSGLFIPS
                     SYYYNYPYMFKVEGENHYDFFLECFSLLKRANLDVRMVVEPAQELENDGCYSHTLDLK
                     PEDLPVLFLPGLRFMTKQTRIALENYVRDGGVIYFSFANDSWVTDWDKLAGVVTDCKF
                     GVPDFRVKTDLTVKVENEWGKFAKNEIFTISLLNNQPALSYCPIISTTARVIMTDEYG
                     MPFLLENAIGKGKVYFTSYPLEVLALVINDDHLRAILCRIYKSINRLHGKRAEITIEG
                     DGLELGVWENQVDYRVIVLNHSWQRQNGALYIVHPPLSISSDESDLKIEEEGLYYLTL
                     PRKGVAYLKIQNELKSHKNKQQT"
     assembly_gap    17333..17432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17445..18479)
                     /locus_tag="PLO58_00620"
     CDS             complement(17445..18479)
                     /locus_tag="PLO58_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQO73322.1"
                     /translation="MSTIKDISKKAGVSISTVSLVLNNKGYVKPETYQRVLSVMHELN
                     YKPSRSARKLATRKNGNIGFIIWESHFYEVEMFYSQVFLGMEYAARKSDSYILLTTVK
                     DQFDPKTDLPRFLQCNDVDGVALAGRVPHELVYYLEKERIPFVLIDYSIPGKKYNSVQ
                     IDNYNGAYSAVQHLVELGRKRIGFVGGTFFHSSIKERYRGYKDCIETNKVMTADDISK
                     YTYVEDTETSPAIGEKGISSLLKNDAKLDAVFCCNDPTAMGVIKEIQRRQLKIPEDIA
                     VVGFDDIPNSSYSTPRLTTVHVPKLALGKEAFNLLNKVIEHPLEPPQTRVIATTLVKR
                     ESTGIKETVE"
     gene            complement(18671..>20614)
                     /locus_tag="PLO58_00625"
     CDS             complement(18671..>20614)
                     /locus_tag="PLO58_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO73323.1"
                     /translation="ESDYHGCLNVSSVNPHYLIYHDGTPFYGIGAYYPWGVRNDDDGL
                     GILAASGGNLFGYWNGNYDGAGNGGGIYQIESIQSGIGYYDQRKCARIDEILDWAEQR
                     NLEMMLAIWPHDILDQSTWGYKGWDDNAYKTVCNAANFYTDSLAWVYQQKLYRYMLAR
                     WGYSRSLGIWELVNEIDGTDGWSRGNKTGALEWVQKVHDYLKENDYFKRPTTISKSGS
                     SANYWPEGYQICDLPNVHLYEKGWSAPYSSDPIRSSYWTYRNVARQLWTGFEKPGIFG
                     EAGAEPSNMYAANIEKGSSKYVEIYHNALWSAWASGLAATPIWWEMNDRELMTDEVFA
                     QMKAFARVASDIDYADLNLISSNASADTADAFMMTADSLGFGWFRSYNNSAISGRFTR
                     ISGLKTGTYQAIWYDTWTGDSINVCNCVSAGNMIYTEIPEMPTPCKDIALRLTKTEDG
                     TQPARLKIFIDKPIITSFPVTNYLIICYVSDAENRLCADSFQISLTLEGPGIISNPQV
                     TTNNGVAVFNYLPEQGKSGEVIFTASTEGLASVSLFNNVLSEIEVEKNASIPTEFALK
                     ANYPNPFNASTVIEFSLPKTTNIKIDVYNLTGQLVETLVRGRQSAGNYKIFWHPANLS
                     SGIYLYQITCPEFTATRKCMILK"
     assembly_gap    20615..20714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20715..21616)
                     /locus_tag="PLO58_00630"
     CDS             complement(<20715..21616)
                     /locus_tag="PLO58_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73324.1"
                     /translation="MKKSILIIMLSTISLFGQIPGFTDDFEDGSLDTLWNDSLMTLWH
                     SDWYPGIFNLTESEGVLEVAYDRKSTDSDWPAFYFTPPEVINVAHNPKIVIEIKSDIT
                     INFAIKPIYTNGKDNYQSTTVPGSNTWQTYTFTLSNYAGGYCNQVVFHFDGGTKVDKQ
                     GTVCFDNFIIAGFSISIQGLNAKFDPINQYVDLVWNCDNEAAVDHYNVYRDTLKGFSC
                     TTENLIGEPEGKNWRDTQIGIGTYYYKVAAVDTMGKQHAVSMEMACRTYTIGAIPVVE
                     IADVNSSTVGKYDKFEITLKLTEAS"
     gene            complement(21671..22798)
                     /locus_tag="PLO58_00635"
     CDS             complement(21671..22798)
                     /locus_tag="PLO58_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HQO73325.1"
                     /translation="MPNQACPSQNGIYQRYQHPVLTAKHIPINWRFDLNYATNPFLME
                     RLGVNAVFNCGAIKFNDKYCLVARVEGLDRKSFFAVAESPNGVDHFKFWEYPIIMPET
                     EIPDTNVYDMRLTRHADGWIYGVFCTERKDPNVPEYDTSTAVAQAGIARTHDLKKWER
                     LPDLQTKSRQQRNVVLHPEFFEGKYAFYTRPQDDFILAGTGGGIAWGLADNIEKAIID
                     EEIIIDSRVYHTINEVKNGEGPAPLKTAEGWIHLAHGVRNTAAGLRYVLYMFLTDLNE
                     PWRIIHKPGGYFLAPEGEERIGDVSNVVFSNGWIADPNGRIFIYYGSSDTRTHVVTTT
                     IERLLDYVKNTPEDGLRSAKCVEQRVALINHNRNIIDNFKK"
     gene            complement(22878..24098)
                     /locus_tag="PLO58_00640"
     CDS             complement(22878..24098)
                     /locus_tag="PLO58_00640"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HQO73326.1"
                     /translation="MKNDAIANWRQQVSRNLYENIIPFWMTYSIDRRNGGFYGRITND
                     LQVDKTAPRSVVLIARILWTFSALFRFDKDSRHLEMANRAYNYLTKYFSDSKYGGVYW
                     LVAPGGEVLEDKKKIYGQAFAIYGLSEYYAASGEKAALEMAHQIFQLIEKYCHDAING
                     GYYEAANRDWSETQDLRLSAVDLNEKKSMNSHLHLLEALTNLLRVSSDSLVRARLQEL
                     VECHLRYILNPVSNHLYLFFDEKWNPRLNGVSFGHDIEASWLITESAEVLGDDKIQAR
                     VKSAVHKIVAAVIAEGFNQHNALPVANDITGSLSVNNFEWWQQAEALVGLINAYQLYG
                     DSSYLDRACRLWDFIDKYFVDWQRGEWYYEISAEGIPNPAKPKVSEWKCCYHNTRACL
                     EVLKRTAQLINRRE"
     gene            complement(24139..25962)
                     /locus_tag="PLO58_00645"
     CDS             complement(24139..25962)
                     /locus_tag="PLO58_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HQO73327.1"
                     /translation="MRLKLIDIVIILLYLSFTVVIGLVLKKRAQENKTAYLLGGNKLP
                     WYMLGISNASGMFDISGTMWLVTLMFVYGLKSIWIPWLWPVFNQIFLMVYLSAWLRRS
                     NVTTGAEWIGTRFGSGRDATLSHNIVVVFALLSCLGFLAYGFIGLGKFMEIFIPWEVV
                     SNYVPFSVPDKFVPHLYGIVFTAFSVFYTILGGMVSIVWADVIQYCIMGVSAIVIGII
                     AMRALSIHPLIVPEGWMSPFFGWELNMDWSGIIAGVNDKIASDGYSLFMIFFMMMLFK
                     GILVSAAGPAPNYDMQKILATRSPKEAGMMSGFVSVILNPIRYIMITGFAVLGIIYYD
                     RLDLIVGGRLDFEQILPSAISEFVPIGFTGLMLAGLLAAFIGTFAGTLNAAQAYIVND
                     IYLKYMRPKATNRQVSSVTYISGIGIVVISIIFGMFALDINSILQWIVSALWGSYVVS
                     NVLKWYWWRFNGYGYFWGMVAGIIPALIFPQIFDTLDLYYFPLILLISLAGALIGTYR
                     HPPTDEAVLKKFYKTVRPWGFWKPIHEKVVAEDPNFVNRSSWKRDLLNVLIGIIWQTT
                     LVALPIYFVLKKFGNAGICLLIIIVTSIFLKKNWYDKMEDD"
     gene            complement(26016..26846)
                     /locus_tag="PLO58_00650"
     CDS             complement(26016..26846)
                     /locus_tag="PLO58_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HQO73328.1"
                     /translation="MKKLFRFYIVLIIIVSLAFSQEKNKVDLSTGANFIATKTYAPEE
                     DARILKLFEGLRVADVSDGMDMVGLPGVGLVDPAIHADWLDLEDLSHQIRGIAITARY
                     VPTQRPDRPQAGEKFQEWEGNFYNVYSPETFAELIRPGSVIVLDDVEEKDIGTIGSYN
                     ILAWHKSGAVGVVTDACARDTDEIAKEKVPLYLRKKGRGIRPGRNELESVNRPVVIGG
                     VLVCPGDVVVADGDGVIVVPRNVAEQVARYAREILVKDKASRKDLYKSLGRPLDKTVE
                     "
     gene            complement(26865..28166)
                     /locus_tag="PLO58_00655"
     CDS             complement(26865..28166)
                     /locus_tag="PLO58_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HQO73329.1"
                     /translation="MNSDKEMLLKELEFALKNEFLDVWYPAALDTVYGGFLSDFTFDW
                     RPEGPQNKMIVTQSRQVWTASQAAMFYQDDQYRKIAEHGFNFLRDKMWDKTYGGFYQL
                     RDRQGNLIPYGDGLHKVAYGNAFAIYALATYYEAFGDTSALDLARQTFAWLEKHNHDS
                     QYKGYVDATNLDGSWIMWHPTGKTNRDITETVLKDYNSSIHLLEAFTQLYKVWPDSLV
                     RERLLEMLTLVRDTFVNKKGYLNMYFTRDWQPLSVYDFSAEPQEADYFFDHVSFGHDV
                     ETAYLLLEAEHILGKKSYSVTLPVARALVDHALENGWDQDNGGLYYQGYYFDGSDTIT
                     IFDDTKEWWVQAEALNAFLLMAKLFPQDQKYYDAFRKQWEYIQRYLIDHQHGGWYSKG
                     LDKSPERAKGPKGYDWKVNYHETRALINCIQMLKDEHELLK"
     gene            complement(28292..29314)
                     /locus_tag="PLO58_00660"
     CDS             complement(28292..29314)
                     /locus_tag="PLO58_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HQO73330.1"
                     /translation="MMRIPYIFGITVLLIFMVSCQSGSKAVDAFEQNEKLGRGVNILG
                     YDPIWDSFEKARFKEHHFKLIREAGFNTVRINLHPFRHMDPTTFELREHWWNVLDWAV
                     ENALANGLMIILDMHEYYAMADDPVARKDMWLAFWKQVAERYQNSSENVLFELLNEPN
                     GKLNSELWNDFLLEGLAVVRNSNPHRTVIIGPAQYNQINQLDSLILPEKDRNIIVTIH
                     YYSPMAFTHQGASWAGRTDSLGVQWLGTAEEKQAIMDDFAKAQNWSKKHNRPLFLGEF
                     GVYDKADMESRTKYLSFVTRTVENLGWSWAYWQFDSDFILYDIDNEHWIEPVLKALIP
                     KTAGVN"
     gene            complement(29351..31129)
                     /locus_tag="PLO58_00665"
     CDS             complement(29351..31129)
                     /locus_tag="PLO58_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73331.1"
                     /translation="MNPLYKIMLVFIVFLAIVQPSRAKENYKNFKLAIYCPARDVQRM
                     ADLNWLEKNFNLLDKYLDIDKVYLETYRGQTLVDKETILKAKKFFKERGIQTSGGITY
                     VWNDDQQFLSFCITNPDQRKRMQEVAEYTASMFDEIMLDDFWFLNTKTESDIQAKGDQ
                     SWTDFRLEQAAEASQKLIIDPAKKVNPKVKIIIKYPNWYDHFQYCGFNLEKQPTQFDL
                     IYTGTETRDPVYTHQHLQAYESYAIMRYFENIAPGRNGGGWVDPFNRRTLDRYAEQIN
                     MTLFAKARELTLFCFGALLEAIPQEDGSFRPLSPVAPVAGYNLDKIDCFLGKLGQPFG
                     IKTYKPYHSSGEDFLPNYIGMLGIPMEITPDFPANDEIIFLTEQASFDAKIVEKIKNQ
                     LIKGKDVVITSGLYRALQGKGIEDIVELEYTDKKALVHRFYDWQNVFIAENDILIPQI
                     RYATNDSWELITALDSGNGYPIFHQASYANGKLYVLTIPDNFSELYELPREVLTIIKR
                     VMMKNFFVYTDSPSSVSLMVYDNNTFIIYSFKDHNTDVNIVLDNKFSKVTDLLANREL
                     TGYKENNKLVIKTAIGPHSYRVFRAE"
     gene            complement(31345..32448)
                     /locus_tag="PLO58_00670"
     CDS             complement(31345..32448)
                     /locus_tag="PLO58_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HQO73332.1"
                     /translation="MNRREFNKMLLCAVGSLALPKIDLFGSKLEQAPPLHFPRYRGFN
                     LLAKFSNWEPRSKFDEEDFEIMAELGFDFARIPMSYWCWSSKDDWYRIDEDVLKDIDE
                     VIEFGKQYNIHINLNLHRIPGYCINGRDQEPMDLYEDTPERMQKALDAAIFHWKMFAK
                     RYKGIPNSQLSFDLINEPPHTKEETRHVEVVTALVNGIREEDPHRLIVADGKDVGRTP
                     IFGIADLGVVQSTRGYDPMSVSHYKANWVPKESYQTFKKPTWPLKGDDGTIWDKAMLK
                     SQLIDPWMPLVAKGVPVHVGEWGCYNQTPHDVALRWMRDLLSLWQEVGWGYALWNLKG
                     DFGILDSKRKDVKYEKYKGHDLDRKMLELLKEF"
     gene            complement(32550..35153)
                     /locus_tag="PLO58_00675"
     CDS             complement(32550..35153)
                     /locus_tag="PLO58_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO73333.1"
                     /translation="MKSKNLVVWFTMLAVVLIMSAGQILAQTGDTLVVNWDDGAGNIV
                     QDALYNAVIGDTVAGGARANLNRVYKLKAGGYYWNYKTISNDFPLRLVGEVPTGDEHP
                     AVIQMVTDAESGSTPGKMISCASDLTLKNLYIIGSDELGSSGYYQPIEVNAVAKRFII
                     DNCVFERSNFAIVAFAGGKDNDLFITNCVYRNLQEDVPTQQWCGRGLSVWTDIDTVVI
                     ENNTFFNVNMCAIQIENGAANYLRFNHNTLVNIGRAFTSTTNVWWREAYFTNLLFVNT
                     WWHGEGYCDYSVQYQPGRDPRAYTTGIFTINDMPSRYGTNKGRRILFSNTAAYLDPYF
                     TEHYGDTIRFQPYVNAITDSFFTTFSPANGGQMVIKDTTWLSTKPAFSSDPNTPAQLE
                     AMWNHITASRGYQYYGTGVQAKPYYYALPTDPVTGDTLWAEPSWPLPENFTYSNDLTT
                     QSTDGLPLGDLRWFPAAKATFEANKDSYVNAIEWMPGERIIIKPIWEEQAEAGTVSGT
                     AAVEPFTGEAWYTLQGGTSVEWTFNSTYAGHIDMKMKARADGQNIGFDFILNGKNIVD
                     AARKWGQFVVWTGSGDPSTFWTGKSTTEFYEAFYAFADLHSFGSLDEIGVVAGENTLK
                     LQYSWNPISFQWIELYEAGTTNLIARLVPSAAVNNGATPGGIGDWVPQGFNSVALGTN
                     GTVTFSLPNLDAGRYQVRVFYQNAGAASTGKVLVDGAQATTFSFASQPAGTGLDVITD
                     LFDLTAGTHTLAISGSGAKVDWIQLLNESRISGIKKVTVLDGFSLSQNYPNPFNPTTM
                     IRFELGKPSNVRLTVYNLLGQRVATLVNGHMSQGAHIIQFDASHLASGIYFYAIQAGE
                     FKEVRRMLLLK"
     gene            complement(35352..38291)
                     /locus_tag="PLO58_00680"
     CDS             complement(35352..38291)
                     /locus_tag="PLO58_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO73334.1"
                     /translation="MKRYLKPAFSALAVMLFINLLNAGVIRGRVVDSKTGDPLIGANL
                     ILAETAGIGAASDFKGYYRIDNVPAGEYTLKVTFIGYQPVEIKVSLKADETIVRDIEM
                     VYGGALKGEEVVVTAQAKGQMSAINQQLASNVITNIVDESRIQELPDVNAAESIGRLP
                     GVSIQRSGGEANKVEVRGLNPKYSLITVNGVELPATGSEDRSVNLSLISSNMLDGIVL
                     KKTNTPDMDADVLGGTIDLRLKEAPEGFKFNLSAQGGYNQLQKYYGNYNFTASVSNRF
                     FDNRLGIIVNANTDNYDRSADKLRDNWRNYNEMLATGELFMIEEIVNRKRTGASLLLD
                     YNLPKGKITANGFFNQLNSDGLIRNSQVNTMELGYATNRHYYRVEEEKSKTNVFTSAI
                     GINQDFGWFRYDAGVSYSGTNTDSPDRRVTEFVQEQSGLLPGYPKFVDPKALVPYFNV
                     DTINTNMASLYSYSKKITENTSSAKLNLEIPFRFGNILSGSFKTGGKLRWIDRSNDEN
                     QHGYSGLQYGSGQQNPVFLHVDKVYPEWGIMDNVEEYQLLNVLPFLKDYSRPDFLNGD
                     FPLGLTFDEKKLHKMSDALMAAPDSLNLWLPYSVGTFGYDYDGFERYQAAYFMGEFNF
                     GSYLTLIPGVRYEKDYSKYHGQRYMVNQSGQTVEQPPSEFVRLTKERENEFWLPMVNI
                     IAKPTNWLNIRLARTETIARPDFLRYAPISYISSDRKSVNAANYSLRPSQSTNYDVGV
                     SLFNNEIGLLSVTGFHKNIEDLIFYSAAFYRPGVVLDSALDIPQSWLKANPQINAYRN
                     NPDPAKYYGYEIEWQTHFWYLPSVFKGIVLNINYTHIYSEMKLVYDSLVTQQVGSRRV
                     YSLVPAYIKTRMPDQPAHVFNITIGYDLKGFSARLSYLFQTDKLTGIGYDGVIPTTRL
                     SSYTSDYGRWDLTLQQKLFQNIQLFANFNNLNNRYDQSCIGADLTHPSYIEYYGFTMD
                     LGIRVNL"
     gene            complement(38288..39340)
                     /locus_tag="PLO58_00685"
     CDS             complement(38288..39340)
                     /locus_tag="PLO58_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQO73335.1"
                     /translation="MTIKDIARLANCSLSTVSKALNGRQDVSEATRQRIMQIVAEYNF
                     TPSAYGKGLKKQKTENIGVIFCRETRPLSANPFYSRVLEGIEAELVINNYNLVLHLLP
                     EVYRGEYPKMLKEHTVDGIILAGVLDQDFVDLVKESGIPIVFVNPNFEIEGEDQIFID
                     DEHGAFQAIQYVISKGHQRIGFVSGDLNRISFKKRYDGYKKALAYHRIEYDDEIVRTG
                     GLENGYDQVTALMKLKNKPTVIFCTNDLNAIFGYKAIEDLGYRIPQDISIIGFDDIDL
                     AKISNPPLTTVRVYKEEMGSIAVRILLEKINKTEHKPVTTLLPVRIIERDSVKDLNNL
                     KNTVRHIEPKQDVELV"
     gene            complement(39820..>40832)
                     /locus_tag="PLO58_00690"
     CDS             complement(39820..>40832)
                     /locus_tag="PLO58_00690"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HQO73336.1"
                     /translation="GAKMKKYKISNDKGVSAEIISYGAIVVSLTAPNRNGQLEDVILG
                     FDDLASYEKDNTFQGSIVGRYGNRIAGGKFSLDGKVYQLNLNDGPNHLHGGPKGFFKV
                     IWDIEQLDSQSVRLTYVSPDGEEGYPGEVKISVVYTITDDDALRIDYYGTTDRPTILN
                     PTSHCYFNLSGSPKNTILDHELMLNADFFTPIDEFSIPTGEIRPVAGTPLDFREPKPI
                     GRDINAEDEQIKFGKGYDHNWVINGKPGQVRLAATVYESKSGRLMECLTDQAGLQFYS
                     GNFLDGSLIGKGGIRYNYRCALCLEAQAFPDSPNKPHFPSVVLRPGEEYRQTTIYRFS
                     VR"
     assembly_gap    40833..40932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<40933..42311)
                     /locus_tag="PLO58_00695"
     CDS             complement(<40933..42311)
                     /locus_tag="PLO58_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQO73337.1"
                     /translation="MFKLFAGLDVSTQSTKLVIIDLAAKKVVYVNSVNYDTDLPHYNT
                     KNGAIQGLGEGVSESDPRMWIEAVDRVFANAVAAGIDMSQIGCIAVSGQQHGLVALDA
                     QGNLSRPTSKLWNDFSTLEECEILTEKVGGLEQMIAEVGNSQRTGYTAAKIFHMVRHE
                     PEIYHKTATLFLVHNYINYYLTGGVRVMEPGDTSGMALWNPKNGQWSQKVIQAIAPDL
                     MSKLPEVKPSDQPIGYISSELVAKFGFSKDCLIDAGCGDNMYGAVGTGNVEPGIVTVS
                     LGTSGTAYTFMEEPYIDPTGEIAAFCDSTGHYLPLLCVSNLANGYNEFIKLHKMTHQV
                     FDKMLDETPPANNGKLLIPWYVGERTPDLPLAAPIYFGFGLADFTQANLARAIIEGHI
                     LNLYTGFKRMPVDVKEIRLTGGLSRSDTWCQTIADIFEAQTVPVEGEGAALGAALHAA
                     WTWSKAQNK"
     gene            complement(42335..43675)
                     /gene="xylA"
                     /locus_tag="PLO58_00700"
     CDS             complement(42335..43675)
                     /gene="xylA"
                     /locus_tag="PLO58_00700"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HQO73338.1"
                     /translation="MSKQVLIGKREYFKGIGKIPYEGPESKNPLAFKYYDENRVVAGK
                     KMKDHFRFAIAYWHTFCNNGGDPFGAEGTVDFPWNESKDPMQAAKDKLDAAFEFFTKL
                     GTPFYCFHDRDMAPEGKNVVESEKNLKELVKIAKAKQEESGVKLLWGTANVFGHKRYT
                     NGAATNPNFYVIPHVAAQVKAAIDATIALGGENYVFWGGREGYYSLLNTNMKREIEHM
                     AMFLTKARDYGRASGFKGTFLIEPKPMEPTKHQYDFDSATVIGFLNKYGLQNDFKINI
                     ENNHATLAGHTFAHEVQTACDAGLFGSLDINQGDPFNGWDTDEFLHNLYDATELMLIL
                     LQNGGIGHGGMNFDAKNRRSSTDLEDIFIGHIYSMDTLARGLLIANDILEKSDYLKMK
                     KERYSSFDSGDGARFEKGELGLVELAKIAEEIGEPKMISGKQELYESLINQYIR"
     gene            complement(43965..>45111)
                     /locus_tag="PLO58_00705"
     CDS             complement(43965..>45111)
                     /locus_tag="PLO58_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HQO73339.1"
                     /translation="IRKIADNVLKDANFNFIDQESGKRYSSTADAPPGARLVPESRYN
                     DWRYWNGVLNIAMLRLGEVLKEPTYTEFAVRNVAFSFDNYAYFEKRYANENKWEYPFG
                     QFFMMEELDDCGAMGASVIEIMRLDPQERYLKYIDRAATHILTRQSRLEDGTLVRTFP
                     RKYTLWADDLYMANSFLCRMGKVTERSWQTVGIDDATRQVINFHKYLFDQEMGLMYHN
                     WYSDTGSPGVAFWGRANGWAILAQVELLDQLPADYPQRDTLLALFRCHIDGIVPYQDS
                     TGLWHQLLDKPDSFPETSCTAMFTYAIARAVNQGYLESRYKSFALKGWQGILTKIRPD
                     GQVEGICTGTSTSDDLSDYYTRPTPLNDGHGIGTVLMAGTEIIQLLK"
     assembly_gap    45112..45211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<45212..46080)
                     /locus_tag="PLO58_00710"
     CDS             complement(<45212..46080)
                     /locus_tag="PLO58_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQO73340.1"
                     /translation="MTDQQWETLKKVINGEKIEPLPVGFIADSPWLPGWYGIKILDYF
                     SNDELWLKANLKAIQEFPEVMFLPGFWSEFGMCTEPSAFGARCRFPQNEFPHAHPVIK
                     SPEEINALVVPEPETDGLLPFVLNRLKLAQPYIENAGHKIRFAIARGPLNIASFLMGT
                     TEFLMAMLTSPELVETLMEKITAFLKNWLRLQRSTFSTIDGILLLDDIIGFIGEQEFC
                     RFGLPYFKELFSADVSVKFLHNDAQCRVSVPYLPEIGVNLFNMGFDVSLNDIKTWTNN
                     QVTLLGNIPPRDVL"
     gene            complement(46100..47782)
                     /locus_tag="PLO58_00715"
     CDS             complement(46100..47782)
                     /locus_tag="PLO58_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HQO73341.1"
                     /translation="MKKVMITMTLIVASFISAGAFSADQKAFYYNPILAGFYPDPSIC
                     RVGDDYYLVNSTFAYFPGIPVFHSRDLVNWKLIGHVMDRAEQMNLEGLGVSEGIFAPA
                     IRFHDGLFYITCTLVGAGGNYVVTAQNPAGPWSNPVWLPQIDGIDPSLFFDQDGKAYI
                     VYNSVAPNNRPLYEGHRTIRMYQFDYEKLKVISPEIILVNGGTDISKKPIWIEGPHIF
                     RKDNFYYLICAEGGTSWEHSEVVFRSEQVTGPYVPYAKNPILTQRHLDPNRPHPITCT
                     GHADLVMTPAGDWWAVFLGCRPYLPEYEGFFNTGRETFMAPVKWIDGWPVIDPDHAEI
                     QYSYPLPIILEKPIKADIPYSGNFRIRDDFDSETLDLYWVFLRTPHTKWYSLSDRRGS
                     LAIQLRPETCSGKSNPSFIGRRQQHLCGSVSTAMDFVPVSEFEKAGLLVFQNEKHYYF
                     LGKSIEKGEPVVQLYKTDTLLASHKIGEGRNIRLKVVADGAEYSFYYATKPEEWILLK
                     DKVDARFLSTRVAGGFVGCMYAMYATSSGQASSNVAYFDWFEYVGDDEIYHQ"
     gene            complement(47814..>49852)
                     /locus_tag="PLO58_00720"
     CDS             complement(47814..>49852)
                     /locus_tag="PLO58_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HQO73342.1"
                     /translation="QTMGPWRYDINRENIYKVLDETVAARGQYENIYTVGLRGIHDTQ
                     MEGGVDVQEQIALLEQVFKDQREILTKHINKEITEIPQVFIPYKEVQDYYDAGLQVPD
                     DVTIMWSDDNWGNIRRLPRLDDKPRAGGYGIYYHFDYVGDPRNYKWLNTVQISRVWEQ
                     MHLAYRYGVDRIWVVNVGDIKPMEFPINFFMDFAWNPEAWPAERLPEYTRLWAEEQFG
                     AQYAEEIAHILTQYTRFNARRKPELLSPETYSLVNYREAETVVAEYNDLAKKAREIYE
                     ALPTEFHDAFYQLVLHPTEACANLNELYVTVARNRLYAQQKRALTNEIAQRARDLFKK
                     DAEITDRYHNIANGKWNHMMDQTHIGYTYWQQPEENTMPEVIEINVPESAEMGVAIEG
                     STECWPPANSEAILPEFDPYNQSCHYIEIFNRGKKPFKYTIKSGNPCVLISQKKGEIT
                     TEERLWIEIDWKQAPKGKSTIPLIIKGPRKSQVEVKIIIFNPAVPVEELADRFLESNR
                     YVSIEAEHFSNAVNTRGVYWQIIPDLGRTLSSVTPFPVTMSSLIPEGDSPHLEYQVYL
                     FNAGEVKVKAYLAPTQNFPNGPGLRYGISFDDEPVQIINMHAGKTHRDWQNSVSDYIT
                     IMTSVHALKSPGKHTLKFWAVDPGVVLQKLVIETGEVKPSYLGPPESRWFKIAE"
BASE COUNT        14388 a        11466 c         9249 g        14249 t
ORIGIN      
        1 gtgaaaatcc atcggataaa cgttataaat accaaccttc tcatcaagca atttggccat
       61 cgctgtctgg actagtcgat tttgtttttc tttgtcaagt aatccgtaat gcaccgccaa
      121 tagcgaccca atataataat ggctatcgag cgatccatct tcgttataat ttatcaaata
      181 gcccatatca tcattccata gctttgccac tagagctgtt tgcattttat cggccaatgc
      241 catcaattca actacttttt cgggattttt ccccagcact gttgagatat aagcataatc
      301 acgcaaactt ttaatcgcta aaatcgtcat ataactacgc gggccataat tatgtccgat
      361 atcccaccaa tccggacggt acgaccacat cagatcatcc ttctcgagag tcaataagga
      421 gcgctcaaca ctaaccgtca aataaggata aagctgtgta agaaagtcgg aatctccaga
      481 gtggcgcaga tatttagccg ctacctgaat aaaccagaaa ttattccagt tatcggaact
      541 ggcaaactca gtcacatatt taccatcttt ccagtaatag gcatgcggta ttacattgtt
      601 ttcatcagaa tgacgaacaa tgtaatccaa gtcacgccgt acgcgtgcta aatcaaaatt
      661 caccgccgcc aggtcggtaa ccaaagcatc gtgagtaaaa taaaaattat actccgcggg
      721 acagggcatt gggacgatct caccgtctag gtaatgggcg ttcgcggaca tcaccgcctt
      781 agcgtaagcc accgaatgat cggttgccgg tgaaccggtt ttcattagac tactcaggaa
      841 agcctgttca tttacgtatt tttcatactg gtcaatttcc aattgataat ttgcccgaag
      901 atatttgact aaatttttac cttcttctgg ttttgacgaa ccgataatct gaatgatatt
      961 cagagattcg cccggtttta accccttttt ataaataaat tcagcgaaag gtaattgaac
     1021 tacattagca gtcaaacact ctaccggcaa atcgccatca ttagtgataa acacaacggc
     1081 ggaatcagca tcggcatcat aaaaattatt ataaatcgta ttgtcttcaa tttctgaaac
     1141 cgccttgtca atttctctaa aggtatgaca ggtgcggatc gaagttctga gtctggtatc
     1201 gaattgaaaa tcctgagcga taccggaatt attgacaatt tcgatctcga caaccattgc
     1261 cggtttttcg ttacaaaagc gatatgcagc ttttactata tattcatcat ctttataatg
     1321 aaaatcaaca gaatacggtg taaggtcgaa ttccgccggc ttcttcccga tttcgatatc
     1381 tgcagaattg ccgattttta atgtccagtc ggcaacaaag gagctatcac gggtccagta
     1441 atcgcggctc agatcaatgc tattagctac tggatagaaa aaactaatcc gctgaggaat
     1501 catatagcta tgatggaatt cggcgccgac aaaaggcccn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnt gaggcagatc gtatttgatt gtggtgaccg gattgaaagg
     1681 attgggatag ttctgagata gtttataaga agtcggcatt tccttggtat cggaacgaat
     1741 agcgctcggc tcagtatata gtttactgat ttcacctatt gataaagcgt agtcataaat
     1801 ccgtatatca tcaatcacac ccttgaaatt gtattgactt actgcaggca gacactgccc
     1861 aatcattaaa tcgatattgg tggttaaaat atcaccggaa aaggtagtat ggttgcttaa
     1921 ctcgccgtca acatataaat caaaatctat tccgttatat agtccgacaa tatgatacca
     1981 ttgattgaca ttaatcgttt tatcggaatc gagatcttta acaccagtcg tcgttttaat
     2041 cgtccagcgg attttttttt gcgggataat ggacactttc cagcgatttt cccaattgcc
     2101 gtgagagatt gggtaagatt cgcggctggt atagagttca gcggcattta tccagaagct
     2161 aactgtaatc gctttagtga agttcagact aacgctattc ggtacccgga tattgtcgga
     2221 cgtcccattg aagtaatagg cgctgttcaa tttaccaaag cggtcggcac tcaaactagc
     2281 gccgttgaca gtaccgtggt tattaaaacc gctcagatcg tcggcattgc ctgcaaaagc
     2341 aaagtacgcc agcggagcgc caatttcatg accggatgta tcaccgacaa cgataagaat
     2401 actatctgaa gctaccccgc caaacaggtc acttacagta catttaatta aaaaataacc
     2461 ggacgagtcc ggcgcttgcc agactgcctg cgcactgtca ctctccagta tcccgcttgc
     2521 ggctgaccaa ttgaatatca gcgaatcgcc atcgggatcg ctggcactgc aattaatcaa
     2581 ggttgtatca gtcggcgcaa tccgacgcgc ggaagcggtc agactggaaa tgattggtac
     2641 ctggttttga accgtataaa ctaatatttg gatgctatcc gctaccggtg tattcacctc
     2701 atcgctgact ctgcaaataa ttgttaaagt agtattaagc tctggcattg tcaattctat
     2761 gatagaagaa tcacagccga tttctgaatc atttatttgc catgaatagt gcaattcgtc
     2821 ggcgtcacga tctactgcgg tgcagtaaat cttataggtt ctcccggctt tgagcgcagt
     2881 tgaatcgcta gcaatggctt taatacgcgg cttataatta gtcacagttt gcccggaatg
     2941 ataatcaaca acaatatcat tcaccagcat tttttcctcg acatagcgga ctgttccgcc
     3001 ggaaggtacc aggacaacca aaaccgcgcc atcggcggga atgttaagat ttgtttcacc
     3061 ggtaattccg gttacaataa aagttttact aactgcatcg tatagatcaa ctgcctgtgt
     3121 tccgacattg aacgaaatcg atttaacctc gctgtaagga ttataaaaaa gataactcgg
     3181 ataggcatct ttctggtagt aatcggtcct gcggagatcg agttttaaaa ttcctgtgac
     3241 attggtcgta tcaattatac caccaagaat accgacatgc gatgaaccgt acaatgcgaa
     3301 attggtggct ccccagccgc cggagatggc atcaccggtc gcaaagggac tgtatcccca
     3361 gtttggatga atctcacgca tagattcgtg cgctatatag ccttctggat cgtattcctt
     3421 cgcccaggtc tcgccatcct gaaggctgtc cggcaagaaa ggtgagtaga atagacggga
     3481 ggcattggct agatttaaga cccacttgcc gattgcccga gcgaagcgac tgtcgtaacg
     3541 caccatcggc accagcgcac ccgccatttc aaaaccgttc atagcaaagg catagccttc
     3601 attcttcgct tcaccgacta acccatagca gtcgtaatca ccccatttac cgagcgtcac
     3661 gccccaattg cggagattat tctcaggatc aaaacaccag ttcaacaatt tctcgatgtt
     3721 ataatttgtg ccctgttcgg cattcatccg ggcggcgaca tagacaccgt atggcaattg
     3781 cagctcataa gaaggattag cagaaaaagc attgagaaac tcgaggcacc actcagcccc
     3841 gatgcggtac ttggcttcgc cggtcttagt gtaagcattg tacagtagcc aggctatcgc
     3901 accagcggct tccggttcgg gtacaccaga ggtgaacggc ttcattgtcg agagatacca
     3961 gccacgataa ttcatattcg gtttttgcca gggcgccgca ctgccgccca tagcaacgac
     4021 tgctctcagc cattgatctg ctactgaagt aaattgataa tcaaaatcgc cggtaccggg
     4081 ataaaggtca tataactgat aaaaaaaaac attaggcatc acggcatacc accaatcatc
     4141 gccactttga gccgtcggac tgtttaaata gacattttct tctggccgcc ggttaaacca
     4201 ctcttcacag cccagtaccc aattatatcc attttggtcg ctcttgtcaa ttccaactag
     4261 tgttgctcct ataactgctg ggagaacatt aattgcttcc gcattcgatg gataggttgt
     4321 tccaacaacc gtatgcaaac caaaactgga atgattggga taattaacgg tattattatt
     4381 gagccagacc aacggtaagt actgtccggt acggtttaga tcgaagacta acctatcata
     4441 accacgagag acttctttcc agtcacgcat aaaatatggt gttggcaaat taggcatttg
     4501 ctcgacccgg ttaatattaa tctgttgagc tttgatagaa gtaaacatat ttagtataag
     4561 aaataaaata attaatggtt ttctagacat tgtactgtac tcacttttaa atgacactga
     4621 gtattatcct ggtaaaaatc aggatacatt ttttgcatta ctttaaaaat ccaaccgcaa
     4681 tgtgtatcgg ctaacatttt ttagacgccc aaaatctgca aaggcataat cgaatttgat
     4741 ataggactgg ccaaataatt tataacgcaa tcctacacca aatgttagcc cttcctcggt
     4801 attagtttga ccgattgcct ggtaacctgc tctaatgaat agcatatcat aaattgctag
     4861 ttcagttccg gtattgatac tttcgctgtt atttatagga tggaaagcat ccgcggcaat
     4921 cgtcattttg atatattttg tactcaacaa atcataagcg agaccaaact gcatatttag
     4981 cggtagcggc cattcatcag tcattaattt tcccataatg cgatcattat tgccatcaat
     5041 actttcatcg gcatcataat aaataataag gtcactgcca tctaaagaca tagaagtacc
     5101 aaaattagta atcgtagcac caattcgtaa accactaaaa ctcgtcttat aaagtgttcc
     5161 gatatcaaag gctacaccat gagatctttc atgccagatg cgctcctcga tatattttcc
     5221 atttagtcca atggaaaagc gatcagtcaa cgcataggaa tatgataagc caaaagcaat
     5281 actgctggcg tcaaacattt caccggttcc ttccggataa tctacggtac gcacagccat
     5341 tttgggaacc gtcatagcat ctaaaaaaaa tcccgctgtt ccccagcgac caaatggaat
     5401 aacagctcca atataattga aactgatatc agccaaccat tcgactttct caaacaccgc
     5461 ttctggactt gttatacgac tgattccggc tggattccaa tatagcgccg aaacatcatt
     5521 agcgacacca acgaaagccc cgcccattcc aatggcacgc gatccaactc cgacttctaa
     5581 aaaaggtgcg gcagtcgtac ccacattagt caatcgtttt gagctgccca tactaagaga
     5641 gtttattaat aaaatgaata tcaatacctt tttgtacatt gatttattca taacatcctc
     5701 atttattggg tatgaaaaac aacaaagtca attttgcgcg ccagtccttc atcagttgtt
     5761 ttaacagcca ttaagttgct actataaatt tcgatatttt cagctcttcg taaattgtct
     5821 tcataattgg caatgtattt ttcgagttgt tcctgtctgc ctgttgtgtc ctgattctgg
     5881 gcataaaact cacgacctag atatgccagc attttacgca tcagaagata ttcactccgg
     5941 ctccgttctt ctgcaattac tgagggctta cgatctaacc tctccttcat cccttgttca
     6001 gatagaaccc aatcgcgaac atacattccc actgcatttt ttaagctttc acggacagtc
     6061 attttacttt tataagaaat tttcaccgga ttgactttat agttgatcaa aatatcggct
     6121 acagtcatct ccccagattt aaatgttgca atcggtttat taagaaaatc ccgactggat
     6181 tcgcctatag tatttatttc gcgattagtg atgaattgct caagggttac cacctcagtt
     6241 ggtcggttac gccaaacttc ctcagtcaaa cgattaagcg cttcagcttt tattataaca
     6301 ttttggggcg tcattatttt ctgaacatat tcagtagcta acttctgctc tttcctttgt
     6361 cgaattatac cattgaggct ttgacggttg gcttgaaaat cactttcccg cagtagaact
     6421 tccttttcga attcaactac ctgaaataca tgatagcgct ggccgtcgaa aaatgggtcg
     6481 gaatactgcc ttaatggcaa attatacagt atatttgcaa cttcctgcgg aatatcgcgc
     6541 cagctaattt tatccactat tccataatcg gtcaattcaa cctgaggggc accagtaaat
     6601 atcggagtat gttcaaattg cacaattccc aatgacactt gcctggcaag atcaaactgc
     6661 tttgtactga aatggcgcac gtataacttt gttttcgaat attcaaatgc ctgacgttct
     6721 tcttgttccg aaacctcaac taatcggcga atatgtttca aatatagttc acgattaaca
     6781 gccagccttt tataaaaatc aaccgtcctt tgtaggagtg tatcatcgtc taatcgcaac
     6841 ctctctgcct ggcgggcgag gacaattcta tcaattaatt gatccaaaac tgcttggcgg
     6901 gcttgtttac tggtgaggct ggttattgaa cggggtgcca tctcataggc aaaagcaaat
     6961 tcatcagctg ttatggttct atctgctact ttggcaacaa tattctcttc tgttttttgc
     7021 gcacaaccat taaatagata tatatctatc aataagctta ccaataaaaa atgttttatt
     7081 aatccagaag ttttaatcat ttaatcaatg caaattttcc gattttagtt ccaatgccgg
     7141 gagcttctac atgataaatg tagatcccgt acgaaatttc attatcttct ttagaaagaa
     7201 tatcccacgg ttccattccg ttatctgcgg tactgttatg tacgattgtg tcaatcagat
     7261 gaccgcgcac attataaatc cgaattgtac attgtctggg tagatgataa aaatggaggc
     7321 gtctttcacc tcgtccgaac ttataagtgt tcgctggttc ccaggaggcg gtgacaacat
     7381 agggatttgg cacaacacag atattgtcca gttcagcttt ggctagtacg tcgctggaat
     7441 cggcaccgtg caacgaaaac cgaaagatat caccttctct aaacggcttt ttcattttta
     7501 taaataattt ttcgcccggt tcaggtggat caattgtcgt ccagatggta tcggcatagg
     7561 ggacaccatt aacccaactg gtatcaattt cgaacaatgg gctaagtcgg atcatccaag
     7621 aagtatacct ttttagaata aaactatcat cgggtataat tagagcaata tcatcaccgc
     7681 tggtataggt taaactacta tcattgtcaa taattgcata atccatttcc accgaatcgg
     7741 taagattata aacttggaca tttgaaggac aggaataata actccagagc ttcaatgaat
     7801 tcttgtaaaa gtcactatga aaaataacca gaaaatctgc cggcaattcc aaattcattc
     7861 ggtaaccagc tacattctca ttatctggat ttaacctgac tttaaggata taatccgaag
     7921 agccttcaat aaaaccgctc ttatcttcat ccaaagttat gttagtatcg ttataaacgg
     7981 taacagtata cccgtcgagt acgggacttt cggctcccga tgacttgaca taagtagaat
     8041 ccagaaccag tgtatcggct acaacattcc gaacctgata ccagggatga gcttcagtat
     8101 ggtatttgga atcttccagg aaaaggatct cgtaggttgt tagtccagaa ggtattttat
     8161 gtttttcaac cagatcaata gctattgaac cagttccggg tccgacatgc gctatttctc
     8221 catcaagact cggaggaatg tagccggcgc tggctggacg gggtatcgcc atcccgcaat
     8281 taacatctaa aaaagtcact tgccccaaag aattagtgcc gataatacag gaacactctg
     8341 aaggtggcaa tccttctact ccggattcac tataatgata caaaccataa tcataggata
     8401 caacggcata atagtaagtt tgaccgttct tgacatctct atcagtatac gtatgtcgca
     8461 gacctgtgtc ttcaccaaga ttaaactgga cgccataaat tgcaacctga tgcgggccct
     8521 ttttaccgtc ttgcaaatca aattgagcaa ttggtttgcg ataagtagta ttcccataag
     8581 catccgtaat gactttgctc tctagaaaat caggtgtagt gccgcgataa atcaggtaac
     8641 cttcgaagtc atactcctgt aaaaagggat catatgaaga ttcggcaata tcatcccaaa
     8701 ctaatgtaac ttcaccatct cccggataga ctttcaaatt aggcttgagg ggtggcttag
     8761 caaaagaata atccgaatta tatatttgct gaacggtctt tttattctta aagagatcat
     8821 cgcggtcttc accaaataat aaagccattg aatagcgttc aatactgttt cgcatcaagg
     8881 gaaagggacc agatgaaaaa aacatgctga gattattcgg ctgaagtaat tgatctaccg
     8941 gtttgggaga tttttcaaaa acatgataat tttcttcatc attggtaagc tcataggtgt
     9001 gagtcgggaa aatttcaaat ccagtcaagc cgatttgatc agattcatcc gggtcagtat
     9061 cattataatt tggttctttg gggtctgtcg gtatgccgtc accttcacct tcatccggcc
     9121 caaaatactg aggatcataa gggccaacac catccttacc tttatcatca tttagcggtt
     9181 catccggatc ccaaacacca ttaccattga catccgtaaa aggtgtccag tcacagtctt
     9241 catcagctga ccaatgagct cttggttcat aattataaac ctcaataaac tttgcccgct
     9301 gggcatcagt aaaaccatag ggatagtcac taatgtaaac tccaggtcca tcactaaccc
     9361 gtgactcgtc tatcattccg tcattatcat tgtctttacc atcatttgaa atccccggac
     9421 tttctaagaa agcatagcca gcgtaaccaa tcggatacca gtttccaggt gtacccaccg
     9481 ctcctggagg accgtaggca aaggctatat cgagatcagt attatattca ccgatgtttt
     9541 gaacatcgcc accaactcca ccgattcccc agtcaatata ttgagcaaaa tagcatttaa
     9601 ggtaatcatg ggtggattcg ttcttaatat catatagcca gaatatgcaa tcttcagcca
     9661 gaacctgact ccattgaaaa ccgcgagctt tgacgactag tccgatacca ccgcgcgtgg
     9721 tatcccgctc atcacagtaa aaattccact ttacattagg ttctttatcc tgatcatcgt
     9781 caaatacgaa aatggtttca agatcagcat tcaacactcc tctcccaaaa taaccattcc
     9841 agtaaccatt ccagctcata tcttgatcgg gccagtaatc cggccaagta tttggattat
     9901 tactcatagc tggagacgta ttatattgct catccatatt aaaccagaag ggttgcggct
     9961 caaatcccca gggaataccg tcaggcgaaa tatcaacgaa ttcgcgaaac tgagtttcca
    10021 tcggataaat tgtttcacca tttctatttt cagttttaac agaaacaatt aaagtaatat
    10081 catcaagata atgacgctcg gcactcggcc agcaaccgga aggctcatcc ggatagtctc
    10141 ctacctcaca ataattccaa aatagggttg aaacgagatt tccattcatc aaaccccgac
    10201 gccgatacat tcgatgtccc cgtaagctag agggagtttg cgctaaagca gtcactccga
    10261 gaaatagcat tataattaag agagatttga aaaataattt cttattcata tttgaacact
    10321 tcactttttt tgatcaaaac taatagagca accaataatt acgttgcgtg gttcttcata
    10381 ccagttcggc tggatattcc actcctcttg ggttttataa ccgcgataat tttcgaaaat
    10441 catgctgtaa ttataatcgg ctgttccgct ggaaggataa acttcgagat tattcaggcg
    10501 gtcaaataaa tttgtaatcc ttataaatgg gctaatcatc aaattcttaa aaggaatatc
    10561 tttagatagg ttcaaattga aagtgattgt cattggtttg cgttcaccat ttagaattcg
    10621 attattgtaa tatggagatt cgcgggtata gggtgtgccg ctaccgattt tcccaatgag
    10681 gcaaatatga aatcccttct gggtagttgt agtagttaaa tttagtgaat gggtttgatc
    10741 ccagttaagc ggctgacgtt ttttttcaga ttcaatgggg ggatcggatc cctgattcct
    10801 atattcatct aacggatcag aagcattgcc ggtcgctgac tggtaggtat agtcaacaga
    10861 aaagcctatt cctccgggtt tttctaacat ttcgattgcc agagtgattc ctttaacatt
    10921 ggcataatcg cgattaatgt agcgccaaaa ttttccacca tagatatcgg tcatagtaag
    10981 ctgtccgact aaatcccgaa tatctttatt atattcagtc aattcgattg ttaagttatc
    11041 tgtaatggct tgtttcaaac cgacctcgta gctgactgtt ttctcaggtc ttaactcggc
    11101 attacccatc acattgaagc cattgctggg aggttcactt ccttctaatt gaacaagcga
    11161 aacctcaaaa tcaggattag cataaagata ctcataattc ggtatctgaa aaaaatgccc
    11221 gtaagaaaaa tgtattacgc cttgatctga aatcgggtaa gcaatcccaa tccgaggact
    11281 aaattgatat gaagatttag ctttacgttt gggagctcct actgtattgt aaaattgagt
    11341 agggacgaca ccatctggtt caaaataatc atacctcaag ccaacattaa ttatcatatc
    11401 gccaaattca attttatctt gtaggtaggc tgaaatctca tatggcttat gtagatatga
    11461 attattgtaa caagaagaat ctgaaggtat ttgaatatta tcattttcat caaaataaat
    11521 ctggtattcg tgaagagata aatgaacctt tttataactt actccagcac cgatattttg
    11581 atgctcggta atttgactac gaagatcagc cttaatgatc tcagtacgat tatccctaaa
    11641 atgatgccac attcgcatgc cacctgtaga aaaaccattg gaattagtgg catttaggta
    11701 ttcaattggt acataacgtg gatcgtattt atctttataa acatattgtc cataggtagt
    11761 aaacaagttt gaataattcg cggttaagaa cgtattagga ttgaacatat gagtatactg
    11821 caatgagttt tgatagctca tattttcatg gcgataattg ccgtccggat tatatttaaa
    11881 taatcggtca taactacggt atttcgattt ttcatagaaa ccggtataat ttattttatc
    11941 catacgagaa agcttaaaac tgagttttcc ctggagagtt tgcatatttg atgaattcat
    12001 cgagacaaaa gagctatcgc cagttgactg gataatctgc ttgtttggat catcagaaaa
    12061 ataacaaaaa tccgaagggc ggaaaatccg ttgaccatat aaccagccgt catcggtatt
    12121 cgtccgaccc aggagagaaa acgataagtt tttccccaat aaaggaatag ggccactaag
    12181 gttaaattga tagttagtat tatccaatgg atttatatca tcaatattca taaaaatatg
    12241 agtatgtgaa cttaagtgat ctccacagta aaatgtgatt ttgcctgaat aatattcttg
    12301 cccttcttta gtcacaatat tgacgatacc agacatagcc tgaccgtatt cagcattaaa
    12361 agtaccactg atgacttcaa cttcttgaat catatcactt tcaacatcga ataaatgctc
    12421 accagcataa gcatcattca tcggaactcc atcgataata tacatcactt ctcctgctct
    12481 tcctcctcta aagtgcccct gaactacccc ggcctgaagc tcaacaattt cactcaaatt
    12541 ttgaacttgc aattgatcaa tttgttcaga gctaacccga aacgaagtcg aagtaacatc
    12601 ttttctgata agcggccttt gggcaatcac tactacttct tcaccttcca agattgttga
    12661 aaccatttca aaatcaagag gtgtggtcaa atcaacagaa acctggacat ttttttgaat
    12721 gacagtttga tacccaatca tactagcttt tacggaataa ttacctggta acacattaag
    12781 tataacatag ttaccttcaa tatccgttgc ggcgccttgg gaagtacctt caagaacaat
    12841 attaacaccg actagcggct caccactaat tttatccgta acccgtcctt tgatctttcc
    12901 cgtggtacct gcttccagaa gcgaaatgta taaaattaca agtataatta ttaagattgt
    12961 ggttcgcttc ataataatcc ttacctgatt tataggttaa aaacctccga aggcgggaaa
    13021 tagccttcgg aggtttttat ctttgcttat cgcatgagaa ccatttttct tgtaaccctc
    13081 tggttattaa ttgtcaatac ggcaatatat ataccagtcg gtacattctg gccgaaatca
    13141 ttccgagcat cccaagtcag ggtaaaagtc ccagcatttt gaactgtgtc ggtcagatta
    13201 cgaacctttt gccccagtat attatatacc gtcaaattaa ctttaccaga ttgcgggagt
    13261 gaataaacga tcctcgtttc cgggttaaat ggattggggt aattatccat aattgcatac
    13321 ttgacaggga tatcaccgcc aaatccttta tcagtaatga taccagtacc gggagcaacc
    13381 ttcatttttt cgacggtgaa gtcatcgaac caggctttac cagtcacttt attattaaat
    13441 cgagcacgaa ccgaaatccc tgtcgcaggc tcatctcccg ccggtgcctg ctcagctacc
    13501 acataatgag tccaaccagt acttgcctct cgctgatcta tatatacaaa cttatcacca
    13561 cccggcaact tatcccaagc cgtctcaata tcggcatctc catgaaagaa ataacataaa
    13621 cttatcctct caggtatacc ttccttcgta atataagtcg gctcaaattc agacgaatcc
    13681 agtacattct ctgtcttaac ccatacgcct atcttatacc attcacccgc ctctaccgga
    13741 taaggcttgg aataatatac caactcattc aactgcgaac aggtatccgg ctgttccatt
    13801 actaccgaat aactccccgt atgagcttca gcatcagtta ccaccgcata actaccattt
    13861 tcagaatacc aatccatcca gccattaaca tcttcagcac cataattgaa tatacccatc
    13921 gtccaggtgt ccgtcgtatt acagtctata ttatcaaaat aaactgtccc agtcgcctct
    13981 ttacccatta ccaatttaac caccactgac gccggcgctt ccgtcaatat tgccacccct
    14041 gtcaatttgg tccagtcctt atccgcaaca ctctgatctg cccaaactgt ctcggtcgtc
    14101 aactcaccgc cggatgcgtt cttgaactcg tatactatcc caaccttctc agcatcactt
    14161 gccggattcg tattcacacc gaccgtcttg acatatccgg tcaaggcata tgtcccggcc
    14221 gtcgcattgt tccagtacaa tgtcgcattg ttatcagaca cccatccaac tacatccgaa
    14281 ctggccgcac tcttcgtaat ctcaaaagaa tgaaaaccag caaaatgctc cgtagatgaa
    14341 atatctacgg cttccccaaa tgtcccattc aaaggactcc aaaacgccgg gctctgatcc
    14401 tcaaaactac cattccctat caaattatcg ccatcaatga taattttttc gacggtgaag
    14461 tcatcgaacc aggctttacc agtcacttta ttattaaatc gagcacgaac cgaaatccct
    14521 gtcgcaggct catctcccgc cggtgcctgc tcagctacca cataatgagt ccaaccagta
    14581 cttgcctctc gctgatctat atatacaaac ttatcaccac ccggcaactt atcccaagcc
    14641 gtctcaatat cggcatctcc atgaaagaaa taacataaac ttatcctctc aggtatacct
    14701 tccttcgtaa tataagtcgg ctcaaattca gacgaatcca gtacattctc tgtcttaacc
    14761 catacgccta tcttatacca ttcacccgcc tctaccggat aaggcttgga ataatatacc
    14821 aactcattca actgcgaaca ggtatccggc tgttccatta ctaccgaata actccccgta
    14881 tgagcttcag catcagttac caccgcataa ctaccatttt cagaatacca atccatccag
    14941 ccattaacat cttcagcacc ataattgaat atacccatcg tccaggtgtc cgtcgtatta
    15001 cagtctatat tatcaaaata aactgtccca gtcgcctctt tacccattac caatttaacc
    15061 accactgacg ccggcgcttc cgtcaatatt gccacccctg tcaatttggt ccagtcctta
    15121 tccgcaacac tctgatctgc ccaaactgtc tcggtcgtca actcaccgcc ggatgcgttc
    15181 ttgaactcgt atactatccc aaccttctca gcatcacttg ccggattcgt attcacaccg
    15241 accgtcttga catatccggt caaggcatat gtcccggccg tcgcattgtt ccagtacaat
    15301 gtcgcattgt tatcagacac ccatccaact acatccgaac tggccgcact cttcgtaatc
    15361 tcaaaagaat gaaaaccagc aaaatgctcc gtagatgaaa tatctacggc ttccccaaat
    15421 gtcccattca aaggactcca aaacgccggg ctctgatcct caaaactacc attccctatc
    15481 aaattatcgc caaatgcgaa gtttaatagg atcaagccga caaccatggt taacaaacaa
    15541 gtctttttca ttttttcctc ctatgtttgt tgtttgtttt tatgtgattt taactcattt
    15601 tgtattttta aataagccac tcccttgcgg ggcaacgtca aataataaag tccttcctct
    15661 tctatcttga gatcagattc atcagaagaa attgaaagag gcgggtggac tatataaaga
    15721 gctccgtttt gtctctgcca agaatgatta agtactatca cccgataatc tacctgattc
    15781 tcccagaccc ctaattctag tccatcacct tcaatcgtta tttccgctcg tttaccgtga
    15841 agccggttaa tagatttgta tatacggcaa agaattgccc taaggtgatc atcattgatt
    15901 actaaagcta acacttcaag tggatatgat gtgaaataaa cttttccttt tccaatcgca
    15961 ttttcaagca gaaaaggcat tccatattca tcagtcataa tgactctagc tgtcgtactt
    16021 attattggac aataacttaa agccggttga ttatttaaaa gagatatagt aaatatttca
    16081 tttttagcga attttcccca ctcattttct accttaacag ttaaatcagt tttaactcta
    16141 aagtcaggaa ctccaaactt gcaatcggta actaccccag ccaatttatc ccagtcagtc
    16201 acccaactat cattggcaaa actaaaatat attaccccac catctcggac ataattttcc
    16261 agggcaattc tagtttgttt tgtcataaat cttaaacccg gtagaaatag caccggtaaa
    16321 tcttcaggtt ttagatcgag ggtatgcgaa tagcaaccgt cattttcaag ttcctgagcc
    16381 ggttctacta ccattcgaac atcaagatta gcccgtttca gcagtgagaa acattccaga
    16441 aaaaaatcgt aatgattttc accctctact ttaaacatat atgggtagtt gtagtaataa
    16501 cttgatggta tgaaaagtcc ggaattatac tcgactttat tatattttcc tgttatcaga
    16561 tcgctggaaa tcttttccaa tttctggaat tcataagccg ctggtttcag actttgatca
    16621 gttctgataa tcccaaaacg ttcttcaaaa agatggtgag aatatggtct tttatcttta
    16681 aaatcaaaat catttaggca ccagtttaaa acaccctgtg ctccatttat taaagcacta
    16741 taaaatacat tccgataata attagcttga tttatttccg aacacaaagt agtagaagtg
    16801 ccaaattctt ctacaagaac cggcttaccc caaacttttg ccaactggat acggaacgca
    16861 gtcgtataag aatggtgcca cggactcata ccgcgtggat agaaatgtaa tccaacgaaa
    16921 tcctgatata aattcaattt ccttagatga aatccagttt gatcggcgat gatttctggt
    16981 gcccaggcac catctccaat actgataggt cgttgcgaat caattgcccg aatcgttgcg
    17041 ataatttctt caacccacgc agttacatca tcaggctcct gtttaccaac gaaattaggt
    17101 aattcattag atagtagcca ggcttgaata tacttgaaat tgtttatcgc cttaattacc
    17161 gtggtgatat aaaacttaat tgcgtcaaca acacgcctat ctttaagaag gtcactccct
    17221 tttaaccatg gcatgcccca atcctcaccc gacatatgtc caacaataaa agtcggtaaa
    17281 gtatataatt cattttcttc gcatatactt agaaaatatt gtaaccgttc cannnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncattattc ttctctactc tactgtttct
    17461 ttaatgcctg tactttctcg cttcacaagg gtcgttgcaa tcactcgcgt ctgcggcggt
    17521 tccaggggat gttcaataac tttattgagt aaattaaaag cctccttacc taatgctaat
    17581 ttgggaacat gtacggttgt taatcttggt gtagagtaag aggaattagg aatatcatcg
    17641 aaaccaacaa cagcaatgtc ttccgggatt ttaagttgcc ttctttgaat ttctttgata
    17701 acacccatag cagttgggtc attacaacaa aataccgcat ccagtttagc atcgtttttt
    17761 aatagactgc taattccctt ttctccaatg gcaggcgaag tttcagtgtc ttcgacgtag
    17821 gtatacttag agatatcatc agcagtcatt accttattgg tttcaatgca atctttataa
    17881 ccacgatagc gctctttaat ggatgaatga aaaaaagtac ctccgacgaa accaatcctt
    17941 tttcgcccta attcgactaa atgctgaaca gctgaataag caccattgta attatcaatc
    18001 tggacactat tatacttttt acctggaatt gaataatcaa ttaaaacaaa cggaatcctt
    18061 tctttttcta aataataaac caattcgtgc ggtacccgac cggctaaggc aacaccatcc
    18121 acatcattgc actgtaaaaa acgaggtaga tcagttttag gatcaaattg atctttgacg
    18181 gtcgttaaaa gtatatagct gtcactttta cgtgcggcat attccatacc tagaaaaacc
    18241 tggctgtaaa acatttcaac ttcgtaaaaa tgactttccc aaatgataaa tccgatatta
    18301 ccatttttgc gggtagctag cttacgcgcc gaccgcgagg gtttgtaatt aagttcatgc
    18361 atcacactaa gaacccgctg gtaggtttct ggtttaacat aacctttgtt attcaagaca
    18421 agcgaaacag tcgagatgga aaccccagct ttttttgaga tgtctttaat tgtactcata
    18481 ttttctcata aataatatcg aaacgtttta atataacacc ttagaaatta aaagtcaatt
    18541 aaaattatta gacggaaata tttttaataa atatagattt agtaaacttt aagtatatct
    18601 atataaataa atagattata taaacaatta aaaaaataga aaaatataaa ttagtttaag
    18661 tcgctttctc ctatttcagg atcatgcact tcctagtagc cgtgaattcc ggacaggtaa
    18721 tttggtacag atagatacct gacgacagat tagccggatg ccagaatatt ttgtaatttc
    18781 cagccgattg ccgtcctcgg acaagtgtct cgactaattg tcctgttaga ttatagacat
    18841 caatctttat gttcgtagtt ttcggcaggg aaaattcgat cacggtgctg gcgttaaagg
    18901 gattaggata gttagctttc agagcaaatt ctgtcggaat ggaagcattt ttttcgactt
    18961 cgatctcgct caacacatta ttgaatagag aaacgctggc taatccttcg gttgaggcag
    19021 tgaagataac ctctccggac ttaccttgtt ccggaagata gttaaaaaca gcgacgccat
    19081 tattggtagt cacttgggga ttgctaataa ttccaggtcc ttcgagagtt agcgaaattt
    19141 gaaaagaatc agcgcaaagg cgattctccg catcactgac atagcatatt atcagataat
    19201 tggttacagg gaacgaggtt ataatcggtt tatcaataaa aattttcaag cgcgccggtt
    19261 gagtcccatc ttcggttttc gtcaaccgta aggcaatatc tttgcacgga gtcggcatct
    19321 caggaatttc agtatagatc atattaccag ccgatacaca attacagaca ttgatactat
    19381 cgccggtcca ggtatcatac cagatagctt gatatgttcc ggtttttaag ccactaatcc
    19441 gtgtaaaacg accggaaatc gcactattat tatatgatcg aaaccagccg aacccgaggg
    19501 aatccgccgt catcataaaa gcatccgcgg tatcggcact cgcattactt gaaatcaggt
    19561 tcaagtcagc atagtcaatg tcagaagcaa ctctcgcgaa ggctttcatc tgggcaaaaa
    19621 cctcgtcagt cattagctct cgatcattca tttcccacca gatgggcgtg gccgccaaac
    19681 cactcgccca ggctgaccag agcgcattat gataaatttc gacatattta ctggaaccct
    19741 tttcaatatt tgcggcatac atattactcg gttccgcacc ggcttcaccg aaaattccag
    19801 gtttctcgaa accggtccat aattgacggg cgacattgcg atatgtccaa taactacttc
    19861 ggattggatc gctggaataa ggcgcactcc atcctttttc gtagaggtga acattgggca
    19921 aatcacaaat ttgataacct tcaggccagt aattcgcaga acttccgctc ttggagatag
    19981 tcgtaggtcg tttaaaataa tcattttcct tcagataatc gtggactttc tgaacccatt
    20041 cgagcgctcc ggttttatta ccacgtgacc agccgtcagt gccgtcaatc tcattcacca
    20101 attcccagat accgagactg cgcgagtaac cccagcgagc aagcatatag cgatacaatt
    20161 tctgctgata tacccaggcg agcgaatcgg tataaaaatt agcggcgtta cagacagttt
    20221 tataggcatt atcatcccaa cccttataac cccaggtgct ttgatcgaga atatcgtgcg
    20281 gccagatggc aagcatcatt tctaaattac gctgctccgc ccaatctaaa atttcatcaa
    20341 ttcgagcgca tttacgttga tcataataac cgatgccact ttgtattgat tcgatttgat
    20401 aaattccacc gccgttaccg gctccatcgt aattgccgtt ccagtaaccg aacaggttgc
    20461 cacctgatgc ggccaaaatt cctagcccat cgtcgtcatt gcgcacgccc cagggatagt
    20521 aagcgccaat tccatagaac ggtgtgccat cgtgataaat cagataatgc ggattaaccg
    20581 aagatacatt taaacagccg tgataatcgg attcnnnnnn nnnnnnnnnn nnnnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20701 nnnnnnnnnn nnnngaagcc tcggtcagtt ttagagtaat ttcgaattta tcatactttc
    20761 cgactgtact cgaattgaca tcggcgattt cgaccaccgg aattgctccg atcgtatagg
    20821 ttcggcaagc catttccatt gaaaccgcgt gttgtttgcc catcgtatca accgccgcaa
    20881 ctttatagta ataagtaccg atacctattt gcgtatcgcg ccagtttttc ccttccggtt
    20941 cgcctattaa attttcagtc gtacaggaaa agcctttgag agtatcgcga tacacattat
    21001 aatgatcaac agccgcttca ttatcgcagt tccagactaa atcaacatac tggttgatcg
    21061 gatcgaattt ggcatttagc ccctggatac taattgaaaa tccagcaatt ataaaattat
    21121 caaaacagac tgttccctgt ttatcaactt tcgtcccgcc gtcaaaatgg aacacgacct
    21181 gattgcaata accaccggca taattactca gcgtaaaagt ataagtttgc cacgtattcg
    21241 aacccggaac ggtagttgac tggtaattat ctttgccgtt cgtgtaaata ggtttaatcg
    21301 caaagttaat cgtgatatcg gatttgatct cgataacgat tttcggatta tgagcgacgt
    21361 tgatgacttc cggcggcgta aaataaaatg ccggccagtc gctatcagtt gatttccggt
    21421 cataagccac ctcgagaact ccctcgctct ctgtcagatt gaaaataccc ggataccagt
    21481 cgctatgcca gagagtcatt agagaatcat tccacaatgt atccagcgaa ccgtcttcaa
    21541 aatcatccgt aaaaccgggg atctgtccaa atagcgatat cgttgatagc attataatca
    21601 aaatcgattt cttcatgata ataccctcca agattccccg attaaaactt aatgttcata
    21661 gaacagacaa tcatttttta aagttatcaa tgatattccg attgtgattg attagtgcca
    21721 ctctttgttc gacacatttt gccgatctaa gtccgtcttc cggagtattt ttcacataat
    21781 ccagaagcct ttcaatcgtg gtcgtcacaa cgtgagttcg ggtatccgat gatccgtaat
    21841 aaatgaatat gcgcccgttc ggatcggcga tccatccgtt actaaaaact acattggaga
    21901 catctccgat gcgctcctct ccttccggag ccagaaaata accacctggt ttatggatta
    21961 tccgccaggg ttcattcaaa tcggtcagaa acatatacaa gacgtaacgc aaaccggccg
    22021 cggtattgcg aacaccatgc gccagatgta tccaaccttc ggcggttttg agcggcgccg
    22081 gtccctcgcc atttttcact tcgttgatcg tatgataaac tcgactgtca ataatgattt
    22141 cttcgtcaat aattgctttc tcgatattat cagccagtcc ccaggcaatc ccgccgcctg
    22201 ttcccgctaa aataaaatca tcctgtggtc gtgtgtaaaa agcatatttg ccttcgaaaa
    22261 attccggatg tagaaccaca tttctctgct gtctcgactt cgtttgcaaa tccggaagcc
    22321 gttcccattt tttcaaatca tgagtgcgag caattccggc ttgcgccacc gccgtagaag
    22381 tatcatactc cggaacgttg ggatccttgc gctccgtaca gaacacacca taaatccagc
    22441 catcggcatg acgggtcagc cgcatatcat agacattcgt gtccggaatt tcggtttcgg
    22501 gcataattat cggatattcc cagaatttaa aatggtcaac gccgttcgga ctctcggcaa
    22561 ctgcgaaaaa cgacttgcgg tcgagtccct ccacccgcgc taccagacaa tatttgtcgt
    22621 tgaacttgat tgcgccgcag ttaaaaacgg cattgactcc taaacgctcc atcagaaatg
    22681 gattagttgc ataattcaga tcgaatcgcc agttaatcgg aatatgttta gcggttagga
    22741 ccggatgctg atacctttga taaatcccat tttgcgaagg acacgcctga ttaggcaaat
    22801 tgagccaagc gtcttgatcg gttagtaatt tatttaaact gttcttgaat tcatttttac
    22861 tgggtttctt caatttgtta ttctcttcga ttaatgagtt gagcagtgcg ttttaggact
    22921 tccaagcaag cacgggtatt atggtaacaa catttccatt cgctgacctt aggcttagcg
    22981 ggattcggaa tcccttcggc ggaaatttca taataccatt cgccccgttg ccaatccaca
    23041 aaatatttat caatgaaatc ccagaggcgg caggctctat ccaaatagga tgaatcacca
    23101 tagagctggt aggcattaat cagaccgaca agcgcttccg cttgttgcca ccactcaaag
    23161 ttattgactg ataaactccc ggttatatca ttagccacag gtagagcatt atgctgatta
    23221 aagccttcgg caattaccgc cgctacaatt ttatggacgg ctgatttaac ccgcgcctga
    23281 attttatcat caccaaggac ttcggcggat tctgtgatta accagctggc ttcgatatca
    23341 tgcccaaatg aaacgccatt caagcggggg ttccattttt catcgaaaaa taaatataaa
    23401 tgattactca ccggattgag aatgtatctc aggtggcatt ctaccaattc ctgcagacga
    23461 gcccgcacta aactatcgga actgacccgc aggagattag tcaaagcttc cagtaggtga
    23521 aggtgagaat tcattgattt tttttcgttt aaatcaactg cgcttagccg cagatcttga
    23581 gtctccgacc agtcacgatt agcggcttca taataacctc cattaatcgc atcatgacaa
    23641 tatttttcaa tcaactgaaa aatctggtgc gccatttcaa gagccgcttt ttcaccggaa
    23701 gccgcataat attcactgag accataaatg gcaaaagcct gtccataaat ttttttctta
    23761 tcttcaagga cttcgccacc aggcgccact aaccagtaaa ctccaccata tttactatcg
    23821 ctgaaatatt tagtcagata attgtatgcc cggttagcca tttcaagatg ccggctatcc
    23881 ttatcaaatc taaaaagcgc cgagaaagtc cataatatcc gagcaatcag caccacactg
    23941 cgcggtgcgg ttttatccac ctgtaaatcg ttggtaatcc ggccataaaa tccaccattc
    24001 cgacgatcaa ttgaatatgt catccagaac ggaattatat tttcgtataa attgcgacta
    24061 acctgctgac gccaatttgc aattgcatca tttttcatat tctatctcga tttgccaatt
    24121 ataaagattt cttcaaaatt aatcatcttc cattttatcg taccaatttt tcttcaaaaa
    24181 gatggaggtt actataatta tcaataaaca aatcccggca ttaccaaact ttttcagcac
    24241 aaaataaatt ggcaaagcca ctaaggtcgt ctgccagata attccgatca gtacattcag
    24301 taaatcccgc ttccaggaac ttcgattgac aaaattggga tcttcagcaa ccactttctc
    24361 atgaatcggt ttccagaaac cccagggacg caccgtttta taaaattttt tcaagaccgc
    24421 ttcatccgtt ggtggatggc gataagttcc gattagcgct cccgccagcg aaatcaataa
    24481 aatcaatgga aaataataca aatccagcgt gtcgaaaatc tgaggaaaaa ttaacgccgg
    24541 aataatccca gctaccattc cccagaaata accatagccg ttgaatcgcc accagtacca
    24601 cttcaaaaca ttggaaacga cataactccc ccataaagcc gaaacaatcc attgcaaaat
    24661 actattgatg tccagagcaa acattccaaa aatgatacta atgacgacga taccaattcc
    24721 cgatatataa gttaccgatg acacctggcg gttggtggct ttaggacgca tatatttcag
    24781 gtaaatatca ttaacaatgt aagcctgcgc ggcgttcagc gttccagcaa aagtaccaat
    24841 aaatgccgcc aatagtcccg ccagcatcag accagtaaaa ccaatcggaa caaattcact
    24901 gatcgccgaa ggtaaaattt gctcgaaatc cagacgtcca ccgacaatta aatccaaacg
    24961 atcgtaataa ataatgccca acacggcaaa tccagtaatc attatataac gaatcggatt
    25021 cagaatcacc gaaacaaaac cgctcatcat tccagcttct ttgggcgagc gagtcgccaa
    25081 aattttctgc atatcgtaat tcggcgccgg gccagccgcg ctgactaaaa tacctttgaa
    25141 cagcatcatc ataaagaaaa tcataaaaag cgagtagccg tcgctggcga tcttatcatt
    25201 gactccggca ataatgccac tccaatccat attcaattcc caaccgaaaa agggactcat
    25261 ccagccttcc ggtactatca ggggatggat cgaaagcgcc cgcatagcaa ttattccaat
    25321 tacgattgca gagactccca taatacaata ctgaatcaca tctgcccaga cgatactgac
    25381 cattccaccc agaatggtat aaaaaacact gaacgccgta aaaacaatcc cgtacaaatg
    25441 ggggacaaat ttgtctggaa cgctaaaagg cacataattg gatactactt cccacgggat
    25501 aaaaatttcc ataaattttc ccaaaccgat aaagccgtag gctaaaaatc cgagacaact
    25561 cagtaaagcg aaaactacta cgatattatg ggaaagggtc gcatctcggc cggaaccaaa
    25621 acgcgttcca atccattctg cgcctgtcgt gacatttgag cgccttagcc aggctgacaa
    25681 atataccatt aaaaatatct gattaaaaac cggccagagc cagggaatcc agattgattt
    25741 aagcccataa acgaacatca gtgtcaccag ccacattgtc ccggaaatat caaacatacc
    25801 ggatgcattc gatattccaa gcatatacca gggcaattta ttgccgccta ataaatacgc
    25861 ggttttattt tcctgtgccc gctttttaag aaccaggccg atgacaacgg taaaggataa
    25921 gtatagcaga attataacga tatcaattaa ttttaatcgc ataaaaattc ccgatttaca
    25981 ttatactatt tcacttactg gtaaattttg gcattttatt cgacagtttt atccagaggc
    26041 cgaccaagac ttttatataa atcctttcga ctagctttat ctttcaccaa aatttcccgc
    26101 gcatatctgg caacttgctc agcaacattg cgagggacta cgatcacacc gtcaccatcc
    26161 gccaccacta cgtcacccgg acaaaccaaa actccgccaa tgacaaccgg acgattaacc
    26221 gattccagct cattacgccc tggtctgatg ccgcgaccct tttttcttaa atataaaggc
    26281 actttctcct tggcaatttc atcagtatca cgcgcacagg catcggtgac aactccgacc
    26341 gccccacttt tatgccaagc taagatattg taagagccta tggtgccaat atccttttcc
    26401 tcaacatcgt cgagcacaat taccgagccg ggtcgaatca actcggcaaa agtttcaggc
    26461 gaataaacat tataaaaatt cccttcccat tcctgaaact tttcaccggc ttgcggtcgg
    26521 tcaggacgtt gggtaggaac atagcgcgcc gtgatggcaa tgccgcgaat ttgatgcgat
    26581 aggtcttcca gatcaagcca atcagcatga atcgccggat caaccagtcc gacgcccggc
    26641 aagcctacca tatccattcc atccgagaca tcagccacgc gcaatccttc gaagagtttc
    26701 aggatacggg cgtcttcttc cggcgcgtaa gttttcgttg cgataaaatt tgccccagtt
    26761 gacaaatcta ctttattttt ttcttgagaa aaagccaagg atacaataat tatcaaaaca
    26821 atataaaatc gaaataattt cttcataatt aatccatcct taagttactt taatagttca
    26881 tgttcatctt ttaacatctg gatacagttt attaaagcgc gggtttcatg ataattgact
    26941 ttccagtcgt atcctttagg acctttggcg cgctcgggac ttttatccag accttttgaa
    27001 taccagccgc cgtgctggtg atcaataagg tagcgttgga tgtattccca ctgtttccgg
    27061 aaggcatcgt agtatttctg atcttgcggg aaaagtttag ccatcaataa aaatgcattt
    27121 aaggcttcgg cttgaaccca ccattccttg gtatcatcaa aaatcgtaat cgtatccgaa
    27181 ccgtcgaagt agtagccctg ataatacaat ccgccattgt cttgatccca gccgttctcg
    27241 agagcatggt caaccagcgc tcgggcaact ggtaaagtca cagaatagga ttttttaccg
    27301 agaatatgtt ccgcttctaa aagcaaatag gcagtctcga cgtcatgacc gaaagaaaca
    27361 tgatcaaaaa aatagtcggc ttcctgcggc tcggcggaaa agtcatacac cgatagtggt
    27421 tgccagtctc tcgtaaaata catattcaga taaccttttt tattgacaaa agtgtccctg
    27481 accagagtca gcatttccag taggcgttcg cgcacgagac tatccggcca tactttgtag
    27541 agctgagtaa atgcctccag caggtgaatt gaagaattat agtccttcaa aaccgtctcg
    27601 gttatatcgc gattggtttt accagtcgga tgccacatta tccaggaacc gtccagattg
    27661 gtggcatcaa catagccttt gtactgcgaa tcatgattat gtttttcgag ccaagcaaaa
    27721 gtttggcgcg ccaaatcaag agcagaagta tcgccaaaag cttcataata agtagccagc
    27781 gcatagattg cgaaagcatt gccataagcc actttatgaa gaccatctcc atagggaatt
    27841 aaattcccct gacgatcccg caattgataa aagccgccat aggtcttatc ccacatttta
    27901 tctcttagaa aattaaatcc atgttcggca attttgcgat attgatcatc ttgataaaac
    27961 attgccgcct gcgaagccgt ccagacttgc cgactctgag ttacaatcat cttattctgc
    28021 ggaccctcag gccgccagtc aaacgtaaag tcactcagaa aaccaccata aactgtatcg
    28081 agagccgctg gataccagac atctaaaaat tcatttttta gagcaaattc aagttctttt
    28141 aataacattt ctttatctga attcatttgt gtagcacatc ctatcaagat tattataatt
    28201 atgcagtaaa ttattaagtg acgatattta ttcaagttat catccaccct tactttatac
    28261 ttctccaaat gattattgcc attgcaatac ctcaattaac gccggcagtt ttgggtatca
    28321 aggctttcaa aaccggctca atccaatgtt cattatcaat atcataaagg ataaaatcac
    28381 tgtcaaactg ccaatatgcc caactccatc ctaaattttc caccgtcctg gtcacaaacg
    28441 aaagatattt agttcgagac tccatatcgg ctttatcata gacaccaaac tcgccgagaa
    28501 acaaggggcg gttgtgtttt tttgaccagt tttgagcttt ggcgaaatcg tccataatgg
    28561 cttgcttctc ctcggctgtg cccaaccatt ggacacccag cgagtcagtt cgaccagccc
    28621 aggacgcacc ttgatgagta aacgccatcg gcgaataata atgaatagtt acgatgatat
    28681 tacgatcctt ttccggcaat attaaactat ctaactgatt gatttggttg tattgtgccg
    28741 gaccaatgat gaccgtccga tggggattgg aattgcgaac caccgctaaa ccttccaata
    28801 gaaaatcatt ccacaattca ctgttaagtt tgccatttgg ttcatttaat aattcaaaga
    28861 gcacattttc ggaactgttt tggtatcgtt cggcaacctg cttccagaat gccagccaca
    28921 tatccttacg agcgactgga tcgtcagcca tagcataata ttcgtgcata tcgagaataa
    28981 tcattaagcc attagccagc gcattttcca ccgcccaatc cagaacattc caccaatgtt
    29041 ctcgtaattc aaaggtcgtc ggatccatat gccgaaaagg atgcaaatta attcggacgg
    29101 tgttgaagcc cgcctcccga atgagtttaa aatggtgctc cttgaaacgc gccttctcga
    29161 acgaatccca gatcggatcg tagcctaaga tatttactcc tctgccaagc ttttcatttt
    29221 gctcaaaggc atccaccgcc tttgatccgg attggcagga aaccataaaa attagtaaaa
    29281 cggtaatgcc aaaaatgtac ggtattctca tcataacatt ctcctattga ttaattttta
    29341 ttgataatca ttattcagct ctgaacaccc gataagaatg cggcccgatt gcagtcttga
    29401 tcactaattt gttgttttct ttatagccgg ttaattctcg attggcaaga agatcagtga
    29461 ctttactgaa tttattatcc aggacaatat ttacatccgt attatggtct ttgaaggaat
    29521 aaatgataaa ggtattgttg tcatagacca tcaggctcac actgctggga ctatcggtgt
    29581 aaacgaagaa atttttcatc attacccttt tgatgattgt caaaacttcg cgaggtaatt
    29641 cgtacaattc gctgaagtta tccggaatag tcagcacata aagtttgccg ttagcgtaac
    29701 tggcttgatg gaagattgga tagccattgc cgctgtcgag cgcggtaatc agttcccagg
    29761 aatcattggt agcatagcga atttgaggaa tcagaatatc attttcggct ataaagacat
    29821 tctgccagtc gtaaaatcta tgcactaacg ctttcttatc agtgtactct aattcgacga
    29881 tatcctcgat gcctttcccc tggagcgcac gataaagtcc ggaagtgatg accacgtctt
    29941 tacctttaat cagttgattc ttgattttct caacaatctt agcatcgaag ctagcctgtt
    30001 cggtcaggaa aataatctca tcattcgccg gaaaatcagg agtaatttcc atcggaattc
    30061 ccagcatacc aatgtaattg ggaaggaaat cttcaccgga ggaatggtaa ggcttatagg
    30121 ttttaatgcc gaacggctga cctaattttc ccagaaaaca gtctatctta tccaggttat
    30181 atccagcgac tggcgcaacc ggactcaaag gtctgaacga gccatcttcc tgaggaattg
    30241 cttccagcaa cgccccaaag caaaataggg tcaattctcg ggctttggca aacaatgtca
    30301 tgttgatttg ttccgcataa cggtcgagcg tgcgacgatt aaaagggtcc acccagccgc
    30361 cgccattccg tccgggcgca atattttcaa aatagcgcat aatggcataa ctctcatagg
    30421 cttgtaaatg ctgatgagta taaaccgggt ctctggtttc cgtgccagta tagatcagat
    30481 caaattgagt cggctgcttt tccagattaa atccacagta ttgaaaatga tcgtaccagt
    30541 tcggatactt gatgattatt tttaccttag gattgacctt cttcgccggg tcaataatca
    30601 gtttttgaga ggcttccgcc gcttgctcca aacgaaaatc cgtccagctt tggtcacctt
    30661 tggcttgaat atcagactcc gttttagtgt ttagaaacca gaaatcatcc agcataattt
    30721 cgtcaaacat actggcggta tattcggcaa cctcttgcat tcttttacgc tgatccggat
    30781 tagtaataca aaacgataaa aattgctgat catcgttcca gacataggta attccaccgg
    30841 aagtctgaat accgcgttct ttgaaaaatt tcttggcttt taaaatggtc tccttgtcaa
    30901 ccaatgtctg gccgcgataa gtctcaagat aaactttatc aatatccagg tatttatcga
    30961 gcaggttgaa gttcttttcc agccaattta aatcagccat tctttgcaca tcgcgcgctg
    31021 gacagtagat cgccaatttg aaattcttgt agttttcctt agctcgcgat ggttgcacaa
    31081 ttgctaaaaa aacgataaaa actaacataa tcttatatag cggattcatc ttttgattct
    31141 ccttttagat ttgactattt cttctaaacc attccttgat ctgctaggac ctttttttga
    31201 gaaatatcca gaatacaatc ggcaggtagg attaattcct catttatttc tgataattat
    31261 aagcaatata atggaccgag tttttatatc attcctgatt ttcaagtttt taattattgg
    31321 aatcttacag cctccatagc gcactcaaaa ttcctttaag agctccagca tcttgcgatc
    31381 gagatcatgt cctttatatt tctcgtattt cacatctttg cgtttactat cgagaatccc
    31441 aaaatcacct tttagattcc aaagagcgta cccccagccg acttcttgcc acagtgataa
    31501 caaatcacgc atccagcgca gagctacatc atgcggcgtt tgattataac agccccactc
    31561 tccgacgtga actggaactc cctttgctac taatggcatc cagggatcaa ttaactgaga
    31621 tttcagcatt gctttatccc agatggtgcc atcatcgcct ttaagcggcc aggttggttt
    31681 cttgaaagtc tgataggatt ctttcggtac ccaattcgct ttataatggc tgacgctcat
    31741 tggatcatag ccgcgcgtac tttgaacaac tcctaaatca gcaatgccaa agatcggcgt
    31801 gcgaccaacg tctttcccat cagctacgat caaccgatgc gggtcttcct cgcgaatccc
    31861 attaaccaaa gccgtgacga cttctacgtg acgggtttct tctttggtat gcggcggttc
    31921 attaattaaa tcaaaactaa gctgagaatt cgggatgcct ttatagcgct tagcaaacat
    31981 tttccagtga aaaatagccg cgtccagtgc cttttgcatt ctttcaggcg tatcttcgta
    32041 gaggtccatc ggttcctgat cgcgtccatt aatgcaatac cccggaatgc gatgcaaatt
    32101 cagattaatg tgaatattgt actgcttccc gaactcgatt acttcgtcta tatcttttaa
    32161 aacatcttca tcaatgcggt accagtcatc tttagaagac cagcaccagt aggacatcgg
    32221 aattcgcgca aaatcgaatc ccaactccgc cataatctcg aaatcttctt cgtcgaattt
    32281 acttctgggt tcccagttac tgaatttagc cagaaggtta aatcctcgat agcgtggaaa
    32341 atgcagtggc ggtgcttgtt caagtttaga accgaagagg tcaattttag gtagtgccag
    32401 cgaacctacc gcgcacagca acattttatt aaattcacgt ctattcataa actttcctaa
    32461 ttcactaact gcatccgaaa aaagagagcc ggcaggtatt ttttcgtcct gccggctact
    32521 ctattcattc caactaatcg gtcgcagtcc tatttaagga gcaacatccg tcttacttct
    32581 ttaaattcac cggcttgaat cgcatagaaa taaatgcctg acgccaaatg actggcatca
    32641 aactgaataa tatgagcacc ctggctcata tgaccattga ccaatgtagc gactctttga
    32701 cctaacaggt tatagactgt caatctgaca ttagaaggtt tgcccaactc aaagcgaatc
    32761 atagtagtcg ggttgaacgg attcggatag ttctgcgaaa gcgagaatcc atccagaaca
    32821 gttaccttct tgatacccga aattctggat tcattgagta attgtatcca gtcaactttt
    32881 gcaccactac cggaaatagc caaggtatgg gtaccagccg tcaaatcaaa aagatcagtt
    32941 atgacgtcta atccggtacc agccggttga ctggcgaaag aaaaggtcgt cgcttgtgca
    33001 ccatcaacta gtactttacc ggttgaggct gctccagcat tctgatagaa cacccgcacc
    33061 tgatagcgac cagcgtctaa attaggtaga ctgaaagtaa ccgttccatt tgttcctaag
    33121 gcgaccgagt taaatccctg aggtacccaa tcgccaatgc caccgggagt agcaccatta
    33181 ttaaccgcgg ctgaaggtac taatcttgca atcaagtttg ttgtcccggc ttcatatagc
    33241 tcaatccact gaaaggaaat tggattccag gaatattgta gcttcagggt gttttcaccg
    33301 gctactacgc caatctcgtc aagactgcca aaactatgca gatcagcaaa agcatagaaa
    33361 gcttcataaa attcagtggt acttttaccg gtccagaagg ttgaaggatc accagaacca
    33421 gtccagacca caaattgacc ccacttacga gcggcatcaa cgatattttt gccattgaga
    33481 ataaagtcaa aaccaatgtt ttgcccatcg gctctggctt tcatcttcat atcaatatgc
    33541 ccggcataag tagaattgaa ggtccattcg acactcgtgc cgccttgtaa ggtgtaccac
    33601 gcctcaccgg taaaaggttc aacagcggca gttccactta cagtcccggc ttcggcttgt
    33661 tcttcccata taggttttat tatgattctt tcacccggca tccattcgat agcatttacg
    33721 taggaatctt tattcgcttc aaaagtcgct ttggcggctg ggaaccagcg caaatcaccc
    33781 agcggcagac catcggtact ttgcgttgtc agatcattgc tataagtgaa attctcaggt
    33841 aaaggccagc ttggttctgc ccaaagggta tcaccagtga ccggatcagt cggcaaagca
    33901 taataataag gcttagcctg gactccggtt ccataatact gataacctct ggaggccgtg
    33961 atgtgattcc acattgcctc taattgagcc ggagtattag gatcactgct gaatgctggt
    34021 tttgtactaa gccaggtcgt atctttaata accatctggc caccgttggc cggactgaat
    34081 gtagtaaaga acgaatccgt gatagcattc acataaggct gaaacctgat cgtatctcca
    34141 taatgttctg taaaatacgg atctagatag gcggctgtat tggaaaatag gatacgacgc
    34201 cctttgttgg tcccatatct cgacggcata tcattaattg taaagatgcc cgtggtatat
    34261 gcccttgggt cacgacctgg ttgatattgt acactataat cacaataacc ttcaccatgc
    34321 caccaggtat tgacaaacag caaattcgtg aagtaggctt ctctccacca gacattggta
    34381 gttgaagtga aagcacgacc aatattaacc aaggtattat gattgaagcg aagataattt
    34441 gcggcaccat tctcgatctg gatagcgcac atattaacat tgaaaaaggt attattttca
    34501 atgactactg tatcaatatc agtccatacc gagagtccac gcccgcacca ctgttgggtt
    34561 ggcacatctt cctgtaaatt gcggtaaaca cagttggtaa taaagaggtc attatccttg
    34621 ccgcctgcga aagcaacaat cgcaaaattt gaacgctcga acacacaatt gtcaataatg
    34681 aaccttttcg caacagcgtt aacttcaatt ggttgataat accctgaaga gcccagttca
    34741 tcagaaccaa tgatataaag attcttcagt gtaagatcac tggcacagga aatcatttta
    34801 cctggtgtac ttccactctc tgcatctgta accatctgaa ttacagccgg atgttcgtca
    34861 cctgtgggta cttctcctac taaacgcaga gggaagtcat tactaatggt tttataattc
    34921 cagtaataac caccggcttt cagtttatag acccgattca gattggcacg agcaccgcca
    34981 gctacggtgt ctcctataac ggcattgtaa agagcatcct ggacaatatt gccagcgccg
    35041 tcatcccaat tcacaaccag cgtatctcca gtctgcgcta aaatctggcc tgccgacata
    35101 atcagcacga ctgccagcat agtaaaccaa actactaagt ttttagactt cattaaataa
    35161 tcctcctttt gattaatttt cttggtttgg caatttaata gcaatttttc tcttaaaatc
    35221 atttttttgg cactttctcc tttcattttt taatgttttt agtttaaaaa acgcacttaa
    35281 ggcgggtgaa tttgaattgc taacatcatt tgatttcccc ctatttatta aaatcaacat
    35341 taaatattcc tctaaagatt gactctaata cctaaatcca tagtaaatcc ataatattcg
    35401 atatagctcg gatgagttaa atctgctccg atacaacttt ggtcgtacct attattcaaa
    35461 ttattaaaat tggcaaacaa ttgaatattt tgaaaaagct tttgctgtaa ggttagatcc
    35521 cagcgcccgt aatcactagt ataactactt aatctcgtcg tgggaattac gccatcatac
    35581 ccaataccgg ttaatttatc agtctggaat aaataagaga ggcgagctga gaatcctttt
    35641 aaatcatatc caatcgtgat attaaaaaca tgggcaggtt gatcaggcat acgagtcttg
    35701 atataagctg gcaccaatga atagactcgc cgtgatccaa cttgttgggt tactagacta
    35761 tcatatacta atttcatctc agaatatatg tgagtataat taatatttaa gacaattccc
    35821 ttaaaaacag atggtaaata ccagaagtga gtctgccatt ctatttcata accgtaatat
    35881 ttggcaggat caggattatt tctataggca tttatctggg ggttggcttt taaccagctt
    35941 tggggaatat ccaaagccga atctaataca actcccggac ggtaaaaagc cgctgaataa
    36001 aatattagat cctcgatgtt tttatgaaaa ccggtaactg aaagtaaacc aatctcattg
    36061 ttaaacaaag aaacacccac atcataatta gttgattggg aaggtcttaa tgaataatta
    36121 gccgcattta ccgattttct atctgaagaa atatatgaga tcggtgcgta tcttagaaaa
    36181 tccgggcgtg caatggtttc ggtccttgct aatcttatat ttaaccaatt cgtaggcttg
    36241 gctattatat ttaccattgg taaccagaat tcattctccc tctcttttgt aagcctaaca
    36301 aattcagaag gcggttgctc cacagtctga ccggattggt taaccatgta gcgttgacca
    36361 tgatattttg aataatcctt ttcataacga actccgggta ttaaagtgag ataactacca
    36421 aaattaaatt cacccataaa ataagcggct tggtaacgtt caaaaccatc atagtcgtaa
    36481 ccgaatgtcc caacggaata gggcagccat aaattcagac tatccggagc cgccatcaaa
    36541 gcatcggaca tcttatgcaa ctttttttca tcaaaagtca atccaagggg aaaatcacca
    36601 ttcaggaaat caggacgaga gtaatctttc aaaaatggca acacatttag caactgatat
    36661 tcctcaacgt tatccataat gccccattct ggatagactt tatcaacatg taaaaatacc
    36721 ggattttgtt ggcctgaccc gtattgtaat ccactatatc cgtgttgatt ttcatcatta
    36781 ctgcgatcaa tccagcgcag ttttccgccg gttttaaaag agccactcaa aatattccca
    36841 aaccgaaagg gtatttcaag gttaagtttg gcggatgatg tgttctctgt aattttctta
    36901 ctatagctat ataaagaagc catattagta ttaattgtat cgacattaaa atatggtacc
    36961 agagccttgg gatcaacgaa cttgggatat ccgggcagta aaccactttg ttcttgtaca
    37021 aattctgtta ctcggcgatc aggactatca gtgttcgtcc ccgagtaact tactccggca
    37081 tcataccgga accatccaaa gtcttgatta attccaattg cactggtaaa aacgtttgtt
    37141 ttactttttt cttcttcaac tctataataa tggcgattag tagcgtaacc gagttccatg
    37201 gtattgacct ggctattacg aattaatcca tcggaattaa gctgattgaa aaaaccatta
    37261 gcggtaattt tcccttttgg tagattatag tctaataata agctcgcccc ggtccgtttt
    37321 cgatttacta tttcctcaat cataaataat tctccggtag caagcatttc attataattc
    37381 cgccaattat ctcgcaattt gtcggcgcta cggtcatagt tatctgtatt tgcatttact
    37441 attattccga gacgattatc gaagaatcta ttgctgacac tggcggtaaa attataattg
    37501 ccgtaatatt tctgaagttg attgtatcca ccttgagctg acaggttaaa cttaaaacct
    37561 tccggcgctt cttttagtct taaatcaatc gtgccgccta aaacatcagc atccatatct
    37621 ggagtattgg tttttttcaa aacgattcca tccagcatat tggacgaaat tagtgaaagg
    37681 ttgacacttc ggtcttcgga accggtggct ggaagttcga caccgttgac cgtaatcaaa
    37741 ctatatttgg gattaagacc tcgaacttca actttattgg cttcgccacc ggaacgctga
    37801 atagaaacac cgggtaaacg cccgattgac tcagccgcat tcacatccgg caattcctgg
    37861 atacggctct catcaacaat attggtaatt acattcgagg ctaactgctg gttgatagcc
    37921 gacatctgtc ctttggcttg cgcggtgacg acaacttctt ctccttttag cgcaccacca
    37981 tagaccatct cgatatcgcg aacgatggtt tcgtctgctt tcagactgac ttttatttcc
    38041 accggttgat aaccaataaa agtcaccttc aaggtatatt cgccagcagg tacattatct
    38101 attctataat atcccttaaa atcggaagcg gctccaatac cagctgtttc agctaaaatc
    38161 agattcgcac caatgagcgg atcgccggtt tttgaatcta ccactctccc tcttattact
    38221 ccggcgttca ataaattaat aaacaacatc accgctaatg cactaaatgc gggctttaaa
    38281 tatctcttca tactaactcc acatcttgct tgggttctat atgtctaacg gtatttttaa
    38341 gattgttaag gtctttcacc gaatcacgct caattattct caccggtaaa agagtagtaa
    38401 caggcttatg ctcagtttta ttaattttct ccaataatat tctaactgcg attgatccca
    38461 tctcttcttt ataaactctc acagttgtca atggtggatt ggatattttt gccagatcaa
    38521 tatcatcaaa tccaattata ctgatatcct ggggaatacg ataccctaag tcttcaatcg
    38581 ctttataacc aaaaattgca ttaaggtcat ttgtgcagaa aatgaccgtc ggtttgttct
    38641 ttaatttcat taaagctgta acctgatcat atccgttttc caagcctcct gtccggacaa
    38701 tttcatcatc atattcaatt ctatggtatg ccaatgcctt tttatatcca tcgtaacgtt
    38761 ttttaaaact aatcctattc aaatcaccgg aaacaaaacc aattctctga tgccctttac
    38821 ttatgacata ctgaatcgct tgaaaagcac catgctcatc atctatgaaa atttgatctt
    38881 ctccttcgat ttcgaaattg ggattaacga aaacaatggg aattccggat tctttaacca
    38941 gatccacaaa atcctgatcg agtacacctg ccagtattat cccatccacg gtatgttctt
    39001 tgagcatttt cgggtattca ccgcgatata cttctggcag taaatgcagc actaaattat
    39061 agttattaat gaccaattca gcttcgatgc cttccagtac acgtgaataa aatggattcg
    39121 ctgatagcgg tcgcgtttcc cgacagaata ttacgccaat attctcggtt ttctgtttct
    39181 tcaagccttt accgtaagca ctgggagtga agttatattc agcaactatt tgcataattc
    39241 gctgacgagt agcctcactc acatcctggc ggccattcaa ggctttcgat acagtcgaga
    39301 gtgaacaatt cgcgagacgc gcaatatcct taattgtcat cgttaaatct gcctaagtca
    39361 caataattaa agtaattaaa aaccggtcat agtaccgcac caatcctgat ttttgtatgc
    39421 tactagcggt cattttcccc taccccgcga tagcctttat cccttttcaa tccatcaatt
    39481 ctcttttacg aaatcgtttt ctgtatccta atttaaatga catttttaaa aatgcaagaa
    39541 aaattatttt aattccattt tatcgtcgag aatttctcta attttgttca ttttcaaatt
    39601 aactaaaaaa tttacactcc agtggttacg aaaatatttt cgataattca tcgaaatatt
    39661 ccgaaatcca taagaatcaa ttacattgtt tcaggaataa ttggataatc tattccgacc
    39721 caattataaa atatgttgcc ttttagcaat cataaaagac aacatttact tgctcgtatc
    39781 ttgagctaat atattagttt attggtactt ataaattttc tatcttaccg aaaaacgata
    39841 gatggtagtc tggcgatatt cttcgcccgg tcgcagaact accgatggaa aatgcggttt
    39901 attaggcgaa tccggaaaag cctgtgcttc cagacagaga gcacagcggt aattgtaacg
    39961 aataccgcct tttccgatca gtgaaccgtc tagaaaatta ccggaataga attgcaaacc
    40021 ggcttgatcg gtcaggcatt ccatcaatct accactcttg gactcataaa cagttgcggc
    40081 gagacgcacc tgcccaggct taccgttaat tacccagtta tgatcataac ctttgccgaa
    40141 ttttatctgc tcatcttcag cgttgatgtc acgaccaatc ggtttgggtt cgcgaaaatc
    40201 cagcggagtg ccagctaccg gtcggatttc accggtagga atagaaaatt catcaatcgg
    40261 cgtaaagaaa tcagcattca acattaattc gtgatctaaa atagtatttt tcggcgatcc
    40321 gcttaaattg aaatagcagt gactggtagg atttagaata gtcggccggt cggtcgttcc
    40381 ataatagtca attcgcagag catcgtcatc ggtaattgta taaacaacag atatttttac
    40441 ttccccggga tagccttctt caccgtccgg actgacataa gtcaaccgaa ccgattggct
    40501 atccaactgt tcgatatccc atattacttt gaaaaaacct ttcggaccgc cgtgtagatg
    40561 atttggtcca tcatttaagt taagctgata gacttttccg tcaagactaa attttccgcc
    40621 agctattcga ttaccatagc gaccaacaat agacccctgg aaagtattat ctttttcgta
    40681 actggctaaa tcatcaaatc ccagaataac gtcttccaat tggccatttc tgttcggagc
    40741 tgtcaaagaa actacgatag ctccataact gataatttct gccgaaactc ctttatcatt
    40801 tgaaatcttg tactttttca ttttcgctcc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40921 nnnnnnnnnn nntccttatt ctgggcttta ctccaggtcc aagccgcatg caaagccgca
    40981 cccagcgccg caccttcacc ttcgactggt actgtctggg cttcgaaaat atcagcaata
    41041 gtctggcacc aggtatcaga ccgcgaaagc ccaccggtca ggcgaatctc tttcacatcc
    41101 actggcatac gcttaaaacc ggtatagagg ttaagaatat ggccttcgat aatagcgcga
    41161 gctaaattag cctgggtaaa atcagccaat ccaaaaccga agtaaattgg cgcagccaac
    41221 ggcaagtccg gagtccgttc gcctacatac cagggaatca gtaatttacc attattggct
    41281 ggcggcgttt catcaagcat tttatcaaaa acctgatgag tcattttatg cagtttaatg
    41341 aattcgttgt acccgttggc taaattcgat acacataata aaggtaaata atggccggtg
    41401 ctatcacaaa aagcggcaat ttctcccgtt ggatcaatgt aaggctcctc cataaatgta
    41461 taagccgttc cgcttgtccc aaggctgacg gtcacaatcc caggttcgac attcccggtt
    41521 ccgactgcgc catacatatt gtctccgcaa ccggcatcaa ttaggcagtc tttcgaaaaa
    41581 ccaaacttcg ccactaattc ggaagaaata tatccaatcg gttgatcgga aggtttaact
    41641 tccggtaatt tggacattaa atcaggagcg atagcttgga tgactttctg cgaccactga
    41701 ccatttttgg ggttccaaag tgccatgccg gaggtgtcac ccggttccat cacgcggaca
    41761 cctccggtca gataataatt aatataatta tgcaccagaa agagagttgc cgttttatgg
    41821 taaatttccg gttcatggcg caccatgtga aaaattttcg ccgcagtata tccagtacgc
    41881 tggctattgc cgacttcggc aatcatttgt tccagcccac cgactttttc cgtcaggatt
    41941 tcacattctt ccagagtcga aaaatcgttc cagagtttac ttgtgggtcg cgataaattg
    42001 ccctgcgcat ccagagcaac cagaccatgc tgttggccgg aaaccgcaat gcaaccgatc
    42061 tgagacatat caattccagc cgcgacggca ttagcaaaaa cgcgatcaac cgcctctatc
    42121 cacattcggg ggtcagattc ggagacacct tctcccagcc cctggatagc gccgttcttc
    42181 gtgttatagt gcggcaggtc ggtatcgtaa ttgaccgaat taacataaac gactttcttg
    42241 gccgcgagat caatgatgac aagtttggta ctttgggtcg aaacatccaa cccggcgaat
    42301 aatttaaaca cggttttact cctatttatg agattcagcg aatatactga ttgatcaggc
    42361 tttcgtacaa ttcttgtttg ccgctgatca ttttgggttc gccaatttct tcggcgattt
    42421 tcgccagttc gacgaggcct aattcacctt tctcgaaacg agcaccgtcg ccagaatcaa
    42481 aactactgta gcgttctttt ttcatcttga gataatccga tttttccaga atatcattgg
    42541 caatcagcaa tccgcgcgcc agagtatcca tcgagtaaat atggccaatg aaaatatctt
    42601 ccaaatcggt cgaactacga cgattcttgg catcaaaatt cataccgccg tggccgattc
    42661 caccattctg caaaagaatt agcattagtt cggtagcatc atacagatta tgtaaaaatt
    42721 catcagtatc ccagccgtta aaaggatcac cttggttaat atccagactt ccgaacaggc
    42781 cggcatcgca agccgtctgg acttcgtgcg caaaagtatg cccagccagg gtagcgtgat
    42841 tattctcgat attgatcttg aagtcattct gaagaccgta tttgttcaga aaaccgatta
    42901 ccgtagccga atcgaaatcg tactgatgct tagttggttc cataggttta ggttcgatca
    42961 aaaatgtgcc tttgaaacca gacgcccggc cataatcgcg agccttggtc agaaacatcg
    43021 ccatatgctc gatttcacgt ttcatattgg tattaagcag gctgtagtaa ccttcgcgac
    43081 cgccccagaa tacatagttt tctccgccga gtgcgatggt tgcatcaatg gcggctttca
    43141 cctgtgcggc gacgtgagga ataacgtaaa aattaggatt agtggcggca ccgttagtat
    43201 agcgtttatg cccaaataca ttagccgtgc cccacaacaa tttgacaccg gactcttctt
    43261 gtttggcttt ggcaattttt accagttctt tgaggttctt ttcagattcg actacattct
    43321 tcccttctgg agccatatca cgatcgtgga agcaataaaa cggtgtgcct aacttagtga
    43381 aaaattcaaa agccgcatcc agtttgtctt tggcggcttg cattggatct ttagactcat
    43441 tccagggaaa atcaaccgta ccttccgctc caaatgggtc tccaccatta ttacagaatg
    43501 tatgccagta agcaatagca aagcgaaaat ggtctttcat tttcttgcca gccacaactc
    43561 tgttctcatc ataatacttg aacgccaaag gattttttga ttcaggacct tcataaggaa
    43621 ttttaccgat tcccttgaaa tattcccgtt ttccaattaa gacctgtttt gacataatta
    43681 atctcctgtt ttttatttac tgttcttacg aatcatagtt ttattaagtg ttaaatgatt
    43741 tgtaatagaa aaaatcataa tattcaattt tgatagtttt atttgcctat attaatattt
    43801 aaagttgggt aggttaaaat ttttttatag atattatcac acacaatctt cataatctaa
    43861 aatttacttg aaccgtttca atattaccaa taaatatttt aaaggtgtta ttcgggatgg
    43921 atttttatct gggacatatc aagcactaaa tctatggttt ggttttactt aagcaattgt
    43981 attatttcag ttccagccat taaaacagtt ccaataccgt gcccatcgtt gagtggcgtt
    44041 ggacgtgtgt aataatcgct taaatcgtcg cttgttgatg taccggtaca aatcccttcg
    44101 acctgtccgt caggacgaat cttggtcaaa atgccttgcc agccttttaa agcaaacgac
    44161 ttgtagcggg attcaaggta gccttgatta acagcccgcg caatagcgta agtaaacatt
    44221 gccgtacaag atgtttccgg aaatgaatcc ggtttatcga gcaattgatg ccacagtccg
    44281 gttgagtcct gataagggac aattccgtca atatggcacc gaaataacgc cagcaaggta
    44341 tctctctgag gatagtcggc tgggagctga tccagtagct caacctgagc caaaatcgcc
    44401 caaccattgg cgcgtcccca gaaagcaact cccggactcc cggtatccga ataccagtta
    44461 tgatacatta agcccatttc ctgatcgaac aagtatttat ggaaattgat cacctgacgg
    44521 gtggcatcat caattccaac agtttgccag gaacgttcag tcactttacc cattcgacac
    44581 agaaaggaat tagccatata taaatcatct gcccagagag tatattttcg cggaaaagtt
    44641 ctcacgagcg tgccgtcttc caaccgactc tgacgggtta aaatatgcgt tgccgctcga
    44701 tcaatatatt tgagataccg ttcctgtgga tcgagacgca ttatctcgat aacgctagct
    44761 cccatcgctc cacaatcatc gagttcctcc atcataaaaa actgaccaaa agggtattcc
    44821 catttatttt cattggcata tcgtttttca aaataggcat aattgtcaaa actaaatgcg
    44881 acatttcgga cagcaaattc ggtataagtc ggctccttca gcacttcgcc cagcctgagc
    44941 atagcgatat tcaaaacgcc gttccagtag cgccaatcgt tgtagcgact ttccggaacc
    45001 agccgggctc caggcggagc atcggcagtt gaagaataac gtttgccgct ttcttgatca
    45061 ataaaattaa agttggcatc cttcaggaca ttatcagcaa ttttacgaat gnnnnnnnnn
    45121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagcacatcg cgcggcggga tattgccaag
    45241 tagcgtcacc tgattattag tccaggtttt gatatcattc aggctgacgt caaatcccat
    45301 attaaataaa ttgacaccta tctctggtaa atatggcacg gaaacccggc attgagcgtc
    45361 attgtgcaaa aatttcaccg acacatcggc actaaataac tccttaaaat agggtaatcc
    45421 aaatcggcaa aattcctgtt ccccgataaa accgataatg tcatccagca gaagaatgcc
    45481 gtctattgta ctgaatgtac ttcgctgaag ccggagccaa tttttcagaa aggcggttat
    45541 tttttccatt aatgtttcga ccaactctgg gctcgtcagc attgccatta aaaattcagt
    45601 cgtgcccatc aaaaaactgg caatgttcaa tggccctcgt gctatggcga accggatttt
    45661 gtgaccggca ttttcgatat acggctgggc taatttgaga cgattcagga cgaatggcaa
    45721 taaaccatcg gtttccggtt caggcacaac cagcgcatta atctcctcgg gtgatttgat
    45781 gaccggatgg gcatgtggaa attcattttg cgggaaccga caacgtgctc caaaagccga
    45841 tggctcggtg cacattccga attccgacca aaaccccggc agaaacataa cttccggaaa
    45901 ttcttgaata gctttgagat tggctttcaa ccacaattcg tcattggaaa aataatcgag
    45961 aattttgata ccataccagc caggcaacca gggactgtca gcaataaatc caaccggcaa
    46021 aggttctatt ttttcgccat tgattacttt tttgagagtt tcccactgtt gatctgtcat
    46081 ttttaatcct cattaagtat tattgatggt aaatttcgtc gtcgccgaca tactcaaacc
    46141 aatcgaaata ggcaacatta gaactggctt ggcctgatga tgttgcatac atagcataca
    46201 tacagcccac aaatccgccc gccactcgtg tgcttagaaa gcgcgcatcc accttgtctt
    46261 tcagcagaat ccattcttct ggcttggtgg cgtagtaaaa agaatattcg gcgccatcgg
    46321 cgacaacctt caaccggata ttacgcccct cgccgatttt gtgtgaagcc agcaatgtgt
    46381 cggttttata taattgtaca accggttcgc ctttctctat cgatttacct aaaaaataat
    46441 agtgcttttc gttctgaaaa accagcaatc cggctttttc aaattccgaa accggaacaa
    46501 aatccattgc cgtactcacc gacccgcata aatgttgctg acgccgcccg ataaaactcg
    46561 gattgctttt gccggagcag gtttctggtc ttaattgaat agccagcgaa cctctacgat
    46621 cacttagact ataccacttg gtatgcggcg tccggagaaa gacccagtat aagtcaagcg
    46681 tttcggaatc aaaatcatct ctgattcgga aattaccact atagggaata tcagctttga
    46741 ttggtttttc caatataatc ggcagaggat aggaatattg aatctcggcg tggtcaggat
    46801 caatcaccgg ccagccgtca atccatttta ctggtgccat aaacgtttct cgaccagtat
    46861 tgaaaaaacc ttcatattcg ggaagataag gtcgacagcc gagaaataca gcccaccaat
    46921 cgccagcggg cgtcatcacc aaatcggcgt gaccggtaca ggtaatcgga tgaggacgat
    46981 tcggatcaag atgacgttga gtcagaatcg gatttttcgc ataaggaaca tacggtccgg
    47041 taacctgttc gctccgaaag actacctcgg aatgttccca agatgtacct ccttcggcac
    47101 aaatcagata atagaaatta tccttgcgaa aaatatgcgg tccctcgatc cagatcggct
    47161 ttttagaaat atcggtacca ccattcacca ggataatctc aggactaatt accttaagtt
    47221 tttcgtagtc aaattgatac atccggatcg tgcgatgccc ttcgtataaa ggtctattgt
    47281 tcggcgcgac gctgttataa acgatatacg ctttgccatc ttgatcgaaa aatagcgagg
    47341 gatcaatgcc gtcaatttgt ggaagccaaa ccggattcga ccacggtccc gccggatttt
    47401 gagcagtaac gacgtaattt ccaccggcac caaccagagt acaggttata taaaataatc
    47461 catcgtgaaa tcgaatcgcc ggagcgaaaa ttccttcgga cacgcccaac ccttccagat
    47521 tcatctgctc cgctcgatcc attacatgac cgatcagttt ccaattgact aagtcgcgac
    47581 tgtgaaaaac cggtattccc gggaaataag caaaggtgga attaaccaga taatagtcat
    47641 ctccaacccg acaaatactc ggatcgggat aaaagccagc cagaattgga ttatagtaga
    47701 acgctttttg atcagctgaa aaagcgccgg cgcttataaa actcgcaact attagcgtca
    47761 ttgtaatcat cacttttttc atttgatacc tacactttta gaattgaaaa cttttattct
    47821 gcaattttaa accagcggct ttccggcggc ccgaggtaac tgggttttac ttcaccagtc
    47881 tcaattacca atttttggag taccacgccg ggatcgaccg cccagaattt taaggtatgt
    47941 ttgccgggcg acttcaacgc gtggacggaa gtcattatcg taatatagtc actgacggaa
    48001 ttttgccagt cacggtgggt tttgccagca tgcatattga tgatttgaac cggctcatca
    48061 tcaaaagaaa tcccatagcg taagcccgga ccattgggaa aattctgggt tggagccaga
    48121 taagccttaa ctttcacttc accagcatta aacaaataaa cttggtactc aagatgtgga
    48181 ctatcgcctt ccggtatcag actggacatc gtcaccggaa atggagtaac tgaggaaagc
    48241 gtacgtccca aatcgggaat aatttgccag taaacaccac gcgtgttgac ggcattggaa
    48301 aaatgctcag cttctattga aacatatctg ttgctctcaa gaaaacggtc ggcaagctcc
    48361 tctacaggaa ctgccggatt aaagataatt attttcacct ccacctggct tttccgagga
    48421 cctttgataa tgagcggaat tgtgctcttc cctttgggcg cttgcttcca atcaatttca
    48481 atccagagcc gttcttcggt agtaatttct ccttttttct ggctaattag aacgcaggga
    48541 ttgccggatt taatcgtgta tttaaaaggc tttttacccc gattaaaaat ttcgatataa
    48601 tgacaggatt gattgtaggg atcgaattcc ggcaaaatcg cctcgctatt cgcaggcggc
    48661 caacattcgg ttgagccttc aatcgcaaca cccatctcgg ctgattccgg tacattgatt
    48721 tcgattactt ctggcattgt gttttcttcg ggttgttgcc aataagtata gcctatatga
    48781 gtctgatcca tcatatgatt ccacttcccg ttagcgatat tatgataacg atcggttatt
    48841 tcggcatcct tcttaaatag atcacgggct ctttgagcta tttcattggt cagggcgcgt
    48901 ttctgctgag cataaagtcg gttacgcgcc acggtcacat aaagctcatt gagattggcg
    48961 caggcttcgg taggatgcaa aactaattga tagaaagcat cgtgaaattc agtcggcaga
    49021 gcttcgtaaa tttcccgtgc tttttttgcc aggtcattat attcagccac aacggtttcg
    49081 gcttcccgat agttaaccaa actataagtt tcaggtgaga gtaattctgg tttgcggcgt
    49141 gcattgaatc gggtgtactg agtcaatata tgagcaattt cctcagcata ttgagcgccg
    49201 aactgctcct ccgcccagag gcgagtatat tcgggcaagc gctccgccgg ccaggcttcc
    49261 ggattccaag caaaatccat aaaaaagtta atcggaaatt ccatcggttt aatgtcacca
    49321 acattgacca cccagattcg gtcaacacca tatcgataag ccaggtgcat ttgttcccaa
    49381 actcgtgaaa tttgaaccgt attcaaccat ttgtaattgc gcggatcgcc gacataatca
    49441 aaatgataat aaattccata tccgccagcg cgtggtttgt cgtctaatcg aggcaaacga
    49501 cggatattgc cccagttatc gtcactccac atgatagtca catcgtcggg aacctgcaaa
    49561 ccagcatcgt aataatcctg cacttcttta taaggaataa aaacctgcgg aatttccgta
    49621 atttctttat ttatatgttt tgtaagaatt tcgcgctggt ctttgaatac ttgttcaagc
    49681 aaggcgattt gttcctgaac atccactccc ccttccatct gggtatcgtg aattccccgt
    49741 aaacctaccg tataaatatt ttcatattgg ccacgggcgg caacggtttc atccaacact
    49801 ttataaatat tttcacgatt aatatcataa cgccagggtc ccatagtttg tg
//
LOCUS       DAOMJU010000004        46690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_601_length_46690_cov_22.609070, whole genome shotgun sequence.
ACCESSION   DAOMJU010000004 DAOMJU010000000
VERSION     DAOMJU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 46690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46690
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_601_length_46690_cov_22.609070"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1309)
                     /locus_tag="PLO58_00725"
     CDS             complement(44..1309)
                     /locus_tag="PLO58_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73343.1"
                     /translation="MEIIERATLQQHITNTPDSYLRPLLAALYLEDQDCQSAIELCRQ
                     DLAIHPFSTIGHYIWALAALAMNDLNQAAIHLRAIIQIDGKFLQAYYKLTELGGDYLT
                     QAELKYCYEKICDLNPYDKKSTDKLSAFPLELQSQKPQSPAKPERTDQVKESSEKAIM
                     AEPIESAPEIDPLTGANRLSQFFESVIKTTVTPNSSEIKPTPVPEEEAHFGPIEKTAI
                     ETEPTVEPEPTISFDEPFPEEPQVKESSGSSSISDLFSRLREKPFEEVQKENWLKEHL
                     SGETPSPAMPTITESDKPAVEPQAVSQPQSETKATDIPAKSKATATPKVAPTKAGKAS
                     QKPKSASNKQRQSPQPKKSAASPGNISFPIPTWTLVDILMKQKLYEQALNILDIIESK
                     SKNEKDLEKVQASRAKIVRLMAEEQSGEA"
     gene            complement(1530..2243)
                     /gene="radC"
                     /locus_tag="PLO58_00730"
     CDS             complement(1530..2243)
                     /gene="radC"
                     /locus_tag="PLO58_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQO73344.1"
                     /translation="MRSKEPAVYKLPITAWPESDRPREKLMKAGPMKLTEAELLAILL
                     RSGAGGNSALDVAREILRKSGGLNALAQMDYHDILNLKIRGIGPTKAVTIAAGLQLAR
                     RLQAVSVSPPEMIIHKSDEVAKIYIPRLQDLKKEIFMVVLLASNNKIIRDAIISEGIL
                     NASVVTPREVFREAILGMAAAIILIHNHPSGNPEPSNEDLQLTRQMKEVGQAMNIPVL
                     DHIIIAGAQYTSFADKELL"
     gene            complement(2248..2694)
                     /gene="dtd"
                     /locus_tag="PLO58_00735"
     CDS             complement(2248..2694)
                     /gene="dtd"
                     /locus_tag="PLO58_00735"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQO73345.1"
                     /translation="MIAVIQRVSQAHVTVENKIVGRIGPGLVILLGVFQNDGDADAAF
                     LADKIAGLRIFGDEQQKMNLSIKDVGGAALVISQFTLCGDWRKGRRPSFISAADPETG
                     RRLYEKFIADLKIAGVPTEQGIFGAMMAVSLVNDGPVTFVLDSKLR"
     gene            complement(2694..6125)
                     /locus_tag="PLO58_00740"
     CDS             complement(2694..6125)
                     /locus_tag="PLO58_00740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQO73346.1"
                     /translation="MTQFVHLHNHSDYSLLDGAQSIESLVNRAVELGMPAVGLTEHGN
                     LFSAIHFLKAANTSGLKPILGCEIYVADNNRFDKKSREQGGSGYHHLLLLVQNRQGYE
                     NLIKLVSLGYLEGYYYRPRVDNELLRKYNAGLIATSACIKGRIPDLAIKGDYEKAKQL
                     ALEYAEIFAGRFYLEVQNHGIDDELRWYEIAKKLSAEIGLPRLLTNDTHYTLPEHWEA
                     HDAHLCIGTGKEMSDPDRMKYEQHEYWVKSAEEMAALYPNDPEIIENTLKIAEQCDFN
                     LEMNQYYLPEFPIAESAKVKTADEYLAQLIEERLPGRYPEITPEIRERVDYELGVIRQ
                     MGFAGYFLIVQDFVHYAKNNNIPVGPGRGSAAGSIVAYILGITNIDPLKYKLIFERFL
                     NPERISMPDIDIDFCDTKRGAVIDYIRKKYGENCVAQIITFGKMKARAVIRDVGRVLS
                     MPLAEVNRIAKLVPEQINITLETAINNTPELQEAVNIDEQHQKLFAISKVLEGLNRHA
                     STHAAGVVIAPGNLTDYVPLYQSSNGEVTTQYDMKCIDQIGLLKVDLLGIRNLSVIDN
                     TLAMLKAKGIELDIDKIPEKDEETLALFGEGRTIGIFQFESSGMREYLKKLKPSGIED
                     LIAMNALYRPGPMEMINDFIDRKQGRKPITYLHPLLEPILVDTYGIIVYQEQVMQIAH
                     QVAGFSLAKADLMRKAMGKKQKKTMESLKKEFVEGAIKNGIKRSVAVQIYELIQRFAE
                     YGFNRSHSAAYAILAYQCGYLKAHYPAEFMAANLSSEINDTDRITILANEARNMGYEI
                     LPPDVNYSEVRFIPEGKAIRYGLHAIKNVGEKAAENIVATRQTEKQFRTLFQMVAALD
                     LKSVNRKVLESLIAAGAMDSLEGTRGQKFAAIDTAIQYATHIQEEKNNNQVNLFGIAN
                     GAPQATLSEPRLPDVAPWEDSEKWNKEKELLGMYLSGHPLLEYSTEIESFSNYDFTEP
                     LSNLDKSNVKLGGLIASIKTMTDRKGRPMAFINLESLNGKVELIAFADIYEKYREHLK
                     INYPIFVSGNVNCRNEDDAKIIVEEISPLRGLLNRKSKKLHVRINVNQFGAEDLENLK
                     ILLAKHTGECQLFLHLYDGNGVNKSIRSGNLRVSADRELLDILKKTFGEDNAWVE"
     gene            complement(6126..6965)
                     /locus_tag="PLO58_00745"
     CDS             complement(6126..6965)
                     /locus_tag="PLO58_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HQO73347.1"
                     /translation="MKKLLAYFKIMRPLNLLQGGIAILVCMALADPKPEWLSVLLALV
                     IVWAYTGAGNILNDYFDVEIDRINRPQRPIPAGLIQPRTALFYAIFLFALGTAAVVPV
                     FNRQLAIIIPFALFFLITYTKFFKPRPLWGNLIVSAILGMTFLFGSSIFGDIRVGIVP
                     CLLAFGFNFVREIVKDMQDVAGDQARGARTFPLVYGLTAARRLVIVATLILMVGALVP
                     FYLKIYGKFYLLILVLTVEIPLIYVIFSISRDASATNCGRIAAILKGDIFFGLLAIFA
                     GKF"
     gene            complement(6962..7843)
                     /gene="prmC"
                     /locus_tag="PLO58_00750"
     CDS             complement(6962..7843)
                     /gene="prmC"
                     /locus_tag="PLO58_00750"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQO73348.1"
                     /translation="MAERERQIWRIVDVLKWSRDYLKGKQIESPQIEAEWILREVLNL
                     NRLEIYLKYERPLSPAELARIRALLLKRASGQPIQYVLGSAEFMGLKLKVTPDVLIPR
                     PETELLVEKTLELCRNRVTARILDIGTGSGCIAIALAHFLPECQVVAIDVSATALQIA
                     RENAAIHNVANRIDFQELDILHSPELTMGQFDIIVSNPPYVSGDYYVNLPKLVKEHEP
                     EIALNPGTDGLLFYRRIRELASKIAIPGGAVVLEIGGDYQATAVAELFSESAFDSVAI
                     VKDYSGQSRVIIAELAK"
     gene            complement(7951..>8565)
                     /locus_tag="PLO58_00755"
     CDS             complement(7951..>8565)
                     /locus_tag="PLO58_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HQO73349.1"
                     /translation="GTQNGLGGFKEVIFLLSGENVYGTMKFESGVHRVQRVPVTETQG
                     RIHTSAATVAVLPEAEEVDIEINASELRIDTYRSSGAGGQHVNKVETAIRITHLPTGL
                     VVTCQDEKSQYKNKEKALKILRSRLLALKEAEKEAEIAAKRRSMVSTGDRSAKIRTYN
                     FPQGRVTDHRINLTLYRLTEVMEGEIDELISALQLAHNTELMKS"
     assembly_gap    8566..8665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8666..9334)
                     /locus_tag="PLO58_00760"
     CDS             complement(<8666..9334)
                     /locus_tag="PLO58_00760"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HQO73350.1"
                     /translation="MAQKILVGGQAVIEGVMMRVPGAYATAVRRPDGEIEIKRENFTS
                     IADRIPFFKKPLLRGIISMFESLKIGLETIQLSANIAMQAENVPKPADKTKDRIATTI
                     SMILAFGLAFLLFGFLPLWLTTQLLNIERKALAFNLVVGAWRILFFLMYLWLISFMKD
                     IQRLFQYHGAEHKVVFTFESGQELTVENAQPFKTYHPRCGTSFIFIVMMVGILLFALI
                     DAVVI"
     gene            complement(9395..9607)
                     /gene="rpmE"
                     /locus_tag="PLO58_00765"
     CDS             complement(9395..9607)
                     /gene="rpmE"
                     /locus_tag="PLO58_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQO73351.1"
                     /translation="MKPNIHPEYKECTVTCACGNTFVTRSTVGDLKVEICSACHPFFT
                     GKQKLIDSAGRIEKFNRKYKRDTQKG"
     gene            complement(9717..11642)
                     /locus_tag="PLO58_00770"
     CDS             complement(9717..11642)
                     /locus_tag="PLO58_00770"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010884405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HQO73352.1"
                     /translation="MYKSQKTSLKSEDQDSKSDANPFPDCLDTADWRKLTALQNQYVE
                     AIVTEYIRLCRPAKVSILSDTPEEIAYVRQLALRTGEEQPLKIAGHTVHFDGYNDQGR
                     DKEHTRILVNDGHLNSERLNTLDRVKGLEEIHQLLDGIMAGKEMLVRFYCLGPLGSIF
                     SIPALQITDSSYVAHSEDLLYRQGYSEFRRLNGSDRFFHFIHSAGELDERGCSKNVDK
                     RRIYIDLESNRVFTINNQYAGNSVGLKKLALRLAINKANHEDWLAEHMFIMGVHPLQK
                     KRTTYFTGAFPSACGKTSTAMLPGQTIVGDDIAYLRVSNDKKCNAVNIESGIFGIIAD
                     VNPIDDALIYRCLTTPRELIFSNVLVKDGKPYWLGMGCETPESGINHSGEWFKGKKDA
                     AGKEIPLAHKNARYTMRLSELENLDPRADDPNGVPISAIIYGGRDSDTNPPILQALDW
                     LHGVMLGATLESETTAATLGQEGQRTFSPMANLDFVVVPLGLYIENHLKFGARLAKAP
                     LVFSTNYFLKDSSGNYLNEKTDKKVWILWAEGRVHNEYDAIATPVGYLPQYNDLQNLF
                     KLALGKDYSRTAYEQQFTLRIDKFIAKLDRVSEFYRNETIPAEFNNRLDQQRADLVKL
                     QEEYQAPEISPFQIVKR"
     gene            complement(11747..12511)
                     /locus_tag="PLO58_00775"
     CDS             complement(11747..12511)
                     /locus_tag="PLO58_00775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HQO73353.1"
                     /translation="MKVLYSKPQVKTIQIDGLGSVTLRRDHRARRLTLKVKPDQQITL
                     TVPYSISEKTALAFLEANREWALKNIQALKEIKPPIFIIEDKQTIQTRTHRLALQPTS
                     GDEIRVGIQPGIINVQYPSNQKITNPRIQEAIKLGLTAAYRIEAKQFLPPRVRDLAQR
                     YGFRYNRLFIKNLKSRWGSCSAANNINLNLHLMRLPDELIDYVILHELTHTQIKNHSP
                     LFWQELERILPDAKVRRQQLKTITVQGFLPSVVIEN"
     gene            complement(12625..13986)
                     /locus_tag="PLO58_00780"
     CDS             complement(12625..13986)
                     /locus_tag="PLO58_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HQO73354.1"
                     /translation="MKQHKFTLSESEMPKAWYNIAADLPYALPPALHPGTQKPITPDD
                     LAPIFPMSLIEQEVSTERFIPIPEPVLEIYQLWRPTPLVRAYRLEKAIGTHSKIYFKN
                     ESVSPPGSHKPNTAVAQAYYNYKEGVKRLTTETGAGQWGSALAFACNYFGLECVVFMV
                     RISYETKPYRKSMIETWGGRIFASPSQETASGRAILANDPNCAGSLGMAISEAVEVAM
                     NDKNTKYSLGSVLNHVMLHQTIIGEETQKQIAQTGDYPDIIIGCVGGGSNFAGFSFPF
                     IRDKIAGKKLRAIAVEPTACPSLTKGLYSYDFGDTAKLTPLLAMYTLGHNFMPSKIHA
                     GGLRYHGASPLVSSMVKEGLIEARSYQQTEVFKAAVTFARSEGIIPAPETAHAIKATI
                     DEAKKADEEGVTKTIMFNFSGHGHFDMSAYDSYFAGRLEDNHFSDASLQKNLAELADL
                     PKI"
     gene            complement(14363..15583)
                     /locus_tag="PLO58_00785"
     CDS             complement(14363..15583)
                     /locus_tag="PLO58_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQO73355.1"
                     /translation="MKINIYRHSGNIKRLLLVLAIVMIFALLNYTQKIVTRLRQDSAN
                     LIRFYADIYAKAAMDMSGDDFSFIFEEIIQKTSTPMILSDAEAMEPTAWRNIGVPDND
                     RSPATLAEVKKIMQEMDRENQPIPLKYEDRVLQYIHFGDTNMIRQLRRLPYIEIAIVG
                     LFILLGYVGFSLIRENEKRSIWVGMAKETAHQLGTPLTALMGWLELLKDEIGDNANIS
                     EIARDIQRLEKVANRFSQIGSRPVLKPTNINLVINDAVSYYRRRLPQLGPGISLEFIP
                     SGDYIVNLNADLFSWALENLIKNAIDAVSQENGKIRVELHLLHGGKKIAVDVIDNGKG
                     ISKSNRKNIFRPGYSTKQRGWGLGLSLAMRIVKEYHRGQLFVLMSKPNEKTVMRIVLK
                     NPQENQKREQESAF"
     gene            complement(15626..18166)
                     /locus_tag="PLO58_00790"
     CDS             complement(15626..18166)
                     /locus_tag="PLO58_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73356.1"
                     /translation="MSKKTKKSTSAKNVPPQNKKPVNWLATINPRKATILAFLIVLLI
                     LIIFYKPYVIDRLEPAGGDRMASIAQSKFINDYREQTGEIPLWNPAIFCGVPIYYALW
                     SNSFHLDYLISKISKVIDWKLSWFILAMVGMLLIFWLLEFPWYYALLGTIAFLFYPHY
                     QALITVGHFAKVRAVCAMPLAVFGFLYLVKKRNFLSFLLFVIFFSLQLRTQHYQIVFY
                     TLLVFMALGIRQIVEWVKTKQAQKIYVSLGLFVAGLVTSVLMSAQPLFVTNEYTPYST
                     RGGQAINLKEDAAQAEVKSSGVTFEYATRWSLNPKELATLIVPRFYGGTSQEPYTGKA
                     YPQLRGQPIPGYWGDMPFTQSCEYMGILIVILALLGLWYYRKDGVVISLFILLVFSLL
                     LGFGSHFPILYKPLFKYLPYFSKFRVPSMILILVDFILVILSVYGLKGLVEKFDLAKY
                     KSALIVSGFFILFGLFFLLAPGVLSYTSAQDAQFMNNPQVVEMLRTARREFLQADTLR
                     MLVFVICFGALVVLYKMKKIRPDFLIGAIIILVAVDMINVSKRFLRDDELVDTKSLER
                     RYFVETQFDKILKQDQEIFRILGLGNHFQSNDLAYRYQIVTGYSPIKPQLIQDIIDNN
                     LLAGETQSSLNWNVINMLNAKYIIAPGMLNEANLTILDVNQQRKEVLYLNEGVLPRAY
                     FVSEVRFLPSEKDVVAFMNTTEFDPAKMALTSVALDTSAGFDTGGSVQVTDYTPNRVV
                     LNVETERPAFLVLADAYYPKGWTARVAGVETPIYQVNHVLRGVPVPAGNYAVEFKFLP
                     RSYQIASQISTISCDIVWLSLLGVLIYQNREKIKNLKKKRPTPAKSNR"
     gene            complement(18180..19685)
                     /locus_tag="PLO58_00795"
     CDS             complement(18180..19685)
                     /locus_tag="PLO58_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQO73357.1"
                     /translation="MLNYVKSALKNSAIYSIGNVSTKLIGIILVPLYTQHLSLKEYGI
                     LSILEVSSQLLVTIFSANLYTAFFRWYWEKEYSDRQKSIFFTTFLSLIFISGLMVAGI
                     YGFSNRLSVLLFDNSDFNYLIRLMSIAAALQILMDLVSALMRVQEKPVLYTSVNVAKL
                     TISLILTVYFISSLHMGIDGIYKAQIISYVVYFILVSRYLIKNIRVHLEIRVLKEMIR
                     FSLPLMISSVSGILLSIADRFCLKFLGELSYVGIYSLGYKIANTVKVFIISSVNLAIS
                     PIMYRVMDDPGNKRLYSKTMTYFAFGTMICVIGISVFGQEIIKFLAQNEAYWNAYKVI
                     PIISFGLFFEMLKDTSLIGLNITKKTPVIARIIITSSIINIALNLLLIPYFSYIGSAL
                     ASLVTQIIYFLLIYHSAQKYYPIPYEMEKIGKIMLCGMVLIVFGLILSPYALWIRLTG
                     KLLLILSFPFILYLWNFYEPIEIQTIKGAWRKWRNPLNWKKNRSKIKLDRK"
     gene            complement(19737..20282)
                     /locus_tag="PLO58_00800"
     CDS             complement(19737..20282)
                     /locus_tag="PLO58_00800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQO73358.1"
                     /translation="MTSFYNEEEIVKLGLKKYGKNVLISRKASFYSPELIQLGSNVRI
                     DDFCILSGNISIGSYVHISAYTALYGKFGICIEDFVTISGRVLVYSQTDDYSGEVMTN
                     PMVNPNLTRVTGGPVVFKKHSIIAAGCIIFPEVTIGEGAAIGAMSLVNKNLEPWSIYV
                     GIPAKYLKPRSKKIIELEHLI"
     gene            complement(20295..21380)
                     /locus_tag="PLO58_00805"
     CDS             complement(20295..21380)
                     /locus_tag="PLO58_00805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQO73359.1"
                     /translation="MTMINVTKTYLPQKQKYLEYINKIFKSGWLTNNGELVQNLEKRL
                     EDYLGVPHVILTANGTLALQIAYHLLELKGEVITTPFTFIATTSSLVWEKLIPIFADI
                     EPDTFNIDPIQIEKNINNNTSAILGVHVFGNVCNVGKIEEIANSYKLKVIYDAAHAFG
                     VKYYNKSISEFGDVSIFSFHATKIFHTIEGGAIVLKDDDMAKKARLLINFGIPGPDEI
                     ATLGINAKMNEFQAAMGLCVLDDIQDIIEKRHVVYNRYLEAFIDNNKLTLQQIAPHST
                     QNYSYFPVLFESEKILLQIKQILNQAEIFPRRYFYPSLDTLHFIHPKQKMPISRNFSS
                     RILCLPLYEDLAEEIQDQIIKIILMYC"
     gene            complement(21377..22468)
                     /locus_tag="PLO58_00810"
     CDS             complement(21377..22468)
                     /locus_tag="PLO58_00810"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: protein motif:HMM:NF019073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0036065 - fucosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HQO73360.1"
                     /translation="MLTVHIFTRESLYSYKFINFLYKHFDISEHFFIYRSPHKEQYEY
                     PPSFQIKNILYIDSKSKYILKLIPLLLRSQIIYIHFLPIGITPIIWLIFPSLIPKTSW
                     ILWGDDLYWVETRKKGFKAAIYESIRRRVIVKLPRIIAFMEGDYRIAKSRYNTRAIYQ
                     YVVYPLPTDFEYLESIRCNDLVNEEITIQVGNSGDPSNDHIGVLEHLKRFSDRHFKIF
                     CPLSYGGTKEYINSVIQYGQDAFGEKFSPLLEVIQPKDYSKLLLKVNVAIFGSSRQQG
                     LGNILSLLFLGKKVYIRKETTPYEYFKKINVVIFPYSDLENVSFQELAYISPLIAENN
                     YKIIKNHFNEENYVRMWKEVLSIDTNKRK"
     gene            complement(22496..23689)
                     /locus_tag="PLO58_00815"
     CDS             complement(22496..23689)
                     /locus_tag="PLO58_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQO73361.1"
                     /translation="MILDKEFPPDERVENEALILLEGGHEVYVLCYGYSRSQTKIENY
                     RGIVIQRIPTSKGLIKRLRVFQHPSLNLYKNFWERKVKKFVVHNHIDVLHIHDLYMVP
                     SGIKARRYIGIQVVVDLHENYPAALKAYTWANTLTGRLIIRPQDWKDIEAVYFQNVDR
                     IIVLSQYYRIQLLRKYTFLKDDDITVYPNVPCVKGLLNYKIRRVLDKEDNFTLLYFGV
                     IARRRGIFTVFEALRILIQQGRQKIKLLLIGPIDTNEKKEFNKYLSDNTLKDQIIHYR
                     WKDISEMPSYITDSDVCLSPILKNDQHESGIANKVFQYMLFARPVIVSDCGPQKDLIE
                     TTGCGLVHKSDNAIDLAMKIQYLQDNPELRKTMGEKGRKAVLEIYNTEIQGKQLIELY
                     NSIDI"
     gene            complement(23698..24474)
                     /locus_tag="PLO58_00820"
     CDS             complement(23698..24474)
                     /locus_tag="PLO58_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73362.1"
                     /translation="MASNPFNIEKKIRQKSRLNEYRELLLFARDEGYILTSLIEWHRN
                     YRQNGQRVLILRHDVDIDPEGAAKMYAIEKSLGARATYYFRWKTVSEALVHKMRRDGF
                     EVSLHYETLATYCKKHHIKDAKKVTDTVMKTCFNLLEEEISRFKKQFGDIQTIASHGD
                     KRNRILGIPNHAILKYGNRADLDIQFEAYDPEITMLFDAYISDSSITNNFNWKYGLTP
                     QEAIRSHLTTICLLTHPEHWNFNFKKNMKTIITDICERLS"
     gene            complement(24574..>25380)
                     /locus_tag="PLO58_00825"
     CDS             complement(24574..>25380)
                     /locus_tag="PLO58_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HQO73363.1"
                     /translation="LLQNRPGFCQKTLGNSISSLLFSLQNSTKILKWDEYRRGLQMLF
                     SAGDLERCYLILRNCWDYDKRFRQEIYQPSFRQMPLTPTPAYFDPIFEACQDLPPLDA
                     IAYVEFHSKMLNDYILTEDRMSMAHSLELRVPFLDKDLVEFAFTIPAGLKMKNGQTKY
                     LFRKAMAPYLPPEILQKKKRGFAVNPYDQFNKDLKKVAEKVLTREFIQHQGIFNYEYL
                     QRILNYPPDPRLRWHYNFLWIVLGLAIWERMFVETENYRKHIVAPKEYYQ"
     assembly_gap    25381..25480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25481..26217)
                     /gene="asnB"
                     /locus_tag="PLO58_00830"
     CDS             complement(<25481..26217)
                     /gene="asnB"
                     /locus_tag="PLO58_00830"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQO73364.1"
                     /translation="MTDLLKHRGPEKAGYFHDGNVSLGHRRLAIIDLETGDQPIYNED
                     RTVVVIMNGEIYNFPEFKADLIQRGHHFYTNSDTEILAHSYEEYGLEFLARLNGIFAF
                     ALYDIKDERLILARDHFGVKPLHYWFQNEVLLFSSEYKAILVHPAVRRQVNYSALHCQ
                     VNLRYNQSDETLIKGIYRLPPASYLIYEKGQISIKKYYQLPVNLNFKMTEAEAIDGIR
                     FHLQQAVRRQLISDVPLGVYLSGGMDSS"
     gene            complement(26371..27465)
                     /locus_tag="PLO58_00835"
     CDS             complement(26371..27465)
                     /locus_tag="PLO58_00835"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQO73365.1"
                     /translation="MIPFMDLETQYLNIREEIDSAIFNVIHSYKFINGPEVATFEKNF
                     ATAQNLKYMLGLASGTAALHLAYEILGITSGDEVIVPAMTFIATAETVRQVGAKPVFA
                     DIDPVSFNIDCNGLEKLITPKTKAIVVVHLHGNPCEMDRIVSFAKKYNLKIIEDCAQA
                     HLAEFQGQKVGTFGDVAAFSFYPGKNLGAYGDAGALGTNDETLYEKAKLLANHGRKEK
                     YVHQTEGYNYRLDTLQAAILDVKLRHLPEWTEQRINIANTYHSLLKNLPITLPEMNPA
                     KKHVYHIFAIATDRRQRIIEALKANQIAFGIHYPLPLHLQPAYSYLHYKPGDLPNSEL
                     LSTQFLSLPMYAELSRDNIEKICQVIASVY"
     gene            complement(27481..27945)
                     /locus_tag="PLO58_00840"
     CDS             complement(27481..27945)
                     /locus_tag="PLO58_00840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQO73366.1"
                     /translation="MAEPIIKKIGIRDIKCGTNVVIIEPCNLYECEIGDNTFIGPFVE
                     IQKGVTIGRRCKIQSHSFICELVTIGDDCFISHGAKFVNDTFKSGKPAGGNRELWKPT
                     IIGNHVAIGTNATILPVKITDRVVIGAGSVVTKDILHSGIYAGNPARKIRDL"
     gene            complement(27948..28958)
                     /locus_tag="PLO58_00845"
     CDS             complement(27948..28958)
                     /locus_tag="PLO58_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQO73367.1"
                     /translation="MKTIKFALIGCGRIAQRHAEQIHRLGELSAVCDIKESAALALSE
                     KYNCRPYFNIDTLLSNEPDVDVVSVCTPNYLHAEHSIKSLKAGKHVLCEKPMALSVRD
                     CENMIYTAEQTNRRLFIVKQNRYNPPVVELKKVIEENRLGKIFYVQLNCFWNRNEDYY
                     LQSDWKGRLDKDGGTLYTQFSHFIDLLVWLFGDFKNVKVLLQNYLHPNIEFEDTGAVL
                     IEFFNGAIGTINYTVNSYQKNMEGSITVFGSKGTVKIGGQYLNALEYQNIEDYSFQNV
                     TPVNKANDYGFYQGSMSNHDKVYENVVNVLNGKDTIATNAIEGLKVVRIIEDIYSQSK
                     LK"
     assembly_gap    28975..29074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29228..30526)
                     /locus_tag="PLO58_00850"
     CDS             complement(29228..30526)
                     /locus_tag="PLO58_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQO73368.1"
                     /translation="MKKVLFISYYWPPAGGATTTRILKFYKYLPEFGWEPIVLTVENG
                     DFPFIDPQTLKEVRPDTKIYRAKNISLHRIFSIVAKNSKEMFVPFAFTEKNNKGLKGK
                     LSRWVKYNAIPDTRFLWKRFAIKKGLQIIRENDIKLIFSSSPPQTNHCIAVELSRKTG
                     IPCVGDMRDPWTDVYWLQTNSQRWKWIHNLDQKIEKRTLNSMAAITTIGPSLVNLFQK
                     KVNKPVYLIYNGFEPLDVDFVPRKNAKWTITYAGSISIEQDIQAFCKAIEVLVQNENI
                     RENITIQFIGNFPEFVKQKLRQYSVFEMITFLPYMPADEVKRYLVSSDVLLLFIHVTP
                     EGGVINYKMYDYLATYKPILAYGPTQGDAAKILHRANAGRMFEYDNVADSANYLLSLY
                     EKWEKGEPAVEIDKEYVMTFTRRNLTRQLAEIFDKYYRKS"
     gene            complement(30526..31290)
                     /locus_tag="PLO58_00855"
     CDS             complement(30526..31290)
                     /locus_tag="PLO58_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQO73369.1"
                     /translation="MPKIEQQPTKKDVEEFWNRNVCQTEFLKTGERGTEEFYQEAERV
                     RYKYHFYLPELFDWIAKEKPNGSLLEVGCSMGTDLLQLARRGMRVTGIDLTEEGINLA
                     RKRFELYQMPAELKVDDAENLSFPDNTFDVVYSFGVLHHTPDTQKAIDEVWRVLAPGG
                     LAVIMLYNRKSLNYYIHRLINQPFDGNRKDRCPIERTYTKAQIFAMFKNFKVVKIETE
                     YLLTTGYGIVWDLIPKAVHRALGHLWGWHLVIKAYK"
     gene            complement(31262..32494)
                     /locus_tag="PLO58_00860"
     CDS             complement(31262..32494)
                     /locus_tag="PLO58_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73370.1"
                     /translation="METNNKSLEQVIKTVNDSLDAVLSYSQRNDYAGFNKYDALSSPI
                     LLPLSFGNKNLRLFYSQAIMRSPLNLRPLFAIPRTRNPKGIALFAMTYLNLYLARKSE
                     KFLQKAEELLDWLVQNQSTGFPGNSWGYQYPWQDVGFFAPPNLPNRVVSYFVGTAFLN
                     AYEITGKNLYLNYAIGVKDFLIQAPKVLYEDDKMKCLSYVPDESINWVVMDVSALCGA
                     FCARLAHFTNDKSLIEEARKLISYVVDKQTDYGAWFYTHPPTANRLKMHDNYHTGYIL
                     DAILDYIDFSGDDSFLGNYHRGLQYYLENLFLADGTPKWMNNKIYPIDVHGSAQGIIT
                     FLKAGRFNRQNEEFAYRIAEWAINNMQNKQEGFFYYQKGRFFKKPFTIMHWSNGWMAI
                     AMSEIIRKYYAENRATTN"
     gene            complement(32564..33346)
                     /locus_tag="PLO58_00865"
     CDS             complement(32564..33346)
                     /locus_tag="PLO58_00865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQO73371.1"
                     /translation="MTNTPPRNLNFHKEQYWTAANFLEKYISISPVSGLRILEVGSAE
                     GGGLNYFATKNNQCYGLEYSRGRLEIARELNKSSKIIFIHGDITEASSYENQIQDKMD
                     LIICGDVIEHIANGKKAAALQNMRDLLQKGGRIYISFPPKYSPFAGHQQGLPGWLHRI
                     PYLFLLPDKFYSAILKSQGIRADIISNMLATKHSRLSIKQFEKMVYENGLRILKKDFF
                     LIRPRFKYNYGIPYIGHRCDLPIVREIFNLGAVYLLQPNAKN"
     gene            complement(33343..34377)
                     /locus_tag="PLO58_00870"
     CDS             complement(33343..34377)
                     /locus_tag="PLO58_00870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO73372.1"
                     /translation="MPKYRFSVIIPSYNRADEVIDLLKSLQAQTCEHSRFEVLVIDDG
                     STDETEQKVNELASQVDFRLRFFRRNHEGPGPARNFGMSQAEGEYFLFIDSDCQADAN
                     WLKEIDEAITKEAPDAFGGPDSVLDSFTPLQKAIDYSMTSFLTTGGMRGHSQKRLAKF
                     YPRSFNMGIRREIYEKLGGMGNLRHGQDIEFSRRIVASGAKVIYIPNAIVYHKRRTSL
                     RKFFRQMFNWGVARINLSYLDAGNLEPLHFIPAIAAVGYTLLTIFTLLTGLAWKIWVA
                     ITVLALLICGYIFIDSSIKYKSVKVGWLAPIFAVCQIFAYGYGFTYNYVRRKIFRRGE
                     VTGFVRRYYQ"
     gene            complement(34377..35303)
                     /locus_tag="PLO58_00875"
     CDS             complement(34377..35303)
                     /locus_tag="PLO58_00875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQO73373.1"
                     /translation="MNSENEKKLVTVVIPVFNEVESLRELNEKIQAAFRSTNYNLEII
                     IVDDGSRDGSAQVAREIASTFPNVTVTGFQRNRGKAAALNEGFRLAKGDFVATIDADL
                     QDDPEAIPAMLDYLQESGYDLVSGWKQKRQDKFIKKTTSKIFNSVTRLLTGVKLHDIN
                     NGLKVYRAEVVKSIYLYGELHRFIPVLAKFEGFTSGEYKVKHFPRKYGKTKYGVSRFY
                     KGFLDLLTVIFITKFMRRPMHFFGLWGIVIILIGLLSELWVLILKFGFQEPFKDHVAL
                     LIFGVLAIVFGMQFFSLGLISEMITFQNRKEK"
     gene            complement(35329..36057)
                     /locus_tag="PLO58_00880"
     CDS             complement(35329..36057)
                     /locus_tag="PLO58_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73374.1"
                     /translation="MKTLSVNCNRKFTGLLISLILSFSGWNCSRAPLKHEKVDEHWAP
                     ALRERSRQLKQLANLSGSGQASFSGTAGSGIIYFDFEYRTADTLNLWIKDPIGRKLAN
                     LELGGDTYHLHLIRDGRYFSGTQLETDQLPISCGPITPALIRRILLAVPFESTTNETA
                     SQFNEASDETPVLYYKFKPGQGEPAELNWLYKNKQIIIVYKEYADVARLKIPAQIVIK
                     NKAEEIAIEIHFSHFNAEMLKFRT"
     gene            complement(36026..37543)
                     /locus_tag="PLO58_00885"
     CDS             complement(36026..37543)
                     /locus_tag="PLO58_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73375.1"
                     /translation="MKKLYPSRATTILRKIVGLLVLAALVSCAATKQTSKSAEKPAES
                     SQNTQYNSQALTHFMNGENYALQGDYAMAVLEYQDALLYETKSAAIYSSLGKAYINLG
                     KLERGLAALEKACQLDPDDAETREILAQIYFLTGRFQQAEKQYQALMTLRPNEPEIMY
                     QLAAIYLKNRKIDQALQLYDEIVKVDSTQYQALQKAAEIAILNRDLNKATAYYDQLVK
                     LFPDSLLYLKTRADLAALGNDFETAIDIYKKLIILNPGDIELRERLGEILCWSDHFEE
                     AQTILEAIIDANPERKASYANLALLYVRNENYQQALDILDSARIRFPNDPSFLLAQGS
                     IYNTLRDYQSAEKVLLKTLQIEPNNVQAQHLLAAIWDMLKKYSQCDSLYEVIIRHNPD
                     DVIALNNYAYSLAVRGERLDEALKMVDLALAKEPNNASYLDTKGWILYRQKKYKEAIK
                     YIQKSLEIEQDNAEVLEHLGDVYQALRQPKKARIYYQRAAEIDKNNENLKRKLQP"
     gene            complement(37612..37815)
                     /locus_tag="PLO58_00890"
     CDS             complement(37612..37815)
                     /locus_tag="PLO58_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73376.1"
                     /translation="MSKGVKGFADKVAKSSKDNKTYCPVCGESVEYVKHIETTSDPSN
                     KRSMRFKEQIVGVCKCNRDQIYQ"
     gene            complement(37828..>39406)
                     /locus_tag="PLO58_00895"
     CDS             complement(37828..>39406)
                     /locus_tag="PLO58_00895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HQO73377.1"
                     /translation="SRCDLAEKTLDSRRSSISRDDEFAGELSLKLKIDPDQNYNEIIA
                     QILKIAQSSLMARSAFLYLFNADEHTYILQAYQSQEGIEFRPKFKAEGTIFRELHLTL
                     KPQIYSADGLKEGSLVYYTVPPKVGTIMVVPIMFNQEVFVGVLGLDSIDRNAWGHEDV
                     DLVKLFAELFSSSIWQIDAIDRLNTQIAFFKALCRLNTELPIGIESLELYKEASQVLH
                     KFFNFDKLTFATLPEEESPELYIEYVEGYDTDYSIGHRISWKGGLWEKIIKNGEILLL
                     NDYEQEKLSFRFQPGDLGILPFRSCLGVPLEVGRKRLGGILMESYQPNNYLRDESDTL
                     VLFAKNLSEIINRMNTYQTVKDLAMIDGLTGLYNRRAFEERLQIEIERSRRYENSLTL
                     MILDLDKFKRINDTYGHIFGDMVLKKTASIIRGSVRTVDTVARYGGEEFAVILINADK
                     NNSYKSAERIRSNIQSFVFEKDGIREHITISIGMAEYPMDGTDVPTMISHADLAMYQS
                     KRLGGNKVTLFKKEYAT"
     assembly_gap    39407..39506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<39507..39952)
                     /locus_tag="PLO58_00900"
     CDS             complement(<39507..39952)
                     /locus_tag="PLO58_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73378.1"
                     /translation="MLSLPKISVSDDFKLNLRNRILSDRSAATQPNSHKGFNLSRIPS
                     FAYGFAVAVVAVVVGFFILQSQSQENSPDNMPPIVRQKMTQSPAQQPQDVTPNNPNQS
                     GLQSPQYTSKQNHPIPSDTATSNNSSSQPAEPTFEYEPNYEDQIKTV"
     gene            complement(40142..40723)
                     /locus_tag="PLO58_00905"
     CDS             complement(40142..40723)
                     /locus_tag="PLO58_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQO73379.1"
                     /translation="MNPEFQLTDEELISRFQAGDEGAFEEIVNRYADRLLNFAYRFVL
                     DREEAEDIVQDTFLKVYQNRHAYREIAKFSTWIYTITGNLAKTILRKRKNRKMFPFSR
                     TASEDKEREIEYPSTERPPDTKLAGEIEEQIIQRAIIKLPEHFRTVIILRDIQDLSYE
                     EISNIIDAPLGTVKSRINRARLRLQEELEHLRR"
     gene            complement(40737..41708)
                     /gene="holA"
                     /locus_tag="PLO58_00910"
     CDS             complement(40737..41708)
                     /gene="holA"
                     /locus_tag="PLO58_00910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQO73380.1"
                     /translation="MSKSDFLSELARIEQGHLDAVYLLRGGDLYLEDEAINTICQAFG
                     KQESERPEKLIYYGGVDSDLEFIDNLFSMGFFARRRIIIYKNIGNLEPNLRARLFNFI
                     ENPGQDTVLIMTADGESHSSLIEQLRKESRKVKTISTWTPKPSRFDEFIRRHLQKSQY
                     SIRPEALKLLIELTDDALSHTIGELEKLLIYIGERKMIEEDDVRLIVGGDKNYDMINF
                     LEAVAHRDITKAIKIGLALIQADASIPYFTTQLYDLFSGIWDYEGHGQKEKFWKKSNC
                     YELGIKNYKQADFGAIFTCIRNVDLQSKSLNLNKEDLLIQLLYEIIS"
     gene            complement(41747..42556)
                     /gene="uppP"
                     /locus_tag="PLO58_00915"
     CDS             complement(41747..42556)
                     /gene="uppP"
                     /locus_tag="PLO58_00915"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HQO73381.1"
                     /translation="MNYFESFILGIVQGLTEFLPISSSGHLVIVQHLFGLATDNIAFE
                     VMVHAGTLLSVLAVYYNDVWRMIVSFCRGIFRLRKAQPFQNDSYFRLSIFVIVATIPA
                     VIFGLTLDDFFEQIFHSVFLVGITLTITGLILLSTRWVKTEGRRLTFGKSLLIGCAQA
                     VAILPGISRSGSTISGGLWCGLDRMEATRFSFLLSVPAILGALVLHLKDLIGMALNPN
                     MIGILILGFLTSFIVGYIAIRFLLRVVQSDKFSWFGIYCIIVGLTVVFFLE"
     gene            complement(42988..44445)
                     /locus_tag="PLO58_00920"
     CDS             complement(42988..44445)
                     /locus_tag="PLO58_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HQO73382.1"
                     /translation="MKEAIEGLKPASLWRYFSEMSQIPRESKHEEAVIAWLESVAKKL
                     NLPYRKDDVGNIVISKPASPGYENRTPVVIQGHVDMVCEKNSGTVHDFRKDPIELVRD
                     GEYIRANGTTLGADNGIGVAAALAILEDKTLAHPPLEALFTIDEETGLTGANSLKPGF
                     IKGKILLNCDSEEDGALYVGCAGGKQTDLFFDINWEKAPQQMANVALKISGLKGGHSG
                     LDIDQGRANAIKQLNRIVWKAAEKYDFRLYYLEGGSKHNAIPREAVYKLLIKPADLAN
                     FETEIAKFEKILNNEYKDVERGIKLTVENLNEYPKQVFTKEFQARVLNCLYAIPHGVK
                     RMSPTIKGLVQTSNNAAIVSTSDKQIRVLTSQRSSVASEIEGLADKVKACGLLANAEV
                     VQSGGYPSWTPNMDSKILTLMKKVYADLFGVEPQVKAIHAGLECGIIGATSPGMDMIS
                     FGPTIEGAHSPDERVEIKTVEKFWRLLVETLKRIE"
     gene            complement(44496..45698)
                     /gene="rocD"
                     /locus_tag="PLO58_00925"
     CDS             complement(44496..45698)
                     /gene="rocD"
                     /locus_tag="PLO58_00925"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HQO73383.1"
                     /translation="MLRSQDYIKIEDQYGAHNYQPLDVVLTRGQGIWLEDVEGKKYMD
                     FLAAYSAVNQGHCHPRLVKVMQEQCARLTLTSRAFRNDQWPLLAKELCELTGYEMVLP
                     MNSGAEAVETAIKAARKWGYLYKGVARDKAEIIVCTGNFHGRTTTIISFSTDEQYKDG
                     FGPLTPGFVAIEYGSIEELKRAINPNTVAFLVEPIQGESGVIVPPEGWLKASSEICRE
                     NKVLLIADEIQSGLGRTGKLFACEHENVRPDVVIIGKALSGGMYPVSAVVASQEILGV
                     FKPGDHGSTYGGNPLGSAIAREALKIIIDEKLPQKSAESGAYLMQKLNEIESPYIEQI
                     RGKGLWVGIVLKPSAGGARHFCVALQERGILAKETHENVIRLAPPLNITRSELDQAIA
                     ILQEVLTQ"
     gene            complement(45868..46533)
                     /locus_tag="PLO58_00930"
     CDS             complement(45868..46533)
                     /locus_tag="PLO58_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HQO73384.1"
                     /translation="MKRHFYWLKLIICEALMFNLNNLAAESGEIRQLPKPDAVGRVPI
                     ETTLSQRRSVRTFQNEPLSEKEIAQLLYAAQGISDSSQGLRTAPSAGATYPLEIYLVT
                     AEGIFHYLPERHAIVLKKAGDCRDHLAEASLGQNSVRTAPAIIVFSAVYERTMRRYHK
                     RGERYVHIEVGHAAQNVHLQAVALGLASVPVGAFNDEAVARVIGVERDERVLYLVPVG
                     RKR"
     gene            complement(46546..>46690)
                     /locus_tag="PLO58_00935"
     CDS             complement(46546..>46690)
                     /locus_tag="PLO58_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HQO73385.1"
                     /translation="RKGVCCDCVRYHLRSRQLPACFFPDDVERTYDRSFEKFTELVQR
                     GKV"
BASE COUNT        13210 a        10139 c         9285 g        13656 t
ORIGIN      
        1 gtttaagtgc tcctaagaaa ttaaaatatt tcctctgctg aaatcaagct tcgccggatt
       61 gttcctcagc cattaatcgc acgattttcg ctcggcttgc ttggactttt tccaggtctt
      121 tttcattttt agacttggat tcgattatat cgagaatgtt aagtgcctgt tcatagagtt
      181 tttgtttcat tagaatatca accaaggtcc aggtgggaat tggaaatgaa atattaccag
      241 gcgaggcggc gctttttttc ggttgaggcg actgacgttg cttattagaa gctgatttcg
      301 gtttttggct ggctttgcct gctttggtgg gcgcaacttt gggggttgca gtcgccttac
      361 ttttagccgg aatgtccgtc gcttttgttt cgctctgagg ctgagagacg gcttgaggtt
      421 cgacagccgg tttgtcagat tcagttatag tcggcattgc aggcgacggc gtttcaccag
      481 acaaatgttc cttcagccaa ttttcttttt gtacttcctc gaatggcttt tcgcgcagac
      541 ggctgaataa gtcgctaatc gaggaagagc cggatgattc tttaacctga ggttcttccg
      601 ggaagggttc atcgaagctg atggttggtt ccggttccac tgtcggctcg gtttctatcg
      661 ccgttttttc gattggcccg aaatgggctt cttcttccgg gacgggtgtt ggtttaattt
      721 cagaagaatt tggtgttacg gtagttttta ttactgactc gaaaaattga gaaagacggt
      781 tagcgccggt gagcggatca atttccgggg ctgattcgat tggctcagcc attatcgcct
      841 tttcgctgga ttctttgacc tgatcagttc gctccggttt ggctggcgac tgcggtttct
      901 gagattgtag ttcgagagga aaggcagata gtttatcagt acttttttta tcatagggat
      961 tcaagtcgca aattttttca taacaatatt ttaattccgc ttgagtaaga taatctcccc
     1021 ccagttcggt caatttgtag taggcttgta aaaatttccc atcaatctga ataattgcac
     1081 gaagatgaat agcggcttgg ttcagatcat tcattgctaa agccgccagc gcccaaatgt
     1141 agtgtccaat cgtggagaac ggatgaatgg caaggtcttg gcggcagagt tcgatcgccg
     1201 attggcagtc ctgatcttcc agataaagcg ccgccaagag gggacgcaaa taactgtccg
     1261 gcgtattggt aatatgttgt tgcagagtcg ctcgttcgat aatttccatc acaaacctct
     1321 tttgattctc actctcttcc ttgtttttta attacttccg tctatggtga ggaaggagaa
     1381 tctgtaatta ttaaaaagtt gccaagttgc tgtaagcctg tgagtaggta ttataccaga
     1441 ttgaaccttt cccgtaattt ggaattgagt attattagtg ttcatttgta atagcttttt
     1501 cctgcttcaa aattaatata aacattttat cagagcaact ctttatccgc aaaactggtg
     1561 tattgagcgc cagcgatgat aatgtgatcg agaaccggaa tattcatcgc ttgaccaact
     1621 tccttcatct ggcgagtcaa ttggaggtct tcattgctcg gctccggatt cccgctggga
     1681 tgattatgaa ttaaaatgat ggctgccgcc atccccagaa tggcttcgcg gaaaacctca
     1741 cgcggagtaa cgactgacgc atttaaaatt ccctcagaga tgattgcatc acggataatc
     1801 ttgttgttac tagctaacaa gacaaccata aaaatctctt ttttcaaatc ctgcagacgg
     1861 ggaatataaa tttttgccac ctcatcgctt ttatggataa tcatttctgg cggagatacg
     1921 ctgacagcct gcagacggcg tgctaattgc aagcctgccg caatggtgac ggctttagtt
     1981 gggccgatac ctctaatttt cagattcaga atatcgtgat aatccatttg agccagagca
     2041 tttagtccac cgcttttacg cagaatctca cgagcgacat ccagagccga attgcctccg
     2101 gctccactac gtaataaaat agccagcagt tcggcttccg tcaatttcat aggtccggct
     2161 ttcatcagct tttcacgcgg gcggtcgctt tccggccagg ccgttattgg gagtttatac
     2221 acggcaggtt ccttagaacg cataatttca ccgtaattta ctatcaagta cgaaagttac
     2281 cggaccgtca ttgactaaac taaccgccat cattgctccg aagataccct gttcggtcgg
     2341 aaccccggca atttttaaat cagcaataaa tttctcgtag agccgccgtc cggtttctgg
     2401 atcagcggct gagataaagc ttggccggcg gcctttgcgc caatcgccgc ataaggtaaa
     2461 ttgcgaaata accaatgccg cgccaccaac atccttgatt gagagattca ttttttgctg
     2521 ttcatcccca aaaatccgca aaccagcaat tttatcagct agaaaagcgg catctgcatc
     2581 accatcattc tgaaaaacac ccagtagaat cactaatccg ggcccaatcc ggccgacaat
     2641 tttgttttcg acggtcacgt gggcttggct gactctttga ataactgcga tcattattcc
     2701 acccaggcat tatcttcgcc aaaagttttc tttagaatat cgagcagttc gcgatcggcg
     2761 ctaactctca gatttcccga acggattgat ttgttgacgc cattaccgtc atagagatgc
     2821 aggaacagct gacactcgcc ggtatgtttc gcaagtaata ttttgagatt ttccagatct
     2881 tcagcaccaa attgattgac attgatgcgc acatgaagtt ttttggattt gcggttaagc
     2941 aaaccgcgca aggggctgat ttcctcgacg ataatcttgg cgtcgtcttc attccggcaa
     3001 ttgacatttc cgctgacaaa gatgggataa ttgattttta aatgttcacg atacttttca
     3061 taaatatcgg caaaggcgat taattcgacc ttgccgttca ggctttcaag attgataaat
     3121 gccatcgggc gacctttccg atcggtcata gttttgatgc tggcaatcag tccgcctaat
     3181 ttgacattgg acttatccag attggatagc ggctcggtga aatcgtaatt ggaaaaggat
     3241 tctatttccg tcgaatactc caatagcgga tgcccactta aatacatacc gagcagttct
     3301 ttttccttat tccacttttc ggaatcctcc cagggagcta catccggtaa acgcggttca
     3361 ctcaaagtag cctgcggcgc accgttagcg attccaaaaa gattcacctg attattgttt
     3421 ttttcttctt gaatatgcgt agcgtattga atagcagtgt caatggcggc aaatttctga
     3481 ccgcgagtgc cttccagcga gtccattgca ccagccgcaa ttaagctttc gagaacttta
     3541 cgattgacac ttttcaaatc cagagccgct accatttgaa aaagagtacg aaattgcttt
     3601 tcggtctggc gggtggcgac gatattttca gcggctttct cgccgacgtt cttgatagca
     3661 tgcaaaccat aacggattgc tttcccttcc ggaatgaagc gaacttcgct ataattcaca
     3721 tcgggcggta agatttcgta acccatattg cgggcttcat tggcaagaat cgtgattcgg
     3781 tcggtgtcat tgatttcact cgagaggtta gccgccataa attcagccgg atagtgagct
     3841 ttcaaatagc cgcactgata ggcgagaatg gcataagcgg cagaatgact gcgattgaat
     3901 ccatactcgg cgaaacgctg aattaattca taaatctgga cggcaaccga acgcttgatg
     3961 ccgtttttga tcgcaccttc aacgaattcc tttttaaggg attccatagt ttttttctgc
     4021 ttcttgccca tagctttgcg catcaaatcg gctttagcca gcgagaagcc agccacctga
     4081 tgggcaattt gcataacttg ctcttgataa acgataatgc cataggtatc cacgaggatc
     4141 ggttccagca agggatgcaa ataagtaatt ggtttgcgac cttgcttgcg atcaataaag
     4201 tcattgatca tttccatagg tcccggtcgg tagagcgcat tcattgcgat caagtcttcg
     4261 atgccggacg gtttcagctt cttcaggtac tcccgcattc cggaagactc aaactgaaaa
     4321 atgccaatcg tgcggccttc gccgaaaaga gcaagggttt cttcatcctt ttccggaatc
     4381 ttatctatat cgagttcaat gcccttagct tttaacatcg cgagtgtatt gtcaataacg
     4441 ctgaggttgc ggattcccag caaatcaacc ttgagcaagc cgatctgatc aatacatttc
     4501 atatcatatt gggtggtgac ttcgccattg cttgactggt aaagtggaac gtaatccgtc
     4561 agattgcccg gggcaattac gactccggcg gcatgggtgg aagcgtgacg gttcaagcct
     4621 tccaaaacct tggaaattgc aaacaatttc tgatgctgtt catcaatatt gacggcttcc
     4681 tgcagttccg gagtattgtt aatggcggtt tcgagagtga tattaatttg ttctgggacc
     4741 agtttagcga ttcggttaac ttccgccagc ggcatactga gaacccgtcc gacatcgcga
     4801 atcaccgctc ttgctttcat tttcccgaaa gtaatgatct gagccacaca gttctcgcca
     4861 tatttcttgc gaatataatc aataacggcg ccgcgtttgg tatcgcaaaa atcaatgtca
     4921 atatccggca tagagatacg ctcaggattt aggaaacgct cgaatataag tttatatttt
     4981 aggggatcaa tattagtaat gcctaaaata tatgccacga tactgccagc ggctgaacca
     5041 cgccccggtc cgaccgggat gttattattt ttagcataat gcacaaaatc ttggacaatc
     5101 agaaaataac cagcgaaacc catttggcgg attactccca actcataatc cactctctcc
     5161 cgaatttccg gcgtaatttc cggatagcga cctggaagtc gttcctcgat cagttgagcc
     5221 agatattcat cggcagtttt taccttagcg gattccgcga taggaaattc cggcagataa
     5281 tattggttca tctccagatt aaaatcgcac tgttcggcaa ttttcagcgt attttcgatt
     5341 atctcaggat cgttgggata aagagccgcc atctcttcag ccgatttaac ccaatactca
     5401 tgttgttcgt atttcatccg gtcaggatcg ctcatttctt tgccggtgcc aatgcaaaga
     5461 tgggcatcgt gagcttccca atgttccggt aaagtataat gagtatcgtt ggttagcagg
     5521 cgcggcagac cgatttcagc ggacaatttt ttagcaattt cgtaccaacg cagttcatca
     5581 tcaatgccat ggttttgcac ttccagatag aagcgaccag cgaaaatttc agcgtattcc
     5641 agcgccaact gcttggcttt ttcataatcg cctttgatgg ctaaatccgg aatgcgccct
     5701 ttgatacagg cgctggtagc aatcagtccg gcattatact ttcgcagaag ttcattgtcc
     5761 actcttggcc gataataata tccctccagg tagccaagac tgactaactt aatcaggttc
     5821 tcatatccct ggcgattctg aactaatagt agtagatgat gatagccgct accaccttgt
     5881 tcgcgcgatt ttttatcaaa acggttatta tccgccacat aaatctcaca tccgaggatc
     5941 ggcttcaagc cacttgtatt ggcggcttta aggaaatgaa tcgcgctgaa gaggttgccg
     6001 tgttcggtca agccaacggc tggcataccc agctcgactg cccgatttac cagagattcg
     6061 atagattgag ctccgtccaa tagactataa tccgaatgat tgtggagatg aacgaattgt
     6121 gtcatttaaa atttccctgc aaaaatagcc agcaggccaa aaaatatatc acctttcaaa
     6181 atcgccgcta tacggccgca attggtcgcg gaagcatcgc gactaattga aaatataaca
     6241 taaatcaacg ggatttcaac cgtcaaaact agaatcagca aataaaactt tccgtatatt
     6301 ttcagataaa aaggaactaa cgcgccaacc ataagaatca gcgtagccac aattaccaga
     6361 cgacgggcgg cggttaatcc gtaaaccaga ggaaaagtcc gcgccccccg agcttgatca
     6421 ccagcaacgt cctgcatatc cttaacaatt tcacgcacaa aattaaaacc aaaagccagc
     6481 aaacaaggaa caatccctac ccgaatatca ccaaaaatgg atgaaccgaa taaaaacgtc
     6541 attccgagaa tcgccgatac aatcagattc ccccataacg gacgcggttt gaaaaattta
     6601 gtgtaggtta ttagaaaaaa taaggcgaaa gggatgatga ttgctaactg gcgattgaaa
     6661 accggaacta cggcggccgt tcccaaggcg aataaaaaga tagcataaaa taatgcggtg
     6721 cgtggctgaa ttaagccagc cggaatcggt cgctgggggc ggttgatgcg atcaatttcg
     6781 acgtcaaaat agtcgttcag gatgttgccg gctccggtat aagcccagac aatgaccaga
     6841 gccagcaaga cactcagcca ttccggtttt ggatcagcca atgccataca gaccaaaatg
     6901 gcaattcctc cctgtagtaa attcaaagga cgcataattt taaaataggc aagcagtttt
     6961 ttcatttcgc cagctccgct atgattaccc gactttgccc gctataatct ttaacgattg
     7021 cgaccgaatc aaaagcactc tcggaaaaaa gttcagccac agccgtcgcc tgataatctc
     7081 cgccgatctc cagcacgact gccccaccgg gaatcgcaat tttagacgct aattccctga
     7141 tgcgccggta aaaaagcaag ccgtctgttc cgggattcag ggcaatttcc ggctcgtgct
     7201 ccttgactaa tttcggcaga tttacatagt aatccccgga gacataaggc ggattagaaa
     7261 caataatatc gaattgtccc atcgttaact caggtgagtg aagaatatcg agttcttgga
     7321 aatcaatgcg attagccaca ttatggatgg cggcattttc gcgggcgatt tgcaacgccg
     7381 tcgcacttac atcaatagct acaacctgac attccggcaa aaaatgggct aaggcaatcg
     7441 caatgcaccc cgatccggta ccaatatcga gaatgcgcgc cgtcacgcga ttacgacaca
     7501 attcgagggt tttttcaacc aacagttcgg tttccggtcg gggaatcaga acatccggcg
     7561 tcacctttag tttcaagccc ataaattcgg cacttccgag tacatactga ataggttgcc
     7621 cgctcgcccg cttcaaaagg agcgccctga tacgggctaa ctcggccgga gaaagcgggc
     7681 gttcatattt cagatatatt tccagacggt taaggttgag aacttctcgc aaaatccatt
     7741 cggcttcgat ctggggtgat tcgatctgct tacctttcag gtagtctctc gaccacttta
     7801 gcacatctac tattcgccaa atttgccgtt ctctttctgc catagtattt atggaaattt
     7861 aagattgttt tggagccgag caagcagatt ttggggtaaa atagaaaatg tttttagcga
     7921 atcgtcagcg cggaaaactt ttcgtggtgg ttaactcttc ataagctcgg tattgtgagc
     7981 caattgcaga gcggagatca attcatcaat ttcaccttcc ataacttccg tcaaacgata
     8041 aagggtcaaa ttgatgcggt gatcggtaac gcgcccttgc ggaaaattat aagtgcgaat
     8101 tttggcactg cgatcgccgg tactgaccat actgcgccgt ttggcggcga tttcggcttc
     8161 cttttcagct tctttaagcg ccagtaaacg gcttcgcaaa attttcaagg ctttctcttt
     8221 attcttatac tgtgattttt cgtcttgaca cgtgactacc aagccagtcg gcaggtgagt
     8281 tattcgaata gcggtctcga ctttgttcac gtgctgaccg ccagcgccgc tggaacgata
     8341 agtgtcgatg cgcagttcac tggcattaat ttcaatatca acttcctcag cctccggcag
     8401 aaccgccacc gtagccgctg aggtgtgaat acgtccttgg gtttcggtga ccggtacgcg
     8461 ctggacacga tgcacacccg attcaaattt catcgtccca tagacatttt cgccggacaa
     8521 taaaaaaatt acctctttaa agccgcccaa accgttctga gtgccnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnngatta ccaccgcatc tatcaaagca aaaagcaaaa
     8701 tgccaaccat cattacgatg aagataaaac tcgtgccgca ccgcggatga taagttttaa
     8761 atggctgagc attttcgact gttagctctt gcccgctttc aaaggtaaaa acgaccttgt
     8821 gttcagcccc gtggtattga aacagccgct gaatatcttt cataaatgaa atcagccaga
     8881 gatacattag gaaaaaaagg atgcgccagg ctccaacgac aaggttaaaa gccagtgcct
     8941 tgcgctcaat attcaacaac tgggttgtca gccacagcgg taaaaatcca aacagtaaaa
     9001 aggctaatcc aaaggctaaa atcattgaaa tcgtagtggc aattctatcc ttggttttat
     9061 ccgccggttt cggaacattt tccgcctgca tagcaatatt agcagagagc tgaattgttt
     9121 ccagcccaat tttcagcgat tcaaacatac taattatgcc ccgcaggagc ggttttttaa
     9181 aaaatggaat gcggtcggca atggatgtaa aattttctcg tttaatctcg atttcgccgt
     9241 ccggtcggcg caccgccgtg gcataagctc ccggcacgcg catcatcacg ccctcaatca
     9301 ctgcttgccc gccgactaat attttttgtg ccatatacaa ataaaaacga cctcttcact
     9361 gctgtgaaga agtcgttaat cttgttaaaa gaagctaacc tttttgggta tcgcgcttgt
     9421 atttacgatt aaatttttcg atgcgcccgg cactgtcaat caatttctgc ttgccggtaa
     9481 aaaacgggtg gcaggcggag caaatttcta ctttgagatc gccgacagtc gagcgtgtca
     9541 cgaaagtatt accacaagca caagtaacgg tgcattcttt atattctggg tgaatattgg
     9601 gtttcatttt ttaattcctt tgatttcatt cgttaacgct aaaaaagcaa gcaaatttaa
     9661 accgccttgc cttttaattg caaggcattt ttgctaggtg ctcattaatt gattttttat
     9721 ctttttacaa tttggaatgg actgatttct ggcgcttgat actcttcctg caatttgacc
     9781 agatcagccc gttgctgatc cagccgattg ttgaattcag ccggaatggt ttcattccga
     9841 taaaattcgc tgacccgatc cagtttggca atgaatttat caatacgtaa agtgaactgc
     9901 tgttcataag ccgttcgact gtagtctttg ccgagtgcca gtttaaataa gttttgtagg
     9961 tcattatatt gaggcagata gccgactggc gtcgcgatag cgtcatattc gttatgcacg
    10021 cgaccttcag cccataaaat ccagactttt ttatcagtct tttcgttcaa ataattacca
    10081 gaactatctt ttaagaaata attggttgaa aaaacaagtg gcgctttcgc taagcgcgct
    10141 ccgaatttca aatgattctc aatgtaaagt ccgagcggca cgacaacgaa atccaaatta
    10201 gccattggag agaaggtgcg ctgtccttct tgtcccagag tagcggcggt ggtttccgat
    10261 tccagagtcg cacccagcat aacgccatga agccaatcaa gcgcctgaag aatcggcgga
    10321 ttggtatctg aatcgcgtcc gccataaatg atcgctgaaa tgggcacacc gttcggatca
    10381 tcggcgcgcg gatcgagatt ttccagctcg ctgagacgca tagtataacg cgcattttta
    10441 tgagccagcg gaatttcttt cccagccgca tcttttttgc ctttgaacca ttccccggaa
    10501 tgattgatac cgctttccgg cgtctcgcaa cccattccca gccagtaagg ttttccgtct
    10561 tttaccagta catttgaaaa aatcagctcc cgtggcgttg tcaaacaacg gtaaatcaaa
    10621 gcatcatcaa taggattgac atcggcaata atgccaaaaa taccggattc gatattaaca
    10681 gcattgcatt ttttatcatt tgaaacacgc aaataagcta tatcgtctcc gacgatcgtc
    10741 tgaccgggca acatagcggt gctggtcttg ccgcaagcgc tcggaaatgc accagtaaaa
    10801 taggtggtgc gttttttttg tagaggatga actcccataa tgaacatatg ttcagccagc
    10861 caatcttcgt gattggcttt attgattgcc agacggagag ccaacttttt taaacccacg
    10921 ctattaccgg catattgatt attaatcgta aatacgcggt tactttccaa atcaatgtat
    10981 atccgacgct tatcaacatt tttcgaacag ccgcgctcat cgagttctcc cgcactgtga
    11041 ataaaatgaa aaaaccgatc agagccgttt agtcgtcgga attcgctata accctgccga
    11101 tatagtagat cttcactatg agcgacataa gatgagtcgg taatttgtaa agccggaatg
    11161 gaaaagattg acccgagcgg accgagacaa taaaagcgta ccagcatttc tttaccagcc
    11221 atgataccat ctaacaattg atgaatttct tctaagcctt tgacccgatc gagagtatta
    11281 aggcgttcgc tgttcaaatg cccatcatta actaaaatgc gagtatgttc cttgtcacgg
    11341 ccttgatcat tataaccgtc aaaatgaacg gtatgcccgg caattttaag cggctgttct
    11401 tcgccggtac ggagcgccaa ttgtcgcaca taggcaatct cctcaggagt gtcactgagg
    11461 atactaacct tagctggtcg gcaaagtcgg atatactcgg taacaattgc ttcgacatat
    11521 tggttctgga gagcagtcag tttccgccag tcggcagtgt cgagacaatc aggaaatgga
    11581 ttagcatcag atttactatc ttgatcttcg cttttgagag atgttttttg agatttatac
    11641 ataattagaa attataaaat cctttttgaa attaacaaca ataattttag tattttttaa
    11701 tacaatggct aatatcattg tttttaaata attatttctt gaaaaattaa ttctctatta
    11761 caaccgacgg taaaaatccc tggactgtga ttgttttcaa ttgctgacgt cgtactttag
    11821 catccggtag aatccgctca agttcctgcc agaaaagcgg actatgattc ttgatttggg
    11881 tatgggtcaa ttcatgtaga atcacataat caatcaactc atccggcaaa cgcataaggt
    11941 gcaggttgag gttaatatta ttggcggccg agcaactgcc ccagcgtgac ttgaggtttt
    12001 taataaaaag tcggttgtaa cgaaaaccgt accgctgggc taaatcgcgc actcttggag
    12061 gtaaaaattg tttagcctcg atgcgataag cggctgtcag ccccaactta atcgcctctt
    12121 gaatacgtgg attggtaatc ttttgattgg acggatattg aacattaata attccaggct
    12181 ggatgccgac tctaatttca tcaccactag tcggttgcaa tgccaaacgg tgagtacgag
    12241 tttggatggt ttgtttgtct tctatgatga aaataggtgg tttaatttcc ttcagagcct
    12301 gaatattttt taaagcccat tcgcggttgg cttctaggaa ggcgagggca gttttttcac
    12361 tgattgaata cggaacagta agcgtaatct gctgatccgg tttaactttc agagttagtc
    12421 gtcgggcgcg gtggtcgcga cgcagggtaa ctgaaccaag gccatcaatc tggatagttt
    12481 tcacttgagg ttttgaatat aagactttca tcgtaagagt agaatgtaaa aaactttaat
    12541 tgttcaaacc agcccagagt ttcagtgaca gacaagcggg gcgggattta tcccgccccg
    12601 ttcgataacc tagtaatttt actcttagat tttgggcaaa tcagcaagct cagctaggtt
    12661 cttttgcagt gaggcatctg aaaaatggtt gtcttccaat cgcccagcga agtaggaatc
    12721 gtaagcgctc atatcgaagt gaccatgacc actgaagttg aacataatcg tcttggtaac
    12781 accttcctca tcagccttct tggcttcatc aatagtggct ttgatggcat gagcggtttc
    12841 cggtgcgggg atgatacctt cgctccgggc aaaagtaacc gcggctttga acacttcagt
    12901 ttgttgatag gagcgcgctt ctatcaagcc ttcttttacc atcgagctaa ccaatggtga
    12961 agcgccatga tagcgtagcc caccggcgtg gatttttgag ggcatgaaat tatgaccgag
    13021 cgtgtacatc gctaacaatg gcgtcaattt agccgtatcg ccaaaatcat aagaatatag
    13081 acccttggtc aacgacggac aggcagtcgg ttcgaccgcg atagctcgca attttttccc
    13141 ggcaatttta tcccggataa acggaaaaga aaaaccggca aaattactgc cgccgccgac
    13201 gcaaccgata ataatatcag ggtaatcacc ggtctgggct atttgcttct gagtttcttc
    13261 tccgataata gtttgatgga gcataacgtg atttagtact gagccgagcg agtacttggt
    13321 atttttatca ttcatagcaa cttcaaccgc ttcggaaatt gccattccca gactaccggc
    13381 gcaattagga tcattagcga gaatcgctcg tccgctggca gtttcttgac tcggactggc
    13441 gaaaatacga ccgccccagg tctcgatcat agatttacgg tatggtttgg tttcgtaact
    13501 gatgcgcacc ataaatacga cgcattccag accaaagtaa ttgcaggcaa aagccagcgc
    13561 gctaccccat tgtccggcgc cagtttcggt ggtcaagcgt ttgacccctt ctttgtaatt
    13621 gtagtaagcc tgcgcgactg cggtattggg tttgtgactg ccgggcgggc tcacgctctc
    13681 atttttgaaa taaattttgg aatgcgtgcc gatggctttt tccagccgat aagcccgcac
    13741 caacggtgtt ggcctccata gctgataaat ctcgagtacc ggctccggta tcggaataaa
    13801 acgctccgta ctcacttcct gttcgataag agacattgga aagataggtg ccaagtcatc
    13861 gggcgtaata ggtttttggg taccaggatg taaagccggt ggtagagcat agggcaggtc
    13921 agccgcaatg ttataccagg cttttggcat ttcggattca gacaaggtga atttgtgttg
    13981 cttcattttc atttcctttc tttgttattt tttcgttatt gtcaataaaa caaaaaaacc
    14041 cgcctttttt tcgggcgggt ctatgccaat acagagttgc ttaaacgctt atttcagcat
    14101 ctcaccgccc cattactaat ttgccaccac cagttcaggg tattttgttg attttctaat
    14161 gtgctgattt tttccatacg ggcgaatatt tacggatgtt ttggaaaaaa tgcaagtatt
    14221 ttttataatt tacagactga cctagataca caacatccac ttctggtgat tacatttaac
    14281 tttgttgtag tttcagcgaa tcgccaggat aagttcaaat tacccataat aaaaaggtat
    14341 tcaaaggcgt gtttcactat ttttaaaaag ccgattcttg ctctcttttc tggttttcct
    14401 gcggattttt cagcacgatt ctcataacgg ttttttcgtt gggcttgctc attaagacga
    14461 ataactgtcc gcggtgatat tccttcacta tccgcattgc taaactcaga cccaagcccc
    14521 agccgcgttg cttagtgctg tagcctggac ggaaaatatt cttgcggttg ctcttactga
    14581 ttcccttgcc gttatcaatg acatcaacgg caatcttctt cccgccatga aggaggtgta
    14641 attctactct tattttgccg ttctcctgcg atacggcgtc aatagcgttc ttgatcaaat
    14701 tttccagcgc ccaggaaaat aaatccgcat tcaggttgac gatgtaatcg ccagatggga
    14761 taaattccag actgatgccc ggtcccagtt gtggtaaacg acgccgataa tacgaaacgg
    14821 catcgttaat gaccaaatta atgttagtcg gttttagaac cgggcgactg ccgatctgcg
    14881 aaaaacgatt tgccactttt tccaaccgct gaatgtcgcg cgcaatttcg ctgatattgg
    14941 cattgtcacc gatttcatcc ttcagcaatt ccaaccaacc catcagcgcg gtcaacggcg
    15001 tgcctaattg atgcgcggtt tcttttgcca taccaaccca gattgagcgc ttctcatttt
    15061 cccggattaa cgaaaagccg acatatccga gcaaaataaa aagcccgacg atggcaattt
    15121 cgatataggg caagcgccgc agttggcgaa tcatattggt atcaccgaaa tgaatatatt
    15181 gcaatactcg atcttcgtat ttcagtggaa tcggctgatt ttcccgatcc atttcctgca
    15241 ttatcttctt gacttcagcc agagtagcgg gcgaacgatc gttatccgga acgccaatgt
    15301 tgcgccaggc ggttggttcc atagcctcgg catccgagag aatcatcgga gtcgaggttt
    15361 tctggattat ttcttcaaaa ataaaactaa agtcgtcgcc actcatatcc atagcggctt
    15421 tggcataaat atcagcataa aaccgaatca aattggcgga atcctgacgc aggcgcgtga
    15481 cgattttctg ggtatagttc agcaaggcga aaatcataac aattgccaga acaagcagca
    15541 agcgcttgat attgccagaa tgacgataaa tatttatttt catggaatta cgccgataat
    15601 aatcatttta gttgcattgg taaaatcatc ggttgctttt ggcaggtgtt gggcgctttt
    15661 tcttaaggtt tttaatcttc tcccgatttt gataaatcaa cactcccagc aggctaagcc
    15721 agacgatgtc gcaagaaata gtagaaatct gggacgctat ctgataacta cgcggtaaaa
    15781 acttaaattc caccgcataa tttcccgctg gaactggcac tcctcgcaaa acatgattga
    15841 cttgataaat cggtgtttcg acacctgcca cacgagccgt ccaacctttc ggataataag
    15901 catctgccag aaccaagaag gccgggcgct cggtttcgac gtttagcacc acgcgattgg
    15961 gagtataatc ggttacttga acgcttcctc cagtatcaaa tcctgcggaa gtatcaagcg
    16021 ctacactcgt caaagccatt ttagccggat cgaactcggt cgtgttcata aaagctacca
    16081 catccttttc cgacggtaaa aacctgactt cagatacgaa ataagcccgc ggaaggacgc
    16141 cttcatttaa ataaagcact tctttccgtt gctgattaac atccaaaatc gtcaaattag
    16201 cttcattcaa catgccgggc gcaataatat atttggcatt cagcatattg atcacattcc
    16261 agttcaacga gctctgagtc tctcccgcca ataaattgtt atcaattata tcttgaatca
    16321 attgcggctt aatcggtgaa tagcccgtaa cgatttgata gcgataggct aaatcattgc
    16381 tttgaaagtg attacccaat cctagaatcc gaaaaatctc ctgatcctgt ttgagaattt
    16441 tatcgaactg ggtttcgaca aaataacgtc gctcgaggga tttggtatcc actaattcgt
    16501 catctctcag gaaacgcttg ctgacattaa tcatatctac ggcgaccaga ataataatcg
    16561 caccaattag aaaatccggg cgaatctttt tcattttata tagcaccacc agagctccaa
    16621 aacaaattac aaaaaccagc atccgcagag tatccgcctg caaaaattcg cgccgcgccg
    16681 tgcgcaacat ttcaacgact tggggattgt tcataaattg agcgtcctga gcggaagtgt
    16741 acgacagcac tcccggcgcc agcaaaaaga ataaaccgaa caatataaaa aagccgctga
    16801 ctatcagagc cgatttatat ttggctaaat caaatttttc taccagtcct tttaatccat
    16861 aaaccgagag aattactaga atgaaatcaa ccagaatcaa aatcattgat ggaactctaa
    16921 acttgctaaa atagggtaaa tacttgaaaa gtggtttgta taaaattgga aaatgactgc
    16981 cgaaacccag cagtagcgaa aataccagca ggataaataa actgattacc accccatctt
    17041 tgcgataata ccacaagccc agcaaagcca gaatcacgat caaaattccc atatactcac
    17101 atgattgtgt aaacggcata tcgccccaat aacccggaat aggctgaccc cgcagttgcg
    17161 gataagcttt gccggtgtag ggttcctgag atgtgccgcc ataaaaacgc ggtacaatca
    17221 gggtagctaa ttctttgggg ttaagcgacc agcgcgtcgc atattcgaaa gtcacgccgc
    17281 tactctttac ttccgcctga gccgcatctt cctttagatt aatggcttgg ccgccgcgag
    17341 tggaataggg tgtatattca ttagtcacaa ataacggctg ggctgacatt aaaactgagg
    17401 tcaccaatcc ggctacgaat aatccgaggg aaacgtaaat tttctgggct tgtttggtct
    17461 tgacccattc aacaatttgc cgtatgccca gagccataaa aactaatagc gtataaaaga
    17521 ctatctgata atgctgggta cgcaattgga gtgagaaaaa gatcacaaac agtaaaaatg
    17581 acagaaaatt tcgcttcttg actaaatata aaaagccaaa cacggctaac ggcatcgcgc
    17641 aaaccgctcg cactttggca aaatgaccga cggtgatcag cgcctgataa tgtggataaa
    17701 agagaaaggc aatcgttcca agcaaggcat aataccacgg gaattccaac aaccaaaata
    17761 ttaaaagcat tcccaccatc gccaaaataa accagctcaa tttccaatca attaccttac
    17821 taattttact gattaaataa tccagatgga atgaattaga ccagagggca taataaatcg
    17881 gtactccaca aaaaatggcg ggattccaga gtggaatttc accagtttgt tcacgataat
    17941 cattgataaa tttactctgg gcaatgcttg ccatccgatc gccaccagcc ggttcaagac
    18001 gatcaattac atagggctta taaaaaataa ttaatatcaa taatacgata agaaatgcta
    18061 agattgtcgc ctttcgggga ttaatcgtag ctagccagtt aaccggcttc ttattctgcg
    18121 gagggacatt tttggccgat gttgactttt tggttttttt actcataatt caacctccat
    18181 tatttacggt ctaatttaat cttagaacgg ttctttttcc aatttaaagg attacgccat
    18241 ttgcgccagg cgcctttgat ggtttgaatc tcaataggct cgtagaaatt ccagaggtat
    18301 aagataaatg gaaatgatag aattaatagc aattttccag ttagccgaat ccacaaagca
    18361 tagggggata agattagtcc aaatacaatt aataccattc cgcataacat tattttgcca
    18421 attttttcca tctcgtaagg tatgggatag tatttttgtg cagagtggta tattaaaagg
    18481 aaataaatta tctgcgtaac cagtgatgcc agcgctgaac caatataact gaaatatggt
    18541 ataagtaaaa gatttagagc aatattgata atcgaagatg ttattattat tcgtgcgata
    18601 acgggtgttt ttttagttat atttaatcca attagcgaag tgtctttcaa catttcgaaa
    18661 aacaaaccaa acgaaattat cggaattacc ttatacgcat tccagtaggc ttcattttgc
    18721 gccaagaatt tgataatttc ctggccaaag acggaaatcc caataacaca aatcatagtt
    18781 ccgaacgcga aataggtcat agtcttggaa taaagacgtt tattaccggg atcatccatc
    18841 actcgataca taatcggcga aatggcaaga tttaccgaac taatgataaa aaccttgacg
    18901 gtattggcaa tcttataacc tagcgagtag attccaacgt aagagagctc tcctaagaat
    18961 ttcagacaaa atcgatcggc aatacttaat aatattcccg atacagaaga gatcatcaag
    19021 ggcagactaa aacgaatcat ttcctttaaa actcgtattt ccagatgaac ccgtatattt
    19081 ttaatcaaat atcttgatac taaaataaaa taaacgacat agctgataat ctgggcttta
    19141 tatattccat ctatccccat atgtaaactg ctgataaaat atactgtcag aataagactg
    19201 attgtcaatt tggcgacatt gacggaagta tataaaaccg gtttttcctg aactcgcata
    19261 agtgctgaaa ctaagtccat cagaatctgt aaagccgccg caatggacat taatcttatt
    19321 agataattaa aatctgaatt atcgaacaat aaaacagata accgattgga aaaaccgtat
    19381 atcccagcta ccataagtcc tgagataaaa attagagata aaaatgtagt aaaaaatatt
    19441 gacttctgac gatcgctata ttctttttcc cagtaccatc gaaaaaaggc tgtatataaa
    19501 ttcgcactga aaatcgtaac tagcagttgc gaactaactt ccaaaatact cagaattcca
    19561 tattctttta gagagagatg ttgggtataa agcggaacta atataatccc aattaatttc
    19621 gtggaaacgt taccgatact gtaaatagca gaattcttta aagccgattt gacgtaatta
    19681 agcattggaa agtgttacaa taacgagtac acaattaact aaaaattaaa ttaattctaa
    19741 atcagatgct caagttcaat tatttttttt gatcttggtt ttaaatactt cgccggaata
    19801 ccaacatata ttgaccatgg ttcaagattc ttatttacta ggctcattgc acctatagca
    19861 gcaccctctc caattgtaac ttcgggaaat attatacaac ctgccgctat gatcgagtgt
    19921 tttttaaata ctacaggacc tccggttact cgcgtcaaat tcggatttac cattggattg
    19981 gtcataactt caccgctata atcatcagtc tggctgtata ctaaaacacg ccctgaaata
    20041 gtaacgaaat cttcaataca aataccgaat tttccataca aagctgtata ggccgaaata
    20101 tgaacgtacg aaccgattga tatattcccc gagagaatac aaaaatcatc tatcctaaca
    20161 ttactgccta actggatgag ttcgggcgag tagaaactag ctttccggct aattaataca
    20221 ttcttaccat actttttaag tcccaatttt actatttctt cttcattata aaatgatgta
    20281 atcattgcac ttcctcagca atacattaat ataattttta taatctgatc ttggatttct
    20341 tcagccaaat cctcatataa tggtaaacac aaaattctgg atgaaaaatt tctcgaaatc
    20401 ggcatttttt gctttggatg aataaaatga agagtatcta aagaagggta aaagtagcgt
    20461 cgcggaaaaa tttcagcctg attcaaaatt tgttttattt gtaaaaggat tttttcagat
    20521 tcgaataaaa ctggaaaata ggagtaattt tgagtagagt gtggagctat ttgttggagg
    20581 gttaacttat tattatcaat aaatgcttcc aagtaacggt tataaaccac gtgtcttttc
    20641 tcgataatat cttggatgtc atcaagaaca cataatccca ttgcagcttg aaactcattc
    20701 attttagcat taattcctaa agtggcgatt tcatctggtc cgggaatacc aaaatttatt
    20761 aacagccttg ccttttttgc catgtcatcg tctttaagaa caatcgctcc cccctcaata
    20821 gtatgaaaaa tctttgtagc atgaaaacta aatatagaaa cgtccccgaa ttcactaata
    20881 ctcttattgt agtattttac tccaaaagcg tgtgccgcat catatattac ttttagttta
    20941 taagaattag ctatttcttc tatttttcca acattgcaaa cattgccaaa aacgtgaacg
    21001 cctaaaatag ctgatgtatt attattaata tttttctcga tttgtatagg atcaatatta
    21061 aaagtatctg gttcaatgtc agcaaaaata gggatcagtt tttcccagac taaactacta
    21121 gtcgtggcta tgaaagtaaa aggcgtggtt attacttcac cttttaattc taataagtga
    21181 tacgcaattt gtaaagctag cgtcccatta gctgtcaaaa ttacatgagg aacgcctaag
    21241 taatcttcca aacgtttttc tagattctgt actaattccc cattgttggt taaccagccg
    21301 gatttaaaaa tcttattaat atattctaaa tacttttgct tttggggtaa atatgttttc
    21361 gttacgttaa tcatcgttat tttctcttgt tagtatcaat acttagaact tctttccaca
    21421 ttctaacata attttcttca ttgaaatggt ttttgattat tttataatta ttttcagcta
    21481 ttagcggcga gatataagcc agctcctgga agcttacatt ttcaagatct gaatatggaa
    21541 aaatgacaac attaattttt ttgaagtatt cgtacggagt agtttctttt ctgatatata
    21601 cttttttccc aagaaaaagt aaagaaagta tgttgccaag tccttgctga cgcgatgagc
    21661 caaagattgc aacatttact ttgagtaata attttgaata atctttcggt tggataacct
    21721 ctaataacgg cgaaaatttt tctccaaagg catcttgacc atattgaata accgagttta
    21781 tatattcttt tgttccgcca taggataaag ggcaaaatat tttaaaatgt cggtctgaaa
    21841 aacgttttag gtgttcaaga actccaatgt gatcattaga tggatcaccg gaattcccaa
    21901 cctggatggt aatctcttca ttgactaaat cattacatct tatactttct agatactcaa
    21961 agtcagttgg taatggataa acaacatatt gataaatggc tcttgtgtta tatcttgatt
    22021 tggcgatacg ataatctcct tccataaaag caattatgcg aggcaatttt acaataactc
    22081 ggcgacggat agattcataa atggctgcct taaatccttt ttttctagtc tcaacccaat
    22141 atagatcatc accccataat atccagctag ttttcggaat taaagaagga aaaattaacc
    22201 atattattgg ggtaattcca attggtagaa agtgtatata tattatttgt gaccgtaata
    22261 ataaaggaat tagtttaagt atatatttac ttttcgaatc aatgtataaa atatttttaa
    22321 tttgaaatga tggtggatat tcatattgtt ctttatgagg agagcgataa ataaaaaaat
    22381 gctcacttat atcgaaatgc ttatataaaa aattaataaa tttgtaacta taaagacttt
    22441 cgcgagtaaa aatatgaaca gtcagcataa aaatttttca atcattaata tttctctaaa
    22501 tgtcaataga attatataat tctattagtt gttttccctg tatctcggta ttatagattt
    22561 ccaatacggc tttgcgccct ttctcgccca ttgttttccg aagttcggga ttatcctgta
    22621 aatattgtat tttcattgcc aagtcaatag cattatcact tttatgtact aatccacaac
    22681 cagttgtttc gataaggtct ttctgtggtc cacaatcact gactataaca ggccgagcaa
    22741 agagcatata ctgaaatact ttatttgcta tcccggattc atgctgatca ttttttaaaa
    22801 ttggtgagag acatacgtca ctgtcggtaa tatatgatgg catttcacta atatctttcc
    22861 aacgatagtg gattatctga tctttcaacg tgttgtcgga taaatactta ttgaattctt
    22921 ttttttcatt tgtatcaatc ggtcctatca gcaataattt aattttttgt cgcccttgtt
    22981 ggattagaat ccgcaatgcc tcaaaaactg tgaatattcc gcgacgacga gcaatcaccc
    23041 caaaatacag gagggtaaaa ttgtcttcct tatcaagtac tctccgaatt ttatagttaa
    23101 gtaaaccctt aacacaaggt acgtttggat aaacagtgat atcgtcatct ttgagaaatg
    23161 tgtattttct aagtaattgt attcgataat attgacttaa tacgataatt cgatccacat
    23221 tttgaaaata taccgcttcg atatctttcc aatcttgtgg tctgataatt aatcggcccg
    23281 tcaatgtatt cgcccaagta tatgctttca aagctgccgg ataattctca tgaagatcaa
    23341 ctaccacctg aataccaata tagcgtcgag ctttaatacc agatggaacc atatacaaat
    23401 catgtatatg taatacatct atatgattat gaactacaaa ttttttaact ttacgttccc
    23461 aaaagttctt gtaaagattc agagatggat gctgaaatac tcttaaacgt ttaattaaac
    23521 cttttgaagt tggaattcgt tgtattacaa tccctctgta gttttctatt ttagtttgac
    23581 ttctggaata accataacat aaaacataaa cttcatgtcc accttctaat agaataagag
    23641 cttcattttc cactcgttcg tccggtggaa attccttatc cagaatcatt cctattctca
    23701 tgataatcgc tcacaaatgt cagttattat tgttttcata ttctttttaa aattgaaatt
    23761 ccagtgctct ggatgagtca aaagacatat agtagttaag tgtgaacgaa ttgcctcctg
    23821 gggcgtaagc ccatatttcc aattaaaatt attggttata gaactatccg aaatataagc
    23881 atcgaataac atagtaattt ctggatcata ggcttcaaat tggatatcca ggtcggcacg
    23941 atttccatat tttaagatag catggttagg aattcccaat atacgattac gcttatcacc
    24001 atgggatgcg attgtctgaa tatcgccaaa ttgttttttg aagcgagaaa tttcttcttc
    24061 cagcaaatta aaacaggttt tcattacggt atcagtcact tttttggcat ccttaatgtg
    24121 atgcttttta caatatgtgg ctaaagtttc ataatgcaat gaaacttcga aaccatctct
    24181 ccgcatttta tgaacgagtg cctcgcttac agttttccat cggaaatagt aagttgcccg
    24241 agcgccgagc gacttttcga ttgcgtacat tttcgcggcg ccttccgggt ctatatccac
    24301 atcgtgcctc agaattaaca cacgctgtcc attttgtcta taattccgat gccattctat
    24361 caatgatgta agaatatagc cttcatcgcg ggcaaacaac aatagttcac ggtattcatt
    24421 caaacgtgac ttctgacgga tttttttttc gatattaaat ggatttgatg ccattaagcc
    24481 aatgatattt tataaaaata tataccacgt ttttcataat tggaaaccga ttatccagca
    24541 ccatcccgat tctcagtttg cggctattgg aactcattga taatactctt tcggggcaac
    24601 gatatgctta cgataatttt ccgtctcgac aaacatcctc tcccagattg ccagccctaa
    24661 gacgatccac aggaaattat aatgccagcg caggcgcgga tcaggcgggt aattcagaat
    24721 ccgctgaagg tattcgtaat tgaaaattcc ctgatgttgg atgaattcac gcgtcagcac
    24781 tttttcagcc acttttttca aatccttgtt gaattgatcg tagggattga cggcaaagcc
    24841 tcgttttttc ttctgcaaaa tttccggtgg taaatagggt gccattgctt tccgaaaaag
    24901 atattttgtt tgtccgttct tcatttttaa accggctgga atcgtaaagg caaactcgac
    24961 caaatcctta tcaaggaacg gcactcgcaa ttccagcgaa tgcgccattg acatccgatc
    25021 ctcggtcaga atataatcat tcagcatctt ggaatgaaat tcgacatagg caatcgcatc
    25081 cagcggcggt aaatcttgac acgcctcgaa aatcggatcg aagtaagcag gcgtcggcgt
    25141 caagggcatc tgccggaagg acggctggta aatttcctgc ctaaatcttt tatcgtaatc
    25201 ccagcaattg cgcagaatca ggtaacatct ttccagatcg ccagccgaaa acagcatttg
    25261 caagccccgt cggtattcgt cccacttgag aattttagtt gaattctgca aactgaaaag
    25321 cagagagcta atcgaattcc caagcgtttt ttggcagaac cccggacggt tttgcaataa
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gacgaatcca ttcctccaga
    25501 aagataaaca cccagcggca catcgctgat tagctggcgt ctcacggctt gttgtaagtg
    25561 aaaacgaatg ccgtcaatgg cttcggcttc agtcattttg aaattcagat ttaccggcaa
    25621 ttgataatat ttcttgatac ttatctgccc tttttcgtaa atcaaataac tggcgggcgg
    25681 caggcgataa atccctttaa tcaacgtttc atcactttga ttgtaacgta gattgacctg
    25741 gcagtgcaga gcggaataat tcacctgacg gcgcacagcg ggatgcacca gaatcgcttt
    25801 gtattccgaa ctaaacaaca gcacctcgtt ctgaaaccaa tagtggaggg gtttgactcc
    25861 aaaatgatca cgcgccaaaa tgagtcgttc gtctttaata tcatataatg caaaggcaaa
    25921 gatgccgttg agacgcgcga gaaattccag cccatattcc tcgtaactgt gcgccagaat
    25981 ctctgtgtcc gagttggtat aaaaatgatg gccgcgctgg attaaatcag ccttgaattc
    26041 cggaaaattg taaatctcac cattcattat gacgactacc gtgcggtctt cattataaat
    26101 cggctggtcg ccagtttcta aatcaatgat tgccagtcgt cggtgtccga gactgacatt
    26161 accgtcgtgg aagtacccgg ctttttccgg accgcgatgc tttaataaat cagtcatctc
    26221 ggtaatcagc cgatggttgt tttcatttgg atcaactatt ccgcagatgc cgcacataat
    26281 ttataatttt gctaaaagtt catcgtaaaa ttgttatcat attccgtccg gcaataagga
    26341 aatagccgag cgaaaacaat tctttcaaaa ttaataaaca ctcgcgatca cctggcatat
    26401 tttctcgata ttatcccttg acagttcagc atacattggc aaagatagaa actgggtgga
    26461 tagcagttcg gaatttggta aatcgcctgg tttataatgc aaataactat aagccggttg
    26521 taaatgcagc ggtaatggat aatgaatacc gaaagctatt tgattggctt ttaaagcctc
    26581 aattattctt tgacgccgat cagtggcgat agcaaaaatg tgatagacat gttttttggc
    26641 cgggttcatt tccggtaagg tgattggcag attttttaat aacgaatggt aggtattggc
    26701 aatgttgatt cgttgctcag tccattctgg caggtggcgg agtttgacat ccagaatagc
    26761 cgcctgcaaa gtatcgagtc ggtagttata tccttcagtc tggtggacgt atttttcttt
    26821 gcgcccatga ttagccagga gcttcgcctt ttcgtaaaga gtttcatcgt tagtacccaa
    26881 cgctccggca tcaccataag cacctaaatt ttttccaggg taaaagctaa aagcggctac
    26941 atcgccgaaa gtaccgactt tctgaccttg aaattctgcc agatgggctt gagcgcaatc
    27001 ttcgatgatt ttcaaattat atttcttggc aaagctgaca attcggtcca tttcgcaggg
    27061 attaccgtgc agatgaacca caacaatggc tttagtctta ggcgttatca gcttttctag
    27121 accgttgcaa tcaatgttaa acgaaaccgg atcaatatcg gcaaaaacag gcttagcgcc
    27181 cacctgacgc acagtttcag ccgtagcaat gaaagtcata gcaggaacaa tcacttcatc
    27241 gccggaagtt atgccaagaa tttcatacgc cagatgcaga gccgctgttc cactcgctaa
    27301 gcctagcata tatttcaggt tctgggctgt agcaaaattc ttttcgaagg tggctacctc
    27361 aggaccatta atgaatttgt aactatgaat aacgttgaaa atggctgaat ctatttcctc
    27421 tcgaatatta agatattgcg tttctagatc cataaaagga atcatataga acctctttga
    27481 ttataaatcc ctgattttgc gggcgggatt gccagcgtaa attcctgagt gtaaaatatc
    27541 tttggttacg actgagccgg ctccaattac gactcggtcg gtaattttca ccggcaaaat
    27601 ggtggcatta gtaccgatgg cgacgtgatt accgataatg gtcggtttcc ataactcccg
    27661 attaccacca gctggtttac ctgatttaaa tgtgtcatta acaaatttag ctccatgaga
    27721 aatgaaacaa tcatcgccga tcgttactaa ttcgcaaata aacgaatggg actgaatttt
    27781 acagcgccga ccaattgtga cacctttctg gatttcaacg aaaggaccaa tgaaagtgtt
    27841 atcaccaatc tcacattcat agagattaca cggctcaata ataactacat tcgtcccaca
    27901 tttaatatca cgaatcccga ttttttttat aattggttca gccatattta cttcaattta
    27961 ctttgcgaat aaatgtcttc aatgattcga acgactttca gtccttcaat ggcgttagta
    28021 gcgatggtat ctttgccatt taatacatta acgacatttt cgtaaacttt atcatgattc
    28081 gacatcgagc cttggtaaaa tccatagtca ttggctttat tgaccggcgt aacgttctgg
    28141 aaagaataat cttcgatatt ttgatattca agtgcgttta aatattgacc gccgattttc
    28201 actgtcccct tactaccgaa cacggttatg gaaccttcca tatttttttg gtagctattt
    28261 acagtgtaat taatagtccc gatggctcca ttgaaaaatt cgattaaaac ggcgccggta
    28321 tcttcaaatt caatgttggg atggagataa ttctgaagaa gcacttttac attcttaaaa
    28381 tcgccgaata accagactaa tagatcaatg aaatgactga attgggtata aagggtgcca
    28441 ccgtctttat cgagccgtcc tttccagtcg gattgtaaat aataatcctc atttctattc
    28501 cagaaacagt ttaattggac ataaaaaatc ttacctaacc gattttcttc gatgactttt
    28561 tttaattcca caacgggagg attatagcga ttttgtttga caatgaagag acgtcggttg
    28621 gtttgttcag ctgtataaat catattttca cagtcgcgca ctgaaagcgc cataggtttc
    28681 tcgcagagaa catgttttcc ggctttcaga ctttttatgg aatgttcggc atgcagatag
    28741 ttgggcgtac acaccgaaac gacatccaca tcgggttcat tgcttaagag cgtatcaatg
    28801 ttgaaataag ggcggcaatt atatttctcc gataatgcta atgccgcact ttctttaata
    28861 tcgcagacgg cgcttaactc gcccagacga tgaatttgtt cggcgtggcg ttgcgcaatt
    28921 cgaccacatc ctattaacgc aaattttatt gtcttcaatt atcaaatccc ttttnnnnnn
    28981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttatat agtaagtttt gccagtaatg
    29101 gcaacttcag acatattgcc agcattggtt ttatccttaa ttaactcttt caaaaagcac
    29161 atcacgcata tatcgaaaat tagtgaaaat tggctgagaa tcaattgaaa agataaaatg
    29221 attcgattca ggattttctg taatatttgt caaaaatttc agccagttgc cgcgtaaggt
    29281 tgcggcgggt aaaagtcatt acatattcct tatctatttc aacagccggt tcaccttttt
    29341 cccatttttc atataaactc aataaataat tagcagaatc cgcgacgtta tcatattcaa
    29401 acattctacc ggcattggct ctgtgtagga ttttcgcggc atcgccttga gtcggtccat
    29461 aagccagaat cggtttgtac gtagcgagat aatcatacat tttataattg attactccgc
    29521 cttcgggagt cacgtgaata aacagtaaaa gtacatctga agaaacaagg tatcgcttga
    29581 cttcgtcggc tggcatataa ggcaagaaag tgatcatttc gaaaacggaa tattgccgca
    29641 gtttctgttt tacgaattcg gggaaattgc cgataaactg aattgtaata ttttctctga
    29701 tattttcatt ttgaaccaaa acttcaatgg ctttacaaaa tgcctgaata tcctgttcaa
    29761 tactgatgct tccggcataa gtgatagtcc attttgcatt tttgcgcggg acaaagtcaa
    29821 catcaagtgg ttcaaaacca ttatagatta ggtaaaccgg tttattaact ttcttttgaa
    29881 ataaattgac taacgaagga ccgattgtgg taatagcggc cattgaattg agagtccgtt
    29941 tttcaatctt ttgatccaga ttgtgaatcc atttccagcg ttgggaattg gtctgtaacc
    30001 aatagacatc cgtccacggg tctcgcatat cgccgacgca cgggatacct gtttttcgtg
    30061 ataattcaac tgcaatgcag tgattcgtct gaggtggaga tgaagaaaag attagcttaa
    30121 tatcattttc tctaatgatc tgtaaacctt ttttaatcgc gaatcttttc cataagaaac
    30181 gcgtatcggg aatagcgtta tattttaccc agcgtgatag ctttcctttt aggcctttat
    30241 tgtttttctc tgtaaaagca aatggaacga acatttcttt ggaattcttc gcgactattg
    30301 agaaaatccg atgtaaagag atattcttag ccctatagat ctttgtatcc ggtctgactt
    30361 ctttcaatgt ttgaggatca ataaatggga aatcgccatt ctcgacagtc aaaacgatcg
    30421 gctcccaacc gaattcaggt agatacttgt aaaacttgag gattcttgtc gtggtcgcgc
    30481 ctcctgctgg tggccagtaa taagaaatga acagaacctt tttcattatt tatatgcttt
    30541 tattaccaga tgccagcccc agagatggcc aagtgcgcga tggactgctt tgggaatcaa
    30601 atcccaaaca attccatatc cggtggttaa taaatattca gtctcgattt tcacaacttt
    30661 aaaattttta aacattgcga atatttgagc cttggtatag gttctttcaa tcggacagcg
    30721 atctttacga ttaccgtcaa aaggctgatt aatcagacga tgaatgtaat aattgagcga
    30781 tttacgatta tacagcataa tcaccgccaa accaccggga gccaataccc gccagacctc
    30841 atcaatggct ttttgggtat cgggagtatg atgtaaaact ccgaaagaat agacaacatc
    30901 gaaagtatta tccggaaagg agagattctc cgcatcatct actttcagct cagctggcat
    30961 ctgatatagt tcaaagcgtt tacgggctaa attaattcct tcttccgtca ggtcaatccc
    31021 ggttactcgc attccgcgcc tggcaagttg caataaatca gttcccatac tacagccaac
    31081 ttcaaggagt gaaccatttg gtttctcttt agcgatccaa tcgaataatt caggtagata
    31141 gaagtgatat ttatatcgaa cacgttcggc ttcctgataa aattcttccg tcccgcgctc
    31201 accagtcttg agaaattcag tctgacaaac attccgattc cagaattcct caacgtcttt
    31261 tttagttggt tgttgctcta ttttcggcat aatatttcct gattatttca ctcattgcta
    31321 ttgccatcca gccattactc caatgcataa tagtaaatgg ttttttaaaa aatcttcctt
    31381 tttggtaata aaaaaaacct tcttgcttat tttgcatatt attgatagcc cactcggcga
    31441 ttcggtaagc aaattcttca ttttggcgat taaaccggcc agcttttaga aaagtaatga
    31501 tcccctgagc cgaaccgtgg acgtcaattg gataaatttt gttattcatc catttaggtg
    31561 ttccgtcagc caagaacaaa ttctctaaat aatattgcag accgcgatga taatttccaa
    31621 gaaacgaatc atccccgcta aaatctatat aatccagaat tgcatccaat atatatccgg
    31681 tatggtaatt gtcgtgcatc tttaaacgat tggcagtcgg tggatgagta taaaaccagg
    31741 cgccatagtc ggtttgctta tcaacgacgt aactgattaa tttacgggct tcttcgatca
    31801 aggatttatc attggtaaaa tgggctaacc gagcacaaaa tgctccacat aaagcactca
    31861 catccattac aacccaattg atgctttcat ccggcacata gcttaaacac ttcatcttgt
    31921 cgtcttcata taagaccttc ggagcttgga ttaaaaaatc ttttactcct atcgcataat
    31981 ttaaatataa attctttccg gtaatttcat aagcatttag gaaagccgtc ccgacaaaat
    32041 acgacaccac gcggttaggc aaattaggtg gtgcgaaaaa gccgacatct tgccagggat
    32101 attggtaccc ccaagaattg cccggaaatc cggttgattg attttggact aaccaatcca
    32161 gtaattcctc tgctttttga agaaatttct cggactttct tgctaagtat agatttaaat
    32221 aggtcattgc aaaaagggcg attcctttcg gatttctggt gcgcggaatg gcaaataaag
    32281 gccgtaaatt tagcggtgaa cgcataatcg cttggctata aaataatctc aaatttttat
    32341 tgccgaatga taatggcaat aaaatcggac tcgataaagc gtcgtattta ttgaatccgg
    32401 cataatcatt tctctgtgaa taggaaagca cagcgtccaa tgagtcattt actgtcttta
    32461 taacttgttc taagctttta ttatttgttt ccattcagat aatatcttta atctttaatg
    32521 attctgatat ttatttcatc tggctgagat tgacatacta ccattaattt tttgcgtttg
    32581 gttgaagtaa atatactgcg ccgaggttaa aaatctctcg aacaattggt aaatcacagc
    32641 gatgtcctat gtaaggaata ccataattat atttaaaacg gggacgtata aggaaaaaat
    32701 cctttttcag aattcgtagt ccattttcat aaaccatttt ttcaaattgt tttattgaaa
    32761 gccgagaatg tttggttgcc aacatattcg atataatgtc agcgcgaatt ccctgcgatt
    32821 taagaatcgc ggaataaaat ttatccggca ataaaaagag atagggaatc cgatgtagcc
    32881 aacccggtag cccctgctga tgtcccgcaa aaggcgaata cttcggtgga aaggaaatat
    32941 aaatgcgacc gcctttttgc aataaatccc gcatattttg gagtgccgcc gccttttttc
    33001 cattggcaat atgttcaatc acatcaccgc atatgatcaa gtccatttta tcctgaattt
    33061 gattctcgta ggaacttgct tccgtaatat caccgtgaat aaagataatc ttagaagact
    33121 tatttaactc ccgggcgatt tctaagcgcc cgcgcgaata ctccaaacca taacattgat
    33181 tgttcttagt cgcaaaataa tttaacccac cgccttccgc cgaaccaact tcaagaattc
    33241 tgagtcctga aacaggagaa atacttatat atttttctaa aaaattggcc gctgtccagt
    33301 attgttcttt atggaaattg agatttcggg ggggagtatt tgtcactgat aatatctccg
    33361 cacgaacccg gtaacttcgc cgcggcggaa gattttccgg cgaacataat tataagtaaa
    33421 tccataacca taagcaaaaa tctggcaaac tgcaaaaata ggcgccagcc agccgacttt
    33481 cacggattta tatttaatcg agctgtcaat gaaaatgtac ccacagatta gcagtgccag
    33541 aacggtaatt gcaacccata ttttccaggc taaaccagtc agtaaggtaa atatcgtcaa
    33601 taatgtatag ccgaccgcgg ctatcgccgg aataaaatgc aacggttcta aattgcccgc
    33661 atccagataa ctcagattga tacgcgccac tccccagtta aacatctgac gaaagaattt
    33721 gcgcaggcta gtgcgccgtt tatgataaac tatggcgttc ggtatataaa taaccttagc
    33781 gccgctggct acaattcggc gactgaattc aatatcttga ccgtgccgta ggttacccat
    33841 accacctaat ttttcataaa tctcacgtcg gatgcccata ttgaaactgc gcgggtaaaa
    33901 ttttgccagc cgtttttgcg agtgaccacg cattccaccg gtggtaagga aagatgtcat
    33961 actgtagtca attgccttct gcaatggcgt aaaggaatcc agcaccgagt ctggaccacc
    34021 aaatgcgtcc ggcgcttctt tcgtaatagc ctcatcaatt tctttcagcc agttggcatc
    34081 cgcctggcag tctgaatcta taaagaggaa atactcgcct tccgcctgac tcatcccaaa
    34141 gttccgcgcc ggaccgggac cttcatgatt gcgtcgaaaa aacctcaggc gaaaatcaac
    34201 ctgcgatgcc aattcattaa ctttttgttc cgtttcgtcc gtcgaaccat cgtcaatcac
    34261 cagtacttcg aaacgactat gctcgcaagt ctgggcttgt aacgatttca gtaaatcaat
    34321 tacttcgtcc gctcgattat atgacggaat aatgacggaa aacctatatt tgggcattat
    34381 ttttctttcc tgttctggaa agtaatcatc tcgctaatca agccaagtga aaaaaactgc
    34441 ataccgaaaa caatcgccag caccccaaaa atcagcaaag ctacgtgatc tttgaatggc
    34501 tcctgaaatc cgaattttag gatcaaaacc cataattccg atagcaagcc aatcaatata
    34561 attactattc cccataatcc gaagaagtgc atcggtcggc gcataaattt agtaataaag
    34621 atcaccgtta ataaatcgag gaaaccttta taaaaccgac tgacaccgta ttttgttttg
    34681 ccatatttac gtggaaaatg tttgacttta tactcgccgc tggtgaatcc ctcaaattta
    34741 gccaataccg ggatgaaccg atgcaattcg ccatacagat atatggattt gacgacctcg
    34801 gcacggtaaa cttttagacc attattgata tcgtgcagtt tcacaccagt taacaagcga
    34861 gtgactgaat taaaaatttt cgaggttgtt tttttaataa atttgtcctg gcgcttttgt
    34921 ttccagccag ataccaaatc atagcctgat tcctggagat aatccagcat ggccggaatg
    34981 gcttccggat catcctgcaa atcggcgtct atagtagcca cgaagtcacc ttttgctaga
    35041 cggaaaccct cattcagggc ggcggcttta ccacgattac gttgaaaacc ggtaaccgtt
    35101 acattgggaa aagtgctggc gatttccctg gcaacttgtg ccgagccgtc gcgactgccg
    35161 tcgtcaacaa tgattatttc caggttatag ttcgttgaac ggaaagcggc ttgaattttt
    35221 tcgttcagtt cgcgcagtga ttccacttca ttgaaaaccg ggatcactac cgtaacaagt
    35281 tttttttcat tctccgaatt cacatttacc tcgataatgg cgaatatatt acgtccgaaa
    35341 tttaagcatc tcggcattga aatgggagaa atgaatttct attgcgatct cttccgcctt
    35401 gtttttgata actatttgag cgggaatttt taacctagca acatcagcat attctttata
    35461 aacaataatt atctgtttgt ttttgtaaag ccaattaagt tcagctggtt caccctgacc
    35521 gggtttaaat ttataataca aaacaggcgt ttcatcactt gcctcattga actgagaagc
    35581 ggtttcgttg gtggtagatt caaaaggcac ggctagtaaa attcgtcgga ttaatgccgg
    35641 agtgattgga ccgcaagaaa ttggtaattg gtcagtttct aattgcgttc cgctaaaata
    35701 cctaccatcc cggattaaat gtaaatgata agtatcaccg cctaattcaa gattagccaa
    35761 cttccttccg atcgggtctt tgatccaaag attcaatgta tccgcggttc ggtactcaaa
    35821 atcaaaatag attattccgc taccagccgt tccactaaaa ctcgcctggc cagaaccgga
    35881 aagattagcc agctgtttca attgccgact gcgttctctc aaggcgggcg cccaatgctc
    35941 atcaactttt tcatgtttga ggggggctcg actacaattc cagccgctaa acgataagat
    36001 caggctaatt agtaaacctg tgaacttacg gttgcagttt acgcttaagg ttttcattat
    36061 ttttatcaat ttcggcggct cgttggtagt aaatacgggc ttttttaggt tgccgtaagg
    36121 cttgatagac gtcgccaaga tgttccagta cctcggcatt gtcctgctca atttcgagag
    36181 atttctgaat atactttatt gcttctttat attttttctg ccggtagaga atccagcctt
    36241 tggtatccag ataagaggca ttattgggct ctttagccag agccaaatcc accattttta
    36301 aggcttcgtc gagtcgttca ccacgcactg ccagactgta agcataattg ttcaaggcga
    36361 tgacgtcatc gggattatgg cgtataatta cttcataaag gctatcacac tgcgaatatt
    36421 tttttaacat atcccagatt gccgccagca gatgttgagc ttggacatta tttggttcaa
    36481 tctgtaaagt cttcagcaga accttttctg ccgattgata atcgcgcagg gtattgtaaa
    36541 tcgaaccctg agcgagtaaa aacgatggat cattcgggaa acggattctg gcagaatcga
    36601 gaatatcaag agcctgttga taattttcat tcctaacata aagtaatgcc agattggcgt
    36661 aactggcttt gcgctcagga ttagcatcga taattgcttc gaggattgtt tgagcttcct
    36721 caaaatggtc gctccagcac aaaatttctc cgagtcgttc tcttaattca atatcgccgg
    36781 gatttaaaat aatcaatttt ttgtagatgt caatcgctgt ttcaaagtca ttgcccaaag
    36841 ctgctaaatc cgctcgggtt tttaaataca ataagctatc gggaaatagt tttaccaatt
    36901 gatcataata agcggtggct ttatttagat cgcgattgag aatagcgatc tccgccgcct
    36961 tctggagggc ttgatattga gtcgaatcaa ctttcacaat ttcatcgtag agttgcaaag
    37021 cctgatcaat ttttcggttc ttgagataaa tagcggctaa ttgatacatt atttccggtt
    37081 cattgggtcg taaagtcata agagcctgat actgtttttc agcctgttga aaacgcccgg
    37141 tcagaaaata aatctgggct aagatttcgc gagtttcggc gtcatccgga tccaattggc
    37201 aggctttttc cagagccgcc aagccacgtt ccagtttgcc gagattaata taggctttgc
    37261 cgagagagct gtagatagcc gccgatttag tttcgtaaag aagtgcatcc tggtattcca
    37321 gtactgccat agcataatcg ccctgtagag cataattctc accattcata aaatgcgtca
    37381 gagcctgaga attatattga gtattttgag atgattcagc cggcttttcc gcagatttac
    37441 tggtttgttt ggtagcggca caactaacca gagcggctaa tacaagcaaa ccgactattt
    37501 tcctgagaat agttgtcgcc ctgcttggat atagtttttt catgtttttc acttcccact
    37561 ctaactaagc cagcccaaaa aacggggctt gacaagagag tggtcttatg gttattgata
    37621 aatttgatcg cggttgcatt tgcaaacccc gacaatctgc tccttaaaac gcattgatct
    37681 tttattggac ggatcggaag tagtctcaat atgttttaca tattcaacgc tttccccaca
    37741 aaccggacaa taagttttat tatcttttga ggatttggct actttatcgg caaatccttt
    37801 gacaccttta ctcataattg actcctttta agttgcatat tctttcttaa ataaggttac
    37861 tttattgcca cccagacgtt ttgattgata cattgctaaa tcggcatgcg aaatcatagt
    37921 cggaacatca gtgccgtcca tcggatattc cgccataccg atactaattg ttatatgttc
    37981 gcgaattccg tctttttcaa atacaaaact ctggatatta ctgcgaattc tttcagccga
    38041 tttataactg ttatttttat cggcattaat tagaattacg gcgaactctt caccaccata
    38101 tcgcgcgact gtatcaactg tccggacact tccgcgaata atagaggctg tttttttcaa
    38161 aaccatatcc ccgaagatat gcccgtacgt gtcattgatt cttttgaact tatctaaatc
    38221 gagaatcatc agggtcaggg aattttcata acgacgactg cgctcgattt caatctggag
    38281 acgttcttcg aaagctcgac gattatagag accggtaagc ccatcaatca tcgctaaatc
    38341 tttcaccgtc tgataagtat tcatccgatt gattatttcg ctgaggtttt tagcaaataa
    38401 aaccagggta tcgctttcat cccgtagata attgttgggc tgatagcttt ccatcaaaat
    38461 gccacccagt cgcttgcgcc ctacttccaa cggcactccc agacaggaac gaaagggcag
    38521 aatacctaaa tctcccggtt gaaaacgaaa tgataatttt tcctgctcat aatcgtttag
    38581 caggagtatc tcaccgttct tgatgatttt ctcccagagg ccgcctttcc aggaaatacg
    38641 atgaccgata ctatagtcag tatcgtaacc ttcgacatac tcaatataga gttcgggtga
    38701 ttcctcctca ggtaaagtgg cgaaagtcaa tttatcaaaa ttaaaaaatt tgtgtaaaac
    38761 ctggctggct tctttgtata attcaagact ctcaattcca ataggtaatt ccgtgttcaa
    38821 gcggcaaagg gctttgaaaa aggcaatttg agtgttgagt cggtcaatgg cgtcaatttg
    38881 ccagattgag gaggaaaata attccgcaaa gagtttcacc agatcaacat cttcgtgccc
    38941 ccaggcatta cggtcaatgc tatcaagtcc aaggacaccg acaaatactt cctgattgaa
    39001 cattatggga acgaccatga tcgtgccgac tttgggcgga acagtataat aaaccaaact
    39061 accttcttta agaccatcgg ctgagtatat ctgtggtttt aaagtcagat gcaattcccg
    39121 gaaaatagtg ccttcagcct tgaacttggg acgaaactca attccttctt gagactgata
    39181 agcctgcaaa atataagtat gttcatcggc attaaataaa tagaggaatg cactacgcgc
    39241 cattaaagaa gattgggcta tcttcaatat ttgggcgata atttcgttgt aattctgatc
    39301 cggatcaatt ttcagcttca gactaagctc accagcaaat tcgtcatccc ggctgatgga
    39361 agatcggcgg gagtccagag ttttctcggc taaatcacag cgactcnnnn nnnnnnnnnn
    39421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39481 nnnnnnnnnn nnnnnnnnnn nnnnnnaccg ttttaatctg atcctcataa tttggctcat
    39541 attcaaacgt gggttcagct ggttggctcg aagaattatt cgaagtagca gtatcgctcg
    39601 gaatcgggtg attttgttta gaggtatatt gaggagactg taaaccggat tggttcggat
    39661 tattcggcgt cacatcttga ggctgttgag ctggagattg ggtcattttt tgacgtacga
    39721 tcggcggcat attgtccggc gaattttcct gactctggga ttgcagaata aaaaacccga
    39781 ctaccacggc aaccactgct acggcaaagc cgtaagcaaa tgacgggatt cggcttaagt
    39841 taaaaccctt atgtgagtta ggctgagtag ccgcactgcg atcggaaaga atccggttgc
    39901 gtaggttaag tttgaaatcg tcacttacag aaattttagg taagcttaac atattttgcc
    39961 gggtcgccaa gatggagcta taaattttct ggcaaactgg gcattcattc aggtgctctt
    40021 ggaaagcttg acggtttttg aaggagatgt tttcgtcaat aaaatttgat atggaattcc
    40081 taaattggta gcaattcatc ataaattttt gatttccccc attttttact aatcatcttc
    40141 tctatcgcct taaatgttca agttcttcct gcaatctcag ccgcgcccgg ttgatccgtg
    40201 acttgaccgt ccctaatggc gcgtcaatga tattgctaat ttcctcatat gaaagatctt
    40261 gaatatccct taaaatgatt acggtacgaa aatgttccgg taattttata attgcccgct
    40321 gaattatttg ttcctcgatc tcgcctgcca gtttagtatc cggcggacgt tcggtggagg
    40381 ggtactcgat ttctctctct ttatcttctg aagctgtacg cgaaaaggga aacattttgc
    40441 gattcttgcg cttgcgcaga atggttttag ccaaattgcc agtaatagtg taaatccagg
    40501 tggaaaattt ggctatttca cgataggcat ggcggttttg ataaacttta aggaaagtat
    40561 cctggacaat atcctccgct tcttcccgat ccaggacgaa acgataggca aaattcaaca
    40621 aacggtcggc ataacggttg acaatttctt cgaaagcgcc ttcatcaccg gcttgaaaac
    40681 ggctgatcaa ttcttcatcg gtcagttgaa attcggggtt catttctgtt tttttcttag
    40741 gagatgattt catacaacaa ttgaattaat aaatcttctt tattcaaatt taaagatttt
    40801 gattgtaaat ccacatttct aatacaggta aaaattgcac caaaatcagc ctgtttatag
    40861 ttctttattc ccagctcata acaattactt ttcttccaga atttttcttt ttgaccatgt
    40921 ccctcataat cccaaatgcc gctgaataag tcatacaatt gggtggtaaa ataggggatt
    40981 gatgcatcag cttggattag agccaaaccg atctttattg ctttggttat gtcgcggtga
    41041 gctacagctt caaggaaatt aatcatatcg taattcttat cgccgccaac aattagacga
    41101 acatcatctt cttcgatcat tttccgctcg ccaatgtaaa tcagcaactt ttccaattca
    41161 ccgatcgtat ggctgagcgc atcatcggtc aattctatca gcaatttgag cgcttccggt
    41221 cggatggaat attgactttt ttggaggtgg cgtcggatga attcatcaaa cctggagggt
    41281 ttgggcgtcc aagtcgaaat cgttttgacc ttccgcgatt ctttacgcaa ttgttcaatt
    41341 aggctggaat gactttcgcc atcggcagtc ataattaaaa cagtatcctg tccggggttt
    41401 tcgataaaat taaaaagccg agcacgcaga tttggctcaa gattaccaat atttttatag
    41461 ataataatcc ggcgcctggc aaaaaatccc atactgaaaa gattatcaat gaattcgaga
    41521 tcgctatcaa caccgccgta gtaaatcagt ttttccggcc tctcagattc ttgtttgcca
    41581 aaagcctgac aaatggtatt gatggcttcg tcctccagat aaagatcacc accccgtaat
    41641 aaataaaccg catcgagatg accttgctca attctcgcta attcacttaa gaaatcactc
    41701 ttcgacattg ccattggatt ccttgggctt cttattagtt aaacttttat tcgaggaaaa
    41761 atacaactgt cagtccgact attatgcaat agataccaaa ccagctgaat ttatcacttt
    41821 gcactactct tagtaaaaat cgaatagcaa tatatccaac aataaaagag gttaaaaatc
    41881 caaggattaa tatcccaatc atattgggat tgagagccat accaatcaga tctttgagat
    41941 gcaaaaccaa tgcgcccaga attgccggca ccgagagcag aaacgaaaaa cgggtggctt
    42001 ccatccgatc caagccacac cacaaaccgc cgctgatcgt cgaaccgcta cgcgaaatac
    42061 cgggcaaaat cgctaccgcc tgggcacaac caatcaagag acttttacca aaagtcaatc
    42121 ggcggccttc ggtttttacc caccgcgttg ataaaagaat caaaccggta atcgtcaacg
    42181 taatgccgac taaaaatacg ctatggaaaa tttgctcgaa gaagtcgtcc agtgtcaagc
    42241 cgaatattac cgccggaata gtagccacaa ttacaaaaat gctcagccga aaataagaat
    42301 cattctgaaa cggttgcgct ttgcgtagcc ggaaaattcc acgacaaaaa cttactatca
    42361 tccgccatac gtcattgtaa taaaccgcca gcacactcaa taaagtgccg gcgtgaacca
    42421 taacttcaaa ggcaatattg tcggtcgcta acccaaagag atgctgaacg attaccaaat
    42481 gtcctgaact gctaatcggc aggaactccg tcagcccctg tacaattccc aaaataaaag
    42541 attcaaagta attcataaac ctccaaatat agtaaattta ttccgagcta gccggtttac
    42601 caagattgtt ttaccaatcc ataatgacta aatatctttt aatctcgacc aaaaattatt
    42661 taaatcaatt tgagccaaat caaatgaatg taacatttca ccaaggctga tcggtggaag
    42721 tagcatttgt taacctgaag aaggtcggtt tggtaaattg gttatcagac cttctgaagg
    42781 ttgcaatctt tctcgttatg cctgatagta gggattaaat tgacagtatc aatttatgcg
    42841 cacaacctaa aggctggact gttcacctcg ctctatcctg aggcgctccg acattatcgg
    42901 agcattccaa attactttca agagaaaatt aaatacctta agtcttagag cttgcgtggt
    42961 ggttttcaat ctatacgaat ctagcaatta ttcgattcgt ttgagtgtct cgaccaacag
    43021 ccgccagaat ttctcgacag tcttgatttc gacgcgttcg tctggcgaat gtgccccctc
    43081 gatagttgga ccaaatgaaa tcatatccat acccggtgaa gtagccccga taataccaca
    43141 ttccagtccg gcgtgaatag ccttgacctg gggttcaacg ccaaaaagat cggcataaac
    43201 ctttttcatc aatgttagaa tcttcgaatc catattagga gtccaagacg gataacctcc
    43261 actttgaact acttcggcgt tggctaaaag accacaagct ttgactttat cagctaagcc
    43321 ctcgatttcg gaggcaaccg aacttctctg actggtaaga actctaatct gcttatcact
    43381 agtactgact atagcggcat tattggaggt ctgcaccaat cccttgattg tggggctcat
    43441 tctcttgaca ccgtgcggga tggcatagag gcagttcaat actcgcgctt gaaattcctt
    43501 cgtgaaaacc tgcttcgggt attcatttaa attttcaacc gtcagtttaa tgccacgttc
    43561 aacgtcttta tactcgttat ttaatatttt ttcaaatttg gcaatctcgg tctcgaaatt
    43621 agctaaatca gccggtttta tcagcaattt ataaacggct tcgcgcggaa tggcattatg
    43681 tttactgccg ccttccaagt aataaagccg aaagtcatat ttctcagcgg ctttccaaac
    43741 tatgcggttc aattgcttga tagcattggc acgcccctgg tcaatatcca gtccggaatg
    43801 accgcctttc agcccggaaa tttttagggc aacattagcc atttgttgag gcgctttttc
    43861 ccagttaata tcgaaaaata gatcggtttg ttttccaccg gcacaaccga catacaaagc
    43921 gccatcttct tccgaatcac aattcagtag gattttgccc ttaataaatc caggctttaa
    43981 ggagtttgcg cccgttaagc cggtttcctc gtcaatggta aaaagagctt cgagcggcgg
    44041 gtgtgctaaa gttttatcct caagaatcgc cagggcggcc gctacaccga tgccattatc
    44101 ggcaccgaga gtagtaccat tagcccttat atactcgccg tcacgaacca attctatcgg
    44161 gtcttttcgg aaatcatgga cagtgccgct gttcttctca caaaccatat caacatgccc
    44221 ctgtataact accggcgtgc ggttttcata accgggagag gccggtttag aaatgacaat
    44281 attaccgaca tcatctttgc gatagggaag attcaatttt ttagcaactg attcgagcca
    44341 ggcaattacg gcttcctcat gtttggattc gcgcggaatc tggctcattt cagaaaaata
    44401 gcgccataga ctggccggtt ttaatccttc gattgcttct ttcaaataaa cctcctgcta
    44461 gtttttaact cttatgagtt tagttttttc agatattatt gagtcaaaac ttcttgcaat
    44521 attgcgatcg cctggtcgag ttcacttcgg gtaatattta gcggcggcgc taggcgaatg
    44581 acgttttcat gagtctcttt tgccagaatg ccacgctcct gcaacgccac acagaagtgg
    44641 cgagcgcctc cggctgatgg tttcaaaacg atgccaaccc ataaaccttt gcctctaatt
    44701 tgttcgatgt agggactctc gatttcattt aatttttgca ttaaataagc accggattcg
    44761 gcggatttct gcggcaattt ttcatcaata ataattttga gagcttcgcg agcaatggca
    44821 ctgcctaagg gatttccacc ataggttgag ccatgatcgc cgggtttgaa aacccccaga
    44881 atttcctgag aagcaactac ggctgagacg ggatacattc cgccggaaag cgctttgcca
    44941 ataattacta cgtccggtcg aacattttca tgttcgcagg caaagagttt acccgtgcgc
    45001 cccagaccgc tctgaatttc atcggcaatt aacaaaacct tattttcgcg acaaatttcg
    45061 gaagatgcct ttagccatcc ctccggtggc acgataacac cgctttcgcc ttgaatcggt
    45121 tcgaccagaa aggcaaccgt attgggattg atagcccttt tcaactcttc aatcgaacca
    45181 tattctatgg cgacaaagcc gggcgttagc ggaccaaatc cgtctttgta ttgttcatcg
    45241 gtgctgaaag aaataatcgt cgtggtgcga ccgtgaaaat tgcccgtgca aacgatgatt
    45301 tcagctttgt ctctggcaac gcctttgtat aaataacccc atttgcgggc ggctttgatc
    45361 gccgtttcaa ctgcttccgc cccggagttc atcggtaata ccatttcgta accagttagt
    45421 tcacaaagtt ctttggctag tagcggccat tggtcattac gaaaagcgcg cgaggtcagc
    45481 gtcagacggg cacattgctc ttgcataact ttgaccagac gtggatgaca atgcccctga
    45541 ttgaccgccg agtaagccgc cagaaaatcc atatactttt tgccttcgac atcctcgagc
    45601 cagattccct gaccgcgggt caaaactacg tccagcggct ggtaattatg agcaccatac
    45661 tgatcttcga tcttaatata atcctgacta cgtaacattc tttagactcc aattttttga
    45721 aatatcaaat attaagtgaa atttagccaa ttaagagtag aaaggcatcg gttttcttat
    45781 agctgactga aaacttatcg ataaataaga taaattttct gcattcgcca gcggaatgtg
    45841 atgttttttt ataaaatata cggaggttca gcgctttcgg ccaaccggca ctagataaag
    45901 cactcgttcg tctcgttcaa ccccaatgac cctggcgact gcctcgtcat taaaggctcc
    45961 caccggcaca gaagccagtc cgagcgccac tgcctgtaga tgaacgtttt gtgcggcatg
    46021 accaacttct atatggacgt agcgctcacc acgcttatga taacggcgca tcgtccgctc
    46081 ataaactgca ctaaaaacga tgatggctgg tgcagtacga acggaattct gtcccagact
    46141 ggcttcggct aaatgatcgc gacaatcacc ggcttttttt aatacgatgg catggcgttc
    46201 cggcaaatag tgaaaaattc cctccgctgt cacgagataa atttccagcg ggtaagttgc
    46261 cccggccgag ggcgccgtcc gcaatccctg tgaagagtcg ctgattccct gcgccgcata
    46321 aagcaactgg gcaatttcct tttcggacaa cggctcattc tggaatgttc gcaccgaacg
    46381 gcgttggctc aaagtcgtct caattgggac tctaccgact gcatcaggct tgggaagttg
    46441 ccttatttct ccggattcag ccgctaaatt gtttaaattg aacatcaaag cctcgcaaat
    46501 aattaatttt aaccagtaaa aatgacgttt catttctttt atcggctata cttttcctct
    46561 ttggactaat tcagtgaatt tttcaaaaga gcgatcataa gtgcgctcaa catcatcggg
    46621 gaaaaaacaa gccggtagtt gcctcgaccg cagatgataa cgaacgcaat cacaacaaac
    46681 accttttcga
//
LOCUS       DAOMJU010000005        45132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_647_length_45132_cov_18.049671, whole genome shotgun sequence.
ACCESSION   DAOMJU010000005 DAOMJU010000000
VERSION     DAOMJU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 45132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45132
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_647_length_45132_cov_18.049671"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PLO58_00940"
     CDS             <1..1136
                     /locus_tag="PLO58_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQO73386.1"
                     /translation="KSIKTVILVSNTSWYLYNFRLPLLKSLKEKGFKVITIAPKDEYT
                     SRLISNGFTHYHIPIDRRGLALFPNLITILSFISLYYRIKPKMIIHFTIKPVIFGTIT
                     ARFCTKARIVNNVTGLGYIFIGETFLHTLLQPFVQWMYRCVLRLSDLLIFQNIDDRIF
                     FIKSKIITEKIPPSILISGSGVDLERFQRKNNKNKKSEVNFILVARMLYDKGIQEYIE
                     AAKIVKKSFKNAVFHLLGDIDKGNPSAIPIEIINTWSKQGIVNYYGMVEDVVPFLENA
                     DVFVLPSYREGLARSIIEAMAMELPIITTDVPGCRETVIEKENGLLVKPNNVNDLVEA
                     MIFMINNPDIRLKMGKMSRLIAQNKFDVQQINKEYLSYLDQLW"
     gene            1195..2253
                     /locus_tag="PLO58_00945"
     CDS             1195..2253
                     /locus_tag="PLO58_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HQO73387.1"
                     /translation="MYVRILKEDENIGYNNFIAESRFSSIYQSFEWGIVKQYDHWEVI
                     RLIVIDSGQIVGGLSCLCKKSPFFAKRIFYIPRGPVLDYFAENAPDIFKLLFLKIREI
                     ARREGALYIRISPDISKNIHFNDYISKYNLHRVRYPILHTTTYRLDLTKSEEQLLNSM
                     ESRVKYDIRKAQRENVEILSDDGNEEYLKDFYNLLYQVAERNKFPIYSYEMMQNIWNI
                     LSPNGMCKIFLARSQGKIISGAFLFLFGDKCIYQWGGSVRAKNNPNQLLHWEIIRWAK
                     SNGFSIYDLQGIPENVKKGDELWGIYLFKRGFGGDRIQLIGEYDYILSPILYKIWQVI
                     EPKYPAVKNCLSKIKKRK"
     gene            2250..3065
                     /locus_tag="PLO58_00950"
     CDS             2250..3065
                     /locus_tag="PLO58_00950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQO73388.1"
                     /translation="MSKRLILAYHRILPDELALLRQPLAVSTTQFERQILYLINKGWR
                     SLTLSEYFEIYYLEQKKPDRVFVVTFDDGYRDNYYYAFPILKKYNLKATIFLVTGLLN
                     EPKTLQSDNNINNNLTEMDYSITLEQMREMANYGIEFGSHTVTHPRLDTIGLAEAEEE
                     IYQSKRMLEQYYNRQVKTFCYPYGAFNDDVISLVAKSGYQCAVVTPTRPGIKETRFTL
                     IRVGVYNSNSLLKFKFKCSNLFTNIRKARLWFLLKHLSIYLKKKSTIKTSFVW"
     gene            3310..5853
                     /locus_tag="PLO58_00955"
     CDS             3310..5853
                     /locus_tag="PLO58_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HQO73389.1"
                     /translation="MALASLLAVFLTTYICALGLTYLARKIALRYHIGDMPSERKVHK
                     VFTPYLGGAAILLSIIIGIVLSIYLLPMVAEEVPRRYYFLGITGVLIALVGLYDDIKK
                     LRFSTKFYFQILAAIIVVLGGLRFESLYIPIIGEVGLGWFSIIFTILWIVFITNAMNL
                     LDGLDGLAAGVSTIIFLAFALVALHSGRTLVALVCLLFIAANLGFLKFNSHPASIFMG
                     DTGSLFIGFSTAVISLEVGRIGHTNCLDIIIPLTILAVPILDTTISFFRRINKHMNPF
                     MADKEHIHHRLMSIGFSHKNAVKVMYFLSIIAGFIGCSFIYLDNRAIVTILFAGAIFV
                     GLVIRRLGYFEIEKNLIVVPNGTNNNSKTNGFSNGHSEPRFIPFDTSQFIQSFIFFLS
                     DVLFIVGSFLAVYYWQIVPNLLPEQLLTPYNLILWIAWSVIYWTSLLGLNNLYRIEWD
                     TSRVDEIFTVFKIIFFGTIVLFFLSFEFKIPFLPSRRILVTYGIFLVTGISVGRLVLI
                     TILKKYNLLEFKKRPTLIIGASRRALNVVRKINSVPELKFNIVGMVDDHANGNIGKII
                     EGVPIIGSFENIPEIIKKRKIKEVIIALDDNNMNQVIDMIDLFSRYNVSIKLLPDFYN
                     LLTGFRTSHIYGVSLVKFFGSNMKTWEWVLKRLIDIIISLVVLIGFLPIWLIISLIIK
                     LDSPGPVFYKQKRIGKNRREFEVIKFRSMVQGAEQETGPKWAEKNDPRVTRFGKFLRK
                     TGLDEIPQFFNVLMGDMSIVGPRPERDYFVNELERSIQFYSRRLIIKPGITGWAQIKY
                     KYDETIDDVREKLRYDLYYIENMSVILDLKIIIQTLLVGLRRKHRTSYV"
     gene            5946..7223
                     /gene="serS"
                     /locus_tag="PLO58_00960"
     CDS             5946..7223
                     /gene="serS"
                     /locus_tag="PLO58_00960"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQO73390.1"
                     /translation="MLDIKQIRNEPEKFRRGLEAKNAGDKLDELLKWDGERRKCINQA
                     DELKAIRNRVSDEIGQLKKKGLPVAEKILEMRSVGDQINNLDHKLKEIDEKINSILIT
                     LPNLPHPSVKIGKSAADNVTIKEWGHKPEFDFPLKDHLDLGEALGILDMKRAAKISGS
                     GFPMYIGDGATLERALVNYMLDFHRNHHGYQEVFPPFMVNRDSAFGTGQLPKLEEDMY
                     HIEIEDLFLIPTAEVPVTNIHRDEILPENQLPIKYTAYSACFRREAGSYGKDTRGLSR
                     VHQFNKVELVWFTTPENSYQVHEQILRDAEDILQALNLHYRVVELCSADLSFAAAKCY
                     DIEVWAPASGKYFEVSSVSNFEDFQARRANIRYRRTSDNKVNFVHTLNGSGVATPRLM
                     IALLETCQTPEGTIIIPQVLQKYTGFNQIRTKK"
     gene            7220..7876
                     /locus_tag="PLO58_00965"
     CDS             7220..7876
                     /locus_tag="PLO58_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQO73391.1"
                     /translation="MTGEKKYRKLICEIGKKIYANGYVAANDGNLSMRLNAEMVLITP
                     TNVSKGAVKPSQIAKIDLSGNPLDKNRPSSESLLHLAIYKRCDWVKAVIHSHPPYSTA
                     WAVTGRGLTDPILPETIVSIGQIPLIPYKPPSTQELADIVADAAQKNDVLLLQNHGLV
                     TVGKDLLDAYYKTERAEHVFRIVALAKQIGEIRTLSKAEVAELYRLFNVSEKLQVTYR
                     "
     gene            7884..8597
                     /locus_tag="PLO58_00970"
     CDS             7884..8597
                     /locus_tag="PLO58_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQO73392.1"
                     /translation="MIRNFFAVIWVVLVTIVFGSLSMALPGSHNTMMRKWSQTILQFL
                     GVKLQVHGLENIVNRGSKIVISNHESALDIPILMAALPIPIRFMAKKELFQIPLFGWT
                     LNLARHIPVDRKRPRKSYTVINRVAKELIRNGLSIAVSPEGTRSYDGKIAPFKKGAFR
                     LAHIHNLPILPVLILGARHCVPNKSLSIIPGIVEVHIGKEVHLSDYPTMNDCVADLRN
                     QMIVQKEQYEEKRKQGSLD"
     gene            8590..9945
                     /locus_tag="PLO58_00975"
     CDS             8590..9945
                     /locus_tag="PLO58_00975"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HQO73393.1"
                     /translation="MINCIPNRIAEPEISITKKEKSLYKAWLNQEFNLADLQTVEGYS
                     LSILSPGNRNETEGPDFHDALIMLNEKFERGDVELHLESNDWYSHGHDRDPHYNNVIL
                     HVVLNSGKLLPIKSCDNRIIPTLIMTPFAISAPDEYACLSWKHLSNDDLNQVLKTFAE
                     IRFKRKAQIWRSLILTKGAEQTLYEGLADVLGYSRNRKMFGILSQKVTLELIRKILRD
                     TDPEQRIIVLESLLFGSAGFLTKPELKVFIRDTKYSEGLYKIWVKISQKYELEELEST
                     DWHFAGSRPPNFPTLRIAALAQIINKFYPNYLAETWLRVIQNAKNFTDIRRYASEMFQ
                     QPDGLWRNHPLLKQQPGKMLIGVQRLNDLLSNLLLPFSWAIASLDNQYELIEKATTFA
                     EQVECLEVPGSVKVALNRLPLDTITLHQNYLTQGIIEFSRRYCNMNICKLCPLEKYAQ
                     K"
     gene            9932..10411
                     /gene="rfaE2"
                     /locus_tag="PLO58_00980"
     CDS             9932..10411
                     /gene="rfaE2"
                     /locus_tag="PLO58_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HQO73394.1"
                     /translation="MPKNDKIITREQAGRIVSQLKREGRKVAFTNGCFDLLHIGHLEL
                     LEHARKLGDILIVGLNSDDSVRRLKGPQRPINTQLDRARILAALEVVDYVVIFNEDTP
                     LELIQTIKPDFLIKGGDYSPDTIVGSAFVESYGGKVIVFPLVHGKSTSDLINTIFQL"
     gene            10484..12289
                     /locus_tag="PLO58_00985"
     CDS             10484..12289
                     /locus_tag="PLO58_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQO73395.1"
                     /translation="MNNRKKLRLILLFWMTITISVALSANNSYEKINNNNGEEKELSD
                     NSGNDIFELIFNEAKLFYVDALVSSYYNDTAAVKYCFDRIFEIIAEISELDSLTLVEQ
                     DDFNRFNEMVSNDYQTHFSYLIEETDSVMIVSVGEELFVTPLDTVDIGNDTLIVVEDR
                     PGHIPIVRSKKIDKLIIYYSGQQAPVIQRYLEAFNKYKDHYLPILKKYNVPEEIIYLP
                     ILESGYNPNAYSYAHAAGIWQFIAGTGAKYGLKRNWYVDERRDPIKSAHAAAKYLRKL
                     YEEFDDWYLALAAYNCGEGNVWRAIRREGTRDYFKLRSLPSQTRNYVPSFMAITIIAK
                     DPEKYGLTTPTTPTWEWEEVIIDRCYELDVIAKACETTPDILRQYNPELRRWMTPAND
                     NQYVLRVPKGKAEGLTEKLVSLPEPKDVKPEVVYHKVRKGENLSSIARKYGTSVSALV
                     SANHLRNRNQLRVGQVLLIPTGSYYSAPEKKSSQTNVVTHVVQRGETLSEIAEKYNTS
                     VSKIRSMNNIHNSHIQVGQKLKVPTNLASQIEPSDNPKGSTKVIHVVKKGDTLTSIAN
                     KYNVSLTKLKSWNDLDGRKPIYPGQRIVIYQSIKS"
     gene            12417..13313
                     /locus_tag="PLO58_00990"
     CDS             12417..13313
                     /locus_tag="PLO58_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HQO73396.1"
                     /translation="MNSINISLMSNVGHLILTMVSSEILNTERHRDGVKYFCKLFRIE
                     SSLPSINHVTSILRKFSLLPYENISKILSLNQNWDENHFRWPQEIICDYEKYKLGGTC
                     FSLTFFLKAILDYFGYQTTILMADMKAGRNTHCALLLEFKNRKYLLDPGYLLHFPLPL
                     NSAVISGKVQLEYDPFCERYSLSTLENGKKKWRYSFQERPTSIEDFSQYWEDSFHWMT
                     MHGICLSKRDENNFIYLHNHYLKLENRSQHFKGNLNTEIAELAQRWFNIPPEIVRQAE
                     KALRENLYQDKILGYQIPKWFK"
     gene            complement(13450..14316)
                     /locus_tag="PLO58_00995"
     CDS             complement(13450..14316)
                     /locus_tag="PLO58_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HQO73397.1"
                     /translation="MTNYSSSSRKSPFISNEVLRRYFDEIRTEKTLTPDEEIELARKV
                     KAGDTQALDRLLKANLRFVVSVAKKYQGYGLSLADLINEGNIGLIKAARRFDETRGFK
                     FISYAVWWIRQTILQAISENSRLVRLPLNVVGNLNKITKITSEFERDYEREPTNDEIE
                     VLLQNENIDIDSARQLTENTISIDAPVGNDQTGTLQDILPSQEDNDPGLTLNQESFHL
                     EIERALNSLDKREAYILRLYFGIDSDLPLNLEDIAERLHLTRERVRQIKEKALRKLRH
                     NSRALHLRGYLG"
     gene            complement(14344..14736)
                     /locus_tag="PLO58_01000"
     CDS             complement(14344..14736)
                     /locus_tag="PLO58_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73398.1"
                     /translation="MTGIWDDIKKSVGGFASKAAEKAGELSREAAEKAEEMTQLGKIK
                     LDIFQIKRDIDKNYTELGRVVYGLLNQNTKGNLVKNEKVTSLINNIKELEKKLKDKEA
                     QYYKIQESSKENTAKPDEPDQSKSETET"
     gene            complement(14869..16548)
                     /gene="groL"
                     /locus_tag="PLO58_01005"
     CDS             complement(14869..16548)
                     /gene="groL"
                     /locus_tag="PLO58_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561033.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQO73399.1"
                     /translation="MAKDITYEIQARTALKEGVDKLANVVKVTLGPKGRNVVIEKKFG
                     SPTITKDGVTVAKEVELEDKLENIGAQMVKEVASKTSDVAGDGTTTATVLAQSIIAEG
                     IKNVTAGADPMELKRGIDTAVNTVVESLKELSKAVTNSKEEIAQVGTISANNRKVRRM
                     IKDESGKEDIVEIPIGVLIAEAMEKVGKEGVITVEEAKSTLTTLDLVEGMQFDRGYIS
                     AYFVTNAEAMEAVLEDPYLLIYDKKISNMKELLPILEKVSQMGRSLLIIAEDVEGEAL
                     ATLIVNKLRGTLKVAAVKAPGFGDRRKEMLEDIAVLTGGKVISEEAGYKLENATISHL
                     GNAKRIVVDKDNTTIVEGAGKPEDIKGRIKQIKAQIEASTSDYDKEKLQERLAKLSGG
                     VAVLKVGAATEVEMKELKALVEDALHATRAAVEEGIVPGGGVALIRCIPALDKIKVTG
                     DQMVGVKIIKRALEEPLRQIAKNAGHEPSIVAQKIKEGKDDFGFDAYKEEYVHMYKAG
                     IIDPTKVTRIALQNAASIAGLMLTTEAVITEVPEKEPAAPAMPPGGMGDMY"
     gene            complement(16570..16854)
                     /gene="groES"
                     /locus_tag="PLO58_01010"
     CDS             complement(16570..16854)
                     /gene="groES"
                     /locus_tag="PLO58_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQO73400.1"
                     /translation="MAIKPLADRVLVKPLVSNEKTPGGIYLPDTAKEKPQEGTVIAVG
                     PGKTTDDGKKIPLEVKVGDHVLYGKYSGTEVMIDGEEHLIMRESDIFAIL"
     gene            17105..17884
                     /locus_tag="PLO58_01015"
     CDS             17105..17884
                     /locus_tag="PLO58_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQO73401.1"
                     /translation="MISSNFLFILVGFVAGVLSGLFGIGGGIVIVPIMIWFCHFSQLA
                     ANSISLTALLLPVGILAVLAYHKSKLVKVRQALFISLGIFIGVVGGAILALEIPAHFL
                     KRLYGIFLLYIAWTYIDIPSLFANGANQEKTNQQFENLNLDRPVCYIVLGFAAGIMSG
                     LFGIGGGIIIVPALISIFKYPYKKAVGTSLMALLLPVGLPGVLIYNSAGQLQIGPALF
                     IALGLLFGALVGALVAIELPTKLIKRIYGGFLLLAGFNFIF"
     gene            complement(17927..18274)
                     /gene="rplS"
                     /locus_tag="PLO58_01020"
     CDS             complement(17927..18274)
                     /gene="rplS"
                     /locus_tag="PLO58_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002833579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQO73402.1"
                     /translation="MDKLTAAVADQFRTDIPEFKPGDTVAVEVKIIEGDKERLQTFQG
                     VVIARHGSGISETFTVRKISNGIGVERIFPLHSPKISKIKRISVGKVRRAKLYYLRGL
                     RGKASKIEESNRR"
     gene            complement(18267..18977)
                     /gene="trmD"
                     /locus_tag="PLO58_01025"
     CDS             complement(18267..18977)
                     /gene="trmD"
                     /locus_tag="PLO58_01025"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQO73403.1"
                     /translation="MKIYIITAFPGMITACLSESMFRKAVENGKVEFNVWNLRDFTRD
                     KHKQIDDKPYGGGAGMVLKPEPFFRAYDKIVKLTGHKRPRVVFPTPQGRLFNHHLALE
                     LAQESDLTFFCGHYKGVDERVIANLVTDEISIGDYVLTGGELPALIIIDAVIRHIAGV
                     LHSYESAETDSFTDNLLEGPIYTHPQVYRRKSVPEVLTTGNHAKINAWRQEQRKLRTR
                     QRRIDLIKNMNMEDKNNG"
     gene            complement(18974..19480)
                     /gene="rimM"
                     /locus_tag="PLO58_01030"
     CDS             complement(18974..19480)
                     /gene="rimM"
                     /locus_tag="PLO58_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQO73404.1"
                     /translation="MDHLVLLPVGSILKSFGLNGAVLLNRGSLKNIKVDEPKVLWLGE
                     SAQVARPWKVDYLRISTKKVYLKLRDINSRQEADYLRGLKVFVPREYELKKELVELIG
                     FRVRVINSAKDIGFISSLDETNPQALFVIQSEDATLLVPAVTDLIEQIDPEQQIVIFK
                     DIEGLFPK"
     gene            complement(19489..19722)
                     /locus_tag="PLO58_01035"
     CDS             complement(19489..19722)
                     /locus_tag="PLO58_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HQO73405.1"
                     /translation="MKEFVEFIVKQLVDHPEDVKVVQVDSEKTVILELSVKEGDLGKV
                     IGKKGRTARAIRTLLTAAAAKQGQKRAILEIIE"
     assembly_gap    19885..19984
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20140..20376)
                     /gene="rpsP"
                     /locus_tag="PLO58_01040"
                     /pseudo
     CDS             complement(20140..20376)
                     /gene="rpsP"
                     /locus_tag="PLO58_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(20394..21719)
                     /gene="ffh"
                     /locus_tag="PLO58_01045"
     CDS             complement(20394..21719)
                     /gene="ffh"
                     /locus_tag="PLO58_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQO73406.1"
                     /translation="MFEQLQNGFGAIIKSLRGEGRITENNIADAMRQIRRVLLEADVN
                     YKVAKEFVQSVQEKSVGVTVAKSLSPGQLIVKIIYDEMVHLLGDKNRNLKEASIPPTI
                     IMLVGLQGSGKTTCAAKLAYQLLQKQHSPVLVPVDVYRPAAEEQLEILARQIKVPCFR
                     ENPDVVGRIQDAITFARQQHRDTLIIDTAGRLHIDEKMMAELKTIKNTIKPHNILLVV
                     DGMTGQDAVNTATSFNEQIGVDGIILTKMDSDTRGGAALTIVSSTGKPIIYMSVGEKI
                     GDLEIFHPERVAGRILGMGDVVSLVEKVQASVDLESALKLENKLKKEQFTLEDYLTQL
                     RQLKKLGPLDSVMEHLPGFARMRLGNQKVDEKELIKAEAIIQSMTRTERLKPNIINGS
                     RRRRIALGSGTQPQDVNRLLNQYWQITKLMKQMGRMKLPKNLSAFGIGN"
     gene            21920..>22937
                     /locus_tag="PLO58_01050"
     CDS             21920..>22937
                     /locus_tag="PLO58_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HQO73407.1"
                     /translation="MNRRHLMIVLTFPIFLMYNCSETQPESSNPYRINSGLNPGINIG
                     NALEAPNEGEWGVVIKDEYFPLIKSAGFNSVRLPIRWSAHADINPPYTISDDFMNRVR
                     HIVDTALEHKLYVVINVQHFQEIYTEPIENKPRLLAFWHQIGLAFKDYPQTLLFEPLN
                     EPHFNLTPELWNEWIPELIDTIRVTNPNRTLVIGTADWGGIWKMKELKLPTTERNLIL
                     AIHYYEPFRFTHQGAEWEKESNAWLGRTWTKTTDQMQELNQHFDLIKKFADSLGVPVY
                     IGEYGCYNRAPEESRLIWTRTVSEQCEKYGFSRAYWEFCSSFGIYDPISQQWREPLRS
                     AVLLA"
     assembly_gap    22938..23037
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23051..26968
                     /gene="porU"
                     /locus_tag="PLO58_01055"
     CDS             23051..26968
                     /gene="porU"
                     /locus_tag="PLO58_01055"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HQO73408.1"
                     /translation="MIINKRFCAKIIFTSFCLFSAMLVRLEAVELTILSETTSELILR
                     VEMDADLEWRQIENYPNKVSALTLGDAYQSHLSQTLEIPYYQWLIALPTEDKPSVSIT
                     KSVFESVKLLKNLTTDELTALDKFPLVAIAETGFLRDVPFGSLVIYPLRSANNSNELR
                     LLREIEITIQYTAVRKTTINQTTVEKGDYLNAFVNGSSATQWQQSRPLSLRKPQSQPA
                     GKWFRITVKKDGVYTLTLDDLKSAGLTETSIDTGRIYLYSNSTGGRPLDATIGAAVPS
                     NLIENTRWIKGNNDQDFTNADTILFYGRATSGVDLDSKNSLTFVRNPYSLINYYWLFI
                     ADEPGYPKAMPTINSLSATPNMTVTQSEHLEVHEEEKVNFLRSGLKWSGEKFNTSGSN
                     VSVILTMPKVRDSYVGVMTICTRGAVDEILHYFKAYLNNSTTPVASWSATNYQSATTS
                     FSTTLSANINILKINYTSQPSSASAYLDYVMFRYTKDLSAFTEPQTFWSLLYSGIVEY
                     QITDLNFTNLLILDVSDWSNVSIQQYNRNSNLISFRTASQSNQRRQYYLTAPSFFNKP
                     EKIELIPNPQWITLRNITSSYDYIIITADDFLTAAEKIATLYSQELSPGDRLNTLVVT
                     QNQILREFNADITDPHAIRFFLKYAYENWTPAPAYVLLLGDGTFDYRAIESSTGNYVM
                     TYQVEPAVEDYSGFSSYASDARFVYIKGSDQVMDMAIGRVPVRTATEALDYYEKLKQY
                     LTEPIYGEWRSRITLVADDPQRPYNNEKYHINDTESYIAKYIPKSLITDKLYLLEYPE
                     VQDVSSNWVKKPAATEAILNQLKRGTTLINYLGHGSSTVWAQEEVLKMDRDIGLIEAG
                     MKLPLWIAGTCSWGQFDDINTICMPEALINKNSDGAIAILTPTRPTTGTQNASFIGNF
                     IKKLFPSGEISRTRLGTLIQNIYNGSIENQEKYVLFGDPALYLALPYQKAAFDKMPRD
                     TLAALEKVSVTGRTVTEENFNGDGIINVFDSDRYVTRYYLDDAKVTQNLSYTLSGETL
                     YRGNLKIENSKFFSRFYIPKDLSYMNLFGRIKVYGWNTSAMSDFAGCYDSLLFTGTTQ
                     FDDTSGPQIVIGFEGMDFKSGDIVAPDAKIKVSIMDPVGINIAGKLGHDITFRFDNDN
                     DLIFEMTEDFVYDVNSDSSGVITSDLPDLSPGIHQITVKAWDNANNYATAIAEFVLAS
                     SDKLKLERVVNFPNPFASTTDFVFYLTQPGQVKITIYTIRGLKIKTIASDEILPPGFH
                     QVNWDGCDDFGDEIARGIYLYKVQVQSSDSNQKDTFIGKMVKS"
     gene            26965..27564
                     /locus_tag="PLO58_01060"
     CDS             26965..27564
                     /locus_tag="PLO58_01060"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQO73409.1"
                     /translation="MTTQLNWYSMPIILASQSPRRKEILDMIGLKYEVCPPHYIENNS
                     IGKSPTTIVIKHALNKARTVAPRYSKAWIIGADTVVVCKNRILGKPQNENEAVQMLKF
                     LSGVEHSVFTGYCVLNSANGRYLKNAVRTRVRFKEIPLAEIQYYVENYHPLDKAGAYA
                     IQDFSAIFVEKINGCFYNVVGFPLPAFYEQVKNELSICL"
     gene            27632..28411
                     /locus_tag="PLO58_01065"
     CDS             27632..28411
                     /locus_tag="PLO58_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HQO73410.1"
                     /translation="MATFYEILKQSREAQGLTVEQISSRTKISKEIITAIESGDLKIL
                     PKTYMRLFLKAYSQELNLDPEAILKNFEESLGEPVETPAVPDNNIKPSLPISQKPEKP
                     EKPITVKKQNRNFSTVIIVFVILVFLIAVLRQVLMDEKKTSSNTALPKITPTDTTQTI
                     PPSPDLEVAVPKSELNLTITTKDSCWVRIVIDNRTTFEATLPPLYKKEMIAKEQFDMQ
                     IGRPASINLVLNGKDLGPVGIPGIPTRLIITKDGIIKRQSF"
     gene            28582..29034
                     /gene="mraZ"
                     /locus_tag="PLO58_01070"
     CDS             28582..29034
                     /gene="mraZ"
                     /locus_tag="PLO58_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQO73411.1"
                     /translation="MITAFAGETRHPLDEKNRLSIPAKYRRWADGQDGECTFVVTKGS
                     DPCLVVYPSTEWDVLSEKLLKLSYFKKKNRAFIRALARSSVRLKCDGQGRILIPTSLL
                     EYAHIKKEVTIIGVLNRLELWDPETLEKHEESSIPLDDEYYEDLGDIL"
     gene            29059..29982
                     /gene="rsmH"
                     /locus_tag="PLO58_01075"
     CDS             29059..29982
                     /gene="rsmH"
                     /locus_tag="PLO58_01075"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQO73412.1"
                     /translation="MTTLHIPVLVEQTIQFLINRPTGIYVDCTLGTGGHFQAIAQHLD
                     NDAYLIGIDADPSAVQYCEQNISISQPHQFITANFADIKRICYRAGFPKVDGILMDLG
                     LSSFALDNPERGFTYSQAGPLDMRFSPDTAQTAMDFINTASLKELVLVFKEYGEERYS
                     QNIARRIIADRDKNPIKTTGQLASIIREEVPKQFQIKTLSRIFQAIRIQVNQELDALR
                     QGLSDAVSLLAPSGRIVVISYHSIEDRIVKQFFKKEASDCICPPDFPICQCNHHAQLK
                     ILTHRPIVPSKSEIERNSRARSARLRAAEKL"
     gene            29992..30306
                     /locus_tag="PLO58_01080"
     CDS             29992..30306
                     /locus_tag="PLO58_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HQO73413.1"
                     /translation="MSSRKSFRKKKKNLLLIIGFFVLVGSMVFYIWIYNTTNALYREI
                     DQLKRKEASLIARNQMLVVEIERLSRADRIKNIATTEIEMVTPLPETLAVVIDSKVLA
                     VE"
     gene            30306..32309
                     /locus_tag="PLO58_01085"
     CDS             30306..32309
                     /locus_tag="PLO58_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HQO73414.1"
                     /translation="MANQFKSIRKRTLIVSSSLVVLWVVLIARLFYIQVLNSKKYQEL
                     CNNQANLRKAVLPRRGIFYDRNGEALTIDLVNYSIAAHPYLVEDKPRLASTLANDLSA
                     KPEQYLKLLESNKTFVWLEREVPYSSFQNYEKYEKTPGLVIEKNVKRYYPLGEITGQL
                     LGFTNIDNRGVCGLESGLEGLLCGTPGWIMVQKDGWGRLHYRPDLPSKSSIDGNDIVL
                     TIDREYQTILYEELLQAYTETKAEKAMGIVINPNDGDILAMVTLPPFNSNKPSDYPIS
                     AQRNAVVSDIFEPGSIFKIVTATAALDNKVLQPKHQIDCNPGFIQVGNRIIRDHDKYS
                     ILTFAEVIKNSSNVGTIKVAQLTGKDQIFNYARKFGFGAKTDIQFPGEESGILHPFKK
                     WNDLVLAQVAIGQGVCVTALQTGMAYAAIANGGLLLKPQIVKTIKTKDGIEIFNAQPV
                     YIRRVASSETMSTLRELLRLTVMSGTGSRADINGLAIAGKTGTAQKVVNGAYSQTEYV
                     ASFVGFFPADNPKLLCVVVIDNPKGIRHTGGGVAAPVVREVFKRIVNQSDDLFFDEPA
                     PLPTPVVNYVYETKPAVANVPQTPDEAYHPNLTTAIYTNQMPDLCGKTLRQAVGILYE
                     LGIDDIEIDGAGVVVSQKPAPHSPINPHVKCILKLQPTRTPLE"
     gene            32302..33765
                     /locus_tag="PLO58_01090"
     CDS             32302..33765
                     /locus_tag="PLO58_01090"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQO73415.1"
                     /translation="MSNSLSEILQGIEFRRLGNLPTSVAALSVNSKELAKGALFFAIR
                     GAKDDGHKYVKNAIQNGAAAVVVEEVVADESIPQIIVADTKTALSKAAANFYENPTNN
                     MTIVGVTGTNGKTTTVYLLNEIFKEAGLKHGTVGTLGYSIGDERFSSNLTTPDSIQLQ
                     QIFKKMLNRGVEIAAMEVSSHALALHRVDDVHFKAAVFTNISQDHLDFHHSLDEYATT
                     KSKLFAMIEKDGFVINNLDDNYSDLFKNAARCRLFTYSLLKTADFTWAPGVTWSNGIS
                     GEILTPNGKIEIKSCLSGQFNLKNILAATAVAVNLNIDDKIIASALARVKSVPGRLQE
                     ISHPGQFRVFIDYAHTPDAITNVLQTLRGMLPADGRLIVVFGCGGNRDRDKRPKMARA
                     VESLADLAIVTTDNPRFEEPHDIINDIVKGFTSGYNFKVIIDRKEAILDALKNAEQND
                     IIAILGKGHETYQEIRGVRYPFYDGEIVQQFYGGSND"
     gene            33758..35107
                     /gene="murF"
                     /locus_tag="PLO58_01095"
     CDS             33758..35107
                     /gene="murF"
                     /locus_tag="PLO58_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQO73416.1"
                     /translation="MIKADFIRSLGLGRIVNFEGAINGAEIDSRKLQPGAAFFALKGA
                     TSDGHAYVQEALQKGAVLCVVAEDWAAKNPTPAPLWIVPDPEKALQKLAEKWRLSFSI
                     PVLAITGTNGKTTTRAMCAAIIKTTYKLHTTSGNFNNQLGLPITLLNLRPEHQFSLIE
                     MGTNHFGEIARLCEIAHPNAGLITNVGWGHIEFFGNIEGVAQAKSELFAALPENGTAF
                     INADDPLIVKMPVAGRKIKYGFENKDVDFYGKISGYNNEGQAVLNVNSRFDIHLSIPG
                     KVAALNALAAVAVGLFFNVDEKAVIAALEAFQPVSQRFVQIKVGPYQVINDAYNANPD
                     STIAALETFRRIKTSGRRIFIMGDMLELGQYAIQGHKRVGKAVAQADIDRFYGVGTLT
                     AYAIETAARAGLKAAYYFSTKSDLLAALKSDLKEGDTLLVKGSRGSRMEEIIEGLSQ"
     gene            35109..36212
                     /gene="mraY"
                     /locus_tag="PLO58_01100"
     CDS             35109..36212
                     /gene="mraY"
                     /locus_tag="PLO58_01100"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQO73417.1"
                     /translation="MFYHLLYPLKKYFTGFNLFQYITFRAAMAAITALLISFIIGPYI
                     IKKLKKYQIGELIREDGPQTHLAKKGTPTMGGIIILFSVIVPVLLWANLTNTYINLIL
                     LSTVWMGIFGFLDDYLKVVKKKKKGLIARYKLLGQIALGLLIGCYLYFSPEFADINSI
                     TSVPFLKNNYLDFGWFYIPFVIFFITGISNAVNLTDGLDGLAAGLMGIVALALAVIAY
                     ITGRVDFSNYLNTIYLAGSGELSIFCMAAAGAFLGFLWFNAKPAEVFMGDTGSLSMGS
                     ALATLAILLKKEILFIFLGALFIAEALSVIIQVVYFKWTKKRTGVGQRVFKMAPLHHH
                     FEMQGIPESKIVIRFWIIGILLALFSLSTFKIL"
     gene            36209..37603
                     /gene="murD"
                     /locus_tag="PLO58_01105"
     CDS             36209..37603
                     /gene="murD"
                     /locus_tag="PLO58_01105"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQO73418.1"
                     /translation="MIDLKTKPANLNDLKVAVLGAKRSGIAAAILAARRGAEVLLSDT
                     ANPEIAPEQHSALKQHNVQLEIGGHTSKIFDSDLVVLSPGIPNDAPIVIEVTKHSIPI
                     VAEVEMAYWFCPTDNIIAVTGSNGKTTTTTLLSEIFKGTQYEPYCGGNIGTAFSHLII
                     EAERSAHPQKIFILELSSFQLERIVHFRPKVSLILNITPDHLDRYEHRMELYLAAKLR
                     ITMNQTEDDYYIFNDDDELLKTNLPPRCHLIPAGLHSTSQKLITSDAEKIYFSGQDQF
                     IERARVALLGEHNLYNLTAALTAGRLYQLPITHLQNVLYNFKSIEHRLEFVTTIDGVD
                     YYNDSKATNVDSVNYALKSFNRPVIVILGGKDKDSDFTLLIPQIREHVKAAVLIGKAA
                     DKIRKALSGVVEMYDAGYSMPNAIEIARNLAVPGDIVLLSPACASFDMFNDYEHRGRV
                     FKEIVMNLPKDLKV"
     gene            37600..38742
                     /gene="ftsW"
                     /locus_tag="PLO58_01110"
     CDS             37600..38742
                     /gene="ftsW"
                     /locus_tag="PLO58_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HQO73419.1"
                     /translation="MNVAFKKGKVDQKYLIAFFILLGFGLVMQYSASSSLGEARFDDP
                     SFYVRGQFIRIVLGMVVGLICTFINYQILKKGAFWIALAGIIALIATLVYHKLHPGQA
                     TARWFPIGGFNFQPSEFAKLALIIYLSSFIERHERHLDDFKRGFIPPVIIMLIMVILI
                     IVEPNYSTAAVFTVFGLIVLFIGGTKLLHFLPFIGGFALLSVVTILRSPYKLMRILTF
                     LEPEKDLQGAGYQIHQSMITLGNGGFFGRGLGGSIEKNLFLPDMHTDFIFSVVGEELG
                     FIGAVVLLSLYLYLFIRGIKIALRAPDVFGNLLGIGLSSCLFIYVIVNVGVTCGLLPV
                     TGLPLPFISYGGSSMLFNFACVGIILNISRYAVPQEKTNSLVMFND"
     gene            38735..39874
                     /gene="murG"
                     /locus_tag="PLO58_01115"
     CDS             38735..39874
                     /gene="murG"
                     /locus_tag="PLO58_01115"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQO73420.1"
                     /translation="MTKKDESRRLKVLITGGGTGGHLFPAIALAEELQMRQSQIGELE
                     IIFIGTRRGLESQIVPSLGFEFHKIWIRGWQRGWTLNDILVNLLFPVRLFVSLIQSFF
                     IIKNFDPDLAIGTGGYTAGPPLRIAAMLKIPIFLHEQNVIPGATTKMLANKAKRLYTS
                     FEESKTYLENAICLGTPLRRSLQIVPREQAIYFFELEPDRKTVFIFGGSQGSQAINSY
                     WIENIPTVVEAVKCQFIWQTGQRDFKMIDNIFSNNPWVHVTPFIHEMGIAYCAADVVI
                     SRAGALSLAELCLYGKPSILVPLPTAAGNHQEINARNMEQRGASILVLQKELTNDRLT
                     QELIALLKDEPRLQAMSANAKALARPDSAKLIIDDILGFIEVNAL"
     gene            39864..41222
                     /gene="murC"
                     /locus_tag="PLO58_01120"
     CDS             39864..41222
                     /gene="murC"
                     /locus_tag="PLO58_01120"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQO73421.1"
                     /translation="MHSEVKKYHFVGIGGIGMCGLAEYLLHNGQIVTGSDLAPSENTE
                     RLENLGAQIKYGHRRENVGDCDYLVYSSAVKPDNPERMVAIERQIPTLRRAELLGQIF
                     ATKPIRIAVSGTHGKTTTTAMIGQVLTTAGMDPLILSGGVLKSSGSPVQIGKGDIVIA
                     EADEFDRSFLQLSPTHAIITNIESEHLDCYANLTQIKNAFYEFTAKIPDAGRVIACSD
                     EKEIPDFLKHHPRPYVTYGLYSGEFQARNIQLKGNSSNCEVYHNSDLIGSLQLHVPGL
                     HNIKNGLAAIAMASLLNIPFPVIQEGLADFRGVKRRFEILQENDNLMLVDDYAHHPSE
                     IAATLNAARKGWQRRIIAVFQPHLFSRTRDFYPEFARVLRVADVIVLLEIYPAREAPL
                     EGISSRLIYDEIRKDRTQDVYLTNTLAEALALLQEIKLTGDMIITLGAGDVNKILPKL
                     ME"
     gene            41544..42344
                     /locus_tag="PLO58_01125"
     CDS             41544..42344
                     /locus_tag="PLO58_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HQO73422.1"
                     /translation="MTAEKATKISRNDQAKRPRKKVWFSLCLALIGGFGYLVYLAIDW
                     CEYTNFFNLDKVEVSGVSILSNEEIIKIAGLKNHVSLLKLDIHQIQNRLEMEPYIEAA
                     VVSREFPNHLKIMIKERIPVCYLNTGTLWLIDQQGIILPLRKEKLKANLPVITGFEKD
                     SARCRPGQLAPNPEMFKALNFIQTAALKANTLYTEISEIFAWKKGEFIIYTVNGGTPI
                     YIGRGDLTKQLYILAAFQNLIKSKRTFADYQYLDLRWDKQIIARERKS"
     gene            42345..43616
                     /gene="ftsA"
                     /locus_tag="PLO58_01130"
     CDS             42345..43616
                     /gene="ftsA"
                     /locus_tag="PLO58_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQO73423.1"
                     /translation="MKMLKLKNQILTGLDVGSSKVCAVIAEVTPDSPEPIIKGYGISP
                     SKGIKKGLVVDIENATQSIVNAVSEAEKMAGFSINSCLVGVSGDHIRSLNTRGMMAIS
                     RDSRTGLGEAREIEIEDIERVIEHTKAVPLPLDRQILHVLPQEYIIDEQPGVKNPLNL
                     SGRRLDAKVHLTTYNTTVLSNLSKCIKNADLEIDGFVLSSLAAAYACLEESEKTMGSV
                     LLDIGSGVIDIIVFYDDGVHHTGVVNLGANSVTNDIAYMLRVPLEKAESIKREYGFAK
                     VSLTDREAFFRLDSLGGRSPREISIFNLAEWIEPRMEEILRESYLEAKKADIPLTNTL
                     SVVLTGGGARLKGTDELAEMIFNTPARVAAPRGFQEYVTELSDPIYSVAIGLIKYAIA
                     GQISGSALNKTISGPFHRIRGWIKHVTENVM"
     gene            43632..44831
                     /gene="ftsZ"
                     /locus_tag="PLO58_01135"
     CDS             43632..44831
                     /gene="ftsZ"
                     /locus_tag="PLO58_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQO73424.1"
                     /translation="MLFEFEELVNQQARLKVVGIGGGGGNAITRMIENGLIGVDFIAL
                     NTDVQALECCKAPTKFQIGRALTRGLGAGAIDDIGRKAAEADRQTIASMIEDTDMVFI
                     TAGMGGGTGTGAAPIVAQIAREQGSLTVGIVTKPFNFEGAKRMARAEEGINQLRKCVD
                     TLIVIPNQKLLAIVDHDTSILDVFKTADSVLYQAIRGISDLINKNGVINLDFADVRTI
                     MKDMGKAIMGTGLATGEERAVLAAQQAISSPLLDEVNIKDAKGLLINITGPANLDIHE
                     VDEACSIITDEVGANAETIFGMVVDENYTDEIMITVIATGFGEAPTPINLSQNKKDFS
                     SPIKDEPRNTDDIWNRINEPGQPVSLFSDDQVIKILNPNDMGIADNPSHKIHQTNIPT
                     YIRQKIK"
     gene            complement(44917..45111)
                     /gene="rpmB"
                     /locus_tag="PLO58_01140"
     CDS             complement(44917..45111)
                     /gene="rpmB"
                     /locus_tag="PLO58_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQO73425.1"
                     /translation="MSKVCDICGKKPIHGNKVSHANNRTHRIWEPNLRKIRIVDNSGS
                     IKTVKVCLTCLKSGTIQKAV"
BASE COUNT        13160 a         9239 c         9032 g        13501 t
ORIGIN      
        1 ataagagtat aaagacagtc attttagttt caaataccag ttggtattta tataactttc
       61 gtttaccact tttaaaaagt ttaaaggaaa aaggatttaa agtaattaca attgcaccaa
      121 aagatgaata tacaagccgt ttaatatcaa atggatttac tcactatcat attccaattg
      181 atcgccgcgg attagctcta tttccgaatt taattacaat actttcattc atatcacttt
      241 attaccgtat aaagcccaaa atgattatcc atttcacgat taaaccagtg atatttggaa
      301 caatcaccgc ccggttttgc actaaagcaa gaatagtaaa taacgtaact ggactagggt
      361 atatttttat tggagagact tttttacata cattattgca gccatttgtg caatggatgt
      421 accgctgtgt tcttagacta tcagatttat taatttttca aaacatagat gaccgtattt
      481 tttttatcaa gtctaaaata attacggaaa agataccgcc aagtatttta atcagtggtt
      541 ctggagttga tctggagaga tttcaaagaa aaaataataa gaataaaaag tcagaagtaa
      601 attttattct agtagccagg atgttatatg ataagggtat tcaggaatat attgaagccg
      661 ccaaaatcgt aaaaaagtca tttaaaaatg cagtgtttca cctccttggt gatattgata
      721 aaggcaatcc atcggcgatt ccaattgaaa taattaacac ctggagtaaa cagggaattg
      781 taaattatta tggaatggtt gaagatgtag taccatttct ggaaaatgcc gatgtctttg
      841 ttttgccttc atatagagaa ggattagcac gctcaattat tgaagctatg gctatggaac
      901 tcccaattat taccaccgat gttcccggtt gtcgagaaac ggttatcgaa aaagaaaatg
      961 gattgctagt caaaccaaat aatgttaatg atttagtaga ggctatgatt tttatgataa
     1021 acaatcccga tatacggtta aaaatgggaa aaatgtctag actgattgct caaaataaat
     1081 ttgatgtcca acaaattaat aaagagtatt tatcttatct ggatcaacta tggtagtaaa
     1141 ttattacata aatattatca ctttttaaag cgatataaaa gggatggttt tggtatgtat
     1201 gttcggatac tcaaggaaga tgagaatatc ggatacaata attttatagc cgaatcccgt
     1261 ttctcaagta tatatcaatc ttttgaatgg ggaattgtca aacaatacga ccattgggag
     1321 gttattcgac taattgttat tgattcaggc cagatagttg ggggactgtc ttgtttatgc
     1381 aagaaatcgc cattctttgc taaacgaatc ttttatattc ctcgtgggcc ggtattggat
     1441 tattttgcgg aaaacgcacc tgatattttc aaattacttt ttttgaaaat acgggaaatt
     1501 gcacgccgtg agggggcttt gtatatcaga ataagtcccg atattagtaa aaatattcat
     1561 tttaacgatt atatttcaaa atataattta caccgcgtcc gttacccgat tttacatacc
     1621 acgacttatc gcctcgatct tacaaaatct gaagaacaat tgctgaactc gatggaaagt
     1681 cgggtaaaat atgacataag gaaagctcag cgtgaaaatg tagaaatttt atctgatgac
     1741 ggtaatgaag aatatttaaa agacttctat aatttgttat atcaggtagc cgaacgaaat
     1801 aaatttccga tttactcgta tgaaatgatg caaaatatct ggaatatttt gtctcctaat
     1861 ggaatgtgta aaatttttct agctagaagc caaggcaaaa taatctccgg cgctttttta
     1921 tttcttttcg gtgataaatg tatttatcaa tggggtggtt cagtaagggc caaaaataat
     1981 cccaatcaat tattacattg ggaaataatt cggtgggcga aatcaaacgg atttagcata
     2041 tatgatcttc agggtattcc agaaaatgtc aaaaaaggcg atgaattatg gggcatatat
     2101 ttattcaagc gcggatttgg tggcgatcgg attcagttaa ttggagaata tgattatatt
     2161 ttatctccga ttttatacaa gatttggcaa gtaattgaac caaaatatcc agccgtgaaa
     2221 aattgtttat caaaaataaa gaagaggaaa tgagtaagag attaatttta gcctatcatc
     2281 ggattcttcc tgatgaatta gcactcctaa gacagccttt ggcagtaagt accacacaat
     2341 ttgagagaca aattctctat ttaataaata aaggatggcg aagtctaacc ttatcagaat
     2401 attttgaaat atattattta gaacaaaaga aacctgatag agtttttgta gtcacttttg
     2461 atgatgggta ccgtgacaat tattattatg catttccaat tttaaaaaaa tacaatctta
     2521 aagcgactat ttttttagtg actggactat taaacgagcc taaaactttg caatctgata
     2581 ataatattaa taacaattta accgagatgg attattcgat tacactagaa caaatgagag
     2641 aaatggctaa ttatggaatt gaatttggtt cgcatactgt gacccacccg agattggata
     2701 cgatcgggtt ggcggaggct gaagaggaaa tttatcaatc aaagaggatg ttggagcaat
     2761 attataaccg acaggtgaaa actttttgtt atccttatgg cgcatttaac gacgatgtta
     2821 tttcattagt tgccaaaagt ggttaccaat gtgccgtcgt tactccgact cgtccaggga
     2881 ttaaagaaac tcgatttacc ttgattcggg ttggtgtcta taattctaac tcactcttga
     2941 aattcaaatt caaatgttca aacctattta caaacattag aaaagcaaga ttatggtttc
     3001 tgttgaagca tttatcaatt tatttaaaaa agaaaagcac aattaaaacc tcctttgttt
     3061 ggtgaaatat aataaccggg ttggagaata aaaattattt gctgataata caaaaattac
     3121 ttgacctcat caaaaaatac ttgtagattt aatcacgatg agtaaagaag aggaaaaaga
     3181 ctaaatcaac ttggtaccgg cctcgattct ttaaataacc ctaaaattta tcaacttaga
     3241 aaaactgtaa atcttatgtt ttgagtagaa atttaatatt catttagtaa tcttggctgg
     3301 aggaatgtag tggctttggc atctttatta gcagtctttt tgactaccta tatatgcgcg
     3361 ttaggattga cttatctagc acgcaagata gctctccgtt atcatattgg cgatatgccc
     3421 agtgaacgga aagttcataa agtgtttacg ccttatttag gtggtgcggc aattctcctg
     3481 tcaatcataa ttggtatcgt attatcaatc tatttattac ctatggtcgc tgaagaggtc
     3541 ccgcggcgat attatttcct aggtataacc ggtgtgctaa tcgccctggt aggattgtac
     3601 gacgatatta aaaagttacg tttttctacc aaattttatt tccaaattct cgcggctatc
     3661 atcgtggttt taggtggctt aagatttgaa tctttatata ttccgattat tggtgaggtt
     3721 gggttaggtt ggttttcgat aatctttacg atattatgga ttgtatttat aaccaatgcg
     3781 atgaatttgc tggatggatt ggatggacta gcggctggag tttctacgat tatttttctg
     3841 gcttttgcat tagtagcatt acattccggt cgaacattgg tagccttagt ttgtctatta
     3901 tttattgcag ctaacttagg atttttaaaa ttcaattctc atccggcttc aatctttatg
     3961 ggtgatactg gttcgctttt tattggtttt tcaacggcag ttatttcatt agaagtaggc
     4021 cgaattggtc acactaattg tctggatatt attattccgc ttacgatttt agctgtaccg
     4081 atattggata ctacaatttc ctttttccga cgaatcaata agcatatgaa cccatttatg
     4141 gcggataagg aacatattca tcatcgttta atgtcaattg gattttcgca taaaaatgcg
     4201 gtcaaggtaa tgtatttttt aagtattatt gccggattta tcggctgttc tttcatttac
     4261 cttgataatc gggcgatcgt gacaatttta ttcgccggtg caatttttgt aggtttagtg
     4321 atacgccgac tgggttattt tgagatcgag aaaaacctaa tcgttgtgcc caatggtacc
     4381 aataataaca gcaagaccaa tggatttagc aatggacatt cagagccaag atttatcccg
     4441 tttgatacgt cgcagtttat tcaatccttt atctttttct tgagtgatgt cttatttatc
     4501 gttggttcgt ttttagcggt ttattattgg cagattgttc ccaatctttt accggagcaa
     4561 ttgttgactc cctataattt aattttatgg atcgcctggt cagttatcta ttggacttcg
     4621 ttattgggct tgaataatct ttaccgaatt gaatgggata cttcgcgagt tgatgaaata
     4681 tttactgttt ttaaaataat atttttcggc acgattgtat tattctttct gagttttgaa
     4741 ttcaaaattc cctttcttcc ttcgaggcga atattagtca cttatggtat ttttctggtt
     4801 actggtatta gtgttggtcg tttagtatta attactattt tgaaaaaata caatttgtta
     4861 gaatttaaaa aacgtccgac attaattatc ggtgcttcac gtcgtgcgct caatgtagtt
     4921 cggaaaataa attctgtccc tgagttaaaa tttaacatag ttggaatggt tgatgatcac
     4981 gccaatggaa atatcgggaa aataattgaa ggggtaccga ttattggtag ttttgaaaac
     5041 attcccgaga ttattaaaaa aagaaaaatt aaagaagtca ttattgccct tgatgacaat
     5101 aatatgaatc aggttattga tatgattgat ctgttttcaa ggtacaacgt ttcgatcaaa
     5161 ttactgcctg atttttacaa tctactcacc ggttttcgaa ccagtcacat ttatggagtc
     5221 tcattggtaa agtttttcgg ctctaatatg aaaacctggg aatgggtatt gaaacggctc
     5281 attgatatca ttatttcgtt agtagtttta atcggatttc tacctatctg gctaatcata
     5341 agtttgataa ttaaactcga ctctccggga ccggtatttt ataaacaaaa aagaattggc
     5401 aaaaatcgtc gcgaatttga agtaattaaa ttccgttcga tggttcaggg tgccgagcag
     5461 gaaaccggac cgaaatgggc tgaaaaaaat gatccgcgag tcacccgttt cggtaaattt
     5521 ttgcgaaaaa ctgggctcga tgagattccg caatttttca atgtcctaat gggtgatatg
     5581 agcattgtgg gaccgcgtcc cgagcgggat tattttgtta atgagttaga acgttccatt
     5641 caattttact cgcgtcgctt aatcatcaaa cccggaatca ccggctgggc gcagattaaa
     5701 tataaatacg atgaaaccat tgatgatgta cgcgaaaaat tgcgctatga tttatattac
     5761 atcgaaaata tgtcagttat tttagactta aaaattatca ttcagactct tttagttggt
     5821 ctccgtcgta aacaccgcac cagctacgtc tgaaaattag cgactggaaa taacgattag
     5881 attataatat actctattat cattccaaaa ttttttctat tttataaggt tgaaatggag
     5941 aatgaatgtt agacattaaa caaattagaa acgaaccgga aaaatttcgg cgaggattgg
     6001 aagccaaaaa tgccggcgat aaattggatg agctgttaaa atgggatggc gaacgccgaa
     6061 aatgcattaa ccaagctgat gaattgaagg ctatccgcaa tcgggtcagt gacgagattg
     6121 gccaattgaa aaaaaaaggg ttaccagtcg cagaaaagat cctggaaatg cgctcggtag
     6181 gcgatcaaat caataacctc gaccataaat tgaaagaaat tgatgaaaag ataaatagta
     6241 tcctaattac tttacctaat ctgccgcacc cgagcgttaa aatcggaaaa tcagccgctg
     6301 ataatgttac tataaaagaa tgggggcaca aaccggagtt tgattttccc ttaaaagatc
     6361 atttagattt aggagaagca ctgggaattc ttgatatgaa aagagctgcc aaaatttcag
     6421 gctcgggatt tcctatgtat atcggagatg gtgctactct cgagcgcgcc ttagtcaatt
     6481 acatgctgga ttttcatcgg aatcatcacg gatatcagga agtctttccg ccatttatgg
     6541 ttaaccgtga ttcagccttc ggtaccggtc agttacctaa actcgaagaa gatatgtatc
     6601 acattgagat tgaggacctt tttctaatcc cgacagcgga ggtaccggta acgaatattc
     6661 atcgtgatga aatacttcca gaaaaccaac tgccgattaa atatacagct tactctgcct
     6721 gttttcgacg ggaagccggt tcgtatggca aagatacccg cggtttatca cgcgtgcatc
     6781 aatttaataa agtggagttg gtttggttca ccacacctga gaactcatac caagtgcacg
     6841 aacagatact cagggacgcc gaagatattt tacaggcttt gaatctccat tatagagtgg
     6901 tggaactttg ttcggctgat ttgagttttg cggcggctaa atgttacgac atcgaagttt
     6961 gggcaccggc aagcggaaaa tatttcgaag tctcaagtgt cagtaatttt gaagattttc
     7021 aggctcgccg ggcaaatatc cgttaccgcc gaaccagcga taataaggta aatttcgtgc
     7081 atactctaaa tggttccggt gtagccactc cccgacttat gattgcatta ctcgaaacct
     7141 gccaaacgcc ggaaggcaca atcataattc cgcaggtttt acagaaatat actggattta
     7201 atcagataag gaccaaaaaa tgacaggtga aaaaaaatat agaaaactta tttgcgaaat
     7261 cggcaagaaa atctatgcca atggttatgt tgcggctaat gacggcaatc tcagcatgcg
     7321 tttgaatgcg gaaatggttt taattacacc gactaatgta tccaaaggtg cggtgaaacc
     7381 ttctcaaata gctaaaatag atttatctgg aaatccatta gataaaaatc gtccgtcgtc
     7441 ggagtcactc ctgcatttgg cgatttataa acgatgtgat tgggtcaagg cagtcattca
     7501 ttcgcatccg ccatattcga ctgcctgggc agtaaccgga cgaggattga cggacccaat
     7561 cttgcctgaa actatcgtat caatcggaca aatcccgtta atcccgtata aacctccttc
     7621 aactcaagaa ttggcagata tagtagccga tgcggctcag aaaaacgatg tgctattact
     7681 ccaaaatcat ggattagtaa cagtcggcaa ggatttattg gatgcctatt acaagaccga
     7741 acgcgccgag catgtctttc gaattgtggc gctggctaaa caaataggag aaattagaac
     7801 cctgagcaaa gcagaggttg cagaattgta tcgtttattt aatgtatccg aaaaactaca
     7861 agtaacttat cgatagttct tatatgatta ggaatttctt tgctgtaatc tgggtagtgt
     7921 tagttacgat tgtatttggt agcctgagca tggctttgcc aggaagccat aatacaatga
     7981 tgcgcaaatg gtcgcagacg attctccaat ttcttggagt caaattacag gttcacggcc
     8041 ttgaaaatat agtaaatcga ggttcaaaaa tcgtaattag caaccatgaa agtgctctgg
     8101 atattccgat cttgatggcg gctttaccga tccctattcg ttttatggca aaaaaggaat
     8161 tatttcagat tcctcttttt ggatggacac ttaatctggc gcgtcatata ccagttgacc
     8221 gtaaaagacc tcgcaaatca tatacggtaa ttaaccgtgt tgctaaagaa ttaatccgga
     8281 atgggctcag tatcgctgtc agtccagagg gcactcgcag ttacgatggt aaaattgcac
     8341 cttttaaaaa gggcgctttc cgattagctc atatacataa cctcccgatt cttccggtat
     8401 tgattctcgg agctcggcac tgcgttccga ataagagcct ttcgatcatt cccggaatag
     8461 tcgaagtgca tattgggaaa gaagtccatt tgagtgatta tccgacaatg aatgattgcg
     8521 tggctgattt acgtaaccaa atgattgtcc aaaaggaaca atacgaagaa aaaaggaagc
     8581 aaggtagcct tgattaactg catcccaaac cggattgccg aaccagaaat atccattacc
     8641 aaaaaggaaa aatcccttta caaagcctgg ttgaatcagg aattcaatct cgcggatttg
     8701 caaacggttg aaggctactc gctcagtata ttatcacccg ggaatcgaaa cgaaactgaa
     8761 ggtccggatt ttcacgatgc tttgattatg ttaaatgaaa agttcgagcg aggtgacgtt
     8821 gaattacatc tcgaatcgaa tgactggtac agccatggac acgaccggga ccctcattac
     8881 aataatgtga ttttacacgt ggttttaaac tcaggtaaat tactaccgat aaaatcttgt
     8941 gacaaccgta ttattcccac attgatcatg acgccatttg cgatctcagc acctgatgaa
     9001 tatgcttgct taagctggaa acatctatct aacgacgatc tcaaccaagt tctaaaaaca
     9061 ttcgccgaaa ttcgcttcaa acgtaaagcc caaatatggc gctcactgat tttaacaaaa
     9121 ggcgctgagc agacattata tgaaggactg gctgatgtgc tgggatacag tcggaatcgg
     9181 aaaatgtttg gaatactaag ccaaaaagtg acccttgaat tgatacggaa aattttacga
     9241 gatactgatc ccgaacagcg cattatagta ttggaaagtc ttctatttgg ttcggctgga
     9301 tttctgacca aacctgaatt aaaagtattc atcagagata caaaatattc agaaggttta
     9361 tataaaatct gggtgaaaat atcgcaaaaa tacgaactgg aagaattgga atcaacggac
     9421 tggcatttcg ccggatcgcg accgccgaat tttccgactt tgcggattgc ggcactggcc
     9481 cagataatta ataaatttta tcctaactat ctagctgaaa cttggttaag agtaattcaa
     9541 aatgccaaaa attttactga tattcgacgc tatgcaagtg aaatgtttca acaaccggac
     9601 ggactctggc gcaatcatcc attattgaaa caacagccgg gaaaaatgct gatcggcgtt
     9661 cagcgcttga atgatttgtt gagtaatctg ctattgccat tcagctgggc gatagcgtca
     9721 ctcgacaatc agtatgaatt aattgaaaaa gccactactt tcgcggagca ggttgagtgc
     9781 cttgaagtgc cgggatcagt taaggttgct ctgaaccgat tgccactgga tactatcact
     9841 ttgcaccaaa attatttaac tcagggaata attgagttct cacgtcggta ttgtaacatg
     9901 aatatttgta aattatgtcc gttagaaaaa tatgcccaaa aatgataaaa taatcacgcg
     9961 tgaacaagcc ggccgaatcg ttagtcaact caaaagagaa ggccgaaagg ttgccttcac
    10021 caacggctgc tttgatcttc ttcacattgg ccatcttgaa ctattggagc atgctaggaa
    10081 attaggtgat attctgatag tcggtttaaa ttcagatgat tcggtacgac gcttaaaagg
    10141 tcctcagcgc ccaattaaca ctcaattaga tcgtgctcgg attcttgccg ctctggaagt
    10201 ggtagattat gtagtaatat ttaatgaaga taccccttta gagttaattc aaaccattaa
    10261 gcctgatttt ctgatcaaag gtggtgatta tagcccggat actattgttg gtagtgcatt
    10321 cgtcgaatcc tatggcggaa aggtaattgt atttcccctg gttcatggca aatcaacttc
    10381 cgacctgata aataccatat ttcaacttta atcaataaat gctcttgaca ttaagttttg
    10441 tttgaaataa tttcccaccc aaaaatatat agaatggagt gaaatgaata atagaaagaa
    10501 attgagatta atcttattat tttggatgac gataacaatt agtgtagcat tatctgctaa
    10561 taattcctat gaaaaaataa ataacaacaa tggagaagag aaagaactat cagataattc
    10621 tgggaatgat atttttgaac ttattttcaa tgaagctaag ttattctatg tagatgcttt
    10681 agtatcttct tattataatg acacagccgc cgtaaaatac tgttttgatc gcatatttga
    10741 aattatcgcc gaaatcagtg aacttgatag cctgactctg gttgaacaag atgacttcaa
    10801 ccgttttaat gagatggtat caaatgacta tcaaacccat ttttcgtact tgatagaaga
    10861 aactgactca gtgatgatag tttcggtagg agaggaatta tttgtaacac cattagatac
    10921 ggtcgatata ggcaatgata ccttaatcgt ggtcgaagac cgaccaggac atattccaat
    10981 cgttcgatcc aaaaaaatcg ataaacttat aatatattat tccggtcagc aagcccccgt
    11041 aattcaaaga tatcttgaag cattcaataa atataaagat cattatctcc cgattctaaa
    11101 aaaatataac gtaccggaag aaattattta tttaccaatt ttagaaagcg gctataatcc
    11161 taatgcctat tcttatgccc atgccgccgg aatatggcaa tttatcgctg gtactggcgc
    11221 caaatatggt ctaaagcgta actggtatgt agacgagcgt cgtgatccga ttaagtctgc
    11281 ccatgcggca gctaaatatc ttagaaagct ttatgaagaa tttgacgact ggtatttagc
    11341 tttagcggct tataattgcg gtgaaggtaa tgtctggcgt gctatcagac gcgaaggcac
    11401 ccgtgactac ttcaaattac gctcgttacc ctcccaaact cggaattacg tcccatcctt
    11461 tatggctatc acaatcattg ccaaagatcc ggaaaagtat ggacttacca cacccaccac
    11521 acccacttgg gaatgggaag aagtgataat agatcgttgc tatgaactcg atgtcattgc
    11581 caaagcttgt gaaactacac ccgacatcct gcggcaatat aatcccgaac tgcgacgctg
    11641 gatgacccca gctaatgata atcaatatgt actgcgggta cccaaaggta aagccgaggg
    11701 tttaacagaa aaattggtat cactaccaga accaaaggac gtaaaacccg aggtggttta
    11761 tcataaagtt cggaaaggtg aaaacttgag ttcaattgct cgtaaatatg gcacctcggt
    11821 ttcggcttta gtttctgcca atcaccttcg taatcgcaac caattacgtg tgggtcaagt
    11881 gctcctgata ccgactggct cctattattc ggcacctgaa aaaaaatcgt cccaaactaa
    11941 tgtagtaacc catgtcgtcc aacgaggtga aacattaagt gaaattgctg aaaagtataa
    12001 taccagtgta agtaaaataa gatcaatgaa taatattcat aatagccata tacaggttgg
    12061 tcaaaaactc aaggtaccta ctaatctagc cagccagata gaaccttccg acaatcccaa
    12121 gggcagtact aaagtaatcc acgtcgttaa aaaaggagat actttaactt caatcgccaa
    12181 taaatacaat gttagtctta caaaattgaa atcgtggaac gatctggatg gtcgcaaacc
    12241 aatttatccc ggtcagcgga tcgtaattta ccagtcaatt aaaagctaag tcaaacggtt
    12301 ttccctttct gaaagtctga aatgttgtgt catataaatc gtacttggtc aaaatcctct
    12361 actattatga caaaaggata agcctaatta tagaaaaata atggcttgcc tttattatga
    12421 attctataaa catctctttg atgtctaatg tcggtcattt aattttgact atggtatctt
    12481 ctgaaatcct taataccgaa agacaccgcg acggggtaaa atatttctgc aagttattcc
    12541 gtatcgaatc ttcactacct tccataaacc acgtcaccag tattcttcgc aaattttcac
    12601 ttcttcccta tgaaaatatc agcaaaatcc ttagtctgaa ccaaaattgg gacgaaaatc
    12661 atttccgctg gcctcaggaa atcatctgcg actatgaaaa atataaatta ggcggtacct
    12721 gtttctctct tacttttttt ctaaaggcaa ttctcgatta ttttgggtat caaaccacaa
    12781 tattaatggc cgatatgaaa gccggacgaa atactcactg cgcactcttg cttgaattca
    12841 aaaaccgtaa atatctactt gatcccggct atttactcca cttcccttta ccattaaact
    12901 cggccgtcat ttctgggaaa gtccaattgg aatatgatcc attttgtgag aggtattcac
    12961 tctcgacatt agaaaatggc aagaaaaaat ggcggtattc atttcaggaa agaccgactt
    13021 ctatcgagga ttttagtcag tactgggaag attcctttca ttggatgact atgcacggaa
    13081 tctgcctgtc caagcgcgac gaaaataatt tcatttacct gcataatcat tatttaaaat
    13141 tggaaaacag atcccagcat tttaaaggaa atttgaatac ggaaattgca gaactggctc
    13201 agcgttggtt taatattcca ccggaaattg ttcggcaagc cgaaaaagct ttgcgtgaaa
    13261 atttatacca agataaaata ttaggttatc aaataccaaa atggtttaaa tagcaattgc
    13321 taaaacactt gaaaaaataa aatatattaa aaatcttgct taataaccac ttagaaaaca
    13381 gattgtggtt aaaatatcag gctagactaa ctcttaattt tttagtttga gaaaaatact
    13441 ggggtatttt tatcccaaat aacctcttaa atgtaaagcc ctactattat gccgtaattt
    13501 tcgtaaggct ttttctttaa tctggcgaac ccgttctcgc gttaaatgta aacgttcggc
    13561 aatatcttcc agatttaacg gtagatcgct gtcaatacca aaataaagac gcaggatgta
    13621 ggcttccctt ttgtctaaag aatttaatgc tcgctcgatc tctaagtgaa atgactcctg
    13681 gtttagagta agtccaggat cattatcttc ctggctaggt agaatgtctt gtaacgtacc
    13741 ggtttgatcg ttgccgactg gagcatcaat actgatagta ttttcagtta attggcgggc
    13801 agaatctatg tcaatatttt cattttgcaa caacacttca atttcatcgt tggtcggctc
    13861 tcgttcataa tcccgttcaa attctgaagt aattttggta atcttgttaa gattaccgac
    13921 tacgttaagc ggtaagcgaa ccaaacgtga attttctgag atagcctgca agatagtttg
    13981 ccttatccac cataccgcgt aggagatgaa tttaaatcca cgagtttcat caaaacggcg
    14041 cgccgctttg attaaaccga tattaccttc attaatcaaa tcagcgagag aaagaccata
    14101 accctggtat tttttagcaa ctgatactac aaaccgaaga ttagctttta gaagccgatc
    14161 aagagcctgg gtatcaccgg ctttgacttt tctggctagt tcaatttcct catccggtgt
    14221 gagtgtcttt tcagtccgga tttcatcaaa ataacggcgt aaaacctcgt tggagatgaa
    14281 tggtgatttt ctagatgaag atgaataatt agtcatggcc atttccggcc aggttaataa
    14341 tggttaagtt tctgtctcac ttttagattg atccggctca tccggtttag cagtattttc
    14401 tttagaactt tcctgaatct tgtaatactg ggcttcttta tcttttaact ttttctcgag
    14461 ttctttaata ttattaatca aactggttac cttttcattt ttaacgagat tgcctttagt
    14521 attttgattc agcaatccat aaactactcg acctaattcg gtgtaattct tatcaatatc
    14581 cctcttgatt tgaaaaatat ccagtttaat tttgcccagc tgggtcattt cctcggcttt
    14641 ctcggcggct tctcgagata attcaccggc tttttcagcg gctttagatg caaatcctcc
    14701 gacacttttt ttaatatcat cccaaatacc agtcatattg tcctcctaat tttttttctt
    14761 actatttaaa aaatccacct aaaattattt ataataaaac ataatgtcca caaaaaataa
    14821 gagcctccta caattaggag gctcttttat ggacaatgtc tttaattttt aatacatgtc
    14881 gcccattcca cctggcggca ttgccggtgc ggctggttct ttttccggca cctcggttat
    14941 tacagcttca gtagtcaaca tcaatcccgc aatacttgcg gcattttgga gagcgatgcg
    15001 ggttacttta gttggatcta taattccggc tttatacata tgaacatatt cttctttata
    15061 tgcatcaaat ccgaaatcat ctttcccttc tttaattttc tgggcaacga tagatggttc
    15121 atgaccggca tttttggcta tctggcgcaa aggctcttcg agagcacgtt tgataatttt
    15181 tacgccaacc atttgatcgc cagtaacttt aattttatcc agagcaggaa tgcatctgat
    15241 taaagcaacg ccaccaccag ggactatacc ttcttccact gcggctcgag ttgcatgcag
    15301 ggcatcttct accaaagcct tgagttcctt catttcaact tccgtcgcgg caccgacttt
    15361 caggacagct actccgcccg agagtttagc taagcgttcc tgaagttttt ctttatcata
    15421 atccgatgta ctggcttcaa tttgagcttt aatttgtttg attcggcctt tgatatcctc
    15481 gggtttaccg gcaccttcga caatcgtagt attgtcttta tctaccacga ttctcttcgc
    15541 atttcccaga tgtgagatag tagcattttc caatttgtaa ccggcttctt cagatataac
    15601 tttaccaccc gttaatactg caatatcttc taacatttct ttacgacgat cgccaaatcc
    15661 cggggctttc accgcggcaa cttttaaagt gcctcttagc ttattgacaa ttaaagtggc
    15721 taaagcttcg ccttctacat cctctgcaat tatcagtaag gaacgcccca tctgcgacac
    15781 tttttccaaa atcggcaaca attccttcat attgctgatt tttttgtcat aaatcaacaa
    15841 ataaggatct tctaaaactg cttccatagc ctcggcattc gttacaaaat aggctgaaat
    15901 atatccgcga tcaaattgca taccttcgac caagtccaga gttgtcagag tacttttggc
    15961 ttcttcaacg gtaataacgc cttccttacc gactttttcc attgcctcgg caattaatac
    16021 tccaatcggt atttcgacta tatcctcttt acctgattca tcttttatca tccggcggac
    16081 tttccggtta ttagctgaga tagttcctac ctgagctatt tcttctttac tgtttgtcac
    16141 agctttgctc aattctttta ggctttcgac taccgtattt acagccgtgt caataccacg
    16201 tttcaactcc atcggatcgg cacccgcggt aacgttcttg atgccttcag caataattga
    16261 ctgggctaat acagtagctg tggtggtacc gtcaccagca acatctgaag tttttgatgc
    16321 aacttctttc accatctgag cgccgatgtt ttcaagttta tcttccagtt cgacctcttt
    16381 ggctacggta acaccgtctt tggtaatcgt cggagaacca aatttctttt cgataaccac
    16441 attacgtcct tttggaccaa gggtaacttt cacaacatta gccagtttat caacgccctc
    16501 ttttagagca gttctggctt gtatttcata agtgatgtct tttgccatat ttgactcctt
    16561 ttgttagcat taaagtattg caaaaatgtc gctttcgcgc attatcaaat gttcttcacc
    16621 atcaatcatt acttctgtac cagaatattt tccatacaaa acatgatcgc cgacttttac
    16681 ttccagtgga atttttttgc catcatcagt tgtttttcca gggccaacag ctattactgt
    16741 gccttcctgt ggtttttctt tggcagtatc aggcaagtat atgccacctg gagttttctc
    16801 attagaaact aggggcttaa ctaagacgcg atctgctaat ggtttgattg ccatagaatt
    16861 tctcctttca attaatttat taattattga ttaaccaatt gtttttagcc attattctcc
    16921 ttcaagcatt caaagagcgc atttaaattt acaaatgacg tgccagtttt cggctcggac
    16981 agaaagtcag agaaaaatat ttcagctgac tgtaaccata gatagattat ttctagtaaa
    17041 atccttttaa tgtaaatttt gttttaattc tttcgtattt tcctatgacg attatgatcc
    17101 tgatatgata agttcaaatt tcctttttat tctggtggga tttgtggctg gtgttctttc
    17161 cggcttgttt ggtataggag gtggtattgt aattgttccc attatgattt ggttctgtca
    17221 tttttctcaa ttagccgcaa acagcatttc acttacggct ttacttctac cggttgggat
    17281 tctggccgtt ttggcttacc ataaatccaa gttggttaaa gtacgccagg ctttatttat
    17341 aagtttggga atttttatcg gcgtggttgg cggcgctatt ttagctcttg agattcccgc
    17401 tcatttcctg aaacgactct atggcatttt ccttttatat attgcttgga cttatattga
    17461 cattccatca ttatttgcaa atggagctaa ccaagagaaa actaaccagc aatttgaaaa
    17521 tttaaatttg gatcgaccgg tttgttatat agtgctggga tttgcggcgg gaataatgtc
    17581 cggcttattt ggcatcggtg gcggtattat aatcgtaccg gccttgatct caatttttaa
    17641 atatccatat aaaaaagctg tcggtacatc attgatggct ttacttttac cagtaggact
    17701 gccaggggtg ttgatttata atagcgccgg acaattacaa atcggtccag cgctatttat
    17761 tgctctcgga ttattatttg gtgcgttagt tggagcttta gttgcaatcg agttgcctac
    17821 taaactaatt aagagaatct acgggggatt tttattgtta gcaggcttta attttatttt
    17881 ttaataaaat caagacgata ccgctaaagc ggcttctaat cacggtttaa cgacggttac
    17941 tttcctcgat tttagaagct ttgccccgca aacctctcag gtaatataac ttagcccgac
    18001 gaactttgcc gacgctgatt ctttttattt tcgatatttt cggagagtgg agtggaaaaa
    18061 tcctttctac tcctattcca ttggagattt ttctgacggt gaaagtctca ctaattccac
    18121 tgccgtgacg agcaatgacg actccctgga aagtctgcag acgttcttta tcgccttcaa
    18181 taatcttgac ttcaaccgcg acggtatcac ctggtttgaa ttcaggaata tcagtcctaa
    18241 actgatccgc aactgcggcg gttaatttat ccattatttt tatcctccat attcatattt
    18301 ttaattaaat caattcttct ttggcgagtt ctaagtttgc gttgttcctg gcgccaggcg
    18361 tttattttgg cgtggttacc cgtggtcaag acctctggaa cacttttccg tcggtaaacc
    18421 tgtggatggg tatatattgg gccttccagt agattatccg taaaggaatc ggtctcagcc
    18481 gactcatagg aatggagtac ccccgctatg tgtcggataa ctgcatcaat aataatgagc
    18541 gccgggagtt caccgccggt caacacataa tcaccgatac tgatttcatc agtcaccagg
    18601 ttagcgatta ctctttcgtc aacacctttg tagtggccgc aaaaaaaagt caagtctgac
    18661 tcctgagcta attcgagtgc taaatgatgg ttaaacaatc ttccctgtgg cgttggaaac
    18721 acgacgcgcg gtcgtttatg cccagtcagt ttgacaattt tatcataagc acgaaaaaac
    18781 ggctccggct ttaagaccat tcccgcaccg cctccgtagg gtttgtcgtc aatctgttta
    18841 tgtttatcac gggtgaaatc ccgtaaattc cagacattaa attcaacctt accattttcg
    18901 acggctttgc ggaacatgct ttcagataaa caggctgtaa tcattcccgg gaatgcggtg
    18961 attatgtaaa ttttcatttc ggaaataaac cttcgatatc tttaaaaata acaatttgtt
    19021 gttcagggtc aatctgttca attaaatcgg ttactgccgg aacaaggagg gtcgcgtctt
    19081 ccgattgaat gacaaataat gcctgcggat tggtttcgtc caatgaacta ataaaaccga
    19141 tatctttggc tgaattgatt actctaaccc tgaatccgat aagctcaacg agctctttct
    19201 tcaattcata ttctcgtgga acgaaaacct ttagtcctct aagataatcc gcttcctgcc
    19261 gggaattaat atctcgcaat ttcaaataaa cttttttagt acttattctc agataatcaa
    19321 ccttccatgg gcgagctacc tgagcacttt caccgagcca aagtactttt ggctcatcta
    19381 cttttatatt tttcagagag ccgcgattta aaaggactgc cccgttcaga ccaaagcttt
    19441 ttaaaatgga tccgaccggt aacagtacca aatgatccat tttaacgctt attcaatgat
    19501 ttctaaaatt gcccttttct gaccttgttt ggcggcggcg gcagtcaaaa gtgttcttat
    19561 cgcgcgagcg gtgcgccctt ttttcccgat cacttttcca agatcacctt ccttgactga
    19621 aagttccaga attacggttt tttcactatc aacctgcacc acttttacat cttccggatg
    19681 gtcgactaat tgttttacga tgaattcaac aaactctttc atagttgtca ctcctctttt
    19741 ctagtttcag acaatttcaa tactactact caaatctact ttaaaattta gtaataaata
    19801 gtcaaaatat ctttaagact taaaatttca cgtttcggtc tggaaatcag gtttagattc
    19861 ggttacttcg ctggaagttt cactnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19981 nnnngggaca gattcgggtt ctgggacctc gacggcttgt gcttctttaa cttcttcagc
    20041 gatttcagcg cttttattat gagctaactg ccatttttgg atttcgatat ttcttacctg
    20101 ttcgctgata ttattgcgaa tcaaatgcca ctccattgca ataccttctt tctgtaatag
    20161 attgaaaaca gtatctgaag gaacagcacc tttttctaac caactaaaaa ccttttcctt
    20221 atctatttta acggttgccg gtttggtcag cggatcgtaa taacctaatt tgtcgaggta
    20281 tctaccatcc cggcgacatt tactgtccat tgccacgatc cagtaaaacg gtttcttctt
    20341 tctacccatt cgttgtaatc taatttttac tgccactaag agcctccttt tatttaatta
    20401 cctattccaa aagcagataa attcttgggt agtttcatcc gacccatttg tttcattaac
    20461 tttgttatct gccagtattg attaagtaga cggttgacat cctgcggctg agtaccactc
    20521 cccagagcaa tccgtcgtcg ccgactacca ttaattatat tgggtttaag ccgttcggtt
    20581 cgagtcattg attgaataat cgcttcggct ttaatcagtt ctttttcatc tactttttga
    20641 tttccgagtc gcatacgggc aaagccaggc aagtgttcca ttacgctatc aagcggtcca
    20701 agttttttta actgtcgtag ctgagtcaaa taatcttcga gggtaaattg ttcttttttt
    20761 aatttatttt ctaatttcaa cgccgattca aggtcaacgc tcgcttgcac tttttcgact
    20821 aatgagacaa cgtcacccat cccgagaatt cgcccggcta cccgctccgg atggaaaatt
    20881 tccagatcgc cgatcttttc accgacactc atatatataa tcggtttccc ggtagaagaa
    20941 acgatcgtca aagcagctcc accgcgcgta tcgctatcca ttttggttaa tatgattccg
    21001 tcaacaccaa tttgttcgtt gaaagaagta gcggtgttga ctgcgtcctg cccggtcatt
    21061 ccgtcaacaa ccagaaggat attatgcggt ttgatggtat ttttgatagt ttttaattcc
    21121 gccatcattt tttcatctat gtgcaaacgc ccggcggtat caattattag cgtatcgcga
    21181 tgttgctgtc gtgcaaaagt aattgcatct tggattcgcc cgactacatc ggggttctcg
    21241 cgaaaacagg gaacttttat ctgtcgagca agaatctcta actgctcttc agcggcggga
    21301 cgataaacgt ccacaggcac caataccgga ctatgttgct tctgaagtaa ttgataagct
    21361 aattttgcgg cgcacgttgt ttttccgcta ccttgcaaac cgaccagcat aatgatagtc
    21421 ggcggaatag aagcctcctt caaattgcgg tttttatctc caagcaaatg aaccatctca
    21481 tcatagatga ttttcacgat taattgtccg ggagacaggc ttttggcaac cgttaccccg
    21541 actgatttct cctgtaccga ctgaacgaat tcttttgcaa ccttgtaatt gacatcggct
    21601 tcaagtaaaa cccgcctaat ctgacgcatt gcatccgcga tattattctc agttatccgc
    21661 ccttctccac gaagggattt tatgatcgca ccaaaaccgt tttgtaattg ttcaaacatt
    21721 gtttaattca aattcattaa agcgggtaaa tttaattttt ttaacttctt tttgcaagat
    21781 attatcctcg caggattata tagtatcaag atatggtatc caccgtttca tcactaaaga
    21841 atataaggat ttatttgaaa ataaaggaag attgagttac cttttgcttg tgaatgctaa
    21901 ttaaaactaa aggattatca tgaatagaag acatctaatg atcgttttga cgttcccaat
    21961 attcttaatg tataattgct ccgaaacaca acctgagtcc agcaatccct accgaataaa
    22021 ttcgggactc aatcctggaa taaatattgg taatgctctc gaagctccga acgaaggcga
    22081 atggggcgtg gtgattaaag atgaatattt cccattgata aaatcggccg gattcaattc
    22141 cgtccgttta ccgattcgct ggtcagccca tgctgatatt aatccacctt atacgatctc
    22201 ggacgatttt atgaaccgag tgcgtcacat tgttgacact gctcttgagc acaaattata
    22261 tgtagttatc aatgttcaac attttcagga aatttacact gaacccatag aaaataaacc
    22321 gcgcttgctg gctttctggc atcaaatcgg cttagcattc aaggactacc cgcaaacttt
    22381 acttttcgag ccactcaacg aacctcattt caatctcacc cccgaattgt ggaatgaatg
    22441 gatacctgaa ttaattgata cgattcgggt cacaaatccc aatcgcactt tggtaatcgg
    22501 aacagccgac tggggtggaa tctggaaaat gaaagaatta aagttaccca ctactgaacg
    22561 aaatctaatc ctcgcaatcc actactatga accattccgg ttcactcacc aaggcgccga
    22621 gtgggaaaaa gaaagtaatg cctggctggg gcgcacttgg accaaaacca ccgatcaaat
    22681 gcaagaactc aaccagcatt tcgatctaat taaaaaattt gctgactcgt taggagtacc
    22741 ggtttatatc ggagaatatg gctgttataa tcgtgctccc gaagaatctc gattaatttg
    22801 gacacgaaca gtctccgaac aatgtgaaaa atacggattt agccgtgcct actgggaatt
    22861 ctgttccagt ttcggtattt atgatccgat cagtcaacaa tggcgggagc cgcttcgttc
    22921 agcagtatta ttggcgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa
    23041 tttgattttt atgataatta acaaacgatt ctgcgctaaa ataatattca cttcattttg
    23101 tctattcagt gctatgcttg ttcggctcga ggctgtcgaa ttgacaattt taagcgaaac
    23161 tacttcagag ctgattcttc gggtcgaaat ggatgccgac ttagaatggc gccaaataga
    23221 aaattacccg aataaagtca gcgccttgac tttaggagac gcctaccaat cccatttgag
    23281 tcaaaccctg gaaattcctt actatcaatg gctaattgca cttccgactg aggataaacc
    23341 ttcagtatcc ataaccaaat ctgtttttga gtcagtcaaa ctcttaaaaa acctgactac
    23401 ggacgagtta acggctctcg ataaatttcc cttggttgcg atcgccgaga ccggtttttt
    23461 aagagatgtt cccttcggtt cgcttgtaat atatcccctg cgttcagcaa ataattccaa
    23521 tgaattacgt ttattgcgcg agattgaaat tacaatacag tacacagcag taagaaaaac
    23581 gactatcaat cagactacgg tagaaaaggg cgattattta aatgcttttg tgaatggatc
    23641 atcagccaca caatggcaac aatctcgccc acttagtcta cggaaacctc agagccaacc
    23701 tgccggcaag tggtttcgca tcacggtaaa aaaagatggc gtttacaccc taaccctcga
    23761 tgacctaaaa agcgccggat tgactgagac aagcatagat acgggacgta tttatctcta
    23821 ctctaattcc accggtgggc gtcctctgga tgcaacaatt ggcgccgccg ttccatcgaa
    23881 cttaatcgaa aatactcgct ggataaaagg taataacgac caagatttta ctaacgctga
    23941 tacgattctt ttttacggtc gggctacaag cggagttgac ctcgattcta aaaactccct
    24001 cactttcgtg cgaaatcctt actcgctaat aaattattat tggttattca tcgccgatga
    24061 gcccggctat cctaaggcta tgccgactat aaatagtctt tcagcgactc ccaatatgac
    24121 cgtaactcaa agtgagcatc tcgaggttca cgaagaagaa aaggttaatt ttttacgctc
    24181 cggattaaaa tggagcggag aaaaattcaa tacctcgggt tcaaatgttt cggtaattct
    24241 gactatgcca aaagttcgtg atagttacgt tggtgtaatg acaatctgta ctcgcggagc
    24301 ggttgatgaa attttacatt attttaaagc ttatttgaac aatagtacga cgccggtcgc
    24361 cagttggtca gccactaatt atcaaagtgc cactacttct ttttcaacga ctctttcagc
    24421 taacataaat attttgaaaa ttaactatac ttcccaacct agtagcgcca gcgcttatct
    24481 ggattatgta atgtttcgtt atactaaaga cctaagcgca ttcactgaac cgcaaacttt
    24541 ctggtcactg ttgtattctg gaatagtcga atatcaaatt accgatctaa attttaccaa
    24601 tctactgatt ctggacgtta gcgactggag caatgtctcc atccagcaat acaaccgaaa
    24661 ttctaaccta atttcattcc gtacagccag tcaatccaat cagcgtcggc agtattatct
    24721 taccgctcca tcttttttta ataaaccgga aaaaatcgag ttaatcccga atcctcaatg
    24781 gattacattg cgcaatataa ccagttccta cgattacatc attatcactg ccgatgattt
    24841 cctgaccgcg gctgagaaaa ttgccactct atattcacag gaactttccc ccggagaccg
    24901 tcttaataca ttggttgtca ctcaaaatca aatcctgcgc gaatttaatg ccgacatcac
    24961 cgatcctcac gccattcgct tttttctgaa atatgcctat gaaaattgga cacccgctcc
    25021 agcgtatgta ctgttacttg gcgacggcac ttttgattac cgcgccatcg aatccagcac
    25081 aggaaattat gtaatgacct atcaggttga accggctgta gaagattact ccggtttttc
    25141 ttcctatgct tccgatgctc gttttgtcta tattaaaggt tcggatcagg ttatggatat
    25201 ggcaatcggt cgggtgcctg tgagaactgc tacggaagcc ctcgattatt atgaaaaatt
    25261 aaaacagtac cttactgaac caatttatgg cgaatggcgc agtcggatca cgctggtcgc
    25321 cgatgatccg caacgacctt ataacaatga aaaatatcat ataaatgata ctgaatctta
    25381 tattgccaaa tatattccta aaagtctcat aaccgataaa ttgtatttac ttgaatatcc
    25441 agaagtccag gatgtatcca gtaattgggt aaaaaaacct gccgcgaccg aagccatctt
    25501 aaatcaactc aaacgcggca caactttgat taattatctc ggccatggtt cttccacagt
    25561 ttgggcgcaa gaagaagtac taaaaatgga ccgcgacatt ggtttaatcg aagccggaat
    25621 gaaattaccg ctctggatcg ccggaacctg tagctggggg caatttgacg acatcaatac
    25681 tatttgtatg ccggaagcct taatcaataa aaatagcgac ggagccatcg ctatccttac
    25741 accaactcga ccaactactg gtactcagaa tgccagtttc ataggcaatt tcatcaaaaa
    25801 gttatttcca agcggcgaaa tcagtcgaac tagacttggc actctaattc aaaatattta
    25861 taatggttct attgaaaatc aggagaaata cgttctattc ggagacccag ccctttacct
    25921 ggcgttaccg tatcaaaaag ccgctttcga taaaatgccc cgcgacactt tagcggcatt
    25981 ggaaaaggtc agcgttaccg gacgaacagt taccgaagag aatttcaacg gtgacggtat
    26041 tatcaatgtc tttgacagcg atcgctacgt aacccgctat tacctcgacg acgctaaagt
    26101 aacccagaat cttagttaca ctctttctgg tgaaacgctc tatcgaggca atcttaaaat
    26161 cgaaaatagt aaatttttct cccgcttcta catcccgaaa gacctcagtt atatgaacct
    26221 tttcggccgg attaaggttt acggctggaa cacttctgct atgagtgatt ttgccggttg
    26281 ttatgattca ttattattta ccggtaccac ccaattcgat gataccagtg gcccgcaaat
    26341 cgtgatcggc tttgaaggta tggattttaa aagtggagac atcgtcgctc ccgatgccaa
    26401 aattaaagta tcaattatgg atccagtggg aataaacatc gccggcaaac ttggacacga
    26461 tatcactttc cgttttgata atgataatga ccttattttt gaaatgacgg aagactttgt
    26521 ttatgatgta aatagcgact cttcaggcgt tatcaccagt gacttgcctg atctctcacc
    26581 gggaatccac caaatcactg taaaagcctg ggataatgct aataattatg caactgccat
    26641 agctgaattt gtccttgcca gttctgataa attgaaattg gaaagagtag tcaatttccc
    26701 caatcccttc gctagtacaa ccgactttgt cttttatctt actcagccgg gacaggtaaa
    26761 aatcaccatc tacaccatcc gcgggctaaa aataaaaacc atagcgagcg atgaaatcct
    26821 accacctggt ttccatcaag tgaattggga cggatgtgac gatttcggtg atgaaatcgc
    26881 ccgcggcatt tacctctata aagttcaagt acagtcatcc gattcaaacc aaaaagatac
    26941 cttcatcgga aaaatggtga aatcatgacg acccaactca attggtattc tatgccgatt
    27001 attttggctt ctcaatcgcc gcgccggaaa gaaattttag acatgattgg actgaaatac
    27061 gaagtctgtc ctccccatta tatcgaaaac aattcaattg gcaagtcgcc gacaaccatc
    27121 gttataaaac acgccctgaa taaagcccga accgtagcac ctcgctattc gaaagcttgg
    27181 attatcggag ccgataccgt cgtcgtttgt aaaaacagaa ttttaggaaa accacagaat
    27241 gaaaacgagg cggttcagat gttaaaattc ctgagcggcg tcgaacatag cgtcttcacc
    27301 ggatattgcg ttctgaattc cgcaaatggt cgttatttga aaaatgcagt gcgaacccgg
    27361 gtcagattta aagaaattcc tttggcggaa atccaatatt acgtcgaaaa ttatcatccg
    27421 ctcgataaag ccggggctta cgccattcag gattttagcg ctattttcgt ggaaaaaatt
    27481 aacggttgtt tttacaatgt tgttggtttt ccactaccgg ctttctatga acaggttaaa
    27541 aatgaacttt ctatctgcct ctgaaagttt ttattgaaat taataggaat ttttctgata
    27601 tttgcaacaa tggaaatgaa cttgaggaac aatggcaacg ttttatgaaa tattaaaaca
    27661 aagcagagaa gcacagggac ttaccgttga gcagatttcc agccggacca aaatcagcaa
    27721 agaaataatc acagccatcg aatccggtga tctaaaaata ttgccgaaaa catatatgcg
    27781 tttattcctg aaagcctatt ctcaggaatt aaatttagac cctgaagcaa ttcttaaaaa
    27841 tttcgaagaa tcgctcggcg aaccagttga gacaccggca gttccagaca acaacattaa
    27901 gccgagttta cccatttcac aaaagccgga aaaaccagaa aaaccaataa cagtaaaaaa
    27961 acaaaaccgc aatttttcta ctgtaataat cgtttttgta attctcgttt ttttgattgc
    28021 agtcctcaga caggttttga tggatgaaaa aaagacgagc agtaatactg cactaccgaa
    28081 aattactcca actgacacaa ctcaaactat tcctccgagt cccgatctcg aagtagcagt
    28141 tcccaaaagc gaattgaatt taactattac aactaaagac agctgctggg tgcggattgt
    28201 catagataat cgtacgactt ttgaagcaac tctgccacca ctctataaaa aagaaatgat
    28261 cgccaaagag caattcgata tgcaaattgg tcgccccgct tctattaatc tagttttaaa
    28321 tggaaaagac ttaggcccgg ttggcattcc tggcattcca acccgtttaa taataaccaa
    28381 agatgggatt attaagcgtc agagttttta atctcaataa cttttcctca ttttaatttc
    28441 tcctcttttt aaataaccca ttaatcttct taaaaccttt taaaaaaact cttgacaatt
    28501 agtttttttt gtcctatttt gtgggtgaat gtggtaagaa atcccaaata atcccaaaaa
    28561 tgcgaggaaa ctgtaaaccc gatgattact gcctttgccg gtgaaacccg ccacccactt
    28621 gacgaaaaaa atcgcttgag tattccggct aaataccggc gttgggctga tggtcaggac
    28681 ggcgagtgta cctttgtggt aaccaaaggc tcggatcctt gtttagttgt ctatccatcc
    28741 accgaatggg atgtattatc tgagaaactt ttgaaattat cctattttaa gaagaaaaat
    28801 cgggctttta tccgagcgct ggcacgtagt tctgttcgtc taaagtgtga cggccagggg
    28861 cgtattttga tacctacaag tttacttgaa tacgcccata ttaaaaaaga agtaacgatc
    28921 atcggagtat tgaatcgtct cgaattatgg gatccggaaa cacttgaaaa acatgaagag
    28981 tcttcgattc ctctcgatga tgaatattac gaagacctgg gcgacattct ttaatcttga
    29041 tatttggaaa attatggtat gactaccctc catattcccg tattggttga acaaacaatc
    29101 caatttttga tcaatagacc gacgggaatt tacgttgact gtacccttgg taccggtggt
    29161 catttccaag cgatagccca acatttagat aatgacgcat atctaattgg tatagatgcc
    29221 gatccgagtg ccgtgcaata ttgcgagcaa aatatttcga tttctcagcc tcaccagttc
    29281 atcactgcca attttgctga tattaaacgg atctgctatc gggcaggctt tccgaaagtc
    29341 gacggtattt taatggatct cggattatca tcatttgcct tagacaatcc cgagcgtggt
    29401 tttacttata gtcaggctgg tcctctcgat atgcgcttct cgcctgatac tgctcagact
    29461 gcaatggact ttatcaatac agcctccttg aaagaattgg tattagtttt caaagaatat
    29521 ggtgaagaac gttacagcca gaatattgcc cgtcggatta tagctgatag agataaaaat
    29581 cctattaaaa ctactggcca attagcctct attattcgag aagaagttcc aaagcaattt
    29641 caaattaaaa ccttgagtcg tatttttcag gcgattcgga ttcaagtcaa tcaggaatta
    29701 gacgccttac gacaaggtct atcagatgct gtttcattat tagccccttc cggacgaatt
    29761 gtcgttattt cttatcattc catcgaggat cgcatcgtca aacaattttt taaaaaggaa
    29821 gccagcgatt gcatctgccc tcctgatttc ccgatttgtc aatgtaatca ccatgcccag
    29881 ttaaaaattt tgactcatcg cccgatagtg ccgagcaaat ctgaaataga aagaaactca
    29941 cgggcacgga gtgccagact gcgtgccgcg gaaaaattgt agagatcaaa tatgtccagc
    30001 cgcaagtcat ttcgtaaaaa gaaaaaaaac ctactgttga taattggctt tttcgttctt
    30061 gtcggttcaa tggtatttta tatttggatt tacaacacca ctaacgctct gtatcgcgaa
    30121 atagaccagt taaaacgaaa ggaagccagt ctgattgccc gaaatcagat gctcgtcgtc
    30181 gaaattgaga gactgagtcg ggcagatcgc ataaaaaata ttgcgacaac cgaaattgaa
    30241 atggttacgc ctttaccaga aacattagca gttgttatag attctaaagt tttagcagtt
    30301 gaataatggc taatcaattt aaatccattc ggaaaaggac tttgatagtg agtagctcat
    30361 tagtggttct ctgggtggtt ttgatagcac gcctttttta tattcaagtt ttaaattcaa
    30421 aaaaatatca ggaattatgt aataatcaag ccaatctgcg caaagccgtg ttaccgcgcc
    30481 gaggcatatt ttacgatcgc aacggcgagg ctttaaccat tgatttagtt aattactcga
    30541 tcgcggcgca tccctacttg gttgaagata aaccccgttt agccagtact ctggctaatg
    30601 atttgagcgc caaacccgag cagtatctta aactgttgga atccaataaa actttcgtct
    30661 ggctggaacg cgaagtcccc tattcaagtt ttcaaaatta tgaaaaatat gaaaaaactc
    30721 ccggattggt tatcgaaaaa aatgttaaac gctactaccc actcggtgaa attaccggtc
    30781 aattgcttgg ttttacaaat atagataatc gcggagtttg cgggctggaa agcggattgg
    30841 aaggtttact ttgcggaacg cccggctgga ttatggttca aaaagacggc tggggacgac
    30901 ttcactatcg gccggatttg ccttccaaaa gcagtattga cggcaatgat attgtcctga
    30961 caattgatcg ggagtatcag actattttat atgaggaact tttacaggct tacaccgaaa
    31021 caaaagctga aaaagctatg ggcatcgtga ttaaccccaa tgatggcgat atcttagcta
    31081 tggttacctt gccacctttc aattctaata aaccttccga ttatccgatt tctgctcaac
    31141 gcaatgccgt agtttcggat atattcgagc cggggtcaat tttcaagatt gtaaccgcga
    31201 cagccgctct cgataacaaa gttctgcaac ctaaacacca aattgattgt aatcccggtt
    31261 ttattcaagt cgggaatcgg ataattcgcg atcatgataa atacagcatt ctgactttcg
    31321 cggaagtaat aaaaaatagt agtaacgtcg gaaccattaa agttgcccag ttgacaggaa
    31381 aagatcaaat ctttaactat gctcgcaagt tcgggtttgg ggcaaaaacc gatatccagt
    31441 ttcccggtga agaatccggg attttacacc catttaaaaa atggaacgac ctagttctgg
    31501 ctcaggttgc tatcggtcaa ggagtctgcg tcacggcttt acaaaccgga atggcttatg
    31561 cggctattgc caatggtggc ttattactta aaccgcaaat cgtcaaaacg atcaagacca
    31621 aagatggcat tgaaatcttc aatgctcaac cggtttatat caggcgagtt gccagtagtg
    31681 aaacaatgag cactttacgc gagttattaa gactaacagt aatgagtggt acaggttcac
    31741 gtgccgatat caacggatta gccattgctg gaaaaaccgg aactgctcaa aaagttgtca
    31801 acggagctta cagtcagacc gagtacgtgg catcgttcgt cggattcttt ccggcagata
    31861 atcctaagtt actgtgtgtg gttgttattg ataatccgaa aggcatcagg catactggtg
    31921 gtggtgttgc ggcaccggtc gttcgagaag ttttcaaaag aatcgtgaac cagtctgacg
    31981 atctgttctt cgatgaacca gctcccttac caaccccagt ggttaattat gtttacgaaa
    32041 ctaaaccggc ggtcgctaat gttcctcaaa cacctgacga ggcttatcat cccaatttaa
    32101 cgactgcgat ttataccaat caaatgcctg atctttgtgg aaaaactctt agacaggcgg
    32161 ttggaatttt gtatgaactc ggaattgacg atatcgaaat tgacggtgcg ggagtggtgg
    32221 tcagccagaa gccagcacct cattcgccaa tcaatccgca tgtcaaatgc atattgaaac
    32281 ttcaacccac tcggacgcct cttgagtaat tcactttccg aaatattaca aggtattgaa
    32341 tttcgtcggc tgggaaactt accgacttcg gtagcggctc ttagtgtcaa ttccaaggaa
    32401 ttggctaagg gggcgctctt tttcgcaatt cgcggggcta aggatgatgg ccataaatat
    32461 gttaaaaacg caatacaaaa cggtgcggcc gctgtggtgg tcgaagaagt agtcgctgat
    32521 gaatccatcc cccagatcat cgttgccgac actaaaacag ccctctcaaa agccgccgcc
    32581 aatttttatg aaaatccgac caataatatg acgatcgttg gagtgaccgg caccaacggc
    32641 aaaactacca ctgtttacct attaaatgaa atattcaaag aagccggtct aaagcacggt
    32701 actgtcggca cgctgggtta ctcgattgga gatgaacgtt tctcctcgaa cttgactaca
    32761 cccgatagta ttcaattaca gcaaattttt aaaaaaatgc tcaatcgcgg agtcgaaata
    32821 gcggctatgg aagtgtcttc gcatgcttta gccttgcatc gcgttgacga tgtccatttt
    32881 aaagccgccg tgtttaccaa tatttctcaa gatcatctcg attttcacca tagtctcgat
    32941 gaatatgcca caactaagag caaactcttc gcaatgattg aaaaagacgg gtttgtaatc
    33001 aataatctcg atgataatta ttccgattta ttcaagaacg ccgctcgatg ccgcctgttc
    33061 acctattctt tgctgaagac agccgatttc acctgggcgc ccggagtaac ctggtcaaac
    33121 ggcatttctg gggaaatcct aactcccaac ggcaaaattg aaatcaagag ttgcctttcg
    33181 ggacaattta acttgaaaaa tattttggcg gctacggcgg tagcggttaa tctaaatatt
    33241 gacgataaaa tcatcgcttc agccctagcc agagtcaaat ctgtgcccgg ccgcttacaa
    33301 gaaatttcgc atcctggcca atttcgcgtt tttatagatt atgctcatac cccggacgcc
    33361 attactaatg tcttgcagac tctacgtgga atgcttccgg ccgacgggcg cttaattgta
    33421 gtttttggtt gtggtggtaa tcgcgatcgc gataagcgtc ctaagatggc gcgagccgtc
    33481 gagtccttag ccgacttggc gatcgtcacc accgacaatc cccgcttcga agaaccgcat
    33541 gatattatta atgatattgt taagggcttt acgagcgggt ataatttcaa agtaatcatt
    33601 gatcggaaag aagcgatcct ggatgcttta aaaaatgctg agcaaaacga tataatcgca
    33661 attcttggca aaggtcatga aacctatcaa gaaattcgcg gagtcaggta tccattttac
    33721 gacggggaaa tcgtacaaca attctacggc ggatcaaatg attaaggcag atttcattcg
    33781 gtcactcggt ctcggacgca tagtcaattt cgaaggggca atcaatggcg cggaaattga
    33841 cagtcgcaaa cttcagcctg gtgcggcttt cttcgcttta aagggtgcga catccgatgg
    33901 tcatgcttat gttcaggagg ctttgcagaa aggtgccgtt ttgtgcgtgg tagcggaaga
    33961 ttgggcggct aaaaatccga ctcccgcacc tctctggatc gtacccgatc cggaaaaagc
    34021 cctccagaaa ttagccgaaa aatggcgcct ctccttctcc attccggtgc tggcgattac
    34081 cggaactaac ggaaaaacga caactcgtgc gatgtgcgcc gcaattataa aaacaaccta
    34141 taaattgcat acaacttccg gcaatttcaa taatcaactc ggtttgccga tcactttgtt
    34201 gaatttaaga cctgaacacc aattcagtct aattgaaatg gggacaaatc atttcggcga
    34261 gattgcccga ctatgtgaaa ttgcccatcc gaatgccggt ttaattacca acgtcgggtg
    34321 gggacatatt gaatttttcg ggaatatcga aggtgtagcg caagcgaaat cggaattgtt
    34381 tgccgccttg ccggaaaatg gcaccgcttt tattaatgca gatgatcctc taattgtcaa
    34441 aatgccggta gccgggcgca aaattaaata cggttttgaa aataaagacg ttgatttcta
    34501 tggcaaaatc tcaggctata ataatgaagg tcaagcggtt ttaaacgtca attcgcggtt
    34561 cgacattcac ctgtcaattc ccgggaaagt tgccgcctta aatgctctgg cggcggttgc
    34621 agtcggttta ttttttaatg tagatgaaaa agcggttata gcggctcttg aagcatttca
    34681 accggtcagt caacgctttg tgcagattaa agtcggtccc tatcaggtga ttaatgatgc
    34741 ttacaatgcc aatcctgatt cgactatcgc cgctttggaa acctttagac ggattaaaac
    34801 ttctggacga cgcattttca taatgggcga tatgctcgaa ttaggtcaat atgctattca
    34861 agggcataaa cgagtcggca aggcggtcgc tcaagccgat attgaccgct tttatggcgt
    34921 tggaacgcta actgcctatg ccattgagac tgccgctcgg gctggcttaa aagccgctta
    34981 ttatttcagc accaaatccg atttattagc cgctttaaaa tccgatttga aagaaggcga
    35041 tactctactc gtgaaaggtt cacgcggcag tcgtatggaa gaaattattg agggactaag
    35101 ccaataatat gttttaccat ctcctctatc cattaaaaaa atacttcaca ggatttaatc
    35161 tgtttcagta catcaccttt cgagcggcta tggcggctat tacggctttg ctgattagtt
    35221 tcattattgg tccatacatc attaaaaagc taaaaaaata ccaaatcggc gagttgattc
    35281 gggaagatgg cccccagaca catttggcta aaaaaggtac accaacaatg ggcggaatta
    35341 ttatcttatt ttcggtaatt gttccagtac ttttgtgggc taatcttacc aacacctaca
    35401 taaatctaat tttacttagc acagtctgga tgggtatttt cggtttcctc gatgattatt
    35461 taaaagtcgt taagaaaaag aaaaaagggc tgatcgctcg ttataaactt ttagggcaaa
    35521 tcgctctggg cttgttaatc ggttgttatc tgtatttctc acctgaattt gctgacatta
    35581 actcaatcac ttccgtaccg tttttaaaaa ataattatct tgatttcgga tggttttata
    35641 ttccgttcgt aatttttttc atcactggta tttctaacgc cgttaatcta actgacggcc
    35701 tggatggtct tgccgctggc ttgatgggaa tcgttgcctt ggcgctggcg gtcattgctt
    35761 atataaccgg gcgcgtagat tttagtaatt acctgaacac gatttatttg gcgggaagtg
    35821 gggaattatc aatattctgt atggccgccg ccggagcatt tcttggtttc ctgtggttta
    35881 atgccaaacc ggctgaagtt tttatgggcg acactggctc actttcaatg ggtagcgctc
    35941 tggctactct ggcaatttta ttgaaaaagg aaatattgtt catttttctg ggcgctttgt
    36001 tcattgccga ggcgctctcg gtcataattc aagtggttta ctttaaatgg actaaaaagc
    36061 ggaccggcgt tggtcaacgg gttttcaaaa tggcgccctt acatcaccat ttcgaaatgc
    36121 agggcatacc ggagagtaaa attgtaatcc gcttttggat aatcggcatt ttgttggctt
    36181 tgttcagtct aagtactttt aaaattctat gatagattta aaaaccaaac cagctaatct
    36241 gaatgattta aaagtcgccg ttctgggcgc taaacgcagt ggaattgcgg ccgcaatatt
    36301 agccgcccga cggggtgctg aggttctttt atcagatacc gccaatccgg aaattgcccc
    36361 agaacagcat tccgcattaa agcaacacaa tgttcaattg gaaattggcg ggcacacctc
    36421 gaaaattttt gattccgacc tagttgttct aagtccggga attcccaatg atgctcccat
    36481 cgtcattgaa gtcaccaagc attcaatacc gatcgtggct gaagtggaga tggcttactg
    36541 gttctgccct acggataata tcatcgccgt tactggttcc aatggtaaaa ctactaccac
    36601 tacccttcta tctgagattt ttaaaggcac tcaatacgaa ccatattgtg gcggcaacat
    36661 cgggacggct tttagccatt tgatcattga ggctgaacgt tccgcccacc cccagaaaat
    36721 tttcattctg gagctgagca gttttcagct ggagcgcatt gtccatttcc gtccgaaagt
    36781 ttcgctgatc ctgaacataa cgcccgatca tctggatcgc tacgaacatc gtatggagct
    36841 ttatttagcc gccaaattgc gcataacgat gaatcagacc gaagatgact actatatttt
    36901 taatgacgat gatgaattgc taaagactaa tttgccgccg agatgccatt tgattcccgc
    36961 cggattacat tccacttccc agaaattaat tacttcagac gccgagaaga tttatttttc
    37021 cggacaagat caattcattg aacgggcgcg agttgctttg ctcggcgaac ataatcttta
    37081 caatttgacc gccgctttga ctgccgggcg attgtatcaa ttaccaatta cgcatttaca
    37141 aaatgtattg tacaatttca aaagcattga acatcgcctc gaatttgtga caaccataga
    37201 cggcgtggac tattacaatg attctaaagc cactaacgtt gattcggtca attatgccct
    37261 aaaaagtttt aaccggccgg taattgtcat tttgggcgga aaagataaag actccgattt
    37321 tacgcttctc atcccgcaaa tccgggaaca tgtcaaagcc gcagttttaa ttggtaaagc
    37381 cgctgataaa attcgcaagg cattgagtgg tgtcgtcgag atgtatgacg ccgggtattc
    37441 tatgccgaat gccattgaaa ttgctcgcaa tctggctgtg cctggtgaca tagtcttgct
    37501 ctcgccggct tgtgccagtt tcgatatgtt taatgattat gaacatcgcg gtcgggtttt
    37561 taaggaaatc gtgatgaatt tgccgaagga cttgaaagta tgaacgttgc ttttaaaaaa
    37621 ggaaaagttg accaaaaata cttgatcgcc ttttttattc tgttaggttt cggtttagtg
    37681 atgcaatata gtgccagttc ttcgcttggg gaagcccgtt ttgacgatcc gtctttttat
    37741 gttcgtggac agtttattcg gattgtgttg ggtatggttg ttggtcttat ttgtactttt
    37801 atcaattatc aaattctcaa aaaaggcgcg ttctggattg ctctggctgg gattattgca
    37861 ctgattgcga ctttagtcta tcacaaattg caccccggtc aagcgactgc gcgctggttt
    37921 ccgatcggcg gttttaattt tcagccttct gaattcgcca aattggcatt aattatttac
    37981 ctttcatcct tcatcgagcg ccacgaaaga catctggatg atttcaagcg tggttttata
    38041 ccaccagtca tcattatgtt aataatggtg atattgatta tcgtcgagcc gaattacagc
    38101 acagctgctg tttttactgt tttcggtcta attgtcttat ttatcggtgg cacgaaactg
    38161 ctccactttc taccctttat aggtggattt gcattattat cagtggttac cattctgcgt
    38221 tcaccatata aactgatgcg catcctgact tttctagaac cggaaaagga tttacagggc
    38281 gccggttatc aaattcatca atcaatgatc acactgggta atggtggttt tttcggtcgt
    38341 ggtctgggcg gtagtatcga gaaaaatttg tttttacctg atatgcatac tgattttatt
    38401 ttctcagtag tcggcgaaga gcttggtttt atcggggcgg tggttttgct atcactttac
    38461 ctttatctat ttatcagggg aataaaaata gctctgcgcg cgccggatgt tttcggcaat
    38521 ttgctaggta tcggtctatc gagttgtttg tttatttacg taattgttaa tgtgggtgtt
    38581 acctgtggac ttttaccggt aaccggcttg cctttaccct tcataagcta cggcggttct
    38641 tcgatgctat tcaatttcgc ctgtgtcggg atcattctaa acatttcacg ctatgcggtc
    38701 ccgcaagaaa aaacaaattc actggtgatg ttcaatgact aagaaagatg aatctcgacg
    38761 tttaaaagta ctaatcacgg gcggcggtac cggtggtcat ctttttccgg cgatcgccct
    38821 ggccgaagaa cttcagatgc gtcaatcgca gattggcgaa ctcgaaatca tttttattgg
    38881 aacgcggcgg ggcttggaat ctcaaatagt tccgagtctg ggttttgaat tccataaaat
    38941 ttggattcgc ggctggcaac gcggctggac tctaaatgat attttggtca atctgttatt
    39001 tcccgtcaga ttattcgtca gtttgataca atcatttttt atcattaaaa attttgatcc
    39061 cgatttagcc attggaaccg gcggctatac ggctggacca cctctaagaa ttgccgcaat
    39121 gctgaaaatc ccgatttttc tccacgagca gaatgtcatt ccaggggcta cgactaaaat
    39181 gctggcaaac aaagccaagc ggctttatac gtcttttgaa gagtcaaaaa cttacctgga
    39241 aaacgccatc tgcctcggca ctcctctccg gcgttctctg caaattgttc cgcgggagca
    39301 ggcgatttac ttttttgaat tggagcccga tcgcaagacc gtttttattt ttggaggcag
    39361 tcagggctcc caagctatta attcttattg gattgagaat atccccacgg tggtggaagc
    39421 cgttaaatgt caattcatct ggcagaccgg acagcgcgat tttaaaatga ttgataacat
    39481 ttttagtaac aatccctggg ttcacgtcac gcctttcatt catgaaatgg gaattgccta
    39541 ttgtgccgct gatgttgtca ttagccgggc aggcgctcta tcgctggcag aactttgtct
    39601 ctatggcaaa ccttccattc tcgtgccttt accaactgcg gctggcaatc atcaggaaat
    39661 caacgcccgc aatatggaac agcgtggcgc ttccattttg gtactccaga aagaattgac
    39721 taacgaccga ttgactcagg aacttatcgc tttactgaaa gatgagccgc gtcttcaagc
    39781 tatgtctgcc aacgctaaag ccttagccag accggattcg gcaaaattaa tcattgacga
    39841 tattttaggt tttatcgaag taaatgcact ctgaagttaa aaaatatcac tttgttggaa
    39901 tcggcggcat aggtatgtgc ggtctggctg aatatttatt gcacaacggt caaattgtaa
    39961 ccggctctga cctggcgcct tccgaaaata cagaacggtt ggaaaatctg ggcgctcaaa
    40021 tcaaatacgg acatcgccgc gaaaacgtcg gcgattgtga ctatctggta tattcttcgg
    40081 ccgtcaagcc ggacaatccg gaaagaatgg tcgctatcga acgtcaaatt ccaactctgc
    40141 ggcgggcgga attgcttggc caaatctttg ctactaaacc cattcggatt gcagtcagcg
    40201 gtactcacgg aaaaacgacg acaacggcga tgatcgggca agtcttgact acggctggta
    40261 tggacccact cattttaagt ggtggcgttt tgaagagtag cggtagcccg gttcaaatcg
    40321 gcaaaggtga tatagtaatc gccgaagccg atgaatttga tcgcagtttc ctgcaattat
    40381 ctccaacgca tgccatcatc acaaatattg agagtgaaca tttggattgc tatgcaaacc
    40441 tcacccaaat caaaaatgcc ttttatgaat ttaccgctaa gataccggat gctggcagag
    40501 tcatcgcctg ctcggatgaa aaagaaatac ccgacttttt aaaacatcat ccgcggccat
    40561 atgttaccta tggtctttat agcggagaat ttcaagctcg taatatccaa ttaaaaggga
    40621 attcaagtaa ctgcgaagtt tatcataatt cagatttaat tggctcgctc caattacacg
    40681 tccccggtct acataatatt aaaaacggac tagcggcgat cgcgatggct tctttactaa
    40741 acataccctt cccggttatc caggaaggtt tggcagactt tcggggtgta aaaaggcgat
    40801 tcgaaattct acaagaaaac gacaatctta tgctggtgga tgattatgcc catcatccca
    40861 gtgaaattgc ggcgactctc aacgccgctc gcaaaggctg gcaaagacga atcattgcgg
    40921 ttttccagcc ccatttattt tctcgtactc gggatttcta tcccgaattt gcgcgggtac
    40981 ttcgggtggc tgatgttatc gttcttcttg aaatttatcc agcgcgagaa gcacctttag
    41041 aaggtataag ttcccgcttg atttatgatg aaattcgcaa agaccggact caggacgttt
    41101 accttaccaa cacgctcgct gaagccctag cattattgca agaaataaaa ttaaccggcg
    41161 atatgattat cacactgggc gccggtgacg tcaacaaaat tttaccgaaa ttaatggaat
    41221 aaaaaattga aactgggatt gataatatta aaaaataaat cgcaaaggtg tttaggtgtt
    41281 cagaatttaa cttctttttt cagagagtac cgtgacttag atagactctg tgatcaaaac
    41341 ctttggacaa aaattttcca gggttcccgc agatatgggg ggcaaaacat caaaacgccg
    41401 gctcttcagc accgaaaaat ttctgcaatc ggttatccca cgaacaaaga tttcgatctg
    41461 aagacacagc aacgccaggt aattttgcta ttattctcgg gaaccctgac aggttttgga
    41521 ttaactaatt gatgattggt gcaatgacag cagaaaaagc aactaaaatt agtcggaatg
    41581 accaagccaa gcgtcccaga aaaaaagtat ggttttctct ctgtttagca ttgattggcg
    41641 gattcggcta cctcgtatat ttggctattg actggtgtga atatactaat ttctttaatc
    41701 tcgataaggt cgaagtcagc ggagtatcaa ttttatctaa tgaagaaata ataaaaatcg
    41761 ctggtcttaa gaaccacgtt agcctgctta aattagacat tcatcagatt cagaaccggc
    41821 ttgagatgga accatatatc gaggcggcag tcgtcagtcg cgaatttccg aatcacttga
    41881 aaataatgat caaagagcgc atcccagttt gttacttgaa tacgggcacg ctctggctaa
    41941 ttgaccaaca aggaatcata ttaccgcttc gcaaggaaaa attaaaagcc aatttgccgg
    42001 ttattaccgg tttcgagaaa gattccgccc gttgtcggcc gggacagctt gcacctaatc
    42061 ccgaaatgtt caaagccttg aatttcatcc agactgcggc tctgaaggcc aatactctgt
    42121 ataccgaaat ttcagaaatc tttgcctgga aaaagggcga atttattatt tatacggtca
    42181 acggaggaac accgatttat atcggacgtg gtgatttaac caaacaatta tatattctgg
    42241 cggcttttca aaacttaatc aaatccaagc gcacttttgc cgattatcaa tatcttgatc
    42301 tgcgctggga taaacaaatc atagcgagag aacgaaaatc ataaatgaaa atgttaaaac
    42361 taaaaaacca aatcctgacc ggtctcgacg ttggctcatc taaagtctgc gccgtaattg
    42421 ccgaagttac tcccgattca cccgaaccta ttattaaagg gtacggtatc agtccttcta
    42481 aaggaattaa gaaaggcttg gtcgttgata ttgaaaatgc gactcagtca attgttaatg
    42541 ccgttagcga agctgaaaaa atggctggtt tttcgattaa ttcctgcctg gtaggagttt
    42601 cgggcgatca tatccgcagt ttgaatacac gtggaatgat ggcaatctcg cgcgattcac
    42661 gtaccggctt aggcgaagcc cgagaaatcg agatcgaaga tatcgagcgt gtaatcgaac
    42721 acaccaaggc tgtaccgctt ccgttagatc ggcagattct acatgttctt cctcaggaat
    42781 acatcattga tgaacagccg ggagtaaaaa atccgttgaa tctcagcggt cgacggctgg
    42841 atgccaaggt acatctgacc acctataata ccaccgttct ttccaattta agtaaatgta
    42901 tcaagaatgc cgatctggaa attgacggct ttgtattgag ttctctggcg gccgcctatg
    42961 cctgtctgga agagagtgaa aaaacgatgg gcagtgttct tctggacatt ggcagtggag
    43021 tcattgacat aatcgttttt tacgatgacg gtgttcacca taccggagtc gtcaatctgg
    43081 gagctaatag cgttaccaat gatatcgctt atatgctgag agtccccctc gaaaaagccg
    43141 aaagtattaa acgtgaatat ggttttgcca aagtctcttt gaccgataga gaagcatttt
    43201 tcagactgga tagtcttggg gggcggtcac ctcgagaaat ttctattttt aatctggctg
    43261 aatggattga acctcgtatg gaagaaatat tgcgcgaatc atatctcgaa gccaaaaaag
    43321 ccgatattcc tttgactaat acattatcag tcgtcctaac gggcggcggc gcccgtttaa
    43381 aaggcaccga tgaattagct gaaatgattt tcaatacccc agcaagagtg gcagctcccc
    43441 gtggtttcca ggaatatgtc acggaattaa gtgatccgat ttattcggtg gcaatcggct
    43501 taattaaata tgcaattgcc ggacaaatta gcggttcggc tttaaataaa acaatcagcg
    43561 gtccctttca cagaataagg ggctggatta aacacgtaac cgaaaatgta atgtaattag
    43621 ggaggcctaa aatgttattt gaatttgaag aattagtcaa tcagcaagcc agactgaaag
    43681 tggtcggcat cggtggcggc ggcggaaacg ccattaccag aatgattgag aatggactga
    43741 tcggggttga tttcatcgct ctaaacactg atgttcaggc gctcgaatgt tgtaaagccc
    43801 caactaaatt tcagatcgga cgagctctga cacgtggtct gggtgctggt gccattgatg
    43861 acattggaag aaaagcggct gaagccgatc ggcagacgat agcttcaatg attgaagata
    43921 ccgatatggt tttcattact gctggaatgg gaggtggcac tgggactggc gccgcaccaa
    43981 tcgttgccca aattgcacgg gaacaaggtt cattgaccgt cggaatagtc actaaacctt
    44041 ttaatttcga aggggcgaag cggatggcac gcgccgaaga gggtattaat caactcagga
    44101 aatgcgttga tactctaatc gtaattccga accaaaaatt attggcaatt gttgaccacg
    44161 atacttccat attggatgtt tttaaaactg ctgatagcgt attatatcaa gcgattcgtg
    44221 gcatttcaga cctgattaac aaaaatggcg ttattaattt agattttgct gatgtcagaa
    44281 ccattatgaa agatatgggt aaagccatta tgggtactgg tttggcaacc ggtgaggaac
    44341 gcgctgttct tgccgctcag caagctattt ccagtccact acttgatgaa gttaatatca
    44401 aagatgccaa aggtctgcta attaacatta ccggaccggc taacctcgac atccatgaag
    44461 ttgacgaagc ctgttcaatt ataaccgatg aggttggtgc caacgctgaa accatctttg
    44521 gtatggtagt ggacgaaaat tacaccgacg aaattatgat caccgtaatc gcgactggtt
    44581 ttggtgaagc gccgacgccg attaatctgt cccaaaataa aaaggatttc tcctccccaa
    44641 ttaaagacga accacgaaat actgacgata tttggaatag aattaatgaa ccgggacagc
    44701 ctgttagcct cttcagtgat gaccaggtta ttaaaatcct gaaccctaat gatatgggca
    44761 tagctgataa tccttcccat aaaatacacc agaccaatat tccaacttat atacgtcaaa
    44821 aaataaaata gttcgattta tttttctccc cctattaaaa tccaaaagcc cctgtttatt
    44881 caggggcttt tagtttgaat ccttagagta ttaatatcag acggcttttt gaattgtacc
    44941 gctctttaga caggtcagac aaacttttac agttttaatt gaaccactat tatccactat
    45001 tctaatttta cgcagattgg gttcccatat ccgatgagtt ctattattag catgactaac
    45061 tttattgccg tgaatcggct tttttccgca aatatcacaa acttttgaca ttttcattac
    45121 tccttaaaaa gc
//
LOCUS       DAOMJU010000006        43052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_707_length_43052_cov_19.751936, whole genome shotgun sequence.
ACCESSION   DAOMJU010000006 DAOMJU010000000
VERSION     DAOMJU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 43052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43052
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_707_length_43052_cov_19.751936"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PLO58_01145"
     CDS             complement(<1..1214)
                     /locus_tag="PLO58_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO73426.1"
                     /translation="MKEKTGVLSLKEKIGYSLGDTASNLFFQTFIFFLLYFYTDVFGI
                     PASSVATIFLIGRIWDAVNDPLMGAIADRTNTKMGKFRPYLLYTALPFGIFGFIMFIT
                     PDLSVQGKVIYAFITYNLMMMTYTVINVPYSALMGVISPHSEERTVVSTFRFVAAFVG
                     QFIVQFAVLRLVSVFGKGNEQIGWQWTMAVMAGLSVVLWLVTFATTKERVKPVKGQKN
                     PLKQDLADLSKNVPWLLIGSATIFQLLFNVIRGGSIMYYFKYFVQDQQLFLFGKPHDY
                     SYQTLTSTFMLTGTLVTIVGSLLTSFFSKRIGKSRSYWGFLGISAICSALFFFLKPQD
                     VVWMFLLQIIASFCMGPVSVLQWAIYTDTADYSEWRTGRRATALIMAVSLFALKLGIA
                     LGGSILAWILDI"
     gene            complement(1278..3755)
                     /locus_tag="PLO58_01150"
     CDS             complement(1278..3755)
                     /locus_tag="PLO58_01150"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HQO73427.1"
                     /translation="MTVHFQGMSKTLQFPQTIALEGAPTCSIGISPEGLYNRLNDAGR
                     LILLNQNAIQFGSAPFIKITADPNAEDERDEYFMLTTRDPNIVEGPDDVPALKNYYHP
                     PVPEAKVTISLATPLAEIIYDLAKISVTRLITSPLFSGNDQTLIPISVEEFVVENTSD
                     KTQEVTLVIPRPSLVNLQEKELKPTDQDTVYICSASVKGQVHEAFNSAGIKGVVMGNQ
                     ECQEKMAIAVPEIEGVDIDTHPHFCLNHLKQDLLLNDDGSFYEKRNVIPHQDYGAAIS
                     LHFTLGPKASLKIPFAIALDFPQQRFIDGVEFERKYVKLFKNQASRTIEMIKVALENY
                     PEWLNRTLTIQNRIYELIRKNPAYRDDEEGALRLARLIFNEFSCPISNACVWVKDNEG
                     KERARFLECFDYAYINPSDVDWYSMVLLFLFPKIEEEICQGFINSILAEDPTPRFYHL
                     HASFVEARQHFEKYPEEYEDISLTQIWDATKVKGSVAHDLGALPKGHALRNVSDYTWY
                     NNNYWIDLFPKLATRVLRNVKFTGNTAFVKENWETLKFGFDHLQELDFDGDGIPEGYP
                     GEVKNTFDNLTLFGVDAYDATIFMAGCYAMIKMAEIVKDTAAKKQYEEIFDKVSKEFE
                     KLWRDTKNSQGKRLQYYVTCFDPKTGETNTDVWMNQLDAIWALIAMGEESFIPEERIK
                     QILKTVYDNNRAYMGWAMCRTEDGGKVESEQGQDVYTTSNYVFAQLLDYYGMTEESKE
                     VYKAMDKVIFGHGNTLISPDNLRAELEQEAGESAPGPHYIVAAYPRPGAILTQIVLQY
                     IKETQKQTKNMLVDSDKLKSFAISLMK"
     gene            complement(3970..5787)
                     /locus_tag="PLO58_01155"
     CDS             complement(3970..5787)
                     /locus_tag="PLO58_01155"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HQO73428.1"
                     /translation="MSKERDVFIQKKIAGMTLMEKIGQLLTFTRRGTLLTPSGIEQIT
                     RLHCGGLCLEPYAVETCKNLYWGNSQIDKNFKPPKDYFTIANNYFDGKTFGISITPED
                     YTKDLNRLQKIAMSRPSGIPLHMTIDFEGDFKNDYMSGGIRQFPGPMGMAAIGDEKLT
                     YEIGKTIARMLTAIGVTQMYSPVCDVNTNPRNPEIGVRSFSDDPEVCARHAVALIKGL
                     QDYGIAATAKHFPGRGDSGSDAHDVLEVLNLDKKRMHEVELVPFKAAINAGVKAIMTG
                     HSVYPAFDDKYPTTLSEKILTGLLREELGFEGVIVSDAIGMAAILKQWPLPQACAMAI
                     KAGCDTILLKADDESRSQCFFGIKMAVERGELSEERIDQSVARLLKMKYDQGLFESAG
                     EKDPEITREITTAKEVIDFSWDVAKKALIVMRDEQHLLPLDPQQRILVIEQRIPYEFL
                     GKDPYCHTHMFCEAMVNHSQNLILADTAFSAYEDEIKECLELAKQADLVVMTNYYARI
                     VKSGNNRLLVKKLKEAGHKVIVVTNNPYIEGTTVDADAVVCNFSGTPDSIRVSTYLLY
                     GKVIPCPTTKLPIKPDVGEKTPVKDLKAPKKHPLNLSYC"
     gene            complement(5805..7022)
                     /locus_tag="PLO58_01160"
     CDS             complement(5805..7022)
                     /locus_tag="PLO58_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQO73429.1"
                     /translation="MEKKKNLEIVAAGHTCLDLIPAFTIEGEVEKMTDVLVPGKMINM
                     GECVIAGGGPVTNAGVSIRRLGVKTELIGKVGNDDFGRLVLDWYEKNEGHFKGIKMVD
                     GESTSYTIAICIPGIDRFYLHHCGANDTFGYDDMDFNLVGRAKLMLFGYPPWMRKIYE
                     NTGAELTKILKKTKELGTTTALDLSLPDVDSYAGQVDWKAILTDWVPLSDIMVPSAEE
                     LFYFLYKDKFLEKRDKLGHKETVLDQMTVKEISTMGRDLIRMGAAIAMIKCGSRGLYI
                     YTAGQERLKKMGAAGCADLENWANRELWFPVYQEEKFVGALGSGDSAIAGFLSAFVWG
                     HSIESCLRYANAAGSMNVTVPDGLSWNKGFDDLTRRIKAGWKTKEMKINEPGWKKERE
                     FWVGPDNRGKWES"
     gene            complement(7119..8225)
                     /locus_tag="PLO58_01165"
     CDS             complement(7119..8225)
                     /locus_tag="PLO58_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HQO73430.1"
                     /translation="MTANSQAENLSHLTLKEAFKDDFKIGAALDLNQISGRDEQGLAI
                     VEKHFNSITPENILKWEEVHPEPDRYNFDAADQFVAYGEKHNMQIIGHCLVWFFQTPD
                     WVFEDGAGKPLSRAALLARMKEHIFTVMGRYKGRIHGWDVVNEALMIDGTLRKCKWLD
                     IIGEDYILKAFEYAHEADPEAELYYNEYDTEHKSKREALIRLIQDLQAKGARVDGIGT
                     QGHWFLDYPSREEIEKAILEISTTNVKQMITELDVGVLPFYPVDSQMVDISSFDPEMQ
                     KKYNPYPDGLPDSVQKELARRYAELFTIFLKHSDKIDRVTFWAVHDKQSWRSYLPIRG
                     RVEYPMLFDRQCRPKPALEAILKVAQNRAKLVEN"
     gene            complement(8963..10375)
                     /locus_tag="PLO58_01170"
     CDS             complement(8963..10375)
                     /locus_tag="PLO58_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQO73431.1"
                     /translation="MTTFKKYVNKIALDKLVTYCIKDPQKHIPKLIDFVDKSAWLPIH
                     KSYVQGAKSIVNNPDSNWYQLAMRAIRDIDPDIIHKIVRNLFINSGFEGQGKLVEERE
                     RLGFGIPWAVLMDPTSACNLKCKGCWASEYDKSLNLDTDLMDRIIREGKELGIYFYIY
                     SGGEPLIRKNDLINLAEKHSDCVFLSFTNGTLVDEHFADELRRVGNFSLALSIEGDEK
                     ATDFRRGKGSYQKLISAMKILKSRGIPFGFSTCYHSQNWNIVGEPEYLDQMIEAGCYF
                     GWYFTYIPLGKHADLSLLARPEHREHMYYRVREARQTKPIFLIDFWNDGEYVNGCIAG
                     GRRYLHINANGDVEPCAFIHYSDTNIKNVSLLEALQSPLFLQYQRNQPFNQNHLRPCP
                     LLDNPDALKSIVLETGAYSTQHMDREDVVELTAKCQDIAQAWKLTADRLWEETLKNRV
                     QKKSGVKQYERELVDAEIHN"
     gene            complement(10397..10750)
                     /locus_tag="PLO58_01175"
     CDS             complement(10397..10750)
                     /locus_tag="PLO58_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQO73432.1"
                     /translation="MKVKKENKSIKKEDPKIIDMSTFDELIHSNKPVVVEFYDEKIST
                     SYIVDSILSRLANYFQNQIEFLRLHINQHRAIADKYHIFSSPATLIFLNGKLIEVILG
                     TFSQNQIIEILNRLL"
     gene            complement(10895..12340)
                     /locus_tag="PLO58_01180"
     CDS             complement(10895..12340)
                     /locus_tag="PLO58_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQO73433.1"
                     /translation="MAKIDPNSDYYSELSQFTIESDVDAILWIDAESTIYRVNQAACQ
                     LLQYSPEELVGKNLDDLIDIQNDWNKTDPHRFLDQIDIIKQNGVTVIERNFRTKDGRI
                     LTLEISAHLFVFKNNEYICVFARDITERRKAQEELQKTLKELEFLKNKLYEENIYLQN
                     EIKLTHNFEEIVGKSLSLRKVLEKVEQVAATDATVLIQGETGTGKELIARTIHHISSR
                     SSSPLVKVNCATLPAALIESELFGHEKGAFTGALYRKMGRFELANGGTIFLDEIGDLP
                     LEIQSKLLRVIQEGEFERIGSNKTLKTDVRIIAATNRNLEAAVQKGEFRKDLYYRLNV
                     FPIHVPPLRERPEDIPLLIHHFVQKYSKKIGRKIDIIPAKIMRTLTSYSWPGNIRELE
                     NIIERAVIITNGRTLQIGNWFTTDAPVMNKEKIQPLREAERDYILKVLSLTNWRVSGP
                     HGAAQLLDINPQTLVSKMKKLGIHRTKPINE"
     gene            complement(12496..14976)
                     /locus_tag="PLO58_01185"
     CDS             complement(12496..14976)
                     /locus_tag="PLO58_01185"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQO73434.1"
                     /translation="MESSKKQSVINEKPTPIKGLTTEEALSRLKKYGPNTFPRSKPPS
                     AWSILISQFKSPLIYIILVAAVISLIMGELTDFIIIIAVVIVDVILGFFQEFQAQRTY
                     TALNKLLKPTTTVIRDGERQEIDVTKVVPGDIVILNAGERIPGDGEIIEGARLSVDEA
                     ILTGESEPIKKSVEAGSNQVFMGTTLLTGRGVMRVTQTGVRTELGKIAKSLQEHKEEE
                     TPLQVRLKSFSRTLTIIVVSFTTIIFLVGLLLKRDFLDMLRTSVILAISAIPEGLLIA
                     VTVILVLGMRKILKRQGLVKKLLAVETLGSVNVICTDKTGTLTEGRMRVTRTDFVDNE
                     RAIQIMVLCNNLEGPVEMALWEYAKSRLPNDPQTLVEESQRLDEEIFTSETKFMITGH
                     HLPDGDYHFLKGAPEIVLKMCQINDDERQRILAQIDQWAGEGLRLLGLAYRDYGILED
                     HTGYTWVGLIGMEDPIREGVHEAVLLAKHAGIQVKMITGDYRRTAERIASNIGLLQNG
                     EETLEGEELAQLNDEELAKRIYPTAVFARIRPHDKLRIVNALQKHGDITAMIGDGVND
                     APALKQANIGVVVGTASDVAKETADLILLDNNFRTIVAAIEEGRVIFENIRKVVAYVL
                     SNSFAEVLTIFISMILGWPAPLLVAQILWIHLICDGPADIVLGFEPKENGIMDEKPKS
                     LKEPILPPLGLFLIGVISIGSALAALLIFGHYFKIHGDADEGRSLVFASLAINSLIYI
                     FAYRSMRKSLFKMNSILKNKPLIFAVIFGMVLVFLPFVIPSLSQALGVVPLDLKQWLL
                     IFTIALMLLIAVEIGKAVSNWWHNKKIL"
     gene            complement(15303..15387)
                     /locus_tag="PLO58_01190"
     tRNA            complement(15303..15387)
                     /locus_tag="PLO58_01190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15351..15353),aa:Leu,seq:taa)
     gene            complement(15422..15494)
                     /locus_tag="PLO58_01195"
     tRNA            complement(15422..15494)
                     /locus_tag="PLO58_01195"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15459..15461),aa:Cys,seq:gca)
     gene            complement(15615..17537)
                     /gene="dnaK"
                     /locus_tag="PLO58_01200"
     CDS             complement(15615..17537)
                     /gene="dnaK"
                     /locus_tag="PLO58_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQO73435.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGKPRVLENSEGRPVTPSVVVFKK
                     DGEIVVGDTAKRQIVTNHDRAVYSIKRFMGRLYKEVQREREEVPFKVVEGPNGDARIR
                     IGDRDYSPPEISAMILQKMKQTAEEKLGEKITDAVITVPAYFNDSQRQATKDAGKIAG
                     LEVKRIINEPTAAALAYGLDKKKEEKIAVYDLGGGTFDISILEIGDGVFEVKSTNGDT
                     HLGGDDFDQRIIEWLISEFKKKEGIDLKKDPMAMQRLKEAAEKAKIELSSNLETEINL
                     PYITAGSAGPIHLMEKLSRAKFESLVDDLIERTRKPCQQALEDAGLKPEDINEVILVG
                     GSTRIPKVQDLVKDLFKKEPNKSVNPDEVVAIGAAIQGGVLAGEVHDILLLDVTPLSL
                     GIETLGGVTTKLIERNTTIPTRKSEIFSTAADNQTSVEINVLQGERAMAADNKSIGRF
                     HLDGIPPAPRGVPQIEVTFDIDANGILNVTAKDKATGKEQSVRIQTSSGLTEEEKEKM
                     VRDAQLHEEEDRRRKEEVDTRNQVDALIYQTEKNMKEYGDKLQPEEKGRLNNALEDLK
                     KAQNSGDVKTMKSAIEAMNQAWSEVSSKMYERVRTETPPPHTGQTYEQPHEETTGQKD
                     ENIQDADFEVVDDDKK"
     gene            complement(17678..20146)
                     /locus_tag="PLO58_01205"
     CDS             complement(17678..20146)
                     /locus_tag="PLO58_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQO73436.1"
                     /translation="MKENFSKIIQIIVKAARYEAGRMNHSYVGSEHLLWAIINMERSV
                     TLEILMALDVDIKALQTMLTDSLKAIGGTATIGQIPFTKQVERILRNTYNEAKELGAE
                     VVGDEHITLSILKEKEGISYEILNSFGVDYYNFRAEISGNMDEPLPVTKSEKKSSRTP
                     ALDHFSRDITEKARTNQLDPVIGRDQEIERVAQILSRRKKNNPVLIGEPGVGKTAIAE
                     GLALRIVAKKVPHLLANKRIIDLDLAAIVAGTKYRGQFEERMKTIMQELEQARDVILF
                     IDELHTIVGAGGASGSLDASNMFKPALARGELQCIGATTLDEYRKFIEKDGALERRFQ
                     KVIINPPSKEESLEILKGLRERYEAHHHVKYTDEALWTAVELSDRYITDKFLPDKAID
                     VMDEAGSRVHLRDVIMPEEILEAEKKLEEVRNQKDNAVREQQFEKAADLRDSERRVKK
                     QLADLQQAWEKSGEKQTLLVTAEDIADVVAMMTGIPVNRIAESENQKLLTMEDELRKS
                     IIGQDDAIRVVSSAIRRARAGLKDPRRPIGSFLFLGPTGVGKTEFARVLARFLFPGTD
                     ALIKIDMSEYMEKFAVSRLIGAPPGYVGYEEGGELTERVRRQPYSLVLFDEIEKAHRD
                     VFNILLQIFDEGVLTDSFGRRVDFKNTIIIMTSNAGSRSLQSSAFGFSKADPLSEYEA
                     MKKRLLEEIQHVFNPEFLNRIDDPIVFKPLTREDVLAIIDLQLEDTRRNLGASGTTLE
                     ITQEAKELILTQGFKPEFGARPLRRAIQNMIEDPISELILAGTLRRGSKIVVSVTDNK
                     IVFTQPEIEFKISSTKITSEPVSN"
     gene            complement(20294..20995)
                     /locus_tag="PLO58_01210"
     CDS             complement(20294..20995)
                     /locus_tag="PLO58_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQO73437.1"
                     /translation="MKNENDKTLLTVKNLSKSYWNDGLEIAVLRNINLTVKEGEVVAI
                     VGPSGIGKSTLLNLLGTLDRPDSGVIEYEGLNPFDLSDTEIARFRNTHIGFVFQFHHL
                     LPEFTALENVMIPAMIYNRDVRKAEEMAREMLARVGLAERATHRPAALSGGERQRVAV
                     ARALINNPLIILADEPTGNLDAMNSELLIELMLELNRIYRRTFIIATHNQTIAEKSHR
                     ILKFTKDSLLIEASI"
     gene            complement(20985..22208)
                     /locus_tag="PLO58_01215"
     CDS             complement(20985..22208)
                     /locus_tag="PLO58_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQO73438.1"
                     /translation="MNKNFLNWIALRYFRSKKKTGLISFTSYISIIGIALGAFAMLVA
                     LSVLNGFEAEITNRVIDIESHLRISGRNLQSAQIERVKEALSGVPVREIYPFVMKKSI
                     LSSEQMEAVVRIKAIESATLEQQLSGPRTMMRGGANFLSATSNLPGIVVGYQLADKLG
                     VYPGDTLNVINPTSLGSGLNIPIIGQFAVTGVFQLDLFDYDENIAFIELKVGQEIFEM
                     GDNYSGIDIRLENYRYVEKAKQQLVAQLGNEYDVASWEDLHRTLFGAMKLEKYGSFLA
                     LSFIILVAILNLTSALVMLIMEKIQEIGMLQALGMNQTQVRQIFLRLGFLIGSIGLTV
                     GIGLALILCLAQQHFQFIPLPSVYYIPYLPVQVQWSDVLMIIGAGLIFIYCGTLYPSW
                     RVGKLMPLEAIQYEK"
     gene            complement(22201..23421)
                     /locus_tag="PLO58_01220"
     CDS             complement(22201..23421)
                     /locus_tag="PLO58_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQO73439.1"
                     /translation="MSVPFFIAKRHLFSRHKIGYISFISIIATISLAVGVAALILTIS
                     ILNGFEQEIKTKLLGFDSHIRLRLLYQDSMDSTLAIRSELEKMPEIECFVPYIHGAAM
                     IRKGQETEGIIVEGIDEVHIRQTLAVDRFIKKGELRFNLPDGRDGIVIGAKLARQLGV
                     DIGDKVYLFVFQPEESYIKRPRIMTFTVTGLYDSGIADYDDVFIYTSLQAAQKLYDLG
                     EKFSGYQIRLKDQSKVEAVTEYINKTLGYPYNALSWNDLHSNLFEWLRIQRIPIMLIF
                     GLIAVVAVFNIVSSIMMIVIEKTRDIGILKSVGMNNRQVKRIFLYEGLFIGVVGTLAG
                     FGLSILLGYLQNRFKLISIPADVYFMSSLPVLLELRNFLIIGLIAIASALLATLYPSY
                     KAVKLTPSEATRYE"
     gene            complement(23434..24933)
                     /gene="lysS"
                     /locus_tag="PLO58_01225"
     CDS             complement(23434..24933)
                     /gene="lysS"
                     /locus_tag="PLO58_01225"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQO73440.1"
                     /translation="MNKEEQATGKSLNELLAIRREKLQAIREMNFRAYEYEFQKTHSA
                     AEITQNFDELTEKVVVRVAGRLMAIRKMGRASFGHIQDESGRIQIYVQENSVGQQGYK
                     LFNLLDIGDIIGITGRVFRTRTGEITILVEELQLLAKNLRPIPIVKEKDGQIFDAFSD
                     KEQRYRQRYLDLMVNPEVREVFKTRSKIIQWTRDFLNEHGFLEVDTPILQPIYGGANA
                     RPFKTYYNALEREFYLRIAVELYLKRLIIGGFEKVYEIGKNFRNEGLDRSHNPEFTLL
                     EFYQTYVDYNYMMDFVEQYFHYLCSKLGESKFPFGEYEIDFSQPFSRRKMFDLIHEYV
                     GEDITQYSEAELKSLCLKKNLELKPGDNYGKYIELLFSNFVEPHLIQPTYVIDYPKAI
                     SPLAKNKRDGSPNLVERFELYIAGHEFVNAFSELNDPIDQRERLEQQNRLRESGDEEA
                     QTMDEDFVTALEYGMPPTGGVGIGIDRVVMLFTNQVSIKDVILFPQMRT"
     gene            complement(24930..26046)
                     /gene="prfB"
                     /locus_tag="PLO58_01230"
     CDS             complement(join(24930..25982,25984..26046))
                     /gene="prfB"
                     /locus_tag="PLO58_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQO73441.1"
                     /translation="MLSDIQVELDNIAPRIDELRRFFDVESKKNELNTERQKTTASDF
                     WSDPAQAQTTLQKIRGLESEIDNFENLVKYQKEITDFVDLAAEEQDDSVLAELEKMVN
                     ELISKVEQLELETMLDEPDDEKNAILTIHPGAGGTESQDWAHMLMRMYLRWMERSGME
                     ADVISILDGEEAGIKEVTIEVKGRYAYGYLKAEAGIHRLVRISPFDANHRRHTSFASV
                     FVYPAIEDDISVEINQDDLRIDTFRASGAGGQHVNKTSSAVRITHIPTGIVVQCQNER
                     SQFKNKDSAMKILRARLYQLKKEEKNKEKMEIEKNKKDISWGNQIRSYVFQPYTLVKD
                     HRTKYETGNIQAVMDGEIDEFLRQYLLFNVKNERAKK"
     gene            complement(26116..26583)
                     /locus_tag="PLO58_01235"
     CDS             complement(26116..26583)
                     /locus_tag="PLO58_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQO73442.1"
                     /translation="MIDSIDVQILDILQKEGRIQRNKIAEQVNLTIPAVSERMRKLEK
                     KGIIEGYHAKINSAAIGKDVTAFVFVMTSPSKNYDEFVRRSREESDIIECHSITGEGS
                     HLLKVQTDNTSALEKLLSKIQSWPGVLQTRTYIVLSSYKDFSSLKPASSLVKK"
     gene            complement(26601..27119)
                     /locus_tag="PLO58_01240"
     CDS             complement(26601..27119)
                     /locus_tag="PLO58_01240"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HQO73443.1"
                     /translation="MKRVSLFMILTLLSVQLYSQESQTITTRSGRTADRLSASKYILS
                     TGSDAMLMTVKIWGEVTRPGLYDVPIGTDLIDLISAAGGPTTRANLSKVKLIHGSPDE
                     EENYVTIVNIKKFLETGDVDMIPEIKPSDTIVVPVKITQHFLTSLSWTQQLLSLFSIY
                     SMILYYTSIANR"
     gene            complement(27135..27890)
                     /locus_tag="PLO58_01245"
     CDS             complement(27135..27890)
                     /locus_tag="PLO58_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73444.1"
                     /translation="MTTSNQINLMVALILLLNTCGKNLEDPDPPAQPEWVAKSLPYDT
                     TETGIDAVPNGDFIQLEWYTGDEDDLSSYRIYRASGDIENKFDLIEEIQSDLMAGSIN
                     IYLDENVSINTPYFYFLRAVDQAGNLSLRSDTVEYKLVVKVNLRGLFDTTDVNPTFKW
                     SDAGSAASEYVIRVEQFNPNRVMCLSATSRQSYTDEVQSLKYGTGIIFYSAQDTLSRG
                     VDYRWRVDAVATFNREGDEVAGSESNWGYFKIQ"
     gene            complement(27896..28906)
                     /locus_tag="PLO58_01250"
     CDS             complement(27896..28906)
                     /locus_tag="PLO58_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73445.1"
                     /translation="MKRILIIGIILISLGSGLVAGEYADAFLELGVSARASAMANAIG
                     ALDFSETAFITNPAGISYIRQARLGLMYTSQFGLADHNYIGLAMPAGVHYSLALSWIR
                     YGVDDIPIRPDIFTTITDKAERRDSIISIYNGKLPTFNDVENAVFVSLGRFNSTIVDL
                     GWRYSKFKLEFPIGINLKFIHKKYYELEGFGLGVDIGGRLRVDGEELLDVSNLGMLSV
                     GMALKDVTGTTIYWNTKSQDRIRLTPALSFALEQPIKPLKLIINLVSEKQYRYHDKTN
                     YGVEIIYANSLALRAGLKNSGISLGLGLNFKVMKKLINLDYSFYSHDLGASHRVGLGI
                     GI"
     gene            complement(28903..>29717)
                     /locus_tag="PLO58_01255"
     CDS             complement(28903..>29717)
                     /locus_tag="PLO58_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HQO73446.1"
                     /translation="TVDLSNCPAASQIDDLNIGKWNPSLRIWYNEEGQKEGMKIKTTS
                     TIPGTFSLITNTDTKPPNLELNLEGQQFFQNSYVSDRPSISIIGEDDNGVRFDAEGIK
                     VIIDNNEVALENLHAPDTVSSGHYFSVQFRPTFTHGNHSMEVTLKDAAGNSTTESVDF
                     IVAEELKLIDYGNYPNPFRNRTTFIYELTQRVNSLKIKIYTTSGRLVRVLDSENVFES
                     GLDLNEGGYHEVVWDGLDADGNFVANGIYFYKISAKSGNKTVTKTGKLAKAR"
     assembly_gap    29718..29817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29818..>32126)
                     /locus_tag="PLO58_01260"
     CDS             complement(<29818..>32126)
                     /locus_tag="PLO58_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HQO73447.1"
                     /translation="KYFRLYKADEDELDFTRPESSIDGLYTFTLSNFSTSDISIYKVG
                     KSKLRDFTTEYIKSTNSYRVMLQDYVRDDTTLYWAACGEALKFPLSVKLDTIIGLSSI
                     PSGAEVVVITRQEWVNNLSKLTDFYASIGLSSQVVGIEDIYNEFNAGIISPFAIKKFL
                     QYVSYNWNPVPEYILLVGDADIKEERSIPSYFFQSYSYGACASDFWYALIDDDDVPDY
                     AIGRWPCSTIEELEALIDKRLNYTKHNLIGPWNNELLFIAGQENVFKNQSENMIQRQI
                     SKEYNINRIYVNPASVGSRFYGGSDTLLYLFNNGITLGNFMGHGGGAVWGDRSLFNSS
                     HIPELENLDRLPFLTSMTCFTGDFTNVTGLGEMLLLAENGGAIGLWGASSVGWIKNDY
                     LLAKPFYDVIFEPGMTVGKAIQTAKIRYQAEAFDYLRLSLIYSYNLIGDPTVSLPFPE
                     QSTLLNLSQTNPAPGDTVILRGDLPFEAGKMYIQLFDSSKYAVYEKPAEVPFSSSNLH
                     HEIILPTNINPGNTYVNYYIESKTAKQDAHGATLFDIKGLTFYGFDYAPSTPGEKTPI
                     ILSIYTELSDVQSFFCEIDTVSAYEYLDKDGIEHIISFQDDSVLLSIRMGEVESVANK
                     WQTTQVFQVGTPGKLVAVRFVAIDSSGNRTVSPAYSIRIKSLPDLRPIGIAQGGERFP
                     ELIAEVSYNGNDTLSAVVKAERRVDEKFELFGSKEVVFLPNRATSVKLPGILGKDTVI
                     FRITVDPENTVAESNEMNNSLSQSMVINT"
     assembly_gap    32127..32226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32227..33434)
                     /locus_tag="PLO58_01265"
     CDS             complement(<32227..33434)
                     /locus_tag="PLO58_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73448.1"
                     /translation="MSIKPVPFYLSHNIKHPLRILFCGFILLISQNLLWSAVSDIEIV
                     EKWLNATTWQWQAELPMPKFPVDEKNQTCAIEISGLENLARDGYPSIPVYQKIINALP
                     DEIAFTVQTGLEKVVPLPAVMSISQDIPIAGEGTNQEKIILPKSKNFSAYPMRLVEIE
                     FLGYLKGIPLSSVKIFPCQLIEQGKRLKYFGSVTVQVQVKPGTRLQKSVATDEQALLK
                     ALDIAPGSIRKIAKQSPTLAKPAVTFPAGKVLAKLIVDEDGIYRVSCRALSDSGIALK
                     KVDPRTFRLFNQQHEVPIYVYGESDGIFHNNDYIEFFGQRNRNSVADYEYDPFTDKNV
                     YWLTWGENYGLRYAEESVQVNSPIDETVVPIDYLYTAHIEVNAAFERLGQVDVDQPTH
                     VRDHWFFDSG"
     gene            complement(33456..34232)
                     /gene="cdaA"
                     /locus_tag="PLO58_01270"
     CDS             complement(33456..34232)
                     /gene="cdaA"
                     /locus_tag="PLO58_01270"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HQO73449.1"
                     /translation="MTLFTIKFLSVTIWDVIDIIVVYIIILKLYQFFKGSRASQMLIG
                     LAIILLTSFVARALNLQSLSWIMGNLQTIWAIAFVIIFQPELRRILIYLGQSRFVRRF
                     LSESKSKTIEAVIEASRELVRRKWGGLFVLVREVGLKPIKERATPINAEVSASLLVSI
                     FNPGSPLHDGAVLIQNDIIEAAGCILPLSDSPDLDPQMGTRHRAALGISEESDAVVVV
                     VSEETQKIAVAYQGMLYRNVDEDSLRALLSRLFFRGSNNS"
     gene            complement(34233..35339)
                     /gene="folP"
                     /locus_tag="PLO58_01275"
     CDS             complement(34233..35339)
                     /gene="folP"
                     /locus_tag="PLO58_01275"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQO73450.1"
                     /translation="MVRKSEFLILKTDPIIAPAANILKQQILSVGGDCAVAREVPTGQ
                     ALPTPVILMATRRQYERLIINLKQQCFGLDKLAVEIEEFLVRISAPRKFHIGQQEYQT
                     DQKTLIMGILNVTPDSFYDGGRHAEMSAAIETALEMEADGADLIDIGGESTRPGSDPI
                     PAEVEIARLKPVLEQLQMRLKIPISIDTYKSSVAEWALNNGALIINDISGLNFDSQMA
                     GTIAKHNAGVILMHIKGTPKNMQADPHYTNLIDEIMKYLSDSVQKARTAGIDGSKIFI
                     DPGIGFGKTAEDNYRIMRYLAEFQSLGLPILVGPSRKAFIGQVLNLPADERLEGTLAA
                     VTAAVLNGADLVRVHDVKAAVRAVKIADAIKGKS"
     gene            complement(35410..37485)
                     /gene="ftsH"
                     /locus_tag="PLO58_01280"
     CDS             complement(35410..37485)
                     /gene="ftsH"
                     /locus_tag="PLO58_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQO73451.1"
                     /translation="MSENNYPKNQSSKNLNEPRKPRQPRGRSPEPPKPNKDDFRWKKA
                     SRTSLMWIIIILSSIVLLQILGRGAQEEVVITYSQYKELLDQKLIKSAVIIENEFHGT
                     LTQKVPLVVGGRTIEAEKILVNLRFVDSEMLKSWDEAGIEYDFKVKNTKWTNFFINIF
                     PLLFIILMTLFFLRRMQGQNNKGIFGFGKSKARLWTSDRPKVSFNDVAGCAEAKEELQ
                     EIIEFLKSPSKFSRLGGKIPKGALLLGPPGTGKTLLAKAVAGEAGVPFFSISGADFVE
                     MFVGVGASRVRDLFEQGKHSAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVE
                     MDGFDENTNVIILAATNRPDVLDTALLRPGRFDRQIVVDMPDLVGREGILKVHTKKIP
                     LADDVDLSVLARGTPGLVGADLANLVNEAALLAARKNKKAVEMIDFEEAKDKVMMGVE
                     RRSLHISDRERRLTAYHESGHVLVAVNIPEADPIHKVTIVPRGRALGITHQLPMDERH
                     NYSRSYINSRLSILLGGRAAEKIIFNELTTGAGNDIETATELARKMVCEWGMSDKLGP
                     LTFGKKNEEIFLGREIATHRDFSEETARLIDQEIREIVENAEKQACEIIKKNIDLLHK
                     LALALLEKEVLDGAEIAKILGNSEVNSANIFVNKSDDNMTKPATKAKTGSRRRKSEKE
                     KGANTDQAA"
     gene            complement(37509..38066)
                     /gene="hpt"
                     /locus_tag="PLO58_01285"
     CDS             complement(37509..38066)
                     /gene="hpt"
                     /locus_tag="PLO58_01285"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HQO73452.1"
                     /translation="MKKTITLDASYGPYAGQVIEELLTVTQINRRVTELAAEISAMYQ
                     GTVPIFIGVLNGSFIFFADLFRKLTIDGEVDFIKISSYADQTRSSGTILLRKDISCDI
                     TGREVIIVEDIIDSGLSIKFLKRRMEESNPCSVRFAALLMKEDARVDFPIDFVGFKIP
                     NKFVVGYGLDVAQRLRNLASVYAFK"
     gene            complement(38063..39457)
                     /gene="tilS"
                     /locus_tag="PLO58_01290"
     CDS             complement(38063..39457)
                     /gene="tilS"
                     /locus_tag="PLO58_01290"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQO73453.1"
                     /translation="MKPTTLQRKFVEYVEQNHLLNDVRTVVAAVSGGVDSMCLLDLLM
                     SEKKRWDIEIVVAHFNHQIRGPEADSDENLVRQFCDNHSLPFFSAAENVPKIARAKRI
                     SLEMAGRELRYAFFQKIAADFPNSIIATAHTLDDQAETILLRMLKGTGLQGLSGITMR
                     RENIRRPLLFATKAELYDYARARELPFAEDHTNLCNDYERNFIRNQILPTVQHTLNPN
                     IRATLANLAQNLGEAHQFIVEFAEKALESLIIKANSEQIVLDKSSLKTYFIAVEKEII
                     WQSLQRLDGTIKPLNFKQMNQLIKLVNEGETGRRMNLGGEITVLIDRERLILKRNRSS
                     NWSSSPVIPGEKIKNEFFTFSSELMDVSNFKKGKRRSGVEFIDFDLVGNNLGLRHWQR
                     GDRMIPLGMNQSKKISDILVDLKVPFTDKNSIPVFYSEDKIIWLCGLTLSDEFKVTSK
                     TKTVLKLIYKGKKI"
     gene            complement(39454..40086)
                     /locus_tag="PLO58_01295"
     CDS             complement(39454..40086)
                     /locus_tag="PLO58_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQO73454.1"
                     /translation="MLIEQIITQQFSENVWLVGDPQTRKGLIIDPGDHADEIIGKISA
                     MNIQPLAILNTHAHPDHLAAAAEVQNHYNIPFYLHAKEKAVLKSAIPFGLVLGIRITR
                     VPSQVIYLQDEETLKIEPFEIRVIATPGHTPGSVCYLIADRLFSGDTLFRNSVGRVDF
                     PGSSWQELKRSLEKLKNLPPTTEVYPGHGMNTTIGQELSHNPYLKNGEWR"
     gene            complement(40248..41318)
                     /locus_tag="PLO58_01300"
     CDS             complement(40248..41318)
                     /locus_tag="PLO58_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73455.1"
                     /translation="MARKIVHIIGTGTVGAPLIGVLLGHLEQLGIEEISFEPNLKAIE
                     NKAFLNGLVTRGAKLCVREEIADEFISVGCKIDYTSKEAIERAGVIIDCSPGDEPLKN
                     KAEIYSRLDGEPKFFIAQSRAAGFGLDYAYGINDEALVSSEVQFVRVVSCNAHNIAAM
                     IKTLAFDEKGENYLDWGRFVCIRRASDISENKPFIPAPQISLPQDDNYGTFQALDTAS
                     LFRTIGVNLDIFSSALKVNSQYLHIVNFDLKLKRPVTLPLVINKLESNPLIALTNKTQ
                     SSLVFAFARDMGFLGRILNQSVVSVPSLTVKGDREIIGFCFSPQDGNSILSSIAATVW
                     YFYPHSYRDKLQNLDDLIFAEV"
     gene            41809..42153
                     /locus_tag="PLO58_01305"
     CDS             41809..42153
                     /locus_tag="PLO58_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73456.1"
                     /translation="MDRKSALKVRESGQDWAEKNICTINANPAKGKVQPVSAGNSKFQ
                     ASNSKEAPQTKYQTLPIPDRCGHWYLKFIWYLVLETWYLIPGIRTFVFANNQKKGVYY
                     VMSVRKAEARSQ"
     gene            42238..42900
                     /locus_tag="PLO58_01310"
     CDS             42238..42900
                     /locus_tag="PLO58_01310"
                     /inference="COORDINATES: protein motif:HMM:NF022745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3103 family protein"
                     /protein_id="HQO73457.1"
                     /translation="MKRKWRTDGESAILVAYTPLTTDDMEWEEIYAYDADLKEYILDA
                     KTEPDYPVIVVGINERIEYNKATIVQTADKIIAKTAEIRQSEEILDSIRIAIDGEGWP
                     NGDPEIYIVAAGTNTTWDRRERVNLASVNKINKWYPIDGALFSWETKYWGTHTGYGVL
                     EYDPDGYNYSVTILSEDVEVEVKKKSDGNDEFIGNISSIPYDHTNGVIWLLDNAKIGL
                     SY"
BASE COUNT        12166 a         9781 c         8294 g        12611 t
ORIGIN      
        1 taaatatcca gaatccaggc taaaatggag ccaccgaggg ctatgccaag tttcagagca
       61 aatagtgaaa ccgccataat aagtgccgta gcgcgacgcc ccgttctcca ttccgaataa
      121 tctgccgtat cggtataaat cgcccattgt agaaccgaaa ccggacccat acaaaatgaa
      181 gcaattattt gcaataaaaa catccagacc acatcctgcg gcttaaggaa aaagaaaaga
      241 gctgagcaga tagccgaaat tccaagaaag ccccaataac ttctcgattt tccaatcctt
      301 ttactgaaaa aactggtcaa taaggaccca acgattgtaa ctaaggtacc agtaagcata
      361 aaagttgagg tcaaagtctg gtaggagtaa tcatgtggtt taccaaataa aaataactgc
      421 tgatcctgga cgaagtactt gaaataatac ataatactgc cgccgcgaat cacgttgaac
      481 agtagttgaa aaatagtcgc tgagccaatc agaagccatg gcacattttt cgaaagatca
      541 gccaaatcct gcttcaacgg gttcttttgc cccttgaccg gtttgacacg ctctttggtc
      601 gtggcgaagg tcactaacca gagaacaacc gacaagccag ccatcaccgc catagtccat
      661 tgccaaccga tttgttcatt tccttttcca aaaacagaca ctaatcttag gacggcaaac
      721 tggacgatga actggcctac aaaggcggcg acaaaacgga aagttgagac aacggtccgt
      781 tcctctgaat ggggtgatat tacacccatt aaagccgaat aaggtacatt gatcactgta
      841 taggtcatca tcatcaaatt ataggtaata aaagcgtaaa tcactttccc ctgaacgctt
      901 aaatccgggg taatgaacat tatgaagcca aaaataccaa agggcaaggc ggtataaagc
      961 aaatacggtc ggaatttacc cattttggta ttagttcgat cagcaatcgc ccccattagt
     1021 ggatcattga ccgcatccca aattcgacca attaaaaaga ttgtcgcgac ggatgaggcg
     1081 ggaataccaa atacatcagt ataaaagtaa agcaaaaaga agataaaagt ctggaaaaaa
     1141 agatttgaag ccgtatcacc taaactgtag ccgatttttt ctttcagtga aagcactccg
     1201 gttttttctt tcataatgca ccctctgttt ttagaatttt aataacgttc gtaatttttc
     1261 tgttttaatc aaagctctta tttcattaag gaaatcgcaa aactcttcaa tttatcagaa
     1321 tccacaagca tatttttcgt ttgcttctgc gtctctttaa tgtactggag cactatttga
     1381 gtcagaattg ccccaggacg cggataagcg gcaacgatat aatgcggacc aggtgctgat
     1441 tcgcctgctt cttgctccag ctctgcgcgc aaattatcag gtgatattag ggtatttcca
     1501 tgtccaaaga tcaccttgtc catcgcctta tatacttcct tactttcctc cgtcatacca
     1561 taataatcta gcagttgtgc aaatacataa ttggaagtag tatagacatc ctgtccctgt
     1621 tccgattcaa cttttccgcc gtcttctgtt cgacacattg cccagcccat ataggcacga
     1681 ttattatcgt agactgtttt aagaatttgc ttaatgcgct cttcgggtat aaaagactct
     1741 tcccccatag caatcaatgc ccagatggca tccaactgat tcatccagac atcagtattg
     1801 gtttctcccg tcttgggatc aaaacaggtg acatagtatt gaagtctttt gccttggctg
     1861 tttttcgtgt ctctccataa tttttcaaat tctttagaaa ctttatcaaa aatctcttca
     1921 tactgctttt ttgccgcggt atcttttaca atctcagcca ttttgatcat tgcataacaa
     1981 cctgccataa aaatggtcgc atcgtaggca tctacaccaa ataacgtcaa attatcaaaa
     2041 gtatttttga cttcgccggg atacccttcc gggataccgt ctccatcgaa atcaagctct
     2101 tgcaaatgat caaaaccgaa tttaagagtt tcccaattct cctttacaaa agcagtattg
     2161 ccggtgaatt tgacattgcg caggacgcgc gttgccagtt tagggaaaag atctatccag
     2221 taattattat tataccaggt gtaatcactg acattgcgga gcgcgtgccc tttcggcaaa
     2281 gcacccagat cgtgcgctac actacctttt actttggtgg cgtcccaaat ctgagttagt
     2341 gaaatgtctt cgtactcttc tgggtatttt tcaaaatgct gacgtgcttc aacaaaagaa
     2401 gcatgcaaat ggtaaaatcg cggggtcgga tcttccgcca ggattgagtt gatgaagcct
     2461 tgacaaattt cttcctcaat cttagggaag agaaatagca acaccatcga ataccagtct
     2521 acgtccgaag gattgatata agcataatca aagcattcga ggaatcgggc tctctctttg
     2581 ccttcattat ctttaaccca aacacaggcg ttcgatatcg ggcaactaaa ttcattaaaa
     2641 atcaggcggg ctaatcgcaa agcaccttct tcgtcatctc tataggctgg attttttcga
     2701 attagctcgt aaatccggtt ttggatggtc aatgttcgat tcagccactc agggtagttt
     2761 tccagagcaa cttttatcat ttctatggtt cgactggctt gattcttgaa taatttgaca
     2821 tacttccgct caaattctac cccatcgata aaacgctgtt ggggaaaatc cagagcaatt
     2881 gcaaagggaa tttttagact ggctttaggt cccagagtaa aatgtaaact aatcgccgcg
     2941 ccataatcct gatgcggaat tacattacgt ttttcataaa aactgccatc gtcgttaagc
     3001 aacagatcct gtttcaaatg attaaggcag aaatgcgggt gagtatcaat atcaacaccc
     3061 tctatttcag gaacagcgat cgccattttt tcctggcact cttgattgcc catgacgaca
     3121 cctttgattc cggctgaatt aaaggcttca tgcacctgcc ctttgactga agccgagcaa
     3181 atatataccg tatcttgatc ggttggcttt agttcttttt cctgaagatt taccagtgag
     3241 ggacgcggta ttactagggt cacttcttga gttttatcgg aagtattctc gaccacaaat
     3301 tcttctacgc taataggaat aagggtctgg tcatttccgg aaaataaagg cgaggtaata
     3361 aggcgagtta cacttatttt cgccaaatca taaataatct ctgccaaggg agtagccagt
     3421 gaaatggtta cttttgcttc gggaactggc ggatgatagt aattttttaa tgccggtaca
     3481 tcatcggggc cttctacgat attaggatct ctggtagtta gcatgaaata ttcgtcgcgt
     3541 tcgtcttcag catttgggtc agccgtgatt ttaataaacg gggctgaacc aaattggatg
     3601 gcattttgat ttagcaaaat taatcgccca gcatcattaa ggcggttata taaaccctcc
     3661 ggagaaatac ctattgaaca agtcggagca ccttccagag caatggtctg cggaaattgc
     3721 agagtcttgg acattccctg aaagtgtact gtcattttgt tatctttgta aatcttagat
     3781 atttgtcttc cggaaatagt gaagacgtcc ctaatcttaa ttttttccat gctattcttc
     3841 ccgatttata attaaaagat acaataatta cctgtctgtg gcattagtaa tttcttattc
     3901 aataccatat aaaaaattcc aataatttgc catacttcat agagtccatc cccttgcaga
     3961 tctcttcatc taacaataag aaagattcag cggatgtttt ttaggtgcct tgagatcctt
     4021 aactggggtt ttctccccga catcaggctt tatgggcaat ttagtagtcg gacagggtat
     4081 gactttgccg tacagcaggt aagtcgaaac tcgaatcgaa tctggcgttc cgctgaaatt
     4141 gcaaacgacg gcgtcggcat ctacggtagt gccttcgatg taaggattat tagtaacgac
     4201 aatgacttta tgtccggctt ccttcaattt cttgactaaa agccgattat taccggattt
     4261 cacaatgcgg gcatagtaat tagtcataac gaccaggtca gcctgtttgg ctaattcgag
     4321 gcactcctta atctcatctt cataagccga aaaggctgta tccgccaaaa tcaaattctg
     4381 actgtggttg accattgctt cgcaaaacat atgcgtatgg caataagggt ctttgccaag
     4441 aaactcatac ggaatgcgtt gctcaatgac taaaattctc tgctgggggt ccaatggcaa
     4501 aaggtgttgc tcatcgcgca ttacgattaa ggctttttta gccacatccc aagagaaatc
     4561 aattacttct ttagcggtgg taatttcccg agttatctca gggtcttttt ctccggcgga
     4621 ttcaaacaat ccctgatcat atttcatttt caggagacgc gcgaccgact gatcaattct
     4681 ctcttcactc agttcgcctc gctcgaccgc catcttaatc ccgaagaaac actgtgagcg
     4741 agactcatca tcggctttta agagaatggt atcacagccg gctttgatcg ccattgcgca
     4801 ggcttgtggt aaaggccatt gctttaaaat tgccgccata ccgatagcat cggatacaat
     4861 cacgccttcg aatcccagct cttcccgcag gagaccagtc aggatttttt ctgacaaggt
     4921 agtaggatat ttgtcgtcaa aggccgggta gacggaatgg ccggtcatta tcgccttgac
     4981 tccggcatta atagccgctt tgaaaggcac taactcaact tcgtgcattc ttttcttgtc
     5041 gagatttagc acttccagca catcatgagc atccgaacct gaatcacctc ggccaggaaa
     5101 atgtttggcg gttgccgcaa taccgtaatc ttgtaatcct tttattaatg cgacggcatg
     5161 tctggcgcaa acttcaggat catcactaaa agagcgcacg ccaatctctg gatttcttgg
     5221 atttgtattg acatcacaga ccggcgagta catctgggtt acgccaattg ctgttaacat
     5281 tctggcaatg gtttttccga tttcataagt caatttctca tcaccgatag ccgccattcc
     5341 catcggaccg ggaaactgac gaataccgcc ggacatataa tcatttttaa aatcaccttc
     5401 aaaatcaatg gtcatatgga gaggaatacc cgaaggccgg ctcatagcaa ttttctgcaa
     5461 gcggtttaag tctttggtat aatcttccgg cgtaatgctg ataccgaagg tttttccatc
     5521 aaaataatta ttagcgatgg taaaataatc ttttggtggt ttgaagttct tatctatctg
     5581 ggaattgccc caatacaggt ttttgcaagt ctcgactgca tacggttcga gacacagacc
     5641 gccgcaatgc aaacgagtaa tctgctcgat tccactcggg gtcagaagcg ttcctctgcg
     5701 cgtgaaagtc agcaactgcc cgattttttc cattaaagtc attccggcta tttttttctg
     5761 aataaataca tctctctctt tcgacatttt attctcctat tattttatga ttcccattta
     5821 ccacgattat ccggaccaac ccagaactcg cgttcttttt tccagcccgg ttcgtttatt
     5881 ttcatctctt tggttttcca tccggctttg atgcgtctgg tcaaatcatc aaatccttta
     5941 ttccagctta aaccgtcagg caccgtaaca ttcatactac cggcggcatt agcatagcgc
     6001 aaacaagatt cgatgctatg cccccagaca aaggctgaga gaaatccggc gatagccgaa
     6061 tcaccggagc ccagcgcgcc aacgaacttt tcttcctgat aaaccggaaa ccagagttcg
     6121 cgattagccc aattctcaag gtcggcacat cccgccgcgc ccatcttctt caagcgttct
     6181 tggccggcag tgtaaatata aaggccgcga ctaccgcact ttatcattgc gatcgccgct
     6241 cccattctta tcaggtccct gcccatcgta gaaatttcct taacggtcat ttggtcgaga
     6301 acggtctcct tatgtccaag tttatccctt ttttccaaga atttatcttt atatagaaaa
     6361 tagaacaatt cctccgcact cggcaccata atatccgata acggtaccca gtcagttaaa
     6421 atagctttcc agtcaacctg tccggcataa ctgtcaacat ctggcagaga aaggtcgaga
     6481 gccgttgtag ttcccaactc tttggttttt ttaaggattt tagttagctc agcgccggtg
     6541 ttttcgtata ttttacgcat ccacggtgga tagccgaaaa gcattaattt ggcacgtccc
     6601 accaaattaa aatccatatc gtcataacca aaggtatcat tagccccgca atgatgcaaa
     6661 taaaaacggt caatgccggg aatgcagatt gcgatggtat aggaagttga ttcgccatca
     6721 accattttaa tacctttaaa atgaccttca tttttctcgt accaatctaa aacaagacgg
     6781 ccgaaatcgt catttccaac ttttccaatt agttcggttt tcactcccag acgccggatt
     6841 gataccccgg cattagtaac cggcccgcca ccggcaatca cacattcacc catattgatc
     6901 attttgccgg ggacaaggac atctgtcatc ttttcaactt ctccctcaat tgtaaaggct
     6961 gggatcaaat caaggcaagt atgcccggcg gcgacaattt caagattttt tttcttttcc
     7021 atttgtaatt cttcctgaaa taattaaacg ttatagcgac ggatcgcatc ctgattacag
     7081 aataaccgat tctccatttc gctcttatct ttcaacaatt aattctccac taatttagct
     7141 cgattctgag caacttttaa aattgcttcc agagccggtt taggtcggca ttgtcggtca
     7201 aacaacatcg gatattccac ccttcctcta atcggcaagt aactacgcca cgattgtttg
     7261 tcgtgaaccg cccaaaatgt cacccggtca attttatcac tatgcttaag aaaaatggta
     7321 aagagttccg cataacgcct tgctaattct ttttgaacag aatcaggtaa tccgtccgga
     7381 taaggattat actttttctg catttccggg tcgaacgaac tgatatccac catctgggaa
     7441 tcaaccggat aaaaaggaag cacaccgaca tccagctccg ttatcatttg tttgacatta
     7501 gttgtggaga tctctaagat cgccttctca atttcctctc gagaaggata atccaaaaac
     7561 caatgaccct gagtaccaat tccatccacg cgggcgcctt ttgcctgcaa atcctgaatt
     7621 agtcgaatga gtgcctcacg tttggattta tgttcggtat cgtattcatt gtagtaaagt
     7681 tcggcctcag gatcagcttc atgggcatat tcaaatgcct tgagaatata gtcttcacca
     7741 ataatatcga gccatttaca ctttcgcaaa gtcccatcta tcataagcgc ttcattgacc
     7801 acatcccagc cgtgaatgcg acctttataa cgccccatta ccgtaaaaat gtgttctttc
     7861 atacgcgcta ataatgctgc acggcttaat ggcttgcccg ccccatcctc aaaaacccaa
     7921 tccggcgtct ggaaaaacca gactaaacag tgaccaatga tttgcatatt atgcttctca
     7981 ccataggcaa cgaattgatc cgccgcatcg aaattatatc ggtccggttc cggatggact
     8041 tcttcccact ttaatatatt ttcaggcgta atgctattaa aatgcttttc gacaatggca
     8101 agcccttgct catcacgacc ggaaatttga ttcaaatcaa gagccgcccc gattttgaaa
     8161 tcatctttaa aagcttcttt taaggttaaa tggctgaggt tttccgcttg gctattagct
     8221 gtcataagtc ctattcctta gaattggata ttcggaaata ttgctgatga ttacgttcag
     8281 aaataaccgg aaatactctc ataatgttat cattaacaga gaggaatttt taactatgca
     8341 atcttttaat aattctaatt tttcgagcat aatgttaacg ttgaatatga aatttgaatt
     8401 attcattgaa ccgtttcaaa tatatttaac actttttaat ttaccaataa aaattttagc
     8461 cgtggttaca tctcaatttc tgattgtatt ttcagttcaa attcttactg gcgtactcaa
     8521 accaaattct cctctactca tcggataaag aggataaaga ataatgtcaa ccttttttct
     8581 aagaagacat tcgccgtaat ttctaaattg ttaaataatc aatatcataa caataggttt
     8641 aataccttta aaaatagata atgaagacgg aagaaaatat taaatccttc atctcattct
     8701 ggtttttctt aattagtcgt tataaaaaaa gcggccggag agataaccga ccgctttttg
     8761 ggaggaagag agactattga gtctcgggag gaaaaaattc ttacttttta tttatgagta
     8821 tatagttgtg aaataacaat caattccgcc aattattaaa attaatctat ttactaaagt
     8881 accaccgatt agttcactga tgaaaagcca tattcccagt atgcctaaat cgcttgccac
     8941 agaatgtctc ttttcattta tatcaattat ggatttctgc atcaacgagt tcccgctcat
     9001 attgtttcac tccggacttc ttttggactc gattttttag agtctcttcc cataatctat
     9061 cggctgtaag tttccaggct tgcgctatat cctgacattt agcagttaat tctaccacat
     9121 cctcacgatc catatgttga gtcgaatatg ccccagtctc cagtacaatt gattttaaag
     9181 cgtcaggatt atccagcaat ggacatggtc tcaggtgatt ttgattaaac ggttgattcc
     9241 tctgatattg gagaaacaat ggagattgta aagcctctaa taaagatacg ttcttaatgt
     9301 tggtgtccga ataatgaata aaggcacaag gttcgacatc gccattggcg ttaatatgca
     9361 aataacgacg cccaccggca atacaaccgt tgacatattc cccgtcattc cagaaatcta
     9421 tcaagaaaat tggtttagtc tggcgcgcct cacgcacacg gtagtacata tgttcccgat
     9481 gttccggacg ggcaagcaaa ctaagatcag cgtgtttccc aagtggaata taagtaaagt
     9541 accagccaaa ataacatccg gcttcaatca tctgatcaag atattccggt tcccccacga
     9601 tattccagtt ctggctatga taacaggtcg aaaagccaaa aggaattcct ctcgatttca
     9661 aaattttcat tgcagaaatc agcttctggt aactgccctt gcctcttcgg aaatcggttg
     9721 ctttttcatc accttcaatg cttaatgcca gcgaaaaatt tcctactctt cgtaattcat
     9781 ccgcaaagtg ttcgtccact aatgtgccat tcgtaaaaga tagaaagacg caatccgagt
     9841 gcttttcagc tagattaatc aaatcgtttt tacgtatcag cggttcacca ccactgtaga
     9901 tatagaagta aataccaagt tctttacctt cccgaattat tcgatccatt agatcagtat
     9961 caagattgag actcttgtca tactcactag cccagcaacc tttacacttc aagttacaag
    10021 cactggttgg atccataagc accgcccagg gaataccaaa accgagcctc tccctctctt
    10081 caactaactt accctgtccc tcgaagccag aattaataaa cagatttctc actattttat
    10141 ggataatatc cggatctata tccctaattg ctcgcatagc tagttgatac cagttggaat
    10201 cgggattatt gacaatgctt tttgctcctt gtacatagct tttatgaatt ggaagccagg
    10261 cacttttatc aacaaaatca atgagtttag gaatatgctt ctgcgggtct ttgatacagt
    10321 aggttactaa tttgtcgagt gcaattttat tgacgtattt cttaaaagtc gtcatattat
    10381 taacctcctt tttttactaa agaagacggt taagaatctc gattatctgg ttctgcgaaa
    10441 aagtcccgag gataacctcg attagtttac cgtttaaaaa gattaaagtg gctggtgatg
    10501 aaaaaatatg atatttatcg gcaattgctc gatgttgatt tatatgaagc ctcaaaaatt
    10561 caatctggtt ctgaaaatag ttggcgagcc gggacaatat cgaatcaact atataactcg
    10621 tgctaatttt ttcatcgtaa aattcgacca ccaccggttt gttgctatga atcaactcat
    10681 caaaagttga catatcgatg attttcggat cttctttttt aatactctta ttttcttttt
    10741 ttaccttcat acaattttat taaggacaaa atctatacca aatccttgac tttgtttgat
    10801 aacagtctat taattaaaga ataatgtaat aattcgatca aaaattgctg ggtttagcaa
    10861 ttgattattt tatgatatat catataattt attattattc attaattggt ttagtgcgat
    10921 gaatgcccaa tttcttcatt ttggagacta acgtttgggg attgatatcc aggagttgag
    10981 ccgcaccgtg aggcccgcta actcgccagt ttgtcaaaga tagtactttc aagatgtaat
    11041 ccctttcggc ttctcgcaag ggttgaattt tctccttatt catcactggc gcatcagttg
    11101 taaaccaatt accaatctga agagttcgcc cgttggtaat gataacggct ctttcgataa
    11161 tattttctag ttcccggata tttcctggcc aactatagct tgttaaagta cgcattattt
    11221 tagctggtat aatatctatt ttgcgaccga ttttcttcga atatttttgg acaaaatgat
    11281 gtattaagag aggaatatct tctggccttt cgcgcagagg cggcacatga atgggaaaaa
    11341 cattcaaccg ataataaagg tctttacgaa actcaccttt ttggacagcg gcttcaagat
    11401 tacgattagt ggctgctatt atacgtacgt cggttttgag ggtcttgtta cttccaatcc
    11461 gttcaaattc accttcctga attactcgta aaagtttaga ttgaatttcc agtggtaaat
    11521 caccaatttc atccagaaaa attgtaccgc cgttcgccag ttcaaaacgt cccattttac
    11581 gataaagtgc cccggtaaaa gctcctttct catgtccgaa taattcgctt tcgattaagg
    11641 cggctggtaa agtagcacaa tttaccttga ctaatggact ggaactacga gaactaatat
    11701 gatgaatagt gcgggcaata agttctttcc cggttcctgt ctcgccttgt attaaaacgg
    11761 tagcgtcggt tgcggctacc tgttccactt tttccagaac ttttctgaga ctcagacttt
    11821 tgccgacaat ttcttcaaag ttgtgagtta gtttaatttc attctgaagg taaatatttt
    11881 cttcatataa tttatttttt aaaaattcca attccttcag tgtcttttgc agctcttcct
    11941 gggctttcct ccgttcagtt atatcccggg caaacacgca gatatattca ttatttttga
    12001 aaacaaataa atgcgctgag atttccaaag tcaaaatccg accgtctttc gtacggaaat
    12061 tgcgctcgat aactgtgaca ccattttgtt ttatgatatc aatctggtct aaaaagcggt
    12121 gtgggtcggt tttattccaa tcattctgaa tgtcaattag atcatctaaa tttttaccga
    12181 ccaattcttc tggcgaatat tgcagtaatt gacaagcggc ttgattgacc cggtaaatcg
    12241 tactctcagc atcgatccaa agaatagcat ccacatcact ctcgatcgta aactgggaga
    12301 gttcagaata gtaatcggag tttggatcaa ttttggccat agttgctttc aacaatcaat
    12361 tgactaattc caaaataaac aaagtaattt aaaatatcaa attaagtaag caaatataaa
    12421 caatattgct gagattagac ccaaaaacga gttttagttg cattttctat taggataaac
    12481 atttttatcc tttagtcaga gtattttttt attgtgccac cagttgctga ctgccttgcc
    12541 aatttccacc gcaataagaa gcattaatgc tatcgtgaag attaataacc attgtttcag
    12601 gtccaggggt acgacgccca aagcttggct taaactgggt attacaaaag gcagaaaaac
    12661 cagcaccatt ccaaaaatta cggcaaagat taacggcttg tttttcagga tcgagttcat
    12721 tttaaaaagt gacttgcgca tacttcggta agcgaaaata taaattagtg aatttatcgc
    12781 caggctagcg aagactaaac tacggccttc gtcagcatca ccgtgtattt taaaatagtg
    12841 gccaaaaatc aggagagccg ctagcgcact gccgatacta atcacaccaa ttaaaaacaa
    12901 gcctaaagga ggcaaaattg gttctttaag agattttggc ttttcgtcca taatgccatt
    12961 ctccttcggc tcaaaaccga gcacgatatc agccggacca tcacagatca agtgaatcca
    13021 gagtatctgc gcaaccagta atggtgccgg ccagcccaaa atcatagata taaaaatagt
    13081 caatacctcg gcgaaagaat tggagaggac ataggctacg actttgcgga tgttctcaaa
    13141 gattacgcgc ccttcctcaa ttgcggcaac gatggtgcga aaattgttat cgagaagaat
    13201 caaatcggcg gtctctttag ccacatcgct ggctgtgccg acaaccacgc cgatattagc
    13261 ctgtttaagt gccggtgcgt cattgacgcc atccccgatc atagccgtaa tatcgccgtg
    13321 tttctgcaaa gcgttaacta tccgtaattt atcatgaggc ctaatccggg caaaaacggc
    13381 ggtagggtat atacgtttag caagttcctc gtcatttaat tgagcaagtt cttcgccttc
    13441 cagtgtttcc tcaccgtttt gcaatagacc gatattactg gcaatacgtt cggcagtgcg
    13501 gcgataatcg ccggttatca ttttaacctg aatacctgca tgtttagcga gcagaaccgc
    13561 ctcgtgcact ccctcgcgaa tcgggtcttc cataccgatc aagcctaccc aggtataacc
    13621 ggtgtgatct tccaggattc catagtcacg gtaagccaga ccgagaagac gcaatccttc
    13681 accagcccat tgatcaattt gagccaaaat tcgctgacgt tcatcatcgt tgatttgaca
    13741 catcttaagg acaatttcgg gcgcaccctt caggaaatga taatcgccat caggtaagtg
    13801 gtgaccggta atcataaatt tggtttcact ggtgaatatt tcttcatcca gacgttggct
    13861 ctcttcaact aaagtctggg gatcattcgg cagtcgagat ttggcatact cccacaaagc
    13921 catttcaacc ggtccctcaa ggttattaca cagaaccata atctggatgg ctcgctcgtt
    13981 atccacaaaa tcggtacgag tcacacgcat tcggccttcg gtaagagtac ccgtcttatc
    14041 ggtacagatg acattgacag aacctaaagt ttcaacggct aacagtttct taactaagcc
    14101 ttggcgctta aggatcttac gcatccccaa taccaaaata acagtaacag caatcaacaa
    14161 gccctccggt attgccgaaa tagccaaaat aaccgaagtc cgtagcatat ccagaaaatc
    14221 acgcttcagt aaaagtccga cgagaaatat aatagtagtg aacgaaacga cgataattgt
    14281 caacgtcctg ctgaaagatt taagtcgcac ttgtaacgga gtctcttctt ctttatgttc
    14341 ctgaaggctt ttggcaattt tgcctaactc ggttcgaaca ccggtttggg tgactcgcat
    14401 tactcctcta ccagtaagga gtgttgtacc catgaaaacc tggttagagc cggcttcgac
    14461 tgacttttta atcggctcac tctcgccggt caggattgcc tcgtctacac tgagcctggc
    14521 gccctcaata atttcaccgt caccgggaat acgctctcct gcatttagta taacgatatc
    14581 acccggtact actttcgtta catcaatctc ctggcgttct ccatcgcgga taacagtagt
    14641 ggtcggtttt agcaatttat tcaatgccgt ataagtacgt tgagcctgaa attcctggaa
    14701 aaatccgaga atgacatcaa caataacgac ggctataatt ataatgaaat cagtcagttc
    14761 gcccataatg agtgaaatca cagcggctac caggattata taaataaggg ggcttttaaa
    14821 ttgagaaatt aggattgacc aagctgaggg tggtttactt cttgggaacg tattaggacc
    14881 atattttttt agccgagaaa gcgcttcctc tgtcgttagt cctttgatag gagtgggttt
    14941 ttcgttgatt accgattgtt ttttagatga ttccattttt taactccttc tgagctgtgg
    15001 ttatgatcca gcagtaaaca taaaaaaagg cctgcgagat cgtgttagcg gtcccacagg
    15061 cctatacctg ctgcaatatt gcccagctct atcaacctaa tagatagttg atcgcttgat
    15121 cctcattctc ttaaatttca aataatatgt attttacttt actttatgtg taaaatcaat
    15181 aattaaattc aaattcaaat tcaggatgat taatcgtaga tacaaatggt agtgtttgag
    15241 atatcattct attctaactt ttaaaataaa aggagactct tagtctctta ttatgaaagt
    15301 tgtgccgaag gccggaatcg aaccggcacg atccaaagga tcgagggatt ttaagtccct
    15361 tgcgtctacc atttccgcca cttcggcaaa atgaactata tgaaaaaact gctcaaatca
    15421 gaggcgacga ccggacttga accggtgcat aacggttttg cagaccgctc ccttagccac
    15481 ttgggtacgt cgccaattaa aaaaagcggt gaaaattaga ttttattact caaaaaaaca
    15541 aaaccttttc cagtttcaag ggctgataat aaaaaagggc ggaaagccgc cctttttacc
    15601 atttacgaat tcatttactt cttatcgtcg tccactactt caaaatcagc atcttgaatg
    15661 ttttcatctt tttgaccggt cgtttcctca tgtggttgtt cataagtctg accagtatgt
    15721 ggtggtggtg tttccgtgcg tactctttcg tacatcttag atgatacttc tgaccaggct
    15781 tgattcatgg cttcgatcgc actcttcatc gtttttacat caccactatt ctgggctttt
    15841 ttcaagtctt ccagtgcgtt gttcagtctg cctttttctt ccggttgcag tttatcgccg
    15901 tattctttca tattcttttc tgtctggtaa atcaaggcat caacctgatt gcgagtatcg
    15961 acttcttctt tgcgacggcg atcttcttct tcatgcagtt gagcatcgcg taccattttt
    16021 tctttctctt cctcggttaa accactggaa gtttgaattc ggacgctttg ttctttgccg
    16081 gtagctttat ctttagcggt aacgttcaga ataccattgg catcaatgtc aaaagtgacc
    16141 tcaatttgag gaacaccacg tggtgctggt ggaataccat cgagatggaa gcgcccaatg
    16201 ctcttgttat cggccgccat tgcccgctca ccctggagaa cgttgatttc tacgctggtt
    16261 tgattatctg cggcagtcga gaaaatttca ctctttcggg tcggaatggt cgtattacgc
    16321 tcaatcaatt tagtcgtaac tccacccagt gtttcaattc ccagtgacaa gggcgttacg
    16381 tccagcagta gaatatcgtg aacttctcct gctaaaacac caccctgaat tgccgctccg
    16441 attgcaacta cttcatccgg attgacgctc ttattaggtt ccttcttaaa aaggtctttg
    16501 actagatcct gcactttggg tatgcgagtg gaaccgccta ctaggattac ctcatttata
    16561 tcttccggtt ttaatccggc atcttccaat gcttgctgac acggcttacg agtgcgttca
    16621 atcaaatcat caacgagaga ttcaaatttc gctcgtgaca atttttccat tagatggata
    16681 ggaccagcag aaccagctgt aatatagggt aaattgattt ccgtttccag atttgaagaa
    16741 agttcaattt tagctttctc agcggcttct ttaagtctct gcattgccat cggatctttt
    16801 ttaagatcaa tcccttcttt ctttttaaat tcactaatca accactcaat aatacgctga
    16861 tcgaaatcat cgccacccaa gtgggtgtcg ccattagtgg attttacttc aaaaacacca
    16921 tctccaattt ctaggatgga aatatcaaat gtaccgccac ccaaatcata aacggcaatt
    16981 ttttcttcct ttttcttatc aagaccataa gccaaagcgg ctgccgtcgg ttcattgatg
    17041 atacgtttta cttccaaacc ggcaatttta ccggcatctt tggtagcttg ccgctgagaa
    17101 tcattgaagt aagccggtac ggtaataacc gcatcggtaa ttttttcgcc taatttttct
    17161 tcagcggttt gcttcatttt ctgcagaatc attgcactga tttctggtgg tgaataatcc
    17221 cgatcgccaa ttcgtattcg agcatctcca ttagggcctt caactacttt aaacggaact
    17281 tcttcacgtt ctcgttgaac ttctttatac aaacgtccca taaaacgttt aatcgaataa
    17341 acggcgcgat cgtggttggt tacgatttga cgtttggcag tatcaccaac tactatttcg
    17401 ccatcttttt taaaaactac cacggatggc gtcaccggac gtccttcgga attttcaaga
    17461 acacgtggct taccaccttc catcacagcc acacaagaat tggttgttcc taaatcaata
    17521 cctataattt ttcccatatt tatctccttt ttttgggctt caatttttca aatttcacta
    17581 aaagattgac aaacctcgtg ccaaatagtt attaggacac aacgtcagac tatcttacta
    17641 aatactatat ttggtttgaa aagactgaaa ttatgactta attgctgacc ggttcgctcg
    17701 taattttcgt ggaagaaatt ttaaattcta tttcaggttg agtaaagacg atcttgttat
    17761 cagtaacact aaccacgatt ttactcccgc gtcttaaagt gccagccaga atcaattctg
    17821 aaatcggatc ctcgatcata ttttgaattg cccgacgtag tggacgcgct ccaaattcgg
    17881 gcttaaatcc ctgcgtaaga attagttctt tggcttcctg agtgatttct aaggtcgtcc
    17941 ctgaagctcc tagattgcgg cgagtatcct ccaattgcaa atcaataatc gctaaaacgt
    18001 cttcacgagt taagggttta aagacgatcg gatcatcaat ccggtttaag aattccggat
    18061 tgaatacatg ttgaatttct tccagcaagc gttttttcat agcctcatat tcgctaagcg
    18121 gatcggcttt ggaaaaacca aacgccgagc tctggagact gcgcgaacca gcgttggaag
    18181 tcataattat gatcgtattt ttaaaatcaa cccgacgacc aaaactatca gtcaaaacgc
    18241 cttcatcgaa aatctgcagt aaaatattaa aaacgtcgcg gtgggctttt tcaatctcat
    18301 cgaaaagcac cagtgaataa ggttgacgcc gaacccgttc agtcaattca ccaccctctt
    18361 cataaccgac atatcccgga ggcgccccaa ttagtcgcga aactgcaaat ttttccatat
    18421 actccgacat atcaatttta atcagagcat ctgtgccggg aaataagaag cgagccagta
    18481 ctctggcaaa ttcggtcttt ccaacgcctg ttggacctaa aaataagaaa gatccgattg
    18541 ggcgccgcgg atctttcaag ccggcacggg cacggcgaat cgcactactt acgactcgaa
    18601 ttgcatcgtc ctggccgata attgacttgc ggagttcatc ttccatcgtc agtaattttt
    18661 gattttccga ttcggcaatt ctgttaaccg gaatcccggt catcatcgct actacatcag
    18721 caatatcctc ggcagtaact aataaggttt gtttttcgcc acttttttcc caggcttgct
    18781 gtaaatcggc taattgtttt ttaacccgcc gttcgctgtc ccgcaggtca gcggcttttt
    18841 caaactgctg ttctctaacg gcgttatctt tctgattgcg gacttcttcc agtttctttt
    18901 cagcctcaag tatttcctcc ggcataataa catctcgtag atgaacccgc gagccggctt
    18961 cgtccataac atcaatggct ttatcaggta aaaatttatc ggtaatataa cggtcactga
    19021 gttcgaccgc cgtccataaa gcttcatcgg tatatttgac gtgatgatgg gcttcatagc
    19081 gctcccgtaa tccttttagg atttccagcg attcttcttt acttggagga ttaattatga
    19141 ctttttggaa acgtctttct aacgcgccat ctttttcaat aaatttgcga tattcgtcga
    19201 gcgtagtcgc accaatacac tgtaactcac cgcgcgctaa agccggtttg aacatattag
    19261 aggcatccag cgaaccggac gctccccctg ctccaactat ggtatgcagt tcatctataa
    19321 agagaatcac atcgcgcgcc tgctcgagtt cctgcataat ggttttcata cgttcttcga
    19381 attgaccacg atatttggta ccggcgacga tcgcggccaa atccaaatca atgatccgct
    19441 tgttcgcaag tagatgcgga acttttttag ccacaattcg cagagctaat ccctccgcaa
    19501 ttgcagtttt gccaacacca ggctcgccga ttagcactgg attgtttttc ttgcgacgtg
    19561 acaaaatctg agctactcgt tcaatttctt gatcgcgacc aatcactgga tcaagttggt
    19621 tagtccgggc tttttccgtt atatcccgag agaagtggtc aagtgccggt gttcgtgaac
    19681 tctttttctc actttttgta acgggaagtg gctcatccat atttcctgaa atttcagccc
    19741 gaaaattata ataatcaacg ccaaaactat tgagaatttc gtaggaaatt ccctcctttt
    19801 ctttcaaaat tgatagagta atatgttcat caccaacgac ttcggcgccc agttccttgg
    19861 cttcgttata agtattacga agaatccgct ccacctgttt agtaaacgga atctgtccga
    19921 ttgtcgccgt tccaccgatc gcttttaatg aatcagtcag catagtttgt aaggctttga
    19981 tatcaacatc gagcgccatc aatatctcaa gagtaacgct tcgctccata ttgattatcg
    20041 cccagagcaa gtgttcggag ccaacataac tatgattcat ccggcctgct tcatagcggg
    20101 cggctttgac aataatttga attatcttgg aaaaattctc tttcattatt tcaattcttt
    20161 ctcaaataac atcagaagtt aaaactctct gactgctaag tcaatctttt ctttcctttt
    20221 ttatgttatg caaaatcttt gccactttgg cgtagcattt ttcagtaatt aaccacctaa
    20281 cttatttggg tagttaaatg ctggcttcga taagcagaga atccttggtg aatttcaaaa
    20341 tgcggtggga tttttcggca attgtctgat tatgagtagc aatgataaaa gtccgccgat
    20401 atatccgatt caattctaac attaattcaa tcaatagctc actgttcatc gcatcgagat
    20461 taccagtcgg ttcatctgct aaaataatca aaggattatt gattagcgcc cgggcgacgg
    20521 ctactcgctg gcgttcacca cctgacaatg ccgccggccg atgggtagcc cgttcagcga
    20581 gtccgactcg tgcaagcatt tcacgtgcca tttcttccgc ttttcggaca tctcggttat
    20641 aaatcatagc cggaatcata acattttcga gagctgtaaa ttccggcaaa aggtgatgaa
    20701 attgaaaaac aaatccaatg tgagtattgc ggaagcgcgc aatttccgta tctgataaat
    20761 caaacggatt aagcccctca tattctatga cgccggaatc cggtcggtcg agagtcccta
    20821 atagattcag cagagttgat ttaccaatgc cggatggccc aacgattgcc acaacttcac
    20881 cttccttaac cgttaaatta atattgcgga gcacggcaat ttccaagccg tcgttccagt
    20941 atgatttcga tagattcttt acggtcaata aggttttgtc attctcattt ttcatattga
    21001 atagcctcca aaggcataag tttaccaact cgccagctgg gataaagcgt tccgcaatag
    21061 atgaagatca aaccggcgcc aatgatcatc aaaacgtcgc tccattgcac ctgcaccggc
    21121 agataaggaa tataatatac cgacggaagc ggtataaatt gaaaatgctg ttgcgccaga
    21181 caaaggatta atgccagccc tattccaacc gtcaaaccga tgctcccgat taaaaatccc
    21241 agtctaagaa aaatctggcg aacttgagtc tggttcatac ccagtgcttg gagcataccg
    21301 atttcctgga ttttttccat aatcagcatc actaatgcac tggtcaagtt caggatcgcc
    21361 accagaataa tgaaactaag cgccagaaaa ctgccgtatt tttctaattt catcgctcca
    21421 aacaaggtac gatgcaaatc ttcccaagaa gccacatcat attcgttacc gagctgagca
    21481 accaattgtt gcttggcttt ctcgacgtag cgataatttt ccaaacgaat gtcaattccg
    21541 ctgtaattat ctcccatttc gaatatttcc tggccgactt ttaattcgat aaaagctata
    21601 ttctcatcgt aatcgaataa gtccagttga aaaactccag taacggcaaa ttgaccgata
    21661 attggaatat tcaagccgct tcccaggctg gttggattaa tcacatttaa agtatcgccc
    21721 ggatagacac cgagtttatc ggctaattga taaccaacca caatgccggg tagattcgaa
    21781 gttgccgata agaaattcgc cccgccgcgc atcatagtcc gcggaccgga aagttgttgt
    21841 tccagcgtag cagactcgat ggctttaatc cgcaccacgg cttccatttg ttccgaagac
    21901 aatatactct ttttcattac aaatggataa atctcgcgca ctggcacgcc ggatagcgct
    21961 tctttaaccc gttcaatttg agctgattgg agattgcgtc cgctaatccg cagatgtgat
    22021 tcgatgtcga tgactctatt ggtgatttcc gcctcaaaac cgtttaacac actcaaagcc
    22081 accagcatcg caaacgctcc cagtgcgatg ccgataatgg agatataaga cgtaaaagaa
    22141 atcaaaccgg tcttcttttt agaccggaaa taacgtaaag cgatccagtt cagaaaattc
    22201 ttattcataa cgggtcgcct cactgggagt caatttaacg gctttatacg acggataaag
    22261 cgtggctaat agcgccgaag ctatcgcaat taacccgatt attaaaaaat tccgcaactc
    22321 taacaatacc ggcagggaac tcataaaata aacatccgcc gggatggaaa ttaatttaaa
    22381 ccggttctgg agataaccta ataatatact cagcccaaat ccagccaaag ttcctacaac
    22441 tccgataaac agcccttcat ataaaaatat gcgcttcacc tgacgattat tcattcccac
    22501 actcttaagg atgccgatgt cgcgagtttt ttcgatcaca atcatcatta ttgagctgac
    22561 gatattgaaa acggccacga ccgcaatcag accgaaaatc agcattatcg gaatgcgctg
    22621 aatcctcagc cattcgaaaa gattggagtg tagatcattc caactaagcg cattataggg
    22681 atagccaaga gttttgttaa tatattcggt cacggcttcg actttggact gatcttttag
    22741 ccggatttga taaccagaaa atttctcgcc caaatcatag agcttctgcg ccgcttgcaa
    22801 actggtatag ataaaaacgt catcgtaatc agcaatgccc gagtcataca gaccggttac
    22861 cgtaaaagtc ataatgcgcg gtcgtttgat gtagctctct tccggttgaa atacgaagag
    22921 gtaaacctta tcaccaatat ctacgccgag ttgcctggct aatttcgcac caattacaat
    22981 accatcgcga ccatccggca aattaaaacg cagttcgcct tttttaataa agcggtcaac
    23041 cgccagagtt tgtcgaatgt gaacttcgtc aattccctcg acgattattc cttcggtttc
    23101 ctgccctttg cggatcatcg cggctccgtg aatatagggc acgaaacact cgatctccgg
    23161 cattttttcc agctctgacc gaatcgccag cgtcgagtcc attgaatcct gataaagtaa
    23221 acgcaaacgg atatgggaat cgaacccgag taatttggtc ttgatttcct gttcgaaacc
    23281 gttcaggatc gagatcgtca aaattaaagc cgccactccg accgccaagc taatggtagc
    23341 gataatactt atgaaagata tatagccgat tttatgacgg ctgaaaagat ggcgcttggc
    23401 gatgaaaaag ggtacgctca tcgctttact ttctcaggta cgcatctgag gaaaaagaat
    23461 aacgtcctta atcgaaacct gattagtaaa tagcattacg actcggtcaa taccgatccc
    23521 aactccaccg gtcggcggca ttccatattc cagcgccgta acaaaatctt catccatcgt
    23581 ctgggcttct tcgtcgccgg attccctcag ccgattctgc tgttccagac gttcacgctg
    23641 atcaatggga tcattcagtt cactgaaagc attgacaaat tcgtgaccgg cgatataaag
    23701 ttcaaaacgt tcgacgagat ttggtgaacc gtcgcgtttg tttttagcca gtggtgaaat
    23761 ggctttcgga tagtcaatta cataagtcgg ctggatcaaa tgcggttcaa cgaaattaga
    23821 gaacagtaat tcaatatatt taccgtaatt gtcgcctggt ttcaactcga ggtttttctt
    23881 caggcataaa ctcttcaatt cagcttctga atattgggta atatcttcgc caacatattc
    23941 gtgaattaaa tcgaacattt ttcggcggct aaacggttga ctgaagtcaa tttcgtattc
    24001 accgaaagga aatttagatt cccccagctt cgaacaaaga taatggaaat actgttcgac
    24061 aaaatccatc atataattat aatcaacata agtttgataa aattcaagca aggtaaattc
    24121 gggattatga ctgcggtcaa gcccctcgtt ccggaaattt ttaccgattt catagacttt
    24181 ttcaaaaccg ccaatgatta agcgcttaag atagagttca accgctattc gcaagtaaaa
    24241 ttcgcgctcg agagcattat aataagtctt gaaggggcgg gcattagcac cgccatatat
    24301 cggctgaaga attggtgtat cgacttccag aaaaccgtgt tcattgagaa aatctcgcgt
    24361 ccattgaata attttggaac gagttttaaa aacctcccgc acttccggat ttaccatcaa
    24421 atcgagatag cgctgacggt agcgttgttc tttgtcgcta aaagcatcga aaatttgtcc
    24481 gtctttttcc ttgacaatcg gaattgggcg gaggttttta gccaacaatt gcaactcttc
    24541 aactagaatt gtaatctcgc ccgtgcgagt ccgaaagact ctaccggtaa tcccgataat
    24601 atcgccgatg tcaagtaaat tgaatagctt ataaccttgc tgaccgacgc tgttttcctg
    24661 aacataaatt tggattcgtc cactttcatc ctggatatgg ccgaaactgg cgcgtcccat
    24721 tttacggatt gccataagcc gtccggcaac tcttactact actttttcag ttaattcatc
    24781 aaaattctgg gttatttcag ccgccgaatg cgttttttga aattcatatt cgtacgcccg
    24841 gaaattcatt tcacggattg cctgcagttt ttcgcgacga atcgcaagta gttcgtttaa
    24901 tgactttccg gttgcttgtt cttctttatt cactttttag ctctttcatt tttgacattg
    24961 aataataaat actgacgcag gaattcatct atctcgccgt ccatcacggc ttgaatattg
    25021 ccggtttcgt acttcgtgcg atgatccttg actaaagtgt atggctgaaa gacgtaggaa
    25081 cgaatctgat taccccagga aatatctttt ttattcttct cgatctccat cttttcttta
    25141 tttttttctt ccttttttaa ttgatagagg cgcgctctta gaattttcat tgcgctatct
    25201 ttatttttaa actgagagcg ctcattttga cactggacaa cgataccggt cggaatatga
    25261 gtaatccgaa ccgcagaact ggttttattg acatgttgac cgccggcacc gctggcgcgg
    25321 aaggtgtcaa tccgcaggtc atcctgattg atttcgaccg atatatcgtc ttcgattgcc
    25381 ggatagacaa aaaccgaagc gaaggaagta tggcgacgat ggttggcgtc gaatggtgaa
    25441 atgcgcacca ggcgatgaat tccggcttcg gctttgagat agccataggc gtatctacct
    25501 ttgacttcga tagtaacttc cttgatccca gcttcttcac catcgagaat actaatgacg
    25561 tccgcctcca tcccactgcg ttccatccag cgcaaataca tacgcatcag catatgcgcc
    25621 caatcctgtg actcggttcc gccggctccc ggatggatcg tcaaaatagc atttttttca
    25681 tcgtccggtt catcgagcat tgtttccaat tctaattgtt cgactttact aataagttcg
    25741 tttaccatct tttccaattc agccaagaca gagtcatctt gttcctcagc ggctaaatcc
    25801 acgaagtctg tgatttcttt ttgatatttc accagatttt caaaattatc gatttcgctt
    25861 tccagccccc gaattttttg aagagttgtt tgcgcttggg ctggatcaga ccaaaaatcc
    25921 gaggcggtcg ttttctgacg ctcggtattc aattcatttt tcttcgactc gacgtcaaag
    25981 aaacctccgc aattcatcaa tgcggggcgc aatattatct aattcaactt ggatatcact
    26041 caacatttta atattcgacc ttaattaagt atttttaacc ttaataataa aaacggcaaa
    26101 ttgcacgagg aaaatttact tcttaactaa cgagcttgcc ggcttcagcg acgaaaagtc
    26161 cttataactc gaaagtacga tataggtgcg agtttgtaac acacccggcc aggactgaat
    26221 ttttgagagc aatttttcga gggctgaggt attatcagtc tgtactttca ggagatgcga
    26281 gccttcaccg gtgatactat gacattctat tatatcactt tcttcacgac tccgacgtac
    26341 aaattcatca taattcttcg atggcgaagt cattacaaaa acaaaagccg tcacatcctt
    26401 tccgatggcc gcgctattaa ttttagcgtg atagccttcg ataatgcctt ttttttctaa
    26461 tttccgcatc ctttcgctaa cagcgggaat ggtcaggttg acctgctccg caattttatt
    26521 gcgctgaata cggccttcct tctgcaaaat atctaaaatt tggacgtcaa tactgtcaat
    26581 catgtatcac tcctatggtt ttacctattt gctatacttg tgtaataaag tatcatacta
    26641 taaatcgaga acaaacttaa taattgttgc gtccacgata atgacgtcag aaaatgttgc
    26701 gtaatcttga ccggaaccac aatcgtatcg gacggtttga tttcaggaat catatccaca
    26761 tcaccggttt ccagaaattt tttgatatta acaatagtta cgtaattctc ttcctcgtcc
    26821 ggtgaaccgt ggattaattt gactttggat aaatttgccc gggtagttgg accgccagcg
    26881 gctgaaatta aatcaatcaa atcggtgccg attggtacgt cgtaaaggcc tggtctggtt
    26941 acttcgcccc aaattttgac agtcattaac atagcgtctg aaccagttga cagaatatac
    27001 ttggatgcgg acaaccgatc ggctgttcga ccggatctag tagtaatcgt ctgactttcc
    27061 tgagaataaa gttggacgct taataaggtt aaaatcataa ataaagaaac tcttttcaca
    27121 attactccta attattattg aattttaaaa tatccccaat tggattccga accggcgacc
    27181 tcatcgcctt cacgattgaa agtcgccact gcgtcaacgc gccagcggta atcaactcct
    27241 cgacttagcg tatcttgagc tgaatagaaa ataatcccgg ttccatattt taaactctga
    27301 acttcatcgg tataactttg tcgagatgta gctgacaaac acatcacccg attcggatta
    27361 aactgttcta ctcggatgac atattcagaa gcggccgacc cagcatccga ccatttgaaa
    27421 gttgggttca catcagtagt atcaaatagt ccacgtaaat ttactttaac cactagctta
    27481 tactctaccg tatccgaacg cagactaaga tttccagcct ggtctacggc gcgcagaaaa
    27541 taaaaatatg gggtattaat agaaacattt tcgtcgagat agatattgat actccctgcc
    27601 attaagtcag attggatttc ttctatcaga tcaaatttat tttcaatatc tccgctagcc
    27661 cgataaatcc ggtaactgct aaggtcgtcc tcatcgccgg tataccattc taattggata
    27721 aaatcaccat taggaacggc atcaatgccg gtctcggtcg tatcatacgg taaggatttt
    27781 gctacccatt ccggttgagc tggcggatcc ggatcttcca gattttttcc acaggtatta
    27841 agaagtaaaa ttaaagccac cattaaatta atttggttcg acgtcgtcat ttttactaaa
    27901 taccaattcc aagcccgaca cggtggctcg ctccgagatc atgactataa aatgaataat
    27961 ctaagttaat tagtttcttc ataactttaa aatttaaacc taatccgaga cttatgccag
    28021 aattttttaa gccagctcgt agcgccagac tattagcgta aataatttca acgccgtaat
    28081 tggttttatc atggtaacga tattgttttt cagaaactag atttataatc aattttaatg
    28141 gtttaatcgg ctgttccaag gcaaatgaca acgccggggt caaccgaata cgatcctgac
    28201 tcttagtatt ccagtaaatg gttgtaccag tgacgtcttt caaagccatt ccaacactca
    28261 acatccccaa attacttaca tccagcaact cctcgccatc aacccgcaac cgtccgccaa
    28321 tatcaactcc cagtccgaaa ccttcgagtt cgtaatattt tttatggatg aattttaaat
    28381 ttatcccgat tggaaattcc agcttgaatt tcgaatagcg ccaacctaaa tcaactatag
    28441 tcgaattaaa tcgccccagc gagacaaaca ccgcattttc cacatcattg aaagttggca
    28501 atttgccgtt ataaatacta ataatcgaat cacgccgttc agctttatcg gtaattgtag
    28561 taaaaatatc aggccgaatc ggaatatcgt ccactccata gcgaatccag ctcaaagcca
    28621 gtgaataatg gacaccagcc ggcattgcta aaccgatata gttgtgatca gccaatccaa
    28681 attgagaagt gtacatcagc cccagccgcg cctgccggat gtaactgatc cctgccggat
    28741 tagttataaa agccgtttcc gaaaaatcca gcgctccgat ggcattagcc atagccgaag
    28801 ctcgcgcact tactcccaat tcgagaaagg catcggcata ttcgccagca actagtccac
    28861 tacccaggct gatcagtatt atcccaatta tcagaattcg tttcatcgcg ctttggctaa
    28921 tttcccggtt ttagttacgg ttttgttacc ggatttggcg gaaattttgt agaaatagat
    28981 tccattggca acgaaatttc cgtcggcatc gagtccgtcc cacacaactt catgataacc
    29041 accttcgttt aaatccagac ccgattcaaa tacattctcc gaatccagaa cccgcaccag
    29101 acgaccggaa gtggtataga tttttatctt caaggaattt actctctgcg tcagttcata
    29161 aatgaaagtc gtgcgattcc taaagggatt ggggtaattg ccataatcaa tcaattttaa
    29221 ttcctcagca acgatgaaat ccaccgattc ggttgtgcta ttaccagccg cgtccttcaa
    29281 ggttacctcc atcgaatgat taccatgagt aaacgtcgga cggaattgca ccgaaaaata
    29341 atgcccgctc gaaaccgtat ccggtgcgtg caaattctca agggcaactt cattattatc
    29401 aattatcact tttatacctt cggcgtcaaa gcgtacgcca ttatcatctt cgccgataat
    29461 cgaaatactc ggtcggtcgg aaacgtacga attctggaaa aattgctgac cctccagatt
    29521 taattccaga ttaggcggtt tcgtgtcagt attggtaatt agactaaatg tacccggaat
    29581 agtcgaggtt gttttgattt tcataccttc cttttgtcct tcttcattat accaaatacg
    29641 caaggaagga ttccatttgc caatgttcaa atcgtcaatt tgactcgccg ccgggcaatt
    29701 actcaaatca acggtcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa
    29821 gtattaatca ccatagattg tgacagacta ttattcattt cattggattc ggcgactgta
    29881 ttttccgggt caactgtaat tctaaaaatg actgtatctt tgcccaatat acctggtaat
    29941 tttaccgagg tcgcccgatt cggtaaaaat accacttcct ttgaaccaaa caactcaaat
    30001 ttctcatcaa ctctgcgctc cgccttgacg acagcactaa gagtgtcatt accattatat
    30061 gagacctcag caatcaattc cggaaaacgc tcgccgcctt gggcaatccc gatcggacgt
    30121 aaatccggta aactttttat cctaattgaa taagctgggc tgactgtccg attaccactg
    30181 gagtcaattg caacaaagcg gacggctact aatttccccg gcgtgcctac ttggaaaacc
    30241 tgggtggttt gccatttatt agcgaccgat tcaacctcac ccatccgaat tgataataaa
    30301 actgaatcgt cctgaaaact gatgatatgt tcaatgccat ctttgtccaa atattcatag
    30361 gcgctgaccg tatcaatctc gcaaaaaaac gactggacat ccgagagctc ggtataaata
    30421 gacaaaatta taggtgtttt ttcccctggt gtagatggag cataatcaaa accataaaag
    30481 gtcaagcctt taatatcaaa cagcgtcgct ccgtgagcat cttgtttggc agtcttgctt
    30541 tcaatataat aattaacata ggtattaccg ggattaatat tagtgggcaa aattatttca
    30601 tggtgtaaat tactgctact aaacggcacc tcagccggtt tctcatatac cgcatatttg
    30661 ctggaatcga aaagttgaat atacattttc cccgcttcaa aaggcaagtc gccacgcaga
    30721 ataaccgtat cgcccggtgc cggattggtc tggctaaggt taagcagggt agattgttcc
    30781 ggaaaaggca gactaaccgt cggatcaccg attaaattat aagaatatat caatgaaagt
    30841 cttaaataat caaaagcctc agcctgatag cgaattttcg cggtctggat cgccttgcct
    30901 accgtcattc cgggttcaaa gatgacatcg tagaaaggtt tcgccaatag ataatcattt
    30961 ttaatccagc caacactgga tgctccccac aaaccgattg ctccgccatt ttcagccaaa
    31021 aggagcattt cacctaatcc agtcacattg gtgaaatcac cggtaaaaca ggtcatactg
    31081 gtcagaaacg gcagacgatc cagattctcg agttccggaa tatgagacga attgaaaagc
    31141 gagcgatcac cccatacggc accgccgcca tgtcccataa aattacctaa agtaatcccg
    31201 ttattgaata aatataggag cgtatccgaa ccgccgtaga accgcgaacc gaccgaagcc
    31261 ggattgacat aaatacgatt gatattgtat tctttgctaa tctgacgctg gatcatattt
    31321 tctgattgat ttttgaaaac gttttcctgt ccagcaataa atagcagttc attattccag
    31381 ggaccaatga gattgtgttt ggtataattt aggcgcttgt caattagtgc ttccaattct
    31441 tcaatggttg aacatggcca gcgaccaata gcgtaatcag gcacatcatc gtcatctatt
    31501 agagcatacc agaaatcgct ggcacaggct ccgtagctat aagattgaaa aaaataagaa
    31561 gggatggagc gctcttcctt tatatcggca tcgccgacca gcaaaatata ttccggaacg
    31621 ggattccagt tataactaac atactgcagg aattttttga tcgcaaaggg actgataatt
    31681 ccagcattaa actcgttata tatgtcttca attccaacaa cctgagatga caagccgatg
    31741 ctggcataaa aatcagtcaa tttggaaaga ttattgaccc attcctgacg ggtaataacg
    31801 accacttcag caccggaagg gatcgaactt agaccgatta tcgtatccaa ttttaccgaa
    31861 agcggaaatt tcagcgcctc cccgcaagcc gcccaataga gggtcgtatc atctcgcaca
    31921 taatcctgaa gcattacccg gtaggagtta gtgcttttga tgtattccgt cgtaaaatcg
    31981 cggagtttgc tctttcctac tttataaatt gaaatatccg aagtgctgaa attggaaaga
    32041 gtaaaagtat aaagtccgtc aatcgagctt tccggtcggg taaaatcgag ttcgtcctcg
    32101 tcggctttgt atagccggaa gtatttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32221 nnnnnnatcc ccgaatcgaa aaaccagtgg tcgcggacat gagtcggctg atcaacatcc
    32281 acttgcccaa ggcgctcgaa agccgcattg acttcgatat gggcagtata taaatagtca
    32341 attggtacaa ccgtctcatc aatcggagaa ttcacctgaa ccgattcttc ggcgtatcgc
    32401 agaccgtaat tttcacccca ggtcaaccaa taaacatttt tatcggtaaa tggatcgtat
    32461 tcataatcag ccacactgtt gcgattgcgc tggccaaaaa attcgatata gtcattattg
    32521 tggaagattc cgtcagattc gccataaacg taaattggta cttcatgttg ctgattaaat
    32581 aggcggaaag tacgcgggtc aacttttttc aaagcaatgc cgctgtcgct tagcgcccga
    32641 caagaaacgc gataaatccc atcctcatca acaatcaatt tagctaaaac tttaccagcc
    32701 ggaaaagtca ctgccggttt cgctaaagtc ggcgactgtt tagcaatttt tctgattgaa
    32761 ccgggtgcaa tatcgagggc ttttagaagt gcttgttcgt cagttgcaac acttttttgg
    32821 agccgggtcc ctggtttgac ttgcacctga accgtcactg aaccaaaata ttttaagcgc
    32881 ttgccttgct caatcaattg acagggaaaa attttgacac tgctcagtgg gatgcccttc
    32941 aaataaccga gaaattcaat ttctactaaa cgcatcggat aagcgctaaa attttttgac
    33001 ttggggagaa taatcttttc ctgattcgtt ccttctccgg caataggaat atcctgcgat
    33061 atactcatca ctgccggtaa cggcacaact ttttccaaac cagtttgaac tgtaaaagca
    33121 atctcatccg gtaaagcatt aataattttt tgatagacag ggattgacgg atagccatca
    33181 cgcgctaaat tttccaaacc ggaaatttca atggcacagg tttgattttt ctcatctact
    33241 ggaaattttg gcattggtaa ttcggcttgc cattgccagg tcgtagcgtt cagccatttt
    33301 tccacaattt caatatcaga aaccgccgac cacaataaat tttggctgat tagtaaaata
    33361 aatccgcaga ataaaatccg aagtgggtgt ttaatattat gagatagata aaatgggaca
    33421 ggttttatcg acataattac ttacctgttg cccatctagc tattattact gcctctgaag
    33481 aacagccggc ttaacaatgc tcttaaggaa tcctcgtcaa cattacgata taacataccc
    33541 tgataggcaa cggcaatttt ttgggtttcc tccgatacaa ctactaccac cgcatccgat
    33601 tcttcactaa tacccagcgc cgcgcggtgg cgagtaccca tttgcggatc aaggtcaggg
    33661 ctatccgaaa gcgggagaat acaaccggcc gcctcaataa tgtcattctg aattaaaacc
    33721 gccccatcgt gtaatggtga tccgggattg aagattgaaa ctaaaaggct ggctgagact
    33781 tcagcgttga tgggagttgc ccgctctttt atcggcttca aacccacttc ccgaaccagc
    33841 acaaatagac caccccattt acgccgcacc aattcccggg atgcttcgat caccgcctca
    33901 atcgttttgg acttcgattc acttaaaaat cgtctgacaa agcgactctg ccccaagtaa
    33961 attaaaatcc ggcgcagttc cggctgaaaa ataatcacaa aggcaatcgc ccagatggtt
    34021 tgcagattac ccattatcca agatagactc tgcaaattca gcgctctggc aacaaaagag
    34081 gtcagtaaaa ttatcgccag gccgattagc atttgcgaag cacgtgagcc tttgaaaaat
    34141 tgatagagtt taaggattat tatataaacg acgattatat ctattacgtc ccagatggta
    34201 acgcttaaaa atttgatggt aaaaagagtc atttatgatt ttccctttat agcatcggca
    34261 atttttacag cgcgaacggc ggcttttaca tcgtgaacac gcactaaatc agcgccattt
    34321 agaactgcgg ctgtcacagc cgccagagtt ccctccaatc gctcatcagc cggtaaattg
    34381 agtacctgtc cgataaaggc tttccgcgat ggcccgacca gaatgggaag tccgagtgat
    34441 tggaattctg ccaaataacg cattatccgg taattatcct cggctgtttt cccgaaacca
    34501 atccccggat ctataaatat tttcgagccg tcaataccgg cagtgcgagc cttctgaacc
    34561 gaatccgata agtatttcat tatttcatca atcaaattag tataatgcgg atcagcttgc
    34621 atatttttgg gagttccttt gatgtgcatc aggataaccc cagcgttgtg cttggcgatt
    34681 gtacccgcca tctgggaatc aaaattaagc ccgctaatgt cattgattat cagcgcgcca
    34741 ttattcaaag cccactcagc cacggacgat ttataagtat caatcgaaat cggaatttta
    34801 aggcgcatct gcaactgctc caaaaccggc tttagtcggg caatctcaac ctcggctgga
    34861 atcggatcgg aacccggacg ggttgattct cctccaatat caatcagatc ggcgccgtcg
    34921 gcttccatct ccagagcggt ctctatagcg gcggacattt ccgcatgccg accgccgtca
    34981 taaaacgaat ctggcgttac atttaaaata cccataatta aggttttctg gtcggtctgg
    35041 tattcttgct gaccgatatg aaatttcctt ggcgcggaaa ttcgaaccaa aaattcttca
    35101 atttcaaccg cgagtttatc aagtccgaag cattgctgtt tgagattgat tatcaagcgt
    35161 tcatattgac gacgagttgc cattaaaatt accggcgttg gtagtgcttg tccggtgggc
    35221 acttcacgcg ctacggcaca atcgccgccg acagacaata tttgttgttt tagaatattg
    35281 gcggctgggg cgatgatcgg gtcggttttt aggataagga attcgctttt tctaaccata
    35341 atagcaattc ccgccggatc gacatccagt gattccattt cagtgacgag gtgattgagc
    35401 gcgtcgtttt taagccgcct gatcagtatt ggctcctttt tctttttcgc tttttcttcg
    35461 tcgtgaaccg gtctttgctt tagtggctgg cttagtcata ttatcatcac ttttatttac
    35521 gaatatatta gcagaattaa cttcggaatt acccaggatt ttagcaattt cagcgccatc
    35581 taaaacttct ttttcgagta aagctaaggc taatttgtgt agtaaatcaa tattcttttt
    35641 gatgatttca caagcttgct tctcagcatt ttcgactatt tcacgaattt cttgatcaat
    35701 cagacgagcg gtttcttcgc tgaaatcgcg atgagtagca atttcccgac cgaggaagat
    35761 ttcttcattt ttcttaccga aagtaagcgg accgagtttg tcgctcattc cccattcaca
    35821 aaccattttg cgtgccagtt ccgtcgcggt ttcaatatca tttccggctc cagttgtcaa
    35881 ttcattgaag ataattttct ctgcggcgcg tccgccgagc agaatactga gtcggctatt
    35941 gatatatgag cgtgaataat tatgccgctc gtccattggc aattggtgag tgattcccag
    36001 cgcccgacca cgcggcacaa tggttacttt gtggatcggg tcggcttccg gaatattgac
    36061 ggcaactaaa acatgcccgg attcatgata cgcggtcagc cggcgctccc gatccgaaat
    36121 atgtagactt ctcctttcga cgcccatcat tactttgtct ttagcttctt cgaagtcaat
    36181 catctcaacg gcttttttat ttttacgggc ggctaaaagc gcggcttcat taacgagatt
    36241 ggcaagatca gcgcccacca gaccgggcgt tcccctcgcc aaaaccgaca aatcaacatc
    36301 atccgccagg ggaattttct tagtatgcac tttcaaaatc ccttcgcgtc caactaagtc
    36361 gggcatatcc acgacaatct gccgatcgaa gcgccccggc cgcaacaagg cggtatccaa
    36421 aacatcaggc cggttagtcg ccgctaaaat aatgacattg gtattctcat cgaaaccatc
    36481 catctcgacg agcaattggt tcaatgtctg ttcgcgttca tcgtgaccgc cacctaatcc
    36541 ggcaccacgt tgtcgtccaa cggcatcaat ttcatcaata aaaattatac atggcgcact
    36601 atgtttaccc tgttcgaaaa ggtcgcgcac tcgggaggcg ccgacgccca caaacatttc
    36661 cacaaaatca gctccactaa tactgaaaaa cggaactccg gcttcaccgg ctaccgcttt
    36721 agccagaaga gtttttcccg tcccgggagg accgagtaat aacgcacctt tgggaatttt
    36781 gccgcccaga cgcgagaatt tcgacggcga tttcaggaat tcaattattt cctgcaattc
    36841 ctccttggct tcggcacatc ccgccacatc attgaaactg actttaggcc ggtcgctggt
    36901 ccagaggcga gctttgcttt tcccaaaacc gaaaatgccc ttgttatttt gcccttgcat
    36961 tcgacgcaga aaaaatagag tcatcagaat aatgaaaagt aaagggaaaa tattgatgaa
    37021 aaaatttgtc cacttggtat tctttacctt aaaatcatat tcgatcccgg cctcatccca
    37081 gctttttagc atctcactat caacaaaacg taaattgact aaaatttttt cggcttcaat
    37141 cgtcctgccg ccaaccacta agggaacttt ttgagttaac gtcccatgaa attcattctc
    37201 gataatcact gccgatttga tcaatttttg atccagcaat tccttgtatt gcgaataggt
    37261 aatgaccact tcttcttggg cgcctcgtcc gagaatttgt aatagaacga tcgaactgag
    37321 aattatgata atccacatca gggaagtgcg tgaagccttt ttccagcgga aatcgtcttt
    37381 atttggttta ggcggttctg gagatcttcc tcttggctga cggggcttcc tcggttcatt
    37441 taaatttttt gaactttggt ttttagggta gttattttca gacatatagt taaaattcct
    37501 tttttttctt acttaaaagc ataaactgac gctaagttgc gtagtctctg ggcaacgtcc
    37561 agaccatatc cgacaacaaa tttatttggt atcttaaatc ctacaaaatc aatcgggaaa
    37621 tccacccgcg cgtcttcttt catcaacaaa gccgcgaagc gaaccgagca cggattactt
    37681 tcttccatac gacgttttag aaatttgatt gataatccgg aatcaattat atcctcgaca
    37741 attattacct ctcgaccggt tatatcacag ctaatatcct tccgcagtaa aatcgttccg
    37801 gaggatcgag tctgatcggc ataggaagaa attttgataa aatctacttc accgtcaatt
    37861 gttaatttcc ggaatagatc ggcaaaaaag atgaaactgc cgtttaatac cccgataaaa
    37921 atcggtacgg ttccttggta catcgcagag atttcagcag ctagttctgt aactcgccgg
    37981 ttgatttggg tgacggtcag aagttcttcg attacctggc cggcatacgg gccataactg
    38041 gcatccagag ttatagtctt tttcatatct tttttccctt gtagatcaat ttcagaacgg
    38101 ttttagtttt cgaggtaact ttaaattcat ccgacagtgt taagccgcag agccagatga
    38161 ttttatcttc actataaaag actggaatgc tgtttttatc agtgaaggga actttcaaat
    38221 ctaccaaaat atcgctgatt ttcttggatt ggttcatccc gagtggtatc atgcgatcgc
    38281 ctctctgcca atgtcgcaaa ccaagattat ttccgaccag atcaaagtca ataaactcta
    38341 ctcctgagcg ccgtttcccc ttcttgaaat tacttacatc catcagttca ctggaaaagg
    38401 tgaaaaactc attcttaatt ttttcaccgg ggataacggg tgaagatgac caattcgacg
    38461 accgattgcg ctttaaaatc agccgctctc gatcgattag aactgtaatt tcaccgccaa
    38521 gattcatacg ccgtccggtt tcaccttcat tcactaattt aatcaattgg ttcatctgct
    38581 taaaatttaa tggcttaatt gtgccatcta acctttgtaa agattgccag attatctctt
    38641 tttcaactgc aataaaatag gttttaaggc tagatttatc aagcactatt tgttcggaat
    38701 tcgccttaat aattaaactc tccagcgcct tttcggcaaa ttcgacaata aattgatgcg
    38761 cttcgcctaa attttgcgcc aaattagcta aagtcgctct aatattagga tttagagtat
    38821 gttgtactgt cggcagaatc tgattacgaa taaaattacg ttcgtagtcg ttacataaat
    38881 tagtgtggtc ttcggcaaat ggcaattctc tcgcccgggc atagtcatac aattccgctt
    38941 tggtagcaaa caatagcggc cgtctaatat tctcgcgccg catcgtaatt ccagataagc
    39001 cttgcaaacc ggtgcctttt agcattctta gcagaatcgt ctcagcctga tcatcgagag
    39061 tatgcgccgt ggcgatgatt gagttcggaa aatcagccgc aattttctga aaaaaagcat
    39121 agcgcaattc ccgtccagcc atttcgagag agatccgttt agcgcgagcg attttcggca
    39181 cattttccgc tgcggaaaag aatggcaatg agtgattatc gcaaaattgc cggacaaggt
    39241 tttcgtcaga atccgcctca ggaccgcgaa tctgatgatt aaaatgagca acgacaatct
    39301 cgatgtccca gcgctttttc tctgacatta acaaatccag caggcacata gaatccactc
    39361 cacccgaaac ggcggcgact acggttcgca catcatttaa aagatggttt tgctcgacgt
    39421 attctacgaa cttccgctga agagtggtag gtttcatcgc cattcgccat tttttagata
    39481 cggattatga gataattcct gcccaatagt agtgttcata ccgtgaccgg gatagacttc
    39541 ggttgtcggc ggcaaattct ttaatttctc cagactgcgc ttcaattcct gccaggaact
    39601 gcccgggaaa tcaacgcgac cgaccgaatt ccggaacaac gtatcaccgg aaaatagccg
    39661 atcggcgatc agataacaaa cactgccggg agtatgaccg ggcgttgcaa tcactctaat
    39721 ctcaaaaggt tcgattttaa gtgtttcttc atcttgtaga taaatcacct gactggggac
    39781 tcgagtaatt cttattccca gcaccaatcc aaaaggaatg gcggatttca aaacggcttt
    39841 ttctttagca tgaagataaa acggtatatt gtaatgattt tgcacttcag ccgcggcggc
    39901 taaatgatca ggatgcgcat gcgtgttcaa aattgccagc ggttgaatgt tcatcgcgct
    39961 aattttaccg ataatttcat cggcgtggtc accaggatca ataatcaatc ctttccgcgt
    40021 ttgcgggtca cctaccagcc agacattttc tgaaaattgt tgagtaatga tttgttctat
    40081 cagcatattg agtttattcg aaatttaaaa aacgaaaccc gagtcatata atcatacgta
    40141 aagcaaaata cttaaggtgt ataatttgac cttatttaat tcaaaactat tattacgttt
    40201 gttcaataat tactcggcgt tcttagcatc ctctgcggtt aacaaattca gacttcggca
    40261 aaaattagat catccagatt ttgcaatttg tcccggtaag aatgcggata aaagtaccag
    40321 acagtagccg caatcgaact gagaatagaa ttgccatctt ggggcgaaaa gcagaaacct
    40381 attatttccc gatccccttt aacggtaaga ctcggcacgg aaaccaccga ttgattgaga
    40441 atccgaccga ggaatcccat atcacgcgcg aatgcgaata ccaggctgga ctgagtctta
    40501 ttggtcagtg cgatgagcgg attactttca agcttgttga tcactaaagg caaagtaacc
    40561 ggtcgtttca gctttagatc gaaattgaca atatgcagat attgagaatt gactttcagt
    40621 gccgatgaaa aaatatcgag attaactcct atggtgcgaa acagagaagc cgtatccaaa
    40681 gcttgaaaag ttccatagtt gtcatcctgg ggcaggctaa tttgcggtgc aggaataaaa
    40741 ggtttattct cactaatatc actcgcccgg cgaatacaca caaagcgccc ccaatccaag
    40801 taattttcgc ctttttcatc gaaagccaaa gttttaatca tagcggcaat attatgcgca
    40861 ttgcaggaaa caacccggac aaactgaact tcgctcgata ccaaagcctc atcgttgatg
    40921 ccgtaggcat aatccaaacc aaaaccggcc gctctggact gagcgatgaa aaattttggc
    40981 tcaccatcga ggcgcgaata aatttcggct ttatttttca atggctcatc accaggcgaa
    41041 caatctataa tcactcccgc ccgttcaatt gcctctttcg atgtgtaatc aattttgcat
    41101 ccgacgctta taaattcatc ggcaatttcc tcgcgaacgc ataattttgc gccgcgagtc
    41161 accaatccat tcaggaacgc tttattttct atggctttta aatttggttc aaaggagatt
    41221 tcttcgattc ccaattgttc aaggtgaccg agtaacacgc cgattaacgg ggcgccaact
    41281 gttcccgtac caattatatg cacgattttg cgcgccattt attttttact cctgttaaat
    41341 tatcgtcatt ttaaatccaa aatagcctta taaattaaca caaaattttg cgataaaaaa
    41401 ttgttaaaag taaaattata ttagccgtag gacaggcatt cttgcctgtc tattcctatc
    41461 tattatcata cggtgcaggc atgtccgcct gtctattcct gtctatttta tttacgaatt
    41521 atattgccag gccgacagcc tggaaaggct atcgtatgat tagctgactt aagcaatgtc
    41581 aatataaatt aattgaaaaa taaaacagat agtttcaaga tcctaaaata gttcttgact
    41641 aaatcaaaaa tactatataa aatttaaccg ttatttctaa ttgggcttgc gaagaagcgt
    41701 tattctcccc gtttagcggt tcttcaactg agtgtagttg gttttttctc agagtttttg
    41761 gtttgttcgg caatttaatg aatgtaaatt tctatcaaga gggatagaat ggatcgaaaa
    41821 tcagccctga aagtaaggga atccggccag gattgggccg agaaaaatat ctgtactatc
    41881 aacgcaaatc ctgcgaaagg aaaagtacaa cctgtttctg caggaaattc caaatttcaa
    41941 gcatcaaatt ccaaagaagc accacaaacc aaatatcaaa ctctcccaat cccggatcgt
    42001 tgcggacatt ggtatttgaa atttatttgg tatttggtgc ttgaaacctg gtatttaatt
    42061 cccggcattc ggacctttgt attcgctaat aatcagaaga aaggagtata ctatgtcatg
    42121 tccgtcagga aagcagaagc ccggagtcaa tgattgcgca ttttctccgg gccgaacaat
    42181 cgtaatctca gcagagatta cactaattcc gcacgatttt aataagattt ttttgaaatt
    42241 aaaagaaaat ggagaacaga tggagaatcg gctattctgg ttgcctatac tcctctaact
    42301 actgatgata tggaatggga agagatttat gcctatgatg ccgatctgaa agaatatatt
    42361 ctggatgcca aaaccgagcc tgattatccg gtaatagtgg tggggataaa tgaaagaata
    42421 gagtataata aagcgactat agttcaaacc gctgataaga taattgctaa aacagcagaa
    42481 attcgtcaat cagaggaaat attagattca ataaggattg caatagacgg tgaaggatgg
    42541 cctaacggag acccagaaat ttacatagta gcagcaggaa ccaatacgac ttgggataga
    42601 agagaaaggg tgaacttagc aagtgtgaat aaaattaata aatggtaccc tattgatgga
    42661 gctcttttca gttgggagac aaaatattgg ggaacacata ccggatatgg agtacttgaa
    42721 tatgatccag atggatataa ctattccgtt actatcctaa gcgaagatgt tgaagtagaa
    42781 gtcaaaaaaa agagtgatgg aaatgatgag tttattggaa atattagtag tattccttat
    42841 gatcatacta atggagtaat ctggttactt gacaatgcaa aaataggact tagttattga
    42901 tgaaaataat ggaggcggta taaaaatatc gcctccattt atcaattaaa ttgtgagttg
    42961 tctatgttgc taaaattctt cgatactgta ttgactattt caatattgaa cttattattt
    43021 tttttagata cctttgccgc cagcatttcc gg
//
LOCUS       DAOMJU010000007        42068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_747_length_42068_cov_20.422726, whole genome shotgun sequence.
ACCESSION   DAOMJU010000007 DAOMJU010000000
VERSION     DAOMJU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 42068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42068
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_747_length_42068_cov_20.422726"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1062)
                     /locus_tag="PLO58_01315"
     CDS             complement(34..1062)
                     /locus_tag="PLO58_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQO73458.1"
                     /translation="MYNIALIGCGRISKNHFESINQLSNDLKLVAVCDIIEERARAAG
                     EKYGVDWYTDYDKMLERDDIDIISICTPSGLHPEHGIKAAQKGIHVISEKPMAISLAG
                     ADALIKACDDNKVQLFVVKQNRLNTTSQLLKRAVDKGRFGKIYMAQTNVFWQRPQEYY
                     SQAKWRGTWEFDGGSFMNQASHYVDALLWLLGPADHVMAETATMARKIETEDTGSAII
                     KFRNGAIGTINVTMLTYPKNFEGSITILGEKGTAKLGGVAINKIEKWEFEDYDDDDRL
                     VHEANYNPPNIYGLGHLPYYKNVIAVLNGDEEANTDGRSGRKSLELIIAIYKSAQTGQ
                     KVALPLKL"
     assembly_gap    1367..1466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1467..1976)
                     /locus_tag="PLO58_01320"
     CDS             complement(<1467..1976)
                     /locus_tag="PLO58_01320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQO73459.1"
                     /translation="MKYFVHKSSYIDDNVEIGEDTKIWHFCHIQSGAKIGRGCSIGQN
                     VNVGNNVVIGNFVKIQNNVSVYEGVTLEDYVFCGPSMVFTNVIDPRCKYPQRGAEFYH
                     KTLVKEGASIGANATIICGNTIGRHAFIGAGAVVTKDVPDYALMVGVPARQIGWACEC
                     GEILPKFDKE"
     gene            complement(2159..>2617)
                     /locus_tag="PLO58_01325"
     CDS             complement(2159..>2617)
                     /locus_tag="PLO58_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQO73460.1"
                     /translation="SHQKYYHSLIGGNFRLDTIQAAALLVKLPHLKSWHLARQKHAEY
                     YDKAFSNSIIKAPYVAYQRSYHIYNQYVILVPDKRNELRQYLADNEIGSEIYYPVPFH
                     LQECFKYLGYNCGDFPHSEYAASHSLALPIYPELTEEMQAYVVEKVLKFY"
     assembly_gap    2618..2717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2718..3211)
                     /locus_tag="PLO58_01330"
     CDS             complement(<2718..3211)
                     /locus_tag="PLO58_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQO73461.1"
                     /translation="MKVPLLDLKAQLTTIRSEMLNAITAVLDSTQYIMGPPVADLEKK
                     IADYCGVQFAVGVSSGTDALLVALMALDVKPGDLVITTPYSFFATAGVIARLGARPVF
                     VDIDPRTFNLNPEHLHDWFIKNSRQIKKVKAIIPVHLYGQCADLDPILEIAGKYNIPV
                     VEDAA"
     gene            complement(3691..4833)
                     /locus_tag="PLO58_01335"
     CDS             complement(3691..4833)
                     /locus_tag="PLO58_01335"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQO73462.1"
                     /translation="MNAESRFLRAVCRVALDETENQIAVDNVVFDEDKLYNLGLYHQI
                     LPLIADYRVTLSKIFPAISATFFEKCRINALESAGRLIVYEKFLANLDQLFQAASIEY
                     RLFKGPVLAYELYEFPYQRPFGDLDILIQPESLEKVHTILGELGCELDDDLYTNFPAE
                     IIKKYRFARHYISVKNPLVAIDVHTGLSSRLHPFHFDFADFWSHSRIIKIGDREYQTF
                     DLSHQALYLLYHAFKHYYFKLIWIIDCRQNTKLPDFDKADFQHLIKTYNLTRVWNIYS
                     RVSSDLWNDSPQLFEYPKVRCSGLSYKINTEVILQGGLFTSLARARILLPLIYLPRLS
                     QKLAYLWRQMFPPRAVMWDFYSSPNLKPTWRNYLRLRYKAVCEIFR"
     gene            complement(4958..5767)
                     /locus_tag="PLO58_01340"
     CDS             complement(4958..5767)
                     /locus_tag="PLO58_01340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HQO73463.1"
                     /translation="MSGRTKSEDFQLILAPDHIYNIDCVIGMQKMPQDFVDLIITDPP
                     FAINFKAQRNNYHRTPSRVLEGYTEIPADEYLNFTLKWMSQAYRILKPSGSMYVFSGW
                     NNLKEVLIALDQVGFVTVNHLIWQYQFGVVTRRRFVTSHYHCLYVCKNDRQRKFFPYS
                     RFDKDARDLSGSSLHYRDKEDVWYIKREYWTGKEKTPTKLPTEVIKKILQYSSSEDDL
                     VCDPFLGSGQVAVVSKMLKRHYIGFEINPVYYEFALKRLMPDNDLFNAQAV"
     gene            complement(5829..8228)
                     /locus_tag="PLO58_01345"
     CDS             complement(5829..8228)
                     /locus_tag="PLO58_01345"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HQO73464.1"
                     /translation="METMNGNIKTGIPERSFTETEKEVSLQDYFRIIYRGRWIIGIVF
                     VVVMLLTIYITFTTPPEYQASTTLMLSGRAASTSTLFQNPLLPMSYMKVNNEIEVLKS
                     TALARNVIKALRNSPYADSLYLLETRKYHKKGVGLGDLMGGITSGLQHIFMPSQEEDL
                     MVVDTLDRAIVKNLMTSMKVEPIRETEAIRLTISSVDPIEAALIANTIASEYYKTDVE
                     YNLGEVIEMKNFLEEQLEKIEKDLKESEEALKNYQRQEGIFGLDETAKILIDQLSTFE
                     ATYYTTLADLKIAQQTLDNQQRMLTEREQMVIQESVNTTNPFVAQLRQSIAEMEAEKI
                     TVMTVQGYSADHPAIKELDKRITDLKQKLFEEAEKITNLGLTPEEQSKISAELLNKVI
                     MNNIEVISLEAKCNEYKKLVDQYSARLDRLPASTLEYARLDRERQVNEKYYILMKTKL
                     QESRITEASKISNVRIIDPAIPPEKPVKPKKKMNLLLGLFLGLGAGVGIAFIRDYLDH
                     SIKSGEDLQRLGLTAIAIIPTIDVKSAVEKIGKVYKDKNNGEEVLVKARLVSHYDPKS
                     AVAEAYRTLRTNIQFMSPDRPIKSLLVTSAGPGDGKSTTVINIAITFANLGKKVLLID
                     ADLRKPIQHKVFEFQRAPGLTHLLIENLPDEEVIHQTEVPNLDLITCGDIPPNPSELL
                     ASQRLKNFMERAREKYDIILFDTPPVIAVTDATVLSQLTDAILLVVRAGSTDLRVLER
                     SIELLRQVKANLVGAVLNGVNMSTGYDSYYYYYRYYYYYSDTGAKKKRASRTRRHHHQ
                     A"
     gene            complement(8238..9260)
                     /locus_tag="PLO58_01350"
     CDS             complement(8238..9260)
                     /locus_tag="PLO58_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQO73465.1"
                     /translation="MKNNSSNIWKKLISPKTLFAFAISILGLYLGFRKFDSSEFLSYL
                     RQTNLWIFGLSMAVMVFLIGLRGWRWKYLILPINDLPVKQVFEMEMIGYFGNHVFPFR
                     MGEILRGYALSKTQNIPVSAVFGTIVLERTLDVLTFIIVLISGAIFFPGMPKWVRTSG
                     LISGIVILILFILVYISNLKKVALKRFFKQKMLKIKNLDDSKIGKTVKNFWQGLTTLR
                     ETPHLGLIIFQSFLIWAVNIGNFWLMGASLQVYFDLFDLLLVFFVTSAIISVPSAPGY
                     VGTYHAATIGILVFLGLELSKAQAVAVIMHAVGFISLTTLGLFYFLKYQLNFKSTVEV
                     YVEKGA"
     gene            complement(9257..9901)
                     /locus_tag="PLO58_01355"
     CDS             complement(9257..9901)
                     /locus_tag="PLO58_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HQO73466.1"
                     /translation="MVTRKYIFNSIILSVLLTIPILLLAQETTQVILKKVAHCYQSAV
                     PFKIGFTVRLAMPDNANLSKYSGIFFLGSDNQFRVSQTEEEIVYDGQWLWTYDKINRQ
                     VIVEEFNPRSSLKLIWDILSGSFDGYQISKSECQNGLLAVVELKPSSKDGYIRSLQMK
                     IDRERGRIVSAEYRDFQDNLVNIELDTLIALGRADTGLFKINLNEGDELIDLRP"
     gene            complement(9961..12177)
                     /locus_tag="PLO58_01360"
     CDS             complement(9961..12177)
                     /locus_tag="PLO58_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HQO73467.1"
                     /translation="MNNRQQEILGILLIALGILIMLSLISYNPIEEPTISKNVAIQNW
                     MGIVGVYISHYLIKYTLGVAVFIFPVLMLVWGWWLFADKERKILIRFSLYSIFMALLV
                     SVALGLPAVRENVDSNFGFRYSGLVGGVFAKVMCDWLGFTGTVIVLLVLALVTIRGYF
                     TWSFYSPIAKWRSHRHPKKERTSGEIPQPRIPDRQFSVSQPEKDAAVVQSETEATRQV
                     KIKKSVEPQPPIIEPKVEPVTKSETSGGVYQLPPIDLLELPPESRDSVTKEEMAENAQ
                     LLENALETFGVKGKVVNVSPGPIITRYEIEPAVGVRVSKIAALADDLARILSAKRIRI
                     VAPIPGKSVVGVEIPNRNPEIVYLRSIIGSRQFASAKSKLTIALGKTTSGEIYTLDLA
                     RMPHLLIAGATGSGKSVCINTIIASLLYLARPDEVKFMLIDPKKLELSIFKALEGYHL
                     ITCEDLDEDVITRADNAIVALRSVELEMERRYEILASATVRNIDEYNNRVVSGELDKE
                     FLPYIIIVIDELADLMLTSAKEVEEPITRLAQMARAVGIHLIIATQRPSVDVITGVIK
                     ANFPARIAFQVASKIDSRTILDLNGAEKLLGRGDLLITTPQNPEPFRVHSAYISLEEI
                     ERILEHIENQPRPGQVELPSVKEESVEEFGLYDDSEKDPLYYEALRLVVTHQQGSISL
                     LQRRLKIGYSRAARLIDELEKASIVGAFTGSKAREVLVDEDYLEQLNKTQNDEDEV"
     gene            complement(12174..13280)
                     /gene="gcvT"
                     /locus_tag="PLO58_01365"
     CDS             complement(12174..13280)
                     /gene="gcvT"
                     /locus_tag="PLO58_01365"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQO73468.1"
                     /translation="MESANNQVKKTPFYDQHLACNAKLVPFAGYWMPIQYRGIMEEHR
                     RVRTTVGVFDVSHMGEFIVRGSSAEDFLNYITINDVSKLAIGQVQYSAMCYPDGGIVD
                     DLLVYRFADHFMLVVNAANLEKDFNWIKQHLPAGVELTDISDETGLLAVQGPLALETL
                     QPLTPINLNEIEFYHFREGEIANVPAILSRTGYTGEKGLEIYHKPADSARLWEAIFQA
                     GAKYDIQPIGLGARDTLRLEMKYALYGNDIDQTTNPLEAGLGWITKLDKPDFIGKAAL
                     LKIKAEGLKRKSVGFEMLEPGIPRHGYKVFKNGQEIGLVTSGTQSPTLNKGIGIAYVA
                     SEYSAVGTPIEIEIRGKLLKAVIVKTPFVPARTQ"
     gene            complement(13290..13883)
                     /gene="ruvA"
                     /locus_tag="PLO58_01370"
     CDS             complement(13290..13883)
                     /gene="ruvA"
                     /locus_tag="PLO58_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQO73469.1"
                     /translation="MFAYISGKLFQKEPTSVIIDVNGIGYQINIPLSTYSALPATGQN
                     AKLLTYYHVREDIQALYGFATPEEKELFLLLISVSGIGPKMAMTILSGTSPEQFRRRI
                     LDGDVESLTLIPGIGLKTARRIIVELGEKMGQTSEVVSGELATVTLGATGEEALRALI
                     SLGYRRAEALNALRKAVKELGDSAPVEKYIKAALNQM"
     gene            complement(13887..14378)
                     /gene="ruvC"
                     /locus_tag="PLO58_01375"
     CDS             complement(13887..14378)
                     /gene="ruvC"
                     /locus_tag="PLO58_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQO73470.1"
                     /translation="MSEELRVIGVDHGINRTGYAIVSKNGSKLSCLTHGSIVTTPREA
                     FNLRLKHIYTELLQIIEAWKPTIMVVEEAIYAQNIKTALLMGHARGAVLLAGANAGLT
                     IAEYPPTKIKSAVVGNGLASKEQVQYMVSRILGLSQEPESFDAADAMAAAICHLNRPN
                     IRD"
     gene            complement(14378..15136)
                     /locus_tag="PLO58_01380"
     CDS             complement(14378..15136)
                     /locus_tag="PLO58_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQO73471.1"
                     /translation="MSGHSKWSTIKRKKGALDAKRGKVFTKIIKEIQVAAKMGGGDEN
                     ANPRLRTAVLAAKAANMPLANVERAIKKGTGEIESVNYEEITYEAYGPGGVAILMEAL
                     TDNKNRTVSELRHILTRHGGNLATTGSVAYNFEKKGIITVNKDTCSEDDLLLIALEAG
                     ADDISTEDDVYEVSVDPSHFEKVKKALESAKIAIASAAVTMIPKTTIKVDEKNAPKII
                     SLMDALEDHDDIQNVYANFDIDDKLLAELQQSAG"
     gene            complement(15167..15649)
                     /locus_tag="PLO58_01385"
     CDS             complement(15167..15649)
                     /locus_tag="PLO58_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQO73472.1"
                     /translation="MKNKAIVVLILLSFACLLAQENIAPQDESFDPAVLKEPPLPMLD
                     NTLIYEVVTDLKPLTKPPVNNAADSIVYIEKMGWKVQVFSTSDYFLADSVYHEIRSQF
                     GDSTVEKVFNLPYYKIRIGNCATREEAERLLVRAQELGYRDAWVIRTQVKLAVKEGLF
                     "
     gene            complement(15724..16488)
                     /locus_tag="PLO58_01390"
     CDS             complement(15724..16488)
                     /locus_tag="PLO58_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73473.1"
                     /translation="MKYLRLLILILSLTVGLIAENPAELDSLFEQGNRAYQEENYPEA
                     IRLYESIIARGYESGPLYFNLANAYYRVNNIGRAILNYERAARLMPNDPNVAFNLQLA
                     NLSVKDRVEAPPPFFLFRWYQNFLNLFSTRGWAVIFSILLLIAASSFAVWLNLDLRRL
                     RRGIKSLFIVSGALSIIVALILFQKYRIETASDFGIVLSGTVSSLSAPQAGSTELFII
                     HEGTKFKILDSDGNWLKIELVDGKQGWVPSADVAKI"
     gene            complement(16490..18319)
                     /locus_tag="PLO58_01395"
     CDS             complement(16490..18319)
                     /locus_tag="PLO58_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HQO73474.1"
                     /translation="MNRLKTFNLLFFLQLICLHALGQDLRISASVNRNNITDKDVLIF
                     SVTLEGISDFPEVPPPESPDFVLISGPSQSSSIQIINGKMTASKTVSWQIAPTRAGKL
                     TINPISIKYRGKIYSTEPVIVNVTASAPTQPRSAPPSGTPDKQVTPSSGEEVFLKAVV
                     PKTTIYMGEELIVSFDLYFKNVRTFGRKKLPDAQGFWKEELDAPTQPVITNETVNGVA
                     YRKATIQQIALFATTTGELTIDPMVVDCEVIQPSQRRSIFDDFFGGSFFDDPMFSKTK
                     IVTVQSEPIKIRVRPLPESGQPASFIGAVGQFKIESKIDTLQVKQDQALTLRYKISGS
                     GNINSIKLPPPSLPNSVEVFEPKIEKKIDKSSGSIKGSVTYEYVLIPRRPGLLTVPAI
                     WFSYFDPQTETYRTISTSSYKVKIVGSEDMLADRSVGLSKTEVSLLGQDIRFIMRENP
                     GWHKSGQTVFSEFWFWLINGFSVLVIIGAITLRWWTEKMESNLTFARRRQAWSKWQNR
                     LRTLQKMADNVVGEDFYAQLSQAVNGYIADRLGLPLAGLGPKEIEQELRQYAIDSELI
                     TKTVKLLEKLDEIRFLPGMIIADDPKELLSSSREIVSALSKVI"
     gene            complement(18327..19046)
                     /locus_tag="PLO58_01400"
     CDS             complement(18327..19046)
                     /locus_tag="PLO58_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73475.1"
                     /translation="MTRSKSISNCISRLSLFALGVVILCPLKISAADSGLRQYKKGNY
                     QEAIRAYDRILKDHDDWEEAHFGKGAALYKSQRIEEARREFEKAISVKDPMRKSAVYY
                     NLGNTLFQSGRIEESLAFYKKALEYNPRDFDAKYNYELARQILQQQKNQDKNQSEQKP
                     DQQNQPEEQPQPDQQSQPQNKEQQEQQAQQQPSQQNRQEKEKSQKEAAQILDALKNDE
                     KKLMQEKMRMKASGLAKEKDW"
     gene            complement(19053..20078)
                     /locus_tag="PLO58_01405"
     CDS             complement(19053..20078)
                     /locus_tag="PLO58_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQO73476.1"
                     /translation="MVKFYHSEFLWFWSLIILAILALIYNRIQHRKSLQQYAGIELSQ
                     KLLEGYRPGLRRLKNNLWLVGLVFLGIALLGPAVGQKLTEVKRRGQDIIIALDTSISM
                     KAEDVKPNRLERAKFELNRFIDGLMGDRIGIVAFAGTSYLQCPLTLDYSAAKLFLDAC
                     DTGIIGTQGTAIADAISTSLKAFKSGEKKHKVIILISDGEDHEGDINQIAKQAAEEGV
                     VIYAVGIGSLTGAPIPLRNITTGEVEFKKDRAGHIVMTALEEETMQKIAAMTGGKYYN
                     LSANKDAFQKIAHEISGMDKKNLQSHEYSDYQQRYQIFLLIGLVLLTTEILIPEKRNS
                     TSRDTNS"
     gene            complement(20078..21070)
                     /locus_tag="PLO58_01410"
     CDS             complement(20078..21070)
                     /locus_tag="PLO58_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQO73477.1"
                     /translation="MFYFANWGFLVLLILVPVLAIWYWKRGHKNAGALRYSSIKNILQ
                     VKNRHAFYKVHGPFVAKLIGIALLIMALARPQSGNTRREFTTEGIDIMLVLDISSSMK
                     AIDFRPNRLEAAKSVAQKFIEGRQSDRIGLVVFAAESFLQCPLTIDYDVLKDLLNQVD
                     IVEEKYDGTAIGMAIANATNRLRESQAKSKIMILLSDGCNNAGEIDPRTAANLANAFD
                     IKIYTIGAGKLGQAPYPIELPNGQIQYRLVNAEIDEVLLQDIANQTGGKYFRATDEKS
                     LAMIYEEISGMEKTEVKVHEFVNYNELYPYFLVPGFLLVALSGLIGLTIWRRTP"
     gene            complement(21089..22465)
                     /gene="gatA"
                     /locus_tag="PLO58_01415"
     CDS             complement(21089..22465)
                     /gene="gatA"
                     /locus_tag="PLO58_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HQO73478.1"
                     /translation="MAGTVFLLGIEHLLERIKPNPYNAFISVAPDIAEKYDEINYKIQ
                     NGQAGSLAGYTIGIKDNICVSGLPTTCASRMLANFMPPYEATVVTKIRQADGLIVGKM
                     NMDEFAMGSTTEFSAFGLVNNPHNEKRVPGGSSGGSAAAVAGNLVDIALGSDTGGSIR
                     QPASFCGVIGLKPTYGRVSRYGLVAFASSLDQIGTFSRNVADSARLLQVIAGRDENDS
                     TTADEPVPNYQNALGKDVRNLRIGVPTEYFSKGLDPEIANRIKQCIQFLEKSGAIVTD
                     ITLPHTPYAIATYYIIATAEASSNLARYDGIRYGLSQRGGDLDTLYRETRHAGFGAEV
                     TRRIMLGTFVLSAGYYEAYYDKAQRVRRLIKEDFVEAFKKVDAILTPTTPTTAYEIGS
                     KIDDPLAMYLGDIYTVPANLAGVPALNFPLGKSSEGLPIGAQLIGRYFDEETILRIAH
                     YIERNYEE"
     gene            complement(22471..22698)
                     /gene="tatA"
                     /locus_tag="PLO58_01420"
     CDS             complement(22471..22698)
                     /gene="tatA"
                     /locus_tag="PLO58_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQO73479.1"
                     /translation="MGNIGTGEIILILIVFLLLFGAKRLPELAKSIGKSLREFKKAAS
                     DIQDEIKDVDSKPKNNSGNSTNPTEQPTPKE"
     gene            complement(22713..22988)
                     /locus_tag="PLO58_01425"
     CDS             complement(22713..22988)
                     /locus_tag="PLO58_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HQO73480.1"
                     /translation="MDSTKRKLSILFVIGILLLGAMIGSLLGELLRSLIPDGVVKEVF
                     LRSIDIMLGPAVIDLIMFSITLGFTLKLNFIGIIGLAVAYYILRFWR"
     gene            complement(22990..23676)
                     /gene="purQ"
                     /locus_tag="PLO58_01430"
     CDS             complement(22990..23676)
                     /gene="purQ"
                     /locus_tag="PLO58_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HQO73481.1"
                     /translation="MKFGVVVFPGSNCDHDAYHVIKHVLGARVEYIWHKDTTLRLFDA
                     IILPGGFSYGDYLRAGAIARFSAIMPAIIQFAARGGKVIGICNGFQVLLEAGLLPGAL
                     IPNDHMRFRCQEVYLKVINKATAFTSEYSEGQVLKMPIAHYCGQYFADDDTLVQLQDQ
                     GRIVFQYCDSDGEVNPATNPNGSKLNIAGILNEDKNVLGMMPHPERAAEQVLGSVDGL
                     GIFKSLLGKD"
     gene            complement(23676..23930)
                     /gene="purS"
                     /locus_tag="PLO58_01435"
     CDS             complement(23676..23930)
                     /gene="purS"
                     /locus_tag="PLO58_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HQO73482.1"
                     /translation="MWLAKVVVSYKEGILDPEAKTIQKALKTLGYQNIEELSVGKYFE
                     IKLNSNLERSDVEKLVREICDRVLSNPVIQRYNYEIVETE"
     gene            complement(23931..>24449)
                     /locus_tag="PLO58_01440"
     CDS             complement(23931..>24449)
                     /locus_tag="PLO58_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73483.1"
                     /translation="DMVSFCLVPAVLIHEIFVSELGFIGGLISFFPLLFGGVRLARFN
                     LQAGAGKKSYFIGMPTPTSALTIGSYIWFSYKVLGNYGNPKIILPLVVVLSFMMVSNI
                     RFSVNLKLGFRRGLPTAFKTGMIILMTIGMVIFNGYVIFPLVMVYIITHLLQWLIGYD
                     EPRIHLARRKAH"
     assembly_gap    24450..24549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24550..25136)
                     /locus_tag="PLO58_01445"
     CDS             complement(<24550..25136)
                     /locus_tag="PLO58_01445"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HQO73484.1"
                     /translation="MIAKESLRLIIPMLIVAVLAIFLAKYFDLGIMMVLFWVTLVSII
                     FTLYFFRDPERITPQNPAFLIAPADGKVVGISEVNDPFVGGGIKIAIFMSPFDVHINR
                     APYSGIIRQIEHKRGKFHSAFKPEAAIENEQCRITMEKDNLKLAVTQIAGFCARRIIS
                     RVKVSQNLEKGERFGLIMFGSRVELIIPKGLKINI"
     gene            complement(25133..25846)
                     /locus_tag="PLO58_01450"
     CDS             complement(25133..25846)
                     /locus_tag="PLO58_01450"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQO73485.1"
                     /translation="MIKNEKLYEGKAKIVYSTDDPELVIQFFKDDATAFDGIKKGQIS
                     GKGVANNLISCQLFSLLENNGIATHFIKKLSDNEMLVRRVKIIPVELVVRNVAAGSLC
                     KRYGVPEGQVFSEPIVECYYKNDAFHDPAMNDDHIIRFNLANADELSAIKATALRINQ
                     ILKEYFDSIGIILVDFKLEFGRYNDQILLADEISPDTCRFWEKGTNRKLDKDRFRFDL
                     GEVEEAYTEMLKRITGIQK"
     gene            complement(25833..27143)
                     /gene="purB"
                     /locus_tag="PLO58_01455"
     CDS             complement(25833..27143)
                     /gene="purB"
                     /locus_tag="PLO58_01455"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQO73486.1"
                     /translation="MIERYTRPAMGRIWSEENKLATWWRVELEVCRVQNERGIIPDEA
                     LQEIESRAKFSLKRIQEIEEEVKHDVIAFLTNISENVGPAARFIHKGMTSSDLLDTAL
                     ALQLSEAGQLILADLNQLEKILTEKSIEYKDCLMIGRTHGVHAEPITFGFKLIIFREE
                     IRRHIKRFELALDDIRVGKISGAVGTYQHIEPEVEERVCHNLGLEPAPVTNQIIQRDR
                     HAFFIATLALIGTTLEKIATEIRHLQRTEVLEVEEFFSEGQKGSSAMPHKRNPVLSER
                     ICGMARLLRGYAVTAMENIPLWHERDISHSSNERVILPDACICTDFILNEMSKVLTNL
                     LVYPENMRRNLEGMHGLIFSQEIMLAIIEKGASREEAYTIVQNNAMKVWQSGSDFKEL
                     LLKDENVLKWLNPAEIESYFNYEKVLTRINRIFERISEGNYDKE"
     gene            complement(27147..28247)
                     /locus_tag="PLO58_01460"
     CDS             complement(27147..28247)
                     /locus_tag="PLO58_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQO73487.1"
                     /translation="MKKINSVLISSLFIGVSLLLAVPQEQQEISNSRRNAITAAIEKV
                     SPAVAGINVTAIEEYVASPFFNDPLWSLLFPEQIYRRRVKSIGSGVVISSDGYIVTNT
                     HVVEGAEEIVVTLMGGKEYKAKLVGIDNISDIALLKIDGKSFTYAKLGSSEDVIIGEW
                     VIALGNPFGLFSVNYKPTATIGIVSGTDLDFGPQQSGRLYQDMIQTDAAINTGNSGGP
                     LVNALGEVIGINTFIFTGGQFSEGSIGIGFAIPVDRVKAVVANLKQYGKDSRTFKIGW
                     ELQNVDSFLARYLNLPSNTGVIVTRVEKGTPAERSGLKVGDVIISINGKKVARAEEIY
                     AYIQENLLRQGDKIKVRFLRGNREYETQLVLE"
     gene            complement(28244..28888)
                     /gene="fsa"
                     /locus_tag="PLO58_01465"
     CDS             complement(28244..28888)
                     /gene="fsa"
                     /locus_tag="PLO58_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQO73488.1"
                     /translation="MKLFIDTANLEEIRKAQSWGLLDGVTTNPTLLSKEKGNFKEILL
                     KICETVNGPVSGEVVSLDAEGMVREAEDLAALHKNMVIKVPITLEGLKAIKILSEKGI
                     RTNTTLIFSPLQAILAAKAGATFISPFVGRLDDIHQVGMELVQQIKTIYDNYGIRTEI
                     IVASIRNPLHIVEAALIGADICTIPFNVMEKMVKHPLTDKGITQFLEDWKKVEK"
     gene            complement(28891..29637)
                     /gene="truA"
                     /locus_tag="PLO58_01470"
     CDS             complement(28891..29637)
                     /gene="truA"
                     /locus_tag="PLO58_01470"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQO73489.1"
                     /translation="MGRRFKIIMQYDGSNYCGWQRQRYQPSVQAKIENALQPLNQNQP
                     VTVIAAGRTDSGVHARRQTAHFDLVTDLSPETIRKALNATLPDDIYIDYCQEVGKEFH
                     ARFTAINRTYHYQVVVNPNVFLRKYVWLVNFRFDRKLLQECAELVKGEHNFTALCRAA
                     TETENKMATIYESRWEEQDELLVYIITANRFLYAMVRMLVGSMMEVARGKYSIGAFKN
                     LLGKNDTKMQVYTAPAQGLFLWNVKYQEDN"
     gene            complement(29637..30383)
                     /locus_tag="PLO58_01475"
     CDS             complement(29637..30383)
                     /locus_tag="PLO58_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HQO73490.1"
                     /translation="MKNLDPRVKILWLIIAVIGIFRLKVWYAQLGVAIILGTIITFRI
                     LYAKENFKVLRILLVFLPLTFLIHILFGTNLLATIFRSAPRDSWSVLLFTPLTFTLRI
                     GNLIALMALATHWLKALEVLDALYHLLKPLRKIGLPVDDIFQIIFIAVRFIPLIQEEY
                     QRLDDSWRIFMNRDSGGLFGQIERLRTILVPLMVVSFRRAEVLAEAMTVRGYNSQVKR
                     SYIGRLHWQTPDWIGLLAGCGSLVLMVINI"
     gene            complement(30385..31071)
                     /locus_tag="PLO58_01480"
     CDS             complement(30385..31071)
                     /locus_tag="PLO58_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73491.1"
                     /translation="MKHLFCPSLLGLLIIFILAGSGTAQIDSTLVAQPDSLPQLTQSQ
                     ARLLLLKSAVLPGWGERSLNYVERSHYFNASEIALWVTYFAFYWYGNAVAADMEAYAA
                     THAGVNPDGKDQYYFTDIGNYENIYAYNEQKLRYRAINLLYPVTPEYYWAWDSESSRR
                     HFDKLRVKSATALRNATFAIGGLVANRLFSMLDVIVLTRNRLEMPDIDIESNWQPYQD
                     GMSFMLSLKF"
     gene            complement(31068..32621)
                     /gene="lnt"
                     /locus_tag="PLO58_01485"
     CDS             complement(31068..32621)
                     /gene="lnt"
                     /locus_tag="PLO58_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQO73492.1"
                     /translation="MMKVKINLWWLILISGVLSGLAFPPLPLGFLAFFALVPLIKAFC
                     DDEFHNGFEKGLVYGLVLNLAIMYWLALNKGTTWYWATLSMVAAVIFLAMNYAIIGLS
                     VGFIGRRLGRNVGIWSFPIIWTAIEYLRSFGSLGFTWNNLCYTQSHAIQLIQSAAVVG
                     PYGISGWIVVINVLIFRLLENLEDYRQVSRRMVIIIALFLIPAIYGMVVLQKPSDEND
                     RRLVNVGLIQPNIDPNAKWDQESFKKNMQLLHDLTDSVATQPLDLVVWPETATPTFLR
                     RNHYAVLTEIREHIERLEVNLLTGTPDLERAGEDYRYYNATFLLRPHSNRIEDYRKIH
                     LVPFGEYIPLSGYFPELSNLNLGQGNFQAGKEVKLFQIPLKVDQRPVSDTVLTFNSVI
                     CYESTFPDLVRQATRQGSELLVIVSNDAWFGNTSAPFLHAEIGRWRAIENRIPVVRSA
                     NTGISLILDQYGRNLQSLPFGKSGWLSAPVTQGNPQTIFVRYGNWPARLCVILALLLI
                     GYSIIRRKK"
     gene            complement(32670..32975)
                     /locus_tag="PLO58_01490"
     CDS             complement(32670..32975)
                     /locus_tag="PLO58_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQO73493.1"
                     /translation="MKVKTYTKKDVVRRTAEALDTKLVDTAEIVDGVFMVLREMLSES
                     DPRIRIEIRNFGVFEVKPTKAKPKARNPRTNEEVYVPARRKTHFKPGRLLKERMKKA"
     gene            complement(32994..35174)
                     /locus_tag="PLO58_01495"
     CDS             complement(32994..35174)
                     /locus_tag="PLO58_01495"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQO73494.1"
                     /translation="MATEILNKVLPRAKQRPQKFLTLISKLNQRAPLTKDEEDFLWEV
                     YSFARNSHRGQRRQSGDSFFEHPYHTALILADLHMDPITVAGGLLHDVIEDTNVTVND
                     IAEKFGDDVAKLVEGVTKISGITFRNRQEEQADNFRKMLLSVAEDIRVLIIKLADRLH
                     NMQTLDSLPPIKQRRVAIETRDVYAPLAHRLGMFKLKSEMEDLVLKALDPDAYRFLEK
                     KVNEKRSEREKYIKVFTQPIIKALNEQGIKAKIVGRPKNFYSIYKKMKTQNKPFEEIF
                     DLLAVRIIVDTKENCYTVLGIVHDLFTPVMDRFKDYIANHKANFYQSIHTTVYGPGGR
                     MVEIQIRTTEMDEIAEEGIAAHWRYKEGRDKPDDIDKYVKWLRDLIEILQTESTGAKD
                     FMDTLKIDLFKDEIFVFTPMGDLIRLPKGATPVDFAYEVHTEIGTRCIGAKVDGKMVP
                     LNSELRSGQTIEIITSDSHRPSYAWLKFVTTAKAIGAIKRYLRKAQFEESINLGKEIL
                     EKENQRNKELRFLKTVQESFEELGFSELDKLYAAVGSGILTIPQIYAKLFPQKEEQLV
                     RQDLDELFIETARKSVKGIKVQGISNLMVSFAKCCNPIPGDQIIGFVSRGRGVIIHRS
                     DCSNIPSLLEDDERLLNVEWDIAGTQAFLVRLKIMAQERKKFLKDVTDIMASTDTNII
                     SVSGDVDESIIHLNLVIQVENLRHLNKILAKLQNVQGIISVERK"
     gene            complement(35174..36553)
                     /gene="dnaB"
                     /locus_tag="PLO58_01500"
     CDS             complement(35174..36553)
                     /gene="dnaB"
                     /locus_tag="PLO58_01500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQO73495.1"
                     /translation="MALNDGEIIRVPPHSDEAEVSVLGAMMLDKEAVSKALQYLDYTA
                     FYKEAHQHIFQAMTDLFNEGQPVDQVSVIDQLKRKKMLEQVGGAYYVTGLVEATPSAA
                     NVEYYANIVLDMAILRRLIVASNEIQTESYEARDSVQDILDTAEQKIFAISENRMKGG
                     FKNFVDVLNRTFEHIDSIHSRKGHTSGVPTGLLDLDELTSGFHPGELIILAGRPGMGK
                     TALALTIARNAAVEYDMPVAFFSLEMADYQLALRILCAEARLDSHLVRKGKLPKEQWQ
                     RLSMQTGRLANAKIYIDDSPVLTMMDIRAKARRAKAEHDIQLVIIDYLQLIKGHRAEN
                     RQQEITEISRGLKALSKEIGVPVIALSQLSRAVEGRADHRPQLSDLRESGSLEQDADV
                     VIFIYRKSFYDKREGSAASDEDERLAEIIVAKQRNGPTDTVEVIFIDKYTRFENKGAQ
                     REEIITTPF"
     gene            complement(36554..37231)
                     /locus_tag="PLO58_01505"
     CDS             complement(36554..37231)
                     /locus_tag="PLO58_01505"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQO73496.1"
                     /translation="MTRLYDDIKTFFKQQAELFPPEIYLISENGEESTPKTDAATQLA
                     ELERICQNCRQCHLSETRTKVVFGTGNPHAKLLFIGEAPGVNEDLQGLPFVGRAGNLL
                     DKMLKAIGLTRAQVYIANVLKCRPPENRTPNVGEVEKCEPYLLKQIAIIQPELIICLG
                     LTSAKTLLRVEYTLEKMRGQIFQYHNVDLIVTYHPAALLRNENLKKYAWEDFKKIKNL
                     YLDKTGE"
     gene            complement(37232..38440)
                     /gene="coaBC"
                     /locus_tag="PLO58_01510"
     CDS             complement(37232..38440)
                     /gene="coaBC"
                     /locus_tag="PLO58_01510"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQO73497.1"
                     /translation="MNRKIVLGLTGGISIYKSAGLLRRLVNDYKADVTVIMTDAAQKF
                     MSPLIFETFCHKPVLTDMFTGQKIETRHIDLATEADLILVSPATANIVAKAANGIADD
                     LLSTIILVGGNKTVFALAMNDNMYANPITQENISRLRRLGYDIIEPEEGALACGSTGR
                     GRLADETTILTHIDRRLNGNKWLKDKKVIVTAGPTREYIDPIRFISNRSSGKMGYALA
                     EIAFRESADVLLISGPSDLKNTVGVKTIRVETANEMRSAVLTEAAEADFLFMAAAVED
                     LSPVQIATQKIKKESGISELKLKYSPDIIMDFRKHNRQACVVGFSVEIESAEHPGAKA
                     RSLAKLNDKEMDFIVWNDPNVTGAAFGHETNSVTLLSKDGQEWVFPLASKRDIADSII
                     STVIKNYKKT"
     gene            complement(38433..38753)
                     /gene="rpoZ"
                     /locus_tag="PLO58_01515"
     CDS             complement(38433..38753)
                     /gene="rpoZ"
                     /locus_tag="PLO58_01515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQO73498.1"
                     /translation="MTETLPFSKLTRKSDDIFELVVVAAKRARQINALRMAQYPLPTM
                     VEEQEETFEETPPEITDEPAWDYLEKPTTLAINEILEGKINYRYMTPEKEKPLEEFDS
                     EFSE"
     gene            complement(38750..39319)
                     /gene="gmk"
                     /locus_tag="PLO58_01520"
     CDS             complement(38750..39319)
                     /gene="gmk"
                     /locus_tag="PLO58_01520"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQO73499.1"
                     /translation="MKPKGLLVAIAAPSGTGKTTVCHELMKRDDRFVFSVSCTTRPPR
                     PNERDGVDYHFISREQFLKNIHEGKMAEWQEIFGNYYGTLKDSIEQALRDGKILLLDL
                     DVKGTLNLKKNYPDQLISIFLLPPSDTELDRRLQKRGTDDSSSIALRKARIPDELELS
                     EQFDFQIVNDNLEATVNKIINIIEEHLKK"
     gene            complement(39316..40260)
                     /locus_tag="PLO58_01525"
     CDS             complement(39316..40260)
                     /locus_tag="PLO58_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQO73500.1"
                     /translation="MAILNLTVWTGLTNFTIDLIGDLQLESMTGYSRTEYRSGGQVIS
                     VEIYSENNRFLDLTFNLPEPLEPYTEELKRLVAGDLRRGRVRITIAITGSEKTPEVQL
                     NTAFLTKYYRALCDLARELQIEEPVTLQNLLTYPEIFITDSADTDVAELLTQVKDLLR
                     QAVAELIAMRRREGEFITRELHSHLQFILEKCSEIEKLSQQSRQEYFTKYQQSLAELC
                     QNCQLDENRLIQEAGILAKKLDITEECTRLRSHIQQFTLLLQSEEPVGKQMNFLAQEM
                     NREITTIGSKSESTAISHLVVSLKNELEKIREQIQNVI"
     gene            40358..42064
                     /locus_tag="PLO58_01530"
     CDS             40358..42064
                     /locus_tag="PLO58_01530"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HQO73501.1"
                     /translation="MPSKQHPLGALINALLALLFNMFNSWLHIERLAREYQQLIGGAI
                     CGMPFTYQKDHLHIPLEMAGEWHCLHFSVQAPLPFFTLERTLPQPKHKVNLLRDVTAK
                     AITDLVCRENDRQVLITLNSGIAYLLFHLYGINGNVYLLDSSGVSRDSFKKAKKPVDF
                     ELSNFRPANRLFPDYADFTSSLRQHSDQSLMTFFTKSFQPILPKILVTEICYRTNLAP
                     QYSIINLTDEQVERLAKTIAELSDEIVTSLPRIYSGEPPIFSLIELQSLSNREFAEYD
                     SVIAAQNHFIQDYLNLYRLKEKKKTLLRQTEFLLSTTRRKLIKQQSELAALPTADNYR
                     EWADTLMATASTIPHHSNSVILPQLTDEARQITIPLEPKLNPIQNAERYYAKSRQIEK
                     ARAELIQKIAEAEQLIQRLENTQKQIAEADDFKTLRSISSHWPAQPQSSIENIHQPFI
                     NINLDGWEILIGKSARDNDELLQKFARPNDFWLHAQGTSGSHIVVRNPGKVASLPRPI
                     LEKAAGLAAFHSKAKHSAVVPVIFTQCKYVTKPRHSAPGAVTVKFEKTIMAEPLDPKK
                     FL"
BASE COUNT        11802 a         9644 c         8397 g        11925 t
ORIGIN      
        1 cttctcctct acctctacct cgaccttaac ctcttatagc ttcagcggta gggcaacttt
       61 ctggccagtt tgggctgatt tatagatcgc gataatcagt tcgagactct tgcgaccact
      121 gcgtccgtcg gtgttggctt cctcatctcc attcaaaacg gcaatcacat ttttataata
      181 aggtaaatgt cctaagccgt aaatatttgg tggattgtaa ttggcttcgt gaaccaaacg
      241 atcgtcgtcg tcataatctt caaattccca tttctcgatt ttgttgatgg ctacgccgcc
      301 aagtttagcg gtgccttttt caccaagaat tgttatagaa ccttcgaaat tcttgggata
      361 agtcaacatc gttacgttga tcgttccaat tgcgccattg cggaatttaa ttatagccga
      421 gccggtatct tcggtctcga ttttgcgtgc catagtagcg gtttcagcca taacgtgatc
      481 cgctgggcct aacagccaga gtaacgcatc aacataatga ctggcttgat tcataaatga
      541 gccgccgtca aattcccaag tgccgcgcca ttttgcctgc gaataatatt cctgcggtct
      601 ttgccagaaa acattcgtct gcgccatata aatttttcca aaacggcctt tgtcaacggc
      661 tcttttaagt agttgggaag tagtatttag ccgattttgt ttgacgacaa ataactgaac
      721 tttgttatcg tcgcaggctt taatcagggc atcagcgccc gccagtgaaa tagccatcgg
      781 tttttcggaa atgacatgaa tgcctttctg agcggcttta atgccgtgtt ccggatgcag
      841 accgctgggc gtacagattg aaataatatc aatatcatct ctttcgagca ttttatcata
      901 atcagtgtac caatcaactc cgtatttttc accggcggcg cgggcgcgtt cctcaataat
      961 atcgcagacc gctactaatt tcaggtcatt agacaattga ttaatggatt caaagtgatt
     1021 tttagaaatt cgaccgcaac cgataagagc aatattatac atcatttcct cgttttattt
     1081 tctcgcaaag aacgctaagg tttttttatt tattacattc tgctcttggc gtcacttggc
     1141 gtccttggcg gtttcatcct tgtttaaccg caaagaacgc aaagtatttt attaagcctt
     1201 tatagtctat ttctcaccaa gcatgtaaag tttcaaacgg acacaaagta tttttatata
     1261 gtttttaaat tattaactat gcgagtaata ccatctttta gatgggcttc attaaaattg
     1321 attaacaaac caagatgtct ccctgacaat cgcaaataag ttaagannnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnttct ttatcaaatt ttggtagaat ctcgccgcat
     1501 tcgcaagccc agccgatctg acgggcaggt actccgacca ttaaagcata atcgggaaca
     1561 tctttcgtca ccactgctcc ggctccaata aaagcatgac gaccgattgt atttccacaa
     1621 ataatcgtag cattagcgcc gatcgaagcg ccttccttaa ccagagtttt atgataaaat
     1681 tccgcgcctc tttggggata tttacaacgc ggatctatta cgttcgtaaa aaccatcgaa
     1741 ggtccacaaa agacataatc ttcgagggta acaccttcat agaccgagac attattctga
     1801 attttcacaa aattgccgat tacaacgttg ttgccgacat tgacattctg cccaatcgaa
     1861 cagccgcgac cgattttcgc accggactga atatggcaga agtgccagat tttggtatct
     1921 tcgccgatct cgacattatc gtcaatgtaa ctggatttgt gaacgaaata tttcatactc
     1981 atgttttttt ggtaaagaag ataaaaactg aatagtatta accggaaaat tcaataattt
     2041 ttcgtgctaa tctacttcct aatttaatcc gccattcttt tactgaattt tcagatgctt
     2101 tctctatttt ggactttatt aaacctgtcg aatatttcta ctttaacttt taaagggctt
     2161 aatagaactt gagaactttc tccaccacat aagcctgcat ttcttccgtc aactccggat
     2221 agattggcag agccagggaa tggctggcgg catattcgct atggggaaaa tcaccacaat
     2281 tataaccgag gtacttaaaa cattcctgca gatggaacgg caccggataa tagatttcgg
     2341 agccgatttc attatcagcc aaatattgac gtaattcatt ccttttatcc ggcaccaaaa
     2401 tgacgtactg attataaatg tggtaggatc tttgataagc gacataaggc gcttttatta
     2461 tagagttact aaaggcttta tcgtaatatt cggcatgctt ttgtcgggcg agatgccagc
     2521 tctttaggtg cggtaatttc accaataaag ccgcggcttg aatcgtgtcc aacctaaaat
     2581 tgccaccaat tagtgagtgg taatatttct gatgggannn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnngcg gcgtcctcga ccaccggtat attgtacttg cccgcaattt
     2761 ctaaaatcgg atcgagatcg gcacactgac cgtatagatg aaccggaata atggctttaa
     2821 cctttttaat ttgccttgaa tttttgataa accagtcgtg cagatgttct gggttgagat
     2881 tgaatgtgcg aggatcaata tctacaaaaa ctgggcgggc tcccaaacga gcgataactc
     2941 cagccgtcgc aaaaaaagaa tatggcgtcg taatgaccag atcgccgggt ttaacatcca
     3001 aagccattaa agcaactaac aaagcgtcag ttccggaact aacgccaacg gcaaattgca
     3061 caccacagta atcggcaatc ttcttctcga ggtcagccac cggtggaccc ataatgtatt
     3121 gcgttgaatc caataccgcc gtaatggcat tcagcatttc agaccgtatg gtagttaatt
     3181 gggcttttaa atcgagcaag ggcactttca tagtttcttc ccaaataaag taattaattt
     3241 caatgagata ttagtctttt atttactggc ggttagaatt tggcgccgaa cctgagttac
     3301 cgattccttt ccaaaggctc cgccccgtca actacattca gcgacgaggc caagaaataa
     3361 atacatcgaa tttcagaata ttttgcgtta attactaaac ttctgtcgtg acttctctca
     3421 caaaagcacg gatttcgcta tatgcttccg gtgattacca acttccaaaa tcaataaatg
     3481 gaaaggattt tatgtcaatt tcatgtcatt ttcagttagc aatattccct gtatttctca
     3541 atatcactca aacaaagtaa tttcaaaaaa aatagcatca attccaatat aaaaaattga
     3601 gtccccaacg aattaattga tatagatcgt taaaatttgt tacatgcaat taaatcttcg
     3661 gtaaaatttt aagaaatttc caccgatcta ctatctgaaa atttcacata ccgccttgta
     3721 tcgtagccgc aagtagttac gccaggtcgg cttcaaattc ggagaagagt agaaatccca
     3781 catcacagcg cgcggcggaa acatttgccg ccataaatat gctaattttt gagacaacct
     3841 tggtaaataa atcagcggca gtaagattcg ggctcgtgcc agcgaggtaa acagtccccc
     3901 ctgtaagatt acctctgtat ttattttata agacaatccc gaacatctta ctttaggata
     3961 ttcaaacaat tgcggtgagt cattccacaa atcagatgaa acccgagaat agatattcca
     4021 tacacgtgtc aagttatatg tcttgattag atgttgaaaa tcggctttat cgaaatcagg
     4081 caatttagta ttttgtcggc agtcaataat ccaaatcaac ttaaaataat aatgcttaaa
     4141 cgcatgatat agcaaataaa gagcttggtg agatagatcg aaagtttgat attcacgatc
     4201 gccaatctta attattctgg aatgcgacca aaagtcagca aaatcgaagt gaaacgggtg
     4261 caagcgactg cttaagccag tatgcacatc aatcgccacc aatggattct ttaccgaaat
     4321 ataatggcgg gcaaatctgt acttcttgat gatttctgca ggaaaattag tgtaaaggtc
     4381 atcatccaac tcacaaccta attcacctaa tatcgtatga actttttcca gtgattcagg
     4441 ctgaattaaa atatccagat cgccaaaagg gcgctgataa gggaattcat acaattcata
     4501 agctaagact ggtcctttga acaaacggta ttcgatgctt gcggcctgaa atagttgatc
     4561 gagattagcc agaaattttt cataaactat aagtcggccg gcactttcca gagcattaat
     4621 tcggcatttt tcgaaaaatg tggctgagat ggctgggaaa attttggata gagtcacacg
     4681 ataatcggca atcagcggta gaatctgatg gtataatcca agattataga gtttatcctc
     4741 atcgaaaacg acattatcaa cggcaatttg attttcggtt tcatctaaag caaccctaca
     4801 aactgctctt aaaaacctac tttcagcatt cattgcttag aaatatatac ataaccagat
     4861 agaaaaacac ataaaaatta tttcagctaa taatcacgaa aaggaataaa aggttattga
     4921 aagttagtta aacggaagcc tatggaaaac tgccgattca gaccgcctga gcattaaaaa
     4981 gatcattgtc aggcatcaat cttttaagag caaactcgta atagaccgga ttaatctcga
     5041 agccaatgta gtgacgtttc aacattttac ttactactgc cacctgcccc gatcctaaaa
     5101 atggatcgca aaccaaatcg tcttccgaac tggaatattg caagattttt ttgatgactt
     5161 cagtcggcaa tttggtaggg gttttttcct tgccagtcca gtattcgcgc ttgatatacc
     5221 agacatcttc tttatcgcgg taatgcaaac ttgagccaga taaatcgcgg gcatctttat
     5281 caaatcggct atagggaaaa aatttgcgct ggcgatcatt cttacagacg taaagacaat
     5341 gataatggga agtgacaaag cgccgtctgg taactactcc aaactgatac tgccagatca
     5401 gatgattgac ggtcacgaaa ccaacttgat caagcgctat caacacttct tttaaattat
     5461 tccaaccgga aaaaacgtac atactcccgc tcggtttcaa aatccgataa gcttgagaca
     5521 tccatttgag cgtaaaattt agatattcat ccgctggaat ttcagtataa ccctccaaca
     5581 ctcgcgacgg agttctatga taattattcc gctgggcttt gaaattaatc gcaaacggtg
     5641 ggtcagtaat tatcaaatca acaaaatcct gcggcatctt ttgcatcccg ataacgcaat
     5701 caatattata aatatgatca ggtgccagta ttagttggaa atcttccgat ttagttcttc
     5761 ccgacatagc agattatcag aatcgccaag tgaacttggt tgtcaatcga ctagccagcc
     5821 tcacataatc aggcttggtg atgatgtctt ctggtacgtg acgccctctt tttcttggcg
     5881 ccggtatcgc tatagtaata atagtagcga taatagtaat agtatgaatc atagccagtt
     5941 gacatattca ctccattcag tacggctccg actaggtttg cttttacctg cctcaacagt
     6001 tcgattgatc tctcaagtac tctcaaatcg gtcgaaccag ctctaaccac cagtaaaatt
     6061 gcatcagtaa gttgcgatag gacggtcgca tcggtaacag cgataactgg cggagtatca
     6121 aacaggatta tgtcgtattt ttccctcgct cgctccataa aatttttaag gcgttgagag
     6181 gctaagagtt ccgaaggatt aggcggaata tcgccgcaag ttattaagtc aagattggga
     6241 acttcagtct ggtgaataac ctcttcatcc ggcaagttct cgattaaaag gtgggttaag
     6301 ccgggagctc gttgaaattc gaatacctta tgttgaatcg gcttgcgcaa atcggcatca
     6361 attagcagaa ctttcttacc cagattggca aaagtaatag caatattaat gaccgtggtg
     6421 gacttgccat cgccggggcc agcactggta acaagcagac tcttgatcgg gcggtcggga
     6481 ctcataaact ggatattagt gcgtagagtg cggtaggctt cggcaacggc cgacttggga
     6541 tcataatggg aaaccaaccg cgctttgacc agtacttcct ctccgttgtt tttatcctta
     6601 taaactttgc caattttctc gacagcgctt ttgacatcaa tggtcggaat gatcgctatc
     6661 gccgtcaatc ctaaccgctg taaatcctca ccagatttaa tcgaatgatc gagataatcg
     6721 cgtataaatg caatcccgac tcccgcacct aatcccagaa ataaacctaa aagcagattc
     6781 atcttctttt tgggcttgac cggtttttcg ggtggaatgg ctggatcaat aatgcgaaca
     6841 ttactaatct tactggcttc ggtaatccgc gattcctgaa gtttcgtttt catcaggata
     6901 tagtactttt cattcacctg acgttcacga tccaggcggg catattcgag agtactggct
     6961 gggagtctat caagtcgggc agaatactga tcaactaatt ttttatattc attacatttg
     7021 gcttccaggg aaatgacctc gatattgttc attattacct tattgagtag ttcagccgaa
     7081 attttagatt gctcttccgg cgtcaatcct aaattggtaa tcttttcagc ttcttcaaat
     7141 agcttctgct ttaaatctgt aattctttta tccagctctt tgattgccgg gtggtcagca
     7201 ctataacctt gaacggtcat taccgtaatt ttttcggctt ccatttcggc aatactttga
     7261 cgaagttgag ctacgaaggg attagtggta ttgaccgatt cctggataac catttgctcg
     7321 cgttcggtta gcattctctg ttgattatca agagtttgct gagctatttt cagatcagct
     7381 agagtcgtat aataagtagc ctcaaaagtg ctgagttgat caattaaaat tttagcagtt
     7441 tcatccagac cgaaaatacc ttcttggcgc tggtaatttt tcaaagcctc ttccgactct
     7501 ttcaggtcct tttctatttt ttcgagttgt tcctcgagga agtttttcat ctcgatcact
     7561 tccccaaggt tgtattctac atcggtctta taatattcac ttgcgatcgt attagcaatt
     7621 agggcggctt ctatgggatc aacggaagaa attgtcaacc gaatagcttc agtttcgcga
     7681 atcggttcga ctttcataga tgtcattaaa tttttgacaa tagctcgatc gagggtatca
     7741 actaccatca aatcttcctc ttgggacggc ataaaaatat gctgtaatcc gctggttatg
     7801 ccgcccatca gatctcctaa gccaacgcct tttttatggt attttcttgt ttctaaaagg
     7861 tatagactgt cagcataagg cgaattacgt aaggctttga ttacatttcg cgctagagca
     7921 gtgcttttca acacctctat ctcgttattg actttcatat aactcatcgg taacaatgga
     7981 ttctggaata aagtgctggt agaagccgct cggccggaga gcatcagagt cgtagatgcc
     8041 tgatattccg gcggagtggt aaatgtaatg taaatcgtga ggagcataac cacaacgaaa
     8101 acgattccga tgatccagcg gcctcgataa ataatccgaa aataatcctg aagcgaaact
     8161 tccttttctg tttcagtaaa gcttctttcc ggaattcctg ttttgatatt accattcata
     8221 gtttccattg agtaatccta tgctcctttc tcaacataca cttcgactgt actcttgaaa
     8281 tttaattgat atttaagaaa ataaaacaaa cccaaagtgg ttagactaat aaaaccgact
     8341 gcgtgcataa ttaccgccac agcttgagct ttcgacaatt ctaaccctag aaaaaccaga
     8401 atgccgatcg tcgcggcatg ataggttccc acgtagccgg gagccgacgg aactgaaatg
     8461 atggcagaag taacaaaaaa aaccagcaat aaatcgaaca aatcgaagta aacctgtaag
     8521 gacgcgccca tcaaccagaa attgccgata ttaaccgccc agatcaagaa cgattgaaag
     8581 ataatcaatc ccagatgcgg cgtttctcgc aaggtcgtca aaccttgcca aaaattctta
     8641 accgtcttgc ctattttact atcatctaaa ttttttatct ttagcatctt ttgcttgaag
     8701 aaccttttca gtgcaacttt tttaaggttt gaaatatata ccaaaataaa taaaattaaa
     8761 ataactattc cactaattaa accgcttgtt cgaacccatt taggcatacc cgggaaaaaa
     8821 atagctccac tgattagaac tataataaaa gtcagaacat cgagagtccg ctccaataca
     8881 atagtcccga atacggcgct aaccggtata ttttgggttt tactcagagc ataacccctg
     8941 agaatttctc ccatacgaaa gggaaaaaca tggttaccga aataaccgat catctccatc
     9001 tcaaaaacct gtttaacagg caaatcattt atcggtaaaa tcaaatattt ccagcgccaa
     9061 ccacgtaatc cgattagaaa aaccataacc gccattgaca atccaaaaat ccagaggttg
     9121 gtctggcgca agtaacttaa aaattcgcta ctatcaaatt tgcggaagcc caaataaagt
     9181 cccaaaatac tgatggcaaa ggcgaataac gtcttcggac taatgagttt tttccaaata
     9241 ttactggagt tatttttcat ggccgtaaat ctattaactc atcgccttca tttaaattaa
     9301 ttttgaataa tccggtatcg gctcgcccca aagcgatcag agtatctaat tcaatattaa
     9361 ccaaattatc ttgaaaatca cgatattcag ccgatactat ccttccgcgt tcacgatcaa
     9421 ttttcatctg taaggaacgg atatagccat ctttagaaga cggtttcaat tctacaacag
     9481 ctaaaagacc attttggcac tcacttttac taatctgata accgtcgaag gaaccactta
     9541 aaatatccca aatcaatttt agactggaac gcggattgaa ttcttcaact attacttgcc
     9601 gattaatttt atcgtaggtc cagagccatt gaccatcata tacgatttcc tcttccgttt
     9661 gactcacgcg aaactggtta tcagagccta gaaaaaagat accgctgtat ttactcaaat
     9721 tggcattgtc cggcattgcc agtcggaccg taaacccgat tttaaacggc accgctgatt
     9781 gataacagtg cgcgaccttt ttaagaatca cctgagtggt ttcttgagct aataataata
     9841 ttggaatagt cagtaaaaca gacaagatta tagaattgaa aatatattta cgagttacca
     9901 atttcacggc aataatttaa gaagttttca gttagtagct ttgatattta tcacagaatt
     9961 tcagacctcg tcttcgtcat tttgagtttt attcaattgc tccagataat cttcatccac
    10021 taaaacttcg cgagctttac taccggtaaa cgccccgacg atgctggctt tttccagttc
    10081 atcaattaac cgagcggctc gggaataacc gatttttagt cggcgttgga gaagagaaat
    10141 cgaaccttgc tgatgagtta cgactaaacg caaagcttca tagtagagcg ggtctttctc
    10201 actgtcatcg tataagccaa attcttccac actttcttct tttaccgagg gcagttcgac
    10261 ctgacccggt cggggttggt tctcaatatg ctccagaatt cgttcaattt cttccagtga
    10321 aatataagcg ctgtgaactc ggaaaggctc gggattttga ggtgtggtaa ttaacagatc
    10381 gccgcggccc agtaattttt ccgcaccatt taaatccaga atcgttcgcg aatcaatttt
    10441 tgaagccact tgaaaggcga tacgagcagg gaaattagct ttgataacgc cggtgatgac
    10501 atcaactgac gggcgctggg tggcaataat caaatgaata cccaccgccc gtgccatctg
    10561 agctaagcgc gtaatcggtt cttcgacttc tttggctgag gtgagcatca aatcggcaag
    10621 ttcatcaatt acaatgataa tatacggcag gaattcttta tccagttcac cactgaccac
    10681 ccggttatta tattcatcaa tattgcgtac ggtagcactc gccagaattt catagcgccg
    10741 ttccatttct agttcgacgg aacgcaaggc tacaatcgca ttatccgccc gagtaataac
    10801 gtcttcatcc agatcctcac aagtgattaa atgataacct tccagggctt tgaaaatgga
    10861 caattccaat tttttaggat caatcagcat aaatttgact tcatccggtc gagctaaata
    10921 aagaaggctg gcgataatag tgttaatgca gacactttta ccggagccgg ttgcaccggc
    10981 aattagcaga tgaggcatac gcgccaaatc cagagtataa atttcgccag aggtggtttt
    11041 tccgagagcg atggtcagtt tcgacttagc gctagcgaat tgccgtgaac cgattatact
    11101 gcgcagatag acaatttccg gattacggtt gggaatctcg acgccgacca ccgatttacc
    11161 cggaatcgga gctacgatcc ggatgcgttt agcggaaaga atccgcgcca gatcatcggc
    11221 taaggcggcg attttactga cacgcacgcc gacggcaggt tcaatttcat atcgggtaat
    11281 aatcggacct ggcgaaacgt tgactacctt accttttacg ccgaaggttt ccagtgcatt
    11341 ttccagcaac tgggcatttt cagccatctc ttccttagtc accgaatctc ttgactctgg
    11401 tggtaattcg agtaaatcaa taggcggcag ttgatatact ccgcctgagg tctcagattt
    11461 agttactggt tcgacttttg gttcgattat tggcggttgt ggttcaaccg actttttaat
    11521 ttttacttgt cgagtagctt cggtttcaga ttgaacaact gcggcatctt tttccggttg
    11581 cgatacagaa aactgtcgat caggaatacg aggttgaggt atttcgccgg atgttctttc
    11641 cttttttggg tgacgatgcg aacgccattt agcgatcggg ctataaaaac tccaggtaaa
    11701 atagccgcgg atcgtcacca aagccagcac taagagtaca atcaccgtgc cggtaaaccc
    11761 cagccaatca cacattacct tggcaaagac tccgccaacc aagccactat agcggaaacc
    11821 gaagttgctg tccacatttt ctctaacagc cggtagtccc agagctaccg atactaatag
    11881 agccataaaa atagaatata aactgaaccg gattaatatc ttgcgttcct tatcggcaaa
    11941 taaccaccag ccccagacca acatcaatac cggaaaaatg aaaaccgcaa ctccgagggt
    12001 gtatttaatc aaataatggg aaatatatac tcccacaatt cccatccagt tttggatagc
    12061 gacatttttc gagatagtcg gttcttcaat cggattgtag gaaatcaagc tcagcataat
    12121 taaaataccg agtgcaataa gtaaaatgcc gagtatttcc tgctgtcggt tattcactgc
    12181 gttctcgctg ggacgaatgg ggtttttacg attacagctt ttagtaattt gccgcgaatt
    12241 tcgatttcaa tcggcgttcc gacagcacta tattcggaag cgacataggc aattccgatt
    12301 cctttgttta aagtcggcga ctgggttcca ctggttacta gaccgatttc ctggccattt
    12361 ttaaaaacct tatagccatg gcgcggaata cctggctcga gcatttcaaa gccgacactc
    12421 ttacgtttca agccttccgc ctttattttg agtagcgctg cttttccgat gaaatctggt
    12481 ttgtcaagtt tagtaatcca gcccagtccg gcttccagcg gattagtcgt ctgatcaata
    12541 tcgttgccat agagtgcata tttcatttcg aggcggagag tatcacgggc acctaatcca
    12601 attggttgga tatcgtattt tgccccggct tgaaaaatag cctcccaaag tctggcactg
    12661 tcagccggtt tatgataaat ttccagccct ttttcgccgg tataaccagt ccgcgacagg
    12721 atagccggaa cgtttgctat ctcaccttcc cgaaaatgat aaaattcaat ttcatttaaa
    12781 ttgattggcg ttaatggttg taaggtctca agggctaacg gaccttgaac agccagaagt
    12841 ccggtttcat cggaaatatc agtcaattcc actcccgcag gaagatgttg cttgatccag
    12901 ttaaaatctt tctccagatt ggcggcattg acgaccagca taaaatgatc agcaaaacga
    12961 taaacaagta aatcatcaac gattccaccg tcgggatagc acattgcaga atattgcact
    13021 tgaccgattg ctaatttgga cacatcattt atcgtaatgt aattcaaaaa gtcctcagcg
    13081 gaggaacccc tgacgatgaa ttcacccata tgggaaacat cgaaaactcc gactgttgtt
    13141 cggacgcggc ggtgttcttc cataataccg cgatattgaa tcggcatcca gtaaccggca
    13201 aaaggcacaa gtttggcgtt acaagccaag tgctggtcat aaaatggggt cttttttacc
    13261 tgattgttag cgctttccat atttccgcct tacatttgat taagagccgc tttgatatat
    13321 ttttcaacgg gcgcagagtc accgagttcc ttgacagctt tgcgtagagc attcagagct
    13381 tctgcccgcc ggtaaccgag cgaaatcagg gcgcgtaatg cttcctcgcc agtcgctccc
    13441 aaagtaacag tcgcaagttc accggaaaca acctcagatg tctgtcccat tttttctccc
    13501 aattcaacga taattcgtcg tgccgttttt aatccaatac ccggaattaa ggtcagagat
    13561 tcgacatcac cgtctaatat ccgacgccga aattgttcgg gactagtgcc agagagaatc
    13621 gtcattgcca tctttggccc gatgccggac acgctgatta gcagtaaaaa cagctctttt
    13681 tcctcgggag tagcaaagcc atacaaagcc tggatatctt cgcgaacatg atagtaagtg
    13741 agtaacttgg cattttgacc ggtagctggt agagcggaat acgtcgataa aggaatatta
    13801 atctgataac caataccgtt gacatcaatt atgaccgaag taggctcttt ctggaataat
    13861 tttccactaa tataagcaaa catactttaa tccctgatgt ttggacggtt aaggtgacag
    13921 attgctgccg ccatagcatc agcggcatcg aaggattccg gttcttgcga gaggcctaaa
    13981 atccgactaa ccatatattg cacctgttct ttagatgcta aaccgttacc aactacggcc
    14041 gattttattt tggtgggtgg atattcggca atcgtcaagc cggcattggc gcctgccagt
    14101 aacactgccc cccgagcatg tcccatcaga agcgcagttt tgatgttctg ggcatagata
    14161 gcttcctcca ctaccatgat agtcggtttc caagcttcaa taatctggag caattccgta
    14221 taaatgtgtt ttaaacgcag attaaaggct tcacgaggag tagtcacgat cgagccatgg
    14281 gtaagacaag agagcttaga gccgtttttg ctaacaatgg cgtacccggt gcgattaata
    14341 ccatggtcaa cgccgattac tctaagttct tcagacatta tccggcactc tgttgcaatt
    14401 cagccagtaa tttatcatca atatcgaagt tggcgtaaac attttgaata tcgtcatgat
    14461 cctcgagagc atccattaga cttatgattt tcggagcgtt tttttcatct actttgatag
    14521 tagttttagg aatcatggtt acggcggcgc tggcaatggc aatcttagcc gattccagcg
    14581 ctttttttac cttttcaaaa tgactgggat caacactaac ttcataaacg tcgtcttcag
    14641 tgctaatatc gtcggcgcca gcctccagag caattaataa aagatcgtct tccgagcaag
    14701 tatctttatt cacggtaatg atgcctttct tttcaaaatt ataggcaaca ctgccggtgg
    14761 tagctaaatt tccgccgtgg cgggttaaaa tgtggcgcag ttcagaaacg gtgcgatttt
    14821 tattatcggt taaggcttcc attagaattg ccacgccgcc gggtccgtag gcttcataag
    14881 tgatttcttc gtaattgacg ctttcaattt caccggtgcc ttttttgatg gctcgttcga
    14941 cattagccaa aggcatatta gccgctttag ccgctaagac cgctgtgcgg aggcgcggat
    15001 tagcattttc atcgccgccg cccattttag cggcgacttg aatttcctta ataattttag
    15061 taaaaacctt gccgcgcttg gcatcgagag cccccttttt tcgcttaatt gtgctccatt
    15121 ttgaatggcc tgacatattt aaaacctcat catttttaat attttgttaa aataagcctt
    15181 ctttaacagc cagcttaacc tgagtgcgga ttacccaggc gtcccgatag cccaattctt
    15241 gagcccggac taacaggcgt tcggcttctt cccgcgttgc acagtttcca attcggattt
    15301 tataataagg caaattgaac actttttcaa ctgttgaatc accaaattgg cttcgaattt
    15361 catggtaaac cgagtcagcc aggaaataat cactagtgga aaatacctga actttccagc
    15421 ccattttttc gatataaacg atagaatcag ccgcattatt aactggcggc ttggtcaacg
    15481 gtttaagatc agtaacaact tcatatatca gcgtgttgtc cagcattggt agaggcggct
    15541 ccttcaaaac ggcgggatca aaactctcat cctgaggtgc tatattttcc tgagctagca
    15601 aacaagcaaa tgataataaa attaaaacta ctatggcttt atttttcata ttcgtaaaat
    15661 taacacagcc aaaatatacg gagattatgg gcaattttaa aagagataaa cagtcttagg
    15721 cgtttagatt ttagccacat cagcgctcgg tacccagcct tgtttcccgt caactagctc
    15781 gatttttagc caatttccat cggaatcaag aattttaaac ttggttcctt cgtgaatgat
    15841 aaataactca gtgctaccgg cttgaggggc ggatagactg ctgaccgttc cacttagaac
    15901 gatcccaaaa tcgctggcag tctcgattcg atatttttgg aacaggatca aagccactat
    15961 aatactgagc gctcctgaaa caataaaaag tgactttatt ccgcggcgca gacgtcttaa
    16021 atccagattc aaccaaacgg caaaactact agctgcaatt aagagtaaaa tcgagaatat
    16081 caccgcccaa ccacgcgtac taaatagatt caaaaaattt tgataccaac ggaaaaggaa
    16141 aaatggcggt ggcgcctcga ctcggtcttt gacactcaaa ttagccaatt gcaggttaaa
    16201 agccacattc ggatcattgg gcataagtcg ggcggcgcgc tcgtaattca gaatcgcgcg
    16261 cccgatatta ttgacgcggt aataagcatt tgccaggtta aaatatagtg gaccgctttc
    16321 atagccgcgc gcaatgatgc tctcatacaa gcgaatggct tccggatagt tttcctcctg
    16381 gtaagctcga ttaccttgtt caaacaatga atcgagttcg gctgggtttt cagctattag
    16441 cccgacggtt aaacttaaaa tcagaatcaa taggcgcaag tatttcattt taaatcactt
    16501 tactcaaagc cgatacaatt tcacgactgg aagacagcag ctcttttgga tcatcggcga
    16561 ttatcatccc cggtagaaaa cgaatttcgt ccagcttctc gagtaatttt acggtttttg
    16621 ttatcagttc agaatcaatc gcatactgtc tcaattcttg ttcaatttcc ttgggaccta
    16681 aaccagccag cggtaaaccg agccgatcgg cgatataacc gttcaccgcc tgagacaatt
    16741 gagcgtaaaa atcttcaccg acgacattat ccgccatttt ttgcaaagtc cgcagacggt
    16801 tttgccattt cgaccaggct tgccggcgtc gcgcaaaagt caggttgctc tccatttttt
    16861 cagtccacca gcgcagagtg attgcaccaa ttataaccag caccgaaaag ccattgatca
    16921 gccaaaacca gaattcgctg aaaacagtct gcccggactt atgccagcct gggttctccc
    16981 gcataatgaa acgaatatcc tgcccgagga gtgaaacttc ggtcttgcta agtcccacgc
    17041 ttcggtcagc aagcatatct tcactcccaa cgattttgac tttgtaactg ctggttgaga
    17101 tagttcggta ggtttcggtt tgcggatcaa agtaagaaaa ccaaatcgct ggaacagtta
    17161 gtaatccagg ccgacgggga attagcacat attcataagt cactgagcct ttaatacttc
    17221 cgctcgactt gtctattttc ttttcgattt taggttcaaa aacctcgacg gaattgggta
    17281 gactgggcgg tggtaattta atagaattaa tattaccact accgctaatt ttatatcgaa
    17341 gtgtcagagc ttgatcctgt tttacctgca gagtatcaat tttgctttcg attttaaatt
    17401 gcccaactgc tccaataaat gaagcgggct ggccgctttc gggaagcggt ctgactcgaa
    17461 tcttaatcgg ctccgattga actgttacaa tctttgtttt cgaaaacatt ggatcatcga
    17521 aaaaagaacc gccaaaaaag tcgtcgaaaa tcgaacgccg ttgactaggc tggattactt
    17581 cacaatcaac taccattgga tcaatggtca attccccggt agtagttgcg aaaagggcta
    17641 tttgctgaat tgtcgcctta cggtaggcaa caccattgac ggtttcatta gttataaccg
    17701 gttgagtagg cgcgtccagt tcttccttcc agaatccctg agcatcgggt agtttcttac
    17761 ggccgaaggt gcgcacattc ttaaagtaca gatcaaatga aactattaat tcttcaccca
    17821 tatatatcgt agttttaggg acaacggctt ttaggaaaac ttcctcgccg ctactcggag
    17881 tcacttgttt atcgggagtt cccgaaggtg gtgcggaacg gggttgggta ggcgcggaag
    17941 cagtgacgtt aacaatgacc ggttcagttg aatagatttt gccgcgatat ttgatcgaaa
    18001 taggattaat tgttagtttg ccggcacgag taggtgcaat ttgccagctg acggtttttg
    18061 aggcagtcat cttaccattg atgatttgaa tactgctgga ctgcgatggt ccagaaatca
    18121 ggacaaagtc tggactttca gggggtggaa cttccggaaa atcactaatt ccttccaacg
    18181 taaccgagaa aattagcaca tctttgtcgg taatattgtt ccggttgacc gaggcggata
    18241 tgcgcaagtc ttgacctagg gcgtgcagac aaattaactg caagaaaaac agtaagttga
    18301 aggtttttaa tctattcatc caggaattac caatcttttt ccttagccag accagaagcc
    18361 ttcattcgca ttttttcctg cattagtttt ttctcatcat tcttcaaggc atccagaatt
    18421 tgcgcggctt ccttttgact tttttctttc tcttgccgat tttgctgaga tggttgttgt
    18481 tgagcctgtt gctcttgttg ctctttattt tgcggctgag attgttgatc cggttgaggt
    18541 tgttcttctg gctgattttg ttgatccggc ttttgctccg actgattttt atcttgattt
    18601 ttctgttgtt gcaatatttg ccgagccaat tcataattat atttagcatc gaaatcgcgg
    18661 ggattgtatt cgagggcttt tttataaaaa gccagacttt cttcaatacg gccactctgg
    18721 aataaggtat ttccgagatt ataatagact gccgattttc gcattggatc tttgacggaa
    18781 atagcctttt caaattcacg cctagcttct tcgatacgct ggcttttata aagggcggcg
    18841 cctttgccaa aatgggcttc ttcccaatcg tcgtggtctt tcagaatacg gtcataagcg
    18901 cggattgctt cctgataatt gcctttttta tactgacgca gaccagaatc agccgccgat
    18961 attttcagcg gacataagat aaccactccc agggcaaata aacttaaccg acttatacaa
    19021 tttgaaatgg atttagatct cgtcatattt gttcaagaat tagtatcacg tgaggttgaa
    19081 tttctctttt ccggaattaa aatttcagta gtcaaaagca caagaccaat taataaaaat
    19141 atctgatagc gttgctgata atcagaatat tcatgcgatt gcagattctt cttatccata
    19201 ccagatattt cgtgggcaat tttctgaaag gcatctttat tggcactcag gttataatat
    19261 tttccgcccg tcatcgccgc aattttttgc atggtttcct cttcgagcgc agtcattacg
    19321 atatgtccgg cacggtcttt cttgaattct acttcaccag tcgtgatatt gcgcagaggt
    19381 atcggtgccc cggtgagcga accgattcca acggcataaa taactacacc ttcctcagcc
    19441 gcctgcttgg cgatttgatt aatgtctccc tcatgatctt ctccatccga aataaggata
    19501 atgactttgt gctttttttc gcccgattta aaggctttta aactagtact aattgcatca
    19561 gcgatggcgg ttccctgagt accaattatg ccagtatcac acgcatcgag aaataactta
    19621 gccgcactat aatccagagt cagaggacac tgcaggtaac tggtgccggc aaaggcaact
    19681 ataccgattc gatcgcccat caaaccatca ataaagcgat tgagctcaaa ttttgcgcgt
    19741 tccagacggt tgggtttgac atcttcagcc ttcatactga tggaagtatc gagcgcaata
    19801 ataatgtcct gtccgcgtcg tttgacctca gtcagtttct gaccgaccgc cggtcccagc
    19861 aacgcaattc ccaagaaaac cagtccgacg agccaaagat tattttttag acgccttaat
    19921 cccgggcgat agccttctaa caatttctga gaaagttcga ttccggcata ctgttggagg
    19981 ctcttccgat gctgaattcg gttataaata agcgccagta tcgccagaat tatcaggctc
    20041 cagaaccaga gaaattccga atgataaaac ttgaccatca gggtgttctc cgccagatgg
    20101 ttaatccgat caacccactt agcgcaacca ataaaaagcc cggcactaaa aagtaaggat
    20161 aaagttcgtt ataattgacg aattcgtgaa ccttgacttc ggttttttcc attccgctga
    20221 tttcctcata aatcatcgcc agactttttt cgtcagttgc ccgaaaatac ttaccgccag
    20281 tttgattagc aatatcctgc aaaagaactt cgtcaatttc agcatttacc aaacgatatt
    20341 gaatttgacc attgggcaat tcaatcggat aaggcgcttg ccctaatttt ccggctccaa
    20401 ttgtatagat tttgatatcg aaggcgtttg ccagattagc ggcggtgcgc gggtcaattt
    20461 caccggcatt attacagcca tcggacaaga gaatcattat tttgctttta gcctgactct
    20521 cacgcagacg attggtagca ttggcaattg ccatcccgat cgccgtccca tcgtattttt
    20581 cttcgacaat atcaacctga ttcagcaaat ccttcaacac atcataatca atagtcaagg
    20641 gacattgtaa aaaactctcc gccgcaaaaa cgaccaaacc aatgcggtca ctttgacgcc
    20701 cttcgataaa cttttgggca accgatttag cggcttctaa ccgattggga cggaaatcaa
    20761 tagccttcat cgagctggat atatccaata ccaacattat atcaattccc tccgtggtaa
    20821 attcccgcct ggtattgcca ctttgaggcc gagccaacgc cattattagg agagcaattc
    20881 cgattagttt ggcaacaaac ggaccatgca ctttataaaa ggcgtggcga tttttaacct
    20941 gtaaaatgtt tttaattgac gaataacgca gagcgccggc atttttgtgg cctcttttcc
    21001 aataccagat agccaagacc ggcacaagaa ttagtagaac taaaaaaccc caatttgcga
    21061 aatagaacat acagcttaat ctggtaggtt actcctcata atttcgctct atataatgag
    21121 caatccgtaa aattgtttct tcatcgaaat agcggccaat caattgggca ccaatcggta
    21181 atccttccga cgatttaccg agtggaaaat tcaaagccgg cacgcccgct aaattggctg
    21241 gtacggtata aatatcacca aggtacatag ccagcggatc gtcaattttg gaaccgattt
    21301 cgtaagcggt tgtcggtgta gttggcgtca gaatcgcatc tactttctta aaagcctcta
    21361 cgaaatcttc ctttattaaa cgcctgacgc gctgagcttt gtcgtaatat gcctcataat
    21421 atccagctga caaaacgaat gtaccaagca taatgcgacg agtaacttct gctccgaatc
    21481 ccgcatgtct ggtctcgcga tatagagtat cgagatcgcc accgcgttga cttaaaccat
    21541 atcggatacc gtcatagcgt gccagattgg aagaagcttc ggcggtagca atgatataat
    21601 aagtggcgat ggcataaggc gtatgcggca aagtaatatc ggtgacgatc gctcctgatt
    21661 tttccaaaaa ctgaatgcac tgcttgatgc ggttggcaat ttcgggatcg agtcccttgc
    21721 taaaatattc ggtcggcacg ccgatgcgca gattgcggac atccttaccc agcgcattct
    21781 gataatttgg cactggttca tcggcggtag tcgaatcatt ctcatcgcgt ccggctatca
    21841 cctgtaacag gcgtgcgcta tcggctacat tgcgcgaaaa agtcccaatc tgatccagag
    21901 acgaagcaaa agctaccaac ccatagcgcg acactcggcc ataagttggt ttaagaccaa
    21961 taacgccaca gaaagaagcc ggttggcgaa tcgaaccgcc cgtgtcagaa ccgagagcta
    22021 tatccaccag gttcccagct accgccgcgg ctgaaccacc actggaccca cccggaactc
    22081 gcttctcatt atgcggatta ttcaccaagc cgaaagccga gaattcggtt gtagaaccca
    22141 tcgcgaattc gtccatattc attttaccga caatcaatcc atccgcctgt cgaattttag
    22201 taacgaccgt ggcttcataa ggcggcataa aattagccag catccgcgaa gcacaggtcg
    22261 ttggtaaccc agacacacag atgttatctt taattccgat ggtatatccg gctaatgagc
    22321 cggcttggcc attttgaatc ttataattta tttcatcata tttttcggca atatccggcg
    22381 caactgagat aaaagcattg tacggattag gcttaatccg ctcgagtaaa tgctcgattc
    22441 ctaatagaaa aactgtacca gccacccgtt ttattccttg ggtgtcggtt gttcagtcgg
    22501 attggttgaa tttcccgaat tatttttcgg tttggaatct acatccttta tttcatcttg
    22561 aatatcgctg gcggcttttt tgaattcgcg taaactcttg ccgatacttt tggccaattc
    22621 aggtaagcgt ttagcgccaa acaaaagcaa gaaaacaatt aaaataagaa ttatttcgcc
    22681 agttcctatg tttcccattt tgactccttt agttatctcc aaaatctcag gatataatag
    22741 gctacagcca gcccaattat cccgataaaa tttaatttca aggtaaagcc caacgtaatt
    22801 gaaaacatta tgagatcaat tacagctggt ccaagcataa tatcaattga ccgtagaaaa
    22861 acttctttga ctactccatc gggaattaac gagcgcagta attcaccgag cagagaaccg
    22921 atcattgcac ccagtaatag aataccaatc acgaataaaa tcgaaagttt gcgttttgtc
    22981 gaatccatct taatctttcc ctagcaaaga tttaaaaatc cccaatccgt ccactgaacc
    23041 gagcacctgt tccgccgccc gttctggatg cggcatcatt cccagtacgt tcttatcttc
    23101 attcagaatg ccagcgatgt taagcttcga tccattggga ttggttgccg gattaacttc
    23161 cccatcagaa tcgcaatact ggaaaacaat tcttccctgg tcttgaagtt gcaccaaagt
    23221 atcatcatcg gcaaaatact gaccacagta gtgggcaata ggcattttta aaacttgccc
    23281 ttcactatac tcgctggtaa aagccgtggc tttattgatt actttaaggt aaacctcctg
    23341 acagcgaaat cgcatatgat cgttaggaat taaagccccg ggcaatagcc cggcttccaa
    23401 cagtacctga aaaccgttac atataccgat gacttttccc cctctggcgg caaattgaat
    23461 aatggctggc ataatcgctg aaaaacgggc tatcgctccc gcccgcaaat aatccccgta
    23521 agaaaaacca cccggtaaga tgatcgcatc gaaaagtcgc aaggtagtat ctttatgcca
    23581 gatatactca actctcgctc ccagaacatg tttaataacg tgataagcgt catgatcgca
    23641 attcgagccg gggaacacca ctactccgaa ttttatcatt ccgtctccac tatttcgtaa
    23701 ttatatctct gaatcactgg attagaaaga acgcggtcgc atatttcccg aaccaatttt
    23761 tcgacatcgc ttcgctcaag attggaattc agctttatct caaaatattt accgactgac
    23821 aattcttcga tattctgata tccgagagtt tttagagcct tttgaatcgt tttagcctcc
    23881 ggatcgagaa taccctcttt gtaagacact accactttcg ctaaccacat ttaatgcgcc
    23941 tttcttctgg ctaaatgaat acggggttca tcatagccaa ttaaccattg caacagatga
    24001 gtaattatat aaaccatcac caacggaaat ataacatatc cattgaaaat taccatcccg
    24061 attgtcataa gaattatcat cccggtttta aacgcagttg gcaagccgcg acgaaaacca
    24121 agtttgagat taacgctaaa gcgaatatta cttaccatca taaacgataa tactaccacc
    24181 agtggaagga ttatcttcgg attaccataa ttgcctaata ctttataact aaaccagata
    24241 taagagccta tcgtcagagc gctggtcggg gttggcattc caatgaaata gcttttcttg
    24301 cccgcaccag cctgcagatt aaagcgcgcc agccggacgc cgccaaataa aagcggaaaa
    24361 aagctgatta atccgccaat aaatcctaac tcggatacaa atatttcatg gattaacacc
    24421 gctggcacca aacaaaaaga aaccatatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24541 nnnnnnnnnc aaatgttaat tttcagacct ttaggaataa ttaattctac ccgcgaccca
    24601 aacataatca aaccaaatcg ctcgcctttc tctaaattct ggctgacttt cacccgcgaa
    24661 ataatccgcc gcgcacaaaa accggctatc tgagtcacag caagtttgag gttatctttc
    24721 tccatcgtta tccggcattg ttcgttctca atcgccgctt ccggcttaaa agccgagtga
    24781 aatttaccac gcttatgttc aatttgcctt ataatccctg aatagggtgc gcggttaata
    24841 tgcacgtcaa aaggcgacat aaaaatggca attttaatcc cgccgccgac aaaagggtca
    24901 tttacttcgc tgattcctac gacttttcca tccgcaggtg caattagaaa tgctggattc
    24961 tgaggagtga ttcgctccgg atcgcggaaa aaatagagcg taaagattat actcactaat
    25021 gtcacccaga aaagcaccat cataatcccc aaatcaaaat atttagccaa aaaaatcgcc
    25081 agaacagcca caatcaacat cggtataatc aggcgcaagc tttctttggc aatcattttt
    25141 gaatccctgt aatccgcttt aacatctcgg tataggcttc ctccacctcg cccaaatcaa
    25201 accgaaagcg gtctttgtct agtttccggt tagtgccttt ttcccagaag cggcaagtat
    25261 ccggtgaaat ttcgtcagct agtagaatct ggtcattata acgaccaaat tccagcttaa
    25321 aatccaccag gattatcccg attgaatcaa aatattcttt cagaatttga ttgattcgca
    25381 gagcggtggc tttaatagcc gataattcat cggcattagc cagattaaaa cggatgatat
    25441 ggtcgtcatt cattgccgga tcatgaaagg catcgttttt ataataacat tctacaattg
    25501 gctcactgaa aacctgccct tccggtactc cataacgttt acaaagactg ccagccgcca
    25561 cgttacgtac tactaattcg acggggataa ttttaacccg ccgtactaac atctcgttat
    25621 ccgagagttt tttgataaaa tgagtagcaa ttccgttatt ttctaaaagg ctaaatagct
    25681 ggcaactgat tagattgttc gctactcctt taccgctaat ttgccctttt ttaatgccat
    25741 caaaggcagt ggcgtcatct ttaaaaaact gaatgactaa ctccggatca tcagtagaat
    25801 agacgatctt ggctttccct tcgtataatt tttcattctt tatcataatt accttcgctg
    25861 atccgttcaa aaattctatt gattcgagtt aaaacttttt cgtaattaaa ataagactca
    25921 atttcggctg gatttaacca tttgagaaca ttctcatctt tcaaaagcaa ttccttaaaa
    25981 tcggagcccg attgccaaac cttcatcgca ttattctgga caatagtata agcctcttca
    26041 cgacttgcac ctttttcaat tattgccagc ataatctctt gtgaaaagat taatccgtgc
    26101 attccttcga gattacggcg catattttca gggtaaacaa ggagatttgt tagcacttta
    26161 ctcatttcat tgagaatgaa atcagtacaa atacaggcgt ctggcaggat gactcgctcg
    26221 ttggaggaat gtgagatatc gcgttcatgc cagagcggaa tattttccat agcggtcacg
    26281 gcatagccgc gcaggagtcg agccattcca caaattcgct cggaaagcac cggattacgc
    26341 ttgtgcggca tagcagatga acccttctgc ccttctgaga aaaattcctc aacctccaga
    26401 acctctgtgc gttgtaagtg cctgatttcg gtcgcaattt tttccagagt cgttccgatc
    26461 aatgccagag tagcaatgaa aaaggcatgg cggtcacgct ggataatctg gttagtgact
    26521 ggagcgggtt ccagcccgag attgtggcag acgcgttctt caacttccgg ctcaatgtgc
    26581 tggtaggtac cgacggctcc ggaaattttc ccaactcgaa tatcatccag tgccagctca
    26641 aatcgcttaa tatggcgcct gatttcctcg cgaaagatta ttaatttaaa cccaaaagta
    26701 atcggctcag catggacacc gtgagtacgc ccgatcatta aacaatcttt gtactcaata
    26761 cttttttcag ttaaaatttt ctccaactgg ttcaggtcag cgagaatcaa ttggcctgct
    26821 tcgcttaatt gaagcgccag agcagtatcg agtaaatctg aagaagtcat ccctttgtga
    26881 atgaagcggg cggctgggcc aacattttca ctgatattag tcaaaaaagc gataacatcg
    26941 tgtttgacct cttcctcaat ttcttgaatg cgttttaatg aaaattttgc ccgcgattca
    27001 atttcctgca aggcttcgtc ggggataatc ccccgctcat tttgaacccg acaaacttcc
    27061 agctcaactc tccaccaggt cgccaattta ttctcttccg accagattcg tcccattgca
    27121 ggtcgcgtat agcgttctat cattggctat tctaaaacta gctgggtttc atattcacga
    27181 ttaccacgta aaaatcggac tttaatctta tcgccttgcc ttagcaaatt ttcctggatg
    27241 taagcatata tttcttccgc cctggcaacc tttttcccgt taatcgatat aataacgtcg
    27301 ccgactttca gcccagagcg ttcggctgga gtaccttttt ccactcgtgt gacaattaca
    27361 ccggtattcg acggtaaatt tagatagcgc gctaaaaaac tatccacatt ctgaagctcc
    27421 cagccaattt taaaagttcg gctgtcctta ccgtactgct taagattagc tacaaccgcc
    27481 tttacccggt caaccggaat agcaaagccg attccgatcg agccctcact aaattgacca
    27541 ccggtgaaaa taaatgtgtt gatgccaatg acttcgccca gcgcatttac taatggtcca
    27601 ccactgttgc cggtattaat cgccgcatcg gtttgaatca tgtcctggta taggcgaccg
    27661 ctctgttgag gcccaaaatc gaggtcggtt cccgaaacta tcccgatcgt agctgtcggc
    27721 ttataattaa cgctaaaaag gccaaaagga ttaccgagtg caattaccca ttcgccaata
    27781 attacgtctt ccgaagaacc cagtttggca tacgtaaaag acttgccatc aatcttaagc
    27841 aaagcgatat cggaaatatt atcaatcccg actaatttag ccttatattc tttgccaccc
    27901 atcaaagtca caacaatctc ttcggcacct tcgactacat gagtattggt aacgatataa
    27961 ccatcagaac tgattactac gcctgaaccg atgcttttta cccggcgacg atatatctgc
    28021 tcggggaaca gcaaactcca gagcgggtca ttgaaaaacg gagaggctac atactcctca
    28081 atcgccgtga cgttaatacc cgccacagcc gggctgactt tttcgatggc cgcagttatg
    28141 gcattgcggc gtgaattact gatttcttgt tgttcttgtg gaacagccag taaaaggctt
    28201 acgccgataa acaaactgct aattaacacc gaatttattt ttttcacttt tcgacctttt
    28261 tccaatcctc cagaaattgg gtgatgccct tatctgttag aggatgtttg accatttttt
    28321 ccataacatt gaaaggaatt gtacaaatat ccgccccgat tagagccgct tccacaatat
    28381 gcaacggatt gcgaatactg gctacaatga tttccgtcct gataccataa ttgtcataaa
    28441 tagtcttaat ctgctgaact aattccatcc cgacctgatg aatatcatcc agacgcccga
    28501 cgaagggact gataaaagtt gccccggctt tcgccgccag aattgcctgc aaaggcgaaa
    28561 aaatgagggt cgtattagta cgaattccct tctcggataa aattttgata gcctttaatc
    28621 cttccaaagt aatcggaacc ttgatgacca tattcttatg tagagcggct aaatcctcag
    28681 cttcgcgcac catgccttca gcatctaaac tgacaacttc gccacttacc ggtccattga
    28741 cggtctcgca tattttgaga agaatctctt taaaattgcc cttttccttg gataacaaag
    28801 tcggattagt ggtaacgccg tccaataatc cccaggactg agcttttcta atttcttcga
    28861 gattagcagt gtcaataaac agtttcattt ttaattgtcc tcctgatatt taacattcca
    28921 taaaaacagc ccctgcgccg gagctgtata aacttgcatt ttagtatcat tttttccaag
    28981 aagattcttg aaagcaccga tcgaatattt tcctctggca acttccatca ttgaacccac
    29041 cagcatcctg accattgcat ataaaaagcg attggcggtt atgatataaa ctaatagttc
    29101 gtcctgttct tcccagcgcg attcgtaaat cgtcgccatt ttattttccg tctcggtcgc
    29161 cgctcgacat agcgccgtga aattgtgttc gccttttacc aattcggcac actcctgcaa
    29221 caactttcga tcaaagcgaa aatttaccag ccagacatat ttgcgtaaaa atacgttagg
    29281 gttgacaacc acctggtagt gataggtacg attaatcgcg gtaaaacgag cgtgaaattc
    29341 cttccctacc tcctggcaat agtctatgta gatatcatcc ggcaaagttg cattcaaggc
    29401 ttttctgata gtttctggtg ataaatccgt caccaaatca aaatgggcgg tctgacgcct
    29461 ggcatgcaca ccgctatccg tacgaccagc ggcaatcacc gtcaccggct gattctgatt
    29521 cagaggttgc agggcatttt caattttcgc ctgcacggaa ggctgataac gttgacgttg
    29581 ccagccacaa taattgcttc cgtcatactg cataataatt ttaaagcgac gtcccatcag
    29641 atattaataa ccatcagaac caagctccca caaccagcca ataatcctat ccaatcaggt
    29701 gtttgccaat gtaatcggcc gatgtaggag cgcttaactt gagaattata accgcgtacc
    29761 gtcattgcct cggctaatac ttccgcccgt cgaaagctaa ccaccattag cggaaccaga
    29821 atcgttcgca ggcgctcaat ttgaccaaat agaccacctg aatcgcggtt cataaaaata
    29881 cgccagctat cgtcgagcct ttgatactct tcttgaatta gtggaataaa gcgcactgcg
    29941 ataaaaatta tctgaaaaat atcatctact ggcaaaccga tttttctcaa gggctttagt
    30001 aaatggtata gagcatccag tacctccagc gcctttaacc aatgagtcgc gagagccatc
    30061 agagctatca aattaccgat cctcaatgta aaggtcagcg gcgtaaatag taaaaccgac
    30121 caggaatcac gaggagctga acggaaaata gtggctagca agtttgttcc aaacaaaata
    30181 tgaatcaaaa atgtcaacgg taagaatacc agcaaaattc tgaggacctt gaaattttcc
    30241 tttgcataga ggattctaaa agttatgata gttcccagta tgatagcgac tccaagctgg
    30301 gcataccaaa ctttgaggcg gaaaatacct attacggcta ttatcagcca gagaattttt
    30361 acccgaggat caaggttttt cattttagaa tttaagactc agcataaaag acatgccatc
    30421 ctgatatggt tgccagttgg attcaatatc tatatccggc atttccaatc ggttgcgcgt
    30481 cagaactatt acatccagca tgctaaagag tcggttggct accaagccgc cgatagcaaa
    30541 agtcgcattc cgtaaagctg tagcgctttt cactcgtagt ttgtcaaagt gacggcgaga
    30601 agattcactg tcccacgccc agtaatattc gggagtcacc ggatagagca aattaattgc
    30661 ccggtagcgc aatttctgtt cattataggc gtagatattt tcataattgc ctatgtcggt
    30721 gaaataatat tgatctttgc cgtctggatt cactccggca tgcgtggcgg cataggcttc
    30781 catatcagcg gcaaccgcat ttccatacca ataaaaagca aaataagtca cccagagagc
    30841 tatttcggag gcattaaaat aatgactacg ttcaacgtaa ttcaaactgc gttcacccca
    30901 gccgggaaga acagccgatt ttaataatag cagacgcgcc tgactttgag tcaattgggg
    30961 aagtgaatca ggttgagcga caagcgtaga atcaatctga gctgtaccgc ttcctgcaag
    31021 aataaaaatt attaataatc ccaagagact cgggcaaaat aaatgcttca ttttttcctt
    31081 cttattattg aatagccgat taacaacaat gccagtataa cacataagcg cgctggccag
    31141 ttcccatatc tcacaaaaat agtttgtgga tttccctgag taactggcgc tgaaagccac
    31201 cccgatttgc caaaaggtaa ggattgcaga ttcctgccat attggtctaa aataagtgaa
    31261 attccggtat tggcagaacg gacaaccgga atacgattct cgatagcccg ccaacgccca
    31321 atctcagcat ggagaaaggg tgccgaagta ttcccaaacc aggcatcgtt ggaaacaatc
    31381 accagcaatt ccgagccctg gcgggtagcc tggcgaacta gatctgggaa agttgattcg
    31441 taacaaatca cggaattaaa agtcaaaacc gtatcggaga ccggcctttg gtcaacttta
    31501 aggggaatct ggaataactt aacctctttc ccagcctgaa aattgccttg cccgagatta
    31561 agattactca attccggaaa atagccagaa agcgggatat attcgccaaa cgggacaaga
    31621 tgaattttgc gataatcttc aatccggttg gaatgcggac ggagcagaaa agtcgcattg
    31681 taataccgat aatcttctcc cgcacgttcc agatccggag taccggttaa tagattaact
    31741 tccagtcgtt cgatatgctc tcttatttca gtcaaaacgg cataatgatt ccgtcgcaaa
    31801 aaagtcggcg tagcagtttc aggccaaacc accaggtcca gcggctgggt cgccaccgaa
    31861 tcggttaaat cgtgcaacaa ttgcatattt tttttgaaag attcctgatc ccatttggcg
    31921 ttgggatcaa tattaggttg aatcaagccg acattgacta aacgccggtc gttttcgtct
    31981 gatggttttt gcaagacgac cattccatag attgctggaa ttaaaaatag ggcgataatt
    32041 atcaccatgc gccgactcac ctgacggtaa tcttcgagat tttccagcaa gcgaaaaata
    32101 aggacattaa tgaccactat ccagcccgaa attccatagg gaccaaccac agccgccgat
    32161 tggattaact gaatggcatg cgactgagtg taacatagat tattccaggt aaaacctaat
    32221 gagccaaagg aacgcagata ttcgatagca gtccagataa tcggaaatga ccagatgccg
    32281 acgttccgtc ccagtcgccg gccgataaag ccgacgctca aaccgatgat agcataattc
    32341 atagccagaa aaataacagc cgctaccata gataaagtcg cccagtacca ggtagttcct
    32401 ttatttaacg ccaaccagta cataatcgcc aaattcagca ccagcccata aaccaatcct
    32461 ttttcaaaac cattatgaaa ttcatcatca caaaaagctt taatcaaagg aaccaaggcg
    32521 aaaaaagcca aaaatcccag cggcaaaggc ggaaaagcca atccacttaa tacaccagaa
    32581 attaatatca accaccaaag gttgattttg actttcatca tctttggctc agagtttttt
    32641 ataaaggtga agtacttttt cggcgtcttt tacgctttct tcatcctttc tttcagtagc
    32701 cgaccgggtt tgaaatgagt tttgcggcga gcaggaacat atacttcttc gttagtgcgc
    32761 ggattgcgag ctttgggttt ggctttagtc ggtttgactt caaaaacacc aaaattacga
    32821 atttcaatcc ggattctcgg gtcagattca ctgagcattt cgcgcagaac cataaagaca
    32881 ccgtccacaa tctcggcagt gtcaaccaat ttggtatcaa gcgcttcagc tgtgcgtctg
    32941 acgacatctt ttttggtgta agtttttact ttcattttct aatctccttt tgtttattta
    33001 cgttctactg aaattatacc ctgcacattt tgcaatttcg ctaaaatttt attgagatga
    33061 cgcagatttt cgacctggat aaccagattc aaatgaataa ttgattcatc aacgtcacca
    33121 ctcacgctaa taatattcgt atcggtactt gccattatat cggtaacatc cttcagaaat
    33181 ttcttgcgtt cctgagccat tattttcaat ctgactaaaa aggcttgcgt tcccgcgata
    33241 tcccattcta cgttcaatag acgttcatca tcttctaata gagaaggaat attcgaacag
    33301 tcactccgat ggattatcac tccccgtccg cgactgacaa agccgatgat ctggtcaccc
    33361 ggaataggat tacaacattt ggcaaacgaa accatcaagt tacttatacc ttgcaccttg
    33421 atgcctttga ccgatttacg agccgtttcg ataaaaagtt catcgaggtc ctgacgaacc
    33481 agctgttctt ccttctgagg aaagagtttg gcataaatct gggggatagt tagaatacca
    33541 ctcccgaccg ccgcatacag tttatccagc tcgctgaatc cgagttcttc gaacgattcc
    33601 tgcacggttt tcaaaaatcg caattcttta ttgcgctggt tttctttctc cagaatttcc
    33661 ttcccgagat tgatactttc ttcgaattga gctttgcgta aataacgctt gattgctcca
    33721 attgctttgg cagtcgtgac aaatttcagc caggcataac tgggtcggtg agaatcggaa
    33781 gtgatgattt caatggtttg gccactgcgc aattcgctat ttagcggtac catcttgccg
    33841 tcaactttag ccccgataca gcgtgttcca atttcagtat ggacttcata ggcaaaatcc
    33901 accggagtgg cccccttggg taagcgaatc agatcgccca tcggcgtgaa gacgaaaatc
    33961 tcatctttaa aaagatcaat cttaagggta tccataaagt ctttggcgcc ggtagattcg
    34021 gtctgcagga tctcgattaa atcgcgtaac catttgacat atttatcaat gtcgtccggt
    34081 ttgtcgcgac cttccttata acgccaatga gcggcgatac cctcttcggc gatctcatcc
    34141 atttcggttg tccggatttg aatttccacc atacgaccac ccggtccgta aaccgtagtg
    34201 tgaatggatt ggtagaagtt ggctttgtgg ttggcgatat aatccttgaa ccggtccata
    34261 accggcgtaa aaagatcgtg aacaattccc agcaccgtat agcaattttc tttagtatca
    34321 actattatcc ggactgcaag caggtcaaag atttcctcaa aaggtttatt ttgagttttc
    34381 atcttcttat aaatcgaata aaaattcttc gggcgaccga caatcttggc tttaatgccc
    34441 tgttcgttca aagccttaat aattggctgg gtgaagactt taatatattt ttcgcgttca
    34501 ctgcgttttt cgttaacctt tttttcgaga aatcggtaag catccggatc cagggctttc
    34561 agaactaggt cttccatttc ggattttaac ttgaacatcc ccagacgatg ggcaagcgga
    34621 gcatatacat cgcgagtttc gatcgcaacc cgacgttgtt taatcggtgg cagagaatcg
    34681 agcgtttgca tattatgcag acgatcagcc aatttgatga ttagcacccg gatgtcttca
    34741 gcaacgctca aaagcatttt gcgaaaatta tcagcctgtt cttcttgccg attgcgaaag
    34801 gtaattccgc taattttggt aacaccctcg actaatttgg cgacatcgtc cccaaatttt
    34861 tcggcaatat catttaccgt aacattagta tcttcgatga catcgtgtaa taatccaccg
    34921 gcaaccgtta tcgggtccat atgcagatcc gccaaaatca aagcagtgtg ataaggatgt
    34981 tcaaaaaaac tgtccccgga ttgacgacgt tgaccgcggt gcgagttacg tgcaaaacta
    35041 tatacttccc aaagaaaatc ctcctcatct ttagttaaag gcgcgcgctg attcaactta
    35101 ctgatcagag taaggaattt ctggggacgc tgtttcgcgc gcggtaggac tttattgagt
    35161 atttcagttg ccattaaaat ggagtcgtta tgatttcttc tctttgggca cccttatttt
    35221 caaaacgagt atatttatca ataaagataa cttcgacggt gtcagtaggg ccattgcgtt
    35281 gtttagcgac tatgatttcg gcgagacgtt catcttcgtc cgatgcggca ctaccttcac
    35341 gcttatcgta aaacgatttg cgataaatga agatcacaac atcggcatcc tgttccagcg
    35401 aaccgctttc ccgcagatcg gatagttgcg gtcggtgatc ggcgcggcct tcgaccgctc
    35461 gtgataattg cgaaagagcg atgactggta caccgatttc tttagataag gctttcagtc
    35521 cacgtgaaat ctcggtgatt tcctgctggc gattttccgc ccgatggccc ttaatgagtt
    35581 gcagataatc tataatcacc agctggatgt cgtgctcggc tttggcacgt cgggctttgg
    35641 cgcggatatc catcattgtc agcaccggcg aatcatcaat atagatttta gcgttggcta
    35701 aacgcccagt ctgcatactt agcctttgcc attgctcctt cggcaattta cctttacgta
    35761 ctaaatggct atccagcctt gcttcggcac ataggattcg taaagccagt tggtaatcag
    35821 ccatttcaag actaaagaaa gcgactggca tatcatattc tacggcggca ttgcgtgcaa
    35881 ttgtcaaagc caaagcggtt ttgcccatac ccggtcggcc agccagaatt atcaattctc
    35941 ccggatgaaa gccggaggtc aattcatcta aatccaagag tccagtcggc acgccactcg
    36001 tatgcccttt ccgactgtga atcgaatcaa tgtgttcaaa tgtgcgattg agaacgtcca
    36061 cgaaattttt aaagccgcct ttcatccggt tttcggaaat cgcaaatatc ttctgttcgg
    36121 cggtatctaa aatatcctgc accgaatcgc gcgcttcata agattcggtt tggatttcat
    36181 tagaagccac gattaggcga cgtaaaattg ccatatcgag gacgatattg gcataatatt
    36241 caacattagc ggctgaagga gtagcctcaa ccagtccggt gacataataa gcgccaccga
    36301 cctgctccag catttttttg cgtttgagct ggtctataac gctaacctga tcaaccggct
    36361 gtccttcatt aaagaggtca gtcatagctt gaaaaatatg ctggtgagct tctttataaa
    36421 aagcggtata atcgaggtac tggagagctt tactaaccgc ctctttatca agcatcatcg
    36481 ccccgagcac ggaaacttcg gcttcgtcgg aatgcggagg gactctgatt atttcaccgt
    36541 catttaaagc catttattct cctgttttat ccaagtataa gttcttgatt ttcttgaaat
    36601 cttcccaggc atattttttc aaattctcat tgcgcaaaag ggctgccgga tggtaagtca
    36661 cgattaaatc cacattatga tactgaaaaa tctgaccgcg cattttctcc agagtatatt
    36721 cgactcgtaa taaagtttta gctgaggtca aaccgagaca tattatcaat tcaggctgaa
    36781 ttattgcgat ttgtttcaaa agataaggct cacacttttc aacctcgccg acattggggg
    36841 tacgattttc tggtggacgg catttcaaaa cattggcaat ataaacctga gcgcgggtca
    36901 agccaatcgc ttttaacatt ttatcgagca aattaccagc tcttcccaca aacggtaatc
    36961 cttgcaaatc ttcattaact cccggtgctt caccaataaa tagcagttta gcatgaggat
    37021 taccggttcc aaaaaccacc ttagtgcgag tttcggatag atgacattgt cggcagtttt
    37081 gacaaatgcg ctccaattcc gctagctggg tggcggcgtc agttttcggt gtagattcct
    37141 cgccattttc cgaaattaaa tagatttcgg ggggaaataa ttcagcctgt tgtttgaaaa
    37201 atgttttgat atcgtcgtat agtctggtca tttaagtttt cttataattt ttaataacag
    37261 tcgaaatgat tgaatcggcg atgtcacgtt tactggctaa aggaaaaacc cattcttgac
    37321 catcttttga taataaagta acactattgg tttcatgccc gaaagccgca ccggtcacgt
    37381 tgggatcatt ccagacgata aaatccatct ctttgtcgtt cagtttggct aatgaacgcg
    37441 ccttggcacc aggatgctcg gcggattcaa tctcgacgct gaatccaacc acgcatgcct
    37501 gacgattatg tttgcgaaaa tccatgataa tatcaggcga atatttaagc tttagctcac
    37561 taatgccact ctctttttta atcttctgag tggcaatttg cacgggtgat aaatcttcga
    37621 cagccgccgc cataaataaa aaatcagctt cagccgcttc agttaggacg gcggaacgca
    37681 tttcgttcgc agtctcaact cgaattgtct ttacgcccac cgtatttttt aaatcagaag
    37741 gtccactaat tagaagtacg tcggcgcttt cgcggaatgc aatttccgct agggcatagc
    37801 ccattttgcc actcgaccga ttggagatga accgaatcgg atcaatatat tcgcgagttg
    37861 gtccagccgt cacgataact tttttatctt tcagccattt attgccatta aggcggcgat
    37921 caatatgcgt cagaattgtc gtctcgtcag ccaggcgtcc gcgcccggtg ctaccacagg
    37981 ctaaggcgcc ttcctccggt tcgatgatgt cgtaaccgag tcgccgcaga cggctgatat
    38041 tctcctgagt aatcggatta gcatacatat tatcgttcat cgccagagca aagaccgttt
    38101 tgttcccgcc aaccagaatg attgtactga gcaaatcatc agcgatacca ttagcagctt
    38161 tagcgacaat attggcggta gccggagaca ccagaatcaa atcggcttca gtcgccagat
    38221 caatatggcg agtctcaatt ttctgaccgg taaacatatc ggttaaaacg ggtttgtggc
    38281 agaaggtttc aaaaatcagc gggctcataa acttttgggc ggcgtcggtc ataatcacgg
    38341 taacatccgc cttatagtca ttgaccagac ggcgcaaaag cccagccgat ttataaatgg
    38401 aaatgccgcc ggttaagccg agaacaattt ttctattcac taaattcact atcgaattcc
    38461 tcaagtggtt tttctttttc gggggtcata tagcgatagt tgattttgcc ttctaaaatt
    38521 tcgttgatgg ctaacgtggt cggcttttcg agatagtccc aagccggttc gtcggtgatt
    38581 tccggtggag tttcttcgaa agtctcctct tgctcctcaa ccatcgtcgg caagggatat
    38641 tgagccatac gcaaggcatt aatctgacgc gcgcgcttgg cggcaacgac caccagttcg
    38701 aaaatatcgt ctgatttccg agttagtttg ctaaaaggaa gggtttcagt cattttttaa
    38761 gatgctcctc tatgatatta ataattttat tgacggtggc ttccagattg tcattgacaa
    38821 tttgaaagtc gaactgttca cttagttcca attcatctgg gattcgggct ttccgcaaag
    38881 cgatggaact cgagtcatcc gtcccacgtt tctggagacg ccggtcgagt tctgtatcgc
    38941 tgggtggtaa aaggaagatg ctaattagct ggtcggggta attttttttc aaattgagtg
    39001 tgccttttac atcaaggtct aaaagtaaaa ttttgccatc tctcaaggct tgttcgattg
    39061 aatcttttaa agttccgtag taattgccga aaatctcctg ccattcagcc attttacctt
    39121 cgtgaatatt tttcagaaat tgctcacgac taatgaaatg ataatctacg ccgtcgcgtt
    39181 cattcggacg gggcggacga gtagtacaag aaaccgaaaa aacaaaacgg tcatcccttt
    39241 tcattagttc atggcaaact gtggtcttac ctgttcccga tggagcggca atagctacta
    39301 aaagtccttt gggtttcata ttacgttctg gatttgttca cgaatttttt caagctcatt
    39361 tttgagactg acgaccagat gagaaattgc ggtactttct gattttgacc caatggtagt
    39421 aatctctcga ttcatttcct gggctaaaaa attcatctgc ttgccgaccg gttcttcgct
    39481 ttgtaatagt aaggtaaatt gctgaatgtg actgcgcaga cgggtgcatt cctcagtaat
    39541 gtccaatttt ttggctaaaa ttccggcttc ttgaatcaag cgattttcat caagttgaca
    39601 attttggcac agttccgcca gactctgctg gtatttcgtg aaatattcct ggcgcgattg
    39661 ctgtgagagt ttttcaatct ccgaacattt ttccaagata aattgcaaat ggctatgtaa
    39721 ttctcgggta ataaattcgc cttcccggcg acgcatagct attagctcag ctaccgcctg
    39781 acgcagaagg tcttttacct gagttaaaag ttcagcaaca tcagtatccg cggaatcggt
    39841 tataaaaatt tccggatagg taagcaaatt ttgaagagta accggctctt cgatttgcaa
    39901 ttcgcgcgcc agatcacata gcgctcggta atatttggtc aaaaaagcag tgtttaattg
    39961 cacctcgggg gttttttctg aaccggtaat tgcgatagtg atgcggactc gaccgcgccg
    40021 cagatcgcca gctaccaagc gcttcaactc ctcagtgtat ggttcgagcg gttcgggaag
    40081 attgaaagtc aggtctagaa agcggttatt ttcggagtaa atctcaacgc tgattacttg
    40141 accgccgcta cggtactccg ttctactata acctgtcata ctttccaatt gcaaatctcc
    40201 tatcaaatca atggtaaaat tagttaatcc agtccaaact gtcaaattta aaatcgccat
    40261 ttaatcttta ttttatatta aattgcaacc tatttttatt tatgaagaaa ccaagtggaa
    40321 tttatcagcg cctcacgaac tatatagctg taaccggttg ccatccaaac aacatccgct
    40381 tggcgctttg ataaatgctc ttttagctct actttttaat atgtttaata gctggttaca
    40441 tattgaaaga ttggcacgcg aataccaaca attgatcggc ggtgcaattt gcggaatgcc
    40501 ctttacctat caaaaggatc atttgcatat tccgttggaa atggcgggag aatggcactg
    40561 cctgcatttt agcgttcaag cgcccctacc attttttact ttagaaagga ctttgccgca
    40621 accaaaacac aaggtcaatc tgcttcgtga cgtaaccgca aaagcaatta ccgatttggt
    40681 ttgccgtgaa aacgaccgcc aggttttaat tactttaaat agtgggatcg cctaccttct
    40741 attccatctc tatggaatca atggaaatgt atatcttctc gattcatccg gcgtgagccg
    40801 ggattcattt aaaaaagcaa aaaagccggt agactttgag ttgagcaatt tccgacctgc
    40861 caatcgcctt tttcccgatt atgccgattt taccagttca ttgcgtcagc attccgatca
    40921 gtcactgatg acttttttca ccaaatcatt tcaaccgatt ttaccgaaaa ttctcgtgac
    40981 cgaaatttgc tatcgaacta atctggcgcc gcagtattca attatcaatt tgacggacga
    41041 acaggttgaa agactcgcta aaactatagc cgaattatca gacgaaattg taacttcttt
    41101 accacggatt tatagcggcg agccgccgat ttttagttta atcgaactgc aatcgctttc
    41161 aaatcgcgaa ttcgccgagt acgactcagt tatcgccgct caaaaccatt ttattcagga
    41221 ttacctgaac ctctatcgct tgaaggaaaa gaaaaaaacc ttgttgagac aaaccgagtt
    41281 tctgctgagc actacccgga gaaaattgat caaacaacaa agcgaactgg cggctctacc
    41341 gacggcggat aattatcggg aatgggctga tacccttatg gcgacggcgt ccaccatacc
    41401 gcatcatagc aattcggtaa tcttgcctca attaaccgat gaagcccgcc agattaccat
    41461 tccgcttgaa ccaaaactca atccgattca aaatgccgaa cggtattacg ccaaatcacg
    41521 tcaaatcgaa aaagcaaggg ctgaattgat tcaaaaaatt gccgaagcgg aacagttaat
    41581 tcagcggctg gaaaataccc aaaaacaaat cgccgaggct gacgacttca aaactctacg
    41641 atctatatcc agccattggc cggctcagcc gcaaagctcg attgaaaata tccatcagcc
    41701 ctttattaat atcaatttgg acggctggga aattttaatc ggcaagagcg cccgtgataa
    41761 cgacgaactt ctccagaaat tcgctcgacc gaacgacttc tggcttcatg ctcagggcac
    41821 gagtggatcg catattgttg ttcgtaatcc cggcaaggtg gcgagcttgc cgcgtcctat
    41881 tctggaaaaa gccgccgggc tagcggcttt tcatagcaaa gccaaacatt cagcggtcgt
    41941 gccagtgatt tttacgcaat gtaagtacgt taccaaacca cggcactcgg cacccggcgc
    42001 agtaaccgtc aaattcgaga agacaattat ggcggaaccg ctcgacccta aaaaatttct
    42061 ctaaccac
//
LOCUS       DAOMJU010000008        40295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_815_length_40295_cov_19.447043, whole genome shotgun sequence.
ACCESSION   DAOMJU010000008 DAOMJU010000000
VERSION     DAOMJU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 40295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40295
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_815_length_40295_cov_19.447043"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..2107)
                     /locus_tag="PLO58_01535"
     CDS             complement(53..2107)
                     /locus_tag="PLO58_01535"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQO73502.1"
                     /translation="MGSFLRKISGLTIAPILLALSSNAFSAGLPTLRFEHLSTQDGLS
                     HNSIIEIIQDRQGFMWFGTLNGLDRYDGYNFTAYRPILGDTTSLCHNVINALYEDRRG
                     ILWIGTLDGLNRYDRARDCFIRIRHNPNNPNSLVDNRIRCIAEDNYGQIWIGTEGGLS
                     CYDPRVDTFQNYLPDPNNPNGLGDFIIHDIYNDSRDQLWIATEKEGVYRFNRITQSFT
                     RYRHETTESPKAGTSAVITIGEDRWGNLWIGTWGDGLQRFDVNAQRFRKVPIFCKGKK
                     PFTTKIITKLLIDQSDRMWMSTYDAGLFVIPQIPRNVTTAGIEGIQHYLCEPLDETSI
                     QSNALWTVYLDRTGVVWIGNENGGINKCDTKQSHILHYRFGLSGANSLSNNHVTSFFE
                     DQYGKIWIGTRFGGLNRFDPVTNHFRVYRKDEQNNGLSSDAILCLTGNRDYLWIGTDG
                     SGLDRYDFKSGQFKHFRHDDQNPSSLGENAVYALYLDKSNTLWVGTWGGGLSKLNSDG
                     QTFSQYPIDRINDRLNVVTSIAEDTSGGFWLGTYGKGLVFFNPNNEKMLFFRPDDKDS
                     LSLGHDNINTLFVSQDGTLWIGTMGGGLNYVQYFQADSGIAHFGRYDQRHGLPDNIIE
                     SLIEDGDGFIWMGTNHGISRLNPANGEIHNFDRNDGFGQDNFYRESVLRASNGMLYFG
                     GI"
     gene            complement(2171..3931)
                     /locus_tag="PLO58_01540"
     CDS             complement(2171..3931)
                     /locus_tag="PLO58_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQO73503.1"
                     /translation="MHRRMVTLILTLMLIVGCSEDKSAVDISIDSAEITGQTIVPPGP
                     EWLHNAVFYQIYPQTFYDSNGDGIGDLNGIIEKLDYVQSLGVDGFWINPFFASPFNDA
                     GYDISDYYQVAPRYGTNQDARRLFLEAEKRGLKVLFDYVISYTSIEHPWFQESARQEP
                     NKYSNWYVWTNNTWIDPPEAYRDAFIKGYSRRNGQFMRNFYWSQPALNFGFVKPESDW
                     MLPIDHPDVLALQEELQKVLRFWMDMGADGFRADMAGALVKNANIVESDPEYANRDEG
                     TKIFWQKIRKIIDEEYPQAFMVAEWSYPVAALDGGGFHADFFHWFSGYNDLLQKESWR
                     IMNGHSEGHSFFDREGKGDITQFLASYLEQYRQTVGKGYINLPLGNHDLSRLNIERSD
                     DDLEIIFAFSLTMPGIPFIYYGNEIGMRQLYDLPNVEGAYPPRAGARTPMQWSQGKNL
                     GFSTANPEKLYLPVDPAADAPNVADQQDDPNSLLNRVKNLIQLRKNSKALRAYAEFVP
                     VYAKKGEYPFIYARACEDETVLVILNPANKAATARFRLKPVFSQFELLAGTESELKLE
                     DKYYQLTIPGGSYSIYQLGN"
     gene            complement(4065..6026)
                     /locus_tag="PLO58_01545"
     CDS             complement(4065..6026)
                     /locus_tag="PLO58_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HQO73504.1"
                     /translation="MISAKIIRSFVTLLISLTMIQTCSSNKPKNQYLKSPDGNITVRF
                     EISPAGKIFYRVAYRNKEIVQASRMGIVREDADFSEGLKLDSVSASRPINFNYQMPQG
                     KKSHYAYNGNEKRFYLTNHVNQKIDVVFRASNDGVAFRYIFPGQSDEPKTILREISEF
                     HFSPRTKAFIQPMAMAKSGWCQTNPSYEEIYQQNVAIDKLPFNEPGWVFPALFEDGDS
                     WMLISETAPDRNYCGCRLQQDSTSHIFYIDFPSDLETFPGGIWLPTSPLPWSTPWRII
                     TIGKGLKNIVESTLGTDLAEPARLKEIDYVKPGRSSWSWVLLKDDSTVFEVQKRFIDY
                     AAEMNWEYCLIDADWDWKIGYDKVAELAAYAQTKGVGILLWYNSSGDWNSTTYSPKSK
                     LLTHSDRLREFARLKKMGIRGIKVDFFGGDGQSMMTYYQDIFEDVAQFGLVVNCHGAT
                     LPRGWQRTYPNLLTMEAVRGFEFITFDQADADLEPTHACLLPFTRNVFDPMDFTPVCF
                     SEIPNIRRITTNGFELATAVIFWSGIQHFAEIPAGMACVPDYIKNFMREVPVSWDETY
                     FIDGFPGKFVVLARKSGQTWYLAGINGADVERQLKLNCNFLDEDHTGQLITDASDGRS
                     FMTKEIHVSPQSPLSIKLEKHGGFIIKFD"
     gene            complement(6116..8536)
                     /locus_tag="PLO58_01550"
     CDS             complement(6116..8536)
                     /locus_tag="PLO58_01550"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HQO73505.1"
                     /translation="MPKILLMTISLGLFLACLPTCGQRIRLPERGSVSWLPGSKWEEA
                     LLTGNGTIGAMVIGRPHDETIILNHALLYLPASDRLPPINQASRLDEIRQLLLQGKYA
                     EAAKIPVEIGETEGYQGTHWTDPYMPALNIRLNMKAANVSDYARAVDFTTGEAMVFWL
                     DEGGRFERKLFVSRADNCIVMSIKGSGKINTSLEFAMHPVEWSQQEMVNGAIEFTEIG
                     AKDCWLTYRCKFKRHYDDNPQGYEAVGRVILKGGNLSSEGNQLVIQNADEILLLVRLE
                     PNFDWENSRVSELQSALQNQPTTYKTLLERHVNIHGNLYNRVKLDLGGRKLKTLQSEV
                     LVARAREEVTPPMIEKFFDAARYNILCATGTRPPNLQGIWNGTWSPPWSSDFTNDGNA
                     EVAISSLLCGNLPELMLAYFDYHEKLLPFYEDNARRLYGCRGIHIPSRTSSHGWDIHF
                     GETWCLTFWTAGAGWVSSFFYDYFLYTGDTTFLKERAYPFMKAAAQFYEDFLTIGENG
                     KYVFNPSYSPENNPANSNSQACINATMDVMVAKQLLRNCIQAAKILKVDESQAIKWQE
                     MLALMPEYEINEDGALREWLWPGLEDNYNHRHVSHLYGLYEMLDPDIAGNPELIKSAK
                     NAIAERMKIRRQENGGAMVFGLVQMALVAANLGDAEMAEELINWMASYYWSNSLASLH
                     DPGNLFNMDLSGGFPAAVIRTLVYSEPGMIKLLPALPNSWPAGSISGVLVRGQIEIKE
                     LKWRENQILVTLQSRVNQNLELRMPRPVGRILGPDRDLITRLPNDDRNFQIFLPVDSD
                     VTVKFVCQ"
     gene            complement(8615..>9237)
                     /locus_tag="PLO58_01555"
     CDS             complement(8615..>9237)
                     /locus_tag="PLO58_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HQO73506.1"
                     /translation="WNIFAVGSKQQVSYINGIHAVLVLGELIKDKYGLACRWNLANNW
                     NDGNDHGMFSQGGEPNVPRWNPRPVFYYMTYFKQFCGDHLVNTNVVGDNDVVAYASSF
                     SSGEAGIILVNKGNSPKSVRLEFANFQPGRRCYWYLLTGGKDNGDFSLQVFINGVSPE
                     LPAGGPADFEKIKAYSTKITRPLVFDLPPYSVQFLLVESECKIPVK"
     assembly_gap    9238..9337
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9338..10440)
                     /locus_tag="PLO58_01560"
     CDS             complement(<9338..10440)
                     /locus_tag="PLO58_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HQO73507.1"
                     /translation="MRRIWRSIIVLGFGIRILVGAEPPQGFFLYDFQPKKATIPPFRE
                     VTLTQSRSTVTVEIDIGDTLTKVPKYIYGHNANVYMTQMVNEPTLIGYIKQLAPQILR
                     YPGGNLSNLFFWNNQPGQLPEDVPDPLYGGVEKPYREEFWFGRNDTAITLSVDNYYKM
                     LEMTNCTGIICVNYSYARYGKGENPVATAAKYAADWVRYDNGRTKYWEIGNENFGNWQ
                     AGYKINTADNKDGQPEIITGELYGQHFKVFADSMRAAAHSIGAEIKLGASLLESAQDW
                     HTPTEKTWNSGFFKQAGDAADFFVIHSYYTPWNENSSVGTILNSAIPVTQEMMTFMQE
                     TARINKVKMKPVGLTEWNIFAVGSKQQVSYING"
     gene            complement(10577..10807)
                     /locus_tag="PLO58_01565"
     CDS             complement(10577..10807)
                     /locus_tag="PLO58_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73508.1"
                     /translation="MNNKKSIRICPNCGEYLDRIWFTAIMTEEWSWNGEGYSECTAHH
                     SLGTDPQEEVLCPYCEAVVGTGYDFGFGKGCN"
     gene            complement(11113..12246)
                     /gene="arsB"
                     /locus_tag="PLO58_01570"
     CDS             complement(11113..12246)
                     /gene="arsB"
                     /locus_tag="PLO58_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HQO73509.1"
                     /translation="MANERKLSVFEKYLTLWVLLCIGAGILLGKIAPGIAVKLDSLSI
                     YNVSIPIAICLFFMMYPIMVKIDFAEVVRAAKTPKPVALTLFINWAIKPFTMYLIASF
                     FLGHLFKGFLPGTEIIKTGQEVELWRSYISGAILLGIAPCTAMVLMWSYLAKGNDGLT
                     LVMVAINSLSMLVLYAPLGRFLLGVNAMPIPWQTILFSVMIYVALPLVAGYFSRKLIV
                     KSRGLEWFNTKFVHRLTPISMLALLITLVLLFSFKGEIIMENPLTIFWIAVPLFIQTI
                     FIFSLGYFVLARWLKLSYHDAAPAAMIGASNHFEVAIATATMLFGLSSGAALATVVGV
                     LIEVPVMLILVSICKRYCNLFGECHLDLPKCQPKYVTAEIKRA"
     gene            complement(12360..>13878)
                     /locus_tag="PLO58_01575"
     CDS             complement(12360..>13878)
                     /locus_tag="PLO58_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HQO73510.1"
                     /translation="KVISNEKGFKMGTLIFNRKKYPTLSIGIILLVFFRSVLGQDYIP
                     PDSTDMRNITSIELAQEMIPGWNVGNSLEAIGGETAWGNPQITQRLIDAVKAAGFNAI
                     RIPVAWSKFSDESTFTIEESWLERVEEVVCYVLSSGMYAIINEHWDNGWIQPTYADSA
                     YVKNRLAAMWKQIAIYFRDYDDHLLFAGTNEVMKEGNWGEPTKEYYTVQNIYNQVFVN
                     TVRATGGRNYYRHLLVQGYRTDINCTVKYFVIPQDVVPNRLMVEVHYYDPYNFTLNED
                     DKIIQWGKYATDASRTETWANESWADGQFNKMKTNFIDKGYAVILGEYCATSRLNLGS
                     AELNAQHAEYRRYYINYVTYSMVYHGLVPFYWDNGFTGNHGSGIFNRADGTQAYPNLV
                     KAIMDGVNLTVFPVGIKDEEKPKTAREFVLNQNYPNPFNSQTKISYYLPRTGKVTVKV
                     YDLNSCEVATLVRQELQKPGYYEVPFDASKLSSGLYFYQLSSEHFTDTRKMLLLK"
     assembly_gap    13879..13978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13979..16167)
                     /locus_tag="PLO58_01580"
     CDS             complement(<13979..16167)
                     /locus_tag="PLO58_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQO73511.1"
                     /translation="MKGIGLKIMLVLVASLLITQCSDSRKMVRQIDNFSQSWRFNLGD
                     LSDAERPEYDDSNWRTLDLPHDWSIEGEFSEEHPATPGGGALPGGIGWYRKTFTVSEG
                     NKDKSVFIDFDGVYQNSEVWINGHYLGKRPYGYSSFRYELTPYLNYGDENNIIAVKVD
                     NSQQPNSRWYSGSGIYRNVWLVVTDKVFVDHWGTFVTTPEVSSEAAKINIKTNVRNQT
                     GLDQEVILSTIIYDADGKLIARANTSQKIPKDTVIEIEQELSVKKPKLWSDERPYLYQ
                     AVTQISGAEKVWDDYSTSFGVRYFKFDPEKGFFLNGKPVKIKGVCNHHDLGCLGAAIN
                     TRAIERQLELLKSMGCNGIRTSHNPPAPELLDLCDRMGFIILDEAFDIWRVAKMKYDY
                     HLYFDEWHARDMADLVRRDRNHPSVFIWSIGNEVMEQWDKSNPSGMEIAQELANITRK
                     LDPTRPITAGCNDQNPENPLIKSNALDLIGYNYAHEKFADFPKTYPGKCFIATETHSV
                     IATRGSYDMPSDSIRVWPTRWDIPFTGGNPDFSCSAYDNCHVPWGSTHAETWSIVKKY
                     DFISGLYIWTGFDYLGEPTPYGWPARSSYFGIIDLAGFPKDSYYFYQSEWTKQPVLHI
                     FPHWNWQKGELIDVWAYTNYDEVELFLNDQSLGKKQKSDGVFQLVWRIEYTPGTLKAV
                     AHKKGSADLTKVIKTAGEPAGILLEPDRDVIKADGKDLSFITVKVVD"
     gene            complement(16185..19037)
                     /locus_tag="PLO58_01585"
     CDS             complement(16185..19037)
                     /locus_tag="PLO58_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQO73512.1"
                     /translation="MKISLRLALSLVLISGALLLADSRVQKLPNGVIIELAEEPAISP
                     RLIKIQVCDDDIIHVQATPESNFSERPSLIVEQAYANRLPESVNWTLQENEDTIILAT
                     AKLLVKISKTEGTVAFYKADGELILQERASGSKIFTAAEVMGEQTYHIQQFFDSPANE
                     AFYGLGQHQNGLMNYKGQSVDLWQYNIVVAVPFLISSRNYGILWDNNSRTKFGDIRDF
                     ESLATLKLYDKKGKPGGLSAEYFKDDGFKKLFTTRREPRIEHEFIDVNDPFPAGFDQV
                     ASVRWSGTIESAETGIYKFRLYCCGYTKMWLNDSLVVDNWRQNWLPWTHFPEIFMEAG
                     KRYSIKIEWIHQGGYIGLKYLTPSPEEYANSLSLYSEVADQIDYYFIYGNSLDEVIHG
                     YRKITGQAPLMPKWAMGLWQCRQRYQTQDEVLSVVREFRQRQIPLDNIVQDWFYWEED
                     KWGDHEFDPARFPDPDGMVKELHEKLNAHIMISVWPKFYVGTKNYQQFLEKGWLYMRN
                     VEKNQRDWVGPGYVSTFYDPYRPEARDLYWQQINEKLFSKGFDAWWLDATEPDIHSNL
                     SPEETRLRMHPTAIGSAARYLNTYSLVHCKGIYENQRRTNPDQRVFILTRSAFAGQQR
                     FAAATWSGDVAARWYDLKTQIPAGLNFCLSGIPYWTTDVGGFAVESRFVNATGENLEE
                     WRELMTRWFQFATFCPLFRVHGEYPYREIYHVAPEDHPAYQAMLAYDHLRYRLMPYIY
                     SLAGMVTHNDYTIMRALIMDFSQDTKVQNVGDQFMFGPALLINPVTEYKARTRSVYLP
                     AGTGWYDLKTGKYFNGGRTIIAEAPYSDIPIFVKEGSIIPYGPEIQYADEKSADPIIL
                     YVYTGRDAEFELYEDEGVNYNYEKGQFATIPLVYSEKTQTLTIGKRQGEFPGMLKERT
                     FQIIRVSPDKMSGLGFTLEPDKIVKYDGQEITIKIK"
     gene            19614..20828
                     /locus_tag="PLO58_01590"
     CDS             19614..20828
                     /locus_tag="PLO58_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQO73513.1"
                     /translation="MSLSFAENLQNALEEFQTGTQRFEEINHHLAKNLAWKKEAYEHL
                     KNLLLHMVKIGASDINLGGPRTNDKVWYRVYGNLEPSQEVPTYTNDEVAAIALSILSD
                     QQKTILFNERNIDFAFAFSTDQSQKPYRFRGDIYYERNNLAINFRHIAEKIFPIESLE
                     IPEPIIKRLDLKHEKSGLFLVTGVTGSGKSTTLDSITDMNNHNNKAHIVIIGKPIEYV
                     HKSDKSLITHREVGDDVLSFRNGIIEALRQDPNIIIVGEMRDPATISAVLEATDSGHK
                     IFSTLHTSSTVESIHRIIAEFPPDEQERIRFRLADTLKVVISQKLVPNKEGKRTLAKE
                     ILSVDYSVQAAIRNNNITEIFQMITEGKNKGMFTLQQDLYRLVRNGTITTETALNYCN
                     NKKIMQQLLGFS"
     gene            20905..21462
                     /locus_tag="PLO58_01595"
     CDS             20905..21462
                     /locus_tag="PLO58_01595"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQO73514.1"
                     /translation="MYIFGNLKAGFLNAWLGVLLIFLASWTILATDKKARRRLSNTSW
                     CSPREKKLVWSGTFLLFVILVFSIFVPLQLNTNWFYIGTAIFLVGYICLVISYFNYIK
                     TAGDKSKTKGFYNISRNPIYLFTSVALLGISIASASLIMLILVIIHFIISHFVILAEE
                     RHFLHIYKNTYKKYMKRVRRYFLFF"
     assembly_gap    21579..21678
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21783..>23464)
                     /locus_tag="PLO58_01600"
     CDS             complement(21783..>23464)
                     /locus_tag="PLO58_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO73515.1"
                     /translation="EGNYHLAISPGIYEVTSSLDGYTTVTLKDIVVEQNQVTEKIDMT
                     LIDWTPISGNHYNMVVVARAKISGKTIGGGNNNQIAAFGSGGDEDCRGIGSWIEPSPP
                     YDGYWYFTIRGNEIGEVIGFKIYLEELNAIETIIQTISFEDNATIGTPDEPLELRNFA
                     KQEFSLQKDWNWVSFNIHPDNPLIDSVFSSLTSSIFQIKSQNKSATFYRAQNKWMGDL
                     TQIDDGKTYLIKMDQAVEKFSVVGTPINVSTPIELIEGWNWIAYYPQNSMAVNSALQS
                     IMPNAFQVKSQTQSATYFDPPSTWVGDLSLMEPNQGYKLKVNSPSTLIYPENLLAKSL
                     SKITDIADSPDWHQITGTQYNMVLVASVKLDNEKFDSSNGNLVGAFGPGGEEDCRCVA
                     TWQKPVDDSDGFWYFTIVGDEHNEEISFKLYDGKNDRIINCNQTIKFVVDSTIGMPPE
                     FFIITANTSDILNPGIQTKFKLAQNYPNPFNSTTQIKFSLEKQSNVNITVYDLTGQKV
                     RTLLDAKLNLGEYRVVWDGRDDWGMPVSSGIYLYRMKADNYSELRKMILSK"
     assembly_gap    23465..23564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23848..>25775)
                     /locus_tag="PLO58_01605"
     CDS             complement(23848..>25775)
                     /locus_tag="PLO58_01605"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQO73516.1"
                     /translation="DLPAGTYRVTAALTGYETAVDTIAVTSGAPTTVNFSLKYVVVEG
                     TISGKVSLKVAGGSLTNVVITVSNDEKTYHFTDKVNAAGDYEVTVNQGVYDVTASLAG
                     YAPVTINNVQVVANNVTPNIDMRLINWSKITGTEFNMILFTTVSLEGDFFTKTKSDQL
                     AAFGSGGTNDCRGIATWKEGNHPYWDNSWDIAGYWYLTIVSDNNTNDTISFKIYDTET
                     DDVYDCNEKIIFADCQIMDTVLTIDGSSKEQKFDLVKGWNWVSFNLSPKEASIDSVFK
                     DLTDASLIYQVKTQNSSATASKSGNLVGDLTTVNNSDGYLIKMTDAFNSFTFQGQLIN
                     PVIHPISLDQNWNWIGYLPQNESPLNTALASIIGGDTIIVKNQSQSAICIGNTWIGDL
                     TKMAPGIGYKLKITTDDSVTYLTYPFVSGALSKPLTQKELASNLAGWKVMSGNKSNMI
                     ALVKPEIDAEKITDSERYTIGVFDSRGNCRSVGKKEENCWYFTIVGNEDGESLNFKIW
                     DAKKECALTADQKIQFVSDALVGAPSEPLVVKFSGSEATDVSLTLPTKLTLEQNYPNP
                     FNPVTAISYTIPEPGIVNLKIYDLKGNLVKTLVDKFQDSGNYTVEWNAGDLSSGIYFY
                     KIQYGGQSLIKRCLLIK"
     assembly_gap    25776..25875
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25876..26975)
                     /locus_tag="PLO58_01610"
     CDS             complement(<25876..26975)
                     /locus_tag="PLO58_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQO73517.1"
                     /translation="MAKKFFSASLILLVFSGLIFGAVINVPSNQPTIQAGIDAAVTGD
                     VVLVAEGTYKEKIDFKGKKITVASLFYNDGKTDHIQKTIIDGQATGTVVKFSVGEDSS
                     SVLIGFTVTNGSADYGGGIYCYNSSPTIKNVVIRDNVAKYSGAGIHCYSSSPTLERTV
                     IYNNKANESGGTGGGICCFNNSNPVLRNVTIAKNTAKSNGNDIACQYASKITIVNSII
                     WSEYLPDPIYIEGTGSISATYSDIKNGTGKSYFGEGCIATDPLFKDYNNNDFTLNYNS
                     PAIDAGNPDLDADGTSWTDDVDDQDPDGTRLDMGAYYFVRTGIQGTITINGGGGKVED
                     AVIKATLTTAPSTSSTTKPDKDGKYFLQLSNG"
     gene            complement(27188..29263)
                     /locus_tag="PLO58_01615"
     CDS             complement(27188..29263)
                     /locus_tag="PLO58_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73518.1"
                     /translation="MKNKLKITGLFLFLAISLVVIFDCGKITSPGGIGIPENPRIKTI
                     DDNNIEVSWDYDQSDQDVFFTVARKVGDANWNLEYAQTYDNFLIDEIPTNDSLIYAYK
                     IKTESDTSELASEFSDVVAYFSPKTIPTEIAISVLESDKVEVTWRDNCIGEDGYKIEK
                     KVSGGNWIKDYKTLGRNVTSFTDKTSFGDSVFYRVYAFCGISVSGSAEGFGISTFPPP
                     YNLEYERIAIDKIKLTWQSKTSNVAGFKIDKKVGSADWQVEIATLDPNQTWWIDENAE
                     INEILQYRVYGFSEEYISSYVTTDEIDNTFPAPSNLTLTKIDEDIVKISWQDNSQGES
                     GFYIDRKVGMSDWVNGYASVDSNQTTLLDNISEPCGNFYYRVRAFKNQYFSEPTDEGN
                     INIRLNLVSSLTTEGTATEVFVSDWKAYVANHYNGLIVIDLINPSSPSTDNNLVLPLP
                     DRTLSIYIYKNLAYATNHTGGLNIVDVTTLELIGNCQTSGVPVDVFVTGEPPYAYLCE
                     EEKGVSIASILSSTPHIISNITPPNTSADEKAKKLFVANDYAYVAYGSRGLTIFDVSD
                     VKNPIYVADFPSSSITQDVFIKDNYAFLADGENGLVIVDVSSVEAPSLVSNCPTHGFA
                     YSIYVRGNYAYLADKEKGLIVVNISDLANPFILGSFEMSTTPNSVFVDGSYAFVTDSE
                     GLKIIQIAP"
     gene            complement(29326..30513)
                     /locus_tag="PLO58_01620"
     CDS             complement(29326..30513)
                     /locus_tag="PLO58_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73519.1"
                     /translation="MKKKALFICLLLFFSSTNSQVNLIKSPSSDELITISPEVLVADA
                     LKMIEALSQKYEGRKIVNMSTYTGPISIPITQIYWKNALNLIANYNRLVVEELPGAYV
                     VKDFIAEEKAVQEKKPADEVVPDTKQVRISSIFFTADRSIINSLGIDWSTIYNGTVVA
                     SLDFTGGSEVLSDIISGTVSTTLESGEAIIELNALFKMIEHYNKGTIIARPSVTVISG
                     KQGYLQIGQDFSVRTTDFSGNLVDRFYSAGLILEVTPTIIQDSSKEAIHLVARVEKSS
                     VVPSDVQVIVNKNQATTEAILYDGEETVVAGLYDTEDIVVRKGVPILKDLPWWVLGLR
                     YIFGYSNHEKNEREIIIILKAELIEPIEVRMAKKESARELIEKYYKPKEHIDTLLFKK
                     VQE"
     gene            complement(30517..31383)
                     /locus_tag="PLO58_01625"
     CDS             complement(30517..31383)
                     /locus_tag="PLO58_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73520.1"
                     /translation="MPYKIRNTIALAVILLIVLIGSVISNSSSERKIADLSKKNQELS
                     KRLNMLNSQLENIGEEEELEQEIRELEIEISKFKKAIVKEDNPTITYDYLTQICQKFC
                     PEMVFDFKPAESGEIQGTKYNDYLIAGIVPFNSLYTFIYQIENQPKLYLLELLEIREQ
                     PASEDIIPFAFKLRAFYDENGIEPESIPIREIKTVNIRFNPFYPGIHDPVQIKEEEQY
                     LNPEEAILVGLTPTIAFFKDKNGRTISLTTGEKVAYGYLSKIDWENQAVTFKINRIGV
                     YVEKTIYLQGKG"
     gene            complement(31385..33544)
                     /locus_tag="PLO58_01630"
     CDS             complement(31385..33544)
                     /locus_tag="PLO58_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73521.1"
                     /translation="MKNYYGIFQDGLDIQIAHLVRDEDLIKIQNLGETTLSEPLYNKK
                     EVNKIPTIELSDTNLEIQNNPDLKEEGFDLPEISEFDEFESNKLTEEITEVNEEGGTS
                     TSELQRFLSNFSLETGKIALNANEEQISYHIFNSSLTLSAIKKRIRTEILSQEELKSR
                     KYAIGCIPNYDGTTLAFLHRGEFDLLNAILQVRPLLSPKRYIFSYIDTNEIALMNLIR
                     ANYNFPENDYVLILYVGSEYKAGIVMRNNTHVKTFKIIGDTSSRSENIRQVIYSKIIL
                     EQDVSNIPPVQHLILTGNMVRDEDIRFFSSKFGETTEVSRFEFKNINIATINRKYSPE
                     NIAKYAIPISLALKAADPKNKAFYNTNLLPPKIIESQKRFKIGWHGFILFIAIFYFTY
                     SATLKNLENNRQIVELQRQNYNIESELRHKNLMFARANELSQQITGKRKSMDKIKTIS
                     GNKSQWHRTIIALSDAFAKNSISWINYLRSDTSGFMVQGTTNHKENIAYITRYFPGCI
                     VSDVKTSEIQSIPVMRFVMNFSYSDQEEWVMKVAKPAPVTPQSGKTAAPPPITPAPKS
                     IESESAPKLAAPAPAVVQSQSPPDQSTPRITPYTVFREAAETYLSGNIQEGYHKLTQF
                     VEKYPNSPFTYSANYLIGECLFLMGEVAQAKDIFEKIANQDGAKTPDALLMLGRCYNA
                     LGDKAKAIDCWGMLITKYPEHKLAKMAEEKIKYVTEH"
     gene            complement(33574..34800)
                     /locus_tag="PLO58_01635"
     CDS             complement(33574..34800)
                     /locus_tag="PLO58_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQO73522.1"
                     /translation="MGLSFTPEMEIALPEYLIGTARFKNINKLITFNNAWKKEAYDNL
                     KSLLKKMEEIDASDIDIGGSMLDVKIWYRVYGRKYPDLEAKRYSNDEITAILLSILSD
                     DQKIILFDKKNIDFSLGISFGEKEEANRFRGDIYYDNDVLVGNFRRVNRELFPIENLN
                     IPEPVLKRLNLRHEKSGLILITGITGSGKSTTLDSIINLNNEENEGHIVIISDPLEFI
                     HKSKKCIIRHREVGGDVLSFREGTIQALRQDPDIIIVGEMRDAPTIATVLEATDSGHK
                     VFTTLHTSSAVESIHRIIAEFLPEEQERIRLRLADTLSVVISQKLVPDRDNKLTLVKE
                     ILSVTDSVKAAIRNKNIGEIYQMITEGKKYGMVTMEQDLHNLYTKKIISRETALDFSN
                     NRKRIEQLLAYREICV"
     gene            complement(34896..35753)
                     /locus_tag="PLO58_01640"
     CDS             complement(34896..35753)
                     /locus_tag="PLO58_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQO73523.1"
                     /translation="MQSHILVVDDDKTIRMLCKEILEEAGYKVTLAADGEEALEKMDY
                     DDFDLYLVDMVMPRMDGLELMKKIKQKQPLAVVMILTGFSSIEGAIKAVHAGAFQYLS
                     KPINIDELLNAVKEGIKYSQDLYGPLLKIFEPGTELVEKGEMAILSGFSEEDKQDFMI
                     LSSIYNFEPGENIRIADKMASSIILIESGEVSVWLSNTSVDYLKKWDTWNEESFVINS
                     PTSSVLRAETPVTARFFERKKLLEFFAFKGEKLLKKFMINLINCLYIKWRKSIQRIIM
                     LKLVTTESE"
     gene            complement(35760..36038)
                     /locus_tag="PLO58_01645"
     CDS             complement(35760..36038)
                     /locus_tag="PLO58_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73524.1"
                     /translation="MSDQEELIRKYEEVVEVAASIASIDHDINNQLMVFALVERRLQN
                     AAQQYNDEKLLRSSQQVSEALDNIKRILSRLQNLKKLDLIKEKREKRE"
     gene            complement(36431..>36674)
                     /locus_tag="PLO58_01650"
     CDS             complement(36431..>36674)
                     /locus_tag="PLO58_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73525.1"
                     /translation="TTKWQGRTDESYVTVGGDINQSTYTGFLANLLNEVINRLNCTDT
                     KLDEVKILNEALDDDDIQDLVSLGGIKDLRLAYWIQ"
     assembly_gap    36675..36774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<36775..37402)
                     /locus_tag="PLO58_01655"
     CDS             complement(<36775..37402)
                     /locus_tag="PLO58_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73526.1"
                     /translation="MNSKNLRDFALTYSLKKAYELDDDEDWHLAGFLRERLDVDFSDK
                     LSDFFGGTIDSIRYTALNPAGDSIRISSFITQHIGKGVKAFETEAMVRFEIEEHPKSE
                     IFGFDFDESNPDSVFDGSKNDNNGILGNGVTNYKPTRTVGKNGSALDFDGDNDYVYVG
                     ENLIEPDGDELTVGTWVNFSNTNTDWGILAAEKKSNSDHNPIWSVRARK"
     gene            complement(37562..38137)
                     /locus_tag="PLO58_01660"
     CDS             complement(37562..38137)
                     /locus_tag="PLO58_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73527.1"
                     /translation="MTSLLDAIFATSIGGLFLITIFTTLFNLQASSQDTTFHVTLNNA
                     AEVQYQELDNCLGKVGAGVYSGQILVAEPDSFVFKTKYDLINDIMGSVINTISISLGD
                     STSQGIPIKIMQNGVPLPGSNVPLWLRDLNFSYYDIDENQITTPGDSLSYIRLLKTDL
                     QFAFETSYVGPGERNIITNLTFWKYFINFYL"
     gene            complement(38134..38553)
                     /locus_tag="PLO58_01665"
     CDS             complement(38134..38553)
                     /locus_tag="PLO58_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73528.1"
                     /translation="MGYPQMFALIGSIALFTSVVLTMNTSINNQRQIVYQSIYMNQAV
                     KLASKVFDKITVETKTDLIDFDDIAITYSGTDSLRIWGNDVWYIYNVSSHYCDSSGVD
                     AAGKTDFQRVDVKIWTNPFVILDTLKFNNIYYNIELV"
     gene            complement(38556..>40295)
                     /locus_tag="PLO58_01670"
     CDS             complement(38556..>40295)
                     /locus_tag="PLO58_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQO73529.1"
                     /translation="GIISSEILEQALKIQNEGDASSSKRLEDILVSNFKIDHDLVFGT
                     LAELYAFRTYRVNPEKLDPAQIKHTKELLSKFPDELKNQLLYYKVLPYQVINGSRNKL
                     LVLASNPTEKVVEKIPLFTEFKKYEVVYTPLKIVEELIDLISPRENEFLDLLREAVVE
                     PEISEEKQVTELDEYSLDEEINKSLLVNLYEGCLIEAVRRRASDIHIVPYEKNSVDIY
                     FRIDGRLQLWYRQDNVSPEAMAAVVKDRTIGVDRFERDTAQDGFFQRNVDDYLIRYRV
                     SVLPIISEEYERRFESIVIRIIDDRNVIADLRQLGFLPQAEQDFIKAISKSKGIVILT
                     GPTGSGKSTTLLAALHHVMDASKNILTIEDPVEYAIKGARQLKIGRKLSFEDAIRTIL
                     RHDPDIVMVGEIRDKITADVAVKLANTGHLTFTTLHTNDAPSAISRLYKMGVEPFLLA
                     YAINIIVAQRLVRKLCPFCRVPISSEKYPAALELGLTEEDLKSGKIFDAGTGCKKCNG
                     GYKGRISLAEALYFSPEVRKSILNSGAEVDEAEIREIAEGQGMLSILDSGLEKIREGV
                     TTVSEVSYAASEG"
BASE COUNT        11454 a         8992 c         7374 g        11875 t
ORIGIN      
        1 atttgaccgc ccacactgaa ccactccgtc aagcgcttcg cataacccag tcctatattc
       61 caccaaaata aagcataccg ttgctggcgc gtaaaaccga ttcacgataa aaattgtcct
      121 gcccgaatcc atcattgcga tcaaaattgt gaatttcacc attggcagga ttcagacgag
      181 aaataccatg attggttccc atccagatga aaccgtctcc atcttcaata agactctcta
      241 tgatgttatc gggcagacca tggcgttgat cataccgccc aaagtgcgca atgcccgaat
      301 ccgcttgaaa atattggaca taattcaaac cacctcccat tgtaccaatc cacagagtcc
      361 catcctggct gacaaacaag gtattgatat tatcatgtcc cagactcagc gaatctttat
      421 catcaggacg gaaaaatagc attttttcat tgttcggatt gaagaatacc agccctttac
      481 cataggttcc cagccagaat ccgccggatg tatcttccgc aatagaagtg acaacgttca
      541 gacggtcatt aatccgatct atgggatatt ggctaaaagt ctgcccgtca gaattcaatt
      601 tagatagacc gccgccccag gtgcctaccc ataaggtatt agatttatcc aaatagagag
      661 cgtaaacggc attttctcca agagaggaag gattttgatc atcatgccgg aaatgcttaa
      721 actggccgct tttgaaatcg taccgatcca gaccactacc gtcagtacca atccagagat
      781 aatctctatt gccagtcaaa cataaaatcg catccgaact gagaccatta ttctgttcat
      841 cttttcggta aactcgaaag tgattcgtaa ccggatcgaa gcgattcaga cctccgaaac
      901 gagtgccgat ccaaattttc ccatattgat cttcaaaaaa tgaggtaaca tgattattcg
      961 agagactatt agccccagaa agtccaaaac ggtaatgcaa aatatgactc tgtttcgtat
     1021 cgcatttatt gattccgcca ttttcatttc cgatccaaac cacaccagtt cgatccagat
     1081 aaaccgtcca gagcgcattg gattggatgc tcgtttcgtc aagaggttca caaagataat
     1141 gttggattcc ttcaatgccg gcggtggtaa catttctcgg gatttgaggt atgacgaata
     1201 atccggcgtc ataagtagac atccacatcc ggtctgattg atcaattaat aattttgtga
     1261 ttattttagt agtaaacggt tttttacctt tgcaaaaaat aggtactttg cggaatcttt
     1321 gtgcattgac atcaaatcgt tgcagtccgt caccccaggt gccaatccag aggtttcccc
     1381 atcgatcctc gccgatagta ataacggcgc tggtgccggc cttgggtgat tcagtagttt
     1441 cgtgacgata acgagtaaac gattgagtta tgcgattaaa gcgataaaca ccttcttttt
     1501 cggtcgcaat ccagagctga tcgcggctat cattataaat gtcatgaata ataaaatcac
     1561 ccaagccatt tggattattc ggatcgggaa gatagttctg gaaagtatca acccgaggat
     1621 cataacaact tagcccgccc tctgtgccga tccagatttg cccataatta tcctcggcaa
     1681 tacaacgtat gcgattatca actaacgaat tgggattatt aggattatga cgaattctaa
     1741 taaaacaatc ccgtgcccga tcgtaacgat tcaagccgtc gagagtgcca atccacaaaa
     1801 ttccacgcct atcttcatat aaggcgttga ttacattatg gcaaaggctg gtcgtatctc
     1861 ctaaaatcgg tcggtaagcc gtgaaattat atccgtcgta tctatctaaa ccatttaatg
     1921 ttccaaacca cataaatccc tgccgatcct gaatgatctc gataatggaa ttgtgggata
     1981 aaccgtcttg agtagaaaga tgctcaaaac gcaaagtcgg taatccggca ctgaaggcat
     2041 tgctacttaa agccagcaga attggcgcaa ttgtcaatcc tgatattttt ctcagaaaac
     2101 tacccataag attcaatgac catggtgatc atttattaat tcaatttaat ctcgggaaat
     2161 taactccatt ttagttcccc aactgataaa tagaataact gcctcccgga attgtcagct
     2221 ggtaatattt gtcttccaac ttgagttctg actccgttcc agctaataat tcaaactgag
     2281 aaaacaccgg tttgagacgg aatcgggcgg tcgcggcttt attagccgga tttaatataa
     2341 ccagcacagt ttcgtcctca caagcccggg cgtaaataaa agggtattct ccttttttgg
     2401 cataaaccgg tacaaattcg gcataggcgc gtaaggcttt cgaattcttg cgtaattgga
     2461 tgaggttttt tactcgattt aacaatgaat taggatcatc ctgttgatcg gctacattgg
     2521 gtgcatcagc ggcaggatca accggcaggt ataatttttc tggattagcg gtagaaaagc
     2581 ccaaattttt accttgagac cattgcattg gagtccttgc cccagctcgc ggtggatagg
     2641 cgccctcgac attcggtaaa tcataaagct ggcgcattcc aatttcattg ccataataga
     2701 taaatggaat cccaggcatc gtcagtgaaa aagcaaaaat gatctccaga tcatcgtcag
     2761 aacgttcgat atttaaccgc gataggtcgt gatttcccag tggcagatta atgtagccct
     2821 tgccaactgt ctggcgatat tgctctaaat aacttgccaa aaattgagta atatcaccct
     2881 tgccttctcg atcaaaaaaa ctatgccctt cggaatggcc attcataatt cgccaactct
     2941 ctttctgcag tagatcgttg taacctgaaa accagtgaaa aaaatcggcg tggaaaccac
     3001 ctccatccag agccgccacc ggatatgacc attctgctac cataaaggct tgaggatatt
     3061 cttcgtctat gatctttctg attttctgcc agaaaatttt tgtgccttcg tcacgattgg
     3121 catattcagg atcactttcg acgatattgg cattcttgac caatgccccc gccatatcgg
     3181 ctcgaaaacc gtccgcaccc atatccatcc agaaacgcaa aaccttctgc aattcctcct
     3241 gaagtgccag cacatccgga tggtcaatcg gtaacatcca atcgctctcc ggtttgacga
     3301 atccgaaatt caaagctggc tgactccagt aaaaattgcg cataaattgg ccattccgtc
     3361 ggctatagcc tttaataaaa gcatcgcgat aggcttccgg cggatcaatc caggtattat
     3421 tggtccagac gtaccaattc gaatatttat tgggttcctg acgagcagat tcttgaaacc
     3481 aggggtgctc gatgctggtg tagctgatga cataatcgaa aaggaccttt agtccgcgtt
     3541 tttcggcttc caaaaataaa cgccgggcat cctgattggt accgtaacgc ggcgccactt
     3601 ggtaataatc ggaaatatca tacccggcgt cattaaaagg tgatgcgaaa aaaggattaa
     3661 tccagaatcc gtcaacgccg agactctgca catagtccag tttttcaata atcccattca
     3721 aatcaccaat gccgtcgccg ttggaatcgt aaaatgtctg cggataaatt tgataaaaca
     3781 cggcattatg caaccattca ggaccaggcg gcacaattgt ttgcccggtt atttctgccg
     3841 aatcaataga aatatccact gcggacttat cttcactaca tcctacgatt agcattaagg
     3901 tcaaaataag tgtcaccatc cgacgatgca taaattcctc cgatcatttc aggagttgat
     3961 caaactcccc gtactttcac ctgctctttt accatcaatt aaaaagaaat ctgccaataa
     4021 tattcccttt gaaaaacagt tcaatattag gatttatctg gttatcaatc aaattttatt
     4081 ataaaaccac cgtgtttttc cagcttaata gataaagggc tttgaggaga aacatgaatt
     4141 tctttcgtca taaaagaacg cccatcggac gcatcagtaa ttaattgacc tgtgtggtct
     4201 tcatctaaaa agttacaatt taatttcagc tgtctttcga catcggcgcc atttattcca
     4261 gctaaatacc aggtttgccc tgactttctt gccagcacta caaatttgcc cggaaaaccg
     4321 tcaataaaat aagtctcatc ccagctaacc gggacttctc tcataaaatt tttgatataa
     4381 tccggaacac acgccatacc ggccggaatt tccgcaaaat gctgaattcc cgaccagaat
     4441 ataaccgcag tagccaattc aaagccattc gtggttatcc gtcgaatatt tggtatttcc
     4501 gaaaaacaaa ctggggtaaa atccatcgga tcgaaaacat tacgcgtaaa aggcaaaagg
     4561 caagcgtggg tcggctctag atcggcatca gcctgatcga aagttataaa ttcaaaaccg
     4621 cgcaccgcct ccatcgttag caggttggga tacgttctct gccaaccgcg tggtaaggta
     4681 gcgccatgac aattcaccac cagaccgaat tgcgccacat cttcaaaaat gtcctgataa
     4741 taggtcatca ttgattgccc atcaccaccg aagaaatcca ctttaatccc gcggattccc
     4801 atctttttga ggcgtgcgaa ttccctaagg cgatcgctat gagtcagcaa tttacttttc
     4861 gggctgtaag ttgtgctatt ccaatcgccc gaagaattat accagagcag aattccgacg
     4921 cctttggttt gggcgtaagc cgccagctcc gcgactttat cataaccgat tttccagtcc
     4981 caatcggcat ctatcaaaca atattcccaa ttcatttcag cggcataatc aatgaatctt
     5041 ttttgcactt caaaaactgt ggaatcatct ttaagaagca cccagctcca cgacgagcga
     5101 cccggtttga catagtcaat ctccttcaat cgagccggtt cggccagatc cgttccgaga
     5161 gttgattcga caatattttt caaccctttg ccgatcgtaa taatccgcca gggggtgctc
     5221 catggtagag gtgaagtcgg taaccaaatc ccacccggaa aagtttccaa atccgatgga
     5281 aaatcaatat aaaaaatatg actcgtcgaa tcctgttgca accggcatcc gcagtaattg
     5341 cgatcgggcg cagtttcact aatcagcatc caggaatcgc catcctcgaa gagcgccgga
     5401 aaaacccagc cgggttcgtt aaatggcaat ttatctatcg caacattctg ctgataaatt
     5461 tcctcatagg aaggattggt ctggcaccag cccgatttag ccattgccat cggttgaatg
     5521 aaagctttag ttcgaggtga aaaatgaaat tcgctgatct cccttaagat ggttttgggt
     5581 tcatccgatt gcccgggaaa aatgtaacga aaggctaccc cgtcattcga ggcgcgaaaa
     5641 accacatcta ttttttgatt cacgtggttt gtcagataaa atctcttttc attgccgttg
     5701 taagcataat ggcttttttt gccttgtggc atttgataat tgaaattaat cgggcgggag
     5761 gctgagaccg aatccagctt tagaccttcg ctaaaatcag catcttctcg aacaataccc
     5821 attcgggaag cttgaacaat ttccttgttc cgataagcaa cgcgatagaa aattttccca
     5881 gccggcgata tttcaaaccg aaccgtaata ttgccatctg gacttttcag atattgattt
     5941 ttcggcttgt tagagctaca agtttgaatc atcgttaatg aaattaataa tgtcacaaac
     6001 gaacgaatga ttttcgcgct aatcattttt ctcctcatat taaatatttt caattataaa
     6061 gtttgccgct attttaccgc aattttgaaa ttcagattcc tatcaactat aagattcatt
     6121 gacaaacaaa tttaacggtg acatcactgt caaccggcag aaagatctga aagtttctat
     6181 catcattcgg caatcgagtt attaagtctc gatctgggcc aagaatccgc ccaaccggtc
     6241 gcggcatcct caattctaaa ttttgattca cccgcgattg taaagtcact aatatctgat
     6301 tctcccgcca tttcagttct tttatttcga tttgtccacg caccagaacg ccactaatac
     6361 ttccggcagg ccaggaatta ggcaaagccg gcagtaattt gatcattccg ggctcagaat
     6421 agaccagcgt gcgaatgacg gcggctggga aaccgccgct taaatccata ttgaataaat
     6481 tgcccggatc atgcagactc gccaatgaat tgctccagta atagctcgcc atccagttga
     6541 tcagttcctc cgccatctca gcgtcaccca ggttggcggc taccaatgcc atttgcacca
     6601 agccaaagac cattgcgcca ccattttcct gccgtctgat tttcatccgc tcggctatcg
     6661 cattttttgc agattttata agttcaggat taccggcaat atcaggatca agcatttcat
     6721 ataatccata aagatgcgaa acgtgccgat ggttgtaatt gtcttccagc ccgggccata
     6781 accattcgcg gagagcgccg tcttcattga tctcatactc cggcattagg gctagcattt
     6841 cttgccattt aattgcttgg gattcatcaa ccttaagaat tttggcggct tgaatgcaat
     6901 tgcgcagaag ctgtttggca accataacat ccattgtggc attgatgcaa gcttgcgaat
     6961 tggaattagc cggattattc tccggggagt aggacggatt gaaaacatat ttgccattct
     7021 ctcctatagt cagaaaatct tcataaaatt gcgccgccgc tttcataaac gggtaagccc
     7081 gctctttcaa aaaagtcgtg tctccggtat ataaaaagta atcgtagaaa aaactggaga
     7141 cccaacccgc tcccgccgtc cagaaagtca aacaccaagt ttcgccaaag tgaatatccc
     7201 aaccgtgtga actagtgcgg gatggaatat gaattccgcg acaaccatat aaacggcggg
     7261 cgttatcttc ataaaacggc aataattttt cgtgataatc gaaataagca agcatcaatt
     7321 cgggcaaatt gccacatagc aaactggaaa tagccacctc ggcattgcca tcattcgtaa
     7381 aatccgatga ccagggaggc gaccaagtgc cattccaaat tccctgtaaa ttcggcgggc
     7441 gcgttccagt ggcgcaaaga atattatagc gcgcggcgtc gaagaatttc tcgatcatag
     7501 gcggagtaac ttcttcacgg gctctggcaa ccagaacttc ggattgcagt gtttttagtt
     7561 tgcgaccgcc caaatcgagt tttacccgat tatataaatt gccgtgaata ttcacatgac
     7621 gttcgaggag tgttttgtaa gtcgtgggct gattttgtaa agctgattgc aactcactaa
     7681 ctcgggaatt ttcccaatca aaattcggtt ccaaccgcac caaaagcaga atttcatcgg
     7741 cattttgaat gaccagctgg tttccttcac tggataaatt tccgcctttc agaatcactc
     7801 ttccgacggc ttcataaccc tgcggattgt catcataatg gcgtttgaac ttacagcggt
     7861 aagttagcca acagtccttt gctccaattt cggtaaactc aatggcgcca ttgaccattt
     7921 cctgttgact ccattcgact gggtgcatag caaattccag gctggtgttg attttaccgc
     7981 tacctttgat tgacatcact atgcaattgt cggctcgcga aacgaacaat ttgcgttcga
     8041 accgaccgcc ttcatcaagc cagaaaacca tcgcttcacc agttgtaaaa tccactgctc
     8101 gagcatagtc gctgacgttg gcggctttca tattcaagcg aatattcaac gccggcatat
     8161 aggggtcagt ccagtgagtt ccctgataac cttctgtctc cccgatttcg acgggaatct
     8221 tggcggcttc tgcatatttt ccctgcaata aaagttgacg gatttcgtcc aatcggctgg
     8281 cttggttaat cggcggtaag cgatcactcg ccgggagata gagtaaagca tgatttaaaa
     8341 taatcgtttc atcatgcggc cgcccgatga ccattgcccc gatcgtccca ttgccggtga
     8401 gaagcgcctc ttcccatttg cttcccggta gccagctgac agagccgcgt tccggtaacc
     8461 tgatgcgctg tccacaggtc ggtaaacagg ctaaaaataa tcccagcgaa attgtcatta
     8521 gcaaaatttt tggcacgatt ttgatcttct cgaacaaagt atttctattt ttgattaatg
     8581 gtaaaaaatc taaggtgttt acagatagat agcattattt caccggaatt ttgcattcgc
     8641 tttccactaa tagaaattgc acggagtagg gcggtaaatc gaaaactagt ggcctggtga
     8701 ttttggtcga ataagcttta attttctcaa aatcggctgg accaccggct ggcaattctg
     8761 ggctaacacc atttatgaaa acttgcagtg aaaaatcacc gttgtccttg cctccggtca
     8821 gcagatacca gtaacagcgc cgtcccggtt gaaaattagc aaattccagt cgcaccgatt
     8881 tgggagaatt tcccttgttg accagaatta tcccagcttc gccggaactg aagctcgaag
     8941 cgtaggcaac cacatcgtta tcaccgacga catttgtatt gaccagatga tcgccacaaa
     9001 attgcttaaa gtaagtcata taataaaaaa ccggtcgggg attccagcgt ggcacattcg
     9061 gttcaccacc ctgcgaaaac atcccgtgat cattgccatc attccagtta ttcgctagat
     9121 tccagcgaca agccaggcca tatttatcct taatcaattc acccagcacc aggacggcat
     9181 ggatgccatt aatatagctg acctgctgtt tcgacccgac ggcaaaaata ttccactnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg ccattaatat agctgacctg
     9361 ctgtttcgac ccgacggcaa aaatattcca ctcggtcaag cccaccggtt tcatttttac
     9421 tttatttatt cgagcggtct cttgcataaa agtcatcatc tcttgcgtta ccggtatggc
     9481 ggaattcaga atcgttccca ctgaagaatt ttcgttccag ggtgtgtaat aggaatgaat
     9541 gacaaagaaa tcggccgcgt ctccggcttg cttaaaaaag ccggaattcc aggtcttttc
     9601 cgtcggtgta tgccaatcct gcgccgattc gagcaagctg gctcccagct tgatttccgc
     9661 tccgattgag tgagcggcgg cacgcattga atcagcaaaa accttgaaat gctgtccgta
     9721 taattcgccg gtaataattt ccggctgacc gtctttatta tcggctgtat ttattttata
     9781 tccagcctgc caattgccga aattctcgtt gccgatttcc cagtacttcg ttcgaccatt
     9841 atcatagcgc acccagtcag ccgcatattt agccgcagta gccaccggat tttcaccctt
     9901 gccatatcga gcataactgt aattcacaca aataattccg gtgcaattcg tcatttccag
     9961 catcttatag taattatcaa ccgaaagcgt aattgcggta tcgttccgcc cgaaccagaa
    10021 ctcttcgcgg tagggctttt cgacaccgcc ataaagcgga tccggcacat cctccggcag
    10081 ttggccaggc tgattattcc agaaaaatag attactcaaa tttccacccg gatagcgcag
    10141 gatttgcggc gcaagttgct ttatgtaacc aatcaaggtt ggttcattga ccatctgggt
    10201 catatagaca ttggcattat gaccgtagat atatttcggg accttggtca acgtatcgcc
    10261 aatatcaatt tcaaccgtca cggtcgaacg ggactgcgtt aacgtgactt ccctgaaagg
    10321 cggtatagtg gctttcttcg gctgaaaatc atatagaaaa aagccctgcg gaggttctgc
    10381 tccgactaaa atccggattc caaatcctaa aacaattata ctacgccaga ttcttcgcaa
    10441 ctcgatttcc ccctataaaa ttatcggttt tgtgtaactc tttaaccaat caattcaagt
    10501 tctatagaat ttctcgctac tactcatgat aatcttttaa gttatttctt ctatagcatt
    10561 tctttttcat atctaactaa ttgcatcctt tgccaaatcc gaaatcataa ccagtgccga
    10621 caactgcttc acaatagggg cagagaactt cctcctgagg atcagtacca agactatggt
    10681 gtgcggtaca ttcactatat ccttcaccat tccacgacca ctcttcggtc attatcgccg
    10741 taaaccaaat acgatctaaa tattcaccac aattgggaca aattctaata gattttttat
    10801 tattcattta gttaatacct ctatatttaa ttacttgctt tggaagctta atatattttt
    10861 ctttgacaat tctgcaagct gtaaaaaagg gcaaatgtct ttttatcttt cctgccagtt
    10921 tcttatcttc gaaaaattct atcaggtgat ttggcatttt ttataactcc ttctgtttat
    10981 gggctaattt tggatactgt ttccagtata gaaaaagccc taccgaaggt agaattttgg
    11041 cgagtgccgc aagacacaaa ccttttatat tgactaatac cgcgggtact cttcactaat
    11101 aatactcttt cattaggctc tttttatctc ggcagtcaca tatttgggtt ggcactttgg
    11161 caaatctaaa tggcattctc caaataaatt acagtatcgc ttgcaaattg aaacgagtat
    11221 tagcattacc ggaacttcaa tcaaaacacc gactacggtt gctaacgccg caccagaaga
    11281 caatccaaaa agcatagtgg cggtcgcaat tgccacttca aaatgattcg aagcaccaat
    11341 catcgcggcc ggagcggcat catgatagga aagttttaac catcgtgcta aaacgaagta
    11401 tcccaaacta aaaataaata tggtttggat aaaaagggga actgcaatcc agaatatcgt
    11461 cagaggattt tccattataa tttctccctt gaaagagaat aaaaggacca atgtaataag
    11521 aagtgccaac atacttattg gtgtgagacg atgcacaaac ttcgtattaa accattctaa
    11581 acctctggac ttgacaatca acttccgcga aaagtaccct gcgaccaatg gcaaggctac
    11641 ataaatcata accgaaaaca atattgtctg ccagggtatg ggcattgcat tgacaccaag
    11701 taaaaatctc ccaagagggg cataaagtac gagcattgat aaagagttta tagcaaccat
    11761 aaccaaagta agtccatcat tgccttttgc cagataactc cacatcaata ccatggcggt
    11821 acagggggca attcccaaaa gaattgcacc ggagatataa cttctccata actcaacttc
    11881 ttgcccggtc ttaataatct cggttccagg taagaatccc ttaaacagat gtcctagaaa
    11941 aaatgaagca atcagataca tagtaaatgg cttgattgcc caattgatga acaatgttaa
    12001 cgccacaggc ttaggcgttt ttgccgctct aactacctct gcaaaatcta tctttaccat
    12061 aattggatac atcataaaaa ataggcagat agcaatggga atggagacgt tatatattga
    12121 aagagaatcc aatttgaccg caatcccggg tgctattttc cctaatagga tacctgcgcc
    12181 gatgcaaagt aaaacccaga gagttaagta cttttcaaaa acagaaagtt ttctttcatt
    12241 agccattttt atttaccttc tttttggtgg cattttattt tccattcgaa tcaattatac
    12301 aaacaaaatg attccaatat caaaaatttt gcagacaact cgaattattt agagcggact
    12361 catttgagta aaagcatctt tctggtatca gtgaaatgtt cgctggacag ctgataaaaa
    12421 tataagccgc tggataattt cgaggcgtca aatggcactt cataataacc aggcttttgt
    12481 aactcttgtc gtacgagagt cgctacctca caactgttca aatcatagac tttgacggta
    12541 acttttccgg tgcgcggcaa atagtaggaa attttagtct gcgaattgaa ggggttggga
    12601 taattttggt taagcacaaa ttcacgagcg gttttcggct tttcttcatc tttaatgcct
    12661 accggaaaca ccgtcaaatt gacgccgtcc ataatggctt tcaccagatt cggataagcc
    12721 tgtgtgccgt cggcccgatt aaaaataccc gagccatgat tgccggtaaa cccattatcc
    12781 cagtaaaagg gcaccagacc gtgatagacc atcgagtaag taacgtagtt aatataatag
    12841 cgccgatatt cggcatgctg ggcattgagt tcggcactgc cgagattcaa ccttgacgtc
    12901 gcgcaatatt cacctaaaat taccgcataa cctttgtcaa taaaattggt tttcatttta
    12961 ttgaactgtc cgtccgccca ggattcgtta gcccaagtct cagttcgcga ggcatcggta
    13021 gcatactttc cccattgaat aattttatca tcttcattta aggtgaaatt ataggggtca
    13081 taataatgca cttccaccat caaacgattg ggaaccacgt cttgaggaat aacaaaatat
    13141 ttgacagtac aattaatatc agtcctataa ccctgaacca atagatggcg ataataattg
    13201 cgcccgccgg tggcccgaac ggtattgacg aatacctgat tatatatatt ctggacggta
    13261 taatattctt tggtaggctc gccccaattg ccctctttca ttacttcgtt ggtgccggca
    13321 aacagaaggt gatcatcata gtcgcggaaa tagatggcaa tctgtttcca catcgccgcc
    13381 aaccgatttt ttacgtatgc cgaatcggca taagtaggtt gaatccagcc attgtcccag
    13441 tgttcgttga taatggcata catcccgcta ctcaaaacgt aacaaaccac ttcttcgact
    13501 cgctccagcc aggattcttc gatggtaaaa gtagattcgt cggaaaattt gctccaggca
    13561 acaggaatcc ggatggcatt gaagccggcg gctttcacgg cgtcaatcaa cctctgagtt
    13621 atctgcggat tgccccaagc agtttcgcca ccgatggctt ccagtgaatt tccgacattc
    13681 cagccgggaa tcatttcttg agccaattca atggaagtaa tattgcgcat atcggttgag
    13741 tcgggtggga tataatcctg ccccaaaacc gacctaaaaa ataccagcag gataatccca
    13801 attgaaagag ttgggtattt tttgcgatta aatattaaag tccccatttt gaaacccttt
    13861 tcattagaaa taacttttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
    13981 atcaacgact tttactgtaa taaatgacag gtctttacca tcagctttta taacatcgcg
    14041 atcgggctcg agtaaaatgc cagccggttc accggcagtc ttaattactt tggttaaatc
    14101 agcagagcct tttttgtgtg ccacagcttt cagggtgccg ggtgtgtact ctattcgcca
    14161 aaccaattga aaaacaccgt cgcttttttg ctttttaccc aacgattgat cattgaggaa
    14221 aagttcgacc tcatcataat tcgtataagc ccaaacgtca attaattcgc ctttctgcca
    14281 gttccagtgc ggaaaaatgt gtaataccgg ttgcttagtc cattcactct ggtaaaaata
    14341 ataactgtct ttagggaatc ccgccaaatc gataattcca aaataggaac tacgcgccgg
    14401 ccatccgtac ggagtcggct cgccgagata atcaaaaccg gtccagatat acaatcccga
    14461 aatgaaatca tactttttca cgattgacca ggtttcggca tgagtcgaac cccagggcac
    14521 gtgacaatta tcataagccg agcatgaaaa gtccggattg ccgccagtaa aaggaatatc
    14581 ccagcgcgtc ggccagacgc gaatgctatc cgacggcata tcataactgc cacgcgtcgc
    14641 tataacggag tgagtttcgg tagcgataaa gcacttgccc ggataggttt tcggaaagtc
    14701 ggcaaatttt tcatgcgcat aattatagcc aatcaaatcc agtgcattag atttaatgag
    14761 tggattttcg ggattctgat cattacagcc agctgtgatg ggacgcgtgg gatcgagctt
    14821 tctggtaata ttagccaatt cctgggcaat ttccatgccg ctgggattac ttttatccca
    14881 ctgttccata acctcgttcc cgatactcca gataaaaacg ctggggtgat tgcgatcgcg
    14941 tcgaactaaa tcggccatat cgcgcgcgtg ccattcgtcg aaataaagat gataatcgta
    15001 tttcattttc gctactcgcc aaatatcgaa agcttcatct aatatgataa aacccatccg
    15061 atcacacaaa tccagcaatt ccggcgccgg cggattgtgg gaagtgcgaa tcccattaca
    15121 acccatagac tttaataatt ccagttgccg ctcgattgca cgcgtgttaa ttgcggcacc
    15181 cagacagccg agatcatgat gattgcatac gcctttaatt ttcactggct taccattcag
    15241 aaaaaatccc ttttccggat cgaatttgaa ataacgaact ccaaaagaag tcgaataatc
    15301 atcccagact ttttccgctc cgctaatttg agtcacagcc tggtataaat aaggacgctc
    15361 atccgaccac aacttgggtt ttttcactga caattcctgt tcgatttcaa tcacggtatc
    15421 ctttggtatc ttttgggaag tattggctct ggcaatcaac ttcccatcag catcatagat
    15481 aatggtactc aggataactt cctggtcgag accggtctga tttctaacgt tggtttttat
    15541 attgattttg gcggcttcgc tactaacctc cggcgtggta acaaatgtac cccaatgatc
    15601 tacgaatact ttatcggtga ctactaacca gacgtttcgg taaatcccgg atcccgaata
    15661 ccaacgcgaa ttgggttgct gggaattatc aacttttaca gcgataatat tattttcgtc
    15721 gccatagttg agataaggtg tcaattcgta tcgaaaagaa ctatagccgt acggtctttt
    15781 ccccaaatag tggccattta tccagacctc gctattttgg taaacgccgt caaaatcaat
    15841 gaaaacactc ttatctttat ttccctcgga aacagtaaaa gtcttgcgat accatccaat
    15901 tccacccggt aaagcaccac cacccggggt tgcgggatgc tcctcactaa attcaccttc
    15961 gatactccaa tcatggggca aatctaaagt acgccaattg gaatcgtcat attcgggtct
    16021 ttcggcatca cttaaatctc ctagattaaa tcgccaactt tggctaaaat tatcaatctg
    16081 tctgaccatc ttgcggctat cagagcattg agtaatgagc agtgatgcaa caagcaccaa
    16141 cattattttt aatcctatcc ctttcataaa tacccccaaa aatattattt gatttttatc
    16201 gttatttcct ggccatcata ttttactatt ttatccggtt cgagagtaaa gccaagtccg
    16261 ctcattttat ccgggctaac tcggataatt tgaaatgttc tttccttcaa cattcccgga
    16321 aattcaccct gacgctttcc aatcgtcaat gtttgagttt tctcagaata aaccaatgga
    16381 attgtggcaa attgcccttt ttcataatta taattgactc cttcgtcctc gtaaagttca
    16441 aactcggcat cccgaccggt atagacatac aaaataatcg gatcagcgga tttttcatcg
    16501 gcgtactgaa tttccggtcc gtaaggaata attgaacctt ctttcacgaa aatcggtata
    16561 tcgctataag gggcctcggc tataatcgtc cgaccgccgt tgaaatattt cccggttttc
    16621 aaatcatacc aaccagtacc agccggtaaa tagaccgagc gtgtgcgggc tttatattcc
    16681 gtcaccggat taatgagcaa agccggaccg aacataaatt gatcgccgac attttgaact
    16741 tttgtatcct gactgaaatc cattatgagc gctcgcataa tcgtgtaatc gttatgcgtt
    16801 accataccag ccagcgaata aatatagggc attaagcgat agcgcaagtg atcgtaggct
    16861 aacattgcct gataggcggg gtgatcttct ggcgcaacgt gataaatttc acggtaggga
    16921 tattctccat gaacccggaa aagcggacaa aaggtggcaa actgaaacca gcgggtcatt
    16981 agctcgcgcc attcttccag attttcgccg gtggcgttga caaagcgaga ttcgaccgca
    17041 aagccaccga catccgttgt ccaataggga attccggata gacaaaaatt caaaccagct
    17101 ggaatttgag ttttcaaatc ataccagcgg gcggcaacat ccccgctcca ggtcgcggcg
    17161 gcaaaccgct gttgtccggc gaaagccgaa cgagttagaa taaaaacccg ttgatcggga
    17221 ttagttcggc gctgattttc ataaattccc ttgcaatgaa ctaacgaata agtattcaaa
    17281 tagcgagcgg cgctaccgat ggcagttgga tgcatccgca agcgcgtttc ttccggcgac
    17341 aaattggaat gaatatccgg ttcggtagca tccagccacc aggcgtcaaa tcctttactg
    17401 aataattttt cattgatctg ttgccagtat aaatctctgg cttccggacg gtaaggatcg
    17461 taaaatgtgg aaacgtagcc gggtccgacc caatctctct ggttcttttc gacattacgc
    17521 atatatagcc agcccttttc cagaaattgc tggtaatttt tcgtcccgac gtaaaacttt
    17581 ggccagaccg aaatcataat gtgagcattt agtttttcat gcagttcttt gaccataccg
    17641 tctggatcgg gaaagcgagc tgggtcaaat tcgtggtcgc cccacttatc ctcttcccaa
    17701 tagaaccaat cctgaacaat attgtccagc ggaatctggc gctggcgaaa ttcccgcaca
    17761 accgaaagca cctcgtcctg agtctgataa cgctggcgac attgccacaa acccatcgcc
    17821 cattttggca tcagcggcgc ttgcccagta attttgcggt agccgtgaat cacttcatct
    17881 aaactgttac cataaataaa gtaatagtca atctggtcag caacctccga ataaagcgaa
    17941 aggctattag catactcctc tgggctggga gtcagatact tgaggccaat atatccacct
    18001 tgatgaatcc attcaatttt aattgaatag cgcttcccgg cttccataaa aatttccgga
    18061 aaatgcgtcc agggcaacca attctgacgc caattatcca ccactaaaga gtcattcagc
    18121 cacattttag tatagccgca acaatagagc cgaaatttat aaataccagt ctcggcggat
    18181 tcgattgtcc cgctccagcg cacactcgct acttggtcga atccagctgg gaatggatcg
    18241 ttgacgtcaa taaactcgtg ttcgatgcgt ggttcgcggc gagttgtaaa tagtttttta
    18301 aatccgtcat ctttaaaata ttcggcagat agaccacccg gtttgccttt cttgtcatat
    18361 aattttaatg tggctaatga ttcaaagtcg cgaatgtcac cgaatttggt gcgggaatta
    18421 ttgtcccaca aaataccata attgcggctg gaaatcagaa atggcacggc taccacgata
    18481 ttatattgcc agagatcaac cgactgtcct ttataattca tcaatccgtt ctggtgttgt
    18541 cccaatccgt aaaaggcttc attggcaggc gaatcgaaaa actgttgaat atgataagtc
    18601 tgttcgccca tcacttccgc cgcagtaaaa attttactgc cgctagctct ctcctgtaaa
    18661 attagttcgc cgtctgcttt atagaaagca accgtacctt cagttttcga tattttcacc
    18721 agcagtttgg cagttgccag aatgattgta tcctcgtttt cttgtaatgt ccaattaacc
    18781 gattcaggca accgattggc ataagcctgc tccactatca gactgggacg ctcagaaaaa
    18841 ttggactcgg gtgtagcctg gacgtgaata atgtcatcgt cgcagacctg aatctttatc
    18901 agtctgggcg agattgcagg ttcttccgcc agctcaataa tgacaccatt gggcagtttt
    18961 tgtactcggg aatcggcaag caaaagtgcg ccggaaatta acaccagcga tagagccagc
    19021 cttaagctaa ttttcatttg ttcgcctctt atttgtaaaa tttcaaaagc cgtatatcca
    19081 tgctccaaat ataatcaatt aatatggctg atggcaaaat tttaatcgtt ccgagaataa
    19141 tttactaatc atgttcgcgc ctgaactaaa gcaaattggc aaatgattga aaggaacttt
    19201 aacaacagag gcacgagaaa taaggctaag gaaacaagtt actggtttca agtcaacgcc
    19261 gaaatggcga gtcaagctgc gcctttagca ttttataatt ttcaccattt aaaaagattg
    19321 cctcaagggc gaggctaagg gcggtaagaa attaagtcct aataagtgct actaagtact
    19381 tttaagtgtt tataagaata tataattcgt cacaacttta taactttaaa aactcatgta
    19441 tttatgcagt catgcagtca tgcgaaataa aaaactgtat tttccgtaac ttttttcatc
    19501 cctattttct tcattggaaa atgaaaattt taaattaaaa tatacagaaa tgcgattaac
    19561 cagcctgatg aacactaaag aaattttcac ctaaaaaatc aagaaggaac cttatgagtc
    19621 tgtcatttgc cgagaacctg caaaacgcat tggaagaatt tcagaccgga actcagcggt
    19681 ttgaggaaat caaccatcac cttgcaaaaa atctcgcctg gaaaaaagaa gcctatgaac
    19741 atctaaaaaa tctattgctt cacatggtaa aaatcggcgc ttctgatata aacctcggcg
    19801 gtcccagaac caatgacaag gtttggtacc gggtttatgg aaatttagag ccgagccagg
    19861 aagtacctac ctacaccaat gacgaggttg ccgcaatcgc attaagcatt ttatccgacc
    19921 aacagaaaac gattctcttt aacgaaagaa acattgattt tgcctttgcc ttttctaccg
    19981 accaaagcca aaaaccatac cggtttcgcg gcgacattta ctacgagaga aataacctgg
    20041 caattaattt ccgacacatt gctgaaaaaa tattccctat agaaagttta gagattcccg
    20101 aaccaattat caaaaggctc gacctcaagc atgaaaaatc gggcttattc ttagttaccg
    20161 gcgtaaccgg ttcgggcaaa agcaccactc tggattctat aaccgatatg aataatcata
    20221 ataacaaagc ccatattgtc atcattggta agccgatcga atatgtccat aaatccgata
    20281 agtcattgat cactcatcgc gaagtaggcg atgacgtttt gagttttcgc aatggcatta
    20341 tcgaagcgct gaggcaggat cctaacatta tcatcgtcgg cgaaatgcgc gatccggcta
    20401 ccatatcggc agttctggaa gcgactgata gcggtcacaa gattttctcc accctccaca
    20461 ccagctcgac ggtcgaaagc atccaccgaa ttattgccga attcccgccg gacgaacaag
    20521 aaaggattag attccgcctc gctgatacat taaaagtggt aatttcgcaa aaattggtgc
    20581 ccaataaaga gggtaaaaga accctggcta aagaaattct ctccgtggat tattccgttc
    20641 aagccgccat tagaaacaat aacattaccg aaatcttcca gatgattacc gaaggtaaaa
    20701 ataaaggcat gttcacgctt caacaagatt tataccggct ggtgaggaac ggtaccatca
    20761 ccacggaaac cgccctgaac tactgtaaca acaaaaagat aatgcagcag ttactgggtt
    20821 tttcataaaa agttattgat gaatttctcc gcgctatgct ctttgcaaag aattcagcca
    20881 taagtaagaa aaaaagggta aaaaatgtat atatttggaa atcttaaagc cggctttttg
    20941 aatgcttggc tgggagtttt gctaatattc ctggcgagtt ggactatctt agccactgac
    21001 aagaaagcac gcagacgtct atcgaacact tcctggtgca gtccccggga aaaaaagtta
    21061 gtttggtccg gaacattctt attatttgta attcttgtat tctcgatatt tgttccgctt
    21121 caactcaata ccaattggtt ttacattgga acagccatat ttcttgtggg atatatttgt
    21181 ttggtgatca gctattttaa ttatatcaaa actgccggtg ataaatctaa gaccaaggga
    21241 ttttataata tctcccgaaa tcccatctat ctttttacct ctgtcgcact tctcggaatt
    21301 agtattgcct cagcctcgct cattatgctg atcttagtaa ttatccattt tatcatttcc
    21361 cattttgtaa tcttagcaga ggaacgccat tttcttcata tttacaaaaa tacttataaa
    21421 aaatatatga agagagttcg ccgctatttt ctattttttt agaatctatt catattcaat
    21481 cccggtcggg agagtttagc agatagataa attaaatatt taatcgtgat acataatctt
    21541 caactccgcc aggagttgaa cctgcttaac tcaggattnn nnnnnnnnnn nnnnnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnac cacccaaacc ccgcaggggt ttaacccatc taaaagaaac
    21721 acattcgttc attagcactt ctaccacacg gcttgcttat gttctgactt tttaagcaga
    21781 ctctacttgc ttaaaatcat cttccttagt tcagaataat tatcggcttt cattctgtat
    21841 aaataaatac cggatgaaac tggcattccc cagtcatccc ttccatccca gacaactcta
    21901 tactcgccca aatttagttt cgcatctaat agcgttctca ctttttgacc agttagatca
    21961 tacaccgtga tgttcacatt actctgcttt tccagcgaaa attttatctg ggtagtagaa
    22021 ttgaatggat taggataatt ttgagctaac ttaaatttag tttggattcc tgggtttagt
    22081 atatcactcg tattggctgt aatgatgaaa aattcaggag gcatgcctat ggtgctatct
    22141 actacaaatt ttattgtctg attgcaatta ataattctat catttttacc atcgtacaac
    22201 ttaaaactaa tttcttcgtt atgctcatca cccactatgg taaaatacca aaatccatca
    22261 ctatcatcca cgggcttttg ccaggtggct acacatctgc aatcttcttc accgccggga
    22321 ccgaatgccc cgactaagtt accgttactg ctatcaaatt tctcattatc caatttcaca
    22381 cttgctacaa gaaccatatt atattgagtg cctgtaatct gatgccaatc gggtgagtcg
    22441 gcaatatccg ttattttact taaggattta gccaaaagat tttccggata aattaaagtg
    22501 gaaggactgt ttacttttaa tttataccct tgatttggct ccatcagact gaggtcgcct
    22561 acccaagtac tgggaggatc gaagtacgtc gccgattgag tttggctttt tacttgaaaa
    22621 gcattgggca taatactctg caaagccgaa tttactgcca tagaattttg tggataatag
    22681 gcaatccaat tccatccttc gattagttcg attggagtgg atacatttat aggtgtacct
    22741 actacagaaa atttttcaac tgcctgatcc atcttgataa gataggtttt cccgtcatca
    22801 atttgggtca aatcacccat ccatttattc tgcgctcgat aaaaggtcgc ggatttattt
    22861 tgactcttta tttgaaaaat gctactggtt aaactggaaa aaactgaatc tatcagagga
    22921 ttatcagggt gaatattaaa tgatacccaa ttccaatctt tttgtagaga aaattcttgc
    22981 tttgcgaaat ttctcaattc gagcggttca tcaggagtgc cgatggtcgc attatcctcg
    23041 aaagaaatag tctggataat tgtttcgatt gcattgagtt cttccaagta aattttaaaa
    23101 ccaatcactt cgcctatttc attcccccga atagtaaaat accaatagcc atcgtaggga
    23161 ggtgaaggct cgatccaact ccctatccct cgacaatctt catcacctcc cgaaccaaaa
    23221 gccgctattt gattattatt cccgccaccg atagttttgc cgctgatttt tgctctcgct
    23281 acaaccacca tattgtaatg attgccgcta attggcgtcc aatcaatcag cgtcatatca
    23341 attttttcgg taacctggtt ttgctcaaca acaatatcct ttaatgtaac cgtggtatag
    23401 ccgtccaggc tggaggtaac ttcatatatc ccgggagaaa tagctagatg ataattgccc
    23461 tcagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccatcg caatcataga
    23581 atattgcgtg ccagtaatcg gttgccagtt cggcggattg gcaagaatga tcagtggaag
    23641 catcagcatt aataggaaaa cggcgagtct gcgcctcacc tgagcatagc taaaagcttg
    23701 cgcaaattga aatttatttt tacttaacat attcttgtcc tcctaaatcc gtgtaaacgg
    23761 tattcttagg atttattaaa cattcaagga cagaattaat ccagtaatca caaatcccta
    23821 cttccttatt gtaaagattc ttagatccta tttaattaat aaacatctct ttattaggga
    23881 ctgaccaccg tactggattt tgtagaagta tattcctgaa cttaaatctc cggcattcca
    23941 ttccacggta taattaccag aatcctggaa tttatcaacg agggttttta ccagattacc
    24001 ttttaagtca tatattttca aattgacaat acccggctcg ggtatcgtgt agctaattgc
    24061 ggtaaccgga ttaaagggat tcgggtaatt ttgttcgagc gttaatttcg taggcagggt
    24121 tagagaaacg tctgtcgctt cagagccact aaatttgaca actaacggct cactgggtgc
    24181 gccaactaaa gcatcggaaa cgaattgaat tttttggtca gcggttaagg cacattcttt
    24241 cttcgcatcc caaatcttaa aattaagcga ttctccgtcc tcattgccga caatagtgaa
    24301 ataccagcag ttttcctctt tcttcccgac agaccgacaa ttaccgcggc tatcaaaaac
    24361 cccaattgta tatctctcgg aatcagttat tttttcagca tcaatctccg gctttaccag
    24421 tgcgatcata ttgctcttat tgcccgacat tactttccaa ccggctaaat tagatgcaag
    24481 ttctttttga gtcaacggct tagatagggc accggagaca aaaggataag ttagataggt
    24541 tacactatca tccgttgtta tttttaattt ataaccaatt ccgggcgcca ttttggtaag
    24601 gtcaccgatc catgtattgc cgatacaaat agccgattga ctctggtttt taacgattat
    24661 ggtatcgcca ccaattatac tcgccagcgc ggtattgagc ggtgattcat tctgcgggag
    24721 atatccaatc cagttccaat tttggtctag agaaatcgga tgaattaccg gattaataag
    24781 ttgtccctga aacgtaaaag aattaaatgc atcggtcatt tttatcaagt agccgtcact
    24841 attgttgaca gtggttagat cacctaccaa attcccactc ttacttgctg tcgccgaact
    24901 attttgagtt tttacctgat aaattaaaga tgcatcggta aggtctttaa ataccgaatc
    24961 aatggatgct tcttttggtg ataaattgaa agatacccaa ttccatcctt taactaaatc
    25021 gaatttctgc tccttactgc ttccatcaat agtcaaaacg gtatccataa tctggcaatc
    25081 tgcaaaaata atcttttcgt tgcaatcata aacgtcatcg gtctcggtat cataaatctt
    25141 aaaactaatc gtatcatttg tgttattatc actaacaatc gtcaaatacc aatagcctgc
    25201 aatatcccag gagttatccc aataaggatg attaccttct ttccaagttg ctattccccg
    25261 acagtcatta gtgccgccgc tcccgaaggc ggctaattgg tcacttttgg ttttggtgaa
    25321 aaaatcacct tccaaagata cagtcgtaaa taggatcata ttgaattcag ttccggtaat
    25381 tttactccag ttaatcagcc gcatatcaat attcggagtg acattattgg ctaccacctg
    25441 aacattattg atcgtcaccg gagcatagcc tgccagggaa gccgtgacat cgtaaacgcc
    25501 ctgatttaca gtaacctcat agtcacccgc ggcattaacc ttatccgtaa aatgatacgt
    25561 tttttcatcg tttgaaaccg ttattactac attcgttaat gagccaccgg ctactttaag
    25621 ggaaaccttc ccgctgattg tgccttcaac gactacatat ttcagactga aattgactgt
    25681 cgtgggagca cctgaggtta cagcaattgt atctacggcg gtttcgtatc cggttaacgc
    25741 ggctgtgact ctataggtac cggccggtaa atcatnnnnn nnnnnnnnnn nnnnnnnnnn
    25801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnncttcc attagataac tgcagaaaat atttgccgtc tttatcaggc
    25921 ttggtggtag atgaagtcga aggagccgta gtcagggttg cttttataac cgcatcttct
    25981 accttcccgc cgccaccatt aatagtaatc gttccctgaa tcccggtgcg aacgaaataa
    26041 tatgctccca tatctaacct ggtcccgtct gggtcctgat catctacatc atccgtccag
    26101 gaggtgccat cagcatcgag gtcaggattg ccagcatcaa tagctgggga attgtaatta
    26161 agagtaaaat cattgttatt gtaatcttta aaaagcgggt ctgtagcaat acatccttca
    26221 ccaaagtaac tttttccggt accattttta atatccgaat aagtcgcgga gatactccca
    26281 gttccttcaa tataaattgg atcgggtaga tattcactcc agataatcga gtttacgatc
    26341 gtaatctttg aggcatattg acaggcaata tcgttaccat tcgatttggc ggtgtttttg
    26401 gcaatagtaa cattacgcaa gaccgggtta gaattattga aacagcagat accgccgcca
    26461 gttccaccgg attcgttggc tttattatta tagataacgg ttctttccaa agttgggctg
    26521 gacgaatagc aatggattcc cgcgccacta tatttggcta cattatcgcg gatgaccaca
    26581 ttttttatcg tagggctgga attataacaa taaataccac cgccatagtc agcacttcca
    26641 ttggtgacag tgaacccaat caatactgaa cttgaatctt cgccaacact aaatttaacc
    26701 acagttccag tagcttggcc atctatgatt gttttttgga tgtggtcagt tttgccatca
    26761 ttataaaaaa gtgaggcgac tgtgatcttc ttgcctttga agtcaatctt ctccttataa
    26821 gtgccctcag ctaccaatac aacatctccg gttactgccg catctatccc agcctgaata
    26881 gtcggctgat tactcggaac gtttataacg gctccaaata ttagtccgga aaataccaaa
    26941 agaattagac tagcgctaaa aaatttcttt gccattcttc acctccataa ttttttataa
    27001 tataaacctt tttttaaaaa taaatagctt tttctaacaa aatatttacg ccccaattga
    27061 aggtttttat aagattcagc atggaaccgt taatacaagg aactcctgtt atctaatctc
    27121 aatacttcgg ggttgagatt agtcctaaat actgtcggca aaaatatatc tgctatcatt
    27181 ttatctttta aggggcaatt tgaatgattt tcaatccttc actatcggta acgaaggcgt
    27241 aactgccatc tacaaaaacc gaattgggag tggttgacat ttcaaaacta cctaagataa
    27301 aaggattagc caaatccgaa atattaacca cgattaagcc tttttcctta tcggctaaat
    27361 aagcataatt gccacgcacg tagatactat aagcaaaacc gtgggtggga cagtttgaca
    27421 ccaaggacgg tgcttctaca ctcgatacat caactatcac taatccattt tcaccgtcag
    27481 caagaaacgc ataattatct ttaataaaca catcttgagt aatactcgag ctgggaaaat
    27541 ccgcaacata tatcggattc ttaacatcgg aaacatcaaa tattgtcaag cctcgcgaac
    27601 cgtaagccac gtaagcataa tcattagcta caaacaattt tttggctttt tcatcagccg
    27661 aggtattggg aggtgtaata tttgaaatta tatgtggtgt actactcaga attgaagcga
    27721 tggacacacc tttctcttct tcgcaaagat aagcataagg tggttcaccc gttacaaaaa
    27781 catcaacagg gactccactt gtctggcaat taccgattaa ttccagcgta gtaacatcaa
    27841 caatattcaa gccaccagtg tgattagtag cataggctaa atttttatag atataaattg
    27901 acaaggtgcg atcgggtaaa ggtaaaacca aattattatc ggtagagggt gaagatggat
    27961 ttatcaaatc tataactatt aaaccattgt aatgattagc aacataggct ttccaatccg
    28021 agacaaatac ttcagtggca gtaccttcag tggttaggga actgactaag tttaaccgta
    28081 tgttaatatt tccctcatca gtaggttctg aaaaatattg atttttaaat gctcttaccc
    28141 gataataaaa attcccgcat ggttctgaga tattatccaa taatgttgtt tggttgctat
    28201 ctaccgaagc atatccattt acccaatcgg acattccaac ttttcgatcg atataaaaac
    28261 cggattcacc ttgactatta tcctgccagc taattttcac aatatcctca tcaattttcg
    28321 ttaaggttaa attagaaggc gccggaaagg tattgtctat ttcatcggta gtaacatagc
    28381 tggaaatata ctcttctgaa aatccatata cccggtactg gagtatctca ttgatttcgg
    28441 cattctcatc aatccaccag gtctgatttg gatcgagcgt tgctatctca acctgccaat
    28501 ctgcactacc gaccttttta tctattttaa agcctgcgac attggatgtt ttactttgcc
    28561 aggttagttt gattttatct atagcaattc gttcatattc aagattatag ggaggcggaa
    28621 acgtagaaat gccaaaacct tccgcactgc ccgataccga tatgccgcag aaggcataga
    28681 ctcgataaaa aaccgaatcg ccgaaggaag ttttatcggt aaaggaagtc acatttctgc
    28741 ctaaggtttt ataatcttta atccaattcc cgccgctaac tttcttctca attttataac
    28801 catcttcgcc aatacagtta tcccgccaag tcacctcaac cttatcagat tcgaggaccg
    28861 aaatagctat ttccgtagga atcgtttttg gtgaaaaata ggctactacg tcggaaaatt
    28921 ccgatgccaa ttccgatgta tcactttcgg tcttgatctt ataggcatag atcaggctat
    28981 cattagttgg aatttcatct atcaaaaaat tatcataagt ctgagcatat tcaagattcc
    29041 aattagcatc tcctactttt ctggcaacgg taaaaaaaac gtcctggtca gattggtcat
    29101 aatcccagct gacttcgatg ttattatcgt cgatagtctt gattctggga ttttcgggaa
    29161 ttccgatccc accgggtgaa gtaattttgc cacagtcgaa aatgaccact aatgaaatcg
    29221 ctaaaaaaag aaatagtcct gtaattttta atttattctt catccaatct ccccaatctc
    29281 agataattga cactccccaa ttaaattatt gccaatagta attattcatt cttgcacttt
    29341 tttgaacagc agggtatcaa tatgttcttt tggtttatag tatttctcga ttaattctct
    29401 agctgattct ttcttcgcca ttcttacttc gataggttca atgagctctg cttttaggat
    29461 aattataatt tctctttcat ttttctcgtg attactgtaa ccaaatatat accgcaatcc
    29521 caaaacccac cagggcaggt cttttaatat tggaactcct ttgcgaacaa caatatcttc
    29581 cgtgtcataa agcccggcaa ctacggtttc ttctccatca tataaaatag cttcggtagt
    29641 ggcttgattt ttattaacga ttacttgcac atccgaaggt acaacactac ttttttcaac
    29701 tctggcaacc aaatgtattg cttctttact gctatcttga ataatcgtcg gagttacctc
    29761 cagtataagc ccggcggaat aaaaccgatc aactaggttg ccagaaaaat cggtggttcg
    29821 cacggaaaaa tcttgaccaa tctgcaaata tccttgcttc ccactaatta ccgtgacact
    29881 aggtcgggcg ataatggtac ctttattata gtgttctatc atcttgaaga gtgcatttaa
    29941 ttcaataata gcttctcccg attctaaggt agtggacacc gttcccgaaa ttatatcact
    30001 aagcacttcc gacccacccg taaaatccag cgaggcaacg acagtgccgt tataaatcgt
    30061 tgaccaatca ataccaaggc tattgataat tgacctgtcg gcagtaaaaa atatcgagct
    30121 gattctaact tgtttggtat cgggaacgac ctcatcggcc ggtttttttt cttgaaccgc
    30181 tttctcttcc gctataaaat cctttaccac atacgctccc gggagttctt ccactactaa
    30241 cctgttataa ttggcaatta aatttaaggc gtttttccag taaatctgag taataggaat
    30301 gctaatcggt ccggtatatg tgctcatatt cacgatctta cgcccttcgt atttctgtga
    30361 tagcgcttcg atcattttga gagcatcggc taccagtact tcgggtgaaa tagtaatcag
    30421 ttcatcacta gaaggacttt taatgagatt tacctgagag ttagttgaac tgaaaaacaa
    30481 caatagacaa ataaataatg ctttcttttt catatcttat cctttcccct gcaggtagat
    30541 agttttttct acataaacac caattctatt tattttaaag gtaactgctt ggttttccca
    30601 atcgattttt gataaatagc cataagctac tttttcgcca gttgtcaggc ttatggtgcg
    30661 gccgttctta tccttaaaaa aggcaatagt tggagttaat cctactagga ttgcttcttc
    30721 tggatttaaa tactgttctt cttcctttat ttgcactggg tcatggatcc cgggataaaa
    30781 aggattaaac ctaatgttga ccgttttaat ctctcttatc ggaatacttt caggctcaat
    30841 cccgttctca tcataaaatg cacgtaattt gaatgcgaag ggaattatat cctcactagc
    30901 tggctgttct cttatttcta ataactccaa cagataaagt ttgggttgat tctctatttg
    30961 atagataaaa gtatataaag aattaaaggg aacaatccca gcaattaaat aatcattata
    31021 ctttgttcct tgaatctcgc cactttcggc cggtttgaaa tcaaaaacca tttccggaca
    31081 aaatttctgg catatctgcg taagataatc gtaagtaatc gtcggattat cctctttcac
    31141 aatggctttt ttaaatttag atatttcgat ttctaattcc ctgatttctt gctctagttc
    31201 ctcttcttca ccaatatttt ccagttggct attgagcata ttcaggcgtt ttgaaagttc
    31261 ctgatttttc ttacttaaat cggcaatttt tctctctgag ctggaatttg aaatgacact
    31321 tcctattagt actatcagta agatcactgc caaggctatt gtatttctta ttttataggg
    31381 catattaatg ttcggttacg tattttattt tttcctcagc catttttgct aatttatgtt
    31441 ctggatactt tgtgattagc attccccaac agtcaatggc tttggcttta tcaccgagtg
    31501 cgttgtaaca tctccccaac atcaacaggg catccggagt tttagcaccg tcctggttag
    31561 caattttttc aaaaatatct ttcgcctgag ctacttcacc catcaggaac agacattcgc
    31621 caattaaata attagcacta taagtaaagg gactattcgg atatttctct acaaactggg
    31681 tgagcttgtg ataaccttcc tgtatattac ccgatagata ggtctccgcc gcttcgcgga
    31741 aaacagtata aggagtaatc cttggggtag attgatctgg tggtgactga ctttgaacaa
    31801 cggcaggcgc aggtgcggct aatttgggag cgctttcact ctcaattgat tttggcgctg
    31861 gagttatggg aggcggagcg gcggtttttc cggattgcgg agtaactgga gctggtttag
    31921 cgactttcat tacccattcc tcctgatcag agtacgaaaa attcatcaca aatcgcatca
    31981 ccggtataga ttggatctcc gaggttttga catcggaaac tatacatcct ggaaaatacc
    32041 tggtaatata ggcgatattc tctttatggt tggtagtgcc ctggaccata aaaccgctag
    32101 tatcactcct aagataatta atccagctaa tagagttttt agcgaaggca tctgataacg
    32161 ctattatggt acgatgccac tgactcttat tgcctgaaat agtcttaatc ttatccatac
    32221 tttttcgctt accggtaatc tgctgagaga gttcattagc tcgggcgaac ataagattct
    32281 tgtgtcttaa ctcggactca atattataat tctggcgttg aagttctaca atctggcggt
    32341 tattttccag attttttagg gtagcggaat aggtgaaata aaatattgca ataaacagga
    32401 taaagccgtg ccagccgatt ttaaatcgtt tttgactctc gataattttg ggcggtagaa
    32461 gattagtatt atagaaagct ttatttttag gatcggcggc ttttaatgcc aatgagattg
    32521 gtatggcata ttttgcgatg ttttccggag aatattttcg gtttatagta gcgatattga
    32581 tatttttaaa ttcgaaccgg ctaacctcgg tcgtttcgcc gaatttacta ctgaaaaacc
    32641 gtatatcttc atctcggacc atattaccgg tcaaaatcaa atgctgaacc ggtggaatat
    32701 tggaaacgtc ctgctcaagg ataatcttgg aataaatcac ctgtcggatg ttttccgacc
    32761 tagaactggt atcgccgata atcttaaagg ttttaacgtg ggtattattt cgcatcacta
    32821 tgcccgcttt atactcggaa cccacataca agatcaagac gtagtcgttc tcggggaaat
    32881 tatagttagc tctaatcaaa ttcatcaagg caatttcatt ggtatcaata taactaaaaa
    32941 tgtatctctt tggggataat agcggtctta cctggagaat tgcattaagt aaatcgaatt
    33001 cacctcgatg taaaaatgcc agagtcgtac cgtcataatt cggtatgcat ccaatggcat
    33061 atttcctgga tttcaattct tcctggctta aaatttcggt ccttattctc ttcttaattg
    33121 ccgataaggt taacgacgaa ttgaagatat ggtaagatat ttgttcctca ttcgcattta
    33181 gtgctatctt tccagtttct aatgaaaaat tgcttaaaaa tcgctgcagt tccgaagtag
    33241 aagtcccccc ttcttcgtta acttcggtta tctcttccgt gagtttattg ctttcaaatt
    33301 catcgaattc cgagatttcc ggtaaatcaa atccttcctc tttcagatca gggttatttt
    33361 gtatttccag gttggtatcg cttaattcaa tcgtggggat cttatttacc tcttttttat
    33421 tatagagagg ttcagataaa gtcgtttcgc ctaaattttg gattttaatt aaatcttcat
    33481 cccttacgag atgagctatt tgtatatcta atccatcctg gaaaatacca tagtaatttt
    33541 tcacttttta ctcatttatg aagctcaata aggttataca caaatttccc gataggcaag
    33601 caactgctct attctttttc tattattgga gaagtcgaga gcagtttctc tacttataat
    33661 ttttttagta tatagattat gaaggtcttg ttccatcgta accattccgt actttttacc
    33721 ttcggtaatc atctgataga tttcgccaat atttttgttc ctaattgcgg cttttactga
    33781 atcagtgaca gagagaattt ctttcactag ggttaatttg ttatctctat caggaactaa
    33841 tttctgcgaa attactacgc taagggtatc agccaacctg agtctgatgc gctcttgttc
    33901 ctcgggtaaa aattcggcta taattcgatg aatactttca acggctgaac tggtgtgcaa
    33961 agtggtaaat actttatgac cggaatcagt tgcttccaga accgtagcaa tcgtaggcgc
    34021 atcacgcatc tcgcccacga taattatatc ggggtcttgg cgtagagcct ggatcgtgcc
    34081 ttccctaaag cttagaacat caccgcctac ctcccggtgg cggatgatac attttttgga
    34141 tttatggata aactcaaggg gatcgctaat aattacgata tggccttcat tttcttcatt
    34201 gttcaaattt atgatggaat cgagagtagt gcttttccct gaacccgtta tgccagttat
    34261 taaaattaaa ccggatttct catgccgcaa attaagcctt ttcaaaaccg gctcgggaat
    34321 attcaaattc tctatcggaa aaagttcgcg attcactctt ctaaagttgc ccaccaaaac
    34381 gtcattatcg taatagatgt cacctctaaa gcggttggct tcttcttttt caccaaaact
    34441 tattcctagt gaaaaatcaa tattcttttt atcgaaaaga ataattttct ggtcatcgga
    34501 caatatactg agcaaaatcg ccgtgatctc atcattggaa taacgcttag cttccaaatc
    34561 gggatatttc ctgccatata ctctatacca gattttcaca tccaacatag aaccgccaat
    34621 gtctatatcg gaagcgtcta tctcttccat cttttttaat aagcttttta aattatcata
    34681 agcttctttt ttccaggcgt tgttgaatgt aattaactta ttaatatttt taaatctggc
    34741 tgtaccaatt agatactccg gtaaggctat ttccatttca ggtgtaaaag ataatcccat
    34801 ttttacctca tagacttacg taaatcggtc aattttaata tcctttattt aagttcatac
    34861 attatatcaa aatggtagat gagtaaattc aacctttatt cactctctgt ggttaccagc
    34921 ttcagcataa taattctctg aattgatttc ctccacttga tatataagca attgattagg
    34981 ttaatcataa attttttgag caacttttct cccttgaaag caaagaattc gagtaatttt
    35041 ttacgctcaa aaaagcgggc tgttaccggg gtttccgctc tcaagacact ggaagtaggg
    35101 ctattaataa cgaaactctc ttcgttccag gtatcccatt tcttcagata atctactgag
    35161 gtattactta accaaactga aacttcgcca ctttcaatca aaattattga actagccatt
    35221 ttatcggcga ttcttatatt ctccccaggt tcaaaattgt aaattgagct gagtatcata
    35281 aaatcttgct tgtcctcctc ggagaatcca cttaaaatag ccatttcacc tttttctact
    35341 agctcggtcc ccggttcgaa tattttcagg agaggcccat ataaatcctg cgagtatttt
    35401 ataccctcct tgaccgcatt aagcagttca tcaatattga ttggttttga taaatactgg
    35461 aatgcaccgg catggacggc tttaatagcc ccttcgatac tggaaaatcc ggttagaatc
    35521 attacaaccg ccaggggctg tttttgcttt atcttcttca tcagctccaa tccatccatc
    35581 cgcggcataa ccatatctac cagatataaa tcaaaatcat catagtccat tttttccagc
    35641 gcttcctcgc catcagcggc taaggtaact ttatatccgg cttcctcgag gatttccttg
    35701 cagagcattc taatagtttt atcatcgtca acgacaagaa tatgactttg cataattttc
    35761 tattctcgct tctctctttt ttctttaatt aaatctaatt ttttgagatt ttgtaatcta
    35821 ctcaagatgc gttttatatt atcaagtgct tctgaaacct gttgactgct ccgcaataat
    35881 ttctcgtcat tatactgttg cgccgcattt tgcaatcggc gttctaccag ggcaaaaacc
    35941 atcaattggt tattgatgtc atggtcaata ctcgcgatgg aagcggctac ttcaaccact
    36001 tcctcatact tacgaatcaa ctcttcctga tcactcatat aatttttccc ttattttatt
    36061 cttgcatcat tattccaatt acaacactca ccttatagtg taaagcagtg aagaatcaaa
    36121 ttagaaaaac aacctccgtc aatttctgtt tggcttctga ccaaaatcat ctgtttccga
    36181 taaaacgaca tcagcgaata aacttacatt ttaacgttca aaactgctct caagccattc
    36241 tcatttcgac tcaatcccac cgtaaccttt agtcttgatt aatgaccccc tgtgaaatac
    36301 ttgcttcgct atgctgcaaa ttttacgggg cataccaatg actaagagcc atttggggta
    36361 cccgagatgg ctttctacta cttaattttc aaaaaacatc atatctctta acgacagtgt
    36421 ccgatagttc tcattgaatc caataggcta atctcaaatc ctttattcct ccgagactaa
    36481 caagatcttg aatatcgtca tcatccagag cttcattcag aatcttgacc tcatccagct
    36541 tagtatcggt acaatttaat ctattaataa cctcatttag taaattggct aaaaaaccag
    36601 tataagtact ctgattaata tcaccaccaa cagtaacata gctctcatcg gtgcgtccct
    36661 gccatttcgt agttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttttac
    36781 gagctcttac actccatata ggattatggt cactattact ctttttctca gctgccagaa
    36841 ttccccaatc cgtattagta ttggaaaaat ttacccatgt cccgacggta agttcatcgc
    36901 catccggctc aattaagttt tctcctacat acacataatc attatcacca tcaaaatcaa
    36961 gcgctgagcc atttttgcca acggttctgg taggtttata attggtaaca ccattgccta
    37021 aaattccatt attgtcattt tttgagccat caaaaaccga gtctggatta ctttcatcaa
    37081 aatcaaaacc gaatatttct gatttaggat gttcctctat ttcgaatctc accatcgctt
    37141 cggtctcaaa agcctttact cctttcccga tgtgctgagt aataaaagac gatatccgaa
    37201 tactatctcc agccggatta agagcagtat aacgaatgct atcaattgta ccaccaaaaa
    37261 aatcagataa cttatcactg aaatctacgt ctaatctttc tcttaaaaat cctgccagat
    37321 gccaatcttc atcgtcatcc aattcgtatg cctttttcaa cgaatatgtc agcgcaaaat
    37381 cgcgcagatt tttagaattc ataaaatcta tactctcggt tatctggtca ggaatattac
    37441 taactcgctt ttgcactcct accgtaatcg cggtgaaggt cacggtaaga ataattactc
    37501 caattaaaat tgccctgccc attttaatac ctcctcataa gttttttaca atttatttcc
    37561 atcataaata aaaatttata aaatatttcc aaaaagtcaa attggttata atattccttt
    37621 cgccagggcc aacataactg gtttcaaacg caaattgcag atcagttttt aataaacgga
    37681 tgtaactcaa cgaatctccc ggagtagtaa tttgattctc atcaatatca tagtaggaaa
    37741 aattcaaatc tctcagccat aatggaacat ttgagccggg caaaggcacc ccattttgca
    37801 ttattttaat cggaatcccc tggctggtgc tatcgcctaa tgaaatgcta atggtattaa
    37861 ttaccgaacc cattatatca ttgattaaat catacttagt tttgaataca aaactgtccg
    37921 gctcggcgac taaaatttgt ccgctataaa cccctgctcc tacttttccc aggcaattat
    37981 cgagctcctg atattgaact tctgccgcgt tgtttagcgt gacgtggaaa gtggtgtctt
    38041 gcgaagatgc ctgcaaatta aacagggtgg taaagatcgt tatcaaaaat aaaccgccga
    38101 tacttgtcgc aaatattgca tctaataaac tggtcatact aattctatat tataataaat
    38161 attattaaac tttagagtat caaggattac aaaaggatta gtccagattt ttacatctac
    38221 tctctggaaa tcggttttgc cggcggcatc caccccgctg gaatcacaat agtggctact
    38281 aacattataa atataccaga cgtcgttacc ccagattcgc agtgaatccg tgccggaata
    38341 agtgatggca atatcgtcaa aatcaatcaa atccgtcttt gtttcgacag ttattttatc
    38401 aaagacttta ctggctaatt ttaccgcctg attcatatag attgattgat aaacaatttg
    38461 tctttgattg ttgattgagg tattcatcgt taataccacc gacgtaaaaa gtgcaatcga
    38521 acctatcaga gcaaacattt gaggataacc catctctaac cctccgatgc ggcataagaa
    38581 acttccgaaa cagtggttac tccctctctg atcttttcta atcctgaatc aaggatcgag
    38641 agcataccct gcccctcggc aatttctcta atttcagctt cgtccacttc agctcccgaa
    38701 tttaagattg actttcgaac ttcagggctg aaatagagtg cttcggctaa actaattcga
    38761 cctttgtagc cgccattgca tttcttacat ccggtaccgg cgtcaaagat tttgcccgat
    38821 ttcaggtctt cttctgttaa acccaattct agcgcggctg ggtacttttc tgaagatata
    38881 ggtacgcggc aaaaaggaca gagcttgcgc actaatctct gagccacgat aatgttaatg
    38941 gcgtaagcca gcaaaaacgg ttcgacaccc attttatata agcgcgagat agcgctggga
    39001 gcatcattcg tatgcagggt agtaaaagtc aaatgaccgg tattggcaag tttaaccgct
    39061 acgtccgccg taattttgtc tctaatttca cctaccatca caatatctgg atcgtgccgc
    39121 agaatcgttc tgatagcatc ttcaaaactt aattttctac caatctttaa ctgccttgct
    39181 cctttgatag catactctac cgggtcttcg atggtgagaa tgtttttcga cgcatccata
    39241 acatgatgca gggctgccag aagagtcgta ctcttaccgc taccggttgg tccggtaagg
    39301 attactatcc ctttagattt acttattgct tttataaaat cttgttcagc ctggggtaaa
    39361 aatcccaact gccttaaatc ggcaattaca tttctatcat ctataatccg gataacaatg
    39421 ctctcgaaac gcctttcata ttcttcagaa atgatgggca aaaccgaaac ccgatagcga
    39481 attaaatagt catctacatt cctctgaaaa aaaccatcct gagcggtatc tctttcaaaa
    39541 cgatcaaccc caatagttcg atctttgact accgccgcca tcgcctccgg cgaaacatta
    39601 tcctgcctat accataactg taatcttcca tcaattctaa aataaatgtc aacgctgttc
    39661 ttttcataag gaactatatg aatatcactg gctctcctcc gcacggcttc gatcaaacaa
    39721 ccttcataaa gattaaccag aagactttta ttgatttcct catccagaga atattcgtct
    39781 aattcagtaa cttgtttctc ttcgcttatt tccggctcga ctactgcctc cctcaaaagg
    39841 tctaaaaatt cattttctcg cggcgatatt aggtcaatca attcctcaac tattttgaga
    39901 ggagtataaa caacttcata ttttttaaat tcggtgaaaa gcggaatttt ttcaacgacc
    39961 ttctccgtcg gattcgaagc taatactaat aatttattcc tggaaccatt gattacttga
    40021 taaggcagaa ctttgtaata tagcagttga ttttttagct catccggaaa tttggataat
    40081 aattccttag tgtgctttat ttgagccgga tcgagcttct ccggatttac tcggtaagtt
    40141 cggaaagcgt ataattctgc cagcgtacca aacaccaaat catgatcaat tttgaaattt
    40201 gagaccagta tatcttcaag tcttttcgaa ctagaagcat ccccctcatt ctggatttta
    40261 agggcttgtt ccagaatttc tgagctgatt atgcc
//
LOCUS       DAOMJU010000009        39947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_830_length_39947_cov_18.630979, whole genome shotgun sequence.
ACCESSION   DAOMJU010000009 DAOMJU010000000
VERSION     DAOMJU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 39947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39947
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_830_length_39947_cov_18.630979"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="PLO58_01675"
     CDS             complement(<1..1409)
                     /locus_tag="PLO58_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73530.1"
                     /translation="MKKFIGFLAFLFGSFSILLGVPGEYTFKVFDLPGGWNGTISFNA
                     VANSNVYDCNHDPTTLYHSAQTTTAVGGSWAAFDWFNDNSGYPLLAYGEYTITVEYPG
                     KSAWFTFSLLDCYYSECGIDPPIPFTSYPMDMYFHYDFDDDKFYRASGGLVPISNGST
                     VGIWLNQVGGDPPGPCTDCFNPKVYLKNEYNGIENFGRLVVDENDNVESGHFINLPLN
                     TSHDVRSEFETYTVSSNYTQQHGWNDSEDDIVYKKSIQVVDWSLVKKSNFEKAIQSEI
                     RTNYLFGGNCNIQFEFKDPWKVEASYSRPCAYRSYSSGQSFTVFENENSGFNYQYPIY
                     SLRVPQEVDDGNLIAVFYNWTINPSGGAVLSSSGPETDVVFVSDGVTVTANYYHWYRS
                     AFQGSIPIDLTLNGNINMVGDVTVAETGNLVVQPSTVFKIAQNKIIRVYGDFTASGSG
                     IVFKPVDPNYTIKNYWKGI"
     gene            complement(1424..2722)
                     /locus_tag="PLO58_01680"
     CDS             complement(1424..2722)
                     /locus_tag="PLO58_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73531.1"
                     /translation="MSSDNNLKVKTVMSIILLISILLCAMSCRECPTEPEEYAIDISA
                     DFVGASRVRLLVSVSDSGDVREFGIKRNGEVISKSQIAGKDTLITDITAEPATAYQYQ
                     GVVYYDRKEINASEVITLTTLDTTSHEVDWKIQKIGWLSSYLRDVFIISEDDIWAVGF
                     IQDLDSAGNYITYNATHWDGVEWKPQRIETLYDNRIIFEKTTSCVFAFSYDDIWMADY
                     PKHWNGTSWQLFHLHDMGIDAYPIKRCWGASSENIYFVGNNGFCIRYNGENWTIIPTG
                     TSLRITDIWGTAADHIFMVGSDESTGKNVFIEQRNDQIKTFEFDTRLKKGVFATAPND
                     VYIVGAGLFYYDGYGVSEIPWPEDIPKNFLESVSGNGSNDLFAAGHLQIIIHYNGKEW
                     YSYHESNPDIVYYDISVKEDLIVAVGTNGPESIMLMGKRE"
     gene            complement(2793..3212)
                     /gene="nuoB"
                     /locus_tag="PLO58_01685"
     CDS             complement(2793..3212)
                     /gene="nuoB"
                     /locus_tag="PLO58_01685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HQO73532.1"
                     /translation="MGWYKRRLSKSLWVFHVSGSPCNNCDIEILDLLTPRYDVERFGI
                     KLVGSVRHADVLLVTGVFNKKVAPKIKKLYEQAPKPIFVVAVGTCVSGGCCFKDSYNI
                     VPSREAVIPIDLYIPGCPPKPEAMIAAVAKLIGVLNG"
     gene            complement(3214..4143)
                     /locus_tag="PLO58_01690"
     CDS             complement(3214..4143)
                     /locus_tag="PLO58_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQO73533.1"
                     /translation="MTGWVFFYLLVFPGLLFSAVLGLLVGWVDRKVSARVQFRVGPPL
                     FQNFNDFFKLLGKETIIVKESLHSLFVLSPLVAFGVTTLITTMLGVALFYGIGFGGDL
                     LVIMYLLMIYSVMVILGGSATGNVYSSLGAGREIKLLLADELAFILVCLVPIIKSGYH
                     IKLEEILSAQVAEGMFIGSFSGALAFIVGLLCIQAKMTLPPFHIPEAETEIVEGPLME
                     YSGPLLGFWKLNQAMQWVAFPFLLILLFLGGFSEIGWGALWSVLKYLGIVVVMVLIKN
                     TNPRVRIDTALKFFWKYLTPVALLAVILAVLGV"
     gene            complement(4140..4643)
                     /locus_tag="PLO58_01695"
     CDS             complement(4140..4643)
                     /locus_tag="PLO58_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQO73534.1"
                     /translation="MKRLFIDIPMLYEAGDAVQKIECEYFYHRRNNGQFSLLEIAEFA
                     VYCRQCQDAFCVTVCPKEALERTESGLIKRWNMRCVGCKSCTLACPFGTIFPEVINYV
                     TGKCDFCLNQLQDNPEYVPLCAKTAPLNSIRMEEIIQEEPSAQIFLYGEHLAIKSPSW
                     RHKEEKV"
     gene            complement(4640..5464)
                     /locus_tag="PLO58_01700"
     CDS             complement(4640..5464)
                     /locus_tag="PLO58_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HQO73535.1"
                     /translation="MSKNPYETTAAVIYEIIEESPTIRTLRLRPEKPMPFLTGQFMEV
                     TVPGIGEGPFTPSSSHYIADTLDFTIMKTGFVTEYIHRTKVGDRLGLRGPYGKGYPLA
                     EFKDKDVLIMGGGVGMAPTRSLFLTLIHDIDDYNSVTFLAGARTPADMIYKDQISEWR
                     KIPKVTFLRSVDKVPEGQSWDEEVCLVTKLLSKISIDPHNNPVIVCGPPVMMKFGTFD
                     LLKYGYPPQNIYLSMEKKMYCGIGQCRHCVMGPYFACKDGPVFTYEQIKDEENIWE"
     gene            complement(5457..6503)
                     /locus_tag="PLO58_01705"
     CDS             complement(5457..6503)
                     /locus_tag="PLO58_01705"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQO73536.1"
                     /translation="MIIEKAALFDFLTRCQKENLGDFKQVIAPRRSDKSDDVLFFDNW
                     REGAEFVIDSYRTIDPLRMLFYMARERILPAMTSDEKWLIVGVKACDLQALEVLDKAL
                     INADFVDPLYEDRRKNLTIISADCNEIAPTCHCNLVNGRPYATKNFDLNLTRLDTKYY
                     HLEVGSEKGQELLDLMQKNVKISRNLEDDLNTRDENRKAIEKRLILQNKQYERSQQYE
                     KLKATDLTKWVDEAFTCCGCGGCTNICPTCYCLILNDETSGEIFTKDRSTDTCQLHGY
                     ARVAGGATPRPKMFERFRNRYLCKFSYMQSNFGLIGCTGCGRCIEVCAGKIEFREVVK
                     RIMSFENVREAANV"
     gene            complement(6558..6926)
                     /locus_tag="PLO58_01710"
     CDS             complement(6558..6926)
                     /locus_tag="PLO58_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQO73537.1"
                     /translation="MKVFIADDSEILRKHLTNLLTEIPGVEIVGYGEDTESAISGIDT
                     QKPDVVILDIRMPGAGGIYVLKTVRQNRPDLRIIVFTDYPYPQYRKKCLEEGTDYFFD
                     KSSESEKLIELVRKMAGGKA"
     gene            complement(7146..7589)
                     /locus_tag="PLO58_01715"
     CDS             complement(7146..7589)
                     /locus_tag="PLO58_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQO73538.1"
                     /translation="MAITRWTPRSSWLDVRNEFDRLFESIFNPQYEEETSLLAFSPAV
                     DIIEEDKAYNIQVELPGMNKDDIKIAVKDNYLTISGEKKRESKVEDKNYQRTERIYGS
                     FQRNFRLDDSVEADKIEAEFKDGVLNIVIPKKKESLAKQIEVKVK"
     gene            complement(7626..8057)
                     /locus_tag="PLO58_01720"
     CDS             complement(7626..8057)
                     /locus_tag="PLO58_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQO73539.1"
                     /translation="MAITKATPKSVTRKSPYTIDRFFDPFFDWDTETSLVTFAPEVDI
                     EEKDTEYVVTVELPGVKKDDVKISLKDNVLTISGEKKRVKKINKKNFYQCERVFGSFE
                     RSFRLPELVDHEKIEAEFKDGILTIVVPKSPESKPKSIEIK"
     gene            8391..8768
                     /gene="folB"
                     /locus_tag="PLO58_01725"
     CDS             8391..8768
                     /gene="folB"
                     /locus_tag="PLO58_01725"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQO73540.1"
                     /translation="MGIVRLNNMVFYGYHGVAEPEKILGGKIEVDVELEFDMTKVITT
                     DHLADTIDYEKVYQLVQGVVTQSKFFLIEALAGKILSEIFNHHLVESVRVRIRKPNAP
                     IKGVLDTVEVEIFRRRDQMGEKS"
     gene            8765..9304
                     /gene="folK"
                     /locus_tag="PLO58_01730"
     CDS             8765..9304
                     /gene="folK"
                     /locus_tag="PLO58_01730"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQO73541.1"
                     /translation="MKSSGVFVALGSNLNLREKNLSAALELLSHNPAISICRMSAVYE
                     TAPKYNPGQPKFLNMVVEIATTLTPLQLLEFCKDIEVRLGRHFPHFKNSPREIDLDII
                     FFNRLIVESPTLTIPHPGLYERKFVLVPLAEIAPEFICPKTAKSVAELLRICPDIEQV
                     VRIGQLDELLTIPVTTGVL"
     gene            9301..9945
                     /locus_tag="PLO58_01735"
     CDS             9301..9945
                     /locus_tag="PLO58_01735"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQO73542.1"
                     /translation="MKNLYFLAIEGVIGAGKTSLARLMAEKLNARLVLEKFEENPFLE
                     DFYRDPERYAFQAQLYFLLSRYRQQIELKQIDLFTNLLISDYMFAKDKLFAILNLDEK
                     ELSLYNLVAQHLEKDLPKPDLVVYLQASTDRLISNIKKRGRSYEKSINRDYIDSLNQI
                     YNEFFFRYKDSPLIIVNTNEIDFVNNENDLNELIREIKQPISGTIYYNPAKKIL"
     gene            9993..10142
                     /locus_tag="PLO58_01740"
     CDS             9993..10142
                     /locus_tag="PLO58_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73543.1"
                     /translation="MVKPRNLRRPKPNNPKPKKPRTTLKIDKSQIEDAKFREIKEEPE
                     KEPKD"
     gene            10204..10785
                     /locus_tag="PLO58_01745"
     CDS             10204..10785
                     /locus_tag="PLO58_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73544.1"
                     /translation="MILVFFIFGQTPDDKPSGWRTLIAEHLNRYPAMQPADVYKLVYQ
                     GTFGPAHLGNDSIQISTSLENELAIITPDSSAPLSESITPSGKYVRLNLKRCKAEQIQ
                     TAKIVIAILNSIPQSSADSSRFIRRWETIEKLVVEGSLPIPRSDFFTFSDSIKKTGYP
                     VIHHSSQYIKNYQPAYRVIDRRYLNLISDSLSQ"
     gene            10871..11776
                     /locus_tag="PLO58_01750"
     CDS             10871..11776
                     /locus_tag="PLO58_01750"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQO73545.1"
                     /translation="MKKLVITGASGFLGGHIANLAVSRFQTLGLYSTYPFNLKGITTQ
                     RIELSSPSSIIVVLKRFKPAVVIHNAALANPDQCENQPDFARQNNVIATEHIASWCHK
                     NNARLIYVSTDMVFDGQKGNYSETDRPNPLSVYGKSKYEAEKLAMANNPDTVVARLAL
                     MYGCGIFPRAYNSEWLLRELLQRKKEPKLPPLKLYNDQYRSMLMVTNAAQALIELALS
                     HFCGIIHIGGPERISRFAFGEKLCRKLNLPLTFIQAVPYDEHQLTAPRPRDLSLDISL
                     ARSFLKTNFLAVDDGLDEMLMSNSD"
     gene            11788..12783
                     /gene="dusB"
                     /locus_tag="PLO58_01755"
     CDS             11788..12783
                     /gene="dusB"
                     /locus_tag="PLO58_01755"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQO73546.1"
                     /translation="MDSVQKITALTIGKVCIKPPLILAPMAGYTDQAFRQICKANGAG
                     LVCTEFVSSDGLVRSSEKTYDYLRFSDFERPISMQIFGNNPVVMAEAANLIENEFHPD
                     LIDINAGCSVRKVIKKNAGSALLKNLPLLEKIARAVVEAVSVPVTVKMRSGWDRQSII
                     AVETAQRLEQTGIKAITIHPRTAVDGFRHPADWSLIKEIKKKISLPIIGNGDIFTPAA
                     ALRMFAETNCNAVMIGRGALENPWIFRQIADLMENKPPISYDAPTERVKLCRRHLEME
                     IQYRGEVVAHKEMKKFYRWYLRGFGHVSEIREKLVRSKSVQEAFNILDELAHVTK"
     gene            complement(12841..13428)
                     /locus_tag="PLO58_01760"
     CDS             complement(12841..13428)
                     /locus_tag="PLO58_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73547.1"
                     /translation="MKKTLVVFCVVVILILASLATAGTPTTQKGSAAMWFNFNGLSDM
                     SYQNSAIGLNYLFKDKTGAGIALNFSHDSEDDGIEEKEKTTALGMDLYLIHYPFQKGP
                     VALYLAPGVGFNFSSNKDKDFGDIHINSTYMWLGGSVGVEWWIFDQVSLSASNWVGLE
                     IDLLNDKENDDKMTDVHLGILGSSTASIVLAFYFK"
     gene            complement(13467..14645)
                     /locus_tag="PLO58_01765"
     CDS             complement(13467..14645)
                     /locus_tag="PLO58_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HQO73548.1"
                     /translation="MNIEQIQTEIRQQNVDGWLFYDFHNRDKMAYHILGLNPNMFVTR
                     RWYYFIPAEGIPIKLVSAVEATKIDNLPGEKIIYRSWEQQHSSLRTMLKNSRRIAMQY
                     SPNNNIPYISVVDGGTIELIRSFGVEIVSSANLVQIFEAVIDEEGYQSHLRAGQKVQQ
                     IKNEAFALISSRIKEQKSVTEFEVQQFIVKRFGEEDLTDDNEHPIVGVNEHPADPHFE
                     PKAENAYTIKPGDTVLIDLWAREQSERAIYYDITWCGFVGQNPPAKYVEIFNIVVRAR
                     NAARDFIRRKMADNQPCYGYQVDDACRNVVKEAGYGDYFVHRTGHSIGHYVHGNGVNI
                     DNLETKDERELVPGICFSIEPGIYLPGEMAVRSEINVFITKDREVVVAGDEQEKLLLL
                     "
     gene            complement(14657..15199)
                     /locus_tag="PLO58_01770"
     CDS             complement(14657..15199)
                     /locus_tag="PLO58_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HQO73549.1"
                     /translation="MKLKNERSVLVIIDVQTKLLSVMYNYPVLLENLKKLIRGSQVLD
                     IPIIYTEQYPTGLGPTHPEIASLLSNTPPIIKMAFSCCSEDNFMNEIHKINRDQIIVC
                     GIESHICVYQTCRDLIDKKYEVHLVTDCIASRKPENTQLTVSKLLAYGVHPTSVEMAL
                     FEMLKDASNDAFKKIAKIIK"
     gene            15665..15859
                     /locus_tag="PLO58_01775"
     CDS             15665..15859
                     /locus_tag="PLO58_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73550.1"
                     /translation="MKNLVLISGVLGVLTFLMGVITQLVGHSILFCAASWNSLTQTML
                     LFAIAFAVWGHEKCCKKEKE"
     gene            complement(15948..16337)
                     /locus_tag="PLO58_01780"
     CDS             complement(15948..16337)
                     /locus_tag="PLO58_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQO73551.1"
                     /translation="MKEIILKTNRRIELIDITAQVAEAVRSSGIREGICVVYCPHTTA
                     GLTINEHADPNVASDIVNRLSKLVPHNENYAHIEGNADAHIKSTLTGNSVQLIVNNGN
                     LLLGVWQGIFFCEYDGPRNRRVWVQVK"
     gene            complement(16352..17623)
                     /locus_tag="PLO58_01785"
     CDS             complement(16352..17623)
                     /locus_tag="PLO58_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HQO73552.1"
                     /translation="MKRNRPLIVLIGLILILANLSFAQKILIPMDQTQSDHLKAYGIV
                     YWSLKQGVNVEWLLNYRGGSFLLDNYPGIVKELRLRGVAYEEVSAAQNLQIYATIEEN
                     NMDVVLLEKAAKIAVYSPPNKQPWDDAVTLVLTYAEIDYDVLWDEEVLTGKLPEYDWL
                     HLHHEDFTGQYGKFYASYHNQAWYQNEVAENEAIAAKLGFRKVSEEKKAVARTIKKYV
                     NEGGFLFAMCSATDALDIALAAENVDIVAEIYDGDPADPRANEKLDYSKCLAFENFKL
                     YLDPMLYEYSDIDYPPSFQAVAPGAEADYFTLFEFSAKWDPVPTMLTQNHVGVIKGFM
                     GQTTGFRRDRIKKSVVVMGEVVNGEQVRYIHGNHGKGTFTFLAGHDPEDYQHFVGDPP
                     TRLELHKNSPGYRLILNNILFPSARKKERKT"
     gene            complement(17629..19569)
                     /locus_tag="PLO58_01790"
     CDS             complement(17629..19569)
                     /locus_tag="PLO58_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73553.1"
                     /translation="MKLEWRLGLLILLIFWLTAFVAAQPVKVVSPEEKLMNLRFNQAQ
                     RLEQEGQLTQAAEIYRALVDAQPANYLYYSNYVNLLFRQKNLPELERVITRFMQLNPL
                     HENAAIDLGKLFYVRGDSAEAFREWQKAVVKFNHSVSFYRALFNEMVGLRLYDEAERL
                     IAAARAHYRQPDLLTMELANLQIARGKYSSAARELVLYARANPHYYNMIAGQILRFPT
                     DSTLFIQIDSLLKAELLLLPDQAALHRLRADFLFKNGHYNEALEEIFQVEALTGNHGD
                     QALAMAKNLVQIRRYELAQDFYTKILAKNELQGIAPQALLGLAESFEKVVLEQTTAEP
                     LHYFYPGNFFFNTDFVQQINQEQSSLQKAFAIYDSLVANQPNSIYSAQALYRLADLRF
                     RAVRDFDGALNLLRQAQNITRDRQLILNCALRIGEVQIARGDPDAALKYYQAEIVRWE
                     GTPGEKDLRVHLALAQFLAQEFDSLDIELKNLIPLLGPKHALFNDVVGFDGFFRANYS
                     ETDAAGKKAFSEFATAELLLRQNKLSEAEKIYAFILQNYPQTPIISAARFRLTQILLQ
                     FNRYAEAEATAESFFASENENADQIAFMLAEVADYREANYQTAAHYYELILEKYPNSF
                     LIDNVRKRLRELQRLSGVKKEI"
     gene            complement(19566..22958)
                     /locus_tag="PLO58_01795"
     CDS             complement(19566..22958)
                     /locus_tag="PLO58_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73554.1"
                     /translation="MGNNQSSYNRIERKLNDLRRQFARHVLQVGLLKVLLLIIIVTFL
                     IVVVEGFRYFKGSVRSDILFFTVGFSLIFLAIPIVYYFQIIRNRISAYDDYHLARRIG
                     ADQPEIKDDLLNALQLRELLNKETRGYSSELIEQALERIARKIEPLNFDRIIPSQEVR
                     KSLRNLAIAIFCLVICIASSPAYFKNSTERLLHPRIEYPVKLPFSIESTRASSGVLGG
                     DSVTISFQCQGRFPHYLTLDLQYPDYTQQEVLVVDDSGRSQYRIANLRHDLVYSAYYQ
                     NRSPFKPWRRIGTPPDTIRVINRPEILYVKSRFFYPEYTGLPAQTQETKSAEFNLLAG
                     TKISLEIRINKEIKEGHLSFASGNVLNLATNGDRARGEFIVKNDDQFEIIVADEKQVT
                     NINPIKYRLSVIPDAYPTITMLSPKNDLDLNADMVIPLGIRISDDFGFTRAAIRYRVI
                     KKYADYQEEETEITFPLAKLNITWQEVFYPWQIGELRLGPEDAVEFRVDIYDNDNISG
                     PKKASTDWLIARFPSLNELFSSVNEQQDEIYSSSEEVLKSLETTKKVLDEISRELLKK
                     PELKWEQKKQLEEEMKKTREAGEKITKLSEQLDDLIEKSRENQLFDAETLEKLAQVQE
                     AFQDIMTPEMREALDKLQAAIEKMDPKEVQKALNNFKTSQEQFSQELDRLLKIFRRIQ
                     IEQNIDELVRRFADLSERQKQVADELKKTAPDDQQKLNELAQAENAIKNDTEIAQDIM
                     KRTAADMAEFPMMPNKELQQLIDEMQAMDLPQEQSRASSAMRKGDKSQAEESAQNSQQ
                     NLQKLLERMEEFRTEFQRRNMADVMTDFRRILNKTMQLSYRQEELTEEIKNTPRQSDQ
                     IMDIAVKQSETRQNLSRLISDLIDLSGKTFGVSPRIGKNLGQSANSMNAAIAYMEERD
                     PGLAALSAENATASLNMTALELMAAMQELQKSGSASGFENYLQQLQELAGMQQGLNEE
                     TQMLGLGSPGDQGTLQQLAARQQQIRQSLEKLRDEMQGQGSTKQSGDLGGIAKDMDEV
                     IKDLENNQVLRRTLERQQRILSRLLDAQKSLRTQDFKEERQSKTGQEIVRESPDGLPS
                     NLGERRTLLQENLEKALRDGYSKEYENLIRRYFEELSKESVE"
     gene            complement(22959..23606)
                     /locus_tag="PLO58_01800"
     CDS             complement(22959..23606)
                     /locus_tag="PLO58_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HQO73555.1"
                     /translation="MVILLAVLNLAGQSKNLYGKFTIARLKYDGGGDWYSNPSSIPNL
                     LKFLRENLNMDVASDEVRVGIMDKDFFAYPYLYMNGHGNVRFSDEEVLRLRQHLQNGG
                     FLHADDNYGMDQSFRREIKRVFPDNDLVELPFDHPIYNILYKFPHGLPKIHEHDGKPP
                     QGLGIFYKGRLVVFYTYECDLGDGWEDPEVHNDPEEKHQAALQMGANIVLYSLMQ"
     assembly_gap    23615..23714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23715..25500
                     /locus_tag="PLO58_01805"
     CDS             <23715..25500
                     /locus_tag="PLO58_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73556.1"
                     /translation="RYCYFLSVARFVKCILIIGVLTVSLSAQWYFKIVDAIHGPIEYR
                     TPFKLTPFEFKTGIQTFGFDGQPLLFDSPGISFNGYKTSWERTMNSLELDLLKFNWLV
                     YILPQNLLDFQTGIGAKYTYSLSEQKLPETWPQRLPGSVDQLFLSPRIYEFNLNQTVI
                     YQWAPNFYNYLTISYGQASGSAYKTHSNDYFLHQKGHTYSFALGFKFLGSINYKLKEG
                     YGVEIRYTVGDFGDLEDPHNISPIKKINFNTVGISLTFNSNLGGGRSTGDEAQALYQA
                     NDFIAAKATFEQFIADNPHHPSRFKARRMIKECDKRIPYQEVTLAELFIARQNYSIAT
                     EYLARAQVTKDKTLLARINSNYERMIKQFVDTMNVMISNNRIDEAEQFLNTIAKLNLP
                     NTNELIYCYYSEIYFHRGAVFTEYGYWEKAIDYFDRAIQYYPPIRQRIEPYLVQIADG
                     YIKDANLSVDKKSIALALESLKQATALRPDIHYLTAPHIKNLEAGIEYLKQQAAKQKA
                     QESISKTFNPPPDAPKPDIGMSADQIKSILGQPGSQTRLETSGGRIYELWIYLFPDGK
                     ELQIYFDNAKVVKMEALSPIDVISEMDE"
     gene            25501..25995
                     /gene="rnhA"
                     /locus_tag="PLO58_01810"
     CDS             25501..25995
                     /gene="rnhA"
                     /locus_tag="PLO58_01810"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQO73557.1"
                     /translation="MKIIYSDGACSGNPGHGGWATLIFNENQLLKVLAGYQENTTNNR
                     MEIIAALNGLKQLQPGEKAIIYTDSQYLRNGITTWLPNWKKRNWKTADKKSVKNQELW
                     LELDRLNHPGITWQYVRGHSDNLYNEICDCLARKMIASQGKVSDLNAEFEKYYWQLQK
                     KKKQ"
     gene            complement(26003..28231)
                     /locus_tag="PLO58_01815"
     CDS             complement(26003..28231)
                     /locus_tag="PLO58_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQO73558.1"
                     /translation="MKSLEDLNPEQCRAVEYNAGPTLIFAGAGSGKTKVLAHKVAYLI
                     ENKIVAPQNILAVTFTNKAARELGERVEKYIGKKARQVNIGTFHSICARLLRREIECL
                     GYSSNFTIYDAEDQLSLLKKIINDRSIILESTSPKNVLSRISYLKNHMIEPENFSARP
                     GFMADRFVAVLYPLYQGALRKAMAVDFDDLLILPLKIFQLKPEVLEKYRALYQYILVD
                     EYQDTNRPQFLLIEALGKKHQHVCVVGDDDQSIYSWRGADIRNILSFNEIFPQCRIFK
                     LEQNYRSTNNILKAASAVVSHNKQRATKTLWSQKNDGDLIVKLDAETEYDEAALIASW
                     IQREILQNKRNFKDFAILYRTNAQTRVLEEILRRNHIVYVIVGGVRFYERKEIKDVLA
                     FFNVISNPHDDISLKRIINLPPRGISKTTVQHLEELALAKDISLFDAMSYADELNLAT
                     RAIESIKKFYDQINDLRSLRNTLSFEEWARVVIVSIGLRNYYKESEGEEATERLANLD
                     ELLNDISEFCQTNEKPSLESYLENVTLATSIDEWEDTKNAVTLMTLHGAKGLEFPVVF
                     ICGLNQGLLPLERDLTHEALEEERRLFYVGLTRAKEKVFLSSSRQRMRAGEVAYSSES
                     MFLNEIPPELITICDFREGIISSKRQPRTSNRSTARKIKDTFERVESNPIKIAGINSS
                     SSEKTSPTSRTIEPGTHVSHKIFGTGQVVNIDGVGQNARVTVNFRSCGRKTIVARFLN
                     IN"
     gene            complement(28228..>29128)
                     /gene="mltG"
                     /locus_tag="PLO58_01820"
     CDS             complement(28228..>29128)
                     /gene="mltG"
                     /locus_tag="PLO58_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQO73559.1"
                     /translation="VQEVSELLYEKGIIKYPKRFILATKMMGKERKLCAGRFDLYGAI
                     NYYELIKTLSDLESVTVRVTIPEGLRAREIARLLENQIGISADDFMQKVMDSRFTHQL
                     GVEANNLEGYLFPDSYHFIVGDSPENVISRMVERFWEVVNDSVQAEIRRTGYTLHQII
                     TLASIVEGECQVDFERPLVASLYYNRLRRRIRLEACPTIQYIIPDSPRRILRQDLKID
                     SPYNTYLHYGLPPGPVCNPGFKSIKAAIYPAKTKYLYMVSNGDSTYTHTFTANYEEFL
                     AAKRKLQKIRTQDKLVASEDYIN"
     assembly_gap    29129..29228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29229..30208)
                     /locus_tag="PLO58_01825"
     CDS             complement(<29229..30208)
                     /locus_tag="PLO58_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQO73560.1"
                     /translation="MLDITTKENIILYLSQWIEIVRIEDRDKYLVIEGRQRDTTSLRK
                     IARNSRNLISSIKKISTGSIDRFIVKPGKIEKRIPTVNLVLFLITILTTLLAGAMMEG
                     ANPFAHPLDLLKGVPFSVTLMVILGFHETGHFLMARKHNVDATLPYFIPAPTIIGTFG
                     AFIKMRSPVTRRQALLEIGAAGPICGFLVATPALFIGLKLSEVVSLSEAIGIQLGDSL
                     LMKIASALIFPNLEVGQDILLHPVGFAAWIGMLVTMLNLLPIGQLDGGHIAYALLGDK
                     YPYVALGALIATLALSFVSLNWLVWGLLVFFLVRVKHPPIYDDNSPVTLR"
     gene            complement(30520..33249)
                     /locus_tag="PLO58_01830"
     CDS             complement(30520..33249)
                     /locus_tag="PLO58_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HQO73561.1"
                     /translation="MISSLKRLLLLSGCFVTVTMAQELHTPAPQPISGTPFLWNKYPE
                     IVFHHRLAKPVAGDTTFFIRKNVEDNSAEFIEIAFLRLINTAEIEIYAEKTEFDAGRI
                     TQSDAERVRDIMLYDTPPGSINPAKGIYYNEIDIFGALPDKDGNGKLFVLLIDVRDNY
                     EAGKSDTYVAGYFDPLDQVPGKGNYGEIIYIDTNPADAKDLGTLSIVAHELQHLIHNF
                     YDSDEDVWVTEGFSELAPRLLGLPARSFASFLNETNRSLMDFDGSIADYAKVGLWTYY
                     IYQRFGLDVIKKIFNNKNDSFNGFIGGLSSAGYDGSVAELLRDWFVANLLNDPTMGDG
                     QYGYGGAAISPISSNYFHSNFTEGEQISLALNPAAAVYIQFYSGQNIEFVMNYQSSYS
                     FGLAVIKHYETPEITFVTCNGTGTYNFSDETFGSDYAKLTIIPYWPAEIAATVQANIS
                     YSATGIGGYAESELGYDNDMLDYFIRLEDAEAAEKFDLNLSEATLSAIKILTYDSSPV
                     TIKIYSNLAEAPLLTMSGIIPDEQAWTKIILGNPIDLADLSSFAVSVTSSGNSLGYSS
                     AQDGFGHAYIKTDEIFHDLCNYTVTGGSEDVSLTGNWAIRAIVSGKIVIPPRIILTPD
                     SLWFWHDEYTQTFRISNSGTEALEWEISGELPNWVKIEPLFGTVNIGSAEIAVTIDRN
                     LLTSGFHDINIPIISNGGDESLYVSVLKRNTSTAQSALIPTEMVFSDDISRTTLPIFN
                     IGIGSSQFAFWSEEACLAFLPSSGFIPENDTINVEVFLDRSAASDSILAFYFFDGVDT
                     LAYECIYSDWQGETATHFTILNPFPNPFLPGENPLIYFPFRLTVEKPVTALIFNINGQ
                     EVYSEKINNPKIGLNVWTWDGHNQKGNPVASGVYFIVFRQGERRARQKLVLLR"
     gene            complement(33246..34256)
                     /locus_tag="PLO58_01835"
     CDS             complement(33246..34256)
                     /locus_tag="PLO58_01835"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQO73562.1"
                     /translation="MSIAVLGAGSWGTTIAIHLYNKKLPVKLWEYFPENVAYMNKTRR
                     NPLLEGIPIPRAIPILNDLTEAVKNANFLVLAVPSQSMRSLLEKIKGQIEPDTIIVNL
                     TKGIEQNTLKRMSELITEILNHPPEKIVTLHGPSHAEEVARQVPTAVVAASINEETAQ
                     LVQEVFHSPYFRVYTNTDIIGVEIGGAVKNVIAIAAGICDGMRLGDNAKAALMTRGIM
                     EIVRLGLRVGGREVTFAGLSGIGDLIVTCNSQHSRNRYVGEEIGKGRKLDDILNGMSM
                     VAEGVTTCLTVHQMAEKYKVVMPISEEIYKVLFKNKDPQTALWDLMNRDPKQERHSLS
                     KY"
     gene            complement(34291..35283)
                     /gene="purM"
                     /locus_tag="PLO58_01840"
     CDS             complement(34291..35283)
                     /gene="purM"
                     /locus_tag="PLO58_01840"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQO73563.1"
                     /translation="MSVSYKDAGVDIAAGEQAVKEIKNLVKQTFTPNVLTEIGLFGGC
                     FAFPVKNYRQPVLVASTDGVGTKLIVANLLDRHNTVGQCLVNHCVNDILTTGARPLFF
                     LDYIGTGQLKPAITTEIIRGISLACKANGCALIGGEMAEMPDIYQTKDYDLVGTIIGV
                     VEREQMLIGRVGKGDVLLGLPSTGLHTNGYSLARKVLLTKYSVTDYIPELNATIGDAL
                     LAIHRSYLNVIEPLLADPDLHGLSHITGGGIIGNTSRIVPENLTLKINWQAWEWPPLF
                     KFIQKTGEIDIEEMRRVFNLGIGMIIICAASAVDKFSQVLQKSGEKAVILGEVC"
     gene            complement(35284..37095)
                     /locus_tag="PLO58_01845"
     CDS             complement(35284..37095)
                     /locus_tag="PLO58_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQO73564.1"
                     /translation="MSDFSYSTISEMFVGITDKYTDRPMYKYKKDGKWLGLTFSEVRN
                     RVEQISGGLKALGMKAGEHVAIIAANNPLWAMTDYGVICAKGVVVTIYPTLTAKQVWW
                     IARHSDSRFIFCGDREQTEKVIPLLNELPEVYKVIIMDDTKIDHPKAIMMSELMQMGA
                     EYRGQNPSAFLEDIHNTKEDDLLTIIYTSGTTGEPKGAMLTHGNLTSNITGALQIVEA
                     NENDTFLSFLPLSHGFERMAGHFLATGSGACISYAESIDTVAENMLEVHPTLMVAVPR
                     FYEKVYARVIDSISTSSKISQKIFWWAINVGKKAVFLKSENRNTGAILDNQLKIAQKL
                     VFSKLYEKIGGNLRFFVSGGAPLSKEIAQFFQAAGIPILEGYGLTETSPVISVNPLSK
                     CKVGTVGPKLPNIEVKIASDGEILTRGPHVMLGYYKDEASTRDVLDADGWFHTGDIGM
                     IDGDGYITITDRKKNILVTSGGKNVAPQPVENALVTSKWIEQIVVIGDRRKFVSALVV
                     PAFVNLEDWAKENNLQFHSREELIALPAVKELYDRVIAESMEGFAQFEKIKKYVLLPS
                     EFTIESGELTPSLKVKRSVVEKHYADLIESMYQEAES"
     gene            37164..37247
                     /locus_tag="PLO58_01850"
     tRNA            37164..37247
                     /locus_tag="PLO58_01850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37198..37200,aa:Leu,seq:caa)
     gene            complement(37341..38714)
                     /locus_tag="PLO58_01855"
     CDS             complement(37341..38714)
                     /locus_tag="PLO58_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73565.1"
                     /translation="MKKYLFIILIAFTTTLALPACSGGKETKKVERRTEQTKVTEKTE
                     PKKVQSPKTSTETTNLETVRKDTAGMVMQSVTAVKESQLDSVTPAKKDVQNEPDSTPL
                     TGNTSELAIQPSPVITDEIKIDADLLPTSNETNGVLESKVVTEKPGLCTKEAETSAKV
                     LKILIFDDIFFDADETETPNLTFNSNYIVTLNKVVKALKSDPDIKIRLKGYAMDTGTK
                     SGNAIIKSQKRAITVGKLILDLFPEEEKDSVANRIEIVPVGADEMLIESDNQVKELLN
                     HRVSIELFVGEVVNPTIADFVFHGKAPEPEVTSTAPKPVQREKAKTPKSRPRTRDMLY
                     ETGNDLFQQKKYEDAISVYTEFINLDPQHPLADNAQWWIGECYYFLGDYVSALNAYRK
                     VFGLGDGNKSAYAQLRMGYCYNRMNQVDLAVSAWEKVIRDYPNAIEEVTKAKKAIQIY
                     SGDVNQD"
     gene            complement(38794..39297)
                     /locus_tag="PLO58_01860"
     CDS             complement(38794..39297)
                     /locus_tag="PLO58_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73566.1"
                     /translation="MKELIKTIALVLVVVAVVYFFFEFEKVNKRLDILNARDSTHSVT
                     INDFDHNLHDLSLEFVGRGKHIQQFQQSLKDMNSRLDSVDLALSDKIEETNTSIAELK
                     IDIDSRFNVMKTDQDGINERLNKFQRETQRTLTDLQSAVTRMSRELSDLDKRVKTLET
                     PPTPAKK"
     gene            complement(39333..39650)
                     /locus_tag="PLO58_01865"
     CDS             complement(39333..39650)
                     /locus_tag="PLO58_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73567.1"
                     /translation="MEDKKVDFSIEESRKNFLITKFDDLFQGINNLYGKELMEELFHR
                     MEKTIAIFHQDVENLIKELKLTNSTGSTDRPIIGISDIPQTPAEPNPFAANEDNDWAK
                     HLG"
     gene            complement(39733..39918)
                     /locus_tag="PLO58_01870"
     CDS             complement(39733..39918)
                     /locus_tag="PLO58_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73568.1"
                     /translation="MLTRMTIELERSELLEFRQVLQQLTTNQPSVEIKLNSVIRGVCR
                     QIIREIDRWEDLKQERW"
BASE COUNT        11429 a         9024 c         7797 g        11497 t
ORIGIN      
        1 tagatgcctt tccagtagtt cttgatcgtg taattgggat caacgggttt aaaaacaatt
       61 ccacttccac tggctgtgaa gtcaccgtat actctaatta tcttattctg cgctatttta
      121 aaaacagttg atggttgtac aactaaatta ccagtctctg ctacagttac atcgccaacc
      181 atattaatat taccattcaa ggtcaggtca atcgggatac tgccctgaaa agctgagcga
      241 taccagtgat aataattggc tgtgaccgtg acgccgtcgc tgacaaacac cacgtccgtt
      301 tccggaccgc ttgaagacaa taccgctccg ccggacggat tgattgtcca gttataaaag
      361 actgcaatca aattaccatc gtcgacctct tgcggtacac ggagtgaata aattgggtac
      421 tgataattaa atccagagtt ttcattttca aaaacagtga aagactgtcc cgaggaatag
      481 ctgcggtagg cacaggggcg ggaataggaa gcttccactt tccagggatc cttgaattcg
      541 aactgaatat tacaattgcc tccaaaaaga taattggtac gaatttccga ctgaatcgct
      601 ttttcgaaat ttgatttttt caccagggac caatcaacaa cttgaatcga ttttttataa
      661 acgatatcat cttcactatc attccaacca tgttgttgag tgtaattact ggataccgta
      721 tatgtttcga attccgatcg gacatcgtga ctcgtattca gcggcagatt gataaaatgg
      781 ccggattcaa cattgtcatt ttcatctact accaatcgac cgaaattttc gatcccattg
      841 tattcgtttt tcagatatac ttttggatta aaacagtccg tacatggtcc cggtggatcg
      901 cccccaactt gatttaacca aattccaact gttgaaccat tggatatagg cactaatccc
      961 ccacttgccc tgtaaaactt gtcgtcgtca aaatcataat gaaagtacat atccatagga
     1021 taagaggtga atggtatcgg aggatctatt ccacattcag aatagtaaca atccagcaaa
     1081 ctaaacgtga accaggccga tttaccgggg tattcaacgg tgattgtgta ttcgccataa
     1141 gccaataagg gataaccact attatcgttg aaccaatcaa atgcagccca actccccccc
     1201 acggcagtag ttgtttgcgc agaatgatac agagtcgtcg gatcgtggtt gcaatcatag
     1261 acattcgaat tcgcgactgc gttgaacgaa attgttccat tccaaccgcc cggaagatcg
     1321 aacactttaa aggtgtattc tcccggtact cccaacaaaa tcgagaaact gccaaaaaga
     1381 aaagcaagaa atccaatgaa ctttttcatg ttacttcctt ttgttattct ctttttccca
     1441 tgagcataat cgattccggc ccgttggtgc cgaccgcgac gatcaaatct tccttaactg
     1501 aaatatcgta ataaacgata tcaggattcg attcatgata tgaataccac tcttttccgt
     1561 tgtaatgtat aatgatctgc aaatgccctg cggcaaagag atcgttgctg ccgtttccgc
     1621 ttacggattc caagaaattc ttgggaatat cctccggcca gggaatctcg gaaaccccgt
     1681 atccgtcgta atagaagagc ccggcgccaa ctatatatac atcatttggc gcagttgcaa
     1741 agactccctt tttcagtcgg gtgtcaaatt cgaaggtttt aatctggtca ttccgctgct
     1801 ctataaaaac atttttaccg gtggattcgt cgcttcccac catgaagata tgatctgccg
     1861 ctgttcccca gatatccgtg attctcagag aggtgccggt tggaattatc gtccagttct
     1921 caccattgta tcgaatgcaa aaaccattat taccgacaaa atagatgttt tccgaagagg
     1981 caccccaaca tcttttgatg ggataagcat ctatccccat atcgtgtaaa tgaaatagtt
     2041 gccaggaagt accattccaa tgtttgggat agtcagccat ccaaatatca tcgtatgaaa
     2101 atgcaaagac acaactagta gttttttcaa aaataattcg attgtcatag agtgtttcaa
     2161 ttctttgcgg tttccattcg acgccgtccc agtgcgtcgc gttataagta atgtagttgc
     2221 cggccgaatc gaggtcctgg ataaagccga cagcccagat gtcatcttcg gagataatga
     2281 atacatcccg cagataactg ctgagccagc cgattttttg aattttccag tcgacttcgt
     2341 ggctggttgt atctaaggtt gtaagcgtga ttacctccga agcattgatc tctttcctat
     2401 cataatatac cacaccttga tattggtagg cagttgccgg ttcagctgtt atatccgtga
     2461 ttagagtgtc ctttccggca atttgagatt tgctaatgac ttctccattc cgtttaatgc
     2521 caaactcgcg gacgtcgcca gaatcggaca cgctgactaa aagacggact ctggatgctc
     2581 ccacaaaatc agcggagata tcaatcgcat actcttccgg ttcggtcggg cattcccggc
     2641 aggacatggc gcagagcaga atacttatca gtaagatgat tgacatgacg gtcttgactt
     2701 tcaggttgtt atcagaagac atcgttttcc cttatttttt ctttcattgt cgaaagtacg
     2761 acccttttac ggtaatttca attcccctat actcatccat ttaaaactcc tatcagtttc
     2821 gctaccgcgg caatcatcgc ttccggcttg ggcggacagc ccggaatgta aaggtcaatc
     2881 ggtatgaccg cttctcgtga cggcacaata ttgtagctat ctttgaaaca acaaccgccg
     2941 gaaacgcaag tacctaccgc cactacgaaa atcggtttgg gtgcttgttc atagagcttt
     3001 ttaatcttgg gcgctacttt tttattgaac acacccgtca ccagaagcac atcggcgtgc
     3061 cgcactgaac cgaccaattt gatgccgaaa cgctcgacat catagcgcgg cgtcagtaaa
     3121 tccaggattt cgatgtcgca gttgttgcag ggtgagccgc tgacgtgaaa aacccacagc
     3181 gatttcgata aacgacgttt ataccatccc atatcaaact cctaaaaccg ctaaaatgac
     3241 cgccagcaaa gccactggcg tcagatattt ccagaaaaat tttagcgctg tatcaatccg
     3301 aacgcgcgga ttggtatttt taatcaaaac catcactacg acgattccga gatatttcaa
     3361 taccgaccat aaagcgcccc agccgatttc gctaaatcca ccgagaaata acaaaattaa
     3421 taaaaatgga aatgccaccc actgcatagc ctgattcaat ttccagaaac cgagcaatgg
     3481 cccggaatat tccatcagag gaccttcgac aatttcagtt tcggcttccg gaatatgaaa
     3541 cggcggcaga gtcattttag cttgaataca taaaagccca acaatgaatg ccagagctcc
     3601 ggaaaacgag ccgatgaaca ttccctccgc cacctgagcg ctgagtattt cctcaagttt
     3661 tatatggtag ccggatttaa ttattggcac caggcaaact aaaatgaaag ccagttcgtc
     3721 ggcaagaagt agtttgatct cgcgccccgc tccaagactg gagtaaacat tgccggtagc
     3781 cgatccgccc agaataacca taaccgaata aatcatcaat aagtacatta tcaccagcaa
     3841 gtcaccgcca aagccaattc cataaaaaag ggcgacgccc agcattgtcg tgatcaaagt
     3901 cgttacgcca aatgctacca gcggcgacaa cacaaaaaga gaatgcaggc tttcctttac
     3961 tattatagtt tctttgccga gtaatttgaa aaaatcgttg aaattctgga aaagcggcgg
     4021 gccaacgcga aattgcaccc gcgcgctgac tttgcgatca acccacccga ctaataatcc
     4081 cagaaccgca ctaaacaata atcctggaaa gaccaataga taaaaaaata cccatcccgt
     4141 catactttct cctccttatg tcgccagctc gggcttttga tcgccaaatg ttcaccatat
     4201 agaaaaatct gggcagatgg ctcctcctga ataatttctt ccatccgaat actatttagc
     4261 ggcgcggttt tggcgcaaag tggaacgtat tccggattat cctgcagttg attcaggcaa
     4321 aagtcgcact tgccggtcac gtaattaata acctctggaa aaatcgtccc gaaggggcaa
     4381 gccagcgtgc aggatttaca accgacgcaa cgcatattcc agcgtttgat caagccggat
     4441 tcggtgcgtt cgagcgcttc tttgggacag acggttacgc aaaaggcgtc ctggcattgg
     4501 cgacaataga ccgcaaattc ggcgatttcc agcagactaa actgcccgtt attacgacga
     4561 tgataaaaat attcacactc gattttttgc accgcatcac ccgcctcata taacattgga
     4621 atgtcaataa ataaacgttt cattcccaaa tattctcctc gtctttaatc tgttcataag
     4681 taaaaaccgg gccatctttg caggcaaaat acggacccat tacacaatga cgacattgac
     4741 cgataccaca gtacattttc ttttccatag ataaataaat attctgcggt ggatagccat
     4801 attttagcaa atcgaacgtg ccgaatttca tcatcaccgg cggaccacag acaatcaccg
     4861 ggttattatg cggatcaata gaaattttac tcagcaattt tgttactaaa cagacctctt
     4921 cgtcccagct ttgaccttcc ggaactttat caactgaacg cagaaaagta acttttggta
     4981 ttttgcgcca ttcgctaatt tgatctttat aaatcatatc agccggagtg cgagcaccag
     5041 ccaaaaaggt tacgctgtta taatcgtcaa tgtcatgtat taacgtcaga aataacgaac
     5101 gcgtcggtgc catcccgact cccccgccca tgatcaaaac gtccttatct ttgaattccg
     5161 ctaagggata acctttgcca taaggcccac gcaaacccag acgatcgccg actttggttc
     5221 gatgaatata ttccgttacg aatccggttt tcataatcgt aaaatccagg gtatcggcaa
     5281 tataatgcga agatgaaggc gtaaaaggtc cctcgccaat accgggtaca gttacctcca
     5341 taaattgacc ggttagaaaa ggcatcggtt tttccgggcg caaccgtaga gtacgaatag
     5401 tcggagactc ttcgataatt tcataaataa ccgccgcagt cgtttcatag ggattcttag
     5461 acattggctg cctccctgac attttcgaat gacataatcc gttttactac ctcacgaaat
     5521 tcgatttttc cagcgcaaac ttcaatgcaa cgtccacaac cagtgcaacc aatcaaccca
     5581 aagttgcttt gcatataact aaatttgcat aaatagcggt tgcgaaaacg ctcgaacatc
     5641 ttcggtcgag gcgtcgcgcc acctgccacc cgcgcgtaac cgtgtaactg acaggtatcg
     5701 gtactgcgat ctttggtaaa gatctcaccg ctggtttcgt cgtttagaat caggcagtaa
     5761 caggttggac aaatattggt acagccacca caaccacaac aggtaaaagc ttcgtcaacc
     5821 catttagtca aatcggtggc tttaagcttt tcatactgct gactacgttc atactgctta
     5881 ttttgtaata tcaaacgttt ttcgatggct ttgcggttct cgtcgcgcgt atttaaatca
     5941 tcttctaaat tccggctaat tttcacgttt ttctgcatta aatcgagtaa ttcctggccc
     6001 ttttctgaac cgacttccag atgataatac ttcgtatcga gtctggtgag attcaggtca
     6061 aaatttttag tagcataagg tcggccattg actaaattgc agtggcaggt cggcgcgatt
     6121 tcattacaat cggcactgat gattgtcaaa tttttacgcc ggtcctcata taacgggtca
     6181 acaaaatcag catttatcaa ggctttatcc aagacctcca gagcttgcag gtcacaggct
     6241 ttgacaccga cgatcaacca tttctcatca cttgtcatag ctggtaaaat tcgctcccgc
     6301 gccatataga aaagcattcg caagggatca atcgttcgat aactgtcaat cacaaattct
     6361 gccccttccc gccaattatc aaaaaataga acatcatcgg atttatcgga acgacgcggt
     6421 gcaatcactt gcttgaaatc acccagattt tctttctgac aacgcgttaa aaagtcgaat
     6481 aaagcggctt tttcaattat catcaataga cctccatctg aaaaactcaa tccataaagt
     6541 attttttaaa aactgattca ggctttaccc cctgccattt ttcggaccaa ttcgattaat
     6601 ttttctgatt cgctggattt atcgaaaaaa taatcagttc cttcttcgag acactttttg
     6661 cgatactggg gataaggata atcggtaaag acaattatac gcaaatcagg acgattttgg
     6721 cgcaccgttt taagcacata aatgccacca gcgcccggca tacgaatatc gaggataact
     6781 acatccggct tttgggtatc aattccgcta atagcagatt cggtatcctc accatagccg
     6841 acaatttcca cccctggtat ttctgttagc agattcgtta aatgcttacg aaggatttcg
     6901 gaatcatcgg cgataaaaac tttcatgctt aaaatatttt atctgaaggt gtgggattca
     6961 tataattcat gattaaatat aggttaatca ctccgacatg taaatcagac aaagcctgat
     7021 tttcttgtcg gtatttatcc tacaaggtct gatttaaatt gataaacaaa acctggcaag
     7081 tctataaaaa agaaaagccc ggctaagagc cggacttctc tcgattgagg gtgattggga
     7141 ggaaattatt tgaccttaac ttcaatctgc ttggcgagtg attctttctt tttaggaatg
     7201 acgatgttca atacaccatc tttaaattcg gcttcaatct tgtctgcctc gaccgaatca
     7261 tcgagtcgga aattccgttg gaatgaacca tagatgcgtt cagtgcgttg ataattctta
     7321 tcctcgactt tagattcgcg ttttttctcg ccgctaatag tcaggtaatt atctttcact
     7381 gcgattttga tatcatcttt gttcatacca ggcagttcaa cttggatgtt ataagcctta
     7441 tcctcttcga tgatatcaac ggcagggctg aatgctaata aactggtttc ctcttcatac
     7501 tgcggattga agattgattc gaacagacgg tcaaactcat tcctcacatc caaccacgag
     7561 cttcttggag tccatctagt aattgccata gcacacctcc tttcttttta gattagtttg
     7621 cttatttatt taatttcaat cgatttcggc ttactttccg gtgatttcgg aacaacgatg
     7681 gtcaaaatac catccttgaa ttcagcttcg attttctcat ggtcaactaa ctccggcaag
     7741 cggaaagaac gttcaaacga accgaatacg cgttcacatt gatagaaatt ctttttgttg
     7801 attttcttaa cccgtttctt ctcgccggaa atggtcagaa cattatcttt caggctaatt
     7861 ttgacatcat cttttttgac accgggcagt tcaacggtaa caacgtattc cgtatctttt
     7921 tcttcaatat caacttctgg agcaaaagtc actaaagatg tttctgtatc ccaatcgaag
     7981 aaaggatcga aaaaccgatc aatggtataa ggacttttcc tcgttacgct ttttggagtt
     8041 gctttagtaa ttgccatttc tacctccttt ttttattatt ttttactttc tcatttcaat
     8101 ttttcaaaaa ccgcttaata attactcaag tagtatgccg acccaaaaaa aacaccggat
     8161 attgtcagac tatttgcaaa aaacgacaaa gcactttgcc gatgtggcga tgtttggtag
     8221 aattggcatt ctttcctgac agattcacgc cagaagggca aaccatccag gggcaaattg
     8281 acctatcata tatttcctca atcaaaggat ttaatcataa atatgactta ctttcttgtc
     8341 cttttgacac tcattctcta aattccatta gtctaaagaa ttgaaaaatt atgggtattg
     8401 tcagattaaa caacatggtc ttttatggtt accacggagt agccgaacca gaaaaaatac
     8461 tcggcggcaa aatcgaggtt gatgtcgagc tggaatttga tatgactaag gtcattacca
     8521 cggatcatct ggcagacacg attgactacg aaaaggttta tcaattagtt cagggagtcg
     8581 taacccagtc caaatttttc ctgattgaag cattagccgg taaaatttta agcgaaatct
     8641 ttaatcatca tctggtagaa tcggtccgcg ttcgcattcg caaacccaat gcgcccatca
     8701 agggcgtgct ggatacggtc gaagttgaaa tatttcgacg acgcgaccaa atgggagaaa
     8761 aatcgtgaaa agttctggcg tctttgtggc tctgggaagt aatttgaact tgcgcgaaaa
     8821 aaatttatca gccgctctgg aattactgtc gcataatcct gcaatttcca tctgtcgtat
     8881 gtcagcggtc tatgaaactg ccccaaaata caatcccggc caaccgaagt tcctgaatat
     8941 ggtagtagaa attgccacca ccttgactcc gttacagtta ctcgaatttt gtaaagacat
     9001 tgaagttcga ttaggaaggc atttccccca ctttaagaat tcgcctcgtg aaattgacct
     9061 cgatataatc ttttttaatc ggcttatagt tgaaagcccc actctgacaa ttccccatcc
     9121 gggtctctac gaacgtaaat ttgtgcttgt gccactggca gaaatcgccc cagaattcat
     9181 ttgccccaaa accgctaaat cggtcgcaga gttgctaagg atttgtccgg atatcgagca
     9241 agttgttaga atcggccaac tggatgaatt actgactatt cccgttacta ctggagttct
     9301 atgaagaacc tttacttttt agccattgaa ggcgttatcg gtgccggtaa aactagcttg
     9361 gcgcgtctaa tggctgaaaa actcaatgcc cgtctggttc tcgaaaaatt cgaggaaaat
     9421 ccatttctgg aagattttta ccgcgatccg gaaagatacg cctttcaagc tcaactctat
     9481 tttctcttga gtcgctaccg ccagcaaatc gaattgaaac aaattgatct attcactaat
     9541 cttttaatca gcgattatat gtttgccaaa gataaactat tcgcaatcct caatctcgat
     9601 gaaaaagaat tgtcccttta caacctggtc gctcaacatt tggaaaaaga cttgcccaaa
     9661 ccggacctag tagtttactt acaagccagc actgaccgcc tgatatcaaa tatcaaaaaa
     9721 cgcggccgtt cttatgaaaa atctatcaat cgcgactata ttgattccct caaccagatt
     9781 tacaatgaat tcttcttccg ctacaaagac agtccgctaa ttattgttaa caccaatgaa
     9841 attgacttcg tcaataatga aaatgatctg aatgaactga tccgggaaat caagcaacca
     9901 attagcggga cgatttatta taatccagcg aagaagattt tataatgctc cgactattac
     9961 tactttttct gatcttttac ctgctttgga aaatggtcaa gccgcgcaat ctacgacgac
    10021 ctaaaccgaa taatcccaaa cctaaaaaac cacgcacaac ccttaaaatt gataaaagcc
    10081 aaatcgaaga tgccaagttt agagaaatca aagaagagcc agaaaaagaa ccaaaagatt
    10141 gataaactgg caaatttgac tatttccgtt tttttcttaa acatcctgat tatttcgtct
    10201 gtattgatat tagtcttttt tattttcggc caaactccag atgacaaacc ttccggttgg
    10261 cgcactttga tcgcagaaca tttgaatcgc tacccggcaa tgcaaccggc cgatgtatat
    10321 aaattagttt accaaggaac tttcggaccg gcgcacttag gtaacgactc aatccaaata
    10381 tcaaccagtc tggaaaacga gctggcgatt attacaccgg attcgagtgc cccgttatcg
    10441 gaaagcatca caccttccgg taaatatgtg cgcctaaatt taaaacgctg taaagccgag
    10501 caaatccaaa ctgccaaaat cgtcatagcc atcttgaact caatccccca atcttcagcc
    10561 gattcctcca gattcattcg ccgctgggaa actatcgaaa aattagttgt ggaaggctca
    10621 ttgcctattc cgcggtcaga ttttttcact ttttccgata gtatcaaaaa gaccggctac
    10681 ccggtgattc atcattcaag tcaatatata aaaaattacc aaccagcata tcgggtaatc
    10741 gaccgtcgct atcttaatct aataagtgat tcattatcgc aatgaaaatg gatttcattt
    10801 taccttcttt tggactaatt gacgccgatt atctgattgg cagtttgatt ctcgaaatct
    10861 ggcaaaagat atgaagaaat tagtcatcac cggcgccagt ggtttcctgg gtggacatat
    10921 tgcaaattta gcggtttctc gtttccaaac actcggactt tattcgactt atcctttcaa
    10981 tttaaaagga atcacaaccc agcgaatcga actttcctcg ccgagttcaa tcatcgttgt
    11041 cctcaaacga tttaaacctg ccgttgtaat tcataacgcc gccttggcaa accccgatca
    11101 atgtgaaaat caaccggact ttgccagaca aaataatgtt atcgctaccg aacacatcgc
    11161 cagttggtgt cataaaaata atgcccgttt gatttatgtt tcaaccgata tggtattcga
    11221 tggtcagaaa ggtaattatt cggaaaccga ccgacctaat ccgttaagtg tttatggcaa
    11281 atcaaaatac gaagccgaaa aactcgcgat ggcaaataat cccgatacgg tcgtagctcg
    11341 tttggctttg atgtatggtt gcggaatctt tccgcgtgct tacaattcag aatggctact
    11401 acgagagcta ctacagcgca aaaaggaacc gaaattacca ccgttgaaac tttataacga
    11461 tcaataccgc agtatgttga tggtcacgaa tgccgctcaa gcattaatcg agctggcttt
    11521 atcacatttt tgtggaataa tacatatcgg cggtccggaa cgcatcagcc ggttcgcttt
    11581 cggcgaaaaa ctctgccgca agctaaactt acccctgact tttatccaag ccgttccata
    11641 cgacgaacat caattaacag cgccgcgacc acgcgattta agtttagata tttcgctagc
    11701 ccgttcattt ttgaaaacaa attttttagc ggttgacgac ggtttggatg aaatgctaat
    11761 gagtaatagt gattaaaatg gaaaattatg gattctgtcc aaaaaattac tgctttgact
    11821 atcggcaagg tttgcatcaa gccgccgctg attttagcgc cgatggctgg ctataccgac
    11881 caagcttttc gccagatttg taaagcgaat ggagccggcc tggtttgtac cgaattcgtc
    11941 agtagtgacg gtctggtacg cagttcagaa aaaacttatg actatctacg tttttccgac
    12001 ttcgagcgac cgattagtat gcaaatattc ggcaacaatc ccgtggttat ggcggaagct
    12061 gccaatttga tagagaatga atttcatccc gaccttattg acatcaatgc cggatgctcg
    12121 gtccgcaagg tcattaagaa aaacgccggt tccgccttgc tgaaaaatct tcctttactg
    12181 gaaaaaattg cccgtgcggt tgtcgaagcg gtatcggttc cggttacggt aaaaatgcgg
    12241 agcggatggg atcgccaatc cattatcgct gttgaaactg cccagcgatt ggaacagacc
    12301 gggattaaag ctataacaat tcatccacgc actgctgttg atggttttcg ccatccagcc
    12361 gattggtcac tcatcaaaga aataaagaaa aaaatcagtt taccgattat cggtaacggc
    12421 gatatcttta caccagcggc tgctttaaga atgttcgctg aaaccaattg caatgcggtt
    12481 atgatcggac gtggggcgct cgaaaatccc tggatctttc gccagattgc agatttaatg
    12541 gaaaataaac cgccaatttc ctatgatgcg cccaccgaac gagtcaaact ctgtcgccga
    12601 catttagaga tggagatcca atatcgcgga gaagtcgtag ctcataaaga aatgaaaaaa
    12661 ttctaccgct ggtatttgcg cggcttcgga cacgtctccg aaatccggga aaaattggta
    12721 cgaagcaaat ccgtacagga agcatttaat attctcgatg aattggctca cgttacaaaa
    12781 taagaaaggt taatgaattc attaaccttt cctgaacctt aatttttaac gactgcagag
    12841 ttatttaaaa tagaaagcaa gcacgatgct cgccgtcgaa ctgccgagaa ttcccagatg
    12901 gacgtcagtc atcttatcat cattttcctt atcatttaat aagtcaattt ccagacctac
    12961 ccaattactg gccgataagc tcacctgatc gaaaatccac cattcgacac ccaccgagcc
    13021 acctaaccac atataagtcg aattgatatg aatgtcacca aaatctttat ctttattgct
    13081 agaaaaatta aatccgacac ccggagcaag atacaacgcc accggacctt tctgaaatgg
    13141 gtaatgaatt aagtataaat ccatacccag cgctgtagtt ttttctttct cttctattcc
    13201 atcatcctca ctatcatggg aaaaattcaa ggctatcccg gcaccagttt tgtccttaaa
    13261 caagtaattt agcccgatcg cggaattctg gtaactcata tcgctcaatc cgttaaaatt
    13321 aaaccacata gccgccgaac ctttctgggt cgtcggcgtg ccggcagtcg ccagactcgc
    13381 taaaatcaaa atcaccacca cacaaaaaac aacaagcgtc ttcttcattt ttaacctcca
    13441 ttgtttgtta aagacttcat tgtcatttat aataataaca atttttcttg ttcatcacca
    13501 gcgacgacca cttcccggtc tttggttatg aaaacattaa tctccgaccg caccgccatt
    13561 tcacccggca ggtaaattcc cggttcaatc gaaaagcaga taccgggcac cagttcgcgc
    13621 tcgtccttcg tttctaaatt gtcaatattg acgccattgc catgcacata atgcccaatc
    13681 gaatgtccgg tgcgatgaac aaaataatcg ccatatccgg cttccttgac tacattccga
    13741 caggcgtcgt caacttgata accgtaacaa ggctggttgt cagccatttt gcgacggata
    13801 aaatcgcgag ccgcattgcg agcgcgcacc acgatattaa aaatttcgac gtatttagca
    13861 ggcggatttt ggccgacaaa cccacaccag gtaatatcat aataaattgc ccgttcggat
    13921 tgttcgcgcg cccagaggtc tatcaatacc gtatcgcccg gttttattgt gtaagcattc
    13981 tccgccttcg gctcaaaatg cgggtcggca ggatgttcat tgactcccac aatcggatgc
    14041 tcgttgtcgt ccgtcaaatc ttcttcgccg aaacgtttga cgataaattg ctgaacttca
    14101 aattcggtaa cagatttttg ctcttttatt cgtgaactga ttagggcaaa ggcttcattt
    14161 tttatctgct gaactttctg accagctcgc aggtgcgact gatagccttc ttcatcaata
    14221 acagcctcaa aaatttgtac gagattagct gaagaaacaa tttccacccc aaaagagcga
    14281 attaattcaa tcgttccacc gtcaacaacc gaaatgtagg ggatgttatt attcggcgaa
    14341 tattgcatcg cgattcgccg actgtttttg agcatagtgc gaagactact atgttgctgt
    14401 tcccaactgc gatagataat tttttcaccg gggagattat ctattttagt cgcttcgacg
    14461 gccgaaacaa gttttatggg aatgccttct gccggaatga aataatacca gcgccgggta
    14521 acgaacatat tcggatttaa gcccagaata tggtaagcca ttttgtcacg gttatgaaaa
    14581 tcataaaaaa gccacccgtc aacgttctgt tggcggattt cggtttgaat ctgttcaatg
    14641 ttcattaaaa taacctctat ttaatgatct tagcaatttt cttaaaggcg tcattcgaag
    14701 catctttcag catctcaaag agtgccattt cgacactcgt tgggtgaacc ccgtaagcca
    14761 gcaatttgct taccgtcagt tgcgtatttt cgggcttgcg gctcgcgatg caatcggtta
    14821 ccaaatgcac ttcatacttt ttatcaatca aatcccggca ggtttggtaa acacaaatat
    14881 gggattcaat tccacaaacg atgatctgat cgcggttaat tttgtgaatt tcattcataa
    14941 aattgtcttc actgcaacaa ctgaatgcca tttttataat cggtggagta tttgataata
    15001 acgaggcaat ttcaggatga gttggcccta atccggttgg atattgttca gtatagataa
    15061 tcggtatatc cagaacctgc gaaccgcgga ttagtttttt aagattctct agtaagaccg
    15121 gataattgta cataaccgat aaaagcttcg tttgtacatc aataatcact aaaactgatc
    15181 gttcattttt tagcttcatt gctcgaccct ttctattgga acattgtttt tagatttagt
    15241 tattttagtc ctaaggttgc gaaaatgtaa ataaaaaagt attccaatta gaaggaagat
    15301 cagaattaag tttgttattg atttccgtct ccgactcatc gctaccatat ttctttaatt
    15361 aaatatggaa tatttagaaa aaataaacaa taaaaatatc gtcattttca cttaaacatt
    15421 ctgaatattt ttggctgatt tagtcatcat attcaacgat tcggaatcat ttgaactttc
    15481 tattgagggt gtcgcgctgg cgctatctgg agcttgtttt tttaattgat taaaatagaa
    15541 aacacccaaa aaccacgact atattctgat aattgcaact attttaataa taaacaattg
    15601 actttctgat aaaacactat taatattagc aaggtatttt acaatattaa atacggagga
    15661 ttttatgaag aatttagtat tgatttcagg cgttctggga gtattgactt tccttatggg
    15721 agttatcacc caattagttg gtcactctat cctgttctgt gccgcgagtt ggaacagcct
    15781 gactcaaacg atgttactat tcgccattgc ctttgctgtc tggggacacg agaagtgttg
    15841 taaaaaagaa aaagaatgaa tagtaattaa tcatcatctt ataaagtccc gggtcatccg
    15901 ggactttttt tatacagaat tatttattct ttagttattt aaaacgctta ttttacttgc
    15961 acccagactc ggcggttgcg cggtccgtcg tattcacaga aaaaaattcc ctgccaaacg
    16021 cccaacaaca aatttccatt attcacgatc agctggacac tattgccggt taaagttgac
    16081 ttgatatgag cgtcggcatt gccttcgata tgggcgtaat tttcattgtg gggcaccaat
    16141 ttacttaggc ggttgacaat atcgctggca acatttggat cggcatgttc attgatagtt
    16201 aaaccggcag ttgtgtgtgg acaatagacg acacatattc cttcccggat tccactggag
    16261 cggacagctt cagccacttg agctgtaatg tcaattagct cgatacgacg attggttttt
    16321 aaaataattt ctttcaattt ttattcctct cttaagtttt acgttccttc ttacgggctg
    16381 atggaaaaag tatattattc aatatcagcc gataacctgg tgaattttta tgtaattcca
    16441 accgggtagg cggatcaccg acaaaatgtt gataatcttc tggatcgtgt ccggcaagaa
    16501 aggtaaaagt tcccttgcca tgattaccat ggatatagcg cacttgctca ccgttaacaa
    16561 cttcacccat aacgacaacc gattttttaa tccgatcgcg gcgaaatccg gttgtctgtc
    16621 ccatgaatcc ttttattact ccgacatgat tttgagtcaa cattgttggc accggatccc
    16681 atttcgctga aaattcaaat aaggtaaaat aatcggcttc ggcacccggc gctacggctt
    16741 gaaagcttgg aggataatca atatcggaat attcgtagag catcggatcc agatacaatt
    16801 taaaattctc aaatgccagg cacttggaat aatccagttt ttcattggcg cgcggatcag
    16861 ccggatcacc gtcgtaaatt tcggcgacaa tatccacatt ctcagccgcc aatgcgatat
    16921 ccaaggcatc ggtagccgaa cacattgcga acaagaaacc accttcattg acatatttct
    16981 ttatagtacg cgctacggct ttcttttcct ctgaaacttt acggaagcct aacttagcgg
    17041 cgatagcttc attctcagcg acttcgtttt gataccaagc ttgattatga taactcgcat
    17101 aaaactttcc gtattgcccg gtgaaatctt cgtgatgcag atgaagccag tcatattccg
    17161 gcaatttccc agttagcact tcttcatccc agagaacatc gtaatcaatt tcggcatacg
    17221 tcagaaccag agttacggca tcgtcccaag gctgtttatt gggtggcgaa taaaccgcaa
    17281 tcttagcggc tttttctaat agcacaacgt ccatattatt ttcttcgatc gtcgcataaa
    17341 tttgcaggtt ttgagcggcg ctaacttctt cataggcaac tcctcgtaag cgtagttctt
    17401 taacaatgcc tggataatta tccagaagaa aagagccgcc tcgataattc aacaaccact
    17461 cgacatttac tccttgcttc aaactccagt aaacaatgcc gtaagctttt aggtgatcgc
    17521 tctgagtttg atccatcgga atcagaattt tttgggcaaa actcagattc gccaggatta
    17581 aaatcagtcc aattaataca attaaaggcc ggttgcgctt cattcgattc aaatttcttt
    17641 ttttacgcct gacaagcgtt gcagttcgcg tagacgcttg cgaacattat caattaaaaa
    17701 tgaattagga tatttttcaa gaattaattc ataataatga gccgccgttt ggtagtttgc
    17761 ctcacgatag tcggcaactt cagccagcat aaaagcgatt tgatcggcat tttcattttc
    17821 agaagcaaaa aaggattcgg cggtcgcctc ggcttcggca taacgattaa actgtaaaag
    17881 aatctgggtt aaacgaaacc gagcggctga aatgatcggg gtttgaggat agttttgtaa
    17941 aataaaggca taaatctttt cggcttcact caatttattt tggcgtaaaa gaagttcagc
    18001 cgtagcgaat tcactaaaag cttttttccc ggcggcgtcg gtctcgctat aatttgcccg
    18061 gaaaaaacca tcaaagccga ctacgtcatt aaaaagcgca tgctttggtc ccagcaatgg
    18121 tattaaattt ttcagttcga tgtccaatga atcaaattcc tgcgccaaaa attgagctag
    18181 tgccagatga actcgtaaat ctttttcacc tggtgttccc tcccatcgta caatttctgc
    18241 ttgataatat ttaagagccg cgtcgggatc gccgcgcgca atctgcacct cgcctattcg
    18301 taaagcacag tttaaaatta attgtcggtc gcgggtgata ttctgagcct gacgcagtag
    18361 attcagcgct ccatcaaaat cacgcactgc ccgaaaacgc aaatctgcca gccgatacaa
    18421 agcctgagct gaatagatgc tattgggttg attggcaact aaagaatcat aaatcgcaaa
    18481 ggctttctgc aaactcgatt gttcctgatt aatttgctgg acaaagtcgg tattgaaaaa
    18541 gaagttaccg ggataaaaat aatgcaacgg ctcagccgtt gtctgttcca gcacaacttt
    18601 ctcgaatgac tcagctaatc cgagtaaagc ttgcggagcg ataccctgca attcgttctt
    18661 tgccaaaatt ttcgtataaa aatcctgggc gagttcgtaa cgccgaatct ggactaaatt
    18721 tttagccatt gccagcgctt gatctccatg attaccagtc agcgcttcaa cctggaaaat
    18781 ttcctccagt gcctcattat aatgcccgtt tttaaaaaga aaatccgccc gcaggcgatg
    18841 cagagcggct tgatcgggca aaagtaacaa ctccgccttt agcaaggaat ctatctgaat
    18901 aaacaaggtc gagtcagtcg ggaaacgcaa gatttgtccg gcaatcatat tgtaataatg
    18961 cggattagcg cgagcataaa gtacaagttc tcttgccgcc gagctatatt ttccccgtgc
    19021 aatttgcaaa ttggctaatt ccatcgtcaa taaatcgggt tgtctataat gagcgcgggc
    19081 ggcggcaatc aagcgctcgg cttcatcata taatctcaag ccgaccattt cattgaacag
    19141 agcacgataa aacgaaacgc tatggttaaa tttcacgacg gctttctgcc attcccgaaa
    19201 cgcctcagcg ctgtcaccgc gcacatagaa aagtttaccg agatcaatag cagcattttc
    19261 atgaagcgga ttcaattgca taaaacgggt aatgacacgt tcgagctccg gtaaattttt
    19321 ctgccggaaa agcaagttga cataattgct ataatacaga taattagccg gttgggcatc
    19381 aaccaacgcc cggtaaattt cagcggcttg cgtcaactga ccttcctgct cgaggcgctg
    19441 agcctgatta aagcgtaaat tcatcagttt ttcttcgggt gaaacgactt ttaccggttg
    19501 agcggcaacg aaagctgtaa gccaaaaaat taataagatt aataatccca aacgccattc
    19561 gagcttcatt ctaccgactc cttactcaat tcttcaaaat accggcgaat cagattttcg
    19621 tattctttgg aatacccgtc gcgcagagct ttttctagat tttcctgtaa aagagtgcgc
    19681 cgctcgccaa ggttagacgg cagaccatca ggagattcgc gaacgatttc ctgcccggtt
    19741 ttactctggc gttcctcttt aaaatcctga gtgcgcagag atttctgagc atccaggagt
    19801 cgggagagaa ttctctgctg gcgttccagc gtgcggcgta aaacctggtt attctcgagg
    19861 tctttgatga cttcgtccat atctttggca atgccaccga gatcgcccga ctgtttagtg
    19921 ctaccttgcc cttgcatctc atcgcgtaat ttttcgagtg attggcgaat ttgttgctgg
    19981 cgagcggcta attgctggag agttccctga tcaccgggag aacccaatcc cagcatctgg
    20041 gtttcttcat tcaaaccctg ttgcatacca gccaattcct gcaattgctg aaggtaattt
    20101 tcgaaaccgg aagccgaacc gctcttctgt aattcttgca tagccgccat cagttccaga
    20161 gccgtcatat tgagcgaagc ggtggcattt tcagcggaaa gtgccgccag tcccggatct
    20221 cgttcttcca tataggcgat agccgcattc atactattag ctgattgacc gaggtttttg
    20281 ccgatacgcg gggaaacgcc aaaagtttta cccgacaaat caatcaaatc ggaaatcagg
    20341 cgggacaaat tctggcgagt ttcactttgc ttaacggcta tatccataat ctgatcgctt
    20401 tgacgcggag tgtttttaat ctcctcggtg agttcttctt gacgatagga taattgcatt
    20461 gttttattca aaatccgacg gaagtccgtc ataacgtccg ccatattccg tctctgaaat
    20521 tcagtgcgaa attcttccat tctttctaac aatttctgca agttttgctg agaattctgg
    20581 gctgactcct cagcttgcga tttatcacct ttgcgcatgg cactgctagc tcgagactgt
    20641 tcttgcggta gatccatcgc ttgcatttca tctatcaatt gttgaagctc tttattcggc
    20701 atcattggaa attctgccat atcagccgca gttcttttca taatatcctg agcaatttca
    20761 gtatcatttt taatggcatt ttctgcctgc gctaattcat tcaatttttg ctgatcgtct
    20821 ggcgctgttt tcttcagttc gtcagccact tgcttctgcc tttcgcttag atcggcaaag
    20881 cgcctaacca attcatcaat attctgttca atttgaatcc ggcgaaaaat tttcaggagg
    20941 cggtcgagtt cctgcgaaaa ttgctcctgg gaagttttga aattatttag agctttttga
    21001 acttcctttg gatccatttt ttcgatagcg gcttgcaatt tatcgagtgc ttcgcgcatt
    21061 tccggtgtca ttatatcctg aaatgcttcc tgcacctggg ctaatttttc caatgtttcc
    21121 gcatcgaaga gttgattctc acgacttttc tcaatcaagt catcaagttg ctcgctaagt
    21181 ttagtgattt tttcaccggc ttcgcgagtt tttttcatct cctcttctaa ttgctttttc
    21241 tgttcccatt tcagttccgg ttttttgagt aattcacggg aaatctcgtc gagaaccttt
    21301 ttggttgttt ccagagattt taaaacttcc tcgctactgg aataaatttc atcctgttgt
    21361 tcgttgaccg agctaaaaag ttcattgagt gatggaaagc gggcgattag ccagtcggtg
    21421 ctggcttttt tggggccgct aatattatca ttgtcataaa tatcaacccg gaattcgacc
    21481 gcatcttccg gacctaatct aagttcgccg atttgccagg gataaaatac ttcctgccat
    21541 gtgatattca attttgcgag tgggaatgtt atttccgttt cctcttcctg ataatcggca
    21601 tattttttta tcacacggta gcgaatagcg gcgcgggtga atccgaaatc atcgctaatc
    21661 cgaataccga gtggaattac catatcggcg tttaaatcaa gatcattttt cgggctgagc
    21721 atagtgatag tcggataggc atccggaatg acgctcagtc ggtacttgat aggattaata
    21781 tttgtcactt gcttttcatc ggctacaata atttcaaact ggtcgtcatt ttttacgatg
    21841 aattctccac gagcgcgatc accattcgtc gccaaattta agacattccc gctggcaaag
    21901 gataaatggc cttctttaat ttctttattt atacgaattt ctaaagatat tttcgtgcca
    21961 gccagcaagt taaattccgc gcttttggtt tcctgagtct gagccggaag tccggtatat
    22021 tccggataaa aaaaacgcga tttaacgtat aaaatttcag gtcggttgat gactcgaata
    22081 gtatccggag gagttccgat tcggcgccag ggtttgaacg gcgaacgatt ttgataataa
    22141 gcgctataaa ccagatcatg ccgcaaattt gcgatccgat attggcttcg tccggaatca
    22201 tctaccacta aaacctcttg ctgagtataa tccggatact gaaggtcaag cgttaaatag
    22261 tgcggaaatc gtccctgaca ctgaaagctg attgttaccg aatcgccacc tagcaccccc
    22321 gatgacgctc gggttgattc gatagaaaag ggcaatttga ccgggtattc gattctggga
    22381 tgcaaaagcc gttccgtgct atttttgaaa tatgcgggac tggaggcgat acaaattacg
    22441 aggcaaaaaa tcgcaattgc cagattacgc aaactcttcc gaacttcctg agatggtatt
    22501 atccgatcaa aattaagcgg ctctattttt cgggctatcc gctccagagc ttgttcaatc
    22561 agttcactcg aataaccgcg cgtttcttta tttagcaatt cccttaactg caaggcattc
    22621 agtaaatcgt ccttgatttc cggctggtcg gcgcctatcc ggcgtgccag gtgataatca
    22681 tcgtaagcac taattcgatt ccggattatt tggaaataat acacaattgg aatcgccaga
    22741 aaaatgaggc tgaatccaac cgtaaaaaat aaaatgtcac tgcgaaccga acccttgaaa
    22801 tagcgaaaac cttcgacgac aacaattaaa aaagtcacga ttataatcaa caataaaact
    22861 ttcagcagac cgacctgtaa aacatgccgc gcaaattggc gtcgcaagtc atttaatttg
    22921 cgttcgatcc ggttatagct ggattgatta tttcccattt attgcatcaa cgaatataaa
    22981 actatattgg cgcccatttg aagtgccgcc tggtgttttt cctcaggatc attatggact
    23041 tccgggtctt cccagccgtc gccgagatca cactcataag tataaaacac aaccagccga
    23101 cctttgtaaa atattcccag tccctgcggt ggcttgccat cgtgttcgtg gattttgggc
    23161 aaaccgtggg gaaatttata caaaatatta taaatcgggt gatcaaacgg caattccacc
    23221 aagtcattat cgggaaaaac tcgtttaatt tcacgccgaa aggattgatc cataccataa
    23281 ttatcgtcgg catgtaaaaa gccaccgttt tgcaaatgct ggcgaagacg cagaacctcc
    23341 tcatcactga aacgcacgtt gccatgacca ttcatataaa gataaggata ggcgaaaaag
    23401 tctttgtcca ttatgccaac ccgcacttcg tcggaggcga cgtccatatt gagattttca
    23461 cggagaaatt tcaacagatt cggaattgaa ctcggattgc tgtaccagtc gccaccgccg
    23521 tcatatttca ggcgagcaat cgtaaatttc ccatagagat tcttactctg cccggcgaga
    23581 tttaagaccg ccaataaaat aacaataaat gtccnnnnnn nnnnnnnnnn nnnnnnnnnn
    23641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnngaggta ttgttatttt ctatccgtgg ctcgattcgt aaaatgtatc
    23761 ttaataatcg gggtattaac cgttagctta tccgcacaat ggtatttcaa gattgtcgat
    23821 gctattcacg gaccgatcga atatcgcaca ccatttaaat tgacaccatt cgaatttaaa
    23881 accggcatac agacatttgg atttgacggt cagccacttc tatttgattc ccccggaatt
    23941 tcctttaacg gctataaaac ttcttgggaa cgaacgatga acagcctcga actggatttg
    24001 ttgaagttca actggctggt gtatattttg ccgcaaaatt tgctcgattt ccagaccgga
    24061 ataggcgcta aatacacata ctcattatcc gaacaaaaac tcccggaaac ctggccgcaa
    24121 cgtctgccgg gctccgttga ccaacttttt ttgtcacccc gaatctatga attcaacctc
    24181 aatcaaacgg tgatatatca atgggcgccg aacttttata attacttaac gatcagttac
    24241 ggtcaagctt ccggctcagc ttataaaact cattctaacg actattttct acatcaaaaa
    24301 gggcacacct actccttcgc gctgggattt aaattcctcg gcagtatcaa ttataaatta
    24361 aaagaaggtt acggcgtaga aatcagatac acagtcggtg atttcggtga tttggaagac
    24421 cctcataaca taagcccgat taagaagatt aattttaata ctgtggggat ttctctgaca
    24481 ttcaattcta atctcggcgg aggtcggagt actggtgacg aagcccaagc tctttatcag
    24541 gctaatgatt ttatagccgc caaagccacc tttgaacaat tcatcgccga taatccccac
    24601 cacccaagcc gctttaaagc ccgtcgaatg attaaagaat gcgacaagcg aattccctat
    24661 caggaagtaa ctctggcgga attgtttatt gcccgtcaaa actattccat agctaccgaa
    24721 tatttagcgc gagcccaagt gactaaagac aaaaccctat tagctcggat aaatagtaat
    24781 tacgaaagaa tgattaaaca gtttgttgat acaatgaacg tgatgattag caacaatcga
    24841 atcgatgaag ccgaacaatt tttaaacaca atcgcaaagc tgaatctccc aaacactaat
    24901 gagcttatct actgctacta cagtgaaatt tatttccatc gcggagcagt ttttactgaa
    24961 tatggttatt gggaaaaagc tattgactat ttcgatcgtg ccattcaata ctatccacct
    25021 attcggcaac gcatcgagcc atatttggtg caaatcgctg acggttacat caaggacgcc
    25081 aacctttctg tggataagaa aagtatagcg ttagcgctgg agtcgctcaa gcaagcgacc
    25141 gctctgcgac cggatattca ttacttaact gctcctcata ttaaaaactt agaagccggt
    25201 atcgaatacc ttaaacagca agccgccaaa caaaaagcac aggaatccat aagtaaaaca
    25261 ttcaacccgc cgccggatgc tcctaaaccc gacatcggta tgagtgccga ccagataaaa
    25321 agtattctcg ggcaaccggg ttcccaaacc cgcttagaaa ccagcggtgg aagaatttac
    25381 gaactgtgga tttacttatt tccagatggc aaagaattac agatttattt tgataatgca
    25441 aaggtagtta agatggaagc tttatctccg attgacgtaa tttcagaaat ggacgaatag
    25501 atgaaaatca tttactccga cggagcctgt tcaggaaatc ccggccacgg cggttgggcg
    25561 actctgattt ttaatgaaaa tcaattgctc aaagtcctgg ctggttatca agaaaatacg
    25621 actaataatc ggatggaaat catcgcggcg ctcaatggct tgaaacaatt gcaacccggt
    25681 gaaaaagcca ttatttatac tgacagtcaa tacctacgta atggaattac aacctggctt
    25741 cccaactgga aaaagagaaa ttggaaaacc gctgacaaga aatcagtcaa aaatcaggag
    25801 ctgtggctcg aacttgacag gttgaatcat cctggaatca cctggcaata cgttcgagga
    25861 catagcgata atctttataa tgaaatttgc gattgtttgg cacgtaaaat gatcgcaagt
    25921 caagggaaag taagcgatct aaacgccgaa ttcgaaaaat attactggca attgcagaag
    25981 aagaaaaagc agtaattatt tatcaattga tgttgagaaa acgcgctacg atcgttttgc
    26041 gtccgcagga acgaaaattt acggttactc gggcattttg tccgacaccg tctatattaa
    26101 ctacctgacc ggtgccaaaa attttatgag aaacatgcgt accgggttca atcgtccggc
    26161 tggtcggaga agttttttcg cttgaagaag aattgattcc cgctattttg atcgggttag
    26221 attcaacccg ctcaaaggta tcttttattt tccgagcagt ggaacgattg ctggtccgcg
    26281 gttggcgctt actggaaata ataccttccc gaaaatcgca gattgtaatc aactctggcg
    26341 ggatttcgtt gagaaacatt gattccgatg agtaggcgac ttcaccggcg cgcatacgct
    26401 ggcgggacga tgataaaaaa accttttcct tggcgcgagt cagaccgaca taaaaaagac
    26461 gacgctcctc ttcgagcgct tcatgggtca agtcgcgttc gagcggcaat aagccttgat
    26521 ttaatccgca aatgaagacc acaggaaatt ccaagccctt ggcaccgtgt aaggtcatca
    26581 gcgtcaccgc atttttagtg tcttcccatt cgtcaatcga agtcgccaga gtcacatttt
    26641 cgaggtaaga ttctagcgaa ggcttttcgt tagtctggca aaattctgaa atatcgttga
    26701 gtagctcgtc aaggttggct aatctttcag ttgcttcttc gccctcgctt tctttgtaat
    26761 agttacgcag gccaatgctg acgataacaa cccgtgccca ttcttcaaaa gaaagtgtat
    26821 tgcgtaatga acggagatca ttgatctgat cgtaaaactt tttgatagat tcgatagccc
    26881 gcgtagcaag attgagttca tcggcgtacg acatagcatc gaatagcgaa atgtctttag
    26941 ccaaagccag ttcctcaaga tgttgaacgg tcgttttact tatcccgcgt ggcggtagat
    27001 tgataatccg ttttaaactg atatcatcat ggggattgga tatgacgttg aaaaacgcta
    27061 aaacatcttt gatttcttta cgctcataaa aacggacacc acctacaatt acatacacga
    27121 tgtgattacg tcgtaaaatt tcctcaagaa cacgggtttg agcatttgtc cggtaaagga
    27181 tggcaaagtc tttgaaatta cgtttgttct gtaaaatttc tcgttgaatc cagctggcga
    27241 tcagggcggc ttcgtcgtat tcggtttcgg catccagctt aactattaaa tcgccatcgt
    27301 ttttctgtga ccagagtgtt ttggtagcgc gctgtttatt gtggctgaca accgctgaag
    27361 cggctttcag gatattattg gtcgagcgat aattttgctc gagcttaaaa atccggcatt
    27421 ggggaaatat ttcgttgaaa ctgagtatgt tgcgaatatc agcgccgcgc cagctataaa
    27481 tactctggtc atcgtcgcca actacacaga cgtgctgatg ttttttaccc agcgcttcga
    27541 ttaagagaaa ctgaggacga ttggtatctt gatattcatc aactaaaata tattgataaa
    27601 gtgcccggta tttttccaaa acttcgggtt taagctggaa tattttcagc ggtagaatta
    27661 gcagatcgtc gaaatccacc gccatagctt tacgtaaagc tccctgataa aggggatata
    27721 gcacagctac aaaacgatca gccataaatc cgggccgagc gctgaaattt tccggctcaa
    27781 tcatatgatt tttcagatag gaaattcgac tcaagacatt tttaggactg gtggactcga
    27841 ggataataga acgatcattg ataattttct tgagaagact gagctggtcc tcagcatcgt
    27901 agattgtaaa attcgaagaa taaccgagac attctatttc acgtcggaga agacgagcac
    27961 aaatcgaatg aaaagtgccg atattgactt gcctggcttt cttgccgata tatttttcta
    28021 cccgttcacc taattcgcgt gcggctttgt tggtaaaggt aaccgctaga atattttggg
    28081 gggcgactat tttattttct atcaggtagg caactttatg cgccagaacc ttggtttttc
    28141 cactcccggc accagcaaaa attaaggttg gaccggcatt atattcgacc gcacggcatt
    28201 gttcgggatt caaatcctca agtgatttca attgatataa tcctcactgg caacaagttt
    28261 atcctgggta cggatttttt gcaattttct ttttgctgct aaaaattcct cataattcgc
    28321 ggtaaaagtg tgggtatagg tactatcacc gtttgaaacc atatatagat atttggtttt
    28381 ggcgggataa atagccgctt ttatggattt gaaaccggga ttacagaccg gccctggcgg
    28441 taatccgtag tgtaagtaag tattataagg tgaatcaatt tttaaatctt gtcgcagtat
    28501 tcgtcgcgga ctatccggaa ttatgtattg aattgtcgga caggcttcga gacgaatcct
    28561 tctacgcaag cgattgtaat agagtgaggc aactagcggt cgttcgaaat ctacctgaca
    28621 ttctccttcc actatcgagg ctaaggtaat aatctggtga agagtataac cagtacgacg
    28681 gatttcagcc tgcactgaat cattgacaac ttcccagaag cgttctacca tccgactaat
    28741 gacattttcc ggcgaatcac ctacgataaa atgataggaa tcaggaaaga gatacccctc
    28801 cagattatta gcttcaaccc ccaattgatg agtaaatctg gaatccataa ctttttgcat
    28861 gaaatcatcg gccgaaattc caatctgatt ttcaagcaac ctagcaattt cacgggcacg
    28921 caaaccctca ggaatagtga cgcgcaccgt aaccgattcc agatcactca gagtttttat
    28981 caactcataa taattgatag ctccatatag atcaaaacgt ccggcgcata atttccgttc
    29041 ctttcccatc atttttgtcg ccagaataaa gcgtttagga tacttgataa tacctttttc
    29101 atataaaagc tcagatactt cctgaacann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29221 nnnnnnnntc acgtaatgtt accggagaat tatcatcata aatcggcgga tgttttactc
    29281 taaccaagaa aaacactagt aagccccaca ctaaccaatt aagtgacaca aatgagagcg
    29341 ctaaagtcgc aattaacgct cccaaagcaa cataaggata tttatcgccc agaagggcat
    29401 acgcaatatg tccgccatcc agctgcccga taggcaagag attcaacatg gttaccagca
    29461 ttccgatcca agccgcaaaa ccaaccggat ggagcaaaat gtcctgcccg acttccaggt
    29521 ttggaaaaat caaagccgag gcaattttca tcaatagtga atcaccgagt tgaataccga
    29581 ttgcctcact taacgatacg acttcagaaa gctttaagcc aatgaataaa gctggcgtcg
    29641 ctaccaaaaa accgcagatc ggcccagccg ctccaatttc caataaagcc tgccgccgcg
    29701 ttaccggaga gcgcatttta atgaaagcgc cgaaagtccc gataatagtt ggcgctggaa
    29761 taaagtaagg taaggtcgca tcaacattat gtttacgcgc catgagaaaa tgcccggttt
    29821 cgtgaaaacc gagaattacc atcagagtca cactaaaagg aacacctttg agcaaatcca
    29881 gtggatgagc gaatggatta gccccctcca tcatcgctcc cgctaaaaga gtcgttaata
    29941 tcgttatcaa aaacaagaca agattaaccg tcggaatccg tttttcaatt ttccccggct
    30001 tgacgataaa tcggtcaatg gaaccagtcg atattttttt tatcgaacta atcaaatttc
    30061 gagaattgcg ggcgatttta cgcaaagaag tggtatcccg ctggcgccct tcgatcacca
    30121 gatacttatc tcgatcttct attcggacaa tttcgatcca ctgactcagg tataaaataa
    30181 tgttttcttt ggtagttata tccagcataa catttcgttt aaattaccca atttttagag
    30241 aacttacaaa atacagcctt aaaatcaatc aaataattct tcgacttttg tctggcaact
    30301 tatcagtttc tgcttctaaa tgattgaact taatgattaa agatattcac gtattatgaa
    30361 aaattcgacc gaactttaga caccggcaga ctaaaaccgg ttacaataac ctacttgggt
    30421 aaagttcaca tttgaattca ggtatcaaag aaaatgaacc agaaaccgca ctgaatttca
    30481 ttttagttta ctctacatga aatgcatagt agccacatat tatctcaata acaccaattt
    30541 ttgacgtgcc cgtcgttcac cctgacggaa aacaataaag taaactccgc tggcaaccgg
    30601 attgcctttt tgattgtgac catcccaagt ccagacattc aagccgattt tcggattatt
    30661 gattttttcg gagtaaactt cctgtccgtt aatattgaaa atcaatgccg taaccggttt
    30721 ttccacagtc aatcgaaaag ggaaataaat caatggattt tcaccgggca gaaatggatt
    30781 cgggaaagga ttcaggatag taaagtgggt tgctgtctct ccttgccagt cagaataaat
    30841 acattcgtat gctaaagtat ctactccgtc gaagaagtaa aaggctaaaa tgctatcgct
    30901 cgcggctgat cgatccaaaa atacttccac attgattgta tcattttccg gaatgaatcc
    30961 actcgatggc aaaaaagcca gacaggcttc ttccgaccag aaagcaaatt ggctggaacc
    31021 tatgccaata ttgaaaattg gtaatgttgt ccggctaata tcatcgctga ataccatttc
    31081 tgtgggaatg agagcagatt gcgctgttga ggtatttcgc tttaaaacac taacatagag
    31141 cgattcatcg ccgccgttcg aaataatcgg aatattaatg tcgtgaaagc cgctggtcaa
    31201 taaattacgg tcaatggtta ccgcaatttc agcactccca atattcactg taccgaaaag
    31261 tggttcaatt ttcacccaat tcggcagttc gccggaaatt tcccattcca gcgcctcggt
    31321 accgctattg ctaatccgaa acgtttgagt atattcatca tgccagaacc acaaactatc
    31381 cggcgtaaga ataatccgcg gaggtatcac gattttcccc gaaacgatgg cacggatagc
    31441 ccaattgccg gtaagagaaa catcttccga accgccggtc acagtataat tgcagaggtc
    31501 gtgaaatatt tcatcagttt tgatataagc atgaccgaat ccgtcttgcg cagaactata
    31561 gccgagcgag ttacccgaac tggtaaccga taccgcaaat gaactcaaat cggccaagtc
    31621 aatcggattg ccgagaataa tctttgtcca agcctgttca tcaggaataa tgccactcat
    31681 agttaatagc ggcgcctccg ctaaattgct gtatattttt atcgtaaccg gactggaatc
    31741 ataggttaat attttaatcg ctgacagagt cgcttcagat aaatttaaat cgaacttctc
    31801 agcggcttcg gcatcctcta atcgaataaa ataatcgagc atatcattat cgtatcccaa
    31861 ttcggattcg gcatagcccc cgattccagt tgctgagtaa gatatattgg cttgaacagt
    31921 ggcggcaatc tcagctggcc aatagggaat aatagtcagt ttagcataat cacttccgaa
    31981 ggtctcatcg gaaaaattat aagttccggt gccattacag gtgacgaagg ttatttccgg
    32041 tgtctcataa tgtttaatca ctgccaatcc aaaactgtaa gacgattggt aattcatcac
    32101 aaactcgata ttttggcctg aataaaactg aatataaacc gccgccgccg gattgagcgc
    32161 caacgaaatc tgctcgcctt cggtaaaatt agaatgaaaa tagttcgatg atattggcga
    32221 aatcgccgcc ccaccataac catattgtcc atcacccatc gtcggatcgt taagaaggtt
    32281 ggcaacaaac caatcgcgta atagctccgc gaccgagcca tcgtaaccgg cagatgacaa
    32341 accaccgata aaaccgttaa atgagtcatt tttgttgtta aatatctttt taatcacatc
    32401 taatccaaag cgctgatata tataataagt ccataaaccg actttagcat aatcggctat
    32461 tgaaccatca aaatccatta acgaacgatt ggtctcgttt aaaaacgagg caaagctacg
    32521 tgccggtaaa cccagcaagc gtggcgccaa ttccgagaat ccttctgtta cccaaacatc
    32581 ctcatccgaa tcataaaaat tatgaatcaa atgctgaagt tcgtgtgcta cgattgagag
    32641 tgtacccaga tccttggcat ccgccggatt agtgtcaata taaataattt caccgtaatt
    32701 gcctttgcca ggcacctgat cgagcgggtc gaaatagccc gcaacgtagg tatcggattt
    32761 tcctgcctca taattatcac gtacgtcaat gagtaacaca aagagtttac cgttaccgtc
    32821 tttgtccggt aatgcaccga atatatcaat ctcgttgtaa taaatacctt tagcaggatt
    32881 aatcgaaccg ggcggcgtat cgtaaagcat tatatcgcgc acccgttcgg cgtcgctttg
    32941 agtaattcgt ccggcatcga attccgtttt ctcagcatat atttcaattt ctgcggtatt
    33001 aatcagacga aggaatgcaa tttcaataaa ctcagccgaa ttatcctcaa catttttccg
    33061 aataaagaaa gtagtatctc cggcaactgg tttggctaat ctatgatgaa aaacaatttc
    33121 cggatattta ttccataaaa atggcgtacc tgaaatcggt tggggtgcag gagtatggag
    33181 ttcctgcgcc attgtgacag tcacaaaaca gccggataaa agtagtaatc tttttagtga
    33241 agaaatcaat atttagagag tgagtgccgc tcctgtttag gatcgcggtt cattaaatcc
    33301 cacaaagccg tttgcgggtc tttattttta aaaagaactt tataaatttc ctccgaaatc
    33361 ggcattacga ctttgtactt ctcagccatc tggtgaaccg ttaaacaggt cgtgactcct
    33421 tcagccacca tcgacatacc attcagaata tcgtccagtt tgcggccttt gccaatttct
    33481 tcaccgacgt agcggttacg gctatgctga ctgttacaag taacaatcaa atcgccgata
    33541 ccacttaatc cggcaaaagt tacttctcga cccccgactc gcaatccgag acggacaatt
    33601 tccataattc cgcgcgtcat cagagcggct ttggcgttgt cacctaatct cattccgtcg
    33661 caaataccag ccgcaatcgc aatgacgttt ttgaccgccc cgccgatttc aactccgatg
    33721 atatcggtat tggtataaac gcggaaatat ggtgaatgaa atacttcctg aactagctgg
    33781 gcagtctctt catttatcga ggcggctaca accgccgtgg gaacttgccg ggcaacttct
    33841 tcggcatggc taggaccatg cagagtaact atcttttccg gtgggtgatt taaaatttca
    33901 gtaattaact cgctcatacg cttgagtgta ttttgttcga tgcctttggt aagattaacg
    33961 ataattgtat caggttcaat ctgccctttt attttctcca gcagggaacg catcgactgc
    34021 gacggcaccg ccagcaccag gaaattagca tttttcacgg cttcagtcag atcatttaaa
    34081 atcggaatcg cgcgcggtat cggaatgccc tccaaaagcg gattccgacg agtcttattc
    34141 atatacgcta cattttccgg aaaatattcc cagagtttga ccggcaattt tttattataa
    34201 agatggatgg caatcgttgt cccccaacta ccggcgccca atacggctat cgagatcgat
    34261 ttaggaattt ttcttagcat gaaaatatat tcagcaaacc tcaccgagaa taaccgcttt
    34321 ttctccggac ttttgtaata cctgagaaaa tttatcaacg gctgaagcgg cgcaaattat
    34381 aatcatcccg attccaaggt tgaaaacgcg gcgcatttct tctatatcaa tttcaccagt
    34441 tttttgaatg aatttaaaaa gcggtggcca ttcccaggct tgccagttga ttttcaaagt
    34501 caaattttcg gggacaatgc gggaagtatt accaatgatt ccgccaccag taatatgact
    34561 caacccatgt aaatctggat cagctagtaa tggctcaatc acatttaaat aggagcggtg
    34621 aattgccagc aaggcgtcgc cgatagtcgc atttaattcc ggaatgtaat cagtgacaga
    34681 atattttgtc aagagcacct tgcgagctaa agaataaccg ttggtgtgca aaccagtgga
    34741 aggtaaaccg agcagaacat cacctttccc aactcgacca attaacattt gctccctttc
    34801 caccactccg atgatcgttc ccaccaaatc ataatctttg gtttgataaa tatcaggcat
    34861 ctccgccatt tcaccgccaa ttaaggcgca accattggct ttacatgcca gagatattcc
    34921 cctaataatt tcagtagtta ttgctggttt tagctgacca gttccaatat aatccagaaa
    34981 aaacaaaggg cgcgccccag tcgtcaaaat atcattaaca caatgattta ccagacattg
    35041 cccgactgta ttatgccgat cgagcagatt ggcaactatt aattttgttc caactccgtc
    35101 ggtgctggct accaaaaccg gttgacggta attttttacc ggaaaagcaa aacagccgcc
    35161 gaataagcca atttcagtca atacattggg ggtaaaagtc tgttttacca gattcttaat
    35221 ttctttgaca gcttgttcac cggcggcgat gtcaacgccc gcatctttat atgatacact
    35281 cacttaactt tctgcctcct gatacattga ttcaattaaa tccgcataat gtttttcaac
    35341 taccgaacgt tttactttta gggacggtgt cagttccccg ctctcgattg taaattctga
    35401 tggtaataga acatattttt taattttctc gaattgggcg aagccttcca ttgattcggc
    35461 tatcactcgg tcatacaatt ccttgacagc tggaagggca attaactcct cccgactgtg
    35521 aaactgcaga ttgttctctt tagcccagtc ttccaaatta acaaaagccg gcaccactaa
    35581 agccgatacg aatttccgtc ggtcgccgat gactacaatt tgttcgatcc actttgaggt
    35641 aacgagcgca ttttccaccg gttggggagc aacattctta cctccggaag tcactaatat
    35701 gtttttctta cgatcggtaa tggtgatata gccatccccg tcaatcattc caatatcgcc
    35761 ggtatgaaac caaccgtccg catcgaggac atcacgggta gaggcttcat ctttataata
    35821 gcccagcata acgtgtgggc cgcgcgtcag gatttcgcca tccgaagcga ttttcacctc
    35881 gatattaggg agtttcggac caactgtccc gaccttacat tttgacagcg gattaacgga
    35941 aataactggt gatgtttccg ttaaaccgta gccttcgaga atcggaattc cagcggcttg
    36001 gaaaaattgg gcaatttcct tagagagagg tgctccgccg gaaacaaaaa accgcaaatt
    36061 gccgccgatt ttctcatata attttgaaaa aaccagtttt tgagcgattt ttagctggtt
    36121 atcgagaatt gcgccagtgt tccgattctc actctttaga aaaacggctt ttttaccgac
    36181 atttattgcc caccagaaga ttttctgact aattttggaa gatgttgaaa tactgtcaat
    36241 aactctagcg taaaccttct cgtaaaaacg cggaacggcg accatcaatg tgggatggac
    36301 ttccagcata ttctcggcaa ccgtgtcaat actctcagca taactgatac atgccccgga
    36361 accggtggct aaaaaatgtc ccgccatacg ttcaaaacca tgactgagtg gtaaaaacga
    36421 caaaaaagtg tcattttcat tagcctcaac gatttgtaat gcaccggtaa tattcgaagt
    36481 gagattgcca tgcgtcagca tagcaccttt gggttcgcca gtcgtaccag aagtataaat
    36541 tatagttagc aaatcgtctt ccttggtatt atgaatatct tcaaggaaag cactcggatt
    36601 ctggcctcga tattcggcac ccatttgcat caattcactc atcataatag ctttcgggtg
    36661 atcaattttc gtatcatcca ttataatgac tttataaact tcgggaagtt cgttaagaag
    36721 tggaatgact ttttcggtct gttcgcggtc accacaaaaa atgaaacgag aatcagaatg
    36781 ccgggcaatc caccacactt gtttagccgt aagagttggg taaatcgtca cgactacccc
    36841 tttggcacag atcacaccat aatcagtcat cgcccataat ggattgttag ccgcaataat
    36901 ggcgacatgt tcgccagctt tcatacccaa ggctttcagt ccgccggaaa tttgctcgac
    36961 acggttgcgc acttccgaaa aagtaagtcc cagccatttc ccatcctttt tatatttgta
    37021 cattggccga tcagtgtatt tatccgttat gcccacgaac atttcgctaa tagtactata
    37081 gctaaaatcc gacataatca actccctata tttacttttt tattgttttt aacttgtaaa
    37141 gagttaattt tagggcgctt tttgccgagg tggtgaaata ggtagacgca gtggactcaa
    37201 aatccaccgg acttcggtcc atgggggttc gattccccct ctcggcacaa aaaccacaat
    37261 catatatcaa agcaactccg catcaatgta atatttggat tcattgattt cactttaata
    37321 gctgataaaa aaatatttat ttaatcttgg ttaacgtcgc cggaataaat ctgaattgct
    37381 tttttggctt tggttacttc ctcaattgca ttcggataat ctcgtataac tttttcccag
    37441 gcacttacag ccagatctac ctgattcatt cggttatagc aatagcccat acgcagttga
    37501 gcataagcag atttattccc atcacccagc ccaaatactt tgcgatatgc atttaaggcg
    37561 ctgacataat cgccaagaaa ataatagcat tcaccgatcc accactgcgc attatcagcc
    37621 agcggatgtt gcggatctag attgataaat tcagtataaa cgctgatggc atcttcgtat
    37681 tttttctgtt gaaatagatc gttaccagtt tcataaagca tatcccgagt tctcgggcgg
    37741 ctttttggtg tttttgcctt ttctctttga acaggttttg gcgctgtact ggttacctct
    37801 ggttctggtg cttttccatg aaacacaaaa tccgctatag ttgggttgac cacttcacca
    37861 acgaataatt caattgacac ccggtgattc agcagttctt ttacttggtt atcactctcg
    37921 atcagcattt catcagcacc aacaggcacg atttcaatcc ggttggcaac gctatccttc
    37981 tcttcttctg gaaataaatc aagtattaat ttccccactg taatcgccct tttctgagat
    38041 tttatgatag cattaccgga tttcgtacca gtatccattg catagccttt caaccgaatt
    38101 ttgatatccg gatcactctt cagagctttc actactttgt taagagtaac tatataattg
    38161 gagttaaatg taaggttagg agtttctgtt tcatcggcat caaaaaatat atcatcaaaa
    38221 ataaggattt ttaaaacctt agccgaagtt tcggcttctt ttgtacataa acccggtttt
    38281 tccgtaacaa ccttactttc gagtactcca tttgtctcat tggaagtcgg tagcaaatca
    38341 gcatctattt tgatttcatc cgttataacg ggagagggtt gtatagctag ctcggaagta
    38401 ttaccagtca gaggagttga atccggctca ttttgaacat cctttttggc tggagtaaca
    38461 gaatccaatt gagactcttt cacggcggtt actgattgca taaccattcc agctgtatct
    38521 tttctaaccg tttcaaggtt agtagtctcg gttgaagttt ttggactttg tacctttttt
    38581 ggttctgttt tttcagttac tttggtttgt tcagtccttc tttctacttt cttagtttct
    38641 tttccaccag aacaagcagg caatgctaat gtggtggtaa aagctattaa aataataaat
    38701 aaatattttt tcacctctcc ctcctctaag attttaatgc ttaacaaatc caattatccc
    38761 cagatgataa aaatatatta tttatctatc agattatttt tttgctgggg taggtggagt
    38821 ttccaaggtc tttacacgtt tgtcgagatc actcaattca cgactcatcc gggtaaccgc
    38881 ggattgtaga tcagtaaggg tccgctgagt ttcacgttga aatttattga gtctttcgtt
    38941 aattccatcc tggtcggttt tcataacatt aaaacgagaa tcgatatcaa tttttaattc
    39001 agctatgctg gtgttagttt cttcgatttt gtcacttagc gccaagtcca cgctgtctaa
    39061 tcgtgaattc atatctttca gcgattgctg aaactgttga atatgtttgc ctctcccgac
    39121 aaattccaga ctcagatcat gtaaattatg atcaaaatcg ttgatggtta cactatgggt
    39181 ggaatcacgg gcatttaaaa tgtccaggcg tttgtttact ttttcaaatt caaagaaaaa
    39241 ataaacgacc gctacaacta caagcaccaa tgctatggtt ttaattagtt ctttcattca
    39301 tgtctcctgt acctttactt attgttaata ttttaaccga ggtgtttagc ccagtcatta
    39361 tcttcatttg ccgcaaaagg attaggttca gctggcgttt gaggtatatc agatattcct
    39421 ataatcggtc gatcagtact gccagtagaa tttgtcaatt ttagttcttt aattagattc
    39481 tcgacgtctt gatgaaaaat agcaatggtt ttttccattc tatgaaataa ttcttccatt
    39541 aactctttgc catagaggtt attgatacct tgaaataagt cgtcaaattt agttataaga
    39601 aaatttttac ggctttcctc aatgctaaaa tcgacttttt tatcttccat aattaacgct
    39661 gtattctttt taaaatatca atatttaatt tacaaatacc attttaatta tcaatatttt
    39721 tctatgtcca gactaccacc tttcttgctt taggtcttcc cagcgatcaa tttcccggat
    39781 tatttgacga caaacccctc gaataacact atttaattta atctcgactg aaggttgatt
    39841 agtagtcaac tgctgtaaga cttgacggaa ttccagtagt tcactcctct ccagctcaat
    39901 tgtcatacgg gttaacattt gatttcactc cttcttattt gaattta
//
LOCUS       DAOMJU010000010        36905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_939_length_36905_cov_22.707083, whole genome shotgun sequence.
ACCESSION   DAOMJU010000010 DAOMJU010000000
VERSION     DAOMJU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 36905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36905
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_939_length_36905_cov_22.707083"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..447
                     /locus_tag="PLO58_01875"
     CDS             73..447
                     /locus_tag="PLO58_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73569.1"
                     /translation="MKRSTLVRFAIFFSLTLVLIFFSGCATEAQRHYDLGKWYYEKGL
                     INEAILEFKAATQKDPDFYQAHQSLAIAYAKKGWYDYALKEAEISFDLHPSDEAYNLI
                     QIIRHKKILEPAFESALTDTLQ"
     gene            583..1419
                     /locus_tag="PLO58_01880"
     CDS             583..1419
                     /locus_tag="PLO58_01880"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HQO73570.1"
                     /translation="MKHILSVSRRSDIPAFYGEWFLQKVREGSVTLPSPFSRRIQVVS
                     LRPEDVSAIVFWSKNYQPFWPILKQIKPLYNGRFLFHLTINGFRSSAKILLEPGAPDT
                     EKALKTAHLLCDEYGPGKILWRFDPIIFSNLTPPEERLEAFSKLAEQLRGLTQRCYVS
                     FVDLYGKVKRRFNAAEASGKIQFNKPELPEQVAFLESLKPIATTAGIQLFTCCEDEAG
                     AAAGLPKGHCIDAELLKRIFPDEEFPTQVRPTRPQCGCYISRDIGAYRTCRHACLYCY
                     AQ"
     gene            1634..2491
                     /locus_tag="PLO58_01885"
     CDS             1634..2491
                     /locus_tag="PLO58_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HQO73571.1"
                     /translation="MSKTTKTSSRDSSRTLSRYLEEISHFSPLSPEKEIELARRIKNG
                     DRQAAKELTEANLRFVVSVAKDYQGQGLPLMDLINEGNLGLLKAVERFDETRGFKFIS
                     YAVWWIRQSILQALSEQSRLVRLPMNRVGTLTKIAREAEVLEKQNERSPRYDEIAAGL
                     DVDREVVNDTLHIARRHRSLDEPMGDSDSNSLIDVLTDENVTDPDWQLMVESLSHDIN
                     TSLAALNKREREVLKMYFGIGSEYALTLNEIGEHFNLTRERVRQIKERAIRRLRHKSH
                     SSLLKTHLG"
     gene            2690..4045
                     /gene="glmM"
                     /locus_tag="PLO58_01890"
     CDS             2690..4045
                     /gene="glmM"
                     /locus_tag="PLO58_01890"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQO73572.1"
                     /translation="MLIESLSGVRGYDTDLTKELISSYAAALKELLACRRVVLGRDTR
                     ASGVRIREQLTEALRRYGVAVIDLGICPTPTVEMAVVQHRADAGVVITASHNPLPWNG
                     LKFLGADGLFLDPEQMLWIKQNRQSLLAKYSDAHFSPGDLIFYEWANRDHINAILKLP
                     YVEIESIRRQHFKVAVDAVNGAGSKIIPELLKEFDCEVFAIHCDPYQPFPHPPEPLPE
                     NLTDLCQAVKDNSADLGLAIDPDGDRLAIVSDKGVPISEEYTLVLATKLVLSKTGKKN
                     QKVVANLSTTRALEDLAAQYDAEVIRTPVGEINVARRMQMEKALIGGEGNGGVILPEA
                     HLGRDSLVGTALVLQLLADEKRSLSAVMPTLPQYFMVKRKIPRGKADFNEIMNHLIQS
                     IDNAEINTEDGLKFSWSDRWVHIRTSNTEPIMRIYAEAPTSQAADQLADRFVTLLQKF
                     I"
     gene            4079..4780
                     /gene="deoC"
                     /locus_tag="PLO58_01895"
     CDS             4079..4780
                     /gene="deoC"
                     /locus_tag="PLO58_01895"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQO73573.1"
                     /translation="MTYASIINPEEARLAIAHLIDHTLLKPEATPNDIVRLCEEALKY
                     QFATVCVNPSFVELCANCLYNSKVAVATVIGFPLGANTMEIKAAEVEQALNHGATEFD
                     MVINIGRLKAGDLEYVEDDIHAVVDAADGNLVKVIIETALLTDEEKKLACHLALKAGA
                     HFVKTSTGFSKYGATVEDVRLIRSIVGNQMGVKAAGGIRTYSDAILMIEAGANRIGTS
                     AGVQIVQESKQFKNH"
     gene            4993..5865
                     /locus_tag="PLO58_01900"
     CDS             4993..5865
                     /locus_tag="PLO58_01900"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQO73574.1"
                     /translation="MGIPGLTIIGESINDSVPSTHDLFQANDLDGIVELARFQAEKGA
                     AYIDVNVGRRSPAFMAEVVQKIQQHVSVPLSIDTPDPEIALAGLEAYNPKLAGNRLPV
                     LNSISEARLEIFDLYSKQPFIPILLITEGKNESGEIVMNKTPEQNYATAKSLVEIARE
                     RIQGVANNRLILDPGIAPIGSDMEGNFKRLMKVMEMIHQDPDLAGINMSVGLSNFTVM
                     LPSKKSDGSPVKSPLESAFLTLAMPLGLNFIVGSVKRKYEILPDDHPAVQCLKNILVS
                     DGLNAVLQIMNFIA"
     gene            5907..6350
                     /locus_tag="PLO58_01905"
     CDS             5907..6350
                     /locus_tag="PLO58_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQO73575.1"
                     /translation="MDLVTFDKPPISARERKLSRFMLFFEDLIKVPVFHCQHCGECLL
                     SSTGFVCSQRCPKRLRNGPCGGTRDDGHCEVFPERKCIWYRIYRRSKLLGRIKLLYKI
                     NRIHNWNLEHTSAWLNVFRKRIEGLVLFVPKNLDIENINDTQRKN"
     gene            6328..7218
                     /locus_tag="PLO58_01910"
     CDS             6328..7218
                     /locus_tag="PLO58_01910"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQO73576.1"
                     /translation="MTLREKINSGKFIVCGEIGPPQSCDGDVIRRKAQYFKGIADAVN
                     ITDNQTAIPRLSSIASAKILLDEGVEPIIQMTCRDRNRLAIQSDLLGAAALGIRNVLC
                     LTGDYQKLGDNPEAKGVFDLDSIQLIATVAQMNSGYLLSGREMKKAPEFLIGGAANPF
                     AVPFEMRLIRLYKKVKAGVNFIQTQPVFDPELFIRWMEKVVEMGLHEKVAILAGVMPV
                     KSVNTLLHMREDVPGVKINDVYIQRMEKAADPQAEGIKIAVEIIQTVKNIKGIRGVHI
                     MPFMWESIMPTVVKEAGLCN"
     gene            7236..7901
                     /locus_tag="PLO58_01915"
     CDS             7236..7901
                     /locus_tag="PLO58_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HQO73577.1"
                     /translation="MQDIIEKIALCVEKGKISRNHPFPPDMKDQDGADEIAAAALKEG
                     VPPDVLLQASMLGMDRIGKKFSENLVFVPHLLVAAQAMNAVLKHLQPYLEAGSVKRKG
                     KFVIGTVAGDLHDIGKNLVAMIVRGGGFEVIDLGTDVSTQKFLSAVEQHPGCFVGLSA
                     LLTTTMLNMGKIVSAIRETFPTTKVLIGGAPVTEEFRAQIGADFYSPNPQQAVEYLNQ
                     FVS"
     gene            complement(7989..>8784)
                     /locus_tag="PLO58_01920"
     CDS             complement(7989..>8784)
                     /locus_tag="PLO58_01920"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HQO73578.1"
                     /translation="EIDSLLTIVSTQNLTITDRINLFSDKFLGMPYNQNCAGDGPYAL
                     YEPWPLVNFKETNCMVFCEHVLALSISDSWDNFFNNLQQIRYQDGIIGMRTRNHYTMA
                     DWLPENSWLLEDVSHQVGGQYIRQTTRTISHRKFFEDKGIADLRYVKPDRTITLEYLP
                     KEYLFKVENNICNGDILVLIFANKSDIFSAHMLISVVKNGKLFIRESSSRKMTTCETP
                     FTEWGKSIQSQDQYAGLCFIRIRAELNTPGRIILPWEITEMKKNHE"
     assembly_gap    8785..8884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8885..9746)
                     /locus_tag="PLO58_01925"
     CDS             complement(<8885..9746)
                     /locus_tag="PLO58_01925"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HQO73579.1"
                     /translation="MVKIKPFRGIRPVKELADKIAAPPYDVINFEEARSLAADNPYSF
                     LHVSRAEIDLPPETDPYDEKVYQKARENLEAMLAKGWFFQDATEMLYIYRQIMDKHSQ
                     YGLITTANVNDYLKGKIKIHELTRQDKEMDRTKHLEYTNVNTGPVFLTYQAKPVIDII
                     IHQIVTESQPEYDFTAVDGIQHTFWLVNDRETILKLVNHFSQLPHCYIADGHHRAKSA
                     ALVAKMRRERNPHHTGNEEYNWFLAAFFPHNQLRTLEYNRTVKDLNNLTEEEFLTKVG
                     EKFIIKPAECA"
     gene            complement(9755..10654)
                     /locus_tag="PLO58_01930"
     CDS             complement(9755..10654)
                     /locus_tag="PLO58_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HQO73580.1"
                     /translation="MKILIADGLDQKAQNILKEAGLELVVKHYEADDLIKVIQEFDGI
                     IVRSATKVPQVVIDAGKNLKFIARAGTGIDNIDHQYARAKGIAVLNAPGANSHSVAEL
                     VFAHLLALARFITPATISLREGRWEKKTFGNGIELAGKTIGIIGFGRIGKIVAHLAIA
                     WGMHVLVYDVATINTEPTVKVVDFETLLKESDFITLHTPKTDQPIINEKEIAMTKRGV
                     YIINCARGNAIDEKALLAGLNSGQVGGAGLDVFAAEPPQNMELLHHARVSCTPHIGAS
                     TLEAQERVGAEIAHRIVEYIGTL"
     gene            complement(10678..>11427)
                     /locus_tag="PLO58_01935"
     CDS             complement(10678..>11427)
                     /locus_tag="PLO58_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQO73581.1"
                     /translation="PTLPEVVDQYLSTGKYDVFTITHNETSTGLMNPVAEIAEVKRKY
                     PDVYWLVDAVSSMGGIKIPVDELGIDVCITSTQKCIGLPPGMAICSVSQRALEHARTV
                     PFRGAYLDLVELYDYLIKKDFQYPSTPSLSHMFALDLQLDYILNVEGLEKRYARHREM
                     AEITRAWAKENFAVFPQPGYESVTLTTITNTRGISIADLNKRLGEAGYQISNGYGELK
                     EKTFRIAHMAERTVDELKTLLNLIDTITAKM"
     assembly_gap    11428..11527
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11971..12999
                     /locus_tag="PLO58_01940"
     CDS             11971..12999
                     /locus_tag="PLO58_01940"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HQO73582.1"
                     /translation="MDPKTIEWSKLEFKYSYTPFRFQARWRDGHWDDGQLVTDKKISI
                     EEGSTCLHYGQEVFEGMKAQRTRDGRVMLFRPRDNAARLRRSAARLLMAEVPEDLFMR
                     GVIETVKANLDYVPPYGTGASLYIRPFQIGVGDNLGVKPAPEYLFSVFVCPVGPYFKS
                     GFQTIKVKVEEYYDRVARHGVGQAKAGGNYSASLLPLKIAHDEGYNELVYLDPVEHKY
                     FEEISSSNIFFVFKDGSLATPKSESILESITRLSLVRVAREEFGLNMEERRISIDEIE
                     NFAEAGACGTAAVITPIGSLGHRGKLYKFYADGEEAGPITTKLYKYLTAYQVGDVPDR
                     FGWLEEIK"
     gene            13017..>13258
                     /locus_tag="PLO58_01945"
     CDS             13017..>13258
                     /locus_tag="PLO58_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQO73583.1"
                     /translation="MTNDNFKWEDLDKDGLTYLMENRKEVLRNYQKMINSLGNHLDEK
                     TRHLIMLAIQINSPSPEAIKIILPKAIRAGATRDEI"
     assembly_gap    13259..13358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13359..13745
                     /locus_tag="PLO58_01950"
     CDS             <13359..13745
                     /locus_tag="PLO58_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73584.1"
                     /translation="GLVTDVLKTIIAGKPQPNFVLAVGPLPMMRAVSEVTRPHAIKTM
                     VSLNSIMIDGTGMCGGCRASVGGKTVFVCVDGPEFDGHQVNFEELAHRQKQFAAQEKI
                     SADILNTEPDKVLHQCRLSKVISENS"
     gene            13766..15229
                     /gene="gltA"
                     /locus_tag="PLO58_01955"
     CDS             13766..15229
                     /gene="gltA"
                     /locus_tag="PLO58_01955"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HQO73585.1"
                     /translation="MSSSNLSKKERLAIPRQQMPEQPPEERIHNFNEVNLGFDAETAV
                     REAQRCLECPHPTCIDGCPVSIKIPDFIRLIVQRDFSGAARKIKEDNLLPAVCGRVCL
                     QEEQCEKTCVIRGKFGPVSIGRLERFVADYERQNGQPQVSLSTPRNGKKVAIVGSGPA
                     GLACASDLIKWGYDVTVFEALHEFGGVLIYGIPEFRLPKAILKYEIDNLRKLGVRFEA
                     NFIVGKTMTIDELFQVAGFDAVFLGVGAGLPWFMNIPGENLMGVYSANEFLTRVNLMR
                     AYEFPEADTPIKIGKQVVVVGGGNTAMDAVRTARRLGAEKATIVYRRSEVEMPARLEE
                     IKHAKEEGVIFRTLTNPIEFLPDENGWVRAARCLQMELGEPDSDGRRRPIPIPGSEFE
                     IPTDLVIMAIGNGSNPLISRTTPDLQCNKWGNIITSGEGGRTSKRGVFAGGDIVTGGA
                     TVILAMGAGRAAAHSIDEYLKTGIWVTEPDSANKNKT"
     gene            15237..16421
                     /locus_tag="PLO58_01960"
     CDS             15237..16421
                     /locus_tag="PLO58_01960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQO73586.1"
                     /translation="MPISNYIAQSMTRSSWIRKMFETGIALKKQYGEDKIYDFSLGNP
                     DLPTPEIFNRVLVEESARTGTYVHGYMQNKGYPETCAAVANLLERESGLPFTEDQVVM
                     TCGAAGAINIILKSLLDPGDEVIVIAPFFSEYEFYVDNHRGQLVISEANAELLPDPAD
                     IAAKITPRTRVLLLNSPNNPTGRVYGEDIFREIGKLLTEKSAEFGRPIYLLMDEPYKR
                     IIFDGLQYRSPFRYYRHTILASSFSKDLSLAGERIGYLAISPLIEDYPALINAAAFCN
                     RVLGFINAPALMQRVIRRVLDAQVAIELYQKRRDMIYDALVKIGYEIIKPEGTFYLFP
                     KCPIDDDVKFVFALQDRLIITTPGIAFYRPGYFRISYAVPEAVIQAAIPIFKQVYNEM
                     KS"
     gene            16440..16679
                     /locus_tag="PLO58_01965"
                     /pseudo
     CDS             16440..16679
                     /locus_tag="PLO58_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            16841..17893
                     /locus_tag="PLO58_01970"
     CDS             16841..17893
                     /locus_tag="PLO58_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HQO73587.1"
                     /translation="MAKQFLKGNEAVVKGAVLAGCQAYYGYPITPASEIAHAAALYLP
                     VSGGTFLQAESEIAAINMVYGAAAAGQRVMTASSGPGISLKQEGISYCAGSELPCVIV
                     DVMRGGPGLGNIAPEQGDYNQVVKGGGHGNYKCIVLAPNSVQEMCDFGMLAFDLADKY
                     RNPVYILSDGFVGQMMEPVEFPEPVKQFPEKSWAIKGTPETSDNLITSIELDPDDLER
                     HNLKLQQKYAIIEEQEVRFEDYSLSDAEIVLVGFGIISRILQTIVDNLRDVGVKVGLL
                     RPITLFPFPKKKISQLAEQAKFFLVVEMSNGQLVDDVRLAVAGRKPVYLYNRLGGNVP
                     SEKEINDELLKLMRSV"
     gene            17890..>18959
                     /locus_tag="PLO58_01975"
     CDS             17890..>18959
                     /locus_tag="PLO58_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQO73588.1"
                     /translation="MNNILQKSAIFYDVFERKPGTDKLTTTYCPGCGHGNLHKLIAEA
                     LVDFGVQDRTIFISPVGCSVFAYYYFHTGNIQVAHGRAPAAATGLKRAHPEAIVISYQ
                     GDGDLAAIGTAEIIHAANRGEGITVFFVNNAIYGMTGGQMAPTTLIGQKTMTTPYGRN
                     ANNEGYPIGMSEIIATLKAPVYVERVMIGDTKSILHARQAVRKALKLQIEKNAFCFIE
                     VLSPCPSNLKMSAIRSREWVCEVLQKQFPLGVKKDISNEFPSRDLKRPTIDRSQLHSI
                     LNIEPITVDKEKIAPYKKTNFSAEIKVAGFGGQGILSLGLVLAEMGMRHDYEVSWLPS
                     YGPEIRGGTANCSVKISNRKIGS"
     assembly_gap    18960..19059
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19175..22027
                     /locus_tag="PLO58_01980"
     CDS             19175..22027
                     /locus_tag="PLO58_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73589.1"
                     /translation="MKFIFTTDRIFLRILLIAILSVFILVNSLLAQMLGSDQPPPDIH
                     WRQIETQHYRIIFPAEIESESQRMANTLEYVYLPLRKTLKANSPKWPLVLSNRSAVAN
                     GYVTLAPRKSEWFTTPDQSAMSGNIEWYNLLATHEGRHMVQFDRFNSGFTRLAGFLFG
                     EYAILGLSFWHAPMWFWEGDAVAMETALTESGRGCQPEFDLPIRTLLINQKLYSYDKA
                     YLGSYKDYYPNYYHLGFLMVTHGRRQYGANIWDHVLTSSTRRSWNPFGFSKACRINTG
                     RKTKELYYDTMTELDSLWCKPIEGLEITSAKKVNLKPKKVWTQYRWPQYATDGSIIAV
                     INGLGDINTLVKINPDGQETEIAKIASANRFSTAGNLIAWEELTPHPRWGAAIFSDIV
                     VYDLLVGQERKLTKNGKYFSPAVAPNGQMIAAVKFTPERKCNLVLLDSNDGQEIRTLS
                     NPDNGFIKNPAWSPDGKQLVYFKQSGSSKSLAIYDLENNTEEIIIAECHSFDEYPVFY
                     GDYLLYNSAWSGIDNIYAVEIDSGRRFQVTSRKYGAYYPAVSADGSMMLFSDYDFNGL
                     DVCAMHLEPARWQALEAVERCDIRYYETLVAQEQGGNIFEAGKIPTRSYPVSDYDPYR
                     HLFNFHSWYYFFSETSANLGLFSNDILNNAAANFELNFDANEKAFSVGSEVCYAGFYP
                     ILSAGFNLGKRISYSGGLFDELTTETWNETSFSLGANLPLDYSSSVFNRKLNLGLSGA
                     YTYISDKTTVEDYQIGNGGFVPFSYEIAYQNYQTPAIRDVKPTAGYTVKANFKHTPLG
                     GDYQSQMYSFQGNYYLPGLAKHHSLMLNAKVELQNPDNYRFASLVPFAKGYDYIYNDR
                     LAGAGATYELPLAYPDWALGSLFYLKRLRGAFFGDFNVGKTSDFEQQYNSVGLDLNFD
                     FNLLQLAVELNAGVRLAYRLAEKDLHASLLIFNIEF"
     gene            22094..>23770
                     /locus_tag="PLO58_01985"
     CDS             22094..>23770
                     /locus_tag="PLO58_01985"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQO73590.1"
                     /translation="MKPNDNINQVMIKIGGMHCAMCVKTVEEALGKVAGVRAVNVNLA
                     NEKAYLEFDDGHFSIEAAKRAIESVGYQYLGREGEQDLIEQDKTLNRELRSRKRRIII
                     GFITGVILMIPMFIKISLPFPIAYLMLALATPVFIYLSYPIFRAAGQALRNHSLNMDV
                     MYVMGIGVAFLASLLGTFEIVLTREFMFFETAIFLATFLTLGRYLETRAKGKTTAAIK
                     RLIGLQPQTVIVIRDNVESEIPLESVVIDDIVIVKPGGRVPVDGVVISGESYIDESML
                     TGESLPVFKQTGAKVVGGTLNQNGILHIRAERIGRDTVLAQIIRLVEQAQGSRPPVQR
                     LADVAVSYFIPVILAIAILSFGFWYFVAEQTLLFALTAFISVLVIACPCALGLATPTA
                     VTVGIGRGAELGILIKNGTVLEVAPRVTTVVFDKTGTLTTGKPLVTDIFPVGIATAEL
                     LRFAAAVEKNSEHPLSTAIVNYAKELGIAIPVSAKFAAFGGRGVTAYVDNRVIFSGTE
                     LFFQENKITISPDILAPAKKYTGEGKSIVFVAIDGQAVGVIAIADTLKDTA"
     assembly_gap    23771..23870
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23871..24465
                     /locus_tag="PLO58_01990"
     CDS             <23871..24465
                     /locus_tag="PLO58_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQO73591.1"
                     /translation="DGQAVGVIAIADTLKDTAASAIEKLCKDNLKIAMITGDNRRTAQ
                     AIAAQVGIETVLAEVLPQDKAAVIRQMQEKGEVVAFVGDGINDAVALAQADIGIAIGS
                     GSDVALESGDIVLVRNDLNDVSVALQLSRKVMARIRQNLFWAFAYNAALVPVAAGALY
                     PLFGITFRPELGGLAMALSSVTVISLSLNLRRFNPAR"
     gene            24528..25520
                     /locus_tag="PLO58_01995"
     CDS             24528..25520
                     /locus_tag="PLO58_01995"
                     /inference="COORDINATES: protein motif:HMM:NF017832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HQO73592.1"
                     /translation="MDLTQIKTIAAGFYIADENGKKLPGTIKIPYAEKDGFIRHISQV
                     EKGLACGCSCPVCHQPVIARKGAHKRHHFAHYPDAVCNAETVLHYLAKRLLFEKLQTL
                     LKQKVALVLRWKCQLCSDQHSFNFFENVQTVVSEEAFDDCRPDLCLVDQHDAPTALIE
                     IVVAHPPDEKVLIFVSRKQIPLLIFRIRSGEEMEEMATCPTINPDLIIHCPRRRCPEC
                     GAPLFEKSLYILPAVCWRCGAGMNLAFLDIDGQVAGTEEFSAKDRQIISRNNVILRDT
                     IRPGDRKRTVSHICPHCGVVTGNAYLRYLKRHEQRLLVQNSGKYCRQCHRHFDE"
     gene            complement(25588..28002)
                     /locus_tag="PLO58_02000"
     CDS             complement(25588..28002)
                     /locus_tag="PLO58_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQO73593.1"
                     /translation="MINGIISIIVLSLTTTSLPAQTITRPKIGLALSGGGAMGFGHIG
                     TLKLLDSLSIPVDYIAGTSMGGIIAALYAVGYTGAEIEEIARNVDWADLFNDAPSRQA
                     LPYFQKQEADKYQFELGIRDLRPVDKGGVIAGQKITLYMTRLVLPFLTVSDFDSLIIP
                     YRCVAVDLTTGSEVVIDHGSLPIAMRATMAIPSIFTPVVWGDSLLVDGGLLNNLPVNT
                     VRKMGADIVIASTVRSPFKTRDELRTTLDILSQTSNILRDNKLEAEAKNADLLINCQL
                     EKLGAADFSTAKVCKIIEAGEAAAHANLDELLDLKKKYNLSRYGPENINDLNSPSGYR
                     VARLIIKGSQTIPVDVVRSTIGIKVGELYSPVTLAAGVERLKSFDNYIRVRPTVEILS
                     DSTVEIKINLVEKVKAQINNIEVRGNEYLTDDFIRRSFGIIPGEVFSMERIESQINYL
                     YGLDYFNNISYTTEPAGEGKINLILNVVEHTPQKLRLGVRYDDFHYLVAALNFQTTSN
                     IVHGLRLDVEWQFIGLNQIKLKTLYPSRRFQLPFYPFVNAHYREIPTYVYSINGNRIA
                     SYIDRSALFGGGFGLLYKNYWNFEAELDYESINIKPDIAPDEPLRFYDWNDDLYKFQL
                     SSNIDLLDNAFMPRKGALVHCVYEKTISQIGRHTNYRRFELSADVYQSIKRNTLHFSG
                     FYGNAKMDGSTNRFIYRGGQETFVGVEYDQLVGSEMTILRLDYAYNLLHNLQAKAIVN
                     TAIGFRNIYHETIKESQNLLGYGIGLKYVSPLGPFDLVLGWGDKSVFQPGEIRAVIYF
                     RAGFLF"
     gene            complement(28074..29690)
                     /locus_tag="PLO58_02005"
     CDS             complement(28074..29690)
                     /locus_tag="PLO58_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73594.1"
                     /translation="MKLSQKSIINLLTFACLLLLNESGLTQDKELIDKVSGRMEYDFQ
                     QMTEESAVGDVNTLRQFIADSLKFVYGERYQQELDSLESIYMTTIKTLETECTRLNNL
                     NKILIDSLEKARSARPKSVIETKDLDPTFESRYFQYGKILRLEEPRHKTGFLKMPTVI
                     ENIAPFQIQELNEYYDRYFPIARSDSVLDFIIQINIREKDWGNAGCNIIKFLYLFPNS
                     ALGEEIKTVRAGIFRTEKYYQPYSAFLTDLLNKIPQLPLADQRYYHYVELLKGFPDQA
                     IRNNFIPEAYKFLELYPNSKYAAQANLWIAEALLANNQPHSAYLTLQKIMILYPSDEL
                     YCRALHTSGVIQQEQFNEYKNAVQTFTDLIKRFPQDTLAENAHYRIAKISDEYLNDWE
                     RAVEQYQIYADKYPQSPNAIPALMRKAKIQSTQMNLIEEAVRTYKSIDERYPSTTGAF
                     NALTEAGNLYMTKGRYDQAIEIYMSIFQKYPQSENAVAALEKVYDIYQTKIKDNEKSI
                     EILNLIINNYPDTKASARATKQLKKLEKAR"
     gene            29875..30804
                     /locus_tag="PLO58_02010"
     CDS             29875..30804
                     /locus_tag="PLO58_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HQO73595.1"
                     /translation="MPYLIPEPLKTGGTIGVIAPSSPAPRQTTALGLQYLQNLGYKVK
                     SASNLNRGKFFLAGSDAERLKYLEQFLCDPEIDALFCVRGGYGLIRIIDQLDYQRLAK
                     IKPKVLVGYSDITILQMALAAKLGWVTYSGPMVSTDLGQSLSSFSAEWLWRMITSNPY
                     PIELTNPLAQPLQVFRSGSAEGVLIGGCLSLITPLLGTAFMPDLKGAILIIEDVDEKT
                     YRLDKQLQILRLHGVFDQIAGLIVGQFVNCFPSNPKRSFTLEDYLSNALKDTHFPVIT
                     NCAYGHLKQRFTIPIGIRARISTNPPRLTLLGL"
     gene            30936..31151
                     /locus_tag="PLO58_02015"
     CDS             30936..31151
                     /locus_tag="PLO58_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQO73596.1"
                     /translation="MTKPEVQINPEWCKSCEICVKFCPRNVLEMGRFTAQVVHPENCN
                     GCKICESLCPDFAIVVTVPEKAKEEAK"
     gene            31148..32299
                     /locus_tag="PLO58_02020"
     CDS             31148..32299
                     /locus_tag="PLO58_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQO73597.1"
                     /translation="MNKSNASSSNSRIFMMQGNTACAEGAIAAGCRFFAGYPITPSTE
                     IAEVLARRLPQIGGNFIQMEDEIAAMGAVIGASLAGVKSMTATSGPGFSLKQENIGFA
                     IMAEIPCVIVDVQRGGPSTGLPTHVSQSDVMQARWGTHGDHSIIALCPSTVRETYDLT
                     IKAFNCAERYRTPVMIMTDEIVGHLSEKVELPDPASLEIVNRQRPTVPPSDYLPYQMT
                     ASDIPPMANFGEGYRYHVTGLAHDETGSPTNDPAQIEQQILRINRKIERYRDQIVEVE
                     EFETQDMEILVIAYGATARSAKKAVSLAREAGLKAGLLKLKTLWPFADIEIEALAKSV
                     NKIIVPELNLGQIAHEVEWTVRRAKPVIRVNRINGEPITPAEILAAIKN"
     gene            32308..33123
                     /locus_tag="PLO58_02025"
     CDS             32308..33123
                     /locus_tag="PLO58_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQO73598.1"
                     /translation="MDYLYYLRTENMPHIWCDGCGNGIVLKSLLRVINKLAWDKNDIA
                     LVSGIGCSSRTPGYVDFNTLHTTHGRALAFATGLKIANPKLHVIVVSGDGDATAIGGN
                     HFIHAARRNIDITMIIFNNMIYGMTGGQVSPTTPAGKMATTAPYGNPDPPFDISRLAA
                     AAGASYVARSTVFHYARLDKYIEQALTRKGFAVVEVLTPCPTSYGRMNKEGNGAAMLL
                     YQKENTVSLAKASGMSPEELRNKLVVGVFVDRQQPDYQSHYEAVVTKARSEIK"
     gene            33135..33695
                     /locus_tag="PLO58_02030"
     CDS             33135..33695
                     /locus_tag="PLO58_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQO73599.1"
                     /translation="MSFHYEIRLSGAGGQGLILVGKVLAEAAAIYDGLNATQSQSYGP
                     EARGGASRSEVIISDGDIDYPKAENIDLLLALTQEACDKYIADLKPDGILIVDSQMVT
                     KIPDGNFKVVALPIIQSAVERLGKFVVSNIIALGVIYKLAGIVSEDAIKNAIRARVPK
                     GTEELNLKAFQTGIELAEGWLKEHAR"
     gene            33720..36728
                     /locus_tag="PLO58_02035"
     CDS             33720..36728
                     /locus_tag="PLO58_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73600.1"
                     /translation="MKSAIHYSLTDLLNRASDLGNHHRKFIHNIEINFDNLFALINNE
                     HLTDQFHLLRFSPHPAREMKLIADLVANRETLLEMLGCYFSIQLLLLNFKAIDVLKMK
                     LSTTTNHYAVYRSFLLRTGNDFRLLTAAYMNTLLDIFLPRAQRPDFVICGVGTRVDQD
                     DIDLGIVDTGPANRAILTNAFGAMSNEMLKYACSLHFHLSEHVGTKGYTASIEDYHNL
                     LDHQIQDFVLISEMVNAVPILGNRELFDRFKNEILDRYYFSADRDNKYHEGFLRGLLG
                     EIRDLMYKEPPENLLNPKRDGLRMLKALIFALKTWKRVEGNTSLEVLDILLVKERFNH
                     ENYYQVFQALTFLETFRFLYQLFVVQEEEIHLNSDEMQLSLQKVAYAMGYEDHSYALA
                     GTQMLTHYYDHHKIARLGTEHLLEGLTEHLRQITIFYPITHFQSRAESKAPLPDNIPL
                     NFMQTARFFQGVRFWDDLLSSLDNPNQILLKRFLADFMKLSEARRAVLMKAYVKWGVK
                     TPYTMISLFSIIADHQPKIVETQFFQEFIRDFIYNLEPTLENVSRLCQILNLAPQMMN
                     NFLSILPVELLTRLIAVVEEPYWKPEITSLRDTLLMLCRIYRSSSYYFKRFVQRAFNT
                     YAEYITSLTNQPKLNQLAEGLFRNIDNFDSLNEKITRLGDYYDFEFMRLGIALLDGAP
                     FDKINREFTNFSDNYLQMLFELCQEEVAAADQKEIPEMHDLLAVFTAGGHARGQAFDD
                     DYDLIILLNSDDPAIIDFSNKVIQKMNKYILRRSIIPQFRFADRFRRFVTTFSDLRRL
                     FERPDENTFIDQSQLLGARLVIGSDYFLKVFQSEIITPFIFQRKAEFIEAMLNEMRSR
                     HNYYNSTKIEVPEIKEGPGGLRDFENFIFILKAYFEWAEPLTETLFSRIESAITNDDS
                     NFISLVRDYQLLKHIRDLYRLSVSNDDHLDKAYLDQLVRPFCLAHGLELENGAELAEF
                     IQRIMRKNVERINLLLRTLELPEIGALPAK"
BASE COUNT         9868 a         7882 c         8439 g        10216 t
ORIGIN      
        1 tttttacctt gtttttatgc ctttataatt gaaaattaag cgtaaatgac gagtaattcc
       61 aggagaaata atatgaagcg ttccaccctt gtccggtttg ctattttctt ttctttgacc
      121 ttagtgttaa tatttttctc cgggtgcgcg acggaagcgc agagacatta cgatctcggc
      181 aaatggtatt acgaaaaagg cttgatcaat gaagccattc tcgaattcaa agccgctacc
      241 cagaaggatc ccgattttta ccaagcccat cagagcctgg cgatcgccta cgccaaaaag
      301 ggctggtacg attacgctct gaaagaagcc gaaatatcct tcgatttaca tccatcggat
      361 gaagcctaca atcttatcca gattatccga cataagaaaa ttctcgaacc ggctttcgag
      421 tcagccttaa ctgatactct tcaatagtag attaaaacca cgactattta ctaccgggcg
      481 aggagcaatt gcttccggtc gttaaatttg cgtcattttg aaagggagga tttttaaaat
      541 tttatttcgt attctttgcg atctttgcgg taatttatac cgatgaagca tattctatcc
      601 gtcagtcggc gtagtgatat tccagctttt tatggcgagt ggtttttaca aaaagtccgc
      661 gaaggcagtg ttaccttgcc cagcccgttt tcccgccgaa tccaggtggt ttctctgcgt
      721 cccgaggatg tcagcgcgat agtattctgg tcgaaaaatt accagccctt ttggccgatt
      781 cttaagcaga ttaagccgct ttataatggt cgttttctgt ttcatttgac gattaatggt
      841 ttccgcagtt cagcaaaaat cctgctggaa cccggtgcgc ctgatactga aaaggctttg
      901 aaaacagctc atttattgtg tgatgaatat ggtccgggaa aaattttatg gcgtttcgat
      961 cccatcattt tttcgaatct aacaccgccg gaagaacgtc tggaggcttt cagtaaatta
     1021 gccgagcagt tacgggggct gactcagcgt tgttacgtga gtttcgttga tctctatggc
     1081 aaagtcaaac gccgttttaa cgccgcagaa gcttccggca aaatccagtt taataagccg
     1141 gaactgcccg agcaagtcgc ctttctcgaa agtttgaaac caattgccac cacagccgga
     1201 atccagctat ttacctgttg tgaagatgag gctggcgcgg cggctggttt gccgaaaggc
     1261 cattgtatcg acgccgaatt actgaagcgc atttttcccg atgaggaatt tcctactcaa
     1321 gtgcgaccca ctcgaccgca atgcggttgc tatatcagtc gtgacattgg agcctaccgc
     1381 acctgccgtc acgcctgcct ttattgctat gcacaatgag tggaacaaat atcacttttc
     1441 attatttaat gggtagtttt ttgattgatg aatgaataga tatttttggt aattatggga
     1501 aaatttttga gatgattata gaaaagaaaa tgcccattaa tttcgaaaat atagaaatta
     1561 aatgcgaatg taattgacaa aatagaaaaa taaccttatt tttaatcttg aatgaaaaat
     1621 taaaaagtag aaaatgagta agacaaccaa aacctccagc cgcgattcca gccgaacctt
     1681 gagtcggtat ctggaagaaa tcagccattt ttcaccgttg tcgcctgaga aggaaatcga
     1741 gttggcgcgg cggatcaaga acggtgatcg tcaagcggct aaggaattga ctgaagccaa
     1801 tttaagattt gttgtcagcg tagctaagga ctaccagggg caaggattac ctctgatgga
     1861 tttaattaac gaaggcaatc ttggcttgtt aaaagccgtt gagcgtttcg atgagacccg
     1921 cggtttcaaa tttatttcct atgccgtttg gtggatcagg caatcaattc tgcaagcgct
     1981 ttctgaacaa tcgcgtttag tgcggctacc gatgaatcgg gtcggcactc tcaccaaaat
     2041 tgcccgggaa gccgaagtgc tcgagaaaca aaatgaacgc tcaccgcgct atgatgaaat
     2101 tgccgccggt ttggacgttg atcgcgaagt tgtgaacgac accctccata tcgcgcgccg
     2161 tcaccgctcc ctggatgaac ctatgggcga ttctgatagc aatagtctga ttgatgtact
     2221 gacggatgag aatgttaccg atccggattg gcagttgatg gtagaatcgc taagccacga
     2281 tatcaatacc tcgttagccg ccttgaataa acgtgagcga gaggtgttga aaatgtattt
     2341 cggaattggc agtgaatatg cgctgacttt gaacgaaatt ggtgaacatt ttaatctgac
     2401 gcgtgaaaga gtacgccaga ttaaggagcg agctattcgt cggcttagac ataaatcgca
     2461 tagttcgtta ttgaaaacac atctgggtta attctgtttt caatataaga ttagaaaaac
     2521 agtttctgtt ggtgagtaga ggggaaatta tttatccaga aagaagtata ataggtttaa
     2581 gactggcaac ttgtgaactt gccggattga tactatatta ttaaaagtat gcttgattat
     2641 tacctagtta aaaatataaa aatacctgac agagcagaag gaatcgaaaa tgttgattga
     2701 aagtctatcg ggggtgcgcg gttatgatac ggacttgacc aaggaactaa tatcgtcgta
     2761 tgccgccgcc ctaaaggaat tgctcgcctg ccgaagagtc gtccttggac gtgatacgcg
     2821 cgccagcggc gttcgcatcc gggaacaatt aactgaggct ctgcggcgct atggggttgc
     2881 ggtaattgac ctgggaattt gtcctacacc gacggtcgaa atggctgtag ttcaacatcg
     2941 agccgatgcc ggcgtcgtca taactgccag tcacaatcct ttgccctgga atggattgaa
     3001 atttctggga gccgatgggc tttttcttga tcccgagcag atgctctgga ttaaacaaaa
     3061 ccgccaaagt cttttagcga aatacagtga tgcccatttt agtccgggcg atttgatatt
     3121 ttatgagtgg gcaaatcgtg accatattaa tgctatcttg aaattaccgt atgtcgagat
     3181 agaatctatt cgtcgtcaac attttaaagt tgccgtggat gccgtcaacg gtgcgggttc
     3241 caaaataatt ccggaattac ttaaagaatt tgattgcgaa gtatttgcga ttcactgtga
     3301 tccctatcag ccttttccgc atccgccaga acccttgccg gagaatctga ccgatttgtg
     3361 ccaagcggta aaggacaact cggctgatct aggcttagca attgatccgg acggtgaccg
     3421 cttagctatt gtctcggaca aaggtgtgcc gatttccgaa gaatatacct tggtgctcgc
     3481 gacaaaactg gtattgtcaa agaccggcaa gaagaaccaa aaggttgtcg ctaacctttc
     3541 gactactcga gcgttggagg atcttgccgc tcaatatgat gctgaggtaa ttcgtactcc
     3601 ggtcggcgaa atcaacgttg cccggcgtat gcagatggaa aaggctttaa tcggcggtga
     3661 aggcaacggc ggggtaattt taccggaagc tcatctggga cgtgattcgc tggtaggaac
     3721 agctttagtt ttgcaattgc tggcggatga aaagcgctca ctttcagcgg tgatgccgac
     3781 tctgccgcaa tattttatgg taaagcgtaa aattcctcgc ggaaaagccg attttaacga
     3841 aattatgaat catctgattc agtctattga caatgccgaa attaacacag aagacggttt
     3901 gaaatttagc tggtcggatc gctgggtgca tatccgcacg tccaataccg aaccgattat
     3961 gcggatttac gccgaagcgc caaccagtca ggcggctgat caattggctg atcgtttcgt
     4021 cacattattg cagaaattta tttaaagcta atatatttaa attaataagg aaattatcat
     4081 gacttatgcc tcgataatta atcccgaaga agcgcgcctc gctatcgcgc atttaataga
     4141 ccatactttg ttgaaaccgg aagcgacgcc gaacgatatc gttcgccttt gcgaagaagc
     4201 tctgaaatat cagtttgcaa ccgtatgcgt caacccatcc ttcgtggaat tgtgtgccaa
     4261 ttgcctctat aattctaaag tcgctgtagc gaccgtaatc ggctttccgc tgggtgctaa
     4321 tacgatggaa atcaaagccg ctgaagtgga gcaagcgcta aatcatggcg ccaccgagtt
     4381 cgatatggtc atcaatatcg gtcgcctgaa agccggtgat ttggaatacg tcgaagatga
     4441 cattcatgcc gttgtggacg cggctgatgg taatttggtg aaagtgatta tcgaaaccgc
     4501 acttctaact gacgaagaaa aaaaactggc ttgtcatctg gcgctcaaag ccggtgctca
     4561 ctttgtaaaa acttcgaccg gtttcagtaa atacggggca acagtcgaag atgtgcgcct
     4621 gatacgaagc attgtcggta accagatggg agtcaaagcg gctggcggca ttcgcactta
     4681 ctccgatgct atattgatga tcgaagcggg cgccaaccgc atcggaacca gcgccggggt
     4741 acagatagtt caggaaagta aacagtttaa gaatcattga atcttcgacg atgagcatca
     4801 gattatttga gtcggtgcgg taagattgga atgagagcag ggagggcttt ataatgttcc
     4861 taaatacaac gaagctccca ttctttgaat aaaattctct gtaacgaaca cagtaattaa
     4921 tccttggaaa aaacagttga aatgttgaaa atagcggcta tgaatttact ataaaatgaa
     4981 aggtctttat aaatgggtat cccaggactg actattatcg gtgaatcaat taacgattcg
     5041 gtgccttcga ctcacgatct ttttcaggcg aacgacctgg atggtattgt tgaactggct
     5101 cgtttccagg cagaaaaagg agcggcttat attgacgtga atgtcggtcg gcgctcgcct
     5161 gcttttatgg cggaagtcgt ccaaaaaatc caacagcacg tttccgttcc tttatcaatt
     5221 gatacgccag atccggaaat cgctctcgca ggtctggaag cctacaatcc taaactggct
     5281 ggtaatcgtt tgccagtcct gaattcaatt tcagaagctc gtctggaaat ttttgattta
     5341 tactccaaac agcccttcat tcccatttta ctcattactg aaggaaaaaa tgagtcgggc
     5401 gaaatcgtta tgaataaaac acccgaacag aattatgcca cggcaaaatc tttagtagaa
     5461 attgcccggg aaaggattca gggagtagcc aataatcgcc ttatcctcga tccgggcatt
     5521 gccccgatcg gcagtgatat ggagggcaat ttcaagagac tgatgaaagt aatggaaatg
     5581 attcatcagg atcctgacct ggctggtatt aatatgtcgg ttggtttgag caactttacc
     5641 gttatgttgc cctcaaaaaa gtccgacggc tcgccggtga aaagtccgct ggaaagcgct
     5701 tttttgacac tggcgatgcc gctgggtttg aattttatcg tcggttcggt aaaacgtaaa
     5761 tacgagattc ttccggatga ccatccggcg gtacaatgtt tgaaaaatat tctggtatcc
     5821 gacggattaa atgccgtctt gcaaattatg aactttatag cctgattctt aataaccgtt
     5881 aagtcgaatt ttcaaaaagg gaaaatatgg atttagtaac tttcgataaa ccgccaatat
     5941 ctgctcgcga gcgcaagtta tcacgcttca tgctcttttt tgaagacctt ataaaagtcc
     6001 ccgtatttca ttgtcagcat tgcggcgaat gtctgctttc gtcaactggg tttgtctgtt
     6061 ctcaacgctg tcccaagcga ctgcgcaatg gtccgtgcgg cggcacgcga gatgacggcc
     6121 actgtgaggt ttttcccgaa cgcaaatgta tctggtatcg gatataccga cgatcaaagt
     6181 tattagggcg catcaaactt ttgtataaaa ttaatcgaat tcacaactgg aatctggaac
     6241 atacttccgc ctggctgaat gtatttagaa aacgcatcga aggcttggtt ctatttgttc
     6301 ctaagaattt agatatagag aatattaatg acactcagag aaaaaattaa ttccggaaaa
     6361 tttatcgtgt gtggcgagat tggtccgccg cagagctgtg acggcgatgt gattcggaga
     6421 aaagctcagt atttcaaggg aatcgccgat gctgtcaata ttaccgataa ccaaactgcc
     6481 atcccgcgtc tgtcgagcat tgcttcggct aaaatattgc tggatgaggg agttgaaccg
     6541 attattcaaa tgacctgccg tgaccgcaac cgattggcga ttcaaagcga cctgctgggg
     6601 gcggcggctc tcggtattcg aaatgtgctg tgcctgaccg gcgattacca aaaattaggc
     6661 gataacccag aagccaaggg cgtattcgat ctagattcga ttcaactgat agccactgta
     6721 gcccaaatga attcaggtta tttactctcc ggtcgtgaaa tgaaaaaagc gccggaattt
     6781 ttgattggcg gggcggcaaa tccttttgcc gtgccttttg aaatgcgtct gattcgtctt
     6841 tataaaaaag tcaaagccgg tgtaaacttt attcaaaccc agccggtttt cgatccggag
     6901 ctcttcattc gctggatgga aaaagtagtg gaaatgggat tgcatgaaaa agtcgctatc
     6961 ctagctggtg ttatgccagt aaaatctgtc aatactttac tgcatatgcg agaagacgtt
     7021 cccggcgtta aaattaatga tgtatatatt caacggatgg aaaaagccgc cgatccgcag
     7081 gctgaaggca ttaaaattgc cgtagaaatt atccagacgg tcaaaaatat caaggggatt
     7141 cgcggagttc atattatgcc ttttatgtgg gaaagtatta tgccaactgt agtgaaagag
     7201 gctggtctat gcaattaaaa ctaaggagtt agataatgca ggatattatt gagaaaatcg
     7261 cgctctgtgt cgaaaagggg aaaatttcac gcaatcaccc ctttccgcca gatatgaaag
     7321 atcaagacgg cgctgatgaa attgccgccg cggcgttgaa agaaggcgtt ccgccggatg
     7381 ttttactgca ggcttctatg ctgggaatgg atcggattgg gaaaaaattc agcgaaaatc
     7441 tggtttttgt gccgcatttg ctggttgccg ctcaggcaat gaatgccgtc ttgaaacatt
     7501 tgcaacctta tctggaagcc ggttcggtta agcgcaaagg taaatttgta atcggcaccg
     7561 ttgctggcga tttgcacgac atcggtaaaa atctggtagc tatgatcgta agaggcggcg
     7621 gttttgaggt tattgatctg ggcaccgatg tttccactca aaagttttta agtgctgttg
     7681 aacaacaccc cggttgtttt gttggactta gcgccttact aaccactacc atgttaaata
     7741 tgggaaaaat tgtgtcggca atcagagaaa cttttccgac tactaaggta ttaattggag
     7801 gcgcaccggt taccgaagaa ttccgtgccc aaatcggagc ggatttttac tcacccaatc
     7861 cccaacaggc ggtcgagtat ctgaatcagt ttgtgagctg attttcataa cgtttaatta
     7921 ttcaccttgt ccattttatt atccgtcagt attcgacggg ggtataagta tcaatgggct
     7981 ttgtaatctt attcgtgatt tttcttcatc tcggtgattt cccacggcaa tatgatgcgg
     8041 ccgggtgtgt tcaattcggc gcgaatccgt ataaaacata atccagcgta ttggtcttgg
     8101 gattgaatgg atttgcccca ttccgtaaat ggggtttcgc aagtagtcat tttacggctg
     8161 gaagattcgc gaatgaacag ttttccattt ttaactactg agattagcat atgcgctgaa
     8221 aaaatgtcgc ttttgttggc aaaaattaac accagaatat cgccgttgca gatgttattt
     8281 tcgactttaa acagatattc cttgggcaaa tattcgaggg taatagtacg gtcaggcttg
     8341 acatagcgca aatcggcaat gcctttatcc tcgaaaaatt tgcgatgtga aattgtgcga
     8401 gtcgtttgtc gaatgtattg tccacccact tgatgtgaga cgtcttccaa taaccagctg
     8461 ttttccggta accaatcagc cattgtataa tgattacggg tgcgcatgcc gataatgcca
     8521 tcctgatacc gaatttgctg gagattattg aaaaaattgt cccagctatc agaaattgag
     8581 agcgccaata catgttcaca aaaaaccata caattagttt ctttgaaatt aactaatggc
     8641 cagggttcat aaagggcata agggccatcg ccggcgcaat tttgattgta tggcataccg
     8701 agaaatttat cagagaagag atttatgcga tcagtaattg ttaggttttg ggtgctgaca
     8761 attgttagga ggctatcaat ttctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnntggcac attcggccgg tttgataatg aatttctctc cgactttcgt gagaaattcc
     8941 tcttcagtca aattatttaa atcctttaca gtgcgattat attcgagagt acgaagctga
     9001 ttatgtggga aaaaggcggc taaaaaccag ttatattctt cattgccagt gtggtgcgga
     9061 ttgcgctcgc gccgcatctt tgcgactaaa gcggctgatt tggcgcgatg atgaccgtca
     9121 gcaatatagc aatgggggag ctggctgaaa tggttgacta acttgaggat ggtttcgcgg
     9181 tcgttgacca gccaaaatgt atgctggatt ccatccacgg cagtaaaatc atattccggc
     9241 tggctttcag ttacaatttg gtggataatg atgtcaatga ccggtttagc ctggtaagtc
     9301 aaaaataccg gaccggtatt gacattagta tattcgagat gcttggtccg atccatttcc
     9361 ttatcctggc gagtcagttc gtggattttg atttttccct taagatagtc attgacgtta
     9421 gcggtggtaa tcaatccata ctggctatgt ttatccatta tttggcgata gatatatagc
     9481 atttcggtcg catcctgaaa gaaccagcct ttggcaagca ttgcctctaa attttcgcga
     9541 gctttctgat agactttttc atcataggga tcggtttccg gtggtaaatc tatctccgct
     9601 ctgctgacat gcaggaaaga atatggattg tcggcggcaa gacttcgggc ttcttcaaaa
     9661 tttatgacat cataaggcgg agcggcgatt ttgtccgcta attctttaac cggccggatg
     9721 ccacggaatg gtttgatttt taccatataa attcctacag ggttccgatg tactccacga
     9781 ttcggtgagc gatttcggcg ccgacgcgtt cctgagcttc cagagtgctc gcgccaatat
     9841 ggggagtgca ggagacacgc gcatggtgaa gtaattccat gttttgcggt ggttctgcgg
     9901 caaaaacatc cagaccggcg ccgccgactt gaccagaatt caaacctgct aacagtgcct
     9961 tttcatcaat agcattgccg cgggcacaat taataatata gacgccacgc ttcgtcatag
    10021 cgatctcttt ttcgtttatg atcggttgat cggttttggg agtatggaga gtaatgaaat
    10081 ccgattcctt tagtaatgtt tcaaaatcga ctacctttac ggtaggttcg gtattgattg
    10141 ttgcgacgtc atatacaaga acgtgcattc cccaggcaat tgctaagtga gctacgattt
    10201 taccgatgcg tccgaaacca attatcccga tcgttttgcc ggctaattca atgccattac
    10261 cgaaagtttt cttttcccag cgaccttcgc gtaaggaaat cgtggcggga gtgatgaaac
    10321 gtgccagagc aagcaggtgg gcaaagacta attctgctac tgaatgcgag ttagcaccgg
    10381 gtgcattcag aacggcgatt cctttagccc gagcatattg atgatcaata ttgtctatac
    10441 ctgtgccggc ccgggcaatg aatttaaggt ttttaccagc gtcaatcaca acttggggta
    10501 ctttggtagc tgagcgcaca attataccat caaattcttg aataactttg atcagatcgt
    10561 cggcttcgta atgtttgaca accagttcca gtccggcttc ttttagaata ttttgggctt
    10621 tttgatccag accgtcggca attaatattt tcatactttt ctttctggcc aagaatttta
    10681 catcttagcg gttatcgtgt caatgaggtt caataaagtc tttaactcat caacggtgcg
    10741 ttcagccata tgggcgatgc ggaaggtctt ttctttcagt tcgccgtagc cattggaaat
    10801 ctggtaaccg gcttcaccaa gccgcttgtt caaatcggct attgaaatac cacgggtatt
    10861 ggtaatggtg gtcaaggtaa ctgattcgta gccgggttgc ggaaagacgg caaagttttc
    10921 cttagcccag gcgcgcgtaa tttcagccat ttctcgatga cgggcataac gcttttccag
    10981 accctcgaca ttcagaatat agtccaactg taaatccaga gcgaacatgt gcgacagaga
    11041 aggagtagac gggtattgaa aatctttttt gatcaaataa tcatagagct cgacaagatc
    11101 gagataagcc ccgcgaaacg gtacggtacg cgcatgttcc agcgctcgct gactaaccga
    11161 acagattgcc attccgggcg gcagaccaat acatttctgc gtcgaggtaa tgcaaacgtc
    11221 aataccgagt tcatctaccg gaattttaat gccacccata gaagaaaccg catccactaa
    11281 ccagtaaaca tcgggatatt tgcgtttgac ttcggcgatt tcagctaccg gattcatcaa
    11341 accggtcgag gtttcgttgt gagtaatcgt gaaaacatcg tatttaccgg tcgagagata
    11401 ctgatcaact acttctggca aagtaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnact tcaggccgga catcaactgg gcctggaata aataattttt tatacattac
    11581 gacctcctac ggtgatttag ttcaaaactt taaaactgct ttgcattgag ctagtaggtt
    11641 cgcaaattca taaccaaatt ttaatacttc ataaaaatct ctggtgaata tacgtagaaa
    11701 tcggaaaatc tccgtaaata ttcattccta aaactaataa atcgccggct ggttgttgcc
    11761 agttttagga aaaatataga ctttaggatt atgctattta ttctccaaat caaatgaaac
    11821 cagcctttac ctgctagatt agcgaattta tactgactgg tggcattaca tttgataaaa
    11881 aaattaagga taagtgtttt ttgaaggtta aattaactaa agcaagccgt tcaggtttgc
    11941 taataaaatg gattaaaaaa gaggaaaatg atggacccga aaacaatcga atggagtaaa
    12001 cttgagttca aatattccta cacgcccttt cgttttcagg cgcgttggag agatggtcat
    12061 tgggatgatg gccaattagt aactgataaa aaaatcagca ttgaggaagg ttctacctgt
    12121 ttgcactatg ggcaggaagt ttttgaaggt atgaaggctc agcggacccg tgatggacgg
    12181 gttatgcttt ttcgcccgcg ggataacgcc gcgcgtctcc ggcggtcagc cgcccgccta
    12241 ctaatggctg aggtgccgga agatttattt atgaggggcg taatcgaaac ggtcaaggct
    12301 aacctggatt atgtcccgcc ttatggcact ggcgcatctt tatatattcg tccgtttcaa
    12361 atcggggttg gcgataatct cggagtcaaa ccggcgcctg aatatctctt ttcagttttc
    12421 gtttgcccgg ttggtcccta tttcaaatcc ggattccaga ctattaaggt gaaagtcgaa
    12481 gagtattatg atcgggtagc ccggcacggt gtcggtcagg ctaaagccgg tggtaattat
    12541 tcggcatcgc tcttgccact aaaaattgct catgatgaag gctataatga gcttgtttat
    12601 ctcgatcccg tcgaacataa atatttcgaa gaaatcagtt cgtcaaatat tttcttcgtt
    12661 tttaaagatg gcagtttagc aacccccaag tcggaatcaa ttctggaaag tattacccgt
    12721 ctgtcattag ttagggtagc acgcgaagaa tttggcctga atatggagga gcgccgcatc
    12781 agtattgatg aaattgaaaa cttcgccgag gcgggtgcct gtggaacggc ggcggtcatc
    12841 acaccgatcg gcagtctcgg gcatcgcggc aagctctata aattttatgc cgacggcgaa
    12901 gaagccggtc caatcacgac taaactgtat aaatatctaa cagcctatca agttggtgac
    12961 gtccccgata gattcggctg gctggaagaa attaaataaa ttggcagagg attgaaatga
    13021 cgaatgataa tttcaaatgg gaagatcttg ataaagatgg actgacttat ttgatggaga
    13081 atcgtaagga agttttacga aattaccaga aaatgattaa ttcactcgga aatcacctcg
    13141 atgaaaaaac ccgtcatctg ataatgctgg cgatccagat caacagcccg tcgccagaag
    13201 ccattaaaat cattttgcca aaagcgattc gagccggcgc tacccgcgat gaaataatnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg tttagttacg gatgtattaa
    13381 aaaccattat agctggtaaa cctcaaccga attttgtgct ggcggtcgga ccgctcccaa
    13441 tgatgcgagc cgtatcggaa gtaacccgtc cccacgccat caagacaatg gtatctctca
    13501 atagtataat gattgacggt accggaatgt gtggcggttg ccgggcgtca gtcggcggca
    13561 agactgtttt cgtgtgtgta gatggtccgg aatttgacgg tcatcaggtt aattttgaag
    13621 aacttgctca ccgtcagaaa caatttgccg cccaggaaaa aatctccgcc gatattttga
    13681 ataccgaacc ggataaagtt cttcatcaat gccgattatc caaagtcatt agcgaaaata
    13741 gttagttctg aaaaggaaaa agtcagtgtc atcgtcaaat ctcagcaaaa aagaacgatt
    13801 agcaattccc cgtcagcaaa tgccggaaca accacccgaa gaacgtatcc ataatttcaa
    13861 tgaagttaat ctcggcttcg acgctgagac ggccgtacgg gaagctcaac gttgtctcga
    13921 atgtccccat ccgacttgta ttgacgggtg tccggttagc atcaaaattc ccgattttat
    13981 ccggttgatt gttcagcgag attttagcgg agcggcgcgc aaaatcaaag aggataacct
    14041 tctgcccgcg gtctgtggac gcgtctgcct gcaggaggaa caatgcgaaa aaacctgcgt
    14101 cattcgcggc aaattcggtc cggtctcgat tggcagactg gagcgcttcg tcgccgatta
    14161 tgaacggcag aacggtcaac ctcaagtttc tcttagtacg ccgcgcaatg ggaaaaaagt
    14221 cgccattgtt ggttccggac cggctggtct ggcttgtgcc agtgatctca tcaaatgggg
    14281 ctacgacgtc accgtattcg aagctctgca cgaattcggt ggtgttttga tctacggtat
    14341 tcctgaattt cgtctaccga aagccattct caaatacgaa attgacaatc tgcgaaaatt
    14401 gggggtacgc tttgaagcta attttatcgt agggaaaact atgacgatag atgaactttt
    14461 tcaagttgct ggatttgatg cggttttcct gggtgtcggt gcaggtttac cctggtttat
    14521 gaatattccc ggtgagaatt tgatgggagt ctattctgct aatgaatttt taacgcgcgt
    14581 aaatttgatg cgcgcttatg aatttcctga agctgacacc ccgataaaaa taggcaaaca
    14641 ggtggtagtt gtcggcggcg ggaacaccgc tatggatgcc gtccgaaccg ccagacgtct
    14701 cggagctgag aaagccacga ttgtttaccg ccgctcggag gtagaaatgc cagcccgcct
    14761 ggaagagatt aaacatgcca aagaagaagg cgtcattttt cgaactttga ccaatccgat
    14821 tgaatttctg cctgacgaaa atggctgggt aagagccgcc cgctgtctgc aaatggaact
    14881 cggtgaacct gattccgatg ggcgacgacg tcctattccc attccaggta gtgagtttga
    14941 aatcccgact gatctggtca ttatggctat tggaaatggt tcaaatccgc tcatttcccg
    15001 taccacgccc gatttgcaat gcaataaatg gggcaatatc attaccagcg gcgagggcgg
    15061 acgaacttcc aagcgcggcg ttttcgccgg cggcgacatc gttacaggtg gcgcaaccgt
    15121 tattctggct atgggcgccg gccgagccgc cgcgcattca attgacgaat acctcaaaac
    15181 cggaatttgg gttacggagc cggattcagc caataaaaat aaaacttaga ggtgatatgc
    15241 cgatttccaa ttatatagct caatctatga cccgctcatc gtggattcgg aaaatgttcg
    15301 agaccggaat tgctttgaaa aagcagtatg gtgaagataa aatttacgat ttctctctcg
    15361 gcaacccgga tttgcctact ccggagattt ttaatcgcgt tctggtcgaa gaatcagctc
    15421 gtactgggac ttatgtgcat ggctatatgc aaaataaggg ttatcccgag acctgtgcgg
    15481 cggtggcgaa tttgctcgag cgagaaagtg gtttgccgtt taccgaagat caggtcgtta
    15541 tgacctgcgg tgcggctgga gcaattaaca tcattctcaa atctctgctt gatcccggcg
    15601 acgaagtcat cgtaattgcc cctttctttt cggaatatga attttatgta gataatcacc
    15661 gcgggcaatt ggtcatttcc gaggcaaatg cggaactatt acccgatccg gctgacattg
    15721 ccgcgaaaat tacgccgcgc actcgggttc tactgctgaa ttctcccaac aatcccaccg
    15781 gacgtgttta cggcgaagac atttttcgtg agattgggaa attgctgacc gaaaaaagtg
    15841 ccgaatttgg tcgcccgatt tatttattga tggacgagcc ttataagaga atcatttttg
    15901 acggactcca atatcgttct ccatttcgct attatcggca tacaatcctg gcatcctcat
    15961 tttccaaaga cctttcgtta gccggagaac gcatcggtta tctagctatc agcccattaa
    16021 ttgaggatta tccggcttta atcaatgcgg cggcgttctg caatcgggtg ctgggcttta
    16081 ttaacgcgcc agcgttgatg cagagagtta ttcgacgagt actggatgct caagtcgcta
    16141 tcgaattata ccagaaacgg cgtgatatga tttatgacgc tttagtgaaa atcgggtatg
    16201 aaattatcaa accggaaggc actttttacc tctttcctaa atgcccgatt gatgacgatg
    16261 ttaaattcgt tttcgccttg caggatcggc tgataattac gacaccgggg attgctttct
    16321 accgaccggg atattttcgg atttcttatg ctgtacccga agccgtgatt caagccgcaa
    16381 ttcctatatt caaacaagtt tacaatgaaa tgaagtcata attgaacaat ggagcagata
    16441 tgaatgaaac ccaatcagta ccccaaaaaa cggtcacgcg gggacacgtt atcatcaact
    16501 ctgaagaatg taagggttgt gaattatgta tcgcgtcctg tccaccaaag gttttgtatt
    16561 tgactgaccg atttaatact cacggttatc atccttccgc ttataaggga gaaggctgta
    16621 ccggctgtgg aatctgcttc tataattgtc ccgaactggg tgctattaca gttttcaaac
    16681 gctgggattt agtgactgag aaggctttct gtccggagtg cggtagcgaa cagaaagttt
    16741 tttccagaga gggcaatgaa aaggtaaaac tctgcgcggg atgtctgcgc ccggtgtcac
    16801 ctgaaaaggt cataaccatt aaatagcgga ggattaaact atggctaaac aatttttaaa
    16861 aggaaacgaa gccgtggtga aaggagcggt gctagccggt tgtcaagctt attacggcta
    16921 tcccatcacg cccgctagtg aaattgccca cgcggcggct ttatatctac cggtgtcagg
    16981 cggcacattc ctgcaagccg aaagtgaaat tgccgcgatt aatatggttt acggtgcggc
    17041 ggccgctggt cagcgcgtta tgactgcttc ctccggtcct ggcattagcc tgaaacagga
    17101 aggcatttcc tattgtgccg gttcggaatt gccctgtgtc atcgtagatg ttatgcgagg
    17161 gggccccggg ctgggtaata tcgctcctga acagggtgac tataaccagg ttgtcaaagg
    17221 tggcgggcac ggaaactata aatgcatcgt gctagcgccc aactccgttc aggaaatgtg
    17281 tgatttcggc atgctggcgt tcgatctggc tgataaatac cgcaacccgg tttatattct
    17341 ctcagacggt tttgtcggtc aaatgatgga gccggtcgag tttcccgagc cggtaaagca
    17401 gtttcccgaa aaatcctggg cgatcaaagg tactcctgag acttccgata atctaatcac
    17461 ttcaattgaa ctcgatccgg acgatttgga gcgccataat ttgaagctcc agcaaaaata
    17521 tgccattata gaagagcaag aagttcgttt tgaagattat agtctcagcg atgcggaaat
    17581 tgttctggtg ggatttggaa ttatatcgcg cattctgcag actatcgttg acaatttacg
    17641 agatgtggga gtcaaggtcg gtttacttag accgatcact ctttttccct ttcctaaaaa
    17701 gaaaatcagc caacttgccg aacaggccaa attcttctta gtcgttgaaa tgagtaatgg
    17761 ccagttggtt gacgatgtcc gtttagcggt cgccggtcgt aagccggtgt acctttataa
    17821 ccggctgggc ggcaatgtgc cttccgaaaa ggaaattaac gacgaattgc ttaaactgat
    17881 gaggtcggta tgaataacat attgcagaaa tcagccattt tttatgatgt gtttgagcgc
    17941 aagcccggta ccgacaaact taccactact tactgtcccg gttgtggaca tggaaatctg
    18001 cacaaattga ttgccgaagc gctggttgat ttcggcgttc aggatcgcac aatttttatc
    18061 agtccggtcg gttgcagtgt ttttgcctat tactattttc ataccggcaa tattcaggta
    18121 gctcacggac gtgctccagc cgccgctact ggccttaagc gcgctcaccc cgaagccatc
    18181 gtaatttcct atcagggtga tggtgattta gccgcaattg gcaccgccga aataattcac
    18241 gccgctaacc gaggcgaggg cataacggtt tttttcgtca ataatgcgat ttacggcatg
    18301 accggagggc aaatggcgcc aacgacatta atcgggcaaa aaactatgac gacgccttat
    18361 ggaagaaatg ctaataacga gggctatccg attggtatgt cggaaataat cgctactctc
    18421 aaagcccctg tttacgtcga gcgcgttatg attggtgaca ccaaatcaat tctccatgcc
    18481 cgtcaggcgg ttcgtaaagc cctcaaactt cagatcgaga agaatgcctt ttgctttatc
    18541 gaggtgcttt caccctgtcc ctcaaacctc aagatgtcag ccatccgtag tcgcgaatgg
    18601 gtatgcgaag tacttcaaaa acaatttcca ctcggagtga aaaaagacat ttccaatgaa
    18661 tttccgagta gagatttaaa acgtccaaca atcgatcgtt cccagcttca ttctatatta
    18721 aatattgaac cgatcaccgt agataaagaa aagatcgcac cctataaaaa gactaatttt
    18781 tcagcagaaa tcaaagtcgc cggctttggc ggtcagggaa ttttaagtct cgggcttgtc
    18841 ctagctgaaa tgggtatgcg tcatgactac gaagtcagct ggttaccatc ttatggtccg
    18901 gaaatacgtg gtggcaccgc caattgcagt gttaaaatct caaaccgaaa aatcggttcn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ctttctaatt ggtgataata
    19081 atgttttcaa atttttgtaa ttgaattaaa gctggggcat tttttgagat tgccgattct
    19141 acccgtaaat ttaatttgat aatagtacgg tttaatgaaa tttatattta ctacagatag
    19201 aatttttcta cggattttgt tgattgctat tctatccgtt tttattttag taaatagcct
    19261 attggcgcaa atgctcggtt ccgaccaacc gccgccagat attcactggc gtcagataga
    19321 aacacagcat tatcggataa tttttcccgc cgaaatcgaa tcggaaagcc agcggatggc
    19381 taataccctc gagtatgttt acttgccttt acgtaaaaca ctgaaagcga acagtcctaa
    19441 atggccatta gtcctttcca accgcagtgc cgtcgctaac ggttatgtaa cccttgcccc
    19501 gcgtaaaagt gaatggttta cgactcccga tcaatccgct atgtccggca atattgaatg
    19561 gtataatctc ctggctaccc atgaagggcg ccatatggtc cagtttgatc gcttcaattc
    19621 cggatttacg cgcttggctg gatttttgtt cggtgaatat gcgattctgg ggctctcgtt
    19681 ctggcatgcg ccgatgtggt tctgggaagg cgatgccgtc gctatggaaa ctgcattgac
    19741 tgaatctgga cgcggttgcc aaccggaatt cgatctaccg attcggactt tgttaatcaa
    19801 tcaaaaacta tattcttatg ataaagccta tcttggctcg tacaaggatt attatccgaa
    19861 ttattatcat ctgggctttt tgatggtaac tcatgggcga cggcagtatg gcgcaaatat
    19921 ctgggatcac gtattaacca gctcgactcg gcgctcctgg aatccatttg gtttctcgaa
    19981 agcttgccga atcaataccg gtcgcaaaac caaagagctt tattatgata caatgactga
    20041 actcgattca ctctggtgca aaccgatcga agggctggaa atcacctctg ccaaaaaagt
    20101 taacttaaaa ccaaaaaagg tctggacaca ataccgctgg ccgcaatatg cgactgatgg
    20161 ctcaatcatc gccgtaatta atggcttagg tgacatcaat actttagtga agatcaaccc
    20221 tgatgggcag gaaactgaaa tagccaaaat cgccagcgcc aatcggtttt cgactgccgg
    20281 gaatttgatt gcctgggaag aattgacgcc tcatccacgc tggggcgccg cgattttttc
    20341 cgatatagtt gtttatgatt tattagtcgg acaggagcgt aaacttacca agaacggtaa
    20401 atacttttcg ccagcggttg cgccgaatgg gcagatgatc gcggctgtaa aatttacccc
    20461 ggaaagaaaa tgcaacctgg ttttgcttga ctctaatgat ggtcaagaaa tcaggacttt
    20521 atccaatccg gataacggtt ttatcaagaa cccagcctgg tcgcccgatg gaaagcaatt
    20581 agtctatttt aaacaatctg gatcctccaa gtcactggcg atttacgatc tggagaacaa
    20641 tactgaagag attattatcg cggaatgtca ctcgttcgac gaatatccgg ttttttacgg
    20701 cgactatctg ctttataact cagcttggtc gggaattgac aatatttatg cggtagaaat
    20761 tgacagcggt cgtcgttttc aagtgacttc acgcaaatac ggtgcctatt acccggcggt
    20821 ttcagccgac ggctcgatga tgctttttag cgattatgat tttaatggtc tggatgtatg
    20881 cgcaatgcac ctcgagccag cccgttggca ggcgctggaa gcggtcgaac gatgcgatat
    20941 tcgctattat gagacgctgg ttgctcagga acaaggtggt aatattttcg aagcgggaaa
    21001 aatcccaacc agatcgtatc cggtttcgga ttatgatccc tatcggcatt tatttaattt
    21061 tcatagctgg tactatttct tctcagagac ctctgccaat ctcggattat tttccaatga
    21121 tattctcaat aatgccgccg ccaattttga attgaatttc gatgctaacg agaaagcctt
    21181 ttcagtcgga tcggaagttt gctatgccgg tttttatcct atcctgagcg ccggatttaa
    21241 cctcggcaag agaatcagct attccggtgg actttttgac gaattaacca ctgagacttg
    21301 gaacgaaacg tcattctcat tgggtgcaaa cttgccgctg gattattcta gcagtgtatt
    21361 taaccggaag ttgaacctcg gcttgagtgg tgcttatacc tacatcagtg ataaaactac
    21421 cgtcgaagat taccagattg gtaacggtgg ctttgtccct ttttcctatg aaattgccta
    21481 ccagaattac caaaccccag cgattcgcga tgtcaagccg accgccggct ataccgttaa
    21541 agccaatttc aaacatactc cactgggtgg cgattatcaa agtcagatgt attccttcca
    21601 agggaattat tacttgcccg gtttggctaa acatcatagc ctgatgctca atgccaaagt
    21661 ggaactgcaa aatcctgaca attaccgctt tgccagtctg gtgccgtttg ccaaaggcta
    21721 cgactatatt tataacgacc ggttggcggg tgccggcgct acctacgaat tgccgctcgc
    21781 ctatccggac tgggcactgg gttcgctgtt ttacctcaag cgcctacggg gtgccttttt
    21841 cggcgatttc aacgtgggaa aaaccagcga cttcgaacag caatataatt cagtgggtct
    21901 cgacctaaat tttgacttta atcttttaca gctcgcggtc gaattaaatg ccggcgtacg
    21961 gctcgcctac cgtttggcgg aaaaagacct acacgccagc ttgctgattt tcaacatcga
    22021 attctgagcg ggtaactaat ctcttctccg tttatcaaag actttgatga ttaaaactga
    22081 gagttaattt attatgaaac caaacgacaa tataaatcag gttatgatta aaatcggggg
    22141 aatgcactgt gcgatgtgcg tcaaaaccgt cgaagaggct ctcggcaaag tagctggtgt
    22201 ccgcgcggta aacgtcaacc ttgccaatga gaaagcttac ttagaatttg atgatggaca
    22261 tttttcgatc gaagccgcta aaagggcgat tgaatccgtc ggttatcaat atctcggtcg
    22321 ggaaggcgag caagatttaa tcgaacagga taaaaccctg aatcgggaat tgcgctcaag
    22381 gaagcgacgt ataattattg gtttcattac cggcgtaatt ttaatgattc caatgttcat
    22441 caaaattagc ctgccgtttc caattgccta tttgatgctg gcgctagcta ctccggtctt
    22501 catctacctg agttatccga ttttccgtgc ggcagggcag gcgcttcgta accacagttt
    22561 gaatatggat gtaatgtatg taatgggtat cggggtcgct tttctagcca gcctactggg
    22621 aactttcgaa attgttttga cacgcgaatt tatgtttttt gaaacggcta ttttcctggc
    22681 gacatttcta acgctcggtc gctatctgga aacccgcgcc aaaggcaaaa cgacagccgc
    22741 aatcaaacga ttgatcgggc ttcaacccca aaccgtcatc gttattcggg ataatgtcga
    22801 aagcgaaatt ccgctggaat cggttgtaat tgacgacatc gtgattgtca agcccggcgg
    22861 tcgcgtaccc gtggatggag ttgtaattag cggtgaaagc tatattgatg aatctatgtt
    22921 gaccggtgaa tccctgccgg ttttcaagca gaccggcgcc aaggtcgttg gtggaacgct
    22981 gaatcagaac ggcattctac acattcgagc cgaacgaatc gggcgtgata cggttctggc
    23041 gcaaattatt cgtctggttg aacaagctca gggcagtcgc ccgccggttc aacgactggc
    23101 ggatgtggcg gtcagttact tcattcctgt gattttagcc attgccatat tgtcttttgg
    23161 gttctggtat tttgttgctg agcagacatt gcttttcgcg cttacagcgt ttatttcagt
    23221 gctggtaatt gcttgtccct gcgcactcgg actagcgact cccaccgccg taacagtcgg
    23281 catcgggcgt ggcgccgaac ttggtatttt aattaaaaat ggaactgttc tagaagtagc
    23341 accgcgagtc accacagtcg ttttcgataa aactggtact ctgacaaccg gcaaaccgtt
    23401 ggtgactgac atttttcccg tcgggattgc taccgcagaa ttactgcgct tcgcggccgc
    23461 ggtcgagaaa aattccgagc atccgctcag cacggcgatt gtgaattatg ccaaggaact
    23521 gggaattgca attccagtat cggcaaaatt tgcggctttc ggcggtcgcg gcgttacagc
    23581 ttatgttgat aatcgcgtaa ttttcagcgg caccgaatta ttttttcagg aaaataaaat
    23641 cacaatttca ccggatattt tagcgcctgc taagaaatac accggcgagg gaaaatcaat
    23701 agtttttgta gcaattgacg ggcaagcagt cggcgttatc gctattgccg atacattgaa
    23761 agataccgcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgacgggcaa
    23881 gcagtcggcg ttatcgctat tgccgataca ttgaaagata ccgccgcttc cgcaatcgaa
    23941 aaattatgta aagataactt gaaaatcgct atgataactg gcgataatcg tcgcactgcg
    24001 caagccatcg cggcgcaagt tggaatcgaa accgtgctgg cagaagtcct gccgcaggac
    24061 aaagccgccg ttattcgtca gatgcaggaa aaaggcgaag tcgtggcatt cgtcggcgat
    24121 ggtatcaacg atgccgtggc gctggcacaa gccgatatcg ggattgcaat tggttcaggt
    24181 agtgatgtag ccctcgaaag cggcgatatt gttctggtca ggaatgatct aaatgatgtc
    24241 agtgtggctc tgcaactcag tcgcaaagtt atggctcgca ttcgccagaa tcttttctgg
    24301 gcttttgcct ataatgcggc tttagtaccg gttgcggcgg gggcgcttta tccgctgttt
    24361 ggtattactt ttcgaccaga acttggtgga ctagctatgg cacttagttc cgtgaccgtc
    24421 atcagtcttt cactaaacct gcggcgtttt aatccggctc gataaactct ttcatttaat
    24481 tcgctattat tttaatattt agtaatgaaa ataactggtt gcgatagatg gatctcacac
    24541 agataaaaac cattgccgct ggattttata tagcggatga gaacggtaaa aaacttcccg
    24601 gaaccataaa aattccctat gcggaaaaag atggttttat aagacatatc tcccaggtcg
    24661 aaaaaggact ggcttgcggc tgttcctgtc cggtttgcca tcagccggtt atcgcgcgca
    24721 aaggggcaca taaacgacat cactttgccc attaccccga cgcagtttgt aatgccgaaa
    24781 ctgttctgca ttaccttgcc aagcgcctat tgttcgaaaa acttcagact ttattgaaac
    24841 aaaaagtggc tctcgttttg cgctggaaat gccaactttg tagtgatcag cattccttca
    24901 atttctttga aaatgtgcag acggtagtta gtgaagaagc atttgatgac tgccgcccgg
    24961 atctttgtct ggttgaccag cacgatgcac cgacggcttt gatcgaaatc gtcgtggcgc
    25021 atccaccgga cgaaaaagtt ttaattttcg ttagccggaa acaaattccg ctattgattt
    25081 tccgaattcg ctccggtgaa gaaatggaag aaatggctac ttgcccaacg attaatccgg
    25141 atttgatcat ccattgtcca cgtaggcgct gtccggaatg cggtgctccg ctttttgaga
    25201 aatcgctcta cattctgcca gcggtttgtt ggcgttgtgg agctggaatg aatttagctt
    25261 ttcttgatat tgacggacag gttgccggaa cggaggaatt ttcagccaag gatcgccaga
    25321 taatcagccg aaataacgtt atcctgcgag acactatccg tcctggtgac cgtaaacgca
    25381 ccgttagtca tatctgtccc cattgcggag tagtcaccgg caacgcttat ttgcgttacc
    25441 taaaacgtca cgagcaaagg cttctagtcc aaaattccgg caaatattgc cgccaatgtc
    25501 ataggcattt tgacgaatag ttttctatct gagctatttg gcgattattt ttttacgaga
    25561 ttcgagaaac tgaaatccag ttccctatca gaataagaat ccggctctaa aatagatcac
    25621 cgccctgatt tctccaggct ggaaaacact cttgtcgccc caacccagca ctaaatcgaa
    25681 tggtccaagg ggcgagacat acttcagacc gataccgtaa cccaatagat tctgagactc
    25741 ttttatggtt tcgtggtaaa tattgcggaa gccaatcgcc gtattgacaa ttgctttagc
    25801 ttgcaaatta tgcaataggt tgtaggcata atccaaacgc agaatagtca tttcagaacc
    25861 aaccagttga tcatattcga ctcctacaaa agtttcttgc ccgccgcgat aaataaaacg
    25921 gttggtagaa ccgtccatct tagcattccc gtaaaaacct gaaaaatgca aagtgttgcg
    25981 tttgatggac tgatatacgt cggctgaaag ttcaaaacgc cgatagttgg tatgccgacc
    26041 aatttgggag attgtctttt cgtaaacgca atgaaccaaa gcgcctttgc gcggcataaa
    26101 tgcattatcc agaagatcaa tgttagaaga caactgaaat ttatataggt catcattcca
    26161 atcataaaat cgcaacggtt catccggtgc gatatccggt ttgatattaa ttgattcgta
    26221 atccagctcc gcttcgaaat tccagtaatt tttataaagc aaaccgaaac cgccaccgaa
    26281 gagcgctgaa cggtcaatat atgatgcgat tctattgccg ttaatgctat agacataggt
    26341 tggaatttcg cgataatggg cgttgacaaa tggataaaaa ggcagttgga agcgccgcga
    26401 gggataaagc gttttgagct taatctgatt cagtccaatg aactgccatt cgacatcgag
    26461 ccttaatcca tgaactatat ttgaagttgt ctggaaattc agcgccgcta ccaaataatg
    26521 gaagtcatca tatcgcaccc cgaggcgcag tttctgaggt gtatgctcga cgacattcag
    26581 tattagattg atcttacctt ccccggctgg ttccgtagta tagctgatat tattaaaata
    26641 atccagacca tatagataat taatctgcga ctcgatacgt tccatcgaaa aaacctcacc
    26701 agggataatt ccaaaactac gccgaataaa atcatccgtt aaatattcat tgccgcgtac
    26761 ttcgatatta ttgatctgtg ctttgacctt ctcgactaaa ttaattttga tttcaaccgt
    26821 agaatcggag agtatttcga ccgtcggtct gacacgaatg taattatcaa aagatttcaa
    26881 acgctcaaca cccgccgcta gagttaccgg agaatataat tccccaactt taataccaat
    26941 agtcgaacga acgacgtcaa ccggaatggt ttgactacct ttgatgatga gtcgagcaac
    27001 ccggtaacca cttggagaat tcaaatcatt aatattctca ggaccatatc ttgacaagtt
    27061 atattttttc ttcaaatcta ataattcatc gagattagca tgcgctgcgg cttcgcctgc
    27121 ctcaataatt ttgcatacct tagcggtcga aaaatcagcc gctcctaatt tttccaactg
    27181 gcagttgatt agtaaatcgg catttttagc ttcggcttca agcttattat cgcgcaaaat
    27241 attagatgtt tgggacaaga tatccaaggt ggtccgtagt tcatccctgg ttttaaaggg
    27301 gctcctgacg gttgaagcaa ttacgatgtc agcgcccatt tttctgacag tattgaccgg
    27361 cagattattg agcaatccgc catcaaccag aagcgaatcg ccccacacaa ccggggtaaa
    27421 aatactggga atcgccatcg tcgcccgcat cgcaatcggc agagaaccat gatctataac
    27481 cacttccgat ccagtggtca aatcaactgc aacacaccga tatgggataa tcaaactgtc
    27541 aaagtcgctg acggttaaaa atggtagaac taagcgggtc atatagagcg taattttttg
    27601 gccagcgatc acaccccctt tatctaccgg ccgcaaatcg cgaataccta actcaaattg
    27661 atacttatcg gcttcctgct tctgaaaata aggtaaagcc tgtcgggacg gtgcatcatt
    27721 gaacaaatcc gcccaatcaa catttcgagc gatttcttcg atttctgcac cagtataacc
    27781 gacggcgtat aaggcggcga taattcctcc catactggtt ccggcgatat aatcaaccgg
    27841 aatgctcaat gaatcgagca attttagggt gcctatatgt ccaaaaccca tcgcgccgcc
    27901 accgctcaga gccagtccga ttttggggcg agttatcgtt tgagccggta aactggtggt
    27961 agttagagaa agaacaataa tggatataat tccatttatc ataaatcggt gtctattaaa
    28021 attgaggtta gagcaacttg ccatcggaaa attcacatat agtctattgg aaattatcgg
    28081 gctttctcga gcttctttaa ttgcttagtc gctctggcgg aagctttggt atccggataa
    28141 ttgtttataa ttaagtttaa tatttcgatg cttttctcgt tatctttgat tttcgtttga
    28201 taaatatcgt agactttttc gagagccgcg acggcgtttt ccgattgcgg gtatttctga
    28261 aaaatactca tataaatttc aatagcctga tcgtagcgtc ccttggtcat ataaagattg
    28321 cccgcctccg tcagagcatt gaatgcgccg gtcgtggaag gatagcgttc atcaatggat
    28381 ttataggtcc gcacggcttc ttcaattaaa ttcatctggg tagattgaat cttggctttc
    28441 cgcattaaag ccggaatagc gttcggggat tgcggatatt tatcggcata aatctgatat
    28501 tgttcgacgg ctcgttccca atcatttaaa tactcgtcgc tgattttagc gatgcgataa
    28561 tgggcattct cagccagggt atcttgcgga aagcgtttaa ttaaatcagt aaaagtttga
    28621 acggcgtttt tatattcgtt gaactgttcc tgctgaataa ctccgctggt atgcagagcg
    28681 cggcaatata attcatcact tgggtaaagt atcattattt tctgtaatgt cagataggca
    28741 ctgtgcggct ggttgttagc taaaagagct tcggctatcc acaaattggc ttgcgccgcg
    28801 tatttggaat ttggatatag ctccagaaat ttataggctt ccggtatgaa gttgtttcgg
    28861 atagcctgat caggaaagcc tttgagtaat tccacataat gataatagcg ctggtctgcg
    28921 agcggtaatt ggggaatctt attaagcagg tcggttaaaa aagcgctata aggttggtaa
    28981 tatttctctg tccgaaagat gccagcccga acggttttaa tttcctcacc gagcgctgaa
    29041 ttggggaaaa gataaagaaa cttgataata ttgcatccgg cattacccca atctttttcg
    29101 cgaatattga tttggatgat aaaatcgaga actgaatcac tccgagcaat aggaaaatat
    29161 ctatcgtaat attcattcaa ttcttggatt tgaaaaggtg ctatgttttc gatcacggtc
    29221 ggcattttta aaaaaccagt tttatgtctg ggctcttcca gcctgagaat ttttccgtac
    29281 tgaaagtatc gggattcaaa agtgggatcg aggtctttgg tttcgatgac agatttcggt
    29341 ctggcagatc gggctttctc aaggctgtcg attaagattt tattcaaatt attcaggcga
    29401 gtgcattcag tctccagagt tttaatcgtg gtcatataga ttgattccag agagtcaagt
    29461 tcttgctggt acctttctcc atagacaaac ttgagtgaat cagcaataaa ctgccgaagc
    29521 gtgttaacgt cgcctaccgc actttcctca gtcatctgtt gaaaatcata ttccattcgc
    29581 ccactaactt tatcaatcaa ctctttgtcc tgggtcaagc cggattcatt tagaagtaaa
    29641 agacaagcaa atgtcagcag attgataata gatttctgtg ataatttcat tttatcctca
    29701 cgttgatata aatctcaaat ttacttgcac taaattagaa taatttcgtt aaaaaatcca
    29761 atgctgaaaa aatttactac atcaatactt atctctaccc atttaaaaat caaaaaacat
    29821 ttttgctttt cattttagat atgcaacttt atgaagaaat agtggaagag atttatgcca
    29881 tacttaattc ctgaaccgct aaaaaccgga gggacaatcg gcgttatagc gccttccagc
    29941 cccgcgcccc gccaaactac ggcgctcggt ttacagtatc tccaaaatct aggttataaa
    30001 gttaagagcg catcgaatct aaatcgggga aaattcttcc tcgccggttc ggatgccgag
    30061 cgattaaaat atctcgagca atttctctgc gatccggaaa tagatgctct cttttgcgtg
    30121 cgtggcggct acggattaat tcggataatt gatcagctcg attatcagcg tttagcgaaa
    30181 atcaaaccaa aagtattggt tggttattcc gacattacca ttttgcaaat ggctctggcg
    30241 gctaaactcg gttgggtgac gtatagcggg ccaatggttt ctactgattt gggacaaagt
    30301 ctcagttctt ttagcgccga atggctctgg cgtatgatta ccagtaatcc ttatccgatc
    30361 gaactgacta atccattggc tcagccattg caggtgtttc ggtctggttc cgccgaaggg
    30421 gttttgatcg gcggatgtct ctctttaata acgcctctgc tgggaacggc atttatgccg
    30481 gatttaaaag gcgcgattct gatcatcgaa gatgttgacg aaaaaaccta tcggcttgac
    30541 aagcaactgc aaattttacg cttgcacggc gtttttgatc agattgccgg attgattgtt
    30601 gggcagtttg tcaattgttt tccgtcgaat cctaaacgtt cgtttacatt ggaggattat
    30661 cttagtaacg ccttgaaaga tacccatttc ccggtcatta ccaattgtgc ctatggacat
    30721 cttaaacagc gttttacaat tccgattgga atccgagccc gaatcagcac caatcctccg
    30781 cgtctaacac ttctcggttt atgaagcaaa ttgattcagt caaccgaatt gcctatcaaa
    30841 gcaccgccag tctcaagatt tctgggtaac aattcgactt aatttattaa gtttatgagg
    30901 ttgtttttct caataggtaa aaaaggagaa ttgccttgac caaacctgaa gtccaaataa
    30961 atccggagtg gtgcaaatcc tgtgaaattt gcgtcaaatt ttgcccccgc aatgtactcg
    31021 aaatgggtcg gtttacagcc caggttgtcc acccggaaaa ttgcaacggg tgtaagattt
    31081 gcgaaagtct ttgtccggat tttgccatcg tcgtcaccgt ccccgaaaaa gcaaaggagg
    31141 aggcaaaatg aataaatcca atgcaagtag ctcgaattct cgaattttta tgatgcaggg
    31201 caacaccgct tgtgcggaag gcgccatcgc cgctggctgt cgtttttttg ccggatatcc
    31261 gatcacgcct tctaccgaaa ttgcggaggt gctggcgcgc cgtctgccgc aaattggggg
    31321 caattttatc cagatggaag acgaaatcgc cgcgatgggg gctgtgattg gggcttctct
    31381 ggctggtgta aaatctatga ccgctaccag cggcccggga ttttctttaa agcaggaaaa
    31441 tatcgggttt gcaattatgg cggaaattcc gtgtgtcata gtggatgttc agcgcggcgg
    31501 gccaagcacc ggattgccga ctcacgtttc ccagagcgat gtcatgcaag cccgctgggg
    31561 cacgcacggc gaccattcca ttatcgcatt gtgtccctcg actgttcgtg aaacttatga
    31621 tttgacgatc aaagctttca attgtgccga acgctaccga acgccagtaa tgataatgac
    31681 tgatgaaatt gttggacact tgagcgaaaa agtcgaatta ccggatccgg cttcgctcga
    31741 aattgtcaat cgtcaacggc cgaccgttcc gccgtcagat tatctacctt accagatgac
    31801 ggcttcggac attcccccaa tggcgaattt cggagaaggc tatcgctacc atgtcaccgg
    31861 tttggcacat gacgaaaccg gctcaccaac caatgatcca gcccagatcg aacagcagat
    31921 attgcgcatt aaccgtaaaa tcgaacgtta tcgcgatcaa atcgtcgagg ttgaggagtt
    31981 tgagacgcaa gatatggaga tccttgtcat tgcctatgga gcgactgccc gctcggctaa
    32041 aaaagccgtc agcctcgctc gcgaagctgg ccttaaagcc ggtctattaa agctaaaaac
    32101 gctctggcct ttcgctgata tagagatcga agcgctcgcc aaatccgtga ataaaatcat
    32161 tgtaccggaa ctgaatctgg gtcaaattgc tcacgaagtc gaatggacag ttcgccgcgc
    32221 taaaccagtc atccgggtca atcgcatcaa cggcgaacca attacaccag cggaaattct
    32281 ggcggcgatt aaaaattaga gggaagtatg gactatttat attatttgcg cacagaaaat
    32341 atgcctcata tttggtgtga cggttgtggt aacggcattg tgctgaaatc actcctgcgg
    32401 gtaatcaata aactcgcatg ggataagaat gacattgcgt tggtctcagg catcggttgc
    32461 tccagccgca cgcccggtta tgttgatttc aatactttac atacgactca tggtcgggcg
    32521 ttggcttttg ccaccggctt gaaaatcgct aatccaaaat tacacgtaat cgtggtttcc
    32581 ggcgacggcg atgcgactgc gatcggcgga aatcatttta ttcatgccgc ccgtcgcaat
    32641 attgatatca ccatgataat tttcaataat atgatttacg ggatgactgg cgggcaggtt
    32701 tcgccgacga cacctgccgg taaaatggca acaaccgccc cttacggcaa tcccgatcca
    32761 ccttttgata tcagtcgttt ggcggcggcg gctggcgcaa gttatgtggc tcgttcaacg
    32821 gttttccatt acgctcgcct cgataaatac atcgagcaag cccttacccg gaaaggcttt
    32881 gccgtcgtgg aagttttaac gccctgcccg acttcttacg gacgaatgaa taaagaaggc
    32941 aatggcgccg ctatgttgct ttatcaaaaa gaaaataccg tgtcactcgc aaaagccagc
    33001 ggaatgagcc cagaagaatt acggaataaa ctggttgtgg gagttttcgt tgatcgtcaa
    33061 cagcctgact atcaatcgca ttacgaggca gttgtgacta aagcccgatc agaaattaaa
    33121 taaggagcaa aattatgtca tttcattatg aaataaggtt gagtggtgct ggcggtcagg
    33181 ggctgatttt ggtgggaaaa gtcttagccg aagccgccgc gatctatgac ggtttgaatg
    33241 ctactcaaag tcaatcctat ggtccggaag cacgcggtgg agccagccgt agtgaagtta
    33301 tcatctcaga cggcgatatt gattatccga aagccgagaa tattgacctg ttactggcat
    33361 tgacccagga agcctgcgat aaatatattg ccgatctgaa accggatgga attcttatcg
    33421 tagattcgca aatggtaacc aaaattcccg atggcaactt taaagttgtg gctttgccca
    33481 taattcaatc ggccgtcgag cgcctcggta aatttgtggt ttccaatatc atcgctctcg
    33541 gtgtgattta taaattagcg ggtattgttt ccgaagacgc tatcaaaaat gctatccgcg
    33601 cccgcgttcc gaaaggcact gaggaattga atttgaaagc ctttcaaacc ggcatcgagc
    33661 tggctgaagg atggcttaaa gaacatgctc gttaagccat tcaatttgct tcccgaacga
    33721 tgaaatcagc gattcattat tctctgacag accttctcaa tcgggcttcc gatctgggga
    33781 atcatcaccg gaaatttatc cacaatattg agataaattt cgataatttg tttgcattaa
    33841 ttaataacga acacctaact gaccaatttc atctgttacg tttttcgcca caccccgccc
    33901 gcgaaatgaa gctgatcgcc gatttagtag cgaatcgaga aaccttactg gaaatgctgg
    33961 gttgctactt ttctatccaa ttattattat tgaatttcaa ggcaattgac gtcttgaaga
    34021 tgaaactaag tactactact aatcactatg ccgtttatcg gtcgtttctc ttacgcaccg
    34081 gtaacgattt ccgtttgctg accgcggcat atatgaacac cttgctcgat attttcctgc
    34141 cgcgcgctca aagacctgat ttcgtcattt gtggcgttgg tacgcgagtt gaccaggatg
    34201 acattgatct gggaattgtt gatactggac cggcaaatcg agcgatttta accaatgctt
    34261 tcggagcgat gagtaacgaa atgctcaaat atgcctgttc acttcacttt catctttccg
    34321 agcatgttgg caccaaaggc tatacggctt caattgagga ttaccacaat ctactcgacc
    34381 accaaattca ggattttgtg ctgattagtg aaatggtcaa tgctgttcca attctcggta
    34441 atcgtgaatt atttgaccga tttaagaatg aaattctcga tcgctattat ttttcggctg
    34501 accgagataa taaataccac gaaggttttt tgcgcggctt attgggtgaa attcgggact
    34561 tgatgtataa agagccgccg gaaaatttac tcaatcccaa acgcgacggc ttgcggatgt
    34621 tgaaagctct aatttttgca ctgaagactt ggaagcgggt tgagggcaac acctcgctgg
    34681 aagttttgga cattttatta gtcaaagaac ggttcaatca tgaaaattat tatcaggttt
    34741 ttcaggcact aacttttctg gaaactttcc gctttctgta tcaattgttc gtcgtacagg
    34801 aggaagaaat tcatctaaac agcgatgaaa tgcaacttag cctgcaaaaa gtagcctatg
    34861 cgatgggtta cgaagatcat agttacgctt tagccggtac tcaaatgttg actcactatt
    34921 atgatcatca taaaatcgcc cggttgggaa ctgagcattt actggaaggg ctgaccgaac
    34981 atcttcgcca gattacgatc ttctacccga tcacgcattt ccaatcccgc gccgaaagca
    35041 aagcgcctct gccggataat attccactta attttatgca gactgcccgc ttttttcaag
    35101 gtgtacgttt ctgggatgat ttactctcca gtctcgataa tcccaaccaa attttgctga
    35161 aacgctttct ggcagatttt atgaaattat cggaagcaag acgggctgtc ctgatgaaag
    35221 cttatgtcaa gtggggtgta aaaactcctt atactatgat ttcgctgttc agcattatcg
    35281 ctgaccatca accgaaaata gtcgaaacgc agtttttcca ggaattcatt cgcgatttta
    35341 tttataatct ggaaccgact ctggaaaatg tcagccgact ttgtcaaatt ctgaatttag
    35401 ctccgcagat gatgaataac ttcctgtcaa ttttaccggt cgagctcttg acgcggttga
    35461 tagcagttgt cgaagaacct tactggaaac cagaaattac ctctctgcgc gatacactat
    35521 taatgctttg tcggatttat cgcagtagta gttattattt caagcgtttt gtacagcggg
    35581 cgttcaatac ttatgctgaa tacatcactt cgttaaccaa tcaacctaag ctcaatcaat
    35641 tggctgaagg tcttttccgc aatattgata atttcgattc gctcaatgaa aaaattaccc
    35701 ggttgggtga ttattacgat tttgagttta tgcgtttggg aattgcttta ttagacggcg
    35761 cgcccttcga taaaattaat cgggaattca cgaatttttc ggataattat cttcagatgc
    35821 tttttgaact ctgtcaggag gaagtagcgg cggctgatca gaaagagatc ccggaaatgc
    35881 atgatctttt ggctgttttc acggctggcg ggcatgcccg cggtcaggct ttcgatgatg
    35941 actatgactt aattatcttg ttgaattccg atgatccggc tatcattgac ttcagtaata
    36001 aagtcataca aaaaatgaat aaatacatcc tgcgacgttc catcattccc caattccgct
    36061 ttgctgaccg tttccggcga tttgtgacca ctttcagcga tctgcgtaga ttattcgagc
    36121 gacccgatga aaatactttc attgatcaat cgcaattgct gggtgcgcgt ctggtaattg
    36181 gaagtgatta ttttttgaaa gtatttcaga gtgaaattat tactccattc attttccagc
    36241 gtaaggctga atttatcgaa gcgatgttga atgaaatgag atcccgccac aattattata
    36301 atagtactaa aattgaagtt ccggaaatca aggaaggtcc aggcggcttg cgcgatttcg
    36361 agaattttat ctttattcta aaagcctatt tcgaatgggc tgaacctttg accgaaacat
    36421 tgttttcgcg gattgaatcg gcaataacca atgacgacag taacttcata tctctggtgc
    36481 gcgattatca gttgctgaaa catattcgcg atctatatcg cctgtcagtt tctaacgatg
    36541 accacctgga taaggcttac ttggatcagc tggttcgacc gttttgcctt gcccatggtc
    36601 tggaattaga aaatggcgcc gaattggcgg agttcatcca aaggattatg cgaaaaaacg
    36661 tagagagaat caacctttta ctgcggacat tagaattgcc ggaaattggt gcattgccag
    36721 cgaaatgatt cgccaccttt aaaaatttga gtttgaaact atttggtagt tcatagtatc
    36781 ttttttagaa cagttaacgc tgattcaatt gatcgccaga tttttgagtt tggttttcaa
    36841 tttcaggcat ggttttctac tttagaaaaa tatttgatga ttgatatttt ccttgtcgca
    36901 aaagt
//
LOCUS       DAOMJU010000011        35836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_988_length_35836_cov_20.901428, whole genome shotgun sequence.
ACCESSION   DAOMJU010000011 DAOMJU010000000
VERSION     DAOMJU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 35836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35836
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_988_length_35836_cov_20.901428"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLO58_02040"
     CDS             <1..271
                     /locus_tag="PLO58_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQO73601.1"
                     /translation="RKQAEEQREKFIAELQEALDKIKTLKGLIPICACCKKIRDDQGY
                     WNSVESYIKDHADVEFTHGICPDCMKKLYPNYCKDDNDKKKPETK"
     gene            complement(273..>3016)
                     /locus_tag="PLO58_02045"
     CDS             complement(273..>3016)
                     /locus_tag="PLO58_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HQO73602.1"
                     /translation="VKEPIPNFAEEFFLTKIAEHPDYLENYLLLAECYALNDKAVESE
                     LILREALRRAPTNAMIYLHLIEAYLRGEKYDEAKSAYEKIYQLDPDIPGVLEYRISEL
                     LAKKNYDEAKNLLDRLAQLIPDSPNLQLQLIAYYSKRGLTEKVIEITNAAYHQFPDNW
                     NIVETKANLEAYVKQNNKAAIPILRKYVKNHTEQTAFLKLADYYKKAADLKNWEKTYR
                     QAIEYYPASPSLFYQMAQNFYNRHNYSTALDYIEKALQICPQCAGYWGTRAEIQRSSG
                     QSAAAIKSYQRALELSPTYYDARDKLRQLQGRASIFNNFDTVDIDSLFRNCPSSEQYP
                     GEKAVILYNACRRVVYGGATEMSQEILATVFNLEGIDAFKEYRISYNAYNEELIVEKA
                     QTLKKDGSKIDADVSWGYIVFKSLEPGDAVYLKWKIRNYYSGKLSQHFWDDFSFNTFF
                     PVREVCYALLIPPNVKFQYKGQFMPDEPSAIRETPDGILYEWCLNDVPAIKEEYNMPP
                     LADIGKILHISSIPDWDYIVNWYIDLAETRTRSCYEIDEFLKTLLPENEPLTEQEKIT
                     RIYNYITENIRYSSVSFRQSGLIPQKARDVFVTRIGDCKDKAALGIAMLRSAGFTANF
                     VLVNARYEGLNKHVLPSIAFNHAIVSVATQEGTLYLDLTAQNFPVGSVPLADQGAFAL
                     EIKKGCQPFYLTRHFFMGSVRQRRTKVEVQTDNSIQVELHTTRKGAQTAEFRAVYRFK
                     SQADRDKELIESLAQDFPNVELISFNLFDIDSIKPTLDYEYTFKVPDYLTEAGKFLIL
                     RIPWADRLSPIRALSYDKRNYPFTYWPNADTISEEITITFPAGYQPLEFTPYYEYSCP
                     IADYRLTLDYNDRILKGARLMINKESEITPEIYPAFKEFYNKVVKADGYQILIKRLE"
     assembly_gap    3017..3116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3117..3844)
                     /locus_tag="PLO58_02050"
     CDS             complement(<3117..3844)
                     /locus_tag="PLO58_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73603.1"
                     /translation="MRATRFFRNTILIILLFGESLFSNIFTSAQYVEEAWQAWNRNDQ
                     KLVEQKFQEAIQANPNDTRAYIGLAYLYEMQYKHLEAWQTYAKVIDTEADFEPYIFAA
                     MMAPRLTNAEEYKSAGIQMLFDNLARTARHSTMRINANEKLGHYYNEHANLKKALFYF
                     DKIDAITDWMLIGPFDNISASGYDKVYPPETEFDTAAVYHGQNDIAIKWFRIPQVRYD
                     KWIDMRRYFADNDAVFYGNTFIFSP"
     gene            complement(3992..4468)
                     /locus_tag="PLO58_02055"
     CDS             complement(3992..4468)
                     /locus_tag="PLO58_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73604.1"
                     /translation="MLKQFKVLLIWGILIVGFMACNDNGSEPPTAATLSLLWTEYGGC
                     NEYKGGDILPKAAIQNDSLSWEISDDTLKIFTGITYICCAPFDVESEQSGDSLKIMIR
                     DTCGEIPHEKCYCKCICYYTFLTIFTGYSGDRYHLAVWLHSPRQAQDSLLWQVEIP"
     gene            complement(4533..4976)
                     /locus_tag="PLO58_02060"
     CDS             complement(4533..4976)
                     /locus_tag="PLO58_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73605.1"
                     /translation="MALNFLERFPLLYDWLKAHTTLLKYAGLISLITFFGTILLMGVL
                     ILMIPTDYFSNPKRRKSFLITKRPLLGVILLIVKNLIGLIFLIVGFLLLFIPGQGILT
                     IMIGIILMNFPGKYRLERKLVQRPGVLQGINKIRKKFNRPLLGFE"
     gene            complement(5117..5731)
                     /locus_tag="PLO58_02065"
     CDS             complement(5117..5731)
                     /locus_tag="PLO58_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015106973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HQO73606.1"
                     /translation="MKIIKKYSHLNGEEYLIVHHKDLYNEIKSVIKNINAEGLKTKVS
                     KENRKAGTQLYSPIELNKAFDREFSKRNWNESRYNYYITLNRELMEKSLLMPAGEQKK
                     FLIEHGEKNPIFSYNQTDFVKNKVAVEVQFGKYAFVAYDLFVKHMLFYSGGMINLGIE
                     ILPTKNMQSQMSSGIAYYEGEVYNVMRQGRNSPPVPLLILGIEP"
     gene            complement(5728..6681)
                     /locus_tag="PLO58_02070"
     CDS             complement(5728..6681)
                     /locus_tag="PLO58_02070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HQO73607.1"
                     /translation="MEVKETLFNYVNPAIEYQILNGDSCYILQNLPDGKFDLIITSPP
                     YNIGKEYETKTCIEKYLETQEEIIRQLIRVLSNQGSLCWQVGNFVDKGEIFPLDIYYY
                     PIFKKYQLKLRNRIIWHFEHGLHASKRFSGRYETILWFTKTDNYIFNLDAVRIPAKYP
                     GKLHFKGPNKGKPSGNPLGKNPSDIWKIVVEDWDNEIWNVPNVKSNHPEKTEHPCQFP
                     VELVERCVLALTNKSSWVLDPFAGVGSTIIASIKNERNCIGIEKEAHYCKIAQNRIEA
                     LKAGNLKVRPINKPIHKPSKNDKVANVPNEWLKLKFNGYSK"
     gene            complement(6789..7124)
                     /locus_tag="PLO58_02075"
     CDS             complement(6789..7124)
                     /locus_tag="PLO58_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HQO73608.1"
                     /translation="MNTEQLFESLRKLQEPKGYFFNKDRQHVDRLLNSLLINKERYGY
                     MACPCRLAVGDRESDRDIICPCVYREPDVKEYGRCYCGLYVSADWNAGKIPDRYVPER
                     RPIEKILGL"
     gene            complement(7121..7372)
                     /locus_tag="PLO58_02080"
     CDS             complement(7121..7372)
                     /locus_tag="PLO58_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQO73609.1"
                     /translation="MDKKVKLYALSTCIYCQNTKAFLKSLGIEYDSIDVDLLDKEQKL
                     AVIREIHKLTPTVAFPVIVIGDNVIVGYRPEEIKKALGL"
     gene            complement(7438..>8959)
                     /locus_tag="PLO58_02085"
     CDS             complement(7438..>8959)
                     /locus_tag="PLO58_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQO73610.1"
                     /translation="CFRRNIIGIDYQYNFPGGRRPILYADWAASGRLYRPIEEYITNV
                     LGPYVANTHSDATLTGTIITDAYHQAHKIIKQHVNAGPDDVLLLAGFGMTAVINKFQR
                     LLGLRVPDKFKEKISIPPQERALVLLTHMEHHSNQTTWEECCCDTSLICKKSDGLPNL
                     EHLRNILELNRDRKLIIGSFTACSNVTGLITPVNELAEIMHQFGRLCFIDYSASAPYV
                     EINMHPPKPEQKLDAIFFSPHKFLGGPGSSGVIVFDKKLYNNRVPDQPGGGTVLWTNP
                     WGEHRFFQDIEVREDGGTPGFLQAIRAALAILLKEEMGVENILAQEHYLTEILLNELA
                     GIPDINILESQNHNRIGFVSFYSLKVHHNLLVRLLNDRFGIQTRGGCLCAGTYGHVLL
                     HIDDQRSKAIMSQIDQGNLSLKPGWVRISLHPTMTEVEVRYIASAVREIMKNYVEWGK
                     DYHLNPETGDFDHLKQPKASIKLRETFKAALRIFLPVVILNAYICFSFGNQIAISL"
     assembly_gap    8960..9059
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9253..11265
                     /gene="uvrB"
                     /locus_tag="PLO58_02090"
     CDS             9253..11265
                     /gene="uvrB"
                     /locus_tag="PLO58_02090"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQO73611.1"
                     /translation="MNEFKLLTNFKPTGDQPQAIRELTEGILRGDKFQTLLGVTGSGK
                     TFTMAQVIQNVQKPTLVISHNKTLAAQLYGEFKAFFPENAVEYFISFYDYYQPEAYLP
                     VTDTYIEKDSSINDEIDKLRLKATSSLISRRDVIVVASVSCIYGIGSPEEYTRFIVTL
                     KKGDRIGIRQLFGRLVDIYYARNDLVLERGKFRARGDIVEIRPAYEDYAYRIELFGDE
                     IEHIYICDPLTGEVTNEQEMVVIFPAKHFISSEETIQRAIVSIEEELEARLEELRSAG
                     KLLEAQRLGQRTRFDLEMIQEVGYCSGIENYSRHFSGRKPGQQPYTLLDFFPRDFLLI
                     IDESHVTLPQLRAMYNGDRSRKEVLVEYGFRLPSALDNRPLKFHEFEEMVNQAVFVSA
                     TPSDYELEKCNGVVVEQIIRPTGLLDPEIIVKPTKHQIDDLIDEIAAVTARKERVLVT
                     TLTKRMAEDLSEYLRGMGLRVRYLHSEIKALDRVSILRDLRLGNFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSEKSLMQVSGRAARNINGRVIFYADEITESMRKVIEEV
                     SRRRKVQQDYNIAHNIQPHTIYKSVAEVMQTTAVADVKPTPIQTDQPVGDRYRAKMET
                     SEIIELLRREMLAAAENLEFEKAAELRDEIARLTGQKKSLSRENLL"
     gene            11436..12731
                     /gene="purD"
                     /locus_tag="PLO58_02095"
     CDS             11436..12731
                     /gene="purD"
                     /locus_tag="PLO58_02095"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQO73612.1"
                     /translation="MKVLIVGSGGREHALAWKLSQSPYIAKIYCAPGNAGTALLAENI
                     PLKDNDLDGLLKFALENSIDLTVVGPEIPLVNGIVDLFRAQGLKIFGPTSAAARLEGS
                     KVFAKQFMSRHQIPTAAYQVCDSRAAMESATHFKTFPYVIKVDGLAAGKGALIIHNDD
                     DLREAFEAIWDEKRFGTAGNKVVVEDFLLGEELSVFVITDGEHYLILPPAQDHKRIGE
                     NDTGPNTGGMGAYAPAPLGTPQVMHNVEKQIIIPTLNGLKAEGIPYTGVLYCGLMING
                     TQPYVVEFNARFGDPETQVILPLIESDLGVILHSAVEGKLDATMMKLSRKTAVCVVMA
                     SAGYPGDYEKGKVIYGLQDEKPESDTIVFQAGTIRNNAHFLTNGGRVLAVTALADNLE
                     KAIARAYERVAEITFVGAYYRRDIGQKGLLRQKDLLEKI"
     gene            12747..13970
                     /locus_tag="PLO58_02100"
     CDS             12747..13970
                     /locus_tag="PLO58_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQO73613.1"
                     /translation="MDNERINKLQQSFGILGESDYIKEIVETIDQVARTDITVLICGE
                     SGTGKEVVANAIYRHSLRKHQPFVKVNCGAIPAGILESELFGHVKGSFTGAIEDRKGY
                     FETANHGTIFLDEIGEMPPETQVKLLRVLELGEFLPVGGSEVRRVDIRVIAATNKELA
                     TEIERGNFRKDLYYRIKTITIYMPPLRKHPEDIPLLVEKFALDFANRNSIAFKGFSPT
                     ALNLLREYSWPGNIRELKNFVESMIVLEKGEVITSSMVEKHLLGEPRHIPSPHLPMKI
                     SKSVDQAERELILQQIMVLRQELHEIYEAVITNPLPLKYEALRELEIPPSLPRPKVEE
                     KEIGKLDNAEIINDEIINPEMVGKVTLAEVEKELIQRTLKKFNYRKRQAANALQISER
                     TLYRKINEYGLEESR"
     gene            13976..14503
                     /locus_tag="PLO58_02105"
     CDS             13976..14503
                     /locus_tag="PLO58_02105"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HQO73614.1"
                     /translation="MLFKRLAPIIKFIPAVLLCLIINSCWLYSFKGTLPPDIKSIAIP
                     LFEDRTAEFNIQQIVTDKIRLGFIKENLLKVVEEDNAHSVLYGTILSIDDKPLVYDED
                     ENVGESVTEYRVTIKIEIEWYDKVNDRTVFKKQFIGYSEYDPTGATSRTREVALSEAI
                     NQITEDIMNTILADW"
     gene            14564..>14700
                     /locus_tag="PLO58_02110"
     CDS             14564..>14700
                     /locus_tag="PLO58_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQO73615.1"
                     /translation="MQSVFIKDIQNYNNQEVTLKGWVYNLRSSGKIWFLLLRDGTGLI
                     QG"
     assembly_gap    14701..14800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14801..16014
                     /gene="asnS"
                     /locus_tag="PLO58_02115"
     CDS             <14801..16014
                     /gene="asnS"
                     /locus_tag="PLO58_02115"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HQO73616.1"
                     /translation="SGKIWFLLLRDGTGLIQGIVSRNDVSEKIFNYKEILTQESSVSV
                     SGLIHEDKRAPGGFEIIVKDIQVYQIAKDYPITLKEHGIEFLMDNRHLWLRSRKQWAI
                     LRLRHAVYYGITQYLNENGFIRFDSPILTPNACEGTTTLFEVPYFDLGKAYLSQSGQL
                     YLETGIMSLGRVYDFGPVFRAEKSKTRKHLTEFWMMDAEAAFVEHEENMRIQEELIRF
                     VIRKILAECQPELEILERNIEQLRKADAPFKKMTHREAVEYLRSKGSSMNETEDLGAQ
                     DETLLTEGSDVPIFVEKWPKEIKAFYMKRDSENPDLVLGDDLIAPEGYGEIIGGSQRE
                     DDYDLLLQRMIAENMPLDEYQWFLDLRKYGSVPHSGFGVGLERLITWIAGVPHIRETI
                     PFPRTIYRLRP"
     gene            16014..16469
                     /locus_tag="PLO58_02120"
     CDS             16014..16469
                     /locus_tag="PLO58_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HQO73617.1"
                     /translation="MTVSPKIIAVLGGRECDEQTYQLAFDVGRAIAERGAILLCGGRT
                     GVMEAACKGAVSAGGLTIGILPGADKNDANPWVKIALPTGIGVARNAIIARACDAAIA
                     IGGKYGTLSEIAYCLQLNKPICGLNSWPEISDLRQFNSASAAVEFAFGE"
     gene            16474..16749
                     /locus_tag="PLO58_02125"
     CDS             16474..16749
                     /locus_tag="PLO58_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQO73618.1"
                     /translation="MAIRVAIIVQGVVQGVGFRWYVRRIAMRYRLTGYVKNLPDGDVY
                     CEVEGEEGMINDFISELKAGPPLSHVTNVIVNKLPDLVGYKSFEIRF"
     gene            16746..18404
                     /locus_tag="PLO58_02130"
     CDS             16746..18404
                     /locus_tag="PLO58_02130"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HQO73619.1"
                     /translation="MKQIIKIGLAQINPVLGDLPGNAKKITNFIKEADQQGIDLLIFP
                     ELAITGYLPQDLLLNRSFIDCNLQCLNKITAACRSSMVVILGYVARENGKLYNAAAVI
                     KSGQLIATCYKTLLPNYDVFDEHRYFTPANENLPVPIEIGGHSVRLGVEICEDLWDSN
                     SAIKVTDQLVAQGAELLVNLSASPFEYDKRDRRRQLVLDKVTKLRRPFVLVNLVGGRD
                     ELVFDGNSFALDASGALIGWGGEFKENLAVFELDLVSGQGHRMPWPETQREASIYQAL
                     TLGVHDYFYKTGGRQAIIGLSGGIDSALVACIAVSALGAENVLGVFLPSQYTSAASRE
                     DSVVLAKNLNIRLIEIPIEKIFQSYLQELKPVFGQTSPDVTEENIQSRIRGNLLMALA
                     NKYQAYVLNTGNKTELALGYCTMYGDMCGALAVISDLSKLDVYKIAGYFNETAERLII
                     PERIFTKIPTAELAPGQVDPFDYTIVSPLVDLIINDQKTPAELIQMGYAADLVNDIFE
                     RIKFAEFKRRQAAPGLKITSKAFGLGRRYPIINHFKEKGGKQCE"
     gene            18395..18859
                     /locus_tag="PLO58_02135"
     CDS             18395..18859
                     /locus_tag="PLO58_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73620.1"
                     /translation="MRVVRYWLTLICIGIALYTASPLLAQNPTDIDGTIWRRMKYDNK
                     YYWLLGYLQGLEKSDEIIQLCLAEQEKREFAFTVPFYVDQMRARISAYFPPHNIQDIT
                     QLIELLNTFYKDKYNQKIKIEAALRIVLARQKGQIEQSDFWLNEARRALIQK"
     gene            18885..19421
                     /locus_tag="PLO58_02140"
     CDS             18885..19421
                     /locus_tag="PLO58_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73621.1"
                     /translation="MNDHTEFEEINLARHIYRYLITIGIALFGLFLISIEPNVLRQYR
                     SWIPSQTFNIYYLTWFAVCTISAILCFFWGVLCQFLRGNNCLGNVLWYVGIFTITLFF
                     ATVFFQILKTDRSVGLAVTFGTLIISAMVTMAISYIFYLLNFNSRYHFWLFTLAIISA
                     TSAYNWLYLSVIGKIPAF"
     gene            19456..21819
                     /locus_tag="PLO58_02145"
     CDS             19456..21819
                     /locus_tag="PLO58_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HQO73622.1"
                     /translation="MIKYILERMPLVGICVVYLSGIVTDHTLHLLPEVWFIFLLIDFL
                     IGIIFSSKARSVLLIAITLFCLGGMNHALRTVTGPDHLSQFYFSNEPDSVIAMVEQAE
                     FSGDGKLKIRLKNLQIKHSDWQKWNGKLILTVKEPENNVIYGDWMAFYTRLNQPATAR
                     NPGEFDYRRYLANHHIYATAYLDAGQTPTIIGRSRSLRRAANYSRIKVEELIDKSMSG
                     EQNAILKALIVGVRGEISDETNQAFVDTGVVHVLSVSGMHVVYVTMVIMLILGFLRIP
                     KKPKMVLAILGLGFYALMVDLRPSVNRAVIMAILTLIAQGWEKRANVYNTIAAAALIQ
                     SLFDPLQIFDMGFQLSFMAVISIVYVYRQLEYLLPLPLKTSNIKNRRLRETWQLFLVS
                     LSALLGTIPITVFYFQRIPLISLVSNLAVVPLVGLIGGLGFGQVILGFLWSGFNLTFG
                     EVQTLLISLLTQIVKISGRVPIAYLTIPAISLAGVYFGYLILLILLNLDKKRVQKVAL
                     FGFLIGINFLIWTQNLSKPVLQVTFLDVGQGDAIFLEFPNHETMLIDTGDRTFRRDYG
                     ELTIAPFLKRKGFQRIDYLALSHPHNDHIGGAPYLMRNFQIGEIWEPDISASSQIYRE
                     IHTLADSLQIPIRHLYAGDCFPLAESLTVFVLHPSKAFLESHPPGFNDYSNVLKISYR
                     QIDFLFCGDVEKFSESYLNLWRATLQSEILKVPHHGSSTSSSQTFLEQITPDLAVISV
                     GKNNKFRHPSAGTIARYEALSTEIHRTDLSRALIINSDGHKYWIKPW"
     gene            21914..23128
                     /locus_tag="PLO58_02150"
     CDS             21914..23128
                     /locus_tag="PLO58_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQO73623.1"
                     /translation="MVNNQPENPEILKGYTAHWIVPVSQPPIEKGVIIVQGQQIIEVE
                     SADNLQTNIETPLIDLGDSVIFPGFINAHTHLEHPPLAETPVNFRKYMQFVCDFTRTD
                     EISKADYAAANLAENINFGTVALADFTSKGASYSALMENPVFARQFFEVTGFKNYQAS
                     EIIRTFRELIQDQTPVKKITKHLTPSYVWATSPQLLHEIAFTERHIAIHIDMIPEENE
                     FTLNGSGLLRQILLANEDFDYSWQVPGLTAIRYFFANHFYARHNILTHMNCVTGDEID
                     FIKEFGVKVNVCLCPRSSKILSQIKTPVDLLLEKGINLCLGTESRVLVPDFDIRKEMI
                     ECVDAYGVSPELALKFATLNGAYAIGFHKEVGSLEKGKIARCLVVDCSGLTTTDPYEM
                     ILNTPHSPRWLI"
     assembly_gap    23209..23308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23510..24652
                     /locus_tag="PLO58_02155"
     CDS             23510..24652
                     /locus_tag="PLO58_02155"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73624.1"
                     /translation="MIREQILKDLIHLPQNKFPVTSAYFTLDNIAGNRKSHIVEIKKQ
                     IRYKKEKTYFRQLSIEEQESVLSDFDRILHWFGKELNPAESISSICFSSSGANLWRTL
                     NFKKPLPQNILVIQPYPYIRPYTMLFSRYRNYAVVLIDRSKAKIFESHLGEFREHFHI
                     EDNAPESVKVGGFKGRQERKVERNIHQGVIQHYKEVAQEIFDLHQKFDFHWIILGGRK
                     EVLAEFSKYLHPYISARIAGQIEVEPTAPLAEVLKKVEKTEEQARNNYEQALLKSLLD
                     RKSAGQTVDGIQAVLDKIQDNWVDKLIIQEHFFKKGLFCRNCGFLSLKPLASCPTGCG
                     KLERTNDLVEHLVHRALKQGVDLQFVSSNMDDYAGIAAILRFSIGG"
     gene            24873..25820
                     /locus_tag="PLO58_02160"
     CDS             24873..25820
                     /locus_tag="PLO58_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73625.1"
                     /translation="MKAKNFYVSIILAISLSACGTSRAKPEKTGEETTKAKSEKAENL
                     PESQSSKSTSSGPQPKIATAPVKATAADQSRLINELTFEVKKLSAEVKHLQSALKELQ
                     AKSQMWDNPLSIYDKEIIMANGTSIFGKIIYQDEKMIKVETWIGYLIIDKNQVVRVVT
                     NIVEKPDEQYVPPELASDIKDDKPITIQAPYISKTGEPAPIGEPTSRVPNCVLVGNIK
                     ERKDLSGNTILSGEIKNIGGRRADFVKINIVFRKNWSGDTETRTAFVEGSYVTFEGTG
                     ITSNNSLLPGATGTFEMIIPKDFGQFIGYTYNIDWEVYR"
     gene            25820..26599
                     /locus_tag="PLO58_02165"
     CDS             25820..26599
                     /locus_tag="PLO58_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73626.1"
                     /translation="MPRSFNLSLLTVVITAVICFGQGTSSSSGTPIHDSNKTIDQKDI
                     EIYNLKEELEYLKKELRKLQEINTSLCDSLESKHTLLETTSEKNVDENYNLRIYHQTI
                     EDDRRLNQEIVKEIQSTAPIAVPFKSPEPSGDPEFRERYNEALNQYFDGNLSDSQKNF
                     KKLLELRQDHPLSDNCQYWLGECLYSQEKYEEAIAAFQTVKALGDGNKADAALFKIGL
                     CYLKLDKKAAASSAFNDLINFYPKSDLVPKAYQYLLSQDKF"
     gene            26601..27326
                     /locus_tag="PLO58_02170"
     CDS             26601..27326
                     /locus_tag="PLO58_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73627.1"
                     /translation="MKKFLIMITLPTLLSAQAFDSSRAVGTPNIMYDLGIFIEPARES
                     APLPKGVANPNLVPIQNQIDSLLSAFSIMNNQIRSLEQANDSLQKKVNELSKRIFLSP
                     FASLTVPAQIYSRHEGERFLQRGRAAYQANNYPVAIDYFLKVLASNMPDATIGDTYFW
                     IGYCYVQMQDEYLAIEYLKKVAEYPLSEKLDDALFLTGITYQKLGNPQMAEIFFSRLI
                     KRYPEGNLARLAALELKRLKGPD"
     gene            27349..28692
                     /locus_tag="PLO58_02175"
     CDS             27349..28692
                     /locus_tag="PLO58_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73628.1"
                     /translation="MTRPHLKISLLLLLTITFLQGESVTRLSFSHPGYMMRIPVSSVY
                     RTPYVFRTGFASDTYGFSDTLIAKGIFFEADLTNTFKVGLTTIQGISGTRPVEFGLHF
                     QKRVFTYGDISFTAGVHDMVLRQGEKNLNIDTETLSIFGLLSNEKQFTNNILHTYMGF
                     GTGGLASTFGTDSTANMGVFAGILVHTNIRKDQGGVSFMVEFDGGGVNLGTRIPLTQD
                     YNLSIGINDVTHLYNFGSKNYSTTPQNEHPSLSISLDFTIPRIKPENIKRRALEGMVD
                     PHTKAILDVEEQFAAKLDSTLKAADFEIAKLRDSLRVYETQIKYLTSQVTQLKQKSAV
                     LEDSVRSIKLAKYAMERNINLALKHLSNSLRYFYEGDYQQALKEVDAAIELNPNLALA
                     YARRGSIYYKMGDIERASINWNLALKIDPEYDDVRNILRAMSENRLRSVGEEQKE"
     gene            28701..29495
                     /locus_tag="PLO58_02180"
     CDS             28701..29495
                     /locus_tag="PLO58_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQO73629.1"
                     /translation="MDIATIFGIIAGTVLIIMGISGLTSDFKIFWDISSVFIVVGGSF
                     ASTMISFSVKEVFSLIGSIIAVFKKEKIKMTDEVETIVELATVARKSVQDLEKALVDV
                     QNPFLRDGLQMVVDGYNPQEIRDILETRIEYRTLRERGEANVLRTMGKYSPAFGMIGT
                     LIGLVVMLYGMAQISSAGLDPMKVLGEGMGAAIITTFYGVILANLIFNPMAVKFETRI
                     QKQNILQMMLIEGILLLQARKHPLIIREKLNSYLRPRDWKNPEEIK"
     gene            29498..30187
                     /locus_tag="PLO58_02185"
     CDS             29498..30187
                     /locus_tag="PLO58_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQO73630.1"
                     /translation="MADEQNPRHKIFSHQDTGDEEPWLITYSDMMSLLLTFFILMFSI
                     STIDPVKLEQIGNSVGQALGGAKPKPTEHFSIKEIYDKVVDVIEQEQLKGVIEVETSS
                     RGVSIKIPSEISFASGKADLDPRIFPILNKLAPMIAQSKFPIAIEGHTDNVPIKSDVF
                     PSNWELSSARAAQVVRYFVGLGLPSDRFRAIGYADTRPRASGKTIEEANQTEEQRAQN
                     RRVEIIFLTIG"
     gene            30203..>31452
                     /locus_tag="PLO58_02190"
     CDS             30203..>31452
                     /locus_tag="PLO58_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HQO73631.1"
                     /translation="MSHFKVKQLILIFSLGLFTLLIAQENLSYLSQKMSLENSIKDRI
                     TEALEKLLDDVKFVVNVKAEIDFAPIEQAEPTETTETPPVGTTPQESRPRATEYVLPL
                     PGFDIPTSVIQSQERIQSQTQLDALPQPATGAAKSTSTTEKKVATSVPVIKRQEINVI
                     LEDGISPEIIENVRQVVAVAAHYDRARGDVISIMTANFKKSPDKDATETIILKNIAEK
                     IDDIERRQTAAEYNARFEQQKQLERQAILRDSLKIDELKKQIAELQAQLQTPQITEDQ
                     RQVTEQQTTTREAELESLREQLRESNRRLQELELSSLETTPPSMLRVRNYGMWYAIIA
                     AIVLLIILLIVLLVNHRKQVKRQELEWGYGSQIPMGPRPTQPILTRQPEETPKPQSPV
                     PPITQPIATPAPPVESAQPAQPSAP"
     assembly_gap    31453..31552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            31715..32647
                     /locus_tag="PLO58_02195"
     CDS             31715..32647
                     /locus_tag="PLO58_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HQO73632.1"
                     /translation="MIDAKNLSGLDKATILFKALGPDLSVQLFKGLSRSQMQKIRENM
                     AKITNIPFDVKKAVLEEFYFSFVSEKFTPAEEETKKPFEFMLKLTDEQIAYLIASEPP
                     IIMALAIAQLPVERQVNVLQKLDPTVQPKVLTEMGHLTEIPLEGVVSIANNLKDKSAF
                     IPRASEFKRGGGQDVAGILSKIPPRDEKRFLEFLSKEAPELVDQIKRYYFSFEDLPKL
                     PPNILAEVLKSVEISEIAYALKGQPDEIRDLFKSLLPQRSQIILEDEMKLLEGPQPRR
                     KVEAAQKKIVEKLRELEKEGRFNLEDFMDTDFIE"
     gene            32739..>33339
                     /locus_tag="PLO58_02200"
     CDS             32739..>33339
                     /locus_tag="PLO58_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73633.1"
                     /translation="MKIIKTLLITLLLGGFANLKSDTFEQQEERVQIALKAMYNREYK
                     RAASLFHEVLNSTPFHPMGPLGALAVEYYQNEGLIGYHQRNQQFLVDIEQALQIYADQ
                     IKQNPSHSEYFFFYGTVMGLRARIQLAEKNYFGVLTSGYNAIRFIKKTEKLYPDQPDF
                     QLPVGLFNYYVSISAPYMKIASRLMRESGSRSEGLQAIKA"
     assembly_gap    33340..33439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33440..33696
                     /locus_tag="PLO58_02205"
     CDS             <33440..33696
                     /locus_tag="PLO58_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73634.1"
                     /translation="YGSLALAQGRLDEAESNLIEFIKSYDYELDYDLGNALVRLGNVY
                     DLQKRRSEAIACYRKAVQLNNRTAAVSLANQYLKQPYNRN"
     gene            33885..34736
                     /locus_tag="PLO58_02210"
     CDS             33885..34736
                     /locus_tag="PLO58_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQO73635.1"
                     /translation="MNGQIPVLMVNGESLAAAWEKSLVELYQKGCDIKTEYDKPSDPP
                     SKDCTMMIVVQNPLAEPMIHCDFPGGLTDLQEYVLEVLEGIKDHCIRSAENPADARWE
                     YTYHQRLFNYQVPGFAKVYDQIAIMVAKLIKVPHTRRAQAITWKVWEDNDCYDPPCLQ
                     SIWCRLLKDDNSIWTLNTNVRFRSNDAYKAAFMNMFALTQLQVEIANRIEQSLGEKIN
                     IGRYVHQADSYHLYGSYFDEFQNRFLKALETRSFEERTFRYADVKSIMTEAIPQIRAK
                     AARMGRE"
     gene            complement(34939..35748)
                     /locus_tag="PLO58_02215"
     CDS             complement(34939..35748)
                     /locus_tag="PLO58_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQO73636.1"
                     /translation="MQWFGELSPVFQAFLATCFTWLVTAGGAGLVIFFKEVSQRLLDA
                     ALGFAAGVMIAASFWSLLAPAIEMAGASGSPAWIPAAVGFLLGGAFLKLVDRLLPHLH
                     LGLPIDQAEGIKTNWQRTVLLVLAVTIHNVPEGLAVGVAFGAAGAGLPSASLAGAVAL
                     ALGIGIQNFPEGVAVTVPLRREGLSIGKSFFYGQLSGFVEVISGVIGAYLVLTIQALL
                     PYALSFAAGAMIYVVIEELIPESQKDVSTDVATLGAMLGFTIMMILDVALG"
BASE COUNT        10291 a         7829 c         7337 g         9779 t
ORIGIN      
        1 acgcaaacaa gccgaagaac aacgcgaaaa atttatcgcc gagttgcagg aagcactcga
       61 caaaatcaaa accctgaaag gcttaatccc gatctgcgcc tgctgtaaga aaatcagaga
      121 cgaccagggt tattggaata gtgtcgagtc ctacattaaa gaccatgctg atgtggaatt
      181 tacccacggc atttgcccgg attgtatgaa aaaattatat ccaaattact gtaaagacga
      241 taacgataaa aagaagcctg agacaaaata gtctattcca gacgttttat cagaatctga
      301 tagccatcgg ctttgacgac tttattataa aattctttga atgcaggata aatttccggc
      361 gtaatctcgg attccttatt tatcattagc cgagcgcctt ttaaaattct atcattataa
      421 tccagcgtaa gccgataatc ggcaatcgga caggaatatt cgtaatacgg agtaaattcc
      481 aacggttgat acccagccgg gaaggtgatt gtgatttcct cgctaatcgt atcggcattt
      541 ggccagtaag taaacggata atttctttta tcatacgata gcgccctaat tggcgaaagc
      601 cgatcagccc aggggatgcg taaaatgaga aattttccgg cttccgttaa ataatctgga
      661 accttaaaag tgtattcata atccaaagtc ggtttaattg agtcgatgtc aaataaatta
      721 aagctaatca gttccacgtt gggaaaatct tgagccaacg attcgattaa ttctttgtcg
      781 cgatcggctt ggctcttgaa tcgataaacc gcacgaaatt cagccgtctg agcacctttg
      841 cgagtggtgt gaagctcgac ctgaatacta ttatcggttt gaacctcgac tttggtacgt
      901 cgttgccgaa cactccccat aaaaaagtgg cgcgttagat aaaacggttg gcaacccttc
      961 ttgatttcga gcgcaaaagc cccttgatcc gccaagggca ccgaaccgac cggaaaattc
     1021 tgcgctgtca aatcgagata gagcgttccc tcttgagtgg caacgctgac gatggcatga
     1081 ttaaaggcta tcgaaggcaa tacgtgttta ttcaaacctt catagcgagc gtttaccaac
     1141 acgaaattgg ctgtgaatcc cgctgagcgc aacatagcga ttccgagggc ggctttatcc
     1201 ttgcaatcac cgatgcgggt tacgaataca tcgcgggctt tttgaggaat caaaccggac
     1261 tggcggaagg atacactgct atagcgaata ttttcggtga tataattata aatacgggta
     1321 attttctcct gttcggtcag cggttcattt tccggtaata aagtcttgag aaattcgtca
     1381 atctcataac atgaacgagt tctggtttct gccagatcaa tataccagtt cacgatgtaa
     1441 tcccagtccg gaatgctgga aatatgcaat atttttccga tatctgctag gggaggcata
     1501 ttgtattcct cttttatggc gggaacgtcg ttcaggcacc attcgtagag aattccatcc
     1561 ggcgtttccc ggatagccga aggttcatcc ggcataaatt gacctttata ctgaaatttc
     1621 acattcggcg gaatcaataa ggcatagcaa acttcccgca caggaaaaaa tgtattgaaa
     1681 ctgaaatcgt cccagaaatg ctgggatagt ttgccggagt aataattgcg aattttccat
     1741 ttaaggtaaa cggcgtcgcc gggttcgagt gatttaaata caatatagcc ccagctaacg
     1801 tcagcatcaa ttttggagcc atcctttttt aaagtttgcg ccttttcgac aatcagttct
     1861 tcgttataag cgttataaga aatccggtat tctttgaaag catcgattcc ttccagatta
     1921 aaaaccgtcg ccagaatttc ctggctcatt tcagttgcgc caccataaac gacacgccga
     1981 caggcattgt atagaataac cgccttttca ccgggatact gctccgacga tgggcaattg
     2041 cgaaaaagac tatcaatatc caccgtatcg aaattgttaa aaattgatgc gcgtccttgc
     2101 aattgcctta atttatcgcg cgcatcataa taagtcggac tcaattccag cgctcgctgg
     2161 taggacttaa tagccgcggc ggattgaccg gacgaacgtt ggatttcggc gcgagtgccc
     2221 cagtagccgg cacattgcgg acagatttgg agagccttct cgatataatc caaagcggtg
     2281 gaatagttat gcctattata gaaattttgc gccatttgat agaaaaggct tggagaagcc
     2341 ggataatatt caatcgcctg tcgataggtt ttttcccagt tcttaaggtc agcggctttt
     2401 ttataataat cggctaattt gagaaaagcc gtctgctcgg tgtggttttt cacgtattta
     2461 cgtaaaatgg gaatagcggc tttgttattt tgtttaacgt aagcttcgag attggctttg
     2521 gtttcgacaa tgttccagtt atccgggaat tgatggtaag cggcattggt tatctcgatt
     2581 accttctcgg tcagaccgcg tttggaataa taagcgataa gttgcaattg tagattgggc
     2641 gaatccggta tcaattgcgc cagtcggtcg agcaaattct tggcttcatc ataatttttc
     2701 tttgcgagga gttcagagat tcggtattct aaaacgcctg gaatatccgg atcaagctgg
     2761 taaatttttt cgtaagcgga tttagcttcg tcatattttt caccgcgcaa gtaagcttca
     2821 atcaaatgta aataaatcat agcattagtc ggagcgcgtc gcagagcctc gcgtaaaatc
     2881 aattcggact cgactgcttt atcgtttaaa gcatagcatt ccgccagcag aagatagttt
     2941 tcgagatagt cgggatgttc tgcaattttc gtcagaaaaa attcctcagc gaagttcggg
     3001 atgggctctt tcacggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggtg
     3121 aaaaaataaa agtattgccg taaaaaactg catcgttatc ggcaaagtac cgacgcatat
     3181 caatccattt gtcataacgc acttgcggaa tccggaacca cttaatcgca atatcatttt
     3241 gtccgtgata aactgccgcg gtgtcgaatt cggtctctgg cggatagacc ttatcatatc
     3301 cagaagccga aatattatcg aatggaccaa tcaacatcca atcggtgatt gcgtcaattt
     3361 tatcgaagta aaatagcgcc tttttcagat tagcgtgttc gttatagtag tgccctaact
     3421 tttcattggc gttgattcgc atcgtagaat ggcgagcagt gcgcgctaaa ttatcgaaga
     3481 gcatctggat accagctgat ttatattcct cggcgttggt taatcggggc gccatcatag
     3541 cggcaaaaat ataaggttca aaatcggctt cggtgtcaat gactttggcg taggtttgcc
     3601 aggcttccag atgtttatat tgcatttcat aaaggtaagc taacccgatg taagcacgcg
     3661 tgtcgttagg attggcttgg atggcttctt gaaatttctg ttcgacgagt ttctggtcgt
     3721 ttcggttcca ggcttgccag gcttcctcca catattgagc actcgtgaaa atattagaga
     3781 aaagggactc gccgaatagc agaataatta atatggtatt tctaaaaaat cgggtagctc
     3841 tcatttttat tttcctgttt ttaggcaatt tcgataatat aaattaacaa ttcccatact
     3901 accgaataat aagtcttttt gccgagaaat gaaacgattt tatgatctat ctaaatcggg
     3961 aaaaattgtg ctgtttttta agacaaaccc tctatggaat ttccacctgc cagagtaaag
     4021 aatcctgcgc ttggcgcgga ctatgcaacc agaccgccaa atgatagcgg tcaccagaat
     4081 aaccggtgaa aattgttaga aatgtatagt aacaaataca tttgcaatag catttttcgt
     4141 ggggtatctc accacaggta tctctaatca taatcttgag actgtcaccg gactgctcac
     4201 tttcgacatc gaacggcgca cagcaaatgt aggtaattcc ggtaaaaatc tttagcgtat
     4261 cgtcgctgat ttcccagctt agtgaatcat tttgaatcgc cgcttttggt aaaatgtcac
     4321 cccccttgta ttcattacat ccgccatatt cggtccataa taacgaaaga gtggcggcag
     4381 ttggtggttc actgccatta tcattacaag ccataaatcc gacaattaat attccccaga
     4441 ttagtaggac tttgaattgt tttaacatag tcacctccta attgcgactt tgctcaaaga
     4501 taaacaaaaa cctaaaatat ttaaacaatt atttattcaa aaccgagtaa tgggcgatta
     4561 aattttttcc gaattttatt gatgccctgc aaaacgcccg gtctttggac taactttctt
     4621 tcgagcctgt atttacctgg aaaattcatt aaaattatac cgatcataat cgttaagata
     4681 ccctgccctg ggataaacaa aagtaagaat ccgactatca ggaaaataag tccgattagg
     4741 tttttgacta tcagtaatat tacacccaag agcggtcgct tggtaatcaa gaagctttta
     4801 cggcgtttgg gattggaaaa ataatccgtc ggaatcatca gaatcagtac ccccattaaa
     4861 agaattgtac caaaaaatgt gatcaaagaa atcaaaccgg catatttcaa caaagtcgta
     4921 tgcgctttaa gccaatcata aagtagcgga aaacgttcca gaaaattcag agccatagga
     4981 caatcgggga ttaccggaaa acaaaagatg ttatagattt catcacgtta aaaataattt
     5041 aaaatgaaat tttattgaac agtttgccct attagatata cgcattaacc gagttgtttt
     5101 attaatgatt atatctctaa ggttcaatgc cgagaataag aagcggtaca ggagggctgt
     5161 tccgtccttg tctcataaca ttatagactt ctccctcgta atatgcaatt ccgcttgaca
     5221 tttgggactg catattctta gtaggaagaa tttctatgcc taagtttatc atgccgcctg
     5281 aataaaatag catgtgcttt acaaacaaat catatgcaac aaaagcatat ttaccaaatt
     5341 ggacttcaac agcaacttta tttttgacga aatctgtctg attatagcta aaaattgggt
     5401 ttttctcccc gtgttctatg agaaatttct tttgttcacc tgcaggcatt aataaacttt
     5461 tttccatcag ttcccgattt agagtgatat agtaattata acgactctcg ttccaatttc
     5521 tttttgaaaa ttccctgtca aatgctttgt ttagttctat tggactatat aactgtgtcc
     5581 ccgcttttct attttcctta ctgacttttg tttttaatcc ctcggcattt atgttcttga
     5641 taacactttt gatttcatta taaagatctt tatgatgcac aatcaaatat tcttcgccgt
     5701 tcaaatgcga atatttttta attattttca tttgctatat ccgttaaatt ttaattttag
     5761 ccattcattc ggcacattcg caactttatc atttttagaa ggtttgtgaa taggtttatt
     5821 aatcggtctc acttttaagt tacctgcttt caaagcttcg attctatttt gggcgatttt
     5881 acaataatgg gcttcttttt caataccgat gcaattgcgc tcgtttttaa tcgaagctat
     5941 aattgttgaa ccaacaccag caaatggatc aagaacccag ctacttttat tagttaaggc
     6001 taaaacacag cgctcgacta gttcaaccgg aaattggcag ggatgttctg ttttctccgg
     6061 atggttcgat ttaacgtttg gtacattcca tatttcatta tcccaatctt ctacaacaat
     6121 tttccagata tccgaaggat ttttcccaag tggatttccg gaaggctttc ctttgttagg
     6181 tcctttaaaa tgtaacttgc ccggatattt tgcaggaatt ctaacggcgt caagattaaa
     6241 aatgtaatta tcggttttgg taaaccacag aatggtttca tatcttcctg aaaatctttt
     6301 ggaagcatgc aatccatgct caaaatgcca gataatacga ttgcgaagtt ttaattgata
     6361 tttcttgaat atcggataat agtaaatatc aagcggaaat atttctccct tatctacaaa
     6421 atttcctact tgccagcaaa gactcccctg gttagaaaga acgcgtatta gctgtctaat
     6481 aatttcttct tgcgtttcaa gatatttctc gatacaggtt ttggtttcat attccttacc
     6541 aatattataa gggggagaag tgattattaa atcgaatttc ccgtctggca aattttggag
     6601 gatataacaa gaatccccat ttaaaatttg atattcaatc gcaggattta cataattaaa
     6661 tagagtttct tttacttcca tattattcgt tttgtttaaa aactctgatt attttacgta
     6721 aaatttacct aaaaggcgaa taattaatct tcagaaattt aaaaggtcag aggataatga
     6781 tcaacgcttt acaatcccag aattttttcg atcggccgcc gttccggtac ataccggtca
     6841 ggaattttac cggcattcca atcggcggaa acatacaaac cgcagtaaca acgcccatat
     6901 tccttcacat ccggttcgcg gtaaacgcat gggcaaatga tatcacgatc cgattcgcga
     6961 tcaccgaccg ccagacgaca tggacaagcc atataaccat aacgttcttt gttgatcaat
     7021 aaagaattca gcaatcggtc aacgtgctga cgatctttat tgaaaaaata gcctttcggc
     7081 tcctgaagtt tgcgaagcga ttcaaataac tgttctgtgt tcataatccg agtgcctttt
     7141 tgatttcttc cggacgatag ccgacaataa cgttgtctcc gatgacaatt actggaaaag
     7201 caactgtcgg tgttaattta tgaatttctc taatgactgc cagtttttgt tccttgtcta
     7261 acaaatctac atcaatgcta tcgtactcga ttcccagact cttgaggaag gctttggtat
     7321 tctggcaata aatgcaggtg ctgagagcgt aaagtttgac ctttttatcc ataaacgact
     7381 cctttcttcg gggctaattc tgcggataaa ttttatttcg gtgcttttga gataatttca
     7441 caatgatatt gcaatctgat taccaaaact aaaacaaata taagcgttta aaattacaac
     7501 cggtaaaaat attcttaaag cggctttgaa agtttctctc aatttgatac tggctttcgg
     7561 ttgtttgaga tgatcgaagt cgccggtctc agggttaagg tggtaatctt ttccccactc
     7621 aacataattt ttcataattt cccgtaccgc agatgcgata taacgcactt cgacttcagt
     7681 catagtcgga tgtaacgaaa tgcgcaccca gccgggcttc agactcaagt tgccctgatc
     7741 aatctgactc ataattgctt tggatcgttg gtcgtcaata tgtagtaaaa catgaccgta
     7801 agttccagcg cagagacagc cgccacgtgt ttggataccg aagcgatcat taagtaggcg
     7861 tactagcaga ttatgatgaa ctttcagact ataaaacgaa acaaaaccga tgcggttatg
     7921 gttttgagat tcaagaatgt ttatatcagg gattccagcc agttcattta gcaggatttc
     7981 ggtcaagtaa tgttcctgtg ccagaatatt ttcaacgccc atttcttctt tcagtaaaat
     8041 tgccagcgcg gcgcgaattg cctgtaaaaa tccgggtgtt ccaccatctt cgcgcacttc
     8101 aatgtcctgg aaaaaacgat gttcgcccca gggattagtc caaagtacgg tgccgccgcc
     8161 cggttgatct ggaacgcgat tattataaag ctttttatcg aaaacaatta ctcctgatga
     8221 gcccggtccg cccagaaatt tatgcggtga aaagaaaatc gcatctaatt tctgttccgg
     8281 tttgggcgga tgcatattga tttcgacata gggagctgaa gcggaataat caataaaaca
     8341 caggcgtccg aattggtgca taatctctgc taattcgtta accggtgtga ttaagccggt
     8401 aacgttcgag caggcggtga atgagccgat aattaatttc cggtcacggt ttagttctaa
     8461 gatattacgc aaatgctcaa gattcggtaa tccgtccgat tttttacaaa tgagtgaggt
     8521 gtcacagcaa cattcttccc aggtggtttg gttggagtga tgctccatat gcgttagaag
     8581 taccagcgct ctttcttgag gcggtattga tattttttcc ttgaatttat caggtacacg
     8641 taaccctaaa agacgttgga atttattgat aaccgccgtc atcccgaacc cagccagtaa
     8701 taggacgtcg tccggaccgg cgttcacatg ctgtttgata atcttatgcg cttgatggta
     8761 ggcatcggtt atgattgtcc cagtcaaggt cgcatcacta tgagtattgg caacgtaagg
     8821 gcctaaaaca ttagttatat attcctcgat cggtcggtag agtcttccac tggccgccca
     8881 atcggcatac agaatcggtc ggcgaccgcc cgggaaatta tactggtaat caatgccgat
     8941 aatattacgg cgaaaacagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     9061 ctgccaatta tcattaagta tgcaaatttt cggaaagaaa tccgcaaaat aaactgaata
     9121 actaaatttc actttcgatt gttagaagta attagcatta acatatttta ctacgtttta
     9181 gtttaaatta aagacgtatt tctggtgaaa attaattgtc tctggttcct tcttatttta
     9241 aattaaaatg ctatgaacga atttaaacta ttgaccaatt ttaagccgac cggcgatcaa
     9301 ccgcaggcga tacgggaact gaccgaaggg atattacgag gcgacaagtt ccagactctg
     9361 ctcggggtaa ccggaagtgg taagactttt accatggcac aggtcattca gaacgtgcaa
     9421 aagccaacct tggtcatttc ccacaataaa acacttgccg cgcaattata tggagagttt
     9481 aaggcgtttt tccccgaaaa tgcggtcgag tattttattt cgttctatga ttattatcaa
     9541 ccagaggctt atctgccggt tacggatact tacatcgaaa aagactcctc gatcaacgat
     9601 gaaattgata aactgcgcct caaagctacc agctcgttaa ttagtcgtcg cgacgttatt
     9661 gtagtcgcca gcgtttcctg catttacggt atcggctcgc cggaagagta cactcgcttt
     9721 attgttactc ttaaaaaagg tgaccggatt ggcatccgtc agcttttcgg ccgactggta
     9781 gatatttatt atgctcgtaa cgatttagtg ttggagcgcg gaaaatttcg cgctcgcggc
     9841 gacatcgtcg agattcgtcc ggcttatgag gattacgcct accgcatcga actatttggc
     9901 gacgagatcg agcatattta tatctgcgat ccgttgaccg gcgaagttac aaacgagcaa
     9961 gaaatggtcg taatctttcc ggctaagcat tttatttctt ccgaagaaac gattcagcgt
    10021 gccattgttt ctatcgagga agagctagag gcgcgccttg aagaattacg ttctgccggt
    10081 aaattattag aagcccagcg cttgggacag cgtactcgct tcgacctcga aatgattcag
    10141 gaagtcggtt actgcagtgg tatcgaaaat tattcgcggc atttctccgg tcgtaagccc
    10201 ggtcagcaac cttatactct gctggacttt ttcccgaggg attttttact aataattgac
    10261 gaatcgcacg tcactttacc tcaattgcgc gctatgtata acggcgaccg atcccggaag
    10321 gaagtgctgg tcgagtatgg ttttcgcctc ccgtcggctc tggacaatcg tcccctcaaa
    10381 ttccacgaat ttgaagagat ggtcaaccag gcagtttttg tctctgccac tccctccgat
    10441 tacgaattgg aaaaatgcaa cggcgtagtc gtggagcaga ttattcgtcc gacaggtttg
    10501 ctcgatccgg agattatcgt aaaacctacc aaacatcaga ttgacgatct aattgacgaa
    10561 attgcggctg tcaccgctcg caaagaacgc gtgctggtca ctacgttgac caagagaatg
    10621 gcagaggatt tatctgagta tctgcgcggt atgggactta gagtccgcta tttgcattcc
    10681 gagattaaag ccctcgaccg cgtcagcatt ttgcgcgact tgcggctggg aaattttgat
    10741 gtgctggtcg gcattaatct cctgcgggaa ggtctcgact tgccggaagt atcattagtc
    10801 gcgatattag acgccgacaa agaaggattt ctgcgttccg agaaatctct gatgcaagtt
    10861 tcgggtcggg cggcgcgcaa tatcaacggt cgggttatct tttacgccga tgaaatcacc
    10921 gaatctatgc gcaaagtcat cgaagaagtc agccgccgcc gcaaggttca acaggattat
    10981 aacatcgccc acaatattca gcctcacacc atttataaat ccgtcgcaga agtaatgcag
    11041 accaccgccg tggctgacgt taaaccgact ccaatccaga ccgaccagcc ggttggcgac
    11101 cgctaccgcg ctaaaatgga aacgtctgag atcatcgaac tacttcgtcg cgagatgctc
    11161 gccgccgctg aaaacctaga attcgaaaaa gccgccgaac tgcgggacga aatcgcccgc
    11221 ctcaccggcc agaagaaaag cctatctcgc gaaaatctgc tttagatttt tccgactaaa
    11281 ccgatttgcc caagtacaaa atttttaaat gaattgctta tgtaatccaa ccttttcctt
    11341 gtttcaataa tcaaaactcg gtagattact acgacatttt gacttactct aaaatgatta
    11401 acatttttat tttaataaac tttaagatga attaaatgaa agttctaatc gtcggaagtg
    11461 gtggtagaga acatgcctta gcttggaagc tgtcacagtc accatatata gctaaaatct
    11521 actgtgcgcc cggtaacgcc gggactgccc ttttggcaga aaatattcct cttaaagaca
    11581 acgatctgga cgggctacta aaattcgcac tcgagaactc tatagattta acggtagttg
    11641 gtccggaaat cccactggta aatggcatag tagatctatt tcgtgctcaa ggtttaaaga
    11701 ttttcggccc aacatctgcc gccgcccgac tggagggcag taaagtattt gccaagcaat
    11761 ttatgagtcg tcatcaaatt ccgactgccg cttaccaagt ctgcgattca cgcgccgcaa
    11821 tggaatctgc aactcatttc aagacttttc cctatgttat caaagttgac ggattagcgg
    11881 ctggtaaagg cgctctaata attcacaacg acgatgactt aagagaagct tttgaggcaa
    11941 tctgggatga gaagcgtttt ggtaccgctg gaaataaagt cgtagtagaa gatttcctgc
    12001 tcggcgaaga gctctcggtt ttcgttatca ctgacggtga acactatctt atcctaccac
    12061 cagctcagga tcataaacgc atcggcgaaa atgataccgg acctaatacc ggcggtatgg
    12121 gtgcttacgc tccggcgccg cttggcactc cgcaagtaat gcacaatgtc gaaaaacaaa
    12181 ttatcattcc aactttgaat ggtttaaaag ccgagggaat tccttatacc ggtgtacttt
    12241 attgcggctt gatgattaac ggaactcagc cttatgtggt cgaattcaac gctcgctttg
    12301 gcgatcctga gacccaggtg atattacccc taattgaatc ggatctggga gtgattttac
    12361 acagcgcagt tgaaggaaag ttagatgcca cgatgatgaa attatcccga aaaacggcag
    12421 tttgtgttgt tatggcttcg gccggttatc cgggcgacta tgaaaaaggc aaggttatct
    12481 atggcttaca agatgaaaag ccagaatcag atacgattgt atttcaggcg ggaacaatcc
    12541 gaaataatgc ccactttcta accaacggcg gtcgggtatt agcagtgact gctctggcag
    12601 ataatctcga aaaagccatt gccagagcct atgagcgagt cgccgaaatt acctttgtgg
    12661 gcgcttacta ccgccgtgac attggtcaaa aaggattact ccgccaaaaa gatttattgg
    12721 aaaaaattta agaaaagaag caaaaaatgg acaacgaacg cattaataaa ctacagcaaa
    12781 gtttcggcat tctgggcgaa tccgattaca ttaaggaaat cgtcgaaact attgatcaag
    12841 tagcgcgcac cgatattacc gtcctgattt gcggcgaaag cggcaccggc aaggaggtcg
    12901 tcgctaatgc tatttatcgc cacagcttgc gcaagcatca gcccttcgtc aaagtcaatt
    12961 gcggggcaat tccagccggg attctggaaa gcgaactctt tggtcacgtc aagggttctt
    13021 tcaccggcgc aatcgaagac cgcaaaggct attttgaaac tgccaatcac ggcacaattt
    13081 tccttgatga aattggcgaa atgccgccag aaactcaggt caaacttctg cgagtgttgg
    13141 aactcggcga attcctaccg gtgggcggct ccgaagtacg ccgcgtggat attagggtca
    13201 tcgccgcaac caataaagaa ctcgccactg agatcgagcg tggaaatttt cgcaaagacc
    13261 tttattaccg cataaagact atcaccattt atatgccgcc cttacgtaaa catccggaag
    13321 acatcccgct tttagttgaa aaatttgccc tggacttcgc taatcgcaat tcgatcgctt
    13381 ttaagggttt ttcgcccacc gctctgaatt tattgcgcga atattcctgg ccgggcaata
    13441 ttcgggaatt gaaaaatttt gtcgagagta tgatcgttct ggaaaaaggc gaggtaatca
    13501 ctagtagtat ggtcgagaag catttgctgg gcgagccacg ccacatccct tcgcctcatc
    13561 tgccgatgaa aattagtaaa agtgtcgatc aagccgagcg tgagttaatt ctccaacaaa
    13621 ttatggtact ccggcaggaa ctgcatgaaa tctatgaagc tgtgattacc aaccctcttc
    13681 cattaaaata tgaagccttg cgcgaattgg aaataccacc cagtctgccc cgacccaaag
    13741 tcgaagaaaa agaaatcggg aaactagata acgccgaaat cattaacgac gagattatca
    13801 atcccgaaat ggtgggaaaa gtaaccctcg ctgaagtcga aaaggaatta atccagcgca
    13861 ctctcaaaaa attcaactat cgcaaacgcc aagccgccaa tgctctccaa attagcgaac
    13921 gcactctcta ccgtaaaatc aacgaatacg gactggaaga atcaaggtaa tcgcaatgct
    13981 attcaagcgt ttggctccca tcataaaatt tattccagcg gttttattat gtttaataat
    14041 caactcctgc tggctttatt catttaaagg caccttgccg cccgacatca aaagcattgc
    14101 catcccgctt tttgaggacc gcacggctga gtttaatatt caacagatag taacggataa
    14161 gatccgactt ggttttatca aagaaaattt actgaaagtt gtcgaagagg ataacgctca
    14221 ctcagtccta tacggaacca tcttgagcat tgatgacaaa cctctggttt atgacgaaga
    14281 tgaaaacgtc ggcgaatccg ttaccgaata tcgggtaaca atcaagatcg agatcgagtg
    14341 gtacgataaa gtcaatgacc gcacagtttt caaaaaacag ttcatcggct atagtgaata
    14401 cgacccgacc ggcgccacat cccgaacgcg tgaggtggct ctaagcgaag caattaatca
    14461 aattacagaa gatattatga acacaattct ggcagattgg tgagtatttt atcggttata
    14521 gataaaatta aatagaatct gatcggactc gaaaggggca aaaatgcaat cggttttcat
    14581 taaagacatt caaaattaca acaatcaaga agtaaccttg aaaggctggg tttataatct
    14641 gcgttccagc ggtaaaatct ggtttttact cttgcgcgac ggcaccggct tgattcaggg
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccagcggtaa aatctggttt
    14821 ttactcttgc gcgacggcac cggcttgatt cagggtatcg tatcacgcaa tgatgtttcc
    14881 gaaaaaattt tcaattacaa ggaaatcctg actcaggaat cgtccgtcag cgttagtgga
    14941 ctcatccacg aagataaacg tgctccgggc ggttttgaaa tcatcgtcaa agatattcag
    15001 gtctatcaaa ttgccaaaga ttatccgatt acccttaagg aacacggcat tgaatttttg
    15061 atggataacc gccacctgtg gctccgttcg cgcaaacagt gggcgattct gcgccttcgc
    15121 cacgcggttt actatggaat tactcaatat ttgaatgaaa acggatttat ccgtttcgac
    15181 tccccgattc tgacacccaa tgcctgtgaa ggtactacta cgcttttcga agtgccttat
    15241 ttcgatctcg gcaaagctta cctttcgcaa tccggccagc tttatctgga aaccgggata
    15301 atgagtctgg gtcgggttta tgacttcgga ccggtctttc gtgctgaaaa atctaaaacc
    15361 cgcaaacatc taaccgaatt ctggatgatg gacgccgaag ccgccttcgt tgaacacgaa
    15421 gaaaatatgc gcattcagga agaactcatt cgcttcgtaa tccgtaaaat tctggcagaa
    15481 tgtcaacctg aactcgagat attggaaaga aatatcgaac aattgcgcaa agccgatgcc
    15541 cccttcaaaa aaatgaccca ccgcgaagca gtcgaatatt tacgtagcaa aggctcatca
    15601 atgaacgaaa cggaagattt gggcgctcag gatgaaacct tactgaccga agggtcagat
    15661 gtcccgattt tcgtcgaaaa atggcctaaa gaaatcaaag ccttctatat gaaacgcgac
    15721 tcagaaaatc ccgacctcgt actcggcgac gacctcatcg ctccggaagg gtatggcgaa
    15781 attattggcg gctcgcaaag ggaagacgac tacgatttac tgctccagcg tatgattgcc
    15841 gagaatatgc cgctcgacga atatcagtgg tttctcgacc tgcgtaaata cggctcagta
    15901 ccacacagcg gctttggcgt tggactggaa agattgatca cttggattgc cggggtgcca
    15961 cacatccggg aaactatacc atttcccaga acgatttatc ggctgagacc atgatgaccg
    16021 tttcaccaaa aatcatcgcc gttctgggtg gccgcgaatg cgacgaacaa acctatcaat
    16081 tggcttttga tgtcggtcgc gccatcgccg aacgaggtgc gattttactc tgcggtggac
    16141 gcactggcgt tatggaagcc gcctgcaaag gagcagtttc agccggtggg ttgaccattg
    16201 gcattttgcc cggcgcagat aaaaatgacg ccaatccgtg ggtgaaaatt gccctaccaa
    16261 ccggaattgg tgttgcacgc aatgctatca ttgcgcgtgc ttgtgatgcc gccattgcta
    16321 ttggtggtaa atatggcacc ttgtcggaaa ttgcctattg cttgcaattg aataaaccga
    16381 tttgcggatt aaattcctgg ccggaaatta gcgatctgcg ccagtttaat agcgcttccg
    16441 ccgccgtgga atttgctttc ggggaataaa tatatggcga tacgcgttgc gattatcgtt
    16501 caaggcgtag ttcagggagt tggtttccgc tggtatgtcc gtcggattgc catgcgctac
    16561 cgcttgaccg gttatgtcaa aaatttaccc gacggagacg tttattgcga agtcgaaggc
    16621 gaggaaggaa tgatcaatga ctttatcagc gaactcaaag ccggaccacc attaagtcac
    16681 gttactaatg taattgtcaa taaattaccc gacttggtcg gctataaaag tttcgagatt
    16741 agattttgaa acaaataata aaaatcggcc tggcgcagat taatcccgtt ctcggtgatt
    16801 tacccggcaa tgctaaaaaa attaccaact ttattaaaga ggctgatcaa cagggaattg
    16861 acttattgat ttttccggaa ttggcaatca ccggctattt gccgcaagat ttattgctga
    16921 accgctcgtt tattgattgc aacttgcaat gtttgaataa aataactgcc gcttgccgta
    16981 gttcgatggt cgtcattctg ggctacgtcg ctcgggaaaa tggcaaactt tacaatgccg
    17041 ccgccgtcat taaatccggt caattgattg caacgtgcta taagacactt ctgcccaatt
    17101 atgatgtttt cgatgaacac cgctatttca cacccgctaa tgaaaacctg ccggtaccaa
    17161 tcgaaattgg tggtcattct gtccggctcg gtgttgaaat ctgcgaggac ttgtgggata
    17221 gtaacagcgc tatcaaagta accgatcaat tagttgctca gggcgctgag ttattggtca
    17281 atttatcggc ttcgccattt gaatatgata agcgcgaccg ccgtcggcaa ttagtactcg
    17341 acaaagtaac caaactcagg cgaccatttg tactggtcaa tctggttggc ggtcgtgacg
    17401 aactggtttt cgatggcaat tctttcgcac tggacgcctc cggggcgtta atcggttggg
    17461 gcggagagtt caaagaaaac ttagccgttt tcgagctgga tttggttagc ggacaaggcc
    17521 accgaatgcc ctggcccgaa actcaacgcg aagccagcat ttatcaggct ttgaccctcg
    17581 gcgtgcacga ttatttctat aaaaccggtg ggcgacaagc gattatcgga ttgagtggcg
    17641 gaattgattc ggcgcttgtt gcctgcatcg ccgtatccgc tctcggggct gagaacgttc
    17701 taggagtatt tctgccgtcg caatacactt cggccgcaag cagagaggat tctgttgttt
    17761 tagctaaaaa tttgaatatc agattaatcg aaatcccaat cgaaaaaata tttcagtctt
    17821 atctccagga attgaaaccg gttttcggcc aaacgtcacc agatgtcacc gaagaaaata
    17881 tccaatcgcg cattcggggc aatttactga tggcgctggc gaataaatac caggcttacg
    17941 ttttgaacac tggcaataaa accgaactgg cactcggtta ttgtacaatg tacggcgata
    18001 tgtgcggcgc gctggcagtg atttccgatt taagcaaatt agatgtttat aagatcgctg
    18061 gttatttcaa tgaaacggcg gaacggctaa tcattccgga acgtattttc accaaaatac
    18121 cgacagctga attagccccc ggacaggtgg acccattcga ttacacgatc gtcagcccgt
    18181 tggtagattt aatcatcaat gatcaaaaga ctccagcgga attaattcag atgggttacg
    18241 cagctgacct ggtaaacgat atttttgagc gcattaaatt tgccgagttt aaacgccgcc
    18301 aagccgcgcc cgggctgaaa atcaccagta aggcttttgg tttagggcgg cgttatccaa
    18361 ttattaatca ttttaaagaa aagggaggaa agcagtgcga gtagttcgct attggctgac
    18421 attaatttgt ataggaatag ccttgtatac cgcctcaccc cttcttgccc aaaatccaac
    18481 cgacattgac ggcacaattt ggcggcggat gaaatatgat aataaatatt actggctatt
    18541 gggttatctg caaggactgg aaaaatcgga cgaaatcatt caactttgtc tcgctgagca
    18601 ggaaaaacgc gaatttgcct tcaccgttcc tttttatgta gatcagatgc gcgcccggat
    18661 tagtgcttat tttccgcctc ataatattca agacatcact caactaattg aactgctgaa
    18721 tactttttat aaagacaaat acaaccagaa gatcaagatc gaagccgcgc tccgcattgt
    18781 cctagctcgc caaaaaggac agattgaaca gtctgatttc tggctgaatg aagcccgacg
    18841 cgcgctcatc caaaaataat tccaataatc ccggagaagg tacgatgaac gaccataccg
    18901 aatttgaaga aataaatctg gcgcggcata tctatcgtta tttgattaca atcggcattg
    18961 ctttattcgg attattctta atttctatcg aaccgaatgt cctgcgccag tatcgcagtt
    19021 ggattccctc ccaaactttt aatatttact atctgacctg gttcgcagtt tgtaccattt
    19081 cggcgatact ttgctttttc tggggagttc tctgccagtt tttacgaggc aataattgtc
    19141 tgggaaatgt cctctggtat gttggcattt ttactataac actctttttc gccaccgtat
    19201 ttttccagat tttaaaaacc gatagatcag ttggattggc ggtaactttt ggaacattaa
    19261 taatttccgc gatggttaca atggcgatat cctatatttt ctaccttttg aatttcaaca
    19321 gccgttatca tttttggctt ttcacattgg ctattatttc ggctaccagc gcttataatt
    19381 ggttatactt gagcgttatt ggcaagattc cggcgtttta acttaaatcg tcgtttgtca
    19441 taaaatagga aggaaatgat caagtacatt cttgagcgaa tgccactcgt tggtatttgt
    19501 gtagtttatc taagcggaat tgttaccgac cataccttgc acctattacc agaagtctgg
    19561 ttcatttttt tgctaattga cttcctcatt ggaattattt tctcatcaaa agctcgatcg
    19621 gttctattaa ttgctataac gctcttctgc cttggcggaa tgaatcatgc cctgcgtacc
    19681 gttactggtc ctgatcactt aagtcaattt tatttctcta atgaaccgga cagtgtaatc
    19741 gctatggttg aacaggcaga atttagcggc gacggcaagt taaaaattcg cttgaagaat
    19801 ctgcaaataa aacactccga ctggcagaaa tggaacggca agttaattct aaccgtcaaa
    19861 gaacctgaaa acaatgtcat ttacggcgat tggatggcat tctatacccg gcttaatcaa
    19921 ccagctacgg cgcgcaatcc aggcgaattt gattaccggc gctatctggc taatcatcat
    19981 atttacgcca cggcttattt ggatgccggg cagactccga caatcattgg acgaagccga
    20041 tccctgcgca gagccgccaa ttactctcgc attaaagtcg aggagcttat tgataaatct
    20101 atgtcaggcg aacaaaatgc cattttaaaa gctttgatcg tcggagtacg cggtgaaatc
    20161 agcgatgaaa ctaatcaggc attcgttgat actggcgttg tccatgttct atcggtttcc
    20221 ggaatgcacg tagtttatgt gaccatggtg attatgctga ttctcggctt tctgcgtatt
    20281 cctaaaaaac cgaaaatggt tctagcgatt cttggcttgg gattctatgc cttgatggtt
    20341 gacctacgac cgtccgtcaa ccgggcagtt attatggcaa ttttaacatt aatcgcccaa
    20401 ggctgggaaa agcgagccaa tgtctataat actattgccg cggccgcttt gattcagtcc
    20461 ctgttcgacc cacttcagat tttcgatatg ggttttcagc tctcatttat ggcggtaatt
    20521 tcgatcgtct atgtttaccg acaattggaa tatttattgc ctctgcctct taaaacttcc
    20581 aacatcaaaa atcgccgttt gcgtgaaacc tggcaactct ttctcgtttc gctttcggct
    20641 ctattaggca ccataccgat tacggttttt tatttccaac ggattcctct catttcgctg
    20701 gtgagcaatt tagcagttgt accattggtc ggactcatag gtggtttggg tttcggccag
    20761 gtaatcctcg gatttttgtg gagcggattt aatttgactt tcggcgaagt tcagacgctt
    20821 ttaatcagct tgttgactca gattgtcaaa atctccgggc gagtaccaat tgcttatctg
    20881 acgataccgg cgataagcct tgccggggtt tattttggat atttaattct tttaattctg
    20941 ctaaacctcg ataaaaaacg agttcaaaaa gtagctttat tcggctttct tatcggcatt
    21001 aattttctaa tctggacgca aaatttatcc aaaccggttc ttcaggtaac gtttctggac
    21061 gtcggtcagg gcgacgcgat ttttttggaa ttccccaatc acgaaactat gttaattgat
    21121 actggcgatc ggactttccg acgcgattac ggagaattaa cgatcgcgcc attcctcaag
    21181 cgcaaaggct tccagcgcat tgattatctg gctttgagtc acccacataa tgaccatatc
    21241 ggcggagcgc cttatcttat gcgcaatttt caaatcggcg aaatctggga accggacata
    21301 tccgccagtt ctcagattta tcgcgaaatt cataccctcg ctgattcctt gcaaattcca
    21361 atcagacacc tctacgccgg ggattgtttt cccttagccg agtcactgac tgtcttcgtt
    21421 ctgcacccgt caaaagcgtt ccttgagtcg catcctcccg gattcaacga ttactcaaat
    21481 gttctaaaaa tcagctatcg ccagattgac ttcctcttct gtggcgatgt cgaaaagttt
    21541 tccgaaagtt atctaaatct atggcgtgcg accttgcaat cagaaatatt gaaggtcccg
    21601 catcacggca gttcgacttc ttcaagtcaa acatttctcg agcagattac tccggatttg
    21661 gcagtaatat ccgtcggaaa aaataacaaa ttcaggcatc cgtcggctgg taccattgcc
    21721 cgttatgaag cactcagcac agaaattcac cgcactgacc tgagccgcgc ccttatcatt
    21781 aattcggacg gtcataaata ctggatcaag ccatggtgaa tttatttcgg tcttaggttt
    21841 ggtttttagt acgctattcg ataaaattgt tttagtttaa ttattaaaat ttgatcacgg
    21901 agtaaccgta aatatggtga acaatcaacc ggaaaatccg gaaatcctga aaggctatac
    21961 ggcgcactgg atcgttcccg tttctcagcc gcctattgaa aagggagtca taatagttca
    22021 aggtcaacaa ataatcgagg tcgagagcgc agacaatctg caaactaata tcgaaactcc
    22081 tttaattgac ctcggagata gtgtcatatt tccgggattt atcaacgccc atactcatct
    22141 tgaacatcca cccctggccg aaacgccggt taattttcgc aaatatatgc aattcgtctg
    22201 tgattttaca cggaccgacg aaatcagcaa ggcggattat gcggccgcca atctggcaga
    22261 aaatatcaac ttcggaacgg ttgcattagc cgattttacc tccaaaggcg cttcttactc
    22321 agccttaatg gaaaatccag tctttgctcg tcaattcttc gaggtgactg gtttcaaaaa
    22381 ctaccaggct tccgagataa ttcgaacttt ccgtgagcta atccaggatc aaacccctgt
    22441 gaaaaaaatt accaaacatc tcaccccatc ttacgtttgg gcgacttcgc cccaattatt
    22501 acatgagatc gcattcaccg aacggcatat tgctatccac attgatatga tcccagaaga
    22561 aaacgagttt actctcaacg gtagcggttt attaaggcag attttactcg ccaacgagga
    22621 ttttgattat agctggcagg taccgggttt aacggctatt cgctattttt ttgctaatca
    22681 tttctacgct cggcacaata tactaaccca tatgaattgc gtcaccggcg atgaaatcga
    22741 cttcatcaag gaatttgggg taaaagtcaa tgtctgcctt tgcccacgca gtagcaaaat
    22801 cttatcgcaa atcaagacac cagttgattt actactggaa aaagggatta acctatgcct
    22861 cggcaccgaa agtcgggttt tagttcctga cttcgatatt cgtaaggaaa tgatcgaatg
    22921 tgtagatgcc tatggtgtat ctccggaact tgcccttaaa tttgcaacac tgaatggtgc
    22981 ctacgctatt ggatttcaca aggaagttgg cagtttggaa aaaggtaaaa ttgctcggtg
    23041 tctggtcgtt gattgtagcg gtctgacgac taccgacccg tatgaaatga tcttaaatac
    23101 cccccactct cctcgctggt tgatctgaaa atatattttt cttaagataa aagtaaatac
    23161 ttgttgctaa tgtcaaaaat tttccttatt aattagggtg agattaagnn nnnnnnnnnn
    23221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta aatacttgtt gctaatgtca aaaattttcc
    23341 ttattaatta gggtgagatt aagatttcta agtctcgctg aattggtcac tgaaacctac
    23401 ttaactgaag aagaaaaaat aagcgccaac gatgacaatc ggagtgcgat caattaatga
    23461 atacggcttt tcgtcacgta aaatataatt cttaaccaaa ggagtcaaca tgattagaga
    23521 gcagattcta aaagatttga ttcacctccc gcagaacaaa tttcccgtaa ccagcgctta
    23581 ctttacactc gataatattg ccggcaatcg gaagagtcat atcgttgaaa taaagaagca
    23641 aattcgctat aagaaagaaa aaacgtactt ccggcaatta agtattgagg agcaagagtc
    23701 ggtactgtcc gattttgatc gtattttaca ctggttcgga aaagaattaa atccagcgga
    23761 gagcatcagc tcaatctgtt tctcctccag tggcgcaaat ttatggcgca cactcaattt
    23821 taaaaaacct ttgccccaaa atattctcgt gatccaaccg tatccctaca ttcgacccta
    23881 tactatgctt ttcagtcgct accgcaatta tgccgtagtc ttgattgatc gttccaaagc
    23941 taaaatattt gaatcgcatc ttggagagtt cagggaacat tttcacatcg aagacaacgc
    24001 ccccgagtca gtcaaagtcg gcggctttaa aggacgtcag gaacgaaaag tcgagcgcaa
    24061 tattcatcaa ggtgtcattc aacattataa agaagtagct caagagattt tcgaccttca
    24121 tcaaaagttt gattttcatt ggataattct tggcgggaga aaagaagtcc tggcagaatt
    24181 ttcaaaatat ttgcatccat atatcagtgc gcgaatagct ggccagatcg aagtcgaacc
    24241 gaccgcaccc ttggcagaag tcctgaaaaa agtagagaaa accgaagaac aagcaagaaa
    24301 taattatgaa caagcactcc taaaaagctt actcgatcga aagtcagccg gccagactgt
    24361 ggacggtatt caggctgttc tcgataaaat ccaggacaat tgggttgata aactcatcat
    24421 ccaagaacat ttttttaaaa aaggattatt ctgccgcaat tgtggctttc ttagcttaaa
    24481 accacttgcc tcttgcccga ccggatgcgg aaaattagaa agaaccaatg atctggttga
    24541 acatctggtt caccgagctt tgaaacaggg agtggatctc cagttcgtta gtagcaatat
    24601 ggatgattat gcggggattg cggccatttt acgcttctct atcggtggtt aaatttcatg
    24661 ataaatctcc attctcagat tgaaaaaata atttagagag cttaattatt ggctatttca
    24721 attaaaattt taagtaattt tggctaactt gcttatttac atcaattttt tacttgacat
    24781 tttaccattt taaaatatat attctatctg gatgcaacgc tatagtgtca aattaaaaaa
    24841 tataggcact gtttatctga gggtgtggga ttatgaaagc caaaaatttt tatgtatcta
    24901 tcattctggc gataagcctt agtgcgtgtg gtacttctcg agctaaacca gaaaagaccg
    24961 gcgaagaaac aactaaagcc aaatcggaaa aagctgaaaa tttacccgag tcgcaaagtt
    25021 ctaaatcaac gtcttcaggt ccccagccca aaatagccac agcgccagtc aaagctaccg
    25081 ccgccgacca aagccgcctc atcaatgaac ttactttcga agtaaaaaaa ctatccgccg
    25141 aggtaaaaca tctccaatca gccctgaaag aattacaggc taaaagccaa atgtgggata
    25201 atcctctttc catttacgat aaagaaatta tcatggcaaa tggcacctcg attttcggca
    25261 aaatcattta tcaagacgaa aaaatgatta aagttgaaac ttggatcggt tatctgatta
    25321 tcgataaaaa tcaggtggtg agagttgtga ctaatatagt tgagaagcca gacgaacaat
    25381 atgtgccgcc cgaactcgcc agcgatatta aagatgataa gccaatcacc atccaagccc
    25441 cttatatatc aaaaaccggt gaaccagcgc caatcggtga accgaccagc cgtgttccta
    25501 attgtgtatt agtgggtaac atcaaagaac gcaaggatct aagcggcaat actattctct
    25561 ccggagaaat taaaaatatt ggaggtcgtc gggctgattt tgtcaaaatc aatatcgttt
    25621 ttcgtaagaa ctggagcggc gatactgaaa cccgaacagc ttttgtggaa ggctcttatg
    25681 ttacttttga aggcacggga atcacgagta ataattccct gttgccgggt gccaccggaa
    25741 ctttcgagat gatcatccct aaggatttcg gacaatttat aggttatact tacaatattg
    25801 attgggaggt ttatagataa tgccccgatc atttaacctt tcgcttttaa cggttgtgat
    25861 tacggctgta atttgcttcg gtcagggtac gagttcttcg tccggtaccc cgattcatga
    25921 ttccaataaa acgattgatc agaaagatat tgaaatttac aatctaaagg aagaacttga
    25981 atatctcaaa aaagaattga gaaaattaca ggagatcaat acctctttat gcgattcact
    26041 ggaatccaaa catactcttc ttgaaaccac atctgaaaaa aatgttgatg aaaattacaa
    26101 tctgcgcatt tatcaccaga ctatcgaaga tgaccgacgt cttaatcaag agattgttaa
    26161 agaaattcaa tccaccgccc ccatcgcagt tccatttaaa tcacctgagc cgagtggtga
    26221 tccggagttc cgagaacgat ataacgaagc cttgaatcaa tatttcgacg gtaatctaag
    26281 cgacagtcag aaaaatttta aaaaactgct ggaactgcgc caggatcatc ccttatctga
    26341 taactgccag tattggctgg gcgagtgcct ctattcccaa gaaaaatacg aagaggcaat
    26401 tgcggctttt caaacggtaa aagcattggg tgacggtaac aaagccgatg ccgctctctt
    26461 caagattggt ttatgctatc taaaattaga caagaaagcc gcagcttctt cggcttttaa
    26521 cgatctgatc aatttttatc ccaagagtga cttggtaccc aaagcttacc aatatcttct
    26581 ctctcaagat aaattctaac atgaaaaaat ttttaattat gataactcta ccaactttgc
    26641 tctctgccca ggcgttcgat tcatcccgcg cagttggtac tcctaatata atgtatgatc
    26701 tggggatatt tatagagccg gcgagggagt ctgcccccct tccaaaaggc gttgctaatc
    26761 caaatttggt tcccatccag aaccaaatag actccctcct ttcggctttc tcgataatga
    26821 ataatcagat cagatcgctc gagcaagcta atgattctct tcaaaaaaaa gttaatgaac
    26881 tctcaaagcg cattttcctt tcgccttttg cgagcttgac agtccccgct caaatctact
    26941 cacgtcacga gggtgaacgt tttttacaaa gagggcgagc cgcctatcag gcgaataatt
    27001 acccggtcgc aattgattat tttctaaaag tattagccag caacatgccc gatgcaacta
    27061 ttggcgatac ctacttctgg attggctatt gttacgttca gatgcaagat gaatatctag
    27121 ctatcgaata tctgaaaaaa gtggcggaat acccattatc agagaagcta gacgacgctc
    27181 ttttcttgac cggaattacc tatcaaaagt taggcaatcc ccaaatggct gaaatctttt
    27241 tcagccgact catcaaaaga tatcctgaag gcaacctcgc cagattagct gccttagaat
    27301 taaaacggct aaagggtcct gattgaacac taaacggagc ttcgctctat gaccagacct
    27361 catctaaaaa tttcactact tttactgcta accataacat tcttacaagg tgagtcggta
    27421 acacgtcttt ctttttccca tcctggatat atgatgagaa ttccggttag tagtgtttat
    27481 cgaacaccgt acgtatttcg taccggtttc gccagcgata cctatggatt ctcggataca
    27541 ttaattgcca aaggaatatt ctttgaagcc gacctcacca ataccttcaa ggtcggactt
    27601 actactatcc agggcatttc cggcacccgt ccggtggaat ttggtcttca ttttcagaaa
    27661 cgtgttttta cctatggcga tattagtttt acggctggtg tgcatgacat ggttctccgc
    27721 cagggtgaaa aaaacctcaa tattgatact gaaacacttt caattttcgg cctgctgagc
    27781 aatgaaaaac agtttactaa taatattctc catacctata tgggatttgg tacaggcggt
    27841 ttggcgtcca cgtttggaac tgattcgact gccaatatgg gtgttttcgc cggaatactt
    27901 gttcatacaa atatcagaaa agatcaaggt ggtgtgtctt ttatggtgga atttgatggc
    27961 ggtggagtta atcttggcac acgcattccg ctaacccaag attataacct aagtatcggc
    28021 atcaatgatg ttactcatct ctataatttc ggcagtaaaa attatagcac cacacctcaa
    28081 aatgagcatc ccagccttag catcagcctc gattttacca taccgcgaat caaaccggaa
    28141 aatataaagc ggcgtgctct cgaaggaatg gttgaccctc acacaaaggc aattctcgat
    28201 gttgaggaac aattcgccgc caagctggat tcgaccctta aagccgccga cttcgaaatc
    28261 gccaaattac gagattcact tcgggtatat gaaacccaaa tcaaatatct caccagtcaa
    28321 gtgacccagt tgaaacaaaa aagtgccgtt ctggaggatt cggttcgttc gattaagctc
    28381 gccaaatatg ctatggaacg gaatattaat ctagctttaa agcacttatc aaattctctc
    28441 cgctattttt acgaaggcga ttatcagcaa gccttaaagg aagttgatgc cgccatcgaa
    28501 ttgaatccca atctagctct ggcttatgcc agacgtggtt cgatctacta taaaatgggc
    28561 gatatcgagc gggcttcaat taactggaat ctcgctctta aaattgatcc ggaatacgat
    28621 gacgtccgta atatattacg cgctatgagt gaaaatagat tacgtagtgt cggtgaagaa
    28681 caaaaggagt aaaaactagt atggatattg ccacgatatt tggaattatt gctggaacag
    28741 tattaatcat aatgggtatt tccggattga ccagtgactt caaaatattc tgggatattt
    28801 ccagtgtttt tatcgtagta ggtggctcct ttgcctcgac aatgatcagc ttttctgtaa
    28861 aggaggtctt tagtttaatc ggttcaatta tcgctgtttt taaaaaagaa aaaatcaaga
    28921 tgactgatga agttgagacc attgtggaat tagctactgt ggctcgcaaa agtgtacaag
    28981 atttagaaaa agcactcgtt gatgttcaaa atccgttttt acgcgatggt ctccaaatgg
    29041 tagtggacgg ctataatccc caggaaatcc gtgatattct ggaaaccaga atcgaatatc
    29101 ggactttacg ggagcgaggc gaagccaacg tcttgcgcac gatgggaaaa tattcaccgg
    29161 cttttggaat gatcggaact ctgattgggt tggtagttat gctctatgga atggctcaga
    29221 ttagttccgc tggcttagat ccgatgaaag tgctcggcga aggcatgggc gccgctatta
    29281 ttactacttt ttatggtgta atattagcca acttaatttt taatcctatg gcagtcaaat
    29341 tcgagactcg tattcagaaa caaaacatcc tacaaatgat gctaattgaa ggaatactat
    29401 tactgcaggc tcgcaaacac ccgcttatca ttcgtgaaaa actcaattct tacttacgac
    29461 cgcgtgattg gaaaaaccct gaagagatta aatagtgatg gctgacgagc agaatccaag
    29521 gcacaaaata ttttctcacc aggacactgg cgatgaagag ccgtggctga taacctattc
    29581 agatatgatg tcattactac tgactttttt catcttaatg ttttctattt ccactattga
    29641 cccggtcaaa ctggaacaga ttggcaattc agttggtcaa gccctcggag gcgccaaacc
    29701 taaacccacc gagcattttt caattaaaga aatctatgac aaggtcgttg atgtcatcga
    29761 acaggaacaa ttaaaaggag tcattgaggt ggaaaccagt tctcgcggcg tttcaatcaa
    29821 aatccccagt gaaatttcct tcgcctccgg caaagccgat ttggatccac gcattttccc
    29881 aattctgaat aagttagctc ccatgattgc gcagtctaaa tttcccatcg ctatcgaagg
    29941 acatactgac aatgttccca ttaaatccga cgtttttcca tctaattggg agttatcatc
    30001 cgccagagcc gcgcaggtgg ttcgctactt cgttgggtta ggactgccgt ccgatcgttt
    30061 tcgggcaatt ggatatgccg atacccgtcc gcgcgcaagc ggcaaaacaa ttgaagaagc
    30121 caaccaaacc gaagagcaaa gagctcaaaa tcgtcgggtc gaaataattt tcttaaccat
    30181 tggttaaaaa gaggattaca ttatgtcaca ttttaaagta aaacagttga ttttaatttt
    30241 tagcctcggc ttatttactt tattaattgc ccaggaaaat ttatcatatc tctctcagaa
    30301 aatgtcactc gaaaatagca tcaaagaccg cataaccgaa gccttagaaa aattactcga
    30361 tgatgtcaaa ttcgtggtca atgtcaaagc cgaaattgat ttcgcaccca ttgaacaggc
    30421 tgagccgacc gaaacgactg aaaccccacc tgtaggcact actccccagg aatcacgccc
    30481 gagagctact gaatacgttc tacctcttcc gggctttgat attccaactt ctgtaattca
    30541 atcccaggaa cgaatacagt ctcaaacgca gctcgatgcc ttaccacaac cagctacagg
    30601 tgcggctaaa tcgacttcta ccaccgaaaa aaaagtcgct acatccgttc cggttataaa
    30661 acgtcaggaa atcaatgtca ttctcgaaga tggtatctct cctgaaatta tagaaaacgt
    30721 cagacaggtc gtggcggtcg ccgctcatta tgatcgggcg cgaggcgatg tcatcagtat
    30781 tatgaccgcc aatttcaaaa aaagccccga taaagatgcg accgaaacca ttatcctcaa
    30841 aaacatagcc gaaaaaattg acgacatcga acgtcgtcaa acagccgccg aatataatgc
    30901 tcgcttcgaa cagcaaaagc aactcgaacg ccaagccatt ttgcgagatt cattaaaaat
    30961 tgatgaactc aaaaagcaaa tagctgagct acaggcacaa ttacaaactc cccagataac
    31021 cgaagaccag cgtcaggtta ccgaacaaca aactactacc cgcgaagccg aactggaaag
    31081 cctgcgcgag caattacgag aaagcaatcg ccgcctgcaa gaactggaat taagttctct
    31141 cgaaaccacc ccacccagta tgttaagagt tcgcaattat ggtatgtggt acgcgattat
    31201 cgcggcgata gtgctactga taattctcct aatcgtctta ttagtcaatc atcgcaagca
    31261 ggtcaaaaga caggaactgg agtggggtta tggttcccaa atcccaatgg gaccgcgtcc
    31321 gactcagcca attttaaccc gccagccaga ggaaactccc aagccccaat cgccagtacc
    31381 cccgattact caacccatcg caacacctgc tccaccagta gaatcagctc aacccgccca
    31441 accctctgca ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttgactt
    31561 tgaagcccag aaagaagaaa taaaaagtat gcggcaatcg gtaatttcaa tgtcagttgg
    31621 tcaacctgaa acagctttac gaatcatcaa tgcctggata agtcaggata tacagccagg
    31681 caaatccgaa actgagagtt aaaggagtaa gaaaatgata gacgccaaaa acttgagtgg
    31741 actggacaaa gccactattc tctttaaagc ccttggtccc gacttatcag tccagctttt
    31801 caaaggacta agtcgtagcc aaatgcaaaa aatccgtgaa aatatggcca aaattaccaa
    31861 tattccattc gatgtcaaaa aagccgtcct cgaggaattc tacttcagct tcgtttcgga
    31921 aaaatttact cccgccgagg aggagaccaa aaagccattt gagtttatgt tgaaactgac
    31981 cgacgaacaa attgcctatt tgattgccag cgagccacca atcattatgg ctctggcaat
    32041 cgcccagcta ccggtcgaac gccaggtcaa cgttttacaa aaactcgatc ccaccgtcca
    32101 acctaaagta ttaactgaaa tgggacatct cacggaaatt ccgctggaag gcgtggtcag
    32161 tatcgccaat aatcttaagg acaaatcggc ttttattcca cgagcttccg aatttaaacg
    32221 cggcggcggc caagacgtag ctggcatttt aagtaaaata ccgccccgtg atgaaaaacg
    32281 cttcctcgag tttttatcta aagaagcacc cgaactggtt gatcagataa aacgctatta
    32341 cttcagtttc gaagaccttc ccaaattacc accaaacatc ctggctgaag tgctaaaatc
    32401 ggtcgaaatt tccgaaatag cttatgcttt aaaaggtcaa ccggatgaaa tacgcgacct
    32461 ctttaaaagc ttactacccc aacgttctca gatcattctg gaagatgaga tgaagctatt
    32521 agaaggacca caaccacggc gtaaagtcga agccgctcag aaaaaaattg tcgaaaaatt
    32581 acgcgaactc gaaaaagaag gacgcttcaa tctcgaagat tttatggata ccgactttat
    32641 tgaataatag ttttaataaa taccaaaaaa gccccgcgct ggggcttttt taatgctaat
    32701 ttgatagtct ataccatcta ttttaaaaca actaccttat gaaaattatt aagaccttat
    32761 taatcaccct gctactcggt ggttttgcca acctgaaatc tgacactttt gaacaacagg
    32821 aagagagagt tcaaattgct cttaaagcaa tgtacaatcg ggaatacaaa cgcgccgcca
    32881 gtcttttcca cgaagtatta aattccactc cctttcatcc tatgggtcca ctgggagcat
    32941 tagcggtcga atattatcaa aacgaaggat taatcggcta tcaccagcgt aatcaacaat
    33001 ttcttgtgga tattgagcag gctttgcaaa tttatgcgga ccagattaaa caaaacccat
    33061 cgcattcgga atacttcttt ttctatggaa ctgttatggg cttgcgcgcc cgcattcaat
    33121 tggcagaaaa aaattacttc ggcgtgctaa ctagcggtta taatgctatc cgatttatca
    33181 aaaagaccga aaaactctat cctgaccaac cggatttcca attgccggtc ggtctattta
    33241 actattatgt cagcatctca gccccttata tgaaaattgc cagtcgccta atgcgcgaat
    33301 ctggctcgcg cagtgaaggc ctccaagcca tcaaagccgn nnnnnnnnnn nnnnnnnnnn
    33361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33421 nnnnnnnnnn nnnnnnnnnt ctatggcagt ttggcgcttg cccaaggtcg gctcgatgaa
    33481 gccgaatcga atttgatcga attcatcaaa tcctacgatt atgaactgga ttatgacctc
    33541 ggcaatgctc tcgtgagatt aggtaatgtt tacgatctac aaaaacgccg atccgaggct
    33601 atcgcctgtt atcgcaaagc cgtccagctt aataatcgca cggcggctgt cagcctggct
    33661 aaccaatatc tgaaacagcc atataataga aactaaaccc ggtttcctaa atatattatc
    33721 atttcacaga tcaattttgg gtttgatttt taactgattc gtaatatgat aactccgatt
    33781 ttttctttat attcattccg gctttttaat ttctaaaaca atgtcaactc ctccttgcca
    33841 aacgcgctac attatgaaaa taatcaaaat gaggttaaga acttatgaac ggtcaaattc
    33901 ccgtgcttat ggttaacggc gaatcgctcg ccgccgcttg ggaaaaatca ttagtcgaac
    33961 tataccaaaa aggttgcgat atcaaaaccg agtacgacaa accttctgat ccgcctagca
    34021 aagattgcac tatgatgatc gtcgtccaaa accctttagc cgaaccaatg attcattgtg
    34081 attttccagg tggattgaca gacctgcaag aatatgttct ggaagtgttg gaaggaatca
    34141 aagaccattg catccggtcg gctgagaatc ccgccgatgc ccgctgggaa tatacttatc
    34201 accaacgttt attcaattat caggtgcctg gtttcgcgaa agtttatgac cagattgcga
    34261 ttatggtcgc aaaattaatc aaagtccctc atacgcgtcg agcccaagca atcacctgga
    34321 aagtatggga agacaacgat tgctacgacc cgccttgttt gcaaagcatt tggtgccgtt
    34381 tgctgaaaga cgataattcg atctggactc tcaatacgaa tgtccgcttt cgctctaacg
    34441 atgcttacaa agccgccttt atgaatatgt ttgcgctaac ccagctccag gtcgaaatcg
    34501 ctaatcgaat cgagcaatca ctcggcgaaa aaatcaacat cggtcgctat gtccatcaag
    34561 cggatagcta tcacctctac ggttcttatt ttgacgaatt ccaaaatcgt ttcctgaaag
    34621 ccctcgagac gcgctctttt gaggagcgga ctttccgtta tgccgacgta aaaagcatta
    34681 tgacagaagc catcccccag attcgcgcaa aagccgctcg tatgggacgt gaataaaact
    34741 aaaccgttta caaatcacat tagactatcg caacctttgc gacattctaa gtccaaatcc
    34801 caattaattg gatgattctc tattttgcaa tcatataact tttataaagt tatgaatgca
    34861 acaaatttat agatgaacta acttacgata acttgttaat ttctataagg ttttttaatt
    34921 gttgtagtct tgaagatttt agcctaaggc tacatctaaa atcatcataa tggtaaaacc
    34981 cagcatcgct cccagagtcg cgacatcggt actaacgtct ttctgagatt cgggaattaa
    35041 ttcttcgata acgacataaa tcatcgcccc cgccgcaaac gacagggcat agggcaacaa
    35101 agcctgaatc gtcaaaacca gataagcacc gataactccg gaaatgactt ccacaaaacc
    35161 ggaaagttga ccgtaaaaaa aactcttgcc gatcgaaagt ccctcccgac gcagtggaac
    35221 cgtaaccgcc acaccttctg ggaaattttg aatgccaatg cccagagcca gggcaactgc
    35281 accagctagt gaagccgaag gcaagccggc tcccgccgca ccaaaagcca ctcctactgc
    35341 caaaccctcc ggaacgttgt ggatcgtgac cgccagcact aaaagcaccg tcctttgcca
    35401 gttagtttta ataccttccg cctgatcaat cggcaaacct aaatgcaaat ggggaagcag
    35461 acgatcaacc aatttcaaaa aagcgccacc taataaaaat ccgaccgccg ccggtatcca
    35521 agccggacta cccgatgcgc ccgccatttc tattgccgga gccaaaagcg accaaaagct
    35581 ggcggcaatc attactccgg cggcaaaacc cagtgcggca tctaataggc gttgactgac
    35641 ttccttgaaa aaaatcacta atccagcgcc gccagccgtc accaaccaag taaaacaggt
    35701 tgccaaaaat gcttgaaaaa cgggacttaa ttcaccaaac cattgcataa atatcctcat
    35761 taaggcgatt acttacggag cctcttccag gcattaatca atccattagt tgagctatcg
    35821 tggctggtaa tcggct
//
LOCUS       DAOMJU010000012        34801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1048_length_34801_cov_16.458988, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000012 DAOMJU010000000
VERSION     DAOMJU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 34801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34801
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1048_length_34801_cov_16.458988"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2932)
                     /locus_tag="PLO58_02220"
     CDS             complement(<1..2932)
                     /locus_tag="PLO58_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73637.1"
                     /translation="MKHSIKYLTIEILSILLMTIVTSQIIFANQSGKAYRRSSIHNGN
                     LVRTVFGNWGVIGQPREKGPRGAWLFDTNGYIGDVSPMVGAEVNYHSEELDSTIIFHS
                     VVVCPVDRPATAREESPTGDQWTFEPVSGYFNESSQGIAMSTDRYSWPPYWPDKMDDP
                     EDPGWPEQWNGFFGKGVTNADQESYFVMDDNNDVEFNSGHNLKGVYFKPDNRDANRNG
                     LGLEVKVRGMQWAQFLAQDVIFWLYEITNTSTTDYDKVVFGMIAGTYNGVTGTDDSPQ
                     EYDDDYSFFDVERDLTYTGDYPNNNSRNPKWQGPVGLVGYAFLESPGNPYDGIDNDGD
                     NKPEEHPGKNLIFTAPLFTESDFDSVVYNKGDQIVLIDKDYKRTFHTITSDTETVVTK
                     GMQITIIAGQTKLVEGNVLANGSINPNVYDGIDNDLDGLIDENYLLHYRQRRVDQNGV
                     VLFDTLNPVAYKDYRRGLGIDDPLIDEARNDGLDNDGDWNPETDDVGSDGKPGTNDYG
                     ENDGKPTLGEPNFDKTDVDESDQIGLTSFNYFVPSNAYPMADDELLWEWLKPGYFDVP
                     SSIQNGKPVAGEDGDFIYGSGYFPLRAGDTQRFSLALVYGYDLTDLLKNRETVQDIYN
                     SDYRFPQPPDKPTVTAVAGDGKVTLYWDRKAEESIDPVTKEKDFEGYKIYKATDPDFN
                     DVFVITDANGVLTGYKPLAQFDKVNGIKGIFEASSDLYQSAGGYSYYLGSDNGLVHSY
                     VDEDVQNGRIYYYAVVAYDHGDASNDIFPSENTKFISVQSDGTVITDINTVQVRPTAK
                     VAGYDEQNSELALKHLEGPATGVIQYKIIDETLLNGHTYKVNFTDTRTDGIDNDDDWN
                     YLTCDVGSDGKPGTNDYGEGDGIPTSGEPNFEWNDEDEFAQITSTYSVLDKTGVEEFI
                     NLDTSYVWLTHQNIQYDDILIASESDPNNYLSLSKFEIDTLRGKIKIKADSNLKSGSY
                     RIFYK"
     gene            complement(2929..5727)
                     /locus_tag="PLO58_02225"
     CDS             complement(2929..5727)
                     /locus_tag="PLO58_02225"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO73638.1"
                     /translation="MRYKDWLITVVLILTAGYLFGGNTGKITGIVTDSQTGLPLIGCN
                     IMLEDTYLGAASDTDGRYMILNVPPGEYRLKAQMIGYQPKVITGVIVSADLTTTINIG
                     LSTQVLEGEEVIVTAERKMIVKDMTATASNVKAEELAALPVTEVSDVLEMQAGYVDGS
                     LRGGRRGEVAYLIDGIPVTDSYDRSAVIDVNKNMIQELQVISGAFNAEYGKVMSGIVN
                     ITTKQGSNKHGASLEMYIGDHLSTHDEIFDNIGSINPLNIHNAEISFFGPLIKDKLFY
                     NFNTRYIYFGGWLHGQNRYKPTSILAVSPDGSHFLLDSTTMLGDQKWVNMNWNEKLYF
                     QGQLIYRLSNRSSIYYNFFYDSKDYQDYDRMFKYNPAGMLKKNLTAETHLLKFQQQLS
                     SKTFFTLAGSYYYRKYTGRLSADTSYNDSTTYVHPAYLYQYPYQFYFGGTQNDHEYRD
                     SRTYLAKWDLTSQINGQHQIKGGLEFQQHDISLRTFNIRPATGETYIDFASGLNIPST
                     WNPYIHPTILDNSTIYASYYRHKPYEFAGYLQDKMEFSELIINIGVRFDYFEPDGVVL
                     ADPSDPEIYEPIKPENRYHDLNGNGVQDPGEPEVTLEERRQYWYKKATPKYQFSPRIG
                     LSFPISDRGVFHFSYGHFFQIPNFNYLYMNPDFDLGSGTGNQGVIGNADLKPEQTIVG
                     EIGLQQQLSDNFSVDITAYLKDIRDLTGTRAEEIIVYGGAASYSRLVNSDFGVVKGIT
                     IALNQQNPSGLYTSFDYTLQIAKGTASDPEAYRNAIAGGNQPEIQLNPLDWDQRHTIN
                     GTVGYNHKNYGINFIGRWGSGLPYTPQSSEDITSLLTNAGTKPPTLTIDARSYYRFTL
                     SGRYSVELFIRIQNLFDKLNEVNVYDETGRAGFTTDEARVEALNIKTPINSVHEWFND
                     PTYYSEPRRVEIGMRISI"
     gene            complement(5773..7620)
                     /locus_tag="PLO58_02230"
     CDS             complement(5773..7620)
                     /locus_tag="PLO58_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HQO73639.1"
                     /translation="MKNATENQVPKGKFSLARGLLVVGIVAGICLSDNLSDRTYWTYD
                     AIWYQIFPERFRNGDPTNDPTIETLEGTWPYEPQTQWSIMPWTADWYKLQPWEKSNQR
                     DFYYNAQLRRYGGDIEGIIDKLDYLVDLGINAIYLNPVFESASSHKYGATMYHHIDNN
                     FGPDPAGDIAIWQTENPNDPSTWLWTSADKLFLKLIQEVHTRQMKIIIDGVFNHVGIP
                     FWAFQDVKKNGEASPYADWFVIKSFDDPSTIEDEFEYQGWNGIPDLPEIWEDENGPVQ
                     GFRAHIKAVVERWGDPNGDGDPSDGIDGWRLDVAEKVSKNFWRDFRKWVKSINPEAYL
                     TGEIWWEDFMNNKMFDASPWLQGDIFDGVMNYRFGDAMLKAFIDREMRIKPSELDQLL
                     GDIREKYPLPAQYQLQNIIGSHDNERFASMLLNPDRWIDHGGNLVYNKDFKINRPSDE
                     LRQIQKTILVFQFTYIGAPYIYYGDEVGMWGADDPDNRKPMVWDDMQYEPETTHPFGI
                     KRPVDSVKVDTDLFNFYKSVINLRKNHECLRRGRYKTILTDDKKGIFGFERKYGTETI
                     YIFANLNDKPIKMSEINFDVTKCEKIFTVNTDTNYLGSKGIVVYKIIEG"
     gene            8499..9266
                     /locus_tag="PLO58_02235"
     CDS             8499..9266
                     /locus_tag="PLO58_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQO73640.1"
                     /translation="MRKILAVDDDPNIIELITSYFSSRGFEVYATTEGAQVLDLAISK
                     EPDLIILDINLPDIDGLSVLKTLKNRPITAFIPVIILTAKTSADFQVDGLVTGADDYV
                     TKPFDLNVLYARVLNSLRRSLLPTRFKHDQFNLLSYLLREYSKRGYKVYSKLLDNYSP
                     HPPYWRGYIPDMIIEKPEKLRCFNFETTQSIMEESFLDRLNSLVECHKNTTSHFEATV
                     IVRTMDNYKIVQKLVNEHCLPIKIKYFKKHIRRNLSV"
     gene            complement(9263..9634)
                     /locus_tag="PLO58_02240"
     CDS             complement(9263..9634)
                     /locus_tag="PLO58_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQO73641.1"
                     /translation="MPIKPKILIVDDEAVIREIYGDFLEQNGFEVATALNVETALESI
                     KAKKPDLILLDIVMPFRNGFDLYDQVIVDYPGIKFVFMTGYDDDIKITDRLSKSGKKW
                     FTKPIKLEEMLEIIRNELSAS"
     gene            complement(9635..11008)
                     /locus_tag="PLO58_02245"
     CDS             complement(9635..11008)
                     /locus_tag="PLO58_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO73642.1"
                     /translation="MEDNKPVQNPLREIVDPFVNLIKAPRALWGINLSYLLEGITYFG
                     ILSLLAMYFNDLGLNDIQAGQMVGIQTAGITLAMLFLGATVDWIGVRKALLISLSTML
                     VGRVLLTLAPQTGAPGLWQKAHIVAMLGILGVVLGYGIYQPACYAAVKQFTDKKTSAM
                     GYAMLYAIMNLGGFLPGLISPPIRKNHGIVGVYWVYVALTVVGILVVAFILSKKTVEM
                     AIANAREGQDEPVSDPETTETQSTKEKIVYYLKNFPIKDGRFMFFIFILIPVQTLFAH
                     NWLTLPMYFERAFSGVVSNNFEFFVNFNPILIFILAPMVAALTAQKDTYKMMILGTTI
                     MALPTFLLAIGPNIYSVFGYLIIMTIGEAIWQPRFLQWVAEIAPKNMTGIYMGIGQFP
                     WFLTKVITSLYSGWFLTKYCPAGVTPEQTNTETMWLIYGFIAIISPIALLIARKWMGK
                     GLEKQKA"
     gene            complement(11227..>11751)
                     /locus_tag="PLO58_02250"
     CDS             complement(11227..>11751)
                     /locus_tag="PLO58_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQO73643.1"
                     /translation="QLGAKNHQTKIGMGAVAKGYGIDQAVAVIAKHNVAGALVNAGGD
                     LRALRRVDGKKWRIGIQDPRQADNILLVIEFDSGAVATSGDYEKFFMYEGKRIHHILN
                     PRTGLPADSCQSTTVIAATAEQADAMATGLFVLGALAGQKALENFPNCEVLWICADGQ
                     KVQSAGFEKYLAQH"
     assembly_gap    11752..11851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11852..12084)
                     /locus_tag="PLO58_02255"
     CDS             complement(<11852..12084)
                     /locus_tag="PLO58_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73644.1"
                     /translation="MLNFKIPIPYFLFCVFLLTSCQPKSKLIKAQSTRFAMGTVIEIT
                     VLDTTEAEANNALEAGFAEINRISAQFWEGNPEG"
     gene            complement(12101..12574)
                     /locus_tag="PLO58_02260"
     CDS             complement(12101..12574)
                     /locus_tag="PLO58_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HQO73645.1"
                     /translation="MNTNDQYFKTDDETATVIDADQHWVTVELVEKDTCHSCGAKMIC
                     RPNSTGKRILKIPNTINAKVGDTVLIEQIGANQLKLTIVQYGLPLFGFLFGVLIANRF
                     IKHSIFGIPADIVQMFCGIIVVILTGFIIYFWSKRKAKSGFNVFRLRQTLNGKFE"
     gene            complement(12564..13382)
                     /locus_tag="PLO58_02265"
     CDS             complement(12564..13382)
                     /locus_tag="PLO58_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HQO73646.1"
                     /translation="MNSTTFLYSLLSMGGIGLFFSILIAVANKKLHVEEDPRISLIAE
                     CLPGANCGSCGYAGCNNFAEALAGGKSQISNCPVCSPSAREEIARILGVNVEIGERQV
                     AVVFCQGGDDVATKKAEYRGIKTCSAANLVSGGERACSYGCIGLGDCVTVCPFDAIHM
                     NENNLPVVDRAKCTGCGNCVTACPRGIIELHPISHHVFVLCRNRDIGRIARTVCKRAC
                     TGCLLCEKAVNGQGFKVVDNLAVVDHDVYTKELVLPTEKCPTKAIVILGTKDEH"
     gene            complement(13401..13973)
                     /gene="rsxA"
                     /locus_tag="PLO58_02270"
     CDS             complement(13401..13973)
                     /gene="rsxA"
                     /locus_tag="PLO58_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HQO73647.1"
                     /translation="MDLILIFISAAVINNYVLVSFLGICPFIGVSKKLGSAVSMGMAT
                     TFVMTITAAVTWLLNKYILVGLHLEFLQIVSFILVIASLVQLVEMFIKKVSRPLYDAL
                     GIFLPLITTNCAILGLALFLVMKQYNFVESIVFGFASGAGFTLALVIMAGIREELELA
                     DVPEPFKGAPITLIVAGCLALAFMGFSGLI"
     gene            complement(13976..14584)
                     /gene="rsxE"
                     /locus_tag="PLO58_02275"
     CDS             complement(13976..14584)
                     /gene="rsxE"
                     /locus_tag="PLO58_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HQO73648.1"
                     /translation="MAKSKISVTQEFLKGLWNENPILRMVLGTCPTLAVTTSAINGVT
                     MGLAVIFTLVMSSMMISLLRKLIPNEVRIATFIIIIATFVTVADRVLAAFYPEMSKAL
                     GPFVPLIVVNCIVFARQEAFASKNPVGRSVIDALGMSVGFTLVLLIMGSFRELIGNGT
                     IFSHQIMPLSFQPWMAIALQPGAFLTLGFIIGFTNWLNTKRK"
     gene            complement(14586..15176)
                     /locus_tag="PLO58_02280"
     CDS             complement(14586..15176)
                     /locus_tag="PLO58_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HQO73649.1"
                     /translation="MNKSLKLVVVLTLTALLSGLILAVLNTYTAPRIELNENKTLLEA
                     IEYVIPGQKTCDVKVIKDVTFYVGKDDKGQVIGVAFVAEGDGFQSRLRILVGMDPEMT
                     KIVQMKILSQAETPGLGTKIENDPTNKTDAFWFLKQFADLPAVSPISVVKGQAADKSK
                     SQIQAITGATISSRAVVDIINAAIEKNRALYLEKVK"
     gene            complement(15173..>15522)
                     /locus_tag="PLO58_02285"
     CDS             complement(15173..>15522)
                     /locus_tag="PLO58_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HQO73650.1"
                     /translation="HISNWRIPVSYLGTVVVFSGILWLINPAQYPDPIFHLFSGGLML
                     GAFFMATDMVTSPITNLGTWIFGIGAGILVVLIRQFGGLPEGVMYSILLMNGFVPLIN
                     RYTRPKYFGEVKS"
     assembly_gap    15523..15622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15623..16062)
                     /locus_tag="PLO58_02290"
     CDS             complement(<15623..16062)
                     /locus_tag="PLO58_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HQO73651.1"
                     /translation="MAEQQNIVKDGAGKEPKVKPTPNPVDKSVEKRLFILSSSPHIKA
                     STDVSSIMWLVVATLLPTVIFSIYNFGWRCLTVIATSMITAMIAEELFNLIRRRKMTI
                     YDGSAALTGLLLALCLPPGLPLSMVIIGAAFSIIIGKQIFGGLG"
     gene            complement(16106..17455)
                     /gene="rsxC"
                     /locus_tag="PLO58_02295"
     CDS             complement(16106..17455)
                     /gene="rsxC"
                     /locus_tag="PLO58_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HQO73652.1"
                     /translation="MKLKTFKKGVHPAEFKSLSNYKSIEKPPLPTEVFIPLQQHVGAA
                     CVPVVEKGMEVKTGQLIGKSGGFISSNIHASISGKVKTIEKLPHPLGNNSLMINIIGD
                     GHDEWVELPDSISDWENLTPEQINKIILDAGIVGLGGAAFPTQVKLNPPKNKTIDTFI
                     LNGCECEPYLTADHRIMVEHSEEVVLGLRIMMKALGVSRAIIGIEANKPDAIAALTKA
                     TEKFWGIIVQPLKVKYPQGAEKMLIDAVLNRKVPAGGLPMDVGVVVNNVGTALAVAEA
                     VTKRKPLIERVVTVTGQGVREPKNILARIGTPFQNLIDFCGGLTDDTVKVVMGGPMMG
                     VAQASLAVPVIKATSGIVCLTDKIAVERKEYPCIKCGACLHVCPMNLIPTRLARLSQF
                     AKWDEAEQYGIANCIECGSCAFICPASIPLVHHIRTGKTRVAEIQRQKRTEKKAVSV"
     gene            17614..17967
                     /gene="ssrA"
                     /locus_tag="PLO58_02300"
     tmRNA           17614..17967
                     /gene="ssrA"
                     /locus_tag="PLO58_02300"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(17946..>18500)
                     /locus_tag="PLO58_02305"
     CDS             complement(17946..>18500)
                     /locus_tag="PLO58_02305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO73653.1"
                     /translation="ERSIKVIAALRNTWPNLVGIITGMPKRENGALTEYAASLYNLVQ
                     ENNLSNNLFFLGDLFERLNVPAEKNRAVVRDLYFISNLVLYLSMDEGFGLPILEAGIA
                     RTPLALSQIPVFREVAKEGALYLPLDESPEYNANRIVKYLTDNQPRSDVLYKSIIQKN
                     NWDDLWESHLNSIFNESMSGGGGN"
     assembly_gap    18501..18600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18674..19609)
                     /locus_tag="PLO58_02310"
     CDS             complement(18674..19609)
                     /locus_tag="PLO58_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73654.1"
                     /translation="MFIKYYPNKYERHQLIEDLKIIRDKGYDGVFYCINKPYETGDLT
                     IKYFPVARMIELTKLAGLKAGIILDCFQNPFLWQMDIFSPPVNYLGIAYTPRNKYYPV
                     CPNNPIGHDFYHLLLQKTYRLGQIDYCMFENLRFPFFWQEENLDIQHHVPPYCYCPFC
                     VTEFSSMVGEVVSFTSQIVEMLPDWLEWRSGIIANLVFDACDALIKKTKIIISTPPLA
                     LIDLPFATGQLPMAYVDNGCLISPALYHPSKMGNILWVEDILDQYRIEFKMTKLFPTF
                     KVQSESDLKQYLAIDSTENFAGLLFDNWQSLKYEN"
     gene            complement(19663..19738)
                     /locus_tag="PLO58_02315"
     tRNA            complement(19663..19738)
                     /locus_tag="PLO58_02315"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19700..19702),aa:Pro,seq:ggg)
     gene            complement(19798..20184)
                     /locus_tag="PLO58_02320"
     CDS             complement(19798..20184)
                     /locus_tag="PLO58_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQO73655.1"
                     /translation="MEKPIKKLYYSIGEVSEITGLKQYVLRYWEKEFPSLTPTKNRAG
                     NRAYREKDIQLIQYIKHLLYDKRFTIEGAKQKLKEMKPEDWQKAIDTENRNELDETVG
                     ERTDTAMVLSEIKKGLNELLEMLNNW"
     assembly_gap    20295..20394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20395..20615)
                     /locus_tag="PLO58_02325"
     CDS             complement(<20395..20615)
                     /locus_tag="PLO58_02325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQO73656.1"
                     /translation="MLNRGYEFATLSNLQANKNKPNLIALTFDDGYQDIYLYAFPILK
                     SLGIPATIFIITDYLDRPNTWDINLGGIH"
     gene            complement(20748..22001)
                     /locus_tag="PLO58_02330"
     CDS             complement(20748..22001)
                     /locus_tag="PLO58_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQO73657.1"
                     /translation="MNQKIIPARRTEKVTYAIRDIAVKAKHLEDQGRKIIYLNIGDPP
                     VYDFETPFELRQIVSEKILQSSANSQLNVSVYGDSMGLIEAREAVARYATEKKGIKGV
                     TANDVFMANGASEAITLAIAALINPGDNIMTPSPGYPLYSGQIPVYHGNLNPYLLNES
                     TGWQIDFDELEKRVNKRTKAIVVINPNNPTGANYNRESLLNVINFARRHNLVIFADEI
                     YDKLLLDGQTHTSIASLADDVPVVTFGGLSKNYIMPGWRVGWAIFSDPTAVMADYREA
                     INKLLRARLCAVHPQQYMVAPALNGNDSHLPQVIQKLTIRRDITDKMLNEIPGISCVK
                     PEAAFYAFPRIQLPPGVTDEQFVLELLNDTGVLVVYGSGFGQQRGTNHFRVVFLPDEN
                     ILRHSYTLIGEFTAKFYRKYNFKPE"
     gene            complement(22041..23282)
                     /locus_tag="PLO58_02335"
     CDS             complement(22041..23282)
                     /locus_tag="PLO58_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQO73658.1"
                     /translation="MISENIQRTILPNGIRVLSEYVDSVYSVSLGFWIQAGSQDEEDN
                     ENGIAHLLEHMVFKGTHKRSAFAIAHEIESLGGIINAFTSRNVTCFNVQLLHENLAKG
                     VEVLSDLILHGRLDPHELEKEKRVICEEIREIEDTPHDLIHDLFIQQLFPNQAMGRPI
                     QGTVESVNALQVSDLKRFLKRHYTTGRLVVSAAGRVNHEQLVKLVEKYCQDMGNGADY
                     QEKDSCNTQPETQKIYPKPIMQSHVILGRRIFGQGDSRRFQLGLLNMILSGGMTSRLF
                     HNIREKYGYVYEVYSFSDLFLKDGLFGVYAGSDKNRLDMVIDKINNEMTALYRKPIPV
                     REYKKAKQQVKGGLVLAMENMNARMSNLAKTEIYEKHIITIAELLEIIDKISIEDLQE
                     LARYLFDRNAFIETIIQPIES"
     gene            complement(23286..25481)
                     /gene="pnp"
                     /locus_tag="PLO58_02340"
     CDS             complement(23286..25481)
                     /gene="pnp"
                     /locus_tag="PLO58_02340"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQO73659.1"
                     /translation="MYQKKMTLGGRELSIETGRMAKQASGAVVVRYADTMILVTAVAN
                     FDAVTEQDFFPLQVEYRERAYAAGKIPGGFFKREGRPSEKEILGSRLIDRPIRPLFPE
                     DFHCDTQIIANVISSDQENPADVLGIIGASTALMISEIPFSGPIAGVRVGRVNGEFIL
                     NPTIKQMEEGELDIVVAGTEDSVVMVEGECKIISEDLLIEAIEFGHSAVRELIQFQKD
                     IVAELKPVKRPIEPVEEPLELKNAVSAQTETMLADWRQLAQVSKKSWNDAIRTFVNKL
                     TEEYAEQYPEKGNYIAQVVDEIVKKDMRRLIIETGKRLDGRALDQIRPITCEIGILPR
                     AHGSALFTRGETQSLGSVTLGTKLDEQIIDDIDREEFTKKFMLHYNFPPFSVGEVKPM
                     RGTGRREIGHGNLAERALKTQIPSEEEFPYTIRIVSDILESNGSSSMATVCAGSLAMM
                     DAGIPVKETVAGIAMGLIKEDDKLIVLSDIIGAEDHYGDMDFKITGTRTGITAIQMDL
                     KIQGIAASTMRGALEQARVGRMQIIDIMEQTISKPREKISDFAPRMLTLKVDNDLIGE
                     IIGPGGKNIRALQTESNSKIEIDDNGMVFISAADFASAEKARQLIIDMIAKPEEGKIY
                     HDCPIKRLESYGAFVEFLPGKEGLLHVSEIAWRRTEDINSVLKVGDKVDVLLKKIAAP
                     GRFELSMKELVEKPEGYQETPRNSHQPRQSHSDRSKGDHHSSHRTHPRY"
     gene            complement(25500..25769)
                     /gene="rpsO"
                     /locus_tag="PLO58_02345"
     CDS             complement(25500..25769)
                     /gene="rpsO"
                     /locus_tag="PLO58_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQO73660.1"
                     /translation="MTLTKEIKSELVSKYGKNSHDTGAITAQIAMLTERIKDLSQHFA
                     AHPKDHSSRRGLMKMVSRRKHLLEYLRRKDNAAYKELIEKLELRK"
     gene            complement(25766..26734)
                     /locus_tag="PLO58_02350"
     CDS             complement(25766..26734)
                     /locus_tag="PLO58_02350"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQO73661.1"
                     /translation="MNIYYGIENIPADYKGACVTLGVFDGLHLAHRQIIQKVIELATE
                     QNSRAMLITFEPHPRRVLSQPLANHLPILTTVDEKIELLEKIGIEEVLFIKADQTFLN
                     TEESVFVQEILVKKLKVSAVVVGYDYHYGRDRKGNPQTLTAAGKKFGFRVIVMPPFAK
                     NEILVRSSLIRELITEGKILTANQMLGWQYSIKGKVVRGTGRGHKLGFPTANLVVNPP
                     DKLIPGDGVYFVKACLSNFEYFGILNIGMRKTFNESERVIELFIINFKDEDLYHREIK
                     VIFIERLRDELKFETSEALKKQMQADLEICLAKINKLENKTEVCFK"
     gene            complement(26731..27462)
                     /gene="truB"
                     /locus_tag="PLO58_02355"
     CDS             complement(26731..27462)
                     /gene="truB"
                     /locus_tag="PLO58_02355"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQO73662.1"
                     /translation="MPRISKSYSKKYINRKRIIKTDWSNKILNVYKPAGISSYQVVRR
                     VKNLLKIKKVGHSGTLDPFAEGVLLILIGGATKKMNDLLKLPKSYEAILRLGEMTASG
                     DNTAPIIKTAAVPSITPEKLAEIAKIFIGVIEQIPPIYSAKKINGRPSYKYARKGQEI
                     KLQPTQVNIYELDLRLLDYNSIFIEVTCSSGTYIRKLGEDLAEALGTVGHLTNLKRTR
                     IGNYDWKEAVPYSELNGYLLENATV"
     gene            complement(27389..27763)
                     /gene="rbfA"
                     /locus_tag="PLO58_02360"
     CDS             complement(27389..27763)
                     /gene="rbfA"
                     /locus_tag="PLO58_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQO73663.1"
                     /translation="MTGYRIKRVEDEIKRIVAEIFIRDIRLDNVGLITVTKVVCSSDL
                     REAKIYMSIYNNQKYSIAEAMKIIKSHTSFVRGLLGNRIMMRFVPRITFYYDETQEYA
                     ENIEKLFQKIHQPEEDNQNGLE"
     gene            complement(27763..28044)
                     /locus_tag="PLO58_02365"
     CDS             complement(27763..28044)
                     /locus_tag="PLO58_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HQO73664.1"
                     /translation="MTIGLLQIELRLKNTHSLKDRRSIINRIKNQVRAKFNVSIAEIE
                     PNDQYGHCLLGITTISNDQKIVHQTLNSVEYLVENFAEIQIIGREMEMF"
     gene            complement(28061..30589)
                     /gene="infB"
                     /locus_tag="PLO58_02370"
     CDS             complement(28061..30589)
                     /gene="infB"
                     /locus_tag="PLO58_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQO73665.1"
                     /translation="MSIDPKDKPKRIFEIAKELNISHNDIIKYLEKEGIPCKSIMTPV
                     DESSYMKILGEFAKEKDIVERLRLERARREAENRRKAEEDARRTAEMERRKLEEEVYK
                     SALLFFNSAIKDVFKYSLNVQSQLIESVWETAAVTDRLPEIPKEPEITEIAEPSIEKT
                     TTTTPPLESPKKKGKKKLRRIKIDELETRLDLKHGKHKPTELEDEVSHRRKKPGTEQE
                     ANDRVVDAQIRKTMAKMDEKSTKKRHKKTETVAEESEVSKKIRISDFATVDSLASLIG
                     IDAADVIQKCIELGTFVTVNQRLDFDTISVLCDEFGFEVEKMEHYGEDQIKIEDTKED
                     LENATPRPPVVVIMGHVDHGKTSLLDYIRRSNVVAGESGGITQHIGAYKVELPNKKFI
                     TFLDTPGHEAFTAMRARGAQVTDIVVLVVAADDGVMPQTIEAINHAKAANVPIVVAIN
                     KIDKPEADVERVKRDLANYGVLVENWGGKVQTAEISAKTGKGIDHLLELILLEAEMLE
                     LKANYQTLAKGIVIESSIDKRRGPMATVIIKKGTLKIGDPFVCGAAFGKVRTILDERS
                     QKLTKAEPSDPVVISGFDFPPKPADIFAVVQDEREARKIATERQKIEHEHQKRQVKEW
                     SLDTISAQIAEGKIKQLNVILKGDFDGTVEAIGQTLQDLGNEEVAVNIIHKAAGNITE
                     SDVLLAKTSQAIVIGFNVSANPKVNDLAKKENVEIRHYSIIYELTDDIKAALEGLLTP
                     EKIEEHLGEAEVREIFKIPRVGEVAGSYVIEGKVTRNATARLKRNGEVIFEGAVESLK
                     RFKDDVREVAEGFECGISLQGAPEFKVGDRIEFFEVKSVKRTLA"
     gene            complement(30594..31985)
                     /gene="nusA"
                     /locus_tag="PLO58_02375"
     CDS             complement(30594..31985)
                     /gene="nusA"
                     /locus_tag="PLO58_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQO73666.1"
                     /translation="MVNREIIEAFSQIAREKNVDRNELGDIIEKVFLTLIEKKYGSTE
                     NFDVIVNMDKGEIEIYHQMTIVDNPTGSPHEITLENARKTEPDLQVGDPFIEVIDPMS
                     FGRRLISHAKQILNQQIVRVEKNQIYEEYAKKIGQIVIGDVHQIRRDAIFILIGKAEL
                     KMPREEQIPNERYRRGENIKAIIKEAIMGPSGPEIIVSRADKSFLVKLFENEVPEIYD
                     KTIEIKKVARHAGDRSKVIVESNDRRIDAVGACVGIKGSRIQAIVRELNNEKIDIINY
                     SSEPEILITRALSPAKPLELEIDEEKKTALAIFADDEISVAIGKMGQNIRLASEVTGY
                     TIDAIKKSDYELTESETLYLEDIEGITEHNWKLLYSYGIETAEEFLDTPREELLSIKG
                     LGEKTLDKLEPIIIEAQEKRKAEVATIHEAAKAQREAALQEAQENKEKQTVEEETDLS
                     DEDIIDDFGQEEK"
     gene            complement(32008..32466)
                     /locus_tag="PLO58_02380"
     CDS             complement(32008..32466)
                     /locus_tag="PLO58_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73667.1"
                     /translation="MELETTKNAARAIVDKIVERFDAYVVGFKVNRQGYRTFVKVLIT
                     TDHGIALDQIAEISRAVRDDVAFNELFPDNFQLEVSSPGIDFPLRNFRDFHRNLGREM
                     NVFHHSENLKSPLKGKLESVDEDQLVIANAEGTHTLKMGEIDFAKVIIQW"
     gene            complement(32552..33247)
                     /locus_tag="PLO58_02385"
     CDS             complement(32552..33247)
                     /locus_tag="PLO58_02385"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO73668.1"
                     /translation="MLKPKKKITRKELKKDPVLEKISQIENLVRKQRKLVSYILLGVV
                     VVVLLTILLVNSKKKANTAAMGELGLAEMSFARNDYDDAILRLENLIEKYSRTRSSGM
                     ATILLARCYLAKDDYENARVNFEKYIDDYKDDKFLTAGAYSGLAICAEQDSNFALAAE
                     YFEKGGKISPHKFHKHELLLNSARNYLKVNNLEKAEEIVNIILNDEPESVNKNSAELL
                     LAQITTRKGLALK"
     gene            complement(33276..33348)
                     /locus_tag="PLO58_02390"
     tRNA            complement(33276..33348)
                     /locus_tag="PLO58_02390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33312..33314),aa:Arg,seq:cct)
     gene            complement(33488..34642)
                     /locus_tag="PLO58_02395"
     CDS             complement(33488..34642)
                     /locus_tag="PLO58_02395"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HQO73669.1"
                     /translation="MNVTKRFLRDEELAVYLQQLDIKLEKVDDNYRAEALILHQNDPE
                     KGYPKLIKNMQIYSQVAITELIVKTIPVHVMLNKTRRPSLVSSSFGENTDTIKKTCEL
                     CKLDRGQRGIIIKVGNEQEFIILTNPGITYPGDLTIAALKHDKQLITGRFNDMIQISR
                     ALYHFSIYFNGILAGASSPHFHFQAGYKDKLIGEIQIQKLLAGQSVGQARLKRILKSG
                     ELEVFYIENFLRPVHIVVTKNAEALLKFFDHYLILLKDVSQNIKGIKNVPDYGSLIPA
                     LGMRENEPRLNIMLKYFPEYGGFILALFPKRYNRPSCYFRGGNSQILIGMGIKEALGN
                     IITGRETDYKRLRNNPALISQIYTETSISPESVEILNQNLKKINILPRNR"
BASE COUNT         9805 a         7505 c         6329 g        10762 t
ORIGIN      
        1 acttataaaa tatacgataa ctaccacttt ttaggttact atccgctttt attttaattt
       61 ttcctcgtaa ggtatctatt tcgaatttac ttaatgataa atagttattc ggatctgact
      121 cactggcaat taaaatatcg tcatattgaa tattttgatg ggtaagccaa acataagaag
      181 tgtcaagatt gataaattct tcgacgcctg ttttatctaa aacagagtaa gtggatgtaa
      241 tttgagcaaa ttcatcttca tcattccatt caaaattcgg ctcaccgctc gtaggtatgc
      301 catccccttc tccataatca tttgtacctg gtttgccatc agagccaaca tcgcaagtta
      361 aataattcca atcgtcatca ttatcaattc cgtcggttcg agtgtcggtg aaattcactt
      421 tataggtatg cccatttaat agtgtttcat caataatttt atactgaata acgccggttg
      481 ccggaccttc caggtgtttc agggctaatt cactattttg ttcatcataa ccggcaactt
      541 tcgcagttgg tctaacttga acggtgttta tatcggtaat taccgtccca tctgactgca
      601 ccgaaataaa cttggtattt tccgatggaa aaatatcatt actggcgtca ccgtgatcat
      661 aggcgactac cgcataatag tagattcgtc cgttctgaac atcttcatca acgtaggaat
      721 gcactaaacc attgtcagag cctaaatagt aagaataacc gccagcactt tgatacaaat
      781 ccgaactcgc ctcgaaaatt cctttgattc cattgacctt atcaaattga gccagtggct
      841 tgtaaccagt cagcacacca ttggcatcgg taataacaaa tacatcattg aaatcaggat
      901 cagtagcttt atagatttta taaccttcga aatctttctc cttagttact ggatcgatgc
      961 tttcttcagc ctttctatcc caataaaggg ttaccttgcc gtcaccggct acggcggtta
     1021 ccgtcggctt atccggcggt tggggaaaac gataatcact gttgtaaata tcctgaaccg
     1081 tttcccgatt cttgagtaga tcggtcaaat catagccata tactaaagcc aatgaaaaac
     1141 gctgggtatc accggctcgt agtggaaaat accccgaacc ataaatgaaa tcgccgtctt
     1201 ccccggcaac tggtttgcca ttttgaatac tcgacggcac atcaaaatat cccggtttta
     1261 accattccca gagtaattca tcatccgcca ttggataagc attagatgga acaaaataat
     1321 taaaactagt taaaccgatt tggtccgatt catcaacatc ggttttatcg aaattcggtt
     1381 caccgagtgt aggcttgccg tcattctctc cataatcatt tgtacctggt ttaccatcac
     1441 taccgacatc atctgtttca ggattccaat caccgtcatt atcaagtcca tcattgcggg
     1501 cttcgtcaat caaaggatca tcaattccca atccccttcg ataatcttta taggctaccg
     1561 gatttaaggt gtcaaataaa actactccgt tttgatccac ccggcgttgt ctataatgca
     1621 acaggtaatt ctcgtcaatt aatccgtcca gatcattatc aatgccgtca taaacatttg
     1681 gattaattga accatttgcc aggacatttc cttctactaa ttttgtttgt cccgcaatga
     1741 ttgtgatttg catccctttg gttacaaccg tttctgtatc actggtaatt gtgtgaaaag
     1801 tacgcttata atctttgtcg attaatacta tctggtctcc tttattatat acaactgagt
     1861 cgaaatctga ttcggtaaat aggggtgccg taaaaattaa atttttacca ggatgttctt
     1921 caggtttatt atcaccgtca ttatcaatac cgtcataagg attaccggga ctttccagaa
     1981 aagcatatcc gaccagtcca accggtcctt gccacttcgg attacggctg ttattattag
     2041 ggtaatcacc agtataagtc aaatcccgtt caacatcaaa gaaagaataa tcgtcatcat
     2101 attcctgagg tgaatcatct gtaccagtca ctccgttgta agttccggct atcattccga
     2161 agaccacttt atcataatcc gtagtgcttg tattggtaat ttcataaagc caaaatatta
     2221 catcctgtgc cagaaactga gcccattgca ttccccgaac cttcacttcc aagccgagtc
     2281 cgttgcgatt cgcatctcta ttatcaggtt tgaaatagac accctttaga ttgtgaccac
     2341 tgttgaattc aacatcatta ttatcgtcca taacaaaata actttcttga tcagcattgg
     2401 ttaccccttt tccaaaaaat ccgttccatt gttctggcca acccggatcc tcaggatcat
     2461 ccattttatc aggccaataa ggcggccagg aatatcggtc ggtgctcata gctattcctt
     2521 gagaagactc attaaaataa ccggaaaccg gctcaaaagt ccattgatca ccagtaggcg
     2581 attcttcgcg agcagttgcc ggacgatcta ccggacaaac tacaacgcta tggaatatta
     2641 ttgtcgagtc aagttcttca gaatgataat ttacttcagc gcctaccatt ggactgacat
     2701 cgccaatata tccattggta tcaaaaagcc atgccccacg cggacctttt tcccttggtt
     2761 ggccgatgac tccccagttg ccaaaaactg tccgaaccag attgccgtta tggatggaac
     2821 tacgccggta agctttccca ctttgattgg caaaaattat ttgtgacgtt acaatcgtca
     2881 ttaacaaaat tgagagaatc tcaatagtaa gatattttat cgaatgtttc atatacttat
     2941 cctcattcct atttcgactc gacgtggttc ggaataatac gttgggtcgt taaaccactc
     3001 atgaaccgaa ttaatcggag ttttaatatt taaagcttct actcgggctt catcggtcgt
     3061 aaaaccagct cgaccggtct cgtcataaac attaacttca ttcaatttat caaataaatt
     3121 ctgaattctg ataaataatt caaccgaata tcttcctgat aaggtgaaac gatagtaaga
     3181 tctagcatca attgttaggg taggtggttt agtaccagca ttagttaaaa gtgatgtaat
     3241 atcttcgctt gattgcggag tatagggcaa accacttccc cagcgaccga taaaatttat
     3301 tccataattt ttgtgattat atcccactgt tccattaatt gtatggcgtt gatcccagtc
     3361 taagggatta agttgaattt ctggttggtt tccaccagca atcgcattgc ggtatgcttc
     3421 cggatctgat gcagtgcctt tagcaatttg gagcgtataa tcaaaactgg tatataaacc
     3481 actcggattc tgctgattaa gtgctatagt aatacctttt actacaccaa aatcactatt
     3541 aactaatcgg ctataagagg ccgctccgcc ataaacgata atctcttctg ctctcgttcc
     3601 ggtcaagtct cttatgtctt tcagataggc agtaatgtca actgaaaaat tatcacttaa
     3661 ttgttgctga agtccgattt ctcctacgat agtttgttct ggcttcaaat ccgcatttcc
     3721 aattactcct tgattaccgg ttccagaacc aagatcaaaa tcaggattca tatataaata
     3781 gttaaaattg gggatttgga aaaaatgacc ataagaaaaa tgaaataccc cgcgatcact
     3841 aatcggaaat gacaatccga ttctgggact aaattgatac ttgggagtgg cttttttata
     3901 ccaatattgt cttcgttcct ccaaagtgac ttcaggttca ccgggatcct ggacgccgtt
     3961 tccattaaga tcatgataac gattttcagg tttgattggc tcgtagattt caggatcgct
     4021 gggatccgca agcacaacac catccggttc aaaataatca aatcttaccc cgatattaat
     4081 gatcaactcg ctaaactcca ttttatcttg taaatatccg gcaaattcat aaggtttatg
     4141 acggtaatag ctagcataaa tcgtactatt atccaaaata gtcggatgaa tatatggatt
     4201 ccaggtagat ggtatattca atccggatgc gaagtctata taggtctcac cagtcgctgg
     4261 acgaatatta aatgttctta atgaaatatc atgctgttga aattccaaac cgccctttat
     4321 ctgatgttgt ccatttatct gggaagtcaa atcccatttg gcaaggtatg ttctactgtc
     4381 acgatattcg tgatcatttt gagttccacc aaaataaaat tgataaggat attgataaag
     4441 gtaagccggg tggacatagg tggtgctatc gttgtaactg gtatcggcag acaatctgcc
     4501 ggtatattta cgataataat atgagccagc cagagtgaaa aaggttttag aacttaattg
     4561 ttgctgaaac tttagtaaat gcgtttcagc ggttaggttt ttcttcaaca ttcctgccgg
     4621 attatatttg aacatacgat cataatcctg gtaatctttg ctatcgtaaa agaaattgta
     4681 ataaatacta cttcggttag aaagccggta gattaattgt ccttgaaaat ataacttttc
     4741 gttccagttc atattaaccc acttttgatc tcctaacata gtagttgagt ccagcaagaa
     4801 atgcgaacca tcgggagaca cagctaatat actcgtcggt ttataacgat tctgaccatg
     4861 tagccagcca ccaaagtaaa tatagcgcgt attgaaattg taaaataact tatcctttat
     4921 taatggtcca aaaaaactga tttcagcatt atggatattt agaggattta ttgatccaat
     4981 attatcaaag atttcgtcat gtgtactaag atgatctcca atatacattt ctagcgacgc
     5041 accgtgcttg ttgctacctt gcttggtagt gatattgacg atgcctgaca ttacctttcc
     5101 gtattctgca ttaaacgctc cggaaattac ctgcaattcc tgaatcatat ttttattgac
     5161 atcaatgacc gcactacgat cataagaatc ggttaccgga ataccatcta ttaaataggc
     5221 aacttcacct cttctgcctc cccgtaaact cccatctaca tatccggctt gcatttcaag
     5281 aacgtcgcta acttcagtca ctggtaaagc ggctaattct tcagctttaa cgtttgaagc
     5341 ggtcgcagtc atatctttga caatcatttt gcgctctgca gtaactatca cttcttcacc
     5401 ctctaaaacc tgagtcgaaa gcccaatatt gatagtagtt gtcaaatcag ctgatacaat
     5461 cacgccggtt atcacttttg gctggtaacc aatcatttga gcttttaaac gatattcgcc
     5521 aggcggtaca ttaagaatca tgtatcgtcc atctgtatcg gatgccgcac ccaaataagt
     5581 atcctcaagc attatgttac aacctattaa aggaagacca gtttgactat cagtaactat
     5641 accagtaatt ttaccagtat tacctccgaa taaataccct gcagttaaaa tcagaacaac
     5701 tgttattaac caatccttat atctcatcct ttacctcaat tgaatttgat ttattgattt
     5761 tatcaaagac aattaccctt caataatttt ataaactact attcctttcg acccgagata
     5821 atttgtatcg gtattaactg taaaaatctt ttcgcatttt gtgacatcaa agtttatctc
     5881 gctcattttg ataggtttat cattcaaatt tgcaaagatg tagatggtct cagtaccata
     5941 tttacgctca aatccgaaaa tccctttttt atcgtcagtc aatattgttt tatacctgcc
     6001 tcttcttaga cattcatgat tttttctgag atttattaca gatttataga aattgaacaa
     6061 atctgtatca actttaacgg aatctacagg tctttttatt ccaaatggat gagtagtctc
     6121 cggttcatat tgcatatcat cccaaaccat tggttttcga ttgtcgggat cgtctgctcc
     6181 ccacataccc acctcatcgc cataataaat ataaggtgct ccgatataag taaattgaaa
     6241 aacaagaatt gtcttttgga tttgtcggag ctcatccgaa ggacggttta tcttaaaatc
     6301 tttattgtaa accagattgc caccgtgatc gatccagcgg tctggattta ataacatact
     6361 ggcaaaacgc tcattatcgt gactaccaat tatattttgc agttgatatt gagcgggtag
     6421 cggatatttt tctctaatat cgcctaataa ttgatcaagc tcggatggtt ttatcctcat
     6481 ttctctgtca ataaaggctt taagcatagc atcgccgaaa cgataattca tcacgccgtc
     6541 aaaaatatcg ccctgcaacc atggtgaagc atcgaacatc ttattattca taaaatcttc
     6601 ccaccatatt tcaccagtca aatatgcctc gggatttatg cttttcaccc atttgcgaaa
     6661 atcacgccag aaatttttac tgaccttctc agccacatcc aatcgccagc cgtcaatccc
     6721 gtccgacgga tcgccatcac catttggatc accccatctt tcaaccacgg cttttatatg
     6781 cgcccgaaat ccttgtacag gaccattctc gtcttcccag atctccggta aatccggaat
     6841 tccattccag ccttgatatt caaattcatc ttcgattgta ctcggatcat caaaagactt
     6901 tatcacaaac caatcggcgt atggactggc ttctccattt tttttgacgt cctgaaaagc
     6961 ccagaaaggt atgccaacat gattgaatac tccatcaatg attattttca tttgacgggt
     7021 atgcacctcc tgtattagtt tcagaaataa tttatcagcg gaagtccaca gccaggtcga
     7081 aggatcatta ggattttcag tttgccaaat tgctatatca ccagccggat caggcccaaa
     7141 attattatca atatgatgat acattgtagc accgtattta tgcgacgaag ccgattcaaa
     7201 gactggattc aaataaattg cattgattcc taaatctaca agataatcga gtttatcaat
     7261 tattccctcg atatcaccgc cataacgacg caattgagca ttatagtaaa agtcgcgttg
     7321 gttggatttt tcccaaggct ggagtttata ccaatcagca gtccatggca taatgctcca
     7381 ttgcgtttga ggttcatatg gccaggtgcc ttctaacgtt tcaatcgttg gatcattagt
     7441 cggatcaccg ttcctaaagc gctctgggaa tatttgatac cagatagcat cgtaagtcca
     7501 gtatgttcta tctgagaggt tgtcactcaa acaaatccca gctactattc cgacgacaag
     7561 caatcctctt gccagtgaaa atttgccttt tgggacttga ttttctgtcg cgtttttcaa
     7621 ttgtagttct aatctgagtt actactaaaa gaatataggg tattaaaata tttaagtcaa
     7681 tagttttttt tactagtttt tgtccgagct aaaaatatgg tattcacttt tggtgataat
     7741 aggcgggaag attacaatcc cccactgtca aaattggatt agatgtatct ataacttcaa
     7801 taaatctaat cttgaataac cttaaactcc gttaggaatt aaacctgctt tacccgggat
     7861 tcaatcttga gtcaaaaatc taaagactac gccaaacccc gccggggttg aacaatatta
     7921 ccggtaactc cacctaatct cgaagaggga gtgttgacat gattagccta aagcttgctt
     7981 aaatgtgctc aacaatcctg tgaattacaa aaaactatta cgcgttaatc taatctattt
     8041 ctaaaatgtg gatataagga tgctacgaag gattataaaa atttatcaat ttttgatcat
     8101 atataatgaa tttttgataa aataaaaaag ccgctcttgt aagcggctta tctatagtgt
     8161 ttcggtgatt ttgaggtttc cctccccgaa cgtaaaagag ggggattttg agggatatta
     8221 cggcacatcc ggggaggggg aggggaaatc tatcttcttt tggacattat cattagatca
     8281 aagaacagga cttaatttac aaattttatg ccaatattca aatagcaaat ataattcgtt
     8341 agacaaaata ttttaagcct ggttacaata aaatttatgg aaaatctaca actagtgaat
     8401 ggtttctcag agttttactc atcttaaaat tatattttct tttttcatat aactttttat
     8461 attgataatg attaattgtt tagtgaaatt atagagtaat gagaaaaatt ttagcagttg
     8521 atgatgatcc taacattata gaattgatca catcctattt ttcatctcgt ggatttgaag
     8581 tttacgcgac tacagaagga gcacaagtac tcgatctagc tattagtaaa gaacccgatt
     8641 taattattct tgatattaat ttgccggaca ttgatggcct ttcagtcctg aagactttaa
     8701 agaatcgtcc aatcacggct tttatcccgg tgattatttt gactgcgaaa acgtcggccg
     8761 attttcaggt tgatggattg gttaccggcg cagatgatta cgttaccaag ccttttgatc
     8821 tcaatgttct ctatgctcga gttctcaatt cactacgacg ttcgctactt cccactcgtt
     8881 tcaaacacga tcaatttaac ttgttgtcat atttactcag agaatattca aaacgaggtt
     8941 acaaagtcta ttccaaatta ctggataatt atagccctca cccgccctac tggagaggtt
     9001 acatacctga tatgatcatt gagaaacccg aaaaattgcg ttgttttaat tttgagacca
     9061 ctcaatcaat tatggaagaa tcttttctgg atcgtctgaa ttctctggta gaatgccata
     9121 aaaataccac gtctcatttc gaggctacag ttatcgttcg gactatggat aactacaaaa
     9181 tcgtgcagaa attagtaaac gagcactgtt taccgataaa gataaaatat tttaaaaaac
     9241 atatccgccg gaatctatca gtttaagaag ctgataactc attccgaatt atttctaaca
     9301 tttcttctaa tttaattggt ttagtaaacc atttcttccc agatttgctt aaacggtcag
     9361 taatctttat atcatcatca taaccggtca taaagacaaa tttaatacca gggtaatcca
     9421 ctattacttg gtcgtaaaga tcaaagccgt tacgaaaagg catcacaata tctaatagaa
     9481 ttaaatccgg ctttttagcc tttatggatt ccaaagccgt ttcgacattt aaagctgtcg
     9541 ccacttcaaa tccattttgt tccaagaaat ccccgtagat ttcacgaatt accgcttcat
     9601 catcaacgat taatattttg ggttttattg gcatttaggc tttttgtttt tcgagtcctt
     9661 tacccatcca tttccttgct attaataaag caatcggact aatgattgcg ataaagccat
     9721 aaatcagcca catcgtctca gtattagttt gttccggtgt aactccggct gggcaatatt
     9781 tcgtcaggaa ccagccagaa taaagcgagg taatgacttt ggtcagaaac cagggaaact
     9841 gtccaatccc catataaatg ccggtcatat tcttaggggc gatttcagct acccactgca
     9901 gaaaacgcgg ttgccagata gcctctccga ttgtcataat gattagatag ccaaacaccg
     9961 agtagatatt tggaccaatt gccaataaaa aagtcggtag agccattata gtagtgccta
    10021 aaatcatcat tttataagtg tctttttggg cggttaaagc cgctaccatt ggagccagaa
    10081 taaaaatcag gataggatta aaattgacaa agaattcaaa attgttggaa accacgcccg
    10141 aaaaagctcg ttcgaaatac atcggtagag tcagccaatt atgagcaaat agagtctgaa
    10201 ccggaattag aatgaaaatg aaaaacataa atcggccgtc tttaatgggg aaatttttta
    10261 agtaataaac aatcttttcc ttggttgatt gcgtttcagt cgtttctgga tcagaaactg
    10321 gttcgtcctg tccttcccga gcattggcaa tcgccatctc gacggtcttc ttcgataaaa
    10381 tgaaggcaac tactaaaatc ccaactaccg ttaaagctac ataaacccaa tatacaccga
    10441 caattccatg atttttcctg attggcggcg aaattaatcc tggcaggaag ccccccagat
    10501 tcataattgc atagagcatc gcatagccca tagcggaagt ttttttgtca gtgaattgct
    10561 tgaccgcggc ataacaggcg ggctgataaa tgccataacc taagactact cccaatattc
    10621 ccaacatagc aacgatatga gctttttgcc aaagtccggg agcaccggtt tgaggtgcta
    10681 atgttagaag cacacgccca accaacatcg ttgaaagtga aatcaataaa gccttccgca
    10741 ctccgatcca atctaccgtc gctcctaaaa ataacattgc caatgttatc ccggcagttt
    10801 gaatcccgac catttgtcca gcctgtatat cgttcagtcc gagatcattg aaatacattg
    10861 ctaaaagact gagaatacca aaataggtga ttccctccag caaatacgat aaattaattc
    10921 cccataaagc gcgaggcgcc ttaatcaaat taacaaacgg atcaacgatt tcccgtaaag
    10981 ggttctgaac cggtttatta tcttccatct aagcatcctt tatttttact gactctgtcc
    11041 aataactgaa tctataaaat attgacttat cattccaata aaatttttta atggcaaaac
    11101 ttctatcgct aattttgtat gtcagtgatt atttattatt aatattgcat taaggaatgt
    11161 aaatgaatat tcagaaatca attatgaatt gctcaaatat tgttatttaa atttttagaa
    11221 cttagactag tgctgtgcta aatatttctc aaaaccggca gattgaacct tctggccatc
    11281 ggcgcaaatc cagagcactt cgcaattggg gaagttttcc agagcttttt gtccggctaa
    11341 agcgcccaaa acaaataatc cagttgccat tgcgtcagct tgctcagcag ttgcggcaat
    11401 tacagttgtg gattgacagg agtcagccgg taaaccggta cgtggattta agatatgatg
    11461 aatgcgtttg ccttcataca taaaaaattt ctcataatct ccggaagtag ccactgcccc
    11521 actgtcgaat tcaattacaa gtagaatatt atcggcttga cgcggatcct gaatcccgat
    11581 tctccacttt ttcccatcta cacgcctcag tgctcgtaaa tctccgccgg cattgaccaa
    11641 tgcaccagcg acattatgct ttgcaatgac tgcgacagct tgatcaatac cgtagccttt
    11701 ggcaaccgca cccattccaa ttttggtttg gtgattttta gcacccaatt gnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccctcggga ttgccttccc agaattgggc
    11881 ggaaatgcgg ttgatttcgg caaacccggc ttcaagcgca ttattggctt cggcttcggt
    11941 cgtatctaat acggttattt cgataaccgt tcccatagcg aaacgcgttg attgagcctt
    12001 gatcagcttg cttttaggtt ggcaacttgt caacagaaaa acacaaaata aaaagtaagg
    12061 aatagggatt ttgaaattaa gcattacttc ttctgcggaa tcattcaaac ttgccattta
    12121 atgtctgacg caaacgaaag acattaaatc cacttttggc tttacgttta ctccaaaaat
    12181 aaatgataaa cccggtcaag ataactacta taattccaca aaacatctgc acaatgtccg
    12241 ctggaattcc aaaaatgcta tgtttaataa atcggttagc gattagtact ccgaatagaa
    12301 aaccaaataa aggtaaacca tattgaacta tggttaattt tagctgatta gcaccgattt
    12361 gctcgattaa aaccgtatcg cccactttag cattaatggt attaggtatt ttgagtatgc
    12421 gcttgcctgt agaattgggg cggcagatca ttttcgcacc gcaagaatgg caggtatctt
    12481 tttctaccag ttcgaccgtt acccagtgct ggtcagcatc aatcaccgtc gccgtttcat
    12541 catcagtttt gaaatattgg tcgttagtgt tcatccttag ttccaaggat tacaatagct
    12601 ttagttgggc atttttcggt cggcagaacc agttccttgg tataaacgtc gtgatcaacc
    12661 acggcaagat tatccacgac tttgaagcct tggccgttga cggctttttc acacaaaaga
    12721 caaccggtac aagcacgctt acaaaccgta cgggcaattc taccgatgtc acgattccta
    12781 caaagcacaa aaacatgatg actgattgga tgcaattcga taatgccccg tggacaggca
    12841 gtaacgcaat tgccacaacc ggtgcatttg gcgcggtcta cgaccggtaa gttattttca
    12901 ttcatatgaa tagcgtcaaa cggacaaacc gtcacgcaat ctccaagtcc aatacatccg
    12961 taactgcagg cacgttcacc gccactaacc aaattagccg cagaacaagt tttgatgccg
    13021 cgatactcgg cttttttagt agcgacatcg tcgccgccct gacaaaaaac taccgccact
    13081 tgccgctcgc cgatttcgac attcactccc aaaatccggg ctatctcctc ccgggctgat
    13141 ggactgcaga ccggacaatt tgagatttgc gatttaccac cggctaaagc ctcagcaaaa
    13201 ttattacagc cggcatagcc gcaactgccg caattggcac ccggtaaaca ctcagcaatc
    13261 aagctgattc tcgggtcttc ttcaacgtgt agttttttat tagctactgc aattaatatg
    13321 gaaaagaaca aaccaatccc gcccatactc aataaactgt ataaaaacgt tgtggaattc
    13381 atttcagata ccttttgaga ttagattaat ccagaaaatc ccataaatgc cagggctaaa
    13441 caaccggcga cgattaaagt aatcggtgct cctttaaaag gttcaggcac atccgccagt
    13501 tccaactctt cccggatgcc agccataatt accagcgcca aggtaaagcc tgccccggaa
    13561 gcaaaaccaa atacgatact ctcaacaaaa ttatattgct tcatcaccag gaaaagtgcc
    13621 agccccagaa tggcacagtt ggtcgtgatc agcggcagaa aaataccgag cgcatcatag
    13681 agcggacggc tgactttttt aataaacatt tcaactaatt ggaccagcga ggcaattacc
    13741 aagatgaaag atacaatttg gaggaattcc aggtgtaaac cgaccagaat atacttattc
    13801 aataaccagg taactgcggc cgttatcgtc atcacgaagg tcgttgccat tcccatacta
    13861 accgcagaac cgagtttttt cgagactccg atgaagggac aaattccgag gaatgacacc
    13921 aagacataat tattgataac cgcggctgag atgaaaatta atatcagatc catttttact
    13981 tcctttttgt attaagccaa ttggtaaatc cgattataaa acccaacgtc aagaaagcac
    14041 ctggttgcag agctatagcc atccagggtt gaaatgacaa tggcataatt tgatgcgaaa
    14101 aaatagtacc attaccaatt agttcgcgaa atgatcccat tatcagtaat accagagtaa
    14161 acccgacact cattccgagc gcatcaataa cagaacgacc aactggattt tttgaggcaa
    14221 aggcttcctg acgagcaaat acaatgcaat tcacgacaat taagggaaca aaaggtccca
    14281 gcgccttgct catttcggga taaaaagccg ccagaactcg gtctgccacc gtcacaaaag
    14341 tcgcaattat gatgataaac gtcgcaatcc ggacttcgtt gggaatcaat tttcttagaa
    14401 gagagatcat catgctggac ataaccagcg taaatattac tgccaatccc atcgtaacgc
    14461 cattaatagc tgatgttgta acggctaaag ttgggcaggt tcccaaaacc atacgcagga
    14521 tgggattctc gttccagagt cctttgagaa attcctgtgt tactgaaatt tttgatttcg
    14581 ccatattatt tcaccttctc taaataaagg gcgcggtttt tttcaatagc ggcgttgata
    14641 atatctacaa ccgccctcga agaaatggtt gcgccggtaa tggcttgaat ttgcgattta
    14701 cttttatctg cggcttgtcc cttgacaacg gaaatcgggc ttactgctgg taaatcagca
    14761 aattgcttta aaaaccagaa cgcatccgtt ttgttcgttg gatcgttttc aatttttgtc
    14821 ccgagtccgg gagtttcggc ttgggataat attttcattt gtacaatttt agtcatttcc
    14881 ggatccatac cgaccagaat gcgtaaacgg ctctgaaatc catcaccctc tgccacaaac
    14941 gccacgccga ttacctgccc tttatcatcc ttaccaacat aaaaagtaac gtctttgatg
    15001 accttcacgt cacaggtttt ttggcctggg attacatatt cgatagcttc caataaggtc
    15061 ttattttcgt ttaattcaat acgcggagca gtgtaggtat ttaacacagc caatattaat
    15121 ccggataata aagccgtcag ggttaaaaca actaccagtt tgagtgactt attcacgatt
    15181 ttacctcccc aaaatatttc ggtcgagtat aacgattaat caatggaaca aatccattca
    15241 ttaataaaat tgaatacata acgccttccg gcagacctcc aaattgacga ataagaacaa
    15301 ccaaaattcc cgctccaatt ccgaaaatcc aggtccctaa attggttatc ggcgaagtca
    15361 ccatatcggt cgccatgaaa aaggcgccca acattaatcc gccggaaaag agatgaaaga
    15421 tcggatcggg atactgtgcc ggattaatga gccaaagaat cccactgaaa actacaactg
    15481 tgccaagata actcaccgga atgcgccaat tggatatatg cannnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nntgcccaag gccaccgaaa atttgtttac cgattataat
    15661 ggaaaaagcg gcaccgatta tgaccatact aagcggtaac cccggcggta aacacaatgc
    15721 taataacaag ccggtcaagg cggcactgcc atcataaatt gtcattttac gacgtctaat
    15781 taaattaaat aattcctcgg caatcatagc cgtaatcata gaagtggcta taactgtcag
    15841 acaacgccat ccgaaattgt aaatactgaa aataaccgtt ggcagtaaag tagccacaac
    15901 cagccacata attgaagaaa catcagtaga ggctttaata tggggcgaag atgaaagaat
    15961 aaaaagtcgt ttctcaactg acttgtcaac aggatttgga gtcggtttta ctttcggctc
    16021 ttttccggct ccatctttaa ctatattctg ctgttctgcc attatgataa atttccttat
    16081 taaatattcg atattgaacg atatttcata cgcttacggc ttttttttca gttctctttt
    16141 gacgctgaat ttcggctacc cgcgttttcc cagttcggat atgatgaact aaaggaattg
    16201 atgctgggca aataaaagcg caagaaccac attcgataca attagctata ccatattgtt
    16261 cggcttcatc ccacttggca aactgcgata agcgcgccag cctggttggg attagattca
    16321 ttggacagac atggaggcaa gccccacatt tgatacaggg atattctttc ctttcgacag
    16381 cgattttatc cgttaaacag acaattccac tggtagcttt tataaccggt accgctaaag
    16441 aagcctgagc aacgcccatc attggaccgc ccatcactac cttgaccgta tcgtcggtca
    16501 gtccgccaca gaagtcaatt aaattctgaa atggagtgcc gattctggct aatatatttt
    16561 ttggttcgcg cactccctga ccggtaaccg taacgactct ttcaatcagc ggtttgcgtt
    16621 ttgtaacggc ttcagccaca gccagagccg tgccgacatt attaacaaca acgcccacgt
    16681 ccattggtaa tccgccggcg ggtactttgc ggtttaaaac ggcatctatt aacatcttct
    16741 cggcaccttg gggatatttt accttcagag gctggacaat aataccccag aatttttcgg
    16801 tagctttggt tagcgccgca atagcatccg gtttattggc ttcgatacct attattgccc
    16861 gactgactcc gagcgctttc atcataatgc gaagtcccag gactacctct tccgaatgtt
    16921 cgaccataat gcggtgatcg gctgtcaaat aaggttcaca ttcacaaccg ttaaggataa
    16981 aagtatcaat ggttttattt ttgggcggat ttaatttcac ttgcgttgga aaagccgctc
    17041 cgcctaagcc aacgattccg gcgtctaata taattttatt gatttgttcg ggagtaagat
    17101 tttcccaatc gctaatcgaa tcaggtaatt ctacccattc gtcgtgacca tcgccgatga
    17161 tgtttatcat tagcgaatta tttcccaaag ggtgcggtaa tttttcaatc gtttttactt
    17221 ttcccgaaat tgaggcgtgg atattgcttg atataaatcc gccgcttttg ccgatcaatt
    17281 gaccggtttt aacttccata cctttttcga ctaccggtac acatgcggct ccaacatgtt
    17341 gctgaagcgg aataaacact tccgtcggta aaggcggttt ttctatgctt ttataattac
    17401 ttaaactttt aaactctgct ggatgaacgc ccttcttgaa cgtcttcaat ttcattttaa
    17461 tcttgctcct actctaatca ggctctaaaa gtcaaaaaat cgaggaaatt taatcaaaat
    17521 attccaaata ttccttaaat aatttggaaa gatcctgaca ttcgatagtt tgtttataaa
    17581 ggagaaagtg tgtaaatttc tatgcttttt gacgggggcg aatggattcg acgggattga
    17641 agatctgccg aatagcgtgt cgtggttttc aggagggcca cgttaataaa cctgagaatc
    17701 gaaaatgcca attcttttgg caccgtcgct tacgcttaag taagtgaccg ttctgggccg
    17761 gtctcgcctg caggctggtt cagagcgtcg tcaaagcagg ctcgttccgt ttatcggttt
    17821 gcgtaaacgg aattaaaaac agcaaactgg cttttaccaa gtttgtccgc tggcacggta
    17881 aaagcgagat tcagcaacgg actacacacg tagcgattct gcagttctct ttttcggacg
    17941 ggggttcaat tccccccgcc tccactcata ctttcgttaa agatggaatt tagatggctc
    18001 tcccagagat catcccaatt atttttttga atgatagatt tatataatac atcgctccgc
    18061 ggttgattat ctgtcaaata ttttactatg cggttagcat tatattccgg actctcatcc
    18121 agcggcaagt atagagcacc ttctttcgct acttctctaa ataccggtat ttgactgagt
    18181 gccaatggag ttctggcgat acctgcttca agaattggta aaccgaaacc ttcatccatg
    18241 cttaaataaa gcactaaatt actgataaaa taaaggtcac gaaccacagc tcgattcttt
    18301 tctgccggga catttaatct ctcgaaaaga tcgccgagaa aaaacaggtt attggacagg
    18361 ttgttttctt gaaccaaatt ataaagactg gcggcgtatt ctgtcaaagc gccattctcc
    18421 ctcttaggca ttccggtaat gataccgaca agattaggcc aggtattccg cagtgccgca
    18481 attactttaa tactgcgttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 gcaatcagta attgtttatt aggcttcaca agaggtttac taaatttgct ccggatttca
    18661 ttcgtaagaa gaattaattt tcgtatttta atgattgcca gttatcaaac agaagaccgg
    18721 caaaattttc agtcgagtca atcgctaaat attgcttaag atcactttcg ctttgaactt
    18781 taaaagtcgg gaaaagttta gtcattttga attcgatacg atattgatct aaaatgtctt
    18841 ctacccaaag tatattaccc attttactgg ggtgatataa tgccggtgaa atcaaacatc
    18901 cattatccac ataagccata ggtaactgcc cggtagcaaa cggtaaatct ataagcgcca
    18961 gcggtggagt actaataatt attttagttt tcttgattaa tgcatcacag gcatcaaaaa
    19021 ctagattagc aattattccc gaacgccatt ctaaccaatc cggaagcatc tcgacgattt
    19081 ggctggtaaa agataccact tcaccaacca ttgatgaaaa ttctgtaacg cagaacgggc
    19141 agtaacaata gggtggtacg tgatgctgta tatccagatt ttcttcctgc cagaaaaagg
    19201 ggaatctcag attttcaaac atacagtaat caatttgccc cagacgataa gtcttttgaa
    19261 gcaataaatg ataaaaatca tgtccaatgg gattgttggg acaaaccgga taatatttat
    19321 ttctcggagt ataagcaatt ccaagataat taaccggtgg agaaaaaata tccatttgcc
    19381 acaaaaatgg attttggaaa caatctaaaa taattccagc ttttaagccc gccagtttag
    19441 tcagttcgat catccttgcc accggaaaat atttaatcgt caaatcaccg gtttcataag
    19501 gcttgtttat acaataaaac acgccgtcat atcctttatc acggataatc tttaagtctt
    19561 caatcaattg gtgacgttcg tatttattgg gataatattt gataaacatt ggcatttcgt
    19621 tctgaatttc gtgaaaaatt tatttaattc aatcgaaatt tgtcggagcg agtggatttg
    19681 aaccaccgac cccctgaacc ccattcaggt gcgctaatcc ggactgcgct acgctccgct
    19741 tgtcaaaaac gtcagtaatt taattgatag gaatttattt atctacgaaa aattggatta
    19801 ccagttatta agcatttcca gtaattcgtt gagccccttt ttaatttccg ataataccat
    19861 agcggtatcg gttctctccc ctactgtttc atccaattcg ttcctattct cagtatcaat
    19921 agctttttgc caatcttctg gtttcatttc tttcagtttc tgtttagcgc cttctatagt
    19981 aaaacgttta tcatacaata ggtgtttgat atattgaatt aattggatgt ctttttcacg
    20041 ataagcgcgg ttgcccgccc ggtttttagt cggtgttaat gatggaaatt ctttctccca
    20101 gtatcggaga acatattgct tgagcccggt aatttcactg acttctccaa ttgagtaata
    20161 taatttttta attggctttt ccatatcaca cctgttatca ctattattta aaccattact
    20221 ttaatagtat aatcagttta aagggttatg tcaagttatt tttttattta tcgcaatttc
    20281 tggacgataa tagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaatgg
    20401 attccaccta gattaatgtc ccaggtattt ggccgatcta aataatcggt gatgataaaa
    20461 atagttgccg ggatacccag actttttaat atcggaaagg catataaata aatatcctga
    20521 taaccatcgt cgaaggttag agcgatcaga ttcggtttat ttttattagc ctgaagattg
    20581 ctaagagtgg caaattcata accacgatta agcaaaaact cgatatgttt ttgaaatttt
    20641 tctggtttga tgcaactcgc gccgatttcc gaacgccaat caattttatg atagacaagg
    20701 actcgattct ttatgggaac aaactcgtct atggctccac cctttgacta ctccggtttg
    20761 aaattatatt tgcggtagaa ttttgcggta aattcgccga ttagcgtata agaatgacgc
    20821 agaatatttt catctggtag aaaaactacc cgaaaatgat tagtccctcg ttgctgaccg
    20881 aaaccgctac cgtaaactac tagtactccg gtatcattga gcaattcgag gacaaattgt
    20941 tcatcagtaa cgccgggcgg caattgaatt cggggaaaag cgtaaaaagc cgcctcgggt
    21001 ttaacgcaag aaatgccagg aatttcattt aacattttgt ctgtaatatc acggcgaatc
    21061 gtcagttttt gaatgacttg gggtaaatga gagtcattac cattcagggc tggagccacc
    21121 atatattgtt gcggatgtac cgcacaaagt cttgcccgca agagtttatt aatggcttcg
    21181 cggtaatctg ccatcacagc ggtaggatca gaaaatattg cccaaccaac ccgccaacca
    21241 ggcatgatat aattcttact caatccacca aaggtaacta cgggtacatc atcagctaat
    21301 gaggcaattg aggtgtgcgt ttgaccgtca agaagcaatt tatcataaat ttcgtcagcg
    21361 aaaatcacta agttatggcg acgggcaaaa ttgatgacat ttaataaact ttcgcgatta
    21421 taattcgcac cagtcggatt gttaggatta atgacgacaa ttgccttagt gcgcttgttg
    21481 acccgctttt ctaattcgtc aaaatctatc tgccagccgg ttgattcatt taataaatat
    21541 ggatttaagt taccatgata aaccgggatt tggccgctat aaagtggata gccaggcgag
    21601 ggcgtcatga tattgtcgcc gggattaatt aaagccgcta tcgccagcgt aatggcttcc
    21661 gaagctccat tagccataaa gacatcattg gctgttaccc cttttattcc ttttttttca
    21721 gtagcatagc gagcaactgc ttctctggct tcgattaaac ccatcgaatc gccatagacc
    21781 gagacattca attgggaatt ggcactcgat tgtaaaattt tttcacttac aatctggcgc
    21841 agttcaaagg gagtttcaaa gtcataaact ggaggatcgc caatatttaa gtatataatt
    21901 tttctgccct ggtcttctaa gtgcttggct ttcacagcta tgtcacggat cgcataagtg
    21961 actttttcgg tccgccttgc tggaatgatt ttctgattca tataattttc tcttttatga
    22021 ctttttgcca tttatcataa tcaggattct atcggctgaa taatggtttc aataaaggcg
    22081 ttccggtcga ataagtagcg tgccaattcc tgtaaatcct caatgctgat tttatcaata
    22141 atttccaata attcggcaat cgtaataata tgtttttcgt aaatttctgt cttagctaaa
    22201 ttactcattc gagcgttcat attttccatc gccagtacca gtccaccctt tacttgctgt
    22261 ttggcttttt tatattcacg aacgggaatt ggtttacgat aaagcgcggt catttcatta
    22321 ttaattttat caataaccat atccagtcgg tttttatcag aaccagcata tactccgaat
    22381 aatccatctt tcaaaaaaag gtctgaaaat gagtaaacct cataaacgta accatatttt
    22441 tcacgaatgt tatgaaataa acgcgatgtc attccgccac ttaaaatcat attgagtaaa
    22501 ccgagctgga atcgtcgcga atcgccttgt ccgaaaatcc ggcgtcccaa aataacatgg
    22561 ctttgcatta tcggtttggg ataaattttc tgggtttctg gttgagtatt acaggaatct
    22621 ttttcttgat aatcggcacc atttcccata tcttgacaat atttctcaac cagttttact
    22681 aattgttcat gattgactct tccggcggct gatacaacta aacgaccggt cgtataatga
    22741 cgcttaagga aacgttttaa atcagacacc tgaagggcgt tgacactttc gactgttccc
    22801 tggatcggac gccccattgc ttgatttggg aaaagctgct gaataaataa atcgtggatt
    22861 aagtcatgtg gagtatcctc gatttcgcgg atctcttcgc aaataactct tttttccttc
    22921 tcgagttcgt ggggatcaag ccgaccatgc aaaataaggt cagataacac ctcaacacct
    22981 ttagcaagat tttcatgtaa caactgcaca ttaaagcaag tcacattccg gctggtaaag
    23041 gcattgatga ttccacccag cgattcaatt tcatgcgcaa tagcaaaagc tgaacgttta
    23101 tgcgttccct taaaaaccat atgttcaagc aaatgcgcaa taccgttttc attgtcttct
    23161 tcgtcttgag aaccagcttg tatccagaat cctaacgaaa ctgaataaac cgaatcgaca
    23221 tattcagata ggactcggat tccgttaggt agaattgtac gctggatatt ttcggaaatc
    23281 atcgcttaat agcgggggtg tgtccgatgg gatgaatgat gatcaccttt tgaacgatca
    23341 ctgtggcttt gccttggttg gtgagaatta cgtggcgttt cttgataacc ttccggcttt
    23401 tcaaccaatt ctttcatcga aagttcaaaa cgtccggggg cggcgatttt tttcaataga
    23461 acatctacct tatcgccgac tttcaagacg ctattaatat cctcagttcg acgccaggca
    23521 atttcagaaa catgcaacag accttcttta ccaggtaaaa attcaacgaa ggcaccgtaa
    23581 ctttccagcc tttttatcgg acagtcatga taaatcttgc cttcttccgg tttagcgatc
    23641 atatcaataa tcaactggcg tgctttttca gccgatgcaa aatctgcggc tgaaatgaaa
    23701 accataccgt tatcatcaat ctcaattttc gaattacttt cagtttgcaa agcccgaata
    23761 ttcttaccgc cgggaccgat tatttcgcct attaaatcgt tatcaacttt taaggtcaac
    23821 atacgcggtg cgaaatcgga tattttttct cgcggtttgc tgattgtctg ttccataata
    23881 tcaataattt gcatgcgacc gacacgagct tgttcgaggg cgccacgcat agtcgaagcc
    23941 gcaattccct gaattttcag atccatctga attgcggtaa tgccggtacg agtaccggtt
    24001 attttgaaat ccatatcgcc gtaatggtct tccgcgccaa tgatatccga taatactatg
    24061 agtttatcat cttctttgat taatcccatc gcaataccag ctaccgtttc tttaaccgga
    24121 attccggcat ccatcatagc tagcgaaccg gcacaaaccg tcgccattga agacgagcca
    24181 ttcgattcca gaatatcgga gacgatccgg attgtgtagg gaaattcctc ctcgcttgga
    24241 atttgagttt ttagagctct ctcagccaaa ttaccgtgtc caatttcccg tcggcctgtg
    24301 cctctcattg gtttgacttc accaacgcta aatggcggaa aattgtaatg aagcataaat
    24361 ttcttagtga attcttcgcg gtcaatatcg tcaatgattt gctcatccaa tttcgttccg
    24421 agagttaccg aacccagact ttgagtttca ccgcgagtaa aaagggctga gccgtgggct
    24481 cgtggcaaaa ttcctatttc acaagtaatc ggtcgaattt gatccagtgc gcgaccgtcg
    24541 agtcttttgc cggtctcgat tatcagccgc cgcatatctt tcttaacgat ttcatcaact
    24601 acctgggcga tgtaattgcc tttctccgga tattgttcgg catattcttc tgtcaattta
    24661 ttgacgaaag ttcgaatggc atcattccag gattttttgg aaacctgcgc caattgacgc
    24721 cagtcggcta gcattgtttc cgtttgagcg ctaaccgcgt tttttaattc cagcggttct
    24781 tcaaccggtt caatcggccg tttgactggc tttaattccg cgactatatc tttctgaaac
    24841 tgaataagtt cccgaacggc agaatgtccg aactcaatag cttcaattaa caaatcttcg
    24901 gaaattattt tacattcacc ttcgaccatt acaaccgaat cttcggtacc agccacaacg
    24961 atatcgagtt caccttcttc catctgcttg atagtgggat tgagaataaa ttctccatta
    25021 acccgaccaa cccgtactcc ggcaattgga ccggaaaatg gaatttctga aatcatcaaa
    25081 gctgtcgaag ctccaataat accgagtaca tcagcgggat tttcctgatc gcttgagata
    25141 acgttcgcaa ttatctgagt atcacagtga aaatcttccg gaaatagagg tcgaatcggt
    25201 cggtctatca accgtgaccc cagaatttcc ttttccgatg gacgtccttc gcgtttgaaa
    25261 aaaccgccag gaattttacc agcggcatag gctcgctccc gatattctac ctgtagaggg
    25321 aagaaatctt gttcggtaac ggcatcaaaa ttggctaccg ctgtcactaa aatcatcgta
    25381 tcggcataac gaacaaccac ggctcccgaa gcctgtttag ccattcggcc ggtctcaata
    25441 cttaattcac gacctccaag ggtcatcttt ttttggtaca attatttact cccaattatt
    25501 tattttctta attcaagttt ctcaattaat tctttataag ccgcattgtc tttgcgacgc
    25561 aaatattcca aaagatgctt ccgacggcta accattttca tcaggccgcg ccgcgaacta
    25621 tgatctttag gatgggcagc aaaatgctgg cttaaatctt tgatcctttc ggttagcata
    25681 gcaatctggg ctgtaatcgc tccagtatca tggctgtttt tcccgtactt ggatactaat
    25741 tcacttttga tttcttttgt caatgtcatt taaaacatac ctccgtttta ttttctaatt
    25801 tattaatttt cgctaaacat atttccaaat cagcttgcat ctgttttttt agtgcctcac
    25861 tcgtttcaaa ttttaattca tcgcggagtc tttcgataaa aataacctta atttcacgat
    25921 gatacaaatc ttcatcctta aaatttatta taaataattc aattacccgc tcagattcgt
    25981 tgaaagtctt tcgcatccca atattcaaaa ttccaaaata ttcgaaattt gaaaggcaag
    26041 ctttaacaaa atagactcca tcacctggga ttaatttatc cggtggattt acaacaagat
    26101 tagcggttgg aaaaccgagt ttgtgaccac gacccgttcc tctaacaacc tttcctttaa
    26161 tactatattg ccagccgagc atttgattgg cagtaaggat tttcccttcg gttataagtt
    26221 ctcggattaa cgatgaacgt accaaaatct cgttctttgc aaacggcggc ataactatta
    26281 ccctaaaacc gaactttttg cccgccgcag ttaaagtctg gggattgccc tttcgatccc
    26341 gaccataatg ataatcatac cctacaacga cagcgctcac ctttaacttt ttaactaaaa
    26401 tttcttgcac gaataccgat tcttcagtat ttagaaaagt ttgatcggct ttaataaaaa
    26461 ggacttcttc tattccaatt ttttcaagta attctatttt ttcatccact gttgtcaata
    26521 tcggtagatg attagccaac ggttgactaa gtacccgacg tggatggggt tcaaaggtaa
    26581 ttaacatagc tctgctattt tgctcggtgg caagttcgat aaccttttga ataatctggc
    26641 gatgcgccag gtgcaaaccg tcaaacactc ccaacgttac acaagcgcct ttataatcgg
    26701 caggaatatt ttcaatgccg taataaatat tcataccgta gcgttctcca gcaaatatcc
    26761 atttaattcc gagtagggca cggcttcttt ccagtcataa ttgccgatcc gggtgcgctt
    26821 caggttggtc aaatgaccca ccgtgccgag cgcctctgca agatcttcgc ctagtttacg
    26881 aatataggtt ccactcgaac aagtaacttc aataaaaatc gagttataat ctaatagcct
    26941 caaatccaat tcataaatat ttacttgagt aggttgcagt ttaatttcct gtccttttcg
    27001 ggcatattta taagacggac ggccattgat tttcttggcg gaataaattg gtggaatttg
    27061 ttcgattacg ccgataaaaa ttttggcgat ttcagccaat ttttccggtg ttattgacgg
    27121 aaccgcggcg gtcttaataa tcggagcagt attgtcacca gaggcagtca tttcacccaa
    27181 acgtaaaatc gcctcgtaag acttgggcaa tttcaataaa tcattcattt ttttggttgc
    27241 cccgccaatt aaaattagga gcactccttc cgcgaacgga tcgagtgtcc cgctatgacc
    27301 gacttttttt attttaagaa ggtttttaac acgacgtact acctgatagg aagagatacc
    27361 agccggttta taaacgttta aaattttatt actccagtcc gttttgatta tcctcttccg
    27421 gttgatgtat tttttggaat aacttttcga tattctcggc atattcttga gtctcgtcat
    27481 aatagaaggt aatgcgtggt acgaaacgca tcataattcg attaccgagc agaccacgca
    27541 cgaaggaagt atgactcttg ataattttca ttgcttctgc aatcgagtat ttttggttat
    27601 tgtaaatgct catatagatt ttggcttctc ttagatcaga ggagcaaaca actttcgtta
    27661 cggttattaa gccaacatta tcgagccgaa tatcccgaat gaaaatctca gccacgatgc
    27721 gtttaatttc atcctcaacc cgttttatgc gataaccagt cattagaaca tctccatttc
    27781 cctgccaata atctggattt cggcaaaatt ctcaaccaga tattcgacag agtttaaagt
    27841 ttgatgcact atcttttgat cattactgat ggttgttatt ccgagcagac agtgaccata
    27901 ttgatcattt ggttcaattt ccgcaatgga gacattgaac tttgctctta cttggttttt
    27961 aatgcgattg ataattgaac gtctatcctt taaagagtgg gtatttttca ggcgcagttc
    28021 tatttgtaac aatccaatcg tcataacaat ttacgcccat ttaagccagc gtccttttga
    28081 ccgatttaac ttcaaaaaat tctatgcgat cgccgacctt aaattcgggt gctccttgca
    28141 gtgaaatgcc acattcgaaa ccttctgcga cttctctgac atcgtctttg aatcgtttca
    28201 acgattcgac agcaccttcg aaaataacct ctccgttgcg ttttaaacgg gcggtggcgt
    28261 tgcgggtaac ctttccctcg attacataag aaccggctac ctctccaacc cgcggtattt
    28321 taaatatttc gcgcacctcg gcttcaccga gatgttcttc aattttttcg ggagtcaata
    28381 agccttccag tgcggctttg atatcatcag tcaattcata aataatcgaa taatggcgaa
    28441 tttcgacatt ttctttctta gctaaatcat tgacttttgg attcgcgctt acattaaatc
    28501 caattacgat agcctgggaa gttttggcaa gcagtacatc cgattcagta atattaccgg
    28561 cggctttatg aataatgttc acggcaacct cttcattccc aagatcctgc aaagtttggc
    28621 ctatcgcctc tactgtacca tcgaaatcac cttttagaat cacattgagc tgttttattt
    28681 ttccctcagc aatctgagcg gaaatagtat ctaatgacca ctcttttacc tggcgtttct
    28741 ggtgttcatg ttcaattttt tgcctttcgg tagcaatttt tcgtgcttca cgttcatcct
    28801 gaaccactgc aaatatatca gccggtttag ggggaaagtc gaaaccagaa atcacaaccg
    28861 gatcggatgg ttcggctttg gtcaatttct ggctacgttc atctaagatt gttcgtactt
    28921 ttccaaaggc ggctccacaa acgaatgggt caccgatttt taatgtcccc ttttttatta
    28981 ttacagttgc catggggcca cggcgtttat caatgctgga ttcgattaca atgcctttag
    29041 ccaatgtctg gtaattggct ttgagttcca gcatctcagc ttctaacaga attaattcta
    29101 ataagtggtc tattcccttt cccgttttag ctgatatttc ggctgtctgg actttcccgc
    29161 cccagttttc taccaaaacg ccataattgg caagatcacg tttgactcgt tcgacatcgg
    29221 cttctggttt atcaatttta ttgatggcta ctacgattgg tacattggcg gcttttgcgt
    29281 gattgattgc ctcgattgtt tgcggcataa cgccgtcgtc ggcggcgact accagaacta
    29341 ctatatcggt gacctgagca ccgcgggcac gcattgcggt aaaggcttca tgccccggag
    29401 tatcgagaaa ggtaataaat ttcttattag gcaattcaac tttatacgcg ccaatatgct
    29461 gagtaattcc acccgattca cctgcgacta cattcgaacg gcggatataa tcgaggagag
    29521 aggtcttccc gtgatcaaca tgtcccatta ttacgacaac tggcggacgg ggggtggcat
    29581 tttcgaggtc ttccttagta tcttcaatct ttatttgatc ttcgccatag tgttccattt
    29641 tttcaacttc aaaaccgaat tcgtcacata gaaccgagat tgtatcgaaa tcaagacgtt
    29701 ggtttaccgt tacaaacgtt cccagctcga tacatttttg gattacatcg gcggcatcta
    29761 tccctattaa ggaggcgagg gaatcaactg tagcaaaatc acttatgcga atttttttgc
    29821 taacctcaga ttcttcagca acagtttcag tttttttatg tctttttttc gttgactttt
    29881 catccatttt agccattgtt ttgcgaatct gagcatctac tacgcgatcg ttagcttcct
    29941 gctcagtacc tggttttttg cgacgatggc ttacttcatc ttctaattcc gtcggtttgt
    30001 gtttgccatg tttcagatcc agacgagttt cgagttcatc aatttttatc cgacgcagtt
    30061 ttttcttacc ttttttctta ggagattcta acggaggagt ggtagtagta gttttttcaa
    30121 tactgggttc tgcgatttca gtgatttccg gttcttttgg tatttcggga agtctatcgg
    30181 taaccgcggc tgtttcccag acagattcaa ttagctgact ttgaacgttt agactatatt
    30241 taaatacatc cttaatggca ctattaaaaa acagcaaggc tgatttatag acttcttctt
    30301 cgagtttacg cctctccatc tcagccgtac gacgcgcgtc ttcttccgct ttacgtctat
    30361 tctcagcttc acggcgggct cgctccaaac gtaatctttc gacaatatct ttttctttag
    30421 caaattcgcc cagaattttc atataagaag attcgtcaac cggcgtcatt atgcttttac
    30481 agggaattcc ttctttctca agatatttta tgatatcatt atgagagata tttaattcct
    30541 tcgcaatctc gaaaatccgt ttgggtttat cttttgggtc aatactcatt tgtttatttc
    30601 tcttcctgac cgaaatcgtc aatgatgtct tcgtcggata agtctgtctc ttcctcaacg
    30661 gtttgttttt ctttattttc ttgtgcctct tgcagggcgg cttcgcgctg agccttggcg
    30721 gcttcatgga ttgtagcaac ttcggctttc cgcttttcct gagcttcgat gattatcggt
    30781 tcaagtttat cgagggtttt ctcgcctaat cctttgatac tcaataactc ttcacgagga
    30841 gtgtctaaaa attcttcggc agtttcgata ccataactat ataacaattt ccagttatgc
    30901 tcagtaattc cttcaatatc ttccagataa agggtctcgg attccgttaa ttcgtagtcg
    30961 gattttttaa tagcgtcaat cgtatagccg gtaacttcgg atgccagtcg tatattttga
    31021 cccattttac cgatagctac ggaaatttca tcatcggcaa aaatagccaa agcggttttc
    31081 ttctcctcat caatttccag ttcgagcggt ttagctggtg aaagtgcgcg cgtaatcaga
    31141 atttcaggtt cactactgta atttataata tctatttttt cattgtttaa ctcacgaaca
    31201 atcgcctgaa tgcgactacc tttaatacca acgcaggctc ccacagcatc aattcgtcgg
    31261 tcattggatt caacgataac cttactgcga tcaccggcat ggcgagcaac ttttttgatt
    31321 tctatcgtct tatcataaat ttccggaact tcattctcga acagcttcac cagaaaagat
    31381 ttatcggcac gcgaaacaat aatttccgga ccgcttggtc ccattatggc ttccttgata
    31441 atggctttaa tattctcgcc gcgacgatag cgttcattcg gtatttgttc ttcgcgcggc
    31501 atttttagtt cggctttacc aattaagata aaaatcgcat cacgtctaat ttgatgaaca
    31561 tcgccaatta cgatttgtcc aatttttttg gcgtattctt cgtaaatctg gtttttctcc
    31621 actcggacaa tctgctgatt aagaatctgt ttagcgtgtg atattaagcg ccgtccaaag
    31681 ctcatcgggt ctatcacctc aataaacgga tcccctactt gcagatctgg ttccgttttt
    31741 cgggcatttt ccaaagtaat ttcgtgcggc gaaccagtgg gattatctac gatcgtcatc
    31801 tggtgataaa tttcaatttc acctttatcc atattgacga tgacatcaaa attttcggtc
    31861 gagccgtatt tcttttctat taaggtcaga aatacttttt caattatgtc accgagttca
    31921 ttccgatcaa cgtttttttc acgagcgatc tgagaaaaag cttcgatgat ttcacggttt
    31981 accaattttg gaagtctcct atatgtttta ccattgaatt attactttgg cgaaatcaat
    32041 ttcgcccatt tttagggtat gagtaccttc agcattcgct ataacaagtt ggtcttcatc
    32101 aacggattcc agttttcctt ttagcggtga ctttagattc tcactatgat ggaatacgtt
    32161 catttcgcgc ccaagattac gatgaaaatc acggaaattt ctcaatggaa aatcaattcc
    32221 cggcgaacta acttccagct gaaaattatc gggaaagagt tcattaaatg ctacatcgtc
    32281 tcgcaccgcg cgacttattt cggcaatctg atcgagggca attccgtgat cagtagtaat
    32341 taaaacctta acaaaagtac gatagccttg acgattaact ttaaaaccaa caacataagc
    32401 atcgaatcgt tctacgatct tatctacaat tgctcttgcc gcgttcttgg tagtctctaa
    32461 ttccatgcta cacttctttc aaactaaaag tgggctttta aacccactta ctatgcggca
    32521 aatttaggta atttaggctt attattgcaa cttattttag tgccaacccc ttgcgagtgg
    32581 tgatttgggc taataaaagc tcagctgaat tcttattaac ggattcgggt tcgtcgttaa
    32641 gaattatatt tacgatttct tccgccttct caagattgtt tacttttaaa taattacgcg
    32701 ccgaattcag gagtaattcg tgcttatgaa acttgtgtgg cgagattttt cctccttttt
    32761 caaaatactc ggcggctaat gcaaagttgg aatcttgttc ggcacaaatt gccaaaccag
    32821 aataagctcc agctgttaaa aatttgtcat ctttataatc atcaatgtac ttttcaaaat
    32881 ttactcgggc attttcataa tcatctttag ctaaataaca gcgtgctagg agaatagttg
    32941 ccatgccaga actacgtgtc cgagaatact tttcaatcaa attttcgagt cggagaatgg
    33001 cgtcatcgta atcattgcgg gcaaatgaca tttcggccag tcccagttct cccattgccg
    33061 ccgtgttggc ttttttcttg gaatttacca gtaaaatagt cagcaagacg acgaccacta
    33121 ctcccagtaa tatgtacgat actaatttgc gctgtttgcg gaccagattt tcaatttgtg
    33181 agattttttc taagaccggg tcttttttca attctttccg ggttatcttc tttttaggtt
    33241 ttaacatcta tttactcctg ttgtaatttt tcctgtaccc tcgccgggaa ttgaacccag
    33301 atcaaaggat taggaatcct ctattctatc catttgaact acgagggctc aaaaagcggg
    33361 taaagttata tttatagacg atattttgca agctacaata aagtcaacgg ggaaataata
    33421 atgctgtatc taatcaataa tagtttctga attcgttata aataattcgg cgccactctc
    33481 tcatgattta tctatttctt ggcaaaatat taattttttt caggttctgg ttcaaaattt
    33541 caaccgattc cggtgaaatt gaggtttcgg tatagatttg agatatcaga gccggattat
    33601 ttcttaaacg cttataatcc gtttctcgtc cagttattat atttcccagt gcttccttaa
    33661 tgcccatacc gattaaaatt tggctgttac ctccccgaaa ataacacgat ggacgattat
    33721 atcttttcgg aaaaagcgcc agaataaaac cgccatattc ggggaaatat tttagcataa
    33781 tgttcaaacg cggctcattt tccctcattc cgagcgccgg tatcagactg ccgtaatctg
    33841 ggacgttttt aattcctttg atgttttggc tgacgtcctt taaaagtatt aaataatggt
    33901 cgaaaaattt aagtaaagcc tcagcatttt tcgtgactac aatatggacc ggtcgcagaa
    33961 agttttcaat ataaaatacc tccagctcgc cagattttag aattcttttg agacgtgctt
    34021 gaccaaccga ttgcccggct aatagtttct ggatctggat ttcgccaatt agtttatctt
    34081 tataaccggc ttgaaaatgg aagtgggggc tcgatgcacc ggctaatatt ccattaaagt
    34141 aaatcgaaaa atgatacaga gcgcgggaaa tttgaatcat atcattaaat cgccccgtta
    34201 tcaactgctt atcatgcttt agggcggcta tggttaaatc accgggataa gtgattccgg
    34261 gattggtcag gataataaat tcttgttcat tgccaacttt aattattatc ccacgctgtc
    34321 cccgatcgag tttacatagt tcacaagttt ttttgattgt atctgtgttt tcgccaaatg
    34381 aactggagac cagactcggc cgacgagttt tattcagcat cacatgtact ggaatcgttt
    34441 ttacgattaa ttccgttatc gctacctgac tataaatctg catattttta atgagcttgg
    34501 gataaccttt ttcgggatcg ttttgatgga ggatgagagc ttccgctcta taattatcgt
    34561 caactttttc taacttgata tctaactgct gtaaataaac cgccagttct tcatcacgta
    34621 aaaatctttt agtaacattc attctttaaa gaccttgttc tgttctatga accattccta
    34681 ttagcctatt ttttaaataa tctgatgata agctacgcaa ttatgatttc agaggcaatt
    34741 attagaaagt cttagtgaaa taatcaagaa tttttatgtt tactaaactt aaaataggtg
    34801 t
//
LOCUS       DAOMJU010000013        34372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1073_length_34372_cov_19.879797, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000013 DAOMJU010000000
VERSION     DAOMJU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 34372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34372
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_34372_cov_19.879797"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLO58_02400"
     CDS             complement(<1..368)
                     /locus_tag="PLO58_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HQO73670.1"
                     /translation="MEAKTTKKAWSKEDLEYFKNLILKKREETLKAIERLEATTVSDN
                     TRNDNTAMDSAYAYHMADVGTDSQEREKAFLWLARENKFLSHLNSALERIKTGEYGVC
                     IECGELIMKERLEEVPHTQH"
     gene            complement(395..3535)
                     /gene="ileS"
                     /locus_tag="PLO58_02405"
     CDS             complement(395..3535)
                     /gene="ileS"
                     /locus_tag="PLO58_02405"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQO73671.1"
                     /translation="MIKKVPSSVDFVAIEHEILKFWEQNQIFKKLVAQNANGPKWSFI
                     DGPITANNPMGVHHAWGRTYKDIFHRYKAMNGFRTRYQNGFDCQGLWVEVEVEKELGF
                     KSKRDIEKYGIEKFVERCKERVRKYSKIQTEQSIRLGYWMDWKNSYYTMSDENNYTIW
                     LFLKKCHENGWIYKGHDVMPWCTSCGAALSEHEIATEGYKEITHPSITVKFPLINRPG
                     ESLLVWTTTPWTLTSNTAAAVHPDLNYLKIRQGNEILYVVEGRERDVIKGEYTIEGKI
                     PGREMLGWTYHGPFDDLPAQKNVKHVVIDWEEISASEGTGIVHIAPGCGQEDHELGKI
                     HDLAVLAPLDEFGNYLEGFGWLTGRNVDGISRDIFKDLEAKGIKYRTDSYLHRYPVCW
                     RHGTELIFRLVDEWFIAMDGVRQPMMDITRKIRWIPEYGLQHELDWLRNMHDWMISKK
                     RYWGLALPIYECQDCGQVTVIGSREELKEKAMEGWDKFEGHSPHRPWVDNVQITCPRC
                     GAKVSRIPDVGNPWLDAGIVPFSTLHYTTDRAYWEEWFPADFICESLPGQFRNWFYSL
                     LAMSTVLENREPFKTILGYALVKDEKGQDMHKSAGNAIWFDEAAEKMGVDVMRWLYAD
                     HNPFNNLNFGYNIAQDYRKRLITLWNSYSFFATYAELDGYSPQKHQVSDENLSELDRW
                     LMARLVQLIRLSREALDDYNVARVMKGVDDFLEVLSNWYIRRSRRRFWKSDNDQDKWS
                     AYQTLYKALKGLILILAPVVPFITEKIYQNLVRGLEPDAPESIHLAKFPQAEEEFDEA
                     LLKKIDTIIEIVSVGRAARNKANLKVRQPLAQMWVKLPAEVTANDLVDLTDQILEELN
                     VKALDFITDETQFVNIQIKPNYAQLGAKYGQRLPAIRQAIAELNPIEVARRLEKSENL
                     ELNLIDGKIWLSRDEIIVERKENEGFAIAESNGITVILDTKLTPSLLQEGLVRDFIRH
                     VQTMRKEADFQVEDRIQVNFEAPADFTAAVHNYIEYFCNETLAQKVVDNFAPGEYEKE
                     LKIENNKVRVSLTRIK"
     gene            complement(3550..3771)
                     /locus_tag="PLO58_02410"
     CDS             complement(3550..3771)
                     /locus_tag="PLO58_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HQO73672.1"
                     /translation="MNQVGKLLIIFGVVLIVVGVLLTVAGKFPFLDKIGRLPGDIIIR
                     REHFTFYFPWVTCLLISLIFFILFNLFRR"
     gene            complement(3946..5445)
                     /locus_tag="PLO58_02415"
     CDS             complement(3946..5445)
                     /locus_tag="PLO58_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HQO73673.1"
                     /translation="MKRKIGIVIGGILLVALGFTIASIIIRPGKVVFYEDKNSGMTTF
                     RPAAVTAGEDIPSLQAFNDAFVQIAESVTPSVVTITTEKVIKTNLRRDFPGFPDDDLF
                     WRFFGFPQGEMRSRALGSGVIVDQKGYILTNNHVVERGEKISVKLIDNREFKAEIVGT
                     DPLTDLAVIKIDAKDLQPAILGDSDALRVGEWVLAIGSPFSENLAHTVTAGIVSAKGR
                     SGVINNTNYESFIQTDAAINPGNSGGALVNIRAELIGINTAIATAGMSQGNVGIGFAI
                     PINLAKKVMTDLIEKGKVVRAWLGVVIGDVDENIAKSLGIESRQGAIISEVVKDSPAE
                     KAGLKVGDVIKEFDGKTIKDSAHLRTLVSTSEVGKPKTLVVLRGKKELKIDVKLGELP
                     ESVERGVYPDTKSDSKLGIRVDEINSVTASQYGISPDETGVVVTGVDRNGRAAGILQV
                     GDIIKRIGERDISNVAEYQKALEEVKTDIVLILIKRKNVTFFETIDLSK"
     assembly_gap    5677..5776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5777..6867
                     /locus_tag="PLO58_02420"
     CDS             <5777..6867
                     /locus_tag="PLO58_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQO73674.1"
                     /translation="TELSEFLTHFGRNSRFLRGAFHRFFVPELLEFFKQLGIPTVTER
                     GGRIFPVRNDANEIVTALVNWANSTGVIIRPNTRANQLVLTENRISGVIVRNPNSTTE
                     KINADAVIIATGGASYPGTGSTGDGYRLAKSAGHSITPVRPALVPLVTVGNLAACLQG
                     LSLKNVGIKVFADSRKIADEFGEMLFTHFGLSGPVILTISKLVVDALNAKQKVRISID
                     LKPALDDQKLDLRLQRDFAQHGKMQFKSVLKEFLPQKLIPVCLDLIKIPPEKTCNQIT
                     TAERRRLRLWLKNFNFDISGHRGFGEAIITAGGVALNEVDSQTLQSKIVQGLYFAGEI
                     LDLDADTGGYNLQAAFSTGWLAGQSAGR"
     gene            7069..>8372
                     /locus_tag="PLO58_02425"
     CDS             7069..>8372
                     /locus_tag="PLO58_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015408017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HQO73675.1"
                     /translation="MFLPTTKQEMEELGWQELDVILVTGDAYIDSPHIGIAVIGKVLL
                     KAGYRVGVIAQPALNVPDDIMRLGEPCLFWGVTGGSIDSMVANYTASLKKRRHDDYTP
                     GGINDRRPDRAVIVYTNLIRQHFKKTKPIVLGGIEASLRRIAHYDYWSDSIRRSILFD
                     AKADFITYGMSERSILELAESLQKGSDYRDIRGICYIAPHKPENTLELPSYEKVRDNP
                     KTFTRMFHEFYGNNDARSAQRLCQKYATRWLVHNPPSLPLSQAELDEVYNLNYERDVH
                     PYYAKQGSVKALETIQFAVTTHRGCYGECNFCAIAIHEGRTVQWRSPESVTNEVRSFA
                     TMPNFKGIVADVGGPTANMYGFECPQKLKTGGCKQKRCIYPEICPQLPVSHKKQIELL
                     IKLRALPGVRKVFVASGIRHDLVLADKKWGRKYLREVISEHTS"
     assembly_gap    8373..8472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8473..9766
                     /locus_tag="PLO58_02430"
     CDS             <8473..9766
                     /locus_tag="PLO58_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HQO73676.1"
                     /translation="PINCEPDDELSALGREIIRLQKEWQELSLLIGLTAQINAGLNLE
                     EVLDRIFDSFHAIIPYDRIGVALLTDDGKNVRSCCTRSRLPVIKLKEGYSARLKNSSL
                     EQIIRTGQPRIINDLEEYLAQKPRSTSTRLILSEGMLSSLTCPLIIQTKPVGFIFFSS
                     CNKNTYRDAHIELFQKIADQVSVIIEKSRLYQNLAALNEEKTKFLGIAAHDLRSPIGV
                     IKSYTDILLAGLVGNLTDQQTNIIKTIENNCTRMLNLINDLLDISAIESGKLTLQKTT
                     IEPSIYFTNYAENAMRLAAVKSIHFTTLIAPQIPPIEFDPNRITQVLDNLVSNAIKFS
                     SPETTITLAVKAIGKNLEIRVADQGQGIPAAEIPKLFKPFSRTSVQPTGGEKSTGLGL
                     AIVRRIVQAHGGKIRVKSQPGQGTEFIIHLPFISKSDN"
     gene            9964..10731
                     /locus_tag="PLO58_02435"
     CDS             9964..10731
                     /locus_tag="PLO58_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQO73677.1"
                     /translation="MRELKFKPGDIIINEGAYGSEAYILKAGSVEVSKMTDHKKVILA
                     ILQPQEIFGELGLIDDKPRSATVIARTAVTVDEITREDFSALLDDKMPFVIPIMRSFF
                     EHLRQANQLVLQMEAQITEASKTAPQPNAPTEIKLEGLTPIASQSLKNRTLILRKFPF
                     KIGRESGQRREDVFIDNDLSLIDNIPYNVSRNHMSINFYNGQYYILDRGSSLGTIVNK
                     IPIGGRLSNFKAELHKGENTVILGTSNSQFQFKITIE"
     gene            10728..>11804
                     /locus_tag="PLO58_02440"
     CDS             10728..>11804
                     /locus_tag="PLO58_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQO73678.1"
                     /translation="MIPRKNLARAFQKAIEQPNYAINNFVHRLKASFSYHFLNGNSLG
                     PETISLFLTYKCNLKCAMCGQWGTNGAFREYPSEVVRQQLSLDEIQNLIDDVRHYNPN
                     ITLFGGEPMLYPNWLKVVEIVKAAGLRCNIVTNGTYIGKWVREIADSRLDEIIFSLDG
                     PAEIHDKIRGVPGTFQKAINGLQELNTLKKTRNLNRPLVNINCTITELNYRSIDEIIK
                     IAEDIGACGLTLHHLLFLDRATVMEFIPFFQEKFGQTPTDWLGFIVDELPQIDPEYLI
                     AKRNELKTRRFDTDVNFYPNLEDNEIRQWYTQFQFKASSYKNRCQSLWATAYVFPDGS
                     VRPYHSMNFTPGNILQDKFSKIWNN"
     assembly_gap    11805..11904
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12121..12507
                     /locus_tag="PLO58_02445"
     CDS             12121..12507
                     /locus_tag="PLO58_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQO73679.1"
                     /translation="MEKILIVDDEQEIAELLEEVLEQLGYKTAMAFSKAQASEVLREF
                     EPRIVLLDIRLPDGDGISFLKEIKASQPAVEVIMITGLADRDIAIEALREGAADYICK
                     PIDLDYLANSLLANIVTRYNTDAPAL"
     gene            12562..13809
                     /locus_tag="PLO58_02450"
     CDS             12562..13809
                     /locus_tag="PLO58_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HQO73680.1"
                     /translation="MKRNQFLELPKKIILLVCITSLFSFAQKRESPISTDSYQNEMTL
                     SPGDRIKVSYIDIGPQGVPIEKSSILEVRHDGTIFHELLKVVYVQNLTIKQVEDLLTV
                     KFSEFFTQPQVVVSIVEKTTIKVFLWGGVDNIGVYRISPGTTLAEFIITHGGLTSDAN
                     ISKITLTRKDGSVIIFDLERYLFSNDETNNVELHDGDKIIVPRLIMKDEYARLSKGYI
                     LQAGNVLEITINELATMEKSPATPENYIIDQDGNIYHRRFGLVQLGGLTVDKAQATLT
                     ELAKKYYREPVVTIDVVKLTSRSVFVFGEVNRPGIYPIEGNIRLAEFLADIGGLTSNA
                     DPRKIIVTRENYKPVTFDLNEYLFDRKDEKNIYLEDGDRIIVLSRKRGFIVNLAEKIQ
                     PFTTVLYIISTALNIYFLITYIK"
     gene            13823..17011
                     /locus_tag="PLO58_02455"
     CDS             13823..17011
                     /locus_tag="PLO58_02455"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HQO73681.1"
                     /translation="MAHYELNLRDYWRIIKKRKIIIIVTLVLLTCFSLIFAIMNRPTP
                     LYRATASLKIEKSTNLTGLYMYSISWGTGDDLATRAEEIKSYPMIEKAAQKLGLIDSS
                     LTSEQIRSNKEYLDLVSRLKSRVKTEQEGYTNIINIIVTDYDPVLAANLANALAQVYE
                     VESFKEKNLQAQKALEAVRNQRAMAEQALRISEQKVQNYREQNKLATLDGAASRLTAE
                     LNSAETNLEKIRGDIHQIDNILREIGNNREYIYLANLNLLLSQPNRFLEDLQTQLNQI
                     RSQITNYSQYYTPQHPAIIELQQQLKNTENRFIHELEAFRQNLARAETIAEEKYKRLN
                     EEYKSLPLIELTLTNLERERDVNTQIYQQLEIRYQEAIIRYSEQVKEVFIIRPAFVPT
                     SPINQTTVGPTTAIGMIIGLILGIVLAFVAETLDTTFNTVDDIKEALNTNIMGIIPFV
                     DFQGLKSQLAEKAETPVPDEILDMQARLVSYYSPKSSIAESFRSLRTNVHFALLDKGY
                     KTLMITSSISNEGKTSIAANLAISMAQIGINTLLVDADLRKPRISKLFGIDREPGLTD
                     VILRKEPIDNVIRTMSDLMVGTMACDILRTDNIAGIEYLNILTSGKIERNPAEIAASK
                     LMDNLIAELKRRYDLVIFDTSPLIPATDSTILGTKVDTVLLVYYQGKISRGTLRRSKS
                     QLELLKTDILGVVVNGMKAELSADYADYKYGYEYHYQEESARPKNKVITALESFFLKP
                     AVGMTNGFFSQLNKLRAGLALIALGLIIGGGLWAFTKYQGKKAKSQVESKITETIIPQ
                     TTSSSDTSVASHILSSDTLPNLTAQTAPIIPSELKQLQKQYGIETISQQQVDQNLTES
                     GLLTNNSPTTVQSSIPPATQSDKQTEPLISIVYSSTILSKTDPAASVQPLPSSLPYTL
                     IIEQSFDKSTLINQLNKLRRNGLSAFITIDFSDPKQKNYLLCYGSFSSENEAINKSKE
                     LQFLGFRGTFRTIKLPFSILLDDFPTLQSAQQAIERFASIKDYLYIQPSSQKPDAKKT
                     YYLLFGGFPSTESTELVMNTYPELGHKTIVIR"
     gene            17023..17370
                     /locus_tag="PLO58_02460"
     CDS             17023..17370
                     /locus_tag="PLO58_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73682.1"
                     /translation="MPRHKHHKSRSNRKAIIFLVIPISLIIAITIIIIIREPNWPFLS
                     ILRQDETANQEKYMGSAVRVETPIRQKYETEWRNDYFETWQKTYEKILQNTDKNEIKE
                     LERELKASEKGIN"
     gene            17441..18799
                     /locus_tag="PLO58_02465"
     CDS             17441..18799
                     /locus_tag="PLO58_02465"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQO73683.1"
                     /translation="MLVALVLVKVDPVYAILAAVGLGVLIVTFTNTDFAIYILIFSML
                     LSPEIGSRTTSGEGMTIRIDDLILAIIILTWLAKTAINKELGLFRKTPLNKPIWIYTL
                     VCILSTGIGMMAGRVNFLTGLLFVLKYIQYFMIYFMVMNQIRTKKQINNYLISLIITF
                     TIVTIFALIQAPSGQRITAPFEGEAGEPNTLGGYLVLLIAINLGLVLNDDAIANRHLR
                     RGLLLITMISIYPLLMTYSRGSWLAAIAVIIAYIAISKKRWIIFGVVTIVFILAPYIF
                     PQPVIDRVKYTFTEQQGYDATLQEHIGGLTIDTSASERIRSWRAAFRDITKRPVLGFG
                     ITGWRFLDAQYIRVLIETGIIGLAAFVYLQFSLLSNIRKVYSDAKTPLYRALAQGFFV
                     GMIGMMAHSITANTFIIVRIIEPFWLACGLVMAVPEVEELETEKLPEIEKSPTTINLE
                     NR"
     gene            18805..19086
                     /locus_tag="PLO58_02470"
     CDS             18805..19086
                     /locus_tag="PLO58_02470"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HQO73684.1"
                     /translation="MGKIDLDFKPTTRPDLPFRELKDGGVVYEYRTDTLHSLNATAAY
                     IWVLCDGQHTVAEIITSIKQNYNQFETDPTSEVIKIIEKFRTLNLLTND"
     gene            19086..19934
                     /locus_tag="PLO58_02475"
     CDS             19086..19934
                     /locus_tag="PLO58_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73685.1"
                     /translation="MPLFKIQVGSAEISLECCQSIISEVTSSVFSAILTPSQISAKIH
                     YRIETKNSKFLLFRNNRLTHRNRQLSRIIYALEWQIVQDLLTKNKSVLKFHGAALKHL
                     DTGFIFLGAASSGKTSLAILLMRYGWEFFSDEFALVDCDRQIIPFPRNLIIKPHLRDK
                     INIPLDYPIFKLDDESDGKIDAHYISPSHFGTVYLSKIPLKGLVFLEKADRAGFDLQP
                     LEHHKAFRLLTQHLLNRNFSGEKWLDFLVNLLGSSAVYRLKISSPMDLTPSDSRALVE
                     QLNQIE"
     gene            19936..21069
                     /locus_tag="PLO58_02480"
     CDS             19936..21069
                     /locus_tag="PLO58_02480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQO73686.1"
                     /translation="MIKYFRDLLSDRIRIENFRQAIESTVNHNSIVVEIGSALGTYSF
                     FAAKAGAKAVYAIEMNDIFYVGRELAERNNLTDRIKFIHGRSTEIDLPEKADLIIIED
                     FGPFFYYHNLLEVIRDARERFLKADGRFLPARITLYIAPFESQAWRDELDLFAADNDQ
                     LFGFDWSYTTEIAFNQTYYADSHPKKLLSAPFLLKTIDLKNETNFPFQKQFEINIKEN
                     GQIHGLIGWWDCWFTDQQFFSNSPQSGNNSWGQLIFPFRYPINVKSGESVKISLMVLE
                     SSPSGLIDYKWKITHHSGSQEQDTFSGRFFDLQQFNPMRRDVIPSLYEKRLIQTFILN
                     QIDGKRSWDEIAEVVMKMFPEKFASKEDVFKIVLPLSNFLKGN"
     gene            21191..22129
                     /locus_tag="PLO58_02485"
     CDS             21191..22129
                     /locus_tag="PLO58_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQO73687.1"
                     /translation="MNLIIQIPCYNEEDTIEKTLNDLPRSIPGIDSIEILIIDDGCQD
                     RTVEIARQWGVNHFVHFNQNKGLARAFEAGLEKALELGADIIVNTDADNQYKGSDIAR
                     LVQPILAKKAEIVIGDRQIDKIEDFSWLKKKLEKFGSYVVRKVSQTNIPDVTSGFRAY
                     SREAALHMNIISTFSYTLETIIEAGQKDIPIISVPIETNKTERPSRLFKSNWYYIRRS
                     FATIVRIYTMYQPLRVFFYTGITSFGIGLILSIRYLIFMIVGQGKGHIQSVIIAAVFL
                     IIGFFLLMIGLLADVIAANRKLQEETLYRIKKDSLK"
     gene            22196..22780
                     /locus_tag="PLO58_02490"
     CDS             22196..22780
                     /locus_tag="PLO58_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQO73688.1"
                     /translation="MKNQEAEGLLSPLIKKIRLKKAAKCIQNNSTVLDLACGAGYLRQ
                     FLPSQCKYYGIDRITPPSLNLFNAFLQLDILQPESFETIKEWLPEPKVDVVTMLAFIE
                     HIKNPQYLLKQSLLLLKPNGLLIITTPHPIGRKLHDSLAKLYLCSLSGAKEHENFFGQ
                     QDLKFLLKQSGYKLLSYERFLMGLNQLIIATPLK"
     gene            22812..24113
                     /locus_tag="PLO58_02495"
     CDS             22812..24113
                     /locus_tag="PLO58_02495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQO73689.1"
                     /translation="MKYFSRIQNLLDKYEIPIIFAILLIGLVIRLAVIITIEEPIDRD
                     ALEYYTIAKNLNEHHRFSIDGENPTIRRSPGYPFFLAFFMKIVGTNPNHLYTVQALIN
                     ILTIWIVYLALKQIGANATIRIIAILLFTFNPSFIYVNTFYAEILTMFIMSLLIYLTI
                     NQELFKNKNWNIILQGILIGVLVLIRPTFLYLPIFILVVSILIRIFFHNKKMKDALIT
                     AIIAILTLVPWTIRNRIIFNEWIPTESSSGLALWQANLEIENRTVWYSVTDIAKYEQQ
                     RTASAELQSKLRSEYLQKYDINSLVELNQFLEQKAIEIILRHPIRYCILCVNRFLIFW
                     FSPPIGSTTLKAISPVIFVLALLFRYLITILGICGLWQFARRDFPASFVLICLVVYLT
                     ILHSATHAIQRYFLPLIPLIYFALGYFLNNQKSLNRKGRQE"
     gene            24196..25692
                     /locus_tag="PLO58_02500"
     CDS             24196..25692
                     /locus_tag="PLO58_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HQO73690.1"
                     /translation="MADSVIEQAKSGVRWVGIGQVGYRLLSIASSIVLARLLMPDDFG
                     VIALARLVLGFVMLLSAWGIDASIIVEEKRPKQIANVAFWINFGIMLVLGLLVIGISP
                     WVKKFYDTPVLQPILIWMGLGLIFQSFELVPRTILNRDLNYKYLTIINLSIEIFTQLL
                     VILLAIFGCGVWSLVIPQILASPLRAIPYWIKTKWRPSLYIEKADIKQVLSFGKSILG
                     AELIRYLNTNTDYFLIGKLLSKDKLGYYSFAFNLANWPVENIVKVINTVSLPTLAKVQ
                     SDLAEMRRLFLRMTEMVSLVTFPIFGVLVGITYELLVVIFGTKWIPAVRPLQVIVIYG
                     IMRSLFSPAGRIFLIQKKTHYMFFINLAQFPFLVVAVWLGIRKAEIIGAAVGVTIVLS
                     IGGLITIIVVSKMLKMKITEFIRTISPGFLCCVIFLICGWFFKHLLFGLGLPMVLILL
                     FYLLFAGSLYALSLRLFYPRLFIDFVRTSISIIGITPRNVFEKLRGNQ"
     gene            25695..26903
                     /locus_tag="PLO58_02505"
     CDS             25695..26903
                     /locus_tag="PLO58_02505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQO73691.1"
                     /translation="MTPGTNIVYLCANPGLDLDKMLGPKIHIQAILHGLKTQNINPVL
                     VAVQKSGSVQDYHEFEKVILPHRYLRGFVHRVIPYTGIVDSLRIFLKIIALNRTKHFT
                     LIHERYTGLSWGGVLAAKFLRIPLVLQMVGPGIEEKTLQLNPLKPSRRWLALLNQKYL
                     FSNCDHLILVSKLIASFIYQKRGWILPKHSVILFGPDLLDPLSEAKKTTIRRQIGAED
                     RPLFIYSGSLYRWYGTLDLVKAFHLALQKRPDLKLIIAGSGDDENTIREYIQKKSLTD
                     SVLLIGVVPHAELLKIIQAVDYCLHFYPGEPTYFGSSVKVIEYMAAGKPVISTPQMIE
                     IIEDGVTGFMSKTASPEDFAAKIEEVVSKPDLARTVGKNARNLISSHYTWNHYIEKLI
                     KIYKATLNQT"
     gene            26912..27589
                     /locus_tag="PLO58_02510"
     CDS             26912..27589
                     /locus_tag="PLO58_02510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQO73692.1"
                     /translation="MKKSIKIILKYSSQKRDPFYEIAKKYIKPDSIILDIGAGNGSFA
                     KFIGRNDVFMIDGNPETVVNNQKFFPNYQYVHLPDLPFQNNKFDIIHCSHIIEHLTPN
                     DFFLLLKNCDRCLKISGYIIISSPLMWYHFWDDLSHVRPYSPRIFEKYLCEKSSPNST
                     REKISNKYSIIELQYRYHLTEIGADVINIRSSIVNQVYRIVMQFLTRVGFRRIEKTGY
                     TIVLKKS"
     gene            27599..29527
                     /gene="asnB"
                     /locus_tag="PLO58_02515"
     CDS             27599..29527
                     /gene="asnB"
                     /locus_tag="PLO58_02515"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQO73693.1"
                     /translation="MCGICGIYHHGKIEVSPAEICRMRDFMIERGPDSSGYELLPEIA
                     LGHRRLKIIDLSDAATQPMTNADRSIWTVFNGAIYNYRELRQELLAVGYHFKSNSDTE
                     VLLQGYAEWGTDLFQKITGMFAIAIYDSQKKQLILARDRVGKKPLYFTEQSGIVYFAS
                     DIKAVVAVLPAQPPVSLTALDCYLHHIAIPDQHTIFEGIQKVQPGSYHVFSENGQVVT
                     TYWEWNYAHKVVNDEPIILQNCEQLLIQAVLRRTVGDVPIGTMLSGGVDSSIITAILC
                     RNSTQRIKTFTMGFKNYPMEDILAAREVAKLYDTEHTELILDHNVTENLLELVWQFGE
                     PFADSSAIPTYLISRAARQYITVMLTGDGGDEAFGGYGRTIVPRNAQIFSQIIPPFLH
                     PLVGGILKGIGVNPERNSIIGKILFYINYLKGFPYESFYNTMGFYKYRNSLWSPEYLS
                     KIAGHNPLHPFHENFRKVSDLDPIDQVLFTDGMTRLVYDYLVKIDRATMANSIECRSP
                     FLDTALLEYVAKIPPKMKFKNHQTKYLLKKIAEKYLPHKLIYTEKRGFGVPVDRWIQN
                     DLSGIFETVVFNRYARQRNYFNYDFINKIWNGQRTNVQNHKHRLWALFWLELWHLMFI
                     DKIISRDMPLREVERILK"
     gene            29524..30681
                     /locus_tag="PLO58_02520"
     CDS             29524..30681
                     /locus_tag="PLO58_02520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO73694.1"
                     /translation="MKQINVLHIIGSLQLGGAEKSLLLLLQNIDRAKFNPVVVTMYSD
                     GLNDFFSDDFRKLNIPLYHLHLKSWRDIETFRGLRQIIRRHRIDIAHSHYGALEFFGT
                     LYARLAGVRHCVYTKHNLRFKTSLLFKLGRILLNLILAERILSISKTVTEHLVNTEYA
                     SRSRIRLVYNPVENPKIPNAAHQASIKQKWQIPTGQFIIGNTSRFDSSKGFDIFYLVL
                     KYLRKMGIPVHAVVMGNESSQMEHQRLISENDLHELVTILPYQKDLSPVYSILDCYLF
                     PSIHTEGFGIALLEAMSYGLPVVGLNVGVMNEIIRNQSNGLLPYPEKWEKTFHGNRTD
                     AAKALAKAIAELYNSPVLLRNISANAKVTAEKYSVTNFVNKLEAVYLELFK"
     gene            30678..31826
                     /locus_tag="PLO58_02525"
     CDS             30678..31826
                     /locus_tag="PLO58_02525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO73695.1"
                     /translation="MKILVLTDLFPTLSNPNHGIFIYQWAYYLAQSCDLTVYQIVFKE
                     NQQSVSELELKRFRENYDANQRFAWTQRIKTIGRFDRIWRRSRQFYRQSKLDLSQTIG
                     EFDIIIGQMGCPGGYAAVRLAKKFGKKSIVGLRGSDVNAYLSRPVLKHYMHWTLKNAD
                     ILVTVSEAMKVELIKRGYPESKINVIYNGVDEKLFHPLDQVEAQKKLKLPPNYKYLLF
                     VGTLSQRKGINLLLSAFAELNRPEVKLILIGQGNDRHLIENFRIEHSLQDRILLSGVI
                     PHAQLPLWYAASDVVVLPSLSEGVPNVILEALAIGKPVVASQVGGIPEVLAGNKAAFL
                     CQPGDLNSLFEAIKKALNYSSKIEPFHKFTWEMNTKQFLQIFQEVIRR"
     gene            31823..32995
                     /locus_tag="PLO58_02530"
     CDS             31823..32995
                     /locus_tag="PLO58_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQO73696.1"
                     /translation="MKSNSTQIIFYFCFEGFSRLKDINNHIRETVSWLAKFGYEVHFF
                     NPNIVKPSFSSKVHIHLIPVINYPFLRWATFEFLSFFILTWNALKRRPTHLYFRETSS
                     FTPLLVSKLCHIPLTIEVNGWVLEELRQIGYSRWKLAYIRLNQRLNFVHCNRIIPVTE
                     GLKNLIVTNYPVKSAKIIPIANGTNPEVFRPMPKSEALAKTGLPNTPTVGFIGSCYHY
                     HGVQYLIKAAPLVLQKMPQVRFVVAGDGAQLEAWKTLSRELKVNEAFTFTGKVPFSLA
                     PYYINSFDICVSPWDIEFFSKISGSPMKFFDYLACGKPVIASPVSSVVKLINKFRCGI
                     TCDVKNSEVFAALIIELLSNPKIREPLEKTAREVVTENFTWEITSRKIEQVLKGIR"
     gene            32995..34338
                     /locus_tag="PLO58_02535"
     CDS             32995..34338
                     /locus_tag="PLO58_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73697.1"
                     /translation="MYDQFVRDFYFPLTQHIKGEKIAQYLKILRYNEHLPLEKLQKIQ
                     LAKLYHILNVAYQNVPYYQAIFNQLGIKPEDISSPQDFAHLPLLNKKDIRENLSRLTN
                     PNYSGKIYSGKTSGSTGIPLKFYITNEYSSWDWASRWRARNWFGVQIGDPEVAIWGRP
                     LHSTIRKVIDALKGRIRNTLLISGFEYSEEMLEKLSRQIQRFNPCYFYGYSNSIYRLA
                     LYYKENNLPLPGRLKAIFVTAETLFPQERKIVESVFHVPVSNEYGCSEIGGFAYECPA
                     GNWHVSLENVYLEFIENENGVREIVGTSLTNEYMPFIRYRIGDVGEWINGKCPCGCNL
                     PLMKLHIGKATDIVVMKNGQTFSSEIFDYLNLALLDLNRQPFDQYQIIQTRPSTFTIR
                     YVKSHNYYDSDLDLFQKLLCKTVHDDNIQIEFRPVTAIKPDQTGKMRYFISELKP"
BASE COUNT         9993 a         7645 c         6597 g         9837 t
ORIGIN      
        1 cagtgttgag tgtgaggaac ttcctccagt cgctctttca taatcagttc tccacattca
       61 atacaaacgc cgtattcgcc agtcttaatt ctttcaagtg ccgagtttag atgcgataaa
      121 aatttatttt cacgcgctaa ccagagaaag gctttttcgc gctcctggga gtcggttccg
      181 acgtcagcca tatgataggc ataagcagaa tccattgccg tattgtcatt gcgagtattg
      241 tcgctaacgg tagtggcttc cagccgttcg atggctttaa gagtttcttc tcttttcttt
      301 aagatgagat tcttgaaata ttcaagatcc tctttactcc atgctttttt cgtcgttttg
      361 gcttccatat ttattttctc ctactgctcg aaatttactt aattcgtgtc aaagaaacac
      421 gaactttgtt attttcgatt tttagctctt tttcatactc gccgggcgca aaattatcta
      481 caactttttg cgctagggtc tcattacaga aatactcgat ataattatga acagcggcgg
      541 taaaatcggc tggcgcttca aagttgacct gaatgcgatc ctctacctga aaatcggctt
      601 ccttgcgcat cgtttgcaca tgccggataa aatcgcgcac caagccttcc tgtaaaagag
      661 acggagtcaa tttcgtatcg aggataacag tgattccgtt actttcggct atggcgaagc
      721 cttcattttc cttgcgttcg acgatgatct catcgcgtga tagccaaatt tttccatcaa
      781 ttaaattcaa ctcgaggttt tcactcttct caaggcgtcg ggcgacttcg atcggattca
      841 gctcggcaat tgcctgccga attgctggta aacgctggcc gtattttgct ccgagctgag
      901 cgtaatttgg tttgatttga atattaacaa actgcgtttc gtcggtgata aaatctaaag
      961 cttttacatt taattcttcc aggatttgat ctgttaaatc taccagatca ttggcggtaa
     1021 cctcagcagg caacttcacc cacatttgcg ctaaaggctg acgcactttt aggttggctt
     1081 tgttacgcgc cgcccgacca actgacacga tttcaataat ggtgtcaatt tttttcagta
     1141 atgcctcgtc gaactcctcc tcagcctgcg gaaacttagc caaatgaatg ctttcgggag
     1201 catccggctc cagacctcga acgagattct ggtaaatttt ttccgttata aacggcacaa
     1261 cgggcgccag gattaagatc aatcctttca atgctttata taaggtctga taagcgctcc
     1321 acttatcctg gtcattgtcc gatttccaaa aacggcgacg actgcgccga atataccaat
     1381 tggaaagcac ctccagaaaa tcatctacgc ctttcataac gcgagcgacg ttataatcgt
     1441 ccagtgcttc ccgtgacagg cgaatcaact gcaccaaccg cgccatcaac caacgatcca
     1501 gttcgcttag attttcatcc gatacctgat gcttttgagg cgaatatcca tccaattcgg
     1561 cataagttgc aaaaaaggaa taggaattcc agagtgtaat caggcgcttg cgataatcct
     1621 gcgcgatatt ataaccgaaa tttaaattat taaacggatt atgatcggca tataaccagc
     1681 gcatcacatc cacgcccatt ttttccgcgg cttcatcaaa ccagatggca ttgccggcac
     1741 ttttgtgcat atcttggcct ttttcgtcct tgactaaagc atagccgaga atcgttttga
     1801 acggctcccg attttccaat acagtgctca tcgccagtag agaataaaac cagtttcgga
     1861 attgacccgg caaggattca cagataaaat ctgccgggaa ccactcttcc caataagccc
     1921 gatcagtagt gtaatgcaga gtagaaaatg gtactattcc agcatccaac cagggatttc
     1981 caacatccgg aatacgactg actttggcac cacagcgtgg acaggtaatc tgaacattat
     2041 caacccaagg acgatgcggc gagtgcccct cgaatttatc ccagccttcc atggcttttt
     2101 ctttcaattc ctcgcgacta ccgataacgg taacctgacc gcaatcttga cattcataga
     2161 tcggcaaagc cagtccccag taccgcttct tggaaatcat ccagtcgtgc atattacgca
     2221 accagtccag ttcatgttgt aaaccatatt caggaatcca acggattttg cgagttatat
     2281 ccatcattgg ctgacgcacg ccatccatag cgataaacca ttcatccacc aatcggaaaa
     2341 tcagctcggt gccgtgccgc cagcaaaccg ggtaacggtg cagatatgaa tcggtacgat
     2401 atttaatacc tttggcttcc aggtctttaa aaatatccct gctaatgcca tctacattgc
     2461 ggccagttag ccagccaaag ccttctaaat aattaccgaa ttcatcgagc ggagccagaa
     2521 ccgctaggtc gtgaatttta ccaagctcgt gatcttcctg accgcatccg ggagcaatat
     2581 gcacaattcc cgttccttcg ctggcactga tctcttccca atcaattacg acatgcttga
     2641 cattcttctg agcgggtaaa tcatcaaacg gaccgtggta agtccagccc agcatctccc
     2701 gaccgggaat ttttccttca atggtatatt cgcctttgat tacatccctt tcacgtcctt
     2761 cgacaacata gagaatttca ttgccctgcc gaattttcaa gtaattcaaa tccggatgaa
     2821 cggcggcggc agtgttcgag gtcaacgtcc agggcgtagt cgtccagact aaaagcgatt
     2881 cgccaggacg attgattagc ggaaatttga ccgtaatact cggatgtgtt atttccttgt
     2941 aaccttccgt agcgatttca tgttccgata gcgccgcacc gcaacttgtg caccagggca
     3001 taacgtcgtg ccctttataa atccaaccat tttcatggca ttttttcaga aaaagccaga
     3061 ttgtataatt gttttcatcg gacattgtat aataggaatt cttccagtcc atccaataac
     3121 ccagccgaat tgactgctcg gtctggattt tcgaatattt tcgcactcgc tctttacaac
     3181 gctcgacgaa tttctcgatg ccatatttct cgatatcacg ttttgattta aaaccgagtt
     3241 ctttttcgac ttcgacctcg acccataaac cctgacaatc aaaaccgttt tgatagcgag
     3301 tgcggaagcc attcatcgct ttataacgat gaaaaatgtc tttgtaagtg cgcccccagg
     3361 catgatgaac acccatcgga ttattggcag ttatcggtcc atcaataaat gaccatttcg
     3421 gaccattagc attctgcgcc actaattttt taaatatttg attctgttcc caaaacttca
     3481 ggatttcatg ctcgatagct acaaaatcta cactggacgg gactttcttt atcactgaaa
     3541 ataaacctcc taacgccgaa acaaattgaa caaaatgaaa aatattagac taatcagcag
     3601 acaggtaacc cacggaaaat aaaaagtaaa atgttcccgg cgaattataa tatcgcccgg
     3661 caaacgacca atcttatcaa gaaatggaaa ttttccggca actgtcaaaa gaactccgac
     3721 tacaatcaac accactccga atattattaa caatttgcct acttgattca tttaattgct
     3781 accaatctcc attcctgatc tggtttaccg ctagcacttt ccaattaacc gcgaaattta
     3841 gatttaactg gctaaaaaaa caattaaaat ggctcacttc ctatttgaaa aaagaagggc
     3901 ttccgcaagg gaaacccttc ttttcggaga gagaaaattg gagagttatt tactaaggtc
     3961 tatagtctca aaaaaggtaa cattcttacg tttaatcaga atcagtacga tgtctgtttt
     4021 cacctcttcg agggctttct gatactcagc cacgttgctt atatcccgtt ccccaatcct
     4081 tttaataata tcaccaacct gcaaaattcc agcggctcta ccattccggt caaccccagt
     4141 aaccacgact ccggtttcat caggactgat accgtattgg ctggcggtga ctgaattgat
     4201 ctcgtcgacg cggataccga gtttggaatc agattttgta tcggggtaaa caccgcgctc
     4261 gacagattca ggtaattcgc cgagtttgac atcaattttt aactcttttt taccacgcag
     4321 aactactagg gttttgggct taccaacttc ggaagtggaa accagcgtgc gcaggtgcgc
     4381 cgaatcctta attgtttttc catcgaattc cttaataacg tcgccgactt tcaaaccggc
     4441 tttctccgcc ggactgtctt taacaacttc gctgatgata gccccctgac gactttcgat
     4501 accgagcgat ttggcaatat tttcatccac atctccgatc accactccca gccaggctcg
     4561 aacgactttg cctttttcta ttaaatcagt cataactttc ttagcaagat taatcggtat
     4621 ggcaaatcca attcccacat taccttgtga cattccggcc gtagcaatag cggtattaat
     4681 gccaatcaat tcagcccgaa tattgaccaa agcacctcca ctattgccgg gattaatagc
     4741 cgcatcagtc tgaataaaag attcataatt agtgttatta atcactccgc tacggccttt
     4801 agcgctgacg attccagccg tcaccgtatg agccaggttc tcgctgaaag gtgatccgat
     4861 tgcgagaacc cactcgccga cgcgtagagc atccgaatcc ccgagaatcg ccggttgtaa
     4921 atccttagcg tcaatcttta tgacagccag atcggtgaga ggatctgtgc caacgatttc
     4981 agctttaaat tcacgattat caattaattt aacactgatt ttttcgcctc gttcaactac
     5041 gtgattgttg gtcaaaatat aacccttttg gtcaacgatt actcccgagc caagcgccct
     5101 acttcgcatc tcgccttgtg gaaatccaaa aaaacgccag aatagatcat catccggaaa
     5161 acccggaaaa tcccgacgca gattagtttt gatgactttt tcggtcgtaa tggtcaccac
     5221 tgatggagta actgattccg caatctgcac aaaagcatcg ttaaaagctt gcagagaggg
     5281 aatatcctca ccagccgtta ccgccgccgg ccgaaacgtc gtcattccgg aattcttgtc
     5341 ttcataaaat acaaccttgc caggtctaat aatgatgctg gcaatggtaa atccaagggc
     5401 aaccagtaaa attccgccta ttactatacc aatttttcgt ttcatatctt atcccctttt
     5461 taatttattt ttaaaaaact ggtagattaa actaatcttt ttttttattg tttcaaatac
     5521 taaacaagag aatgtattgg ttatttgcat ggatgtatga ttaaatacat tattaaagtt
     5581 cgacgagaaa tttagaaagt gctgttaagt ccttataagt gcttttaaat gctactaagt
     5641 cctcaaaaga gcttagaagt acttaaaaga taacttnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnctac cgagctaagc gaattcctga cccattttgg acgaaacagc
     5821 cgtttcctgc gcggcgcctt tcaccgtttt ttcgtgcctg aactgctgga atttttcaag
     5881 cagttgggaa ttccaaccgt tactgaacgt ggcggtcgta ttttcccagt ccgcaatgac
     5941 gctaatgaaa tcgttacggc tttagtcaat tgggcaaact ctaccggcgt tatcattcgc
     6001 ccgaatactc gcgccaacca gctcgtcctt accgaaaatc gaattagcgg cgtaatcgtt
     6061 aggaatccaa attcaactac tgagaaaata aatgccgacg cagtaataat tgccactgga
     6121 ggagcgtcct atcccggcac cggttccact ggcgacggct atcgtctcgc taaatctgca
     6181 ggtcattcca tcacaccggt tcgaccggcc ttggtgccat tggtcaccgt gggcaatctt
     6241 gccgcctgtt tacagggact gagtctaaaa aacgtaggta ttaaagtatt cgccgacagc
     6301 agaaaaattg ccgatgaatt cggcgagatg ttatttaccc acttcggact ctcgggtccg
     6361 gtaattttga ctattagcaa actggtggtt gatgccctaa atgccaaaca aaaggtgcgg
     6421 attagtattg atttgaaacc ggcactcgac gaccaaaaac tcgacttacg cttacaacgt
     6481 gattttgctc agcatggtaa aatgcaattt aaaagtgtcc taaaagagtt cctcccccag
     6541 aaacttattc cggtttgcct tgacctaata aaaattccac ctgaaaaaac ttgcaatcaa
     6601 attaccacgg ccgaacgtcg tcgtctccga ctctggctga aaaactttaa cttcgacatc
     6661 agcgggcacc gcggttttgg cgaagcgata attaccgctg gtggggttgc cctgaatgaa
     6721 gttgacagtc aaactctgca atctaaaatt gttcagggac tttattttgc cggtgaaatt
     6781 ctggacttag acgctgacac cggcggctat aatcttcaag ccgctttttc aaccggatgg
     6841 ctggcgggac aatctgctgg acgttaatta ctccgtgatc attaaaaatt atccgtacag
     6901 aagagtcgaa cttgttattt aaggaagaat tgctttaatc tcaccagttt ttcctcgatg
     6961 cgatgtggct tatttatact tggaaaaaat atccaaaata tatcgcattt ttcgtggttg
     7021 caaagtttcg aataggattt tcagttcaat cgttggaaat ttagtttaat gtttttgccc
     7081 actactaaac aggaaatgga agaactcggg tggcaggagc tagatgtaat tctggttact
     7141 ggcgatgctt acattgatag cccgcacatt ggcattgcgg taattggaaa agttttactt
     7201 aaagctggtt atcgagtcgg tgtcattgcc cagcccgccc tgaatgttcc tgacgacatt
     7261 atgcgcctcg gcgaaccgtg tctcttctgg ggtgtgaccg gtggttcgat tgattcgatg
     7321 gttgccaatt acaccgcttc tttgaaaaaa cgccgccatg acgattatac tcccggcgga
     7381 attaacgacc gccgtcctga tcgggcggta attgtttaca ccaatctcat tcgtcagcat
     7441 tttaaaaaaa ccaaaccgat cgtgctgggc ggcatcgaag ctagcctcag acggatagcg
     7501 cattatgatt actggagtga ctccattcgc cgctcgattc ttttcgatgc caaagctgat
     7561 tttatcactt acggtatgag tgaacgctcc attctggaat tggcggaaag tcttcagaaa
     7621 ggttccgatt atcgcgacat tcgcggtatc tgttatattg ctcctcacaa accggaaaat
     7681 actttggaac taccgtctta tgaaaaggtg cgtgacaatc ccaaaacttt tacccgtatg
     7741 tttcatgaat tttatggaaa taatgacgcc agaagtgctc agcggctttg ccagaaatac
     7801 gccacacgct ggttagtaca taatccaccg tcattgccgc tttcgcaggc tgaactggat
     7861 gaagtttaca atcttaatta cgagcgtgat gtgcatcctt attatgccaa acaaggctca
     7921 gtcaaagctc tggaaacgat tcagtttgct gttaccaccc atcgcggctg ttacggcgaa
     7981 tgcaatttct gcgcaattgc tattcacgaa gggcgcacgg tacaatggcg cagtccggaa
     8041 tcggtcacca atgaagtccg gagttttgcg acaatgccca atttcaaggg aatcgtcgcc
     8101 gatgttggcg gtccgactgc caatatgtac ggcttcgaat gtccccaaaa attgaagacc
     8161 ggcggctgta aacaaaaacg ctgtatttat cccgaaatct gtcctcaact accagttagt
     8221 cataagaaac aaatcgaatt gctcattaaa ttgcgtgcct taccgggcgt tcgcaaagtc
     8281 ttcgttgcct cgggaatccg gcatgattta gtcctagctg ataaaaaatg gggccgaaaa
     8341 tatctacgtg aagtcatctc cgaacatact tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nntccaatta actgtgaacc ggatgatgaa ttatccgcgc tcggtcgcga
     8521 gattatccgg ttacaaaaag aatggcaaga attgtctctc ctcattggtt tgaccgccca
     8581 gatcaacgcc ggcttgaatc tggaagaagt acttgaccgc atctttgatt catttcacgc
     8641 cattattcct tatgaccgca tcggagtggc tctactaact gatgacggca aaaatgtccg
     8701 atcttgttgt actcgtagtc gtctcccagt tattaaatta aaagaaggtt actctgcccg
     8761 cttgaaaaat agtagcctcg aacagatcat tcgcaccggt cagccacgca ttatcaacga
     8821 tcttgaagaa tatttagcgc aaaaaccacg atcaacctct acgcgtctga ttttatccga
     8881 aggaatgctc tccagtctta cctgtccgtt gattattcag acaaaaccgg tcggctttat
     8941 atttttttcc agctgtaata aaaataccta tcgcgatgcc catatcgaac tttttcagaa
     9001 aatcgccgac caggtttcgg ttattattga aaagagtcgt ctttatcaga atctagcggc
     9061 tttaaacgag gaaaaaacaa aatttctcgg catcgccgcg catgacttac gcagtcccat
     9121 cggagtcatt aaaagttata cagatatatt actggccggg ctcgtcggaa atttgactga
     9181 ccaacagacc aatatcatca aaacgatcga aaataactgc actcgtatgc tcaatctaat
     9241 caatgacttg ctggacatta gcgcgatcga atccggtaaa ctgacccttc aaaaaacaac
     9301 aatcgagccg agcatttatt ttactaatta tgctgaaaac gctatgcgtt tagcggcggt
     9361 caagtcaatc catttcacaa cattaatcgc cccacaaatt ccgccgatcg aatttgatcc
     9421 caaccgcatc acccaagttt tagataatct cgtctcgaat gctatcaagt tttcttcacc
     9481 agaaacgaca attactttgg cggtcaaagc tataggtaaa aatttagaaa tcagggtagc
     9541 tgatcagggg cagggaattc cagccgcgga aattcctaaa ctcttcaaac cgttcagccg
     9601 gaccagtgtt cagccaaccg gcggtgaaaa aagcaccgga ttaggcttag caattgtccg
     9661 ccgcatcgtg caagcccatg gcggcaaaat tcgagttaaa agtcaacctg gtcagggaac
     9721 cgaattcatt atccacctac catttatctc taagtctgat aattaaactt ttaatatcat
     9781 caccgcatct tagcgatata acaatccaat aatcctctat cgtaagcaat tgaaaattaa
     9841 aggttactca aaattacata ttggacaatc ccaagaaaat ttcagatttt aaacggccaa
     9901 tattttttat tccatacctt aaataatatt aatatatttt gaccattgaa ataaggaaga
     9961 actatgagag aattgaaatt taaaccgggt gacattatta ttaatgaagg ggcatacggc
    10021 tcggaagctt atatccttaa agccggttca gtcgaggtat ctaaaatgac cgatcacaaa
    10081 aaagtcattc ttgctatttt acaaccacaa gaaattttcg gcgaacttgg tttaattgac
    10141 gacaaaccac gttcggctac tgtcatcgcc aggactgcgg tgactgttga tgaaattaca
    10201 cgcgaggatt ttagcgccct gcttgacgat aaaatgcctt tcgttatccc aattatgcgg
    10261 tcatttttcg aacacctgcg ccaggctaat caactggttc tccagatgga ggcgcaaatt
    10321 acagaagcgt cgaaaactgc ccctcaacca aatgccccga ccgaaattaa actcgagggc
    10381 ttaaccccaa ttgccagtca atctcttaaa aatcgtacgt taatattacg aaaattcccc
    10441 tttaaaattg gccgtgaaag tggacagcgt cgcgaagatg tttttattga taacgacttg
    10501 tccctaattg acaacatccc ctataacgtt tcgcgtaatc atatgtcaat caatttttat
    10561 aatggccaat actacattct cgaccgcggc agtagcctgg gtactattgt caataaaatt
    10621 ccgatcggtg gccgtctttc caatttcaaa gctgaacttc ataagggcga aaataccgtc
    10681 attcttggaa ctagcaattc tcaatttcaa tttaaaatta cgatcgaatg attccccgca
    10741 aaaacctcgc ccgggctttc caaaaggcta tcgaacagcc taactatgca ataaataact
    10801 tcgtccatag attaaaagcg tctttttcat accattttct aaatggcaat agtctcgggc
    10861 cagaaacaat ctcccttttt ctaacctaca aatgcaattt aaaatgcgct atgtgcggtc
    10921 aatggggcac caatggcgcc ttccgcgaat atccaagcga agtggttcgt cagcaacttt
    10981 ccctcgacga aatccagaat ttgattgacg acgttcggca ctacaacccc aacatcacac
    11041 tcttcggcgg tgaaccgatg ctttacccaa attggctgaa agtcgtcgaa atcgtcaaag
    11101 cggccggatt gcgttgcaat atcgttacca atggcactta catcggaaaa tgggtccgtg
    11161 aaattgcgga ttcgaggttg gacgagatta ttttttcact tgatggaccg gctgaaattc
    11221 acgataaaat ccgcggcgtg cccggcacct ttcaaaaagc catcaatggc ttacaagaat
    11281 tgaacacttt aaaaaaaact cgcaacttaa atagaccgct cgtgaatata aattgcacca
    11341 ttaccgaatt gaattatcgc tcgattgatg agattattaa aatcgcggag gacattggcg
    11401 cttgtggatt aactttacac catcttcttt tcctggatcg tgcgacagtt atggaattta
    11461 ttccgttctt tcaggaaaaa tttggccaga cgccgaccga ttggctcggt tttatcgtgg
    11521 acgaactacc gcaaatcgat cccgaatatc taattgccaa acgtaatgag ctaaaaacgc
    11581 gacggtttga taccgacgtt aatttctatc ccaatttaga agataatgaa attcgccaat
    11641 ggtacaccca attccaattc aaagcctctt catacaaaaa tcgctgccaa agtctatggg
    11701 cgacggctta tgtttttccc gatggctctg ttcgtcctta tcactcgatg aattttacac
    11761 ctggtaatat tttacaggat aaattctcaa aaatttggaa caatnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnngaattt tacacctggt aatattttac aggataaatt
    11941 ctcaaaaatt tggaacaatc caagttacat cgcctatcgt aggtttgtaa ggaaaaacaa
    12001 gtgtttttca atctgttcaa aaggctgcac tgaatttttt cgatattgat attgataaag
    12061 tataattaaa ttgactaaga atacaataat aattaaatta acttaaaatg agacaggttt
    12121 atggaaaaaa tacttatagt agatgatgag caggaaatag ctgagttatt agaagaagta
    12181 ctggagcaac tcggttataa aaccgcaatg gctttctcaa aagcacaggc gtccgaagtc
    12241 ctcagagaat ttgaacccag aatcgttcta ctggacattc gtttaccaga cggtgatggc
    12301 atttccttcc tgaaagaaat caaagccagc caaccagccg tagaagtaat catgataacc
    12361 ggattagctg atcgtgatat cgccatagaa gctctgcgcg aaggagccgc cgattacatt
    12421 tgtaaaccaa ttgatcttga ttacctagca aatagtttac tcgctaatat tgtaacccgc
    12481 tacaatacgg acgctccagc actctagaaa tctgagaggt aatgcttatt ctcagcattt
    12541 tttataaaat cgggtaaaaa aatgaaacga aatcaatttt tagaattacc caaaaaaatt
    12601 attttattgg tttgtataac cagcctcttc agttttgctc aaaaacgtga atcgcctatc
    12661 agcactgatt cctaccaaaa tgaaatgacc ctgtcacccg gcgatcgaat aaaagtctct
    12721 tacattgata ttgggcccca aggtgttcct atcgaaaaaa gttcgattct cgaggtgcgg
    12781 cacgacggca ctatttttca tgaattgctt aaagtcgttt atgttcagaa cctgaccatt
    12841 aaacaggtcg aagatttatt gacggtaaaa ttttcggaat tctttaccca accgcaggta
    12901 gtagtcagta tagttgaaaa aactacgatc aaggtttttc tctggggcgg ggttgacaat
    12961 ataggtgtat atcgtatatc gcctggaaca actctcgccg aatttataat tacgcatggt
    13021 ggcttaacca gcgatgccaa catcagcaaa attaccttaa cccgtaaaga tggttctgtc
    13081 atcattttcg atcttgaacg atacttgttc agtaacgatg aaaccaataa tgttgaattg
    13141 catgatggag ataaaatcat cgttccgcga cttataatga aagacgagta cgctcgcctt
    13201 tctaagggat atatcctgca ggctggaaac gttctggaaa tcactatcaa tgaattagca
    13261 actatggaaa aaagtccggc aaccccggaa aattatataa ttgaccagga cggtaatatt
    13321 tatcatcggc gctttggtct agttcaattg ggaggtttaa ctgttgacaa agctcaggct
    13381 actctgaccg aactggcaaa aaaatattat cgtgagccgg tggttacaat agatgttgtc
    13441 aaacttactt ctcgcagtgt ctttgttttt ggagaggtta accgaccggg aatttatccg
    13501 attgaaggta atatacgact ggcggaattt ctggctgata ttggcggttt aacctccaat
    13561 gctgacccac gtaaaattat agttactcgc gaaaactata aaccggttac ttttgatctc
    13621 aatgaatacc ttttcgatcg aaaagatgaa aaaaatatct atcttgaaga tggggatcga
    13681 attatagttt taagtcgtaa acgaggattt attgtcaacc tagcggaaaa aatccaacca
    13741 ttcaccactg ttctctatat tatttcaacc gcacttaata tctatttttt aatcacatat
    13801 attaaataga aatgacctgc ctatggcaca ttatgaatta aatctgcgcg attactggcg
    13861 aataatcaag aagcgaaaga ttatcattat tgttactctt gtattgctca cctgctttag
    13921 cctgattttt gccataatga atcggcctac tcctctttac cgcgctaccg ccagcttgaa
    13981 aatagagaaa agtaccaatt taaccggtct ctatatgtac agtatttcct ggggaactgg
    14041 tgatgacctc gctactcgcg ccgaagaaat aaaaagctac ccaatgatag aaaaagccgc
    14101 ccagaaatta gggctaattg attcatcttt gacttctgag caaatccgca gtaacaaaga
    14161 atacctcgat ttagtatcaa gactcaaatc gcgcgtgaaa accgagcagg aaggttacac
    14221 caatattatc aatataatag taacagatta cgacccagtt ctcgccgcta atctggcaaa
    14281 tgccctggct caggtttacg aagtcgaaag cttcaaggaa aaaaacctcc aggctcaaaa
    14341 agctctggaa gccgtccgta accagcgcgc tatggctgaa caggctttac gtatctccga
    14401 gcaaaaagtc caaaattacc gtgagcaaaa taaactggca actctcgatg gagcggcatc
    14461 acgtttaact gctgaattaa actctgccga aactaatttg gaaaaaatca gaggtgacat
    14521 ccatcaaatc gacaacattt tacgtgaaat tgggaataac cgcgaatata tttatctcgc
    14581 aaacttaaat ctgcttctca gccagcctaa tcgtttcctg gaagatttac aaactcagct
    14641 taatcaaatt cgttcccaga ttaccaatta ctcccaatat tacacccctc aacatccagc
    14701 cattattgag cttcaacagc aattaaaaaa cactgaaaac agatttattc atgaattaga
    14761 ggcgtttcgt caaaacttag cccgagccga aactatcgcc gaagaaaaat ataaaagatt
    14821 aaacgaagaa tataagtctt taccattaat tgaattgacg ttgactaacc tcgagcgcga
    14881 acgtgatgtc aatacccaga tttatcagca gctcgaaatc cgctaccagg aagccataat
    14941 tagatattca gaacaagtca aggaagtctt tatcattcga ccggcttttg tgccgacttc
    15001 acctatcaat cagaccacag tcggcccaac cacagctatc ggtatgatca tcggtttaat
    15061 tctcggcatt gtgctggcat tcgtcgccga gacactggat acgaccttca ataccgttga
    15121 cgatattaaa gaagccctta acaccaacat tatgggaatt attcccttcg ttgattttca
    15181 aggcttaaag tcccaactag ctgaaaaagc cgaaactcca gttcctgatg aaatccttga
    15241 catgcaagcc aggcttgttt cctattatag tcccaagtcg agcattgccg aaagcttccg
    15301 aagcttgcgt accaatgtgc attttgcttt attggataag ggttataaga ctttgatgat
    15361 tacgtcgtca atttctaatg aagggaaaac cagtatagcc gccaatctgg ctatttctat
    15421 ggcccaaatc gggatcaata cccttttggt tgacgccgat ctgcgcaaac cacgtatctc
    15481 gaaactattc ggaattgatc gcgaaccggg cttaaccgat gtaattttgc ggaaagaacc
    15541 gatagacaat gtaattcgca ctatgtcgga tttaatggtt ggaactatgg catgcgatat
    15601 acttcggact gataacattg ccggaattga gtatctgaat attttaacca gtggaaaaat
    15661 agagcgcaat ccagcggaaa tcgctgcctc taagttaatg gacaatttaa tagctgaatt
    15721 aaaaagacgt tatgatctgg taatattcga tacttcgccc cttattccag ccaccgattc
    15781 aacgattctt ggaacaaaag ttgatacagt gttgcttgtc tattatcagg gaaaaatttc
    15841 gcgcggcact ctacgacgtt ccaaaagcca actagaacta cttaaaactg atattctggg
    15901 cgtagtcgtt aatggtatga aagccgaact aagtgccgat tatgccgatt ataaatatgg
    15961 atatgaatat cactatcaag aagaatctgc cagaccgaaa aacaaagtaa ttactgcatt
    16021 agaatctttt ttcttgaaac cagccgtagg aatgaccaac gggtttttca gtcaattaaa
    16081 taaattgagg gctggtctag ctcttattgc gcttggttta atcattggcg gtggtctatg
    16141 ggcatttacc aaatatcagg ggaaaaaagc aaaatcccag gtagaaagca aaataaccga
    16201 aactatcatc ccgcagacaa cttcatccag cgatacctca gtagccagtc atattttatc
    16261 ctctgacact ctaccgaatc tcaccgccca aactgccccc attattccgt cagaattaaa
    16321 acaactccaa aaacaatatg gtatcgagac catttctcaa cagcaggttg accaaaattt
    16381 gactgaaagt ggactactaa ctaataattc tcccacaaca gttcaatcct ctataccacc
    16441 ggctacccaa tctgacaagc aaacagaacc gcttatcagc attgtctatt catcaactat
    16501 tttgtcaaaa actgatccgg ccgcctcagt tcaaccatta ccaagttctc ttccttatac
    16561 tttaataatt gaacaatctt tcgataagtc aactctgatt aatcaattaa ataagcttcg
    16621 acggaacggg ctctcagctt ttattactat agattttagc gatcctaaac aaaaaaatta
    16681 cttactgtgc tatggcagtt tttcatctga aaatgaagca attaacaaat ccaaagaact
    16741 tcaattcctc ggattccgtg gtactttcag aacaataaaa ttaccgttca gcatattgct
    16801 tgatgatttc cccactcttc aatctgccca gcaagctata gaacgtttcg catcaattaa
    16861 agattaccta tatatacaac catccagtca aaaaccggac gccaaaaaga cctattacct
    16921 cctttttggt ggtttcccta gcactgaatc caccgaatta gtaatgaata cctacccgga
    16981 attaggtcac aaaactatcg ttatacgcta aaaagagaaa aaatgccccg tcataaacac
    17041 cataaatcca gaagcaatag aaaggcaatt atttttctgg ttattccaat ttcactaatt
    17101 atcgctataa caatcattat aataattcgt gaaccaaatt ggccattcct cagcatttta
    17161 cgtcaggatg agactgctaa tcaagaaaag tacatgggca gtgccgtacg agtagaaact
    17221 cccattcgac aaaagtacga gactgaatgg cggaatgact actttgagac ctggcaaaaa
    17281 acttacgaaa aaatattaca gaacactgat aaaaacgaga ttaaagaact cgagcgcgag
    17341 ttaaaagcga gcgaaaaagg cattaattag tatgagcctg attgaggcct tcgaaaatcg
    17401 gataaaccgg ttatttatat ttctaagtat acctctttcg atgcttgttg ctctcgtctt
    17461 agtaaaagtt gatccggtat atgctattct agcggcagtc ggtctcgggg tactaattgt
    17521 cacttttacc aatactgatt tcgcgattta tattttaatc ttttcgatgc tattatcacc
    17581 ggaaattggc tcgcgtacaa cttctggtga agggatgact atcagaattg acgacctgat
    17641 tctggcaatc atcattctca cctggttagc aaaaactgcc attaataaag aactgggtct
    17701 ttttcgcaaa acacccctaa ataaaccaat ctggatttat actttagtgt gtatcctttc
    17761 aacaggaatc gggatgatgg ctggaagagt gaatttcctt accggtcttc tcttcgtttt
    17821 aaaatatatc cagtatttta tgatttattt tatggtgatg aatcaaattc gcaccaaaaa
    17881 acaaatcaat aattacctga tttctctgat aatcaccttt accatagtta caattttcgc
    17941 tttgatccaa gccccgagcg gccagcggat taccgctccc ttcgaaggtg aggcaggtga
    18001 accgaatact ctgggcggtt atctcgtttt actaatagct attaacctag gtttagtgtt
    18061 gaatgacgac gctatagcca ataggcatct ccgtcgtggg cttcttttga taacaatgat
    18121 atcgatttat cctctattaa tgacttattc gcggggctcc tggcttgccg ctatagctgt
    18181 tatcatcgct tatattgcga tcagtaaaaa gcgctggatt atttttggag ttgtgaccat
    18241 agtttttata ctggctcctt atattttccc tcaacctgta attgaccgcg ttaaatatac
    18301 attcaccgaa caacagggat acgacgctac tctccaggaa catatcggcg gtttaacgat
    18361 tgatacttcc gcgtccgagc gaattcgcag ctggcgggcg gcttttagag atattaccaa
    18421 acgtccggtg ctcggttttg ggattaccgg ctggcgtttt ctggatgctc aatatattcg
    18481 tgtactcatt gaaacaggaa taatcggttt agcggctttt gtctatttgc agttttcact
    18541 attatctaat attcgtaaag tctattccga tgccaagact ccgctatacc gagcgttagc
    18601 ccaaggcttt tttgtcggta tgattggaat gatggctcat agcatcactg ccaatacttt
    18661 catcatcgtg cgtataatcg aacccttctg gttggcgtgt ggtctggtta tggcagttcc
    18721 cgaagtggaa gaattagaaa cagaaaaatt accggaaatc gaaaaaagtc ccacaaccat
    18781 aaatttggaa aatagatagt tatcatggga aaaattgact tggattttaa gcctaccacc
    18841 cgaccagatt taccatttcg ggaattaaaa gacggcggtg ttgtgtatga atatcgtacc
    18901 gatactctcc attcactgaa tgcaactgcg gcttacatct gggtcctatg cgatggacaa
    18961 cataccgttg ctgaaataat cacttcgata aagcagaact ataatcaatt tgaaactgat
    19021 cccaccagcg aagtgatcaa aattatcgaa aaatttagga ctcttaattt attgactaat
    19081 gactgatgcc actatttaaa attcaggttg gctccgctga gatcagtctc gaatgttgcc
    19141 aatcaatcat tagtgaagtt acctcttctg ttttttcggc aatcctaaca ccttcccaaa
    19201 tttccgctaa aatccactat cggattgaga ccaagaacag taaatttctc ctctttcgca
    19261 ataatcgcct gacccaccgt aaccgccaat tatctcgaat tatctatgct ctggaatggc
    19321 aaatagttca agaccttctc actaaaaata aatccgtcct taaatttcac ggcgcggctc
    19381 tcaagcacct tgataccggc tttatctttt taggtgcggc ttccagcggc aagaccagcc
    19441 ttgcgatcct gcttatgcgc tatggctggg agttcttttc ggatgaattc gcccttgttg
    19501 attgcgaccg gcaaataatc ccttttcccc gtaatttgat tatcaagccg catttgcgtg
    19561 ataaaattaa tatcccactc gattacccta ttttcaaact cgacgatgaa tccgacggaa
    19621 aaattgacgc ccactatatc tcgccgtctc atttcggaac ggtttattta agtaaaattc
    19681 cattaaaggg actagtcttt ctggaaaaag ccgaccgcgc tggttttgac ctgcaaccgc
    19741 tggagcatca caaggctttt cggctcttaa cccaacatct tctcaatcgc aactttagcg
    19801 gtgaaaaatg gctcgacttt ttggtaaatt tactgggttc aagtgccgtt tatcgcctga
    19861 aaatttccag cccgatggat ttgacaccgt ccgatagccg agcacttgtt gaacaattaa
    19921 accaaattga atgaaatgat caagtatttt cgcgatttac tcagtgaccg gattcgcatc
    19981 gaaaatttcc ggcaagctat agaatccact gtaaatcata attctattgt agtagaaatt
    20041 ggaagtgctc tcggtactta ttcatttttt gccgcaaaag ccggtgcaaa agccgtatac
    20101 gccatcgaaa tgaatgacat tttctatgtt ggtcgcgaac tcgctgaacg caacaattta
    20161 actgatcgca taaaatttat tcacggccga tcaactgaaa tagatttgcc ggaaaaagcc
    20221 gatctgatta ttatagagga tttcggacca tttttttact atcacaatct cctcgaagtc
    20281 atacgcgatg cccgagagcg ttttttgaaa gcagacggtc gtttcctacc tgcccgaatc
    20341 actttataca tcgctccttt tgaaagccaa gcctggcgcg acgagcttga cctttttgct
    20401 gcggataatg accaactgtt tggtttcgat tggagttata ccaccgaaat agcctttaat
    20461 cagacttatt atgccgattc tcatcccaaa aagcttctat ccgcaccctt tctcctcaaa
    20521 acaatagatt tgaaaaacga aactaatttc ccattccaaa aacaattcga aataaatatt
    20581 aaggaaaatg gtcaaattca cgggctaatc ggctggtggg attgctggtt caccgaccaa
    20641 caatttttct ctaattctcc ccaatccggc aataattctt ggggtcagct gatatttccc
    20701 ttccgttatc ctattaatgt caaatccggc gagtcggtta aaatttccct gatggtactg
    20761 gaatcatcac cgagcggttt aatagattac aagtggaaaa tcacgcatca ttccggcagt
    20821 caagaacaag acactttcag cggtcggttt tttgatctcc aacaattcaa cccaatgcgg
    20881 cgcgatgtta ttcccagttt atatgaaaaa agactgattc aaacctttat cctgaatcaa
    20941 attgacggaa aacgtagctg ggacgagatt gctgaggtcg ttatgaaaat gtttcctgaa
    21001 aaattcgcca gcaaagaaga cgtttttaaa atagttctgc cattaagcaa ttttttaaaa
    21061 ggaaactgaa aactaacgat tgaagttttt tagtgacttt ttcacaaaag atactgatat
    21121 taatggcgat aaaatctccg tcacttaaat gtagttgacg gggctgtgcc attaaaccgg
    21181 ggaggtcgaa atgaacttga taattcagat tccatgctat aatgaagaag atacgattga
    21241 aaagaccctc aatgatctcc cccgatccat tcccgggatt gactcaatcg aaattctgat
    21301 tattgacgac ggctgtcaag atcgcacggt cgaaattgct cgccagtggg gagtaaacca
    21361 tttcgttcat tttaatcaga acaaaggact ggcgcgggct ttcgaagccg ggctggaaaa
    21421 agcactggaa ctcggcgccg acatcatcgt caacaccgac gctgataacc agtacaaagg
    21481 ttcggacatc gcgcggcttg ttcagccgat tctcgctaaa aaagccgaga tcgtgatcgg
    21541 cgaccgtcag attgacaaaa ttgaggattt ttcctggctt aaaaaaaagc tcgagaaatt
    21601 cggtagttac gtagtccgca aagtctccca aactaatatt ccagacgtaa ctagtggttt
    21661 tcgcgcctat agccgtgaag ccgcattgca tatgaatatt atctctacct tctcatatac
    21721 gctggaaacc attatcgaag caggacaaaa agacatcccg ataattagtg tacctatcga
    21781 aaccaataaa accgaacgcc cctcgcgcct gttcaaaagt aattggtact atattcggcg
    21841 ttcctttgca acaattgtaa gaatctacac tatgtaccag cctttaagag tctttttcta
    21901 tactggaatc acgagtttcg gcattggtct aattctatct atccgttatt taatttttat
    21961 gattgttggc cagggcaaag gccatattca gtcggtaatc atcgccgccg tctttctcat
    22021 tatcggtttt ttcctgctaa tgattggctt gctagctgat gtcatcgccg cgaatcgcaa
    22081 attgcaggaa gaaactcttt accgcattaa gaaagattca ctgaagtaat ttgaataata
    22141 atacttaatc aacactatat aagctaatca tctcgtcaga aactcgacat tataaatgaa
    22201 aaatcaggaa gcagaaggct tactttcccc ccttatcaag aaaattcgtc tgaaaaaggc
    22261 agcaaaatgt attcaaaata actccacagt tcttgaccta gcttgtggcg ccggatattt
    22321 gcgccaattt ctaccaagtc aatgtaaata ttatggcatt gatcgaataa cacctccttc
    22381 attaaatctt tttaatgcct tcctccaact tgatattctt cagccagaat cgtttgaaac
    22441 tataaaggaa tggcttcctg aacctaaagt tgatgttgtg actatgctag ctttcatcga
    22501 acatattaaa aatccccaat atttactgaa acaaagtcta ttgttattaa aaccaaatgg
    22561 tttattgatt attacgactc ctcatccaat agggagaaaa ttgcacgatt cgcttgccaa
    22621 attatatctc tgcagtcttt caggcgctaa ggaacatgaa aatttcttcg gacaacaaga
    22681 tcttaaattt ctgctaaaac agtctggata taaactactt tcttatgaaa gatttctaat
    22741 ggggcttaac caattgatta ttgcgactcc attaaaataa gtttatcata attatcctca
    22801 cattaattac aatgaaatat ttttcacgca tacaaaatct actcgataaa tatgaaatcc
    22861 caattatatt tgcgatcctt ttaattggtt tggttatccg gctggcggtc attatcacaa
    22921 ttgaagaacc tatagaccga gatgctttag aatattatac cattgccaaa aatttgaacg
    22981 aacaccatcg tttttcgatt gacggcgaga accctaccat tagacgctcg cccggctacc
    23041 ctttcttttt agcatttttt atgaaaattg tcggaactaa tccgaaccat ttatacacag
    23101 ttcaagcact tatcaatata ttgacaatct ggattgtata tttagcacta aagcaaatag
    23161 gtgccaatgc gacaatccgg atcattgcca ttttactttt tacttttaac ccatcattca
    23221 tttatgttaa tacattttat gcagaaatat tgacaatgtt tattatgtca ttattaatct
    23281 atttaactat aaatcaagaa ctgttcaaaa acaaaaactg gaatatcata ttacagggca
    23341 tattaattgg ggtattggtt ctaatccgac caacctttct atatctccca atttttattc
    23401 tcgtagttag tatattgatc cgcattttct ttcataataa aaaaatgaaa gatgcgctga
    23461 ttaccgcaat tatcgcaatt cttaccctgg tgccctggac tattcgtaat cggattatct
    23521 tcaatgagtg gattccaacc gaaagttcca gcggattagc actctggcaa gctaacctcg
    23581 aaatcgaaaa ccgaaccgta tggtactccg ttaccgacat cgctaaatat gaacagcaac
    23641 gtacagcatc agccgaatta cagtcaaaac tacgttcgga atacttacaa aaatacgata
    23701 ttaattcttt agttgaatta aatcagtttc tggaacaaaa agccattgaa attatactta
    23761 gacatcctat caggtattgc attttatgtg ttaaccgatt tttaatcttc tggttttcgc
    23821 cacccatcgg ctcgacgact ttaaaagcaa tatcaccagt aattttcgta cttgctttat
    23881 tatttagata ccttataacg attctcggca tttgcggatt atggcaattt gcccggcgcg
    23941 attttcccgc ttcttttgtg ttaatctgtc tggtcgttta cttgactatt ttgcattctg
    24001 cgacacatgc aatccaacgc tactttctac cgcttattcc gctgatatat tttgcccttg
    24061 gatattttct taataaccag aaatcattaa accgcaaagg gcgccaagaa taaatactta
    24121 atatgttttc ttatttaggt aaatatatcc atctttgcgc tcttggcggt gaatggaatc
    24181 cttccggagt aaaacgtggc tgattccgtc atcgagcagg ctaagtccgg cgtccgttgg
    24241 gtcggtatcg ggcaagttgg ttatcgtctt ctctcgatag cctcctcaat tgttctagcg
    24301 cgcttactta tgccggacga tttcggtgtc attgcgctgg cgcggttggt tctgggattt
    24361 gtcatgctgt tgagtgcctg gggaatagat gcctccatca ttgtcgaaga aaagcgacct
    24421 aaacagattg ccaacgttgc tttctggata aattttggca ttatgctggt acttggctta
    24481 ttggttattg gcatttcacc ctgggtaaaa aaattctacg atactccggt gctccaacca
    24541 atcctaatct ggatgggact gggactgatt ttccagtctt tcgaactggt gccgcgaacg
    24601 attcttaatc gtgatctgaa ttataaatat ttgacaataa tcaatctaag tatcgaaatt
    24661 ttcacacagt tactggtaat tctgctggca attttcggtt gtggtgtctg gagtttggtc
    24721 attccacaaa ttttggctag tccgctcaga gccattccat attggataaa gaccaaatgg
    24781 cggccatcac tttatatcga aaaagccgat atcaaacagg tattatcttt tggcaaaagc
    24841 attcttggag ctgagcttat ccgctatttg aacaccaata ccgactactt cctgataggg
    24901 aagctacttt caaaggacaa actcgggtat tactcgttcg cttttaatct cgccaactgg
    24961 ccagttgaga atattgtaaa ggtaatcaat acggtatcgc ttccaacttt agccaaagtc
    25021 cagtcagatt tggctgagat gcgacgctta tttctgcgta tgaccgaaat ggtctcgctg
    25081 gttactttcc ccattttcgg agttctggtt ggtattactt atgaactgct cgtcgtaatt
    25141 ttcggaacaa aatggattcc ggcagtacga ccacttcaag tcatcgtaat ttatgggatt
    25201 atgcgttcgc tcttttcgcc ggctggacgg attttcctga tccagaagaa aacgcactat
    25261 atgtttttca tcaatctcgc tcaatttcca tttctggttg tggcagtctg gctgggcatt
    25321 cggaaggcgg aaattatcgg cgcggcggtt ggtgtaacaa tcgtattgtc aatcggcggt
    25381 cttatcacca taattgtcgt atcaaaaatg ttgaagatga aaatcaccga atttattaga
    25441 acgatctcac cgggttttct ctgctgtgtc atctttctaa tctgtggttg gtttttcaaa
    25501 cacctgcttt tcggattggg gttgccgatg gtcctgatcc tgcttttcta tttattattt
    25561 gcaggtagtt tgtatgcgct gagtctgcgt cttttttacc cacgtctttt tattgatttc
    25621 gtaagaactt caatcagtat cattggaatt acaccgcgta atgtatttga gaaactgaga
    25681 ggcaaccagt aattatgact ccagggacta acatcgttta tctatgcgcc aatcccggcc
    25741 tcgatcttga taaaatgctc gggccgaaaa ttcacattca agccattctg catggtttga
    25801 aaactcagaa tatcaatcct gtcttagttg cggttcaaaa atccggttcg gttcaggact
    25861 atcacgagtt tgaaaaggtt attttgccac accgctatct acgcggattt gttcatcgcg
    25921 ttattcctta cactggaatc gttgacagcc tgcggatttt cttgaaaatc attgcgttaa
    25981 atcgaacaaa acattttact ttaatccacg aacgctatac cggtttgtcc tggggtggag
    26041 ttctggcggc taagtttctt agaatcccgt tggttcttca gatggtcgga ccaggtattg
    26101 aagagaagac tctacaacta aacccactta aaccatccag acgctggttg gctctgctta
    26161 atcaaaaata tctcttttca aattgtgatc atctaatttt agtttcgaaa ctgatcgcca
    26221 gttttattta ccaaaaacgt gggtggatat tacctaagca tagtgttatt ctatttggtc
    26281 cagatctgct agacccactt tctgaggcaa aaaaaacgac tattcgccgt caaattggcg
    26341 ccgaagaccg gccgttgttt atttattccg gcagtctata tcgctggtac ggtactctcg
    26401 atctggttaa ggcttttcat cttgccctgc aaaaacgacc ggatttgaaa ttaatcatcg
    26461 ccggatcagg cgatgatgaa aacacgatca gagagtatat ccagaaaaaa tcccttaccg
    26521 attcagttct tctgataggg gttgtccctc atgccgaact gttaaaaatc attcaggcgg
    26581 ttgattattg tctccatttt tatccgggtg aaccgaccta ttttggttct tcggtcaaag
    26641 ttatcgaata tatggcggct ggtaaaccgg taatttcaac accacaaatg atagaaatta
    26701 tcgaagacgg agtaaccgga tttatgtcaa aaacggcttc gccagaagat ttcgccgcta
    26761 aaattgagga agtggttagt aagcctgatc tggcacggac ggtcggtaaa aatgctcgga
    26821 atttgatttc aagtcattat acatggaatc actatataga aaaattaata aaaatctata
    26881 aagcgaccct aaatcaaacc taaaaagcat tatgaaaaaa tcaattaaaa ttatattaaa
    26941 gtatagttct cagaaacgtg acccatttta cgaaatcgct aaaaaataca tcaaaccaga
    27001 ttcaataatc ctcgatattg gggcaggaaa cggttctttt gctaaattta ttggcagaaa
    27061 tgacgtcttt atgattgacg gaaatcctga aacagttgtc aataaccaaa aatttttccc
    27121 gaactatcag tacgtccact taccggactt acctttccaa aacaacaaat tcgatatcat
    27181 tcattgtagc catataattg aacacctgac ccctaatgat ttcttcttac tactaaaaaa
    27241 ttgcgatcgt tgtctgaaaa ttagtggtta tatcattatt tctagtccgt taatgtggta
    27301 tcatttctgg gatgatttat cccatgttcg gccttattct cccagaatct ttgaaaaata
    27361 cctctgcgaa aaatccagcc ctaactctac ccgagaaaaa atttctaata aatattctat
    27421 aatagaatta caatatcgat accatctgac agagattggc gcagatgtca ttaacattcg
    27481 ctcttcaatt gtcaatcaag tttaccgaat tgttatgcaa tttttaactc gagttggttt
    27541 tagaaggata gaaaaaaccg gttacacaat tgtcctaaag aaatcgtgaa ttttaattat
    27601 gtgtggcatc tgcggaatat atcatcacgg taaaatcgaa gtcagcccgg cggaaatctg
    27661 ccggatgcgc gactttatga tcgagcgtgg tcccgactca tcgggatatg aacttctacc
    27721 ggaaatcgct ctcggtcatc gccgccttaa aatcattgac ctatcggatg ccgccactca
    27781 gccgatgacc aatgcagatc gtagcatctg gacggttttt aacggtgcaa tttataacta
    27841 ccgcgaactc cgccaggaat tactcgcagt cggttaccac tttaaatcca attccgatac
    27901 tgaagtgctt ttgcagggtt acgcggagtg gggaaccgat ttattccaaa aaattaccgg
    27961 catgttcgcc attgctattt acgattctca gaagaaacaa ttaatcctgg ctcgcgatcg
    28021 tgttgggaaa aagccactct acttcaccga acagtccggt attgtctatt tcgcatcgga
    28081 tattaaagcg gtggttgcgg ttttaccggc tcagccaccg gtcagcctga cggctttgga
    28141 ctgctacctg catcatattg ccattcctga tcaacacaca atcttcgaag gaattcaaaa
    28201 agtccagccg ggatcttatc atgtattctc ggaaaatggt caggtcgtca cgacctactg
    28261 ggaatggaat tacgctcaca aagtcgtgaa cgatgaacct ataattttgc agaactgtga
    28321 acaattattg attcaggctg tattgcgccg cacggtcggc gatgttccaa tcggtacgat
    28381 gctcagcggc ggagtggatt cgagcattat caccgctatt ctatgtcgga attccaccca
    28441 gcggatcaaa acattcacga tgggttttaa gaattacccg atggaagaca ttcttgccgc
    28501 gcgggaagtc gccaaattat acgataccga acacaccgaa ctgattttag atcacaatgt
    28561 cactgaaaat ctactggaac tcgtctggca gtttggtgag ccgtttgccg attcttcggc
    28621 gatccccact tatttaattt ccagagctgc ccgccagtac attaccgtaa tgttgactgg
    28681 tgacggtggc gacgaggctt ttggcggtta cggccgaaca attgtgccac gcaatgctca
    28741 aattttttcc caaataattc cacccttcct gcatccactg gtaggcggaa ttttaaaagg
    28801 tatcggagta aatccagagc gtaactcgat tatcggcaag attcttttct acataaatta
    28861 tttaaaaggc tttccgtacg aaagctttta taatacgatg ggattttata aatatcgtaa
    28921 ttctctatgg agtccggaat atttgtcaaa aattgctgga cataatccgc ttcacccatt
    28981 tcacgaaaat ttccgaaaag taagtgacct cgatcccatt gatcaggtgc tgtttacgga
    29041 cggtatgacc cggctggttt acgattattt agtaaagata gaccgggcga ccatggcaaa
    29101 ctctatcgaa tgccgctcac cctttctcga cacggcttta ctggaatatg tcgccaaaat
    29161 cccgcccaaa atgaaattta aaaaccacca gaccaaatac ctgttgaaga aaatcgccga
    29221 aaaatatcta cctcataaac tcatttacac cgagaaacgc ggattcggtg tcccggttga
    29281 ccgctggatt caaaacgact tgagtggaat tttcgaaacg gtcgtattca accgttacgc
    29341 ccggcaaagg aattatttta actatgactt cattaataag atttggaatg gccagcgcac
    29401 taatgtccaa aatcataaac atcgtctttg ggcactcttt tggctggaac tctggcattt
    29461 aatgttcatt gataaaatca ttagtcgcga catgccgctc agggaagtcg aacgaatact
    29521 gaaatgaaac aaatcaacgt actgcatatt atcggaagtt tacagctcgg cggcgccgaa
    29581 aagtcactac ttttgctttt gcaaaatatt gaccgtgcaa aattcaatcc agtggttgtt
    29641 acgatgtatt ccgacggttt gaacgatttt ttctctgacg atttccgcaa acttaatatc
    29701 ccgctctatc acctgcattt aaaatcctgg cgagatattg agacttttcg gggattgcgc
    29761 caaattattc ggaggcaccg catcgacatc gcccattcac actatggcgc actggaattc
    29821 ttcggaacac tttacgcgcg gctggccggc gtccggcatt gtgtttatac caaacataat
    29881 ttgcgtttca agaccagcct tttattcaaa ctcgggagga tattgcttaa ccttattctg
    29941 gcagagcgga ttttatcaat ctcaaaaacc gtcactgaac atttagtaaa taccgaatat
    30001 gcttcccgaa gccggatcag gctcgtttat aatccggtcg aaaatcctaa aataccgaat
    30061 gccgcccatc aggcttcaat taaacagaaa tggcagattc caactggaca atttatcatc
    30121 ggaaatacca gtcgctttga ctcgtccaaa ggtttcgata ttttctacct agtgctgaag
    30181 tatctgcgga aaatgggcat tccggtacat gcagtggtaa tgggcaatga atccagtcag
    30241 atggaacatc aaagacttat cagcgagaat gatcttcatg aattagtaac aatcctgcct
    30301 tatcaaaaag acctgagtcc agtctactcg atcctcgatt gttatctatt tccttcgatt
    30361 cataccgaag gatttgggat agccttacta gaggcaatga gttacggatt gccggtcgtc
    30421 ggattgaatg tgggcgtaat gaacgagatt atccgcaacc agtctaatgg gcttttaccg
    30481 tacccggaaa agtgggagaa gaccttccat ggtaatcgga ccgatgcggc aaaagctctg
    30541 gctaaggcca ttgccgaatt atacaatagc ccagttttac ttcggaatat ttctgcaaac
    30601 gcaaaagtaa cggctgaaaa atattcagtc actaattttg ttaacaaatt agaagcggta
    30661 tatttggaat tattcaagtg aaaattctgg tactcactga tctcttcccg acactctcaa
    30721 atcccaacca cgggattttt atttatcaat gggcatatta tttggcgcaa agttgcgacc
    30781 tgacagttta ccagatcgtt tttaaggaaa atcagcaatc agtcagtgaa ctggaactga
    30841 agcgtttccg tgaaaattac gacgccaatc agcgctttgc ctggactcag cgcataaaaa
    30901 caatcggtcg ctttgatcgt atctggcgac gttcgcggca attttatcgt cagtcaaaat
    30961 tggatttatc ccaaacaatc ggtgaattcg atattatcat cggacaaatg gggtgccccg
    31021 gcggttacgc ggcggtgcga ttagcaaaga aattcggaaa aaagtcaatc gttggtctgc
    31081 gcggttcgga tgtcaatgct tacctgtcgc ggccagtctt aaagcactat atgcactgga
    31141 cattgaagaa cgctgacatc ctggtcacag tttctgaagc tatgaaagta gaactaatta
    31201 aaagaggata tccggaatca aaaattaacg ttatatataa cggtgtggat gaaaagcttt
    31261 tccatccgct tgatcaggta gaagcccaaa aaaaattaaa attgccgcca aattataaat
    31321 acctgctttt tgtcgggacg ttgagccaac gcaaaggaat caacctatta ctttcggcat
    31381 tcgcggaact taatcggcca gaagtaaaat taattttaat cggtcagggc aacgaccgcc
    31441 acctgatcga aaatttccgt attgaacatt cactacagga tagaattctc ctatcgggag
    31501 tcattccgca tgcgcaatta ccactttggt acgccgcgtc ggatgtagta gtactaccga
    31561 gtctttcgga aggcgtcccg aacgtaattc tggaagcact ggcaattggc aagccggtgg
    31621 tagcctcgca ggtcggaggt attccggaag tattagctgg caacaaggca gcttttctat
    31681 gccagccagg cgatttaaat tccctgtttg aggctatcaa aaaggcactc aattattcaa
    31741 gtaaaattga accttttcat aaatttacct gggaaatgaa caccaaacaa tttctacaaa
    31801 tatttcaaga agttatcagg agatgaaatc gaattctacc caaataatct tttacttttg
    31861 ctttgaaggt tttagtcgct taaaagacat caataatcac attcgtgaaa cggtcagctg
    31921 gttggcgaag tttggatacg aagtgcattt ctttaatcca aatatcgtca aaccaagttt
    31981 cagctctaaa gtccatattc atttaatccc ggtcattaat tatccattcc tacgctgggc
    32041 aacctttgag tttttatctt tttttatttt aacctggaat gcacttaaga gacgaccaac
    32101 tcatttatac tttcgcgaaa cgtcatcatt cactccactg cttgtctcaa agctctgcca
    32161 cataccacta actatcgaag tcaacggttg ggtattggaa gaactccgtc aaatcggtta
    32221 ctcgcgctgg aaactcgcct acattcgttt gaatcagcgc ctcaattttg ttcactgcaa
    32281 tcgtatcata ccggttactg agggtttgaa aaacctgatt gttactaatt atccggtcaa
    32341 atccgctaag atcattccca tcgcaaatgg cacgaatccg gaggtctttc ggcctatgcc
    32401 gaagtcggaa gctttagcca aaaccggctt gcccaatacg ccgactgtcg gatttattgg
    32461 aagttgttat cattatcacg gtgtacaata tctaatcaag gctgctccac ttgttctcca
    32521 aaaaatgcca caagttcgct tcgtggttgc tggcgacggt gctcaattag aagcatggaa
    32581 aaccttgagc cgtgaactga aagtaaatga agcttttaca ttcaccggca aagtcccttt
    32641 tagcttggcg ccctattata tcaattcatt tgatatttgt gtttctccct gggacattga
    32701 gtttttcagc aagatcagcg gttcgccgat gaaatttttt gactacttgg catgtggcaa
    32761 accagtcatt gccagcccgg tttccagcgt ggtcaaattg atcaacaaat ttcgttgtgg
    32821 aattacctgc gacgtaaaaa actcagaggt tttcgccgcg ttaattattg agcttttatc
    32881 taatccaaaa atccgtgagc cattggaaaa aactgctcgc gaagttgtca ccgagaattt
    32941 cacttgggaa atcactagtc gtaaaattga gcaagtatta aagggaatta gataatgtat
    33001 gaccagtttg tccgggactt ttatttcccc ttgactcagc acattaaagg tgagaaaatt
    33061 gctcaatatt taaaaattct tagatacaat gaacatttac cgctggaaaa attgcagaaa
    33121 atccagctgg ctaaacttta tcatattctt aacgtagctt atcaaaatgt accctattac
    33181 caagcaatat ttaaccagct tggtatcaag ccagaggaca tatcctcgcc acaggatttc
    33241 gcccatcttc cgttgttaaa taaaaaagat attagggaaa atctcagccg tttaacaaat
    33301 ccaaattatt ccggtaaaat ttattctggg aaaactagtg gctcaacggg tattccattg
    33361 aaattttaca ttactaacga atatagcagt tgggactggg ctagtcggtg gcgagcacgg
    33421 aactggtttg gagttcaaat cggggatccg gaagttgcta tctgggggcg accgctccac
    33481 tcgactattc gcaaagtcat agatgccctg aaaggacgca ttcggaatac actgctaatt
    33541 tcgggctttg aatactcaga agaaatgctg gaaaaattaa gtcgtcagat ccaacgcttt
    33601 aatccgtgct acttttatgg ttattctaac agcatttacc ggcttgcact ctattataaa
    33661 gagaacaacc tgccacttcc tgggcgctta aaagcaattt tcgtaaccgc agaaactcta
    33721 tttccacagg aacgcaaaat agtcgagtca gtttttcacg ttccagtttc taacgaatat
    33781 ggttgttcag aaatcggtgg ttttgcttac gaatgtccgg ctggtaattg gcacgtatct
    33841 ctggaaaacg tatatctcga atttatcgaa aatgaaaacg gagtccgcga aatcgtcgga
    33901 acttcgctga ctaatgaata catgcctttt attcgctatc gcattggtga cgtcggcgaa
    33961 tggattaacg gaaaatgccc atgcggttgc aatctgccac tcatgaaatt gcacatcgga
    34021 aaagccactg atattgttgt aatgaaaaac gggcaaactt tttcgagtga aattttcgat
    34081 tacctgaatc tggctttact cgatctgaat cggcaacctt tcgatcagta ccaaattatc
    34141 caaactcgac catcgacttt tactattcgc tacgtcaaaa gtcacaatta ttacgattcg
    34201 gatttagacc ttttccaaaa attactttgt aaaaccgtcc atgatgataa catccaaatc
    34261 gaatttcgac cagttactgc aataaaacca gatcaaaccg gcaaaatgcg ctatttcata
    34321 tctgagctta aaccgtaatt ataaaataaa ggagaaccaa caatgtcaaa ac
//
LOCUS       DAOMJU010000014        31045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1264_length_31045_cov_24.219458, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000014 DAOMJU010000000
VERSION     DAOMJU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 31045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31045
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_31045_cov_24.219458"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..308)
                     /locus_tag="PLO58_02540"
     CDS             complement(3..308)
                     /locus_tag="PLO58_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73698.1"
                     /translation="MDVWISYLTSHPIIGVALTAALVLFIIMVIARLLKWAVISFIIL
                     ALAVGLTYKIAQPKKIIDRIQKDIESVTENDSEKVTDKLQKSTEEAIEKVKESFKKE"
     gene            complement(325..849)
                     /locus_tag="PLO58_02545"
     CDS             complement(325..849)
                     /locus_tag="PLO58_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQO73699.1"
                     /translation="MTNLELNILGIVSEGATHAYNIEKIIAERGIRERTNVGFSTIYA
                     VLNKMQKIGLLMSNLEPQKGLPSRRIYSITPKGRQIFNEEIFKALSQPQHQPSLFETG
                     LTYSGVLNKEALREALTLYDTELGRQIQNKVHELTALSNTNDQVQRALLTRPLVILQA
                     ERKWIRELMGLLLT"
     gene            complement(911..3763)
                     /locus_tag="PLO58_02550"
     CDS             complement(911..3763)
                     /locus_tag="PLO58_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQO73700.1"
                     /translation="MKVHIPKSIVVAALFTTMIACSTSELTRKIPPTPPSLSQSLELD
                     SEIALDPKITKGKFDNGLTYYIRKNEKPERRAELRLVVNAGSVLEEEDQRGLAHFLEH
                     MAFNGTARFKKQEIINFMELAGMPFGSHLNAYTSFDETVYMLTVPTDRDSLLEKGFQI
                     LADWASQISLEDEEIDKERGVIREEWRLGRGAEMRIQEKEFQVIFWGSLYAERHPIGQ
                     IAVIDTFHYDTLRKFYRDWYRPDLMAVIAVGDFDEDRVKNLIEQNFIGLKSPADAPPR
                     PIPPVPDHTETLFSIQTDPELTRNQVDLLIKLPRTEQKYIRDYRQSIVENLYNSMLNE
                     RLEELTKTAEPPFLGAASQKGRLVRSKDCYSIGAVVKDNGIEEGFTAILTELKRVREF
                     GFTETELKRAKSTALRNMEQIYRERDKIYSRSFASEYIRNFLDNEPVPGIELEYEFYK
                     KYVPEIALPEVNVLAKEWVTDINRVVLVSAPEKPDLKIPTETELREIFEKVERQEVTA
                     YIDEVAESDLVRQLPPPGRILQETHIDSLNLTELILSNGVKVIIKPTDFKNDEILLSA
                     YSPGGTSLVPDSNYVAAISSVPIVTESGLGEFNSVMLSKKLAGKVVSVQPSLAELTEN
                     FSASASPEDLETMFQLIYLYFTAPRKDSTAYLSYLEKLKNSIVNRAADPFAAFSDTIR
                     VTLSQYHFRARPWTIAMLDEIDLNKSYNIFRDRFGEAGDFTFFLIGNVELDSAKQMAM
                     RYLANLPTNNREENWRDVGIRPPNGVVQKAVYKGLEKQSYVQITFSGNFDWSRQNRFE
                     LATVTDVLRIKLREQIREEKGGTYGIYVGSSSKQYPYPEFRLTIQFGCDPERVTELTS
                     VIFQEIENLKSEPLGDEDLAKIRQIDLRSYETNLKENRFWLDNLHFYYFNNENPMAIL
                     HYPQLVEDLTKEMVQNAARRYLPLDNYVEVVLYPEQ"
     gene            complement(3824..4759)
                     /gene="trxB"
                     /locus_tag="PLO58_02555"
     CDS             complement(3824..4759)
                     /gene="trxB"
                     /locus_tag="PLO58_02555"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQO73701.1"
                     /translation="MAKDVVYDVVIIGGGPSGLTAAIYTAREMLSTILLEKSMTGGIP
                     AIVDRIENYPGFPDGISGMELMQNIRAQADRFGTNISEFEDVSKIIPGRQCFEVISDN
                     RRFLAKAVIIATGGVPRNLGIASEQEFLGRGVSYCATCDGPLYRNAELIVVGGGNAAI
                     QEALYLTKFARRVTVIHRRDKLRAQPVLQERAFKNEKIRFIWDSVVSNIFGAEQVEGV
                     EIRNVKSGEKISLPAEGVFIFIGWVPNTEFVRGLVNLDDDGHIITDAEMHTNVPGIIA
                     VGDVRSKPFRQIANAVGDGAVGALSASKYLEGLHN"
     gene            4890..5255
                     /locus_tag="PLO58_02560"
     CDS             4890..5255
                     /locus_tag="PLO58_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HQO73702.1"
                     /translation="MHSEHEIILIGDRVLIDPDEDQNKTPSGLFLPPGVKEKEKVQGG
                     FIVKTGPGFPIPHTDDIEDEPWEEGEDEPHYLPLQAQEGDYAIFLRKSAIEIELESKR
                     YLIVSHSNILLLIRENYLE"
     gene            complement(5362..6234)
                     /locus_tag="PLO58_02565"
     CDS             complement(5362..6234)
                     /locus_tag="PLO58_02565"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HQO73703.1"
                     /translation="MKRKLIILLIFMIAVLSAEDYFTEHYDIPYKGEEELDVNISFGL
                     GTLDLKSHDDPKYLVHSIMTYSQRIYKPIVDYKILGNRGRLKMSTEKYDESNKNDKRN
                     LKSSQVNKELNKWHLEFSQKIPIYIAVDLGLGEGRLDFTDLRVRDLKLHCGLSDVNVI
                     FRDKNQDNMRNMVIETGLGSVNVKGLSYANMERFNVECGLGTTELVFDGSLSQDIRGK
                     IEVGLGSVIIEVPDNVAVEVRSQSSFLSSLNLHGFDRISENLYRSGNWETANKRIYLE
                     IEVGLGSVDINWLD"
     gene            complement(6245..7603)
                     /locus_tag="PLO58_02570"
     CDS             complement(6245..7603)
                     /locus_tag="PLO58_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HQO73704.1"
                     /translation="MKPKKLFLILLMIGSVGASPDKFEFDGKQYVGNIVKTFAVGSKG
                     DLVMERVSGDVTIEGEARRDVMIVEKFKIDAYSEVNARQILNEEKARYQQRGNKVIVT
                     GKEHSRRYSSDFIVKVPLTFNTTVQTSGGDININKVSGLQNLNTSGGDIEISECSGEL
                     KSHTSGGDIKIQQVAGNLSVNTSGGDIEIELFDGELWANTSGGDINLDKIQGNGQVIT
                     SGGDINIVNLRAKTFSAKTSGGDIGADKVEAELTLRTSGGDIIIGQIEGNLEIHTSGG
                     DIEVRQVNKNLKATTSGGNIDIGLIKGYCYARTSGGNIEVDRAYDLEAITSGGDILAK
                     GVVGYIYARTSGGDIEARKLFNSDVKNNAIDLETSGGEIEVILPANLNADIDAEITVT
                     SRRYADVNIHSDFPVQKSHEIKSSRIYIYARGKINQGGSLVRLRTINGNINILKADRE
                     SR"
     gene            complement(7616..8647)
                     /locus_tag="PLO58_02575"
     CDS             complement(7616..8647)
                     /locus_tag="PLO58_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73705.1"
                     /translation="MKHDKHFKELSILYFYNELSESERTDFKHHLDECQVCQAEIERL
                     QIIAKKIDYATEPVPSARRLEALNKQVISKISSESRRPKLVNVKTYFEEMFETITIGL
                     SRPRYQLIAASLILVIGIFIGRVGFRANLPKQPEILANFVNYNYKFTDPEKENIQKAF
                     TNYLLKSGGIEDADLLQSEYRTSQNGLVEVNVRVKEDFAIRGGLDDPTIQNMLMYAAR
                     HNQDAEKRLLALRLLNQIEPNPIVDATFVAAMLHDNNESVRLFAAQLLEKRQMTKQIQ
                     EAFQLVALRDSSASLRKIALENLVNKAEQEVVPVLALVAARDESEEIRQMALLALDKF
                     YDKNDREKR"
     gene            complement(8662..9216)
                     /locus_tag="PLO58_02580"
     CDS             complement(8662..9216)
                     /locus_tag="PLO58_02580"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQO73706.1"
                     /translation="MENEEVELIQNIQSGDKMAFARLMEQYGERVMSVAFRFTRNYQD
                     AEDLYQETFIKVYQNLGKFRFESEFFTWIYRILANQAFNLYNKRKRMTLADPGEDDYL
                     WETIPADAQDEADRVTFSNSIQDQIDAALQQLSAKQRVVFILKHFEGKKIKDIAILLD
                     CTEGTVKRYLFRATHKLQTLLTES"
     gene            complement(9269..>10465)
                     /locus_tag="PLO58_02585"
     CDS             complement(9269..>10465)
                     /locus_tag="PLO58_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQO73707.1"
                     /translation="VRIGFIDTGFFKDHEVFSEILSSGRLIAEYDFINDDDNVSDEEA
                     ADTARNSRQSEHGTAVWSLVGGYNPPDYLGAAYGAEFLLAKTERIDTEIHREEDDFVA
                     AVEWCDYWGADIVSVSLGYRDFADGSEYPFEYLDGRTAITTKAVNWAFERGILCVCCA
                     GNDANNFEDGGLLTPGDAFGALTVGAVDAQGEIASFSSHGPTYDGRIKPDLCAMGVAT
                     YYASNSQGAYRTGSGTSLATPLIAGAAALLLESQPELTPAEIITELKRYADRTQNPDN
                     RYGWGIPDVYQSIVNYQPKSFPKGELSFDEIWVYPNPANSNINFYFIWHKATPATAPA
                     YLQVINLNGQIVFEKRLVSKFGGQKEVINWNLHNQSGARVGSGIYFVCLRSSEYFKIN
                     KFIIVH"
     assembly_gap    10466..10565
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10566..10857)
                     /locus_tag="PLO58_02590"
     CDS             complement(<10566..10857)
                     /locus_tag="PLO58_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73708.1"
                     /translation="MIQRIRNIYVHRFRVFGFLLLILPNIVWAGHPVTIDKFTNQPTI
                     RAWIYLSNEPAYNEYFTQLEYPEKTQRRLAKMGTLAKKTASDLVPGEELRAII"
     gene            complement(10937..11497)
                     /locus_tag="PLO58_02595"
     CDS             complement(10937..11497)
                     /locus_tag="PLO58_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73709.1"
                     /translation="MNKAVCPQIPEGELNFKMILLDNGANVKTWREAVRSAIKDGAER
                     FLLVGRGVPTRRSEKSAGIVVVDQINVSGQNPLVGPNDEEFGTRFPDMTDLYSPKLTE
                     DVLIAGKQAGLVLQKGILLVPVSMTSSTSLEEQLIVQNEIAALSQDVFAGAITVRHAR
                     RQCAGLILFSDIDGKQLGLLLAHLDW"
     gene            complement(11537..11983)
                     /locus_tag="PLO58_02600"
     CDS             complement(11537..11983)
                     /locus_tag="PLO58_02600"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HQO73710.1"
                     /translation="MIKTDEIRDRRFRKVFRGYDPVEVKYFTEMLADEFAQMEEKLEK
                     LEAIVNEFDQTRKGRDKILQEAQDRAASIILDAESIASEVIANAETKKRQIQEAIDQL
                     NLEQNKLIQRLRSVLKNQAEILTWLEEAPDWSEEDNTLNESSEETT"
     gene            complement(11980..12687)
                     /locus_tag="PLO58_02605"
     CDS             complement(11980..12687)
                     /locus_tag="PLO58_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQO73711.1"
                     /translation="MDSQTIQNNLLIIKERIVQAAQKTGRRAEDIQIVAVTKTFPATL
                     VRQAYTAGLRNFGENRVQEAIPKIIALQDCPEISWHMIGHLQSNKVRKAIENFAMIQS
                     VDSLDLCNKIANISRALGRKVDILLEVNVSGEATKSGLPPEKVFSVVEQVAEMPELIL
                     HGLMTIGPLTNDRDWTRRAFANLKGIFDKIAESRINTGQFKLISMGMTDDYEIAIEEG
                     SNMIRLGRAIFGERRTS"
     gene            complement(12712..>13005)
                     /locus_tag="PLO58_02610"
     CDS             complement(12712..>13005)
                     /locus_tag="PLO58_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HQO73712.1"
                     /translation="NGRMVALREVVDTDDLMIITAKGVLIRLPVATLRTISRNTQGVR
                     LIKLDEGDTISAVTRVVESEDKNNGNGELEESDEITLLENDASDEVAETDEPE"
     assembly_gap    13006..13105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13106..15003)
                     /gene="gyrA"
                     /locus_tag="PLO58_02615"
     CDS             complement(<13106..15003)
                     /gene="gyrA"
                     /locus_tag="PLO58_02615"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQO73713.1"
                     /translation="MASQVNIEFQRDKIVPVNIDEEMRSSYLDYSMSVIVARALPDVR
                     DGLKPVHRRILYGMSELGLTYNRPTKKCARIVGEVLGKYHPHGDMAVYDALVRMAQPF
                     SMRYPLVDGQGNFGSLDGDSAAAMRYTEARMSRIASELLRDIDKETVKFVPNFDESLE
                     EPSVLPTAVPVILVNGSSGIAVGMATNIPPHNLTEVCNAAIYVIDHPEATVDELMQFI
                     PGPDFPTGAFILGKSEIKRAYETGKGVIKMRAQAFVENSKTGKESIIITEIPYQVNKS
                     NLIEKIADLVREKVIDGITDIRDESDKEGIRIVIELRRDAYAEVILNQLYNHTQLQES
                     FGINMLALVNGIPRQLSLREILQHFIDFRHEIIIKRTQFDLRQAEDKAHILEGLKIAL
                     DNIDEVIAIIKASKSPEDAKQNLMIRFRFSERQAQAILDMRLQRLTGLEVNKVIEEYK
                     ETLKLIERLKFILSNVSEQMLIIKNELAEVKEKYGDARRTQIIADEGEFSVEDMIAEE
                     DMVITITHNGFIKRFPVDASRRQNRGGRGSLGAQAKDDDFVRNLFIANTHDYLLVFTN
                     KGRCYWLKVHEIPRAGKGSRGRAIVNLINTQPGEEVRAVLGVKEFDDQHFVLMVTRNG
                     IVKKTVLS"
     gene            complement(15007..16938)
                     /gene="gyrB"
                     /locus_tag="PLO58_02620"
     CDS             complement(15007..16938)
                     /gene="gyrB"
                     /locus_tag="PLO58_02620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQO73714.1"
                     /translation="MSEIKFTDAAIDYGAEKITVLKGLDAVRKRPAMYIGDTGKRGLH
                     HLVFEVIDNSIDEALAGFCNKIVVTVHEDNSVSVLDNGRGIPIDIHKEEKKSALEVVM
                     TILHAGGKFDKGSYKVSGGLHGVGVSVVNALSEWMRVEVYRDGKIYTQSYSRGVPTSK
                     VEPVGKTNKHGTLVRFKADVEVFKKIEYEWEVLEHRCRELAFLNRDLEIVLVDERDKN
                     ENRFHYKGGLAEFVNFLDETRPAIIDKPITAEKEEDGVFVEFAMQYNTSYNENIFTFV
                     NNINTIEGGTHLVGFKTALTRTLNNYAQKNNLFKKESFQLSGDDVREGLTAVISVKVP
                     EPQFEGQTKTKLGNSEVKGIVDSIVSESLAEYLERNPTQGRRIIEKSLNAARSREAAR
                     KARELTRRKSALDIGNLPGKLADCSTRDPSMCELFLVEGDSAGGSAKQGRDRRYQAIL
                     PLRGKIINVEKTRIDRVLSNEEIRTIITALGTGFGDDEFDIQKLRYNKVIIMTDADVD
                     GSHIRTLLLTFFFRYLPKLIEDGHIYIAQPPLYKVVCKKQEYYAYDDEELEVLTKRFS
                     KDSTAKIDIQRYKGLGEMNPEQLWNTTMNPETRTLLKITIEEAAAADRTFSILMGGDV
                     EPRRQFIQKNAKYVKNLDI"
     gene            complement(16935..17210)
                     /locus_tag="PLO58_02625"
     CDS             complement(16935..17210)
                     /locus_tag="PLO58_02625"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HQO73715.1"
                     /translation="MESLGTAIARMLKIYGIEKPVRQNEVFFIWQDIVGEVIAKHAIP
                     EKVAYNKLFVKVDSPVWRNELNFRKEEILKRINSQIKNAQIKEIILR"
     gene            complement(17213..18328)
                     /gene="recF"
                     /locus_tag="PLO58_02630"
     CDS             complement(17213..18328)
                     /gene="recF"
                     /locus_tag="PLO58_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HQO73716.1"
                     /translation="MVLKNLRLVNFRKYTDSVFVFHEKTNFINGDNGSGKTSLLEAIH
                     YLSLTKSFRTNNDSEAVNYQSDYFNIFGEFLSDNNQTILSNMNYSKTEGRSFLMNKNR
                     VKRYTDVVGCAPVVILSPGLQKITEGGPAERRNFINRIISQTDRNYLVTLIEYRRRIS
                     QRNVVLNQYREKKRCRYDAYFEAQDEILARLGQKLFIGRQAFIADMDSIFKTVFARIT
                     HIGGTVGIDLKYNIKADPDNFISLFTKNLRERFSRDLLIGRTTCGPHLDEIIINYNGR
                     DIRQIGSQGEHKLILIALKLAEGAYIESHQNEKIIFLLDDLFAFLDGKHCRKIVEEIG
                     DKNQALITSTDVKMLRKYGFLDNPEKYKIFNLTTGEA"
     gene            complement(18364..19479)
                     /gene="dnaN"
                     /locus_tag="PLO58_02635"
     CDS             complement(18364..19479)
                     /gene="dnaN"
                     /locus_tag="PLO58_02635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQO73717.1"
                     /translation="MQFSVSQNLLLNNLNKVAKVTPARTTMPILNSILFILKDSNLTL
                     RASDIEITLSTTFTVNGQEDGSVAVPSRLIQEIVGEIREGELTFNATDEGEIKITAGK
                     GKYKIMGRPGEEFPALPSIINQNSILVNSKILSRIIQKTVFAVSRDELKPSLTGVLFE
                     VKADELRSVATDGHRLVYINRKDFSSPDYERQIIVPVKFLNLINTYLTDDNKVSLTIS
                     DNHIMMESDSTLIISRIIDERFPDYESVIPKDNDKELKADSDYLSAVLKRVSIFSNKS
                     THQISLSLNRELSKISTIDLETMSSADEILDVEYSGDDLTIGFNAEYLREIVRNIDTQ
                     KVIMKLKTPVSASLVIPENQEENEDLIMLLMPIRLSD"
     gene            complement(19800..21059)
                     /locus_tag="PLO58_02640"
     CDS             complement(19800..21059)
                     /locus_tag="PLO58_02640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQO73718.1"
                     /translation="MKIKLFFGIIFFATTLFAQTLIDGIAAIVGENIILISEVDQVAR
                     ITASQMNIDPNRDVANFNKIKRHVLNSLIDENVILEQARIETVEVKERDVENAINQRL
                     DMLVEQVGSVENLELLMGSPISKIKKDYRPIFKNQLIVEKMRSEKFSKVTVTRPEVEA
                     FYQTYKDSIPPIPPSLDFSAILFKVTPGPEEEMAAKKLIDSLYTLLKNGYDFEYLAKN
                     YSEDDASAPYGGDLGYIKRGAFIKSFEEVAFSLSPGEISDVIKTDFGYHIIQMVDRKG
                     ESIRVRHILIKIKVSDKNYEDRYRFADSIRTMIINNEISFDSAVVKYSDEPNAKSTLG
                     RIRRIPLNQIQNTDFINVLSNMKVGDISPVFESSPGYYILKLNNVYDDSWPTIERFAL
                     EFKKNQLYQEWLNKLKEDIFIDIKVNY"
     gene            complement(21056..21880)
                     /locus_tag="PLO58_02645"
     CDS             complement(21056..21880)
                     /locus_tag="PLO58_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQO73719.1"
                     /translation="MRKDFLLMIFIFLWAACGNHKEDYIARVGNAYLTNEDLTRMLPD
                     GGLSKDIDKNYLNSVVSNWINKEILYQKARQYHFDREEGVQARVADYYRDLTIDSYVR
                     FFLQTNVTISEDEIRDYYLKNKKSFIRDREEAKIVHVLVQDFNDALAIKSAITSRNKN
                     ALDAFYSKYKFETSVVKRGESLEEIDRNIFETSPRSIIGPIASNYGFHIVEVLARYPI
                     GSTRTIDEVRDEIYQILTQKKIQDQYNALVDSLVRNANYEIKDENISNFLNSIGSR"
     gene            complement(22027..25260)
                     /gene="mfd"
                     /locus_tag="PLO58_02650"
     CDS             complement(22027..25260)
                     /gene="mfd"
                     /locus_tag="PLO58_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQO73720.1"
                     /translation="MKHLDDRIENLVSTFSESSAFLNAVKAFYRSNAGFTVAGLSGFA
                     TALFLYSFYKKIDQPILLITNDSNEAEYIRDDLELFNLDDSVCYLPEPPNSSFLFSDT
                     ESLNAYFTNDTLQKLSTGQNTIIVATLSSLKSKFPLSDDFNKLKRTYHVNEIVNRDAF
                     IEYLVHLGYTIAPAVESPFEYCIKGGIIDFYPPESLHPYRIEFFGDKIESIRTFNPDT
                     QLSICNLSSFTVQAPAHSSLDNQNSTNIFSYLKKDTVVFCLHAEKLMDTDEKNTTDIF
                     TYLSSYRVINHYDLLASDVDFRVNPQPQTNSIKAFREHLLTVLSAQPDARISLFCANS
                     MQVERLKELLGLEKLNYFDFSISAGAELPEAKLYFYTDHQIFKRERRINFFKKYSPDF
                     PVEQFSPHEISPGDLMVHINYGVGRFAGLDKIAAFGSIRECLCLEYSGGDKVFVPLEK
                     LNYVQKYTGNLQNPPPLNKLGTTEWERTKLKTQRAIDEISKDLVELYAKRISAPGYAF
                     PPDNELQYLMESEFLYEETPDQLTAIQAVKEDMEKPRPMDRLLCGDVGFGKTEVAIRA
                     SFKAVSASKQVAVLVPTTILADQHFAVFSQRLQKYPIRIALLSRFVSRKMQSQIIRDL
                     AAGKIDIVIGTHRLLSKDVSFKDLGLLVIDEEHRFGVKDKERIKFLRESVDILALSAT
                     PIPRSLNFSLIGVRDFSVINTPPISRLPVITEVITFDMNIIKKAILREINRGGQVFFV
                     HNDIKSIAALSHKLALALPEISVDYVHGQMNERELEKTMSAFINNEINLLVTTTIIES
                     GIDIPNANTIFINQAHHFGLAQLYQLRGRVGRSSRRAYAYLIVPEPSRIKPDALRKLQ
                     TIKKHTALGSGYSVALQDLEIRGAGNLLGTSQSGNNIAAIGYELYMKFLREALDEIKS
                     TQPAYPEKLRLETEVFCPLPAYFPDSYISDSSARLEYYRQLTLADELSTVDLISGQIR
                     DIYGKLPSEATTLFNISKIRLLASSLGIKKVNIAEKSTSFIWDESFIPPAETDLIQAI
                     QKAASDLGLSYKFNPYNTLYLTVYLKSNFDFDKITRFLNLLRNTLNL"
     gene            complement(25257..27137)
                     /gene="mnmG"
                     /locus_tag="PLO58_02655"
     CDS             complement(25257..27137)
                     /gene="mnmG"
                     /locus_tag="PLO58_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQO73721.1"
                     /translation="MKHLDFDITVVGGGHAGIEAALAAARLGLSTLLVTQNISTIGRM
                     SCNPSIGGLAKSHLVAEIDALGGAMAKLIDRTGIQFKMLNKSKGRAVWSPRAQADKIH
                     YSLLARNTLSNQDRLTLLEDTANSLIVDKQTIKGIHLIHNGDIFCQAVILTCGTFLNG
                     LIHVGLQQFHGGRIDEEPVSGLTEDLARYNILSGRLKTGTPPRVLAESIDFSRTMPQY
                     GDQDPIPFSFSTQNFSPPNIPCFLTRTNTVTHNFILNNLDRSPLYSGIIKGIGPRYCP
                     SIEDKCVRFKERTSHQIFLEPEWKNSNEYYVNGFSTSLPIDVQMKALRTVPGLEKAEF
                     IKPGYAVEYDFFPSYQIKDTLETKSLNGLYIAGQINGTSGYEEAAALGLMAGINAALS
                     IQGREPFVLQRHEAYIGVLIDDLITKSPNEPYRMFTSSAEHRLMLRFDNADRRLARKG
                     YEVGLLPRETYESTQHKIEVIEEIISFLSSTNISPEAAKVILPNNDAHSIGCNLSLYK
                     LLCRPDVALASLVPFLSSEIKNKIAHFPKIIEEIECDVKYAGYIKHSQALIESMSKNE
                     NIKIPPDFDYSAVRSLTNEAREKLIRFRPQTLGQASRIPGVSPADVAALFFLLHKHSG
                     LT"
     gene            complement(27147..28499)
                     /gene="mnmE"
                     /locus_tag="PLO58_02660"
     CDS             complement(27147..28499)
                     /gene="mnmE"
                     /locus_tag="PLO58_02660"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQO73722.1"
                     /translation="MSTENTIVALSTPPGRGALAVVRLSGPKSPDIVHKLTKTTTQLK
                     PRVAQHLNLYKNGELLDDAVIIYYKAPMSYTGEDLVEISCHGSPFIVNQLIELCLRYG
                     ARLAQPGEFTRRAFLNGKFDLTQAEAVADLVFSQTQASHQAAIKSLEGTIGKEFAELR
                     SKIIELITILELELDFSDHEIDTIPPQELSKSIDNILKINSKLLKSYSCGKILKQGAL
                     VPIIGPPNSGKSSLLNAFLNEERAIVTPYPGTTRDSLEEAIQAGGYMIRLLDTAGIRK
                     TKNPIEKIGIDRTKKNIKTGDIILFVIDATQPIKREWLEYYNSKNTIVVINKIDIAPQ
                     KSLTKYEKHFSQYHHIFISAKKRQGIHKLTEMIVDSISQQIGTADSLIITNQRHATGL
                     RTAKKYLLSAQKHIRQSLAPEMIIADLRIALNEYDKILGKTSNDEILNNIFQHFCIGK
                     "
     gene            complement(28519..30279)
                     /gene="yidC"
                     /locus_tag="PLO58_02665"
     CDS             complement(28519..30279)
                     /gene="yidC"
                     /locus_tag="PLO58_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQO73723.1"
                     /translation="MDRKSLIGFVLIFVIILTMPYYFKIISKNSPEVTQEKVIEKTPE
                     GVISETIPAQLSESHPSSEVQRPASLGSEKPDTLTIENDLYRLKISSRGGGTITSVVL
                     KNYNKIIGRDTSLVEMIPPFEARRLFIKYIKSNGDSVTIDQNFRIFTEHQETSKNYYK
                     ISDGDSIIIQFQLPEGDNIIVSRTFVFYGDKYIINSYNDLGGILDEMASDKYDLVWEG
                     GIAYTEAMIKEDVSYSRASACGGGETENLDVKPGKTGSASLVGATYWTAIKTKYFVAA
                     LIPHQPAEGYRLTGLGVPLQGKDFQKIFNMYLTLNASSENAVKIYLGPLDYSIIKGLA
                     PNFEHLMNFGFSLIRPISKGILWLFTKMHLFIPNYGWVLIIFSVLLKIVLTPLTNSST
                     RSMKEMQNIQPQLNALQEKYASDPQRLNNERLKLMREHGVNPMGGCLPLLLQMPILWA
                     LFIVFRTTIELRQAPFILWITDLSAPDTIFTLPFSIPIYGSQVNVLPFIMAISQLVQQ
                     KISGASGGKQQKVMMYFMTIFFFFLFNQFPSGLNLYYTLYNILSIIQQKYFPPKPKPK
                     KPHKTNSQRLRQYQTRMRGR"
     gene            complement(30282..30512)
                     /gene="yidD"
                     /locus_tag="PLO58_02670"
     CDS             complement(30282..30512)
                     /gene="yidD"
                     /locus_tag="PLO58_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HQO73724.1"
                     /translation="MKIFNTLIILIIKTYRLLLSPLFPNTCRFTPTCSQYALEAFKKF
                     NFFKASWLSLTRILKCHPYHTGGYDPLPTPKE"
     gene            complement(30518..30892)
                     /locus_tag="PLO58_02675"
     CDS             complement(30518..30892)
                     /locus_tag="PLO58_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQO73725.1"
                     /translation="MAEKSSPEDELKDVRSYRPNLPRRERLAKWDQRAQIIHNGKPLK
                     YDCLYISILPQDDWKLSIVIKKECGNAVQRNLYKRKIREAFRLCKPYCRRPAAIAITV
                     FKKPDNLQVNTLSEILINNLNL"
     gene            complement(30834..30968)
                     /gene="rpmH"
                     /locus_tag="PLO58_02680"
     CDS             complement(30834..30968)
                     /gene="rpmH"
                     /locus_tag="PLO58_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQO73726.1"
                     /translation="MKRTYQPSNRKRKNDHGFRKRMSTNSGRKVIARRRAKGRKVLSA
                     "
BASE COUNT         8617 a         6637 c         6183 g         9408 t
ORIGIN      
        1 aattactctt ttttgaaact ttccttgacc ttctcaatgg cttcttcagt gctcttttga
       61 agtttatcgg taaccttttc agagtcattt tctgttacgg actctatatc tttttgaatc
      121 cggtcaatga tttttttcgg ctgagcgatt ttgtatgtca agccaactgc tagcgccaga
      181 atgataaatg agatgactgc ccacttgagc aggcgcgcta taaccattat gataaataac
      241 accaatgcgg cagtcagcgc gactccgata atcggatgag aagttaggta gctaatccac
      301 acatccataa taatccctcc cactttaggt taaaagtaat cccattaatt cgcgaatcca
      361 cttgcgctct gcctgcaaaa tgaccagcgg tcgggttaat agggcgcgct gaacctggtc
      421 attggtattt gagagagcgg tcagttcatg aactttgttc tgaatctgcc gacctagctc
      481 ggtatcatag agcgttaacg cctctcggag cgcctcctta ttaaggacac cactataagt
      541 tagaccggtt tcaaaaagtg acggctgatg ttgtggttgg gaaagggctt tgaagatttc
      601 ctcattaaaa atctgacgcc ccttgggtgt aattgaataa attctgcggc tgggtagtcc
      661 tttttgtggt tccaaattac tcatcagaag accaattttc tgcattttgt ttaaaacggc
      721 gtaaatcgtc gagaatccaa cgttggtgcg ttcgcgtatc ccgcgttcgg cgataatctt
      781 ctcaatatta taagcgtgag ttgcgccctc tgagacaatc ccaagtatgt tgagctccag
      841 attagtcatc attgttctcc tttaaaaatg agcggataca accaagatta gcatatatag
      901 ttcccgactt ttactgctcc ggataaagta caacctctac gtagttatca agcggcaggt
      961 agcgtcgggc ggcattctgg accatttcct tcgtcagatc ttctactaat tgaggataat
     1021 gcaggatcgc cattggattt tcattgttga aataatagaa atgaaggtta tccagccaaa
     1081 agcggttctc tttcagatta gtctcgtagg agcgtaaatc aatttgccta attttggcca
     1141 gatcttcatc gccaagtggt tcgctcttta ggttttcaat ttcttggaag atgacggagg
     1201 ttaattctgt cactcgctcc gggtcgcaac caaattgaat agtcagacga aattcaggat
     1261 atgggtattg cttggaagag gaaccaacat aaataccgta tgtgccgccc ttttcttctc
     1321 tgatttgttc tcgcaattta attcgcagaa catcggttac cgttgctaat tcaaaacgat
     1381 tctgacgact ccaatcaaaa ttaccgctga aggtaatctg gacgtaggat tgcttctcga
     1441 gccctttata aaccgccttc tgcaccacgc cgttcggtgg acggatacct acgtcgcgcc
     1501 aattctcctc acgattattt gtaggtaaat tagccagata gcgcatagcc atctgtttcg
     1561 ccgaatctaa ttccacattg ccaattagga aaaaagtaaa atccccagct tctccaaaac
     1621 gatctcggaa aatgttgtat gatttattca aatctatctc atccagcatg gcaatcgtcc
     1681 atgggcgcgc ccggaaatga tactgagata gcgtcaccct gattgtatcg ctgaaagccg
     1741 caaacggatc ggcggcgcga ttgacaatcg agtttttcaa tttttcgaga taagacaggt
     1801 aggcagtgct gtctttgcgt ggcgcagtaa aataaagata tataagttgg aacatagttt
     1861 ccaggtcttc aggcgaggct gaggcactga aattttccgt caattccgcc agagacggtt
     1921 gcaccgaaac cactttaccc gccaattttt tagataacat gacgctattg aattctccca
     1981 agccgctttc agtaacgatg ggaacactgc taattgcggc aacataattt gaatcgggaa
     2041 ccagtgaggt tccgccagga ctataagccg aaagcagaat ctcgtcattt ttaaaatcag
     2101 tcggcttaat gataactttt accccattac tcaatattag ttcagtaaga ttcagggaat
     2161 caatgtgagt ctcctgcagg atgcgaccgg gcggcggcaa ttgcctgacc agatcgcttt
     2221 cagccacctc atcaatatag gctgtcactt cctgccgctc caccttttcg aaaatttcgc
     2281 gtaattcggt ttcggtcggt attttcaaat ccggtttctc gggagcagat actaaaacta
     2341 cccggttaat atcagtaacc cattctttag ccagaacatt gacctcgggc agtgctatct
     2401 ccggaacgta ttttttgtaa aattcatatt cgagttcaat ccccggtact ggttcattat
     2461 caagaaaatt acgtatatat tcactggcaa aactgcgtga gtaaatctta tcccgctcgc
     2521 ggtaaatctg ttccatatta cgtaaagctg tgcttttagc ccgttttagt tcggtttctg
     2581 taaatccaaa ttcccggacg cgtttcaatt ccgtaagaat agccgtaaat ccctcttcaa
     2641 tgccattatc ttttacaacg gctccgatgg aataacaatc cttggagcgg actaatcgcc
     2701 ccttttgaga cgccgctcct agaaaaggtg gttcggcagt tttggttaat tcctccagac
     2761 gttcattcag catactgttg tataaatttt caacaatcga ttggcgatag tcacgtatat
     2821 atttttgttc tgttcgcggc aatttaataa gcaaatccac ttgattgcgg gttagctcgg
     2881 ggtcggtctg aattgaaaac aaagtttcgg tatgatcagg cacgggcgga attggacgag
     2941 gcggtgcatc agctggtgat tttaagccaa taaaattttg ctcgatgaga ttcttgaccc
     3001 tgtcctcatc aaaatcaccg acggcaatga ctgccatcaa gtcagggcga taccagtcgc
     3061 gataaaattt tcgcaatgta tcgtaatgaa acgtatcaat tacggctatt tgcccaattg
     3121 gatggcgttc ggcatataat gagccccaaa aaattacctg aaattctttc tcctggatac
     3181 gcatttcggc tccgcgccca agccgccatt cttcgcgaat gacaccccgc tctttatcaa
     3241 tttcttcatc ttccagacta atctgagaag cccaatcagc caaaatttga aaaccctttt
     3301 ccaataaaga atcacggtca gtgggcacgg tcagcatata taccgtttca tcgaaactgg
     3361 tataagcatt tagatgcgaa ccgaatggca taccggctaa ttccataaaa ttgataattt
     3421 cctgcttctt aaaacgggct gtaccgttaa aagccatatg ctccagaaaa tgagctaatc
     3481 cccgctgatc ttcctcttcc agaactgaac cggcgttaac cactaagcgc agttctgcgc
     3541 gcctttccgg cttttcattt ttgcgaatgt aataagtcaa gccgttatca aatttccctt
     3601 tggtaatttt cggatcaagg gcaatttcag aatctaattc aagagattgg gataggctcg
     3661 gcggagtagg cggaatcttt ctggtcaact cgcttgtgct acaggctatc atcgtagtaa
     3721 aaagagccgc cacgacgatt gattttggta tatgaacttt cacgttgcct ccttttataa
     3781 aaattcgatt tgcttaatta tttatctcaa atctccgata acgctaatta tgtaatcctt
     3841 caagatactt ggaagcggac agagctccaa cagcgccgtc gccaacggca ttggcaattt
     3901 gacgaaatgg cttggagcgc acgtcgccaa ctgctataat tcccggaacg tttgtgtgca
     3961 tttctgcatc ggttattata tgtccgtcat catcgagatt caccaagcca cgcacgaact
     4021 cggtattcgg cacccagccg ataaaaatga atacaccttc agccggaagg ctgatttttt
     4081 caccactctt gacattgcgg atttcaacgc cttcgacttg ctcagctcca aaaatattgg
     4141 aaaccaccga atcccagata aatctgatct tttcattctt aaaagctcgc tcctgcaata
     4201 ccggttgcgc ccgcaatttg tcgcgccggt gaataaccgt cacccttcga gcaaattttg
     4261 tcaaatatag cgcctcctga atagcggcat ttccgccccc cactacaatt aattccgcat
     4321 tgcgatataa tggcccgtca caggtggcac aataggacac tccccgtccc aaaaactctt
     4381 gctcggaggc gatgccgaga ttgcggggaa cccctccggt agcaatgatt accgctttag
     4441 caagaaaacg acgattatcc gaaattactt caaagcattg ccttcctggt ataattttgc
     4501 tcacgtcttc aaactcgctg atattggttc caaaacgatc cgcctgagcc cgtatatttt
     4561 gcattaattc cataccactt attccatccg gaaagcctgg ataattttca atccgatcaa
     4621 ctatagccgg gatgccgccc gtcatagatt tttccaataa gatggtgctg agcatttcgc
     4681 gcgcggtgta gatagccgcc gtcagtccgg acggtcctcc gccaatgatc acaacatcgt
     4741 aaaccacatc ttttgccaca aattattcct ttcttttact attttgaaaa ttagttaaaa
     4801 ttatccatgc agagttacaa tttttaataa gccggtttaa attttgatag catttggttt
     4861 aggcttaata tatttataaa ttttatcaaa tgcatagcga acatgaaata attttgattg
     4921 gcgatcgagt tttaatcgat cctgatgaag atcagaacaa aacgccaagc ggtttattct
     4981 tacctcccgg agtgaaagaa aaagagaagg tgcagggcgg cttcatcgtt aaaactggtc
     5041 cgggtttccc aatcccccat accgacgata ttgaagatga accctgggaa gaaggggaag
     5101 atgagccgca ctatttgcct cttcaggctc aagaaggtga ttacgccatt ttcttgcgta
     5161 agtccgctat cgaaatcgaa ctcgaaagca aaagatatct gatagtttcc cattctaata
     5221 tcctcctatt gatccgcgaa aactacctcg aataatactg cttccaaatt tcatggttta
     5281 acaacttggg catggtattg ctaaaataaa aatgcggtcg gaatggccgc attgtgatct
     5341 atatgcaacg aacggagagc tttaatctaa ccagttgatg tcaacacttc caagtccaac
     5401 ctcaatttcc agatagattc ttttgttggc ggtttcccag ttaccactgc ggtataggtt
     5461 ttcgctgatg cgatcaaaac catgcaaatt aagcgatgac aggaaggaac tttgcgaacg
     5521 gacttcaacc gccacattat ccgggacttc gataatgacc gaaccgagtc cgacttcaat
     5581 cttacctcga atatcctgac tcaggctacc gtcaaacacc aattcggtag tgccgagacc
     5641 acattccacg ttaaagcgtt ccatattggc gtaactaaga cccttaacat taacgctacc
     5701 cagtccagtc tcaataacca tattccgcat attatcctga ttcttatctc taaatatgac
     5761 attaacgtca ctcaacccac aatgcaattt caaatcccgc acccgtaaat cggtaaaatc
     5821 cagccgaccc tctcccaagc cgagatccac tgcaatgtaa atcggtattt tttgagaaaa
     5881 ttctaaatgc catttattta attctttatt tacttgggaa gatttgagat ttcttttatc
     5941 attcttattg gattcgtcgt acttttcggt ggacattttt aggcgaccac gattgcccag
     6001 tatcttataa tccacaatcg gcttataaat acgttgtgaa taagtcatta ttgaatgcac
     6061 caggtattta ggatcatcgt gacttttgag atcaagagtc cccaaaccaa agctgatatt
     6121 aacgtccagc tcttcttcac ctttatacgg aatatcataa tgttccgtga agtaatcttc
     6181 cgctgataaa accgctatca tgaatatcaa gaggataatt aattttcttt tcatattatt
     6241 ttcatcacct cgattctcga tcggccttta atatgtttat gttgccgtta atcgtcctaa
     6301 gcctgactag actgccacct tggttaattt tgcctctggc atagatgtat atacgcgagc
     6361 ttttaatttc atgactcttc tgaaccggaa aatcggagtg gatattgaca tcggcataac
     6421 gacgactggt aacggtaatt tcagcatcaa tatcggcatt aagattagcc ggtaggataa
     6481 cttctatctc gccgccgctg gtttccaaat caatggcatt attcttcacg tcagaattaa
     6541 ataatttacg agcttctata tcacctccgc tggtacgcgc atagatgtat ccgaccactc
     6601 ctttggctag gatatcaccg cctgaagtaa tggcttccag atcatatgcc cggtcaacct
     6661 caatattgcc accagaagta cgagcataac aataaccttt tatgagtcca atatcgatgt
     6721 tgccgccgga ggtagtagcc ttcaaattct tatttacctg acgcacttct atatcacctc
     6781 cagaggtgtg aatttctaaa ttgccttcaa tctggccaat gattatatca ccgccggagg
     6841 tacgcagggt aagttcggcc tcaactttat cggcgccgat atcgccaccg ctggtcttag
     6901 ctgaaaaggt tttcgcccgt agattgacaa tatttatatc tccaccagaa gtgatgacct
     6961 ggccattacc ctgtatttta tcaaggttta tatcgccacc ggaagtgtta gcccagagtt
     7021 cgccatcaaa taattcgatc tctatatcac caccagaagt gttgacggat aaattacctg
     7081 caacttgttg aattttaata tcaccacccg aggtatggct tttcaattca ccactgcatt
     7141 cgctaatctc aatgtcgccg ccactggtat ttagattctg aagtccacta accttattaa
     7201 tattaatgtc gccacccgaa gtctgaactg tcgtattaaa agtcaatggc actttcacta
     7261 taaaatcgct ggagtagcgg cgcgaatgtt ccttgccagt gactattact ttattgcctc
     7321 gttgttgata gcgtgctttt tcctcattta aaatttggcg cgcattaacc tcagaatagg
     7381 cgtctatttt aaacttctcg acgatcatca cgtcccggcg agcctcaccc tcgatcgtga
     7441 catccccgct gacacgctcc ataacaagat cgcccttact accgactgca aaagttttaa
     7501 cgatgtttcc gacatattgc ttgccatcaa attcaaattt gtcggggctg gcgccgactg
     7561 agccaatcat caatagaatt aggaataatt ttttcggttt catcgaatat gctctctatc
     7621 ttttttcccg atcgttttta tcataaaatt tgtctaaagc caaaagagcc atctgtctta
     7681 tttcttcaga ttcatcacgc gccgccacca aagctaatac tggcacaacc tcctgctctg
     7741 ccttattaac caaattttcc agcgctattt tacgaagaga tgcgcttgaa tctcgcaggg
     7801 caactaattg gaatgcttcc tgaatttgct tggtcatctg tcgtttctcc aacaattggg
     7861 cggcaaataa gcgtaccgat tcattattat catggagcat agccgccaca aaagttgcgt
     7921 caacgatagg gttgggttcg atctggttca ataggcgcag tgccaaaagg cgtttctcgg
     7981 catcttgatt atgacgggcg gcatacatta acatgttttg aatggtggga tcatctaatc
     8041 cacctctaat ggcaaaatct tcctttactc tgacattaac ttctactaat ccattctggc
     8101 tggttcgata ttcactttga agtaagtcag catcttctat cccccctgat tttagtaaat
     8161 agttagtaaa ggctttttga atattttctt tctccggatc ggtgaattta tagttgtaat
     8221 taacaaagtt ggcaagtatt tccggttgtt tgggtaagtt ggctctaaac cctactcttc
     8281 caataaaaat gccgattacc agaattaaac tggcggcaat tagttgataa cgcggacggg
     8341 acaagccgat tgtaatcgtc tcaaacattt cctcaaaata tgttttgaca tttaccaatt
     8401 tcggtcggcg cgattcggat gaaatttttg atattacttg cttgttcaat gcttccaatc
     8461 gtcgggcgct gggcaccggt tcagtagcgt agtctatttt cttggcaata atttgtagac
     8521 gttctatttc ggcctggcag acttggcact cgtccagatg atgtttaaaa tcagttcgtt
     8581 cggattccga tagttcattg taaaagtata ggattgaaag ttctttaaaa tgtttatcgt
     8641 gcttcatatc taaatccttt cttatgattc agttaaaagc gtttgtaatt tgtgagttgc
     8701 gcggaataaa tagcgcttga cggttccctc ggtgcaatcg agcaaaatcg caatgtcctt
     8761 tatttttttc ccttcaaaat gcttaagaat gaatactact ctttgttttg cagatagttg
     8821 ctggagagcg gcgtctattt ggtcctgaat ggaattgctg aatgttaccc gatcggcttc
     8881 gtcttgagcg tcggcaggaa tggtctccca caggtagtcg tcttcaccgg gatcagctaa
     8941 agtcatacgt ttgcgtttat tatatagatt aaaagcttga ttagctagta tgcgataaat
     9001 ccaggtaaaa aattcactct cgaagcgaaa ttttcccaga ttctgataga ctttaataaa
     9061 ggtctcttga taaaggtctt ccgcatcctg atagttgcga gtaaagcgga aggctacact
     9121 catcacccgc tcgccatatt gttccattaa acgtgcgaat gccattttat cgccactttg
     9181 aatattctgg attaattcga cttcttcgtt ttccatacat ctacccgctt tagattcaca
     9241 tcggcgataa aggttgtcaa acttctgttt agtggacaat aatgaattta tttattttga
     9301 agtattcaga gcttcgtagg catacaaagt aaattccaga gccaacgcgc gcgccggatt
     9361 gattatgtaa attccaattg atgacttctt tttggccgcc aaatttgcta accagccttt
     9421 tttcaaaaac gatttgtccg ttgagattta tgacttgtag gtaagccgga gcggtagcgg
     9481 gcgtggcttt atgccagata aaataaaaat ttatgttgct attagccggg ttggggtaaa
     9541 cccaaatctc atcgaaagat agttcgcctt tcgggaaact cttgggttgg taattgacga
     9601 tactttgata gacatctgga atgccccagc cgtatctatt atctgggttt tgggtgcgat
     9661 cggcataacg tttcagttcc gtgataattt ctgcgggggt cagctctggc tgactctcca
     9721 acaatagcgc cgcggcacca gcaatcaacg gtgtcgccaa ggaagttccg ctccctgtac
     9781 gataagctcc ttgcgaatta ctggcgtaat aagtcgcaac gcccatagca cataaatccg
     9841 gtttgattcg gccgtcgtag gttggaccgt gagaactgaa ggaggctatt tcaccctgcg
     9901 catcaaccgc accgaccgtc aatgctccaa aagcatcgcc gggtgtgagc agtccgccat
     9961 cctcaaaatt attagcgtcg ttaccagcac aacagacgca gagaatgccc cgctcgaatg
    10021 cccagttaac agccttagtg gtaatggcag ttcgcccgtc aagatattca aaaggatact
    10081 cagaaccatc agcaaaatca cgatacccga gagataccga tacgatgtca gccccccaat
    10141 aatcacacca ctccaccgcg gcgacaaaat catcttcctc acgatgaatt tcggtatcaa
    10201 ttctttcggt tttggctagt aaaaattcag cgccataagc cgctccaaga taatcaggtg
    10261 gattatatcc gccgactaag ctccagacgg cggttccatg ttcactctgc cggctgtttc
    10321 gggcggtatc cgcggcttct tcatcgctga cgttgtcatc atcattgata aaatcatatt
    10381 cggctatcaa gcggccggac gataaaattt cactaaaaac ctcatggtct ttaaaaaatc
    10441 cggtatctat aaaaccgatt cgtacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnncaata atagcccgta attcttcacc gggaacaagg tcagaagcgg tcttcttggc
    10621 taacgttccc attttggcaa ggcgtcgttg agttttttcc ggatattcga gttgcgtaaa
    10681 atattcatta taagctggtt cgttcgataa ataaatccag gctcgtatag tcggttgatt
    10741 ggtgaattta tctattgtaa ccggatgtcc agcccataca atgttaggaa ggattaatag
    10801 tagaaaccca aacacccgaa aacgatgaac gtatatgttc cggatgcgct gaatcatttc
    10861 agcaggcaat ctattaaaat acgagaaaat aaaaaattgt tattcttgag gaaggcagtg
    10921 gagacgttaa aatcagctac caatccaggt gagcaagtaa caaacccaat tgtttaccat
    10981 caatatctga aaataaaatt agtccagcgc attgacgtct agcatgccta acggttatcg
    11041 ctccggcgaa aacatcctga gaaagtgcgg caatttcgtt ttgcactatc aattgttctt
    11101 ccagagatgt cgagctggtc attgaaacgg gtacaagcag aatacctttc tgtaaaacga
    11161 gaccggcttg tttaccagcg ataaggacat cctcggttaa tttcgggctg tacaaatcgg
    11221 tcatatctgg aaaacgcgtc ccgaattctt catcattagg accaaccaag ggattctgcc
    11281 cggaaacatt aatctgatct acaactacta ttcctgcgga cttttcggac cgccgcgttg
    11341 gcacccctcg cccaaccaat aaaaaacgtt cagcgccatc tttaatcgcc gagcgaacgg
    11401 cttcacgcca ggttttaaca tttgccccat tatcaagaag aatcatttta aagtttaact
    11461 caccctctgg tatttgcggg catacagctt tattcattat aagtgcgtta taaattttat
    11521 ttagggacat aatttttcag gttgtttcct cagatgactc gttaagggtg ttatcttctt
    11581 ccgaccaatc tggtgcttcc tcgagccagg taagtatttc cgcctgattt ttcaagacac
    11641 ttcgtaatcg ttgtatcaat ttattctgtt cgaggttgag ctgatcaatt gcctcctgta
    11701 tttgcctctt cttggtttct gcattggcaa ttacttcaga agcaatagac tcagcatcca
    11761 agataatact tgccgcccga tcttgagcct cttgcaaaat tttatcgcgt cctttgcgag
    11821 tctggtcaaa ctcatttact atggcttcca atttctctag cttttcttcc atctgagcaa
    11881 attcatccgc caacatttcg gtaaaatatt taacctcaac cggatcatag ccacggaata
    11941 ctttgcggaa tcggcggtcg cggatttcat cagtcttgat catgatgttc tgcgctcccc
    12001 aaaaatagcc cgcccgaggc gaatcatatt tgagccctcc tctatagcaa tttcataatc
    12061 gtcggtcatt cccatagaaa tgagtttgaa ttgaccggta ttgatccgac tctcagcaat
    12121 tttatcaaaa atgcctttaa gattggcaaa tgctcgccga gtccaatccc ggtcgttcgt
    12181 taatgggcca atcgtcatta atccgtgtaa aatgagttct ggcatctccg ccacttgttc
    12241 cacaacggaa aatacttttt cgggaggtaa acctgattta gtggcttcgc cggaaacatt
    12301 tacttcgagt aaaatgtcca ctttccgccc cagtgcacgg ctaatgttgg caattttatt
    12361 acacaaatca agcgagtcaa ccgattgaat catagcaaaa ttctcgatag cctttcggac
    12421 tttattgctt tgaaggtgtc caatcatatg ccagctgatt tccgggcaat cttgcaaggc
    12481 tattattttc gggatggctt cttgaacgcg attctcgccg aaattccgta aaccggcggt
    12541 ataagcctga cgaaccaatg ttgctggaaa tgttttggtt acggcgacaa tctggatgtc
    12601 ttcggctcgc cgccctgttt tttgagccgc ttgcactatg cgttctttga ttatcaaaag
    12661 gttattttga atggtctgcg agtccaagaa ggctaatcct tttagattag attactctgg
    12721 ttcatcagtt tcggcaacct cgtcagacgc gtcattttcg agcagggtaa tttcgtcgga
    12781 ttcttccaac tcgccattgc cgttgttttt atcttctgat tccaccacgc gagtcacggc
    12841 tgaaatagtg tcaccttcgt ccaattttat tagccgaacg ccctgagtat tgcggctaat
    12901 ggttctgaga gtagcaactg gcaggcgaat caatacgcct ttggcggtta taatcatcaa
    12961 atcgtcggta tcaaccacct cgcgcaaggc taccatccga ccgttnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnntcgga caagaccgtt tttttaacta taccgttgcg
    13141 ggttaccatt aggacaaaat gttgatcgtc gaattctttc actcccagca ccgcccgaac
    13201 ttcttcgccg ggctgagtat tgatcaaatt gacaatggcg cggccgcggc taccttttcc
    13261 ggctcgcggg atttcatgaa ctttcagcca gtaacagcgt cccttattgg taaaaactaa
    13321 aagataatca tgggtattag caatgaatag gttgcgcacg aaatcatcgt ccttagcctg
    13381 cgctcccaga ctgccgcgtc cgccgcgatt ctggcgccgc gaggcatcaa ccgggaagcg
    13441 cttaataaaa ccattatgag taatagtaat caccatatct tcttcagcaa tcatatcctc
    13501 gaccgaaaat tcgccttcgt ccgcaatgat ctgagtgcga cgcgcatccc catatttttc
    13561 tttgacttct gccagttcat tcttaataat caacatctgt tcactgacat tgctaagaat
    13621 gaactttagt ctttcgataa gtttaagagt ctccttgtat tcctcaatga ctttattgac
    13681 ttccaagccg gtgagccttt gtaaacgcat atccagaata gcctgagcct gtcgttccga
    13741 aaatcgaaag cggatcataa gattttgctt ggcatcttcg ggagactttg atgctttaat
    13801 aatagctatt acttcatcga tattatcgag agcaattttc aaaccctcga ggatatgtgc
    13861 cttgtcctcg gcttgcctaa ggtcgaattg tgtccgcttt atgataattt catgcctaaa
    13921 atcgataaag tgttgtagaa tttcacgcag agacaactgc cgcggaatgc cgttgaccag
    13981 cgccagcata tttattccga agctttcttg taattgagtg tggttataaa gctggttgag
    14041 gataacttcg gcataagcgt ctcggcgtaa ttcgatgacg atccggatgc cttccttatc
    14101 ggactcgtcc cgaatatcgg ttataccgtc tatgactttt tctctgacta gatcggcaat
    14161 cttttctatc aaattagact tattaacctg ataaggaatc tccgtaatga tgatgctttc
    14221 cttaccggtc ttgctattct caacgaaagc ttgggcgcgc attttaatta cgcctttgcc
    14281 ggtttcataa gcacgcttaa tttcactttt accgaggata aaagcacctg ttggaaaatc
    14341 cggacctggg ataaattgca tcagttcgtc aaccgttgct tccggatgat caataacata
    14401 tatagcggcg ttgcagactt ccgttaaatt atgcggcggt atattagtcg ccataccaac
    14461 cgcaataccg ctcgaaccat tgaccagaat tactgggacg gctgttggca gaacacttgg
    14521 ttcttcaaga gactcgtcaa aattgggtac aaatttgact gtttctttat caatgtcccg
    14581 caataattcg gacgcaatac gactcattcg agcttcggta taacgcatag ccgccgcgct
    14641 gtctccgtct aacgagccaa agttcccttg gccatctact aatggataac gcatcgagaa
    14701 aggttgtgcc attcggacca gcgcatcgta aactgccata tcgccatgcg ggtgatattt
    14761 accgagcact tctccgacta tacgagcgca ttttttggtc ggacggttat atgttagacc
    14821 caactccgac ataccatata aaattcggcg gtgtaccggt ttaagtccat ctcgtacgtc
    14881 cggcagtgcg cgtgctacga ttaccgacat ggaataatcg aggtacgaac tgcgcatttc
    14941 ctcatcaata ttgaccggta cgattttatc tctttgaaat tcgatattta cttgtgatgc
    15001 cattttttaa atatccaggt tcttaacata ttttgcattt ttttgaatga attgccgacg
    15061 tggttcgaca tcaccgccca ttagaatcga gaaggtacga tcggcggcgg cggcttcttc
    15121 tatcgtaatc tttaacagag tgcgagtctc cggattcatc gttgtgttcc acaactgttc
    15181 tggattcatt tcaccaagtc ccttataacg ctgaatatct atcttggcag tcgaatcctt
    15241 cgaaaatctc ttggtcaaga cttcaagctc ttcatcatcg taagcgtaat attcttgctt
    15301 tttacataca actttgtaaa gcggtggttg agcgatatag atatgtccat cctctattag
    15361 tttgggtaga taacggaaga aaaaggtcaa cagcagggtg cgtatgtggc taccatcaac
    15421 atcggcatcg gtcatgatga tgactttgtt atatcgtaac ttttggatat cgaattcatc
    15481 gtcaccaaaa ccggttccaa gagctgtaat aatagtacgg atttcctcat tgctcaaaac
    15541 tctgtcaata cgggtttttt caacgttgat aattttgcct cgcaacggca gaatagcttg
    15601 atagcgccga tcgcgcccct gtttggcact accccccgcg gaatcacctt cgaccagaaa
    15661 taattcgcac attgatggat ctctggtgga acaatcagcc agttttccag gaagattgcc
    15721 gatatccagc gcactcttac ggcgcgtcaa ttcgcgggct ttacgggcgg cttcacggga
    15781 acgagccgca ttaagacttt tctcgataat acgacgcccc tgagtgggat tacgttccag
    15841 atattccgcc aaactttcgg agacgatcga gtccacaatg cccttaactt ccgaatttcc
    15901 gagcttagtc ttggtttgcc cttcgaattg gggttcaggc accttaacgc taatcacagc
    15961 cgtcaaacct tcgcgtacgt catcgccaga caactgaaat gactcttttt tgaaaagatt
    16021 gtttttctgg gcatagttgt tcagggtgcg agtcaaagcc gtcttaaaac ctactaaatg
    16081 agttccacct tcaattgtgt taatattatt tacaaaagtg aagatattct cattataaga
    16141 agtgttgtat tgcattgcaa attcaacgaa aacgccgtcc tcttcttttt ccgcggttat
    16201 cggtttatcg ataatagccg gccgggtttc atccaaaaaa tttacaaatt cagctagccc
    16261 gcctttataa tggaatctat tctcgttttt atcacgctca tcaaccaaaa cgatttctaa
    16321 atcccggttt agaaaagcaa gctcgcgaca acgatgctcg agtacttccc attcatactc
    16381 tattttcttg aatacttcaa catcggcttt aaaccttacg agcgtcccat gtttattagt
    16441 cttgccaact ggttcgactt tactggtggg taccccacgt gaataactct gagtataaat
    16501 cttgccgtcg cgatatactt cgaccctcat ccattctgaa agcgcgttga ccaccgaaac
    16561 gccgacaccg tgcagtccac cggaaacctt ataggaacct ttatcaaatt tacctccggc
    16621 atgcagtatg gtcatcacga cctcgagggc tgatttcttt tcttctttat gaatatcaat
    16681 cggaatgccg cgcccattgt ccagtacgct aacggaatta tcttcatgaa cagttaccac
    16741 aatcttatta caaaaaccgg ctaatgcctc atcaatcgag ttatcaataa cctcaaatac
    16801 aaggtggtgc aaaccgcgtt ttccggtgtc accaatgtac atagccggtc gctttcggac
    16861 tgcatccaaa cctttaagaa ctgtaatttt ttcagccccg taatcaattg cggcgtcggt
    16921 aaatttaatt tcgctcacct taagataatc tccttaatct gtgcattttt tatttgactg
    16981 tttatccgct ttaatatttc ttccttgcga aagtttaatt cattgcgcca aaccggcgaa
    17041 tcaactttga caaataattt attataggca actttttctg gtatggcatg cttggcaatg
    17101 acttcaccca caatgtcttg ccagataaaa aaaacttcat tctgacgaac cggcttttct
    17161 atgccataaa tttttagcat tctggcaata gccgtaccga gagactccat ctttatgctt
    17221 ccccagtagt cagattaaaa attttatatt tttcaggatt atcaagaaaa ccatactttc
    17281 gcaacatttt gacatcggtt gaagtaatca gcgcctgatt tttatcaccg atttcttcaa
    17341 cgattttacg acagtgttta ccatctaaaa aggcaaagag atcatctaat aaaaatataa
    17401 ttttttcgtt ttggtggctc tcaatatacg ctccttcagc caattttaag gctataagaa
    17461 ttaacttatg ctcaccctga gagccgatct gacgaatatc tctgccgtta taattgataa
    17521 taatttcatc taaatgaggt ccgcaggttg ttcgcccgat aagtaagtcg cggctgaatc
    17581 tttctcttaa gtttttggta aacaaagaaa taaaattgtc cgggtcggct ttaatattat
    17641 atttcagatc aattcctacc gttccgccaa tatgggtaat tcgcgcaaag actgttttga
    17701 aaatcgagtc catatcggca ataaatgcct gtcgtccaat aaacaacttt tgccccagtc
    17761 tggctaaaat ttcgtcttgg gcttcaaaat aagcatcata acgacagcgt tttttctctc
    17821 gatactggtt gagtacgaca tttctttgtg aaattctacg gcgatattct atcaaagtta
    17881 ccaggtaatt gcgatcggtt tgcgaaatta tccgattaat gaaattacga cgttcggctg
    17941 ggccgccttc cgtaattttc tgtaatcctg gtgataatat cactactggc gcacaaccga
    18001 cgacgtcggt gtatcttttt accctatttt tattcatcaa aaaactgcgg ccttcagttt
    18061 tagaataatt catattagac aaaattgtct gattgttatc gcttaaaaac tcaccaaaaa
    18121 tattgaagta atcactttga taattaaccg cttcgctatc attattcgta cgaaaacttt
    18181 tggttaggga taaataatgg atggcttcga gtagggaagt ttttccacta ccattatctc
    18241 catttataaa atttgtcttt tcgtgaaata caaaaacgga atcggtatat tttctaaaat
    18301 ttacaagtct caaatttttc aataccatta ataatcatct tttacggaaa ataaagagag
    18361 gtgttaatca cttagcctga tcggcattaa aagcattatt aaatcttcat tttcttcttg
    18421 attctccggg attaccaggc tggcgctgac cggagtcttt aatttcatta taactttttg
    18481 agtatcaata ttcctaacga tttctcgaag atattcggca ttgaatccaa ttgtaagatc
    18541 gtcgccacta tattcgacat cgaggatttc atctgccgac gacattgttt ctaaatctat
    18601 cgtgcttatt ttagatagtt ctcgattcaa actaagcgat atttgatggg ttgatttatt
    18661 agagaatata gacactcttt tcaaaaccgc tgaaagataa tctgaatcgg ctttcagctc
    18721 cttatcatta tctttgggta taacactttc ataatccgga aaacgttcgt ctattattct
    18781 ggaaattatc agggttgaat cactttccat cattatgtga ttgtcgctta tagttaaact
    18841 aaccttatta tcatcggtaa ggtaggtatt aattagattg agaaatttaa ccgggactat
    18901 gatctgtcgt tcataatccg gcgaactgaa atcttttcta ttaatatata ccagccggtg
    18961 accatctgtc gctaccgatc taagttcgtc agctttgact tcaaaaagta cgccggtcaa
    19021 agacggttta agttcatctc tactaaccgc aaacactgtt ttttgaataa ttctcgataa
    19081 aattttactg ttaactaaaa tactattttg attaataata cttggcaaag ctggaaattc
    19141 ttcacctggc cttcccataa ttttatattt accttttcct gcagtaattt ttatctcgcc
    19201 ttcatcggta gcattaaagg ttagttcacc ctctctaatc tcacctacta tttcttgtat
    19261 gagtcgcgat ggaactgcaa cacttccatc ttcttgcccg ttcactgtaa aagtcgtact
    19321 taaggtaatt tcaatatcgg aagctcttag tgttaaatta gaatctttca agataaaaag
    19381 aatactattc aatatcggca tagttgttct ggcgggtgtc actttggcta ccttatttag
    19441 attattaagt agtaaatttt ggctaacaga aaactgcata ttctatctcc ttacttttaa
    19501 tattttcata acaagatttt atcaaagaat actgtttttt ccaagtaatt taaaacaaat
    19561 aatcaaaagt aatttttata tgttaagtaa ctttgaatta ttaaaatatt agtagttaaa
    19621 taatagatgt taatttgtta acaacattta aaaatacgat gattgagaag attattaatt
    19681 gtaaaataca ctgttgataa tttgttaacg ttcctaaaca ggagtaaagt atttgtcgtc
    19741 gtgatctaag gaaagactaa tgcaccttgt taacaaataa ttaaaatagt gttaacctgt
    19801 taataattta ccttgatgtc aataaagatg tcttctttta gcttatttag ccactcttga
    19861 tacagttgat tctttttaaa ttccagtgca aatctttcta tcgttggcca actatcatca
    19921 taaacgttat taagttttaa aatataatat ccgggtgacg actcaaaaac cggactaata
    19981 tctccaacct tcatattact taagacatta ataaaatctg tattttgaat ctgatttagc
    20041 ggaatgcgtc taatacgccc taaggtggat ttggcattag gttcgtcgct atattttact
    20101 acagcagaat cgaatgaaat ttcattattt ataatcatag tacggatact atcagcgaag
    20161 cgatatcggt cttcatagtt tttatcggac accttgattt tgatcaagat atgacgaacc
    20221 ctaatgcttt caccttttcg gtcaaccatt tggatgatat gatagccgaa atcggtttta
    20281 attacgtcgc taatctcacc gggcgagagt gaaaaagcca cctcttcaaa actcttaata
    20341 aatgcgccac gcttaatata ccccaagtct cctccataag gagcacttgc atcatcctcc
    20401 gaataatttt tagccagata ctcgaaatca tacccatttt ttagtaaagt atagagcgaa
    20461 tcaattaatt ttttagccgc catttcttcc tcgggaccag gagtgacttt gaataatatt
    20521 gcactaaaat ccagtgatgg aggaatcggg ggaatactgt ccttgtaggt ttgataaaaa
    20581 gcctccacct caggtcgtgt gacggttact ttactaaatt tttcggaacg cattttctcg
    20641 acgatcagct gatttttaaa aatcggacgg taatcttttt ttattttact gataggactg
    20701 cccatcaaca attcgagatt ttctaccgaa ccaacctgct caacgagcat atccaaacgt
    20761 tggttaatcg cattttccac atcgcgctct ttaacttcca ccgtttcaat tctggcttgt
    20821 tccagaataa cattctcatc aattaaagaa tttaaaacat gacgtttgat tttattaaaa
    20881 tttgcaacgt cgcggttagg atcaatattc atctgagaag ccgtgattcg cgccacctga
    20941 tcaacctcac ttatgaggat aatgttctcg ccgacaattg cggcgatacc gtcaatgagc
    21001 gtttgagcga aaagagtggt cgcaaaaaag ataataccaa aaaacaactt aattttcatc
    21061 gagagcctat agagtttaaa aaattggaaa tattttcatc ttttatttca taattagcat
    21121 tccggacaag actgtcaacc agtgcgttat actgatcttg gatttttttc tgagtcaata
    21181 tctgatagat ttcatcacga acctcatcga ttgttcgcgt tgagccgata ggatagcggg
    21241 ctagaacctc aacgatatga aaaccataat ttgatgcaat gggaccaatg atcgaacgag
    21301 gagaagtttc aaaaatatta cgatctattt cttccaaaga ttcgcctcgc ttaacgacag
    21361 aggtctcaaa tttatactta ctataaaacg catcaagcgc attcttattg cgactagtta
    21421 tagcagattt tattgccagc gcatcgttaa aatcctgcac aagaacatga acgatcttag
    21481 cttcctcacg atcgcgaata aaactttttt tatttttaag gtaataatca cgtatttcgt
    21541 cttcactaat agttacattg gtttgtaaga aaaaacgtac ataggaatct atagttaaat
    21601 cacgataata atccgccacc cgggcttgta cgccctcttc gcggtcaaaa tgatactggc
    21661 gagctttttg ataaagtatc tccttgttta tccagttgga aacaacagag tttaggtaat
    21721 ttttatcaat atcttttgat aagccaccgt cgggtaacat tcgggttaag tcttcattgg
    21781 ttaaataagc atttccgacg cgcgcaatat aatcttcttt atgattgcca caagccgccc
    21841 agagaaaaat gaatatcatt aataaaaagt ccttgcgcaa ttttatacct cccaagctaa
    21901 gttacctatc ataaacaaac taaaatctat gctttaacaa tcaaacggag atttagttct
    21961 agcccaaaac ctacattttt agaataaaaa tacattttca taacttttta cactcctaaa
    22021 aagatactat aagttaaggg tgttacgaag cagattcaag aagcgagtaa ttttatcaaa
    22081 atcaaaatta ctttttaaat aaacggttaa atataaggta ttgtaaggat taaacttata
    22141 agacaaaccc aaatcagaag cggccttttg gatggcttgg atgagatcgg tttcggctgg
    22201 cggtataaaa gattcgtccc agataaagct ggttgatttt tcggcaatat tgactttttt
    22261 tattccaagt gatgaagcga ggagccgaat cttgctgata ttaaaaagag tcgttgcctc
    22321 agacggtaac tttccataaa tatcgcggat ttgtcctgag attaaatcta ctgtagacaa
    22381 ttcatccgcc agtgttagtt ggcgataata ttctaaacgt gccgaagaat cggaaatata
    22441 actgtcagga aaataggctg gaagtgggca aaaaacttca gtttccaatc ttaatttttc
    22501 tgggtaagcg ggctgggttg atttgatctc atcaagcgcc tcgcgcaaaa atttcatata
    22561 gagctcatac ccgatcgccg ctatattatt gccgctttgc gaggtaccca gaaggtttcc
    22621 cgcaccgcgt atttcgagat cttgaagcgc cacactataa ccggaaccga gtgcggtatg
    22681 ttttttaata gtctggagtt ttctaagggc gtccggttta atgcgagacg gttcgggaac
    22741 aatcaagtaa gcgtaagccc gacgactact cctgccaacc cgaccgcgta attgatataa
    22801 ttgagccaga ccaaaatgat gggcttgatt aataaaaatt gtattagcgt taggaatatc
    22861 aatgcctgat tctataatgg tagtggtaac caataaattg atttcattgt taatgaaagc
    22921 cgacatagtc ttttcgagtt ctctttcatt catttgacca tggacataat ctacgctgat
    22981 ttctggtaga gccagagcaa gtttatgact gagagcggca atagatttga tgtcgttatg
    23041 cacaaaaaag acctgaccac cacgattaat ctcccgcaag atagcctttt taataatatt
    23101 catatcgaaa gtgatgactt cggttattac tggcaagcga gagattggcg gggtgttgat
    23161 aacagaaaag tcgcgtactc caatcaaaga aaaatttaaa gaacgcggga tcggggtagc
    23221 cgataaagcc agaatatcta ccgattcgcg taaaaattta attcgttctt tgtctttgac
    23281 gccaaagcga tgttcttcat ctataactaa taaaccaaga tctttaaaac taacatcttt
    23341 tgacagtagt cggtgagtac caatgacaat atcaatcttt cccgcggcaa gatcacgaat
    23401 aatctgtgac tgcattttgc gggatacgaa tcgcgataaa agtgcaattc ttatcggata
    23461 tttttgaagc cgctggctga aaacagcaaa gtgttgatca gccagaatag tggttggtac
    23521 caataccgct acctgcttgg acgcggatac cgccttgaaa gaagcccgga tggctacttc
    23581 ggtttttcca aaacccacgt cgccacacaa aagccgatcc attggacgag gtttttccat
    23641 atcctcttta acggcttgaa tagcagtcag ctggtcggga gtttcttcat ataaaaattc
    23701 cgattccatt aagtattgca gttcattgtc tggtggaaaa gcatagccgg gcgcgcttat
    23761 ccttttggcg taaagttcga ctagatcttt cgaaatctca tcaatagctc gttgtgtttt
    23821 taatttggtg cgttcccatt ccgtcgtacc aagtttgttg agaggtggcg gattctgaag
    23881 atttccggta tatttctgaa catagttaag cttctcaagt ggtacaaata ctttatctcc
    23941 gccagaatat tccaggcaaa gacattcacg gatcgaacca aaagccgcga ttttatccaa
    24001 tccagcaaag cggccaaccc cataattaat atgaaccatc aaatcgccgg gagatatttc
    24061 gtgcggactg aattgttcaa caggaaagtc tggggaatac tttttgaaaa aatttatccg
    24121 tcgttcgcgc ttgaaaattt ggtgatcggt ataaaaatac aactttgctt cgggtagttc
    24181 tgcccccgcc gagatagaaa aatcaaagta gttcaatttt tccaaaccca gtaattcctt
    24241 aagacgttcc acctgcatag aattagcaca aaacaacgag attcgggcat ctggctgggc
    24301 agagagcact gtcaataaat gttctctaaa agctttaatt gaattggttt gcggttgagg
    24361 attcacccgg aaatcaacat ctgaagcaag taagtcgtaa tgattaatta ccctgtatga
    24421 tgatagataa gtaaatatat cagtcgtgtt tttttcatct gtatccataa gtttttcagc
    24481 atgaaggcaa aaaaccacgg tgtctttttt aaggtaagaa aaaatattag tagagttttg
    24541 attatctagc gatgaatgcg ccggagcttg tactgtgaag gatgatagat tgcaaatcga
    24601 gagctgggtg tcaggattaa aagtgcgaat ggactcgatc ttgtccccga aaaattctat
    24661 tcgataggga tgcagagatt ctggaggata aaaatcaata attccaccct tgatgcagta
    24721 ttcaaatgga ctttccactg ctggtgctat ggtataaccg agatgaacaa gatattcgat
    24781 aaaagcgtcg cgattgacta tttcgttgac gtgataggtg cgtttaagct tattgaaatc
    24841 atcagaaagc gggaatttac ttttaagcga agataaagta gctacaatga tcgtattttg
    24901 tccggtggat agtttttgaa gggtatcgtt agtgaagtaa gcattgagcg attccgtatc
    24961 agaaaatagg aaggaagaat tcggcggctc cggcagatag cataccgagt cgtcgagatt
    25021 aaacagctcc aaatcatctc ttatgtattc cgcttcattg gaatcattgg taattaataa
    25081 aattggctgg tcaatttttt tataaaaaga atagagaaaa agcgcagttg caaaaccgga
    25141 taatcccgca acggtaaacc cagcgttaga gcgataaaaa gccttaacgg cattaagaaa
    25201 agctgaactt tcggaaaagg tagagactaa attttctata cggtcgtcta aatgtttcac
    25261 gttaaaccag aatgcttatg aaggaggaaa aacagcgccg cgacgtctgc tggagagaca
    25321 cccggaattc gagaagcctg tccaagtgtt tgcgggcgga agcgaatcag cttttcacgc
    25381 gcctcgttag ttaaagatcg aaccgcagaa taatcgaaat cagggggaat cttaatattt
    25441 tcattttttg acattgattc gattaaggct tgactgtgtt taatataacc agcatatttg
    25501 acgtcacact caatctcctc aataatcttg ggaaagtgag caatcttatt ttttatttca
    25561 gatgataaaa aaggaacgag cgaagcgagc gcaacatccg gacggcaaag caatttatat
    25621 aaagagagat tacagccaat ggaatgggcg tcattattag gtaatatgac tttagccgct
    25681 tcgggagaaa tatttgttga agataaaaag gatataatct cctcaataac ttctatctta
    25741 tgttgggttg attcgtaagt ctctcgcggc aatagaccga cttcatatcc ttttcgggca
    25801 agtcggcgat cggcattatc aaaacgtagc atcaagcggt gctcggcgct cgaggtaaac
    25861 attcggtagg gctcattggg acttttggta attaaatcat caatgagaac cccgatatag
    25921 gcttcgtgcc tttgcaaaac aaatggttcg cgaccttgga tggaaagggc ggcgttgata
    25981 ccggccatta atcccagagc ggccgcttct tcgtagccgg aagtgccatt aatttggccc
    26041 gctatataaa gtccgttgag tgacttggtt tcgagagtat ctttaatttg gtaagaaggg
    26101 aaaaagtcgt actcaacggc atagcccggc ttgataaact ccgctttttc gagacctggc
    26161 actgttctta atgccttcat ttgaacgtca attggcaggc tggtcgaaaa accgtttacg
    26221 taatattcgt tgctattttt ccactccggt tctaaaaata tttggtgaga tgtgcgctct
    26281 ttaaagcgaa cacacttgtc ctctatgctg ggacaataac gcggaccaat tcctttaatt
    26341 atgccagaat aaagcggtga acgatccaga ttgttcaaaa tgaaattgtg ggtaacggtg
    26401 tttgtgcggg ttaaaaaaca aggaatatta ggtggcgaga aattttgggt gctaaaagaa
    26461 aacggaatcg gatcttgatc tccatattgg ggcattgtcc tagaaaaatc tatagattcg
    26521 gccaagactc tgggtggcgt gccggtcttc agacgtccag aaaggatatt atatctggca
    26581 aggtcttctg taaggccgga gaccggttct tcatcaattc gcccgccatg gaattgttgt
    26641 agtccgacgt ggattaatcc attgaggaaa gtgccgcaag ttaaaattac ggcctggcaa
    26701 aatatgtcgc cgttatgaat taaatgaatg cctttaatag tctgcttatc aactatcaag
    26761 ctattggcag tatcttcgag aagggtgagc cggtcttgat ttgataaagt gttcctcgcc
    26821 aacaacgagt agtgaatttt atcggcttgg gcacgcggcg accaaaccgc cctgccctta
    26881 gacttgttta gcattttgaa ttggattcca gtccgatcga ttaattttgc cattgcgcca
    26941 ccgagagcat caatttcagc tacgagatgg ctttttgcta aacctccgat cgatggatta
    27001 cacgacattc taccaatggt agaaatattt tgggtaacca gtaaagttga aagtcccaaa
    27061 cgagctgccg ccaacgccgc ttctattccg gcgtgtccgc ctcccacaac cgtgatgtca
    27121 aagtctaaat gtttcatgtg aaactactat ttgccgatgc aaaaatgctg gaaaatattg
    27181 tttaatattt catcattaga ggtttttccc agaattttgt catattcgtt gagcgctatc
    27241 cgtagatcgg cgatgatcat ctccggagct agactttgcc taatgtgttt ctgggcagaa
    27301 agtaaatatt tttttgccgt cctcagaccg gtagcatggc gctgattggt gatgatgagg
    27361 ctgtcggcgg tgccaatttg ttgcgagatt gaatcaacga tcatctcggt taatttgtgt
    27421 attccctggc gtttctttgc cgatataaat atatggtgat attgagagaa atgcttttca
    27481 tactttgtca ggcttttttg gggggcgata tcaattttat tgatcacaac gatagtattt
    27541 ttagaattgt aatattctaa ccattctctt ttaatcggtt gagtagcatc aataacgaat
    27601 aagataatat cgcctgtctt gatatttttc ttagtgcggt caatgcctat tttttctatt
    27661 gggtttttgg ttttgcggat accggccgtg tcaagtagtc taatcatata gccaccggct
    27721 tggattgctt cttcgagtga atctctagtc gttcccggat aaggcgtaac gattgcgcgt
    27781 tcttcgttta aaaaggcatt taacaagctc gactttccgc tattaggcgg cccaataatt
    27841 ggcaccaagg cgccttgctt tagtatttta ccgcaagaat aggattttag tagtttgctg
    27901 ttaattttca atatgttatc aattgatttt gacaattctt gcggtggaat cgtatctatt
    27961 tcatgatctg aaaagtctaa ttctaattca agaatagtta ttagctcgat gattttactc
    28021 ctaagttcag cgaattcctt tccgattgtc ccttccagtg atttaattgc ggcttggtga
    28081 gacgcttggg tttgagagaa aactaaatcg gctacggctt cggcttgagt cagatcgaac
    28141 ttaccgttga gaaacgcgcg tcgggtaaat tctccgggtt gagccaatct ggcaccatac
    28201 ctgaggcata attcaatcag ttggttgaca atgaaagggc ttccgtgaca gctgatttcg
    28261 actaaatctt cgccggtata ggacattggg gctttataat agattatcac agcatcatca
    28321 agcaactcgc cgtttttgta taaattgaga tgctgagcaa ctcgtggttt tagttgggtg
    28381 gtggttttgg ttaatttatg aacaatatcc ggtgattttg gaccggaaag acgtaccact
    28441 gccagcgctc cacgcccggg gggagttgaa agggcaacta ttgtattttc ggttgacatt
    28501 attctcagta gaattcattc agcgaccgcg catccttgtt tggtattggc gtagtctttg
    28561 agaattggtt ttatggggtt tcttgggttt tggtttgggt ggaaaatatt tttgctgaat
    28621 aatcgaaaga atattataaa gagtatagta caaatttaaa ccggacggaa actgattaaa
    28681 taaaaagaaa aagaagatag tcataaagta catcattact ttctgctgtt tgccgccaga
    28741 agcaccggat attttttgct gaaccagctg cgaaattgcc ataataaaag gcaaaacatt
    28801 tacctgacta ccataaattg gaattgaaaa tggtaaggtg aaaattgtgt ccggtgcgga
    28861 taaatcggtg atccaaagaa taaaaggtgc ctgccgcaat tctatcgtgg tgcgaaaaac
    28921 tatgaaaagc gcccatagga tcggcatctg aagcagaagt ggcaaacagc ctcccatggg
    28981 attgacacca tgctcgcgca tcaacttaag gcgttcatta ttaaggcgtt gaggatcgga
    29041 agcgtatttt tcctgaagag cgtttaactg cggttgaata ttttgcattt ctttcattga
    29101 gcgagtggaa ctgtttgtta aaggggtgag gacgattttt aacaaaacag aaaaaatgat
    29161 taatacccag ccatagttag gaataaaaag gtgcatcttg gtgaacagcc ataagattcc
    29221 tttactgata ggcctaatta aactaaaccc aaagttcatc aagtgttcga aattaggagc
    29281 caagccttta attatgctat agtcaagtgg tcccagataa atctttaccg cattttctga
    29341 ggaagcgttc aaagtcagat acatattgaa tattttttgg aagtctttgc cttgtaatgg
    29401 aacccctaag ccagttaggc gataaccttc agcgggctga tgaggtatca aggcggcgac
    29461 aaaatacttg gtttttatag ctgtccaata ggttgcaccc accaagctag ctgatccagt
    29521 tttacctggt tttacatcga gattttcggt ttcgccaccc ccgcaggcgg aagcccgtga
    29581 ataggagacg tcttctttga tcatagcttc agtataggcg attccgcctt cccagaccag
    29641 atcatattta tcagaagcca tttcatcgag gattccaccg agatcattgt acgagtttat
    29701 aatgtattta tcaccgtaaa agacaaaggt tctcgaaacg ataatattat cgccctctgg
    29761 caattgaaac tgaataataa tactatcgcc atcggaaatt ttataatagt ttttactggt
    29821 ttcttgatgt tcggtaaaaa ttctaaaatt ttgatcaatg gtcaccgaat ccccattact
    29881 ttttatgtat ttaataaaaa gccgccttgc ctcaaaaggc gggatcattt cgaccagact
    29941 tgtatcgcgt ccgataattt tattgtagtt ttttaagacg acgctggtga tagtgccgcc
    30001 tccacgcgag gatattttaa gacgataaag atcattttcg attgtgagcg tgtcaggttt
    30061 ttcacttcct aaagaagccg ggcgttgcac ttcgcttgag ggatgggatt cggataattg
    30121 cgctggaatc gtttcagaaa taacgccttc tggtgttttt tcaatgactt tctcttgagt
    30181 gacctcgggt gaatttttcg aaattatttt aaaatagtag ggcattgtca aaattataac
    30241 aaatatgaga acaaagccga ttagtgattt tctatccatt tttattcctt tggagttggg
    30301 aggggatcat aacctcctgt atgataagga tggcatttaa gtattctggt gagcgaaagc
    30361 caagaagcct taaaaaagtt gaatttctta aaggcttcta aagcatattg agaacaagtt
    30421 ggcgtaaaac ggcaagtgtt gggaaaaagg ggcgagagaa gcaagcgata tgtttttata
    30481 attaaaataa tcaaggtgtt aaatattttc attccgttta taaatttagg ttgtttatca
    30541 gaatttctga taatgtgttt acctgcaaat tatcaggttt tttaaaaaca gtaatggcga
    30601 ttgccgccgg tcgtctacaa taaggtttgc agagacggaa agcctccctg attttacgtt
    30661 tgtataaatt cctttgaacg gcattcccgc attctttttt tatcacgatt gataatttcc
    30721 aatcatcttg gggtaagatg gaaatgtaaa ggcaatcata ttttagcggt tttccattat
    30781 gaatgatttg ggcgcgttga tcccatttgg cgagccgttc acgacggggc aaattaggcc
    30841 gataggacct tacgtccttt agctcgtctt ctggcgatga cttttctgcc actattggtg
    30901 ctcatccttt ttctaaaacc gtgatcgttt tttcttttac ggttgctggg ctgataagtt
    30961 cttttcatat ttcctcattg ttaattaaaa acccgcctaa tttaactttt ttgaccttat
    31021 tatcaatgtt agaaataaaa tgtgg
//
LOCUS       DAOMJU010000015        29884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1361_length_29884_cov_16.617285, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000015 DAOMJU010000000
VERSION     DAOMJU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 29884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29884
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1361_length_29884_cov_16.617285"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..495)
                     /locus_tag="PLO58_02685"
     CDS             complement(19..495)
                     /locus_tag="PLO58_02685"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HQO73727.1"
                     /translation="MNTLTKKIIPVNPVNFAKFGQVVALPREKPTAEGDTFKFWANIA
                     NYRINGKTEIGLCTVTLPRGNRVNSLECHLHTPEILIPVDAPFALPLLIEGEPEENLK
                     IFRVNVGEAVVINPGVWHGASIPLEKSEATYFVIFRYQTSQKDIEKKSINDIEILI"
     gene            complement(500..1945)
                     /locus_tag="PLO58_02690"
     CDS             complement(500..1945)
                     /locus_tag="PLO58_02690"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HQO73728.1"
                     /translation="MKKYYCLQSFLALIMLIIVDCSRPNSPNYQRLMKTKDAEFVHIP
                     VGLCEDYPEETTTMEIIYNDMELLKRTGVDLLRISFGWDAIEAQKDQYDWLFWDDYVK
                     TAVNQYGITLIPYICYTPLWNSRGDTLWFWNDQPLDFEEFGEFMYDLVTRYKKYIKTW
                     ELWNEPDLDIYWDTRDVAAFAEFIKIGAQAVRRADPEAKVVLGGMAYHADFLERLFRD
                     YGLSPYIDIVNMHNYYETWSERPVEKITENINAMAEVIKKYGDDQSLWMAEVGYSTFR
                     QGSYVSDSYYAYYDYEHTPEYQAVDLFKRLTLAVAEEKLSAIAWYEIKDLPAGEEVIG
                     DNYNNRYLGIAYADYRPKPAEKALVFFRKLFGNPYRNVTSLISVEHQPDSDVFYRAFE
                     KEDGTIIFVAWIATNVPSKRPLKPANLVQDTRTETISVTIPAKLKGRVVQYNELGSAS
                     TFNEFQRNDDQTRLNNIVLNGGEIKIFELEK"
     gene            complement(2044..2445)
                     /gene="rbsD"
                     /locus_tag="PLO58_02695"
     CDS             complement(2044..2445)
                     /gene="rbsD"
                     /locus_tag="PLO58_02695"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HQO73729.1"
                     /translation="MKKHGILNSHLSMVIASLGHTDQLVICDAGLPLPREAEIVDLAL
                     VPNVPRFIDTLKAVLEELIVEKAILAKEIESINNGILKEVKGLLEGIPIEYVTHEEFK
                     NIYRKSNNTVFVRTGETSPYANIILISGVDF"
     gene            complement(2462..3382)
                     /gene="rbsK"
                     /locus_tag="PLO58_02700"
     CDS             complement(2462..3382)
                     /gene="rbsK"
                     /locus_tag="PLO58_02700"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HQO73730.1"
                     /translation="MTKNSVIIVGSANIDLVAQVDRYPQPGETVFGKKFGIYPGGKGA
                     NQAVACAKLGADTIFIGKMGDDIFRDRLVNNMESNGVHMEEIFIDSETPTGVALIYVD
                     ADGQNEIVVVSGSNMRLTESDIRSKEKLFERAKIVLCQLEIPLSAVEAAAILAKKNGA
                     VFILNPAPACKLPASLLNGIDFLIPNEIELSFLTGQSVTDRASVISSSRKLINTGVGN
                     VITTRGENGAILVNSDSWQEFPATPVKAVDTTAAGDAFNGAFAAALASGKPVNEAIPF
                     ANKVAAWSVTKQGAQTSMPTLADISNYLNQ"
     gene            complement(3393..6656)
                     /locus_tag="PLO58_02705"
     CDS             complement(3393..6656)
                     /locus_tag="PLO58_02705"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQO73731.1"
                     /translation="MKLLNELWPDKELNCENFKNPPLEMGILPFWFWNGEMDEAEMEW
                     QLKEYKAKGITGLFIHGRFGLKIPYLSEKWFERVKFAVKKGREIGLNIWIYDEMNWPS
                     GTAERQVIKKYPYLSQKYLEMVTLFINGPLFIYLEATDSRYINTGDSKPIAAYICSAE
                     EYRTGIKNIIDVTPNLSFNKVIPWEAPAGNWVLMYFLEKEIDYYIDALNPEATKRFIE
                     LTHEQYKTAVGAEFGKVVPGFYTDEPAMHYFQVGTDNYVIPWSQRMFKIFRERNGYSI
                     KPHLAALYANVGRDTARIRYDFWKTLSDQYDDSYYKQLRDWCAENGVLFTGHILFENW
                     LRGHARCEGNVMQHLSNLHLTGVDHLYPKIGSEEQPGEHVELKMASSITHHLGKKRLL
                     CESMGGTYWDCTLERMKWIANWEYSLGVNLFNNHGYHYSIEGERKRDWPPSQFYHHTW
                     WKYYNEFTRYMARLGHALTGGKHIAKILILYPITSIWANYVPQAPDDISRLIEMDFNY
                     LTDALLHLHYDFDYVDEDILINAQLNGNRLEIADESYELIILPPVTHIKKATLDKLKA
                     FVNNGGKLIADILLPVEQFIDGKPSKSRAVDELFGVKPNCILADFQNGKNAVNQLKML
                     QPGVCLIQGGGLAKDRRRQDIKEAIESCISADVQIGHSEVFYLHRVKDGRHLFYFVNT
                     LQQDIGEVDISLETVGRPELWDPATGAVKELSVFDIINNRLCFRLTFGASQTFLVILH
                     NDLPDSYISATNLVLEKTEANFITGYIKNGDLAKLTLKTGGKQKDIVQLVPKNPPPLI
                     LPEVMNFKIENDNTLCLAKWKMLVSNEFSNDNYAALDYDDDHWLPVTIGAWEMQLPEE
                     PDDIHYPQTLWYRIPFQIEELPNRVKLLIDGFSGSSFRLFINGTEVKDTGQRSQLDAE
                     IREINIHSYVHPGRNLIAVQLVVQRRTDGILDLIKITGDFALKLNDDGYSIVLPKKEV
                     NIGDWCQQGYPYFSGTGTYSTELTIPADFCKGKLFLDIECGEDVLELQINDSGPIIVP
                     WHPYQIDITDLVRPGKNTFRFSITNTLINTLEAVPKSSGLLKPPVITNYQLLKIKID"
     gene            complement(6886..7887)
                     /locus_tag="PLO58_02710"
     CDS             complement(6886..7887)
                     /locus_tag="PLO58_02710"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO73732.1"
                     /translation="MMLHNRKIKIVTLSAMIFFATNLRVEGFNKAGRTSLQFLKIGIG
                     ARSSAMGEACIANLNSIDAVFWNPAAITQMQRLESSFSYTKWIGDLNVTAGAVGLTID
                     RLGSIALDYIAMDYGDIPEALVTSPDGKLDTRTGLNFSGGDLSVGLSFSKKFTDRLSI
                     GIRTKYLREDLYTYSTSLWAFDVGTYYNTGWKGIRLAMTAQNFASQARWLKTQQREEQ
                     QYEVPLVFRIGTSIDLLGGKDLFLGGNPNKQRLSLNIDAIHSNDYAERLNIGAEYWLW
                     NRIALRTGYRTNREEGHFSVGAALNIPMDFTNLVVDYSFVDYDYLNSAHQFTVMLRF"
     gene            complement(7929..10136)
                     /locus_tag="PLO58_02715"
     CDS             complement(7929..10136)
                     /locus_tag="PLO58_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73733.1"
                     /translation="MKVKNYYIHLVIILGLLTGWSESFAQRKSTHTRGKLWETLNNVG
                     LIGDLGVWDYEETSGLGFYPGFSGYSFPNNEQLANGWVTDANFHNFRSGPALVIKGGD
                     VLVAPDYDPQYQEYLFYHSSFAGGSHGILWNYPPFIKTNNFVGTSQFNPLLPEEINYC
                     EFPTATGISVKMRSMAWSFPDYDDFIVYDYVFINTGEMVIPALGEVKHYEQTFDSVFV
                     VFHSGIQVSTKGHLNFHYDPDFMSSAAPAGGFGGWKQLENGGTYTDYFAVENDGDDSK
                     GLFFYSRDFNGGREPHPNDTYSKRSDWQTRLKYNQSYENIELQDPACFGFVFLYRTPP
                     TDSDADPFEADPNYFNIYSDEGEQFQGRALDMNEYFSIERLGEKGMFDFITHNRIPEE
                     NNGDMYCFYTATFGPYYLAPGDSVRLILAEVAGSLDYHEVIRGDPNHYYPDSSIADIR
                     RNAEAARRALKWGFGATVEGINLAADVPESPPAPNCSATNASQGSDTAIIAIQWDKLA
                     EETVITDGAGNPFYDGSTDLSGYRIFRGIDKRGIWDEVVDIPIEEANRYWNAEIGKYV
                     YLDKDLQFGFDFRYYVQAYYSNPKTWVSANHTIVDNLGELVSDDYNMTPLTGARPGPV
                     DITAGWDVFVAPNPYIEGDPERSFGDPTPYKIEFRNLPERATIKIFTVSGDLVKTIEH
                     GPDTYGNLFGSVAWDQRTDSGLRVAPGLYIYAIESHAEGTKGKRTIGKLMIIR"
     gene            complement(10154..13021)
                     /locus_tag="PLO58_02720"
     CDS             complement(10154..13021)
                     /locus_tag="PLO58_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO73734.1"
                     /translation="MISKKAYLQIRRLISRTFLGFMLLISLGYAASDGKIVGKVIDKK
                     TGNPLPGVNIIIIGTEMGAATDMEGDYYIINVAPGIYSLQASMIGYKQVIKTEVNVSV
                     NHTTRVDFELEETVLEFGEAVTVVAERPLIELDKTSSRVVVEETDIKSRPVSQLSDIL
                     TSMPGIDYNPYGELTVRMGTLDQVAFMIDGMRARNPLNSQPYFNVNLMAIEELEIITG
                     GFDAEYGEAQSGVFNIITKEGPDHYVGAVEFRWIPPGLYHWGTALYDYSTERYWENTH
                     ARHLQWWIDNPDMWVDPNGRYGNDPSCSWTPEQAYQFYIDTHQPLTDYTNRSGYQTEF
                     SFGGPTPIKNLRFFLSGRYRTAPPITGNAYRKMGSWLDGTYKISYSLGGNKLLVFSGY
                     YNQDNTCHGMESMEYEGDYGNKYAYYDEAGYPVYRRNGQTLKFVHNLDKNTFYELHLS
                     RVFNYESQTKFPSDTADWTTSDIRRDYLRAYDSQGFAIPDAWAEIYGLHYIGYWYRKE
                     DRNTDLTLTGKLTRQINKNWQMRSGFGFTYYTFDRYQEARAWEAVEDKIYHPYEGNIW
                     WSNKLEFEGLIMNLGLRYDFYNPNDVIYLDPYDPFDIITAEMEGREPNPKTKPTYTFG
                     QLSPRIGVSHPITEKSLLHFNYGHFFQRASFGEYGEGEQTYSILSTYIVDPEFGSIEP
                     RNLGNRELRPRKTVAYELGIEQDFGPVLADVTAFYKDRTNDVRSITVITYNEQQYHTS
                     GNSDYADAKGVEFTLRRPLRGFWGGYLTYTWETGIVGQSGDPFTIYPPNVTVHASQDF
                     DIGDQLYYMRPSLSANLTLAAPSNFSPLGGVLANTQFSIDYYVSYPHKQISSDVYQGA
                     VNIIRPANKNANIRVRKEFKGFKGTRPAIFIEMQNAFNDKWHNVDLMEGDISAEDQVR
                     YINSNLMVYPEKMTNGAPFPDYAKYTNLPRKITVGMALSFQ"
     gene            complement(13090..14040)
                     /locus_tag="PLO58_02725"
     CDS             complement(13090..14040)
                     /locus_tag="PLO58_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQO73735.1"
                     /translation="MRIAALYGAHDFRIETKASPDIKDDECLVRVRACGVCHSEIHQW
                     AKTLDNLEYPRYIGHEVSGEILKCGSAVKGFKRGDRVAVWTDRAGYAEEVAVRANQIF
                     PIADQISFAEAMAEPIACTTNGVIKAGVELGDTVVLVGTGFMGLILMQQIALKGASRI
                     IGVDVRAEILDKAKMLGADLVINPQKEDVPAIIWDMTNGRGADVVFEVGGNQASLDLA
                     VDLLRMEGKLVIFGYHPGQRIIKDLGYWNWMAFNIVNAHFRDMATILRGAEIGIKLLN
                     EQKINMKPLITHKFKLDEIEAAFCAAQEKPKGFIKAVIEP"
     gene            complement(14130..16103)
                     /locus_tag="PLO58_02730"
     CDS             complement(14130..16103)
                     /locus_tag="PLO58_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO73736.1"
                     /translation="MRKVNMCLLALLLLVAVQSVALSQEEIVRLELNVPKVSPADVVI
                     DGVMDESVWGNAAEVNLVTETGYNIWASSYGREIAEPEYDELYGRLLWTDDTLYVFMH
                     IDEFVNDTTDLYWGGQFLDPTDTSGHWASDQLFIGVSNRLGVESWDNWEGNPMAAPDG
                     PYHLMIMGDRVTFNDGYPVYVPELDSAVIFDPSTFIRSAAQWNKETGVWDVELAIYNP
                     NVKASASVGFNVGGSFSSTYSRETTGDSYGYYTWQPNVPNEPFADPGDLGWCATYIQQ
                     NSEYWALLNFLPGPDDVIVRLELNVPKVSPADVVIDGVMDESVWGNAAEVNLVTETGY
                     NIWASSYGREIAEPEYDELYGRLLWTDDTLYVFMHIDEFVNDTTDLYWGGQFLDPTDT
                     SGHWASDQLFIGVSNRLGVESWDNWEGNPMAAPDGPYHLMIMGDRVTFNDGYPVYVPE
                     LDSAVIFDPSTFIRSAAQWNKETGVWDVELAIYNPNVKASASVGFNVGGSFSSTYSRE
                     TTGDSYGYYTWQPNVPNEPFADPGDLGWCATYIQQNSEYWALLNFGTGLNGIDVAEVN
                     EKPVRFALRQNYPNPFNPTTTIRFEVNERVPITLKVYNVLGKEVATLIDNRTYSPGVY
                     SVIWDGSSLSSGIYFYQLVTDKVVSTRKMILMK"
     gene            complement(16217..17518)
                     /locus_tag="PLO58_02735"
     CDS             complement(16217..17518)
                     /locus_tag="PLO58_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQO73737.1"
                     /translation="MRKKIIFWTISMVLILSDFTKAGDASTAPITSAKSLALGGYNYA
                     GLDGITTTFQNPGKILSTPGFGLAMNIIDRAGQQQCQKGSRDIHRSYLFNDFSGSAGI
                     YWVPNQKFGLAMVYNRALDYSVRWPLALMFTENGITRLYGFQLKSRLIADAYSQVFGL
                     QVGKMSLGAAINIYQIYQQFEFPISNPEWQTNRTNPVYALNLEQEDNAIGWSLGFILP
                     ITTTIKVGGSIRSGVDFDLEGNAKTDCFYALDSLAKKSRLHSRFQMPMTGGIGILYKP
                     TDQLNLNIDLSSEFWGSTQSKLDFNYSDTAWSAIIAAAPRDSITGYWRSGMPFYDRNS
                     FVFAIGLEYSGENNLKYRVGYNYQTTPNSDRSYSLLLPNVNQHRISAGIGFLFNQFYL
                     DLSIAYSVGVAKDIKKSQDEYFYGKYDTKTIIPSINVEYQL"
     gene            complement(17552..18391)
                     /locus_tag="PLO58_02740"
     CDS             complement(17552..18391)
                     /locus_tag="PLO58_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73738.1"
                     /translation="MNNIIIEQETFTPKFTWLGGYGCAFGVNRGDQARLDSTLQWLIK
                     VDGDNLHYPVKYGELPEGVTDITNEYGGHFEALIEDSTYTFWVIKEAAWDAVVENAGK
                     VILMAELDTVTTIANEDTLWIDSYSHVQKSCWIDVYTNVDMASIKLRGGFAKITLIET
                     DTSNSPVITWEIIDSSVEDSLVAAVGICLGESYSPKKIVWEAWSVDTVGGQKVYGKNN
                     VIGQPLVIGSPIPGTKVFVEYPAKGLSRNKTYLFWLAGKDWNGVDHLRSTKYHSYITF
                     NTW"
     gene            complement(18578..19420)
                     /locus_tag="PLO58_02745"
     CDS             complement(18578..19420)
                     /locus_tag="PLO58_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQO73739.1"
                     /translation="MDIDFRKSSHIVKDIILHVIIYALALATIAPFIWMLLTSVKDIG
                     DIFVYPPKWLPSKFHFENYARAFQAAPFGRYYLNSLIVACTVTLGQLITCSMAAYAFA
                     RLQFKGRDVLFYIFLGTMMIPYQVTMIPSFMVLYWLGWVDSYQAMIVPGLASAFGTFL
                     LRQFFLTIPKELEEAAYIDGCGKFRVLWQIIIPLSKPALATLAIFTFMGTFNDFIWAL
                     IVVNSDHLRTVQLGLAIFRDRYITDWDLLMAGSVMATLPILIVFFFAQKYFIKGITLS
                     GIKG"
     gene            complement(19420..20352)
                     /locus_tag="PLO58_02750"
     CDS             complement(19420..20352)
                     /locus_tag="PLO58_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQO73740.1"
                     /translation="MLNKSTRVKKTIINRRDGNSKKNILTALGFLSPTLVIFCAFVLF
                     PVFFSFYLSFHKWNMFSSGREFLGLENYRRLFSDPEFWMVLKNTFIYTLGTVPLNMVI
                     ALLTAIILNRRIAGKRFLRTAIFTPVVISPVAAALIWRWIYDPNFGLLNYLMDLVGLP
                     SVNWLNEPKAAMVALIIMGVWKTFGTNMVLFSAGLQGIPDHYYEAAEIDGAGWWSKFW
                     NITMPMLSPTTFFIMIMSMISSFQVFDIVYVLTSGGPLGATKVMVFYIYENAFKFFEM
                     GYASAAAYVFFAILAVLTVLQVKFMQTRAGYEAV"
     gene            complement(20342..21733)
                     /locus_tag="PLO58_02755"
     CDS             complement(20342..21733)
                     /locus_tag="PLO58_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQO73741.1"
                     /translation="MQFKNIGNQHSPQKCANVFTANLRGWSRYFLLSFCLLLFIGCGK
                     KEATKDDKITITFWHSFVASTIPALEDLIEQYETENPNVKIKAQYVPTGDALIQKLIT
                     AIQSKTAPDISWIHSDFLADLVDADAIYEMDHFIKSNNGLSKSEIEDIYPALLQYSSL
                     RGKIYCIPMEATNLALIYNVDMVKEAGYQDGYAPSNWEELEEYAVRLSKDFNNDGKFD
                     RIGFFLPIFPAAGPLSGWMVWQWIPYLWQAGGYIIAEDQSKVLYNSEAGVKALTLWKN
                     IYDKLNLRTFTTDYDVSFASKLVAMAMDGPWNLPRFNRMLKNLNWSFAPLPKGPVKQA
                     TICGGEYLVIFKQSEHPDEAWSFVKWIIRPDIQARWAMKSGYLPVRRSVSEIPEFKKY
                     LIENPNFKVFVDQMEVAQIQRSMDYGALQITRHMAEAIEEATLGGGDPKAALDKAAAK
                     SNKVLQSYWQNAK"
     gene            complement(21759..22793)
                     /locus_tag="PLO58_02760"
     CDS             complement(21759..22793)
                     /locus_tag="PLO58_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQO73742.1"
                     /translation="MTATLEDVAKKSGYSVSTVSRVLNNKGKVNRIKDETADKIHKIA
                     QELNYVPNQLARGLRLRKTHAIGLVIPDISNPFFAHVSRIIQIESYKLGYTLIVCNTD
                     ENQDTEIEQIQLLRSKGVDGFIIMPVGLKSDHIQELIDDKIPLVLLDRCFDSIDTNSV
                     VVDNYTGSYKAVEYLIKNGHKRIAIIQGLPNTSTNNMRVKGYKNALIKYNIEIDENLI
                     VGNDYRRENGYIETKLLLKMSNPPTAIFSTSDLITLGTLDAIIEEKLNVPQDVSLISF
                     DDVDFAPYLFTPLTAIQQPRQIMGEMAVKLLYDDLKSKGRKEKTRIELKPVLNIRKSV
                     RNLNSVINPD"
     assembly_gap    23210..23309
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23363..25777)
                     /gene="leuS"
                     /locus_tag="PLO58_02765"
     CDS             complement(23363..25777)
                     /gene="leuS"
                     /locus_tag="PLO58_02765"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQO73743.1"
                     /translation="MYPFKEIELKWQRYWEDNKTFKTPDKSNKPKYYVLDMFPYPSGA
                     GLHVGHPEGYIASDIIARYKRKKGFNVLHPMGWDAFGLPAENYALQTGTHPKITTERN
                     INTFRRQIKSFGFSYDWDREINTTDPKYYKWTQWIFQQIYKMGLAYLAEVPVNWCPEL
                     KTVLANEEVINGKSERGGYPVIRIPMRQWMLKITAYADRLLEDLDLVDFPESIKEMQR
                     NWIGRSEGADIDFKIEGSDQTIKVFTTRPDTLFGATYMVLAPEHPILKTLVPPEYKKT
                     VEAYQQAAASKSDLDRTELAKEKTGVFTGLYAINPANNRRIPIWTSDYVLMSYGTGAI
                     MAVPGHDQRDWEFAKKFGLEIVEVISGGDITKEAYVGDGILVNSGFLNGLNVAEAKRR
                     IIEWLSEKGIGQSRVQYKLRDWIFSRQRYWGEPFPLYYDEDNNIHLIDESKLPLELPP
                     LEKYEPSGTGESPLANLPEWVDFIDPETGKHCRYDTNTMPQWAGSCWYYLRFIDPHND
                     EAFCDREKEKLWMPVDLYIGGAEHAVLHLLYARFWHKVLYDLGYVSTPEPFMKLVNQG
                     MILGEDGEKMSKSRGNVINPDEIIDTYGADTFRMYEMFMGPLTRVKPWNTSSIDGVFR
                     FLNRVWNLYVTEEGLISPDIKDITPDASTNSILHKTIFKITDDIENLRFNTAISQMMI
                     LVNEIAKLAVKPLSVMKTFLHLLNPFAPHISEHIWNLMGNDQSLAYLDWPNSDPSLII
                     DDLVTMAVQVNGKIRGTFDIERDSEDQACIDKALQIDNVQRYLEGHTIVKTVVVKNKI
                     VSIVLK"
     gene            complement(25805..27019)
                     /gene="serA"
                     /locus_tag="PLO58_02770"
                     /pseudo
     CDS             complement(25805..27019)
                     /gene="serA"
                     /locus_tag="PLO58_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(27120..27596)
                     /locus_tag="PLO58_02775"
     CDS             complement(27120..27596)
                     /locus_tag="PLO58_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HQO73744.1"
                     /translation="MIYPACREVKHLLSGEVRTFDCELIFLDEDFGILRYILEKPYVV
                     NNQILPKNTQTYAFYWESQPFTLYRWFAPQGGHLGDYFNIADRIHLSNLQFEWRDLIV
                     DVFISPQGEISLLDENELPADIDGNLARYIDQSTQFIIGNHRKILSELNSLTAKLF"
     gene            complement(27608..28168)
                     /locus_tag="PLO58_02780"
     CDS             complement(27608..28168)
                     /locus_tag="PLO58_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HQO73745.1"
                     /translation="MNLLALILIAIGLAMDAFAISITCGLAAQPHNKFKFLKVPLAFG
                     GFQALMPLIGWLAGQTLQRFLANFDHWVAFGLLVFIGIRMIYEAFSKKECTNAIDPLN
                     SHRLFGLSLATSIDALAVGITFAFLEINIVVPIIIIGVITFIISFTGIIIGKRIGHYL
                     GKKVEVVGGIILIAIGFKILIEHLVA"
     gene            complement(28169..29491)
                     /locus_tag="PLO58_02785"
     CDS             complement(28169..29491)
                     /locus_tag="PLO58_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQO73746.1"
                     /translation="MNADLFIIIGGLVIISLLVIIMAKVRKKTDQSGDAALEALKEKI
                     IALESQLNIFPELVQSKTADIVQKRFTEFTDNVSTKNREMIEKFGNFQVEITKSLGET
                     SKKQTEEFSQFKDTFKRAVSEDFEHLTKNVAEKLDAINNKVQENLSEGFKKTNETFTN
                     VIERLAKIDEAQKNIESLSTNVVSLQEILSDKKSRGIFGEVQLNQVLYAIFGEKNDKI
                     FQIQYTLSNDKTVDAILFLPEPIGNIPIDAKFTLDNYKRSIDKSLSEAERIAATKLFK
                     ADVKKQIDEIADKYILPGETSNQAIMFIPAEAVFAELYAYHDDLIEYSHSRRVWLTSP
                     TTFMAVLNTVQLVLRDIERRKYADIIQQEIMKLSEEFRRYRDRWNKLSAHIDTVQKDV
                     KDIHTTSNKISNSFEKIAKVELGEKSELPPEQEVKYLNVINEEESEQE"
     gene            complement(29504..>29884)
                     /locus_tag="PLO58_02790"
     CDS             complement(29504..>29884)
                     /locus_tag="PLO58_02790"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQO73747.1"
                     /translation="AIVVHADKIIGKGHNQREGLKDPTAHAEMLAITSAATFLDSWQL
                     GDCTLYVTLEPCPMCAGAILNARISRVVFGAYDDAAGMCGSVENLCDQNLLNHKALVK
                     GGVKAELCRSLLNSFFNKIRSTEN"
BASE COUNT         8908 a         6750 c         5279 g         8847 t
ORIGIN      
        1 tattttggca agaattgctc atataagtat ctcgatgtca tttattgatt ttttttcaat
       61 atctttctgg gaagtctgat agcgaaaaat cacgaaataa gtcgcttcgg atttttctaa
      121 aggaatgctt gcaccatgcc aaacgccggg attaatcacg accgcttcac cgacattcac
      181 cctaaatatt ttcaaatttt cctcaggttc tccttcaatt aatagcggta aagcaaaagg
      241 tgcgtccacc ggtatcaaaa tttcaggagt atgtaaatga cactctaaac tatttacccg
      301 attacctcga ggaagagtga ccgtgcagag cccgatttcg gttttaccat tgattcgata
      361 attggcaata ttcgcccaga atttaaaggt atcaccctcg gcagttggtt tttctcgagg
      421 aagcgctacg acctggccga atttggcaaa attaacagga ttaacaggta taattttctt
      481 tgttagagta ttcataactt tatttttcta attcaaaaat ttttatctcg ccaccattaa
      541 gtacgatgtt atttagccgg gtttggtcgt cattccgctg aaattcatta aaggtggagg
      601 cactcccaag ttcattgtat tggacgaccc gcccttttaa tttggccgga atcgtaacgg
      661 aaatcgtttc cgtgcgtgta tcttgaacca gattggctgg ctttaaggga cgcttgctcg
      721 gtacgttagt agcaatccag gcaacgaata taatggtacc atcttccttt tcaaacgcac
      781 gataaaatac atccgaatca ggctgatgct cgacggaaat caggctggta acgttgcggt
      841 aaggattccc aaaaagcttg cggaaaaata ccagggcttt ctcagcaggt ttgggtctat
      901 aatcggcata cgcaatacca aggtaacgat tgttataatt atcaccgata acttcttcgc
      961 cagccggcaa atctttaatt tcataccagg cgattgccga taatttttcc tctgccaccg
     1021 ctaaagtaag acgtttaaat aaatctacgg cttgatactc cggtgtatgt tcataatcat
     1081 aataagcata ataggaatcg gaaacgtaac taccttgcct aaatgtgcta tagccaacct
     1141 ctgccatcca gagtgattga tcatctccat attttttaat tacttctgcc atcgcattaa
     1201 tattttcagt tattttttct acggggcgct cactccaggt ttcataataa ttgtgcatat
     1261 tgacaatatc tatataaggg ctgagaccat aatcacgaaa aagccgctcc agaaaatcgg
     1321 catgataagc cattccacct aaaactactt tggcttccgg atctgctcgt cggacagcct
     1381 gcgcccctat tttaataaat tcagcgaaag cggcgacgtc tcttgtatcc caataaatat
     1441 ccaaatccgg ttcattccag agttcccagg ttttaatgta ttttttatag cgagttacga
     1501 gatcatacat aaattcacca aattcttcga aatccagcgg ctggtcattc cagaaccaga
     1561 gtgtatcgcc tcttgaattc cagagcggag tatagcagat atagggaatt aaagttatac
     1621 cgtattgatt cacggcagtt ttaacataat catcccagaa taaccagtcg tattgatctt
     1681 tctgcgcctc aatagcatcc cagccaaaag aaatccttaa aagatcaacc ccagttcttt
     1741 ttaataattc catatcatta tagataattt ccatcgtcgt ggtctcttcg ggataatctt
     1801 cacacaaacc aactggaatg tgaacaaatt cagcgtcttt tgtcttcatt aagcgctgat
     1861 aatttggtga attgggtctg ctacaatcaa caataataag cataattagc gctaagaatg
     1921 attggagaca gtaatatttt ttcatacttt ttacctttct taagacgtta ggtttagtca
     1981 atttatacct tattaatacc ttaaaacatt ataataatcc atacttttta gtccattcat
     2041 ttattaaaaa tcaacccctg aaatcagaat aatgttggcg taaggtgaag tttcgcctgt
     2101 tcgaacaaag acagtgttgt tcgatttacg gtaaatattt ttaaattctt catgcgttac
     2161 gtactcgatt ggaattccct ctaataaacc tttaacctct ttcaaaatgc cattattaat
     2221 tgattcgatt tccttagcaa ggattgcctt ttccacaatc aattcctcaa gtactgcttt
     2281 taaagtatca atgaagcgag gtacattagg aaccaaagct aaatctacaa tttcggcctc
     2341 gcgtggtaag ggaagtcctg catcacaaat aactaattga tccgtgtgtc ccaatgatgc
     2401 gattaccatt gagagatgag agttcaagat tccgtgtttc ttcatcgatt ttatatcctt
     2461 tctattgatt taaataattt gaaatatcag cgagggttgg cattgaggtc tgagcacctt
     2521 gcttcgtcac cgaccaggcg gcaactttat tggcgaaagg tatcgcttca tttaccggtt
     2581 ttccagatgc cagcgccgcg gcaaatgccc cattaaaagc atcaccggcg gctgttgtat
     2641 caacagcttt caccggtgtc gcaggaaatt cttgccagct atcagaatta accaatatcg
     2701 caccgttttc gcctctcgtg gtaataacat tgccgactcc cgtattaatt aacttccgag
     2761 aggatgaaat aacacttgcg cgatccgtaa ctgactgacc agttaaaaat gatagttcaa
     2821 tctcattggg gatcagaaaa tcaataccat tcaatagact ggcaggcaac ttacaagccg
     2881 gggctggatt aagaataaac accgcaccat ttttcttggc taatatagcc gccgcttcaa
     2941 cagcactcag aggaatttcc agctgacata atacaatttt tgccctttca aatagctttt
     3001 cctttgatcg tatgtcgctt tccgtcaacc gcatattact tcccgaaacg acaactattt
     3061 cattttgtcc gtcggcatca acgtatatta atgccacacc ggttggcgtt tctgagtcta
     3121 taaatatctc ctccatatga acgccattgg attccatatt atttaccaaa cgatcgcgaa
     3181 aaatatcatc tcccatttta ccaatgaaaa tcgtatctgc tccaagtttg gcacaggcga
     3241 cagcctggtt tgccccctta ccacccggat aaattccaaa cttttttccg aaaacggtct
     3301 cgccaggttg aggatatcta tcaacttggg caaccagatc tatattggca ctacctacaa
     3361 taataaccga attttttgtc attaatatct ctttaatcta ttttaatttt aagtaattga
     3421 taatttgtaa ttactggcgg ctttaaaagt cctgatgatt taggaactgc ttccagagta
     3481 ttgattagcg tattggtaat agaaaatcgg aaggtatttt ttcccggtct gactaagtcc
     3541 gtaatatcaa tttgatatgg atgccaggga actattatcg gtccgctatc attaatctgt
     3601 aactctaaga catcttctcc acactcaata tcaagaaaca gcttaccttt acaaaagtct
     3661 gccggaatcg tcagctctgt tgagtaggtt ccagtgcccg agaaataggg gtatccctgt
     3721 tgacaccagt caccaatatt tacctccttt ttcggcaaaa caatactata cccatcgtca
     3781 ttcaatttca aagcaaaatc acccgtgatt tttataaggt ctaaaatacc gtcagttcta
     3841 cgttgtacaa ctaattgaac cgcgattaga ttacgacccg ggtggacata agaatggata
     3901 ttaatttccc gaatttcagc atctaattga ctgcgttggc cagtatcctt aacttccgtg
     3961 ccattaataa ataatcggaa agaacttcca ctaaaaccat ctataagaag ttttacacgg
     4021 ttaggtaatt cctcaatctg aaacggaatt cgataccaga gtgtctgcgg atagtggata
     4081 tcatccggtt cttcgggcaa ttgcatttcc caggcgccga tagtaactgg tagccagtga
     4141 tcatcatcat aatcgagtgc ggcgtagtta tcatttgaga attcatttga aaccagcatt
     4201 ttccatttag caagacataa tgtattatca ttttctattt taaaattcat aacctccggt
     4261 aatattagag gcggtggatt cttcggcacc aattggacaa tatccttttg tttaccacct
     4321 gtttttaaag tcaatttggc aagatctcca ttttttatat agccggttat aaaattggct
     4381 tcggtttttt ctaataccag gttggttgca ctaatataag aatcaggtaa gtcattatgt
     4441 aaaataacca gaaatgtttg gctggcgccg aaggttaagc gaaaacaaag tcggttattg
     4501 attatatcaa aaaccgagag ttcctttact gcaccggtcg ccggatccca tagttcaggg
     4561 cgtccaaccg tctccaatga aatgtccacc tcgccaatat cttgctgaag agtattaaca
     4621 aaatagaaca agtgacggcc atcttttacc ctgtgcaggt aaaaaacttc ggaatgtccg
     4681 atctggacat cggcggaaat acacgactca atggcttctt tgatatcctg tctacgccga
     4741 tcttttgcca gcccgcctcc ttgtattaag cacacccccg gctggagcat tttcagctgg
     4801 ttaacggcgt ttttcccatt ttggaaatct gccaaaatac aatttggctt gacgccaaat
     4861 agttcatcta cagcgcgaga ttttgacggt ttgccatcaa taaattgttc aaccggtaat
     4921 aaaatatcgg caattaactt accaccgtta tttacgaaag cttttagttt atccagagtt
     4981 gcttttttaa tatgagtcac cgggggtaaa atgatcagtt cgtaagattc atcggcgatc
     5041 tcaagtcggt taccatttaa ctgagcatta attagaatgt cctcatcaac ataatcaaaa
     5101 tcataatgaa gatgtaacag ggcatccgtc agataattaa aatccatttc aatcaatcgg
     5161 gaaatatcgt cgggagcctg cggtacatag tttgcccaaa tactcgtaat cgggtagaga
     5221 atgagaattt ttgcaatatg ttttccaccg gttagagcat gtcctaaccg cgccatatag
     5281 cgagtaaatt cattgtaata cttccaccag gtatggtgat aaaattgcga cggtggccag
     5341 tcacgcttgc gttcaccttc aatggaataa tggtagccat gattattaaa tagattaact
     5401 ccgagtgaat attcccagtt ggcaatccac ttcatccgtt ccaaagtgca atcccaataa
     5461 gtacctccca ttgattcgca aagtaatctc tttttcccta aatggtgagt aatcgagctt
     5521 gccatcttaa gttctacatg ttcgccgggc tgttcttccg aaccaatttt cggatataga
     5581 tgatccactc cggttaaatg taaattactc aaatgttgca tcacattgcc ttcacagcgg
     5641 gcatgacctc ttaaccaatt ttcaaataga atgtggcctg tgaataaaac gccgttttca
     5701 gcacaccaat ctcggagctg tttataataa ctatcgtcat actgatctga aagcgttttc
     5761 cagaaatcat aacgaattcg ggctgtatcg cgaccgacat tagcatataa agccgctagg
     5821 tgtggtttta tactatatcc attacgctca cgaaatatct tgaacatccg ctggctccag
     5881 ggaataacat aattatcagt ccctacttga aaatagtgca tagccggttc atcggtataa
     5941 aatcccggaa cgactttccc aaattctgcc cccactgcgg tcttatattg ttcatgcgtt
     6001 aattcaataa aacgtttagt ggcttccgga tttaacgcat ctatatagta atctatttcc
     6061 ttttctaaaa aatacattaa tacccagttg ccagctggcg cttcccacgg aataacttta
     6121 ttaaatgata aattcggagt tacatcaata atatttttga tgccggttcg atattcctca
     6181 gctgaacaga tataagcggc aatcggtttt gaatcgccgg tatttatata gcgtgaatcg
     6241 gtggcttcaa gatagatgaa aagtggacca ttaataaata gtgtaaccat ttccaggtac
     6301 ttttggctta aatatggata ttttttaata acctggcgtt cggctgtgcc gcttggccag
     6361 ttcatctcat cgtaaatcca gatgttcaag ccgatttctc gacctttttt aacggcaaat
     6421 tttacccgct caaaccattt ttcggataaa tagggaattt ttaacccgaa gcgtccgtga
     6481 ataaataaac ccgtaatacc tttggctttg tattctttta actgccactc catctccgcc
     6541 tcgtccattt cgccgttcca aaaccagaat ggcaaaatac ccatttccag cgggggattc
     6601 ttaaaatttt cacagtttaa ttctttgtct ggccacagtt catttagcaa cttcatcaag
     6661 taattcctta acctttgtta attttcaatt tgcaattatt agtttttcaa tttgccaaaa
     6721 tatcaatcag ttattttaac aattggaaaa tcttagtcgc cctcgggatt ggctattttt
     6781 tttatcatag acaagtaaaa tgcggtaaaa aagggataat tcatcttcat taaaaccaat
     6841 tcatcataac ctaaattgag attattaact cccatgccaa tatttttaaa accttaacat
     6901 cactgtaaac tgatgggctg aattaaggta atcataatcc acgaaggaat aatccacgac
     6961 aagattggta aaatccatcg gaatatttaa tgccgctcca acactaaaat gcccctcttc
     7021 acgattggta cggtaaccag tacgcaaagc aatccgattc catagccagt actcggcgcc
     7081 aatatttaac cgttcggcat aatcattaga gtgaatcgcg tcaatgttca gagaaagacg
     7141 ctgtttatta ggatttccgc caagaaacaa atctttgcca cctaataaat ctattgacgt
     7201 ccctatccga aatactaagg ggacctcata ctgttgttct tctctctgct gggttttcaa
     7261 ccaacgggct tgcgaagcaa aattttgcgc cgtcattgca agccgaatgc ctttccaccc
     7321 agtattataa tacgtaccaa catcaaaagc ccataaactg gttgaatagg tataaaggtc
     7381 ttctcttaaa tatttggtgc gaatacctat agacaatcta tcggtaaact tttttgaaaa
     7441 tgaaagacca actgagagat cgcccccgga gaaatttaat ccggtgcgag tatcaagttt
     7501 accatcggga ctggtaacca gggcttccgg aatatcaccg taatccatcg cgatataatc
     7561 aagcgcaata cttcccaatc ggtcaatcgt tagccccact gctccggcgg taacatttag
     7621 atccccaatc cactttgtat agctgaaaga agattctaat cgttgcatct gagtaatggc
     7681 ggcgggattc cagaaaacag catcaatgct atttaaattt gcgatacagg cttcccccat
     7741 tgcagatgag cgggctccta tcccgatttt taaaaactga agggaggtgc gaccggcttt
     7801 attaaaacct tctaccctca gatttgtagc gaaaaaaatc attgcagaaa gagttacaat
     7861 tttaattttt ctattatgta acatcatttt ttattcctcg gcatatcaat tttactcttt
     7921 agcttcattc agcgaataat cattagctta ccaatcgttc gtttcccttt tgtcccttcg
     7981 gcgtgcgatt caatggcata aatataaaga cccggtgcta ctctcaaacc agaatcggta
     8041 cgttgatccc aggctacact gccaaataaa ttaccgtaag tatctggccc atgttcaatc
     8101 gttttgacta aatcaccaga aacggtgaaa attttaatcg tcgctctttc cggcagatta
     8161 cgaaattcaa ttttataagg cgtcggatca ccaaaactac gctcagggtc accttctatg
     8221 tagggattcg gggcaacgaa tacatcccag ccagcagtaa tgtcaactgg tcctggtctg
     8281 gctcccgtca aaggagtcat attgtaatca tcactgacca gttcccctag attatcaacg
     8341 atagtatgat tggccgaaac ccaggtttta ggatttgaat aataagcctg aacataatac
     8401 cggaaatcaa aaccaaactg caaatcctta tcgagataca catatttacc aatttctgca
     8461 ttccaatagc gattggcttc ttcaatggga atgtcaacaa cttcatccca aatgccccgt
     8521 ttgtcaatac cgcggaatat tcgatagcca ctcagatcag ttgaaccatc ataaaatgga
     8581 ttcccagccc cgtccgttat taccgtttct tcggctagtt tatcccattg aatcgcaata
     8641 atggcagtat ctgaaccttg agaagcatta gttgcgctac aattaggggc tggtggtgat
     8701 tcgggcacat ccgccgctaa attaattcct tcaaccgttg ctccaaatcc ccactttaat
     8761 gctctacgag cggcttcggc attacggcga atatcggcaa tcgaactatc gggataatag
     8821 tgattgggat ccccacgaat cacttcatga taatccaaac tcccggctac ttcagcgagt
     8881 attaaccgca cagaatcacc tggcgcgaga taatatgggc caaaagtagc agtataaaaa
     8941 caatacatat caccattatt ttcttcagga atccgattat gagtgataaa atcgaacatt
     9001 cctttttctc ccagccgttc aatcgaaaag tactcattca tatccaaagc tcggccttgg
     9061 aattgctcac cttcatcgga ataaatattg aaataattag gatccgcctc aaaaggatcc
     9121 gcgtcagaat ccgtcggagg agttctatac agaaatacaa atccaaagca ggcgggatct
     9181 tgcaattcta tattttcata actttgatta tacttcaatc gtgtttgcca atccgatctt
     9241 ttactatagg tatcgtttgg atgcggctct cgccccccat taaaatcccg actgtaaaaa
     9301 aacagtccct tgctatcatc cccatcattc tcgaccgcaa aataatcggt atatgttcca
     9361 ccattttcaa gttgtttcca gcctccgaat ccaccagccg gagccgccga actcataaaa
     9421 tcaggatcgt aatgaaaatt cagatgacct ttagttgaga cctggattcc gctatgaaaa
     9481 accacaaata ccgaatcaaa agtttgttcg taatgtttga cttctcccag agctggaata
     9541 accatctcac cagtattgat aaatacataa tcataaacaa taaaatcatc ataatccgga
     9601 aaactccaag ccatagagcg catcttaacc gaaatgccgg ttgctgttgg gaattcgcag
     9661 taattgattt cctccggcaa taatggatta aactgggacg tccctacgaa attattcgtt
     9721 tttataaagg gtggataatt ccataaaata ccatgacttc caccagcaaa ggagctgtga
     9781 taaaaaagat attcctgata ttgtggatca taatccggag ctactaatac atcaccgcct
     9841 ttaattacta atgccggacc actacgaaaa ttgtgaaaat tggcatcagt aacccagccg
     9901 ttagcaagtt gttcattatt gggaaatgaa tatccagaaa agccaggata aaatcccaaa
     9961 ccggaagtct cttcataatc ccagacgccc aaatctccga ttaaacctac attattgagc
    10021 gtttcccata gtttcccgcg agtatgggtt gacttccgct gagcaaaaga ttcactccaa
    10081 ccagtcaaca gtcctaaaat gataactaag tgaatataat aattttttac tttcatagta
    10141 ccaaccactt ctattattga aaacttaaag ccatgcccac ggtgattttc ctgggaaggt
    10201 ttgtatattt ggcataatct ggaaaagggg caccgttggt cattttttcc ggatagacca
    10261 ttaagttaga attgatataa cgaacttgat cttcggccga aatatcacct tccataagat
    10321 caacattatg ccatttatca ttaaaagcat tctgcatctc aatgaaaatt gcaggcctgg
    10381 tacccttgaa tcctttgaat tcttttctca cccgaatatt ggcattctta tttgccgggc
    10441 gaatgatgtt aaccgctccc tgataaacgt cacttgaaat ttgcttatgg ggatacgaaa
    10501 cataataatc aattgagaac tgtgtatttg ctaaaacacc acctaacggt gaaaaattcg
    10561 acggagccgc cagagtcagg ttagcactga gactgggacg catatagtat agttgatcac
    10621 caatatcaaa atcctggctg gcatgaaccg ttacattggg agggtaaata gtaaaagggt
    10681 caccggattg tcccacgata cctgtttccc aggtataagt caaataacct ccccagaaac
    10741 cacgtaaagg acgtcgtaaa gtaaactcga cgcctttggc atcagcataa tcagaattac
    10801 cggatgtatg atactgttgt tcattatatg ttatgacagt aatactccga acatcgttag
    10861 tgcgatcctt atagaaagcg gtaacatccg ccaatactgg tccaaaatcc tgctcgatac
    10921 ctaattcata cgccactgtt ttccgcgggc gcagttcacg attccccaga ttacggggtt
    10981 cgatactgcc aaattcagga tcaacaatat aagttgataa aatgctatag gtctgctcac
    11041 cttcaccata ctcgccgaat gatgcacgtt ggaaaaaatg accataatta aaatgtaaga
    11101 gggatttctc ggttattgga tgtgacaccc caattctcgg ggataattgc ccaaatgtat
    11161 aggttggttt ggttttggga ttcggttcac gtccttccat ttctgccgta ataatatcga
    11221 aaggatcgta tggatcgagg taaataacat cattaggatt ataaaaatca tatcgcagtc
    11281 cgagattcat aattaatcct tcaaattcca gcttatttga ccaccagatg tttccctcat
    11341 aaggatggta gattttatcc tcaacggctt cccaggcgcg cgcctcttga tatcggtcaa
    11401 aagtgtaata agtaaaacca aaaccactcc gcatttgcca gtttttattg atctgacgag
    11461 tcaatttacc cgtaagcgtc aaatcggtat tacgatcctc cttacgatac cagtaaccga
    11521 tatagtgtaa accatagatt tccgcccagg catcgggtat cgcaaagcct tgcgaatcgt
    11581 aagctcgtaa ataatcccga cgtatatcgc tggtggtcca gtctgccgta tcgctcggaa
    11641 acttcgtctg gctctcataa ttgaataccc gcgataaatg gagttcgtaa aaagtatttt
    11701 tatcgagatt gtgaacaaat ttcaaagtct gaccattgcg cctatataca gggtaaccag
    11761 cttcatcata ataagcatac ttattaccat aatcaccttc gtattccatt gattccatac
    11821 cgtgacaagt attatcctga ttataatatc ccgaaaatac caataattta tttcctccca
    11881 aagaatagct gattttataa gtaccatcaa gccaactacc catcttgcga taagcattac
    11941 ccgtaatcgg tggtgccgta cgataacgac cggataaaaa gaacctaagg tttttaatcg
    12001 gagtcggtcc accgaaagaa aattcggttt ggtaaccact tcgattggta taatccgtca
    12061 agggttgatg agtatctata taaaattgat aagcctgctc cggtgtccag gaacatgacg
    12121 gatcgttgcc gtagcgacca ttcggatcaa cccacatatc gggattatca atccaccatt
    12181 gcaaatggcg tgcatgcgta ttctcccaat atcgctctgt cgaataatcg taaagtgcag
    12241 ttccccagtg atataaaccg ggaggaatcc agcgaaattc tactgcaccg acataatgat
    12301 ccggtccctc tttagtaatt atattaaaaa cacccgattg agcttcaccg tactcagcat
    12361 caaaaccacc ggtaataatt tccagttcct caattgccat cagattaaca ttaaaataag
    12421 gctgagaatt caaaggattt cgtgcccgca ttccatcaat cataaatgcc acctgatcca
    12481 acgttcccat tcggactgtc aactcaccgt agggattata atcaattcca ggcattgagg
    12541 taagtatatc ggaaagctga gataccggcc gggattttat atcagtttct tcaacaacaa
    12601 cacgactcga agttttatct aactcgatca gtggtctttc ggctacaact gtcacggctt
    12661 cgccaaattc caatacggtc tcttctaact caaaatctac gcgagtggtg tggttaacac
    12721 taacgtttac ctcagtttta ataacttgtt tatatccaat catcgaagcc tggagactat
    12781 aaataccggg tgcaacattt atgatataat aatcaccttc catatcggta gcggctccca
    12841 tttctgtccc gatgataata atgttcacgc caggtaaggg attgcctgtc ttcttatcaa
    12901 taaccttccc tacaatctta ccatcactcg ccgcatatcc caacgaaata agcaacataa
    12961 agccgaggaa agttcgacta attaatctcc tgatttgcaa atatgctttc ttggaaatca
    13021 ttattttatc tctacgaaag tttgattgtg tcgttttatt tattttacta atcattattt
    13081 tcaatcccat taaggttcaa tgacagcttt aataaatcct ttgggctttt cctgcgcggc
    13141 gcaaaacgcc gcctcaatct catctaattt aaatttatga gttatcaatg gtttcatatt
    13201 tattttctgc tcatttaaga gcttgatccc aatttccgct ccgcgtaaaa ttgtcgccat
    13261 atctctaaaa tgagcattga caatattaaa agccatccaa ttccaatatc ccaaatcttt
    13321 aataattctc tgccctggat gatatccaaa aataactaac ttcccttcca tccgtagtaa
    13381 gtcaacggct aagtcgagac ttgcttgatt gccaccgact tcaaatacta catctgctcc
    13441 ccgaccatta gtcatatccc aaataatagc cgggacatct tctttctgcg gattaatgac
    13501 caaatctgct cctaacattt ttgccttatc gagaatttca gcgcgaacat caacgccaat
    13561 aatccgagaa gcacctttca aagcaatctg ttgcatcaag atgagtccca taaatccagt
    13621 tccgactaaa actaccgtat cgcctaattc aactccggct ttgattaccc catttgtcgt
    13681 gcaagctatc ggctcagcca tagcttcggc aaaactaatc tgatcagcaa tgggaaaaat
    13741 ttgattggct cgaactgcaa cttcttcggc gtaaccagcc cgatcagtcc agactgcaac
    13801 tcgatctcct cttttaaatc ctttaacggc acttccacat ttcagaattt caccagacac
    13861 ttcatgaccg atatatcgcg gatattccaa gttatcgaga gtttttgccc attgatggat
    13921 ttcggaatga caaacgccac aggctcggac gcgaactaag cattcatcat cttttatatc
    13981 cggacttgct tttgtttcta ttctgaaatc atgagcacca taaagagccg cgatgcgcat
    14041 attattcctt tctttacatc aatttttcaa cgaccaaagt atagtcagga ggggtggata
    14101 aaaatttacc acccctcctt tgactttttt tatttcataa gtatcatttt acgggtagaa
    14161 accactttat ctgtaactaa ctggtagaaa taaataccac tagataaact agatccatcc
    14221 cagattacgg aatagacacc cggagaatat gtccggttat ctattaaagt cgcaacctct
    14281 ttccctaata cattataaac cttcaacgta atcggaacac gttcattgac ttcgaaccgg
    14341 atcgtagtgg tcggattgaa tggattcggg tagttctgcc gcaatgcaaa cctaactggc
    14401 ttctcgttaa cttccgccac atctattccg tttaatccag taccgaaatt cagcagagcc
    14461 cagtattcag aattttgctg aatataagtg gcacaccaac ccagatctcc cggatctgca
    14521 aacggttcat tcggcacatt cggctgccag gtgtaatatc catacgaatc acccgtcgtc
    14581 tcacgagaat acgtcgaaga aaaactacca ccaacattaa atccaaccga agcacttgcc
    14641 ttaacattgg gattgtaaat cgctaactct acatcccata ctccggtctc cttattccac
    14701 tgcgccgccg atcgtataaa cgtcgaagga tcaaatatta ccgccgagtc taattcaggc
    14761 acatataccg gataaccatc attaaatgtt acccgatctc ccattatcat caaatgataa
    14821 ggaccatccg gtgccgccat cggattccct tcccagttat cccaagattc cactcctaaa
    14881 cggttcgaaa cccctataaa taactggtca ctcgcccaat gtccagatgt gtcagtcgga
    14941 tccaaaaatt gaccacccca gtacaaatcc gtcgtgtcat tgacaaactc atcaatatgc
    15001 ataaatacat atagagtatc atccgtccac aacaaacgcc catacaactc atcatactcc
    15061 ggctcggcta tctcacgacc ataagaactc gcccagatat tgtaacccgt ctcagtcact
    15121 aaattcactt ccgccgcgtt accccaaacc gactcatcca ttactccatc aatcactaca
    15181 tccgcaggtg ataccttcgg aacattcaac tctaaacgca caattacgtc gtccggtccc
    15241 ggcagaaaat tcagcagagc ccagtattca gaattttgct gaatataagt ggcacaccaa
    15301 cccagatctc ccggatctgc aaacggttca ttcggcacat tcggctgcca ggtgtaatat
    15361 ccatacgaat cacccgtcgt ctcacgagaa tacgtcgaag aaaaactacc accaacatta
    15421 aatccaaccg aagcacttgc cttaacattg ggattgtaaa tcgctaactc tacatcccat
    15481 actccggtct ccttattcca ctgcgccgcc gatcgtataa acgtcgaagg atcaaatatt
    15541 accgccgagt ctaattcagg cacatatacc ggataaccat cattaaatgt tacccgatct
    15601 cccattatca tcaaatgata aggaccatcc ggtgccgcca tcggattccc ttcccagtta
    15661 tcccaagatt ccactcctaa acggttcgaa acccctataa ataactggtc actcgcccaa
    15721 tgtccagatg tgtcagtcgg atccaaaaat tgaccacccc agtacaaatc cgtcgtgtca
    15781 ttgacaaact catcaatatg cataaataca tatagagtat catccgtcca caacaaacgc
    15841 ccatacaact catcatactc cggctcggct atctcacgac cataagaact cgcccagata
    15901 ttgtaacccg tctcagtcac taaattcact tccgccgcgt taccccaaac cgactcatcc
    15961 attactccat caatcactac atccgcaggt gataccttcg gaacattcaa ctctaaacgc
    16021 acaatctctt cttggcttaa tgccaccgat tgtaccgcca ctaatagcaa taatgctaac
    16081 aaacacatat taaccttacg catacttacc tccatttggt taataaattg gttaataatt
    16141 ccttgtccta ttttacaaaa ataaagtaag cctgacttat aaattacacc tcctttcatt
    16201 tgttttttta cctttctcat aattgatact caacgtttat tgatggaata attgttttag
    16261 tatcatattt accataaaaa tattcatcct ggcttttctt gatatcttta gcaactccca
    16321 cagaataagc aatactgaga tctagataaa attggttaaa taaaaatcca ataccagccg
    16381 atattctatg ttgatttaca ttcggcaata aaagactata actacgatct gaatttgggg
    16441 ttgtctgata attatatcca acccggtact tcaaattatt ctcgcctgaa tattccagtc
    16501 cgatagcaaa tacaaaacta ttacgatcgt aaaacggcat tcctgaacgc cagtaaccgg
    16561 tgatactatc acgcggagcc gcagctatta tggcagacca ggcagtgtca ctataattaa
    16621 aatccagttt ggattgagtg cttccccaga attcgctgga tagatcaata tttagattta
    16681 gttgatcggt gggtttgtat aaaattccta taccaccagt catcggcatt tgaaaacgac
    16741 tgtgaagtcg cgatttcttc gccagactat caagcgcata aaagcaatcc gttttagcat
    16801 taccttcaag gtcaaaatct acgccgctcc gaatgctccc gcctacctta atcgtcgttg
    16861 ttataggtaa aataaatcct aggctccaac ctatggcatt atcctcttgt tctaaattca
    16921 aagcgtagac cggattagtc ctgttcgtct gccactcagg attcgaaatc ggaaactcaa
    16981 attgttggta aatctgataa atattaatcg cggcacccaa tgacattttc ccgacttgta
    17041 atccgaaaac ctgcgaatag gcatccgcaa tcaggcgtga ttttaattga aagccataca
    17101 aacgggttat tccattttca gtaaacatta atgccagtgg ccatcgaacc gaataatcaa
    17161 gggctcgatt atagaccata gccagcccaa acttttggtt aggcacccag taaatacccg
    17221 ctgaaccgct gaaatcattg aataaatagg aacgatggat atcacgactt cctttttgac
    17281 attgttgttg cccggctcga tcaatgatat tcatcgccag accaaaaccc ggtgtgctta
    17341 aaattttacc cgggttttga aatgtagttg ttataccatc taatccggca taattataac
    17401 cccccaaagc tagggatttt gcactggtta ttggagcagt actcgcatct ccggcttttg
    17461 taaaatccga aagaatcaaa accattgaaa ttgtccaaaa gataattttt tttctcactt
    17521 agaatttcct cttcaataaa actctttcaa atcaccaagt attaaaggtt atatatgaat
    17581 gatacttagt ggatcttaag tgatcaaccc cattccaatc cttaccagcc agccagaata
    17641 aataggtctt atttcgggac aaaccttttg caggatattc aacaaatacc ttcgtcccgg
    17701 gaatcgggct tcctattacc agcggctgac ctattacatt gttttttcca tatacctttt
    17761 gaccacctac ggtatccacc gaccaggctt cccagactat ttttttgggg ctatagctct
    17821 ctcctaaaca gattccgacc gccgctacga gagaatcttc aacacttgaa tcaataatct
    17881 cccaggttat gaccggtgaa ttgctggtat ccgtttcaat aagagtgatt ttggcaaatc
    17941 cacctctcaa cttaatgctt gccatatcaa catttgtata aacatctatc cagcaactct
    18001 tttgaacatg actgtaactg tctatccata aagtatcctc attggcaatc gtcgttaccg
    18061 tgtccagttc tgccattaag attactttac cggcattttc tactacggca tcccaagcgg
    18121 cttccttaat aacccaaaat gtataggtgc tgtcttcaat taatgcttca aaatgtccac
    18181 catattcatt tgttatatca gttaccccct cgggcaattc gccatattta accgggtagt
    18241 gcaaattgtc accatcaacc tttattaacc attgcaaagt actgtcaagc cgtgcttgat
    18301 cgccgcgatt tactccaaaa gcacatccat atccacccaa ccaagtaaat ttgggcgtaa
    18361 atgtttcctg ttcaataata atattgttca ttggccgcgc gccttgatac ggagccagcg
    18421 tattcttctc cccacagtac cttaaaaggc aaagtaaaaa tagaattaaa atgctacttg
    18481 ccgaaataaa gtggtttggt ttaaataagt aattcataga ataaacctta tgctgttttt
    18541 actaataatt attattagcc tcttcttaat cacgatacta tcctttaatt ccacttaaag
    18601 tgataccttt tatgaaatat ttttgagcga agaaaaagac gattaaaatt ggtaaagttg
    18661 ccattaccga accagccatt aataaatccc agtctgtaat ataccggtcg cggaatatag
    18721 ctagtcctaa ctgaaccgtg cgtaaatgat ctgaattgac cacgattaaa gcccagataa
    18781 aatcattaaa agtccccata aatgtaaaaa tggctaatgt tgccagagcc ggtttcgata
    18841 aaggaataat tatctgccac aatactctaa attttccaca gccatcaata taagccgcct
    18901 cttctaattc cttaggaatc gtcaagaaaa attgtctcaa taaaaatgtg ccaaacgccg
    18961 aagccaagcc cggaacaatc atagcttgat agctgtcaac ccatccgagc cagtataaaa
    19021 ccataaaact aggaatcatc gttacctggt aaggaatcat catagttcct aaaaaaatat
    19081 agaaaagaac atcccgtcct ttgaattgta accgagcaaa agcataagcc gccatcgaac
    19141 aagtaattag ctgccctaac gtcacagtac aagctactat aagactattt aaataatatc
    19201 gcccaaaggg agccgcttga aaagcccggg cataattctc aaaatggaac tttgaaggta
    19261 gccactttgg tggatacaca aatatatccc caatatcttt taccgaagtt aggagcatcc
    19321 agataaaagg tgcaattgtc gctaaagcta aagcgtatat aataacatga aggataatat
    19381 cttttactat atgactactt ttccgaaaat ctatatccat tataccgcct cataaccggc
    19441 acgggtttgc ataaacttta cttgtaatac cgttagcacc gctaatattg caaagaaaac
    19501 ataagccgcc gcagaggcat accccatctc gaaaaatttg aaggcattct cataaatata
    19561 aaataccatc accttcgttg cgcccagagg tccccctgaa gtcaaaacat agacaatatc
    19621 aaagacctga aacgagctga tcattgacat tatcataatg aaaaatgtgg taggacttaa
    19681 catcggcatt gtaatattcc aaaatttcga ccaccatccg gcgccatcaa tttcggcggc
    19741 ttcatagtaa tgatcgggaa tcccttgtaa tccggcggaa aataaaacca tatttgtccc
    19801 aaatgttttc cacactccca tgataataag agcaaccata gcggctttcg gttcgttgag
    19861 ccaatttacc gagggaagcc ccacgagatc cattaaatag tttaacaaac caaaattggg
    19921 gtcataaatc caacgccaga ttaacgcagc cgctaccggg gatataacca caggagtgaa
    19981 aatcgccgta cgtaaaaacc gttttcctgc gatgcgtcgg ttaagtataa tagcagttaa
    20041 cagagcaatt accatattta aaggaaccgt accaagtgtg taaataaaag tatttttcag
    20101 aaccatccag aattcaggat cagaaaataa ccgacgatag ttctctaaac caaggaattc
    20161 ccgcccacta ctgaacatat tccatttatg aaaactgaga taaaacgaga agaataccgg
    20221 gaataaaaca aaagcgcaga aaataaccaa cgtcggagat aaaaatccga gcgctgtgag
    20281 aatattcttt ttactattcc catcccggcg atttataatc gtctttttaa ctctggtaga
    20341 tttatttagc attctgccaa tacgattgta agaccttatt agatttcgcg gccgctttat
    20401 ccagagctgc ctttggatca ccgccaccaa gtgttgcttc ttcaatggct tccgccatat
    20461 gtcgagtaat ttgtaaagcc ccataatcca tagagcgctg aatctgggca acttccatct
    20521 ggtctacaaa tactttaaaa ttcgggtttt ctattagata tttcttgaac tccggtattt
    20581 ctgatactga ccgccgcacc ggcaaataac cggatttcat agcccagcga gcttgaatat
    20641 caggccgaat gatccacttc acaaatgacc aggcttcatc gggatgttca gattgtttga
    20701 aaataaccag atattcgcca ccgcagatgg tcgcctgttt tacaggacct ttgggcaacg
    20761 gtgcaaacga ccaattcaaa tttttcagca ttcgattaaa tcgcgggagg ttccaaggtc
    20821 catccatcgc catagccacc aacttcgagg caaaagatac atcgtaatcc gtagtaaatg
    20881 tccgtaaatt caatttgtca tatatgtttt tccagagtgt cagagcctta actcccgctt
    20941 cactattata taatacttta gattggtctt cagctataat ataaccaccc gcttgccata
    21001 aatagggaat ccactgccaa accatccagc cactgagagg acccgcggct ggaaatatgg
    21061 gcagaaaaaa tccgatccgg tcaaattttc cgtcattatt aaaatcttta gataatcgca
    21121 cggcatattc ttctaactct tcccaattcg atggagcata accatcttga taaccggctt
    21181 ctttaaccat atcaacatta tatatcaacg ccaggttagt ggcttccatt ggaatacaat
    21241 atatcttccc ccgcagagaa gaatattgta ataaagcagg atagatatct tctatttcgg
    21301 atttactaag tccattgttg cttttgataa aatgatccat ctcataaatc gcatccgcgt
    21361 caactaaatc agccagaaaa tcagagtgaa tccaggaaat atcaggcgcg gtcttactct
    21421 gaattgccgt gataagtttt tgaattagag catcaccagt tggcacatat tgagctttta
    21481 ttttgacatt gggattttca gtttcatatt gttcgattaa atcttcgagc gctggtatag
    21541 tagatgcgac aaaactatgc cagaaagtaa tggtgatttt atcatcttta gtagcctctt
    21601 ttttcccaca tccaataaat agtaataagc aaaacgatag taaaaaatat ctactccatc
    21661 ccctcagatt ggctgtaaaa acgtttgcgc atttctgggg tgagtgttga ttgccgatat
    21721 ttttgaattg catagttcgg acctaatttt tattggtttt aatcgggatt aataacagaa
    21781 tttagatttc ggacggattt tcgaatattg aggacaggtt ttaattcgat cctggttttc
    21841 tcttttcttc ccttactttt taaatcgtca tacaacaatt taaccgccat ctcacccatg
    21901 atctgccgag gttgctgaat agctgtcagc ggggtaaaaa gatacggggc aaaatctaca
    21961 tcatcaaaag aaatcagcga aacatcttga gggacattca atttttcttc gataattgca
    22021 tctaaggtac ccaaagtaat taaatcgctt gtactgaata tcgccgtagg tggattggac
    22081 attttcaata gtagtttggt ctcaatatat ccattttctc ttcgataatc gttaccaacg
    22141 attaaattct catcgatttc gatattatat ttaataaggg catttttata acctttaacc
    22201 cgcatattgt tagtagaagt attgggaagc ccttgtataa ttgcgatcct tttatgacca
    22261 tttttgatta aatattcaac agctttatag gaaccagtat agttatctac aaccacagaa
    22321 ttggtatcaa tggaatcaaa acacctatcc agtaaaacca aaggaatttt atcatcaatg
    22381 agttcctgga tatgatctga ttttaacccc accggcatta taataaaacc atctactccc
    22441 ttgctacgca gtaattgtat ttgttcaatt tcagtatctt gattttcatc agtattgcac
    22501 acaatcaaag tataaccaag tttataagat tcaatttgaa taatgcgcga aacatgagca
    22561 aagaaagggt ttgaaatatc cggaatcacc agtccaatgg catgtgtctt tcgcagtctt
    22621 aaaccgcggg ctaattgatt cggaacataa ttgagttctt gagcaatttt gtgaatttta
    22681 tcggcagttt catctttaat ccggtttact ttccctttat tattcaatac ccgagaaact
    22741 gttgaaacgg aatatcctga ttttttggca acatcttcga gggtcgctgt cattttgaaa
    22801 ccactttgta aatctgcgca atcattttcg caataagtat agtaaagaaa aaaatatttg
    22861 tcaagctaat ttttcagagt attacgaaat ttttctacgt tcatctaata aatcattgat
    22921 atcattcaac atatagtatt ataaatttat tgttacatac ctttaaattg cgaaaatctg
    22981 gggatgattt tctacctgcc gctaataagt cgagtcagat taatattgtt tttcttttaa
    23041 ccgctcagaa cgctaagttt ttatcaatca taaatccgcc agccaaactt atactcgtca
    23101 gggcaatgag tgttaaccac aaaaaatact aaatgacaca tagaactgca taaatgcttt
    23161 tttgcataat tgattgtggt aaatggttac tgttagctgg ttttggttan nnnnnnnnnn
    23221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atatatcccg cgccttttcg cgtggttcgt
    23341 gggcaataac taacgtgcca ttttatttta ggacgatgct gacgatttta ttctttacca
    23401 caaccgtctt aacaattgta tgcccttcaa ggtaacgctg gacattgtca atctgtagag
    23461 ctttatcaat gcaagcctgg tcttcggaat cgcgttcaat atcgaaagtg ccgcgaattt
    23521 taccattaac ctgcaccgcc attgtaacga gatcatcaat aattaaactc gggtcggaat
    23581 ttggccagtc aagatatgcc agactttggt cattacccat tagattccag atatgttcgc
    23641 taatatgagg cgcaaatgga ttcaataaat gcagaaaagt cttcattacc gatagcggtt
    23701 taaccgccaa tttggcgatt tcattgacca agatcatcat ctgggagatg gcggtattaa
    23761 agcgcagatt ctcaatgtca tccgtaatct tgaatatggt tttatgtaaa atagaattag
    23821 tcgaagcatc gggcgtaata tctttaatat ccgggctgat taaaccttct tccgttacgt
    23881 aaagattcca gacgcgattt aaaaatcgaa aaactccatc aatgctggaa gtattccagg
    23941 gcttaacgcg tgtcagcggt cccataaaca tttcatacat gcgaaaagta tccgcgccat
    24001 aagtatctat gatttcatcc ggattaatga cattgccgcg ggacttgctc attttctcgc
    24061 catcttcacc cagaatcata ccctgattga ctaatttcat aaaaggttca ggcgtcgaga
    24121 catatcccaa atcatataat actttatgcc agaaacgagc gtatagcaaa tgcaaaacgg
    24181 cgtgctcggc accaccgata tataaatcaa ccggcatcca taacttttct ttttccctgt
    24241 cgcaaaatgc ttcatcattg tgaggatcga taaaacgcag atagtaccag cacgatcccg
    24301 cccattgcgg cattgtgtta gtatcgtagc ggcaatgttt cccggtttct ggatcgataa
    24361 aatcaaccca ttccggcaaa tttgccagcg gagattcgcc tgttccgctt ggttcatact
    24421 tttccagagg cggcaattcc agcggcaatt tcgattcatc aatcaggtga atattgttat
    24481 cctcatcata atacaaggga aacggctcac cccagtaacg ttgacgggaa aatatccaat
    24541 cacgcaattt atattgaact cgcgattgtc caattccctt ttcacttaac cactcaataa
    24601 ttcggcgttt agcctcagcc acatttagtc cattcagaaa tcccgaatta actaatatgc
    24661 cgtcgccgac ataagcttct ttggtaatat cgcccccgga tatgacctcg acaatttcaa
    24721 gtccgaattt cttagcaaat tcccagtcac gttgatcgtg tccgggaacc gccattatcg
    24781 ctccggttcc atacgacatt aacacataat cggaagtcca gatcggaatt cggcgattat
    24841 tggctggatt aattgcatat aaaccggtaa atacgccggt tttctccttc gctaattcgg
    24901 tgcggtccag gtcacttttg gaagcggccg cttgttgata agcttctact gttttcttgt
    24961 attccggtgg aactaaagtc ttcaagatcg gatgttcggg cgccagaacc atataagtag
    25021 ccccaaataa agtatccggt cgagtagtga agactttgat agtctgatcc gaaccttcga
    25081 ttttaaaatc tatatcggcg ccttcgctcc gcccgatcca gttccgttgc atttccttaa
    25141 tactttcggg gaaatcaacc agatccaggt cttccagaag gcgatcagca taagcggtga
    25201 ttttcaacat ccactgccgc atcggaatcc gaatgacggg atacccaccg cgctcggatt
    25261 tgccgtttat gacttcttca ttagccagaa cggttttcag ttccgggcac cagttgaccg
    25321 gcacttccgc caggtaagcc aaacccattt tgtagatttg ctgaaaaatc cactgagtcc
    25381 atttgtagta ttttgggtcg gttgtattaa tttcgcgatc ccagtcatag gaaaatccaa
    25441 atgatttaat ttgtcgtcga aaggtattga tatttctttc cgtagttatt ttcggatgag
    25501 ttccggtttg gagcgcataa ttctcagccg gtaatccgaa agcgtcccat cccatcggat
    25561 gcaagacgtt gaaacctttt ttccttttat aacgagcgat tatatcggaa gcgatataac
    25621 cttccggatg cccgacgtgc aggccggctc ccgatggata agggaacata tccagcacgt
    25681 agtattttgg tttattagat ttatcagggg ttttaaaagt cttgttatct tcccaatatc
    25741 tctgccattt cagttcaatt tctttgaaag gatacattaa ttactcgttc taaatttgga
    25801 aatatcagta taaaactctg aacttgattg tatttggtat attcattatt tcaaggagca
    25861 cttcattatc gtgatttttt tcaatatcgg ttatcacgta accgatatcc tcggtagttt
    25921 ttaaatattg tccaacgata ttaataccat gcctggctaa aatgtgattg atttgggaca
    25981 atatgccagg gacattattg tggacgtgta taaggcggtg ggcgccattg aattggggca
    26041 attgcaaatt gggaaaattc acactcataa tcgtattgcc cgagttgata aagctaataa
    26101 ttttcgaggg tacgaattga gcaatatttt cctgggcttc ctcggtattt ccgccaatat
    26161 gcggtgtaat gattgtattc ggcaaatggc acaattgaca ataaaatctt tcctgattat
    26221 ttaacggttc agttggaaaa acatcaatag ccgctcctct gacctgacca ctttgaaggt
    26281 tttcagctaa agcttcaata tccacaacgt gaccacgact gaggttgaga aataaaacgc
    26341 ccggcttcat cttcttaaat tgctcggcgc cgatcagatg gtaattttct tcacggccat
    26401 ccacgtgaat tgaaattaca tcggctatag ccaaaagttc atcgagagaa ttgcattttt
    26461 ttgcattgcc gagcgctaat ttttcgacga tatcataata gtaaacctgc attcccatat
    26521 cttcggctaa tattgaaagt tgcgctccaa tttttccata accgactatg ccgattttct
    26581 tcccacgcac ctcgtagcag tttttggaat ttttatccca aatcccatcg tgaagtttct
    26641 gacttttgtc gaaaatgcca cgaaatagca taatgatctc tccgatagcc aattctacca
    26701 cactgcgcgt attgctaaag ggagcattaa aaacagctat tcctttctgg gaacaggctt
    26761 tcaggtctat ctgattagta ccgatgcaaa atgctccaat agccatcaaa ttttgggcat
    26821 tttccagtat ttcggcatcg ataattgtct tcgagcggat tccaagaact gagatgtctt
    26881 tgatcttctc ctttaaaact tcatgaggaa tacttccgga cagcgattca accggaaaac
    26941 cttcgttctg aaaaatacgc tgtgccgtcg gagaaatatt ttccagtaat aatacattgg
    27001 ttttactttt ctgaaatgtt acatccatat tattatccat tttttcaggc tcaaatttaa
    27061 aacaatttaa tactttttca ccgtttgagt gaatccaatt ttaaatattt ttttaattat
    27121 taaaatagtt tagcagttaa agaatttaac tcactaagta tttttctgtg atttccgata
    27181 ataaattggg tagattgatc aatatatctg gcgagatttc cgtcaatgtc agccggtaat
    27241 tcattttcat cgagcaggct tatttcacct tgcggcgaaa taaaaacatc cacgattaag
    27301 tcacgccatt caaactgaag attactcagg tgaattcggt cagcgatatt aaagtaatcg
    27361 ccgagatggc caccctgcgg agcaaaccac cgatataaag taaagggttg cgattcccaa
    27421 tagaaagcat aagtctgcgt atttttcggc aaaatttgat tattgacaac gtatggtttt
    27481 tcaagtatgt atcttaatat cccgaaatct tcatccagaa aaatcaactc gcagtcaaaa
    27541 gtccggactt caccggataa taaatgtttt acttcgcgac aggcaggata aatcatttta
    27601 ttttcgttca ggcgacaaga tgctcaatta aaattttaaa accaatggca atcaggataa
    27661 ttcctccgac tacttcgact tttttaccaa ggtaatgtcc aattcgtttg ccaattatta
    27721 ttccggtaaa tgaaataata aaggtgataa ctccaataat gatgataggt acaacaatat
    27781 taatttctag aaaagcaaaa gtaattccaa ccgccagagc gtcaatgctg gttgccagcg
    27841 ataaaccaaa tagacgatga ctattcaatg gatcgattgc attggtacat tcttttttcg
    27901 agaaagcctc ataaatcatc cgtattccaa tgaaaaccaa tagcccaaaa gcaacccaat
    27961 ggtcgaaatt cgccagaaat ctctggagag tttggcctgc cagccagccg atcagcggca
    28021 ttaaagcctg aaaaccgccg aaggctaaag ggacttttaa aaacttgaac ttattatgag
    28081 gctgagctgc taaaccgcag gtaatcgaaa tcgcaaaagc atccatcgcc agaccaattg
    28141 caattaatat taatgctaat aaattcaatt attcctgctc cgactcttct tcgttaatca
    28201 cattaagata cttaacttcc tgttccggcg gcagctcgga tttttctccc agctcgactt
    28261 tggcaatttt ctcgaatgaa ttggaaattt tattggaagt tgtatgaatg tccttgacat
    28321 ctttttgaac cgtgtctata tgagcggata atttattcca gcggtcgcga taacgacgga
    28381 actcctcgga taatttcata atttcctgtt ggataatgtc ggcatatttg cggcgctcta
    28441 tgtcacgcaa gacgagttga accgtattca aaaccgccat aaaagtagta ggcgaagtca
    28501 accaaactcg acgagaatga gaatattcta ttaaatcgtc atgataagca tataattcgg
    28561 caaaaactgc ttcggctggg ataaacataa tcgcctggtt ggaagtttcg cccggcaaaa
    28621 tgtacttatc ggcaatctcg tcaatctgct tcttcacgtc agctttgaac aactttgttg
    28681 ccgcaatacg ttcagcttcg gacaggctct tatcaatcga gcgtttgtaa ttatccagtg
    28741 tgaatttggc gtcaattgga atattgccga ttggttccgg taaaaacaag attgcatcca
    28801 cagttttgtc gttgctaagc gtatattgaa tctggaatat tttatcgttc ttttcgccaa
    28861 aaatagcata gaggacctgg ttgagctgca cctcaccaaa tatgccccgg ctttttttat
    28921 cactaagaat ttcctgaagt gagacgacat tagtcgagag gctttcgata tttttctgag
    28981 cttcatcaat ttttgccagc cgctcgatca cattcgtaaa tgtttcattg gtttttttga
    29041 aaccttcact gagattttcc tgaactttat tattgatcgc atctaatttt tcagctacgt
    29101 ttttagtcaa atgctcaaaa tcttcagata ccgccctttt gaaagtatct ttgaactgac
    29161 tgaattcttc agtctgcttt ttgcttgtct caccgagcga tttggtgatt tctacttgaa
    29221 agttgccaaa cttctcgatc atctcacgat ttttggtaga aacgttgtcg gtaaattcgg
    29281 taaaacgctt ttggacaatg tcagccgttt tcgactgaac taattccggg aaaatgttca
    29341 actgcgattc cagggcaata attttttctt ttaaagcttc cagagcggca tcgccggatt
    29401 gatccgtctt ttttctcact tttgccatta taattaccag taagctgata ataactaagc
    29461 caccgattat gataaataag tctgcattca taactattgc cttttaattt tccgttgaac
    29521 ggattttatt gaaaaatgaa tttagtaaag atcggcataa ttcagcttta actccgcctt
    29581 ttaccaaagc cttatgattg agcaggtttt ggtcgcaaag gttttcgacc gaaccgcaca
    29641 ttccagccgc atcatcatac gcaccgaaaa cgactcgtga aattcgagca tttaagatag
    29701 ccccggcaca catcggacaa ggttctagag taacatataa agtacaatcc cctaactgcc
    29761 aggaatctaa aaaagtagcc gccgaggtga tcgccaacat ttcagcatgg gcggttgggt
    29821 ctttcaagcc ttcccgctga ttatgaccct taccgatgat tttatcggcg tgaacgacga
    29881 tggc
//
LOCUS       DAOMJU010000016        29450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1400_length_29450_cov_24.644293, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000016 DAOMJU010000000
VERSION     DAOMJU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 29450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29450
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1400_length_29450_cov_24.644293"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..801
                     /locus_tag="PLO58_02795"
     CDS             196..801
                     /locus_tag="PLO58_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73748.1"
                     /translation="MRLTKNQQNALRVARLISVILHPLVIAPLTYALIIAHTSEINRT
                     QKLLYLGLVLIAIIFIPLISVLYLKKRGKTTSLDVPERDKRISPFLVCIAGYFIVWIL
                     LKVSGAPRPISLLMWCYGFNSIIATIITKYWKISVHGMALGGPIAALGFVISPTFYWG
                     ILIAPLIVYSRVKLKAHTIAQVITGFLLGFLLTLIQFGIFL"
     gene            804..2039
                     /locus_tag="PLO58_02800"
     CDS             804..2039
                     /locus_tag="PLO58_02800"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HQO73749.1"
                     /translation="MIDVLAIILGGGKGTRLYPLTKYRAKPAVPFGGKFRIIDVPISN
                     CLNSGINKIYILTQFNTHSLHRHIYSTYSLGPFSNGFVDILAAQQTMENTDWYQGTAD
                     AVRKNLPFINEIKAAYTMILSGDHLYRMDYRLFLEHHIKNKAEITVAVKPVSRQDAGH
                     FGILQTDEKCRIVQFVEKPNSELLDKLKSPGLPDETPFLASMGIYIFATETLHKILEQ
                     NPSDDFGKNIIPASIPTLNVCAYQFDGYWRDIGTIESFFTANLELARFQPPFSLYDEH
                     APIYTHARSLPASRLENCSLTQTLLADGCHIGESKISESVVGIRTIIQKGTTIEKCVI
                     MGADYFEQVQPEGSPQLGIGQNCVIKNAIIDKNARIGNNVQIVNRDQLKEYESELFSI
                     RDGIVVIPKNTTIPEGMII"
     gene            2052..2870
                     /locus_tag="PLO58_02805"
     CDS             2052..2870
                     /locus_tag="PLO58_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73750.1"
                     /translation="MYKNYDPQQMPLFPDLFEDIYLPPITTLPEFNQEIDNFIKLADF
                     GALIDLTFQGLDKSYSLRLADFAIPAQMIRDDARPESPIFNLFPPEHHRRLQRFRYEI
                     KAFFNNQNSIKTPNGYFLLRSHFQQWENYRGQFYQKIVNYLQNDIGPRRYRNFFETCF
                     KSGLEWLNAQLRPIHPYQVQFTDLQEISRRRKSLQSTRTTLAQLVPEDPEYLLNCITL
                     KTLHIPLTLAQFIKGVAINSTFKTIHLNYLKDVKIETLRDVKHLLESISRQSDQ"
     gene            2867..3538
                     /locus_tag="PLO58_02810"
     CDS             2867..3538
                     /locus_tag="PLO58_02810"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQO73751.1"
                     /translation="MNNFSPDNCQTADKMVAEQILARNIRDPELLEAFRQVPRHLFVD
                     DTQRTLAYSDQPLPIGYGQTISQPYVVAFMIAALQIDKSAWVLEIGTGSGYETAILAR
                     LAGNVFTIERIGELSQRAQKVLSQIGIQNVEFRIGNGCLGWPEKAPFSHIIVTAASPE
                     IPPALVDQLALGGRLVIPLGSSCGGQELTLIEKTPLGLNSQKILPVRFVPLIWDSSNT
                     KPFIK"
     gene            3548..4363
                     /locus_tag="PLO58_02815"
     CDS             3548..4363
                     /locus_tag="PLO58_02815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQO73752.1"
                     /translation="MKDWDQNLFEEFVSQIEAVSRGEYNDESFKKFMRIGVPNSLRQL
                     ATILDNLLRRFETRELHLKEAIEELKSARSDLQDLNTLLDSRVRERTQALEEANRRLE
                     ALSTTDALTGIYNRRYFDQELKREAARSIRYNSPLSLLMLDIDLFKIVNDTHGHLFGD
                     EVLKTLGQILLTCLRKNDFCARYGGEEFAVLLPETGEKEAFLVAEKIRTIVAKRKIYF
                     EQAVVSITISIGIAQFNPAGMIDETQLVDAADKALYQAKRSGRNKSVIFDYSV"
     gene            4463..6706
                     /locus_tag="PLO58_02820"
     CDS             4463..6706
                     /locus_tag="PLO58_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQO73753.1"
                     /translation="MRYFPLASVIVLLFQTTLSQNYLWPTNSSRLISSTFGEYRVNHL
                     HAGLDIKTNQRTGYPVFAIENGYIKTIRTSFTGYGKVIYHQLDDGNIAVYAHLDDFAE
                     PLSTIVKAEQERQKRYRIEVNLKQDDLRVTKGQIIGYTGETGTLHPHLHFELRDSLDN
                     PFNPLNTNLTVNDKTAPTISGIAVTPISLDARINGLPMTQTFKTIQKKINHHILKKPI
                     QVEGQFGIEIKVHDTIKGVPNVYPPYGIKLFIDDSLYFQVQYDRFSYEQTHFAIIDRN
                     YQLDHDLGEVYNRLWTIAPHKNMPMNTLPEATGIIDLETGLHSVRIIAYDKNQNQAMV
                     DFKILSAPVTSFESVEIQTGEEGYQLFLSRPAQPIVENFKARWVNKNGHYLRQANLAL
                     TDTTAEFYKLSLIDQPVDNEYLKIEAVGVDGNQFQPLFINLNQAEKSSVISLNYTFIH
                     NPKNFLMLLTFSAVLDSNPTFDLQTSNGLGKVELIRISPTEYMTVPVPFAYWRDASAY
                     EIRFDNQLTNIFRGRLNLKCIVPSNENFCLSQDSVFKAVFPRDAVFDSLLVWLETKEA
                     TEIKGGKMVSARYGLHPTNQPLHDSIQVCFKNPVCAQDISQVGIYQYVDKNWQFRGNQ
                     LDVNQDFIWAYSRQTGEFALIKDYTPPAIRNIFPGNGGRFHANDVHYLKATVHDQLSG
                     ITDDQSIVVTLDGQPIIAEYNAPKNQIRYRLSRQLTAGKHALSITITDRAGNVKTAVS
                     TFTIIPG"
     gene            6715..7407
                     /locus_tag="PLO58_02825"
     CDS             6715..7407
                     /locus_tag="PLO58_02825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQO73754.1"
                     /translation="MFIPLRDSTMRLTPPYVTVGLITLNCLVYIYQLTLPPEILVAFL
                     NTYGLVSANLFNHPSLRDFLSFFTTLFLHAGFLHLFGNMLYLWIFGDNIESTLGHGRF
                     LLFYILCGVIAGLTQVLITPRSTIPMIGASGAISGVLGAYLLRYPHARVKVLFWFFIF
                     IRQIWIPAGYLLGFWFIVQLSNGLGSLGFQTQGGVAWFAHIGGFLAGIILLLILEPYE
                     RKRMWKKLNQNY"
     gene            7380..7787
                     /gene="cdd"
                     /locus_tag="PLO58_02830"
     CDS             7380..7787
                     /gene="cdd"
                     /locus_tag="PLO58_02830"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQO73755.1"
                     /translation="MEKIKPELLKVAARQAANNAYAPYSHFPVGAALLTNDGKIYTGC
                     NVEASSYGLTVCAERNAIAAAISAGERNFRAMGIFSLTRVSPCGACRQVIWDICGDID
                     IFLFDESEDYQHFRSSELFPIPFDNNQMKGIDK"
     gene            7788..8426
                     /gene="udk"
                     /locus_tag="PLO58_02835"
     CDS             7788..8426
                     /gene="udk"
                     /locus_tag="PLO58_02835"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HQO73756.1"
                     /translation="MKKAILIGVAGGTGSGKTSMARNIVHDFDPRDVVIIEQDSYYYD
                     LSDIPLESRGHHNFDHPDAYDFPQLRADMSAILKGQQVKIPIYDYKTHTRSKTESQVV
                     NGQKIIVLEGIMVLYDPELRDMMDIKVYIETAPDIRFIRRLRRDIHQRGRTVDSVIAQ
                     YLETVRPMHEQFVEPTKHFADIIIPEGGQNSVAIDLFKTKVRSLLRHLNEPR"
     gene            8438..9058
                     /locus_tag="PLO58_02840"
     CDS             8438..9058
                     /locus_tag="PLO58_02840"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HQO73757.1"
                     /translation="MSQPNLHRVPTDFEEVVAITEKSAGWIEVICGSMFSGKTEELIR
                     RLRRAQIARQRVAIFKPVIDVRYSEDYIVSHNQQKIPSIPIHTAQEILQKAGNAQVLG
                     IDEAQFLDNELVAVCRTLANSGRRVIVAGLDKDYRGEPFEPIPQLLCEAEYITKTLAI
                     CMKCGNPANFTQRIIKSSERVLLGATDIYEARCRACFEPPIIEEKK"
     gene            9102..10736
                     /locus_tag="PLO58_02845"
     CDS             9102..10736
                     /locus_tag="PLO58_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HQO73758.1"
                     /translation="MKYIGIIGLLVILGIAFLMSNNRRLISLRVVIWGLLLQLLFAGI
                     ILGKMLISYFTLFIFLLLIIIFIHKDQINTVGHRSKQWLYIGLISIATVLAVAAFYWF
                     GKTGWLAELIGLITVAIVIKAIFKVRPWQKELTVAWLTAGLSWLILHGYTGQDIFAFL
                     SQKVEKFLGLATLGAEFLFGNLALDKYFFPNETTWPGFGMQFAFTVLPVIIFFASFMA
                     ILYHLGVMQAIIKAMARFMQWTMGTSGAETLSCTANIFVGQTEAPLLIRPYVDQLTLS
                     ELLTIMVGGFATIAGGVMAGYIKMGIPAGHLIAASVMSAPAALVIAKIIYPETDHPQT
                     AGDVDLPEDTVKSANLLDAAARGVTDGLKLAATVGAMLIAFIALIGLVDTILGFIDRL
                     IDGNLLKGAYTIYTNSSGFSPVKGEFSGIFPGSLKSLFSFILRPLAWLMGVSWQYAGE
                     VGNLMGIKISLNEFVAYSALSELIHTGALDQKSIVIATYALCGFANFASIGIQIGGIS
                     SLAPGRRSDLSKVALKAMFGGALASWMTATIAGLLL"
     gene            10793..11437
                     /locus_tag="PLO58_02850"
     CDS             10793..11437
                     /locus_tag="PLO58_02850"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQO73759.1"
                     /translation="MKVILLGPPGVGKGTQAQKLQQERGLRQISTGDILRQAIKNQTE
                     LGKQAEDYIKRGELVPDDLMLGLIAETLFGDDAPQDYILDGFPRTIPQAEGLDRLFAQ
                     HNVDLDAVILLEAEPEAIIKRLSARRTCRQCNAVYNLITNPPKNPLRCDICGGELYQR
                     DDDRIETIQRRLKVYTKQTEPLAEYYRQRGKLKTIAADGTPDEVFQRINRVIGG"
     gene            11444..12031
                     /gene="coaE"
                     /locus_tag="PLO58_02855"
     CDS             11444..12031
                     /gene="coaE"
                     /locus_tag="PLO58_02855"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQO73760.1"
                     /translation="MVTLGVTGGLGSGKSTACKFLAEKGAYVMDADLIAKQLMENDPQ
                     VRDEIIAAFGNDVYKDGKLDTQKLAHRAFASEADQRILNDIVHPRVIDYFQQEVDKMR
                     DKYDLIVLDAPLIFESGFDSRLDHTLLIYTKYKYRLERALRRGNLTREDILRRIELQM
                     PEEDKRELAEFVIENNGTEEQLKSAIEKLYDKLTI"
     gene            12402..14009
                     /locus_tag="PLO58_02860"
     CDS             12402..14009
                     /locus_tag="PLO58_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HQO73761.1"
                     /translation="MKQATFSWYDGLIFILFFLFVVIFSMYKSRKGKTGEDYFLAGRQ
                     LTWPLIGLSMIAANISTEQFVGMAGQAAGSAGLAIASYEWMAAITMVIVALFLLPNFL
                     RTGIYTFPEFLEYRYSPLARRLMAVYTILIYVFVLVASVIYTGGLTIVTLFSGMKFFG
                     VEVTLIRAIWLIGIITAIYTIWGGLKGIAWADLFFGSSLIFGGFLTLIIGLRHVGGLS
                     SFLEANQDKLHMILPRDNPVLPWTALVIGLWLPNFYYWSVNQFITQRTLAAKNLREGQ
                     LGVIFASFLKLITPFIIVFPGIMAWQLFKDQMTGPGGTDAAFPTIVRNLIPNGFLGFI
                     FAAIGGAVISSLASMLNSASTIFTMDIYQHAVKKELPQQKIVLVGRIATFIFLIIGCL
                     IAPILDNPSLKGIFTYLQEFQGFISPGILAAFAFGLIFKRAPKAAGLAALILNPIIYG
                     LLLIFCGSLPVFASVQIVPIAFLNRMAITFIIIVITMGIITWLKPLDQPVKMPERKDF
                     NMQSSPIVKWLGLTVIGLVIVLYIIFW"
     gene            14041..15090
                     /locus_tag="PLO58_02865"
     CDS             14041..15090
                     /locus_tag="PLO58_02865"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HQO73762.1"
                     /translation="MPVDFSQIPHRRFNPLTGEWILVSPHRTERPWHGSQEPVASEKR
                     PEYDPECYLCPGNRRASGTVNPLYTSTYVFTNDFPALFPDTPHRRRNVRKYIRDESVS
                     GTCRVICFSPRHDLTLAEMAVEEIRDVITVWAEQTKELGENFSWVQIFENKGAMMGCS
                     NPHPHGQIWAVNSLPNEPYKENRRQSAYFKQNKSVLLTDYLDYELESRERVVLENENW
                     LAVVPFWAIWPFEILLLPRRPILRLPDLTPAERNSLALILKSLLTKYDNLFQSSFPYT
                     MGWHGAPTMPGNFDYWQLHAHFYPPLLRSSSVKKFLVGYEMLAEAQRDLTPEVASRTL
                     RETSEIHYKSLIKNK"
     gene            15100..16398
                     /locus_tag="PLO58_02870"
     CDS             15100..16398
                     /locus_tag="PLO58_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HQO73763.1"
                     /translation="MPKNQITNSPQNQIDTTVFQKIYGHNPSVIAEQIKRYTYLADRH
                     NEIFAASPALFFSAPGRTEISGNHTDHNHGKVLAASVDLDSIAAVNAINGDQITIFSE
                     GYPQPFIVDINHCSPVETERGTTTALVRGIVARFKELGLRVGGFNATLTSNVLVGSGL
                     SSSASIEVLLGTIMNYLFNDGQIAAEDIALIGQFAENQYFGKPCGLMDQLAIAVGGVV
                     AIDFKNPSKPIIQKVNYDPEQQNYSWLIVNTGTSHVNLTEEYAAIPREMRAVARALGG
                     QVCRDITMQNLIEEIPNLRPKVGDRAILRAFHFLTENERVQQQISALEKGDFAAFLEL
                     ANESGTSSCRWLQNCYPTSNPTEQGVPLALALTQYFLKTVGKKGACRIQGGGFAGTIL
                     VFIPNEVLSDYLKLITKVFNEGSVSQLNIRPIGAVCLNKI"
     assembly_gap    16817..16916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16917..18965
                     /locus_tag="PLO58_02875"
     CDS             <16917..18965
                     /locus_tag="PLO58_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HQO73764.1"
                     /translation="ESNWTEATFRFDDGINQPPLPPVEGFNPDGIIVASHYPELKWSP
                     AIDPDVADRLRYEIVLSRDRLFGSRTYILLKSTYDQNQIVVQTPLLENCQYFWRVRSI
                     DLAEARSVWSKSNTFWVNQINEAPQRPVRIIHPHNLEEITPNSYFWWLPSDDPDPGDQ
                     LNYQIECSESLNFTEPIFSYRIPRTITAKEVETKLELPQRAIGVCLDEISAISLLKDN
                     TLYYWRIRAFDQSEISGTSASRPVRVAFNYQNDPPLPVVKGFYPADDVIIKTLRPEIR
                     WANSSDPDFSDFQASLTYQLEISRDPQFPENACSVYTTEPGQTVFTLPENLIENERWF
                     YRVRACDQHGSYSLWSPTNSFITNAISEAPYPVTDGFLPKDSAVVETAQPLISWLPSD
                     DPDPDQDERDLYYLVRFYEADNPKKVSQVASKTGITSLHLPELKEDKYYFYQISAVDP
                     DGNRSEWSQLVCFGVNAVDLPPGPFALLAPFNGQDSVALDAGFVWHIAHDKDPGSQIN
                     YTLYYGTDSLFLTNFREIMFEQPEEDTIMVYNPPERLNYATRYFWKVVATDNRGNQCW
                     ASQTNQRPFVFTTVGTQRSIASIFKNFRLHQNYPNPFNIETLIRYDVPFYGPVDVSIY
                     DLIGMKVTTLATGNHAAGSYSVYWNGTDQNGTLVANGVYICRLTAPGYMSHIKVVLMR
                     "
     gene            complement(19256..19387)
                     /locus_tag="PLO58_02880"
     CDS             complement(19256..19387)
                     /locus_tag="PLO58_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73765.1"
                     /translation="MTTESENGNPKEREAELIQKIRNRVAEVQELVDEMKSRLTDKK"
     assembly_gap    19549..19648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19780..22419)
                     /locus_tag="PLO58_02885"
     CDS             complement(19780..22419)
                     /locus_tag="PLO58_02885"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HQO73766.1"
                     /translation="MKKFKVVANLCFLTICLSGITMGNTQVKATRTDESIRIDGILSE
                     EIWQRAIPAPPLIQDEPNEGAQPSQPSDIWIVYDDEALYVGARLYDSAPDSIVARLGR
                     RDSDWNTDLFLIALSPFNDKRTAYFFGVSAAGAIYDGKFYNDNWEDDTWDGVWEGAAQ
                     RNHEGWTAEIRIPFSQLRFQKSDQYVWGVALYRKIQRKNEEDMSFFTPRNESGMVSRF
                     IELVGIEGINAERKIEVLPYIRGKGEFLAVEPEDPFNDGSRYFASTGADFKIGVGNNL
                     TVDGTINPDFGQVEVDPAVINLSDVETYYNEKRPFFIEGSDIFNFGYGGSNNYWNMNF
                     STPSIFYSRRIGRAPQRDLSDYDYTDVPDGTRIISAGKLTGKVGDNWNVGLLNALTAR
                     EFARVSPNGLDSKIEAEPLTNYAILRTQKEFQEGRHGLGIITTSTKRFFRDESLADYL
                     NSDALVVGLDGWTFLDTNKTWVIAGWGAMSNIQGNENCITDVQTNARHYFQRPDAEHL
                     GVDSNATALTGFATRINLNKQRGNVIFNAAFGLIDPRFEVSDLGYLSRGDVINYHVGA
                     GYQWTEPNHFYRKFNLILAHTLGMDCGGNLTNNGIMNLGYIQFSNYYWIDYGSGYFWE
                     TYDTRLTRGGPIAISPSGRWGYIQISTDERKPLVGRLYYETSGNDTASVSHYLQIPLD
                     WKVSDNLAFELSPSISINHNYAQYVDVFDDPYADATYGKRYVFAKMDQKTISASLRLD
                     WTFTPRLTLQLYAQPLYGAAEYYDFKEFARPKTYQFFTYEEGTEINRNSDGNYTVDPD
                     GNGPAGSFSFDNPDFNVYSFRSSAVLRWEFALGSAIYLVWTHGRFENSSEADFDLSRS
                     VNRIGKIKPDNIFMIKASYWFNV"
     gene            complement(22564..25260)
                     /locus_tag="PLO58_02890"
     CDS             complement(22564..25260)
                     /locus_tag="PLO58_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQO73767.1"
                     /translation="MNTKKRPYEDIAEELVCESEDKLASLVRNLPGAAYRCLYDAERT
                     MIFISDGCRSLTGYSPDDFLMDHKITYSQIIHPNDRQYVFDEIGASLKKRSPYQLLYR
                     IITADNQEKWVCEQGVGIYNGDENLVVLEGFITDISEKVKANIALQSYETRFQSIWEG
                     SFDGMRLTDKDGKIIAVNKAFCRQVGMRRNELENASIGIIYPPEERQEIETNYRNNFS
                     RRHIKSLTECESILWNGQKVWFEISNSFLDIPGEQVYLLSIFRDITKRKNAEAELAES
                     ARRFRWLYEYAPIAYHTITPDGIIVDVNRQWCEIMGYSKEEVCGRPIFDFVIESERES
                     ARKSFDEKKLSKKTSSTGNERHYLTKSGEVKTFSISDYIIRDDDGRIRSIQTTMSDIS
                     ELKRTEEALRMSEAKFRHLVESSLDVIWQADDSGRVLYVSPNIKDILGRTPDKLIGKQ
                     FADILPKGSEEKGGKIIASHFTKRKPIKNLELTIVNHLGQEIILEVNGVPNYDGGKKF
                     TGYIGTMRNVTIQKIVEDEQIKVQKLESLSILAGGIAHDFNNMLSGILGNISLAKMKV
                     KDSDITRILERSEKAALRSRSLTQQLLTFAKGGAPVMESIQVESFLRELVEFAMVGSS
                     VNCQFDIAPDLPNVEADRGQLSQVIQNLVINAIQAMPSGGNLKISANAEEIKTDGLVP
                     IKSGYYLKIAIQDTGVGIPKNLLSKVFDPYFTTKPHGNGLGLSVVYSILKKHDAYIFV
                     DSEAGKGTTFTVYLPATAAVQKGSQKAEPQISKGNGRILVMDDDELICEVVEEMISSM
                     GYDVKCVPNGEEAIKSYKEAKEAGQPFDLVIMDLTIVGGMGGKETIAELKKFDPDVKA
                     LVSSGYINDSMMTVPQKYGFIDIIAKPYRMEDLSSILKEHVG"
     gene            complement(25696..26088)
                     /locus_tag="PLO58_02895"
     CDS             complement(25696..26088)
                     /locus_tag="PLO58_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HQO73768.1"
                     /translation="MTILGNILWIFLGGGILIFFFYLFGGVVLCLTIIGIPFGMQCIK
                     LSVLGLMPFGQKIVYKASEPGCLSTVMNIIWLLFGGLELAVVHLVFAALCAITIIGIP
                     FARQHIKLLSLALTPFGREVQPIQKDRT"
     gene            complement(26227..26658)
                     /locus_tag="PLO58_02900"
     CDS             complement(26227..26658)
                     /locus_tag="PLO58_02900"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HQO73769.1"
                     /translation="MSINETKIVVCPHCQHEQSVKIWQSLNTALDKEVKKELFEGKIN
                     LLVCENCQQKTFIPVPFLYIDPERQIAVHYFPTAVIRDEKFLQQFNIDGTYAGGESTG
                     LEVPDYMRYLHIVFNMDELVTFIVFREVLFDFHRTGKVLLD"
     gene            complement(26682..28808)
                     /locus_tag="PLO58_02905"
     CDS             complement(26682..28808)
                     /locus_tag="PLO58_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQO73770.1"
                     /translation="MNFRKQLRTSISAISLALAIWLSGCPSRPLTPPTAKIEPKTTII
                     HSDTLIDNYAWLREKSNPEVIQYLEDENAYTEAIMKPTRRFQKKLYKEMLARIKETDE
                     SVPVKIDSFYYYNRMVKGNQYPLYCRKKGSLDANEEILLDQNRLASGQKYCSIGEFKV
                     SPDHSLLAFAVDFNGSEKHMLVFKDLINGQMLPDTILDVSYGLEWANDNRTVFYTTLD
                     TILRPDKLWRHQLSQSPENDVMVFHEPDEKFYLGIAKTRSHGYLILTLGSQITTEVHY
                     LSADQPDGRFRLFCPRKTGVEYYIDHHSDKFYILTNENAINFRLLETPIARPEKHNWQ
                     EKIPHRPNVMLTGIDLFCDHLVVYERENGLEQIRVENLNTGATHYIDFPEPVYTIDSD
                     NNPDYNTKILRFRYTSLVTPNSVYDYDMESQTRELKKQDEVLGGYDQTQYQSERIYAV
                     AKDGVRIPISLVYKKGMKKDGSNPFLLYGYGAYGIPSEARFSSIRLSLLDRGFIYAIA
                     HVRGGGELGRQWYEDGKLLRKRNTFTDFIACAEMLIAEKYTSAEKLVINGGSAGGLLM
                     GAVVNMRPELFEVVVAEVPFVDLINTMLDRSIPLTVIEWEEWGNPHLEKYYNYMKSYS
                     PYDNVGPKAYPHMLITAGLNDPRVAYWEPAKWTAKLRAMKTDDNVLLLKTDMGKGHFS
                     ASGRYDYLKEVAFKYAFIFAKLGIRE"
     gene            complement(28821..29309)
                     /locus_tag="PLO58_02910"
     CDS             complement(28821..29309)
                     /locus_tag="PLO58_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007984056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQO73771.1"
                     /translation="MAQNNAEILIRSARLSDADDLVAFNRAMAQETENRILSAEVISA
                     GVRNLFQHPEWGFYIVAESDGRMVGCLMITYEWSDWRNGLFWWIQSVYVPPEFRRRGI
                     YRKMYDYVQNAALKVGNVCGLRLYVERDNRIAQKTYRKMGMSKTDYILFETTELAKRT
                     NK"
BASE COUNT         8127 a         6651 c         6048 g         8424 t
ORIGIN      
        1 attagtcggg cagggtggtt aggacaccac gctttcaata gtaataatca aatagtcaat
       61 taattgagcg cttttacaaa ctttatgttt tttctcgtga tttatttgtt gaaattgata
      121 taaaaagaac gtataatttt acggaatgaa atataataga ttattgattc ataactatcc
      181 cagactatag tcattatgcg attgactaaa aatcaacaaa atgcacttcg agtagcccgt
      241 ttaatctcgg taattctgca tccactcgtc atagcgccgc tgacctatgc tttgattatt
      301 gcgcatactt ccgaaatcaa tcgaactcaa aaattgctat atttagggtt agtgcttatc
      361 gccatcattt ttatcccact catttctgtc ctatacttaa aaaaaagagg gaagacgacc
      421 agcttagacg ttcctgaaag agataaacgg attagtccct ttttagtctg tatcgcaggt
      481 tattttattg tatggatttt gttaaaagtg tctggggcac cgcgccctat ttctttacta
      541 atgtggtgct atggatttaa ttcaattatt gccactatta tcaccaaata ctggaaaata
      601 agtgtgcatg gtatggcatt gggcggtcct atagcggctt taggctttgt aatttcaccg
      661 actttttact ggggtatttt gatcgctcca ttgatcgtct attcgcgagt gaagttgaag
      721 gctcacacta tcgctcaagt aattacgggt tttctattag gatttttatt aactttaatc
      781 caattcggga tttttttatg aaaatcattg acgttttggc aattattctc ggcgggggca
      841 aaggcacacg gctttatcct ttaaccaaat atcgagccaa gccagctgtt cctttcggtg
      901 gcaaattccg cattattgat gtaccgatta gcaattgcct caatagcgga atcaataaaa
      961 tctacattct tacccaattc aatactcatt cgcttcatcg tcatatttac agcacttata
     1021 gcttaggtcc ctttagtaat ggttttgttg atattttggc ggctcaacag actatggaaa
     1081 acactgactg gtatcaggga actgctgatg ccgttcggaa aaaccttccg ttcatcaatg
     1141 aaatcaaagc cgcctatact atgattcttt ctggtgacca cctctaccgt atggattacc
     1201 gtttgtttct ggaacatcat atcaagaata aggcggaaat tacggtagcg gtcaagccgg
     1261 tttcacgaca agatgccgga catttcggca ttttgcagac cgatgaaaaa tgtcgtatag
     1321 ttcaatttgt ggaaaaaccc aattcggagc ttcttgataa gttaaaatct cccggcttac
     1381 cagacgagac accctttcta gcttcgatgg gcatctatat ttttgccacg gaaacccttc
     1441 ataaaattct cgaacaaaat ccgagcgacg atttcggtaa aaatatcata cccgcttcaa
     1501 ttcctacgct taatgtctgt gcctatcaat ttgatggcta ctggcgggat attggtacca
     1561 ttgaatcgtt ttttaccgcc aatcttgaac tcgccagatt ccaaccgcct ttttctttat
     1621 acgatgaaca cgctcctatt tatactcatg cccgctcctt accggcttcc cgattagaga
     1681 attgttcact gactcaaact ctgctggcag acggctgtca tatcggtgag agcaagatta
     1741 gcgaatctgt tgtcggtatt agaacgatta tccagaaagg taccaccatt gagaaatgcg
     1801 tcattatggg agctgattac ttcgaacagg ttcagccaga aggttcgcct caattaggaa
     1861 ttggccaaaa ttgtgtaatt aaaaatgcta taattgacaa gaatgcccgc atcggtaaca
     1921 atgtccagat tgtaaatcga gatcaactaa aagaatatga gagtgagctc ttctcgatcc
     1981 gggatgggat tgtcgttatt cctaaaaaca ctacaatccc ggagggaatg ataatttaac
     2041 aggaatccgt aatgtataag aattacgatc cccaacagat gcctcttttc cccgatttat
     2101 tcgaagacat ttatctgccg ccgattacca ccctgcccga attcaaccag gaaattgaca
     2161 acttcattaa actagccgat tttggcgctt tgattgattt gacttttcaa ggattggaca
     2221 aatcatattc gctccgactc gctgacttcg cgattccagc tcaaatgatt cgcgatgatg
     2281 ctcgtccaga atcgccaatc tttaaccttt tccctcccga acatcatcgt cgacttcagc
     2341 gctttcgcta tgaaattaaa gcctttttca ataatcaaaa cagtatcaag acgcccaacg
     2401 gctacttttt acttcgcagt cattttcaac agtgggaaaa ttatcgaggt caattttatc
     2461 aaaagatagt gaattacctt cagaatgata ttgggccgcg ccgttaccgc aatttttttg
     2521 aaacctgctt caaatccggg ctggagtggt tgaatgctca actgcgtccg attcatcctt
     2581 accaggtcca attcacagac ctacaggaga tcagccggcg gcgtaaatca ttacagagca
     2641 ccagaactac tttagcgcag ttggtacctg aagatcctga ataccttcta aattgcatca
     2701 ccttgaaaac tttgcatatt ccgctgacgc tcgcccaatt tatcaaagga gttgccatca
     2761 actcgacttt taagacaatt catctaaatt atctgaaaga cgtcaaaatc gagactctac
     2821 gcgacgtaaa acatctcctg gaatctattt cgcgtcaatc ggaccaatga acaatttttc
     2881 accagataat tgccagacag ccgacaaaat ggtcgccgaa caaattttag cccgtaatat
     2941 tagagacccg gaattattgg aggcttttcg acaggtaccg cgtcacctct ttgttgatga
     3001 cactcaacga acgctggcct atagtgacca gcccctgcca attggatacg gtcagactat
     3061 ttctcagcct tatgtggtag cctttatgat tgccgctttg caaattgata aatcagcttg
     3121 ggtactggaa atcggcaccg gtagtggcta cgaaacagca attctagccc gattagccgg
     3181 caatgtattc accattgagc gcatcggtga actttctcag cgcgctcaaa aagtactgtc
     3241 ccagatcggc atccaaaacg tagaattccg aatcggaaat ggctgtcttg gctggcccga
     3301 gaaagctcca tttagtcata taatcgtcac agccgccagt cccgaaatac caccggcatt
     3361 agttgaccag ctggcattgg gcggaaggct ggtgattcct ctcggaagtt cctgcggcgg
     3421 tcaggaattg actttaatcg aaaaaacacc gctgggattg aacagccaga agattctgcc
     3481 ggtcagattt gttccgttga tatgggattc cagtaacaca aaaccattca taaaataact
     3541 gtcgaaaatg aaagattggg accagaattt gttcgaagaa ttcgtcagcc agattgaggc
     3601 agtctcacgt ggcgaatata acgatgaatc ctttaagaaa tttatgcgaa tcggcgtacc
     3661 gaattccttg cgccaattgg caacgatact cgacaatcta cttcggaggt ttgagactcg
     3721 cgagttacac ctaaaagagg ctatcgaaga gcttaaatcg gcccgctcgg atctgcagga
     3781 tttaaatact ctgctggata gccgcgttcg ggaacgcact caagccttag aagaagccaa
     3841 tcggcggcta gaagcgctct cgactaccga tgctctaacc ggtatttata accgccgcta
     3901 tttcgaccag gaactaaaac gagaagcggc tcgtagtatc cgctacaatt caccgttgag
     3961 cctactgatg cttgatattg atctcttcaa aattgtcaat gacactcacg gacatctctt
     4021 cggtgatgag gttttaaaaa cattgggtca gattttgttg acctgtttac gcaaaaatga
     4081 cttttgtgcc cgttatggcg gagaggaatt tgccgtccta ttgccggaga cgggagaaaa
     4141 ggaagctttc ttagtagctg aaaaaatccg tacgatcgtc gccaaaagga aaatttattt
     4201 tgaacaggct gttgtcagta ttacaatttc catcggtatc gctcagttta atcccgctgg
     4261 gatgattgat gaaacccaat tggttgatgc cgccgataaa gccctttatc aagccaaacg
     4321 gagtgggcgt aataaatcgg taatctttga ttattccgtt tgagcctaat tgaaatgcta
     4381 ccgattgctt actaaataaa atttgaaaat tggagctaat ttttcttata ttccaccccg
     4441 aaatgaaaat tagttttacg gcatgagata tttcccactc gcctcggtta ttgttctcct
     4501 ttttcaaacc acactttctc agaattacct ctggccaact aactccagcc ggcttattag
     4561 ttcgactttc ggcgaatatc gtgtcaatca tctacacgcc ggcctcgata ttaaaaccaa
     4621 ccaacgcacc ggctatccgg tttttgccat cgagaacggt tatatcaaaa ctattcgcac
     4681 ctcgtttacg ggctatggca aagtaattta ccaccaactg gatgacggca acatcgcggt
     4741 ttacgctcat ctggacgatt tcgcagagcc actctcaact attgtaaagg ctgaacagga
     4801 gcgtcagaag cgttaccgca ttgaagttaa tcttaagcaa gatgatttgc gcgttaccaa
     4861 gggacaaatc atcggatata ccggcgaaac tgggactctt catccacacc tacattttga
     4921 actccgcgat agcctggata atccttttaa tcctctgaac actaatctta ctgtgaacga
     4981 taaaactgcc ccaaccatca gcggcattgc cgttacgccc ataagtctcg atgctcgtat
     5041 taacggattg ccgatgactc aaacctttaa aactattcaa aagaaaatta atcaccacat
     5101 tcttaaaaag ccgattcaag ttgaaggaca attcggaata gagattaaag ttcacgatac
     5161 gatcaagggt gtcccaaacg tttacccgcc ttacggtatt aaactattta ttgacgacag
     5221 cctctatttc caggttcagt atgaccgatt cagctacgag caaactcact ttgccatcat
     5281 agaccgcaat taccagctgg atcatgacct gggtgaagtg tacaatcgtt tatggacaat
     5341 cgcaccgcat aaaaatatgc caatgaatac cctgccggag gctaccggta ttattgatct
     5401 ggagaccgga ttacattcag tgcgcataat tgcttatgat aaaaatcaga atcaggctat
     5461 ggtggacttc aaaatccttt ccgctcctgt tacttccttt gaatcggtag aaattcaaac
     5521 cggcgaagaa ggttatcaac tgtttctatc gcgtcccgcc cagccgattg tagaaaattt
     5581 taaagctcgc tgggttaata aaaacggcca ttatcttcgc caggctaatc ttgcattaac
     5641 tgatacgact gcggaatttt ataagttatc attgattgat caaccagtgg ataatgaata
     5701 tcttaaaatc gaggcggttg gcgttgacgg caatcaattt caaccattat ttattaactt
     5761 gaatcaagcc gaaaaatcct cggtgatttc attgaattat acattcatcc acaatcccaa
     5821 aaatttcttg atgctcctga ctttcagcgc cgttctcgat agcaatccga ctttcgatct
     5881 tcagaccagt aacgggctgg ggaaagtcga gctaatccgg atttccccga ccgaatatat
     5941 gactgtgcca gtgccctttg cctattggcg cgacgcctct gcctatgaaa ttcgctttga
     6001 caatcaatta actaatatct ttcgtgggcg attaaatctt aaatgcatag tacccagcaa
     6061 tgaaaatttc tgccttagcc aagactcggt cttcaaagca gtttttccca gagatgcggt
     6121 cttcgattca ctgcttgtct ggctggaaac taaagaggct accgagatca aaggcggcaa
     6181 aatggtatcg gcgcgttatg gacttcatcc cactaaccag cctttgcacg attcaattca
     6241 ggtatgcttt aaaaatcctg tatgtgctca ggacatcagt caagttggaa tttatcagta
     6301 cgttgataaa aactggcagt ttcggggaaa ccaactcgat gtcaaccagg attttatctg
     6361 ggcttatagc cgacagaccg gtgaatttgc tcttataaaa gactatacgc cgccagctat
     6421 tcgcaacatt tttccaggca atggtggccg gtttcacgcc aatgatgtgc attatctcaa
     6481 ggctactgtt cacgatcaac tctcaggcat tactgatgat caatccatcg ttgttacgct
     6541 tgatgggcaa ccaataatcg ccgagtataa tgccccaaag aatcagatta gatatcgcct
     6601 gagccgacaa cttacggccg gcaaacacgc cttatccatt accattaccg accgggctgg
     6661 caatgtaaaa actgcagtga gtacttttac gataatacca ggttaagaga aataatgttc
     6721 atcccacttc gcgattctac tatgcgcctg actcctcctt acgttactgt cggacttatt
     6781 actttgaatt gcctcgtata tatttaccag cttactttgc caccggagat tttagttgca
     6841 ttcctcaata cgtatggatt ggtatctgcc aatctattta atcaccccag cctgagagat
     6901 tttttatcat ttttcacgac gcttttctta catgctggct ttctccatct ttttggtaat
     6961 atgctctacc tatggatttt tggtgacaat atagagtcaa ccctgggaca cggtaggttt
     7021 ttgttatttt atatactctg cggagtaatt gccggtttaa ctcaggtgtt aattactcct
     7081 agatctacga taccgatgat aggtgccagt ggtgctattt ccggagtttt gggagcttat
     7141 ctcctgcgtt atccgcacgc tcgggtaaag gttctcttct ggttttttat attcattcgt
     7201 cagatctgga ttcctgctgg ttatttgctg ggattctggt ttattgtcca gctcagcaat
     7261 ggtttaggct cattaggatt tcaaacgcag ggtggagtgg cgtggttcgc ccatattggt
     7321 ggctttttag ccggaataat tttattgcta attctggaac cctatgaaag gaaaagaatg
     7381 tggaaaaaat taaaccagaa ttactaaaag tcgcagctag gcaagccgcc aataacgctt
     7441 atgctcccta ctcgcacttc ccggtcggcg cggcactttt aactaatgac ggcaagattt
     7501 acaccggttg taatgtagaa gccagctcgt acggcttgac agtatgcgcc gaacgcaatg
     7561 cgattgcggc ggcaatttca gccggtgaaa gaaattttcg tgctatggga attttttcgc
     7621 taacccgggt atcaccctgc ggtgcctgtc ggcaggtaat ctgggatatt tgcggcgaca
     7681 ttgatatttt tctttttgat gaatcggagg actatcagca tttccgcagt tccgagctgt
     7741 tcccgatacc attcgataac aaccagatga agggaattga taaataaatg aaaaaagcaa
     7801 tattgatcgg agtggcggga ggtaccggct caggcaagac ttcgatggcg cgaaatatag
     7861 tacatgattt cgatcctcgc gatgtcgtca ttatcgagca ggattcctac tattatgatc
     7921 tcagtgatat tccgttggaa tcgcgtggtc atcacaattt tgaccacccg gacgcctacg
     7981 atttcccgca gttgcgggct gatatgagtg cgatattaaa aggtcaacag gtcaaaatcc
     8041 cgatttatga ctataaaact catactcgca gtaaaactga atctcaggtt gtaaatggtc
     8101 agaaaattat cgttctggaa ggaattatgg ttttgtatga tccggaattg cgcgatatga
     8161 tggacatcaa agtctatatc gaaaccgcgc ctgatattcg tttcattaga cggctgaggc
     8221 gtgacattca tcaacgcgga cgaacggttg attcagtaat cgcccaatat cttgaaaccg
     8281 tgcgcccgat gcacgaacaa tttgtcgagc cgactaaaca tttcgccgat attatcattc
     8341 cggaaggtgg tcagaactcg gtagccattg atctttttaa aactaaagta agaagccttt
     8401 taaggcacct aaacgaaccg cgataaaaag gagcagaatg agccagccta atctacaccg
     8461 cgtccccaca gacttcgaag aagttgtcgc cattaccgaa aaatcagctg gttggatcga
     8521 ggtcatctgc gggagcatgt tttcgggtaa aactgaagaa cttatccgtc gtctgcgccg
     8581 cgcgcaaatc gcccgccagc gagtggcaat cttcaagccg gtaattgatg tgcgctatag
     8641 cgaagattat attgtctctc ataaccagca gaaaattccg tccattccga tccatactgc
     8701 tcaggaaatt ctccaaaaag ccggcaatgc ccaagtgctt ggaattgacg aagcccaatt
     8761 tttagataat gaattagtcg cggtctgccg aacactcgcc aactccggtc gacgcgtgat
     8821 cgttgctggt ctcgataaag attaccgtgg cgaaccgttc gagccgattc cgcaactgct
     8881 ctgtgaagcc gagtatatta ccaagacgct ggcaatctgt atgaaatgcg gcaacccagc
     8941 caatttcacc cagcgcatca tcaagtccag cgagcgggtt ttgcttggcg caaccgatat
     9001 atacgaagcc cggtgtcgag catgctttga accacctatt atagaagaga aaaaatgagt
     9061 tctctcggtt taacaactat attgactaat taggtgtaaa tatgaaatat ataggcataa
     9121 ttggactact tgtcattctg ggaattgcat ttctgatgtc gaataaccga cggctaattt
     9181 ccttgcgagt cgttatctgg ggactcttac tccaactgct ttttgccgga ataattctgg
     9241 ggaaaatgct gatttcctac tttaccctgt ttatctttct cctattaata ataatattca
     9301 ttcataaaga ccaaattaat acggtcggtc accggtccaa acaatggctt tatatcggtt
     9361 tgataagtat agcgaccgtt ttagccgtgg ccgcttttta ctggtttggg aaaaccggct
     9421 ggttggcaga gttaatcggc ttaattaccg tagcgatcgt tatcaaggct atttttaaag
     9481 ttcgtccttg gcaaaaggaa ttaactgtcg cttggttgac ggctggatta agttggctga
     9541 ttttacacgg ctatactggg caggatattt ttgctttcct ttcacaaaaa gtcgaaaaat
     9601 ttctgggatt ggcgactctc ggcgcagagt ttttattcgg caacctggcg ctcgataaat
     9661 attttttccc gaatgaaacc acctggccgg gattcggaat gcaatttgcc tttacggtgc
     9721 taccggtcat aattttcttc gcttcgttta tggcaattct ctatcatttg ggcgttatgc
     9781 aagccattat caaggcgatg gcgcgtttta tgcaatggac gatgggcacc agcggcgccg
     9841 aaacactgtc ctgcactgcc aatatcttcg tcggtcagac cgaagccccg ttattaatcc
     9901 gcccctatgt tgatcagtta accctttcag aactactgac tattatggtt ggcggatttg
     9961 ccacgattgc tgggggcgta atggctggat atatcaaaat ggggataccg gctggtcatt
    10021 taatcgcggc cagcgttatg tcggctcccg ccgctttggt cattgcgaaa atcatctacc
    10081 ctgagactga ccatccccaa accgccggcg acgtagattt accggaagat accgtcaaat
    10141 cggctaatct tctggatgcg gcggcgcgcg gggtgactga cggactaaaa ttagcggcaa
    10201 cggtcggtgc aatgctgatt gcctttattg ccttaattgg tctagttgat accattttag
    10261 gatttattga ccgtctgatt gacggtaatt tactgaaagg cgcttatacg atttatacca
    10321 actcttccgg attctccccg gtcaaaggtg agttcagcgg cattttccca ggcagtctga
    10381 agtcattatt ttcatttatt ctgcgtccgc tggcttggtt aatgggcgtt tcctggcaat
    10441 acgccggtga ggttggtaac ttgatgggca taaaaatttc cctgaacgaa ttcgtagcct
    10501 attctgctct ttccgaattg attcacacgg gtgctctcga tcagaaatcc attgtgattg
    10561 cgacttatgc cttgtgtggt ttcgccaact tcgcttccat tggcattcaa atcggtggca
    10621 ttagctcttt agcacctggc cgacgttcgg atttatccaa agttgctctg aaagccatgt
    10681 tcggcggggc tttggcatcc tggatgacgg ctacaattgc tggattgtta ttatagttga
    10741 tacattagga aaaatacttt aaaataaatc aatattggat agaggaaatt aaatgaaagt
    10801 aatcttacta gggcctcccg gagtcggtaa gggaactcag gctcagaaac ttcagcagga
    10861 acgtggtctg cgtcagattt caactggcga tattttgcgc caagctatta aaaatcaaac
    10921 ggagctgggc aaacaagccg aagattatat aaagcgcggc gaactggttc ccgacgattt
    10981 gatgcttggc ttgattgccg aaacgctttt cggggatgat gctccgcagg actatatctt
    11041 ggacggattt ccccgtacta ttccccaggc ggaaggctta gaccggcttt tcgcgcaaca
    11101 taacgttgat ctcgacgctg taattttgtt ggaagccgag cccgaagcta ttatcaaacg
    11161 gctttcagct cgacgtacct gccgccaatg caacgcggtc tataatctca ttaccaatcc
    11221 gcctaaaaat cccctgcgat gcgatatctg cggcggtgaa ttatatcaac gagatgatga
    11281 ccgaatcgaa accatccagc gacgtctaaa agtctatact aaacaaaccg aaccgctggc
    11341 agaatactac cgccagcgtg gaaaacttaa aacaatcgcg gctgacggta cgcccgatga
    11401 agtattccaa agaataaatc gtgtaatagg aggataaaaa aacgtggtta ccttaggggt
    11461 taccggagga ctcggctccg gtaaatcaac cgcctgtaaa ttcctggctg agaaaggcgc
    11521 ttatgttatg gacgccgatt taattgccaa acaattaatg gaaaacgatc cccaagtccg
    11581 tgacgaaatc atcgcagctt ttggcaacga tgtttataaa gacggcaagt tagacaccca
    11641 aaaattagct catcgcgcct ttgccagcga agccgatcaa cgcattctaa atgacattgt
    11701 acacccgcgt gtaatagatt attttcagca agaagtggat aagatgcgcg ataagtacga
    11761 cttaatcgta ttagatgcac cccttatttt cgagtccggt ttcgacagtc ggctcgatca
    11821 cactttgcta atctacacca aatacaagta tcgtttggaa cgggctttac gtcgcggtaa
    11881 ccttacccgc gaagatatcc tgcggcggat tgaactgcag atgccggaag aagacaaacg
    11941 cgaactcgcc gaattcgtca tcgaaaacaa cggcaccgaa gaacaactaa aaagcgccat
    12001 cgaaaaactc tacgataagc taaccatctg aaccgatttg aatggtggta agtagtatat
    12061 tttacagttt atttctaatc gctatttata catttttcga tattgaaact tttggggaaa
    12121 aaaccttttt ggggtagtga cagtattagc taaattgttt aatcgaaata aatgagagaa
    12181 tgaacattaa attaaataga ggtaattttc aactcaatca gaactacaga acggtaacta
    12241 atgaagttaa tttgcccgag tcggcaaggt gtgacaggaa aaaagattat tgaattatca
    12301 caaaaataat agccaaagcc gatggcagct ggacaaaggg ggttttttga cggcttcgaa
    12361 aaaccacaat taaaatttga taaaaagggg gataagtcat tatgaaacaa gcaactttta
    12421 gctggtatga cggcttaata tttatactgt ttttcttgtt cgtggtgata ttcagtatgt
    12481 acaaaagccg taagggaaaa actggggagg attactttct ggctggcaga caattgactt
    12541 ggccattgat tggcctttca atgattgcgg ctaatatttc caccgagcaa tttgtcggga
    12601 tggctgggca ggcggctggt tcagcaggtt tggcgatcgc ttcttatgaa tggatggcgg
    12661 ctataacgat ggtgattgtc gccctatttc tcctgcctaa ttttttacgt accggaattt
    12721 atacttttcc ggaatttctg gagtatcgct attctccgct agcgcgccgt ctaatggcgg
    12781 tttacactat tctaatctac gtttttgtgc tggtagcatc ggttatttat accggcggtt
    12841 tgaccatcgt tactttattt tccggaatga aatttttcgg ggtcgaggtg actttgattc
    12901 gagcaatctg gttaatcggc ataattaccg ccatttatac tatttggggc ggtttaaaag
    12961 gaattgcctg ggcggatctc ttctttggat cgagtttgat ttttggcgga tttctaaccc
    13021 tgataatcgg actgagacat gtcggagggc ttagtagctt tttagaggca aatcaggata
    13081 aattgcatat gattctgccg cgagataatc ccgtacttcc ctggacggct ctggttatcg
    13141 gcctgtggtt accaaatttc tactactgga gcgtcaatca atttattacg caacgtactc
    13201 ttgccgctaa aaacctccgt gaagggcaac tgggagttat attcgcctcc tttttgaagt
    13261 taattacgcc attcatcatc gtttttcccg gaattatggc ttggcagtta tttaaagatc
    13321 aaatgaccgg cccgggcggc accgatgcgg cgtttccgac aattgtcagg aatttgattc
    13381 ccaatggatt tctgggcttt atctttgccg ctatcggagg agctgtaatc agttccctgg
    13441 catcaatgct gaattcggct tccactattt tcacgatgga tatttaccag catgccgtta
    13501 aaaaggaact ccctcagcaa aagatcgtcc tggtgggacg gattgccact ttcatttttt
    13561 taattatcgg ttgtttgatt gcacctatcc ttgataatcc gagcctgaaa ggaattttca
    13621 cctaccttca ggaatttcag ggattcattt cgccaggaat tctagccgcc ttcgcttttg
    13681 ggctaatttt taagcgagca cccaaagccg ccggcttggc cgcgctgatt cttaatccta
    13741 taatctatgg tcttcttctg atcttttgcg gttctctacc ggtctttgcc agtgttcaaa
    13801 ttgtcccaat cgcctttcta aaccgcatgg caattacttt tattattatc gttattacaa
    13861 tgggaattat tacctggtta aaaccgttgg accagccggt aaagatgcca gaacgcaagg
    13921 acttcaatat gcaatcttca ccgatcgtaa aatggctcgg gttgacagta attggactgg
    13981 tcatagtgct ttacatcatt ttctggtaaa ttatcttaaa agctgagaat taattgttaa
    14041 atgccagttg acttttccca aattccgcat aggcgcttca atccgctcac cggcgaatgg
    14101 attttagttt caccccatcg tacggaacgt ccgtggcacg gcagtcagga accagtagct
    14161 tcagaaaaaa ggccagagta tgatccggaa tgttacttgt gtcccggcaa tcgccgagct
    14221 agcggaaccg taaatccact ttatacctcg acctatgtct tcactaacga ctttccggct
    14281 ttgtttcccg atacacctca tcgtcgtaga aatgttagga agtatatcag ggatgagagt
    14341 gtatccggca cttgccgagt aatttgcttc tcgccgcggc acgatcttac tttggcggaa
    14401 atggcggttg aggaaatcag ggatgtaatt accgtttggg ctgaacaaac aaaggaactg
    14461 ggtgaaaatt tttcctgggt ccaaatattt gaaaacaaag gtgctatgat gggttgttcc
    14521 aatccccatc cgcatggcca aatttgggcg gtcaatagcc ttcccaacga accttataaa
    14581 gaaaatcgcc gacaatcagc ctactttaag cagaataaat cagtgctatt aactgattat
    14641 ttggactatg aattagaatc tcgggagcgg gtggttctcg agaatgaaaa ctggttggca
    14701 gtagtaccat tttgggctat ctggccgttt gaaattctcc tcctgccgcg gcggcctatt
    14761 ttgcgtttgc cagacctcac tccagccgag agaaatagtc ttgccttaat tctcaaatct
    14821 cttctgacta aatatgataa tctttttcaa tcatcttttc cctatacgat gggctggcac
    14881 ggcgccccga caatgcccgg taacttcgac tactggcaat tgcatgctca cttttacccg
    14941 ccacttttac gttcatcatc ggtgaaaaag tttctggtag gctatgaaat gctggcagaa
    15001 gctcagcgcg atctgacacc ggaagtagct tcccgaacac tcagagaaac aagtgaaatt
    15061 cattacaagt cactaattaa gaacaaataa ggttaaataa tgcccaaaaa tcagataacc
    15121 aactcaccgc aaaaccaaat agataccacc gtatttcaaa aaatctacgg ccataatcca
    15181 agcgtaattg ctgagcaaat caaacgttat acgtatcttg ccgatcggca taatgaaatt
    15241 tttgccgctt ctcctgcgct atttttcagc gcgcccgggc gcaccgaaat tagcggcaat
    15301 cataccgatc acaaccatgg taaagtactg gcggctagtg ttgacctgga ttccattgcg
    15361 gccgtgaacg ctattaacgg ggatcagatt accatttttt ctgagggata tccgcaacca
    15421 ttcatcgttg atatcaatca ttgctcacca gtcgaaactg agcggggcac gaccaccgct
    15481 ctggtacgcg ggatagttgc ccgctttaaa gagcttggtc tgcgggttgg cggcttcaac
    15541 gccacattaa ccagcaatgt gctggtagga tctggcttga gttcatcagc atctatcgaa
    15601 gttctactcg gaacaataat gaattacctt ttcaacgatg gacaaattgc ggctgaggat
    15661 atcgctttga tcggtcagtt tgccgaaaat caatactttg gcaaaccctg cggcttgatg
    15721 gatcaattgg caattgccgt aggcggcgtg gtggcaattg attttaaaaa tccgtccaag
    15781 cctatcattc agaaagttaa ttatgatccc gaacagcaga attacagctg gctaatcgtt
    15841 aatacgggaa ccagccacgt caacctgact gaggaatacg ccgcaatccc tcgcgaaatg
    15901 cgggcagttg cccgagcgtt aggcgggcag gtttgtcgtg atataacaat gcaaaatttg
    15961 attgaggaaa ttccgaattt gcgtcccaag gtcggcgacc gggcaatcct gcgggctttt
    16021 cattttttga ctgaaaacga gcgggttcag caacaaatat ctgctttaga gaagggtgat
    16081 ttcgccgcct ttctggagct ggccaatgaa tccggcactt catcttgccg gtggcttcaa
    16141 aattgttatc ccacctcgaa tcctactgag caaggcgttc ccttggcact ggcgctgact
    16201 cagtatttct taaaaacggt tgggaaaaaa ggcgcttgtc ggattcaggg aggcggtttc
    16261 gccggtacta tcctagtttt tataccgaat gaagttctga gtgattatct gaaattaatc
    16321 acaaaagtct ttaacgaagg cagtgtttct caattgaata tccgcccgat cggcgcggtt
    16381 tgccttaata agatttaaat tttttgaagc gatcagttga gaagtggaca ttggtcaacg
    16441 gttattagtt gttgggcatt ggtgaggtag gtagggcaga agttaactat tcgatattat
    16501 atcaccccta attcatattg tcaaacgtaa atattcgtcc gagatttttt ttataaatta
    16561 tggtaaattc tttcataaaa tctttatcac caactccgct atattattaa attccagtcg
    16621 ctgtgttgta ttttaaggtt agttatcaca gattattaat cttctcggta ggtctattaa
    16681 tcgcgataat ttgccgggca atggctatgt ctgataccga ttctgatgtt aaaaatttaa
    16741 caattctcag cgacaccaat ccgagattac tcacagacgg cacactttac accgacttac
    16801 aactttctcc tccggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaat
    16921 ccaactggac cgaggctact ttccgtttcg acgacgggat taatcaaccc cctctaccac
    16981 cggttgaggg ttttaatccg gatggtatca tcgtcgcgag tcattatccg gaattgaaat
    17041 ggtctccagc tattgatcct gatgtcgccg atcgtttacg ctatgaaatt gtattaagtc
    17101 gcgatcgctt gttcggcagc cgaacctata ttttactaaa aagcacttat gaccagaatc
    17161 aaatagtggt tcaaactcct ctgctggaga attgtcaata tttctggcgt gtgcgttcca
    17221 tcgatctggc tgaggcacgc tccgtctggt ccaaatccaa taccttttgg gtcaatcaga
    17281 ttaatgaagc tcctcagcgg ccagtacgaa ttatccaccc gcataatctc gaggaaatca
    17341 caccaaattc gtatttctgg tggttaccct ccgatgatcc tgatcccggc gatcagttaa
    17401 attatcaaat tgaatgtagt gaatctctta acttcactga acccatattt tcctaccgga
    17461 ttcccagaac tattactgcg aaggaggtag aaactaaact tgaactgcca caaagggcta
    17521 ttggagtctg tcttgatgaa atctcggcga tcagcctttt gaaagataat acgctgtatt
    17581 actggcgcat tcgggctttt gatcaatccg aaattagtgg aacatcagcc agtcgtccgg
    17641 tcagagtggc atttaattat caaaatgatc cacctctgcc ggttgttaaa ggtttctatc
    17701 ctgctgatga cgttatcatc aaaaccctgc gcccggaaat ccgctgggca aatagtagtg
    17761 atcctgattt tagtgatttt caagccagtc taacttacca gttagaaatc agcagagacc
    17821 cccagttccc cgaaaatgca tgttcggtct atacaactga accggggcag actgttttta
    17881 ctcttcctga aaatttaata gaaaacgaac gctggtttta tcgtgttcga gcttgtgatc
    17941 aacacggcag ttactcgctc tggtcgccta ccaactcctt cattactaat gccataagtg
    18001 aggcgcccta tcctgttacg gatggttttt taccgaaaga ttcagcggtg gtggaaactg
    18061 ctcagccgct aattagctgg ttgccgtccg atgatcccga cccggatcaa gatgaacgcg
    18121 atttatatta ccttgttcgt ttctacgagg ctgataatcc caaaaaggtt agtcaggttg
    18181 ccagcaaaac cggcataacc agtctccacc tcccagaatt aaaagaggat aaatattatt
    18241 tttaccaaat atcagcggtt gatccagacg gcaatcgcag tgaatggagt cagctggtat
    18301 gttttggagt aaatgctgtt gatctaccac ctggcccatt tgccttactc gcaccattta
    18361 acgggcagga ttcggttgcg ctggatgccg gattcgtctg gcatatcgcc cacgacaaag
    18421 atccaggctc gcagatcaat tatacattat actacggaac cgatagcctt tttttgacga
    18481 acttccgtga aattatgttt gagcaaccgg aagaagatac aattatggtt tataatccac
    18541 ccgaacgtct taactacgcc actcgctatt tctggaaagt tgtggctact gacaaccgcg
    18601 gcaatcaatg ctgggcttct cagactaacc aacgcccgtt cgttttcaca accgttggta
    18661 cccaacgctc gatcgccagt atatttaaga atttccgctt acatcagaat tatcccaatc
    18721 cctttaacat agagactttg attagatatg atgttccatt ttatggacct gttgatgtat
    18781 caatttacga tttaatcggg atgaaggtca ctacgctagc taccggtaat catgcggccg
    18841 gcagttacag tgtttattgg aatggtaccg accagaacgg tacgctggtc gctaacggtg
    18901 tttatatctg ccgcttgacg gcgcccggct atatgtccca tatcaaagtc gtacttatgc
    18961 gctaatttat agagattatt taatctcttt gctaccatat tacatcctgg ttttagtcat
    19021 aatcttggct tgttttaggt taagtttgga atatagccgt tttgactaat gatcaatgac
    19081 cagccccacc ctaacgcaga tagtctgatg caacttgtgt taagggtatt gggcttttca
    19141 ttcctaacct tagcctcgtt caatcttggt ctattttact aaaatatctg tgtgccttag
    19201 agcctttgtt gttaaaggag cctttttgca cccaaatcct agaaggcaat aaatgctatt
    19261 ttttatcagt caatcgggat ttcatttcgt caactaattc ttgcacttcc gccacacgat
    19321 tgcggatttt ttgaataagt tcggcttctc gttctttggg attgccattt tcagattcgg
    19381 tcgtcactaa attaattctc caaaatattt aaccgtaaat aattaccatt ctgtaattac
    19441 ataaggaatt ttaactattt gccaattcac gagtaataat ttaaaaatcc acaactaaaa
    19501 tgccgccatt tttttgtagc taaaagaagt aatacaaaat taatcgaann nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc gcttcttgtc atcctgagct tgtcgaagga
    19681 tgacatttcc atctccctat ttcaatttct cagcgtcagc cttagccttt tcattactta
    19741 atcccataag ggcgaactat ctggagaatc tttccagcat tagacattga accagtaaga
    19801 agccttaatc ataaaaatgt tatccggctt aatttttccg atgcgattga ccgaacggct
    19861 taggtcaaaa tctgcctcac tactattttc aaagcgacca tgcgtccaga ctaagtaaat
    19921 cgccgaaccg agcgcaaatt cccagcgcaa taccgcgctg gagcggaaac tatatacatt
    19981 aaagtcagga ttgtcaaatg aaaaggagcc ggccggaccg ttaccgtccg gatcaacagt
    20041 gtaattccca tcgctatttc tattaatttc cgtaccttct tcataagtaa aaaactgata
    20101 ggttttcggt cgggcaaact ctttaaaatc gtaatattcc gccgcaccat aaagcggttg
    20161 ggcatataat tgaagtgtca aacgcggggt aaaagtccag tccaagcgca ggctggcaga
    20221 tatagtcttt tgatccatct tagcaaagac gtagcgttta ccataagttg cgtccgcata
    20281 ggggtcatcg aaaacatcca catattgggc ataattatga ttaatagaaa tgctgggaga
    20341 caactcaaag gctaaattat cagaaacttt ccaatcgagc ggaatttgta aataatgact
    20401 gaccgaggcg gtgtcattgc cgctggtttc gtagtagagc ctgcctacca acggtttgcg
    20461 ttcatctgtt gatatttgaa tatatcccca ccttccggat ggagatatgg ctattggacc
    20521 gccgcgggta agtctggtat cataagtttc ccaaaaatag ccgcttccat aatctatcca
    20581 gtaataattc gagaattgaa tataacccaa attcataata ccattgttag tcaagttacc
    20641 tccgcagtcc ataccgagtg tgtgagccag aattaaatta aatttgcgat agaagtgatt
    20701 cggctccgtc cactgataac cggcaccgac gtgataattg ataacatcgc ctctcgataa
    20761 atatcccagg tcactaacct cgaaccgtgg gtcaattaaa ccaaatgcgg cattaaaaat
    20821 gacattgcca cgctgtttat tgagattaat ccgagtcgca aatccagtca gcgcggtggc
    20881 attactatct actcctaaat gctcagcatc cggtcgttga aaataatgac gcgcgttcgt
    20941 ctgaacgtcg gtaatacaat tctcattacc ctgaatattg gacatcgcgc cccatccggc
    21001 aattacccag gttttattag tatccagaaa agtccagccg tccagcccca ctaccagggc
    21061 atccgaattg agatagtcgg cgagactttc atcccgaaaa aagcgtttgg tagaagtggt
    21121 aattattccc agcccgtggc gaccctcttg aaattctttc tgcgtccgca gaatcgcata
    21181 attagtcagc ggctcagctt cgattttcga gtcgagaccg ttgggactaa cgcgggcgaa
    21241 ctcacgcgct gtcagggcgt tcaatagccc cacattccag ttatcgccaa ctttgcccgt
    21301 caattttccg gcggagatga tgcgtgttcc gtctggcaca tcggtataat cgtaatccga
    21361 caggtcgcgc tggggggcgc gtccgatgcg ccggctataa aatatgctgg gcgtcgaaaa
    21421 attcatattc caataattat tggaaccacc ataaccgaaa ttaaaaatat ccgatccttc
    21481 gataaagaac gggcgctttt cattataata ggtttcgacg tcggacagat taatcactgc
    21541 tggatcaact tccacttgac caaaatcagg gttaatggta ccgtcaacgg tcagattatt
    21601 acccacccca atcttaaaat cggcaccggt actggcaaaa tagcgtgaac cgtcgttaaa
    21661 aggatcttcc ggttcaactg ccaaaaattc gcccttgcct ctgatatatg gcaaaacctc
    21721 gattttgcgt tccgcattaa tgccctcgat gcccaccagc tcgatgaaac gcgaaaccat
    21781 tcctgattca ttgcggggcg taaaaaagct catatcctcc tcattcttac gttgaatttt
    21841 acgatacaac gccactcccc acacatattg atcgcttttc tggaaacgca attgtgaaaa
    21901 agggatgcgt atttcagccg tccaaccttc atgattacgt tgtgcggcgc cttcccaaac
    21961 accgtcccag gtatcatctt cccaattatc attataaaat tttccgtcgt aaatcgctcc
    22021 tgcggctgaa accccaaaaa aataggcggt gcgtttgtca ttgaaaggac tcaaggcgat
    22081 cagaaagaga tcggtgttcc agtcggaatc gcgtcggcca agccgcgcta caattgaatc
    22141 tggggctgaa tcataaagcc gggcgccgac gtagagcgcc tcgtcatcat agacaatcca
    22201 gatatccgat ggctggcttg gttgagcacc ttcattcggt tcatcttgaa ttaaaggtgg
    22261 cgccggaata gctctttgcc agatttcctc acttaaaatt ccgtcaatac gaatagattc
    22321 gtccgttcga gtcgccttta cctgagtatt gcccattgtt attccggaca aacatattgt
    22381 cagaaagcac agattagcga caaccttaaa ctttttcact attaaactcc cggttaatca
    22441 ttaaaaaaac acaacaacgc cctatagatt ggattaacca ggatttagtt gtcctatccc
    22501 tgagttattt tcaacccagt agacaataat ttgtgattag aatcaaaagt cggttgaaag
    22561 ttctcagccg acatgctcct ttaaaatgct actgagatct tccatgcgat atggtttagc
    22621 gattatatca ataaatccat atttttgagg aacagtcatc atcgaatcgt ttatataacc
    22681 actagagact aaagctttaa catcaggatc aaatttcttt agttctgcga tggtctcttt
    22741 accacccata cctccgacaa tagtcaaatc cataatgacc agatcaaatg gttgcccggc
    22801 ttccttggct tccttataag atttgatggc ttcttcaccg ttcggaacgc atttgacatc
    22861 gtagcccata ctggaaatca tctcctcaac cacttcacaa atcaattcat catcatccat
    22921 cactaaaatt cgaccattcc cttttgaaat ttggggttcg gctttttgag aacccttttg
    22981 aacagcggcg gttgccggca gataaaccgt aaaagttgtg cccttgcctg cttcagaatc
    23041 cacaaaaata tatgcatcgt gttttttcag tatagaataa acaaccgaca agcccaaacc
    23101 gtttccgtga ggtttagtgg taaagtaagg atcaaatact ttggataaaa ggtttttagg
    23161 aataccaacc ccagtatctt gtatcgcaat tttcaagtaa tacccggatt taattggtac
    23221 aagaccatct gtttttattt cctcagcatt agccgaaatt tttaggttgc ctccggacgg
    23281 catagcctga atagcattta taaccaaatt ttgaatcact tggctcagct ggccacgatc
    23341 agcctccaca ttgggcaaat cgggagcaat gtcaaactgg cagttaaccg acgatcccac
    23401 catagcaaat tcaaccaatt ctctcagaaa actctcaacc tggatggatt ccataaccgg
    23461 cgcaccgccc ttggcaaaag ttaacaattg ctgagtcagg gagcgggaac gtaaagcggc
    23521 cttctcggat ctttcgagga tccgagttat atcactgtct ttaactttca tctttgccag
    23581 tgatatgtta cccaaaatac cggaaagcat gttattaaaa tcatgggcaa tgccaccagc
    23641 caggatactc agagattcca acttctgcac cttgatctgc tcgtcttcga caattttttg
    23701 aatagttaca ttgcgcattg taccgatata tcctgtaaat ttcttaccgc catcataatt
    23761 gggtacgcca tttacttcaa ggataatttc ttgaccgagg tgattaacta tagttaactc
    23821 caggttcttg atcggcttac gcttggtaaa atgtgacgca atgattttgc cacctttttc
    23881 ttcagaacct ttaggtagaa tatcagcaaa ctgtttacca ataagtttat caggggtacg
    23941 cccgagaata tcttttatat tcgggctgac atataagacc cgtccggaat cgtcagcttg
    24001 ccagataaca tccagactgg attctactaa atgacgaaat ttagcctcgc tcatccgaag
    24061 cgcctcttcg gtgcgtttaa gctcactaat gtcggacata gtggtctgaa tggaacgaat
    24121 acgaccatca tcgtccctga tgatataatc agaaatagag aaagtcttta cttctccact
    24181 cttagttaaa taatgacgtt cattccccgt agaactcgtc ttcttgctta atttcttttc
    24241 atcaaaggat ttacgagccg attctctttc actttcgata acgaaatcaa agatcggtcg
    24301 cccacaaacc tcctctttgg agtaacccat aatttcacac cattgacggt tgacatccac
    24361 aataatacca tcaggagtaa tggtgtggta agctattggt gcatattcat ataaccagcg
    24421 aaatcggcga gcagattcag ccagctcggc ttcggcattt ttacgcttgg taatatcacg
    24481 aaaaatgctc agtaaataaa cttgctcacc gggaatatcc agaaaagaat tggaaatttc
    24541 aaaccagact ttctgaccat tccagagaat tgattcgcat tctgttaagg atttaatatg
    24601 tcgtcgagag aaattatttc ggtaatttgt ttcaatctcc tggcgctctt ccggcggata
    24661 aataatccct atactggcat tttccagctc gtttcggcgc atgcctacct ggcgacaaaa
    24721 ggctttgtta acggcgatta ttttgccgtc cttgtccgtc aaccgcatgc catcaaaact
    24781 gccttcccag attgattgga aacgcgtttc gtaactttgc agggcaatat tggctttaac
    24841 cttttcactt atatccgtta taaaaccttc gagcactacg agattttcgt ctccgttata
    24901 gataccgaca ccttgctcac aaacccactt ttcctgatta tcggctgtta taatccggta
    24961 tagtaactga tagggactcc gctttttcag agaagcacca atttcatcaa atacatattg
    25021 ccgatcattc gggtgaataa tctggctata ggttatttta tggtccatta aaaaatcatc
    25081 aggcgaataa ccggttaatg aacggcaacc atcgctgatg aaaatcattg tcctttcggc
    25141 gtcatataaa caacgataag ccgcacctgg taaatttctt accaaagaag ccaatttatc
    25201 ttccgattca caaacaagtt cttctgctat atcttcataa ggacgtttct tcgtattcat
    25261 ttaaacccct caaatttatc agaacagcta ctattcgaaa aaaaccggcc tacacaatgt
    25321 cgcccaagaa ctaaatattg ttttattgtg tgccgttttc cacatgtgaa tactaaaaaa
    25381 taatatttaa aaatgcaagt cgttttttag gttgagggta aggcaaaggc taataagagg
    25441 acagaaacag aaaccacacc tagctgtaac cgaatatcta ttaaacattt ttgattaatt
    25501 tgaaagcgca atatgttgta taacactttg taagttaaaa tttctgaaaa atagccgtat
    25561 tatagtgtta aaaataaatt tctatcaatg gtcaaaaaag cacattggag atccaatgta
    25621 ttcataagcc agtctaaaga tagatgacta tatttgggga taaaaaatta atgattttct
    25681 gcggttatca gacaatcatg ttctatcctt ctgaatcggc tgaacttccc gaccaaaagg
    25741 agttaatgcc agtgatagca atttgatatg ctgtcttgca aagggaatac cgataatagt
    25801 gatagcacac aatgcggcga aaactaaatg cactaccgcc agctctaaac caccaaaaag
    25861 taaccagata atattcatta cggtagacaa acagcccggt tcagatgctt tatagactat
    25921 cttttgtcca aacggcatta gtcccaacac cgaaagcttg atacattgca taccgaaagg
    25981 aataccgatg atggtcagac atagcaccac accgccaaat aaataaaaga aaaaaattag
    26041 gattccgcca cctagaaaaa tccacaggat attgcctaat attgtcatac ttactcccat
    26101 ttaaagacta actttaatac aaagaaacag gttttacgat tggacatagt taagtatcat
    26161 tgcggctaat tgccctaacg tattccacca taccttaaaa agcgatctcc tggcttaagc
    26221 taacactcaa tccaacaaca ccttgccagt acgatgaaaa tcaaacaata cttctctaaa
    26281 aacgataaaa gtaaccaact catccatatt aaatacaata tgcagatagc gcatatagtc
    26341 tggcacctcc agtccggtgc tttcgccgcc ggcataggtt ccatcaatat tgaattgttg
    26401 cagaaatttt tcgtcgcgta ttacagctgt cgggaaataa tggaccgcga tttgcctttc
    26461 cggatcaatg tataaaaacg gcactggaat aaaggtcttc tgctggcaat tttcacagac
    26521 caacaggttt attttccctt caaataattc ctttttcact tctttgtcaa gagctgtatt
    26581 gagcgattgc caaattttta cactttgttc gtgctggcaa tgggggcaaa cgacgatttt
    26641 ggtttcatta attgacattg gattttcctt ttagaattaa attattcgcg aattccaagt
    26701 ttagcgaaaa taaaggcata tttaaaggct acttccttta ggtaatcata gcgcccggat
    26761 gctgaaaaat gtcctttgcc catatcggtt ttaagaagca aaacgttatc atctgtcttc
    26821 atcgcccgca gtttggcagt ccacttggcg ggctcccaat aagctacgcg cggatcattc
    26881 aatccggctg tgattagcat atgcggatag gctttaggtc caacgttatc gtacggcgaa
    26941 taggatttca tataattgta atacttttcc agatggggat tgccccattc ctcccattca
    27001 atcaccgtca gcggaatcga acgatccagc atcgtattga tcaaatccac aaacggtacc
    27061 tcagctacta cgacctcaaa taattccgga cgcatattga ctaccgcacc catcaacaag
    27121 ccgccggcgc ttccaccatt aattaccaac ttttcggctg aggtatattt ttcggcaatc
    27181 aacatctcgg cgcaggcgat gaaatcggtg aaagtattac gctttcttaa aagtttacca
    27241 tcttcatacc actgccgtcc cagttctccg ccaccgcgga cgtgagcaat tgcataaata
    27301 aatccgcgat ccagcagact tagtcggatt gaagaaaaac gcgcttcgct gggaatccca
    27361 taagcaccat aaccgtaaag caaaaatgga tttgaaccgt cttttttcat acctttttta
    27421 taaaccagag aaatcggaat tcgaactccg tctttggcaa cagcataaat tctttccgat
    27481 tgatactgcg tctgatcgta gccgcctagc acctcatctt gctttttcaa ttcacgagtc
    27541 tgactttcca tatcataatc ataaactgaa tttggcgtaa ctaaagacgt ataacggaat
    27601 cgcaatattt tcgtattgta atcaggatta ttatcactgt caatggtata gaccggttcc
    27661 ggaaaatcta tataatgggt tgcaccggta tttaaatttt cgactcgaat ttgttccaga
    27721 ccgttttctc gttcatatac aaccagatgg tcgcagaaaa gatcaatgcc agtcagcata
    27781 acattgggac gatgaggaat tttctcctgc caattgtgtt tttcgggtct ggcaattgga
    27841 gtttccagca aacgaaaatt tatggcattt tcattagtta aaatgtaaaa cttgtcgcta
    27901 tgatgatcaa tgtaatattc tacgccggtc ttacgcgggc agaataatcg aaatcgtcca
    27961 tctggctggt cagccgatag gtaatgaact tcggttgtaa tctgacttcc caaggttaga
    28021 atgaggtaac cgtgactgcg cgtcttagct attccgagat aaaacttctc atctggttcg
    28081 tgaaaaacca taacatcatt ttccggcgac tggctgagct gatgacgcca gagcttgtcc
    28141 ggccgcagaa tcgtatcgag ggtcgtataa aaaacagtac ggttatcgtt tgcccattcc
    28201 agtccatagg aaacatcaag aattgtatcc ggcaacatct ggccattgat taggtcttta
    28261 aaaaccaaca tatgcttttc tgaaccattg aagtcaacgg cgaatgccag caaactatga
    28321 tcgggactta ctttgaactc tccgattgaa cagtatttct gcccgctggc tagccggttc
    28381 tgatcgagta ggatttcctc attcgcatcc agcgaacctt tcttgcggca ataaagcgga
    28441 tactgattgc cctttaccat gcggttgtaa tagtaaaagc tatctatttt gaccggcacg
    28501 gactcatccg tttccttaat ccgggctaac atttccttgt aaagcttttt ctggaagcgt
    28561 ctggtcggtt tcatgattgc ctcagtgtag gcattttcat cttcgagata ttggatgact
    28621 tctggattag atttttccct tagccaggca taattatcaa ttagtgtatc actatgaata
    28681 atcgttgttt tgggttcgat ttttgccgtc ggcggagtca gcggtcgtga gggacagccg
    28741 cttagccaga ttgccagggc gaggctaata gctgatatac tcgttcttaa ttgtttccta
    28801 aaattcatat tctctcctga ttacttattg gttcgctttg ccaattcggt ggtttcaaat
    28861 aaaatataat ccgtcttgga catacccatt ttgcgatagg ttttctgagc aatccggtta
    28921 tcgcgctcga cgtaaagccg caaaccgcag acattaccga ctttgagggc ggcattctga
    28981 acatagtcgt acattttgcg gtaaattccg cggcgccgga attccggagg cacatacaca
    29041 ctctgaatcc accagaaaag cccattacgc caatcgctcc attcataggt tatcattaga
    29101 cagccaacca tccgtccgtc actctccgcc acgatataaa aaccccattc cgggtgctgg
    29161 aatagattac gcactcctgc agagattact tccgcactta aaatacggtt ttcagtctct
    29221 tgcgccatcg cccgattaaa agcaaccaag tcgtccgcat cggatagacg ggcggaccga
    29281 atcagaattt ccgcgttatt ttgtgccatc tatttttcgc catttgacgg aattaatact
    29341 aaacattatt tggatgatat gaaatagttt tcgtgttggc agaaaatttt tatctcagga
    29401 cggatagaga attcaattcc aaaatgggaa tcattaacta aagatacaac
//
LOCUS       DAOMJU010000017        29254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1422_length_29254_cov_18.179869, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000017 DAOMJU010000000
VERSION     DAOMJU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 29254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29254
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1422_length_29254_cov_18.179869"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PLO58_02915"
     CDS             complement(<1..1189)
                     /locus_tag="PLO58_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73772.1"
                     /translation="MKKILQRFFLICPKTGKVRGIKPLSGWSKLLFPITGLLALIWVV
                     VRVLPKPTRATYPCMRVAMPLASGFIVYLIGVIASATAFMKAKKMLAKSRYLLATGFV
                     ILGLLASLLIFHNSRETAYATYATIEHEANIPMGTAKGIFPGRVVWVYNPDATNENCV
                     PDSTVSSWWGSGYTTYRAGKAWFLPQNNNQAVVDSMVSSAIQSLTGATSDSAAWVAIF
                     KYHNTTREKGAVDYSPGEKIFIKINATSGWGGNYSTTDLSCAKNSSYGISETSPAVVM
                     TVLRHLVNVVGVAESDIYIGDPMKHIYKHLYDYWHSEFPAVHYLDHDNPDSLGREHAQ
                     VGTKTKIYYSDKGTILRENVWDANRPGEGPVYDDNVYAILEDAEYLINIPMLKGHRRG
                     GVTM"
     gene            complement(1262..3655)
                     /locus_tag="PLO58_02920"
     CDS             complement(1262..3655)
                     /locus_tag="PLO58_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetylchitobiose phosphorylase"
                     /protein_id="HQO73773.1"
                     /translation="MQFGHFDDKNKEYVIDRPDTPRSWSNYLGSTEYGAIITNNAGGY
                     SFFQSAAQGRFMRLRFNAIPMDQPGRYFYIHDLDKHDYWSTSWQPVGKPLDKFKTVCR
                     HGTAYTIIESEYDNILTETTYFVPLNRHFECWLLKIHNRDNVTRRLRLFTFIEYTNNW
                     HVWQDTFNLQYTQFILRMNVVGNIIDHGINVNLADDTGDLWLHDGNKHTFIGVVGAEV
                     SGFDTDREIFLGGYRSYHNPIVVEKGECLNSIAVGDNGVGVLQIDLELKPGESRELAV
                     LMGIGRAEAEGRKVLSEFQNLDNIHIEFEKLKQHWHQRLQGFLVQTPDSEFDSMTNVW
                     SPFNCLITYAWSRAASLIYSGERDGLGYRDTVQDLLGVLPLIPEEAGERLELMLTGQV
                     STGGAMSVVKPFDHHPGREIPPKEEEYRSDDCMWLFNTVPAYVKETGDLSFFDKVLPF
                     ADEGEATVLGHLRRAIEFNLERTGTHGLPCGLYADWNDCLELGQRGETIFVAFQLRYA
                     LKTYIEICELLERLSEKKWAVEQLQKLDENLRKYAWDGQWFLRAYRYDGLKYGSHTDE
                     EGSIWLNPQSWAVISGFADKDEAETAMQAVYERLATEHGLMILAPPYIKTDHRIVKAP
                     LFNAGMKENGSIFSHTQGWAIIAETMLGHGDRAYQYLRAYLPAAYNTRAEIRQIEPYV
                     YCQFTNSRYSPRAGASRLPWLTGAAAWSYYTATQYILGIQPDYFGLRIDPCLPSNWKE
                     IQITRRFRNKMFNILIKNPNGTQKGIRKLIINNQDLTGNLIPLDIIQNENKVVVEMQ"
     gene            complement(3680..5137)
                     /locus_tag="PLO58_02925"
     CDS             complement(3680..5137)
                     /locus_tag="PLO58_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO73774.1"
                     /translation="MAQEITANNSTTQVRDRLPVLTKICYGLGTGLDMWGFWLYPGVA
                     FAVFNMYLGVNPMLVGLALTLIRIYDAFTDPIAGWISDNFRSKHGRRRPFILGAGVIS
                     GLGLPLLFLVSPAWADQTFLGLSVVFWYMLLSNLIYIPIISLFTVPYNSLGNELTPDY
                     EERTSVMTYRSVMQKIFEVGNFYALRFTNLAWFLIPGTGRKNTLRGMQVYTSIIGVLM
                     ALFAIIIFFRVKERYYEKVVVKTKERIKLASSFYETLKCQPFRMMLLLGGSFTLGTSM
                     VGTLGYYTTVHYVCGGNNIVGDNWNFWMGIAFMIGGLLGGPILNRVAYYLEKRKAVMV
                     AAIIGIIGYGGSWFLYTPLVPWLQTIASGLMGLSSAGLWMLHGSIGADIIDYDELNTG
                     KRREGSFTGCSSYILKLGNSLGGFLAGSVLNFAHFDSSLAVQAPETIFWIRAMLACIP
                     VVGLILVIFFITRVNLTRQKCAEIRSALEARRGTV"
     gene            complement(5185..6174)
                     /locus_tag="PLO58_02930"
     CDS             complement(5185..6174)
                     /locus_tag="PLO58_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HQO73775.1"
                     /translation="MKYRIVGNSIPNLPWEERPEGCSDIVWRFSKNPLIGWNPIPKAA
                     RIFNSAVISYKSEFVGVFRADQKNGRATLFSGKSHDGLRWEIGPDPINWVDEQGNPKP
                     LSYGYDPRLVKIDDIYYIVWCDDLHGASIGLGKTQDFKTFTRMPNPLMPFNRNGVLFP
                     RKVNGKYLLLSRPSDSGHTPFGDIFLSESPDLIYWGNHKFVMGKGGMGWWQGTKIGAG
                     PIPIETSEGWLLFYHGVSTTCNGFVYSFGAVILDIDEPSKVLYRTRDYLLTPEKPYET
                     TGFVPNVVFPCANLCDTATGRIAIYYGAADTYTALAFAQTDELIDYIKNNSEV"
     gene            complement(6203..7612)
                     /locus_tag="PLO58_02935"
     CDS             complement(6203..7612)
                     /locus_tag="PLO58_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO73776.1"
                     /translation="MAKYLTLREKIAYGFGDLASVLYWQTFMLYFTYFYTDVFLIPAG
                     VAATMFLVSRVWDAVNDPLMGIIADRTNTRWGKFRPYLLWFCVPLAVAGVLTFSVPNF
                     GMTGKIVYAYVTFILIMMLYTAINIPYTALLGVISPDSDERTSVSSIKFLFAFTAGVI
                     VSATLLPMVKALGKGNEARGWQLSFVIYGIAAIIFFLIAFKGTRERVHPPKSQKSSIK
                     KDLYELVTNSPWLILLFTTITFILFVAIRSSVTVHYFKYIVGTREVSLPFFKNKTWDF
                     ETLTSVFNTVGQISSFIGVLLVSWFAKKVGKKPAFMIIFVIAIFSTSIYYFLSPEQIG
                     LIFLFQIIGSLTGGPLSVLIWAMYADSADYAEWKNGRRATGLVFSASTMSQKFGWAFG
                     AFVALNLMSRLGFMPNQAQTPQSLRGLLLLFSLIPAAFGILALIIVSFYPLNNQRMAV
                     IGADLKERRAQSGETIAPA"
     gene            complement(7715..10153)
                     /locus_tag="PLO58_02940"
     CDS             complement(7715..10153)
                     /locus_tag="PLO58_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HQO73777.1"
                     /translation="MKFGHFNDQDREYIITTPETPYPWINYLGSQDFFSIISHTGGGY
                     SFHRDARLRRITRYRYNNSTLDDNGKFFYINDNGDVWNPGWKPVKKALDFYECRHGLG
                     YTRISGQRNDILAEVLAFVPMNFDGEVQQVTLKNTGTSVKEIKLFSYVEFCLWNAWED
                     MTNFQRTFSIGRAEAIGSTILHFTDYKDRRNHFAFYSVNAPIDGFDCDKESFMGLHNG
                     PHEPVAVFEGKNRNSVAFGWSPVAVHQIEVKLNPGESKSFNFILGYVEYPETEKFTAD
                     GKINLTKVRQMQSRFESPESVTKAMNDLREFWDGLLSVFQVQTGEEKIDRIVNIWNQY
                     QCMVTFNMSRSASYFESGIGRGMGFRDSSQDLLGFVHQIPERARERLLDIAATQLPNG
                     GAYHQYQPLSKRGNYEVGDNFNDDPLWLICGVVAYLRETGDFAILDESVPFDNDPNNQ
                     ASLFEHLKRSFYHVVNNLGPHKLPLIGRADWNDCLNLNIFNTDPDQSFQTGPLKTDGK
                     TAESIMIAGAFVLYGQEFVELCEFLGKMDEATEARQHIDEMKKSIDRFGWDGEWFLRA
                     YDAFGRKVGSQENEEGKIFIESQGFCCMAEIGKEDGRMQKALDSVKKYLDCEFGIVLQ
                     NPAYTKYYIELGEISTYIPGYKENAGIFCHNNPWIMIAETKLGNGEQAFEYWRKICPS
                     FLEDISDLHRTEPYVYAQMIAGKDAYKPGEAKNSWLTGTAAWNFLAVSQYILGIRPQF
                     DGLLIDPCIPSDWKSYTVRRLFRGKNLNIEVRNPAGVQKGVRRITLNREALSNNLIPC
                     SKLKNTNEVIVDMG"
     gene            complement(10220..12355)
                     /locus_tag="PLO58_02945"
     CDS             complement(10220..12355)
                     /locus_tag="PLO58_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HQO73778.1"
                     /translation="MRKTYLGGFLFFLILLNLFATNNRLFSQEFNRDMETFQPLAYAE
                     ADKLAESILAQMTLDEKIALIGGDKYFFIRALPRLNLSEIYMSDATQGIHIRQSFNEI
                     DLSDYQPEKSTAFPCPISLSATWNRELAYQYARAIGEECKAANIGILLGPGMNIYRQS
                     QCGRNFEYFGEDPYLAARMIENYVQGVQSTGTVATLKHFLANNTEYYRKKSNSVVDER
                     TLHEIYMPAFKAGIEAGAKAVMTSYNLLNGEWCGESAYVINYLLRNQLGFQWLVMTDW
                     WSVYNGEKLAKSGQNLEMPFTIALENVHQLLEDNKVHVSDIDRMAQSILRTCFAMKLY
                     GRSRTAVEDSFYEAHEKVALQTAREGIVLLKNENNILPVKEQVKNILVTGDYVKKLAV
                     GGGSAYVEGYNNRQMISELQKVFGDRIKYVKNPTVKQIKSAQLVLCNVGTSDSEGWDR
                     PFELPEDQEQKVLKCVNNNPNTVVIVTSGSGIRMTGWNEKARAIIYAWYPGQIGNQAL
                     AEILIGKVNPSGKLPITIEKEFKDSPGYGYIPEGESLYFDWNGEGERAHPVYDVRYKE
                     GIFIGYRWYESQKIEPLYPFGHGLSFTSFSYSDLQVTPGKFSEVDTVSVTFSVKNVGK
                     CQGAEIVQLYVSDLESSLPRPAKELKGFQKIVLEPGEIKTVTLKLSQKDFAFWSPAIK
                     NWTTEKGKFVILIGSSSQDIRLSKEIELL"
     gene            complement(12399..13757)
                     /locus_tag="PLO58_02950"
     CDS             complement(12399..13757)
                     /locus_tag="PLO58_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HQO73779.1"
                     /translation="MNSVQRHNRHKCKIIKVFWLIVILQIPIQCEQNNKSDQIGGSEL
                     TFVEEHGNLTVSGNRIIDQNGDPIVLRGMSLFWSQWMGKYYNYNCIKWLRDDWKCTVV
                     RAAMGIESGGYLNNPASEKAKIVTVIEACIDLGIYVIVDWHDHNAHEHQAQAIAFFQE
                     IATDYGAYPNVIYEVFNEPEQDNWKTVLKPYLNAVVDSIRAIDPDNLIVVGTPNWSQD
                     VDIASQNPLSQNNIAYALHFYAAYQYHKQTLRNKASTALNNGIALFVTEFGTVMNTGD
                     GPIDTAETRLWMNFMESNQISWCNWSIADKNETSAALKPGASANGGWSASEITESGLL
                     IRDYIIRGNTKQPNLVRKTRLIPAGFALAQNYPNPFNSSTQISFELPVSAGVNLGVYD
                     LRGNLICTLLNKVFPAGRYQTTWETNGAIDEIAGGVYILRFLADSEIGTTQISRKLSH
                     IK"
     gene            complement(13865..15601)
                     /locus_tag="PLO58_02955"
     CDS             complement(13865..15601)
                     /locus_tag="PLO58_02955"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HQO73780.1"
                     /translation="MRFKTITSSLIVCIAITTVLTAQNLIGPNWKFQPGDDLAWADPQ
                     YDDASWNSIIAGRTWEVQGYENLDGFAWYRYSVIIPSTLKEKAVKHGGFILRLGRIDD
                     SDATYFNGELIGQMGGFPPNYIGAYGLDREYIIPVELVRWDQPNVIAIRVYDAGGGGG
                     LYYSAAELNVIGLSDQLKIEIALPQKDHVIKNCSEIVLPVIMRNESNESIEGILRLAI
                     NSDFGDKIYDKELKITMKNNSAKKYSYNLKKLQPGFYLGAVSFESLGWNKLHKFAIAV
                     DPEKVISPVDAQPDFDAFWEQAKAELATVDPQFKMIRQDELCTAERDYYLVEMRSLGN
                     ILIRGWYSVPKNPGKYPAFLQVQGYGTNMQPTNLITEDGFISFGLNIRGHGNSRDEID
                     PGFPGYLQYHLENKYEYIYRGAYMDCVRAVDFLCSRPEVDTSRIIVDGQSQGGALSFA
                     TAALDDRIRLCVPGVPFLSDFPDYFKVAIWPGNEFKDYVNNHPETTWEEVFRTLSYFD
                     IKNLAPKVKAPVFMLVGLMDETCPPHINFAAYNNLQCPKEYIIYPYSGHGLPAENYQA
                     RMNWVRQQLNLK"
     gene            complement(15770..16540)
                     /locus_tag="PLO58_02960"
     CDS             complement(15770..16540)
                     /locus_tag="PLO58_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQO73781.1"
                     /translation="MLLTLISWNVNGIRAIHKKGFLDWVKATQPDILGIQETKAEESQ
                     LPPDLAQISGYRSYFSSAIRKGYSGVALYSRLEPVSITQKFGIEKFDTEGRLLIADYD
                     QFVLFNVYFPNGQMSEERLRFKLEFYDAFLDYVDRVNVSGRNIIIGGDVNTAHTEIDL
                     AHPVANSNISGFLPEERAWLDKFLSHGYYDTFRLFNNEPGNYTWWSPFTNARERNVGW
                     RLDYFFVNKRFVANVRAAEILADVAGSDHCPVSLQIEI"
     gene            complement(16544..17839)
                     /locus_tag="PLO58_02965"
     CDS             complement(16544..17839)
                     /locus_tag="PLO58_02965"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HQO73782.1"
                     /translation="MKKIKYIVVGALLVIVSQGYSQLYMNPRSISFADAYATQARGGD
                     VIGWNPANLGLNDNPKFQFNFGIIPIVPFPALQISNNTISTHLLNNYIFTGDYLDDDA
                     KEELLSHFPDGGLNFNPLVQMRFLNFSVNSWAFTLGAEVTGKVVAPKSLFRLALFGNE
                     FEEPIDLSDTDLDLQSVVTLGIAHGWQIDNVPYLSNYVDKFSVGIAVKLLAGAGYSGF
                     DDLTANVTTYKDRIILEGDLKGEYGLGGAGLAIDIGAASVINEKMTANLALNNVVGFL
                     NWGIGEARKAEYSFYADIPSSDFGDIDSIMDESLRKDTTYTVSGIRTNYPAYMIMGFE
                     YRVLQNLRLFVNYRQSFSNDMASTTTPAFSFAAEFLPADWLPIRFGVGFGGNDKLKWG
                     VGSGMTFKHYRLEWGFAQTGGFFNHGKGLAFCLDQSLVF"
     gene            complement(17844..20801)
                     /locus_tag="PLO58_02970"
     CDS             complement(17844..20801)
                     /locus_tag="PLO58_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73783.1"
                     /translation="MQKFKIIIIAGVAVLFSFCDLKIPSESDYPTWSVNLNVPLLNET
                     VTVNDLLEDSLIVKLPYGTAGDSIFAYEDQIPIEEVRVGNKLNIDDITQSIQQGVDEV
                     NIAASDKKYSSGFDEVGVEPVSEQNSNLIGDIHLADTDVESTAPILFSEIVDMTGIAD
                     GASKTIAISTPFPTINRQIVFNNFNNANFIGGFLEINIRNNLVVELGAPVYVRLLDGS
                     GNPIVGTDGDSAKAIWTEGLASGQSSKKTINLTNKTLPGTIIVEVSGVICGSGSQIVT
                     KNSATLNSSFVVEVQAKDISVSSAEAIVPEQTIDTTGTIEFAEDEPNKVETAVIKDGH
                     LKISIQNDLPVSANLELTIPSLKTASGGSDFYQVIPMAAGQTVIQEYLLTDHKLEMDI
                     DEQEVNYSYIVRTISTDPEMVDISSSDQVNVNIDMYGENSESDVTFSEIKGIVEPQNI
                     KDSGEINTASDARISTAQIASGGMTFSIENGINIGTTGIPHLVLTFPEILTTAGTPVT
                     IESDIPGGQTTINYDLSNCLIQPLSESVTADSVRQYITYKSLVTTSSGEIAEYSLIDS
                     IKFDINTSEMTFSIVTGYFDQEAIVANEVLELEDKTKLETAQISNGNLILTFVNNIGV
                     FADVHFTINEIVHRTDGTPLERIIHLPNSSKPVIEAIPLDEYNIQLPFTDLTTNQEIH
                     YTSRVSIPSDREMTITFGQEINVDVLLTEVEFASVSGYIDTVQVDIEPSENEVNALPE
                     EFDGINLKNVEMAIEFNTDIGVPVELNLFISSFNDEGESVRREIHQVITENPRVVIPD
                     AEELINIKPKKIVTSGYALVYGTGQVDTAQYVSGNISISVPFEMEITEDAKMEFDPEL
                     VEEDIPEEIESAILYAEVENGFEIGGKLILLAAQDTLLFDVAPPYGPDTLAVINVHPD
                     SSFREVVELGEKQIALFQDSVYVKTQFNLVGRTDEMGNPIPSRFMKGDELKILLYGTI
                     NGLIDFADRKEED"
     gene            complement(20938..22836)
                     /gene="dxs"
                     /locus_tag="PLO58_02975"
     CDS             complement(20938..22836)
                     /gene="dxs"
                     /locus_tag="PLO58_02975"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQO73784.1"
                     /translation="MAEQEAKKYPRLEEVSSPADLRKLSVDELVSLSTELREYIIETV
                     TNTGGHLASSLGTVDLTIALHYVFNTPTDKLIWDVGHQAYGHKILTGRREGIKTIRQY
                     GGISGFCKMSESEYDCFGAGHASTSISAAVGMAVARDLNHENYRIAAIIGDGAFTGGL
                     AYEGLNNAYNINGQFLVILNDNEMSISRNVGAMSNYFTRIVTSPSYLNFKNQVWNSLA
                     LLPRGTNFFRKAGRKLLESFKNFLAPGIIFEEFGFRYYGPVDGHNIQQLVNTLNNIKD
                     LKYPVILHIITQKGKGLASAEIDPIKYHGIGPRKDVNACVSTPTAPPFLNAFSKIACE
                     IGEKYPKSIFITAAMAEGTGLVEFARRFPMRYFDVGIAEGHAVTFAAGLAACGFRPVV
                     AIYSTFLQRAYDNIIHDVALQNLPVVFVLDRGGIVGEDGPTHHGAFDLSYLCTVPNMI
                     VAAPRNGTELRNLMFTALEQSDAPFAIRYPKDSYVEFDDQAPAVPYQIGKWEIIQNGQ
                     DVAILSVGVMTNNAIEAACLLKPKNIFPTIVHARFVKPLDTQMLSEIASKHRIIITIE
                     ENSIVGGFGEQVARFFAEQNLPQKINILAIPDRFIEQGAREILLRNLALDAVGIANKI
                     EQIYHKLE"
     gene            complement(22836..23405)
                     /gene="pdxT"
                     /locus_tag="PLO58_02980"
     CDS             complement(22836..23405)
                     /gene="pdxT"
                     /locus_tag="PLO58_02980"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HQO73785.1"
                     /translation="MNNKAKRVGVLGLQGAFQKHLKIINSLGAEAIVIRYKEQIEKCD
                     GLIIPGGESTTMSRLIDEMDLRESLEKFPGMIFGTCAGAILLSTNGTDPLVKSLGRSS
                     VKVKRNAYGRQIESFVENVDLTFSDKPFKGIFIRAPKLDHLDSSVEILGHLNGDVVLA
                     RSGNNLMATFHPELTDDFRIHQYFLNHLQ"
     gene            complement(23439..24320)
                     /gene="pdxS"
                     /locus_tag="PLO58_02985"
     CDS             complement(23439..24320)
                     /gene="pdxS"
                     /locus_tag="PLO58_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HQO73786.1"
                     /translation="MDKGSFEVKVGLAEMLKGGVIMDVTTPEQAKIAEEAGAVAVMAL
                     ERIPADIRAAGGIARMSNPKVIKAIQDTVSIPVMAKCRIGHFAEAQVLEALGIDFIDE
                     SEVLTPADEENHIWKHDFKVPFVCGCRDLGEALRRIGEGAALIRTKGEAGSGNIVEAV
                     RHMRRVQSEIRKLTTLSDDELMAEAKRLGAPFELVRQIAKTGKLPVPNFAAGGIATPA
                     DASLMMQLGAESVFVGSGIFKSEDPAARARAIVLATTYYNNPEKIAEVSAGLGEAMRG
                     LDLAEIPENERLAKRGW"
     gene            complement(24364..25203)
                     /gene="ispH"
                     /locus_tag="PLO58_02990"
     CDS             complement(24364..25203)
                     /gene="ispH"
                     /locus_tag="PLO58_02990"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQO73787.1"
                     /translation="MALKVNISATVGFCAGVQNAVKKAQTAAQKFGRVAMLGDIVHNE
                     KVIRELTDLGVEVVQNLDQIEPTVPVLFRSHGTPRSIWDEAHQRGLRIIDATCPLVRK
                     IHNEVQMLAKEGRQVIIIGDKNHEEVSGIASQINDPIIVSSPEEAERLPKMKKIGVVA
                     QSTQMIENVSAIVAILAAKTDDLRFMNTICRPTRQRQEQIRELALTNDVMLVVGSKNS
                     ANTRRLAEIAKKLNTNTYHIENAEQINPDWFINKTSVGVATGASTPVEVVEEVVKKIT
                     NFD"
     gene            complement(25203..25763)
                     /gene="dcd"
                     /locus_tag="PLO58_02995"
     CDS             complement(25203..25763)
                     /gene="dcd"
                     /locus_tag="PLO58_02995"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HQO73788.1"
                     /translation="MSVKSDRWIEEMALKHKMIEPFEKSQISSGVISFGVSSYGYDMR
                     VADEFRIFHGRKDTVLDPKQITTDLFIDYKGAVCTIPPNSFVLGRSLEYFRIPRNVIA
                     VTSGKSTYARCGIFVNVTPLEPEWEGFVTISIANLAPVPVKIYANEGVAQVIFLESDE
                     ACRISYADKKGKYQAQQNITNAKIKE"
     gene            complement(25765..26766)
                     /locus_tag="PLO58_03000"
     CDS             complement(25765..26766)
                     /locus_tag="PLO58_03000"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HQO73789.1"
                     /translation="MEKYNWQKIVEKIKESDKILLTTHHNPDGDGLGAEAALYHCLKH
                     LGKSPFILNSSPLPPEYSFLNEGEIFHTYPNELDQVELTDFDLLMILDASHTERLGAL
                     GPVLKSLNIPTICIDHHPFNNCLPENISVIDDKAPATVALVFELLKIIAPQAIDQKVA
                     EALYVGLMTDTGSFRFENTTAETMLLAAELLRYNVQPGLIYHYVYENYRPERMKLLGM
                     VLQNVQYEFENRLAWFCVTRAQVAAAGAYLDEVDGFTDVVRSIRGVEIAAMFLEERDN
                     RTRMNFRSKGHYPINNVARQFGGGGHPYAAGLVMDVGLEDAVKTVLPALRKVFLDEG"
     assembly_gap    27220..27319
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27320..27687
                     /locus_tag="PLO58_03005"
     CDS             <27320..27687
                     /locus_tag="PLO58_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HQO73790.1"
                     /translation="QIYKCEICGNIVEVLHEGAGELVCCGKPMKLIVENTTDAAREKH
                     VPVIEKIADGYKVSVGSVLHPMIDVHYIEWIELDADGQVFRKYLKPGDTPVAIFNVSA
                     ENVTAREYCNIHGLWKGTL"
     gene            27687..28175
                     /locus_tag="PLO58_03010"
     CDS             27687..28175
                     /locus_tag="PLO58_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HQO73791.1"
                     /translation="MLSPKLLTAFNDQIKHELESAYLYAAMNAWFKSENFDGMAHWME
                     VQTKEEINHARKFFDHIFERGDVVELQPINILAKKWASPLDAFKAAYEHEQFVTGKIN
                     DLMTMAIADKDYAAQEFLNWFVAEQVEEEDSTLKIVQLLKRIGDHGQALIMVDRELGK
                     RE"
     gene            28192..28674
                     /locus_tag="PLO58_03015"
     CDS             28192..28674
                     /locus_tag="PLO58_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQO73792.1"
                     /translation="MNFNIDEVFEIAEQIEVNGSRFYRQAAELSQLAEHKNMLLKLAE
                     MEDQHQKTFAGMREKIKQTKQNLSYDPNQEAALFLQAFASGYVFDPTADSSALIRDQK
                     SIADILHLAIGLEKDSIVFYLGIEKILLNEDDIVHVRAIIKEEMGHITLLARKLDELN
                     "
     gene            28821..>29254
                     /locus_tag="PLO58_03020"
     CDS             28821..>29254
                     /locus_tag="PLO58_03020"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HQO73793.1"
                     /translation="MAIRIFVTGGTFDKEYNELTGQLYFKDTHLPEMLILGRSRVEVE
                     IRTLMMIDSLEMTDDDRLLIAHHCQKAAEDRIVITHGTDTMVETARLIAQNVSGKTIV
                     LTGAMIPYKFGSSDGLFNLGSALAFVQTLSPGVYIAMNGRFF"
BASE COUNT         8525 a         6771 c         5701 g         8157 t
ORIGIN      
        1 acatcgttac tccgccgcgc cggtgacctt ttaacatagg tatattaatc aaatactccg
       61 catcctcgag aattgcgtaa acattatcgt cataaacggg accttctccc ggccggttgg
      121 catcccacac attttctcgc aaaatcgttc ctttgtcaga ataataaatc ttggtctttg
      181 taccaacctg tgcatgttcc cgtcctagag aatcgggatt atcatgatcg agatagtgta
      241 cagcgggaaa ttcgctatgc cagtaatcgt atagatgttt atagatgtgt ttcatcggat
      301 caccgatata aatatcgctt tcggcaacgc cgacaacatt gaccagatgg cgtaaaaccg
      361 tcatcactac cgccggtgaa gtctcggaaa ttccgtaact agagtttttt gcacatgata
      421 aatcggtcgt gctgtaatta ccgccccagc cgctggtggc atttatcttg atgaaaattt
      481 tctcaccagg tgaataatca actgcgcctt tttcccgagt tgtattgtga tatttgaata
      541 tcgcaaccca ggcggcacta tcgctggtcg cgccagttaa agactggatt gctgaagaaa
      601 ccatactgtc aactaccgcc tgattattat tctggggcaa aaaccaggct tttccagcac
      661 gataagttgt ataaccactc ccccaccaag aactaacggt tgaatccggt acgcaatttt
      721 cattggtagc atcaggatta taaacccaga caacccgacc ggggaaaatg cctttagccg
      781 ttcccatcgg aatattggct tcgtgttcaa tagttgcata agtggcgtaa gcagtctctc
      841 gggaattatg aaaaatgagc aaggaagcca ataatcccag aataacgaat ccagtcgcta
      901 ggaggtagcg cgatttggct aacatttttt tggctttcat aaaagctgta gccgaagcaa
      961 ttaccccgat taaataaacg ataaatcccg aagcgagcgg catagcgacg cgcatacaag
     1021 gataagttgc acgagtcggt ttgggaagca cacgaactac cacccagatt aatgctaaca
     1081 aaccagttat tggaaataat aacttcgacc aaccactaag cggtttaatt cctctgacct
     1141 tgccggtttt tgggcaaatc aaaaagaatc gttgcaggat cttcttcatt agtttctctc
     1201 tttcagattg ttagtgatta tcaaataatt ttattctgca tttattagca aaataaaaat
     1261 tttattgcat ttctacaaca actttatttt cgttctgaat tatatccagc ggtattagat
     1321 ttccggttaa atcttgattg tttataatta gttttcgaat gcctttttga gtaccgttcg
     1381 ggttttttat taaaatatta aacattttat tccgaaaacg cctcgtaatc tgaatctctt
     1441 tccagtttga aggaagacat ggatcaatcc gcaaaccgaa ataatccggc tgaatgccga
     1501 gaatgtactg agtggcagta taatacgacc aggcggcggc tccggtgagc cacggcaaac
     1561 gtgaagcccc cgcgcgtgga ctatagcgac tattcgtaaa ctgacaataa acataaggtt
     1621 caatctgccg gatttcagct ctggtattat aagccgccgg tagataagcc cgtaaatact
     1681 ggtaagctcg atcgccgtgt cccaacatag tttcagcgat tatcgcccaa ccctgagtat
     1741 gcgagaaaat cgagccgttt tctttcatac cggcattgaa aagcggcgct ttgacgatgc
     1801 gatggtcggt tttgatatac ggcggggcta aaatcatcaa accatgttcg gttgccaagc
     1861 gctcataaac agcttgcatc gccgtctccg cctcatcttt atcggcaaac ccgctgatta
     1921 ccgcccagga ctgtggattg agccagatcg aaccctcttc atcagtatgc gaaccatatt
     1981 ttagaccatc atagcgataa gcccggagaa accactgtcc gtcccaggca tatttacgca
     2041 aattttcatc caatttttgc aattgctcca ctgcccactt tttctcactg agtcgctcga
     2101 gcaattcaca aatttcaata taagtcttta gggcataacg caattgaaaa gcgacgaaaa
     2161 tcgtctcgcc acgttgaccg agttccagac aatcattcca gtcggcataa agtccacagg
     2221 gcaaaccgtg agtaccggtt cgttccaaat taaattcaat cgctcgtcgt aaatgaccga
     2281 gcacggtggc ttcgccttca tcagcaaacg gtaaaacttt gtcgaaaaaa gacaaatcgc
     2341 cggtttcttt aacataagcc ggaacggtat tgaataacca catacagtca tccgaacggt
     2401 attcttcctc cttgggcgga atttcccggc caggatgatg atcaaacggc ttcactactg
     2461 acatagcgcc accggtggaa acctggccag tcagcattaa ttcgagacgt tcaccggctt
     2521 cttcaggaat caaaggcaaa acccccagta aatcctgcac cgtatcacga tagccgagcc
     2581 cgtcgcgttc accactataa atcaagctcg cggctcgcga ccaggcgtaa gtaatcaaac
     2641 aattaaacgg actccagaca ttggtcatac tatcaaattc ggaatcgggc gtttggacta
     2701 aaaatccctg caatcgttgg tgccagtgtt gctttagttt ttcaaattca atgtgaatat
     2761 tatcgaggtt ctgaaactcg ctcaaaacct tccgcccctc ggcctctgcc cttccaatac
     2821 ccatcagaac cgcaagttcg cgcgattcac ccggcttgag ttcaagatca atctgcaaaa
     2881 cgcctacgcc gttatcacca acggcaatgg aattaagaca ttcgcccttt tccaccacaa
     2941 tgggattatg gtaactgcga tagccaccga ggaatatttc ccggtcggta tcgaaaccgc
     3001 tgacttctgc gcccacgact cctataaaag tatgtttatt gccatcgtga agccagagat
     3061 cgccagtatc gtccgccaga ttgacgttga tcccgtggtc gatgatattt ccgactacat
     3121 tcatcctaag aatgaactga gtatattgca aattaaaagt atcctgccat acgtgccaat
     3181 tattggtgta ttcaatgaaa gtaaagagac gcagacgtct tgtaacatta tcacggttgt
     3241 gaattttcaa taaccaacat tcaaaatgcc ggttcaaagg cacaaaataa gtcgtctccg
     3301 tcaaaatatt atcatattcc gactcgataa tggtataagc cgtcccgtgc cgacagacgg
     3361 ttttaaattt gtcaagtggt ttgccaaccg gttgccagga agtcgaccaa taatcatgct
     3421 tgtcaaggtc gtggatataa aaataacgcc ccggttgatc catcggaatc gcattgaaac
     3481 gcagacgcat gaatcgccct tgcgccgccg actgaaaaaa actgtagcca ccggcattgt
     3541 tagtaattat cgcaccgtac tcggtcgaac caagataatt gctccaactt cgcggagtat
     3601 ccggccgatc aattacatat tctttattct tatcgtcaaa atgaccaaac tgcatattta
     3661 gttactccac tattttaaat taaaccgtac cgcgtcgcgc ctcaagagct gaccggatct
     3721 ctgcgcattt ctgccgagtg aggtttaccc tggttatgaa aaaaatcact aaaattaatc
     3781 ccacaacggg aatgcaagcc agcatcgccc gaatccagaa aatggtctcc ggcgcttgaa
     3841 ctgcaagact agaatcgaaa tgggcaaaat taaggacact tccggctaaa aatccaccca
     3901 atgaattgcc caacttaaga atatatgagg aacaaccggt gaatgaccct tctcgtcttt
     3961 taccggtatt aagctcatca taatctataa tatcggcgcc gatcgagccg tgaagcatcc
     4021 agagaccagc tgaggaaagc cccattagac cagacgcaat tgtctgaagc cagggaacaa
     4081 gtggcgtata aagaaaccag gaaccgccat agccgataat tcctataatt gccgccacca
     4141 ttacggcttt gcgcttttct agatagtagg cgactcggtt taggatcgga cctcccagca
     4201 atcccccaat cataaaagca atgcccatcc agaaattcca attatcaccg acgatgttat
     4261 taccaccaca tacataatgc acggtagtgt aatatcccag cgttccgacc atactcgtgc
     4321 ccagagtaaa agaaccgccc agcaagagca tcatccgaaa cggctggcat ttgagagttt
     4381 cataaaagga acttgccagt ttgatacgtt ctttagtctt gacaactact ttctcgtagt
     4441 aacgttcctt tactcgaaaa aaaataataa tggcgaaaag tgccatcaag acgccgatga
     4501 tagaggtata aacttgcatt ccccgcagag tattttttct accggtaccg ggaataagaa
     4561 accatgcaag attagtaaaa cgcagagcat agaaattccc gacctcgaaa attttttgca
     4621 tcacactacg gtatgtcatc actgaagtac gttcttcata atcaggagtc aattcgtttc
     4681 cgaggctgtt ataaggaacg gtaaataggc tgatgatcgg aatatagatg aggttcgaca
     4741 gtaacatgta ccaaaaaacg accgataaac cgagaaacgt ttgatcagcc caggcaggcg
     4801 acactaaaaa tagcagtggc aatccgaggc cgctaataac gcctgctcct agaatgaacg
     4861 gtcgcctgcg gccgtgcttt gaacgaaaat tgtccgaaat ccagcccgca attggatcag
     4921 taaaggcatc gtatattcga attagcgtca gagccaatcc aaccaacatt ggattaacgc
     4981 ctaaatacat attgaaaacc gcgaacgcga cgccgggata gagccaaaat ccccacatat
     5041 ctaatccagt tcctaaacca taacaaattt tggttaaaac cggaaggcgg tcgcgtacct
     5101 gcgtagttga attatttgcc gttatttctt gtgccaattt cgttccttta gtaaaacaat
     5161 ttttctggca aattcagggt tctatcatac ttctgaatta ttcttgatat aatcaatcaa
     5221 ttcatcagtc tgagcaaatg ccagagccgt ataggtatcc gccgcaccgt aataaatcgc
     5281 aatccgaccg gtggcagtgt cacacaagtt ggcacacggg aagacaacgt tcggcacgaa
     5341 accggtggtt tcgtatggtt tttccggcgt caataaatag tctcgtgtcc gatataaaac
     5401 tttactaggt tcatcaatat ccagaatcac agccccaaaa ctataaacaa aaccgttaca
     5461 agtcgttgat actccatgat aaaaaagaag ccagccttcc gaagtttcga ttggaattgg
     5521 accggcacca attttcgttc cctgccacca acccatcccg cctttgccca tcacgaattt
     5581 gtgattaccc cagtaaatca ggtcgggact ttcgctcagg aaaatatcac cgaatggtgt
     5641 atgtccactg tcactagggc ggctgagaag tagatatttc ccattcactt tgcgaggaaa
     5701 aagcaccccg ttacgattaa aaggcatcag cggattcggc atcctggtga atgttttaaa
     5761 atcctgcgtt ttacctaaac cgatcgaagc cccgtgcaga tcatcacacc agacgatgta
     5821 atatatatcg tcaattttca ctaagcgggg atcataacca taactgagag gcttgggatt
     5881 accttgctca tccacccagt taatcggatc cggcccgatt tcccatctta atccgtcgtg
     5941 actcttaccg gaaaaaagtg tcgctcgccc atttttctga tcggctcgaa aaacacccac
     6001 aaactcactt ttatatgaaa taactgcact attaaaaatg cgggcggctt tcggaatggg
     6061 attccacccg attagcggat tcttactaaa tcgccagact atatccgaac aaccttccgg
     6121 cctctcttcc cagggcaaat ttggaatcga gttaccgact attcgatatt tcattgtgaa
     6181 attccttttc ttttataatg ctttaagctg gagctattgt ttctccactt tgcgcccgac
     6241 gctctttcaa atctgctccg ataacagcca tacgctgatt gttcaaagga taaaatgaaa
     6301 caattatcaa agccaaaata ccaaatgccg ccggaatcag actaaagagc aaaagtaacc
     6361 ctcgcaaact ttgcggcgtc tgagcctgat ttggcataaa tcccaaccgc gacattaaat
     6421 taagagcaac aaaagcccca aacgcccagc cgaatttctg cgacatcgtc gaggcagaaa
     6481 acaccaatcc ggtcgcccgc ctaccatttt tccactcagc ataatcagct gagtcggcat
     6541 acatcgccca gatcaaaaca ctcagaggcc caccggttaa tgaaccgata atctggaata
     6601 agaaaatcaa accgatttgc tccggcgata aaaaataata gatactggta ctgaaaatcg
     6661 caataacgaa aatgatcata aaagccggct ttttccccac ttttttggca aaccagctaa
     6721 ctaacaaaac cccaataaat gacgaaatct gaccgacggt attaaaaacc gaagtaagtg
     6781 tttcgaaatc ccaggtttta tttttaaaaa acggcaaaga aacctcacgt gttccgacaa
     6841 tatatttaaa ataatgaact gtcacactgc ttctaatggc aacgaataga ataaatgtta
     6901 tagtcgtaaa aagcaatatc aaccagggcg aatttgttac taattcatat aaatcctttt
     6961 ttattgagga tttttgtgac tttggcggat gaacacgctc gcgcgtgcct ttgaaagcaa
     7021 tcaggaaaaa gataatagcc gcaattccat aaatcacaaa tgataactgc caaccgcgcg
     7081 cttcgttgcc tttaccaagt gctttcacca tcggcagaag cgtcgctgac acaataaccc
     7141 cagccgtaaa cgcaaacaaa aatttgatcg atgatacgga tgtgcgttcg tccgaatctg
     7201 gcgaaattac tcccagcaat gctgtatatg gaatattgat cgccgtataa agcatcataa
     7261 tcaggataaa agtcacataa gcataaacga ttttacccgt catcccaaag ttaggcacgc
     7321 tgaatgtcag aacaccagcc accgcaagcg gtacacaaaa ccacaataaa tacggtctga
     7381 atttccccca gcgggtgttg gtacgatcag ctataattcc catcaacgga tcgttaacgg
     7441 catcccagac ccgactgaca agaaacatcg tagccgctac tccggctgga atcaagaaaa
     7501 catccgtgta aaaataagtg aaatatagca taaaagtctg ccagtaaagc accgacgcca
     7561 aatcaccaaa accatatgcg attttttccc gtaaggtaag atacttagcc attgtcctct
     7621 ccttttttta aaattttaat ccgtaatatc ttaatttcac tccattataa agagtctatt
     7681 atccccctaa acagacagtt ttatctttaa atttttaacc catatctacg attacttcat
     7741 tggtattttt cagcttagaa caaggaatta gattattact caatgcttca cggtttaaag
     7801 ttatccgccg cacacctttc tgcactccgg caggatttct tacttcaata tttaaatttt
     7861 tcccccggaa tagacggcgc actgtgtagc ttttccagtc ggatggtatg cagggatcaa
     7921 tcagcaaacc gtcaaactgt ggtcgaattc ccagaatata ttgcgagacc gccagaaaat
     7981 tccaagccgc cgtgccggtc agccaggaat tcttagcttc ccccggttta taggcatcct
     8041 taccagcaat catctgagca tagacatacg gctcagtgcg atgtaaatcg ctgatatcct
     8101 cgagaaacga cggacagatt ttacgccaat attcaaatgc ctgctcaccg ttacctaatt
     8161 tagtttcagc gatcataatc cagggattgt tatgacaaaa tattccggcg ttttccttat
     8221 aaccaggaat gtaagtcgaa atctcaccta attcgatgta atatttcgtg taagccggat
     8281 tttgcaaaac aattccaaat tcacaatcga ggtatttttt tacggaatcg agtgcttttt
     8341 gcatccggcc gtcttccttg ccgatctctg ccatacaaca gaatccctgc gattcgatga
     8401 aaattttgcc ttcctcattt tcctgactgc cgactttcct tccaaaagca tcataagcgc
     8461 gcaaaaacca ttcaccatcc cagccgaacc ggtcaatgct ttttttcatt tcatcaatat
     8521 gctggcgggc ttcagtggct tcatccattt ttcccagaaa ttcacaaagt tcaacgaact
     8581 cctgaccata tagaacaaat gccccggcaa tcataataga ttcagcagtt ttaccgtcgg
     8641 tttttaatgg accggtttga aaagattgat ccggatcagt gttaaaaata ttaagattaa
     8701 ggcaatcgtt ccagtcggcg cgcccgatta gcggaagttt atgaggtcca agattattta
     8761 ccacatgata aaaagaacgc ttgagatgtt cgaacaggct ggcttgatta ttcggatcat
     8821 tatcgaatgg taccgattcg tccaatatcg caaaatcgcc ggtttctctc aaataagcca
     8881 caactccaca gattagccag agcgggtcgt cattaaaatt gtcgcccact tcataattgc
     8941 ctcgcttgct taatggctga tattgatgat aagcaccgcc gttcggtaac tgagttgccg
     9001 caatatcgag caaacgttcc cgcgctcgct ccggaatctg atgaacgaag ccgagcaagt
     9061 cctgactgga atcacgaaat cccattcctc gtccaattcc ggactcgaaa taagatgcgc
     9121 tacgcgacat attgaacgta accatacatt gatattgatt ccagatattg acaatccggt
     9181 caatcttttc ctcgccggtt tggacttgaa aaaccgataa taaaccatcc cagaattcgc
     9241 gtaaatcgtt catagccttt gtcacacttt ccggcgattc gaagcgagat tgcatctgac
     9301 gcactttggt caaattgatt ttgccgtcgg cagtaaactt ttcagtttcg gggtattcta
     9361 catatccgag gataaaattg aaacttttcg attcgccagg attgagctta acctcaattt
     9421 gatggacggc taccggcgac cagccaaaag ccaccgaatt gcgatttttg ccctcgaaca
     9481 cggctaccgg ctcgtgtggc ccattatgca atcccataaa ggattctttg tcacagtcaa
     9541 aaccatcaat cggggcgttg accgaataaa atgcaaaatg attgcgacga tctttataat
     9601 cggtaaaatg caaaattgtg ctaccgatcg cttccgctct accgatcgag aaggtgcgct
     9661 gaaaattagt catatcttcc caggcattcc acaagcaaaa ttctacatag gaaaaaagtt
     9721 tgatttcctt gacggaagta ccggtatttt tcaaggtaac ttgctgaacc tcaccgtcga
     9781 aattcatagg cacaaaagcc agaacttcag ctaaaatatc attgcgctga ccggaaatgc
     9841 gggtataacc taatccatgg cgacattcat aaaaatcgag cgctttctta accggtttcc
     9901 agccgggatt ccaaacatcg ccattatcgt tgatgtaaaa gaatttaccg ttgtcatcca
     9961 aagtggaatt attataacgg tagcgcgtta tgcgccggag gcgagcgtcg cgatgaaaac
    10021 tatagccacc gccggtatgc gaaatgatgg aaaaaaaatc ctgactgccg agataattga
    10081 tccagggata aggggtttcc ggcgtcgtga taatatattc cctgtcttga tcgttaaaat
    10141 gaccgaattt caaaactaat ctcctaaaat atccttacac gtaatatccc aataatcgaa
    10201 acgtttatac ttttagaatt tataatagtt cgatctcttt ggaaagtcta atatcttgtg
    10261 acgaagaacc tatcaatata acaaattttc ccttttcagt cgtccaattt ttaattgcgg
    10321 gactccagaa ggcaaaatcc ttttggctta atttcaaggt tacagtttta atttcacccg
    10381 gctctaacac tattttctga aaacctttca attccttcgc tgggcgaggt aaggatgatt
    10441 cgagatcact aacatatagt tggacaattt cggcgccctg gcacttaccc acatttttaa
    10501 ccgaaaaggt gactgaaacg gtatcaacct cggaaaattt ccccggtgtc acttgcaaat
    10561 cactataaga aaacgatgta aacgacaggc cgtgtccaaa gggataaagc ggttcgattt
    10621 tctgagattc ataccaccgg tagccgataa atattccctc tttatatcgc acatcatata
    10681 ccggatgcgc tctctcgcct tccccattcc aatcgaaata aagcgattcg ccttccggaa
    10741 tataaccgta acccggtgaa tctttgaact ccttttcaat ggtaattggc aattttcccg
    10801 aaggattaac cttgccgatt aaaatttcgg ccaaggcttg attcccaatt tgcccgggat
    10861 accaggcata gattattgct ctggctttct cattccagcc agtcatacgt atcccacttc
    10921 ccgaagtcac aataacgacc gtgttcggat tattattgac acatttaaga actttctgct
    10981 cttgatcttc cggcaattca aatgggcgat cccaaccttc gctatcacta gttcctacat
    11041 tgcacagcac caattgggcg gatttgatct gctttacggt tggatttttg acatatttta
    11101 tccgatcacc aaaaactttc tgcagttcag aaatcatttg acggttattg tatccctcaa
    11161 catacgccga accaccacca acggctaatt ttttgacata atcgccggta actaagatat
    11221 tttttacctg ttcttttacc ggcaaaatgt tgttttcatt ttttagtaac actattcctt
    11281 ctcgggcggt ttgcaaggca actttttcat gggcttcata gaaactgtct tccaccgcag
    11341 ttcgcgaccg accatataat ttcatagcaa aacaggtgcg caagatactt tgcgccattc
    11401 gatctatgtc gctaacgtgc actttattat cctcaagaag ctgatgtaca ttttccagcg
    11461 caattgtgaa cggcatctcc agattctgac cggatttagc cagtttctca ccgttataaa
    11521 ctgaccacca atcggtcatt accagccatt gaaatccaag ctgatttctc aagagataat
    11581 tgatgacata agcgctttct ccacaccatt caccatttaa caaattataa gaagtcataa
    11641 ccgcctttgc gccagcttcg attccggctt tgaaggcggg catataaatt tcatgcaaag
    11701 ttcgttcatc aacaaccgaa ttgcttttct tccgatagta ttcggtatta ttggctaaga
    11761 aatgttttag ggtggctacg gttccagtac tctgaactcc ctgaacataa ttctctatca
    11821 tccgcgccgc taagtaggga tcttcgccaa aatactcgaa attgcgaccg cattgggatt
    11881 ggcgataaat attcatccct ggtccaagta aaatgccaat attggcggct ttacattctt
    11941 caccgatcgc tcgagcgtat tgataagcca attcacgatt ccaggtggcg gataatgaaa
    12001 tcggacaagg aaaggctgtt gatttttccg gctggtaatc cgagaggtca atttcattaa
    12061 agctctgacg aatgtggatg ccttgagtcg catccgacat ataaatttcc gaaagattca
    12121 aacgcggtag cgctcgaata aagaagtatt tatcgccgcc aatcaaagca attttctcat
    12181 ctaaggtcat ttgagccaag atcgattcgg ctaatttatc tgcttctgca taggctaaag
    12241 gctgaaacgt ttccatatct cgattaaatt cctgactgaa taaacgatta ttggtggcga
    12301 acaggtttag taaaattaga aaaaataaaa aaccacctaa gtaggttttg cgcattataa
    12361 ctcctgtaat taatttggtt caatttattc tcttcgtctc atttaatatg tgataatttt
    12421 cgggaaattt gagttgttcc aatttcggaa tcggctaaaa atcgtaatat atatacccca
    12481 ccggcaattt cgtcaatcgc accgttcgtt tcccaggtgg tttggtaacg tccggcggga
    12541 aacactttat ttagcaaagt gcagatcaaa ttaccgcgta aatcgtaaac accaagatta
    12601 accccggcgc taactggtaa ttcgaagctt atctgggtag aagaattaaa gggatttgga
    12661 taattttgag ctaaggcaaa tccggctgga ataagtcgtg tttttctaac cagattgggt
    12721 tgtttggtat tacctctgat gatataatcc ctgattaata atcctgattc ggtaatttcg
    12781 gatgccgacc aaccgccgtt agccgaggcg cctggtttta aggcggctga ggtttcattt
    12841 ttatcggcaa ttgaccaatt gcaccagctg atttgattac tttccataaa attcatccag
    12901 aggcgggttt cggcggtatc aatcggacca tcgccggtat tcataacagt accaaattcc
    12961 gtcacaaaaa gagcgatccc attatttagt gctgttgatg ccttattgcg taaggtttgt
    13021 ttatgatact gataagccgc ataaaaatgc aaagcatagg caatattatt ctgcgagagc
    13081 ggattttgcg aggcaatatc tacatcctgc gaccagtttg gtgttccgac cacgattaaa
    13141 ttgtcgggat caatcgccct tattgaatca actacggcgt tcaaataagg ctttagtacg
    13201 gtcttccaat tgtcctgttc aggttcgtta aatacttcat aaatcacgtt gggataggcg
    13261 ccgtaatcag tagcaatttc ctgaaagaaa gcaatcgcct gcgcctgatg ttcatgagca
    13321 ttatgatcat gccaatccac aatgacataa attcctaaat caatacaagc ctcgatgacg
    13381 gtcacgattt ttgccttttc gcttgcggga ttgttcaaat aaccacccga ttcgataccc
    13441 attgccgccc ggacaacggt gcatttccag tcatctcgaa gccatttaat acaattgtaa
    13501 ttgtagtatt ttcccatcca ttggctccag aagagactca ttccgcgcaa cactatcgga
    13561 tcaccatttt gatcaataat gcggttaccg ctgacagtaa ggtttccatg ctcttctaca
    13621 aaagttaatt cggaaccacc gatttgatcg gatttattat tctgctcaca ttggatggga
    13681 atttgtaata taactattaa ccaaaacact ttaataattt tacacttatg acgattatgt
    13741 ctctgaactg aattcatttt taaaaattcc ttttagcgat tgagtttttc acccatcaat
    13801 catccctatt tatttacaac cggaaaatta cctttatatt gacaacgata gtgatatttg
    13861 ttgattactt cagattcagt tgctggcgaa cccaattcat ccgcgcctgg taattttctg
    13921 ccggcaaacc gtgtccggaa tagggataaa tgatgtattc ttttgggcat tgtaaattat
    13981 tataagccgc gaagttaata tgcggcgggc aggtttcatc catcagaccc actagcataa
    14041 aaaccggtgc cttgactttc ggcgctaagt ttttaatatc aaagtaactc aatgttcgga
    14101 aaacttcttc ccaagttgtt tcgggatgat tgttaacata gtctttgaac tcgttaccgg
    14161 gccaaatggc aactttaaaa tagtcgggaa agtctgataa aaacggcaca cccggtacgc
    14221 aaaggcgaat acgatcatct aaggcggcgg tcgcaaacga aagagcgccg ccctggctct
    14281 gtccgtcaac gatgattcgg cttgtatcaa cctctggtcg cgaacacaga aaatctaccg
    14341 cccgcacaca atccatataa gcgccgcggt aaatatattc atatttattc tccagatgat
    14401 attgcaaata gccaggaaat ccaggatcga tctcatcccg actattgcca tgcccccgaa
    14461 tattcaaacc gaaactgata aaaccatctt cagtaatcag gttagtcggc tgcatattcg
    14521 taccatagcc ttgaacttga agaaaagccg ggtattttcc agggtttttc ggaaccgagt
    14581 accagccgcg aattaaaata tttcccagcg aacgcatttc aaccaggtaa taatcgcgct
    14641 cagcggtgca taattcatcc tggcgaatca tcttaaactg tgggtcaacc gtagccagtt
    14701 cggctttggc ttgctcccaa aaagcatcaa aatccggctg agcatcaacg ggcgaaatca
    14761 ccttctccgg atcaactgct atcgcaaatt tatgcaattt attccagcct aaactttcga
    14821 aactaaccgc cccgaggtaa aagccgggtt gcagtttctt taagttgtag gaatattttt
    14881 tagcagaatt atttttcatc gtaatcttta attctttatc atagatttta tccccgaaat
    14941 cactattgat cgccagcctg agaatacctt caattgactc attactttca ttacgcataa
    15001 taaccggaag tacaatttcg gagcaattct taatgacgtg atccttctga ggaagggcaa
    15061 tttcgatttt cagctgatcg gaaagaccaa tcacattcag ctcagcggct gagtagtaca
    15121 atccaccacc gccacctgcg tcataaactc gaattgcaat gacattcggc tgatcccagc
    15181 gaaccagttc gaccgggata atatattcgc gatccagacc ataagcaccg atataatttg
    15241 gtgggaaacc acccatctgc ccgattaatt caccattgaa ataggtagca tcggaatcgt
    15301 caatacgccc cagacgcaga ataaatccgc catgtttaac ggctttctcc ttcagcgttg
    15361 atggaataat tacactgtat ctataccagg caaatccatc taaattttca tagccttgca
    15421 cttcccaggt tctaccggca atgatagagt tccaggaagc gtcatcatat tgcggatcag
    15481 cccaggctaa atcatcgccg ggttgaaatt tccaatttgg ccctatcaaa ttctgagccg
    15541 ttaaaaccgt cgtaattgca atgcagacaa ttaatgacga tgtgattgtt ttaaaacgca
    15601 ttttatcccc ctttttaaac gttaaatttt atcgaaaaca tataaacatc attttgtaaa
    15661 taaaccgttt caaatataat taataaaggc tataaaatcc gagactaaat tttaaaactc
    15721 tcttaattaa tttttataac gcttgttacc agaatggtaa aaagctatct caaatctcaa
    15781 tctggagcga aaccggacaa tgatctgaac ccgctacatc ggctaaaatt tcggcggcac
    15841 gaacattcgc aacgaatcgt ttgtttacga aaaaatagtc gaggcgccat ccaacattac
    15901 gctctctagc gttggtgaaa ggactccacc aggtataatt acccggttca ttattaaata
    15961 atcgaaaagt atcataataa ccgtggctga gaaatttatc gagccaggct ctttcttcag
    16021 gcaaaaaacc ggaaatattg gaattcgcca ccggatgagc taggtctatt tcggtatgtg
    16081 ccgtgttgac atcgccacct atgatgatat tccgaccgct gacgtttacc cgatcaacat
    16141 aatcgagaaa agcgtcatag aattcgagtt taaaacgcag tcgctcctcc gacatttgcc
    16201 cattaggaaa ataaacatta aataaaacaa attgatcata atcggcaatt aggagacgcc
    16261 cctccgtatc gaatttctcg attccaaact tttgcgtaat ggaaaccggt tctaatcgag
    16321 aatacaaagc gactccgctg taaccttttc taattgccga cgaaaaataa gaacgatagc
    16381 cactgatttg ggctaaatcc ggcggtaatt gcgattcttc agcttttgtt tcttgaatcc
    16441 ctaaaatatc cggctgagta gctttgaccc aatctaaaaa acctttttta tgtattgccc
    16501 gaattccatt gacattccat gaaattaggg taagaagcat atcttagaaa accaaggatt
    16561 gatccaaaca gaaggcaaga ccttttccgt gattaaagaa tccgcccgtc tgcgcaaaac
    16621 cccattccaa acgatagtgc ttaaaagtca ttccactacc gacgccccat ttgagtttat
    16681 cattgcctcc gaatccaact ccaaacctta tgggtaacca atctgccggt aaaaattcag
    16741 ccgcgaaaga aaaagccgga gttgttgtgg atgccatatc attgctgaaa gactggcgat
    16801 aattgacaaa cagccgcaga ttttgcaata ctctatattc aaatcccatt atcatataag
    16861 ccgggtaatt tgtcctaata ccggaaactg tgtaggtagt gtcttttctt aaagattcat
    16921 ccattattga atctatatcg ccaaaatccg aactaggaat atcggcataa aaagaatatt
    16981 cggctttcct ggcttctcct atcccccaat ttaaaaaacc gaccacattg ttcaaggcaa
    17041 gattggcagt cattttttca ttaataacag acgccgctcc tatatcaatc gccagaccgg
    17101 caccgcccag tccgtattcg cctttgagat cgccttccag gataattcgg tctttatagg
    17161 tcgtaacatt cgcggttaga tcgtcgaatc cggaataacc cgctccagct aaaagtttaa
    17221 cggcaatacc gacggaaaat ttatcaacat aattactgag ataaggtaca ttatcaatct
    17281 gccatccgtg agctattccc agagtaacta ccgattgtaa atccagatca gtatcgctta
    17341 aatcaatagg ctcttcgaat tcattaccaa ataacgcaag acggaaaagc gacttgggtg
    17401 cgacaacttt gccggttact tcggcgccca gcgtaaatgc ccaactattt accgaaaaat
    17461 tgagaaaacg catctgcacc aagggattaa aattcaaacc gccatccgga aaatgactga
    17521 gtaattcttc cttagcatca tcatccaggt agtcaccggt gaaaatataa ttatttaata
    17581 aatgtgttga aatagtatta ttgctaatct gtaaagccgg aaatggcacg atcgggataa
    17641 ttccaaaatt gaattgaaat ttcggattat cgttcaatcc gagattagcc ggattccagc
    17701 cgatcacgtc accaccccgc gcttgtgtgg cgtaagcatc ggcaaaactg atactgcgcg
    17761 gattcatata gagctggcta taaccttgtg aaactataac taataaagcc ccaacaacga
    17821 tatattttat ctttttcatt agactaatcc tcctctttcc gatcagcaaa atcaatcaaa
    17881 ccattaatcg ttccgtaaag caatatcttt aattcatcgc ccttcataaa acgggaagga
    17941 atcggattcc ccatttcgtc agttcgtccg accagattga attgggtttt gacgtaaact
    18001 gagtcctgaa acaaagctat ttgcttttca cctaattcta caacttctcg aaatgatgaa
    18061 tcggggtgaa cattaatgac tgccagagta tccggaccat aaggtggagc aacatcgaat
    18121 aatagtgtat cctgagcggc taataagatc agtttaccgc caatctcgaa tccattctct
    18181 acttcggcat aaagaatggc gctttcaatt tcttctggaa tgtcttcttc caccaattcc
    18241 ggatcaaatt ccatcttggc atcttcagtt atttccattt caaacggtac agaaatggat
    18301 atattgccgc taacatattg ggctgtatca acttgaccag taccgtagac cagggcataa
    18361 ccggatgtaa cgattttctt cggtttgatg ttgattaatt cttctgcatc gggaataaca
    18421 acccggggat tctcggtaat gacttgatgg atttcccgac gaactgattc accttcatca
    18481 ttaaaggacg aaataaataa attgagttcg accggcacac caatatcggt attgaactct
    18541 attgccattt cgacattctt taagttgatt ccgtcgaatt cttccggtaa tgcattaact
    18601 tcattttcgg agggttcaat atccacttga accgtatcaa tatacccgct gacacttgca
    18661 aattctacct cagtcagtag aacgtcaaca ttgatttcct gcccaaaagt gattgtcatt
    18721 tctcgatcgg aaggaatact gactcgcgag gtgtagtgga tttcttggtt ggtggtcaaa
    18781 tccgtaaacg gcaattgaat attatattca tccagcggaa tggcctcaat caccggtttg
    18841 gaactgtttg gcaggtgaat aatacgttct aatggcgtac catctgtgcg atgcacaatt
    18901 tcattaattg taaaatgaac atcggcaaac actccgatgt tattaacaaa agtcaagatg
    18961 agattaccat tcgagatctg ggctgtctct aatttagtct tatcttccag ctcaagtacc
    19021 tcattagcaa cgatcgcttc ctggtcaaaa tatccggtaa caatcgaaaa agtcatttca
    19081 gatgtgttaa tatcaaattt gattgaatca attaaactat attcggctat ttctcctgag
    19141 gaagtcgtaa ccagactttt ataggtgata tactgccgaa cactgtcagc ggttacagat
    19201 tcggacaatg gttggattag acaattactt aagtcataat taatcgtagt ctgtccgcct
    19261 ggaatatctg attcgattgt tactggtgtt cccgcagtag ttaatatctc tggaaatgta
    19321 agaacaaggt gtggaattcc agttgtacca atgttaatgc cgttttcgat tgaaaaggtc
    19381 attccgccgg aggcaatttg cgccgtactg attcgggcat cggaagcggt attgatttca
    19441 ccgctatctt tgatgttttg cggctctaca atgcctttga tctccgaaaa agttacatca
    19501 gattctgaat tttctccata catatctatg ttgacattga cttgatcaga tgaagaaata
    19561 tcaaccattt cgggatcggt tgaaatggtt cgaactatat aagaatagtt gacttcttgc
    19621 tcgtcaatat ccatttctaa cttatggtca gttagcaaat attcctgaat tacagtctgg
    19681 ccagccgcca ttggaataac ttgataaaag tcactgccac cagaagcagt ttttaaagag
    19741 gggatggtga gttcgagatt agcgctgacc ggcaaatcat tctgaataga tattttcagg
    19801 tgaccatctt taattaccgc tgtttcaact ttattgggct cgtcttccgc aaattcaatc
    19861 gtgccagtag tgtcaatggt ctgttctgga acaatcgcct ccgccgatga tacagatatg
    19921 tccttagctt gaacttccac cacgaatgaa gaattcaaag tcgcagagtt tttagtgact
    19981 atttgcgaac ctgagccaca aatgactcca gaaacctcca cgatgattgt cccgggcaaa
    20041 gttttattgg ttagatttat tgttttttta ctggattgcc cggatgccaa accctcagtc
    20101 cagatggctt tagccgaatc tccgtccgtt ccgacaattg gatttccaga accatccagt
    20161 aagcggacat aaaccggtgc accaagttca actaccagat tattgcggat attaatttcg
    20221 aggaatcctc caataaaatt agcattatta aaattattaa aaacaatttg tcgatttatc
    20281 gtcggaaaag gtgtacttat ggcgatggtc ttgctggctc cgtccgctat gccggtcata
    20341 tccactatct ccgaaaataa aatcggagcc gtgctttcga catcggtatc agccagatga
    20401 atgtcaccga ttaggttgct attctgttca gaaaccggct caactccgac ttcatcaaaa
    20461 cccgaagagt attttttatc acttgcggca atattgactt catctacacc ttgctgaatg
    20521 gattgagtta tatcatcaat atttaattta ttaccgaccc gaacttcttc aattgggatt
    20581 tgatcttcat aggcaaaaat tgaatcgcca gcggtaccat aaggaagctt gactatcagc
    20641 gaatcttcta ataaatcatt gacggtaaca gtctcattta atagcgggac gttcagattt
    20701 accgaccagg tgggataatc cgattcactg gggattttaa gatcgcaaaa tgaaaataga
    20761 accgccactc cggcgatgat gatgatttta aacttttgca tgattatcct cattaaaatt
    20821 caaaattcct agttgatttt atagcaatta acaatagaat tatgtgacat aagtctcact
    20881 taaacgatcc tatgtaaata aacaacttac ctcttcgttt tttacaatgt tatataacta
    20941 ttcaagttta tgataaattt gctcaatttt attcgctatt ccaacggcgt ccagggctag
    21001 gtttctcagc aaaatttcac gcgctccttg ttcaataaaa cgatcgggaa tggctagaat
    21061 gttgatcttt tgcggcaagt tttgctcagc aaagaatctg gcaacctgtt ccccgaatcc
    21121 accgacaatg ctgttctctt caatcgtgat aataatgcga tgcttactgg caatctccga
    21181 caacatttga gtatcaagtg gtttcacaaa acgagcgtgg acaatagtcg gaaatatatt
    21241 ttttggttta agtaggcagg ccgcctctat cgcgttattg gtcattacgc cgaccgacaa
    21301 gatggcgacg tcttgaccat tttgaattat ctcccacttt ccaatttggt agggcacggc
    21361 tggtgcttga tcatcaaatt cgacataaga atcttttgga tagcgaatgg caaagggtgc
    21421 atcgctctgc tcgagtgcgg tgaacattaa attgcgcaat tcagtgccat tccgtggtgc
    21481 ggctacgatc atattgggca ctgtgcataa atagctcaag tcaaatgcgc catgatgggt
    21541 tggaccgtct tcgccgacga ttcctcctcg atccaggaca aatactaccg gtaaattttg
    21601 caaagccaca tcatggataa tattgtcgta agcgcgttgt aaaaaggtag aatagattgc
    21661 caccaccggt cggaatccgc aagccgctaa accggcggca aaggttaccg catgcccttc
    21721 cgcaatcccg acatcgaagt aacgcattgg aaagcgtcgt gcaaattcga ctaatcctgt
    21781 gccttccgcc attgcggcag tgataaagat agacttggga tatttttcac caatttcaca
    21841 ggcgatttta ctgaaagcat ttaaaaaggg cggagcggtt ggtgttgaga cacaagcatt
    21901 aacatctttc cgcggaccaa ttccgtgata tttaatcgga tcaatttcag cgctggccag
    21961 ccccttccct ttttgagtaa taatgtgtag aatgacaggg tatttcaagt ctttgatatt
    22021 attcaaagta ttaacgagtt gctgaatatt atgaccgtca accggcccgt aatagcgaaa
    22081 tccgaattcc tcaaagatta tcccgggcgc aagaaaattt ttgaatgact ccaataattt
    22141 ccgccctgcc ttccgaaaaa aattggtgcc gcggggtaat agcgccagtg aattccagac
    22201 ttggttttta aaattcaggt aactaggact ggttacaatg cgggtaaaat aattagacat
    22261 tgccccgaca ttacgtgaaa tcgacatctc attatcattc agaatcacta agaattggcc
    22321 gttaatatta taagcgttat tcagaccttc ataagccaat ccaccggtaa atgctccatc
    22381 tcctattata gccgcaattc gatagttttc atgattcaaa tcacgagcaa ccgccattcc
    22441 aaccgccgcg gaaatcgagg tgctagcgtg tccggccccg aaacagtcat attcgctttc
    22501 ggacatttta caaaatccgc taataccacc atattggcga atagtcttaa tgccttctct
    22561 acgaccggtc aatattttat ggccatacgc ctggtgaccg acatcccaga ttaatttgtc
    22621 agtcggcgta ttgaaaacat aatgcagagc aatcgtcaaa tccacagtcc ccaaactcga
    22681 tgccagatgg ccaccggtat tggtaacggt ttctataata tattcacgta gttccgtact
    22741 taaactaacc agctcgtcaa ctgataattt tcgcaaatca gccggagagc tgacttcctc
    22801 caatcgggga tattttttcg cttcttgttc cgccatcatt gtaagtgatt gagaaaatat
    22861 tgatgaatac gaaaatcgtc agtcaattcc ggatgaaaag ttgccatgag attattgcct
    22921 gaccgggcga gaaccacatc gccatttaaa tggccaagaa tttcaacaga agaatcgaga
    22981 tgatcgagtt tcggagcgcg aatgaaaatg cctttgaagg gtttgtcgga aaacgtaaga
    23041 tcaacatttt caacaaatga ttctatctgt cgtccgtagg catttcgctt aactttaacc
    23101 gaactacgtc ctaatgattt gaccaatgga tccgtgccgt ttgttgatag caaaattgct
    23161 ccggcgcagg taccaaaaat catcccggga aatttctcaa gactttcgcg caaatccatt
    23221 tcgtcaatta gccttgacat cgtggttgat tcgccaccgg gaattatcaa accgtcacat
    23281 ttttcgattt gttctttata gcgaataacg atggcttcgg ctccgaggga attgataatt
    23341 ttgaggtgtt tctgaaaagc gccctgaagt cccaatacgc caactctttt ggctttatta
    23401 ttcataagat agtgagattc aatatactat ttctattttt accaaccgcg ttttgcgagt
    23461 cgttcatttt ccggaatctc ggcgagatcg agcccacgca tagcttctcc caatccggcc
    23521 gaaacttcag cgattttttc gggattatta taataagttg tcgctaaaac gatagctcga
    23581 gcccgggcgg ctgggtcttc ggatttgaaa attccagaac ccacgaaaac actctccgct
    23641 cccaattgca tcattaaaga agcatctgct ggtgtagcga ttcctccagc cgcaaaattc
    23701 ggcactggta atttgccggt tttagcaatc tgcctaacca attcaaaggg agcgcccaga
    23761 cgtttggctt ccgccattaa ttcatcatcc gacaaagtcg tcagtttacg aatttcggat
    23821 tgcacccgac gcatatggcg caccgcctcg acgatattgc cactaccggc ttcgcctttg
    23881 gtgcgaatca aagccgctcc ttcgccaatg cggcgcaatg cttcacctag atcgcgacaa
    23941 ccgcaaacga acggcacctt gaaatcgtgc ttccaaatgt ggttttcttc gtcagccgga
    24001 gtcaaaactt cactctcatc aataaaatca atccccagcg cttccagtac ctgcgcttcc
    24061 gcaaaatggc caatgcgaca tttcgccatc accgggattg agactgtatc ctgaatcgct
    24121 ttaattactt tgggattcga catacgggca attccccctg ccgctcgaat atcggctgga
    24181 atcctttcga gtgccatcac agcgacggct cccgcctctt cggcaatctt ggcttgctcg
    24241 ggcgttgtta catccattat cacgccgcct tttagcattt cagctaagcc aacttttact
    24301 tcaaaagaac ctttatccat tttgaataat tactcctttt catacgatta aatttaagac
    24361 atttcagtca aaattggtta tttttttaac cacctcttcc accacttcaa caggcgtcga
    24421 ggctccagtc gccacgccga ctgatgtctt attaataaac cagtccggat taatttgctc
    24481 ggcattttca atgtggtaag tattggtatt caactttttg gcaatttctg ccagcctccg
    24541 tgtgttggca ctattttttg aacccacaac tagcataacg tcattggtta aagcgagttc
    24601 ccgaatttgt tcctgacgtt gacgagttgg acggcagata gtattcataa agcgcaggtc
    24661 gtcggtttta gccgccagaa tcgcgacgat tgccgataca ttttcaatca tctgagtgga
    24721 ttgcgctaca accccaattt ttttcatctt tggtaaacgt tcggcctcct ccggactcga
    24781 aacgatgata ggatcgttga tttgactggc aatgccgctg acttcttcgt gatttttatc
    24841 tccgataatt atcacctgac ggccttcttt ggctaacatt tggacttcgt tgtgaatttt
    24901 acgaactaga ggacaggtcg catcaataat tctcaagcct cgttgatgcg cttcatccca
    24961 aatggatcga ggcgtaccgt gcgagcgaaa taaaacgggt acggttggtt cgatctggtc
    25021 aaggttttgt actacttcga cacccaaatc ggttaattca cgaatcactt tttcattgtg
    25081 aacaatgtca ccaagcatcg ccacccgacc aaatttttga gccgcggttt gcgccttctt
    25141 tactgcgttt tgcactccgg cacaaaaacc gaccgtagcc gagatgttga cttttaaagc
    25201 cattattcct tgatcttcgc gttcgtaatg ttttgctggg cttggtattt tcctttttta
    25261 tcggcgtacg aaatccgaca agcctcgtcg ctttccaaaa aaataacctg cgcgacacct
    25321 tcgttggcat agattttcac cggtaccggt gcgagattgg caatagaaat ggtaacaaaa
    25381 ccctcccatt ccggctcaag cggggttaca ttaacgaaaa tcccgcacct agcataagtt
    25441 gacttgccgc tcgtaacggc aatgacattg cgcggtatcc taaaatattc cagactacgt
    25501 ccgagtacaa aactattcgg cggaatcgtg caaactgcac ctttgtaatc aataaataaa
    25561 tccgtcgtta tttgtttggg atccagaact gtatcctttc gaccgtgaaa aatacgaaat
    25621 tcatccgcca ctcgcatatc atagccgtag gaagatacac cgaaggaaat tactcctgaa
    25681 ctaatctgac ttttttcgaa tggctcgatc attttatgtt tcaaagccat ttcctcgatc
    25741 cagcgatcgg atttgacgct cattttatcc ttcatccaga aaaacttttc gcaaagccgg
    25801 taaaactgtt tttacggcgt cttctaatcc aacatccatc accagaccgg ccgcataagg
    25861 atgcccaccg ccaccaaact gacgcgcaac attattgatt ggataatgcc ctttggaacg
    25921 gaaattcatc cgcgtgcggt tatcacgctc ttccagaaac atagcggcaa tttcgacacc
    25981 ccggattgag cggacaacat ccgtaaaacc atcaacttca tctaaatagg ctccggcggc
    26041 ggctacctga gcccgcgtta cgcaaaacca tgccaaccga ttttcaaatt catattgaac
    26101 attctgcaat accatcccga gcaatttcat cctttccgga cgatagttct cgtaaacata
    26161 gtgataaatc aatcccggtt gaacgttgta ccgcaaaagt tcagccgcta atagcatcgt
    26221 ttcagcagta gtattttcaa atcgaaatga accagtatcg gtcattaagc cgacgtacaa
    26281 agcctcagcc actttttgat ctattgcttg cggcgctatg atttttaata attcaaaaac
    26341 cagtgcgact gttgccgggg ctttatcatc aatgacgcta atattttccg gcaggcagtt
    26401 attaaacgga tgatgatcaa tgcaaatagt cggaatatta agtgatttca gaaccggacc
    26461 gagcgccccg agtcgttccg tatggctggc atccagaatc attaacaaat cgaaatcagt
    26521 tagttcaact tgatctaact cgttggggta agtatgaaaa atttcaccct cattcagaaa
    26581 tgaatactcc ggcggcaaag gcgatgaatt caagataaat ggcgacttac caagatgctt
    26641 gaggcaatgg taaagcgccg cttctgcacc cagcccgtcg ccatccggat tgtggtgagt
    26701 ggtgagcagg attttatccg actccttgat tttctctacg attttttgcc agttatattt
    26761 ctccattatt accttttaaa cgaaccttta atttagtttt tatatgccaa ttgtcaaatc
    26821 agagtgtaga gtaaaagtgg attatatcct cgaattagat aaatcctgat taaattctta
    26881 gaacaactat taatcaataa tctgaatatt ctttatccca ctgggaataa cttttcgatt
    26941 tcttctacaa tacgtcaacc gcagagcagt aaaaaaaggt aaaagatttt gtcccgccga
    27001 gcgggactct tccggagtca atcctgtcta aaatattcaa ccgctccatt tatcatcatt
    27061 agaaaattca gtcgcatttt aaaataaaat cgtaaatttg tgtaacaata atcctatgag
    27121 tttatcaaaa taaaaaaata ggagaaaaaa tatgacgaag cgaactcaaa tttacaaatg
    27181 tgaaatctgc ggcaatatag tcgaggtttt acacgaaggn nnnnnnnnnn nnnnnnnnnn
    27241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27301 nnnnnnnnnn nnnnnnnnnc tcaaatttac aaatgtgaaa tctgcggcaa tatagtcgag
    27361 gttttacacg aaggcgcggg cgaattggtt tgctgtggca agccgatgaa acttatcgta
    27421 gaaaatacga ctgacgccgc ccgagaaaaa catgtcccgg taattgaaaa aatcgccgac
    27481 ggctacaagg tcagtgtcgg tagcgttctc cacccaatga ttgacgttca ttatattgag
    27541 tggattgaat tggatgccga cggtcaggtt tttcggaaat atctgaagcc gggcgataca
    27601 ccggtggcta tttttaacgt atcggccgaa aatgtaaccg cacgtgagta ttgcaatatt
    27661 cacggcttat ggaaaggaac attataatgc ttagtcccaa attattaacc gctttcaatg
    27721 atcaaatcaa acacgaactg gaatccgcct atctttatgc cgcaatgaat gcctggttca
    27781 aatcggaaaa tttcgacgga atggcgcatt ggatggaagt tcagaccaaa gaggaaataa
    27841 accatgccag aaaatttttc gaccatattt tcgagcgtgg tgatgtcgtc gaactccagc
    27901 caataaatat tttggctaag aaatgggctt cgccgctcga cgctttcaaa gccgcttatg
    27961 agcacgaaca atttgttacc ggcaaaatca atgatctaat gacgatggct attgccgata
    28021 aagattatgc cgcacaggaa tttttaaatt ggttcgtcgc tgaacaggtc gaagaagaag
    28081 attcaacttt gaaaatagtt caacttttaa agagaatcgg cgatcacggt caggctttga
    28141 ttatggttga tcgagaactt ggtaagcgcg aataatagga aagagccaat tatgaatttt
    28201 aacatagacg aagttttcga aattgccgag cagatagaag tcaatggcag ccgtttttat
    28261 cgtcaagccg ctgaactcag ccagctggct gaacataaaa atatgttatt aaagttggct
    28321 gagatggaag atcagcacca aaaaacattc gctggcatgc gtgaaaaaat aaaacaaacc
    28381 aagcaaaatt tgtcttatga tcccaatcag gaagccgctc tctttttaca agcctttgct
    28441 tctggatacg tgtttgatcc aacggctgat tcatcggcgt taatcagaga tcaaaaatca
    28501 attgcggata ttttgcactt agccattgga ttggaaaagg attcaatcgt tttctatctc
    28561 ggcatcgaaa aaatcctgct caacgaagac gatatcgttc acgtacgtgc tattattaaa
    28621 gaagagatgg gacatattac tctgcttgcc cgaaaattag acgaactgaa ttaagctaaa
    28681 cggcaatttg ttttgattta atacccttac aggtatgatt acttgtgagg gtattttttt
    28741 ataatattct tgatgaaatt aatgcaggct gtttaaattg acgataatga ttaacactaa
    28801 aataattaaa ggaataaaaa atggcaattc gtatttttgt aacgggcggg acttttgaca
    28861 aagaatataa tgaactcacc ggacaactct atttcaaaga cacgcactta ccggaaatgc
    28921 taatcctcgg gcgctcgcgg gtagaagtcg aaatccgcac tttgatgatg attgatagtc
    28981 tggaaatgac cgatgatgac cggctattga ttgctcacca ttgtcagaaa gccgctgaag
    29041 atcggattgt cattactcat ggcacggata caatggtaga aaccgcccgt ctcattgccc
    29101 aaaatgtcag cggcaaaact atcgtcttga ccggcgcgat gattccttat aaatttggta
    29161 gttcggatgg actcttcaat ctcggtagtg ctttagcttt tgttcagaca ctttctcccg
    29221 gcgtttacat tgcgatgaat ggtcgctttt ttag
//
LOCUS       DAOMJU010000018        28584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1480_length_28584_cov_21.033124, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000018 DAOMJU010000000
VERSION     DAOMJU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 28584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28584
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1480_length_28584_cov_21.033124"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..248
                     /locus_tag="PLO58_03025"
     CDS             114..248
                     /locus_tag="PLO58_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73794.1"
                     /translation="MDNYESAITALKIPFFPLGLRSPAGDVLNAVSKLNGQILGSPFY
                     "
     gene            complement(342..1772)
                     /locus_tag="PLO58_03030"
     CDS             complement(342..1772)
                     /locus_tag="PLO58_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HQO73795.1"
                     /translation="MYLLIAILLIAAIYSTKITSKFGVPVLLLFLGIGMLCGSDVLNL
                     IYFDNAVLAQKVANIFLIFILFEGGFHTRREIVQSVFGPALTLATFGIALTAGILGLL
                     IHFIMGLDLLYSLLIGSIISSTDAAAVFMIMRQRAIKKRVSLTLEVESATNDPMAIML
                     TIVFIQIITGHPQNIGLFILNLIWQFGGGIVVGWLISKVGAFLFNNLKVENRGYYHVL
                     SIAVCLLAYGSAEVVKANGIIAVFFAGYWLGNTDFAYRKGVGNFIEGISTFSNIAIFL
                     LLGVLVFPKSLLSVWQEGLLITAIMILVARPLTILICTLPFNFKFREKVFLMWGGIKG
                     AVPIVLATYPAVYGIDQNHFIFNIVFFAVFLSCLLQGVTLTWGASKLRLCIPSRPKPI
                     HSIELITTRKTDVDVFEIQLSDNSKIDGLQIRDLALPPDSLIIAIIRDDQIIPPKGHT
                     VLKKGDILFVIAAQDNVERISEILSK"
     gene            2266..2589
                     /locus_tag="PLO58_03035"
     CDS             2266..2589
                     /locus_tag="PLO58_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73796.1"
                     /translation="MKEILQVIIDEEKSARERIENARLQARRDTEAAQIEAQKTIETA
                     RQLALQEAEALITTAKSEAECQHAELLNAARAQQNDIFNKYHAEIAAAVDFLFQTLPI
                     GKKVA"
     gene            2589..3620
                     /locus_tag="PLO58_03040"
     CDS             2589..3620
                     /locus_tag="PLO58_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HQO73797.1"
                     /translation="MSAVSTYGLVNARVRTRRSALLSASSYKALAAATDLPEMLNLLT
                     RYKNRLSFENLPDYSIEALERLLIQEKARQILDLKYSSRGPARDIMALMLSRIDAENV
                     KTILRAWQKSDQNVSNIIECTGLAVLPITKMLSAEDLTQFAAYINQLPFYEAFLKAAK
                     GFDRSKSLFEIEVAIDKSLYQSLWDLTALLNKTDQHIVRRMVGIEIDLKNLDWIARIR
                     KYYQSQIKVTETSLIPNGYRLPYATLRKMITTGSIEDGLEKILPGLTPITAQERDQLT
                     ALERLEQFLNQALFHEANRLFLENPLSIASTIGYYYLVHIETRNLCTLINAKYYKLAP
                     QAVLNKLVY"
     gene            3701..5593
                     /locus_tag="PLO58_03045"
     CDS             3701..5593
                     /locus_tag="PLO58_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HQO73798.1"
                     /translation="MIIHQGDLQIVDAAQTNPWIKDLARTYSTEESSQSQARREKVEG
                     LVKTLKLTDQLHNFQPLSGDWMEIDQSLETAIAEIESVSQRRDSLQKEFQRLNEIKTR
                     YDRFPGLGKALQMPGDYSYLAIETGEIPDNNLPLLEEQLSATLHVILPIGRQKGMTSL
                     VAIVLKRDAEVLQAALKASGFQPTKIEKELPSLATESIEKLEKELNDLNEQLRDAEEK
                     FQAVVNRHRALLIGVYLRIRQEQVQGRMLHFFRRTEKTCLFSGWLPDSRKEVFVKELQ
                     KATQNRAIVDLIPAESHEAVKDGSVEVPVKLNNPAFFKPFEMLTSVYGLPKYQTIDPT
                     PVFGLSFLLMFGMMFGDIGHGLVLALLGLALLLKSHKDTFKSAGYLLLYAGGSSIIFG
                     FLFGSFFGIEDLFPTIWMKPMNDINQLFKVVIYVGAGMITVSIIINLINGIRSGRILD
                     YIFDKAGLLSGILYWCGIILVSRLVLTKTNGGVGLFVIYLFVACALLLFFREPILNLI
                     RKNRRLYSSGVVTGLMHSLVEMLEIVLGFLANTVSFIRVGAFGLAHAGLFIAIFALSD
                     MVSGKLSGVASILILILGNIFIIALEGLVVTIQAIRLEFYEFFNRFFRPARVKYQPIR
                     ENPNLE"
     gene            5641..6042
                     /locus_tag="PLO58_03050"
     CDS             5641..6042
                     /locus_tag="PLO58_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HQO73799.1"
                     /translation="MLKIKSFKKLLAIVITLGFVAIGGTSLLVLKAIAQTPEETATSV
                     VESTETTVEVAPNTARDSDTIKFGFIAAALAVGIGSIGAAIAVGNIGTAAMGTIGEKP
                     ELSGSALIFVGLAEGIAIYGLIVAIMILGKL"
     gene            6058..6366
                     /locus_tag="PLO58_03055"
     CDS             6058..6366
                     /locus_tag="PLO58_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HQO73800.1"
                     /translation="MKYFVIGDEETVLGFSLAGADGIVVHDAASARRALAGTMEREDI
                     GIIIITERVATMIREEVNAYFYHSPYPLIVEIPDRLGPLEDRISVRDVVQTAVGVRLE
                     "
     gene            6373..6996
                     /locus_tag="PLO58_03060"
     CDS             6373..6996
                     /locus_tag="PLO58_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HQO73801.1"
                     /translation="MPDKNATLEAIVAEIRQMSDAEIEAIRQSASREQAQIAQRARQE
                     AEKIRAGIISKAQQNAEKIRQRSAAQTNLEIKKLNLQSRLLITAEIRRQFEERLFALR
                     QSPEYAQLLKELIVEGIVGLGVDHILISAGDREHTILTDEFLRSIEASFNPPVHLELE
                     PVVLNEPGVILYSADKRRRFDNRLSRYVQRVLEKYHWEIMQKFSENN"
     gene            7009..8775
                     /locus_tag="PLO58_03065"
     CDS             7009..8775
                     /locus_tag="PLO58_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HQO73802.1"
                     /translation="MAIVIGKVTRVLGPVVHAIATAKVQMLEFVEVGTERLIGEVVRL
                     HGNQIIIQVYEDTTGLTPGVEVYGSGMPLSVELGPGLIGTIYDGVQRPLTEIKKVSDQ
                     YIHRGIKAPALDRNKKWSFEPAVSPGTIVSSGQIIGSVKETVSITQKTLVPPNISGKV
                     ISIAPPGEYRIENEICVIENGQIRHTLSLFQRWPVRQPRPIKNRLSTSQPIITGQRVI
                     DTLFPIAKGGTVAIPGGFGTGKTMVEHQIAKWSDADVVVYIGCGERGNEMTDVLTEFP
                     QLIDPRTGRPLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYHVAVMADSTS
                     RWAEALRELSGRMEEMPAEEGFPAYLPTRLAEFYERAGYFETLNQETGSVTIVGAISP
                     PGGDFSEPVTQHTKRFVRNFLALDRDLANARHYPAISWLDSYSDYVEMVTAWWQEKAD
                     PDWAELRQEMLDLLQKEAKLQQVVKLVGADVLPESQRLILEVCTLFKNVFLQQSAYDQ
                     IDTYSTVQKQAKMLRIIVTFYRLSADVLRRGATLYKVKRTKTFRAIMNMKYEISNDEI
                     DKLDQILANLTQELASLSRPNF"
     gene            8788..10230
                     /locus_tag="PLO58_03070"
     CDS             8788..10230
                     /locus_tag="PLO58_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HQO73803.1"
                     /translation="MLQIKKINELNKYNQAELLKYREYIGIQEVAGPLICIENIHNVG
                     YNELVEIRDERGEPRIGQVLEANQSAAVVQVFEGTTGLSLAGTAVRFHGTPLRIPVTK
                     EMFGRIFDGLGRPIDGGPEPLTNEFWDANGESINPTARTYPTTFIQTGISAIDGMNTL
                     VNGQKLPLFSGPGLSHDRLTAQIVRQAKIVGREVSFAIVFAAMGIKHDIAQYFIDNFR
                     DAGVLENVVMFLSLADAPSVERLITPRSALTMAEYLAFHENMQVLVVMTDMTNYCEAL
                     REISIARGEIPSRKSYPGYLYSDLASLYERAGMVDNSTGSITQVPILSMPNDDISHPI
                     PDLTGYITEGQIVLDRELFQQGIYPPIALLPSLSRLMKDGIGEGLTRADHAHLASQLF
                     AAYARVKEVRALASVIGEEELSAVDHSYLKFGLAMENKFIAQKETEDRSIYETLDLGW
                     EMLSLLPREELQRITEEEAQQYHHYQNENK"
     gene            10243..10878
                     /locus_tag="PLO58_03075"
     CDS             10243..10878
                     /locus_tag="PLO58_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HQO73804.1"
                     /translation="MATRIKLVPTKSNLLKLKRDLSFAQEGYELLEQKRQVLVSQLMA
                     MVNEAIVAHEKMDTALREAYSALQNAIISDGKSTVKSMARAVRIKSDIQIYNLRVMGV
                     TIPKVEVEIEDDAPYYSAGNSSFWVDESIFRFKQILEVMGRFAELRVSLLRLAQEVRK
                     TQRRVNALEKIAIPDYQETIKFISDSLEESERGTLTSMKYIKEHLEKRKDA"
     gene            10871..11374
                     /locus_tag="PLO58_03080"
     CDS             10871..11374
                     /locus_tag="PLO58_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQO73805.1"
                     /translation="MPNTQSGRLISKILLLTDGSEGCISVAKYGIILSKITGAALKAI
                     YVVNTDLLKQLERARIFVKIEEVDYESDLEDDGQDYLQDITELAASKGVHLETELLKG
                     IVHKIAIQKIEEWNADLLIMGELEPGLPRQDISHDELGLLLRKARCSVLVAKEQRGLD
                     ELFETLT"
     gene            11410..11865
                     /locus_tag="PLO58_03085"
     CDS             11410..11865
                     /locus_tag="PLO58_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HQO73806.1"
                     /translation="MKLSNLIKREAIVVPIQNTEKQLVIEELLEAAAATGQILDRAKA
                     LQALLDRESLGSTGLEKGLAIPHARTNAVDGLVISLGVAPDGIDFQAADGGLSYLFFL
                     LLASDALTSTYVQALALIARLYQQDDFRREILNATTPQEIIAIIRKAEE"
     gene            11948..>13047
                     /locus_tag="PLO58_03090"
     CDS             11948..>13047
                     /locus_tag="PLO58_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HQO73807.1"
                     /translation="MNTIEKALMHGLFVHLHHNIDKVIDFKSFQELNRQVAKVWPGAL
                     LIGPEANDDAAVFQIPGMPGYVVGKMESHCSPCVPRPYDAAATGAGGAMRDVVAMGAR
                     PIFLMDFIGTRPLEQEVIVGPCGFSGRCTCGKCKVMTSQERVNIMVKGLGDMCRTMDV
                     FIIGGGFSTSFSDIVPAVVVSVIGKLVTEKPLTKPAKNVGDKVIIIGVTGNDGNDTLF
                     RAGLVEEMRPAIALFKEEKTAMEAALAAFATGKINACSDLGAAGIGAAICESARFGRR
                     GAKVDLAKVPVSAKDITPQEILICETQARYVVQVAPESVDEVLKAIRTKTEHVAVIGE
                     ITDTQQEVFEYKGEIIATIPNQPSPETLAILK"
     assembly_gap    13048..13147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13148..14491
                     /locus_tag="PLO58_03095"
     CDS             <13148..14491
                     /locus_tag="PLO58_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQO73808.1"
                     /translation="TSSNTMENINKNKQVVKIVNEPDLEYQIKIYSRNAKIVDDSWDF
                     RNSNTKDFTHCFHSYPAMMIPQVARRIIDNLGKKSKILFDPYCGTGTSLVEANLKGID
                     AIGTDLNPLARLIATAKTTKIDIKILDLFLQDFKDYIFSIDFDIGKIYSEIIPDINNI
                     DYWFSKVTQQKLSLILKYIDNISEFSIKNFFKVAFSETVRESSYVKPGEFKLVRSKNY
                     LDKSEPDVFRMMLSKLLRNRIGLIEFIECCKNNAKTSVYDFNTINEIPNAILPTEIVD
                     IVVTSPPYGDSRTTVAYGQYSRLANEWLGFKDANKIDRILMGGEKKKHIPVFKSGILN
                     DVIDTIQKEDKERALDVISFYEDYEKSIKNVSATIKKGGFACYVVGNRTVKGLTIPND
                     EITAQLFEENNFSHIETIVRNIPNKRMPLKNSPSNVVGRTASTMKNEYIVIMAKN"
     gene            14493..>14708
                     /locus_tag="PLO58_03100"
     CDS             14493..>14708
                     /locus_tag="PLO58_03100"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HQO73809.1"
                     /translation="MQLKELIKKLEALEQKGYLETSRQGPTGIGHLLEKEMGLFENNI
                     PIPDIGGRVELKATRRNVNSLITLFTFN"
     assembly_gap    14709..14808
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14809..15297
                     /locus_tag="PLO58_03105"
     CDS             <14809..15297
                     /locus_tag="PLO58_03105"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HQO73810.1"
                     /translation="NKGVWLVKQNNIIEKYGYKDDRGRQALYNIVNKKTPNSQGFYLI
                     SDPERHLIILKNIYEDVNIAEWSTYVIAGKFMTKLDRLLLIIADNKIENGKEYFHFNE
                     AYLLENPTPERFLLAFEKNELMIDLRMHLKPSGGVRNHGTGFRISEKNLIDLYEKKKR
                     LI"
     gene            complement(15337..17109)
                     /gene="aspS"
                     /locus_tag="PLO58_03110"
     CDS             complement(15337..17109)
                     /gene="aspS"
                     /locus_tag="PLO58_03110"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQO73811.1"
                     /translation="MLKRTLTCGDPRVEHVEQIVILNGWVNRRRDHGGLIFVDLRDRY
                     GLTQVVFDPQSGEEIMTLARKLGLEDVIAVRGKIRRRPENAINREMATGAVELLAIEL
                     EILNEARPQPFLVTDRKTGLEDLRLKYRYLDLRTSELQRNIAIRHKTYQSVRNFYYEH
                     NFYEIETPFLTRSTPEGARDFLVPSRLCQGRFYALPQSPQQYKQILMVSGFDRYFQIV
                     RCFRDEDLRADRQPEFTQIDVEMSFVEEEDVLQSTEEMLIRVFREVAGINLESPFPRI
                     PYDEAMGQYGSDKPDLRFGYEIHTLDKIVERGEFEVFNKALASGGTVAGICFPQGASI
                     TRKLIDELTEQVKKMGVQGLAFTRVTANGLEGGIARFLTPVAREIIAEFDANDGDIIF
                     FSADSREKTYSALGVLRLEIAKRLQLIPANVFKPVWVTRFPLLEWSAEENRLVAMHHP
                     FTAAVPEEAELLATNPAQVHARAYDIVINGHEIGGGSIRNHTVEMQKRMFDVLQINPE
                     TAQEKFGFLLEALSYGAPPHGGIALGFDRLVMVLASAENLREVIAFPKTTSGASLMDG
                     SPAPVEEQQLKELGLQLIKDKSSF"
     gene            complement(17169..17357)
                     /locus_tag="PLO58_03115"
     CDS             complement(17169..17357)
                     /locus_tag="PLO58_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73812.1"
                     /translation="MNHKLLYEIFRSDQCQFNAYQVGRLGLSDDHQPAFSDDYVLDIF
                     SVITIKSYRFSRNHFSAG"
     gene            complement(17434..17847)
                     /locus_tag="PLO58_03120"
     CDS             complement(17434..17847)
                     /locus_tag="PLO58_03120"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQO73813.1"
                     /translation="MSAIIKKKESSINTIIGEKTIITGSLRLEGDIIIYGKISGDIET
                     DGTITIFPGAEIKGNLQSANLNIGGHVEGDVIATGKVTLGDRSFLRGDIKAAQIVIED
                     GAVFEGHCEMHFPYPLEAQSPNAAKPAADDANLPQ"
     gene            complement(17849..18658)
                     /locus_tag="PLO58_03125"
     CDS             complement(17849..18658)
                     /locus_tag="PLO58_03125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQO73814.1"
                     /translation="MKRKYTLHLLSESRAKPIIWTFSHTTARLIIIGSLAFLALLIVT
                     ILITLPKAIKYRQVTLENAQLVQDRLKVSQILSDYNEIRQMDQYIRSVLGTDLVLTPS
                     DSVSGEAVNYLKNDPDQNRLVQISFLENIPIYPPVDGYITRGFIDDKVMTSENHYGVD
                     VAAAEGTPIKAAAAGVVVFSNWTYDLGYTVILYHSDGYFSIYGHNQRNSVVAHQYVKR
                     GEVIGYLGNTGKSQGPHLHFEIWREGVPINPQELIYSYKKADISLKYEGEY"
     gene            complement(18655..19527)
                     /locus_tag="PLO58_03130"
     CDS             complement(18655..19527)
                     /locus_tag="PLO58_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQO73815.1"
                     /translation="MTTSKRLGKGISALIPEFPEGIEQVYTMAEIEVAQIKFNPHQPR
                     QEFGAQAMAELIMSIREKGVIQPITVRQVDGGFELIAGERRLRACQELGKTTIPAYIM
                     PVATEAEMLELALIENVQREDLNPVDEARAYQILASTFNLSHEEIARKVGKERPTITN
                     SLRLLNLPDAILADLRSGELSAGHARPLLSIINPQQQINLWQKIKREGLSVREVEKLS
                     KKFQNIPLKEKPPLPVKTPTTKAIEDRLIHILGTKVKIKGDENKGQITIEFYSREDLA
                     RLLEIFKIIEEKTL"
     gene            complement(19502..20278)
                     /locus_tag="PLO58_03135"
     CDS             complement(19502..20278)
                     /locus_tag="PLO58_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQO73816.1"
                     /translation="MAKIITVANQKGGVGKTTTAVNVAAGLAVSEIPTLLIDMDPQSN
                     ATIGCGIDYRNLENTVYEVLIEGFDINTAIMPSEIPYLDILPASTRLVGAEIEMVSLI
                     GRELVLKKSLELLTRDYKYVIIDCPPSLGLLTINSLAASDSVLIPIQCEYYAMEGLGQ
                     LLNTIRLVQRSLNRNLQIEGVLITMYDSRLRLSHEVSEQVRSHFKGKVYKTVVRRNVR
                     LGESPSFGKPIILYDATSAGAQDYMNLVGELLNDDKQTAR"
     gene            complement(20421..21869)
                     /locus_tag="PLO58_03140"
     CDS             complement(20421..21869)
                     /locus_tag="PLO58_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HQO73817.1"
                     /translation="MKIRNIIAVLGSLFTILGLFLILPLLISLFQHDAATPAFLFSIA
                     VATGVGLLMQIRKPRNFNIQRWEAMVIVVIGWIGASVIGALPFYFSGYFDGFINALFE
                     SVSGFTTTGASILNDIESLPISLLVWRTLTHWIGGMGIIILALVVLPAINISGRNLYS
                     FESSSLANEQITPKIKDTARLLWTVYFILTVSETVLLLILGLPFFDAALTAMATIATG
                     GFSPKNASIAAYNNVYVEIVVVIFMILSGINFTLYLRMFQSRKNWRDYSETVYIYFAI
                     MAVASLVIVINLRSHTYNSWSEAIRYGVFQAVSINTTTGFVTTDWEIWQPVTHIVLLI
                     LMLIGACPGSTCGAIKNSRIILLIKSIHRELIQIIHPRVVTTIHFNKRPIDEKTIQNA
                     IVYIGMYLCFWLVASMIVALGGVAPESALIAVATTLGGVGPGIGLFGPIESYAALSVL
                     NKIVLMASMLFGRLEFYSILIVFLPTFWQRAH"
     gene            22060..22788
                     /locus_tag="PLO58_03145"
     CDS             22060..22788
                     /locus_tag="PLO58_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQO73818.1"
                     /translation="MQNLIYVGRWLVGLVYFLLLGMTLRFGLIFWKPAEIYVLVQQIC
                     RAALRTIGIRIKVSGLDRFDHQQSYLIICNHESLLDAFICPAYIPMYFTTIELADHFK
                     WPLWGWMIRNWGNIPLQHGQLKSSLTSLDQAATLLKNGTSILIFPEGSRTTDGKLLSF
                     KKGAFYLAYRAKADILPLAINGLWRAKTRGDWRLRSANVSLNFGEPIRYSTYQHLSPD
                     ELRNLCFETIQQLKSPVASNVLSS"
     gene            22839..23618
                     /locus_tag="PLO58_03150"
     CDS             22839..23618
                     /locus_tag="PLO58_03150"
                     /inference="COORDINATES: protein motif:HMM:NF019003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HQO73819.1"
                     /translation="MRKTDFSPLPERKQLLYEQMARSYRTQERQKNTPWAPFEKRLIE
                     SKIAVISIAGAYLNGQKPFTNRETESDYQCREIPLNFNREDLRFFATDWEPSEALADY
                     NVVLPIDRLVLLQKEGMIGKIHETIISIAGFTKSIKMFGKFLTKTYDTLKKAEVDGVL
                     ILPCSAQTSAAACQVANYLEKKELPTVILTTFYEQALALAPPRCAFINFPFGRPFGKA
                     NHVTLHTAILRDTLRLFEKAKTPGEVICLSYIWSFGTVPNW"
     gene            23637..24254
                     /locus_tag="PLO58_03155"
     CDS             23637..24254
                     /locus_tag="PLO58_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQO73820.1"
                     /translation="MNLLDNLTWLGHSSFRIAGKKIIYIDPFQVKAGIPADLILVTHD
                     HYDHFDRNSITNIKKNDTIIVAPASVCRQISGDTRPVRPGAQMNFDSVTIKAVPAYNI
                     GKKFHPRENQFVGYIIHADNIIYYHAGDTDLIPEMQDIRADVALLPIGGTYTMDAIEA
                     AQATKIIHPQVAVPMHWGTVSGSRADAEKFAQLANCQVVILNKGK"
     gene            24261..25538
                     /locus_tag="PLO58_03160"
     CDS             24261..25538
                     /locus_tag="PLO58_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73821.1"
                     /translation="MFKKGLILLILASAVFAQSNPPAIRPDSVGIYFNMSIQAYSEQQ
                     YDRYLEYTQKLIQVFPDDFTLQYNLASAYALNKDTVNAIKILNSLVDKGLGLVAEDDP
                     DFADLKNTPEFRALVKKIEKVKKPVKSSKKAFTVAEKDLIPRGITYDSQTKNLYISSL
                     YKSKIVQIDRNGKISDFVPERQDGLGPVTAIQADPQRRILWALSSFGIPNEKTPRELA
                     GTASVYKYNLNDGKLLKKYNLPRPNRHFLTDLTEDRAGNLYIADSGQRTIFRLDAQKD
                     TLEKFLELYKYPQLASLTITPDDKHLFVTYGGGILNIDVATGKITPLTSPDHILLVGC
                     DGLNFYNNSLIAIQTFLNRIVRFELSPDFSSVTDYKIIESNNPDFNIPSGGAIDGKYF
                     YYVANSQINSLDRSGNVMTPEQLKDILIFKAKL"
     gene            25560..26348
                     /locus_tag="PLO58_03165"
     CDS             25560..26348
                     /locus_tag="PLO58_03165"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HQO73822.1"
                     /translation="MKELVSLAESAARAAGAILRSYFQQEYTITHKGLDDPVTNADTE
                     ADAFLKEKLLSACPDYGWLSEESADSAERLTKSRVWIVDPLDGTKEFIAGRPEFMVSI
                     GLAENGQPVLGVLYNPINDELFSAYKRQGAFLNGSRIFSTKTKTLSEARLIVSRSELT
                     ENLWQPYRHFFKTLQPCGSVAYKLALVASGKADIHISLKPKNEWDICAAHCLLAEAGA
                     VLIGRDGMPIFYNLKYPIIPTGVIAGNKKLVVAGQKITQLKVCN"
     gene            26410..26685
                     /locus_tag="PLO58_03170"
     CDS             26410..26685
                     /locus_tag="PLO58_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73823.1"
                     /translation="MSSKTDKVDFSKNLSRHEKEMGIQISEMEKYKWICSKDQGRDVG
                     KMAYFEWTQKYGHKVRDWLESLSDEEIDRLFAELSDQVKNYIEEKSH"
     gene            26747..>28584
                     /locus_tag="PLO58_03175"
     CDS             26747..>28584
                     /locus_tag="PLO58_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HQO73824.1"
                     /translation="MESKDSTVTKLPENAYRPLKPGEEYIPVIPDAKIVPEVTPYSLG
                     WGLFYAVLFSMAAAYLGLKIGQVFEAAIPIAILAAGTSVLLKRKNALQENVIIQSIGS
                     SSGVIVAGAIFTIPGLYILGLDASYLQIFLASLLGGFLGILFLIPFRKYFVKDMHGEF
                     PFPEATATTEVLIAGEGGGAQAKVLVSAAIVGGLYDFCVAGFGFWKEVVSTSVFRWGA
                     LLADKLKWVLKINIGAAVTGLGYIVGLRYSAIIASGSFLAWWVIVPIVSYVAGLGAQT
                     PDAIFFDYVRPIGIGAIAAAGIIGILKSSKIIGGAFKLAGKELFGGSKGGTVEETSRT
                     QKDIPMKWVTVFLVLTLISIFFFFLFGVRITLKQAIVGLLIVAVISFLFTTVAARAIA
                     IVGTNPVSGMTLMTLLLSSFILVKVGLTGDHGMLAALLIGGVVCTALSMAGGFITDLK
                     IGYWLGTTPIKQESYKFLGSLFSAASVGLVILLLNKTFGFGGDSPLQAPQASAMAAVI
                     KPLMTGEQAPWVLYLAGVFLAIILEIIRVPSLAFALGIYLPIQLNTPLLFGGLVAYLV
                     SKSTKNEALAKARSDRGTLIASGFIAGGAIMGVVSAFIIFIGQNIFG"
BASE COUNT         8078 a         6235 c         6247 g         7824 t
ORIGIN      
        1 aaaaaggcaa cggctaaaga acatctcacg ggggttcgct ctgtcccagc gaatgtaatt
       61 taaggcgcaa aagttatcaa attcatataa catcagtgac ataggaaaag gtagtggata
      121 attacgaaag tgctattaca gcgctgaaaa ttccattttt tccgctggga cttcgctccc
      181 cagccggaga tgttcttaat gccgtaagta aattgaacgg tcagattttg ggttctcctt
      241 tttactaagc ctctttacta tttactcaac ctctttacta atgactaatg accaatgact
      301 aatgaccctt ctctgcctcg acctctttta cttattttag gttatttact tagaatttcg
      361 cttatccgtt ctacattgtc ctgagcggca attacgaata aaatatcacc ttttttcaat
      421 acagtatgac ctttaggcgg aatgatttga tcatctcgaa taatggcaat aattaaggaa
      481 tcgggcggta aagctaagtc gcggatttgt aaaccgtcaa ttttggaatt atcggataac
      541 tgaatttcaa agacatccac atcggttttt cgggtcgtga tcaattcaat cgaatggatt
      601 ggtttcggtc gagatggaat acagagtcgt aatttcgaag cgccccaggt aagtgtgacc
      661 ccttgtaata aacacgacaa aaaaacggca aagaaaacaa tattgaagat aaaatgattc
      721 tggtcaatgc cataaacagc tggataagtt gccaaaacaa tgggaacagc ccccttgatg
      781 ccgccccaca tcaggaacac tttttctcga aatttaaaat taaatggcag agtgcaaatc
      841 aaaatagtca gtggacgagc aactaagatc attatcgcag taattaataa tccttcttgc
      901 caaaccgaca ataagctttt gggaaaaacc agaactccca gcagtaaaaa gatcgcgata
      961 ttagagaaag tcgaaatccc ttcaataaaa ttgccgaccc ctttgcgata agcaaaatcc
     1021 gtatttccta accaatatcc ggcaaaaaaa accgcgataa tgccattagc tttgacaacc
     1081 tctgcacttc catacgccag caagcagacc gcaatgctta gcacgtgata atagccgcga
     1141 ttttccacct tgaggttatt gaatagaaac gcgccgactt tactaataag ccagcctacc
     1201 actattccac caccaaattg ccagattaaa ttgaggataa aaagtccgat gttctgggga
     1261 tgccctgtaa ttatttgaat aaaaacaatt gtcaacatta tagccatcgg gtcattggta
     1321 gccgattcga cttccagtgt caaggaaacc cgctttttaa tggcacgctg acgcataatc
     1381 ataaatactg ccgcggcatc ggttgaagaa atgatggagc caatcaacaa cgaataaagt
     1441 aaatcgagcc ccataataaa atgtatcaga agtcctaaaa ttcccgccgt cagagcaata
     1501 ccgaacgtcg cgagtgtcaa agccggaccg aaaacagatt gcactatttc gcgacgggta
     1561 tgaaaaccac cttcgaataa aatgaatatt aaaaagatat tggctacttt ttgagccagt
     1621 acagcattgt caaaataaat cagattgagc acatcactgc cgcatagcat cccgatcccc
     1681 agaaatagca gaagcaccgg aaccccaaat tttgaggtta tctttgtcga gtaaatggcg
     1741 gcgattagca atatggcaat cagtaagtac atgaattttc cttaatcaaa gagtaaactt
     1801 ggaagagtta accttaactc cgccgatctg gtatgcaatt aaatttatgg catacgaata
     1861 tttaaacatc ttatcattaa ttgatgatgt aaagcacttc actatataag aaaaatacaa
     1921 ttaaaatcaa aatgaatatt atcaattcaa gttttatgtg ccattctatg aacggtttaa
     1981 gcacccggca aggtggaaat tggtcatggg tagcgggggt gctgaaaata atatagtact
     2041 tgatttttag tttttttatt ttgccataaa gctcttctaa tttaaaagtg atttattcac
     2101 ctacttacct acaagattta gtttagtaat aaacttaatt ttgactaaat tatattctgt
     2161 gaaattattt gaaaatgtca gcttgaatag tatttatttt aagccggttt acaaacttac
     2221 caatgatagc ctggatatct aaaccggcag acacttgagg ttaacttgaa agaaatcctt
     2281 caggtaataa tagacgaaga aaaatctgcc cgcgaacgaa tagaaaacgc tcgtcttcaa
     2341 gcccgccgcg atacagaagc cgctcagatt gaagcccaaa aaaccattga aaccgctcgc
     2401 caattggcat tacaggaagc cgaagcgctt attacgaccg ccaaatccga agctgaatgc
     2461 caacacgccg agctgttaaa tgccgctcga gcgcaacaaa acgacatttt caataaatac
     2521 catgctgaaa tcgccgccgc tgtggatttc ctttttcaaa cccttccaat tggcaaaaag
     2581 gtcgcctaat gagtgctgtt agcacttacg gactggtaaa tgctcgcgtc cggacacgac
     2641 gctccgcctt gctttctgct tcatcttata aggctttagc cgccgcaact gatttaccgg
     2701 aaatgcttaa tctcttaacc cgctataaaa atcgtctcag tttcgaaaac cttcctgatt
     2761 attcgattga agctttggag cggttattaa tccaagagaa agcccgtcag atacttgatt
     2821 tgaagtactc cagtcgcgga ccagctcggg acattatggc tttgatgctc tcaagaattg
     2881 atgctgaaaa cgtcaaaact attctcaggg cttggcaaaa atccgaccag aatgtcagta
     2941 acattatcga atgcaccggc ttggcagttc tgccgattac aaaaatgctg agtgcggagg
     3001 atttaaccca atttgccgct tatatcaatc agctaccgtt ttatgaagct ttcctgaaag
     3061 ccgctaaagg tttcgaccga agcaagagcc tctttgaaat tgaagttgcc attgataaaa
     3121 gcctgtatca gtccctttgg gacctgactg ctttattgaa taaaaccgat caacatatag
     3181 tacgacgaat ggtcggcatc gaaattgacc tgaaaaacct ggattggatc gcccgcatcc
     3241 gtaaatatta ccagtctcaa attaaagtca ccgagacaag tctaataccc aacggctatc
     3301 gtttgcctta tgctactctc cgaaaaatga ttactaccgg ctcaatcgaa gacggactgg
     3361 aaaaaatatt accgggattg acgcccatta ctgcgcagga acgtgatcaa cttactgctc
     3421 ttgaacgctt agagcaattt ctcaatcagg cgctttttca tgaagctaac cgcctttttc
     3481 tggaaaatcc actttctatc gcctccacaa ttggctacta ctatctagtt catatcgaaa
     3541 cccggaatct ctgcacatta ataaatgcta aatattataa attagcaccg caggcggttt
     3601 taaataaatt ggtatattga ggataaagat gtttttccca gaaaaaatgc ttttggtcaa
     3661 tgcgctcttt cccgacaaat atacgcccaa ggttgtccgc atgattatac atcagggcga
     3721 tttgcaaatt gtggatgccg cccaaactaa tccttggatc aaagacctcg cacgaacata
     3781 ttcaacggaa gaatcctctc aatctcaagc ccgtcgggaa aaagtggaag ggttagttaa
     3841 aacccttaaa ttaaccgacc aactccataa ctttcaaccg ctaagcggtg attggatgga
     3901 aattgatcag agcctcgaga ctgccattgc tgaaatcgaa tcagttagtc agaggcgcga
     3961 cagcctgcaa aaggaatttc agcgtctcaa tgaaatcaaa acccgctacg atcgctttcc
     4021 cggtctcggc aaagcccttc aaatgccggg tgactattcc tatcttgcta ttgaaacagg
     4081 cgaaatcccc gacaataacc taccgctact ggaagaacaa ttatccgcta ctctacatgt
     4141 aattctaccg atcgggagac agaaaggaat gacctcactg gtggcaatcg ttctcaaacg
     4201 cgatgccgaa gttcttcagg cggcgctcaa agccagcggt ttccagccga ccaaaattga
     4261 aaaagaattg cccagtctcg ccaccgaatc aattgaaaaa ctcgaaaaag aactgaacga
     4321 tctcaatgaa caactgcgtg acgccgagga aaaattccag gcggtagtca atcgccatcg
     4381 cgcgctccta atcggcgttt acctacgtat tcgtcaggaa caagttcaag gccggatgtt
     4441 acatttcttc cgccgtactg aaaagacctg tcttttttcg ggctggttgc cggatagccg
     4501 caaggaggtt tttgtcaaag aattgcagaa agccacccaa aatcgggcta tcgttgacct
     4561 gataccggct gaatcgcatg aagcagtcaa agatggtagt gtggaagtcc cggtcaaatt
     4621 aaataacccc gcctttttca aaccctttga gatgctgact tcggtctatg gtctgccgaa
     4681 gtatcagacc attgacccaa caccggtgtt tggtctaagt tttcttttga tgttcggaat
     4741 gatgtttgga gatattgggc acggtctggt cttggcattg cttggcttag cgttgctatt
     4801 aaaaagtcac aaagatacat tcaaatcggc cggctattta ctactctacg ccggtggttc
     4861 cagtattatt ttcggttttc tattcggtag ttttttcggg atcgaagatt tatttccaac
     4921 catctggatg aaaccgatga atgacatcaa tcaattgttc aaagttgtga tctacgttgg
     4981 tgccgggatg ataaccgtct cgattattat aaatttaata aatggcatcc gtagcggcag
     5041 aattctcgat tatatttttg acaaagccgg tttactgagc ggcattctct attggtgcgg
     5101 tatcatattg gtaagtcgtc tggtactcac gaaaactaat ggtggagttg gcctttttgt
     5161 gatctatctc tttgtggctt gcgctctgct tcttttcttc cgggaaccaa tccttaattt
     5221 aatccgcaaa aaccggcgtc tatatagttc gggagttgtc accggattaa tgcattcgct
     5281 tgtagaaatg cttgagattg tgctgggatt tttggctaat acggtttctt tcatccgtgt
     5341 gggagcgttt gggttagccc atgccggatt atttatcgcc atattcgctc tttcggatat
     5401 ggtgagtggc aaattaagtg gagtagcttc tatcttaatt ctaattttag gcaatatttt
     5461 catcattgct ctggaaggac tagtagtaac catccaggca attcgtctgg aattttatga
     5521 atttttcaat cgctttttcc gtccggcgag ggttaagtat caaccaatca gagagaatcc
     5581 caacctggag taaatattct ctaataatat gaaaaataaa tttgcggagg taatgcaaat
     5641 atgttaaaaa tcaaatcctt taaaaaactg ctggcaattg tgattacgct gggtttcgtg
     5701 gcaattggcg gaacttcact actggtgctg aaggctattg cacagactcc ggaagaaacc
     5761 gcgacgagtg tagtagagag cactgaaacc acagtcgagg ttgctccgaa tacggctcgc
     5821 gattccgata cgatcaaatt tggttttatt gccgccgctc tggcagttgg cattggttcg
     5881 atcggagccg ccattgccgt tggcaatatt ggtaccgcgg ctatgggcac aattggcgaa
     5941 aaaccagaat tgtccggtag cgctctgatt ttcgtcggtc tggcggaagg tatcgctatt
     6001 tacggattga tcgtagccat tatgattctc ggaaaattgt aatttgccgg agatgttgtg
     6061 aaatatttcg tgattggtga tgaagagact gttctgggat tcagcctagc aggtgcggat
     6121 ggaattgtgg ttcacgatgc ggcttccgct cgacgcgctc tggctggtac tatggagcgc
     6181 gaagatatcg gcatcattat aattaccgag cgcgttgcta caatgattcg ggaagaggtc
     6241 aatgcttatt tttatcatag cccttatccc ttgattgttg aaatccctga tcgccttggc
     6301 ccactggaag accgcatttc agtgcgagat gttgtccaaa ctgcggttgg tgttcggtta
     6361 gaatgaggaa ttatgcctga taaaaacgcc actctggaag ctattgtagc tgaaattcgt
     6421 cagatgtcgg atgctgaaat tgaagccatc cgtcagtctg ccagccgcga gcaagcccaa
     6481 attgctcaac gtgcccgcca agaggctgaa aaaattcgcg ccggaattat cagtaaagcc
     6541 cagcaaaatg ccgagaaaat tcgccagcga tcagcggctc aaaccaatct tgaaatcaaa
     6601 aaattgaatt tgcaatcacg acttttaatc acggccgaaa tccggcgtca atttgaagaa
     6661 cgtttgtttg ctttgcgcca atcgcctgaa tatgcccaat tgctgaaaga gctaattgtg
     6721 gaaggaattg ttggcttggg agttgatcac attctcatta gcgctggcga tcgagagcac
     6781 accatcctaa ccgatgaatt tcttagatca attgaggcat cttttaatcc accggtgcac
     6841 cttgaactgg agcctgtagt tctcaatgag ccgggggtca ttctttattc ggcagataag
     6901 cggaggcgat tcgataaccg cttatcccgt tatgttcagc gtgtcctgga aaaatatcac
     6961 tgggaaatta tgcagaaatt ctcggaaaat aattaaaaga ggtaaacggt ggcaatagtt
     7021 attggaaaag tcacccgcgt tttgggacca gttgttcatg caatcgcaac tgctaaggtt
     7081 cagatgctcg aatttgtcga ggttgggacc gaacgcctga tcggcgaagt tgtgcggctt
     7141 cacggcaatc aaatcatcat tcaagtttat gaagatacta ccggtttaac gcccggagta
     7201 gaagtgtacg gttccggaat gcccctttca gtggaactcg ggcccggtct gattggcact
     7261 atatatgacg gtgttcaacg cccgctgacg gaaatcaaga aggtttccga tcaatatatt
     7321 catcgtggca tcaaagctcc tgctctcgac cgcaataaaa aatggtcatt cgaaccagct
     7381 gtaagtcccg gtacaatcgt tagcagtggg cagataatcg ggtcagtaaa ggaaacggtt
     7441 tccattaccc aaaaaacatt agttccgcca aacatttccg gcaaggtcat tagcattgcg
     7501 ccgccgggag aatatcgtat cgaaaatgaa atttgtgtta tcgaaaatgg tcaaatccga
     7561 catacattgt ccctttttca gcgctggcca gtgcgtcagc cacgaccaat taaaaaccgt
     7621 ttgtccacca gtcaaccgat tataaccgga cagcgcgtta ttgataccct tttcccgatt
     7681 gccaaaggtg gcacagtcgc tattccgggt ggatttggta ccggtaaaac tatggttgaa
     7741 caccagatcg ccaagtggtc agatgccgat gtcgtcgtct atattggctg tggtgaacgc
     7801 ggcaacgaaa tgactgatgt cttgactgaa tttcctcaat taattgatcc gcgcaccggg
     7861 cgaccgctga tggaacgcac cgtactgatt gccaatactt ccaatatgcc ggttgccgca
     7921 cgtgaggctt cgatttacac cggcatcacc attgccgaat attttcgtga tatgggttat
     7981 cacgtcgccg ttatggctga ctcaacttcg cgctgggcgg aagctttgcg cgaattgtct
     8041 ggccgtatgg aagaaatgcc agccgaagaa ggtttcccag cttatttgcc aactcgctta
     8101 gctgaatttt acgagcgcgc cggttatttc gaaactctca accaggaaac cggctcggtt
     8161 accatcgtgg gcgctatttc accgcccggt ggtgatttct cggagccggt gactcaacat
     8221 accaagcgct tcgtccgcaa tttcctggcg cttgatcgcg atctggctaa tgcgcgccat
     8281 tatccggcga tttcctggct cgattcttat tccgattatg tcgaaatggt gactgcctgg
     8341 tggcaggaga aagccgatcc ggattgggct gagttgcgac aggaaatgct cgatcttctc
     8401 cagaaagaag ctaaacttca acaggtcgtc aaattagtcg gtgcggatgt tctgccggaa
     8461 tctcagcgcc tcatcctaga agtctgtacc cttttcaaaa acgtttttct ccaacaatcg
     8521 gcttacgacc agatagatac ctattccact gttcaaaaac aggcgaaaat gctccgcatc
     8581 atcgttacct tttatcggtt atcggccgat gtcctcagac gtggagctac tctttataag
     8641 gttaaacgca ctaaaacctt tcgtgccatt atgaatatga aatatgaaat ttccaatgat
     8701 gagattgata aattagatca aatcttggca aatctaaccc aagaattggc tagcctcagt
     8761 cgtccaaatt tttaaggctt tagcaaaatg ttgcaaatca aaaaaattaa tgaattgaat
     8821 aaatataacc aggctgaact cttgaaatac cgggaatata ttggaattca agaagttgct
     8881 gggccactaa tttgtatcga aaatatccat aatgttggct ataatgaatt agtcgaaatt
     8941 cgggacgaac gtggtgaacc acgcattggc caggtgctgg aagccaacca atccgccgcc
     9001 gttgttcagg ttttcgaagg caccaccggt ttatcattag ccggaacagc agtgcggttt
     9061 cacggcacgc ctttgcgcat tcccgttacc aaagaaatgt tcgggcgaat cttcgatggt
     9121 ttgggacgcc caatcgacgg tggaccggag cccttgacca atgaattctg ggacgccaac
     9181 ggtgaatcta taaatccaac cgcccggaca tatccaacta cttttattca gacggggatt
     9241 tcagctattg acgggatgaa cactctggtc aatggacaga aactgccgct attttctggc
     9301 ccaggtttat cacacgatcg tttgacggct caaattgttc gtcaagctaa aattgtaggc
     9361 cgcgaggtat cctttgccat tgtctttgcc gcgatgggca tcaagcacga cattgctcaa
     9421 tacttcatag ataatttccg ggatgccggc gttctggaga atgtcgtaat gttcctgtca
     9481 ttggctgatg ctccgtcggt tgaacgcctg atcacgccgc gttcggcttt gactatggct
     9541 gaatatttag cctttcatga aaatatgcaa gtgctggtgg ttatgaccga tatgaccaac
     9601 tattgtgagg cattacgaga aatttcgatt gcccgcggtg aaatcccgtc ccgcaaaagt
     9661 tatccaggct atctatactc cgacctagct tcgctttacg aacgcgccgg tatggtagat
     9721 aactcaaccg gttcgattac tcaagttccc attctttcga tgccgaatga tgatatttcg
     9781 catcctattc ccgatcttac aggatatatt accgaaggac aaatcgttct ggatcgcgaa
     9841 cttttccagc agggaattta tccgccgatc gcccttttac cttctctttc gcgtctgatg
     9901 aaagatggaa tcggggaagg tttgacccgc gccgatcatg cgcatttagc cagccagcta
     9961 tttgcggctt atgcccgggt gaaggaggtg cgtgctctgg cttccgtcat tggtgaggaa
    10021 gagctcagtg cagttgacca cagttactta aaattcggtc tggctatgga aaacaaattt
    10081 attgctcaga aagaaaccga agatcgttcg atttatgaaa ccctcgattt aggttgggag
    10141 atgttgtcgc tcctgccgcg ggaagaatta caacgcatta ctgaagaaga agcccagcag
    10201 tatcaccatt accaaaatga aaataaataa ataattagta aaatggcaac ccgcatcaaa
    10261 ctcgtcccga ccaaaagtaa tctgctcaaa ttaaagcgcg acttgagctt tgcccaggaa
    10321 ggctatgaac ttttagagca gaagcgccag gtgctggttt cacaactgat ggcaatggtt
    10381 aatgaagcga ttgtggcgca cgaaaaaatg gatacggctt tgcgcgaagc gtatagtgct
    10441 cttcaaaatg ccattatttc cgatggaaaa agcacggtca agtctatggc gcgagcagtt
    10501 cggatcaaat cggatatcca gatttacaat ctacgggtaa tgggtgtgac aatcccgaaa
    10561 gtcgaggttg aaatagagga tgatgcgcct tactatagtg ccggtaattc gtcattctgg
    10621 gttgacgaaa gtattttccg atttaaacaa attctcgagg tgatgggacg ctttgctgag
    10681 ttgcgtgttt cgcttttacg cctggctcag gaagtccgca agacccagcg gcgagtcaat
    10741 gccctggaaa aaattgctat ccccgattac caagaaacta ttaaatttat ttcagattct
    10801 ttggaagaaa gcgagcgtgg aacacttact tcgatgaaat acatcaaaga acaccttgaa
    10861 aaacgaaagg atgcctaata cgcaatcggg acgtctcatt tcaaaaattc tcctgctgac
    10921 tgatggttca gagggatgta tttcggttgc caagtatggc atcatccttt caaaaattac
    10981 tggagcggct ctcaaagcaa tttatgtcgt caacactgat ttactcaagc agctcgagcg
    11041 cgctcgtatc tttgtaaaaa tcgaagaagt cgattacgaa agtgatctag aagatgacgg
    11101 tcaagattat ctgcaagaca tcacagaatt ggccgcttcc aagggcgttc atcttgaaac
    11161 cgaattattg aaaggcatcg tccataaaat cgcaatccag aagatcgaag aatggaacgc
    11221 tgacctcctg ataatgggtg aacttgagcc aggtctgccc cgtcaggaca tctctcacga
    11281 cgaactcggc ttgcttttac gtaaagcgcg ctgttcggtg ctcgtagcta aagagcagag
    11341 gggtcttgac gaattatttg aaactttaac ctgaattttg tcaaagtaaa acgaggagct
    11401 ttttttaaaa tgaaactttc caatctgatt aagcgcgagg cgattgtcgt accaattcag
    11461 aataccgaaa aacaattagt aatcgaggaa ctactagaag ccgccgcggc cacaggtcag
    11521 attcttgacc gcgcaaaagc tttacaagcc cttttggatc gtgaaagctt aggcagtacc
    11581 ggcctcgaaa aaggactggc gatcccgcat gcccgcacta atgcggttga cggtctagta
    11641 atatcactgg gcgttgcccc ggatggcata gattttcaag ccgctgacgg cggtctttct
    11701 taccttttct ttctattgct ggcttcggat gcattgacct caacctatgt tcaggctctt
    11761 gcattgatcg cacgccttta tcaacaggat gactttcgtc gtgaaatatt gaatgctacc
    11821 actcctcaag agattatcgc cattattcgt aaagccgaag aataacgtgt ggcttcgcac
    11881 cttaatttga gcgacaatat catcactatt aaaaaccgat aacagataac attaaaaagg
    11941 agaaacaatg aacacaatag aaaaagcctt gatgcatgga ctattcgttc atctacatca
    12001 caatattgat aaagtaattg attttaaaag ctttcaggaa ttaaaccgcc aggtcgctaa
    12061 agtttggcca ggagcattat tgatcggccc ggaagctaac gacgatgccg cagtttttca
    12121 aatcccgggt atgccgggtt acgtagttgg taaaatggaa agccactgtt cgccctgtgt
    12181 gccacggccg tatgatgccg ccgccaccgg tgccggtggt gcgatgcgtg atgtggtcgc
    12241 aatgggcgct cgtcctattt tcctgatgga ttttatcggc acgcgaccac tggagcagga
    12301 agtcatagtt gggccatgcg gtttttcggg gcgttgcacc tgtggcaaat gtaaggttat
    12361 gaccagtcag gaaagagtga atattatggt caaaggtttg ggggatatgt gccgaacgat
    12421 ggatgttttt atcattggcg gaggtttctc tacttctttc tccgatatcg taccggctgt
    12481 cgttgtttcg gttatcggca aactggttac cgaaaaacca cttaccaaac cagccaaaaa
    12541 tgtcggtgat aaagttatca tcattggcgt gaccggcaat gacggcaacg acaccctttt
    12601 ccgggcggga ttggtagaag aaatgcgccc ggccattgcc ctatttaaag aagaaaaaac
    12661 cgcgatggaa gccgccttgg cggcttttgc gactggcaag attaatgcct gttccgatct
    12721 cggtgccgct ggtattggtg cggcaatttg cgaatcagcc cgtttcggtc ggcgcggtgc
    12781 caaagttgat ctggctaaag tcccagtttc tgccaaagat attactccgc aggaaatatt
    12841 aatttgcgag acccaggcac gctatgtcgt tcaggttgcg ccggaatcag tagatgaagt
    12901 gctaaaagcc attcgcacca aaaccgagca tgtcgccgta atcggagaaa tcaccgatac
    12961 ccagcaggaa gttttcgaat acaaaggtga gattattgcc acgattccca atcaaccctc
    13021 acccgaaact ttagcaattt tgaaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnact agctctaata caatggaaaa tattaataaa aataaacagg tagtaaaaat
    13201 tgtaaatgaa cctgatttag aataccaaat aaaaatatat tctcgaaatg caaagattgt
    13261 ggatgatagt tgggatttta gaaattcaaa taccaaagat tttacgcatt gttttcactc
    13321 atatccagca atgatgattc cccaagttgc tagaaggatt atagataatt tgggtaagaa
    13381 atcaaaaatc ttattcgatc cttattgtgg gacagggacg tctttagttg aagctaatct
    13441 aaaaggtata gatgctattg gaactgattt gaatccttta gccaggttaa ttgccacggc
    13501 taaaactaca aagatcgata tcaaaatact tgacttattt cttcaggatt ttaaggatta
    13561 cattttttct atagactttg atattggcaa aatatattcg gaaattattc cagatataaa
    13621 caatatagat tattggttct caaaagttac tcagcaaaaa ctatcattga tactaaaata
    13681 cattgataat attagcgaat tttcaattaa aaactttttt aaagttgctt tctctgaaac
    13741 agttcgcgag tcttcatacg taaaaccggg agagttcaaa ttagtcagga gcaaaaatta
    13801 tttagataaa tctgaacctg acgtttttag aatgatgctt tcaaaattat taagaaatag
    13861 aattggactt atcgaattta tagagtgttg taaaaataac gctaagacgt cggtatatga
    13921 tttcaataca ataaatgaaa ttccaaatgc tattcttcca acagagattg tagatattgt
    13981 tgtgacatct cctccctacg gcgattcacg cactaccgtt gcctatgggc aatactctag
    14041 gttagcaaat gaatggcttg gttttaaaga tgcaaataaa atagatagaa ttttaatggg
    14101 tggggagaag aagaagcata ttcccgtatt taaaagtgga atattaaacg atgtaataga
    14161 tacaattcag aaagaagata aagaaagagc tcttgatgta atttcctttt atgaagatta
    14221 tgagaaatca attaaaaatg tttcagctac cataaaaaag ggtggttttg cttgttatgt
    14281 tgtgggcaac cgaacagtta aaggtcttac tattcctaac gatgaaataa cagctcagtt
    14341 atttgaagaa aataatttca gtcatattga aactattgtt aggaatatac ctaataaaag
    14401 gatgccttta aaaaatagcc cttctaatgt cgttggcagg accgcttcaa caatgaaaaa
    14461 tgaatatata gttattatgg caaaaaattg atatgcagtt aaaagaacta ataaaaaaat
    14521 tagaagccct tgagcagaaa ggatatcttg aaacttcaag acaaggacca actggtattg
    14581 ggcatctact agaaaaagaa atgggattat ttgaaaacaa tattcctatt ccagatattg
    14641 ggggtagagt tgaactaaaa gctactagac ggaatgtaaa ctcgctaata acacttttta
    14701 cttttaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa taagggggtt
    14821 tggcttgtca aacaaaacaa tattatcgaa aaatatggat acaaagatga tagaggtaga
    14881 caagcactat ataacattgt taataaaaaa acgccgaatt cccagggatt ttacctcatt
    14941 tcagatcctg aaaggcactt gatcatctta aaaaacatct atgaagatgt gaatattgcg
    15001 gagtggagca cctatgtaat tgctggtaaa ttcatgacca agcttgatag attactttta
    15061 ataattgccg acaataaaat agaaaatgga aaagaatact tccactttaa tgaggcttat
    15121 ctgctggaaa atccaactcc agaaagattt ttattagctt ttgaaaaaaa tgaactgatg
    15181 attgatttac gaatgcactt gaagccatcc ggtggcgtca gaaatcatgg gactggattc
    15241 cggatttcag agaaaaacct tattgatttg tatgaaaaga aaaaaagatt gatctagatt
    15301 ttatttttct actcagctgt cgttagaaaa ttgtatttaa aaagaggatt tatccttaat
    15361 caattgtaag ccgagttctt tcaactgttg ttcttctacc ggagctgggc taccatccat
    15421 cagggaagcg ccgctggtag ttttcggaaa agcaatcact tcccgcaaat tttcggcact
    15481 cgccaatacc ataaccaggc gatcaaaacc tagagcaatt ccgccgtgcg gcggcgcgcc
    15541 gtagctcagt gcctccagta aaaaaccgaa tttttcctgt gctgtttccg gattgatctg
    15601 caagacatcg aacatccgct tctgcatttc aacagtatga ttgcggatac taccgccgcc
    15661 gatttcgtgg ccattgatca caatgtcgta tgcccgtgcg tgtacttgcg ccggattagt
    15721 cgctaataat tcggcttctt ccggcacagc ggcagtgaag ggatgatgca tggcgaccag
    15781 acgattctct tctgcgctcc attccagtaa ggggaagcgt gtcacccaaa ccggcttgaa
    15841 aacattcgcc ggaatcaatt gcaagcgttt ggcaatttcc agccgcagga cgccgagcgc
    15901 cgaataggtt ttttcccgag aatcggcact gaaaaaaatt atatcaccgt cattagcatc
    15961 gaactcagct ataatttcgc gggcgaccgg cgtcaagaaa cgagcaattc caccttctaa
    16021 accgtttgcc gttactctgg taaaagccag tccttgaact cccattttct taacctgctc
    16081 ggttaattca tcaattagtt tgcgagtaat cgaagcccct tgagggaaac aaattccggc
    16141 aacggttcct cccgaagcca atgctttgtt aaaaacttcg aattcaccgc gctcgacaat
    16201 tttatccaaa gtatgaattt cataaccgaa acgcaggtcc ggtttatcgc tcccgtattg
    16261 ccccatagcc tcatcatagg gaatgcgggg gaaaggcgat tcaagattaa ttcctgccac
    16321 ttcgcggaaa acgcggatta gcatttcttc ggttgactgt aatacgtcct cttcctccac
    16381 aaatgacatc tcgacgtcaa tctgagtaaa ttccggttgg cggtcggcgc gcaaatcctc
    16441 atcccggaaa cagcggacaa tctgaaaata gcgatcgaag ccagacacca ttagaatctg
    16501 cttatattgc tgaggcgact gcggtagagc atagaaccga ccttgacata ggcgactggg
    16561 cacgaggaaa tcacgcgctc cttcgggagt actacgagtc aaaaaaggag tttctatctc
    16621 atagaaatta tgttcataat aaaaatttcg cacgctttga taagtcttat gccggatggc
    16681 aatattgcgc tggagctcgc tcgtccgtaa atcgagatag cgatatttca agcgcaaatc
    16741 ttccagccct gttttgcgat cggttaccag gaaaggctga gggcgtgctt catttaaaat
    16801 ttccagctcg atagccagta gttcgacggc acccgtagcc atttcgcggt tgatggcatt
    16861 ttccggacgg cgtctaatct tgcctcgcac ggcaattaca tcttccaaac cgagtttacg
    16921 agccaaagtc attatttctt cacccgattg gggatcaaat accacttgcg ttagaccata
    16981 acggtcgcgt aaatcgacaa agattaatcc gccatgatcg cgccgacggt tgacccagcc
    17041 attgagaatc acgatttgtt caacgtgttc gacgcggggg tcgccgcagg tcagagtacg
    17101 ttttaacatt gcttatccat tatttaaata atctttcaaa cgggcaaaat taatgattcc
    17161 atatagtttt aaccagcaga aaaatgattt cgactaaacc ggtagctctt aatagtaatc
    17221 accgaaaaaa tatccagaac gtaatcatca gaaaaagctg gttgatgatc atcggataat
    17281 cccagtcgtc ccacctgata ggcattaaat tggcattgat cagaccggaa aatttcatag
    17341 agtaatttgt ggttcaataa ttaacgtttt taatagggaa aaatatacag ttgtcccaaa
    17401 atagcaaatt ggggtcaatt aatgcggtaa tttttattgc ggcagattcg cgtcatccgc
    17461 tgccggcttt gccgcatttg gcgattgggc ttccagggga taagggaaat gcatttcaca
    17521 gtgtccttcg aaaaccgcgc cgtcttcgat cacgatctga gcggctttga tatcgccgcg
    17581 taaaaaagag cggtcgccga gggttacttt tccggtagct atgacatccc cttccacatg
    17641 cccgccgata ttaaggttag cgctttgaag gttgcctttg atttcagctc cggggaaaat
    17701 tgtgatggtg ccatcggttt cgatatcacc gctaattttc ccgtagatga ttatatcgcc
    17761 ttccagccgt aagctcccgg taatgatggt tttctccccg ataatagtat tgatggaaga
    17821 ttcttttttc ttgattatgg cagacatatt aatattctcc ttcatactta agggaaatat
    17881 cggctttttt ataggaataa atcaattcct ggggattgat cggaacacct tcgcgccaga
    17941 tttcaaaatg caggtgtggt ccctgacttt ttccggtgtt gccaagatag ccgattacct
    18001 cgccgcgttt cacatattga tgagcgacta cactattacg ctgattatgg ccatagatgc
    18061 taaaatatcc atcggaatga tagaggatga cagtatagcc gagatcataa gtccagttag
    18121 aaaaaaccac taccccggcg gcggcggctt taatgggagt tccttcggcg gcggcaacat
    18181 ctacaccata gtgattctcg ctggtcatta ctttatcatc aatgaacccg cgcgtaatat
    18241 aaccatccac tggcgggtaa atcggaatgt tttctaaaaa actgatctga actaaccggt
    18301 tttgatcggg atcattcttt aggtaattga cggcttcacc ggagacagaa tcgctgggag
    18361 tcaggacgag atcggtgccc aataccgaac gaatatattg gtccatctgg cgaatctcat
    18421 tgtaatcact cagtatttga gatactttga gacggtcctg aaccagttgg gcgttttcaa
    18481 gagtaacctg ccggtatttg attgctttgg gtaaggtaat cagaatagtg actatcaata
    18541 gagctagaaa agctagcgaa ccgataataa tcaaccgggc agtcgtgtga ctaaaagtcc
    18601 agataatagg ttttgcgcgg ctctccgaaa gtaagtgtaa ggtatatttt ctcttcataa
    18661 tgttttttcc tcgatgattt tgaatatttc cagcagacgt gccaggtctt cgcgagaata
    18721 aaattcaatc gtgatctggc ctttgttttc gtctccctta atttttacct tggtaccgag
    18781 aatgtgaatc aatctatcct caatggcttt ggtagtaggg gttttgactg gtagcggtgg
    18841 cttttctttg agaggaatat tttgaaattt cttagatagt ttttcgactt cccgaacgct
    18901 caaaccttct cgctttattt tttgccagag attaatttgt tgttgtggat ttataatgga
    18961 gagaagcggt cgggcatggc cggcgctgag ttcgccagac cttaaatcag ccagaatggc
    19021 atccggtagg ttgagcaggc gtaaggaatt agtgatcgtc ggtcgttctt tgccaacttt
    19081 tcgagcgatt tcttcgtggg atagattgaa tgtgctggct aaaatttggt aagcgcgagc
    19141 ttcatcaacc ggattaaggt cttcacgctg gacattttca attaaagcca attccagcat
    19201 ttcagcctca gtggctaccg gcataatata agccggtatg gtcgtcttac cgagttcttg
    19261 acaagctcgc agacgacgct caccggcgat taactcaaag ccgccatcga cctggcgcac
    19321 ggtgatcggc tgaatcactc ctttttcccg aattgacata atcagttcag ccatcgcctg
    19381 agccccaaat tcctggcgcg gctggtgtgg attgaacttg atttgagcca cttcgatttc
    19441 agccatcgta taaacctgtt ctattccttc cggaaattca gggattagtg cgctaatacc
    19501 tttacctagc cgtttgcttg tcgtcattga gtagttctcc taccagattc atatagtcct
    19561 gagccccagc cgaggtagca tcataaagga tgatcggttt accaaagctg ggtgattcac
    19621 cgaggcggac attgcgccgg accacagttt tgtaaacctt tcctttgaaa tgactgcgca
    19681 cctgttccga tacttcatgg gaaagacgca ggcgactgtc atacatcgta atcagcacgc
    19741 cctcgatttg taagttgcgg tttaaactac gctggaccag gcggatcgta tttagtaatt
    19801 gcccgagccc ctccatcgca taatattcgc actgaattgg aattagcacc gaatctgaag
    19861 cggctagact attgatcgtc aagagtccca atgaaggtgg gcaatcaatt attacatact
    19921 tataatcacg ggttagcaat tcgagagatt tctttaaaac taattctcgg ccgattagtg
    19981 aaaccatctc aatttcagcg cctactaaac gggtggaagc tggcaaaata tcaagataag
    20041 gaatttcgga aggcataatc gccgtattta tatcaaagcc ttcaattaat acctcataaa
    20101 ccgtattttc caaattgcga taatctatgc cgcatccgat cgtggcgttc gattgcggat
    20161 ccatatcaat cagcaaagta ggaatttcgg aaaccgccag tccggcggca acgttaaccg
    20221 ccgttgtggt ttttccgaca ccaccttttt gattagcaac cgtgattatt ttggccatct
    20281 agcttctatt aatttcttta atttcaatat atttcaagta ttaaatatta ttaaaaaacc
    20341 aagcaaattt aaaccctgcc tactctaaaa aacaagttaa aagccttata gcagagagta
    20401 agtcttccca caacactgag ttaatgagcc cgttgccaga aagtaggaag gaatacaatt
    20461 aaaattgaat aaaattccaa tcgtccaaat aacatactag ccattaatac tattttattc
    20521 agaacggaca gagccgcata actctctatc ggaccaaata aaccaatgcc tggtccaacg
    20581 cctcccaggg ttgtagccac ggcaatgagc gctgattccg gggcaacgcc gcctaaagcc
    20641 acgatcatac tcgcgaccaa ccaaaaacac agatacattc caatataaac aatcgcattc
    20701 tggattgttt tttcatcaat tgggcgttta ttaaaatgaa tagtcgtgac tacccgtgga
    20761 tggattatct gaatgagctc acgatggatg cttttgataa gcagaatgat tcgcgaattt
    20821 tttatggctc cgcacgtcga tcccgggcaa gccccgatca gcattaaaat taacaaaaca
    20881 atatgtgtca ccggttgcca gatttcccaa tcagttgtaa cgaatccagt cgtcgtatta
    20941 attgaaactg cctgaaatac tccatagcga attgcttctg accaagaatt gtaagtatgc
    21001 gaacgcaaat taatcactat gaccaaagat gcgaccgcca taatcgcaaa gtagatatag
    21061 actgtttctg agtaatcccg ccagttcttt cgtgattgga acattcgtag gtagagagtg
    21121 aaatttatgc cggagagtat cataaaaata acgacgacta tttcaacata tacattgtta
    21181 taggcggcga tgctggcgtt tttgggtgaa aacccgcccg tggcgatagt cgccatagcc
    21241 gttaatgccg catcgaaaaa gggcagacct aaaattaata aaagaacggt ttcggagact
    21301 gtcaggataa aatacaccgt ccataacaaa cgcgcggtat ctttgatttt aggcgtgatt
    21361 tgttcattgg caagcgaaga ggattcgaag ctatacaaat tgcgtccgct aatattaata
    21421 gctggaagca ctactaacgc caggattatg attcccatac caccgatcca gtgcgtcaga
    21481 gtgcgccaga caagcagact aatcggtagg ctttcaatat catttagaat cgaagcgccg
    21541 gtggtggtga atcccgaaac cgactcaaac agagcgttta taaaaccatc gaaatatccg
    21601 ctgaaataaa acggcaatgc gccgatcaca gatgccccga tccatccgat aaccacgatt
    21661 accattgcct cccagcgctg aatattaaaa ttgcggggct tgcgaatctg cattaataat
    21721 ccgacgcccg tagcgactgc aatactgaaa agaaatgccg gagtggccgc gtcatgttgg
    21781 aataggctaa tcagcaacgg caaaatcagg aacaatccta atatggtaaa aagactaccc
    21841 agaaccgcga taatgttgcg aattttcata actgtaactg ctccctaatt atgagcattt
    21901 caacatagag atcataatgc ccgatatttt acctcttttg tgattagatt gcaaatttcc
    21961 taatttattt tatcagcgaa tagataaaaa ttacgttgta aaaagtattg aaaattacca
    22021 gatttggtct atgaatcaag aggatattat cattcagaaa tgcaaaatct gatttacgtc
    22081 gggcgctggc tggtcgggtt ggtttacttt ctgttgttag gaatgactct ccggttcggg
    22141 cttatttttt ggaaacctgc cgaaatctat gttctggtac agcaaatctg ccgcgccgct
    22201 ctacgaacga tcggcatccg cataaaagtc agtggtttgg atcgctttga tcatcagcaa
    22261 tcttacttga ttatttgtaa tcatgagagt ctcctggatg ctttcatctg cccggcttat
    22321 atcccgatgt attttactac tatcgagtta gccgaccatt tcaagtggcc actctggggc
    22381 tggatgatcc gtaattgggg gaatatcccg ctccaacacg gtcagctcaa atcctcacta
    22441 acaagtttag accaagccgc taccttgctt aagaatggca cctcgattct gatttttccc
    22501 gaaggtagcc gcactaccga tggtaaattg ctttccttta aaaaaggtgc tttttatctg
    22561 gcatatcgcg ctaaggcaga cattttgccg ctggctatta acgggctctg gcgggctaaa
    22621 actcgtggcg attggcgctt acgttccgcc aatgtcagcc tgaatttcgg tgagccgatt
    22681 cgctactcta cctaccaaca cctttcgccc gatgaactgc gcaatctctg ttttgaaaca
    22741 atccagcaat tgaagagtcc ggtagcatcg aatgttttat cgtcttaaaa gtcttaaata
    22801 cgacttgaga ttaacataaa atttcttata ttaccactat gagaaaaaca gacttttccc
    22861 cattacctga gcgtaaacaa ctgctttacg aacagatggc gcgcagttat cgaactcaag
    22921 aacggcagaa aaatacgccg tgggcccctt ttgagaaacg attaatcgaa agtaaaattg
    22981 ccgtaatttc gattgctggc gcttatctca acggccagaa gccatttact aatcgcgaga
    23041 ctgagagcga ctaccaatgc cgcgagattc ctctcaattt taatcgcgaa gacctgcgtt
    23101 ttttcgccac tgattgggaa cccagcgaag ctctggctga ttataatgtc gtcttgccga
    23161 ttgaccgtct cgtgctttta caaaaagagg gtatgatagg caaaatacat gagacaatca
    23221 tcagtatcgc cggctttacc aagagcataa aaatgttcgg gaaattcctg accaaaactt
    23281 acgataccct gaaaaaagcc gaggttgatg gcgtcttaat tctgccttgt tcagctcaga
    23341 catcggccgc cgcttgccag gttgctaatt atttggaaaa gaaagaactg ccgacagtta
    23401 tactgaccac cttctacgaa caggctttag ccttggcgcc tccccgctgt gcttttatca
    23461 attttccttt tggtcgccca tttggcaaag ccaatcatgt taccttgcat acggcgattc
    23521 tgcgagatac cctccgtctt tttgagaaag ccaaaactcc aggcgaagtg atctgtctaa
    23581 gttatatctg gtcattcgga acagtaccga attggtaata cttttggagg tgatttatga
    23641 atttactgga caatctaact tggctaggac attcatcttt ccggattgcc ggaaaaaaga
    23701 taatctatat tgatcccttt caggttaaag ccgggatacc ggctgacctt atcctcgtta
    23761 cgcacgatca ctacgatcat tttgatagaa attcaattac gaatattaag aaaaacgaca
    23821 ccataattgt tgcgcctgcc agtgtttgcc gacaaattag cggcgacacc cgaccggtcc
    23881 gaccaggtgc gcagatgaac ttcgattcgg ttactataaa agctgtcccc gcctataaca
    23941 ttggtaaaaa atttcacccc cgcgaaaatc agttcgtcgg ttacataata cacgccgaca
    24001 acattattta ttatcatgca ggagataccg acttgattcc cgagatgcaa gatattcgcg
    24061 ccgatgtagc tcttctgccg attggtggga cttatacgat ggatgccatc gaagccgccc
    24121 aagccaccaa aataatccac ccgcaggtcg ccgtaccgat gcactggggt accgtctccg
    24181 gtagtcgggc tgatgccgaa aaatttgccc aacttgccaa ttgccaagta gttattttaa
    24241 ataaaggaaa gtagttaatt atgtttaaaa aaggtttaat cttgttaatc ctggcttccg
    24301 cagtttttgc ccaatccaat ccgcccgcga ttcgaccgga tagcgttggg atttacttca
    24361 atatgagtat tcaagcttac agtgaacagc aatatgatcg ctatcttgaa tatactcaga
    24421 aacttattca ggtttttcca gacgatttta ccctacagta taatctcgcc agtgcctatg
    24481 ctttaaataa ggacactgtc aatgctatca aaatcctgaa ttcattagta gataaaggct
    24541 tagggttggt agcagaagac gatcccgatt ttgccgattt gaaaaataca ccggaattcc
    24601 gcgctctggt caagaaaatt gaaaaggtta aaaagccggt aaaatcgagt aaaaaagcct
    24661 ttaccgttgc cgaaaaagac ctcattccgc gcggcatcac ctacgactca caaaccaaaa
    24721 acctatatat aagcagtctt tataaatcga aaattgtcca aattgaccgc aatggtaaaa
    24781 ttagcgattt tgttcctgaa cggcaggatg gacttggtcc ggtaaccgcc attcaggctg
    24841 atccccagcg tcggattttg tgggcgctaa gttcatttgg aatccccaat gaaaaaacgc
    24901 cccgggaatt ggctggaacc gccagcgttt acaaatacaa cctaaatgac ggcaaactcc
    24961 ttaagaaata caatttgccc aggcctaatc ggcattttct gactgatctg acagaagacc
    25021 gagctggcaa tctttatatt gccgattccg gtcagcggac tattttccgt ctcgatgccc
    25081 aaaaagatac tctcgaaaaa ttcctcgaat tgtataaata tccgcaattg gctagcttga
    25141 ccattacgcc cgacgataaa catctattcg taacgtatgg cggcggtatt ctgaacattg
    25201 atgtcgccac cggcaaaatc acaccgctaa ccagtccaga ccatattttg ctggtaggtt
    25261 gtgacggcct aaatttttat aacaactcct taatagctat tcaaaccttc ttgaatcgca
    25321 tcgtgcgttt cgaattaagt ccggatttta gcagtgtcac cgattacaaa atcatcgaat
    25381 ccaataatcc cgatttcaac attccatccg gaggagcaat tgacggcaag tatttttact
    25441 acgttgctaa cagccaaatc aatagcctcg accgttcggg caacgtgatg acacccgagc
    25501 agttgaagga catcctgatt ttcaaagcca aactgtagct ggcaaatcct ttattcgcta
    25561 tgaaggaact tgtcagtttg gctgaatcag ccgcccgagc cgccggtgct atactgcgga
    25621 gttactttca gcaggaatat acaattaccc acaaaggtct tgacgatccg gtcacaaatg
    25681 ctgatactga agccgatgcc ttcctgaaag aaaaattact tagcgcttgt ccggattacg
    25741 gctggttgtc tgaggaaagc gctgattccg ccgaacgttt aacgaaatcg cgagtttgga
    25801 tcgttgatcc gctggacggc acaaaggaat tcattgccgg gcgaccggaa tttatggttt
    25861 caatcggatt agctgaaaat gggcaaccgg ttttgggagt tttatataat ccaattaacg
    25921 acgaactttt ctcggcttat aaaagacaag gcgctttcct gaacggtagc cggattttta
    25981 gtactaagac aaaaactctc agtgaggcgc gtttgatcgt cagtcgctcc gagctaactg
    26041 aaaatctctg gcaaccctat cgtcattttt tcaaaaccct gcaaccgtgt ggtagtgtcg
    26101 cttataaatt ggcactggtc gccagcggga aagccgatat ccatatcagc ctgaaaccga
    26161 aaaatgaatg ggacatctgc gccgcccatt gcctactggc tgaagccggt gccgttttga
    26221 tcggtcgcga tggaatgcca atattctaca atcttaagta cccgattatc ccaactgggg
    26281 taattgccgg gaataaaaag ctcgttgtgg caggacaaaa aattacccag cttaaggttt
    26341 gcaattaatg tccagttgtt ctatatttgc cggtcaatta tgatagacat cgaaaaaaca
    26401 gataaaatta tgtcctctaa aaccgataag gtagatttca gcaaaaatct ctcacgtcat
    26461 gagaaggaaa tgggcatcca gatttccgag atggaaaagt acaaatggat atgtagtaag
    26521 gatcagggac gtgacgtcgg taaaatggct tatttcgaat ggactcaaaa atacggtcac
    26581 aaagtccgcg actggttgga gtctttatca gatgaagaga ttgatcggct tttcgccgag
    26641 ttatccgacc aggtcaagaa ttatattgag gaaaaatcac attagccgat ctttatgatc
    26701 gcatcgcaat ccacatacac ttaaccataa aaatagcggg ggaataatgg agtcaaagga
    26761 ttcgactgtt accaaattac cggaaaatgc ttatcgtcct ctcaagccgg gtgaagaata
    26821 tataccggta attcccgatg caaagatcgt cccggaagtt actccctact cgctcgggtg
    26881 gggacttttt tacgctgtgt tattttcgat ggcggcggca tatttgggac taaaaatcgg
    26941 tcaggtattc gaggcggcta ttccgattgc cattctggcg gctgggacat cggttctttt
    27001 aaagcgtaaa aatgccctgc aggaaaacgt tattattcag tccatcggtt ccagttcagg
    27061 ggtaatcgtg gcgggagcga ttttcacaat tccggggctt tatatactcg gcttagatgc
    27121 cagctatctt cagattttct tagcgtctct tttgggtggc tttctgggaa ttctatttct
    27181 gattcctttc cgtaaatatt tcgtcaagga tatgcacggc gaatttccct tcccggaagc
    27241 gacggcgacg acggaagtgc tgattgccgg agagggcggt ggcgctcagg caaaagtgct
    27301 ggtatccgcg gcgattgtcg gtggactata tgacttttgc gtagctggat tcggtttttg
    27361 gaaagaagtg gtctcgacct cggtttttcg ctggggggct ttgctggctg ataaattaaa
    27421 gtgggtttta aagattaata tcggtgcggc ggtaaccggt ctggggtata ttgtgggtct
    27481 gcgctattca gcgatcatcg ccagcggttc atttttagcc tggtgggtaa tcgtgccgat
    27541 agtgagttat gtcgccggac ttggtgccca aactccggat gcgattttct tcgattatgt
    27601 gcggccgatt ggaattgggg cgatagcggc cgccggtatt atcggcattc taaaatcctc
    27661 taaaatcatc ggcggcgctt tcaaattagc cggtaaagaa ctattcggcg gctctaaggg
    27721 cggtactgtt gaagaaactt cccgtaccca gaaagacatc ccgatgaaat gggtcacggt
    27781 ttttttggtt ctgacattga tctccatctt tttcttcttt ttattcggtg tccggattac
    27841 gctgaaacag gcgatcgtcg gcttactaat cgtggcggtg atctcatttc tattcactac
    27901 ggttgccgcc cgggcgattg ccatagtcgg tactaacccg gtttccggta tgactcttat
    27961 gacgcttttg ctgtcatcat ttattttagt aaaagtcggt ctgaccggcg atcatggcat
    28021 gctggcggcc ctcttgatcg gcggggtggt gtgcacggct ttatctatgg cgggtggctt
    28081 tatcactgac ttgaagattg gttactggct cggtactacg ccaatcaagc aggagagtta
    28141 taaattcctc ggttcgcttt tttcggcggc ttcggtcgga ttggtaattt tattacttaa
    28201 taaaactttt ggttttggtg gcgattcacc gcttcaagcc ccacaggctt ccgcgatggc
    28261 ggccgttatt aaaccattga tgaccgggga gcaagcacct tgggttcttt atttagccgg
    28321 tgtatttttg gcaattatcc tggaaattat tcgtgtgccg tcgttagctt tcgctctcgg
    28381 aatttatttg ccgattcagc ttaatacacc cctgctgttc ggcggcttgg ttgcctattt
    28441 agtttctaaa agcacgaaga atgaagccct cgccaaggcg cgtagtgacc gcggtactct
    28501 catcgcttcc ggatttattg ctggtggcgc cattatggga gtcgtttcag cctttatcat
    28561 ttttattggg caaaatattt tcgg
//
LOCUS       DAOMJU010000019        28461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1491_length_28461_cov_15.374886, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000019 DAOMJU010000000
VERSION     DAOMJU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 28461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28461
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1491_length_28461_cov_15.374886"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLO58_03180"
     CDS             <1..407
                     /locus_tag="PLO58_03180"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HQO73825.1"
                     /translation="SYDNQNHPKVLVGRLKPKYRHQILPALKEAQKLIGKPYDNFFDI
                     ENDAYYCSEVIYKAFLKSTDNQSLFKLQPMTFVDPSTGETFPIWQEYFAELGVAIPEG
                     KLGINPGEISRSKAIDIIYNYGIPNGWKKLNK"
     gene            427..1410
                     /locus_tag="PLO58_03185"
     CDS             427..1410
                     /locus_tag="PLO58_03185"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HQO73826.1"
                     /translation="MKQIRIGIVGYGNLGRGVEQAVTQNPDIELVAIFTRRDPAKIIT
                     ASESTKVLSANELASMQSAIDVMILCGGSATDLPKQGPAFASLFNTVDSFDTHAKIPE
                     YFAKVDKAARDNGHLAIISVGWDPGLFSINRLYAEAILPNGNGYTFWGKGVSQGHSDA
                     IRRIKGVKNAIQYTIPIEESLVAVRSGINPQLTTRQMHRRECFVVLEENADKDYIENT
                     IKNMPNYFIENETVVHFISEDEFKKNHRKMNHGGFVIRSGHTGAGNKHIIEYSLKLDS
                     NPEFTGNVLVCYARAAYRLAQSGEIGARTAFDIPPALLSPKSGEQLRNELL"
     gene            1483..2520
                     /locus_tag="PLO58_03190"
     CDS             1483..2520
                     /locus_tag="PLO58_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQO73827.1"
                     /translation="MKAVSLTGIRQFTIQQVADPTIVNDTDVLIRVKMVGVCGSDIHY
                     YKNGKIGSQVIQFPFIIGHEATGIVEKVGNKVTRVKIGQRIAIDPTVYCANCDQCRSG
                     RENTCRNLKFLGTPKQLNGALCEYIVLPENCCYPISDKMTFEQAVLSEPLAIAIYSVA
                     RSRLPDNANVAILGVGPIGMSVFCILRIKNVGNIYVTDKIQERLDFAQQLNPEWRGNP
                     DLTNVVSEINKREPLQLDVVYECSGDTTAISQAVQLLKPGGILVLVGIPEMDEISFPI
                     HELRRREITIINVRRQVRCTQKAIDLLESHRVNMNAMATHHFPLEKTAEAFELVANYR
                     EGVMKALISID"
     gene            2606..3955
                     /locus_tag="PLO58_03195"
     CDS             2606..3955
                     /locus_tag="PLO58_03195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQO73828.1"
                     /translation="MQKITRKNLTCSNLLKLILILLIIGQFSPILVARDTSRAAYYPD
                     PTQYPDHIILSWSDDPASTQSVTWRTDTSIKSAYGEIALADASPNFVYQAKRIPAKTE
                     LLNFDGKSANYHSVTFTGLQSNTLYIYRVGSDKRWSEWFQFRTASKKSEPFRFIYLGD
                     GQEDILFLWSRAIRTAYKTAPDTRFIIHGGDLVNNARDYPALNEWFTAAGWINATVPI
                     MPAIGNHEYYDGNWGEQFFMPYWRPQFTLPLNGIEELPETNYYFDFQGLRVIVLNSNF
                     ELEKQAQWLEEILKNNPNKWTVVAFHHPIYCGAEGRDYQELRDTWNPIFKKYKVDLIL
                     NGHDHTYARGNNPERLTIRPNDPTGPVYVVSVSGPKLYNINKIRWMDRAAENTQLYQI
                     ITINDNILDYKAYTVTEELYDAFQIIEKFKGHKVFIDLQPNNFPERMYKNTLNAPNN"
     gene            3966..4205
                     /locus_tag="PLO58_03200"
     CDS             3966..4205
                     /locus_tag="PLO58_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
                     /protein_id="HQO73829.1"
                     /translation="MKYDKDEVDNMILALLFLTSSSDQYGTKAWKGFDFEALDRLYQK
                     GLISDPKDKSATVSLSEKGAKLSKELFFKYFVIKD"
     gene            4509..5564
                     /locus_tag="PLO58_03205"
     CDS             4509..5564
                     /locus_tag="PLO58_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73830.1"
                     /translation="MLIFVINASGDDYEYYFYNPDLDYGSESIFNPISLFINGSFDIL
                     RNGAHNKNIFDQPYKTGFNNVVDNVLHPIDNIRNYGVKNFLNREIFNLEVNKNESQFL
                     PNIGLHLIGNGMQYRKLAEWYDYHEYPNPNLLSIITTISYQLVNEIVENGSYSGTNVD
                     PISDMLIFNPLGILLFSTKAGQEFFSTKIPIYDWSLNPYFNPNNYFLENAGQQYATKI
                     KLKTTDKNALFGYWGVVSTFGISHTINETDAISFSAGAVVAKLTPNEEYDSRFIIPNL
                     DYAVAIFYDRNNSLLGSLTLFGPKFYNLRLEIFPGLIGFKNYKPGYFIGIGEMDNFQI
                     GITFPPIPIGIIGKVTN"
     gene            5577..6590
                     /gene="galE"
                     /locus_tag="PLO58_03210"
     CDS             5577..6590
                     /gene="galE"
                     /locus_tag="PLO58_03210"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQO73831.1"
                     /translation="MKILLTGGAGYIGSHTLVELLNQNHEITVIDNLSNSKIESIKRV
                     KTITGKELIFNKIDLCNIIELEKVFSSNQFEAVIHFAGLKAVGESVQKPLLYYYNNIT
                     GTINLCQMMIKYDVKNIVFSSSATVYGNPTSVPITEDFPIKPANPYGNTKYIIETILK
                     DIQIANPNWNVIILRYFNPVGAHESGLIGEDPNGIPNNLMPYISQVAVGRLPYLKVYG
                     NDYPTKDGTGIRDYIHVVDLAQGHLKALDKLSTKPGYCIYNLGTGQGYSVLDVITTFE
                     KWSQRKIPYKIVGRRPGDIAECYADPSKANRELAWKAERNLDDMCRDTWNWQKKNPNG
                     YSD"
     gene            complement(6625..8859)
                     /gene="purL"
                     /locus_tag="PLO58_03215"
     CDS             complement(6625..8859)
                     /gene="purL"
                     /locus_tag="PLO58_03215"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HQO73832.1"
                     /translation="MASQIEPEVNLQTAIQHGLTEEEWNKILKILGRTPTYTELGIFS
                     VMWSEHCSYKNSIALLKTLPRSGKHLLVNAGEENAGLVDIGDGLAICFKIESHNHPSA
                     VEPFQGAATGVGGILRDIFTMGARPIASLDSLRFGPLEAPRNRYLFDNVVKGIAHYGN
                     CVGVPTVAGEIYFEDCYTENPLVNVMTIGLVKHKNVISAVAKGEGNTIMIVGSSTGRD
                     GIHGATFASVELTEESENRRSSVQVGDPFTEKLLLEATLELAKQDFLIGIQDLGAAGI
                     ACSTSEMAVRGKSGMIINLDSVPLREPGMSPYEIMLSESQERMLVVIRKGFEEETKAI
                     FEKWDLNCVKIGKVVNSGNLDIFMNNKKVASLPAWHLVLGGGAPVYERETKEPAYLKK
                     VQGRNLKKIPEPKDYNKVLLRILSSPNIADKSWVYRQYDYSVRSNTVVKPGGSAAVIR
                     IKGTRKALAIKTDGNGRYTYLNPYKGGAIAVAESARNVVCSGARPIAITNCLNFGNPY
                     DPEIYYQFKMAVQGIGDACRVFDTPVTGGNVSFYNESPKGAIFPTPVIGMVGLIEDLK
                     NVTTPWFKNDGDFIILLGALKGELGGSEYLKVVHNIIAGDAPDIDLTFEKRIFNATLE
                     AIQKGIVNSAIDISDGGLAVALAEACISNPEKPLGASVYISRKLRNDELFFGESQSVI
                     LISISEKYLLDIERIASKNIIPSVTIGRVKDNGRLTINNIIDLPLTELQKAYTRTIPG
                     FMDI"
     gene            complement(8886..10124)
                     /gene="lysA"
                     /locus_tag="PLO58_03220"
     CDS             complement(8886..10124)
                     /gene="lysA"
                     /locus_tag="PLO58_03220"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQO73833.1"
                     /translation="MKINGLNVSDLADEFGTPLYVYDFDRIKDNAQRLRRAFDSDEIE
                     TAFFYAVKANSHPAIVQLLKDCGYGCDCVSPGEIEIALKTGVKPSDIIFAGNYESMED
                     FNVAVEAGVNIILDDITSLDRLLRIHCPEVISFRINPGKGRGKYEQITTGGEKAKFGV
                     PHEKAWLAYEKACQVGIERYGAQMMTGSGILDEEHFPRMLELLLNILGQIHQKLGITF
                     EYVDMGGGFGIPYYGDEHELDIQWVGRECLKVLKAKSESLSIGKPALHLEPGRYLVGD
                     AGYLVTRVLGIKESYQKFAGLDAGFNTLIRSALYKAQHPVIVDGKEDEKELYPINLCG
                     QICENTDIFTVGRPLPKLEEGDLLIFTQAGAYGSTMSMTYNHRLRPAEVAITNGKAIE
                     ITRRETMSDYWSRITYPILL"
     gene            complement(10138..11967)
                     /gene="uvrC"
                     /locus_tag="PLO58_03225"
     CDS             complement(10138..11967)
                     /gene="uvrC"
                     /locus_tag="PLO58_03225"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQO73834.1"
                     /translation="MAERQFPEKLLEKIRTAPTDPGCYIYRNSAGKIIYIGKAKNIRN
                     RVRSYFIGGYKEPKTAALVNNIADVEYILTNSEIEALILENTLIKTHHPKYNIFFRDD
                     KTYPYVRITNEPFPQVFITRSVVRDGSKYFGPYTDSRLLRDTLEIIKNIFPIRSCKHH
                     LNDKTIANGHIKVCLDYHIKKCEGPCQRLVSAEEYNRMINQVIAFLNGRTEGSIEYFE
                     EKMKTAAASLQFETAAAYRDKISILMNYSNRQSVELNDELDRDLIAVLKEAENCCAVI
                     FRVRQGKLIGRDKFLLEGTENYDYPAITHDLLLRYYDNATSIPSEVLLNYLPEEQEML
                     EKWLMSQKGARVSILQPQRGDKARLMKMAIRNAELQISELQMKQSQKTEFVPKSLVAL
                     QNELRLKELPRRIEAFDISNIGGKFAVGSMITFINGVAKSSEYRRFKIKTVTGSNDYA
                     MLAEVVRRRYTRLLREEKPLPDLILIDGGKGQLNAAVTVLQELNLSAIPVIGLAKRLE
                     EIFIPGNDEPILLARNSLALSLLQRVRNEAHRFAITYHRKIRGKGGISTKLDEIPGLS
                     QNVKKALLQHFKTIANIKSASMEDLMNVKGIGPKIAQKIKQYL"
     gene            complement(12047..12229)
                     /locus_tag="PLO58_03230"
     CDS             complement(12047..12229)
                     /locus_tag="PLO58_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73835.1"
                     /translation="MKYCDLTCEYAEFPDKLSDGSATCQTFIAIHCKLLDRLVDKNGI
                     CPAEQSATDKSVNKGR"
     gene            complement(12322..15468)
                     /locus_tag="PLO58_03235"
     CDS             complement(12322..15468)
                     /locus_tag="PLO58_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQO73836.1"
                     /translation="MSLSNLAIKRGITFTMIYLIVIGFGIFGLTQLKLDLYPNITIPV
                     VGVMTQYTGVGPEDIENVITRPLEQTLVAVENVDKIISMSSVGNSLVILQFDWDADID
                     QSEIDVRKNIDLIRDHLPPDASNPITISFDPSMTPVMLISASSGQLGMAELRRVIDDE
                     VRPRIERLKGVASAASGGGLERQIKVLLDPQKVAAYGIPAQQIVQKLQMENVQIPAGV
                     VDDEYQEYSVRTYGEYRTVEQIANTVIGNVSGTPIYLKNVANVIDGYKEQSQIVRNNG
                     EDAVMLFIQKQSDANTVQTARAVAKELPKIMELVGKDVKLEIVFDSSEFITKSLNNLV
                     STGVQAFILVFLVLLFFLRYIRSSLITAISIPVSIIVTFFAMYLLNMTFNVITMAGLA
                     LAIGMLVDNSIVVLENIFRLKEKGLDIRQAADEGANEVSMAITASTLTTIAIFVPVLF
                     LPGISGILFKDLVIVIVSSLTISLLVALTLIPLLSTQWLKGEKKKSKSKVLRRMDSVI
                     DNFLQGLHDRYVRILDYFLSHKKVFLIGLIVIFVITILMFPFLGGEFLPEADRSNIRI
                     SVERETGASLASTDKTFREIEKIIREEVPEAENIQVNFGVGEGVTAIMASSNSGNITV
                     RLPDVEERERSSFEIQDILRERFQKIPGANITIAGGEEATSMLSGGITIKIFGYDRLI
                     ARDLGDQVAEMLKDIKGVVDVTKSYNLPKPEYQIIIDRDKASAFGLSTYQIANIVGTS
                     IRGTRVTRFREGGDEYDVIVQLDEQFRKSQADIENLYITTPTGAQFPLKNIARVLPGA
                     GEESINREDQERRVSVSCSVSGRDIQSVTRDITAGLRNINFPPEFRWEIGGAEEERRE
                     AFLYLYIALAAAVILVYMVMASQFESLLDPFIIIFTIPLAMIGAIWLLFITGTPMSVM
                     ALVGCILLVGIVVNNGIVLVDYINQLRYKHNYHLWIAILAGSKRRLRPVLMTALTTIL
                     AMLPLALGLGTGAELWAPMAIAVIGGLTFATVFTLILIPIIYLFFEQIALKRKIKKGK
                     VEAEPIGRPEGFDINLVK"
     gene            complement(15465..16607)
                     /locus_tag="PLO58_03240"
     CDS             complement(15465..16607)
                     /locus_tag="PLO58_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQO73837.1"
                     /translation="MKLSKNILNLFLVCGIIYYVNSCSKTQTDEQMTENIVPVNVVTA
                     ERGTIEEKINFLGNIKGFREIKIYSLIPNKILKINVDVGDWVNKGDVLAVVESDKIEQ
                     AVKQANAGLEAARAQLRNIETEWNRIKKLYDDNAVSKSQYDAITTQRDAARANVKQLE
                     AAVSTAKKQLEDTQIKAPITGIVSSRLLDVGDQAAPQIPVFTVVEMDDVKITIDVIES
                     QFNKVKIGQKAYINVDTYPDTVFVGEISKISPTLNLMTKTSPAEIIISNPEHKLRPGM
                     FARVDVITDVHKNALIIPRYSIIERTSLEYIGGDISSTKTIVNKYVFVVESGIAREVK
                     IETGIENGRIVEVLQGLNDGDLIVSMGQHNLSDSTKVEIIEKGKAI"
     gene            complement(16622..17968)
                     /locus_tag="PLO58_03245"
     CDS             complement(16622..17968)
                     /locus_tag="PLO58_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQO73838.1"
                     /translation="MKFKLKIGVVFLITIASLFAEELNLDIETAREIALQRNPSLKLA
                     REAVLKSRASVNEVRGSMLPSISAFSQFKHAWEVGGMPTPQEFKDLITLLGGTPQETM
                     SFERENTFVAGLNLSQPVFTGGLILNSYQMSKIGVRITESQLKATEQKILSDVTNAYY
                     GVLFANSASEVAEEALHSAEQNLSQVQKFFDVGKASRFDVLRAEVQVANFKPMVVSAN
                     NAKKLAESNLRLILGIGEDININYLEKLSYKESDLINMTLDELITIALAERSEVIMLE
                     DRKKLAEKQVNLAKAKYSPSLVFGTAYQYQAFRDDFDFTTDDLVKSFNSSISLSIPIF
                     SGFATKAKVQQAKIAVKEVDYQAESAIDGIKLEVKAAYFKMKEANENVQTQEKTIEQA
                     KEALRLAELMYSEGASTQLDVLNANVALNQAKMNYQKSLYEYNVALANLKKAINQL"
     gene            complement(18000..>18479)
                     /locus_tag="PLO58_03250"
     CDS             complement(18000..>18479)
                     /locus_tag="PLO58_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQO73839.1"
                     /translation="ELTKPALYYYFQDKNTLFAEIINAIANYSVQFLTEIYQSDKDPI
                     NKLYDIVIMQVTRLKQNPEITKFLINAPIRNIPNETKVEFYKQMQKNKEMLINIITEG
                     KEKGYLKSDLDVNAFLYCYVGGINQFIIDHIKNGTENLNEQNANVIVNTLLNGARNH"
     assembly_gap    18480..18579
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18580..19870)
                     /gene="glnA"
                     /locus_tag="PLO58_03255"
     CDS             complement(<18580..19870)
                     /gene="glnA"
                     /locus_tag="PLO58_03255"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HQO73840.1"
                     /translation="MTSKEFFKFAKQNDAKMVDLKFTDLLGTWQHCTFPINSWGENTF
                     VEGVGFDGSSIRGWQGIHLSDMLAIPDPDTAVMDRFFKEPTVSVIADIIDPITRENYS
                     RDPRFVARKGEAYLKKTGIADTCYIGPEPEFFIFDEVMYEQNQHTSMYLINSIEGAWN
                     TGRFEEPNLGYKPSFKGGYFPVSPTDTYHDLRTEMALELQKVGIAVEVHHHEVATAGQ
                     SEIDMLYMPLTKMADQFMWFKYIVKNVAKRHNKTVTFMPKPIFQDNGSGMHTHFSLWK
                     NGKPLFADNGYAGLSQIGLYSIGGILKHAPAILAFAAPTTNSYRRLVPGFEAPVNLVM
                     SARNRSAAVRIPMYSNNPKAKRLEFRCPDPTANGYLTWTAMLMAAIDGIKNKIDPGEP
                     LDRDIYEMTPQELEKYPKTPKNLAEAIDALEKDYEFLI"
     gene            complement(20178..20510)
                     /gene="cutA"
                     /locus_tag="PLO58_03260"
     CDS             complement(20178..20510)
                     /gene="cutA"
                     /locus_tag="PLO58_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HQO73841.1"
                     /translation="MKKDAFLQIITTTDSKEKAERIAFILVEKGLAACVQVIGPVTSI
                     YRWEGKIENTIEWICQIKTIKDLYNSVEKEILTHHKYELPEIIAIPILTGLKKYLDWM
                     RMQVTTND"
     gene            20639..20726
                     /locus_tag="PLO58_03265"
     tRNA            20639..20726
                     /locus_tag="PLO58_03265"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20673..20675,aa:Ser,seq:gga)
     assembly_gap    20729..20828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21011..22441)
                     /gene="gatB"
                     /locus_tag="PLO58_03270"
     CDS             complement(21011..22441)
                     /gene="gatB"
                     /locus_tag="PLO58_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQO73842.1"
                     /translation="MSEKFEPVIGLEVHAQLLTDTKIFCSCKNAYGAPPNSLTCPVCL
                     GMPGTLPVLNRKAVEYAIKMGLATDCSITAFSRFARKNYFYPDLPKGYQISQYDEPLC
                     RNGKIKIQVNGVEKNIGITRIHLEEDAGKSIHGFDNTLVDFNRCGVALIEIVSEPDLR
                     SPEEAYAYLIKLKQILEYLEICDCNMEEGSLRCDANISLRPIGEEKFGTKTEMKNMNS
                     FRGVERALRFEIERQKAILEKGKNIIQQTMLWDEAKGEARPMRSKEESHDYRYFPEPD
                     LVPLEVDENWKTKISNDLPELPDQKYFRFLNKYNLRHYDAAILTSDKYLAEYFERVND
                     LINDPQLVSKWIQGEVLRTLSQKNLTSKQIPLAAENLGELLNLIKAGTISANIAKEVF
                     DKMVENNESAKIIVEREKLIQVSDDSELEQIIQTVISENPNEVERYRRGAKNLFAFFM
                     GQVMKKTKGKANPSVANNLLRKALDN"
     assembly_gap    22675..22774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22775..23445)
                     /locus_tag="PLO58_03275"
     CDS             complement(<22775..23445)
                     /locus_tag="PLO58_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQO73843.1"
                     /translation="MLKSRKIAVIAGDGIGPEIIRQVVKIFEVLKNYSQINLAITHAP
                     YSAEYYLNTKVAIPDEYIAELEKNYDAILIGPFGDPRLPDGRYAREIIWGIRNKLDLF
                     FGYQHVKVYDSWMSPLKNMQEGLADFYIIRENLESINPKPGGSYYRDTNKEIAVQSSI
                     FSRQNIDRFIQQAFEFAGKRNYKKVVLAHKNTLMTNLHELWLKIFQEKITAFPEINAD
                     VMNIDA"
     gene            complement(23445..24716)
                     /locus_tag="PLO58_03280"
     CDS             complement(23445..24716)
                     /locus_tag="PLO58_03280"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HQO73844.1"
                     /translation="MSHSLSKTSLLVCGLLTVALAQEPADFRGNVEPEIPNFIYDVSI
                     TPIVTRDSIKIDIIIKVPYSAIQFVKKDTIFIGKYEISVMLLDEKEVNAISKIWTKTL
                     QTKKFEETHSNELYDINKLTYKVKPSDYSLTIGVMDLDTRKTTHRKRELKLKNYYKKS
                     ITLSSINIIEDIVADSSGDSTDVSALTGTLSNKSEKFDISFFVLSNGGKGTIKYSIYN
                     MNKKVVLENKIDHNFKKGVEYQRISIPRQKLSYSKYRLVLTVKIGSEEVTAEKIIQIR
                     WTGMSDMINNLDEAIEQLRYIARGDVISKMKKASGEEKKNLFLEFWRERDPTPGTEEN
                     ELMNEYYRRVAFANQNFSGYLEGWKTDMGMVYILFGPPNDIERHPFEIQTKPYEVWYY
                     YELNRTFIFVDETGFGEYRLITPLDYYGSFY"
     gene            complement(24697..26397)
                     /locus_tag="PLO58_03285"
     CDS             complement(24697..26397)
                     /locus_tag="PLO58_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73845.1"
                     /translation="MQIFKEIRGNFGLIFSICISILFLPQKSPSQDISDRPVRLYQLW
                     DWISYKYSNYPTSLTEGVELVYFGTSGGIYAFHKYGRYWTEPFTTSNGLSDDFITAVL
                     YDETTRYLWAAHNKGVSYLTPSSFRWTNISNADLNLPETNRILRLGTDGNAIWVQAEG
                     DYLLTINKILGFYQAEEESASEAVKWQPTSLDGVPDMRGYTIDQDYQIDGRGLILDQN
                     LREYRVTVFAKINNDIYGGIAGLGLFEGNANIKTLHIHPFGPMQNYINAMAVDDNRIW
                     VGGTRSDLNPIFTKSGISCYNQNDGFWEYFESLFINEFATPVVNDLAYKNNRLWIGTE
                     QGLSIYDQNKNRWKRLSMSSGLADEIITSIALEDTLAWIGTPRGLNLISIPSYKARRQ
                     RLSPRQNILKIFKIAIDSKIIWIATDNGIYSIDKFNHNVEHYNMSGERIGLDDAVAGN
                     VTAIGCGDSVTVFGRYHDLLQFSYKDKQWTNIPLTDQLNEASFFDIAIDGNYMWLGTD
                     RGAFLMRLSDYYCENYTTLDGLAGNNVFKIFIDGDWVWFATDCGLTKYSWRKYVTLTE
                     "
     gene            complement(26398..27495)
                     /gene="dprA"
                     /locus_tag="PLO58_03290"
     CDS             complement(26398..27495)
                     /gene="dprA"
                     /locus_tag="PLO58_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQO73846.1"
                     /translation="MDKLQAILSLMSINGLGSHRILSLYRQYPDIQEVFSVSPKRLIE
                     VPGIDFKIAERIKNNVDHQFVDQQLNWLKNTPYKITTIIDEDYPVRLRNIYDPPVVLF
                     RYGEFLPQDEDAIAIVGTRNPDHYGKEVAALLVSQLVEQNITIVSGFARGIDTIAHKT
                     ALEEGGRTIAVLGNGIERIYPPENRSLRDKLVTQGVYCSEFPFGTKPDAVNFPRRNRI
                     ISGLSLGTVVISAGERSGALLTAYYALDQNREVFAVPGRITDEKSFGTNHLIQKGAKL
                     VTSVGDILDEIDAIRRFPRQPRQLEISFEFENEDEKAIYEALSTDPIHIDDLAQKLGK
                     NTYSILATLLSLELKGAVRQLAGKMFIKAGL"
     gene            complement(27488..28459)
                     /gene="obgE"
                     /locus_tag="PLO58_03295"
     CDS             complement(27488..28459)
                     /gene="obgE"
                     /locus_tag="PLO58_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQO73847.1"
                     /translation="MFVDYTKVSVQSGSGGKGCVAFRREKFVPKGGPSGGDGGRGGDI
                     IVKVDHNLNTLQDIKYHKSYKAQNGEPGQGGLKSGADGAPVIIRVPKGTLIRDELTNE
                     ILADLVEDNQEVVIARGGKGGRGNAHFATPTKQTPRYAEPGLPGEKKSLIFELKLFSD
                     VGLIGLPNAGKSTLISKITAARPKIADYPFTTLTPNLGIVKFGDYKSFLVADIPGLIE
                     GAHQGKGLGFRFLRHLERTKVLAFLIEATDPEPERTYQTLNTELVKYLSTFENKPRFI
                     VITKIDLVQELPTLKFDNLPIVPISAITGEGLELFIQQSLKLLAQNG"
BASE COUNT         8348 a         5841 c         5141 g         8831 t
ORIGIN      
        1 gaagttatga caatcaaaat catcccaaag tcttagtcgg ccgactcaag ccgaaatatc
       61 gccaccaaat tctaccggca ttaaaggaag cccaaaaact tattggtaaa ccttacgaca
      121 attttttcga cattgaaaac gatgcctatt attgctctga agtaatttat aaggcttttc
      181 tcaaatcgac tgataatcag tcacttttta aactgcagcc aatgacattt gttgatccaa
      241 gtacaggaga aacttttccg atatggcaag agtatttcgc cgaactcggt gtagctatcc
      301 cagaaggaaa gttaggtatc aatcctggtg aaatatcgcg ctcaaaagct attgacatca
      361 tatataatta tggaattcca aacggctgga aaaagctcaa caaataaaaa tgaaaggaat
      421 atccctttga aacaaattag aatcggtatc gtcggctatg gaaatcttgg tcgaggagtg
      481 gaacaggctg taactcagaa tccggatatt gaattagtag ccattttcac cagaagagac
      541 ccagctaaaa ttataacggc aagtgaatct actaaggtgc tgagcgccaa tgaattagca
      601 agtatgcaat cagcaattga tgtgatgata ttatgtggtg gttcagccac agatttgccg
      661 aagcaaggac cggctttcgc ttcgttgttt aacacagtag atagcttcga tacgcacgcc
      721 aagattccgg aatatttcgc taaagttgac aaagctgcgc gcgataatgg tcatttggct
      781 attatttcgg taggttggga ccccgggtta ttttcaatca atagattgta tgccgaggca
      841 atcttgccta atggcaacgg atatactttt tgggggaaag gggttagcca gggacattcg
      901 gatgcgattc ggcggattaa aggtgtcaaa aatgctatcc aatacaccat accaatcgag
      961 gaatcacttg tggcagttcg tagtggtatc aatccgcagt tgaccactcg tcaaatgcat
     1021 cgccgagaat gttttgtcgt gcttgaagaa aatgcggata aagattatat cgaaaatact
     1081 ataaaaaata tgcccaacta tttcattgag aatgaaacgg ttgtccattt tatttccgaa
     1141 gatgaattta agaagaatca tagaaaaatg aatcacggtg gatttgtaat tcggagcgga
     1201 cataccggag cagggaataa gcacattatt gaatattcgt tgaagttaga ttctaatcct
     1261 gaatttaccg gaaatgtatt ggtttgttat gcccgtgcgg cttatcgttt agcacagagc
     1321 ggcgaaatag gtgcgagaac tgcgtttgac attccgccgg ctttattgtc acccaagagt
     1381 ggcgaacaac tgcggaacga gttattgtga tcatttttag atcgtttaaa tctggttgga
     1441 taatcataga taaattttat ttttaaaata aatttaatta taatgaaagc agtatcactc
     1501 accggaatca gacaattcac catccaacag gttgccgatc caacaattgt aaatgacacc
     1561 gatgtattaa ttcgagttaa aatggtcgga gtttgtggct cagacatcca ttattataaa
     1621 aatggcaaaa ttggatcgca ggttattcaa tttccattta tcatcgggca tgaagccact
     1681 ggaatcgttg aaaaggttgg gaataaagtt actcgagtca aaatcggtca gcggattgcc
     1741 attgatccga cagtttattg tgctaattgc gaccagtgtc gctcaggtcg tgaaaatacc
     1801 tgccgcaacc tgaaattcct gggtactcca aaacaactaa acggtgcttt atgcgagtat
     1861 atcgtgctcc cggaaaattg ttgctatcca atcagtgata aaatgacctt cgagcaggct
     1921 gttttatccg aaccactggc gattgcgatt tattccgtcg caagatcccg gcttccggat
     1981 aatgctaatg ttgctattct gggagtcgga ccgattggta tgtcagtttt ttgtatcttg
     2041 cgaataaaaa atgtcggtaa tatctatgta actgataaaa ttcaagaacg cctcgatttt
     2101 gcccaacaat taaatcctga gtggcgcggt aatccagatt taaccaatgt agttagcgaa
     2161 attaataaac gagagcctct acaactggac gtcgtttatg aatgtagcgg agatacaaca
     2221 gcaattagtc aggcggttca attgcttaaa ccaggtggaa ttctagtatt ggttggcatc
     2281 cctgaaatgg atgaaatttc atttccaatt catgaacttc gacgtaggga gatcaccata
     2341 attaatgtcc gtcgccaagt ccgttgcact caaaaagcta tcgatttatt agaaagccat
     2401 cgggtaaata tgaatgcaat ggcgacgcat cattttccac ttgaaaaaac tgccgaagca
     2461 ttcgaactgg ttgctaatta tcgggaaggt gtaatgaaag cactaatttc catagattaa
     2521 tgttttttga tttatttaga tgatatttaa ggttatgtaa tataattaaa tgaaagggta
     2581 gaggcacaaa aggagcaatt ttatcatgca gaaaatcacc agaaaaaatt tgacttgcag
     2641 taatctatta aaattgatat tgattttatt gataatcggg caattctctc caattttagt
     2701 tgctcgtgac acatcacggg cggcttatta tcccgatccg actcaatatc ccgaccatat
     2761 aatcctttcc tggagtgatg atcccgccag cactcaatcg gtaacctggc gcacagacac
     2821 cagcattaaa tctgcttatg gtgaaatagc gctagccgat gcatcgccca actttgttta
     2881 tcaagccaag cgaatacctg ccaagaccga gctacttaat ttcgatggaa aaagcgccaa
     2941 ttatcattcg gtaactttta ccggtttgca atcaaataca ttgtatattt accgggttgg
     3001 ttcagacaaa cgatggagtg agtggtttca atttcgcact gcgagtaaaa aatcagaacc
     3061 atttcgattt atttacttag gcgatgggca agaagatata ctttttctat ggtcacgagc
     3121 gatccgcacc gcatacaaaa ctgcgccgga tacgcggttt atcattcatg gcggtgatct
     3181 ggtcaataat gctcgtgact atccggcttt aaatgaatgg ttcacagccg ccggctggat
     3241 caatgccacg gttccgatta tgccagcaat cggtaaccat gaatattatg atggaaattg
     3301 gggcgagcaa ttttttatgc cgtactggcg gccgcaattc actttacctt taaacggaat
     3361 cgaggaactt cctgagacta attactattt cgattttcag ggcttgcgtg tgattgtact
     3421 taattccaat tttgagttag agaaacaggc gcaatggttg gaagaaattt tgaaaaataa
     3481 ccctaataaa tggacggttg tagccttcca tcatcccatt tattgcggag ctgaaggtcg
     3541 tgattatcag gaacttcgtg atacctggaa tccaattttc aaaaaatata aggtagattt
     3601 aattcttaac ggtcatgacc acacttatgc acgcggtaat aatccagagc gtttgactat
     3661 tcgtcctaat gatccaacag gaccggttta tgttgtttca gtcagcggcc caaaattata
     3721 taatattaat aaaatccgct ggatggatcg tgcggcagaa aatactcaac tatatcaaat
     3781 tataaccatt aatgataata ttctcgatta taaagcctat accgttactg aagaacttta
     3841 tgatgccttt caaattatag agaaatttaa aggccacaaa gtttttattg atttacaacc
     3901 gaataatttt ccagagagaa tgtataaaaa tacattaaat gcaccaaata attaggattg
     3961 tataaatgaa atacgacaaa gacgaagttg ataatatgat tttagctctt ttatttctta
     4021 ctagttcatc tgatcaatat ggtactaagg catggaaagg atttgacttt gaggctttgg
     4081 atcgtttata tcaaaaaggt ttaattagtg accctaaaga taaatcagca actgtatcat
     4141 taagtgaaaa aggtgcaaaa ttatctaaag aattgttctt caagtatttc gttattaaag
     4201 attgaaatat atttcaataa ttaattgata ttcagagaat tgttattact aataatagca
     4261 ttttataaaa acacagtcca agaatataat tcattaatag tataacagaa ggtaggaaga
     4321 ggaaaatcaa ttttatccta tatataactt ctttaaattt atctgcaact atacataata
     4381 tatcttatgc gtatattgat tttagggcat ttttagacat ttgtaagtat cattcaattc
     4441 attttgaatt atgccactgt tgattaaatt cccagttata tgatatgtat aaaaaaatta
     4501 ttttgttaat gttaattttt gtaatcaatg ccagcggtga tgattatgaa tattatttct
     4561 acaatcctga ccttgattac ggctctgaat caatttttaa cccaattagt ttgttcatca
     4621 acggttcgtt tgatattttg cgaaatggtg ctcacaataa aaatattttt gaccagccat
     4681 ataaaaccgg ttttaataat gttgttgata atgttttgca tcctatagat aatattagaa
     4741 attatggcgt taaaaatttt ttaaatcgtg aaatatttaa ccttgaagta aataaaaatg
     4801 aatcccaatt tttacccaac atcggtttac atttgattgg caatggtatg caatatcgca
     4861 aattagctga atggtatgat taccatgaat atccaaaccc taatctgtta tcaataataa
     4921 ctacaatatc atatcaattg gtaaatgaaa tcgttgaaaa tggttcatat tcaggcacta
     4981 acgtagatcc catttcagat atgttaatat tcaatccatt aggaatatta cttttcagta
     5041 caaaagctgg acaagaattt ttctcaacta aaataccgat ttacgattgg tcgctgaatc
     5101 cctattttaa tccaaataac tattttttag agaatgccgg ccagcaatat gccacaaaga
     5161 ttaagttaaa aacgactgat aaaaatgcgc tttttggcta ttggggtgtt gtctctactt
     5221 ttggaattag ccataccata aatgaaactg atgcgatatc attctctgcc ggtgctgttg
     5281 tagctaaatt aacgcctaat gaagaatatg atagtcgttt tattattcca aaccttgatt
     5341 acgctgtagc tattttctat gatagaaata attcattact cggatcttta actttatttg
     5401 gtcctaagtt ttataatctt cgtctggaaa tattcccggg cttaatcgga tttaaaaatt
     5461 ataaaccagg ttattttata ggaattggcg agatggataa ttttcagatt ggaatcacat
     5521 ttccacccat tcctattgga ataattggaa aagtcactaa ctaaaatgga catactatga
     5581 aaatattatt aacaggcggc gccgggtata tcggaagcca tacattggtc gaattgttga
     5641 atcaaaacca tgagataacg gttattgata atctttccaa tagcaaaata gagtctataa
     5701 aaagagttaa gactataact ggtaaggaat tgatattcaa taagattgac ctatgtaata
     5761 tcattgaact tgaaaaagta ttttcttcta atcaattcga agcagtaatc cattttgccg
     5821 gtctgaaagc cgttggtgaa tccgtccaaa agcctctgct ttactattat aataacatta
     5881 cgggaacgat taacctctgc cagatgatga ttaaatacga tgtcaagaat atcgtgttca
     5941 gttcttccgc taccgtgtac ggcaatccga catcagttcc aattaccgaa gatttcccga
     6001 tcaagcccgc gaatccttat ggtaatacca aatatattat tgaaacgatt ctaaaagaca
     6061 ttcaaatcgc taatccgaat tggaatgtta ttatattacg gtatttcaat ccagttggtg
     6121 ctcatgaatc cggtttaatc ggcgaagatc ctaatggcat tcctaataat ttaatgcctt
     6181 atatttctca agtcgcggta ggacgtctcc cctatttaaa ggtttatgga aatgactacc
     6241 ccaccaaaga tggcaccggt atccgagatt atattcacgt tgttgattta gcccaaggac
     6301 atttaaaagc attggataaa ttatcgacaa aaccaggtta ttgcatttac aatttaggta
     6361 ctggacaggg atatagtgtg ctcgatgtaa ttacgacttt tgagaaatgg tctcaacgaa
     6421 aaattcctta taaaatcgtt ggcagacgcc cgggagacat tgccgaatgt tatgccgatc
     6481 cgagcaaagc caatcgcgaa cttgcctgga aagcagaacg aaacttagat gacatgtgtc
     6541 gagatacctg gaactggcag aagaaaaatc cgaacggcta ttcagactaa ttttctaatt
     6601 ttactaaaat atttgtggat tctattaaat atccataaaa cctggaatgg ttctggtata
     6661 ggctttctgc agttcagtta aaggtaaatc aataatatta ttaattgtca aacgaccgtt
     6721 gtctttaacg cgtccgattg tcacacttgg gatgatattt ttagaggcaa ttcgttctat
     6781 atcaagtaaa tatttttcac tgatggatat gaggataact gattgacttt caccaaaaaa
     6841 cagttcatca tttctaagtt tgcgcgaaat atagactgag gcgccaagtg gtttttcagg
     6901 attggagatg caagcttctg ccagagccac agccaaaccg ccgtccgata tatctattgc
     6961 tgaattcaca atcccctttt gaattgcttc caaggtagca ttaaagattc ttttttcaaa
     7021 cgttaaatct atatcaggag catcaccagc gataatgtta tgaaccactt ttagatattc
     7081 acttcccccc agttcgcctt ttaaggctcc taataaaatg ataaagtcgc cgtcgttttt
     7141 aaaccagggg gtagtcacgt ttttaagatc ttcaatcaga ccaaccatac caattactgg
     7201 cgtcgggaaa atggctcctt taggagattc gttataaaaa ctgacgttgc cgccagttac
     7261 cggcgtatcg aaaacacgac aggcatcacc gatgccctgc accgccattt taaattgata
     7321 ataaatttct gggtcgtatg gattaccaaa atttagacaa ttggtaatcg caatcggtcg
     7381 cgcaccggaa cagacaacat ttcgagccga ttcggcgacg gcaatcgcac cacctttata
     7441 cgggttgaga taagtataac gaccattccc atcagttttg atagccagtg ctttacgagt
     7501 tcctttaata cgaataactg cggctgaacc accgggcttg acaactgtat ttgatcttac
     7561 ggaataatca tactgccgat aaacccagga tttatccgcg atattcggcg acgataatat
     7621 ccgcagaagc accttgttgt agtctttagg ctcgggtatt tttttcaaat tacgcccctg
     7681 cacttttttc aaataagctg gttctttggt ttctcgctca taaaccggtg ccccgccacc
     7741 taataccaga tgccacgcgg gcagagaagc gaccttttta ttattcataa aaatatccaa
     7801 attaccagaa ttaacaacct taccgatttt gacacagttt aaatcccatt tttcaaaaat
     7861 cgccttagtc tcttcttcaa aaccttttcg aatgacaact aacatccgtt cttgcgattc
     7921 agacaacata atttcatagg ggctcatacc cggttcacgg agaggaacag aatcaaggtt
     7981 aataatcatt ccggatttgc cgcgcactgc catttcggaa gtcgagcaag caattccagc
     8041 cgcacctaaa tcttgaatgc cgattaaaaa atcctgcttg gcaagttcaa gagtagcctc
     8101 cagcaatagt ttttcggtaa agggatctcc aacctgaact gaagaacgcc gattctcaga
     8161 ttcttcagtt aattcaacgg atgcaaaagt tgctccatgg atgccgtccc ttcctgtaga
     8221 agagccgact atcattatgg tatttccttc tcctttggca acagcgctaa tgacattttt
     8281 atgtttgacc aagccaatag tcataacatt aaccaatgga ttttctgtat agcaatcctc
     8341 gaaataaatt tcgccagcta cagtcggcac accgacgcaa ttgccataat gggcgattcc
     8401 ttttaccacg ttatcgaaga gatagcggtt gcgtggtgcc tccaatggcc caaaacgtaa
     8461 cgaatcgagt gaagcgatcg gtcgagcgcc catggtaaaa atatcccgta aaatccctcc
     8521 taccccagtc gccgctccct gaaatggctc aactgctgac ggatgattgt gactctcaat
     8581 tttaaaacaa atcgccagcc catcaccaat atcaactaaa ccagcatttt cttcaccagc
     8641 gttaactaaa aggtgctttc cacttctggg cagagtttta agcaaagcta tcgaattttt
     8701 ataactacaa tgttcactcc acatcactga aaaaataccc agttcggtat aggtaggcgt
     8761 ccgccctaaa atctttaata tcttattcca ctcttcctca gttaaaccgt gttgaatagc
     8821 agtttgcagg ttaacttccg gttcaatctg gctagccatt gattactcca gtaatgtgtt
     8881 aattgtcaaa gaagtatcgg atatgtaatt cgactccaat aatcactcat tgtctcgcgt
     8941 cgagtaattt caatcgcctt gccattagtt atagctactt cagctggacg aagtcggtga
     9001 ttataggtca ttgacatagt acttccgtaa gcaccagcct gagtaaaaat tagcaaatcg
     9061 ccttcttcta acttaggtaa aggccgccct acggtgaaaa tatctgtatt ttcacaaatc
     9121 tgaccacaaa gattaatcgg atagagttct ttttcatctt ctttgccatc gacaataacc
     9181 ggatgctggg ctttataaag tgctgaacga atcaaagtat tgaatccggc atccagtcca
     9241 gcaaatttct gatagctctc cttaattccc agaactcgtg tcaccaagta accggcgtcg
     9301 ccgacgaggt aacgaccggg ttctaaatgt aaagccggtt tgccaattga taaactttca
     9361 gacttggctt taagcacctt gaggcattct cttccaaccc attgaatatc aagttcatgc
     9421 tcgtctccat aataaggtat tccgaatccg ccacccatat caacgtattc aaaagttatc
     9481 ccgagttttt ggtggatttg gcctaagatg ttcagtaata attccagcat tcgcggaaag
     9541 tgttcttcgt caagaatacc ggaaccggtc atcatttgag ccccataccg ttcaatccct
     9601 acctgacagg ctttttcata agcgagccac gctttttcgt ggggcacacc gaatttagcc
     9661 ttctcaccac cggtggtaat ttgttcgtac ttacctctcc ccttccctgg attaatccga
     9721 aaacttatta cctcaggaca atgaattcgt aacaaacgat ccagcgaggt aatatcatcc
     9781 aaaatgatat ttactcccgc ttcgacggca acattgaaat cttccatgct ttcgtaatta
     9841 ccggcaaaga ttatgtccga tggttttact ccagttttca aagcaatttc gatttcacct
     9901 gggctaacgc agtcacagcc ataaccacaa tctttcaaca gttgtacaat tgcgggatgc
     9961 gaattcgctt tcacagcata aaaaaaggct gtttcaattt catcagaatc aaaagctctc
    10021 cgcaaacgct gagcattgtc tttaatacga tcaaaatcat aaacataaag cggagtccca
    10081 aattcatcag ctaaatcgct gacattcaat ccattaatct tcatttaaat tcctatttta
    10141 tagatattgt tttatttttt gagcgatttt tggcccaatc cccttaacat tcatcaagtc
    10201 ttccatactg gcggatttta tattggcgat cgttttaaaa tgttgcagta aagccttttt
    10261 gacattctgg ctcaagcctg ggatttcatc gagtttggta gaaattccgc cttttccacg
    10321 tatttttcga tgataggtga tggcaaagcg atgcgcttca ttccttactc gctgtaaaag
    10381 gctgagtgcc aggctgttgc gtgctaataa tattggttca tcattccccg gtataaatat
    10441 ctcttccagt ctttttgcca gaccaattac aggaattgcg gataaattca attcctgtaa
    10501 tacagtaaca gcggcattca gttgaccttt tccaccatct attagaatca gatccggtag
    10561 aggcttttcc tcacgaagaa gacgtgtgta tcggcgccga acaacttccg ctaacatagc
    10621 ataatcatta cttccggtta cagtttttat tttgaatcgc cggtactcac tgcttttcgc
    10681 gacgccgttg ataaatgtaa tcatagaacc gacggcaaat ttaccgccaa tattagatat
    10741 atcaaaagcc tcaatccgtc tcggtaattc ttttagtcga agttcattct ggagagcaac
    10801 cagcgatttc ggcacgaatt cggttttctg gctctgtttc atttgcaact cactgatttg
    10861 caattcagcg ttgcggatag ccattttcat caatcgagct ttatcgcctc gttgcggctg
    10921 gaggatactg actcgggcac ctttttgaga cataagccat ttttcaagca tttcttgttc
    10981 ttccggtaga tagtttagca atacttccga tgggatactg gttgcattat cataatagcg
    11041 caggagcaag tcgtgagtaa ttgccgggta atcatagttt tcggttcctt ccagtaaaaa
    11101 tttatcgcga ccgatcaact ttccctgcct gacccgaaat atgacagcac aacaattttc
    11161 ggcttctttc agaacggcga ttaagtcgcg atcgagttca tcattcaact cgactgattg
    11221 acgattggaa taattcataa gaatggaaat cttatcacga taagccgcgg ctgtttcaaa
    11281 ttgcagactc gccgcggcag ttttcatttt ttcctcaaaa tattcaatag acccttcggt
    11341 tcgcccgttt aaaaacgcga tcacctggtt gatcatacga ttatattcct cggccgaaac
    11401 tagcctctgg caagggccct cgcatttttt aatatgataa tccaggcaaa ctttgatgtg
    11461 cccgttagca atggttttat cattcaaatg gtgtttgcag cttcgaatag gaaaaatgtt
    11521 tttgatgatt tcgagagtat cacgcaggag acgactatcg gtatatggac cgaagtattt
    11581 cgagccgtct cgcacaacgc tacgagttat aaaaacttgc ggaaaaggtt cattggtaat
    11641 gcgaacgtat gggtaagttt tatcgtcccg aaaaaagatg ttatactttg ggtgatgcgt
    11701 cttaattaga gtattttcga gaatcagagc ttcgatctcc gaattagtca gaatatactc
    11761 gacatcagca atattattaa ccaatgcggc ggttttaggt tctttataac ctccaataaa
    11821 ataagatctt actcgattac gaatgttttt ggcttttcca atataaataa ttttaccggc
    11881 tgaattacgg taaatataac aacctggatc agtcggggca gttcggattt tctcgagaag
    11941 tttttcgggg aattgtcgtt cggccatcag ttaatatcca cagccgtaag ccaaaatcca
    12001 ctccagctat gtaaactttt agtgaataga aataacaaca tctacgtcat cggcctttat
    12061 taactgattt gtcggtcgca ctttgttcag ccggacaaat tccattttta tcaactaatc
    12121 tatcaagaag tttgcaatga attgcaataa aagtctgaca ggttgctgag ccatcagaca
    12181 gtttatccgg aaattccgca tattcacaag ttaaatcaca atacttcatt tattaccctg
    12241 ccaattgtct cgcaaattta aaatgaaaat cgggtagaat caaaccatct cgttgctcta
    12301 cccgattttt actggatttt tttattttac taaattgatg tcaaaacctt cgggtcttcc
    12361 aattggttct gcttcaactt tccccttctt tatctttcgt ttcaaagcaa tttgttcaaa
    12421 gaaaagatat ataatgggta tcaaaattaa ggtgaatacc gtggcaaagg ttaatccgcc
    12481 tatcactgcg atagccatcg gagcccataa ttctgcaccg gtgccgaggc ctaatgccag
    12541 cggcaacatg gccaaaatag tggttagtgc ggtcattaac acaggtctta atcgtctctt
    12601 actccctgcc agaatagcga tccacagatg ataattatgt ttgtacctta gttgatttat
    12661 atagtcaact aatacaattc cattattgac gacgatacct actaatagta tgcatccaac
    12721 caatgccata acactcatgg gtgttccagt tataaataac aaccagattg ctccaatcat
    12781 cgccagtgga atagtaaaaa tgatgataaa cggatctagc aaagattcaa attgggaagc
    12841 cattaccata tagacgagta tcacagccgc agctagagcg atatagagat acaaaaaagc
    12901 ctcgcgcctt tcttcctcgg cgccgcctat ttcccatcta aattcaggtg ggaaattaat
    12961 atttctcaaa ccagcagtta tatctcttgt aacactttgg atgtctctac cggaaactga
    13021 acaagataca ctcactcttc tttcctgatc ttctcggttt attgattcct caccggctcc
    13081 aggcaaaact ctagcaatat tttttaatgg aaattgagcc ccggttggag tagtaatata
    13141 tagattttcg atatcagcct gagattttct gaactgctca tctaattgaa ctattacatc
    13201 atattcatct ccaccttcac gaaatctggt gacccgagtt cctctgatgc tcgtaccgac
    13261 tatattggca atctgataag tactcaaacc aaatgcagag gctttatctc tgtcaattat
    13321 tatctgatac tccggtttag gaaggttgta acttttagta acatcaacaa ctccttttat
    13381 gtctttcagc atttccgcta cctgatcacc taaatccctg gcgattaatc tatcgtaacc
    13441 gaaaatcttg atagtgatac caccacttaa catagaggta gcttcttcac caccagcaat
    13501 ggtaatgtta gcacccggaa tcttttgaaa tctttctctt aatatgtctt gaatttcgaa
    13561 ggaagatctc tctcgctctt caacatcagg taagcgaact gtaatattac cagaattaga
    13621 acttgccata attgcagtaa caccttcgcc aactccgaag ttaacctgaa tattctcagc
    13681 ttccggcacc tcttctctga ttatcttctc tatctctcta aatgtcttgt ccgtcgaggc
    13741 taaagatgcc ccagtttctc tttcgacact tattctaata ttggatctgt ccgcctctgg
    13801 taaaaattca ccacccagaa atggaaacat taaaatagtt atcacaaaaa ttacgatcaa
    13861 tccaattaaa aataccttct tatggctgag aaaataatct aatattctta catatctatc
    13921 atgaagtcct tgaagaaaat tatcaattac ggaatccatt cttctaagga cctttgattt
    13981 tgacttcttt ttctcgccct ttaaccattg cgtagataat aatgggatta aagttaaagc
    14041 aactaacaag gaaatagtca aagatgatac aataacaata actaaatctt taaaaagaat
    14101 accagaaatc cctggtagaa aaagaaccgg aacgaaaatt gctatagttg ttaaagttga
    14161 agccgtgatc gccattgaaa cttcatttgc tccttcatcg gctgcttgtc taatatctag
    14221 ccccttttcc ttaagcctaa atatattttc aagaacgact atcgaattat ctaccagcat
    14281 accgatagca agagccaagc ccgccatagt tatgacgtta aaggtcatat tgagcaaata
    14341 cattgcaaaa aaggtaacta tgatagaaac cggaattgag atcgcagtta tcaaagaact
    14401 tctgatatac ctaagaaaga ataatagaac cagaaaaacc agaataaatg cttggactcc
    14461 ggtactcacc aaattgttta gtgatttcgt aataaattct gaagaatcaa agacaatctc
    14521 aagttttaca tctttaccga ccaattccat tattttcggc aattcttttg ctactgctct
    14581 tgccgtctgc accgtattgg catctgattg tttttggata aaaagcatca ctgcgtcttc
    14641 gccattgttc cgcacaatct ggctttgttc tttataaccg tcaatgacat tcgcaacatt
    14701 ctttaaataa ataggtgtac cactaacatt tccgattact gtattagcta tttgctcaac
    14761 agttctatat tccccataag tccggacaga atattcttgg tattcatcat caacaacacc
    14821 ggccggaatc tggacgtttt ccatttgtag cttttgaaca atttgttggg ctggtattcc
    14881 ataagccgca actttttgcg gatcaagcag gactttaatt tgtcgctcta acccacctcc
    14941 agatgcggct gaagcaacgc cttttagcct ctcaattcgc ggcctaacct cgtcgtctat
    15001 cacccttctt aattccgcca tacccagttg acccgagcta gctgaaatga gcataacggg
    15061 agtcattgac ggatcaaagc tgatggttat gggattcgat gcatctggag ggaggtgatc
    15121 tcgaattaaa tctatatttt ttcggacatc aatctccgat tgatcaatat cggcatccca
    15181 atcgaattgc aatattacca gtgaattacc aacactcgac atcgaaatga ttttatcaac
    15241 gttttcaact gctacgagtg tttgctccag tggacgagtg ataacattct cgatatcttc
    15301 agggcctacg ccggtatatt gggtcattac cccgactact ggtatggtga tattcggata
    15361 taaatctaat tttaattgag taagtccgaa aataccgaac ccaattacaa ttagatagat
    15421 cattgtaaag gtaatacctc gtttaattgc aaggttggat aaactcatat cgctttccct
    15481 ttctcaataa tctcaacttt tgtcgaatct gaaagattat gctgtcccat tgaaacaatt
    15541 aaatctccgt catttaaccc ttgtaatact tctactattc ttccgttttc aatgcctgtt
    15601 tcaattttta cttctcttgc aattcccgat tctacaacaa aaacatattt gtttactata
    15661 gttttagttg aactaatatc gccaccgata tattccagcg aagttctttc aataatagaa
    15721 tacctcggga taattaaggc atttttatga acatcggtaa tgacatcaac tctggcgaac
    15781 attcccggtc tgagtttatg ttcagggttg gaaataatta tttccgctgg tgaggttttc
    15841 gtcataagat tcaacgtggg gctaatttta gaaatttccc cgacaaaaac tgtatccggg
    15901 taggtatcaa cattaatgta agctttttgt ccgattttca ctttattgaa ttgagattca
    15961 atgacgtcta tggttatttt tacatcatcc atttcaacaa cggtgaaaac cggtatttga
    16021 ggcgccgcct gatcaccaac atccaataag cgcgaggaaa caattccggt gatcggggct
    16081 ttgatttggg tatcctctaa ttgtttttta gctgtagaca cagcggcttc cagttgtttt
    16141 acgttcgctc gggcggcatc tcgctgagtc gtaattgcgt cgtactgtga tttactaacg
    16201 gcattatcat catatagttt tttaattcta ttccactctg tttcgatatt tctgagttgc
    16261 gctctggcgg cttccagacc agcgttcgct tgctttactg cctgttctat tttatcactt
    16321 tcaaccacgg ctaaaacgtc gcccttatta acccaatctc ctacatctac atttattttt
    16381 aatatcttat tagggattaa ggaatagatt ttgatttccc tgaaaccttt aatatttcct
    16441 aggaaattga ttttttcttc gatagttcct ctttcagcgg taacgacgtt gaccggaaca
    16501 atgttctctg tcatttgttc gtcggtttgg gttttactac aggaattaac atagtagatt
    16561 atcccacaaa caagaaaaag attcagaata ttttttgata acttcatttt tatctccgtg
    16621 attaaagttg attgatggct ttttttaaat ttgctaaagc tacattgtat tcgtataatg
    16681 atttttgata gttcatctta gcctgattga gagcaacgtt ggcatttaat acatccaatt
    16741 gcgtgctcgc gccctcggaa tacattaatt ctgctaatcg caaggcttct ttagcctgct
    16801 caatggtttt ctcttgagtt tggacatttt cattagcctc tttcattttg aaataagccg
    16861 ccttaacttc cagttttatt ccatcaatag cagattcagc ctgatagtca acttccttaa
    16921 cggctatttt cgcctgctga actttggctt tggtcgcaaa accggaaaaa atcggaatac
    16981 tcaaactgat tgatgaattg aacgatttca ccagatcatc cgtagtaaaa tcaaaatcat
    17041 ccctgaatgc ctgatattga tacgctgtac caaaaacaag cgatggagag tatttagcct
    17101 tagctaaatt aacctgcttt tcggctaatt tttttctatc ttccagcata attacttcag
    17161 accgttctgc cagggcgatt gtgatcaatt catccagcgt catattgatc aaatccgatt
    17221 ctttatatga caatttttct aagtagttta tgttgatatc ttcgcctata cctaggataa
    17281 gcctcaaatt tgattcggct aatttcttag cattgttggc agacactacc atcggtttaa
    17341 aattcgctac ttgaacttcc gctcttaaaa catcaaaacg cgaagctttg ccgacatcaa
    17401 aaaacttctg cacctgcgat agattttgtt ctgcagaatg taaagcctct tccgctactt
    17461 cactggctga gttggcaaat aaaacaccat aataggcgtt agttacatca ctcaatattt
    17521 tctgttcggt agctttgagc tgcgactcgg tgattcttac gccaatcttc gacatctgat
    17581 atgaattcag aattaaacca ccggtaaaaa ctggttgaga taaatttaaa ccagctacaa
    17641 aagtattctc tctttcaaaa gacattgttt cttgaggtgt accaccaaga agcgtaatca
    17701 aatctttgaa ctcttgcggt gttggcattc caccaacttc ccaggcatgc ttaaattggg
    17761 agaaagcact aatggaaggc aacatgctac ccctgacttc gtttacactg gctctggatt
    17821 ttaacacagc ctcgcgggct aattttaaag aaggattacg ctgtaaagct atttcccggg
    17881 cagtctcaat gtcaaggtta agttcttccg caaatagact ggcgatagta attaaaaata
    17941 ctacgccaat ctttaattta aatttcatca aatactcctt tatatattaa taaattagtt
    18001 tagtgattac gtgcaccatt gagaagtgta ttaacgatta cattagcatt ttgttcattt
    18061 aaattttcag tcccattttt aatatgatct ataataaact ggttaattcc tcctacataa
    18121 caatataaaa aggcattgac atccaaatcg gatttgagat aacctttttc cttcccttcg
    18181 gtgataatat taataagcat ttccttattc ttttgcattt gcttataaaa ttcaactttg
    18241 gtttcattgg gaatattacg gatgggtgca ttaattaaga atttggttat ttcaggattc
    18301 tgctttaatc tggtaacttg cataataaca atatcatata atttattgat cgggtcctta
    18361 tcagattggt atatttctgt taaaaattga actgaatagt tggcaatagc attgatgatt
    18421 tcagcaaata gagtattttt atcctggaag taataataaa gggcaggttt ggttaattcn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gattagaaat tcatagtcct
    18601 tttctaaggc atcaatcgcc tctgccaggt ttttcggtgt cttgggatac ttctccagct
    18661 cttgcggtgt catctcgtaa atatcacgat caaggggttc tcctggatca attttatttt
    18721 taattccatc aatcgccgcc ataagcatag cagtccaggt taaataaccg ttagcagttg
    18781 ggtcagggca acggaattct aaacgtttgg ctttaggatt attggagtac attggaatac
    18841 ggactgcggc tgaacgattt ctggcactca tcaccagatt gaccggagct tcaaaaccgg
    18901 gaacgagccg ccgataggaa ttagtcgttg gggcggcaaa agctaaaatc gctggagcat
    18961 gtttcaggat accgccaatt gaatataaac cgatttggct taatccagca tagccattat
    19021 ctgcaaatag tggttttcca tttttccata atgagaagtg ggtgtgcatt ccactaccgt
    19081 tgtcttgaaa aatcggcttg ggcataaagg ttacagtttt attatgacgt tttgccacat
    19141 tttttacgat atatttaaac cacataaact gatcagccat tttagtcaaa ggcatatata
    19201 gcatatctat ttcactttgt ccagcagtgg ctacctcatg atggtgcacc tcgacagcaa
    19261 tacccacctt ttgcaattcg agagccattt cggtccgtag atcatgatac gtatcggttg
    19321 gactgaccgg gaaataacca cctttaaaac ttggtttata tcccagatta ggttcctcaa
    19381 accgtccggt attccaggca ccctcgatgg aattgatcag atacatactg gtatgttgat
    19441 tttgttcata cattacttca tcaaaaatga agaattccgg ctccggtcca atatagcagg
    19501 tatcggcaat gccagttttt ttcaaatatg cctcgccttt ccgggctaca aaccgtggat
    19561 cgcggctata attctctcga gtaattgggt caatgatatc agctataaca ctaacagttg
    19621 gttctttaaa aaaacgatcc atcacagctg tatctggatc aggaatagcc agcatatctg
    19681 acaaatgaat cccctgccaa ccacgaatcg aagagccgtc aaatccaacg ccttcgacaa
    19741 aagtattttc accccaagaa ttgatcggaa aagtacaatg ttgccacgtg ccaagcaggt
    19801 cagtaaactt caaatccacc attttagcat cattttgttt ggcaaattta aaaaattctt
    19861 ttgatgtcat ataaaattcc tttttaatgt tgttatactt ataaattaac ccaaaaaatg
    19921 tgaccctata ttaagtattc aagtaaattc atacaaatta ttttattaat taataaaaaa
    19981 tacatatata ttatgccaat tttaaattta tctttacatt attaattatt agaaatatta
    20041 caaggctaat gttcttaata ttattaatta ctaatgtagc agttacgatt agtacttcaa
    20101 atcgctttta attattaaag taatgaactg cccaagaagc agattattat cagaatgacc
    20161 agagaaaaag tgttaattca atcgtttgtt gtgacttgca tccgcatcca gtccaaatat
    20221 tttttcaatc ctgtcaaaat tggtatggca attatttctg gcaattcgta tttatgatga
    20281 gtgagtattt ccttttcgac agaattgtac aaatctttga tagtttttat ctggcatatc
    20341 cattcaatgg tattttctat tttaccttcc cagcgataga tactggttac cggaccgata
    20401 acctgaacgc aggcggctaa acctttttct accagaataa atgcgattct ttcagctttt
    20461 tctttcgaat cggtggtggt aataatttgt aaaaaagcat cttttttcat tttttaacac
    20521 tcctaataat cataaaataa tttaaatatt aaatactaaa atgataatct accatataat
    20581 cagtctcttg gtttttcttg tcttattggt tcaattaatt taaactatgg cgctttttgg
    20641 agagatgtcc gagtggttta aggagcacgc ttggaaagcg tgtgtaggct tataccctac
    20701 ccggggttcg aatccccgtc tctccgcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20821 nnnnnnnncc acccggggtt ccctgcctgc gtgactgcgt cagcagtcag gcagggaatc
    20881 cccgtctctc cgcagattat ttctgacagg attaacaatg tttattcttc aaacagagtt
    20941 attatgaaaa ttgttattta ttatcaatta attttgatat tcgaaacttc aataattaag
    21001 atataaagca tcaattatcc agggctttcc tcagcagatt attggcgacg cttggattag
    21061 ctttaccttt cgttttcttc attacttgtc ccataaaaaa agcaaaaagg tttttagctc
    21121 ctctgcgata acgctcgact tcgttaggat tctcagaaat taccgtctga atgatctgtt
    21181 ccagttcgga atcatcacta acttgaatca atttttcacg ctcgacgatg attttggccg
    21241 attcattatt ttccaccatt ttgtcgaaga cttctttagc aatattggct gaaatcgtac
    21301 cggcttttat cagattaagt aattcaccaa ggttttccgc cgcgagagga atctgtttcg
    21361 aggtaagatt tttctgcgat aaagtacgta acacttcgcc ttgtatccac ttactaacca
    21421 gctgaggatc gttaattaaa tcatttaccc tctcaaaata ttcagccaga tatttatcac
    21481 ttgttaatat agccgcatca taatgacgca gattatattt atttaagaaa cgaaaatatt
    21541 tctgatcagg caactccggt aaatcattag atatcttcgt cttccaattt tcgtctactt
    21601 caagtgggac aagatcgggt tccggaaaat atctataatc atgagactct tctttagaac
    21661 gcatagggcg ggcttcgcct ttggcttcat cccagagcat cgtttgctga ataatgtttt
    21721 tccctttttc taaaatagct ttttgtctct caatttcaaa tcttaaggct cgttcgactc
    21781 cacggaatga attcatattc ttcatttcgg ttttagtgcc gaatttttcc tctccgatcg
    21841 gtcgcaggga aatattggca tcacatctca aactgccttc ttccatatta caatcacaaa
    21901 tttctaaata ttcaaggatt tgcttcaact taataagata agcgtaagct tcttccggtg
    21961 aacgcaagtc cggttcagag acaatctcga ttagcgctac accacaacga ttgaaatcaa
    22021 ccagagtatt atcaaaacca tgaatagatt tgccggcatc ttcttcgagg tgtattcgtg
    22081 ttataccaat atttttttct actccattta cttggatttt aatctttcca tttcggcaca
    22141 gcggctcgtc atattgtgaa atttgatagc ctttcggcaa atccggataa aagtaatttt
    22201 tacgggcaaa acgcgaaaaa gcagtaatcg aacaatccgt cgccagaccc attttaatgg
    22261 catattcaac ggctttccga ttaagaaccg gtagagtgcc gggcattccc agacaaaccg
    22321 gacaggtcag tgaattaggc ggtgcgccat aggcattctt acaactgcag aatattttcg
    22381 tatctgtcag aagttgggca tggacttcta agccgatgac tggttcaaat ttttcggaca
    22441 ttttcggacc ttctttttaa cgaacacatt gcgtcgtaat ttgatagtaa tttggcaaaa
    22501 agacaaatta tgaatcgcct caattaagaa tttgacggag attaattttg aatcctgctc
    22561 ccgtcttttt aatttccacc caaataattt tgtacttgta taattacgaa tgatttatcc
    22621 aacttactaa ataatgctga atatcaatga ctttaagaca attgtttaaa cgcannnnnn
    22681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcatca atattcatca catcagcatt
    22801 tatctcgggg aaagccgtaa ttttttcctg aaaaattttt aaccataatt cgtgcaagtt
    22861 ggtcattaaa gtatttttat gagccagcac gactttttta taatttcgct tcccggcgaa
    22921 ttcaaatgcc tgctgaataa atcgatcgat attctggcgc gaaaaaatag atgattggac
    22981 cgcgatttcc ttatttgtat cgcgatagta actaccaccc ggtttgggat taatgctttc
    23041 cagattttcg cgaattatat aaaaatcggc taatccttct tgcatattct ttaatgggct
    23101 catccaagaa tcataaacct tgacatgctg ataaccaaaa aataaatcaa gtttattgcg
    23161 aatgccccaa atgatttcgc gtgcgtaacg accgtcaggg aggcgtggat ctccgaatgg
    23221 tcctatcaag attgcatcgt aatttttctc gagttcggca atatattcat ctggaattgc
    23281 aactttggta ttcagataat attccgccga gtaaggcgca tgcgtaattg ccagattaat
    23341 ctggctatag tttttcagca cttcaaatat tttgactact tgtctgataa tttccggacc
    23401 gattccatct ccggcgatga cggcaatttt gcgcgatttt agcattaata aaaactaccg
    23461 taatagtcca agggcgtaat cagacggtat tctccaaaac cggtctcgtc aacgaaaatg
    23521 aacgtccgat ttaattcgta gtaataccag acttcatacg gttttgtctg aatttcaaaa
    23581 ggatggcgtt cgatatcatt tggtggacca aacaagatat aaaccattcc catatcggtt
    23641 ttccagcctt ctaaataacc gctgaaattt tggttagcaa aagcaacgcg gcggtaatat
    23701 tcattcatta attcattttc ttctgttccg ggagttgggt ctctctccct ccaaaattca
    23761 aggaataggt tctttttttc ttctccactg gcttttttca tcttactaat aacatcacca
    23821 cgggcaatat atctgagttg ttcgattgcc tcatcgagat tattgatcat atcagacata
    23881 ccagtccagc ggatttggat tattttttca gccgtgactt cttcagaacc gattttcacc
    23941 gtaagaacca accgatattt actgtaagac aatttctgac gcggaatact gattctctgg
    24001 tattctactc ctttcttaaa attatgatca attttgtttt caaggactac ctttttgttc
    24061 atattataaa tgctatattt aatcgttccc ttccctccat tagataatac gaaaaacgag
    24121 atgtcgaatt tttcgctttt attgcttagt gtaccggtta atgcggaaac atcagtacta
    24181 tctccactac tgtcagcgac aatgtcttcg attatgttga tactgctgag agtaattgat
    24241 tttttatagt aattctttaa ttttaattcc cgtttgcgat gagtagtctt tcgagtatct
    24301 aaatccatta ctccaatcgt taacgaataa tcactcggct ttactttata ggttagttta
    24361 ttaatatcgt aaagttcatt agaatgagtt tcctcgaact tcttggtttg aagggtttta
    24421 gtccagattt tactgatagc attaacttct ttctcatcca gtagcatcac tgatatttca
    24481 tatttgccga tgaatattgt atcctttttg acaaattgga ttgcactgta aggcacttta
    24541 ataattatat caattttaat actatctctg gtcacaatag gcgtgatact aacatcatag
    24601 ataaagttcg gaatttccgg ttccacgttt cctctgaaat cagccggctc ttgggcaaga
    24661 gccaccgtta ataacccaca aactaacaac gatgttttac tcagtgagtg tgacatattt
    24721 tctccagcta tattttgtca gaccgcaatc ggtagcaaac caaacccaat cgccatctat
    24781 gaaaatttta aagacgttgt taccagccag gccatcgaga gtagtataat tctcgcaata
    24841 ataatcagat agacgcatca gaaatgcgcc ccgatcggtt cccagccaca tataattccc
    24901 atcaattgcg atatcgaaaa aactggcttc atttaactga tctgtcagtg gaatattagt
    24961 ccattgtttg tctttatatg aaaattgcag aagatcgtga taacgtccaa aaaccgtcac
    25021 cgaatctccg catccaatag ccgtgacatt accggctact gcatcatcca aacctattct
    25081 ttcaccagac atattataat gttcgacatt atgattgaat ttgtcaatag agtaaatccc
    25141 attatcggtg gcaatccaaa ttatttttga atctatggca attttgaaga tctttaagat
    25201 attctggcgg ggcgacaaac gttgacgacg cgctttataa ctcggaatac tgatgagatt
    25261 cagtccgcgc ggtgtaccaa tccaggcgag agtatcttcg agcgcaatcg aagtaatgat
    25321 ttcatcggct aagccactgg acatagataa tcgcttccag cggtttttat tttgatcgta
    25381 gattgacaaa ccctgctcgg tgccaatcca gagccgatta tttttatagg ctaggtcatt
    25441 gactaccggc gttgcaaatt cattgataaa cagactctca aaatattccc agaaaccatc
    25501 attttgatta taacacgaaa ttcctgattt ggtgaaaatt gggtttaaat cactccgggt
    25561 acctccaacc caaatccgat tatcatccac cgccatagca ttaatgtaat tttgcatcgg
    25621 accgaaagga tggatatgga gagttttaat attggcgttg ccttcaaaaa gtcccaatcc
    25681 ggcaattcca ccataaatgt cattgtttat tttggcaaaa accgttaccc tgtattctcg
    25741 caggttctga tctaaaatta aaccacgccc atcaatttgg taatcctgat ctatggtata
    25801 acccctcata tctggtacgc cgtcaagtga agtcggttgc catttaactg cctctgaggc
    25861 tgattcttcc tctgcttgat aaaatcccaa tatcttattg atagtgagga gataatcacc
    25921 ttcagcctga acccaaattg cgttaccatc cgtaccgagt cgaagaatcc ggttcgtttc
    25981 cggtaaattt aaatcggcgt ttgaaatatt agtccagcga aaactgctgg gagttaaata
    26041 gctgacgcct ttattatgtg ccgcccataa ataacgcgtt gtttcatcat aaaggactgc
    26101 cgttataaag tcatcgctca aaccattgct ggtagtaaac ggctcagtcc agtagcgccc
    26161 gtatttatga aaagcgtaaa taccgccact ggttccgaaa tagacaagtt ctaccccttc
    26221 ggttaagctc gtcggataat tgctatattt atatgaaatc cagtcccaca attgataaag
    26281 tcgaaccggt cggtcagaga tatcttgaga tggacttttt tgtggaagaa acaaaatcga
    26341 aatacagatt gaaaaaatca acccgaaatt gcccctaatt tctttaaata tttgcattta
    26401 taatcctgct ttaatgaaca ttttccccgc caattgccgc acagcaccct ttaattcaag
    26461 cgagagcaaa gtcgccaaaa tcgaatatgt atttttaccg agtttttgag ccagatcatc
    26521 tatgtgaatc ggatcggtcg aaagcgcttc gtaaatcgct ttttcgtctt cattttcaaa
    26581 ttcaaagcta atttcaagct ggcgcggctg acgggggaag cgacgaatag catcaatttc
    26641 atccaatata tcgccgaccg aggtgactaa tttggctcct ttttgaatca ggtgattggt
    26701 gccaaaactc ttttcgtcgg taattctccc gggaacagca aaaacctcgc gattttggtc
    26761 aagtgcataa taagcagtta ataaggcgcc actcctttct ccggcggaaa taacgacagt
    26821 tcctaaactt aatccagaga taatccggtt acgacgtgga aaattgaccg cgtccggttt
    26881 ggtaccgaaa ggaaattccg aacaataaac tccttgtgta accaacttgt cacgcaatga
    26941 ccgattttcc ggtggatata ttcgttcgat gccgttaccc agaaccgcga tagttcgccc
    27001 accttcttcc agagcagttt tgtgggcgat ggtgtcaatt ccgcgcgcaa atccgctaac
    27061 tatcgttata ttttgctcaa ctaattggct aactaataga gccgctactt ctttgccgta
    27121 atgatccgga tttcgagttc ccacaatggc aatcgcatct tcgtcctgcg gcaaaaattc
    27181 accgtatcgg aataaaacca ccggaggatc gtagatattt ctcaatcgaa ctggataatc
    27241 ctcgtcaatt atagtcgtga ttttataagg ggtatttttt agccagttta attgctgatc
    27301 gacaaattgg tgatcaacat tattttttat tcgttccgcg attttgaaat caatgcccgg
    27361 tacctcaatc aatcgtttag gtgaaacgct gaatacttcc tgaatatcag ggtattgccg
    27421 gtataggctc aatatgcgat gagaacccaa tccattaata ctcattaaac tgagaatggc
    27481 ttgcagttta tccattttgc gctagtaatt taagcgattg ttggataaat aactccagcc
    27541 cttcaccagt aatggcagag atgggaacga tcggcaaatt gtcgaatttg agggtgggaa
    27601 gttcttgcac caaatcaatt ttagtaatta cgataaaacg tggtttgttt tcaaaagtgg
    27661 ataagtattt taccaattcg gtattaagcg tttgataagt tcgttcgggc tcaggatctg
    27721 ttgcttcgat taaaaaagcc agaaccttcg tgcgttccag atgtctgagg aatcgaaaac
    27781 ccagcccctt tccctgatga gcgccttcga ttaaaccggg aatatcggca accaagaaac
    27841 ttttataatc accaaatttg acaatgccaa gattgggagt cagagtagta aagggataat
    27901 cggcgatttt gggacgagcg gcagtaattt tcgagattaa agttgactta ccggcatttg
    27961 gcaaacctat caatcccacg tccgagaaaa gttttagttc gaaaatcagg ctttttttct
    28021 caccgggcaa acccggctcg gcataacgag gagtttgttt cgttggcgtg gcgaaatggg
    28081 catttcctcg tccgcctttt ccaccgcggg ctatgactac ttcctgatta tcttcgacca
    28141 gatcagccag aatctcatta gttaattcat cacgtatcaa ggtccctttc ggcacgcgaa
    28201 ttataaccgg cgcaccgtcg gcgccgcttt ttaaaccgcc ttgaccgggt tcgccatttt
    28261 gggctttata acttttatga tatttaatat cctgaagagt attaagatta tgatcaactt
    28321 taacaattat atcgccaccg cgtccaccgt caccgccact gggaccgcct tttggaacga
    28381 acttttcacg acgaaaagcc acacagccct taccaccgct accggattgc acagatactt
    28441 tggtgtaatc tacgaacaaa t
//
LOCUS       DAOMJU010000020        27573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1568_length_27573_cov_20.826877, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000020 DAOMJU010000000
VERSION     DAOMJU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 27573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27573
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1568_length_27573_cov_20.826877"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(473..1123)
                     /gene="phoU"
                     /locus_tag="PLO58_03300"
     CDS             complement(473..1123)
                     /gene="phoU"
                     /locus_tag="PLO58_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQO73848.1"
                     /translation="MLTKKIQELKQAIVEYAALVESMITRSLQALSAKDVNTLKEIIN
                     VDESKANEFDLRMDEACIQTIAQYQPRARDLRTIIMILKMSNDLERLGDGAVHICRDA
                     LYLIDRPSFKPIPLLNHLAQESLGMLHDSIDAFINNDAESAQEICKRDDVVDDLRDKI
                     IAEMTQLMSKQPATVPIALRVMDISRKLERAADLSTNICEDIIFIVQGRVIKHGKN"
     gene            complement(1147..1914)
                     /gene="pstB"
                     /locus_tag="PLO58_03305"
     CDS             complement(1147..1914)
                     /gene="pstB"
                     /locus_tag="PLO58_03305"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQO73849.1"
                     /translation="MNLSKEIRIQAENVNFYYGDKQTLKNVNMSIFQNRVTAIIGPSG
                     CGKSTFIRLLNRMNDLIPNTRVEGRILLDNHNIYDPGVDVVTLRRKVGMVFQKPNPFP
                     KSIRDNLSYGLKINGIKSTDSIENTIRRSLEEAALWDEVKDRLNESALRLSGGQQQRL
                     CIARCLAVEPEVILFDEPCASLDPISTKRIEELIEQLKQRYTIVIVTHNMQQAARVSD
                     YTAFMYLGELIEFGPTEKIFTIPENKHTEDYISGKFG"
     gene            complement(1901..2755)
                     /gene="pstA"
                     /locus_tag="PLO58_03310"
     CDS             complement(1901..2755)
                     /gene="pstA"
                     /locus_tag="PLO58_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQO73850.1"
                     /translation="MKRNSRIQYFGFGFLYLAIFIAVLTLGAIIYFITVNGIKVINWE
                     FLTQVPRRAMTKGGIAPALVGTFYLTIGAIIFALPMGLACAIYLTEYSPKSVVINVIR
                     MSINNLAGVPSVVFGLFGLTVFVKFFGFGVSILSGSLTLGIMILPGIISAAQEALIAV
                     PQSLREASLALGATQWQTIRKIVLPSAMPGILTGVILSVGRAAGETAPIMFTAVTFYT
                     RGFPNSIMDEVMALPYHIYALMTEGTFPERQTMIAYGCALILLFLVLIVSGLAIYLRQ
                     KQRKYELV"
     gene            complement(2752..3636)
                     /gene="pstC"
                     /locus_tag="PLO58_03315"
     CDS             complement(2752..3636)
                     /gene="pstC"
                     /locus_tag="PLO58_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQO73851.1"
                     /translation="MKWNSEKVVKPVFGGLAFASLVFLLGIILILFQQGLPIFRIVNL
                     FEFIGGKAWYPTAEPAEFGILPLILGTLWITLGAMLFCIPVGIGSALFLHEIAGFRLR
                     SILKPIIEILAGIPSIVYGFFGMVIIAPFLQNLLDIPIGQCALTASLILGVMAVPTVC
                     SLAEDALNFVPKSFREASYALGANRWQTLTRVIIPAAGSGISTAIILGMSRVVGETMT
                     VLMVAGGAAVIPKSYLDPVRPMTATIAAEMGEAVVGSPHYHALFAIGLVLFLITMAFN
                     IIAEIISRNYRLKLGLGR"
     gene            complement(3718..4530)
                     /locus_tag="PLO58_03320"
     CDS             complement(3718..4530)
                     /locus_tag="PLO58_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQO73852.1"
                     /translation="MKKLFNLSILILAIASLLTAGEAVKIRMEGSTTVLPIAQLAAEE
                     FMIANPDVTISVQGGGSGVGIASLIDKTCDIATSSRAMKDEEIKNAVAKGVNPVAHIV
                     AMDGIAVILHPSNKIKNLTVEQIRKIYTGKISNWKEVGGEDKKIVVLSRDTASGTFEA
                     FSKLALNDEKVRKDALMNASNQAIATTVEKTPGAIGYVGIGYISSKVKAVTVENVTCT
                     KKNILTKTYPLSRPLFMYTNGKPKGVVKDFIDFILSEEGQELVETAGYVGLK"
     gene            complement(4549..5787)
                     /locus_tag="PLO58_03325"
     CDS             complement(4549..5787)
                     /locus_tag="PLO58_03325"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HQO73853.1"
                     /translation="MKLRERKINIILALLGFCALTLNAGELDRLVNELVEKGVLDAGK
                     AQEILTLTEEEMRAEMNAGKSATLPKWIQNMGLKGDFRFRNQWEATEEDSVSRMRQRI
                     RFRLNGEAVVTSGFKVGFGLATGSSDPRSTNQTLENSFETKPIMLDYAYGSYTFPKYA
                     NVLLGKFYSNLGFWTPTDYMWDSDITVEGVAAQFNHKMIFFNTGIYFLDEKGKVENVI
                     ADNPRMLILQPGFNLAKEGIYSLKAAATYYNFNKTKNMAFDHAKWSNSYITINDSKYL
                     ALGYNNFNFATEVALYNKIFPYIGVCFEINQNLQAESDTAKNGIACGLTLGSEKIKDK
                     GQWQVKYVYRQLEKDAWLDFLPDSDTYGGKTDIKGSELIAQYGLNKNIVLALDYYKIE
                     NLHGAIKPETVLQTDIQFKF"
     assembly_gap    5973..6072
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6246..6788)
                     /locus_tag="PLO58_03330"
     CDS             complement(6246..6788)
                     /locus_tag="PLO58_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HQO73854.1"
                     /translation="MKVQLKKEIEQIVHLQDYAGKPTIEGVKVITLRRHNDDGGSFTE
                     LARITNGDFSGVENFKIAQINFSEMEPQSVKAFHVHRQQTDIWFVPPDSKILLILIDL
                     REDSATNGQRMRLVIGDDNSQLIVIPPGVAHGCKNLGLKKAHLIYFMNKQFSPEPSEC
                     DEWRLPWDYFGGEIWEVNKG"
     gene            7079..7453
                     /locus_tag="PLO58_03335"
     CDS             7079..7453
                     /locus_tag="PLO58_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73855.1"
                     /translation="MAITVFIISLFIGIFVLNLLIFKYLKGSAPQPFFSVTYRPKPLA
                     AFDRYTLKVVALTTLIIIIFMLIAIALQSGFVIPVIHPIALILFIFITGVGFVYSFRS
                     EGNRSKSVQKFSTGETDGEEQK"
     gene            7450..>8093
                     /locus_tag="PLO58_03340"
     CDS             7450..>8093
                     /locus_tag="PLO58_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73856.1"
                     /translation="MKSENVGNWQTLRFIFPDNAAISFDARIADSVIKELALDTGQPV
                     FKLRCSINELYQPNTQIFTSGFPYNDLWFRYAQILVIEKHLKLQVPVGIELIRVIFCE
                     FLRIAEYIFNLATLCLNGGLNDIFSGLMQQYESIQQYFEQILSGTNPMDFIIGHKTPE
                     DLTVGFTEMTLNLSHQLQIQLRKSLPHLLNNRLILSRFSKIADQTADNSAILND"
     assembly_gap    8094..8193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8194..8645
                     /locus_tag="PLO58_03345"
     CDS             <8194..8645
                     /locus_tag="PLO58_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73857.1"
                     /translation="FSKIADQTADNSAILNDKLEFDQMRFTSSKKQNNVCWQLLKIRC
                     DDLFRSIGLIRLSLTKFVYLDRSPQTLPSLTSLPKGNSSVNIETPWGKLSCQMNCPGD
                     RNPCDFILSNPLHKIQLLLCKMAVGLELDELPLLIASLDLRPKIDPR"
     gene            8872..9654
                     /locus_tag="PLO58_03350"
     CDS             8872..9654
                     /locus_tag="PLO58_03350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQO73858.1"
                     /translation="MTTQVFLWIETGYLMALWLVLPYQMQIQGNQTLWLFLAIITLPL
                     NIINSIIASEEVKSEIGQAGIFKNGISLLSGFAPLLLSILIPITINRYTSFWEISSQQ
                     SGYRLGFIPNWTIFSSPLFLLIGIIFLINLTMLMRLSDGSYKPLEYEEPAEIYSGRHE
                     SFGILPQFNRSAMLFTLTTLAIFLFFGGWQTPFEPEGQNLASLAGGLIFFVKTILVFS
                     ACLAIKYHLPILTNWQIIKINLRIQIPVLTLIWLGLLIIRWL"
     gene            9654..10142
                     /locus_tag="PLO58_03355"
     CDS             9654..10142
                     /locus_tag="PLO58_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73859.1"
                     /translation="MTNFQWMPVIFWLATFLTLLPAGLAITSTNPKNLILLNMFTQLG
                     IFLLIISLGVGFPAVIFLVLVFPLNIMMLYGHNLFPETNKQTARESRPAIILKTAVCF
                     LLFLLLIIAFYQNHLKNMLVIDNSGKILEGVIPDVSLLLILIGMFIMLILIAISYFLR
                     RK"
     gene            10139..10444
                     /locus_tag="PLO58_03360"
     CDS             10139..10444
                     /locus_tag="PLO58_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73860.1"
                     /translation="MSHLFLYQIVGIGLFIIGLYGLWRNRHCWAVITFLQLMSSGILL
                     SFLAITRTKGAYQSVSELIVIFIFAVLIIEILLAIVFTYKVLMSDSSSNSDDKSNRG"
     gene            10446..12335
                     /locus_tag="PLO58_03365"
     CDS             10446..12335
                     /locus_tag="PLO58_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73861.1"
                     /translation="MNILIILLLLLPICAYLLTRCLPDKCLLNRRGLSILFLLISGLI
                     GLGIFIVLLLINMPDYVQRISWTWANIAGHSLQISLLLNNFLGLLLALWALGSLIFAI
                     LTATDFFSPDHFRENITNLALGTLAVNGLILANDFLSLIFFWALIDLIIYRVVFRKIN
                     KASESYFPDIMVIGGHLLLILVTFSLFKISGTFRFDLIIEEVLSGKVDPQQLILPGSV
                     LILVGILRIYSPFFRYQFNSQTKMSISDEFILFCGLMPSGLFLVLKLFPIMPQIFLTG
                     LMIFSGISVLIFLIIHLAKTGFNQLLHLNTIFLISLLIITIGLGTFPISASLLFILFM
                     TNFMLIILAQDFQPMSDSANFSIALPFRLIAWLSLIGLPISLAFNTRLLLLQASLTLS
                     SNKPIHGTIISALLVLNYFLNVVVVFRIFLKSGWYEPKHRKIDRVTPLTHCIIAGISI
                     VCLYLFFTLPNFNPLTTELRFFPLFSIQGSQPLDYSQFSSNSLIIYSAILLIGLVVGF
                     ITAKYFKAEKFSRWLTEFSLDFRRYSMIPVANFSQVVARNIRHFDTVLFSRVSQSLMQ
                     TAQTAILFINRINAIGAQQITKLKEVPEKYLNRILDGIFNLKFQVLIGIVLSILIITV
                     LICIL"
     gene            12360..13838
                     /locus_tag="PLO58_03370"
     CDS             12360..13838
                     /locus_tag="PLO58_03370"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQO73862.1"
                     /translation="MTEHLLSINLLIALIGLLGIALTKREKGNLIKTIAAIVTGLQFW
                     TIIEILFNFNPMDTALQFSERINWIPAFRIDYYLGVDGINLVFLIVTSLLIFLSVIIS
                     WNKREPLKEYFILLMILDIGLNGIFIAINLFLFLIFLGVTFFAIYLIIGFSNIRGSVN
                     SVNRFGIPFLISYIFILLGVLLLYKSNNLHTLNLTELTVGNAISHSKQMVISIILLIG
                     FAILIPLFPFHSWLIPLIKNGPKEVSLLILGIVTKVGLYGLVRIIAPVMPFATNSLTL
                     ALGIWGLVNIIYGAICALGSDDSNQILGYFTLQQLGFFLIGFATLGENSNISIAVTGL
                     SGSLLIVISHSCLATLLLIIFKNIRAENQNVARPSLPNIAIVTLTLLAGIGMPGFLDF
                     FARFLILLSAFQTQRLLILAIISLLGIFLNFAIYLKLFKKLVFSQQTRDSAKRLDSNE
                     VFASVSLLIVLILCGIFPSFLLNIIQPGITRLLEMFSTAIAL"
     gene            13846..15249
                     /locus_tag="PLO58_03375"
     CDS             13846..15249
                     /locus_tag="PLO58_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQO73863.1"
                     /translation="MSNFQSIIYFIPEIILIIAAIGFLLLTTFFKISDSKKYFLISVS
                     LIISLIATLLSHSADGHSIFNNLLVWDSYGQFIKIFTLIIACVILIIRRKENCNEASP
                     CPVTEFGIGLLLLISVAFVSINANHLVALFISLQILHLAGFFIVQSRNLSPRINTASW
                     HDLMFDIFTSLIMLMGIALIYDTTGTFFIAEIGQILSTVTRMNFPLILGIALLLVGFS
                     FTITGIYRLSWKLEILRTISASDRLLLLTIPQFIETTTLIRLLRVGLGLVGDWRPLTL
                     MIGIFAVLTLLFPIIWLIKLRSAKDIIIVTSIAQSGFGLLALCAGNISGDVAALFYEV
                     VLIFSLIAFTIGEILTINAKSSQLIALASLIGIPLTAGFPAKYLVFISLVQHSPFYYF
                     LIVLGIGSAALMLIGYVRIVRQIITAQGQFFNSPQKGSQLIFFIQILINIILISIGFY
                     WTPLTDYLQSIMTFCVP"
     gene            15338..16867
                     /locus_tag="PLO58_03380"
     CDS             15338..16867
                     /locus_tag="PLO58_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HQO73864.1"
                     /translation="MSPQNKRFFFWFVIAFLIIFIILISISLIHNFNKSKIISLPAQE
                     MRGVWMSRFDYTEPFPSYNPDSMKTYISDSFRRLKDANFNVIFFQVRGNADAFYASRY
                     EPWSNLLTGKLGQNPGWDPLAYAIETAHQLGLELHAWLNVFPAWRGTAPPTTSQPLHP
                     YLAHPDWLICDGNGKKMLLSDGYVSFSPGIPAVHNHLIKVISDIVSRYDIDGIHFDYL
                     RYPEQSELKGYSHDPISWARFQSHEGNPLGLDWTDWQREQINEFVARAYNCVTDIKPE
                     IKVSAAVIGSYKTDHWNGYSAGFQDPRRWLEIGKIDLIIPMTYYHLNHKNFTFNKALK
                     EWQSVIDNPDQIFPALAAYNLSWEEIIEEIHLVRNSHFKGMVFFAASSLDSEKLQSLQ
                     TTEFQYPSIFPCLTWKDSVAPPSPINFAVEKLTSDSLEFIWQIPHQSNDIKKFVIYQS
                     KNLPIDLTKGENILAIVAGSDTSYQTKFNLPDQRRYYYTITSLDAAYNQSEPASVIKI
                     K"
     gene            17084..18034
                     /locus_tag="PLO58_03385"
     CDS             17084..18034
                     /locus_tag="PLO58_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQO73865.1"
                     /translation="MKVKFWGVRGSIPTPPTSEQIRHKFLRIIDNLPTNIRKNPTEIK
                     KYILSLSYLEAGLIGGNTSCVEIRADNKILIFDMGSGLRALGNYLVKNEQNKNGLDLH
                     IFISHTHWDHIMGFPYFTPAFFPNNKITFYSPHPDIKERLEIQQDSRFFPVSLEHMLA
                     KKEFIQLQPKTSITLGKVKISNYPLYHPGGSYGYRIDWDGKSVVYATDSEYKNLDRES
                     TKHYIDFFRGANLLIFDAQYTFEEAIHKEDWGHSSALIAVEFAVEAGVKKLALFHHEP
                     ERDDFEINDILKKSRDFKKINFPNEILDIFLAMEELEITL"
     gene            complement(18306..20267)
                     /locus_tag="PLO58_03390"
     CDS             complement(18306..20267)
                     /locus_tag="PLO58_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQO73866.1"
                     /translation="MRRNFFHNKTKLLFQAIILLLIIILVIIGCTDKAKKADFEAYCP
                     FGGLVSLGSKFHLGSMSCSMNETQVFMGLFLLIGVILIGKLFCGYICPIGTVTEWLNK
                     LFARFKFSIVLKGKADRILRLGKYVLLFFTAYFTATASELWCKKFDPYYAAVTGFGSD
                     TVLLVGILTIFTVVVLSVFIRFFWCKYICPLGALSNIFSNVLITAPILIIYFGLYIFG
                     IKLHILWLLLALCLSGALTEILRFKFFSISPFRININKNTCTTCGNCDDNCPQGIPVS
                     SYEKVTHPDCTLCLDCTKECSSSGSIRLNKGNYGWLPPVALVVLFILALLTARNFEFK
                     TLSERWGNYAELESVQKFEMEGLKSVKCWGSSKSLQNKLMKQPGIVGLDTYVKSHKVV
                     VYYDSTKIDEYGIKKAIFTPYQYKIQKFDEYQPEKIAVFEIPISGLWDVQDNADLIRM
                     LRVNPKIVGFETNFGEPVIARIYVELGKVTPDSIIYLINQKSYEKKLPNGKTEVVKVN
                     FRCEGRGAFVDTLDYVTFRRRLFAGYDQAFNSYEKYRPADLMIFEIGLPDAESIAVRR
                     ALKYLTSHISFFDGVVRVRTDYTDREALLIYFDPTQVNPKDIQTKLNEPMLKILTSDE
                     KIMEMENSFEFREPLRIYQVTPEMYALEK"
     gene            complement(20384..>23850)
                     /locus_tag="PLO58_03395"
     CDS             complement(20384..>23850)
                     /locus_tag="PLO58_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO73867.1"
                     /translation="DGGANWTTVREPDVSANSWNDMEFIDANVGYACGNGGIIYKTTN
                     GGSDWTMIGDTNTYKFDLYSLDVVNANVVYCAGKSGTVLKTTNGSTFNKLSYNFDGDM
                     TASDETRDDLDGGIAFFDENRGVVAVDFNKEATIWWTEDGGTNWHPVKIAAAFPPGVT
                     SYRVYDVAVGGDSTVALACYHRVCLVSTNGGRTFANKGVVQFANVYTSYVEVFDNNTI
                     IYGGGSDNFGAITTNGGTDWTNFSTGTGQRLLSADFIDARTGYWFCEYQQWMKTADGG
                     VNWIPINNWPGISWLGLAFPDEKKIVLTADGGGEMTFSNDWGKTWSYPNNLRTGLDGD
                     IFECEFIDANNGLIGGSGGYILKTTDGGATFTQIDNPMYNNNKAVNAMHYLNSDTVFV
                     GGGSGYICYSFDGGDTWTKATAGSKTVYDIWPLSNNAVVATCASGEYYTSTDLMTFTK
                     LGATSEAKNLRAVEFRNGIGIIPGASGYIYRSTAWDAMPTLVYTASNGAELYDVEFVD
                     DNTVFVVGQYGTILKSEDAGLNWTVETSPTDEVLQKVRYSDNGSVGILWAVGQNGTIL
                     KNVIYSNVLAGDYYIPQGDHEQGFATLGAAVTALNTYGAIAPVTFYIDADLIEPGSEL
                     IIDREDLTAETALTIKPASGKTPSITINDFPTTGNYSGQGFTIENTAYVTIDGSNTSG
                     GTSRDLTIIGNDTDGKCVIGVIDNSDFITVKNVNITFENLLASGSILGVDGYTGVPNS
                     LLIKNCQIGTPEKTVANGVALRGNSPETPCGAVVMGCDIYAAGTGIMADYISKNAYIN
                     NVISIINPLADQTFYSGMYLNGFAAGDTCVVFNNRIVNVEMNTTAPQFAGGIVIGENG
                     GVIDIFNNFIAMNTTNNGSATDNRVYGIAFGSANWSGEANIYHNTIDIAPTNQTGVHA
                     AIGTEIPFSAKLNIYNNILSNRHDAANSFGIHWHNTVSPNSGLASNYNNIYIAGSSAS
                     VGFYGSIPRADLATWRAASGQDANSINKPVNFVSATDLHVTGSSIGDNDLACHRPDWL
                     STDIDGDARPNVTYMGADEGATALSFVGVANGSSGLPTEFSLRQNYPNPFNPTTVIAF
                     ALPKSTQVTLMVYNMLGQPVAVLQNGFLKAGYYHVNFDASNLPSGTYIYRIQAGDYRN
                     VKKMTIVK"
     assembly_gap    23851..23950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23951..24201)
                     /locus_tag="PLO58_03400"
     CDS             complement(<23951..24201)
                     /locus_tag="PLO58_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73868.1"
                     /translation="MKTTLKVTVLLFAVANLLLGGSWQTVKIASTDMSVNKASFIDAN
                     TGWIVYSTTYPSKQMGKVQKTTDGGANWTTVREPDVSANS"
     gene            complement(24288..25751)
                     /locus_tag="PLO58_03405"
     CDS             complement(24288..25751)
                     /locus_tag="PLO58_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73869.1"
                     /translation="MRTNSDFIKKCLFVGVVIGLVFITPLFAIDNPLGREIQPRNLMN
                     QYNLTIYDLSGNPAFYGISYPDNLDFYQMQATNDNNVFRRLYDPRRQEDYALSFHSAK
                     HLSERITLVTGIRFNRTIQRDLYHSLEKNIYDEYIAFLDTTTGTTTYNSPQINFLYDY
                     ALTKRLHLGLEINYGVEQSLKDVYTQCEIIARNTELRGGLSYISKNQKIIGGLLGTYY
                     NAQRKYEAVKYYQDAFVRTLFGYHIYKDETPKSINRKNDEREGGGLALQFCHRDFMLP
                     GLALIITGEYNGRSDNVTAGSPQKPTPRGHWVREIRRGILDLNYSPIESNNSLEFIYE
                     IRSTDDWVKSGNYEVIILDNAELLNVFSLRWQTRLFKRLKMNIGASYASQACDYQEYT
                     IPFQYHETDYHSSAFLDLNYAINQILSVYFLGNLAIDDLYFYWNAERATRYGCEIGIE
                     RLTTFGRFGAGLEYFQTAFDNPDQYNQTIGLKISYWK"
     gene            complement(25748..26866)
                     /locus_tag="PLO58_03410"
     CDS             complement(25748..26866)
                     /locus_tag="PLO58_03410"
                     /inference="COORDINATES: protein motif:HMM:NF027540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HQO73870.1"
                     /translation="MVKKAALLTIILLLNLTNCFHQKPTEFDSTYTLKLVLVYRDSTL
                     GYDERLSKVDVKIHTGDYNLDDYCATTNDSGVVVFENLPWAIYDPQVKAKVELPFIDS
                     FGNRRLDSLISYTLIDTIVAITPSETGIGTDTIYTITANLRPGLKINEIYYTGPENRN
                     NWFYDQFIELYNSSDEVQYLDGMIVCRIYHMLTSVTYAFRFPGTPLTGREYPVQPGEF
                     VVLAQDAVNWLELYSTSVDLSGADWEFVNAMDFGDIDNLNVPNLANIVPGRTVDFMIN
                     LSTNVIALADGSDSVMTDGLDIGTVVDCVEYNSKSPSEQTTKNIDKILDAGWAGVGNQ
                     KYSGQSVERILPGFDTNNSFNDFVIIKPPTPGYHHPSP"
     gene            complement(26860..>27573)
                     /locus_tag="PLO58_03415"
     CDS             complement(26860..>27573)
                     /locus_tag="PLO58_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73871.1"
                     /translation="ASLDWKFSDLIGFSLTGYYQASDNMPVTVSYPYGYEINPDTITA
                     AAWERFENRGWSRSNGIEFTMRTQRIHNFQYRLNVTYRFNKNGKTGLVYDANPENWED
                     IWYKPESYWSEKIIIDYQINYISNRLGIWVTLDAQQVPLEHFKRIYRSNSTYKTENGV
                     TYLWYQGMTYYYERYLRDTGGRWLFNFRVTKSLSQSMEISLFVNNILDDRAVWKNFDG
                     SLNEHNPPIFYGLEVSAQW"
BASE COUNT         7816 a         5852 c         5310 g         8295 t
ORIGIN      
        1 aaaatgttct atttggtcaa gctaaagttt tttatcctgc ttaatttata aatccataaa
       61 aagctgttga taaaatccag ttttgttatt ttatagatcg gaatatgaaa atatcggtga
      121 aattgcgaag tgagttggga aatatgatca agaagcggtg ttaaaaactt tgagcatcca
      181 gagaaaatag aagatgacac aaatcaattg gtttgtttca gttattaaat aacacatcat
      241 aacttaatac attacagtgt attaaataaa tatctttatt cttatgaatt attcagggta
      301 gaaagtaaat ctttcattgc caagttttct ctttatgcca atactacaat cagcattaaa
      361 atcattttcg aattatatta ggaaaccttc agaatgttta tttatggtcc ttatctattt
      421 atagatctcc gcctcaactt tctaaaggtg gaaagggcta caactttact cctcaatttt
      481 ttccgtgctt gattactctg ccctgtacta taaaaattat atcctcacag atattggtcg
      541 aaaggtcggc ggcgcgttcc agtttgcggc tgatgtccat cacccgtaag gcaatcggca
      601 cagttgccgg ttgtttgctc attaattgag tcatctcggc aattatttta tcccgcagat
      661 cgtcaaccac atcatctcgt ttgcagattt cttgggctga ttcggcatca ttattgataa
      721 aggcatcaat gctatcatga agcatcccga gcgattcctg cgccaggtga tttagaagcg
      781 ggatcggctt aaagctcggt ctatcaatca ggtaaagagc atcacgacaa atatgcaccg
      841 ccccatcacc aagccgttca aggtcattac tcatttttaa aatcataata attgtgcgca
      901 ggtcacgcgc tcgcggctga tactgagcaa tggtctgaat acaagcttca tccattcgca
      961 aatcgaactc atttgctttt gattcatcaa cattaatgat ttctttcagc gtattaacgt
     1021 ccttagcact cagcgcttgc agacttcttg tgatcatact ctcgactaat gccgcatatt
     1081 cgactatggc ttgttttaat tcttgaattt tcttcgttaa catattttac ctcatcatat
     1141 tttctattaa ccaaacttgc cggaaatgta atcttcggtg tgtttatttt ccggtatggt
     1201 aaaaatcttc tcagtaggcc caaattcgat caattcgccc aaatacataa acgccgtata
     1261 atcggagacg cgcgccgcct gttgcatatt atgcgtgaca attacaatgg tgtaacgctg
     1321 tttcaactgt tcgatcaatt cctcgatacg cttagtcgaa atcggatcga gactggcgca
     1381 gggttcatcg aacagaataa cctcaggctc cactgccaga cagcgggcaa tgcacagacg
     1441 ctgttgctga ccgccagaaa gccgcaaagc gctttcattc aggcgatctt tgacttcgtc
     1501 ccagagtgcg gcttcctcta gtgaacggcg aatggtattt tcaattgagt cggtactttt
     1561 aatgccatta atcttcaatc cgtagctgag gttgtcgcga atgcttttcg ggaagggatt
     1621 tggtttttga aaaaccatac cgactttacg gcgcaaagtt acaacatcca cacccgggtc
     1681 gtaaatatta tgattatcga gtagaatccg accttctacc cgggtattgg gaatcagatc
     1741 attcatccga ttcagcagac ggatgaacgt tgatttgccg caaccggatg gcccgataat
     1801 cgccgttacc cgattttgga aaatcgacat attgacattt ttcaaagtct gtttatcacc
     1861 gtagtaaaaa ttaacatttt ccgcctgaat acggatttcc ttagacaagt tcatatttcc
     1921 tctgtttctg ccgtaaataa atcgccagcc ccgaaactat taacactaga aataaaagta
     1981 tcaaagcaca accataggca atcatcgtct ggcgctctgg aaaagtacct tcggtcatca
     2041 gggcataaat atgataaggc aaagccataa cttcatccat aattgaattg ggaaaaccac
     2101 gggtgtaaaa ggttaccgca gtgaacataa tcggcgccgt ctcgcccgcc gctcgaccga
     2161 cgcttaggat aactccggtt aatattcccg gcattgccga cggtagcaca attttgcgaa
     2221 ttgtctgcca ctgcgtcgcg cctaatgcca gtgatgcctc tctaagagat tgcggcacgg
     2281 cgattagcgc ttcctgagcg gcggaaataa ttcccggtaa tatcataatg cccagcgtca
     2341 agctaccgga taaaattgaa actccaaatc cgaagaactt tacgaagacc gtcaatccga
     2401 aaagtccaaa aactaccgac ggcacccccg ctaagttatt gatgctcatc cgaataacgt
     2461 tgatcacaac gcttttcgga ctgtattctg tcaaataaat ggcgcatgcc aaacccatcg
     2521 gcaaagcaaa tattatcgcg ccgattgtga gataaaaggt gccgactaaa gccggtgcaa
     2581 tgccaccctt ggtcatcgcc cgtcgtggca cttgcgtgag aaattcccaa ttaatgacct
     2641 taataccatt gaccgtaata aaataaatga tcgcgccgag ggtaagtact gcgataaaaa
     2701 tcgctaaata taaaaatcca aaaccgaaat actgtatgcg tgaatttctt ttcatcgtcc
     2761 cagccccagt ttcaagcgat aattacggct aataatttcc gcaatgatat tgaaagccat
     2821 tgtaatcaga aataatacta agccgatggc gaatagtgcg tgataatggg gactgccgac
     2881 tacggcttcg cccatctcag ccgcaattgt agccgtcatc ggtcgcaccg gatcgagata
     2941 acttttcgga attaccgccg ctccgcccgc aaccattaaa accgtcatcg tttcgccgac
     3001 tactcgactc atcccgagta taattgccgt tgaaattcct gaaccggcgg caggaattat
     3061 cacgcgcgtc agagtttgcc agcgattagc acccaacgca taagaggctt cccggaaact
     3121 tttcggcaca aaattcaagg cgtcttccgc caaactacaa accgtgggca ccgccattac
     3181 tccgagaatc aagctggcag tcaaggcgca ttgtccaatc ggaatatcga gtaggttttg
     3241 cagaaagggc gcaatgatta ccatcccgaa aaagccatag acaatcgaag gaatcccagc
     3301 caaaatctcg ataatcggct tcaggatcga acgcaggcga aatccagcaa tctcatgcag
     3361 aaaaagggca ctgccgattc ccactggaat gcaaaatagc atcgctccca gcgtaatcca
     3421 taaagtacct aaaattaatg gcagaatgcc aaattcagcc ggttccgccg tcggatacca
     3481 cgccttacca ccaataaatt caaaaaggtt gacaatacga aaaatcggca aaccctgttg
     3541 aaatagaatc aaaataattc ccagtaaaaa caccaacgaa gcaaatgcca atccaccaaa
     3601 tactggtttt actacctttt cgctattcca tttcatattt agttctcata atgacggcat
     3661 ggcagacatt tgcctgccat accataaatt aatactatac aaaaaaactt cccgatacta
     3721 ttttaatcca acatatccgg ctgtttcgac taactcttgc ccttcctcac tgaggataaa
     3781 gtcaataaaa tcctttacga cgcccttggg cttgccattg gtgtacataa agagtggccg
     3841 cgaaagcggg taagtcttag tcaaaatgtt tttcttggtg caggttacat tctcaacggt
     3901 aacagccttg actttgcttg atatataacc gataccgacg tagccaatcg cacccggcgt
     3961 cttttcaacc gtggttgcaa tggcttgatt agaggcattc atcagcgcat ctttacggac
     4021 tttttcgtca ttgagagcta atttactaaa agcttcgaaa gtgccgctgg cggtatcacg
     4081 ggatagcacc acgattttct tatcttcccc gccaacttct ttccagttcg agatttttcc
     4141 agtataaatt ttccgaattt gctcgacggt cagattttta attttattcg agggatgcaa
     4201 aataactgca atgccatcca tagctacgat atgtgccact ggattgacgc ctttggcaac
     4261 ggcattttta atctcctcat ctttcatagc gcgcgaagaa gtggcgatgt cacaggtttt
     4321 atcaattagc gaggcaatgc caactccgga gccgcctccc tgtaccgaga tcgtcacgtc
     4381 cggattagca atcataaact cctcagcggc taactgcgcg atcggcaaaa ccgtggtact
     4441 accttccata cgtattttga cagcttcacc agccgttaac agcgacgcaa ttgccagaat
     4501 caaaattgat aggttaaata atttcttcat ttttagttcc tttctttgtt agaatttaaa
     4561 ttgaatgtca gtttggagaa cggtttcagg tttaatcgct ccgtgtaggt tctctatttt
     4621 atagtaatcg agcgccagga cgatattttt attcaaaccg tactgggcta tcagttcgct
     4681 ccccttaatg tcggtcttac caccataagt atcggaatcc ggtagaaaat ctaaccaagc
     4741 gtccttttca agctggcgat aaacatattt cacttgccac tggcctttgt ctttgatctt
     4801 ttcggaaccc aaagttaaac cacaagctat gccattttta gcagtgtccg attccgcctg
     4861 gaggttttga ttaatttcaa aacagacacc gatataggga aagattttgt tgtatagggc
     4921 aacttcggta gcaaaattga agttattgta gcccaacgcc agatatttag aatcattaat
     4981 ggtaatataa gaattagacc atttggcatg atcgaaagcc atattctttg ttttattgaa
     5041 attataatag gtcgcggcgg cttttaaact gtatatacct tctttagcca gattgaaacc
     5101 gggttgtaaa atcagcatac gcggattatc ggcaatgaca ttttctacct tgcccttttc
     5161 atccagaaag taaattccag tattaaagaa tatcatttta tgattgaatt gcgccgcaac
     5221 gccttcgacg gtaatgtcgc tatcccacat ataatcggtc ggcgtccaga agccgagatt
     5281 gctgtaaaat ttgccgagca gaacgttcgc atatttcggg aaggtataac tgccataagc
     5341 atagtccagc ataatcggct tggtctcgaa agaattttcc agcgtttgat tggtagaacg
     5401 cggatcactg gaaccagttg ccaatccgaa gccaactttg aatcccgacg tcactacggc
     5461 ttcaccattc aagcggaagc gaatccgctg gcgcatccgc gaaacggaat cttcttcggt
     5521 ggcttcccac tggttgcgga atcggaaatc gccttttaat cccatattct gaatccattt
     5581 cggtaaagtg gcagattttc cggcattcat ttccgctcgc atttcttctt cggtcaaagt
     5641 caaaatctcc tgagctttac cggcatccaa cacgcccttc tcgactaatt cattaactaa
     5701 tcggtcaagt tcaccggcgt tcagcgttag ggcacaaaag ccgagcaacg ctaaaataat
     5761 gttgattttc ctttctctca acttcatttt tactcctttt tgttggttga ttcatttttc
     5821 acgccgataa ttaaggaatt cattttaaga cttggtaaac gaacggttaa tttttggtta
     5881 agaattatta tttacaacgc tggcacagtg aaaagaaagg aaaaaggctg agaatggcca
     5941 ttagtcattg gtcattggtc attagtaaag aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nngtcattgg tcattagtaa agaaagttga ggcagagaaa agaattttaa
     6121 tcttagcttt tcttttaggt taagactgat aaaagtcatt acttagccaa aagaaagctc
     6181 ccacgaggcg gactatttgt aatcaaagag aaagggaaac tgattttcgc ttctgaattg
     6241 attttttagc ctttattgac ttcccagatt tcgccaccga agtaatccca gggcagacgc
     6301 cattcgtcac actcggacgg ttcgggagaa aattgcttat tcataaaata gattaggtgt
     6361 gcttttttga gtcccagatt tttacagccg tgagccacgc ccggcggaat aacgattaat
     6421 tgggaattgt cgtcgccgat gaccaaacgc attctttgtc cattagtcgc cgaatcctcc
     6481 cgcaaatcaa ttaaaatgag cagaatctta ctgtccggcg gaacaaacca gatgtcggtt
     6541 tgctgacggt ggacgtgaaa agctttcaca ctctgcggtt ccatctcgct gaaattgatc
     6601 tgcgcaattt taaaattctc gactccgcta aaatcaccat tggttatgcg cgccagttcg
     6661 gtaaacgaac cgccatcgtc attatggcgc cgcagagtaa tcaccttaac tccctcgatg
     6721 gttggctttc cagcataatc ctgcaagtgc acgatttgtt caatttcttt ttttaactgt
     6781 actttcaatt tatgcaaccc cttttcttca aaaattagat aactggtaga aattatcaca
     6841 ttgttactta aaaaaccaga taatccgctt caggcgcata ggaatcaaga ctttgctcaa
     6901 tatgcttatc tgctatctaa ttgataatcc gaatctgtat ccctacaggg aataaatttc
     6961 cgacaaatct tttaccttgg agcgattaag ctttctaata aattagtgaa agtcttgtat
     7021 aggattgctt gacattcgcc tattctgctg gtaaattttc gacagttttt ctaaatatat
     7081 ggcgatcaca gttttcataa tatctctatt tatcggcata ttcgtactca atttgctgat
     7141 ttttaaatat ttgaaaggtt cagcacctca accattcttt tcagttacct atcgccccaa
     7201 gccgctagcg gcttttgatc gctatacttt gaaagttgtg gcgctcacta cattgataat
     7261 tatcattttt atgttaattg ccatagcact ccaatctgga tttgttatac cggtcatcca
     7321 tcctatcgcg ctaattttat tcattttcat aacgggggtt ggctttgtat attctttcag
     7381 aagtgaaggt aatagatcca aatctgtcca gaaattttct accggagaga ccgacggtga
     7441 ggagcaaaag tgaaaagcga aaatgtcggg aattggcaaa ccttacgttt tatatttccg
     7501 gataacgccg ccatatcctt tgacgcccgc attgcggatt cagtaatcaa agaactagcg
     7561 ctggatactg gtcaaccggt tttcaaattg agatgttcaa taaatgaact ttatcagccg
     7621 aatacccaaa tttttaccag cggtttccct tataatgact tgtggtttcg ctatgctcaa
     7681 atattagtca tcgaaaagca tcttaaactt caggtgccgg ttggtattga attaatccgc
     7741 gtaattttct gcgagtttct tagaattgcc gaatatattt ttaatttagc tacactttgc
     7801 ttaaatgggg gactaaacga cattttttcc ggtcttatgc aacagtacga atccattcag
     7861 caatatttcg agcaaatatt aagtggaact aatccgatgg attttattat tggacataaa
     7921 acaccagaag acctgacggt cggatttacc gaaatgactt tgaatctctc gcaccagctc
     7981 cagattcaac tccggaaatc tttgcctcat ttactcaaca accgcctgat tctttcccga
     8041 tttagcaaaa tcgccgacca gaccgctgat aattcagcca ttttaaatga taannnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatttag caaaatcgcc gaccagaccg
     8221 ctgataattc agccatttta aatgataaat tagaattcga tcaaatgcga tttacttcca
     8281 gtaaaaaaca aaataatgtt tgctggcaac ttttgaaaat acgttgtgac gacctatttc
     8341 gatcaatcgg tttgattcgg ctgtctctaa cgaaatttgt ttatcttgat cggtcgccgc
     8401 aaacactgcc ttcgctaact tctcttccca aagggaattc gagtgtaaat atcgaaactc
     8461 cttggggcaa attgtcctgc cagatgaatt gtccgggtga cagaaatccg tgtgatttta
     8521 tactatcaaa tccgcttcac aaaatccaac ttctgttatg caaaatggct gtcgggcttg
     8581 agttggacga attaccgctt ctgattgcgt cactcgatct ccgaccgaaa atagatccac
     8641 gttgatttaa atgccaatgg ttatatgaat attctattta aaaatttgat atatgggtta
     8701 ttctgggtag tgattttcgt agtcataatc ccggcattat caattattga acaacggtat
     8761 tttaccggcc attttcgatt aacggaaaaa aaagcgaaac cgattccaag ttatttgcaa
     8821 tattggagca aattttggtc aagtctccga ggtgaaaaat tacttttgtc tatgactact
     8881 caagtttttt tatggatcga aaccggttat ttaatggctc tttggttagt tttgccctat
     8941 caaatgcaaa ttcagggtaa tcagacgtta tggttattcc tggcaattat caccttaccg
     9001 ctgaatatta tcaattcaat tatcgctagt gaagaggtaa aatccgaaat cggccaggct
     9061 ggaattttca aaaacggaat tagcctttta agcggtttcg ccccgttgct tctgtcaatt
     9121 ttaattccta tcacgattaa tcgttacacc agtttttggg aaatatcaag ccaacagagt
     9181 gggtaccgct tgggttttat tccaaattgg actattttta gttcgccatt attcttgctt
     9241 attggaatca tttttctgat aaatttgacg atgctaatgc gccttagcga cggcagttat
     9301 aaaccgttgg aatatgaaga accggcagag atttacagtg ggcggcacga atctttcggc
     9361 atattgccgc aatttaaccg ttcggcgatg ctttttaccc tgactacttt ggcgattttt
     9421 ctatttttcg gcggttggca aacgcctttt gagcctgagg gacaaaattt agcttcgttg
     9481 gccggtggac ttattttctt cgttaaaacc attctggttt tcagcgcttg tctggcgatt
     9541 aagtaccatc tgccaattct cactaactgg caaattatta agattaacct tagaattcaa
     9601 attcctgttc tgactttgat ctggctgggt ttgttgatca ttaggtggct ctgatgacaa
     9661 attttcaatg gatgcctgtc attttttggt tggcgacttt tttgaccttg ctcccagcgg
     9721 gcttggcaat tacctcgacg aatccgaaaa acctcatcct gttgaatatg ttcactcaat
     9781 tgggtatttt cctactgata ataagtctgg gagtcggctt cccggctgtt atcttcctcg
     9841 ttctggtttt cccattaaat ataatgatgc tttatggaca taatttattc cccgaaacaa
     9901 ataaacaaac cgccagagaa tcacgcccgg cgattatttt gaaaaccgcc gtatgctttt
     9961 tgcttttttt acttttaatt atagcatttt atcaaaacca tctgaaaaat atgttggtca
    10021 tcgataattc aggaaagatt ctggaaggcg ttatccctga cgtttctttg ttattgattt
    10081 tgatagggat gtttataatg ttaatcctaa tcgctatcag ctatttttta aggagaaaat
    10141 gagccacctt tttttatatc aaattgtcgg tattggactt ttcataatcg gtctatatgg
    10201 cttgtggcgc aatcgtcatt gctgggcagt aattactttt ttgcaattaa tgtcaagcgg
    10261 catcttactt agttttttag caattactcg gacaaaaggc gcttatcaat ccgtgtcaga
    10321 attgattgta atatttattt ttgcggttct gattattgaa atacttttgg caattgtttt
    10381 tacttataaa gttcttatgt ccgactcatc ttcaaattcc gacgacaaat ccaatcgagg
    10441 ttaaaatgaa tattttgatc attctacttt tattacttcc gatttgcgcc tatttgctta
    10501 ctcgatgtct gccggataaa tgtttattga atcggcgcgg gctgtcaatc ctatttttac
    10561 tgatttctgg attgatcggg cttggaatat ttatcgttct ccttctgata aacatgcccg
    10621 attacgttca aaggatttct tggacttggg caaatattgc cggtcactcg ctccagattt
    10681 cattattgct gaataatttt ttaggtttgc tattagcgct ttgggcgctt ggcagtttaa
    10741 tttttgcgat tttgactgcc accgattttt ttagccccga tcattttcgt gaaaatatta
    10801 ccaatcttgc gctgggaact ttggcggtca acggtttgat tttagccaat gactttttat
    10861 cgttaatctt cttctgggcg ttaattgatt taattattta tcgagttgtt ttcagaaaaa
    10921 ttaataaagc gtccgaatca tattttccag acattatggt aatcggtggt catttattac
    10981 tgattctcgt caccttttca ctttttaaaa tcagcggcac gtttcgattt gatctgatta
    11041 tcgaagaagt attatcggga aaagttgacc cacagcaact aatattgcca ggcagtgtgc
    11101 tcattctggt tggaatcctg cgcatttatt cgcctttctt tcgttatcag tttaatagtc
    11161 agacgaaaat gtcgattagt gatgaattca tccttttttg tggcttgatg ccgtcgggtt
    11221 tgttcctggt gttaaaatta ttcccgatta tgccccagat atttttaact ggcttgatga
    11281 tattcagcgg gattagcgtg ttaatttttc ttataattca tttagccaag accggattta
    11341 atcaactttt acatctcaat acgatatttc taatcagttt gctcatcatt acaatcggtt
    11401 tgggaacttt tccgataagc gcttccctcc tttttattct ttttatgacc aattttatgt
    11461 taattatttt ggcacaagat ttccaaccga tgtctgattc agccaatttt tcaattgctt
    11521 tgccctttcg tctgattgcc tggttaagtc tgattgggtt gccgatttca ttggctttca
    11581 acaccagact tttgctttta caggcttcat taacattatc atcaaacaag ccgattcacg
    11641 gaacgattat aagcgcctta ttggttttga attatttcct aaatgttgtg gtagtgttca
    11701 gaatttttct aaagtcaggc tggtatgaac cgaaacatcg taaaattgat cgggttacgc
    11761 cgctcaccca ttgcataatc gccggtatta gtattgtttg cctttatcta ttttttacct
    11821 tgcctaattt caatccactg acaactgaac tgaggttttt tccactattc tcgatccagg
    11881 gcagtcaacc gctcgattac agccagttta gttcaaactc actaatcatt tactctgcca
    11941 ttctgttaat tggcttagtt gtcggattta taacggcaaa atatttcaaa gccgagaaat
    12001 tctctcgctg gctaactgaa tttagcctcg atttccgaag gtatagtatg ataccggtag
    12061 cgaatttcag tcaggtagtt gcgcgaaata ttaggcattt cgatacggtt ttattctcaa
    12121 gagtcagcca aagtttaatg caaacagccc aaacggcaat cctatttatt aatcgaatta
    12181 atgcaatcgg cgctcaacaa ataaccaaat tgaaagaagt tcctgaaaag tatttaaata
    12241 gaattttaga tgggatcttt aacctgaaat tccaggtttt aatcggaatc gttttaagta
    12301 tattaataat taccgtttta atctgtattc tttaagtttt tttccggagg aagtgattta
    12361 tgactgaaca tctattatct atcaatttat tgattgcgct gatcggcttg ctaggaattg
    12421 cactgacaaa acgcgagaaa ggcaatctaa ttaaaactat tgccgccatc gttaccggtc
    12481 tgcaattctg gaccataata gaaattttat tcaattttaa tccgatggat accgccctgc
    12541 agttttccga acggattaat tggattccag ccttccgaat tgactattat ctcggcgttg
    12601 acggcattaa tctggttttt ctaattgtta cttccctatt gattttcttg agtgtgataa
    12661 tttcctggaa taaaagagag cccctaaaag agtattttat tctgttaatg attttggaca
    12721 ttggtttaaa cggaatattt atagcgataa acttgtttct atttttaatt tttcttgggg
    12781 ttactttttt tgctatctat ttgataatag ggtttagtaa tatacgagga agcgttaatt
    12841 cggttaaccg gttcggaatt ccctttctga taagttatat tttcattttg ctcggtgtac
    12901 tactccttta caagagcaat aatctccata ctcttaacct gaccgaatta actgtcggca
    12961 acgcgatttc tcacagcaaa cagatggtga taagtataat tttattaatt ggtttcgcca
    13021 tcttgattcc actttttcca ttccattctt ggctgattcc attaattaaa aatgggccta
    13081 aggaagttag cctattgatc ttaggaatag tgaccaaagt cggcttgtat ggattggtcc
    13141 gtattatcgc gccggtaatg ccattcgcta ccaactcatt gaccctggcg cttggtatct
    13201 ggggattggt aaatataatt tacggagcaa tttgtgccct cgggagcgat gatagcaatc
    13261 agattcttgg ttattttact cttcaacaat taggattctt tttaatcggt tttgctacgc
    13321 tcggcgaaaa ttcaaacatt tcgatagcag tgacgggttt gagtggtagt ttgctaatcg
    13381 ttatcagtca ttcctgtctg gcgactttgc tattaatcat tttcaaaaat attcgagcgg
    13441 aaaaccagaa cgttgctcgg ccatcactcc caaatattgc gatagtaacg ctcacgctat
    13501 tggctggcat tggaatgcca ggttttttag atttttttgc tcgcttttta attctattga
    13561 gtgcttttca gactcagaga ttgttgatat tggcgattat tagtttgctc ggcatatttt
    13621 taaacttcgc catttatctg aaactgttca aaaaactggt ttttagccag caaactcgcg
    13681 atagcgcaaa aaggttagat tcgaatgagg tatttgcgtc ggtcagtttg ctgatcgttc
    13741 taatattgtg cggcatattt cccagttttc tactaaacat catccaacct ggaattactc
    13801 gcctgctgga aatgttcagc acggcaatcg cattatagtc taaaaatgtc aaattttcag
    13861 tctatcatct atttcatacc ggaaattatt cttattattg cggcgattgg atttttattg
    13921 ctgactactt tttttaaaat atctgattct aaaaaatatt ttctgatcag tgttagtcta
    13981 ataatttcat taattgccac ccttttatcg cattcagctg acggacatag tattttcaat
    14041 aatctgctgg tgtgggattc atacggacaa ttcatcaaaa tttttacgct tataatagcc
    14101 tgcgttattc tcataatccg ccgtaaggag aattgtaatg aagccagccc gtgcccggtc
    14161 accgagtttg gaattggact tttgctccta attagtgtgg cgtttgtgag cattaatgcc
    14221 aatcatctcg tcgctttgtt catatcactt caaattctgc acctcgccgg tttttttatt
    14281 gttcagagtc ggaatctcag tccccggatt aataccgcca gttggcacga tttaatgttt
    14341 gacatcttca ccagtttgat aatgttaatg ggaatagcgc taatttatga taccacaggt
    14401 acttttttca ttgctgaaat cggccaaatt ctcagcactg tgactcgtat gaatttccct
    14461 ttgatattag gaatagccct gttattagtt ggattttcct ttactattac tggaatttat
    14521 cgcttgagct ggaaattaga gatattgcgg acgatttccg cttctgatag gctattgctt
    14581 ttaacgattc cgcaattcat cgaaaccact accttgatac gattactcag agtcgggctc
    14641 ggattggtcg gtgattggcg tcctctaact ttaatgatag gaattttcgc cgttctaacc
    14701 cttttgtttc cgattatttg gctaatcaaa ttacgttcgg cgaaggatat aattatagtt
    14761 acttcaatag ctcaaagcgg tttcggactg ttagccttat gcgccggtaa tatatcggga
    14821 gatgtagccg cattgtttta cgaggttgtc ttgattttca gtttgatagc attcacgatc
    14881 ggagaaattt taacgatcaa tgcaaaatcc agccagttaa tcgcattagc gagcctaatc
    14941 ggtattccat taaccgccgg ttttccagcc aaatatctgg tttttattag tctcgttcag
    15001 cacagtccat tctactattt tttaattgta ctcgggatcg gtagtgccgc attgatgtta
    15061 ataggttatg tacgtatagt aaggcaaatt atcaccgcac aagggcaatt tttcaattca
    15121 ccgcaaaaag gcagtcagct gatatttttc atccagattc tgattaacat tatattaatt
    15181 tcaatcggtt tttactggac gcctctgacg gattacctcc aatccataat gactttctgc
    15241 gttccttaat ttcgtttgac caacattatt ttactgatta tttctattga tcgtattata
    15301 ttaaaattga tgtattacta gttaaataag gattactatg tcaccacaaa ataaacgctt
    15361 ttttttctgg ttcgttattg cgtttctaat cattttcata attttaatat caatttcttt
    15421 aatccataat ttcaataaat ccaagataat cagtttacca gcgcaggaga tgcggggcgt
    15481 ctggatgagt cgcttcgatt acactgaacc gtttccatca tacaatcccg attcgatgaa
    15541 aacttatatc agtgattcct ttcgccggtt aaaagatgcc aatttcaatg taattttttt
    15601 ccaggtacgc ggcaacgccg atgcttttta cgcttcacgt tacgaaccct ggtcgaatct
    15661 tttgaccggt aaattaggac agaatcccgg ttgggatccg ctggcttatg caattgaaac
    15721 cgctcatcag ctgggactcg aattgcacgc ttggcttaat gtttttccag cttggcgggg
    15781 aaccgctccc ccgactactt ctcaaccgct ccatccttat ttggctcatc ctgactggct
    15841 gatatgtgat ggaaatggta aaaaaatgct tctctcagat ggctatgtca gtttttctcc
    15901 gggaatcccg gccgttcata atcatttaat aaaagtaatt tcggatatcg tcagtcgcta
    15961 tgatattgac ggaattcact tcgattacct acgctatccg gaacaatcgg aactcaaagg
    16021 atattcacac gatccaatta gttgggctcg tttccaatca catgaaggca atccacttgg
    16081 tctggactgg acagattggc aaagggaaca gattaacgaa tttgtagccc gcgcctataa
    16141 ctgcgtaacc gacattaaac cggagataaa agtctctgcg gctgttatcg gtagctacaa
    16201 gaccgatcat tggaatggtt attcagcggg atttcaggac ccacggcgtt ggcttgaaat
    16261 tgggaaaatt gatttaataa ttccgatgac gtattatcac cttaatcaca aaaattttac
    16321 ttttaataag gctctcaaag aatggcaatc agtgattgat aacccggatc aaatcttccc
    16381 cgccttagcc gcatacaatc tcagttggga agaaataatt gaggaaatcc atttagttcg
    16441 taatagccat ttcaagggca tggttttttt cgccgctagt tcgctcgata gcgaaaagct
    16501 ccaatcactg caaacaactg aattccaata tccgtctatc ttcccatgtt tgacctggaa
    16561 agattcggtc gcaccgccgt ccccaattaa tttcgcagtg gaaaaattaa catcagattc
    16621 attagaattt atctggcaaa tccctcatca atccaatgat attaaaaaat ttgtaatcta
    16681 ccagtcaaaa aacctgccaa ttgatttaac aaagggtgaa aatattttgg ctatcgtagc
    16741 gggtagcgat acttcctacc aaacgaagtt caatctacca gatcagcgaa ggtattatta
    16801 caccataacc agtctcgacg ccgcttataa ccaaagcgaa ccggcttcgg tcattaaaat
    16861 caaataacgg attttatcag tcggagcatt tttatcacat ttccagataa aatatgtgac
    16921 ccagatcata gattgacaat ggcttcgact ctggtaaatc agatttttta ttgcaatagg
    16981 acatatattc ctctaaattt tacgctaaga acataaaata tgaattacta ttataacata
    17041 cgactgggaa tattttatag cctctatgct gaaaggattc ttgatgaaag ttaaattctg
    17101 gggcgtacgc ggctcgattc caactccccc aacttccgaa caaatccgcc ataaattttt
    17161 aagaataatt gacaatttac caaccaacat tcggaaaaat ccaactgaaa ttaagaaata
    17221 tattctcagc ttgagctatc tagaagccgg gctaattggc ggaaatacct cctgtgtcga
    17281 aatacgagcc gataacaaaa tattaatatt tgatatggga agtgggctga gagcactggg
    17341 caattattta gtaaaaaatg aacaaaataa aaatggtttg gatttacaca tttttatttc
    17401 tcacactcat tgggatcata taatgggctt tccctatttc acgcccgctt ttttccccaa
    17461 taataaaatt accttttact cgccacatcc cgatataaaa gaacgcctcg aaatccaaca
    17521 agattcccgt ttcttcccgg tctcgctcga acatatgctg gcgaaaaaag aattcattca
    17581 attgcaaccc aagacttcta ttacattagg aaaagtaaaa atttccaact acccgttata
    17641 tcacccgggc ggctcgtatg gctaccggat tgattgggat ggaaaaagcg ttgtctatgc
    17701 cactgattca gaatataaaa acctcgaccg tgaatctacc aaacactata ttgatttttt
    17761 tcgcggggca aatttattaa tcttcgatgc tcaatatact ttcgaagaag ccatccataa
    17821 agaggattgg ggacacagct ccgcccttat tgcagttgaa tttgccgtcg aagccggggt
    17881 aaaaaaattg gctctattcc atcatgaacc ggaaagagat gattttgaaa ttaatgatat
    17941 tctcaaaaaa tcacgcgatt tcaagaaaat aaatttccca aacgaaatac tggacatttt
    18001 cctggctatg gaagaattgg aaattactct ataactggaa tcctattatt ttaattttta
    18061 taaaatctta gagttatctt aacctgctaa tttataaatc cgtataaagc tgttgataca
    18121 gtccggtttt gttattttat agatcggaat atgaaaatat tggtgaaatt gcgaagtaag
    18181 ttggaaaata tgatcaagaa tcagcgttaa aaactttgag catccggaga aaatagaaga
    18241 tgacacaatt caattggttt gttttagtta ttaggtagaa tattaaaatc caacaatcca
    18301 cctcattatt tttcaagcgc gtacatttcc ggtgttactt gataaattct tagcggttca
    18361 cggaattcaa aggaattctc catctccatt attttttcat cagaagtgag tattttcagc
    18421 atgggttcat taagtttggt ttgaatgtct ttcggattta cctgggtagg gtcgaagtat
    18481 atcaaaagcg cttcgcgatc agtgtagtca gttcggactc taacaacccc gtcgaaaaaa
    18541 gaaatatgac tggttaaata ttttaatgcc cgccgcacgg caatgctctc ggcatcaggc
    18601 aaaccaatct caaaaatcat taaatcagcg ggtcggtact tctcgtaact attgaatgcc
    18661 tgatcatatc cagcgaaaag ccgacgccgg aaagtaacat aatcaagggt atcaacaaaa
    18721 gcgccccgcc cttcacaacg gaaattgacc ttcacgacct cggtttttcc gttaggcaat
    18781 tttttctcgt aagatttttg gttgatcaaa taaataattg agtcgggagt tactttgcca
    18841 agttcgacat atatcctcgc tatgactggt tcgccgaaat tagtctcgaa accgacgatt
    18901 ttaggattga cgcgcaacat acggattagg tcggcattat cctgaacatc ccataatcca
    18961 ctaattggaa tttcaaaaac ggcgattttt tcaggctgat attcgtcgaa tttctggatt
    19021 ttgtactgat aaggcgtaaa aatggctttt ttgataccat attcgtcaat tttggtgctg
    19081 tcatagtaca caactacttt atgacttttt acgtaagtat ccaagccaac aattcccggt
    19141 tgcttcatta atttattttg aagtgatttg gaactgcccc aacatttaac ggattttaaa
    19201 ccttccattt caaatttctg aactgattcg agctcggcat aatttcccca ccgctccgag
    19261 agtgtcttaa actcaaagtt acgggctgtt aataaagcaa ggataaaaag taccactaaa
    19321 gctactggag gaagccagcc ataatttcct ttgttaagcc taatacttcc actactggag
    19381 cattctttgg tgcaatctaa acaaagtgta caatccggat gagtgacttt ctcgtaacta
    19441 ctgaccggaa taccttgcgg gcagttatcg tcgcagttac cacaagtggt gcaggtattt
    19501 ttattaatat taatccggaa aggcgaaatt gagaaaaatt taaaacgtag gatttccgtt
    19561 aatgcgccgg acaggcacaa cgccaataat aaccacagga tatgcaattt gataccaaaa
    19621 atatatagac caaaataaat aatcagaatg ggagctgtta tcagaacatt actaaagata
    19681 ttgctaagag ctcccagagg gcaaatatat ttgcaccaga aaaatcgaat aaaaacggat
    19741 agaactacaa ctgtaaaaat agtcaaaata ccaaccaaaa gtaccgtatc actgccaaac
    19801 ccagtaactg cggcataata gggatcgaat tttttgcacc acagttcgct ggcggtagcg
    19861 gtaaaatagg ctgtgaaaaa gagcaaaacg tatttaccga ggcgcagaat ccgatccgct
    19921 ttccctttca gaacaataga aaatttaaat cgggcaaaaa gtttatttaa ccactcggtt
    19981 actgtgccaa tcgggcaaat ataaccacaa aataatttgc cgattagaat aacgccaatc
    20041 agcagaaaaa gtcccataaa tacctgggtc tcattcatac tgcaggacat cgaaccgaga
    20101 tgaaatttac tcccgagcga aactaagcca ccgaagggac aataagcctc gaaatcggct
    20161 tttttagctt tatcagtgca tccgatgatc actaaaataa tgattaataa caggattata
    20221 gcctgaaaca acagtttggt tttattatgg aaaaagttcc tgcgcatcta aatgaattcc
    20281 ttcgttaatt ttctggaaat agtctggcaa aagtaagtag cccgcactct ggtaagaaag
    20341 gactgtcgag acagcccttt cttatcataa ttgcccaatt agtttatttc acaatagtca
    20401 tcttcttcac atttctgtaa tctccagcct gtatccggta aatatacgta ccacttggca
    20461 agttgctggc atcaaagttt acgtgataat aaccagcttt cagaaaccca ttttgtaaaa
    20521 cggcaaccgg ttgtcccagc atattgtaaa ccatcagcgt gacctgggtg ctctttggta
    20581 aggcaaaagc gatcaccgta gtcggattaa atgggttggg ataattctgc cgaaggctga
    20641 attctgttgg taagccggaa gaaccgttcg caacgccaac aaacgataga gcagtcgctc
    20701 cttcatccgc acccatatag gtgacattgg gtcgcgcatc gccatcaatg tcggttgaca
    20761 accaatcggg acgatgacag gctaaatcgt tgtcaccgat agaactgcca gtaacgtgta
    20821 aatcagttgc cgatacgaaa ttgaccggtt tattgattga attggcatcc tgaccggaag
    20881 cggctctcca tgtggcaaga tcggcacgtg gtatcgaacc atagaatccg accgaagcac
    20941 tggaaccagc aatataaata ttgttataat tggaagctaa tccactattg ggcgaaaccg
    21001 tattgtgcca atgaatgccg aatgaattgg cggcgtcgtg acggttggaa aggatattgt
    21061 tatagatatt taacttagcc gaaaagggaa tttccgttcc aattgcggcg tgaaccccgg
    21121 tttgattggt gggcgcaata tcaatcgtat tgtgatagat attagcttcg ccactccaat
    21181 ttgccgaacc aaatgctatt ccataaactc gattatcagt ggcagagccg ttattagtag
    21241 tattcatcgc gataaaatta ttgaaaatat ctatgacacc accgttttca ccaataacta
    21301 ttccaccagc gaattgaggc gcagttgtgt tcatttcaac attaactatg cgattattaa
    21361 aaaccacaca ggtgtcgcca gcggcaaatc cgtttaagta cataccgcta taaaatgttt
    21421 gatcggcaag cggattgata atagaaataa cattgttgat ataggcgttt tttgagatgt
    21481 agtcagccat tataccggta ccagcggcat agatatcaca gcccataacg acagcgccgc
    21541 agggagtttc aggcgaattg cccctaagcg ccactccgtt agcgacggtt ttttcaggag
    21601 tcccgatctg gcaattttta attaaaagac tattaggaac accggtatat ccatctacgc
    21661 ccaagattga accgcttgcc agcagatttt caaaagtgat attgacgttc ttgacggtga
    21721 tgaaatcgga attgtcgatc acgcctatta cacacttgcc atcagtatcg ttaccaataa
    21781 ttgtcaaatc gcgagaggtt ccaccgctgg tattactgcc atcaatagtg acatacgcgg
    21841 tattttcaat agtaaaacct tgcccggagt aattaccggt agttgggaaa tcattgattg
    21901 taatggaagg cgttttgccg gaagccggtt taatcgtcaa cgcagtttcg gctgtgagat
    21961 cttcgcgatc gatgattaat tcagagccgg gctcaatcaa gtcggcgtcg atataaaaag
    22021 taactggcgc tatggcacca taggtattca gtgccgtgac agccgcgccc agagtagcaa
    22081 aaccctgttc gtgatcgccc tgagggatgt aataatcacc cgccagaaca ttagagtaga
    22141 tgacgttttt caggatagtt ccattttgac caactgccca tagaataccc acactgccat
    22201 tatcactgta gcggactttt tgcagaacct catctgttgg actggtttct accgtccaat
    22261 tcagaccggc atcttcagac ttcaaaatag ttccgtattg tccgacaaca aatacggtat
    22321 tgtcatcaac aaattccaca tcatatagtt cagcgccatt actggcggta taaaccagag
    22381 taggcatagc atcccaggcg gttgagcggt aaatatagcc ggaggctcct ggaattatac
    22441 cgatgccgtt gcgaaattcg accgcccgca agtttttagc ttcactggtc gccccaagtt
    22501 tggtaaaggt catcaaatca gtcgaagtat aatattctcc actagcgcag gttgctacga
    22561 ccgcattgtt ggacaatggc cagatatcgt aaaccgtttt ggaaccagcg gtagctttgg
    22621 tccaagtgtc cccaccatcg aagctgtaac aaatatagcc gcttccaccc cctacaaaaa
    22681 ccgtatcgga atttaggtaa tgcatagcat taacggcttt attgttatta tacatcgggt
    22741 tatcaatctg agtaaaagta gcgccaccgt cagtggtttt caaaatgtat ccaccactgc
    22801 cacctataag tccgttatta gcgtcaataa attcacattc aaaaatatcg ccgtcgagac
    22861 cagtcctcag attgttcgga taagaccagg tttttcccca gtcgttgcta aaggtcattt
    22921 caccaccacc gtctgcagtt aatacgattt ttttctcgtc cgggaaagcc aatcccaacc
    22981 agctgattcc aggccaatta ttaataggaa tccaattcac cccaccatca gcagttttca
    23041 tccattgctg gtattcgcaa aaccagtaac cggtacgggc atcaataaaa tcagccgaga
    23101 gaagcctttg acctgttcca gtactaaaat tagtccagtc cgttccaccg tttgtcgtta
    23161 tagcaccaaa attatcagat ccaccaccat aaataatggt attattatcg aatacctcaa
    23221 cataagaggt atagacattg gcaaattgaa caacaccttt gtttgcaaat gtccgaccgc
    23281 catttgtaga taccagacag acccgatgat aacaggctag agctacggtt gaatcgccac
    23341 cgaccgcgac atcatagacc cgataagaag ttacaccagg cggaaaagct gcagctattt
    23401 tcaccggatg ccagtttgtg cccccatcct cagtccacca gatagtcgct tctttattaa
    23461 aatccacggc aactactccg cggttttcat cgaaaaaggc aataccacca tcaagatcat
    23521 cgcgggtttc atcactggcg gtcatatcgc catcgaaatt gtaggaaagt ttattgaaag
    23581 tagagccgtt agtagttttt agaaccgtac cgcttttacc agcgcaatag accacattgg
    23641 cattgaccac atcgagacta tacagatcga atttgtaggt attggtatcg ccgatcatag
    23701 tccaatccga gccgccattg gtggtcttgt agattatgcc accattgccg caggcataac
    23761 ccacattggc atcaataaac tccatatcat tccaagaatt ggcggataca tccggctcac
    23821 gcacagtcgt ccagttagca ccaccatcag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23941 nnnnnnnnnn gaattggcgg atacatccgg ctcacgcaca gtcgtccagt tagcaccacc
    24001 atcagtagtt ttttgcacct tgcccatttg tttgcttgga taggttgtac tatatactat
    24061 ccagccggta ttagcatcaa taaaagacgc cttgttaacg gacatatccg tcgaggcaat
    24121 ttttacggtc tgccaagaac cgcctaagag taggttcgca acagcaaata aaagaactgt
    24181 aacttttaaa gttgtcttca ttttttctct cctttttaaa ttatcatttt tttatgccca
    24241 cacttgaaaa tataagcaat aaataaaatt atatagatca taatttatta cttccaatag
    24301 gaaattttta gaccaatggt ctgattatat tgatccggat tgtcgaaggc agtttggaaa
    24361 tattccagtc cggctccaaa ccggccgaaa gttgttaggc gttcaattcc aatttcgcac
    24421 ccatagcgag ttgcccgctc agcattccaa taaaaataca aatcatcaat cgccagattt
    24481 cccagaaaat agactgacaa aatctgattg atggcatagt ttaaatcgag gaaggcagat
    24541 gaatgatagt cggtctcgtg gtattgaaat ggaatagtgt attcttgata gtcacaagct
    24601 tgactggcgt aagaggcgcc gatattcatt ttcagccgct taaaaagccg agtctgccag
    24661 cgcaaagaaa agacattgag caattcggca ttatccagaa taatgacctc ataattgcca
    24721 gatttcaccc aatcgtccgt agagcggatt tcatagataa attccaggga attattcgat
    24781 tctatagggg aataattcag atcaaggatt ccgcgccgga tttcacgtac ccaatgacca
    24841 cggggcgtgg gtttctgagg acttccagcg gtgacattat ccgaacgtcc gttatattct
    24901 ccggtaatta tcaaagccag gccaggaagc ataaaatcac ggtggcagaa ttgcaaagcc
    24961 agcccgccgc cctcacgttc atcatttttt ctattaatgg atttcggagt ttcatcctta
    25021 taaatatgat aaccaaacag agtgcgtacg aaggcatctt gatagtactt cacagcttca
    25081 tatttgcgct gagcgttata ataggtcccc aaaagccccc cgattatttt ctgattcttc
    25141 gaaatataac tcaagccgcc gcgcaattca gtattccggg cgataatctc gcattgggta
    25201 tagacatcct tcaaactttg ttcaactccg taattaatct caagcccgag atgcaggcgt
    25261 ttggttaagg catagtcata aagaaaatta atttgcgggc tattataagt tgtcgtgcca
    25321 gtggtcgtat ccagaaaagc aatatattcg tcataaatat ttttttccaa agagtggtag
    25381 agatcacgct gaatagtccg attaaacctg attccagtaa ccagggtgat gcgctcactg
    25441 agatgtttgg cggaatgaaa actaagggca taatcttcct gacgccgcgg gtcatatagc
    25501 cgtctaaaga cattattatc attggtagct tgcatctgat agaaatcgag attatccggg
    25561 taagaaatgc cgtagaaggc gggattgccg gataggtcat aaatagtgag attatactga
    25621 ttcattaaat tacgcggctg aatctcacgc ccaagcggat tgtcaatagc gaataatgga
    25681 gtaatgaata ccagtccaat cactactcct acaaataggc atttttttat aaaatcacta
    25741 ttcgttctca tggtgatggg tgatgatatc ctggggtagg cggtttaata attacgaagt
    25801 cgttaaagga attattggta tcaaagccag gcagaatccg ctcaaccgac tgcccgctat
    25861 acttctgatt gccaacgcca gcccaaccgg cgtctaaaat tttatcaatg tttttggttg
    25921 tctgttcgct agggctttta ctgttgtatt ccacgcaatc cactacggta ccgatatcga
    25981 gtccgtcggt cataaccgag tcgctaccat cggcgagtgc gatgacgttt gttgatagat
    26041 tgatcataaa atctacggtt ctacccggta cgatattagc taaatttgga acattaagat
    26101 tatctatgtc gccgaagtcc atagcattga caaattccca atcggctcca gaaagatcaa
    26161 cgctggtgct gtataactcc agccaattaa ctgcatcctg ggcaaggacg acaaattcac
    26221 ctggttgaac cggatattct cggccggtca gcggtgtgcc aggaaagcgg aaagcatagg
    26281 ttacagacgt caacatatga taaatccggc agacgatcat tccgtccaga tattgaacct
    26341 catccgagga attatacagc tcgataaatt gatcgtaaaa ccaattattc cgattttcgg
    26401 ggccagtgta ataaatctca ttgattttca agccaggccg gaggttagcg gtaatggtat
    26461 aaattgtatc ggtgcctatt ccggtttcgg agggagtaat cgccacaatc gtgtcaatca
    26521 gagtatagct tatgagcgaa tccagtcttc tattcccaaa actatcaatg aacggcaact
    26581 cgactttggc tttcacctgc ggatcataaa tcgcccaggg caaattttca aaaaccacta
    26641 cccctgaatc attagtagtt gcgcaataat catcaaggtt atagtcgcca gtatgaattt
    26701 tgacatcgac ctttgatagt ctctcatcgt aaccgagggt agagtcccga tataccaata
    26761 ccagttttaa agtataggtg ctatcgaatt ccgttggttt ctggtgaaaa cagttcgtta
    26821 aattcaagag taagataatg gttaaaagtg cagctttttt taccattgag cactcacttc
    26881 caatccgtaa aaaatcggcg gattatgttc gtttaacgaa ccatcgaaat ttttccatac
    26941 agcccggtca tccaatatat tattcacaaa caaggaaatt tccatacttt gggagagcga
    27001 cttggtaact cggaaattga agagccaccg accgccggta tcccgcagat aacgctcgta
    27061 ataataggtc atcccctgat accacaagta agtcacgcca ttttcagtct tataagtcga
    27121 attgctgcgg taaatacgct tgaaatgttc gagaggcacc tgttgagcat cgagtgtaac
    27181 ccagatgccg agccgattgc tgatataatt aatctgatag tctatgatga tcttttcact
    27241 ccagtatgac tccggcttgt accaaatatc ttcccaattt tccgggttag catcatagac
    27301 caagccagtt ttgccgtttt tattgaaccg ataggtgaca tttaaccggt attggaaatt
    27361 gtgaatgcgc tgggtgcgca tcgtaaattc gatcccattc gaacgactcc agcctcgatt
    27421 ttcgaaacgc tcccaagccg cggcggtaat cgtatccgga ttaatttcat agccataagg
    27481 ataactgacc gtaaccggca tattgtccga ggcttgataa tagccggtca gggaaaaacc
    27541 tatcagatcg ctgaatttcc aatcgagaga agc
//
LOCUS       DAOMJU010000021        27211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1599_length_27211_cov_15.570703, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000021 DAOMJU010000000
VERSION     DAOMJU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 27211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27211
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1599_length_27211_cov_15.570703"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PLO58_03420"
     CDS             <1..1203
                     /locus_tag="PLO58_03420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO73872.1"
                     /translation="FAFGMSFVPVMTVLPVFVRQVGGNNITVALVQVIWIIGVNFPQI
                     FIANYTRQLPYKKPFFLKTAALQRLPWLLMALLCFFIIKNVSSNMGLVFFFIAFGLAA
                     IAGAVNYPGWFDLISKITPVNLRGRLFALRTITGAILGIAGGWLVKVILSAQPFPANF
                     GYLFLLAFVMMTVSYVALIFLKEQEANASTESLNHFQYFRQLPNLIKSNHNFLNFLIA
                     DSLMTIGLIAQPFFIVNAMQRFGLDSASSGSFTMIYMLSMVILNLCSGSIGDRFGHRV
                     NMIIAAFSVLASCVLAITLRSLSLYYFVFVLSALNTSMIQVSRLPILAEICNEKERPT
                     YISISNMITSPFILLGLLGGYLVDLYGYDVIFAIAGLGGFLSLIWWVFYVAEPRRQNI
                     FVVESVER"
     gene            1232..>1599
                     /locus_tag="PLO58_03425"
     CDS             1232..>1599
                     /locus_tag="PLO58_03425"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQO73873.1"
                     /translation="MIKKSLIVYFVVMTIRGIGILQAQAITPDPRSKVDILLVVAHPD
                     DETAIGGQLAKWIFDDGKKIGVIYTNRGQGGGNSIGKEQYHSMGAIREIEARRALSAF
                     GIYNVWFLDGIDTPGQDVLHS"
     assembly_gap    1600..1699
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1700..2404
                     /locus_tag="PLO58_03430"
     CDS             <1700..2404
                     /locus_tag="PLO58_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73874.1"
                     /translation="DEALNAPGPQFDISAKSPSQGKPYIELAAQLHKFHQTQADVAQI
                     AEQAEQSGNWQPMIEWLEKFHLIYGKSVVKCEPTGDVFEGVSEKPADFHKNTGYISPV
                     ARGVKLQFGGIFDYYRHFWQVHDLQHLATIVSPEIAISVGSYFHVPLLITNGDKKTIR
                     LSVKPILPNGWQEVSGSGEYIIPAGESLPVQCFYFAPDNETDGFQPLKWQIIQDSKVL
                     DEIVMNVKLVEWALPQ"
     gene            2413..>3962
                     /locus_tag="PLO58_03435"
     CDS             2413..>3962
                     /locus_tag="PLO58_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73875.1"
                     /translation="MKNWLYLWLIIILFGNELNAQMLNPSIDRDDEPFCYFSKPTDQI
                     GVMDGREGTLITPEGYLYTGSGEMMFFTGNPPIPTEKRVKTLLNGYLPVIQYALNRDG
                     FDYRWTIFAATLDGKPESPLVNFVRVKIINRNSQQRTTWFAAGIRYQNEANTDWGIGD
                     NRFIRPAVPEQIGEYEQAGLEFRQDWLCLHQDDAVLYNDKLLYSFPPREKIRKFLTWK
                     MGYNEPPAEGEMTLYVLPTTPVGIVQYPLTMLPNEEVVLDFKFPYMPVDTVDEYSKWI
                     RQAQFDHYLNKTIQFWENILQEGMTIVLPEKKVTDTFNANLINSLIARDKIDGWYVQK
                     VNEFQYDNFWLRDAAYFTRMYDLTGYNNEANRILDFFPRWQREDGNFVSQGGQYDGFG
                     QTLWAYGQHVRMTDDRDFAEKVFPSVLKAIAWLESARKTDPLNLIPVTTPGDNENITG
                     HVTGHNFWTLIGIKNAVFIADYLGEGEKSQQLQKLYDDYYATLMRILAEITAQTGGYI
                     PPGLDGEGG"
     assembly_gap    3963..4062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4063..4392
                     /locus_tag="PLO58_03440"
     CDS             <4063..4392
                     /locus_tag="PLO58_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73876.1"
                     /translation="NAPTEFGEVNFTLTFKKNSATLVLKNNFKKAPAVIILHLPWFME
                     VRKIEIDGRSINQENNAVSLPVDTRQVDLEWSFKKNIENMSYDQTVEQYKSEYRRRYE
                     KFIREGD"
     gene            4401..5543
                     /locus_tag="PLO58_03445"
     CDS             4401..5543
                     /locus_tag="PLO58_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HQO73877.1"
                     /translation="MEHSKRIYSLDVFRGATIAAMILVNNAGSYDYVYPQLLHAEWHG
                     WTFTDWIFPFFLFMVGMSMVFSFESRLKHGDTHQQILRHSLKRLLILFALGLFINGFP
                     FGLIPGSQFSIATWRILGVLQRIGICYFFTSLIYLNVKPKGQWLWIGILLGIYWFALK
                     VIPVPGYGAGVLEPEGNLAWFIDKTLLGKHTWAFAPVFGFDPEGILSTIPAIASTLLG
                     VQAGRWLRSERSQEEKTAGMFVAGNLCLLLGIIMNIWLPINKNLWTSSYVVFINGWAL
                     NCFATIYWIVDVKGKTWGTKPFVIFGMNAITVYTLSELLANLLWAISWNRADGTFTTL
                     HDAIYENVFLPIASPVNASLLFAIAFVLMMYAVAWGMWKKRLFIKI"
     gene            5561..>7096
                     /locus_tag="PLO58_03450"
     CDS             5561..>7096
                     /locus_tag="PLO58_03450"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HQO73878.1"
                     /translation="MRFKISLFLLFFLFINGCSSSGINDLNQFVNPFIGTSGGGNTFP
                     GALVPWGMVSVSPHTDLKAPSGYIHGEPYFYGFGQVHLSGTGCADLGSVIITVTRGEV
                     KTEPEQYRCSYQNEQAKPGYYAVNLNEPDVKVEVTATTRCSFSQFTIGKEGNVNILID
                     AGRSLVERQGGSVRFVSPTEIEGYNRSGGLCGEDNQHQVYFVTRVSVTPQKFGTWIDD
                     QVSEDISRASDESSIGAWMTFTCQNGQIVNIKTGISYVSIANARQNLDAEITDWNFDK
                     IGRNAEREWRKQLSKIKVEGGSEAGLTKFYTALYHCLIHPNIISDVNGEFPLMGQTGV
                     GKYTDRERYSVFSLWDTYRTLHPLLTLIYPEQQSAIIRTMIDMYSESGFLPKWELAGN
                     ETYMMVGDPAPIVIADSYLKGIQDFDPDLALEAMLKPTLLDSGESAPPIRAGYHELLK
                     YGYIPFEQDTSQAWWVWGPVSTTLEYCLSDFCIGNMANMLGQNQIAEEFGKRASFYRN
                     LFDP"
     assembly_gap    7097..7196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7197..7923
                     /locus_tag="PLO58_03455"
     CDS             <7197..7923
                     /locus_tag="PLO58_03455"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HQO73879.1"
                     /translation="IAEEFGKRASFYRNLFDPKTRFMRPKLRNGQWLEPFDPLATEGS
                     GYWEGSGGPGYVEGNAWNYTWFVPHDVSGLIDQFGGYEPFRIKLERCFSEGHFTITNE
                     PDIAYPYLFSYIPGYEYRTAELIQSILTEEFVSEVDGLPGNDDAGAISAWLVFSALGF
                     YPACPAVDEYRLGIPLFQKVTIELDKEYYSGKQIIIERSKVSANPARLEEILIDGKKS
                     DSFGVTHKRLTEGVHLKFVDSQE"
     gene            7948..8964
                     /locus_tag="PLO58_03460"
     CDS             7948..8964
                     /locus_tag="PLO58_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HQO73880.1"
                     /translation="MKIIIFGSMLILIIVCLEYCQEEPEKPVTFTNPVVTDGADPWVI
                     RYQNFYYYCFSTGDRIWISCSRNLHEIGLAQPTLAWQPPKGNPWSEELWAPELHFFDN
                     KWYIYVAADDGANENHRMYVLESDNIDPQGHYVLVGKIADSTDCWAIDGTVLEHRGHL
                     YFIWSGWEGKVNVQQNLYIAPMSNPWTISGDRVLISEPEYEWEKVGRPLVNEGPEILK
                     KNDKIHIIYSASGSWTDDYCLGQLTFTGGDILSRASWIKKPEPVFSKTETVFGPGHAS
                     FVKSPDGREDWIVYHAAKYKGGGWDRDVRMQKFGWNNDDSPNFGAPVSAGVLLEVPSG
                     SYKK"
     gene            9014..10609
                     /locus_tag="PLO58_03465"
     CDS             9014..10609
                     /locus_tag="PLO58_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HQO73881.1"
                     /translation="MANIINSQSQAPSPSGELLFKPFFVQKGVGPHLGDLAFATDENG
                     DTFPSNMTIDADGIKISNTKGHKRFGINVRWNVEGFGYNDYTADNCGEFYELPIFGKQ
                     TVYNLNYEIAASRVARNRRRHNNFVKEGWQPSKETSAYLDLSEGLLEDAGRKPRLSED
                     CAQLAQKALHYALWGSEMMELEKAKWNIKRIGRRANFLFGCDARSYYEMPKDRFVEYF
                     TRLFNFAAVTYVVNGDPEMDIFEPVEGQLNFTHRDILCNVLRQNGIKVEGRLLFWFHK
                     WVTPEWLAHKNFDQLKKYVENHTREVLGHYGDRMAVWEVVNEFHDWANEVQLNPDQVV
                     ELTKFACEVARDTVPNIPLMINNCCPFAEYVHLGNWSGQKALYPQRTPWQFVRDLIAA
                     DVDFDIIGLQYYYQYRDLQDYIVNMERYESFKKRVQISEIGATSGPSEESVKNGSLGF
                     PTEPYSWRRPWDEELQADWAEALYLIGYSKSWIDSVLWFDFIDNGAYYDNGGLLSGRG
                     RHPKVAYERLIKLRQWLETSGTK"
     gene            10621..12291
                     /locus_tag="PLO58_03470"
     CDS             10621..12291
                     /locus_tag="PLO58_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HQO73882.1"
                     /translation="MPRITRRKFLKMATYAGLGGIVGGRALSAPLVLTPKRTKTNTEA
                     GELIFKPIIVQKGRGPHLLDWAYASDEHWDAFHSNITASNEGVKISDTEGTQKFGVDV
                     RWNVEGFGYIYITADNGGEFYELPPKGKQRELNLNFELAKSRVLRNRRRLELLRRDGW
                     QPSREVKELLALSDSLYEDAVKSQASAELCGDYAQRALYYAMLVGEKMELEKADFEIR
                     KIGYRPHFFIGCDSRAFYQMDPELFMERFTELFNYATITHYLLSGYYQDFEPIEGQKN
                     FNLRDAVFKELRQNGITVEGRPLFWFYNSTTPDWLRHKSYDQLLKYVESHTKEVVSHY
                     GEGMYAWEVVNEAHDWANELRLKPEEIVNVTKLACEVAKATNPKVHRLINNCCPFAEY
                     VQLKKWGELDAIYPQRTPHQFMQDLVDAGVDFTITGQQMYFPYRDLQDTIILIERLEK
                     FGRPVQLTEIGASSGPTKDSINTGQLGMPTEPYVWHRHWDEELQADWLEGVYTLAYSK
                     PWIEAANWYDFVDPYAWIKTGGLLRSPNGERKAAWQRLLKLKEQWKNL"
     gene            12315..13862
                     /locus_tag="PLO58_03475"
     CDS             12315..13862
                     /locus_tag="PLO58_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HQO73883.1"
                     /translation="MPNKTLIFKPYYIQKGRGPHLDEFAYLMDMNGDTFHSDVAVKNQ
                     GVTISNTKGIKKFTLNVRWNVEGYGYLFMPADNGGEFYSLPQFGSLQLNLNFELAKTR
                     IYRNRRRQANFNREGWLASRELSAMINLAENYLDDAIKNVQDDETCARFSQQALLYAL
                     WASEMLELEKARWNIAKNSARLDFLFGCDGRGYFQMDSELFLERFTEIFNYATITHYL
                     IGDFLDFEPVEGQKQFAERDRLLADLEKYKIQVAGRPLFWTHKWVTPDWLKNKTFPQL
                     LNYLEKHIRTVVGHYGNRIKVWEVVNELHDWANELFLNHEQTIELTKFACDVVRDVNP
                     NLKILINNCSPFGEYVQKGLWHELPARYPQRTPFQFTEQIIEAGTDFDAIGVQVYFTS
                     KTLADYIGVIERYEKLGKAIHLAEVGAPSRGITQEFLDEEPGDYSTLPYEWHRHWDEE
                     LQADWLEAIFTYAYSQPMIQAANWYDLIDGFSFLKNGGLLRSPKGERKAAVDRLKKLQ
                     EKWRILQ"
     gene            13863..15539
                     /locus_tag="PLO58_03480"
     CDS             13863..15539
                     /locus_tag="PLO58_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HQO73884.1"
                     /translation="MNNDPSLKKTLSRREFIRKSTMGIVGAAMFPSFLEKSNILAKTT
                     KPLLFKPFPHPWMPPLNFVYAADTNGDPFQSGIVVNGDGIVVPEDLGTRRFSVNCQWF
                     IEGFGNCFLSADNDGEFYSLKEMTGRNELILNHEFARSRIARNRQVMRRYQTQGTKFS
                     PEVIRLSGLAEEYLEDSAKYTAESEKCASIADKALYYALWTGENIELEHARQMISKNN
                     RQDEVYFGCESRQYVWAKNEEFTKRFVELFNFATVTHYIWDTWYELFEPREGVYNWGI
                     KDNIVRWLTEKNITIEGRPLFWFHPTVTPEWLKSKNYDQLRSYVDKHISDVVGHYGDQ
                     VLHWEVINEYHDWANIFNHTPDQITEIVRQVCDKTHEINPKVDRLINNCAPWGEYVAR
                     GRMARMEATRPLRTPYQFITDLLDAGVEFDTLGIQIYFGNRDLSDIMRMLERFEKFGK
                     PIYITEIGTTSGPTFETVVDRSMGISGRPYDWHRAWDEELQADWLEQVYTLYYSRLNI
                     KAINWYDFSNFRPFIVNGGLVTESCEPKQSFYRLKELLRSWNRLPDNIKK"
     gene            15550..16698
                     /locus_tag="PLO58_03485"
     CDS             15550..16698
                     /locus_tag="PLO58_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQO73885.1"
                     /translation="MTSKERLLIALKGGVADHVPVSPDISVMVPDRLDGRPFYEIHLD
                     GREHYGWTSATHGEVYVKAVKYFDMDGFYMYGGLKEVKVSSSLDFESETVDIPAGKLV
                     RRTWHTAKGDVTEEEIFFADESPWRSVKPIKNLQEDFEKFQLVMGKGDWEWEKEFRDK
                     QVIGDLGVYLGMIPVFQDWWFSNRDGGFENVFSDLLMEADYMQKVHEFWMNWALAYLR
                     AMIEAKPDVIMLGGSSASLSVSSPDIFRKYELPFIQQASSICKAANMPCHLHICGRSW
                     PLVQIVAEETDVTSMEPMEEPPTGNVDIAMVKKRYGRKLNIKGNINTTEFMMRATPQQ
                     VEDKCKRLIDDVGYDGGFVLSTGDQCGRDTPDANIFKMVEVAKTYGKY"
     gene            16731..19082
                     /locus_tag="PLO58_03490"
     CDS             16731..19082
                     /locus_tag="PLO58_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQO73886.1"
                     /translation="MRNIVKIITLIGLGLASTLAVELQWQTHNIEVWQPEQVIWGKID
                     TLTATSGVLYWKDGSEIFDINPSDSSFYIEVKLDEGENWFVATIENQGEPIYSDTLRL
                     QMKMPLKPILEAYATVADREVTLHGKIIENPDSNAVTFTWSEDERNSAIFGWNGTSDS
                     VLVFNVPEQVPAGEYYFDLILVTSGGDTTRARTFITVSFDSIKPFDIRRDHAAWIDSA
                     VVYEISPYAFVMEGTFQDIQQKLPELRELGINTIWLQPIFKTQWDEQGYDVIDYFTIR
                     SDYGSESDLQNLIKSAHTLEMKVLLDIVINHTSIYHPYAQHSIEYGSTSHYYDYYQRK
                     IDDAPYSQYYNYYKGFINYFWDELPNLNYDNPEVRNWMMTVCKYWVEKFDVDGYRFDA
                     VWGVNARLPEFTQNLRLELKRIKPEVMLLAEDKASWSMVFDQRFDVAFDWAPTEDWVS
                     QWYWEVDYSEDATKQFTIFNYSNPSERVNLLHQALTNYGQGYAANAKILRYMGNNDMI
                     PFIKNHNLAQTKMVSTLIFALNGMPMLYNGQEIGYNQHPYSSYQIFQRGKSIRSQDKY
                     GLFDHYRHLIQIRQRYSALRSDYFKEIPVVPAYYAFAFHRRSGDENLFSVINMMSEPK
                     NITISLPIEELHLDSTKTYYLSDLITGMTFSGKPENLSSVALTIPGYTTSLFLLADSM
                     VNVNIAPEEHPAIAEKFTLFQNYPNPFNSSTTIGYTIPQKSNVKLTVLDLLGREVMEL
                     VDSEQNPGEHQVLIRADQLASGCYFYRLKYEDQTLIRKMLLLK"
     gene            19236..21866
                     /locus_tag="PLO58_03495"
     CDS             19236..21866
                     /locus_tag="PLO58_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQO73887.1"
                     /translation="MITSCDKNPVVDLNGRWQLAPDSTGEITINSIDLIENWRMTEIP
                     GSWHRLHVDLLDYQGVVWYRKSFKLKSLPPKHRFLLNFGAVDYLAKVYVNRELVGEHE
                     GGYTPFSFDVTEQLQKGENEILVRVVDPVADEFGTDGISYWHIPHGKQNWYVQTSGIW
                     QAVQMAIKPEIYVSRIQVNSQISGAFQAKVFIENPKQVANPNKLVLTIRDPNGKVVFS
                     DKYEGNSSTSEAEFTGQINNPRLWTIGQPNLYTLEIRLGEDVLFDRFGFREIVVRDGK
                     FYLNGKPFYMIAALDQDFYPETVYETPSEEYLYDEMHKARELGLNTLRCHIKVPDPRY
                     LKVADELGLLVWYEIPNWDVFDESVKARSRKTLQEMLQRDWNHPSLAIISLINESWGL
                     DLNQANQRQWLKAEFDFAKSLANGRLIVDNSACWGNYHLKTDINDYHTYWAIPENHQK
                     FRETVQEVSDRPRWLFSPYGDAEETGQEVLMISEFGNWGLPKLPKKLPFWFDRQFLDN
                     LVTLPAGVTQRFGDYQYGRMFKNYNELAEFSQKAQAQALKWEIEEIRLQPELQGYVIT
                     EFTDINWECNGLLDMWRNFKADRQILAQVQQPDIIIPRLTKYGFWDNEEIDVQLSISH
                     YSNRDFSNVRIQGIYNGEIIGDKSIAIDSVGVYFVGSMKINSEPVKQPVSQRVKFRLL
                     SREGDVLAENFIEFTVYPSRKIENISPNSNLLICNSIDLKTMDHLKNGLDVICVIDSS
                     SVLPADFPFTITSRESDGYDGNWASNLNWVDDSHRPFNNLKSNKVLGFEAYKALPKYA
                     ITGIQPEQFVDVLAGMYVGWLHLNSAYLMQMRVGSGKLILTTFNLIQNYGDDPFTTTL
                     RDELVAYLQSEDCQPTINWK"
     gene            21888..23111
                     /locus_tag="PLO58_03500"
     CDS             21888..23111
                     /locus_tag="PLO58_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73888.1"
                     /translation="MNEIISKLEKLIEHNDGILRLKPAWVARDFLPPGRRLGLPEEMY
                     DLGERGGICERWVGSTTPADNRIKVPNEGLCCLDLEDGQEITLKAAVEIAPALIMGAD
                     YAKAHTGLGRLPKIFDYAYRLPYHLHQMAKDARKVGRNSKEEAYYFPEEVPLGPEPET
                     YFGVHPYISLEKRYELLLPSMQEWKDDSILLHSRAYRLKAGDGFHVPAGILHAPGSAL
                     TIELQEDSDVFAMMQAKVGGDIIIDKELLFKDVSEKERKNLAEKAILGMIDWELNGDP
                     YFYENRHTPPILIDSDQQSGSEQSWIFYNTHKFSGKKLIIRPGGKHVSVEKGVYNILA
                     WRGQGTYGNVEIEGLNFEKDELLICHQRAIYPIEVVNTGDRDLIIYKFFGPDINPDVP
                     MLTKYPKSPNACNIL"
     gene            23092..23685
                     /locus_tag="PLO58_03505"
     CDS             23092..23685
                     /locus_tag="PLO58_03505"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQO73889.1"
                     /translation="MPVISYKNEPFPDWSEVKSCKIIGLSKRQKLQLPSEFSETLLFV
                     LDGVAEISEGAEKRTIFSGDVLDQFFGEVWIEPKPLVELFQISGNWSNDRGSCGVFRL
                     RKSEHPRNDGDPVEYERNTVFDNHFHDCDEYWFIIKGKGMVVSEGESYAVQAGDCVIT
                     PAGEHHDFPIVQEPILAVWFEGSMKGKKRPGHLYREK"
     gene            23675..24994
                     /locus_tag="PLO58_03510"
     CDS             23675..24994
                     /locus_tag="PLO58_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQO73890.1"
                     /translation="MKNRKIKRLSIVGFVFLIVMANNCSKPKPRDANSITLTFWHSFV
                     ATTLPILEAMIADFEEENPGVKIQAQYVPTGDALVQKLITAIQSQTAPDIAWVHSDFL
                     DNLVNSRAIFSINELAGDDSLFYNQCLPDIFPQLLPPITFRDTLFALPMEATTLALLY
                     NRDWFRQIGLNPDHPPATWAELDSFTMQLCSDSNSDGQPERYGFYVPVFPASGPLNIW
                     MTMQWAPFVWQAGGEIFSNNQPTFASEAGVAALAFWSKIYHQQKFERFSMAHDMGFIS
                     QTVAMIMDGPWNLPRYRDYVKFDWGVAPLPAGSAGRASYLAGESLVVFKQCVNPEAAW
                     RFINWFLKPEVQRTFAEKSGYLPVRRSVLNDSDYVNFLEQFPQQKSFNEQIRYARGRS
                     LPDKFRVEVNQCIAEALESSIIGRIAPEKSLKVADEKIRRLLSSETE"
     gene            25058..25588
                     /locus_tag="PLO58_03515"
     CDS             25058..25588
                     /locus_tag="PLO58_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQO73891.1"
                     /translation="MSNILILYDSKTGNTAKMAELVALGARANGDHDVKILRIDDAKA
                     EDIRWCDGIALGSPTNMGIVSWKMKKFWDDLGDELWGKIEGKIGCAFSSSGGWGGGAE
                     ITCQSALMILINFGFLVFGVTDYVDEQLTLHYGVALAGEPRSAAEKAACLRLGERLAE
                     YLSKFKLQSDKNINEV"
     gene            25696..26778
                     /gene="ugpC"
                     /locus_tag="PLO58_03520"
     CDS             25696..26778
                     /gene="ugpC"
                     /locus_tag="PLO58_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HQO73892.1"
                     /translation="MASIELKQIVKEFDKGAPIIKNLDLTINDGEFLVIVGPSGCGKS
                     TTLRLIAGLEEVTRGEVYIDNRLVNNILPKDRDIAMVFQNYALYPHMTVYENMAFGLK
                     LRKYPKSEIRQRVKEAADILELGDLLNRKPKQLSGGQRQRVALGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRTEIKKLHHHLKTTMVYVTHDQVEALTMGDRIVVMKDGEIHQIGS
                     PSEIYQNPQDLFVAGFIGSPAMNFIPGVLHQGVFQSNTNNIKLILEKSPYKEMDNVAV
                     VLGIRPEDVHLNKQDAHYQEVRLTLEVIEPLGNESILYFSDEGHQITLRTQETNMPLS
                     IGASIPVFFNIDKVHIFDQITEKAIR"
BASE COUNT         7667 a         4950 c         6412 g         7882 t
ORIGIN      
        1 ttcgccttcg gaatgagctt tgttccggtt atgaccgtac taccggtgtt cgtccgacag
       61 gtaggcggta ataatattac cgtggcattg gttcaggtaa tttggattat cggagttaat
      121 tttcctcaga tttttattgc caattatacc cgccaattac cttataaaaa gccatttttc
      181 ttaaaaactg cggctttgca acgattaccc tggttattaa tggcattgct ctgttttttt
      241 attataaaaa atgtctcgtc caacatggga ttagtctttt tttttattgc gtttggattg
      301 gcggcaattg caggcgcagt aaactacccc ggttggtttg atttaatttc aaagattact
      361 ccggttaatt tgcgggggcg gttattcgcc ttgcgaacta tcactggtgc aattttagga
      421 atagcgggtg gctggttggt taaagttatc cttagcgccc aaccgtttcc cgctaatttt
      481 ggctatttat ttctattagc gtttgtaatg atgaccgtgt cttacgttgc cttaattttt
      541 ctaaaggaac aggaggcgaa cgccagcact gagtcattga atcattttca atatttcaga
      601 caattgccca acttgataaa aagtaatcat aactttctga atttcttgat tgctgatagt
      661 ctgatgacta ttggactaat tgctcagccg ttttttatcg tgaatgcgat gcaacgtttc
      721 ggcttagatt ccgcatcaag tggcagtttt acaatgatat atatgcttag tatggtgatc
      781 ttgaatctct gctccggttc catcggtgat cggtttggtc accgagtcaa tatgataata
      841 gcggcatttt cagttttagc cagctgcgtt ctggcaataa cactgcgctc cttgtcttta
      901 tattattttg tgttcgtgtt atctgcttta aatacgagta tgattcaagt ttctcgtttg
      961 ccaattctgg cagagatatg caacgaaaag gagcgcccga cttatatatc aatttctaat
     1021 atgattacca gtccgtttat tttattgggt ttgttgggag gttatttggt ggatctatat
     1081 ggttatgatg taatctttgc aattgctggc ttgggaggat ttctttcgtt aatttggtgg
     1141 gtattttatg tcgcagaacc acgccgccaa aatatttttg tggttgaatc ggtggaaaga
     1201 taatgaacag agtaatatta tgacaggaca aatgataaaa aaatcgctga tagtttattt
     1261 tgtggttatg acaatcaggg gaatcggaat tctccaagct caggcaatta cacccgatcc
     1321 acgttccaaa gtagatattc ttttagtcgt tgcccatccc gatgatgaaa ctgcaattgg
     1381 tggtcagctg gcaaaatgga tttttgacga cggtaaaaaa attggggtga tttatactaa
     1441 tcgcggccag ggtggcggta attcgattgg taaagagcaa tatcactcaa tgggggctat
     1501 tcgggaaata gaagcgcggc gcgcattaag tgcctttggc atttataatg tttggtttct
     1561 cgatggcata gacacacccg ggcaagatgt tttgcattcn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnng atgaagcgtt aaatgctccc ggaccacaat ttgatattag
     1741 tgccaaatca ccttcgcagg gaaaacctta tatcgaatta gccgcgcaac tgcataaatt
     1801 ccaccaaacc caggcggatg tagctcaaat tgccgaacaa gcggagcaat ctggaaattg
     1861 gcaaccaatg atcgagtggc tggaaaaatt ccatctgata tacggtaaat ccgttgtaaa
     1921 atgtgagcca accggtgatg tttttgaagg tgtcagcgaa aaaccagcag attttcataa
     1981 aaacactggc tatatttcgc cagtagcgag aggtgttaaa ctacaattcg gtggtatttt
     2041 cgattactac cgccatttct ggcaggtgca tgatcttcag cacttggcga caatagtctc
     2101 gccggaaatt gccatttcgg tggggagtta ttttcacgtg ccactcctga taacgaacgg
     2161 tgataaaaaa acgataagac tgagcgtcaa accgatttta ccgaatgggt ggcaggaagt
     2221 cagtggaagc ggtgagtata tcattcctgc cggagaaagt ttaccggtac aatgtttcta
     2281 ttttgctcca gataatgaga cggacggatt tcagccgttg aagtggcaaa taattcagga
     2341 tagtaaagta ctcgatgaaa tcgtaatgaa cgtaaagctg gtagaatggg cactaccgca
     2401 ataggataaa gcatgaaaaa ttggttatat ctctggttga ttataatact ttttggaaat
     2461 gagttgaatg cccagatgtt aaacccatcg attgatcggg atgacgaacc tttttgttat
     2521 tttagtaaac cgactgatca gatcggagtt atggatggtc gcgaggggac gttgattact
     2581 cctgaaggat atttatatac cggttcgggt gaaatgatgt ttttcaccgg caatccgcca
     2641 ataccgactg aaaaacgggt caaaactttg ttaaatggtt atctaccggt cattcaatat
     2701 gcactaaatc gtgatggttt cgattaccga tggacaattt ttgctgcaac tttggatggt
     2761 aagccagaaa gtccgctggt caattttgtt cgggtaaaaa ttattaatcg aaattctcag
     2821 caaaggacta cctggtttgc ggctgggatt cgttaccaga atgaggcgaa taccgattgg
     2881 gggattggtg ataatcgttt tataagaccg gcggtaccgg aacaaatcgg tgaatatgaa
     2941 caagcggggc tggaattccg tcaggactgg ctttgtttac atcaagacga tgcggtttta
     3001 tataatgata aattactcta ctcatttccg ccacgtgaga aaattcgcaa atttttgacc
     3061 tggaaaatgg gctataatga accaccggca gaaggcgaga tgacccttta tgttctccca
     3121 acaacaccgg tggggattgt acagtatcct ttgacgatgc tcccaaacga ggaagtcgtt
     3181 cttgatttca aatttcccta tatgccggtt gatacggtgg atgagtattc aaaatggatt
     3241 cggcaggcac aatttgatca ttatcttaat aagacgattc aattctggga aaatattctg
     3301 caagagggaa tgacaattgt cttaccggag aaaaaggtaa ccgacacttt taatgccaac
     3361 ctaatcaata gcttaattgc acgtgataaa attgacggct ggtatgttca aaaggtcaat
     3421 gaatttcaat atgataattt ctggttacgg gatgcggcgt attttactcg aatgtacgac
     3481 cttaccggtt ataataacga agccaatcgt atattagatt ttttcccgcg ctggcaaaga
     3541 gaggatggta attttgtttc gcaaggcgga cagtacgacg gatttgggca gacactctgg
     3601 gcgtatggtc agcacgtccg tatgacggat gatcgagact ttgctgagaa ggtgtttcct
     3661 tcggtattaa aagccattgc ctggctcgaa agcgcccgaa aaactgatcc cttgaacttg
     3721 attccagtaa cgactcctgg tgataacgaa aatattaccg gacacgttac cggccataac
     3781 ttctggacat taattggaat aaaaaacgcc gtttttatag ctgattattt gggagaaggg
     3841 gaaaaatctc aacagttaca aaagttatat gacgattatt atgcgacttt aatgcgaatc
     3901 ctggcagaaa ttaccgccca gaccggaggt tatattcctc ccggattgga tggagaaggc
     3961 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatgcacc cacggaattc
     4081 ggtgaagtta atttcacctt aacatttaaa aagaattcag cgactttagt attgaaaaat
     4141 aattttaaaa aagcaccagc ggtgattata cttcatttgc cctggtttat ggaagtgcga
     4201 aagattgaaa tagacggacg ttcaattaac caagaaaata atgcggtctc tctcccagtt
     4261 gatactcgtc aagttgattt agaatggtct tttaaaaaga acatagaaaa tatgagttat
     4321 gatcagacgg ttgaacagta taaaagcgag taccgaagac gttatgaaaa atttatacga
     4381 gaaggtgatt aagagggaag atggaacact ctaagcgtat ttactcccta gatgtctttc
     4441 gcggtgcgac tatagcggct atgattctgg tcaataatgc cggttcttat gattacgttt
     4501 atcctcaatt attacacgcc gagtggcatg gatggacttt taccgactgg atctttcctt
     4561 tttttctatt tatggttggt atgtcaatgg tattttcctt tgagagccga ctgaaacacg
     4621 gtgacactca tcagcagatt ctacgacatt ctttgaagcg cttgctgatt ctatttgcct
     4681 taggattatt cattaatggc ttcccgtttg ggcttattcc cggatcgcaa ttttcgattg
     4741 ctacctggcg tattttagga gttttgcaac ggatcggcat ctgttatttt ttcacgagtc
     4801 ttatttatct gaatgtaaaa cctaagggac agtggctctg gattggtatt ttgttgggca
     4861 tatactggtt tgctctcaaa gtgattccgg tccccggtta cggagcggga gtccttgaac
     4921 ccgaaggaaa cctggcttgg tttatagaca aaaccttatt gggcaaacac acctgggctt
     4981 ttgcaccggt attcggcttt gatccggaag gcatcctaag caccattcca gcgattgcgt
     5041 caactttatt aggtgttcaa gccggtcgct ggttgcgcag tgaacgtagt caagaagaga
     5101 aaacggccgg aatgtttgtc gctggcaatt tatgtctatt gctcggtatc ataatgaaca
     5161 tctggctacc gattaataaa aacctgtgga ctagctccta tgtagttttt ataaacggtt
     5221 gggccttgaa ctgcttcgcc acaatttatt ggattgtgga tgtgaaaggt aagacctggg
     5281 gcaccaagcc gtttgttatt tttggtatga atgcaattac agtttatact ttatcggaat
     5341 tgctggctaa tctattatgg gcaatatcct ggaatagagc tgatggtacg tttacaacgc
     5401 ttcatgatgc tatttatgag aacgtatttt taccgatagc cagtccggtc aatgcttctt
     5461 tactatttgc aattgctttt gtgttgatga tgtatgccgt tgcctggggt atgtggaaaa
     5521 agagattatt catcaaaatt tgaaaattag agaattactt ttgcgcttta agatatcatt
     5581 atttttactt ttttttctgt ttataaatgg atgttcatct tctgggatta acgatcttaa
     5641 tcaatttgtc aatccattca ttggaacgag cggtggcggt aacactttcc cgggggcgct
     5701 ggttccctgg ggaatggtgt cggttagtcc gcatactgat ttaaaagcgc catcgggcta
     5761 cattcacggc gagccctatt tttatggttt tggacaggtt catcttagtg gaaccggatg
     5821 tgccgatttg ggcagtgtga ttattactgt aacgcgaggc gaagttaaaa ctgagccgga
     5881 acagtaccgc tgttcgtacc agaatgagca ggctaaaccg ggctactatg cggtgaattt
     5941 gaatgaaccg gatgtgaaag tagaagtgac ggctacgacg cgctgtagtt ttagtcagtt
     6001 taccattggt aaagagggaa atgttaatat tttgattgat gccgggcgca gtttagtgga
     6061 gcgacaagga ggtagcgttc gttttgtttc gcctactgaa attgagggtt ataatcggtc
     6121 aggtggttta tgtggtgaag ataatcagca tcaggtatat tttgttacga gggtaagtgt
     6181 aactcctcag aaattcggta catggataga cgatcaagta agtgaagata tttcacgagc
     6241 ttcggatgag agtagtatcg gagcctggat gacttttaca tgccagaacg ggcaaattgt
     6301 gaatattaaa actggaattt cctatgtcag catcgctaat gcccggcaaa atctagatgc
     6361 cgaaattact gattggaatt ttgacaaaat tgggcggaat gcagaacgcg aatggcgaaa
     6421 acagttatca aaaattaagg tagaaggcgg tagtgaagcg ggtttgacga aattttatac
     6481 ggcgctctac cattgtttaa ttcatcccaa tattattagt gatgtcaacg gcgaatttcc
     6541 tttgatgggg caaaccggtg tcggtaaata taccgaccga gaacgctact cggttttttc
     6601 cctctgggat acttatcgca ctttacatcc tcttttaacg ttgatttatc ctgaacagca
     6661 atcagcgatt attcggacga tgattgatat gtattcggaa tctggctttc tgccaaagtg
     6721 ggaactcgct ggtaatgaaa cttatatgat ggtcggtgat cctgcaccaa tcgtaatcgc
     6781 cgatagttat ctaaaaggga tacaagattt tgatcctgat ttagctttgg aggcaatgct
     6841 caaaccgacg ttactggatt ctggcgaatc agccccgcca attcgggctg gttaccacga
     6901 actgctaaaa tatggttaca tcccatttga acaagatacg agtcaagcct ggtgggtttg
     6961 gggaccggtt tcgacaacgc ttgaatactg cctatctgat ttttgtatcg ggaatatggc
     7021 aaatatgctg ggtcaaaatc aaattgcaga ggagttcggt aaaagagcct cattttatcg
     7081 aaaccttttc gatccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaatt
     7201 gcagaggagt tcggtaaaag agcctcattt tatcgaaacc ttttcgatcc gaaaacccga
     7261 tttatgcgcc ccaaactgag aaatggtcag tggttggaac cttttgatcc gctggcaacc
     7321 gaaggctctg gttactggga aggttccggc ggtccgggct atgtggaagg caatgcctgg
     7381 aattatacct ggtttgtgcc gcatgatgtt tcaggactta ttgaccaatt tggtgggtat
     7441 gaacctttca gaattaaatt ggagcggtgt ttttccgaag ggcattttac cataacgaat
     7501 gaacccgata ttgcttatcc ttatctgttt tcttatatcc cgggttatga atatcgcact
     7561 gctgaactaa ttcagagtat tttgacagaa gagtttgtat cggaagttga tggtttaccg
     7621 ggcaatgatg acgctggtgc gatttcagcc tggttagttt tctcggcgct gggattctat
     7681 ccggcttgtc cggcagtgga tgagtaccga ctgggaattc cgttgtttca gaaagtaact
     7741 attgaattgg acaaggaata ttattccggg aaacagatta ttatagaaag atccaaagta
     7801 tcagccaatc cagcgcgttt ggaagaaata ttaattgacg gcaaaaaatc agactcattt
     7861 ggagtaactc acaaacgtct gacggaaggc gtccatttaa aatttgtaga tagtcaggag
     7921 taatatcttt tgaaccttgg ccattggatg aagattatca tattcggatc aatgctgatt
     7981 ttgattattg tctgtctgga atattgccaa gaagaaccag aaaaaccggt cacttttact
     8041 aatccagttg tcaccgatgg tgccgatccc tgggtaatca gataccaaaa tttttattat
     8101 tactgttttt caacgggaga tcgcatctgg attagttgtt ccaggaatct acatgaaatt
     8161 ggattagcgc aaccaacgct tgcctggcag ccgccgaaag gtaatccctg gtcggaagaa
     8221 ttatgggcgc cggagttgca tttctttgat aataaatggt atatctacgt cgcggctgac
     8281 gatggcgcga atgaaaatca ccggatgtat gttctggaaa gtgataatat tgatccgcaa
     8341 gggcattatg ttttggtagg gaaaattgcc gattcaacgg attgctgggc aattgacgga
     8401 accgttttag aacatagagg tcatctctat ttcatttggt cgggttggga agggaaagtc
     8461 aatgttcagc agaatcttta cattgcaccg atgagcaatc cctggacgat cagtggcgat
     8521 cgagtgctga tttctgaacc tgaatatgaa tgggaaaagg tcggtcgtcc cttggtgaat
     8581 gaggggccag agatattaaa gaaaaatgat aaaattcaca ttatttattc tgctagcggg
     8641 agttggacgg atgattattg tttgggtcaa ttaactttta cggggggaga tattttgtcg
     8701 cgcgcgagtt ggattaaaaa gcccgagccg gttttttcga aaacggaaac tgtattcggt
     8761 ccgggacatg cctcgtttgt caagtcgccg gatggtcggg aagactggat cgtttaccac
     8821 gccgctaaat acaagggtgg tggctgggat cgtgacgtac gaatgcagaa atttggttgg
     8881 aataacgatg attcgcctaa ttttggcgcg cccgtaagcg ctggagtttt attagaagtc
     8941 ccttctggat catataagaa gtagttgaat aggatggaaa tcaagtaata attcggggag
     9001 atatatagcg gttatggcaa atatcataaa ttctcagtcg caagcaccca gtccttctgg
     9061 cgaactgctt tttaaaccat tctttgtaca aaagggtgtt ggtcctcatc tcggtgattt
     9121 agcatttgcc actgacgaaa acggcgatac ttttccatcg aatatgacca ttgatgccga
     9181 cggaataaaa atttcgaata ccaaaggtca taagcgcttt ggaatcaatg ttcgctggaa
     9241 tgtggaaggt tttgggtata acgattatac cgctgataat tgtggtgaat tttacgagtt
     9301 gccgattttc gggaagcaaa ccgtctataa tttgaattac gagatcgccg ccagtcgggt
     9361 ggcaagaaac cgccggcgtc ataacaattt tgtcaaagaa ggatggcagc cttcaaagga
     9421 gacatctgcc tatttagatt tgtcggaagg attgttagaa gatgccggcc gaaaaccgcg
     9481 cttaagcgag gactgtgctc aactggctca aaaagctctc cactatgcct tatggggtag
     9541 cgagatgatg gaattggaaa aagccaaatg gaatattaaa cgaatagggc gccgagcgaa
     9601 tttccttttc ggttgtgatg ctcggtcata ctatgaaatg cccaaagatc ggtttgtgga
     9661 atattttact cgtcttttta attttgcagc cgtgacgtat gtcgtcaatg gcgatccgga
     9721 aatggacatt tttgaaccgg ttgaagggca gctgaatttt acccatcgcg atatactctg
     9781 caatgttctg cgacagaatg ggataaaagt agaagggcgt ttgctgtttt ggtttcataa
     9841 atgggttacg cctgagtggt tagcgcataa gaattttgac caattaaaga agtatgtcga
     9901 gaatcatacc cgggaagtgc tcggtcatta tggggatcga atggctgtct gggaagtggt
     9961 gaatgaattt cacgattggg ctaatgaagt ccaattaaat ccagatcaag ttgtcgaatt
    10021 aacgaaattt gcctgtgaag tagcgcgcga tacggttccg aatattccgc taatgatcaa
    10081 caattgttgt ccgtttgcag aatatgttca cttaggaaat tggtcggggc aaaaagccct
    10141 ttatccccaa cgcacaccct ggcaatttgt gcgtgacctt atcgccgccg atgtggattt
    10201 tgacattatc ggactgcaat attattatca ataccgtgat ttacaagatt acattgttaa
    10261 tatggagcgc tatgaatctt tcaaaaagag agtccaaatt tcagagattg gtgccaccag
    10321 tggtccttcg gaggaatcgg tcaagaacgg tagtttagga tttccgactg aaccttattc
    10381 atggagacgc ccctgggacg aagaattgca agcggactgg gcggaggctt tatatttaat
    10441 tggttatagt aagtcttgga ttgattcggt gctatggttt gattttattg ataatggcgc
    10501 ctattatgac aatggcggtt tactaagcgg ccgaggtcgt caccccaaag tggcttacga
    10561 acgacttata aaattgcgcc agtggttaga aacatcagga acaaaataaa ggatagaatt
    10621 atgccaagaa taacccgacg aaagttttta aaaatggcta cgtatgccgg tctgggcgga
    10681 atagttggtg gacgagcttt atcggcacca cttgttctaa cgcccaaacg cacgaaaact
    10741 aatacggaag ccggagaatt aatttttaaa ccgattattg tccaaaaagg gcgaggacct
    10801 catttattgg attgggcata tgccagtgat gaacattggg atgctttcca ttctaacatt
    10861 accgcatcca atgaaggggt gaaaatttct gatactgagg gtacccaaaa attcggcgtt
    10921 gatgttcgct ggaatgtgga aggtttcggc tacatctaca tcactgctga taacggtggc
    10981 gaattttatg aattgccccc aaaagggaaa cagcgcgagt tgaatctcaa ttttgaatta
    11041 gctaaaagcc gcgtgttgcg caatcggcgc cgtttggaat tactccgacg tgatggctgg
    11101 caaccgtcac gagaagtaaa agagcttctg gcgctatccg acagtctcta tgaggatgcc
    11161 gtaaaatcac aggcatcagc ggaactctgc ggtgactatg ctcagagagc attgtattat
    11221 gccatgttag tcggcgagaa gatggaactg gaaaaagctg attttgaaat tcgcaaaatt
    11281 ggttaccggc cgcatttttt catcggttgc gattcacggg ctttttacca gatggacccg
    11341 gaactattta tggagcgatt taccgagctt ttcaactatg ccacgattac tcattatctg
    11401 ctcagcggtt attatcagga ttttgagcct atagaggggc agaaaaattt caatttacga
    11461 gatgccgttt ttaaagagct acgccaaaac gggattacgg tagaagggcg tccacttttc
    11521 tggttctata attccactac tccggattgg ttacgccata aatcctatga tcagttactg
    11581 aaatatgtcg aaagtcatac caaagaggtg gtcagtcatt atggtgaagg tatgtatgcc
    11641 tgggaagtag ttaatgaagc tcatgactgg gcgaacgaac tacgcttaaa acctgaagag
    11701 attgtcaatg ttaccaaact ggcttgtgaa gttgccaaag ccactaatcc aaaagttcat
    11761 cgactgatta ataactgctg tccttttgct gagtatgttc agttaaaaaa atggggtgaa
    11821 ctggatgcca tctatcctca gcgcacgcct catcaattta tgcaggacct agtagatgcc
    11881 ggagtagatt ttacaattac tggtcaacaa atgtattttc cctatcgtga cctacaggat
    11941 acaattatcc tgattgagcg actggaaaaa ttcggacggc cggtgcagtt gactgaaatt
    12001 ggggcgtcct ccggtccgac taaagactcc atcaataccg gtcagttagg aatgcccacc
    12061 gaaccttatg tctggcatcg ccattgggat gaagaattgc aggctgattg gttagaagga
    12121 gtatatacat tagcctatag taaaccgtgg atcgaagccg cgaattggta cgatttcgtt
    12181 gatccttacg cttggataaa aacgggtggt cttctgcgca gtccaaacgg tgaaagaaaa
    12241 gccgcctggc aacgtttgtt gaagctcaaa gaacagtgga aaaatttgta gtagtgccca
    12301 agattgtgtg aaaaatgccg aataagacat tgatatttaa accatattac attcaaaaag
    12361 gacgcggtcc gcatcttgac gagttcgctt atttgatgga tatgaatggt gacacctttc
    12421 attccgatgt tgctgttaaa aatcaaggtg ttactatcag taatacgaag ggaattaaaa
    12481 aatttacctt gaatgttcgc tggaatgttg aaggttatgg ctaccttttt atgcccgcgg
    12541 ataatggcgg tgaattttat tctttgcccc agttcggttc gctccaactt aatttaaatt
    12601 ttgaattagc aaaaacccgg atctatcgta atcggcgtcg gcaagcaaat tttaaccgtg
    12661 aaggttggtt ggcatctcgc gaattgtctg ctatgattaa tctggcggaa aattatttag
    12721 atgatgcgat caaaaatgtc caggatgatg agacctgcgc tcgattttcc caacaagctc
    12781 tcctttatgc cctttgggca agtgaaatgt tggaattgga gaaagcgcgc tggaatattg
    12841 ctaaaaatag tgcccggctc gatttcctat ttggttgtga tgggcgtggt tattttcaga
    12901 tggatagtga attatttctg gaacgattta ccgaaatatt taattatgcg acaattactc
    12961 attacctgat tggtgatttc cttgatttcg agccagtcga agggcagaag caatttgccg
    13021 aacgtgaccg tctcttagcc gatttagaaa agtacaagat tcaggttgca ggtcgtccgt
    13081 tattttggac acataaatgg gtaacaccgg attggttgaa aaataagact tttcctcaat
    13141 tgctgaatta tctggaaaag catattcgga cagttgttgg ccactatgga aataggataa
    13201 aagtatggga ggtggtcaat gaattacatg actgggcgaa tgaactattt ttgaaccatg
    13261 aacagacgat tgaattgact aagtttgcct gtgatgttgt tcgcgatgtc aatcccaatt
    13321 tgaaaatatt aatcaataat tgtagtccct ttggtgaata tgtccagaaa ggcttgtggc
    13381 atgagcttcc ggctcgatac cctcagcgaa cgccattcca atttaccgag caaatcatcg
    13441 aagctggtac cgattttgat gcaatcggtg tgcaggtcta ttttacctca aagacattag
    13501 ccgattatat cggcgtcatc gaacgctatg agaagctcgg taaagcgatt catttggcgg
    13561 aagtcggggc accgtccaga ggaatcacgc aagagtttct ggatgaagag cctggtgatt
    13621 attctacttt accctatgaa tggcatcgtc actgggacga agaacttcaa gccgactggc
    13681 tggaagctat ttttacctat gcttacagtc agccaatgat tcaggcggca aactggtatg
    13741 atttgattga tggattttcc ttcttgaaaa acggtggctt attgcgttca ccgaaaggtg
    13801 aacggaaagc ggccgtcgat cgcttgaaaa aattacaaga gaaatggagg attttacaat
    13861 aaatgaataa tgatccatcc ttgaagaaaa ctttatcacg acgcgagttc attcggaaaa
    13921 gtacaatggg tatagttgga gccgcgatgt tcccgtcttt tctggaaaaa tcgaatattc
    13981 ttgccaaaac cactaagcca ttgttattta aaccttttcc tcatccctgg atgccaccgc
    14041 ttaattttgt ttatgcggct gacaccaatg gcgacccttt tcaatctggt atcgtggtaa
    14101 acggagatgg aattgtcgta cctgaggatt tgggaacccg acgcttcagt gtgaattgcc
    14161 agtggtttat cgaaggcttt ggaaactgtt ttttaagtgc cgataatgac ggagaatttt
    14221 attctttaaa ggaaatgacg ggacgaaatg aacttatctt gaatcacgaa tttgccagaa
    14281 gccgaatagc ccgaaatcgc caagttatgc gacgctatca aactcagggt actaaatttt
    14341 caccggaagt aattcgtttg tctggtttag cagaggaata tctggaagat tccgctaaat
    14401 ataccgccga aagtgaaaag tgtgcctcta tagcggataa agctctttac tatgctttat
    14461 ggacgggaga gaatatcgaa ttggaacatg cccgccaaat gatttctaaa aataatcgcc
    14521 aggatgaagt ttactttggc tgtgaatccc gccagtatgt ttgggcgaaa aatgaagaat
    14581 tcaccaagcg ttttgtcgaa ttattcaatt ttgctacagt aactcattat atctgggaca
    14641 cctggtatga attatttgaa ccgagggaag gagtttataa ctgggggatc aaggataaca
    14701 tcgttcgctg gctaaccgag aaaaatatta ctatagaagg ccgtccactc ttttggtttc
    14761 atcctactgt aactccggaa tggttgaaaa gtaaaaatta tgatcaattg agaagttatg
    14821 tagataaaca tattagcgac gttgtcggtc attatggcga tcaggttttg cattgggaag
    14881 tcataaacga atatcacgac tgggcaaata tcttcaatca tactcccgat caaatcacgg
    14941 aaatcgttcg ccaagtatgt gataaaaccc atgagattaa tcctaaagta gatcgcctga
    15001 tcaataattg tgcaccctgg ggtgagtacg ttgccagagg tagaatggcg cggatggaag
    15061 cgacccggcc tttgcgcacc ccttatcaat tcatcaccga tttactggat gcaggcgtgg
    15121 aatttgatac actgggaatt caaatttact ttgggaatcg cgatctatcg gacataatgc
    15181 ggatgctgga aaggtttgag aaatttggga aaccgatata tatcactgaa attggtacaa
    15241 cctcaggtcc gacttttgaa accgttgtgg accgcagtat gggcatctct gggagaccat
    15301 atgattggca ccgcgcctgg gacgaagaat tacaggctga ttggttggag caagtttata
    15361 ctctttatta cagcaggctc aatattaaag ccatcaactg gtatgatttt tccaattttc
    15421 gcccgtttat tgtcaatggc gggctggtga ccgaaagttg cgaaccgaag cagtcgtttt
    15481 atcgcttgaa ggaattattg aggagctgga atcgtttgcc tgataatatt aaaaaatagg
    15541 acgaaaataa tgacatcgaa agaacgttta ttgattgctt taaaaggtgg agtagcagac
    15601 cacgttccgg ttagtccgga tatatcggta atggtgccag accgactgga tgggcgcccc
    15661 ttttatgaaa tccatttgga tggtcgtgaa cattatggct ggacttcagc cacccacggt
    15721 gaagtttatg tcaaagctgt aaaatatttc gatatggatg gtttttatat gtatggcggc
    15781 ttgaaagagg ttaaagtatc aagttcgctg gattttgaat cggagacggt agatatacca
    15841 gctggaaaac tagtccgacg tacctggcat acggctaaag gtgatgtaac cgaagaagaa
    15901 atattttttg ccgatgaatc cccttggcgg agtgtcaaac cgattaaaaa cttgcaagag
    15961 gatttcgaga aattccagtt ggtaatgggg aaaggtgatt gggaatggga aaaggaattt
    16021 cgtgataagc aggtgattgg tgatttgggc gtctatttag ggatgatacc agtcttccag
    16081 gattggtggt ttagcaaccg ggatggtggg tttgagaacg tattttccga cttgctgatg
    16141 gaagctgatt atatgcaaaa agttcatgaa ttctggatga actgggcatt agcctatctg
    16201 cgagctatga ttgaagccaa accggatgtg attatgctgg gtggttccag tgccagtctt
    16261 agcgtaagtt ctcccgatat tttccggaaa tatgagttac cttttattca gcaggcttcc
    16321 agcatttgta aagcggcgaa catgccttgc catttgcata tttgtggtcg ttcctggcca
    16381 ttggtgcaaa tagtagcaga agaaacggac gttacttcga tggaaccaat ggaagaaccg
    16441 ccgactggca atgtggatat tgctatggtt aagaaaagat atggacggaa attgaatatt
    16501 aaaggcaata ttaatacaac cgaatttatg atgcgagcga caccgcaaca ggtcgaagat
    16561 aagtgtaaac ggctaattga tgatgtcggc tatgacggtg gttttgttct ttcaaccggt
    16621 gatcagtgtg gacgtgatac cccagatgcc aatatattta agatggtgga agtagctaaa
    16681 acgtatggaa aatattagta cggtaaaaaa aagtccaaaa gtgcattgcg atgcggaata
    16741 tagttaaaat aattacactg attgggcttg gcttggcttc gacgttagca gtagagctcc
    16801 agtggcaaac tcataatatc gaggtatggc aaccggaaca agtaatttgg ggaaaaattg
    16861 atacactgac tgctacatct ggggttttat attggaaaga tggaagtgaa atcttcgata
    16921 ttaatccttc cgattccagc ttttacatcg aagtaaaatt agatgagggt gagaattggt
    16981 ttgtagcaac gattgaaaat caaggtgaac caatttattc tgacacatta cgcttacaga
    17041 tgaaaatgcc cttaaaacca attctggaag cctatgcgac ggtcgctgat cgcgaagtga
    17101 cattacacgg taaaatcatc gaaaatcccg attcaaatgc ggttacattt acctggtctg
    17161 aagatgagcg aaattccgct atattcggct ggaatgggac gagcgattcg gttttggttt
    17221 ttaacgtgcc agagcaggtt cccgccggtg aatattattt cgatttgatc ttggttactt
    17281 ccggtggaga caccactcgt gctcgcacct ttatcacagt aagttttgat agtattaaac
    17341 cgtttgatat tcgccgcgat catgcggctt ggattgattc ggccgtggtg tatgaaatta
    17401 gtccttacgc ctttgttatg gaaggcacct tccaagatat tcaacagaaa ctgcccgaat
    17461 tgcgggaatt ggggattaat accatttggc ttcagccgat tttcaaaact cagtgggatg
    17521 agcaaggcta cgacgtaatt gactatttta ctatacgttc ggattatggt tcggaaagtg
    17581 acttgcagaa tttgatcaag tccgctcaca cgctggagat gaaggttctt ctagatattg
    17641 tcatcaacca cacgtctatt tatcatccgt atgcccagca cagtattgaa tatggtagca
    17701 cttcccatta ttacgattat tatcaaagga agatagacga tgcgccctat tctcaatatt
    17761 ataattatta taaggggttt atcaattatt tctgggatga attgcctaac ctgaactatg
    17821 ataatcctga agtccgtaac tggatgatga cggtttgtaa atattgggtg gaaaaatttg
    17881 atgttgatgg ctatcgcttt gatgcagtgt ggggtgtaaa tgcacgttta ccagaattta
    17941 cccaaaactt acgattggaa ttaaagcgca tcaagccgga agttatgcta ttggcagaag
    18001 ataaagcttc ctggtcaatg gtttttgatc aacgttttga tgtcgccttt gattgggcgc
    18061 ctactgagga ttgggtttcg cagtggtatt gggaagttga ctattcggaa gacgccacca
    18121 aacaatttac gatttttaat tacagtaatc catcggaaag agttaacctt ttgcaccagg
    18181 cattgactaa ttatgggcaa ggctatgccg ccaacgccaa gattctccgt tatatgggca
    18241 ataatgatat gattcccttt atcaaaaatc ataatttggc tcagactaaa atggtttcaa
    18301 cgctgatttt tgctttgaat gggatgccga tgctttataa tggtcaggaa atcggctaca
    18361 atcaacatcc ttatagtagt taccaaattt ttcaacgagg caaatcaatc cgtagtcagg
    18421 ataaatatgg acttttcgat cattatcgcc atttaattca aatcaggcaa aggtattcag
    18481 cgctccgcag tgattatttt aaagaaatcc cggtagtccc ggcttactat gctttcgcgt
    18541 tccatcgtcg tagtggagat gagaatttgt tcagcgttat caatatgatg agtgagccta
    18601 aaaatattac catctccttg ccgattgagg aattgcatct tgattctacg aaaacctatt
    18661 acctttcaga tttaattacg ggaatgacct tttctgggaa accggaaaat ctaagtagtg
    18721 tggctctgac tattcccggc tatacgacct ctttgttttt attagctgat tcaatggtca
    18781 acgttaatat tgcaccggaa gagcacccag caattgctga aaaattcaca cttttccaaa
    18841 attatcctaa tcccttcaat tcttcaacga cgatcggata tacgattcct caaaagagta
    18901 atgtcaaact caccgttctg gatttgttag ggcgagaggt gatggaattg gtggacagtg
    18961 aacaaaatcc cggtgaacat caggtactaa tccgagccga ccaattggcg agcggttgct
    19021 atttttatcg tttgaaatat gaagaccaaa ccctgatcag gaaaatgttg ttattgaaat
    19081 aagatggaaa gtttagtgtg tgtcagaaaa gcattccaat cgagttttag agctaacttt
    19141 tagtcgtagt tggagtaaat aacattaatc aaataactaa aatttttact atgagcagat
    19201 ggagtttaat tacggtattt ttagcgggat taattatgat tacttcctgc gataaaaatc
    19261 cggttgttga tttaaacggt cggtggcaat tagcgccgga tagcaccggc gaaattacga
    19321 ttaattcgat tgatctgatc gaaaattggc gcatgaccga aatacccggt tcttggcatc
    19381 gcttgcatgt ggacttgtta gattatcagg gagttgtctg gtatcgcaag tcgttcaaat
    19441 taaaatcgct tcctcccaag catcgctttc tgctaaactt cggagcagtg gattaccttg
    19501 ctaaggtata tgtaaaccga gaactggtcg gcgaacacga aggtggttat acgccattta
    19561 gttttgacgt taccgagcaa ttgcaaaagg gagaaaatga aatcctagtg cgagtagttg
    19621 atccggtagc tgatgaattt ggtaccgatg gcattagcta ctggcatata ccgcacggca
    19681 aacaaaactg gtatgtccaa accagcggca tctggcaagc agtccagatg gctatcaaac
    19741 cggaaattta tgtgtcacgt attcaggtta attctcaaat cagcggtgcg tttcaagcaa
    19801 aggtttttat tgagaatcct aagcaggttg ctaatccgaa caaactagtt ttgacaatcc
    19861 gcgacccgaa tggcaaggtc gttttttctg ataaatatga ggggaactct tcgacttccg
    19921 aagcagaatt tacggggcag atcaataatc cccgattgtg gactatcggt caaccaaacc
    19981 tttatactct cgaaatccgt ttgggtgaag atgttctgtt cgatcgtttc ggttttaggg
    20041 aaattgtagt gcgtgatgga aaattttatc ttaatggtaa acctttttat atgatcgccg
    20101 cgctggatca ggatttctat ccggaaaccg tttatgaaac gccgtctgaa gagtatttat
    20161 acgatgaaat gcacaaagcc agagaactgg gattaaatac tcttcggtgt catataaaag
    20221 ttcctgatcc gcgttatctg aaagtggcag atgaattggg tttattagtc tggtatgaaa
    20281 ttcccaactg ggatgtcttt gatgagagcg tcaaagcccg ctcacgtaaa acattgcagg
    20341 aaatgcttca gcgcgattgg aatcacccat cgctggcaat tatcagtctg attaacgaaa
    20401 gctggggact cgaccttaat caagccaatc agcggcaatg gctcaaagct gaatttgact
    20461 ttgctaaatc ccttgccaat ggtcggctaa tagttgataa tagcgcctgc tggggtaact
    20521 atcacctgaa aaccgatatt aatgactacc atacctattg ggcgattccc gaaaatcatc
    20581 agaagtttag agaaacagtc caggaagtta gcgaccgccc acggtggctt tttagtccct
    20641 atggcgatgc ggaagaaacc gggcaggaag ttctgatgat ctcggaattt ggcaattggg
    20701 gtttgcccaa actgccgaaa aaattgccgt tctggttcga ccgccaattt ttagataatc
    20761 tggtaacatt gccggcaggt gttactcaac gatttggaga ttatcaatac ggacgaatgt
    20821 tcaaaaacta taacgaatta gcggaattca gtcagaaagc ccaagcacag gcgttgaagt
    20881 gggaaatcga agaaattcgg ctccaacctg aattgcaagg ttacgtaatc actgaattta
    20941 ccgacattaa ctgggaatgt aacggtttac tggatatgtg gcgcaatttt aaagccgatc
    21001 gccagatact tgcccaagtt caacagcctg acattataat tcctcgacta accaagtatg
    21061 gattttggga taatgaagag atagacgtgc aattatctat ttcacattat agtaatagag
    21121 atttttctaa tgttcgtatc caggggattt acaatggtga gattatcggt gacaagagta
    21181 ttgcgattga ttcggtcggt gtatattttg tcggaagtat gaaaattaat tcagagccgg
    21241 taaaacaacc ggtatctcaa cgtgtcaaat tccgtttatt gagcagggaa ggggatgtct
    21301 tagcggagaa ttttatcgag tttacggtat atccatcgcg aaagatagag aatatttcgc
    21361 cgaattctaa tcttttgata tgtaattcta ttgatctaaa aacaatggat catttgaaaa
    21421 atggcttgga tgtaatatgc gtcattgatt ctagctcagt tttaccggct gattttcctt
    21481 ttacaattac gtcacgggaa tcggatgggt atgacggcaa ttgggcttcg aatttgaatt
    21541 gggttgacga tagtcacagg ccatttaata atttaaaaag taataaagtg ctgggctttg
    21601 aagcctataa ggcattacct aaatatgcca tcaccgggat tcaaccggag cagtttgttg
    21661 atgtattagc cggaatgtat gtcggctggc tccatttgaa ttccgcttat ttaatgcaaa
    21721 tgcgggttgg ctcagggaaa ttgatcctaa cgacctttaa tctaattcag aattatggag
    21781 atgatccttt tacgacgaca ttaagagatg agttagttgc ttatcttcaa agcgaggatt
    21841 gtcaaccaac gataaattgg aaataataat tatttagagg tgaatttatg aatgaaatta
    21901 tttcaaaatt agaaaagcta attgaacata atgacggtat tttgcgtctt aaaccggctt
    21961 gggttgctag ggatttttta ccgccgggta ggcgcttggg attgccggag gaaatgtatg
    22021 atctcggtga gcgcggcggt atttgcgaac gctgggtagg ttcgactaca ccggctgata
    22081 atcgtattaa ggtacccaat gaaggattat gctgtttgga tcttgaagat ggtcaggaaa
    22141 ttactctaaa agcggcagtt gagatagccc cggcattgat tatgggagct gattatgcta
    22201 aggctcatac cgggctggga cgcttgccga aaattttcga ctatgcttat cgcttgcctt
    22261 atcatttaca ccagatggca aaagatgctc gcaaagtcgg tcgcaatagt aaggaagaag
    22321 cctattattt ccccgaagag gtaccgctgg gacctgagcc ggaaacctat tttggtgttc
    22381 atccttatat tagtctggaa aagcgctatg aattactttt accttctatg caggagtgga
    22441 aagatgatag cattcttctc cattcccgtg cctaccgttt aaaggcaggc gatggatttc
    22501 atgttccggc gggaattcta cacgcacctg gctcggcctt gactattgaa ttacaggaag
    22561 attccgatgt gttcgcaatg atgcaggcta aagtaggtgg cgatattatt attgataaag
    22621 aattactatt taaggatgtt tcagaaaaag aacggaaaaa tttggcggaa aaagctattc
    22681 tgggaatgat tgattgggaa ttaaatggcg acccttactt ttatgaaaac cggcatacac
    22741 caccaatact cattgattct gaccagcaat ctggaagtga acagtcctgg attttttata
    22801 atactcataa gtttagcggc aagaagctga ttatccgtcc ggggggaaaa cacgtttcag
    22861 ttgagaaagg agtgtataat attttggctt ggcggggtca gggaacttac ggaaatgtcg
    22921 aaatagaggg tcttaatttt gaaaaagatg aactgttgat ttgccatcag cgggctattt
    22981 acccgatcga agtcgtcaat accggtgacc gcgatttaat tatctataaa tttttcgggc
    23041 cggatataaa cccggatgtt ccgatgttaa ctaaataccc aaaaagtcct aatgcctgta
    23101 atatcctata aaaatgaacc gtttccggat tggtcggaag tcaaaagctg taaaataatc
    23161 ggtttgtcta aaaggcaaaa actacagctc ccatcagagt tttccgaaac attgctgttc
    23221 gttttggatg gtgtggctga aattagcgaa ggagccgaaa aaaggacgat attttcaggg
    23281 gatgttttgg atcaattttt tggtgaggtg tggattgaac ccaaaccgct ggtagaattg
    23341 tttcaaatta gtggcaattg gagtaacgat cgggggagtt gtggggtctt ccggttgaga
    23401 aaaagtgagc atccgcgtaa cgacggcgat ccggtcgagt atgaacgaaa caccgtattt
    23461 gataatcatt tccacgattg tgatgaatac tggttcatta tcaaaggtaa aggtatggta
    23521 gtttcggaag gggaaagcta tgctgttcaa gccggtgatt gcgtaattac cccagccggt
    23581 gaacatcacg actttccaat tgtccaagaa cccattctgg ctgtctggtt tgagggtagt
    23641 atgaaaggca aaaaacgtcc ggggcatttg tatcgtgaaa aatagaaaaa ttaaaagatt
    23701 gtctatcgtc ggttttgttt ttttgattgt tatggctaat aattgttcaa agccgaaacc
    23761 gagagatgcc aatagcatta cattgacctt ttggcatagc ttcgtggcta ctactttacc
    23821 gatattggag gcgatgattg ccgactttga agaagaaaat ccaggtgtca aaattcaagc
    23881 ccagtatgtt ccgaccggag atgcgctggt gcaaaaactt attacggcga ttcaaagcca
    23941 aacggcacct gatattgcct gggttcattc cgattttctg gataatttgg ttaatagtcg
    24001 agcaattttc tcgataaatg aactcgctgg tgacgattca ttattttaca accagtgtct
    24061 tcctgatata tttccgcagc tattaccgcc gataaccttt cgggatactt tatttgctct
    24121 gcctatggaa gccacgacgt tagccctttt gtacaatcgc gattggtttc gacagatcgg
    24181 tttaaatccc gatcatcccc ccgcgacatg ggcggaactg gacagtttta caatgcaact
    24241 gtgtagcgat tctaatagtg atggacagcc ggagcgctac ggattttatg ttcctgtttt
    24301 tccggcttct ggtccgctca atatctggat gacgatgcag tgggcgcctt ttgtttggca
    24361 agcgggtggc gagattttta gtaataatca accaacattt gccagcgagg caggcgtggc
    24421 ggcgcttgcc ttctggagca aaatttatca tcagcaaaaa ttcgagcgct tttcgatggc
    24481 acacgatatg ggttttatta gtcagaccgt agcgatgatt atggatggtc cttggaacct
    24541 cccgcgctat cgggactatg tcaaattcga ctggggtgtg gcaccattac cggcaggttc
    24601 agcaggacga gccagttatt tagctggaga aagtttggtg gtatttaagc aatgtgtcaa
    24661 tccggaagcg gcttggcgtt ttatcaattg gtttctcaag ccggaagttc aacgaacttt
    24721 tgctgaaaaa tctggctatc tgccagttcg acgttcagta ttgaatgatt ctgattatgt
    24781 taattttttg gaacaatttc ctcagcaaaa atctttcaat gaacagattc ggtatgctcg
    24841 aggacgtagt ttgccggata aatttcgggt tgaagtcaat caatgtatcg ccgaagcgct
    24901 ggaaagtagt atcattggta gaatagctcc agaaaaatcg ctaaaagtgg ctgatgagaa
    24961 aatcagacgc ctattatcat cggaaacgga gtaaaaggaa aggctgatga atctgaaagt
    25021 tactttatag aaattaactt aaaatcggga gaaaaatatg agcaatattc taatactgta
    25081 tgattctaaa actggtaata cggctaagat ggctgagttg gttgccctcg gtgccagggc
    25141 aaatggtgat cacgatgtta agattttacg tattgatgat gctaaggcgg aagatattcg
    25201 ttggtgtgat ggcattgccc tcggatcgcc gactaatatg ggaatagtat cttggaaaat
    25261 gaaaaaattt tgggatgact taggtgatga actctgggga aaaattgaag gcaaaatcgg
    25321 ttgcgcattt tcgtcatctg gaggttgggg tggcggcgcc gagattacct gccaatcggc
    25381 tttgatgatt ctcatcaatt tcggattcct agttttcggc gttaccgact acgttgatga
    25441 acagttgaca cttcattatg gagtagctct ggccggtgaa ccgcgttcag ccgcggagaa
    25501 agccgcctgc ttgcgcttag gtgaacgtct ggctgagtat ttatcgaaat ttaaattaca
    25561 atcggacaag aatattaacg aagtgtaaaa ttttacttga aaatgtaatc gggattaata
    25621 gtcaaaggaa cattgtgaaa tattgtgtag taaatttgaa cgttagttaa tttataaaat
    25681 gagaaaggga aaacgttggc ttctatagaa ttaaaacaaa ttgtaaagga atttgataaa
    25741 ggtgctccaa tcattaaaaa tctcgatttg acgattaatg atggtgagtt tttagtgatt
    25801 gtcggacctt ccggatgtgg caaatctacc actttgcgct tgatcgccgg tctggaagaa
    25861 gtaaccagag gtgaagttta tattgacaat cgtctggtta ataatattct ccccaaagat
    25921 cgcgatattg cgatggtttt ccagaactat gcgctttacc ctcatatgac ggtttacgag
    25981 aatatggcgt tcggcttgaa actgcgtaaa tatcctaaat cggaaatccg acagcgggtt
    26041 aaggaagcgg ctgatatttt ggagttaggc gatttattaa atcggaaacc gaaacaactt
    26101 tcgggcggtc aacgtcagcg agtagctctc ggtcgggcaa ttgtccggaa accaaaagtc
    26161 tttttattcg atgagccact ctcgaatctg gatgcaaaat tgcgagttca aatgcgcacc
    26221 gagataaaaa aattgcatca tcatttaaaa acgactatgg tctatgtaac ccatgatcag
    26281 gtggaagctc tgactatggg cgatcgaatt gtcgtgatga aggatggaga aattcatcag
    26341 atcggttcac cttcggaaat ttaccagaat ccgcaagatt tattcgttgc cggcttcatc
    26401 ggtagtcccg caatgaattt cattccaggt gttttacatc agggagtttt ccagagtaac
    26461 accaataata tcaaacttat cttggagaaa tctccttata aggaaatgga taatgtagcg
    26521 gtagtattgg gcattagacc ggaggatgtc cacttgaata agcaagatgc tcactatcag
    26581 gaagtgcggt taactcttga agttatcgaa ccgctgggga atgaatcaat tttgtatttc
    26641 tccgacgagg ggcatcaaat tactcttcgc actcaggaaa ccaatatgcc tctatcaatc
    26701 ggagcatcca tacctgtttt tttcaacata gataaagttc atatattcga tcaaataaca
    26761 gaaaaagcga ttcgataaac gaaaaatcgt gtctatcaat aatctgaagt cacgtgcaga
    26821 aaatttataa ataaggtatt ataaggctct cgtaagacta tcagctatag ctaatcttga
    26881 acgagttgtt gtaagattta gcccacaccg cggagcggtt gaagtattgt agtaagacga
    26941 aaccgcataa aagttattcc tcggagagga tcgagaagaa aatatttgcc gaacgcaatc
    27001 tttacttgac caagaataac tattgcgtta tgcgcttggc taatatttca acaagatttt
    27061 atctgccaga ttatcagaac ttctccctaa ttatatagaa agttaaacct ctctgaggta
    27121 gcaatttctg gcgattatgt ttttctacaa tacttgaagc cctacgggct agttggaaat
    27181 ctatggtttt cagataaaca attaccgcgg a
//
LOCUS       DAOMJU010000022        26011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1721_length_26011_cov_24.130490, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000022 DAOMJU010000000
VERSION     DAOMJU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 26011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26011
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_26011_cov_24.130490"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..818)
                     /locus_tag="PLO58_03525"
     CDS             complement(162..818)
                     /locus_tag="PLO58_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HQO73893.1"
                     /translation="MRTKLTSSIMLILMGIAIMVNVGITQETSVIKLQPPQLDRGKPL
                     MQALNERQSNRQFSAKPLDLQTLSNLLWAADGVNRPESGKRTAPSAMNQQEIDIYVVM
                     KEGIYRYDPKEHALHLIAKGDLRNLCGRQEFVAQAPVNLIYVADYRKMARLNDEERKF
                     FSATDAGHISQNVYLFCASEGLACVVRGMVDRDALAKAMQLSPEQHIILAHTLGYPPD
                     "
     gene            complement(839..2518)
                     /locus_tag="PLO58_03530"
     CDS             complement(839..2518)
                     /locus_tag="PLO58_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HQO73894.1"
                     /translation="MRRFSVSRTWRHLKRYNQVLSVLIKYGFSDIVDAARKDLIARFG
                     EKFIPRGRRNQFSGLSHAERIRRALEELGPTFVKIGQVLSLRPDIIPAEIAAELAKLQ
                     DEVSPLPYDVIANVIQTELGSEPDTVFQDIQSEPLAAASLAQVHKGVLISGEPVAIKV
                     QRPEAESLINTDIEILYDLASIIQNYFLENFALDILAFLHEFDRSIHQELDFVLEGRN
                     LRRFRKFFAGDEAIFIPNYFAEYSTSKLLVMELVEGIKATEIEQLEAAGIDLQVVAQR
                     GVEASFRQVFEFGFFHADPHPGNIMILPGNIIAPIDFGMVGQIDEDTIDNLGHVVVGV
                     LHKDITRTLRSLEDLTGADLSAVAHDLRYDLMTYINNYSELPLKDLQLSLLMDDFFEL
                     VRKYHLRIPSHLALMLKALVTVEGLARILWPNFDVLEALKPYVRKITLRRYNPRRIMR
                     NGTLVLDDFADLMTDLPGRLQAIFKKAAEGNFAIQFQHRNLEHLIDEFRKAANQLSLA
                     LILAALVVGSSLVILTMAGPKLFGYSLIGLLGYVAALLIGLRMLWNHFRKK"
     gene            complement(2527..2820)
                     /locus_tag="PLO58_03535"
     CDS             complement(2527..2820)
                     /locus_tag="PLO58_03535"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73895.1"
                     /translation="MSELIKKILLASVGAVSLTKEKAENLLEDLVKRGEIAREDKTSL
                     LNEILKSIEQSQQETRKFIQKEMEKALKALDIPTRSEFEELKKQVKSRQAHKD"
     gene            2926..5133
                     /locus_tag="PLO58_03540"
     CDS             2926..5133
                     /locus_tag="PLO58_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQO73896.1"
                     /translation="MIQNHEKHFPKKFLGNIATLIIGLVVLVAAVVFGFFSQPKEFGN
                     VDIVLDCLSQDVEILTGVDGIAHISAGNDADLLRTIGYLQATRQLPNLDRLLRLATGQ
                     MSAAHGKKFIELDIAAHQLDFVALAEQTLAELSPEAQTLFEAYCAGINAFIATQRHSV
                     AQQFRLAGYEPHKWEPAECLAILQLFKWAYLSQWNEKIVIYKTGEVFGEERTRDGFPL
                     IEPSTVRESGYQNAFFASLNQLYRDGVNLRRTMNLFPNKDKSLAWILAGKNVPADQTR
                     LIFENNWFNSYDFIFDMAVLDRRMVGLFFPGVPICLAGCNQQVAFGTNWLPENEVNLL
                     VVRINPASEQYLAQDGWHNLRKRSTQIAVRSDHKQEVTFYHTDQGVILDYPIESVDTV
                     AYAIVLKWQAVTASEINQRVALMLIGNNDAARVMTEVSSNLPGLVYLDQKGNYGSSAL
                     PKPLTPNLSTNCLTPFTIDDWRFSSQFTIDSLKSHFLPDWQNQPGTLFAESTDEIFPL
                     LTADKYFTRLMKDVRAALPDTIFTRKTELQAYQNLIKWDGRYAKTAVGPTIYQAFLKA
                     LMRNIYSDELNLVDSETFEQFANSGDFAMQNLLLLIEKGESNWFDNLRTSDFVEWQGE
                     IIRQSFKEAVQLIEEKYSPNLSEWQWGRVNQDFVWGNNIKGKKFSNSKSVSLNSSGVV
                     QTIILKPAGSETTCCIAVNGTTNPVEPVNLLTDRAELLRNGAYIMTLKASPKK"
     gene            5144..6160
                     /locus_tag="PLO58_03545"
     CDS             5144..6160
                     /locus_tag="PLO58_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HQO73897.1"
                     /translation="MLEVIKSTIMITGFVFTMMLVIEYLNVQTRGKWHKLLKGRKWRQ
                     YVLGALLGIIPGCLGSFAMVTLYTHRLVSIGAVVTTMIATSGDEAYVMFALFPGKALL
                     LNVILAIISIPVGVLADLLFRKKYDVVTAHDLPLHDEYCECFQRGKILEQLRSITLER
                     FLLILIMAAILLAAASGFIAGEEEGWVRITLIITSIIGLAIVATVPDHFLTEHLWEHV
                     TKRHVPRIFFWTLGALIVIQILTNYLNIENLLQLQQARVLVLLVACLVGIIPESGPHL
                     IFVTMFAEGLVPFSILLASSIVQDGHGMLPMLAHSRRDFVRIKAVNFGFGLLVGLIGL
                     WLGF"
     gene            6191..6838
                     /locus_tag="PLO58_03550"
     CDS             6191..6838
                     /locus_tag="PLO58_03550"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HQO73898.1"
                     /translation="MNKAFTFLDLTHTLENGMPTFPGDRPKPRIITRSKTQFTISEIH
                     LGTHYGTHIDAPRHFLPDGKSIADFNVDRFTGAALGLHRDSAGTSALYLDDEEIELIR
                     NFKPQWLILRTGFDRFWSQPSYFLEHPYLSVELAQQLIDLKIKGIGGDFPSMDAANAA
                     RQNFPIHHLLMEHEVLIIENLTNLSALPTQELFHLIALPLKIATDGAPARVVAWF"
     gene            complement(6997..7551)
                     /locus_tag="PLO58_03555"
     CDS             complement(6997..7551)
                     /locus_tag="PLO58_03555"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HQO73899.1"
                     /translation="MGQIKSAPQGHLFLAIMSASEHQRQVALEEVRTRFGEIMGAGPL
                     FSVSDFTNYYESEFGRDLKKQLFIFKQPTSLESAHQIKVWTNQIESQFVDNTGSQSHR
                     QVNLDPGYLEPSKLVLFSTKNFSHRIYCGDGVFAEVTLIYEHGQFKILPWTYPDYYWT
                     KNLEFLIQMRNEIVRLSRKDSKQA"
     gene            complement(7538..8926)
                     /locus_tag="PLO58_03560"
     CDS             complement(7538..8926)
                     /locus_tag="PLO58_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQO73900.1"
                     /translation="MSDRSLLLINPWIADFTAFDLWSKPLGLLYIAKFLQNYGYEIEL
                     LDLMDRQRWQDTSNDKFNDGRGKYRKTIIAKPPVLARYPRRFGLYGASREQFSDTLSN
                     MARPNAILVTSLIAYWYPGVAATVEILRRYFPETPLILGGIYASLYPEHARNSIQPDF
                     LITGYGEKKTLQLVDQLFGIERDYNVIPDYNDNGILPWRLYQKLKSVAVLTSRGCPNR
                     CTYCATYLLNKKFVQRRPEDVISEIISTYNQFNVENFAFYDDALFAHRDRHIIPILKG
                     IISAGVKINLHSPNGLFAREINSELAELMFSAGFKTVRLSLESTSSQIQKSSSNKVNN
                     ANYTDAVNNLAKAGFARNSLETYLIMGLPGQTRQQIIDSINFVADQGVIVKLASFSPI
                     PGTTEWERARALGYVSDDQDPLMTDATIFPFLSRNLSYEDYLALRQVANERNTENRSN
                     NQISNEASDGTD"
     gene            complement(9126..10031)
                     /locus_tag="PLO58_03565"
     CDS             complement(9126..10031)
                     /locus_tag="PLO58_03565"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO73901.1"
                     /translation="MRQKLRTGFIIIITGMFLQAESGESGMAFLKIPVSAQTASTMGV
                     FAQVSGTPFTIFDNPIGIINDDIKIGFSHNFWFADVSGDALAATLPLKKGNLGFGLNF
                     VKIPGIEIRDIPSDESLGEVEAQYMSAAVGYAYPIFSKSSIGCTVKYLYEHLYAENAG
                     GIAFDVATHWHIPSGMDLALSLNNIGQMQTLKDKATTLPTLFKIGIVRPEIFADSPFN
                     VNLGINLIANLVTEKTYFQVGGEVAFTNLIKIRGGYERVGTINRTALGAGLNFGRFEI
                     DYAFIFMPDGLGYPNLLTLSYRPEL"
     gene            complement(10040..10966)
                     /locus_tag="PLO58_03570"
     CDS             complement(10040..10966)
                     /locus_tag="PLO58_03570"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO73902.1"
                     /translation="MRKLLNLILSLIFSMALLQGETNDNAGYAGAFLRMGLGARATAM
                     GNSGVAFPSNGFGIYYNPAGLAYMDERNLALSYSFLSLDRQFHFIGLSVPLPPNAGVG
                     VSWLHAGVKDIEGRNSIGQVDETYQTGENVVLISFANAFHPRISLGINFKILHNQMLD
                     VSATGLGFDFGVLYQPLEWLTAGLQFKDIGAGYNWNTQDLFGEEGSSYKENFPQILKF
                     GLAIKPIQELTFVGDIGVSDQNVWQTHCGAEYVYRNLAFLRIGMNQLSPTFGAGLAYG
                     FIANMTTQLDYSFIIGAVGEGTTHIFSWQFQF"
     gene            complement(10969..12540)
                     /locus_tag="PLO58_03575"
     CDS             complement(10969..12540)
                     /locus_tag="PLO58_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HQO73903.1"
                     /translation="MKKYPAILFIIGFLFTSGFSQAFRLAKPTLDSDNTFLGLASNSV
                     TEIVSQGNSLMWFATGGGLSMSPDFGQTFVSFYPGLNNLPRGGISAIAVKGDTIWVAA
                     VFDSLIQSYGYQQTGGGLAFSIDKGKNWTFVPQPIDDPADNSAEWNNQSVSFLPVTTP
                     INNTTWDIAITDDYVYIVSWAGGIRRSADMGQTWQRIPLPSDDLNNLDCDDLIDFEIN
                     PRDPPDGNHNHKGFSILAYGDTIWIGTADGINLGIVESPECISWRKFNAQNSAISGNF
                     VVAIDRQLWKGKETIWAATLTVGESGEYRAVSKTSDGGLTWTTTLVNERPYNFAFQDS
                     IVYVCTERGLFKSIDGENWAIYTPIADIASGEQILTQYFYAAEVDRREGPTYLWVGTG
                     DGVAKTASDGLTWTVYHSAVSTKSPGQPAIYAYPNPFAPRYQNVIGDDGHVRIQYNID
                     QPASVRLEIYNFAMEQVYRSDWKTVMDAGDHHLIWNGRATNGEIVANGTYFCKLIQKS
                     NGNEVEHWTKLIVIN"
     gene            complement(12537..13604)
                     /locus_tag="PLO58_03580"
     CDS             complement(12537..13604)
                     /locus_tag="PLO58_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73904.1"
                     /translation="MKRIIIAALFITILTLWTCENKSIVETVEPTPIIENLTLHYSEL
                     NHSLFIAAKVEDAQGWDNLDSVVFNLYRINTIDSMNGFLFKSGKLSDDGPPRDIITQD
                     GVFSYLIDSTELGSYEGYYRVFVQAYDADGNVSDSATATELVQANSPPEIFLLSAPTT
                     FEKWDTLKFYFSVTDKQGYDDIVGVYYSILRPDGQFFDPYFYLNDAGEYGDEKARDGI
                     FTVFQPSNPESDLQGLYTFYFFAEDYSGAMSDTITLKIASPGIHITAPASALTLRFGQ
                     TLRIMWEYAYLSQVKIEYTFNANETNPDWIVADTTAAALKYYDWTVPATQVTNYCKVR
                     VSDLTKPARYDVNDDYLVITQ"
     gene            complement(13605..14429)
                     /locus_tag="PLO58_03585"
     CDS             complement(13605..14429)
                     /locus_tag="PLO58_03585"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQO73905.1"
                     /translation="MTANSERWLKVLVPKNAGNEELIIARLIQWGMLGLQETDTDYEI
                     YFPLNMKLSLPELIAAEWPQLNITITEIVDNGWSTRWQEYFKPQTISPRIAVRPYWEK
                     PLKDVSVEIVLKPGMAFGTGTHPTTHLALLMLEKYLTPGDVLLDAGCGSGILTIAALK
                     LGAAQVSAWDIDSLVIDNFNENLELNGLSRKVAIQIGDVTALPNYNYDLIVSNIERQS
                     NLRLLEALVRQQNRPLVIFTGLLKEENEIFTNKVLNYGREILDQDTEDEWLALVIK"
     gene            complement(14435..15193)
                     /locus_tag="PLO58_03590"
     CDS             complement(14435..15193)
                     /locus_tag="PLO58_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQO73906.1"
                     /translation="MLIEFLRPLADTLIIQNFEYLIPEENEVLNLTNIIAGFNIRSAN
                     GLLIGAHWDTRPRAEYDPDPAKRKTPIIGANDGASGVAVLMHLAEILSRQQPPRTIYL
                     VFFDGEDYGFPGTLDYYCLGSEYFAKNLPIPKPEEAIIIDMIGDADLRIPVERNSVKS
                     HPQLIKKLWAIADRHNFSAFVHQIDEEIYDDHLMLIQHAGIPSVDLIDFQYPNRFINY
                     WHTTLDTPDKCSAESLEIVGTVLYDYIFHGSEER"
     gene            complement(15352..>16439)
                     /locus_tag="PLO58_03595"
     CDS             complement(15352..>16439)
                     /locus_tag="PLO58_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73907.1"
                     /translation="KRPRKPGSTIDKGEDLKPTSGQTLEQMPKPVDDVKKPEVNITQI
                     VAKYLKKIQPFNFNLSLNNSITNQYRTGTPDWQYRMGLTRDPGIPFVMEEYIPTSAEN
                     TSNSFDFSVRSGINLFSNVVIPLSFAQNVSNTQSQGTVSRTLTRDYIPSGINGKDGFP
                     FPSWSLTWSQLEKYKIFSKYFKKLSLDHGFSGKESIITQNGKEMSSSYKRYFQPLIGL
                     SMQFVNDISASARVTSGQSINNASGGTTIIDDAGASISLSWQKKGGLSIPLPFMKDKQ
                     LKNNITFTANFDYTNSTTRAKQGSATKFAVTAKSNSWKTTPRINYTFTDKVTGGLFFE
                     YGESYNRLTGKRVTRNYGFDVNIAIRG"
     assembly_gap    16440..16539
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16540..21469)
                     /gene="sprA"
                     /locus_tag="PLO58_03600"
     CDS             complement(<16540..21469)
                     /gene="sprA"
                     /locus_tag="PLO58_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HQO73908.1"
                     /translation="MAADREPDVFPLKIDWLSNRYTPANIESKRGLSLISPDYATLNQ
                     NILKVQFPGMKVTNTLDKSGQHFIFYNDLNGAPVLTPIGIPVASYFDLRQRLNWHNKL
                     VEVVTAKPADTKAASDKLELIGADIAGQHVALRVSGNININGRLQTQKRSQVSSSYAE
                     GTSTSFIVDQKQQLNIEGKIGDRISILVDQDSERDFDFENAMKIIYTGKEDEIVQKVE
                     AGNVALSLPGTQFVTFSGKNNGLFGLKALMKFGGVDVTAIASVEKGKKEKLSIDGGAQ
                     TSNISIKEYEYRRNLYFFLDTLFRDNMYRGYQFDQLFHADPRGKVTDLQVYKSQTIET
                     AGCAYGTAYVDPRDTTLYKDYSENRIFKLLELNKDYYVDVTLGYIRMTTQVQDGEILA
                     VAYRLTDDNNVTVTGEEYGDYNRSDTSTAHIILKLIKPQEMLPTHPCWNLEFKNIYYL
                     GTTGINSDGFELDIVYTLGEGEQNYDLVTGKKFLEMFGLDRVDNNGNPTPDGVIDMNN
                     GAILNLYSGELWFPYLRPFQYDSTDNAGKRNPNISKIYNCSAMYDSNRTDNDILSETK
                     FKIMVSYKNRSSILNLGAMVIENSETVTLGNSTLERGKDYIIDYFSGTLTLLTSDATD
                     PNAKLEVKYEKNQFFQLDKKTILGARAQYDFGENSYIGATALYFGQSVSDEKVEVGYE
                     PMHNFVWDINGRYNRNLDFLTRAVNWLPLVSTNAASSFDIEGEVAQVLPNPNTLNNEE
                     TGDRHGVGFIDDFESSKRVTSPPIMRRYWSNAACPLGKDENERGFTFWFNPYGGVPTK
                     SIWPDKEVSTTAQNNITEIMVMSVDPNWSIAVVDGVSPVHSAWGGITYAFPQSYYDQS
                     TTKYVDIWVKGNSGTLHIDLGQISEDQIPNKKLDTEDIPDAGLTMGNGLLDPGEDTGI
                     DGLADKNEKIVTTKYGELSYGDTRLTEYKRDISDPHSDNWKYTEGSKEYRYINGTENN
                     EKDANGLFPNSEDLNNNYAIDLTNDYFTAAFDLSDPNNEYIAGKTYIDSRPTGWKLYQ
                     IPLTAFSKSATGKDISWESIRACRIWFDGLTGEDSVFIAKVELVGNEWEDLGIAKDDT
                     SIFVVNENSFSISVINTEDNPDIYEPPAGVQGEYDEINQIRTKEQALVLNLPEEGSGL
                     FPGEFCAAKKVLAEETSYITYKVMKLFVNGHELKADGYQFSEGEETPLQFFIRFGKIF
                     ENSPTTTNYYEYRQPIYPGWAEENEMELDLDFLTSLKNYESADDYIGDGEYRIYTNTS
                     GVTIRHYKEKFNDDYTGREIIIYGKPSLANVKHLNIGLRNRADNSGYVNKFKTGTIKG
                     QVWLDELRLSEVRKDKGIAYRAKASLRVADLASFDVSVNYRDADFHTVEQRPSLQTEN
                     LKTTEALTATGRISLDKFTPASWGLRLPVSGSYTQTTGTRKYIYGSDILMKENAPDSL
                     LDISHSYGVNTSIEKRASDFWLTKYTIDQIKISANATWTDASSVTVRESQSESYKASI
                     SYNFPFPKDKYINIFKWTAPVPLIGNKLSEIRFYYFPNALTVTADAGRSESYSTPRYG
                     TTITSSNSFGLNRNLSLGYKIFDNLSANYTLNLENDLIELWQNQWEILKSANPGQITN
                     MRESYSVNFNPKIAKWFNPTFNYTSNFNWREPI"
     gene            complement(21625..22233)
                     /gene="rdgB"
                     /locus_tag="PLO58_03605"
     CDS             complement(21625..22233)
                     /gene="rdgB"
                     /locus_tag="PLO58_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQO73909.1"
                     /translation="MDILIATHNRDKVREIKRIFSLPELIWHTLDEYPDCPKVVEDGN
                     TLFENAFKKARMIAEFTGLPTIADDTGLEVDALHGQPGVFSARFAGENATYAQNVAKL
                     LEVMRDIPDSERQAHFRCVAVYYSPDLTLVEEGKIDGIILRQPRGNGGFGYDPVFYLP
                     QLGRTFAEMDLSEKNSLSHRGQAFTRLANKLKSVDKFVTDNI"
     gene            complement(22498..23070)
                     /locus_tag="PLO58_03610"
     CDS             complement(22498..23070)
                     /locus_tag="PLO58_03610"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQO73910.1"
                     /translation="MTKIIRCPWPANDELMIHYHDTEWGVPLHNDQKLFEFIVLDSFQ
                     AGLSWRTILHKRENFRRALAGFEVNQVANFGEIEINSLLANAGIIRNRAKIQATINNA
                     ARFLEVQLEFGSFDRYIWQFTAGKTIVNHWQKLSELPDKSLEAEAMSADLIRRGFRFV
                     GPKICYAFMQAAGMVNDHLESCFRWQELQD"
     gene            23419..24288
                     /locus_tag="PLO58_03615"
     CDS             23419..24288
                     /locus_tag="PLO58_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQO73911.1"
                     /translation="MDIVALARKKGLLFDGAFGSMLIQHGLPTNANAEVWCLEHPEVV
                     TKIHAEYVAAGADVLTTNTFGASSLKLQKAGVEKRVAEINHRMVEMARAAAKPNTLIA
                     GNIGPTGEMLAPFGKISIEEATINYAEQAGFLDKAGVDLFIIETMFDLNEALAAIKGI
                     LQVSAKPIFATMTFSESPTGFVTIMGNPVEESMRQLVEAGATAVGANCSIGSEKMVSL
                     AQIIRAAVSVPVVIQPNAGIPILRNGILHYPEDVAFYTRNIMTIKQAGVEIVGGCCGT
                     TPEYIRAIRQALT"
     assembly_gap    24330..24429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24430..25007
                     /locus_tag="PLO58_03620"
     CDS             <24430..25007
                     /locus_tag="PLO58_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQO73912.1"
                     /translation="NFTQTLECQYCRETRELLEEITALSDKLSLEVYNFQIDRSVAER
                     YGVDKIPATILFAETAPHSRIKFYGIPAGYEFAALLEDIVDISNNKTDLSPEIVTDLK
                     KVTWPVHIQVFSTPTCPYCPAAVRTAHRFALLNENIRADMIEAIEFPHLAQKYNVRGV
                     PRSVINENIIIDGAVSEKQFLEKILSSQPTT"
     gene            25065..25655
                     /locus_tag="PLO58_03625"
     CDS             25065..25655
                     /locus_tag="PLO58_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQO73913.1"
                     /translation="MENNLPEEELVARAKKGDQAALSQLVLNYSSRIYHLGLRLTGSH
                     QDAEDILQDTFLIMVKKINQFQGKSSFYTWLYRIAMNVGLRKLKSKPRKYEHISLDDP
                     DFERVSSEAASEWPDFDYDIVNDPHFRQRLDRLIEQLPEKYRTVFILRDLHGLSTEDT
                     SRILQITPSNTKIRLMRARNFLKENLEKMLKTEMQL"
     gene            25652..25903
                     /locus_tag="PLO58_03630"
     CDS             25652..25903
                     /locus_tag="PLO58_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73914.1"
                     /translation="MKLDEELLKEICEYMGSDLNSAPCKIIRDHMEACPNCEVYIDKI
                     KRTIEIYRLADQCEEIPEKVTRNLLACLKLDDPSKTCKK"
BASE COUNT         7262 a         5676 c         5513 g         7360 t
ORIGIN      
        1 aatcaagaaa ttataccagt tttaacgtag tcgtgacttt ttcaggaaac cctaaatata
       61 ctgggggccg agtgggcact agaaggcgaa cagatacgat gagtccctgc tcgccttcgc
      121 tttttaaaag tcggaaattt ttagtaattc aaaattcgca tttaatctgg aggataacct
      181 agagtatgtg ccagaataat atgctgttcc gggctcaatt gcatggcttt agccaaagca
      241 tcccggtcaa ccattccacg gacaacacaa gccaacccct cagatgcaca aaataaatag
      301 acattctgac tgatatgacc ggcatcagtc gcagagaaaa attttcgctc ctcatcattt
      361 agtcgagcca ttttacgata gtccgccacg taaattaaat tgacaggggc ttgggcaacg
      421 aattcctgcc gaccacaaag gtttcgcaga tcacccttag ctatcaaatg aagagcgtgc
      481 tccttaggat cgtaccgata aatgccctcc ttcataacca catagatatc gatttcctgt
      541 tgattcatag cactaggtgc ggtgcgtttg ccggattcag gtcggttaac tccgtctgcc
      601 gcccagagta aatttgacaa agtctgaaga tcaagtggtt tcgcactgaa ttggcggttg
      661 ctctggcgct cattcagtgc ttgcattagc ggtttgccgc gatctaattg cggtggttga
      721 agtttgatga cggaagtttc ttgagtaata cctacattaa ccataattgc tatccccatt
      781 aaaattagca taattgagct agtgagtttc gttctcattt ttttgttcta ctcctatttt
      841 atttttttct aaaatgattc caaagcattc tcagacctat taacaaggcg gctacataac
      901 cgagtaagcc aattaacgag tacccgaata atttgggtcc tgccattgtc agaatgacta
      961 acgaggaacc tacgaccaat gccgccagaa ttaatgccag cgaaagttgg ttggcggctt
     1021 tccggaactc atcaattagg tgttcaagat tgcggtgttg aaattgaatg gcaaagttac
     1081 cttcggcggc ttttttgaaa attgcctgta gtctgccagg cagatcggtc atcaaatcgg
     1141 caaagtcgtc taatacgaga gttccgtttc gcataatccg gcgcggatta taacggcgca
     1201 gggtaatttt tcttacataa ggtttcaaag cttccaatac gtcgaaattc ggccagagaa
     1261 tacgcgccaa tccttccact gtaaccagag ctttcaacat caaagctaaa tgcgaaggaa
     1321 tgcgcagatg gtatttccgt actaactcaa aaaagtcatc cattagaaga ctcagctgta
     1381 aatcctttaa gggcaattca gaataattat tgatataggt catcaagtca taccgtaaat
     1441 catgggctac tgctgataaa tcagcaccag tcaggtcttc taaggaacgc aaggttcggg
     1501 taatgtcttt atgcagaacg ccgacgacaa catggccgag gttatctatg gtgtcttcat
     1561 caatttgacc aaccataccg aagtctatcg gtgcgataat gttgccaggc aggatcatta
     1621 tattaccagg atgaggatct gcatggaaaa agccaaattc aaatacctgg cgaaaactgg
     1681 cttcgacgcc gcgctgggca actacttgaa gatctatacc agccgcttcc agttgttcga
     1741 tttctgtagc cttaatccct tctaccagtt ccatcactaa tagcttcgat gtactatact
     1801 ccgcgaagta atttggaata aagatagcct catcaccggc aaaaaatttc cgaaaacgcc
     1861 tcagatttcg accttccagt acaaaatcca gttcctggtg aattgaacga tcaaattcgt
     1921 gcaggaaagc caaaatatca agagcaaagt tttccagaaa ataattttgt ataatagatg
     1981 ccaaatcgta taaaatttca atatcagtat tgatgagcga ctcagcctca ggacgctgaa
     2041 ctttaatagc aaccggttca ccgctaatta gaacgccttt atgaacctga gccagggatg
     2101 cggctgctaa cggttctgat tgaatatcct gaaaaacggt atcgggttcg cttcctaatt
     2161 cagtctgaat tacattagca ataacgtcgt aagggagcgg tgaaacttcg tcctgaagct
     2221 tagcaagttc ggcagcaatt tcagccggaa taatatctgg acgcagactt aaaacctgcc
     2281 caatcttgac aaaagtcgga ccgagttctt ctaacgctcg acggattcgc tccgcatggg
     2341 acaggccact gaattggttc ctgcgaccgc gcgggataaa tttttcaccg aaacgtgcaa
     2401 tcagatcttt acgggcggca tccactatat cagaaaaacc gtacttgatt aaaaccgaaa
     2461 ggacctgatt atagcgtttg agatgacgcc aagtgcgcga aacggaaaaa cgcctcatca
     2521 ggggacttaa tctttgtgcg cctgacggga tttaacctgt tttttgagct cttcaaattc
     2581 cgaacgagtc ggaatgtcaa gagctttcag ggctttctcc atttcttttt gaataaattt
     2641 gcgagtttcc tgttgacttt gctcaataga cttcaagatt tcattcagca ggctggtttt
     2701 gtcttcacga gcaatttcac cgcgtttaac caggtcttcc aacaggtttt cagctttctc
     2761 cttggtcaag gaaacagcac caacacttgc cagcaaaatt tttttaatca gctcactcat
     2821 aatgcattct ccttttttac aatattattt taatcaactt caagcgtaaa agcaatttta
     2881 catcttcttg tcacgcccct taaaataaat tatatttacc gtagaatgat acagaatcac
     2941 gagaaacatt ttcctaaaaa atttctgggt aatatcgcga ctttaattat cggattggtt
     3001 gttctggtcg cggcggtcgt tttcggattc ttcagtcaac caaaagaatt tggaaatgtt
     3061 gacatcgttc tcgattgttt gtctcaagac gtggaaattc tcaccggagt ggacggcatt
     3121 gcccatatta gtgccggtaa tgatgctgat cttctgcgaa ctattggcta tctccaagcc
     3181 acccgacaat tgccaaatct ggatcgtctc ttgcgcttag ccactggcca gatgtctgcc
     3241 gcacatggta aaaaatttat cgaattggac atcgccgccc accaacttga ttttgtcgct
     3301 ttagccgaac agacactggc agaactttcg cccgaagcac agacactttt tgaggcttat
     3361 tgcgcaggaa taaatgcttt catcgccacc cagcgccatt cagttgccca acaattccgc
     3421 ctggccggtt atgaaccgca caaatgggaa ccagccgaat gtctcgccat tttacagtta
     3481 tttaaatggg cttatttaag ccaatggaat gaaaaaatcg tgatttacaa aaccggcgaa
     3541 gtgttcgggg aggaacgaac tcgcgacgga tttcccctga ttgagccaag tactgtccgc
     3601 gaatcgggtt accaaaacgc cttctttgcc agccttaatc agctgtatcg ggatggggtt
     3661 aatttgcgcc ggactatgaa ccttttcccg aataaagata aatcgctcgc ctggatattg
     3721 gctggtaaaa atgttcccgc tgaccagact cgactaattt ttgaaaataa ttggtttaac
     3781 tcttacgatt tcattttcga tatggcggtt ctcgaccggc gaatggtcgg tttgtttttt
     3841 ccgggcgtcc cgatttgcct tgccggctgt aatcagcagg tagcatttgg gactaactgg
     3901 ttgcccgaaa atgaggtcaa tttgttggta gttcggatca atccggctag tgaacaatat
     3961 ttagcgcaag acggctggca caatcttcga aagcgtagca cgcagattgc cgtccgcagt
     4021 gaccataagc aggaggttac attttatcat actgatcagg gtgtaattct cgattatcca
     4081 atcgagtcgg ttgatacggt tgcgtacgct atcgtcctga aatggcaggc agttactgcc
     4141 tcagaaataa atcaacgagt agcattaatg ttaattggta ataacgacgc cgcaagagtt
     4201 atgactgaag tcagttccaa tctgccgggt cttgtttatc ttgaccaaaa aggtaactat
     4261 ggttcctccg ctttacccaa accgctgacg cctaatctta gcactaattg cctgacgccc
     4321 ttcacgattg acgattggcg tttttcatct caattcacga tagattcact gaaaagtcat
     4381 tttttaccgg attggcaaaa tcaaccagga acgttatttg ccgaatccac tgacgaaatt
     4441 tttccgcttc tcacggctga taaatatttc acaagattga tgaaagatgt acgcgccgcc
     4501 ctaccagata cgatttttac gcgcaagaca gaactccaag cctatcagaa tctaattaag
     4561 tgggatggtc gctatgctaa aaccgccgtc ggtccaacga tttatcaggc ttttctgaaa
     4621 gcgctaatgc gaaatattta cagcgacgaa ctaaatttag ttgattcgga aacgtttgag
     4681 caatttgcca attccggtga ttttgctatg caaaatttgc tgttactaat cgagaaaggc
     4741 gagagcaatt ggtttgataa tctacgaaca tctgattttg ttgaatggca gggagaaatt
     4801 attcgccaat catttaagga agcagtgcag ttgatcgaag aaaaatactc gccgaattta
     4861 tccgaatggc aatggggacg agtaaatcag gatttcgtat ggggcaacaa cataaagggg
     4921 aaaaaatttt caaatagcaa aagtgttagt ctcaactctt ccggcgtggt ccaaactata
     4981 atcttaaaac ctgccggaag cgaaacgact tgttgcattg cagtcaacgg tacaacgaat
     5041 ccagtagaac cagtcaattt gttgaccgat cgagccgaac tattgcgaaa tggtgcttat
     5101 attatgactt taaaagcgag tccaaaaaag taaggagagt aatttgcttg aggtaatcaa
     5161 atctaccata atgataaccg gcttcgtttt cactatgatg ctggtaatcg aatatttaaa
     5221 cgttcagact cggggaaaat ggcacaaatt actcaaggga cgaaagtggc ggcaatatgt
     5281 tctgggagcg ttgcttggca taattccagg ctgtctcggc tcatttgcga tggtcacgct
     5341 ttacactcac cgcttagtat ccataggcgc cgtagtaaca acgatgatcg caaccagcgg
     5401 cgacgaggct tatgtaatgt ttgccctctt ccccggcaaa gctttgttat tgaatgtaat
     5461 tttagccatc atttcaatcc cggttggcgt gttagccgat cttttatttc gcaaaaaata
     5521 tgacgtagta actgctcatg acttaccgtt acatgatgaa tactgtgagt gctttcagcg
     5581 gggaaagatt ttagaacaat tacgctctat tactctcgaa cgatttctgt taattttaat
     5641 tatggcggcg attttgctag ccgccgcgag cggtttcatc gccggagagg aagagggctg
     5701 ggtgcgaatt acgttaatta ttacttcgat aatcgggctg gcgatcgttg cgacggttcc
     5761 tgaccacttt ctgacggaac atctctggga acacgttact aaacggcatg tgcccagaat
     5821 ttttttctgg acactcggag ctttgatagt gatccagatt ttaaccaatt atttgaatat
     5881 cgaaaactta ttgcaattgc aacaggcacg tgttctggtt ttattagttg cctgtttggt
     5941 tggaataatt cccgaatcgg gaccgcattt aattttcgtg acaatgttcg ccgaagggct
     6001 ggtgccattt agtattttac ttgccagttc gattgtccaa gacggtcacg gtatgttgcc
     6061 gatgctggcg cattcgcggc gtgattttgt tcgcatcaag gctgtcaatt ttgggtttgg
     6121 tctattagtc ggtttgattg ggctgtggct tggtttttaa tccattattc aaaacgtcag
     6181 gaataagaaa ttgaataaag catttacttt ccttgatcta acccataccc tggaaaacgg
     6241 tatgccgacc tttccgggcg accgtcctaa gccgcgaatt attacgcgca gtaagacgca
     6301 atttacgatt tcagaaattc atttaggcac tcattatgga acgcatattg acgctccgcg
     6361 ccattttttg ccagatggaa aaagcatcgc tgattttaat gtagaccgct ttaccggcgc
     6421 ggcgcttggc ttgcatcgtg attcggctgg cacatccgct ctgtatctgg atgacgaaga
     6481 aattgaatta attcgaaact ttaagccgca gtggctgatt ctgcgcactg gatttgaccg
     6541 gttttggagt cagccgagtt attttttaga acatccctat ctatcggttg aactcgccca
     6601 gcaattaatt gatctaaaaa ttaagggtat cggcggcgat ttcccgagta tggatgccgc
     6661 taatgccgcc aggcaaaatt ttccaattca ccatctctta atggaacatg aagtattaat
     6721 tatcgagaat ctgaccaatc tcagcgcgct tccaacgcag gaactttttc acttgattgc
     6781 tctgccgctc aagattgcta ccgatggcgc ccccgcccgg gtagtagcat ggttttagac
     6841 aggactgacc ggatttaggt ttttttagca gaattagcct gacaaaatat ttaaggaaat
     6901 tttttcaatg aaagaagtag ttatcattgg agaaatcctg gagatttaat tggattcctt
     6961 tgttgaacta tagcagaatc ttttataaaa atactgtcaa gcctgtttac tatccttccg
     7021 actcagacgc acgatttcat ttcgcatttg tattagaaat tcgaggtttt tcgtccaata
     7081 ataatcgggg taagtccaag gtagaatttt gaattggcca tgttcgtata ttagcgttac
     7141 ctccgcgaag acaccgtcac cgcaatagat tcgatgcgag aaatttttag tagaaaataa
     7201 aactaatttc gacggttcga ggtagcccgg gtcaagatta acttgccgat gggattgact
     7261 gccggtatta tctacaaatt gggattcgat ttgattggtc cagactttaa tttggtgagc
     7321 actttccagg ctggtaggtt gtttgaaaat gaaaagttgc ttctttaaat cccttccgaa
     7381 ttccgattca taataattgg tgaagtcgct gacggaaaaa agcggaccgg cgcccattat
     7441 ttcaccaaaa cgagtccgca cctcctccag tgcgacttgg cgttgatgct cactcgcaga
     7501 cattattgct aaaaataaat gaccttgcgg tgcggattta atctgtccca tcacttgcct
     7561 cattagaaat ttggttattg cttcgatttt cggtattgcg ttcattggct acttggcgca
     7621 gagctaagta atcttcatag ctgagattgc gggaaaggaa cggaaaaatc gtggcgtcgg
     7681 tcatcagcgg atcttgatca tcgctgacat agccaagcgc acgagcacgc tcccattcgg
     7741 ttgtgccggg aatcggcgaa aaagatgcca attttacaat cacaccctga tcggcgacga
     7801 aattaatact atcaataatc tgctggcgag tttgaccggg taagcccata atcaagtaag
     7861 tttccagtga attgcgcgca aatccggctt tagccaggtt attgacagcg tccgtataat
     7921 tcgcattatt gactttattg ctggaggatt tttggatttg agacgaagta gattccagac
     7981 ttaagcgcac agttttgaaa ccggcagaga acattagttc ggctaattca gaattgatct
     8041 cacgggcaaa aaggccattt ggtgaatgca ggttaatttt tactccggca gaaattatcc
     8101 cctttaatat tggaataatg tggcgatcgc gatgggcgaa aagagcgtca tcgtaaaaag
     8161 cgaaattctc tacgttaaac tggttgtaag tcgaaataat ttcgctaata acatcctcag
     8221 ggcggcgctg aacgaacttt ttatttaaaa ggtaagtcgc acagtaagta cagcgattag
     8281 ggcaaccgcg tgaagtcaga actgccactg actttagctt ttgatataaa cgccagggca
     8341 agatgccgtt atcattgtaa tctggtataa cgttataatc ccgttcaatt ccaaaaagct
     8401 ggtcaaccaa ctgcagagtt ttcttttcac cataaccggt tatcaagaag tcgggctgga
     8461 ttgaatttcg ggcatgctct ggatataaac tagcataaat tccaccaaga atcaaaggcg
     8521 tttcagggaa atagcggcgc aggatttcga ccgtggcggc gaccccggga taccagtagg
     8581 caatcaacga cgttactagg atggcgttcg gtcgcgccat attggagaga gtgtcactga
     8641 actgttcccg actagcgcca taaaggccaa aacgtcgcgg gtagcgcgcc agcaccggtg
     8701 gtttagcaat gatggtttta cgatatttgc cccgtccgtc attgaactta tcattactgg
     8761 tatcctgcca gcgttgacgg tccattaaat cgagtaattc gatttcataa ccgtagtttt
     8821 gtagaaattt ggcaatatac agcaagccca gcggctttga ccacaaatca aaagcggtaa
     8881 aatcggcgat ccagggatta attagtaata aacttctatc agacataatt aaaatctatt
     8941 gaatttccat gctgataaaa agtgtttaat acatccggtc agttctgagg atattttaac
     9001 gagtgaactc actacaagat gaatgcttac ctcgaataga aatagtcaaa aaaaccaaag
     9061 tggagtccgg gttagtaagc tgatatattt tgatagctgg ttggcttatt tcgagaatag
     9121 taagcttata gttctggtcg gtagctaaga gtaaggagat tgggataacc cagcccatcc
     9181 ggcataaaaa taaaggcata atcaatttcg aaccgcccga aatttagacc ggcccccagg
     9241 gcagtccggt tgattgtccc gacccgttcg tagccgcctc gaattttgat taagttcgtg
     9301 aacgccacct ctccgccgac ctgaaagtag gttttttcag ttaccaaatt agcgattaaa
     9361 tttatcccga gattaacatt aaaagggctg tcggcgaaaa tttccggccg tacgattcct
     9421 attttgaaaa gagtcggcaa agtcgtagcc ttatctttca gcgtttgcat ctggccaatg
     9481 ttatttagcg ataatgctaa atccatcccg ctcggaatgt gccagtgagt agcaacgtca
     9541 aatgctatcc caccggcatt ctcggcgtag aggtgctcgt agagatattt cacggtacaa
     9601 ccaatagacg atttcgaaaa aattggataa gcatatccga cggcggctga catatattgc
     9661 gcttcgacct cacctaacga ttcgtcggaa ggaatgtctc gaatttcgat acctggaatc
     9721 ttgacgaaat taagcccgaa tccgagattg ccttttttga gaggaagcgt ggccgccaga
     9781 gcgtcgccgc tgacatcggc aaaccagaaa ttatggctga agccgatctt gatgtcgtca
     9841 tttataattc caatcggatt atcaaaaatg gtaaacggcg tgccgctgac ttgggcaaaa
     9901 acgcccatcg tcgaagcagt ttgagctgaa actgggattt ttaaaaacgc cattcctgat
     9961 tcgccgcttt cggcctgtaa aaacatgccg gtgataatta tgataaatcc tgttcttaat
    10021 ttttgtcgca tagtaaccat caaaattgaa attgccatga aaaaatgtga gtcgtgcctt
    10081 caccaacggc acctattata aaagaataat ctaattgagt agtcatatta gcaataaaac
    10141 catatgccag acccgctcca aaagtcggac ttagttggtt catcccgatc cgcaaaaatg
    10201 ccaggttgcg ataaacatat tcagcaccgc aatgagtttg ccagacattc tgatcggaga
    10261 ctccaatatc tccgacgaag gtcagctcct gtatgggttt aatagccaga ccaaatttga
    10321 gtatctgggg aaaattttct ttataactac tgccttcttc accaaataaa tcctgtgtat
    10381 tccagttata accggcaccg atgtctttga attgcaagcc agccgtaagc cattccaatg
    10441 gctgataaag aacaccaaaa tcgaatccga gtccggtagc gctgacgtcg agcatttgat
    10501 tatgtaaaat tttaaaatta ataccgagag agattcgggg atgaaaggcg ttagcaaagg
    10561 aaatcaagac cacgttctcg ccggtttggt aagtttcatc aacctgacca atggaattgc
    10621 gaccttcgat gtcctttact ccagcatgaa gccaggatac tcccacaccg gcatttggcg
    10681 gaagcggaac cgataatccg ataaaatgaa actggcgatc aagactcaaa aaagaatagg
    10741 aaagcgccaa gtttctttca tccatatatg ccaaaccagc tggattatag taaattccaa
    10801 aaccgttaga tggaaaggca actcccgaat tacccatcgc cgttgcgcga gctccgagac
    10861 ccattcgcag aaatgccccg gcataaccag cattgtcatt cgtttcgccc tgcaatagag
    10921 ccatcgaaaa gatgagcgat aatataagat tcagaagctt tctcataatt aattgatcac
    10981 gatcagtttt gtccagtgct ccacttcatt tccatttgat ttttggatca gtttgcagaa
    11041 ataagtaccg ttggctacaa tctcgccatt cgttgcccga ccattccaga tcaaatgatg
    11101 atctccggca tccataaccg ttttccaatc actgcggtaa acttgttcca tcgcaaaatt
    11161 atagatttcc agtcgaaccg atgcgggctg atctatatta tattgaatgc gaacatggcc
    11221 atcatcacct attacatttt gataacgcgg cgcaaacgga ttgggatagg cataaatggc
    11281 tggttgaccg gggcttttgg tcgaaacagc cgaatgataa actgtccagg tcaatccatc
    11341 gctagcggtt ttggcgacgc cgtctccggt gccgacccat aaataggttg gaccttcgcg
    11401 tcgatcaact tctgccgcgt aaaaatattg ggttaaaatt tgttcaccgg aagcaatgtc
    11461 ggcaatcggt gtataaatag cccaattttc accatcaatt gacttgaata agcctctctc
    11521 cgtacagaca tagacgattg aatcctgaaa ggcaaaatta taagggcgtt cattgactaa
    11581 ggttgtcgtc caagtcaacc caccgtcgct ggtcttgcta acagcccgat attctccgct
    11641 ttctccgact gtcaacgtgg cggcccagat ggtctctttg cctttccaaa gttggcggtc
    11701 aattgctaca acgaaattgc cggaaattgc ggaattctgg gcgttaaatt tgcgccagct
    11761 aatgcactct ggagattcaa ctatgccgag attaattcca tcagctgtcc cgatccagat
    11821 cgtatcgcca taagccagta tgctaaaacc tttatgatta tgattgccat ccggtggatc
    11881 gcgcggatta atctcgaagt ctatcagatc atcacagtcc aaattattaa ggtcatcaga
    11941 cggtaaagga attctttgcc aggtctgtcc catatcagcg ctccgccgaa taccaccagc
    12001 ccagctaacg atatagacgt aatcatcagt tatagcaata tcccaagtgg tattattgat
    12061 cggtgtcgtt accggcaaaa aggaaacact ctgattattc cattcagcac tattatcggc
    12121 tggatcgtca attggttgcg ggacgaaagt ccaatttttc cctttatcaa tagaaaatgc
    12181 cagaccgccg ccggtttgtt ggtaaccgta agattggatc agcgaatcaa aaacggccgc
    12241 cacccagatc gtatcgccct tgaccgctat tgcggaaatg ccgccgcgtg gcaaattatt
    12301 caacccggga taaaaactta cgaacgtctg gccgaaatcc gggctcatac tcaaaccgcc
    12361 gcccgtcgca aaccacatca aggaattgcc ctgactgaca atttctgtta cactattact
    12421 tgccagaccc aaaaaggtgt tgtcactatc cagggttggt ttagccagac gaaaagcctg
    12481 actaaaaccg gaggtgaaga gaaatcctat tatgaataaa atcgccggat attttttcat
    12541 tgggtaatca ccagataatc atcattgaca tcataacgcg ccggtttggt aaggtcggaa
    12601 acgcgcactt tacagtagtt ggtaacctga gtagccggta cagtccagtc ataatacttc
    12661 aaagcggccg cggttgtatc ggcgacaatc cagtctggat tggtctcatt ggcattaaaa
    12721 gtgtactcga tttttacctg actaagatag gcatactccc acataattct tagagtttgt
    12781 ccgaagcgca gggtcaaggc actggctggg gctgtaatat gaattcccgg actggcgatt
    12841 tttaatgtta tggtatcgct catcgccccg gaataatctt cagcgaagaa atagaacgta
    12901 tatagacctt ggagatcact ttcaggatta gaaggctgaa aaaccgtgaa aatgccatcc
    12961 ctggcttttt catcgccata ttctcctgca tcatttaaat aaaagtacgg atcaaaaaat
    13021 tgcccgtcgg gacgcaggat cgagtagtaa acacctacga tatcatcata gccctgttta
    13081 tcggttacac tgaaataaaa tttcaaggtg tcccattttt caaaagtcgt cggtgctgac
    13141 aacagaaaga tttccggagg actattggct tgcacgagtt cagttgcggt ggctgaatcg
    13201 ctgacattac cgtcagcatc ataagcctga acgaagaccc ggtaatatcc ttcataactt
    13261 cccaattcgg tcgaatctat taaataggaa aaaacgccgt cttgcgtaat aatatcgcgc
    13321 ggcggaccgt catctgacaa ttttccgctt ttaaaaagga aaccgttcat cgagtctatc
    13381 gtattaattc gatatagatt gaaaactacc gaatcgagat tatcccaacc ttgagcgtcc
    13441 tcgactttgg cggcaataaa caggctatga ttcaattccg aataatgtaa agtcagattt
    13501 tcgataatcg gcgtgggctc aacggtttcc acaattgact tattttcgca agtccaaagc
    13561 gttaaaatcg taataaacag ggcggcaatt attattcttt tcatttactt aatcaccaat
    13621 gccagccatt catcttcagt atcttgatca agaatttcac ggccgtaatt taagacctta
    13681 ttagtaaaaa tctcattttc ttctttaagc aggccggtaa aaatcaccag cggacgattt
    13741 tgctggcgca caagcgcctc caataatcgc aaattggatt ggcgttcgat attactgaca
    13801 atcagatcgt agttataatt tggcagggcg gttacatcgc cgatctgaat cgcgaccttt
    13861 cgagagagcc cattcagctc caaattttca ttgaaattat caatgaccag cgagtcaatg
    13921 tcccaagcgc taacctgagc cgctcccagc ttcaatgccg caattgttaa aatgccgcta
    13981 ccgcaaccgg catccaatag cacgtcgccc ggtgtcaagt atttttccag cattaatagg
    14041 gctaagtgcg ttgttggatg agttccggtg ccaaatgcca ttccgggctt tagtacaatt
    14101 tctaccgaca cgtctttaag cggtttttcc cagtaaggac gtacagcaat gcgtggactg
    14161 attgtctgcg gtttgaaata ttcctgccat ctggtgctcc aaccattgtc aacgatttca
    14221 gtgatggtga tattaagttg aggccattca gccgcaatga gctccggcaa cgacaatttc
    14281 atatttaggg gaaaatagat ttcatagtcc gtatcggttt cctgaagtcc gagcataccc
    14341 cattggatca gccgggcaat tatgagttct tcatttccgg cgtttttggg gacaagcact
    14401 ttaagccagc gttcagagtt cgcggtcact ttctttacct ttcttcagaa ccgtgaaaaa
    14461 tataatcgta aaggactgtg ccgacgattt ccaaactttc cgcactacac ttatcgggtg
    14521 tgtcgagcgt cgtgtgccag taattgataa accggttagg atattgaaaa tcaattaaat
    14581 caaccgacgg aattccggcg tgttgaatca acattaggtg gtcatcgtaa atttcctcat
    14641 caatttgatg aacgaaggca ctgaaattat gccgatcggc aatagcccag agttttttga
    14701 ttaattgggg atggctcttg accgaatttc gctcgactgg gatccgcaaa tcggcgtcgc
    14761 caatcatatc aatgataata gcttcttcgg gtttgggaat cggcaagttt ttggcaaaat
    14821 attctgagcc gaggcagtaa taatccagtg taccaggaaa tccgtaatct tcaccgtcaa
    14881 aaaacaccag ataaatagtg cggggcggtt gctgtcgaga tagaatctcc gccagatgca
    14941 tcaaaaccgc taccccactg gcgccatcat tagcgccgat aatcggagtt ttgcgtttag
    15001 ccggatcggg gtcatactcg gcgcgaggtc gggtatccca atgggcacca atcaacaagc
    15061 cattggcact tcgaatatta aaccctgcga tgatatttgt cagatttaaa acctcatttt
    15121 cctcgggaat caagtactcg aaattctgaa tgatcaaggt atcagccagc ggtctcaaaa
    15181 attctatcag cattgtgcgc tgagcggaat gcccgggcga tccgggattt cgcgggccta
    15241 aatcacactg ctgatttaaa aactgaaagg ctcttccacc atcaaatttg ccaccgccgg
    15301 atgagcatgc tgaaatcaaa attaatatta aataaaaact ttttttcact attaacctcg
    15361 aattgctata ttgacgtcga aaccgtaatt gcgggtgacg cgcttgccgg ttaacctgtt
    15421 gtaactttcg ccatactcga aaaatagacc gccagtaact ttgtcggtaa aagtataatt
    15481 gatccgtgga gtagttttcc aactattact tttggcagtg acggcaaatt tagtggcgga
    15541 gccctgtttg gcacgagttg tcgagttggt ataatcgaaa ttggctgtga aagtgatgtt
    15601 gtttttaagc tgtttatcct tcataaaggg taaaggaatg gataagccgc cttttttttg
    15661 ccagctcagg ctgatggaag cgccagcatc atctatgata gtagtaccac cggacgcatt
    15721 attgatagat tgaccgctgg tgacccgggc actggcactg atgtcattca caaattgcat
    15781 agaaagccca atcagcggct gaaagtacct tttataagac gagctcatct ctttgccatt
    15841 ttgagtaatg atagactctt tgccagaaaa gccgtggtcg agagaaagtt tcttaaagta
    15901 cttggaaaaa attttgtatt tttccagttg actccaggtc aacgaccagc tgggaaatgg
    15961 gaagccatcc tttccattga tgccggacgg aatatagtca cgggtcaacg tgcgacttac
    16021 cgtaccttga gattgggtat tcgagacatt ttgcgcaaaa cttagcggaa taactacgtt
    16081 tgagaacaaa tttataccag agcgaaccga gaaatcgaag ctgttgctgg tattttctgc
    16141 actggtggga atatattctt ccatcacaaa tggtatccca ggatcgcgcg ttagacccat
    16201 tcgatattgc caatcgggag tacccgttcg atactgattg gtaattgaat tatttagcga
    16261 aagattgaaa ttaaaaggct ggatcttttt cagatacttg gcaacgatct gggttatatt
    16321 tacttctggt ttcttgacat cgtcaactgg cttaggcatt tgttccagtg tctgtcctga
    16381 agtaggtttt aaatcttctc ctttatcaat ggtcgatcct ggttttcgcg gtcgtttttn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aataggctct ctccagttaa
    16561 aattggacgt atagttaaag gtagggttga accatttggc gattttggga ttgaaattga
    16621 ccgaataaga ctctctcata ttagtaatct gtcccggatt ggcagatttt agaatttccc
    16681 attgattctg ccataattcg atcaggtcat tttcaagatt caatgtatag ttggcgctaa
    16741 ggttatcaaa tattttataa cctaacgata aattgcggtt gagtccaaag ctattgctgg
    16801 aagttatagt tgtgccatag cgaggagtac tatatgattc acttcggccg gcatccgccg
    16861 tgactgtcaa ggcgttcggg aaataataaa agcgaatttc gctgagttta tttccgataa
    16921 gcggcaccgg agctgtccac ttgaaaatat ttatatattt atcctttgga aagggaaaat
    16981 tataggaaat gctggctttg tacgactcac tttgcgattc gcggacggtt accgagctgg
    17041 catcggtcca ggtagcgtta gcactgattt ttatctggtc tatggtatat ttagttaacc
    17101 agaagtccga agcccgcttt tcaatcgagg tgtttactcc gtaactatgc gagatgtcca
    17161 gcaggctatc gggagcattt tctttcatca gaatatcact gccatagata tatttgcgcg
    17221 ttccggtggt ttgggtatat gagccgctaa ccgggagtcg taagccccag ctggccggcg
    17281 tgaatttgtc cagagaaata cgtccggtgg ctgtcaaggc ttcggtggtt ttcaaatttt
    17341 ccgtctggag gctgggacgc tgttcaaccg tatgaaaatc cgcgtctcta tagttaaccg
    17401 aaacgtcaaa gctggctaaa tcagctacgc gcaggctggc tttggcgcgg taggctatgc
    17461 ccttgtcctt gcgaacctcc gataggcgca actcatccag ccagacctga cctttgatag
    17521 ttccggtttt aaatttattg acatatccgc tattatcagc ccggttgcgc aaaccgatat
    17581 taaggtgttt aacgtttgcc agcgagggtt tgccataaat tattatttct cgaccagtat
    17641 agtcatcgtt aaatttttct ttataatggc gaattgtaac gcccgaagtg ttagtatata
    17701 tccgatattc accatcaccg atataatcat ctgccgactc gtaatttttc agtgaggtta
    17761 gaaaatcgag atccagttcc atctcgtttt cctctgccca gccgggatag atcggttgac
    17821 gatattcata atagttcgtt gtcgtcggcg aattctcgaa tattttacca aaccggataa
    17881 agaattgaag tggggtttct tcgccttctg agaattgata accatcggct tttagttcat
    17941 gcccgtttac aaacaatttc attactttat aagtgatgta gctagtttct tcggcaagca
    18001 cttttttggc ggcgcaaaat tcgccgggga ataaaccaga accctcttca ggcaaattca
    18061 aaaccagagc ttgttcttta gtacgaatct ggttgatttc atcatattca ccctgaacac
    18121 cggctggtgg ctcataaatg tcaggattat cttcagtgtt aatgactgaa atcgaaaagg
    18181 aattttcatt gacaacgaag atggacgtat cgtctttagc aattccgagg tcttcccatt
    18241 cattacccac caattctact ttagctatga atacagaatc ttcgccggtt aagccatcaa
    18301 accagatacg acaggctcgg atgctttccc agctaatatc ttttcccgta gcgcttttgg
    18361 aaaaggcggt cagcggaatt tgatagagtt tccagccggt gggtctggaa tctatataag
    18421 tctttccggc aatatactca ttattagggt cggacagatc aaaagcggcg gtaaaataat
    18481 cattcgtcag gtctatggca taattgttgt tcagatcttc cgaattggga aaaaggccgt
    18541 tggcatcttt ttcattgttt tcagtgccgt tgatatagcg atattctttg gaaccttccg
    18601 tatatttcca gttatcggaa tgaggatcag atatatcacg tttgtattcg gttagcctgg
    18661 tatcgccata ggagagttca ccgtacttcg tggtcacaat tttttcgttt ttatcagcca
    18721 gcccgtcaat accagtgtct tcccctgggt ctagtaaacc gttgcccatt gttaatccgg
    18781 catccgggat atcttcagta tcaagttttt tattggggat ttgatcttca ctgatttgac
    18841 ctaaatcaat atgaagtgtg ccggaattgc cttttaccca aatgtcaaca tattttgtcg
    18901 tagattgatc ataataactc tggggaaaag cgtaggtaat gccgccccag gcagaatgaa
    18961 cgggagatac tccatccact accgctattg accaattcgg atcaacactc attaccataa
    19021 tttcagtaat attgttctgg gcggttgtac ttacttcttt atcaggccag atacttttgg
    19081 ttgggacgcc tccataagga ttaaaccaaa atgtgaaacc gcgttcattc tcatctttgc
    19141 caaggggaca agccgcgttt gaccaataac ggcgcataat cggcggagac gtcactcgtt
    19201 tggaactttc aaagtcatct ataaaaccga cgccatggcg atctccagtt tcttcgttat
    19261 tgagagtatt tgggttaggc aagacctgtg caacctcgcc ctcgatatca aaggatgaag
    19321 ccgcattggt gctgactaat ggcaaccagt taaccgctcg cgttagaaaa tccagattac
    19381 gattatagcg tccattgata tcccatacaa aattgtgcat cggttcgtaa ccgacttcga
    19441 ctttttcatc actcactgat tgcccgaaat aaagagcagt ggcaccgatg tagctgtttt
    19501 caccaaaatc gtattgtgct ctggcaccaa ggatcgtttt tttgtccaat tggaaaaact
    19561 gattcttttc atattttact tctaatttcg catttgggtc ggttgcatct gatgttagaa
    19621 gagttaacgt tcctgaaaaa taatctataa tgtaatcttt cccccgctcc agagtactgt
    19681 ttccaagggt tactgtttca ctattttcga taaccatagc cccaagattg agaatcgaac
    19741 tgcgattttt atagcttacc ataatcttga atttagtttc gcttaaaatg tcattatcgg
    19801 ttcggttgct atcgtacatt gccgaacagt tatatatttt gctaatatta ggattacgtt
    19861 tgccggcgtt atctgtcgag tcgtactgaa atgggcgtaa atagggaaac cataattcac
    19921 cggaatacaa gtttaggatc gctccattat tcatatcaat aacaccatcg ggagtgggat
    19981 taccgttatt gtcaacccga tctagtccga acatctccag gaatttctta ccggtcacta
    20041 aatcgtaatt ttgctcaccc tcacccaaag tataaacgat atccagttca aatccgtcac
    20101 tatttatgcc ggttgtaccc aaataataga tatttttgaa ttccagattc cagcagggat
    20161 gagtgggaag catttcctga ggtttaatca atttcaaaat tatatgagcg gtagaagtat
    20221 cagagcgatt ataatctcca tattcttcgc cggtgacagt tacgttatta tcgtcagtca
    20281 agcggtaggc aactgccagt atttcaccat cctgtacttg agttgtcatg cgaatatatc
    20341 ccaaagtcac atcgacataa taatccttat tcaattccag taatttaaaa atgcggtttt
    20401 cgctataatc tttataaaga gtagtatcgc ggggatcaac ataggcggta ccataggcac
    20461 aaccggcagt ttcgatcgtc tgagatttat agacctgcaa atccgttact ttccctcttg
    20521 gatcagcgtg aaataattga tcgaactgat aacccctata catattatcg cgaaagagag
    20581 tgtcgaggaa aaaataaaga tttcggcgat attcgtattc tttgatgcta atgttcgagg
    20641 tctgcgcccc gccatctata ctcagttttt cttttttgcc tttctcgacg ctggcgattg
    20701 cggtaacatc aacgccgcca aatttcatca aagctttaag gccaaacaag ccattgtttt
    20761 tgcccgaaaa agttacgaat tgagtaccgg gcaaggacag agcgacatta ccggcttcca
    20821 ctttttgaac aatttcatcc tctttgccgg tataaataat cttcatagca ttttcaaaat
    20881 caaagtcgcg ctcactatcc tggtcaacca atatgctaat tcggtcgcca atcttaccct
    20941 caatgttcaa ttgctgtttc tgatcaacga taaaactcgt cgaagttcct tccgcataac
    21001 tactgctcac ctgtgaacgt ttctgggttt gcagacgtcc gttaatatta atattaccgg
    21061 aaacgcgcaa agccacgtgc tgaccggcaa tatcggcacc gattagctcc agcttatcac
    21121 tggcggcttt agtgtcagcc ggtttggcgg taacaacttc gactaattta ttatgccaat
    21181 tcaggcgctg tctgagatca aaatagctag caactggaat tccaatggga gtaaggactg
    21241 gagcaccgtt aagatcattg taaaaaataa agtgctggcc ggatttatcg agggtattag
    21301 tcaccttcat cccgggaaat tggactttga ggatattttg gttcaatgtc gcataatcgg
    21361 gtgaaatcaa cgacaaaccg cgtttgcttt cgatattagc tggcgtatag cgattagaca
    21421 gccagtcaat ttttaaagga aatacgtccg gctctctgtc cgcggccaaa ctttggacaa
    21481 agaaccccat taaaataaat atgggtaaag tatatctttt tatcagattg atattaattt
    21541 actaaccttt taatatggtg gttatggtgg ttaaaccgac gcaaagctta ttgcgcgcag
    21601 ggtagcaatt ctaaaaaaag tacctcaaat attgtcggtt acaaatttat caacggattt
    21661 taatttattg gctaatcgag tgaacgcctg accgcgatga ctaagggagt tcttttcgct
    21721 taagtccatt tccgcaaagg ttcgaccgag ttgtggtaga taaaataccg gatcgtaacc
    21781 gaacccgcca tttccgcgcg gttgacgtaa tatgatcccg tcaattttcc cttcttccac
    21841 taaagtcaaa tccggactgt agtaaaccgc tacacagcgg aaatgcgctt gtcgctcgga
    21901 gtcaggtata tcccgcataa cttccagcaa tttggctaca ttttgagcat acgtagcatt
    21961 ctctcccgca aaacgcgccg aaaaaacccc aggctgtccg tgcaacgcat ccacttccaa
    22021 gccggtgtca tcggcaatcg tcggtaatcc ggtaaattca gcgatcatac gtgctttctt
    22081 aaatgcattc tcgaaaagcg tgttcccgtc ttcaacaact ttgggacagt cgggatattc
    22141 atccagagtg tgccatatca gctccggtag agaaaaaatt cgcttgattt cgcgcacttt
    22201 atcccgatta tgagtagcaa tcaaaatgtc catttacgtt ctctaagcat ttttaattaa
    22261 gcggaaaatg ttatgcataa attaagttaa tgtcaagctt tgacaaacaa aaatctaaat
    22321 aataaattca ctttcaagat aataaacttg ttttccaatt gaaaaattac tcagatgctt
    22381 gtttcggagt agaaaacttc taaattgaac gaaattttat cattaaagca tataatttta
    22441 caattactaa attaacaaat agttatagcg gctctagtta tttcactatt ggattcttta
    22501 gtcctgtaac tcctgccaac gaaaacaact ctcgagatga tcgttgacca tccccgccgc
    22561 ttgcataaat gcataacaaa ttttcggacc aacgaaccga aagccacgcc gaatcagatc
    22621 agcgctcata gcttcggctt ctaaactttt gtccggtagt tccgataatt tttgccagtg
    22681 gtttacaatc gttttcccag cggtaaactg ccagatataa cgatcgaatg acccgaactc
    22741 cagttgaact tctaaaaagc gtgcggcatt atttattgtc gcctggattt tagcccgatt
    22801 acgaataatt cccgcatttg ccagaagcga attgatttcg atttcaccaa aattagcaac
    22861 ctgatttacc tcgaagcctg ccagagctcg gcggaaattt tcgcgcttgt gcagaatcgt
    22921 acgccagctt agcccagcct gaaaactatc caatactatg aattcaaata atttttggtc
    22981 attatgaagc ggtacccccc attcggtatc gtgataatga atcatcagct catcattagc
    23041 tggccagggg caacgtataa tcttagtcaa attgaaatct ccatcttact ctactgttaa
    23101 attcaaattt ataaaattat ttaggtattt agacaaaatt cacaatttta tggaaatcac
    23161 caaaaaatag atattcaaaa aaaacaggac attaccctcg ccttttagag ataaacaaga
    23221 ttagcaaaag cttgttttcg aactcttgtt taccgctgaa taagtccagt aataagttta
    23281 gaataacgag caacttttat cattcccaaa aaattaggtg caatcctgtt aatgttggga
    23341 atgctatgat tttgcttttg tatcttttgc tttctatcgc gcggcttttt acattacttc
    23401 acaattggag aaagataaat ggatatagta gcattggcta gaaaaaaggg attattgttt
    23461 gatggagctt tcggctccat gctgattcag catggtctac ctacgaacgc gaacgccgag
    23521 gtctggtgcc tggagcatcc ggaagtggta accaaaattc acgccgaata cgtcgcggct
    23581 ggggcagacg ttctcacaac caatactttt ggggcatcat cgttaaaatt acaaaaggca
    23641 ggtgtcgaga aacgcgtcgc cgagattaac catcgcatgg tagaaatggc gcgcgccgcc
    23701 gcaaagccga atactttaat cgccggtaac attgggccaa ccggtgaaat gttagcccct
    23761 tttggaaaaa tcagtattga agaagccacc attaattatg ccgagcaggc tggttttctc
    23821 gataaagccg gggttgattt attcataatt gaaacaatgt ttgacctgaa tgaagctctg
    23881 gcggcaatta aaggcattct gcaagtgtct gccaaaccga tttttgcaac tatgactttt
    23941 agcgaaagcc cgaccggttt cgtaactatt atgggcaatc cggtggaaga aagtatgcgc
    24001 cagctagtcg aagccggcgc tacggcagtc ggtgccaatt gctcgatcgg cagtgaaaaa
    24061 atggtcagct tagcccaaat aatccgcgcg gctgtatcgg ttccagtggt tattcagccg
    24121 aatgccggta ttccaattct gcgaaatggc atattacact atccggaaga tgttgctttt
    24181 tatacccgca atattatgac tatcaaacaa gccggtgtcg aaatcgtcgg cggttgttgc
    24241 ggtacaactc ccgaatatat tcgcgctatc cgccaggcgc tcacttaaaa tttcagtcga
    24301 aaattttcac aataaattct cttagttccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24421 nnnnnnnnnt aaattttacc caaactctcg aatgtcaata ctgccgcgag actcgcgaat
    24481 tgctggaaga aatcacggca ctttctgata aattgagtct ggaagtttac aatttccaga
    24541 ttgaccgttc tgtggcggaa cgatatggcg tggacaagat tccggctacc attttgtttg
    24601 ctgaaactgc tccccattct cggattaaat tttatggaat tccagccgga tacgagtttg
    24661 ccgccctact ggaagatatt gttgatattt ctaataataa aaccgaccta tctccggaaa
    24721 tcgtaacaga cctaaaaaaa gttacctggc cagtgcatat tcaggtcttt agcaccccga
    24781 cttgcccata ttgtccggcg gcggtgcgca ctgctcatcg gttcgcgctt ttgaatgaaa
    24841 acattcgggc tgatatgatc gaagccatcg aatttcccca tcttgcccaa aagtataacg
    24901 ttcgcggtgt accgcgctcc gttataaatg aaaatataat cattgacggt gctgtctcag
    24961 aaaaacaatt tctggaaaaa attttatcca gccagccgac aacttgatta tcgagattaa
    25021 aataccaatg gaattaacga tgttattcat taaatttacc atcgttggaa aacaatctac
    25081 cggaagaaga attagttgct cgcgccaaaa agggcgacca agcggctctg tctcagttgg
    25141 ttctcaatta cagcagtcgc atttatcatc tcgggttgcg tttaactggt agtcatcagg
    25201 atgctgagga cattttacaa gatacgtttc taattatggt gaaaaagatc aaccaatttc
    25261 agggcaaatc atctttctat acttggctat atcgcatcgc gatgaatgtt ggcttgcgaa
    25321 agttaaagtc caaaccacgc aaatatgaac atatttcgct tgacgatccc gattttgaac
    25381 gagtaagttc cgaagccgcc agcgaatggc ctgatttcga ttatgatatc gtcaatgatc
    25441 ctcactttcg ccaaaggttg gatcgtttga ttgaacaatt acccgaaaag tatcgcacag
    25501 ttttcattct gcgtgattta catggattat caaccgaaga caccagccgt attttgcaga
    25561 tcacaccttc caacaccaaa attcggctga tgcgggctcg taatttcctg aaagaaaatt
    25621 tggaaaaaat gctgaaaacc gaaatgcagt tatgaagcta gacgaagaac ttctcaagga
    25681 aatctgcgag tatatgggta gtgatctaaa ctccgctccc tgtaaaatta tccgtgatca
    25741 tatggaagcc tgcccgaatt gtgaagttta cattgacaaa attaaaagaa cgattgaaat
    25801 ttaccgcctt gccgaccaat gtgaggaaat cccggaaaaa gtcactcgta atcttctagc
    25861 ttgcttaaaa cttgacgatc caagcaagac ctgtaaaaaa taatttcacc ctttcttcag
    25921 aatagattac aatagccaaa atcaaaattt ttggcgtgct ttgctttttg accacagaaa
    25981 cacccgggca aaaaagggct aaggctgaga a
//
LOCUS       DAOMJU010000023        25694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1765_length_25694_cov_24.990912, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000023 DAOMJU010000000
VERSION     DAOMJU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 25694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25694
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1765_length_25694_cov_24.990912"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..2959
                     /locus_tag="PLO58_03635"
     CDS             8..2959
                     /locus_tag="PLO58_03635"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HQO73915.1"
                     /translation="MSKLGGEKYSVQQPIIDYVREQSAEYVAQNGARVFLKLGWQYVS
                     PDEALRLRGGETGMVFKEVFSDQLQKLNPDFKDSSMAEEIVKKIESVPSNIEGNFIAW
                     EYLKGLKTVFVPGEKRERNIKLLDTENLDNNVFHVTDEFKFTNGIKTIRLDIVFLING
                     IPVLFIETKSSDKIEGMSEALEQVKRYHRECPELLAVLQVYALTHIVQYYYSGTWNTS
                     ERFLFNWKEESGGDFEHLVKTFFDRERVVKLLVDFMMFTRQDDALLKVILRPHQMRAV
                     DKIVERAKDPEKHRGLVWHTQGSGKTYTMIIAAQKIIANPLFENPTVIMLVDRNELES
                     QLFGNIAALGIGNVEVTESKEHLRNLLSSDRRGLIVTMIHKFEGIPANINTRKNIFVL
                     VDEAHRTTGGKLGNYLMGALPNAAYIGFTGTPIDKTQYGNGTFVTFGKDDPPQGYLDK
                     YSIAESIEDGTTVPLYYTLAPNELRVEKEALEKEFLNLAEAEGISDIEALNKVLEKAV
                     TLKNMLKNKERVEKVARYICDHYKNYIEPMGYKAFVVAVDREACALYKEELDKYLPPE
                     YSEVVYSSAFNDSPELAKYQLSEIEEKRIRKAFRKSNELPKILIVTEKLLTGFDAPIL
                     YCMYLDKPMRDHVLLQAISRVNRPYEDDEGRKKSSGFVLDFVGIFDNLEKALAFDSKD
                     IEGIVRDIQVLKNRFENEMKLGREKYLTLIKGKTQDKAVEAALEHFRDEEERQEFYKY
                     FKKLSDIYEIISPDEFLRSYLDDYETLLRMYKIVRENYERGTLIDKMFTRKTALLVQK
                     HTTSSDIRPTLEVCEINETTLRRIEESKASETEKVFNLIKSIERTVEEQSGNSPYLFS
                     IAEKAELLVKMYKMRQKNTQETLEELKRIIKEINDARKEQTEKNMSAENFTIYWMLKS
                     EGFKDAEAMANEMRNLFEKYPHWQKSEKYEREIRTGIYQTITRSGVKDVKEIPEIVKK
                     IMKTLKANREE"
     gene            2956..3231
                     /locus_tag="PLO58_03640"
     CDS             2956..3231
                     /locus_tag="PLO58_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQO73916.1"
                     /translation="MMTPEKFKENVLTLANDVGVKPREIHIQPMKRKWASCSSKGRLT
                     FNKKLLKEPEEVRLKVILHELLHLKYPNHGKMFKLLMDTYLQKSLLK"
     gene            3303..3995
                     /locus_tag="PLO58_03645"
     CDS             3303..3995
                     /locus_tag="PLO58_03645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HQO73917.1"
                     /translation="MNPTIQEMFTTVPPTYRILNQILTLGQDNVWRKRAARLATSAGG
                     RRWLDACGGTGEMAIELSRLANYGTSIFVADFSLPMMGKAREKSEAKHIIFTLANMKC
                     LPFQSDTFDVITISFATRNLSISQDNLLQCLGEFQRVLKPGGLFVNLETSQPRWTPIR
                     RLFHLYVKATVKPIGRLISGSENAYSYLSHSIRHFYSPEELAEIIRQAGLREIGFKRM
                     FLGAAAIHKAIK"
     gene            4258..5445
                     /gene="kbl"
                     /locus_tag="PLO58_03650"
     CDS             4258..5445
                     /gene="kbl"
                     /locus_tag="PLO58_03650"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HQO73918.1"
                     /translation="MYGKLQQDLQSQLESITKQGLYKSERVIASPQAPHLRLTDGREL
                     LNFCANNYLGLSNHPVIIQSAQLALERWGFGLSSVRFICGTQEIHKELERRLSDFLGT
                     EDTILFSSCFDANGGVFEPLFDEQCAIISDELNHASIIDGIRLSKAQRFRYSHMNIDE
                     LQKCLENSAQAKYRIIVSDGVFSMDGHIAPLPQICELAEKYDALVMIDDSHATGYLGQ
                     TGRGTAEYYGLLGKIDIITTTFGKALGGASGGCISGRREIVEFMRQRSRPYLFSNSVA
                     PAVIGATLKALEIVQENPDLPRRTIENAHHFRQKIEAAGFDIIPGNTAIIPIMIYDEP
                     LAIKIADLLLEEGIYVIGFTYPVVPRGTARIRVQVSAAHTPEQIDRAVAAFTKVRKSL
                     NKG"
     gene            5547..5966
                     /locus_tag="PLO58_03655"
     CDS             5547..5966
                     /locus_tag="PLO58_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQO73919.1"
                     /translation="MALSKGSVNKVILVGRLGRDPEVRYTPNGDAVAEVSLATTESVK
                     NRDGKREDRTEWHNLVIWRNQAEFAKEWLKKGQLVYVEGRLQTRQWEDKEGQKRSKTD
                     IQVDQITMLGGSGRGREKEAAEPETPPEPSPDDEIPF"
     gene            complement(6042..7808)
                     /locus_tag="PLO58_03660"
     CDS             complement(6042..7808)
                     /locus_tag="PLO58_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HQO73920.1"
                     /translation="MLFLASIEQSFTTNLKLLIYLQKPNFIFQYFSDNFQSMEMIEVN
                     LGETRTITVQPGQKIIEIVKQALPHNRYKPYIVIRNNTFTSFNEIVNEPTKISLLRHF
                     HESSRRAYESAAILILNYVVEDLYPDQRLIVMHSMCDGVFCRFVNEDFCTPQVIQEIS
                     AAFIKVVEADYAIEPLLISCNDARRYFKEIGQEDTEELLKYSEANFVTLYQINGRRFW
                     LLSPPAPCTGLIKVFEIRPYQKGFVLRCPTEGDDRRLRPFTDQARLYEIFQETEEWGR
                     ILEISTVADLNRTIVENQISDLIKIDEALHEKKIASIADQISNKGERRFVFVAGPSSS
                     GKTTFAKRLAIQLRVLGFKPLLLSLDNYFRDRAELAKLQGKRINYEVLDAIDLELLND
                     HLQRLLRGETITPPVYNFITGTKTPATKPMKIDSQTILIFEGIHGINPGLTPEVNERY
                     KFKIYVSALTHLNFDHMNRVRTHDTRLLRRIVRDYKYRGYSAAETIEMWKKVVEGENK
                     YIFTFQGNADIMFNSSLVYEHGVLKTLAVTMLKNVPESHHSYPEAQRLLDMLSYFLPV
                     EDTEIPPTSIMREFIGKSSFTY"
     gene            7821..9200
                     /locus_tag="PLO58_03665"
     CDS             7821..9200
                     /locus_tag="PLO58_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO73921.1"
                     /translation="MNTSETENLLAMPTFRHRRLQNWIIIGLLYSFFYMSRYNFSAIA
                     PTLQAFFGWTKSNLGVFETLMPLVYGLAVVINGPLADKIGGRKAFLIGALGVVIMNGL
                     FGFFHILVQTPAVWADQEIVSEAVLKFGFSKSALLWTLAIIWAINGYFQSFGALSIVK
                     INAQWFHIRERGTFSAFFGVLIRFGLILAFSGTPLIVKFLPWYWAFWIPALVVALLFV
                     LTIFFVRDTPAEAGFPELDTGDGDVNLEEKVKFKEVLTKVFGSKVMWTIAIGSMMIGF
                     VRRGVVDAWWPIYFKEMQGVTGVDFAFQLTAWGIAILGIIGGFAFGISSDRVFGGRRA
                     PVIVIGFCGMIVILTLFFLADVWQWGPIAAAILLACLSFFVNGSHGMIAGAATMDFGG
                     RKAAATAVGLIDGMQYIAGAFVGALVGWVTTNWGWQAWKIWPIPFAILGALVMSSIWH
                     TTPTGKSTH"
     gene            9219..9941
                     /locus_tag="PLO58_03670"
     CDS             9219..9941
                     /locus_tag="PLO58_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73922.1"
                     /translation="MDNIFDIIILNGRPASGKSEVIDYLKKTPVEIRRRRFHIGELDE
                     IDDFPMLWTWFEEDAILEKILNKPRLHSDKDGYFFWEYLWHLLIERISMEYSKRLRDK
                     PNFHNNYTALIEFSRGSEHGGYRAAYPHLSDEILKRAAIFYIDVSFEESLRKNRQRFN
                     PAKPDSILEHGLPDAKLTRLYKDCDWEEFRGNDPEFIKIKGLKVPYVVLHNEPDITTA
                     RDENLGRVLEELLNKLWILRQK"
     gene            10036..10875
                     /locus_tag="PLO58_03675"
     CDS             10036..10875
                     /locus_tag="PLO58_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQO73923.1"
                     /translation="MLKPLAIESWPDMFFYRPLAFGLVKTIIPLPITPNMISLSAIII
                     GLSGGYCLAIGTPRICQIAGLLFMANVILDCADGMLARYKHNGTPLGFIVDGLVDYCN
                     GAAIFIGMTVGFSRTDSQFGFSLWVLIPLSLFSWIAHSMAVDHARREFQRNALEQCPS
                     LAGGRQNFQNYREQLSRSKGHWSDKLLASLYLFYCNVQKPNAKFSRTYPADRYYRYNN
                     CVMRGWLLIDPSTHMTIAILSALLYNPLIFFWYTIVIANIIMLMMYLIQYFVNQKVGV
                     LPV"
     gene            10875..11861
                     /locus_tag="PLO58_03680"
     CDS             10875..11861
                     /locus_tag="PLO58_03680"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQO73924.1"
                     /translation="MKALVTGATGFIGQHLIRRLLAENCEVRALCRSTSIKPDEFAQI
                     VEWQIGDITAPESLASAVSDCDLVFHLAGAIKAQDDATFTRINVNGTVNLLKALKQYG
                     KKEVRFIFVSSLSASGPSEPWSPKNESEPCYPVSAYGQSKLEAEIEVLKQKNRIWCTI
                     IRPAVVYGPGDKESLAFFKIAKSRFNPHLGFEKRYVGMIYVGDLVDLLWKAALSNRLS
                     GEIYFACDEQSDGYEWNQIIATAGKVLGYHTLPILVPQFILPMIVVPTQIIAKMTGKI
                     GSLNRDKYREMNQSHWTCTSAKAQKLLDFKPQVDLSEGFKRTAQWYREQNWI"
     gene            11852..12781
                     /locus_tag="PLO58_03685"
     CDS             11852..12781
                     /locus_tag="PLO58_03685"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQO73925.1"
                     /translation="MDLTPESPTPLINNYKKGVFPADCLVFGFTVLMLLIGITNYSRL
                     GKPYTWISTDCGIIIVLLMLIFKETHEERRFFYWLHFFWPIISLAFLYTQCTSWDNLI
                     FPITFDPVLIKWDLRLFGSALNKVMASSVDSLWLDEIMHFFYFSYYLVIFLTVVWMLG
                     RRQPQAYELIFSLTLMMYFHFIFFMIFPSDGPLADRNDLFNQGVVFIPLMDFIYGVSE
                     QNGGGAFPSTHVSAAVVVAIYTFKNSPKLRIPIGGLCLGIIISTVYCSYHYAIDAAAG
                     IITGTLFYFLGKYIYNHRFQPVNLSLFMPQKSI"
     gene            12806..14863
                     /locus_tag="PLO58_03690"
     CDS             12806..14863
                     /locus_tag="PLO58_03690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQO73926.1"
                     /translation="MKSKAVGLLWLSLIIMVEIGFTAKRSMQIDDLLNCHRLAEPQYS
                     PDGQWIAFTVQTIDIEANKGQKTLWRIRPDGQNLQQIPTHSGNSWSPLWSPDSKSLAF
                     LSDSGGQVQVWKLALDNNHVQQITDFYGGVESFCWSHNGQKIAFSARVYPDLTDFDEM
                     KKRDATRQASLIKARVYEELMFRHWDEWWDYKRSHIFVLDLNNGSLTDVTPGDFDAPP
                     IALGEGYCFSPDDQTIVFNSNHDPVIAISTNNDIWSASIANPREPKLITTAQEYRDFR
                     GNDYAPRFSPDGKYLSFLSMARAGFEADKSDLFLYEMTTGKFINLTEKNDIRIQAYEW
                     LPDSRGLLLLIEERGRLTLKTLNIKSGQIKTLVKEGFNSAVTLAPDGRSCAFLQSRTT
                     SPDELFVYSFADNKIRQITDFNSPLRSQIEMNDVEDFEFKSSDGAMVHGFLITPPFFD
                     AREKYPAVFLVHGGPQSAWEDNWHYRWNPQLWSAQGYVIIMINPRGSIGYGQKFTDAI
                     SLDWGGQVFDDLVVGQKYVIDHFKFIDADKLAAAGASYGGYMMNWMEGHMEAFSYPFK
                     TLVNHDGSFNLYAMYLTTEELWFPEWEYGGVFWENRDQYEKFSCDNYIINFKTPMLII
                     HGEKDYRLDPSQGIMAFTALRRLNIPAKLVLFPDEGHFVLQPQNSRFWHQTIFSWLEK
                     YLR"
     gene            15008..15715
                     /locus_tag="PLO58_03695"
     CDS             15008..15715
                     /locus_tag="PLO58_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQO73927.1"
                     /translation="MNTIFSSTYDIGIMGWVIILILIGLSIIAWGIIIEKMILFRRTE
                     KQNKKFLAIYQSYSDWSAVARETQHLKSGLLLNLFRKIYAEARKDAELNPGNVVTENI
                     DTDASRISSLELYKALVDSAISTELIPYEKRLIFLSSTVSASPFLGLFGTVWGVMQAF
                     LSIGVKGSAAITAIGPGIAEALITTVVGLAVAIPVLFAYNLIVDKIRKYENQLQILAN
                     DMIQKIIRENFYGGSQI"
     gene            15696..16100
                     /locus_tag="PLO58_03700"
     CDS             15696..16100
                     /locus_tag="PLO58_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQO73928.1"
                     /translation="MVDRRYKPLTEINITNLVDVTLTVLIIFMITTPLMYSGIEVDLP
                     ATRREITTVQEGILVNYSLDGQIFIDGQPVIENEFEKTLVKRWVQVGKKPVLLSADRK
                     IEYGKIIKLIDRIKACGVTELGLIVETEQDKK"
     gene            16097..16777
                     /locus_tag="PLO58_03705"
     CDS             16097..16777
                     /locus_tag="PLO58_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQO73929.1"
                     /translation="MNYTFAISLILHLTFFAVVSVLAGRHQPRVMAIPDQNIRWVNIA
                     PPVVQTAEAPIQVKPATSVPVPKPKVEKAATVLAEKPQKPQETKPLPEETIASSTTSP
                     ELPVEPEATSQPASGGIPGATGLRVDDPDFNFIYYLNIIRNRIQEHWRPPYSSADSPY
                     SQQVMIAFKITRSGKISDVRIEQSSGNFLFDQAALRALYETATLPPLPSEYGGKELNV
                     HIEFETLK"
     gene            16854..18047
                     /locus_tag="PLO58_03710"
     CDS             16854..18047
                     /locus_tag="PLO58_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQO73930.1"
                     /translation="MFLKIQSDETRKINVAIAEFGTNQNPELAINLRQIIIDDLNYSG
                     FLNVVDPLISPAGDLIFDNSEVIITGDVTHKSSYFNFVFGLTESVTGRKLMTKSLRSY
                     PFALRVLAHTVSDEIVYQLCGEYGNACTKIAYVTEQKGGKELALMDWDGQRATLLTRN
                     RSINLLPCWSNDGESLIFTTYAFGGGPILARYIFKDQQIERFIARAGTFTSPEYSPNG
                     KKVAFVLTHEGNADIYTADRNGQNLQRLTSNPAIDTAPSWSPNSREIVFTSDRSGSPQ
                     IYIMDAEGGNVRRLTYENNYNSSPKWSPKGDQIVFVSNQSRGFQIYLIDVTGENLICL
                     TDNTGSYENPTWSPNGSHLVCASNRSGRWEIYSMLRDGSLLRQLTFTGGNTGPAWSPR
                     LKPFN"
     gene            18108..18722
                     /gene="pal"
                     /locus_tag="PLO58_03715"
     CDS             18108..18722
                     /gene="pal"
                     /locus_tag="PLO58_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HQO73931.1"
                     /translation="MKKLLVFLIPLLCIVLMFNTCSKKTAGVQEDKGETPAKVTPAPE
                     QPKPVQKPDTLAQAPAPVVTPPPAEPKPVIADTQKVAAIAEPSLKDELAQCNTVYFDY
                     DRYDLRPDAIATLEKAAKVLKQYPNAIITIEGHCDERGTVEYNLALGERRAHAVKTYL
                     INYGIKPDNLITISYGKERPIDPGHNEEAWAKNRRAVLVTSDSK"
     gene            18754..19584
                     /gene="ybgF"
                     /locus_tag="PLO58_03720"
     CDS             18754..19584
                     /gene="ybgF"
                     /locus_tag="PLO58_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HQO73932.1"
                     /translation="MNFKPIRPIIYGLFVISLTACATQKDLLKIQEDLYFLRNQIQSV
                     KQTTEENKQLLSQVPTIAVQCNQILIELDSLEKSLAELQDANDRTRADINTRLSSLKQ
                     DAAIISSKLDDASYRSSQLIGKVESLTGKMTNISEKIESRAATPNSAEPAPTEIFNNA
                     YRDMTKGNIELAIQGFQTFLKLYPEKELADYCQYYLAEIAYQQEDYATAATEYKLLIN
                     NYPRSQKITNAHYKLGLCYLKLNNLEQARTQFETVIREYPNTEEALLARNQLQKLEQQ
                     "
     gene            complement(19606..20625)
                     /locus_tag="PLO58_03725"
     CDS             complement(19606..20625)
                     /locus_tag="PLO58_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQO73933.1"
                     /translation="MSFEKLIKPGTEFLGVKYPIICGAMTWVSDYNLVKTVSETGAFG
                     VLAGGNLPPDLFEQEVDRCIAGVKKPFAVNLITIGPNFRIHYEILQSKNVPFVVFAGG
                     IPRRQDIEGMKTRGKRTLSFAPEESIARQQIEYGIDALILEGSEAGGHIGYVSLIVLL
                     QQVLFKVKGLPIFVGGGIADGRMIAHLLLMGAAGVQMGTRFVLTEECRVHPNFKAALL
                     KAKARQAIATPQYDSKLPVVAVRAIKNKGTDEFGKLQMRLLQEIAAGKITREKAQYEV
                     ENFWVGALRRAVVEGDVDYGSLMAGQSVGLINEIVPMQKMINDLIKEARAEIKVIRNR
                     LGQCG"
     gene            complement(20731..21636)
                     /locus_tag="PLO58_03730"
     CDS             complement(20731..21636)
                     /locus_tag="PLO58_03730"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HQO73934.1"
                     /translation="MLKTYSEIFEKVKKNARRPTIAVALAEDKSVMEAIKVLSANDIA
                     RVILTGNPKKIKSYAEQVGYPIKEDEIISAFSEEEGATRAVELVHLGKAEILMKGHIT
                     TPALMKAVLDKEKGLRSGDLLSHITVVAVSNYHKLLMVTDGGVNIAPDLEKKKAIINN
                     AIIFAKKLEITRPKIAVLAPIEKVNPKIPETVDAMTLQEMAERGEFGSAIVEGPVAAD
                     VALSARAAERKGLHSRIAGDTDIILVPGITCGNAIIKFLMLLAKAEVGGVVVGGRVPI
                     VLLSRSDEPAEKLNSTLLALLMSAL"
     gene            complement(21640..22725)
                     /gene="buk"
                     /locus_tag="PLO58_03735"
     CDS             complement(21640..22725)
                     /gene="buk"
                     /locus_tag="PLO58_03735"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HQO73935.1"
                     /translation="MIETVLVVNPGSTSTKIALFDRSRRLQFENISHVGTPISRIHSI
                     AEQLPLRIEIVTNTIKPWLFDYCLKAVVGRGGLLKPLRAGVYKITPGMITDAISCKYG
                     EHASNLGAPIAEVIANQHHISSFVVDPVTVDEMIPEARISGIPEIERVSRQHTLNIRA
                     CVRKASAQLGRLPSELNFVVAHLGGGVSIAAVRGGRLIDCNNALLGMGPFTPERAGAL
                     PLEGILNLAYSGKYTKEEMKIKMTKKSGLVAYLGTNDAREVWQRAETGDQRAALIFRA
                     MIYQVCKEIGAMATVLKGNVDAIIVTGGQARGAETVEMIRAGVGFIAPLIVFPGENEM
                     EALAEGGWRALDGNEEILIYEEINVKE"
     gene            22967..23749
                     /locus_tag="PLO58_03740"
     CDS             22967..23749
                     /locus_tag="PLO58_03740"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQO73936.1"
                     /translation="MNQNVYFIADIHLHLNDSQEEKVKRERLFAFLAEVRRHQGSLYI
                     LGDMFDFWFEYRYVIPGVFFPVLCELRETVLAGCEVHFLGGNHDYWAGQFFTDVLGIT
                     VHYKPIDTIIAGKSFHLTHADGILHNDRGYRLMRFFFRNKTIIRLFRYLHPDCAIRLA
                     THLSGRSRRLTLRSPAQEESDRQEIIAYGKKKLEAGAEYVITGHLHLPTEYHTERGVV
                     VNLGDWMKYYSFAFFDGKSLRLCYWPLSTSDSTGKKSEFLLD"
     gene            23803..24897
                     /locus_tag="PLO58_03745"
     CDS             23803..24897
                     /locus_tag="PLO58_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73937.1"
                     /translation="MKAKNIIIIMLVLGGLMSCAPETATIDPRQVEKLRVDYNRGKIQ
                     KLDDLIAIYQDQAQPIETRIAAVNALAETQHPDAIKAMHDFMDQSVGLNYALLNATAT
                     ALTKHATPENVAAMVDGIVSAQKKYINFRTGVMEKMEDLNVALQVEALLKLYQSEKEN
                     YIFMQESLTKLLGSIPDDRVIPILINIAKDESVNISTRSLAMEILGKKRHPLITETFI
                     EMLGDPEQQLALREFAIKSIEDVKESRVILALLETLNQSREEHLVLLDALTRAMGDYS
                     DPAVIPALVEIAKNTDYPVATRRNALSALVKFQDIEAFKQLLPIMESPEGYVLYDEMA
                     QIATAIGQPEVLNQLRLAALKAQLKAREAQ"
     gene            24894..25607
                     /locus_tag="PLO58_03750"
     CDS             24894..25607
                     /locus_tag="PLO58_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73938.1"
                     /translation="MKKQILYLIPMICAVFAVSSFAQSTSPKSATTDKFFEVDQSIEE
                     LRQEIAEIRRQIRDIELRTSTPEIRKEINRLIPVPDITHEIILHNGTVVQGKIIHETI
                     DKIVIQTNIGQLTISKNDIHVFREAEKPHAKCVLVGPITPKIFEDKTTYTGKIKNEGA
                     RRADFPQIKIQLFDEATNLIAADSIIIAGNYHMFLSGVQTDATIEPGQTFDFEITISY
                     PANTKVSYFIPKITWQEFD"
BASE COUNT         7169 a         5842 c         5626 g         7057 t
ORIGIN      
        1 taacatcatg agcaaattgg gaggtgaaaa atattccgtt caacagccca tcatagatta
       61 tgtgcgggaa caatcggcag aatatgttgc gcaaaatggc gcaagagtgt ttctaaagct
      121 cggctggcag tacgtttctc ccgatgaagc acttcgcctg cgaggcggag aaaccggaat
      181 ggtgttcaag gaagtcttca gcgatcaact tcaaaaactc aatccagatt ttaaggattc
      241 ctcgatggct gaagaaattg tcaaaaaaat cgaatccgta ccatcgaaca tcgaagggaa
      301 ttttatcgca tgggaatatc tcaagggttt gaaaaccgtg tttgtccctg gcgaaaaacg
      361 ggaacgcaac atcaaattac tcgataccga aaatctggac aacaatgttt ttcacgttac
      421 cgatgagttt aagtttacga acggaatcaa aaccatcagg ctggacattg tctttctcat
      481 caatggtatt cctgttttgt tcatcgaaac aaaatcatcc gataaaatag aaggaatgag
      541 cgaggcgctg gagcaggtca agcggtatca cagagaatgt cccgagcttc ttgcggttct
      601 gcaagtctat gcattgactc acattgtaca atattactac agcggcacgt ggaatacatc
      661 cgaacgtttt ctctttaact ggaaggaaga gtccggcggc gacttcgaac atcttgttaa
      721 aacttttttc gatagagaac gcgtcgtaaa acttctcgtt gattttatga tgttcacgcg
      781 gcaggacgat gcccttctaa aagttattct tcgaccgcat caaatgagag ctgttgacaa
      841 aattgttgaa cgtgcaaagg atccagaaaa gcatcgggga cttgtatggc acacgcaggg
      901 ttctggcaaa acttacacaa tgattattgc agcgcagaag atcattgcaa atccgctctt
      961 cgaaaatccg acggttatca tgcttgtgga taggaacgaa cttgagagcc agctgtttgg
     1021 aaacatcgca gcgcttggca ttggaaacgt cgaggttacg gaaagcaaag agcatttgcg
     1081 gaatcttctt tccagcgatc ggcgcggttt gattgttacg atgattcata agttcgaggg
     1141 aataccggca aacatcaaca cgcgcaagaa tatttttgta ctggtggatg aagcgcaccg
     1201 aaccaccggc ggaaagctcg gtaattattt gatgggagcg cttccgaacg ccgcctacat
     1261 tggttttacc ggaacaccaa ttgacaaaac gcaatatggc aacggcacat ttgttacctt
     1321 tggcaaggat gatccaccac agggctatct tgacaaatac agcattgcag aatctatcga
     1381 agacggcaca acagtcccgc tctattatac tcttgctccg aacgaattgc gagtggagaa
     1441 ggaagcgctc gagaaggaat ttttgaatct tgcagaagct gaaggcatca gcgacatcga
     1501 agcgctcaat aaagtgttgg aaaaagcagt aacgctcaaa aatatgctca agaacaaaga
     1561 gcgagtggaa aaggttgcga gatatatttg cgaccattac aaaaattaca tcgaaccgat
     1621 gggctacaaa gcttttgtgg ttgcggtgga tcgggaagcc tgtgcgcttt acaaagagga
     1681 actcgacaaa tatctaccgc cggaatattc agaggtagtg tacagctccg ctttcaacga
     1741 ttcacctgaa ctggctaaat atcaactttc ggaaatagaa gaaaaaagga tacgaaaagc
     1801 ctttcgcaaa tcgaacgaac tgcccaaaat actgattgta accgaaaaac ttcttactgg
     1861 tttcgatgcg ccgattctct attgtatgta tctcgataaa ccgatgcggg atcatgtcct
     1921 gctgcaggca atctctcgag ttaatcgtcc gtatgaagat gatgagggaa gaaaaaagtc
     1981 ctccggcttt gtactcgatt ttgttggcat cttcgacaat ctcgaaaaag cgcttgcgtt
     2041 cgattcgaaa gacatcgaag gcattgtacg cgatattcag gttttgaaaa ataggtttga
     2101 aaacgaaatg aaactcggaa gagaaaaata tctcacgctt ataaaaggga aaacgcagga
     2161 taaagcggta gaggcggcgc tggagcattt ccgggatgaa gaggagagac aggaattcta
     2221 taagtatttc aaaaagttgt cggatatcta tgagatcatt tcaccggatg agtttttgcg
     2281 ctcttatctg gacgattacg aaacgctttt aagaatgtat aaaattgtaa gggaaaatta
     2341 tgagagaggg actctgatcg ataaaatgtt taccagaaaa actgctcttc ttgtacagaa
     2401 gcataccacg agcagcgata tcagaccgac gttggaagtt tgtgaaatca acgaaacgac
     2461 tttaagaaga atcgaagaaa gcaaagcctc tgaaacggaa aaagtattca atctcatcaa
     2521 gagcatcgag agaacagtcg aggagcaaag tggaaactcg ccatatcttt tttcgattgc
     2581 cgagaaagcg gaacttcttg taaagatgta caagatgagg caaaagaata cgcaggagac
     2641 gctggaagag ttaaagagga taataaaaga aatcaacgat gcaaggaaag aacagactga
     2701 aaagaatatg tcggctgaga atttcacaat ttactggatg ctgaagagcg aaggtttcaa
     2761 agatgccgaa gcgatggcaa atgagatgag gaatctgttc gagaaatatc cgcactggca
     2821 gaaaagcgaa aagtacgaga gagaaatcag gaccggtatt tatcagacta tcacgcgttc
     2881 aggcgtaaag gatgttaagg aaattcctga gattgtgaag aagataatga agacgctgaa
     2941 agcaaacagg gaagaatgat gacgccggaa aaattcaagg aaaatgtgct gacactggcg
     3001 aatgatgtgg gagtgaagcc aagagaaatc catattcaac cgatgaaacg caaatgggca
     3061 agctgctcga gcaaagggag acttacgttc aataagaaat tattgaaaga accagaagag
     3121 gttaggctga aggtgattct tcatgaattg ctgcacttaa aatatcccaa ccacggcaag
     3181 atgttcaaat tgttgatgga tacctatctt caaaaatctt tattgaaata atagaaaaat
     3241 tttaacaagc cgtagaggca cgttgcaacg cacctctacg gcataaaacc atcaatttcc
     3301 ttatgaatcc aaccattcag gaaatgttca ccaccgtacc gccaacttat cgaattctga
     3361 accaaatttt gactctcggg caagataacg tgtggcgcaa aagagccgcc cgactggcca
     3421 cttccgcagg cggaaggcga tggctggatg cctgtggcgg cacgggcgag atggctatcg
     3481 aactttcccg cctagcaaat tacggcacgt ctatatttgt agctgatttt tcccttccga
     3541 tgatgggcaa agccagggaa aagtccgagg cgaagcatat cattttcacc ttggcgaata
     3601 tgaaatgttt accttttcag tcggacactt tcgatgtcat tacaatatcc tttgccactc
     3661 gcaacctcag tataagtcag gataatcttc tccaatgctt aggagagttt caacgagttt
     3721 tgaaacccgg tggcttattt gtgaatcttg agaccagtca accccgatgg acgccgattc
     3781 gccgactatt tcacctttat gtcaaagcca ctgtgaaacc aatcggtcgc ttgatttcgg
     3841 gttcagaaaa cgcttattct tatctttccc attctattcg ccacttttac tctccggaag
     3901 aattggctga aatcatccgt caggctggtt tgcgcgagat cggtttcaag cggatgtttt
     3961 tgggcgcggc cgctattcat aaagccatca aataaatcag gcttaaaatc cagtatttga
     4021 aaaaatcgct acactaaact tctcaatgtc aaaaaaactc catttccatg atgaaaatcc
     4081 cttctaaatt caaagtatcg cggtcagcac tctaccagct ttcactttat cggttttatc
     4141 acctaaattt atatgtgtcg ccagtctgcc atagtcgaat taccatagca attaaacaaa
     4201 atgcttctcg ccagcgaggt caattatcag tttatcaact aaattataag gacttatatg
     4261 tacggcaaat tacaacagga tttgcagagc cagcttgaat ccataactaa acaggggctt
     4321 tataaatccg agcgcgtcat tgccagtccg caagcgcccc acttgcggct gactgatggt
     4381 cgggaattgc taaatttctg cgccaataat tatctgggat tgtcaaatca tccggttatt
     4441 attcaatccg cccagttagc tctcgagcgc tggggttttg ggctttcctc agtgcgtttt
     4501 atttgcggca ctcaggaaat acacaaggaa ttggagcggc ggttatcgga ttttctcggc
     4561 accgaggata cgattctgtt ctcgtcttgt tttgatgcta acggcggtgt tttcgagcca
     4621 ttgttcgatg agcaatgtgc gatcatctcc gacgaattga atcacgcttc aatcatagac
     4681 ggaatacggc tcagcaaggc tcagcgtttt cgttactccc atatgaatat agatgaactg
     4741 caaaaatgtc tggaaaattc tgctcaggca aaatatcgca ttattgtgag cgacggcgtt
     4801 ttctcaatgg acggtcacat cgcgcctctg ccacaaatct gtgaattggc tgaaaaatac
     4861 gatgcgctgg taatgataga cgattcgcac gccaccggct atctcgggca aacgggtcgc
     4921 ggcacagcgg aatactacgg cttgttggga aaaattgata taataacgac tactttcggc
     4981 aaagcgctgg gcggcgcttc tggcggttgc atttccgggc ggcgggaaat tgtggaattt
     5041 atgcgtcagc gttcgcgtcc ttaccttttt tcgaattccg tggcgccggc cgttattgga
     5101 gctactttaa aggctttgga aatcgtgcag gaaaatccgg acttgccacg ccgcacgatt
     5161 gaaaacgcac accattttcg ccagaaaatc gaagccgccg gtttcgacat tatccccggc
     5221 aataccgcca ttatcccgat tatgatttac gatgaacctc tcgccattaa aatcgccgat
     5281 cttctgcttg aggaaggcat ttatgttatt gggttcacct atccggtggt accgcgcggc
     5341 accgcccgca ttcgcgttca ggtttcggcg gctcacactc ccgagcagat tgatcgagcc
     5401 gtggcggctt ttacgaaagt aaggaaatct ctcaataagg gataaaaata gtgtttgaca
     5461 aagttataag ttttttgtag aattagcatc ggaagttgca atcccaattt tgaggattta
     5521 atttttaaca aaaaggagga ttaacgatgg ctctttcaaa gggaagtgtt aacaaggtca
     5581 tactggttgg tcgtcttggc cgcgatccgg aagtccgcta caccccgaac ggcgatgcag
     5641 ttgccgaggt ttccttagca acaactgaat cggtaaaaaa ccgtgacgga aaacgtgaag
     5701 accgcaccga gtggcacaat ctagtgattt ggcgcaatca ggcggaattt gccaaggaat
     5761 ggctcaaaaa gggacagctg gtctacgtcg aaggtcgtct ccaaacccgt caatgggaag
     5821 ataaagaagg tcagaagcgt agtaaaactg acattcaggt agaccaaatt actatgctag
     5881 gtggttccgg acgcggacgc gaaaaagaag ccgctgaacc agaaacgccg cccgagccat
     5941 ctccggatga tgaaattccc ttctaagatt atcttataat tcattgtgat cttaggataa
     6001 atctgacgcc gttgggcacc gatccggtcg gtctcagcat attaataggt aaaggaactt
     6061 ttaccgataa attcccgcat aatcgaggtc ggcggaattt cagtatcttc aactggcaga
     6121 aaatacgata gcatatccaa taagcgttgc gcttccggat agctgtgatg actttccggc
     6181 acatttttca gcatggtaac cgccagggtc ttcaaaacgc catgttcata gaccagcgat
     6241 gaattgaaca tgatatcggc gttgccctgg aaggtgaaaa tatatttatt ctcaccctcg
     6301 acaactttct tccacatttc gatagtttca gccgcactat acccgcgata tttataatcg
     6361 cgtacaatcc ggcgtaatag tcgggtgtca tgagtacgaa cacggttcat atgatcaaaa
     6421 ttcaagtgag taagggcact aacgtaaatt ttgaatttat aacgttcatt aacttcagga
     6481 gtcaagcccg gattaatgcc gtgaattcct tcgaaaatta atatcgtttg ggaatcaatt
     6541 ttcatgggct tggtggcggg agttttggtc ccggtaataa aattatatac cggcggtgta
     6601 atcgtctcgc cccgcaaaag tcgttgcaga tgatcgttca agagctcgag gtcaatcgca
     6661 tccagcactt cataattaat tcgcttgccc tgaagttttg ccagttcagc ccggtcacgg
     6721 aagtaattgt ccagtgacaa caaaaggggc ttgaatccca gaacccgtaa ctgaatagcg
     6781 aggcgcttgg caaaggtagt tttgccggaa gacgatggcc cagctacaaa tacaaaacgg
     6841 cgctcgcctt tatttgaaat ctggtcggca atgctggcta tttttttctc gtgtaatgct
     6901 tcatcaattt tgattaaatc cgaaatctga ttttcgacta tcgtacgatt gagatcggct
     6961 accgtactga tttctaaaat gcgtccccat tcctcagtct cctgaaaaat ttcatacagt
     7021 cgcgcctgat ccgtgaaagg tcgcagacga cgatcatctc cttcggtcgg acaacgcagg
     7081 acgaatcctt tttggtaggg tctaatttcg aatactttaa tcagtccggt acagggagcc
     7141 ggcggtgaga gtaaccagaa acggcgaccg ttaatttggt ataatgtaac aaaattggct
     7201 tcgctatatt tcagcaactc ttcagtatct tcttgcccga tttctttaaa ataccgacgg
     7261 gcgtcgttac aggaaattaa aagcggttcg atcgcataat cggcttctac cactttgata
     7321 aaagccgctg aaatttcttg aatgacctga ggcgtgcaaa aatcttcatt cacgaatcgg
     7381 caaaagacgc cgtcgcacat actatgcatc acgatcagac gctggtcggg gtacaaatcc
     7441 tcgaccacat aatttaaaat cagaatagcc gcgctttcat aagcccgccg ggatgactcg
     7501 tggaagtgtc gcagaaggct gattttagtc ggctcgttga caatttcgtt aaatgacgta
     7561 aaagtattgt tgcgaataac gatatacggt ttatatcggt tatgcggcag agcttgttta
     7621 acgatttcaa ttattttttg gccgggctga acagtaatcg ttcgcgtttc cccgagattc
     7681 acttctatca tttccataga ttggaaatta tcactaaaat actgaaaaat aaagtttggt
     7741 ttttgaagat agattaataa tttcagatta gtggtaaatg actgttcaat acttgccaaa
     7801 aataacatag gggaatcaaa atgaatactt cggaaactga aaatttgctg gctatgccga
     7861 catttcgaca cagacgtctt caaaactgga ttatcatcgg cttgttgtac tcctttttct
     7921 atatgtcccg ttataatttt tcagcaattg cgccgactct ccaagctttt ttcggctgga
     7981 ctaaaagcaa tttaggggtt ttcgaaacac ttatgccctt ggtttatggt ttggcggtcg
     8041 tcatcaatgg cccgctcgcc gataaaatcg gtggccgcaa ggctttcctg attggtgccc
     8101 tgggtgttgt aattatgaac gggctgtttg gatttttcca cattttggtc caaactccag
     8161 ccgtctgggc tgatcaagag attgtctccg aagccgtttt aaaatttgga ttctccaaat
     8221 cggcgcttct ctggacccta gctattatct gggcaattaa tggctatttt caatctttcg
     8281 gcgcattatc aatcgtaaaa atcaatgccc aatggttcca tatcagagag cggggcacat
     8341 tctctgcatt cttcggcgtg ctaatccgat ttggtctgat actggctttt tccgggacac
     8401 cattgatcgt aaaatttctg ccatggtatt gggctttttg gattccggca ttagtagtag
     8461 cactactttt cgtcctgacc attttctttg tacgagacac tcccgccgaa gctggctttc
     8521 cggaactcga tacgggcgat ggtgacgtta atcttgagga aaaagtcaag ttcaaagaag
     8581 tgcttaccaa agtgttcggc agtaaggtta tgtggactat tgccattggt tcgatgatga
     8641 taggttttgt acggcgcggc gtggtagatg cctggtggcc aatttatttc aaagaaatgc
     8701 agggtgttac cggagtagat ttcgcttttc aattaaccgc ctggggtatt gctattctgg
     8761 gaataatcgg tggttttgcc ttcggtattt cctccgatcg ggtctttggc ggccggcgtg
     8821 cgcccgtgat cgtaattggt ttctgcggta tgattgtaat tctcacccta ttctttttag
     8881 ctgacgtatg gcaatgggga ccgattgccg cggctatttt gttagcttgc ttgtcatttt
     8941 tcgttaacgg ttcccacggt atgattgccg gagcggctac gatggacttt ggtggtcgca
     9001 aagccgccgc gaccgccgtc ggattaattg acggtatgca gtatatcgcg ggagcattcg
     9061 tcggcgcgct ggtcggctgg gttactacca attggggctg gcaagcctgg aaaatctggc
     9121 caattccctt tgccattctg ggcgcactgg taatgtcttc tatctggcat actactccca
     9181 ctggaaaatc aacccattaa taatcaagga gcatttttat ggataatatt tttgacatta
     9241 tcattcttaa cggccgacca gcatccggca agtccgaggt cattgattat ctcaaaaaaa
     9301 ctccggtgga aatacgccgg cgccgttttc atattggcga gttggatgaa attgatgatt
     9361 ttccgatgct ctggacctgg ttcgaagaag acgccattct ggaaaaaata ttgaataaac
     9421 cgcgactgca ttccgacaaa gatggttatt ttttctggga atatctttgg catttactca
     9481 ttgagcgtat ctcaatggaa tacagtaaac gtctgcgcga caaacccaat tttcataata
     9541 actacacggc tcttattgaa ttttcacgcg gtagtgaaca cggcggttat cgagcggctt
     9601 atccgcattt atctgatgaa attttaaaac gggcggctat tttctacatt gatgtatcgt
     9661 tcgaagaatc actgcgtaaa aatcgccagc gctttaatcc cgctaagccg gatagcattt
     9721 tggaacatgg actgccggat gcgaagttaa cccgtctcta taaagattgc gattgggaag
     9781 aattccgcgg taatgaccct gaatttatta aaattaaggg acttaaagtg ccttatgttg
     9841 ttttacataa tgaaccggat atcacgactg cccgcgacga aaatctcggt cgggtattag
     9901 aagaactatt aaataaactc tggattctac gacagaaata aaatcctgga ttatatcgga
     9961 ttatatcggt aaaatgaatt tatgaccaaa tcgcctggga aaatctggca taagttgtac
    10021 agagaatatc gttcaatgct gaaaccgctc gcgatcgaaa gttggccgga tatgtttttc
    10081 tatcgaccgc tggctttcgg tttggtgaag accattatac ccttgccgat tacgccgaat
    10141 atgatttcgc tttcggcgat tatcatcggt ttaagcggcg gctactgtct agcgatcggc
    10201 actccgcgga tctgccaaat cgccggtttg ctgtttatgg ccaatgtcat tctggattgc
    10261 gcggacggta tgttggcgcg ttataaacac aatggaacgc cgctgggctt tatagttgat
    10321 ggtctggttg actattgcaa cggagccgcg attttcatag gtatgaccgt cggtttcagt
    10381 cgtactgatt cacaatttgg attctcgctc tgggtgctta ttccattgag tctcttttcg
    10441 tggatcgctc attcgatggc agtcgatcac gcccggcggg aatttcagcg caatgctctc
    10501 gaacagtgtc cgtcattggc cggaggtcgg caaaattttc agaattatcg tgaacagcta
    10561 agccgctcga agggtcactg gtctgataaa ttgctggcta gcctttacct attctattgc
    10621 aatgtccaga aaccgaatgc caaatttagc cgcacctacc cggctgacag atattatcgc
    10681 tacaacaatt gtgttatgcg cggctggcta ttgatcgatc cttctaccca tatgaccatt
    10741 gcaattctgt cggcattatt atataatccg ttgatatttt tctggtacac gatcgtaatt
    10801 gctaatatca ttatgttaat gatgtatctg attcagtatt ttgtaaacca aaaagtagga
    10861 gtcttgccgg tctgatgaaa gctctcgtca ctggcgcaac cggatttatt ggtcagcatc
    10921 ttatccgacg tttacttgca gaaaattgcg aggtgcgcgc tctttgccgc tccaccagta
    10981 tcaagccgga tgaattcgcg caaatcgtcg aatggcaaat aggtgacatt actgctccgg
    11041 aatcgctggc ttcagccgtc tcagattgtg acttagtctt tcatcttgcc ggtgctatca
    11101 aagcccaaga tgatgccact ttcacccgga ttaacgtcaa cggcaccgtc aatcttttga
    11161 aagccttgaa gcaatacggc aagaaagagg tgcggttcat tttcgtcagt tcactttccg
    11221 ccagtggacc gtctgagcct tggtcaccga aaaatgagtc tgagccctgt tatccagttt
    11281 cagcttatgg acagagcaaa ctcgaagccg aaatcgaagt tctaaagcag aaaaacagaa
    11341 tttggtgcac aattattcgt ccggctgtgg tttatggacc cggcgataaa gaatctctgg
    11401 ctttcttcaa aatcgctaaa tcgcgtttta atccgcatct cggcttcgaa aaacgttacg
    11461 ttggtatgat ctatgtcggc gatctggtgg atttgttatg gaaggcggct ttgagcaacc
    11521 gtctatccgg cgaaatatat ttcgcctgcg atgaacaatc cgacggctat gagtggaatc
    11581 agattattgc tacggctggt aaagttcttg gctatcacac attaccaatt ttagtgccgc
    11641 aatttatctt accgatgatc gtagttccca cccagattat tgcaaaaatg actggaaaaa
    11701 ttggtagtct taatcgcgac aaatatcgcg aaatgaatca atcccattgg acctgtacat
    11761 ccgccaaagc ccaaaaattg cttgatttta agcctcaggt cgatctttcc gaaggtttta
    11821 aacgcacagc tcaatggtac cgagagcaaa attggatcta acacccgaat cgcccactcc
    11881 cttaatcaac aactacaaaa agggagtatt tccagccgat tgcctggtat tcggttttac
    11941 tgtgttaatg ctattaatcg gaattaccaa ttattctcgg cttggcaagc catatacctg
    12001 gattagtacc gactgtggaa taattattgt gctattaatg ttgattttca aggaaacgca
    12061 cgaagagcgg cgattttttt actggcttca ttttttctgg ccgattatat ccctggcatt
    12121 tctatatacg caatgtacct cctgggataa tctcattttc cccattactt tcgatcccgt
    12181 acttataaaa tgggatctca ggctgttcgg ttcggctttg aacaaagtaa tggcttcatc
    12241 ggtcgatagt ctgtggctcg atgaaataat gcatttcttt tatttttcat attacttagt
    12301 catcttttta acagttgttt ggatgcttgg gcggcgtcag cctcaggctt atgaattgat
    12361 tttcagtctg acgcttatga tgtatttcca ttttatattc tttatgattt tcccaagcga
    12421 tggaccgctg gcggaccgta acgatctctt taatcagggt gtggttttta ttcccctaat
    12481 ggattttatt tatggagtgt ccgaacaaaa tggtggcggt gcttttccca gcacgcatgt
    12541 ttccgccgcg gttgtggttg ccatttacac ttttaaaaat tcacccaagc tgcgcattcc
    12601 aatcgggggc ttgtgtctcg gaattataat ctccaccgta tattgcagtt accattatgc
    12661 catagatgcg gccgccggaa ttatcaccgg cacacttttt tattttctcg gtaaatatat
    12721 ctataatcac cgttttcagc ccgtcaattt atcgctattt atgccccaaa aaagcatcta
    12781 aaacctatat ataagggagt taccaatgaa atcaaaagct gtcggattat tatggctatc
    12841 tctaatcata atggtggaaa taggattcac tgctaagcgc tctatgcaga ttgatgactt
    12901 actgaattgt caccgactgg cagaacccca gtattcaccc gacggacaat ggatcgcctt
    12961 caccgtacag accatagata tcgaagccaa taaagggcaa aaaacactgt ggcgtatccg
    13021 ccccgatggc caaaatttac aacagattcc tacccattct ggcaatagct ggtcgccact
    13081 ctggtcaccg gatagtaaaa gtttagcatt tttatccgat agcggcggac aggttcaggt
    13141 ctggaaactg gcgttggata ataatcatgt tcaacaaatt actgactttt atggcggggt
    13201 agaaagtttc tgctggtcgc acaatggtca aaagattgct ttctcggccc gtgtctatcc
    13261 ggatttgact gacttcgatg agatgaaaaa acgcgacgcc acccggcaag ccagtctaat
    13321 caaagcccgc gtctatgaag aattaatgtt tcgtcattgg gacgagtggt gggattataa
    13381 acgcagtcac atcttcgtgc ttgatttgaa taacggtagt ttaacagacg ttacgcctgg
    13441 cgattttgat gccccaccca tagcgctggg tgaaggctac tgtttttctc ccgatgacca
    13501 aacaatcgtt tttaatagta atcacgatcc ggtcatcgcc attagcacca ataacgacat
    13561 ctggtcagcc tcaattgcta atccgcgcga accaaaatta attaccaccg cccaggagta
    13621 ccgtgacttc cgcggcaatg attacgctcc cagattttca ccggacggca aatatctaag
    13681 cttcctttcg atggcacgtg ccggtttcga agccgataaa tccgatttgt tcttgtatga
    13741 aatgactact ggcaaattta tcaatctgac cgaaaaaaac gatatccgga ttcaagccta
    13801 cgaatggttg cccgatagtc gtggtttgct cttactgata gaagaacgtg gtcggctaac
    13861 cttgaaaacc ctgaatatta aatcaggtca gataaagacc ctggtcaaag aaggctttaa
    13921 cagcgcagta acgctggctc ctgacgggcg gtcttgtgct tttttacagt cgcggactac
    13981 cagccccgat gaacttttcg tatattcttt cgccgataac aaaattcggc aaattaccga
    14041 cttcaactct ccgctaaggt ctcaaatcga aatgaatgac gttgaggatt ttgagttcaa
    14101 aagcagtgac ggagcgatgg ttcatggctt tttgatcacg ccgcctttct ttgatgcccg
    14161 ggagaaatat ccggctgttt ttctggtcca cggtgggcca cagagcgcct gggaggataa
    14221 ttggcattac cgttggaatc cgcaactatg gtcggctcaa ggttatgtaa ttataatgat
    14281 taatccgcgc gggagtattg gctatggtca aaaatttacc gacgcaatta gtctggattg
    14341 gggtggccag gtttttgatg acctcgtcgt tggacaaaaa tatgtaatag accacttcaa
    14401 gttcatagat gccgataaat tagccgcggc gggagcttct tacggcggtt atatgatgaa
    14461 ctggatggag ggtcatatgg aggcgttttc gtatcctttc aaaaccctcg tcaatcacga
    14521 cggttcattc aatttatatg cgatgtatct tacaactgag gagctctggt ttcccgaatg
    14581 ggaatatggt ggagttttct gggaaaatcg agatcaatac gaaaaattct cctgcgacaa
    14641 ttacataata aatttcaaaa ctcctatgtt gattattcac ggtgaaaagg attatcgctt
    14701 agatccaagt cagggaataa tggctttcac tgccttgcgg cgcttgaata tacctgccaa
    14761 gctggtactt tttcccgatg aaggacattt cgtgttacag ccacaaaaca gccgtttttg
    14821 gcatcagaca atttttagct ggctcgagaa atatttacgc taaatcacaa gttcttcggg
    14881 atacgccgat catagtattt taacttatta gtgcgtaatt tgtcttataa cgcagattca
    14941 ttatcattat taatatggaa cattcaatta aaccagtcat tttagcagta aaaacaggat
    15001 taaaaacatg aataccattt tctcttctac ttatgatatc ggcataatgg gatgggtaat
    15061 tattctgatc ttgattgggc tttctatcat cgcctggggt attattattg agaaaatgat
    15121 tctcttcaga cgcaccgaaa aacaaaataa aaaatttctg gctatttatc agtcgtattc
    15181 cgactggagc gcggtcgcaa gggaaactca gcatctcaag tccggtttac ttctcaatct
    15241 ttttcgaaaa atatacgctg aagcccgcaa agatgccgag ctcaatccag gcaacgtggt
    15301 tactgagaac atagataccg atgccagtcg catatcgtcg ctcgagcttt ataaagccct
    15361 ggtggattcc gcaattagca ccgaacttat accttatgaa aaaagattga tatttttgtc
    15421 ttctacggtt agtgccagtc cgtttctggg attattcggt accgtatggg gagtcatgca
    15481 agctttcttg agcattggag taaaaggctc agcggcgatt acagccatag gtcccgggat
    15541 tgctgaggca ttaattacga ctgttgtggg tttggcagtc gctattccgg tactttttgc
    15601 ctataactta atcgtggata aaatccgaaa atacgagaat caattacaaa ttctcgccaa
    15661 tgatatgatt caaaagatca tccgggaaaa tttttatggt ggatcgcaga tataagccgc
    15721 tgaccgaaat caacattact aatttagttg atgtcacttt aacagtttta atcattttta
    15781 tgattacgac acccttaatg tatagtggta tagaggttga tttgcctgct accagacgcg
    15841 aaatcactac tgtccaagaa ggcatcctgg ttaattatag tcttgatggg caaattttta
    15901 ttgatgggca accggtcatt gaaaatgaat ttgaaaaaac tttagtcaaa cgctgggtac
    15961 aagtcggtaa gaagccggtt ttattaagtg ccgatcggaa aattgaatat ggtaaaatta
    16021 tcaagctgat tgaccggata aaagcttgcg gcgtgaccga attgggatta atagtcgaaa
    16081 ccgaacagga taagaagtga attacacttt tgccatatcc ttaattttac acttgacgtt
    16141 ttttgccgtc gtaagtgtct tggctggtcg ccaccagcct cgcgttatgg ccatcccgga
    16201 tcagaacata cgctgggtca atatcgctcc accggtcgtt caaactgctg aagctccaat
    16261 tcaggtaaaa ccagccactt ccgttccagt tcctaaaccc aaagtagaaa aagctgctac
    16321 tgttcttgcg gaaaaaccac aaaagcccca agagaccaaa ccgttacctg aagaaacgat
    16381 cgcctcgtcc acgacctcac cggagctacc cgtcgagccg gaggctacat ctcagcccgc
    16441 gtctggcggt attcccggtg caaccggttt aagggtagat gatcccgact tcaattttat
    16501 ctattatctt aacattatcc gcaaccggat tcaggaacat tggcggcctc cctattcttc
    16561 agcagattct ccctacagtc agcaagttat gattgccttt aaaatcaccc gttccggaaa
    16621 aatttccgat gtacgaatcg aacaatcttc cggtaatttt ctctttgatc aggcggctct
    16681 gcgcgcgctt tacgaaacgg ctactttacc acccttaccg agtgaatatg gcggaaaaga
    16741 gttaaatgtt catatcgaat ttgaaacttt gaaataaacg tggtatgaaa aaaaatctat
    16801 cattgatttg cctaatatcc ttcttgaacg tcactggaag tagtcaaaca gatgtgttcc
    16861 ttaaaattca atcggacgag acgcgcaaaa ttaatgtcgc tatcgctgaa ttcggtacta
    16921 atcaaaatcc agagttggct attaatttac gccagattat tattgatgat ttgaattatt
    16981 cgggatttct caatgttgtt gatccgctga tcagccctgc cggagattta atctttgata
    17041 attcagaggt aattatcacc ggtgatgtta ctcacaagag tagttatttc aattttgtat
    17101 tcgggcttac cgaatctgtc accggtcgga aactaatgac caagtctttg cgctcttacc
    17161 cctttgctct gcgagttctc gcccataccg tttcggacga aatcgtgtac caattatgcg
    17221 gtgaatatgg caatgcctgc actaaaattg cttatgttac cgaacaaaaa gggggcaagg
    17281 agttagccct tatggattgg gatggccagc gcgcgactct cctgacccga aatcgttcaa
    17341 ttaatttact gccatgctgg tcgaacgatg gagaatcatt aatttttact acatacgctt
    17401 ttggtggcgg tcccattctg gcgcgttata tattcaaaga ccagcaaatt gaacgattca
    17461 tcgcgcgcgc cggcacattt acttcgccgg agtattctcc caacggtaaa aaagttgcct
    17521 ttgtccttac tcacgagggc aatgccgaca tttacactgc tgaccgtaac ggtcaaaatc
    17581 tccagcgcct gaccagcaat ccggctattg atacagcccc gtcgtggtca cctaacagtc
    17641 gcgaaattgt attcacttcg gaccgtagcg gttctcccca aatttatatt atggatgccg
    17701 aaggtggcaa tgtccgtcgg ctgacttatg agaacaacta taattcctca cctaaatggt
    17761 caccgaaagg cgatcaaatt gttttcgtct ctaatcaatc ccgcggtttt caaatctatc
    17821 tcattgatgt taccggagaa aatctaatct gcctgactga taataccggt agttacgaaa
    17881 atcctacctg gtcgcctaat ggtagccatc tggtttgcgc ctccaaccgg agtggacgct
    17941 gggaaatcta tagcatgttg cgcgacggat cactattacg ccaattaact ttcaccggtg
    18001 gcaataccgg tccagcctgg tcgccgcgct tgaaaccatt taactagaaa atcatttcag
    18061 ggtgtatgag tataacacta attaacaaaa agaaaggagc aaccaaaatg aaaaaacttc
    18121 tcgttttttt aattccttta ctctgtattg tattaatgtt caatacttgc agtaaaaaga
    18181 ccgcgggggt gcaagaagac aagggtgaga ctccagccaa agtcaccccg gccccggaac
    18241 agcctaaacc tgtccaaaag ccggatactc tcgcccaagc accagcccct gtagtcaccc
    18301 caccgcctgc cgaacccaaa cctgtcatag cggacacaca aaaggtggcc gccattgctg
    18361 aaccttccct aaaagatgaa ctcgcgcaat gcaatacggt ctatttcgat tacgaccgtt
    18421 atgacttgcg accggatgcc atcgccactc tcgaaaaagc cgccaaagtg cttaagcagt
    18481 atccgaacgc cattatcact atcgaaggtc actgcgacga aagaggcacc gtagagtata
    18541 acctggcact cggcgagcgt cgagctcacg ccgtaaaaac ttatctcatc aattatggaa
    18601 taaaaccgga caatctaatt acgataagct atggcaaaga aagaccgatc gatccgggcc
    18661 ataatgaaga ggcttgggct aaaaaccgcc gagcggtatt ggtcacttcg gactcaaaat
    18721 aaattctcgg cgctttcatc aaccaaggta gctatgaact ttaaaccaat tcgtccaatc
    18781 atttatggtt tgttcgtgat cagcttgacg gcttgcgcca ctcaaaaaga tcttttgaaa
    18841 attcaagagg atttatattt tttacgcaac cagattcaat ccgttaaaca gactactgaa
    18901 gaaaataaac aattgttaag tcaggttcct actattgcgg ttcaatgcaa tcaaatattg
    18961 atagagctag attctctgga aaaatccctt gcggaattac aagatgccaa tgaccgaacc
    19021 cgagctgata tcaatacccg actatccagc cttaagcaag atgcggcgat tatcagcagt
    19081 aaattggacg acgcaagcta ccgctccagt cagctgatcg gcaaggtcga atctttgacg
    19141 ggcaaaatga ccaatatttc ggaaaaaata gagagccgcg ccgccactcc caactcggct
    19201 gagccggctc ccaccgaaat tttcaacaac gcttatcgag atatgactaa aggcaacatc
    19261 gaactggcta ttcagggctt ccagactttc ctcaaacttt atccggaaaa ggaattagcc
    19321 gattattgcc aatattatct tgccgaaatc gcctatcaac aggaagacta cgccacagct
    19381 gcgaccgaat acaagctatt gattaataac tatccccgca gtcagaaaat cactaacgcc
    19441 cattacaaac tgggattatg ctatttaaaa ctaaacaact tggagcaggc acgcacccag
    19501 ttcgaaacag ttattcggga ataccccaat actgaagaag ccttacttgc ccgtaatcag
    19561 ctgcagaagc tggaacaaca ataatccgga aggaaaatcc aaaagttatc cgcactgtcc
    19621 cagacggttg cggataactt ttatttctgc gcgcgcttct tttatcagat cattaatcat
    19681 cttttgcatc gggacaatct cgttaattag cccaacactc tgaccggcca ttaacgagcc
    19741 ataatctaca tcaccctcca ccaccgcccg acgcaatgcg ccaacccaaa aattctcaac
    19801 ctcatattga gccttctccc gcgtaatctt accggcagcg atttcctgta gtaaacgcat
    19861 ctggagcttc ccgaactcat ccgttccttt gttcttgata gctcgtacgg caacgaccgg
    19921 caatttggaa tcatattgtg gagtagcaat agcctggcgc gctttggctt tcagtaatgc
    19981 cgccttaaaa ttcggatgaa cccgacattc ttcagttaac acaaagcggg ttcccatttg
    20041 gaccccagcc gcacccatta gaagcaagtg agcgatcatc ctcccatcag cgatcccacc
    20101 accgacgaaa atcggcaaac cttttacttt aaacaagact tgctgaagta aaacaatcaa
    20161 tgaaacataa ccgatatgtc caccggcttc actgccttcc aaaatcagag catctatgcc
    20221 atattcaatc tgttggcgcg ctatgctttc ttcgggagcg aacgatagcg ttcgtttgcc
    20281 gcgggttttc atcccctcaa tatcctgacg gcgaggaata ccaccggcaa aaactacaaa
    20341 tgggacgttc ttgctttgta aaatttcata atgtattcga aaattaggcc cgatcgtgat
    20401 taggttgacc gcaaacggct tctttacacc agcaatacag cggtcaactt cttgctcgaa
    20461 taaatcagga ggcaagttgc cgcctgccag tactccgaaa gccccggttt cactgaccgt
    20521 ttttaccaga ttataatcgc tcacccaagt catagcgccg caaatgatag gatatttgac
    20581 gcccaaaaat tcagtgcccg gtttgattaa tttttcaaaa ctcattattt caccttttgt
    20641 taaaattaaa tttcaaacgc aggaaaattt acaaaatctg agggatttaa gtttctgcgc
    20701 cgcgggaatt ttcgttaatt gattgataaa ttatagggcg ctcattagaa gagctaaaag
    20761 tgtcgagttc aatttttccg ctggctcgtc ggaacgcgaa agtaaaacta tcggcacccg
    20821 cccacctact accactccgc cgacttcagc cttagccagc aacattaaaa acttgataat
    20881 agcattgccg caggtaatac ccggtaccag tataatatct gtatcaccgg caatgcgact
    20941 atgtaaacct ttacgttcgg cggctctcgc tgagagcgcg acatcagccg ctactgggcc
    21001 ttctacaatt gccgaaccaa attcaccccg ctcagccatt tcttgtaaag tcattgcatc
    21061 tactgtttcc ggaattttgg gatttacctt ctcgattggg gctaaaactg cgatttttgg
    21121 tctggtgatt tctagctttt tggcgaaaat aatggcattg ttaattatag cttttttctt
    21181 ctcgagatcc ggcgctatgt taactccgcc gtcggtgacc atgagtaatt tatgatagtt
    21241 ggaaacggca acgaccgtga tatgacttaa aagatcccca ctgcgcaagc ctttttcttt
    21301 atccaatacg gctttcatta aagctggcgt cgtaatatgt cccttcatca ggatctcggc
    21361 ttttcctaaa tgcaccagtt cgaccgcccg tgtggctcct tcttcttcac taaatgctga
    21421 aattatttca tcttctttta tgggataacc aacctgttca gcatacgact taattttctt
    21481 cggattgccg gttaaaataa cccgggcgat atcgtttgcc gataggactt taatggcttc
    21541 cattacactt ttatcttcag ctaatgcaac tgctatcgtc ggtctacgag catttttttt
    21601 aactttttca aatatttcgg aataggtctt tagcataagt tattctttga cattaatctc
    21661 ttcataaatc agaatttcct cattcccatc gagggcgcgc cagccgcctt cagccagtgc
    21721 ttccatttca ttttcacccg ggaaaacaat caacggggca atgaatccca ccccggcgcg
    21781 gatcatctcg acggtttcag caccacgcgc ctgtccaccg gtgacgatta ttgcgtccac
    21841 atttcccttc aaaacagtcg ccatcgcgcc aatctctttg caaacctggt aaatcatcgc
    21901 cctgaatatc aatgccgccc gctgatctcc cgtctctgct cgttgccaga cttctcgggc
    21961 atcgtttgta ccgagataag cgaccagacc actttttttg gtcattttta ttttcatttc
    22021 ttctttagtg tatttccctg aatacgccaa attcagaata ccttccaggg gcaaagctcc
    22081 ggctctttcc ggcgtaaaag gccccatccc aagcaaagca ttgttgcaat caatcagacg
    22141 accacctcta accgccgcta tgctgacacc gcctcccaga tgggcgacca caaaattcaa
    22201 ttcactaggc aggcgaccca gttgcgcgct ggctttgcgc acacaagccc gaatgttcaa
    22261 agtgtgttgt cggctcactc tttcaatttc aggaatacct gaaatgcgcg cctccggaat
    22321 catttcgtca accgtaactg gatccaccac aaagctggaa atatgatgtt gattagcgat
    22381 cacttcggca atcggagccc ctaaattact ggcatgctcc ccatatttac agctgattgc
    22441 atcggtaatc attcccgggg taattttata cacacctgcc cgcagaggtt tgagaagacc
    22501 gccccgaccg accactgctt tcaggcaata atcaaataac cagggcttaa ttgtattagt
    22561 aacaatctcg atgcgtaagg gtaattgctc cgcgatcgag tgaatccgac ttatgggagt
    22621 accgacatgg ctgatatttt cgaattggag tcgcctgctc cgatcgaata gtgcgatttt
    22681 ggttgacgtt gaaccaggat ttaccaccaa gactgtttct atcatactgg gctttgcttc
    22741 ttatttgtat ttgtcagaca tctctaaccc attgccagac aaatatgatg ccattgatta
    22801 ctttatcatc gtgaagtaca ttttaaaaga gacagaaggg accgccgtta tttctaaatt
    22861 ttaggaatta tctaattttt tttcccataa agtgaaatca atcggcgagc aggagtatag
    22921 aaataatctt tcgtatatct tactattctt ttaaatttta aacacaatga atcaaaacgt
    22981 ctattttatc gccgacattc acctgcactt aaacgatagt caggaagaaa aggtaaagcg
    23041 cgaacgcctt tttgcgtttc tggcagaagt ccgacgtcac caaggttcat tatatattct
    23101 tggtgatatg ttcgattttt ggtttgaata tcgttacgtg attccgggcg tattcttccc
    23161 ggtgttatgc gagttacgcg aaacggtttt agccggatgc gaggtgcact ttcttggtgg
    23221 caatcatgat tattgggcgg ggcaattttt cacggacgtg cttggcatta cggttcacta
    23281 taaaccgatt gacacgataa ttgccgggaa aagcttccat ctgacccatg ctgacggtat
    23341 tcttcataat gaccgcggat accggttgat gcggtttttt ttcaggaata agaccatcat
    23401 tcgcctattt cgctatcttc acccagattg cgccatccgc ttggctaccc atttatctgg
    23461 gagaagccgc cgtctgactc tgcgcagtcc ggctcaggaa gaatccgacc gccaagaaat
    23521 cattgcctat ggcaagaaaa aattggaggc aggcgctgaa tatgtcatca ccggccattt
    23581 acatttgccg actgagtatc ataccgagag aggagttgtg gtgaatctgg gtgactggat
    23641 gaaatattat tccttcgcat tctttgatgg caaatcactg cgcctatgtt attggcctct
    23701 cagcacctct gattccaccg gtaaaaaatc agaattttta cttgactaag aaataaagat
    23761 gttctaaaat catttgggat aaataaagaa cggaagaaac acatgaaagc aaaaaatatc
    23821 ataattatca tgttggttct cggcggctta atgtcctgtg ctcctgagac cgccaccatt
    23881 gatccccgcc aagtcgaaaa actgcgggtt gattacaatc ggggaaaaat ccagaagctt
    23941 gatgacttaa tcgcaatata tcaagaccaa gcgcaaccta ttgagactcg catcgccgcc
    24001 gtgaatgccc tggctgaaac tcaacatccg gatgccatta aagctatgca cgattttatg
    24061 gatcagtcgg tcggcctcaa ttatgcttta ctgaatgcca cggcaaccgc tttgacgaag
    24121 cacgccacac cggaaaacgt tgcggctatg gtggatggga ttgtctccgc ccagaagaaa
    24181 tacattaact tccggactgg cgtaatggag aaaatggaag atctgaacgt tgctctacag
    24241 gtggaagcat tgcttaagct gtatcaatcc gaaaaggaaa actacatttt tatgcaagaa
    24301 tcgctcacta agttgctggg ttcgattccc gacgaccgcg tcattcccat cctgatcaat
    24361 atcgccaaag atgaaagcgt caacatttcg acgcgctcac tggcgatgga aattctcggc
    24421 aagaaacgtc atccactaat cacagagacc ttcattgaaa tgcttggcga cccggaacag
    24481 caattggcat tgcgcgaatt tgccattaaa tccattgaag acgtcaaaga gtcgcgggtt
    24541 attttagcgt tgttagaaac tctaaaccag agtcgggagg aacacttggt gctgttggac
    24601 gctcttaccc gagctatggg cgattattct gacccagccg ttattccagc tttggtcgaa
    24661 atagcgaaaa acaccgatta cccggtggct actcgccgca atgcactatc agctctggtc
    24721 aagttccaag acatcgaggc ttttaagcaa ctcctgccga ttatggaaag cccagaaggg
    24781 tatgtgctct atgacgaaat ggctcagata gccaccgcaa ttggtcagcc ggaagttctc
    24841 aatcaactca gactcgccgc cttaaaagcc cagctaaaag ctcgggaggc tcaatgaaaa
    24901 aacagatcct atatttaatc ccaatgattt gcgccgtatt tgcagtttct tccttcgctc
    24961 agtcaaccag tccgaaaagc gctactactg ataaattctt cgaggtggac caaagtattg
    25021 aggaactacg ccaggaaatc gccgaaattc gccggcaaat ccgcgacatc gaactgcgca
    25081 cttccacccc ggaaatccgg aaggaaatca accggctaat acccgttcct gacatcactc
    25141 atgaaatcat cttgcataac ggtaccgtcg tgcagggcaa aatcattcat gaaactattg
    25201 ataaaatcgt cattcagact aacatcggtc agctgaccat ttcgaagaat gatattcacg
    25261 tctttcggga agctgaaaaa ccacatgcta agtgtgtatt agtcgggccg ataacgccaa
    25321 aaattttcga agacaagacc acctataccg gaaaaattaa aaacgagggg gcgcggcgag
    25381 ccgatttccc gcagataaaa atacaattat tcgatgaggc tactaatcta atcgccgccg
    25441 attcgattat tatcgccggt aattatcata tgtttttgag cggagtccaa accgacgcta
    25501 cgattgagcc gggacagact ttcgatttcg aaatcacgat ttcatatccc gccaatacta
    25561 aagtcagcta ctttattccc aaaattacct ggcaagaatt tgattgagat cgcttagtca
    25621 ggcgttcttc tcgaccgtct tctgctataa aatttcacaa ctcaatacgc tttttattac
    25681 aattaaatat ttag
//
LOCUS       DAOMJU010000024        25194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1825_length_25194_cov_25.938025, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000024 DAOMJU010000000
VERSION     DAOMJU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_25194_cov_25.938025"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..562
                     /gene="tnpA"
                     /locus_tag="PLO58_03755"
     CDS             110..562
                     /gene="tnpA"
                     /locus_tag="PLO58_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015133970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HQO73939.1"
                     /translation="MSEHIIKSHNKTLLLYHLVFPVKYRCGVIKEELGETIKAIGLEI
                     SERYEIHFVEIGADEDHVHYMVQSVPKLSVTEIVTTIKSITAREIFERQPEIRQQLWG
                     GHFWTSGYYANTVGRYGNEFLIQQYVKEQGKKYDRIYRGQLTMFEGLS"
     gene            973..2364
                     /gene="dnaA"
                     /locus_tag="PLO58_03760"
     CDS             973..2364
                     /gene="dnaA"
                     /locus_tag="PLO58_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQO73940.1"
                     /translation="MPNSLICSDVWNSCLEYVRKKIPQQTFETWFAPMKAISLADDTL
                     ILQVPNKFFYDWVDNHYREMILKALKEVTGNGFNVSYTILLGEEVEQPAGTHSVSKRG
                     SGVTSVAEPDFIAGAREQGTRLNERYVFDTFVEGASNQFARAAAMAVASAPGRTSFNP
                     LVIYGGTGLGKTHLLQAIGNEALNNGRAKKVVYVSSDTFTLDFIAAIQNNKTTEFSRS
                     YRSIDMLLLDDIQFFQNKEQTQEQFFHIFNDLYQRHKQIVLTTDKPPMELRGLQERLL
                     SRFQSSLTVDIQPPDLETRIAILQKKANDDGLDIPYEVIEYLSANIRSNIRELEGALI
                     RLLAYSSLLQVDIDINLTQRVMREILGTRHSSVLSIESVQSTVSTVYKINLDSMVGKG
                     RTKEVAEARMIAMYLTREFTDLSLKTIGLYFGGRDHSTVVHACRWVEERLQSDSVFAR
                     QINDLKAKLSQNI"
     gene            complement(2371..3915)
                     /locus_tag="PLO58_03765"
     CDS             complement(2371..3915)
                     /locus_tag="PLO58_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQO73941.1"
                     /translation="MTKFSLVKSAAVLGIDAYIVDVECNLSNAQLPAFITVGLPEGAV
                     RESKERVWSAIKNLGYYVPSNKIVVNLAPADIKKEGSSFDLPIAIGILTAADLIEKHK
                     VSDYIFLGELSLDGMLREIKGALPISVSVRNSGIKGIVLPEQNAREAAITGDLEIIGL
                     KSLQEVIDFLENRTHPEPTHVDVRALFEEHRQYQIDFSEVKGQENVKRALEVAAAGGH
                     NIIMIGPPGSGKTMLAKRIPTILPNLTLEEALETTKIHSVAGLVPSGQGIVATRPFRS
                     PHHTISDAALVGGGKFPRPGEVSLSHHGVLFLDELPEFKKNVLEVMRQPLENGYVTIS
                     RALLSLTYPASFMLVAAMNPCPCGYATDPHHQCTCTPQQIQKYMARISGPLLDRIDIH
                     IEVPAVKFSDLSAKAPGEPSEIIRERVQAARLKQTARFAGIKGVFANAHMESRLIRRF
                     CQIDQTCEDLLRNAINKLGLSARAYDRILKVSRTIADLSNSEKILPEHISEAIQYRSL
                     DRHLWL"
     gene            complement(3982..5082)
                     /gene="plsX"
                     /locus_tag="PLO58_03770"
     CDS             complement(3982..5082)
                     /gene="plsX"
                     /locus_tag="PLO58_03770"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQO73942.1"
                     /translation="MNNLDNKQPSAVIAVDAMGGDFAPDEVVLGAARASVETPIKIIL
                     VGDQSKIEPILEQTEHNPQQLEIVHTDQYITNDDKPKFILSKKPRASMLIASQLCGAD
                     QAHGIVSAGNTGAYILSAIKYIPRIEGVHKTAIASAYPTRNEQGRSDPFALLLDVGAN
                     VHCTADDLVQFAIMGKIYASAVKGIENPTVGLLNIGKEPYKGGDTLTDAFNMLNSIQD
                     MKFIGNIEGNEILKGIADIIVAEGYVGNIVMKTIEGFGETISHIGRYAFKHRFIWMLG
                     LLALSSGIKQLKKVADYSEYGGAPLLGFRKIVIKAHGRSRAKAITNAIIVAAKTYHDN
                     VCEKMAREIAQYEKQFQRKNVMNCDWKLNFDQ"
     gene            complement(5079..6044)
                     /locus_tag="PLO58_03775"
     CDS             complement(5079..6044)
                     /locus_tag="PLO58_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQO73943.1"
                     /translation="MKIFTYIEPENGNISKNSAGCLTLGRQLADLANGSLTAIVPAQE
                     SSTMQNECGHYGADRVAFTKNFAKYIPEVTASDVANILLEENGDILILPDRPAIRDIA
                     AIACETVSGSIAVDCLQVNLDSEQNPIVRRAMYGGKALADLKLIKKPVFITIRANIIP
                     PQENIRSAEIINSPSQLSAVTPKIKINEIRPVASKRPALTDAKIIVAGGRGIGAPENF
                     HLIEELADALGAAVGASRSVVDAGWVPQTFQVGQTGKTVSPDLYIACGISGAMQHIAG
                     ISSAKYIVAINNDPDAPIFKIANYGIVANLFAIIPEMIKHLKNIK"
     gene            complement(6057..6809)
                     /locus_tag="PLO58_03780"
     CDS             complement(6057..6809)
                     /locus_tag="PLO58_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQO73944.1"
                     /translation="MKIVVCVKVVPVSDSIITIVPEKTAIDLVNIKFDLNPYDEFAIE
                     AAVLLKEKNGGEVIAISLGPERDNISLKRVLALGADRMIHLVTADKALSDPLATAKAL
                     ATEIKPLSPDLIFCGKLSIDTGNGLVGPLVACLLEYPFLTGICELQVGTDRLTAAKDT
                     EEGRIIYESSLPCLLTTEKGLNKPRYPSLKDIMLSRKKEIITKNVNLTEKASDIIQLS
                     EPAPRPAGTILGQNVDAVPELIRRLREEAKVL"
     gene            complement(6821..8167)
                     /locus_tag="PLO58_03785"
     CDS             complement(6821..8167)
                     /locus_tag="PLO58_03785"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQO73945.1"
                     /translation="MQELVKIAENVISEARRGGADEVDVLVNNFTNTRLKIRLGKVEE
                     LRQSNPKALGIRVFKNKRKALTYTSDLRPESISKMVQKALEIVRVSSTDEYNGLPEKH
                     LFGKVQADLKLYDPEIAQIPTERKIKMATELEDLGLKQDPLITNSGGSFWNDSYGQSI
                     LINSQGFSGVQEFSSCSLSLQLVAEKDGVKQTDYWWSGQRAFNRLDSIESIAKTAAAR
                     TIRKIGARKPTTQSVPVVYDPQTGADLLAILASVVLGSAIYRKNSFLVDKLYQPIAIE
                     ELNIIDDATLPGGIDSRYFDDEGLPARRNIVIENGILKTYLCDCYSARKLKCTPTGSA
                     RRGTSTDPSPSTSNFFLPAGKYSPEEIIGSVSNGLYLTDLNWVGINYVTGDYSRGAEG
                     IWIENGKLAYPVQEFTVAGNVLEMLKSISMIGNDLEFRGSINSPTFKIDRMMVSGQ"
     gene            complement(8182..9702)
                     /locus_tag="PLO58_03790"
     CDS             complement(8182..9702)
                     /locus_tag="PLO58_03790"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HQO73946.1"
                     /translation="MKRREFIKKSGVIAAGIVTAPAWFDMAGFSKTVSETQDLENLFN
                     ISAVEAEKLLRLALSRGAQSADLFFEYRPNSSLRIEDGIIKDIQYGISLGMGVRAVKG
                     DQIGYAYTDAIDFQSMKRAAETAANIANSTGSFKPARFQKVAAKNLYPVKTYSIDMDL
                     NPKITMLNEADKIARQFNARVSSVIASFSEELRQIMYFDSDGKKFQDIQPLMSLRVQV
                     IAEDGVRRQGASASRSARAGLEFFEKPENTPSAVARQAAATAIINLDAKPAPAGPQVV
                     ILGAGESGVLIHEAVGHGLEADYSYKNLSNYSGKVGQKVAAENCTIIDSGLFPGKRGA
                     LNVDDEGNLPTTTVLIENGILKGYMHNSISARMMKSDPTGNGRRQSYAYPPLARMTTT
                     YLQKGNYEPEEIISSVKKGVYAKGFSGGQVDTIKGDFTFGVTEAYLIEDGKLTAPLKD
                     VTLIGNGPDTMNKILMVGNDLKISDGTWSCGKDGQNIPVSVGNPTVKISEITVGGS"
     gene            10023..10454
                     /locus_tag="PLO58_03795"
     CDS             10023..10454
                     /locus_tag="PLO58_03795"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HQO73947.1"
                     /translation="MVKKSVLFICTGNSVRSQMAEALLRQLGGDQFEVFSAGSTPVGI
                     HRFTKQVLAEIGISVDNQYSKHIDMYRDYNFDYVIVLCEVAALNCPTFPGQVERLEWL
                     IDDPIRVIGNEERRLMAFRITRNILKERIEKFIKEKGNPST"
     gene            10610..13726
                     /locus_tag="PLO58_03800"
     CDS             10610..13726
                     /locus_tag="PLO58_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73948.1"
                     /translation="MIKKLKSILLILIFATAGFSQYGKNKVQYDTFNWSYLQSENMDI
                     YFYEGGESLAEFAAPVGEQALKDICKVLNWRLRKRVTLIVYNSHSDFQQTNVTLSYLY
                     EGIGGFTELFKNRAVVPFEGSYFDFWHVIRHELVHVVINDMIYGGNAQSVVSGRVRLS
                     IPDWMSEGLAEYIAYGWTAQTDLIMRDIALNSNIPNIEELDYYSAYQGGNSVYRFIAE
                     KYGIEKIGQIWAQMKSKGNANNGLKAAIGMDMKELTDKWHRWMRTEYWPDVADRYDIE
                     EISMKLTDHKKLKNYFNTAPAISPLGDKIAIMTDRNGYADIYLISAVDGKVIKKLISG
                     QRTPDLEELKWLNPRLSWSPDGKKVVLAAKSGGHDALIIADVETQKRQRFEFKELEEI
                     FTPVWSPKGNSIAFVGLKNGQTDLYLYDIKKKSLTQLINDKYPDFEPSWSPDGQRIVF
                     VSQRTPEIDSLPNLPEIMNVKCFQTDLFCLDLDNGEITRLTDSPWNENYPCWAHTKDA
                     IFYTSDYHGISNIFMLDISTGESGAITNVLTGVFQPSISNDDLRLVLAGYADEGWDIY
                     SISNPLQLWNERQEIKPTVYAKALMESWENPEKYQRFNFPSKEEAKPRTRQNIIAAGD
                     FSNYIFAPMYNYEISAEPEEDFPDSTMASEDTTKQTTKSGRYPVFPYKTNFSLDVAYG
                     EAGYSNLWGLQGTTVFYFSDVLGNHQLALGTEMYIDLENSDYYLSYMYLAKRTNWGIT
                     GFHTSNFWNYGFFTVFRLRNYGLDFSASRPSSHFSRTELGLTSYNVEQKYILTLTGEE
                     LYTYRINTILPRVGFVYDNSLWGYLYPIDGWRARVDFIASPKYSAKSLEFYSVQADIR
                     RYLKLNMDYSFGLRLSTGFSNGKNAQRFFLGGEDNWINYYYRNDIFTTDSTLAENIYF
                     SDFITPLRGALYYERAGNRYFLFNLEFRYPFIRQLVLGWPLPVTMGGIQGVTFLDFGS
                     TWYDNKFHPFKNDYYRGFMMDDLVAGYGMGARIYLGVFVLKIDVAWRFDFDRTYSPRW
                     YFCLDTDF"
     gene            13814..14722
                     /locus_tag="PLO58_03805"
     CDS             13814..14722
                     /locus_tag="PLO58_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQO73949.1"
                     /translation="MKYFKLILICFIWSTTWAVIKIGLDQTPLMVGLALRFSVATIAL
                     GIIIFLKRRKIAFDRVSVQNYLVVGLLTMALSFYCTYWAEKFIPSGLTSILWTAMPIV
                     VGIFAHFMLPDERLRLAQVMAILIAMAGVIVILSDRQLVFGRELLWGSVVALFGVVVS
                     SWPNVLLKTRPQPYDSLTMTAMAMLIAALVNFIGATVGGEWQKMVWDFKNIGAIVYLG
                     LFGSALAFYIYFDLLKQINVIKLTFINFISPIFATLIGALWLNEIVTWREIGGMLIIF
                     FGLFLYDKDKYLAILKIKNGRSTHAR"
     gene            14712..16064
                     /gene="radA"
                     /locus_tag="PLO58_03810"
     CDS             14712..16064
                     /gene="radA"
                     /locus_tag="PLO58_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQO73950.1"
                     /translation="MPVKVKNVFVCNECGSVQPKWLGKCPECGAWNSMVAEQIQKKPR
                     SSAVERTRRNLVPLNQASTTSTQRLVTGITEFDRVLGGGFVSGSVVLIGGEPGIGKST
                     LIMQVIARLPGDRLLYFSGEESEEQLALRARRLEINDDRILVACENNLENILDQIENL
                     KPTAVVIDSIQTVYSDQFENVPGSVTQVRECAGRLLRLSKELNITTVFVGHITKDGMI
                     AGPKVLEHLVDTVLYLEGNKNNFYRTLRAVKNRFGSTNEVGIFEMKERGLIAVDDPAG
                     FFLTERREYVAGSAVTVSMEGTRPFLIEAQALVTQSSYGTPQRSANGIDYRRFVMLVA
                     VLEKRAGLPLRTQDVFINLAGGFKIDETGIDLGVTVAVASSFKDVPLEPQAAFIGEVG
                     LVGEVRSVPFLENRVREALKFGFTTVYVPKSGIKILEKSLQSKVTGIESLNQLFGFIF
                     "
     gene            16080..17327
                     /gene="tgt"
                     /locus_tag="PLO58_03815"
     CDS             16080..17327
                     /gene="tgt"
                     /locus_tag="PLO58_03815"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQO73951.1"
                     /translation="MSNHGQKESKEKNQLPFDIIAQDTGTKARAGIFVTDHGTVETPV
                     FMPVGTSGVVKTLTPAELQECRVQIILGNTYHLYLRPGLDVIRAAGGLHRFNAWPGPI
                     LTDSGGYQVFSLAHLGKLTEDEIVFRSHIDGSKHRLSPAISMEIQRTLGADIVMAFDE
                     CTPYPCDREYARQALERTHRWERLSREHFLKLKPLYGHRQYQFGIVQGSVYPDLRAES
                     VKALTEVDFDGYAVGGLAVGEPKEQMFEILSLVAPQLPEKKPRYLMGVGKPEDIVRAI
                     GWGIDMFDCVLPSRNARNGTLYTWKGRIILKQSQYRMDFAPPDPDCNCYTCRNFSRAF
                     LRHLFMNGEMTGLRLNTLHNVHFFMELVSRARQAILSGDYENWQTQFFRNYPEEADHS
                     LENEARRSERRRKHLEENDINSV"
     gene            17380..18465
                     /gene="nadA"
                     /locus_tag="PLO58_03820"
     CDS             17380..18465
                     /gene="nadA"
                     /locus_tag="PLO58_03820"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQO73952.1"
                     /translation="MNLNVSYPPPDLARLSQEEIERRIGKIRAQFGPQLIILAHHYQC
                     DQIVAQADAVGDSFALSRRASETKAKFIVFCGVHFMAETADLLKSADQKVFLPNVDAG
                     CPLAEMANIENVKTAWGKIQALTGDQLIPVTYINSRLELKAFCGKNGGIVCTSSNAQA
                     ALEWAWSKKPKVFFFPDQHLGRNVALKMGISPQEIVIYDPRRPIDDKFAWQIKQARLI
                     IWSGFCEVHQAFSRNDIARLRQAIPGIKIIVHPECPEEVALNADFVGSTEYIIKTVTA
                     GAPGSAWGVGTERNLVDRLARQLPDRQVILLQEKGAYCSTMALLTPIHLLYQLECLRD
                     GRLINQVVADENLRSDALLAVQRMMEL"
     gene            18468..20036
                     /gene="nadB"
                     /locus_tag="PLO58_03825"
     CDS             18468..20036
                     /gene="nadB"
                     /locus_tag="PLO58_03825"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQO73953.1"
                     /translation="MKAAEPYRMTDVLVIGGGLAGCSAAYAAAQAGLRVVLLTINQDA
                     SVSSSYYAQGGIIYKNPADTLADYIADFQESGAQLVNEKVVEAIYTNGPRLIEQILIG
                     ELGVPFDRTPQGDIALTREGVHSRPRIAFVKDYTGRAIQERFHKKIRQVENIEVLPNT
                     TCVDLITLAHHSLNPLHIYEPSTCAGAYVYFQDSGEIHPILAKQTILASGGLGRLYLH
                     TSNPYHARGDGYAMAYRAGARLMNMEYLQFHPTTLFSTEDERFLISEAVRGEGGILRN
                     KDGEDFMPRYHPLGSLAPRDVVARSVMQEISRTKAHFVYLDITHKSADWLKQRFPQIY
                     EKCLSVKIDMATEWIPVVPAAHFECGGVAVDLLGNTTIRRLKAVGEVACTGLHGANRL
                     ASSSLLECLVFGTLAGRDCAERIGSVEIITPQVAPWQKENEPLDPDLIHQDRLTIRHT
                     MWNYVGIVRNAKKLDRAGGILWRLQQDIEKFYAHSQLNDELIGLRNAATTTLLVLHAA
                     RLNKFSRGCHYRVD"
     gene            20065..20394
                     /gene="yajC"
                     /locus_tag="PLO58_03830"
     CDS             20065..20394
                     /gene="yajC"
                     /locus_tag="PLO58_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQO73954.1"
                     /translation="MNSLLYAGATGGGSGTQTANPILSLLPFLLIILLMYFLMIRPQA
                     KKQREKAKMLQEIQPGDEIVTIGGIYGKVEGVKDKNILIVRVAKDVKINISRSAVASK
                     VEEKVVS"
     gene            20398..20934
                     /gene="def"
                     /locus_tag="PLO58_03835"
     CDS             20398..20934
                     /gene="def"
                     /locus_tag="PLO58_03835"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQO73955.1"
                     /translation="MAVLPIYIYGSPILHKKLAPIDDLPPDFDRILSDMWETMYDDEG
                     IGLSANQVGLDLRFFVADFSLREENLGREVIINPVIIKTEGEVIGEEGCLSLPNIREQ
                     IPRAEKIILRYENRERKIIEREFNDLVARVIQHETDHLDGLTMVDRISPLKRSFLRSK
                     LKRLATAAAEGKFAKDIE"
     gene            20941..21870
                     /gene="fmt"
                     /locus_tag="PLO58_03840"
     CDS             20941..21870
                     /gene="fmt"
                     /locus_tag="PLO58_03840"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQO73956.1"
                     /translation="MKIVFMGTPEFAVPSLRIIADSRHEIKAVVTKPDMPCGRGCRLQ
                     EPPVKKVARQLNLPILQPDSLKSPDFLAEMAQFSAELYVVVAFRILPPELLVIPPRGA
                     INLHASLLPRYRGAAPINWALINGEKETGVTVFQIKPTVDTGNILLQERVAIQPTDTY
                     GTLAPRLAEIGARLILAAIDGLEAGTLKGTPQDDSLATPAPKIFPELGEIDWYKPASD
                     LKNLIHGLSPTPGAFTFFRGKRLKILRASVIDEKIPGEPGTIIQCNKNSLNVLTGSGV
                     LKLEELQFEGRKPLPAAEFLCGFGGRVGDKFAS"
     gene            21867..23186
                     /gene="rsmB"
                     /locus_tag="PLO58_03845"
     CDS             21867..23186
                     /gene="rsmB"
                     /locus_tag="PLO58_03845"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HQO73957.1"
                     /translation="MNTRAAAVTILNTLPGTGYNYESAIDAFLKINNCSSPDSDFIYL
                     LVKGTIQNQSLLDYLIEQTRNNSVKRLEKIARNLLRVGVLQIVVLKTPPHAAVNETVA
                     AAHQLQRSDLAALINGVLRHIPQESVWRERLGQLELAEALAIEFSHPRWLVEKWLNAF
                     GRENTIDLLAFNNKYQAIFFRHNPLRISWSKFEAKLQADNYRIEIYETAPVVFFLVDR
                     PGDLLKSEYFERGYCSVQDYSQSLAVRLLDPQPGEKILDACAAPGGKATMIAQLTKNQ
                     ANILANDRTINKIESIKESACRLGIDSLNYSVNDAAAAKFPMFDKILLDAPCSGSGVL
                     ARRADLRWSRKPGDLIQAQALQLRLLENLAGFLRDKGVLVYSTCSIEPEENWETVQMF
                     LERHPHFSVDTAVKYVDQRYCDQKGAVMVLPFEHKLTGSFAVRLVKN"
     gene            23208..23942
                     /locus_tag="PLO58_03850"
     CDS             23208..23942
                     /locus_tag="PLO58_03850"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HQO73958.1"
                     /translation="MSTKIRNCLRFIAAFIAVGVVLVLVFDKILMPIYVRHGKEVAMI
                     DVLKLPADKAIQILDANGFEVQVVDTIESGNLPIGIVIDQQPPPGRMVKKGRVVQLVV
                     TGGSRFVRMPNLVGRALKAAQIILENNKLVLDSVEYLYSSEIPEGVVSEQSILPGSMV
                     SINTKVTVVVSKGPPSRQLEVPMLIGLSLTEARASIRKAGFRVGVIRYVPTTDLNPYT
                     VIDQTPKPGGLYDNPINIDLQVTIEP"
     gene            23932..24597
                     /gene="rpe"
                     /locus_tag="PLO58_03855"
     CDS             23932..24597
                     /gene="rpe"
                     /locus_tag="PLO58_03855"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQO73959.1"
                     /translation="MNHNKPTPEVVPSLLSADFARLKEHIRLVEKAGARRLHLDIMDG
                     HFVPNITIGPLVVEAIRRVTNLHLETHLMIERPERFIQQFVEAGSNTVILHVESAQDL
                     HNDLRKIKELGVKAGVVLNPPTSFEEVEPYLDEIDHLLVMTVHPGFGGQQMIPEALDK
                     VRRAQPWQARYGFLIEVDGGINQETITIAAAAGADLLVAGNAVFGSGRPGHAFRSLTW
                     QIK"
     gene            24594..>25194
                     /locus_tag="PLO58_03860"
     CDS             24594..>25194
                     /locus_tag="PLO58_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73960.1"
                     /translation="MIKPVNEGELGRKAIHYFSSLIPIIYHFFFTRNEAVVIIGILFI
                     GIVVAELMRMHLPALRRLYYRLFGWMIRPHEFHNHFTGATYVFMGAFLAIFLFPKEVA
                     ITSLLILTVGDPTACLVGLSIGRIKIYNNKTLEGALAFIFVSLIATVWISSIPFWIKL
                     VGAVVACCVEVFHRKIDDNVLIPLISGTTIFFLVELGNFL"
BASE COUNT         6627 a         5149 c         6179 g         7239 t
ORIGIN      
        1 gttagccgct aaactatcgc ctatttagtg tcgagaaaat atttattttt ttaatcaaaa
       61 gaattccccg atgcttgcgt cggggtacga aaaggggtaa ctttcatgca tgagcgaaca
      121 tataataaaa agtcacaaca aaacattatt actttaccac cttgtatttc ctgtaaaata
      181 tcgctgtggt gtgataaagg aagaattagg tgaaacaata aaggcaatag gtttggagat
      241 atcagagagg tacgagattc actttgttga aataggagca gatgaagatc atgtgcatta
      301 tatggtgcag agtgttccga aattatcagt gacagagata gtgacgacaa tcaagagtat
      361 aacggctaga gagatatttg agcgtcaacc ggagataaga cagcaattgt ggggtggtca
      421 tttttggaca agtgggtatt atgcaaatac ggtagggaga tatgggaatg aattcttgat
      481 acagcagtat gtcaaggaac aagggaaaaa gtatgatcgg atatatcgag gacaattgac
      541 aatgttcgaa gggctgtcct gatcgttgat accccgatgc ttgcgtcggg gtagttcatt
      601 atgctttatt acaatgattt accgaagata gcgcgacaat gttttgtttt atttcaaact
      661 ttttcttgct aattagtcgc aagcgattac cttattatgt gagttgaaat tgtgatgttg
      721 ataacttgtt aatattgggt ggtgctctta ttgtttttaa attatctgtt attgaaggtt
      781 taaatttgag tggatctcct aattttgcgg attttagggt gattattctc gtgggctcga
      841 tagcagactc gtcggaatat ctcccaatat aatttacaat atgagtgggg gctggacgct
      901 aaacggttta tataaatggt gtttaaatgc cttgtattta tgttgataac ttctatttag
      961 gattcgtttt ttatgcctaa ttcattgatt tgcagtgatg tttggaatag ttgcttggaa
     1021 tatgtgcgta aaaagattcc tcagcagacc tttgaaactt ggtttgctcc gatgaaagcc
     1081 atatcgcttg ccgatgatac tctaatttta caggtcccta ataaattttt ttatgattgg
     1141 gttgacaatc actatcggga aatgattctt aaggcgctta aggaggtaac tggtaacggt
     1201 ttcaatgtaa gttatactat tttattgggc gaggaagtgg aacagccagc cggaacccac
     1261 tctgtttcta agcgagggag cggggttact tcggtagctg agccggattt tatcgctggg
     1321 gcacgggagc aaggcacccg cttaaatgag cgttatgttt tcgatacgtt cgttgagggc
     1381 gcttctaacc aatttgcgcg agccgcggcg atggctgtcg ccagtgcgcc ggggcgaacc
     1441 tctttcaatc cgctggtaat ttatggcggc actggtctgg ggaaaaccca tttacttcaa
     1501 gccattggta atgaagctct taataatggc agagccaaga aagtagtcta tgtttccagc
     1561 gataccttca ctttggattt cattgcggca attcagaaca acaagacgac tgaattttct
     1621 cgttcttatc gcagtattga tatgcttctc ctcgacgaca tccaattttt tcaaaataaa
     1681 gaacaaacgc aggagcagtt tttccacatt tttaatgact tatatcaaag gcataagcag
     1741 attgtattaa caactgacaa gccgccaatg gagctgcgcg gtttgcaaga acgtctctta
     1801 tcacgctttc aatccagtct aaccgttgat attcagccgc cagacttaga aaccaggatt
     1861 gctattttgc agaagaaagc taatgatgac ggattggaca ttccctatga agtaattgaa
     1921 tatttatcag ccaatattcg ttcgaatatt agggaactgg aaggagcttt gattcggctt
     1981 ttagcttatt cgtcgttatt gcaagtggat atagatatta atcttactca acgagtaatg
     2041 cgggagattc tgggaactcg tcactcatcg gtgttatcaa tagagagtgt tcaaagtacg
     2101 gtgagtactg tttataagat aaatctggat tctatggttg ggaaggggcg taccaaagaa
     2161 gtggctgagg cacggatgat tgcaatgtat ttaacgcgag agttcaccga tctatcccta
     2221 aaaaccatcg gtttatattt tggcggtcgt gatcattcga cggtagtaca cgcttgccgc
     2281 tgggtggaag agcgcttaca aagcgactcg gtttttgctc gtcagattaa tgatctgaaa
     2341 gccaaattgt cccaaaacat ttagtaaatt ttataaccac agatggcgat ccagactgcg
     2401 gtattgaata gcttcgctga tgtgttcggg taaaattttt tcggaattgc ttaggtcggc
     2461 gattgttcgg gatactttaa gaatccggtc gtaagctctg gcactcaatc ccagcttatt
     2521 aatggcgttg cgcaagagat cttcacaggt ttgatcaatt tgacagaatc ttcggatcaa
     2581 gcgtgattcc atatgcgcat tagcaaagac acccttgatc ccggcaaaac gtgcggtttg
     2641 ttttaggcgg gcggcttgca cacgttcccg gataatttcg gaaggttcgc cgggcgcttt
     2701 cgctgacaga tcggaaaatt taacggcggg aacttctata tgaatgtcaa ttcggtctag
     2761 taatggaccg gaaatacgcg ccatatattt ctggatttgt tggggagtac aggtgcattg
     2821 atgatgagga tcggtggcat agccgcaagg gcagggattc atcgcggcaa ctagcataaa
     2881 ggaagccggg taggttaaag agagtagcgc ccgcgagatg gttacgtagc cattttcaag
     2941 cggctgacgc attacttcga ggacgttttt tttaaattcg ggtaattcgt cgaggaaaag
     3001 tacgccgtga tgactcaaac tgacttcacc agggcggggg aatttaccac cgccgaccaa
     3061 tgcggcatcg gaaatcgtgt ggtgaggcga ccggaacggc cgagtggcta cgatgccctg
     3121 gccggaggga accaaaccgg ctacggaatg tattttggta gtttcgagcg cttcttcaag
     3181 agttaaattg ggcaaaatag tcggaatacg tttggcgagc atcgtttttc ccgaaccagg
     3241 cggacctatc ataattatat tgtgtccccc agccgcggcg acttcgaggg cgcgcttgac
     3301 attttcctga cccttcacct cagaaaagtc aatctggtat tggcgatgtt cttcgaagag
     3361 cgcccggaca tcaacgtgag ttggttccgg atgagtccgg ttttccagga agtctatcac
     3421 ttcctgaagg gattttaagc cgataatttc cagatcgcca gttatagccg cctcgcgggc
     3481 gttctgttcc ggcaagacga tgcccttgat tccggaatta cgtactgaaa ccgagattgg
     3541 caaagcgccc ttaatttcgc gaagcattcc atcaagggag agttcgccca ggaaaatgta
     3601 atcgctaacc ttatgtttct ctatcaaatc agccgcggtg agaataccga tggcaatcgg
     3661 tagatcaaag gagctccctt ctttctttat atcagctggg gctaaattga ccacaatttt
     3721 attgctcggg acatagtagc cgagattttt tatcgcggac caaactcttt ctttactttc
     3781 acggacggcg ccctctggta atccaaccgt aataaaagcc ggtaattgcg cattactcag
     3841 attacattca acatccacaa tataggcatc aatgcctaaa acagctgcac tcttgacgag
     3901 ggaaaatttg gtcataagtt actccttcca aactaaaaaa ttggtaaccg gtcttgataa
     3961 aacggctaac caagcgctat cttattggtc gaaatttaat ttccagtcgc agttcattac
     4021 atttttacgt tgaaattgtt tttcatattg agcaatttcg cgagccattt tttcacaaac
     4081 attatcgtga taagttttag cggcaactat aatggcattg gtaatggctt tggcgcgact
     4141 acggccgtga gccttgatta ctattttgcg aaatccgaga agaggtgcgc caccgtattc
     4201 tgaataatcg gcaacctttt tcaattgttt aatgccactg gaaagcgcaa gcaaaccgag
     4261 catccagata aaacggtgtt taaaagcata gcgtccgatg tgagaaatag tttcaccaaa
     4321 accttcgata gtcttcataa cgatattgcc cacataaccc tcggcgacga tgatatcagc
     4381 tatacctttt aaaatttcgt taccttcgat attacctatg aatttcatat cttggattga
     4441 attaagcata ttaaaagcgt cggtgagagt atcgccacct ttgtaaggtt ctttgccgat
     4501 attaagtagt ccaacggtcg gattttctat acctttgacg gcggaagcat atatttttcc
     4561 cattatagcg aactgtacca gatcatcggc tgtgcaatgg acgttagcgc cgacgtcgag
     4621 taagagggca aatgggtcag accttccctg ttcgttgcga gtaggatagg ctgaggcaat
     4681 ggcagttttg tgaaccccct cgatgcgtgg aatgtacttg atcgctgaaa gaatataagc
     4741 accggtattg ccggccgaga cgataccatg tgcttgatcc gcaccacaga gttgcgaggc
     4801 gattaacatt gaagcgcgcg gttttttgga taggataaat ttcggcttgt cgtcgttggt
     4861 aatatattgg tcggtatgaa caatttcgag ctgttgagga ttgtgttcag tttgttcaag
     4921 aattggttct attttagatt ggtcaccaac taaaatgatt tttatgggag tctcaaccga
     4981 agcgcgagcg gcaccgagca ctacttcatc cggcgcaaaa tcccctccca ttgcatcaac
     5041 tgcaataacg gcagaaggtt gtttattatc taagttattc attttatgtt ttttaggtgc
     5101 ttaatcattt ccgggataat tgcgaaaaga ttagcgacaa ttccataatt agcaatttta
     5161 aatatcgggg cgtcgggatc attgttgatc gcgacgatgt atttggcgga agatattccg
     5221 gcaatatgtt gcattgcgcc ggaaatccca caagcaatat atagatctgg tgagacggtt
     5281 ttaccggttt gcccgacctg aaaagtttgc gggacccaac cggcatcaac aacggaacgg
     5341 ctggcgccga cggccgcacc gagagcgtcg gctaattctt ctatcaagtg aaaattttcc
     5401 ggagcaccga tgccacgacc accagcgaca ataattttgg catcagtgag tgctggtcgc
     5461 tttgaagcaa ctggacgaat ttcattaatt ttaattttgg gagtaacggc agatagctgt
     5521 gacggagagt tgatgatttc tgctgagcga atattttcct gcggcgggat gatattggct
     5581 cggatggtga taaacaccgg tttttttatc aattttaaat cagcgagagc cttaccgcca
     5641 tacatagctc ggcggacgat tggattttgt tctgagtcga gattgacctg taaacaatca
     5701 acggctatag aaccgctaac cgtttcacaa gcaattgccg cgatgtcacg gatagctggt
     5761 cggtcaggta gaatgagaat gtccccattt tcttccagta aaatattggc tacgtctgaa
     5821 gccgttactt ccggtatgta tttagcgaag tttttagtga aggcaactcg gtcagctccg
     5881 tagtgaccac attcattttg catagtacta ctttcctgag ctgggacaat agcagtgagg
     5941 cttccattgg ctaaatcagc caattggcgc ccaagtgtca aacagcccgc gctgttcttg
     6001 gagatatttc cgttttctgg ttcaatatag gtaaatattt tcatcaggat tccccgttat
     6061 aaaactttgg cttcttcgcg cagacgccgg atcaattctg ggactgcatc cacgttctgg
     6121 cctagaatcg taccagctgg ccgtggtgcg ggttctgaca gttgaataat atcggaagcc
     6181 ttttcggtta agttaacatt tttggtaata atctcttttt ttcgagagag cataatatcc
     6241 tttagcgagg gataacgcgg tttattcaag cccttttcgg tagtcaataa acaaggcagg
     6301 gaactctcgt aaattattcg gccttcttcg gtgtctttag cggctgttaa tcggtctgta
     6361 ccaacctgca gttcgcatat tcctgtcagg aaaggatatt cgagtagaca ggctaccaaa
     6421 ggtccgacga gaccattgcc ggtgtcaatg gaaagctttc cgcagaatat caagtccgga
     6481 gagagtggct tgatttctgt ggctaatgcc ttagcagttg ctaaggggtc actgagtgct
     6541 ttatcggcgg tgaccagatg aatcatacgg tcggcgccca gagccagtac gcgctttaag
     6601 ctgatattat cccgctcggg tccgagtgaa atggcgatta cctcgccgcc gtttttttct
     6661 ttcaatagaa cggcggcttc aatagcaaat tcatcgtaag gattcaggtc gaatttaatg
     6721 ttgaccaggt caattgctgt tttttccgga acaatggtta tgatcgaatc ggataccgga
     6781 acaactttga cgcaaactac gattttcatt actcttactc ctactgaccg ctgaccatca
     6841 tccgatcaat tttgaatgtc ggtgaattaa ttgagccgcg gaattccaga tcgttaccta
     6901 tcatactaat tgattttaac atttctaata cattgccagc tacggtgaat tcttgaaccg
     6961 gataggcaag tttgccattc tcgatccaga taccttctgc gccgcgcgaa taatctccgg
     7021 taacgtaatt aataccaacc caatttaaat cggtcagata caagccgttg ctgacagaac
     7081 cgattatttc ttctggtgaa tattttccag ctggcagaaa gaaattcgag gttgacggtg
     7141 aggggtcagt gcttgtgcca cggcgggcgc ttcccgtagg cgtgcatttt aatttacgtg
     7201 ctgagtagca gtcgcataga taagttttta gaataccatt ttcgatgaca atgttacggc
     7261 gggctggcag accttcatca tcgaaatagc gagaatcgat accgcccggc aaagtagcgt
     7321 catcaataat atttaattcc tcaatggcga taggctgata gagtttatct accaggaagg
     7381 aatttttgcg ataaatggca ctgcctaata caacactagc aagtatagca agtagatcgg
     7441 caccggtttg aggatcataa acaaccggga ctgattgggt ggttggtttg cgagccccga
     7501 ttttgcggat ggtgcgcgcg gccgccgttt tggcgattga ttcaatcgaa tccagccggt
     7561 tgaaagcccg ttgacccgac caccagtaat cggtttgttt gacgccatct ttttcggcga
     7621 cgagttgtaa agaaagtgaa cagctagaaa attcctgcac gccggaaaaa ccttgtgaat
     7681 tgatcagaat ggattgacca taagaatcgt tccagaatga gccgcccgag ttggtgatta
     7741 gtggatcttg tttcaggccg agatcttcca gctcggtagc cattttgatc ttgcgctcgg
     7801 taggaatttg agcgatttcg ggatcgtaaa gcttaagatc agcttgcact ttaccaaaca
     7861 aatgcttctc cggcaatcca ttgtattcgt cagtgctgga aacccggaca atttcgagag
     7921 ctttctgtac cattttggaa attgattcgg ggcgcaagtc actggtgtat gttagagctt
     7981 tacgcttatt cttgaaaaca cggatgccga gtgctttagg attggactgt cgcaattcct
     8041 cgacttttcc gaggcggatt ttgagccggg tattggtgaa attatttacg agcacgtcaa
     8101 cctcatcggc gccaccgcga cgcgcctcgg agataacatt ttcggctatt ttgactaatt
     8161 cttgcatatt tttcctcgta attaagagcc gcctacggtg atttcactga ttttgactgt
     8221 gggattaccg acgctgactg ggatattttg accgtctttg ccgcaagacc aagtgccgtc
     8281 agaaattttc aagtcattgc cgaccatcag aattttattc attgtatctg ggccatttcc
     8341 aatcaaggtg acgtctttca acggtgccgt taatttgccg tcttcaatca gataggcttc
     8401 cgttacccca aaagtaaaat cgcccttgat agtatcaact tgcccgccgc tgaagccctt
     8461 ggcgtaaacg ccctttttaa ccgagctgat gatttcttcg ggttcgtagt tgcctttttg
     8521 caggtaagtt gtggtcatcc gagccagagg cgggtaagcg taagattgtc gccgcccatt
     8581 accggtgggg tcagacttca tcatccgggc tgaaatgcta ttatgcatat aacccttgag
     8641 gataccattt tcaatcagaa cggtggtggt aggtaaattg ccttcgtcat caacgttgag
     8701 ggcgccgcgt ttgccaggaa aaaggcctga atcaatgata gtgcaatttt cggcagctac
     8761 tttctgaccg acttttccgc tgtagttaga taggttttta tagctataat cggcttccag
     8821 tccatggccg acggcttcgt ggatcagcac gccgctttca ccggcgccga gaattacgac
     8881 ttgcggtccg gctggtgctg gcttagcatc taaattgata atggcagtag cggccgcctg
     8941 gcgagcgaca gccgatggag tattttcggg cttttcaaaa aattcgagtc ccgctcgggc
     9001 agagcgagaa gcagatgcac cctggcgccg gacaccatct tcggctataa cctgtaccct
     9061 gagactcatt aatggttgaa tgtcttgaaa ttttttccca tcggagtcaa agtacataat
     9121 ttggcgcagt tcctcagaaa atgaagctat tacgctggag acccgcgcat tgaattgtcg
     9181 ggcgatctta tcagcttcat tgagcattgt aattttggga ttgagatcca tatcaatact
     9241 ataggtctta accggatata aatttttagc cgctactttc tggaaacgtg cgggtttaaa
     9301 actccccgta ctattggcaa tattagcggc ggtttcagcc gctcgtttca tactttgaaa
     9361 gtcaatagcg tcggtgtagg cgtaaccgat ttgatcgcct ttaacagcgc ggacacccat
     9421 tcccagagaa atgccatatt gaatatcttt aatgattcca tcctcaatac gcagactgga
     9481 attgggacgg tattcaaaaa atagatcggc gctttgcgct ccacgggaaa gtgccagccg
     9541 gagaagtttc tcagcttcaa ccgccgatat gttaaaaagg ttttccagat cttgcgtctc
     9601 ggatacggtt ttagaaaaac ctgccatatc aaaccacgct ggagccgtaa cgattccagc
     9661 cgctatcaca ccactttttt tgataaattc gcgtctcttc aagccacctc ctcagatggg
     9721 taatattttt ttgtttatag ttattacttt ggaacaattt aatgtcaact tattaattca
     9781 cattaccaga ttcaaatata atatttgagg ttaatatacg aaaaaaagaa tggttttaaa
     9841 attctggaat aaaaataacg cttgttttgt ccaacgaagg aaaaataatt tgttatttgt
     9901 tcaagttttt aaaaatttac cgattaacac acatacagag tattattgtt cggaaaaaat
     9961 taatatattc ggtcgttaat ttgaatttta agagtaagtc gaatctattg gaagtcagtt
    10021 aaatggttaa gaaatcagtt ctcttcatct gcaccgggaa ttcggtgcgg agtcaaatgg
    10081 cggaagctct tctgcgtcag ttaggcggtg atcagtttga ggttttcagc gccggttcca
    10141 cgccggtcgg aattcaccgt tttaccaaac aggtattagc cgaaatcggt atctcggttg
    10201 acaatcagta ttctaaacac attgatatgt atcgcgatta taacttcgac tatgtaattg
    10261 ttttatgtga ggttgccgct ttgaattgcc cgacttttcc cggccaggtt gagcgcctag
    10321 aatggctgat tgatgatccg atccgagtca ttggaaatga agagcggcgt ttaatggctt
    10381 ttaggattac ccgtaatatt ctcaaggaac ggattgagaa attcattaag gagaaaggta
    10441 atccatctac ttaataattg ttaataaact tgggaaagcc agttgattaa cttttgcccg
    10501 atttaatctt ctaaagtttg ttatgatagc ctaacttgac gagggtcgaa aaaatgatta
    10561 cttttgattc agattggaac aacgctattg ataatttgtt gaaaattcta tgataaagaa
    10621 acttaaatcc attcttttaa tactgatttt cgcgacggcc ggattttcac aatacggtaa
    10681 aaataaagtt cagtacgata cattcaattg gtcatatttg caatccgaga atatggatat
    10741 ttatttttat gaaggcggag aatctttggc cgagttcgcg gctccggttg gggaacaagc
    10801 tctgaaggac atttgcaaag tgctgaactg gcgcttgcgc aagcgagtga ctttaatcgt
    10861 atataattcg cacagcgatt ttcaacagac caatgtaacc ctttcatatt tatacgaagg
    10921 gattggcgga tttactgagc ttttcaagaa ccgcgccgta gtgccttttg aaggctcgta
    10981 tttcgatttc tggcacgtga tccggcacga actggtgcac gtggtcatca acgatatgat
    11041 ttatggcgga aatgcccaga gtgtggtctc tggtcgagtg agattgagta ttcctgattg
    11101 gatgagtgaa ggcttggctg aatatattgc ctacggctgg acggctcaaa ccgatttgat
    11161 aatgcgtgat attgctctga atagcaatat tcccaatatc gaggaactcg attattactc
    11221 ggcttatcag ggcggcaact cagtttatcg ttttattgct gaaaaatatg gtatcgagaa
    11281 aatcggtcaa atctgggctc aaatgaaaag caagggtaat gccaataacg gtttgaaagc
    11341 cgccattggt atggatatga aagagctgac tgacaagtgg catcgttgga tgcgcactga
    11401 atattggccg gacgttgctg accggtatga tattgaagag atttctatga aattgaccga
    11461 tcataagaaa ttgaaaaatt atttcaatac cgcaccagcc atttcacccc ttggtgataa
    11521 gatcgccata atgaccgatc ggaacgggta cgccgatatt tatttaattt cggcggttga
    11581 cggcaaggta attaaaaagc tgatcagtgg tcagcgtacg ccggacctcg aagaattaaa
    11641 gtggctcaat ccgcgcttga gctggtcgcc ggatggcaaa aaagtggttc ttgcggctaa
    11701 gagcggaggg catgatgcct taataattgc cgacgtagaa acccaaaaac gtcagcgctt
    11761 tgaatttaaa gaattggaag aaatatttac gcccgtctgg tcgccaaagg gcaattcgat
    11821 agccttcgtc ggattgaaaa atggccaaac cgatttgtat ttatatgata ttaagaaaaa
    11881 atccctcacg cagctgataa atgataaata tcccgacttt gaaccgtcct ggtcgccgga
    11941 cggtcagaga atagtattcg tttctcaacg aaccccggag atagacagcc tgcccaattt
    12001 accagagatt atgaatgtca aatgttttca aaccgatcta ttttgtcttg atttagataa
    12061 cggagaaatt acgcgtctga ccgattcgcc ctggaatgaa aattacccgt gctgggcgca
    12121 taccaaggac gctatttttt atacttccga ttatcacggg atttccaata tttttatgtt
    12181 ggatatatcc accggcgaga gcggtgcgat taccaatgta ttgacgggtg tttttcagcc
    12241 cagtatttcg aatgatgacc tgcgtttagt attggctggc tatgccgatg aaggctggga
    12301 tatttattca ataagtaatc cattacaact ttggaacgag cgtcaggaaa ttaaaccgac
    12361 agtttacgct aaagctttaa tggaatcctg ggaaaatccg gagaaatacc aaagattcaa
    12421 tttccccagt aaagaagaag cgaaaccccg aacacgacag aatataattg ccgccgggga
    12481 tttctctaat tatatatttg caccgatgta taattatgag atcagcgcag agcctgaaga
    12541 agattttccg gattcaacta tggcgagcga ggatactacc aaacaaacca ccaaaagtgg
    12601 cagatacccg gtctttccgt acaaaaccaa cttttcgctg gatgtggctt atggcgaggc
    12661 tggatatagt aacctctggg gcttgcaagg cactacggtt ttttatttca gtgatgtttt
    12721 aggtaatcat cagctggcat taggaaccga gatgtatata gatctggaaa acagtgatta
    12781 ttatttatca tatatgtatc tcgccaagcg taccaactgg ggcataaccg gatttcatac
    12841 gtcgaacttt tggaattatg gattttttac ggtatttcgt ttaagaaatt atggacttga
    12901 tttttccgca tcgcgtcctt cgagtcactt ttcgcggact gagctagggc tgacttccta
    12961 taatgtcgag cagaaatata tcctgactct aaccggagaa gagttataca catatagaat
    13021 taacacaatt ttgccacggg tcggttttgt ttatgataat tcactatggg gttatctata
    13081 tccgattgac ggttggcgcg cccgggtgga ttttatcgcc agcccaaagt acagcgccaa
    13141 aagtctggag ttttatagtg ttcaagctga tattcgtcgt tatttaaaat taaacatgga
    13201 ttattctttc ggtttgcgcc tctcgaccgg tttcagtaat ggtaaaaatg ctcagcgttt
    13261 ctttctcggt ggcgaggata attggataaa ttattattac cgtaatgaca ttttcaccac
    13321 cgactcgacg cttgctgaga atatctattt ttcagatttt attacgccat tgagaggggc
    13381 tctgtattat gaaagagccg gtaatcgcta ttttctgttt aatctggagt tccgctaccc
    13441 gtttattagg caacttgtgt taggctggcc gctaccggta acgatgggtg ggattcaagg
    13501 cgtaactttt ttagatttcg gttcgacctg gtatgataat aaatttcatc ccttcaagaa
    13561 tgattactat cgtggattta tgatggatga cctggtagct ggatatggta tgggagcgcg
    13621 catttatttg ggagtttttg tattaaagat tgacgtcgcc tggcggtttg atttcgaccg
    13681 gacttatagt ccgaggtggt atttctgtct cgatactgac ttttaaataa atttttaagc
    13741 tagtttttaa aaaggcttgg tcttaatact gacgctaatt agtttagcga gttattaatt
    13801 attcagaacg aatatgaaat atttcaaatt gattcttatt tgttttatct ggagcactac
    13861 ttgggcggta atcaagattg gtctcgatca aacccccctt atggtcggac tggcgttgcg
    13921 tttcagcgtg gcgacaatcg cgctgggtat tattattttt ctgaaacgcc gtaagattgc
    13981 ctttgaccgt gtatcggtac agaattactt ggtagttggt cttttgacta tggcgcttag
    14041 tttttactgc acttattggg cggaaaaatt tatcccgagc gggttgactt caatcttatg
    14101 gacagctatg ccgattgttg tggggatatt tgctcatttt atgttaccag atgaacgttt
    14161 gcgcttagcc caggtgatgg cgattttaat agcgatggct ggtgtgatag ttatcctttc
    14221 cgatcgccag ctggtttttg gtcgagaatt attatggggc agtgtggttg ccttatttgg
    14281 ggtagtcgta tcttcctggc cgaatgtttt actcaagacg cgtcctcaac cttatgatag
    14341 tttgacgatg actgctatgg caatgttgat tgccgcactt gtgaatttta taggagcaac
    14401 ggtcggcggc gaatggcaaa aaatggtctg ggacttcaaa aatatcgggg cgatcgttta
    14461 tcttggtctg ttcggttccg cgctcgcatt ttatatttac tttgatcttt tgaagcaaat
    14521 taatgtgatc aagctgactt ttattaattt catttcgccg atatttgcga ccttgatcgg
    14581 agcattgtgg ttgaatgaaa tcgttacctg gcgtgaaatt ggtgggatgc tcattatttt
    14641 cttcggacta tttttgtacg acaaggataa atacttagcc atattgaaaa taaaaaacgg
    14701 aaggagtacc catgcccgtt aaagtcaaaa atgtctttgt atgcaatgag tgcggttcag
    14761 ttcagcctaa atggctgggc aaatgtccgg aatgtggcgc ctggaattcg atggttgctg
    14821 aacaaattca gaagaaaccg aggagctccg ctgtcgagag aactcggcgt aatttggtgc
    14881 ctcttaatca ggcaagtacc acctcgaccc agcggttggt taccggcata accgagtttg
    14941 atcgcgtgtt gggcggcgga ttcgtcagtg gttcggtagt attaatcggc ggtgagcccg
    15001 gcattggcaa atcaactcta attatgcaag tcatcgcccg actgccgggg gaccgtttac
    15061 tctatttctc tggcgaagag tccgaagagc aattggcttt gcgcgctcgg cgactggaaa
    15121 ttaatgatga ccgtattctg gtagcctgtg agaataatct ggaaaatatt ttagatcaaa
    15181 tagagaacct gaagccgacc gcggtggtaa ttgattcgat tcaaaccgtt tactccgatc
    15241 agtttgaaaa tgtcccgggc agtgtaactc aggtgcgcga atgcgccggg cgacttctgc
    15301 gtctttccaa ggaattaaat ataaccactg tttttgtcgg tcatattacc aaagatggta
    15361 tgattgccgg gccaaaggtt ttggaacatc tggtagatac ggttttatat ctggaaggca
    15421 ataaaaataa tttttaccgt accttgcgag cggtcaaaaa tcgctttggt tcgaccaatg
    15481 aggtcggaat attcgaaatg aaagagcgtg ggctaatagc cgttgacgac ccagccggtt
    15541 tctttttaac ggagcgtcgt gaatacgttg ctggctcggc ggtcacggtc agtatggaag
    15601 gcacgcgccc ttttctgata gaggcccagg cattggtgac gcaatcgtct tacggcacgc
    15661 cgcagcgttc ggcaaacggg attgattacc gacgctttgt gatgttggta gctgttctgg
    15721 agaaacgcgc cggtttgcct ttgcgcactc aggatgtttt cattaatctg gcgggtggtt
    15781 ttaaaattga tgagacggga attgacctgg gtgtgacggt ggcggtagcg tcgagcttta
    15841 aagatgtccc actcgaaccg caagccgctt tcatcggtga agttggatta gttggtgaag
    15901 tccgcagtgt tccattttta gaaaataggg tacgcgaggc gctcaagttt ggttttacca
    15961 cggtctatgt acccaagtcg gggataaaaa ttttagaaaa aagcctgcaa tccaaagtaa
    16021 ccggcatcga atcgctaaat caactattcg gatttatttt ctaagttcgg aagattgata
    16081 tgtcaaatca cggccagaag gaatctaaag agaaaaatca actgccgttt gatattattg
    16141 cccaagatac cggtaccaaa gcccgggcag ggatatttgt tactgatcac gggacggttg
    16201 aaacgccggt ttttatgccg gttggaacaa gcggagtagt caagactctg acgccggctg
    16261 aactccagga gtgccgcgtt caaattatac taggcaatac ttatcacctt tacttacgac
    16321 cgggactcga tgtaattcgg gcggctggcg gtttgcatcg cttcaatgcc tggccgggac
    16381 caatattgac tgacagcggc ggttatcagg tgttcagcct ggctcatctc ggtaaattga
    16441 ctgaggatga gattgtattc cgttcccata ttgacggttc gaagcaccgt ctatctccgg
    16501 caatttccat ggagattcaa cgaacgctcg gcgctgacat tgtgatggca ttcgacgagt
    16561 gcacacccta tccctgcgac cgggaatatg ccagacaagc tctggagcgt acccatcgct
    16621 gggaacgact ctcgcgggag cattttctga aattaaaacc gctctatggt catcgtcaat
    16681 atcagtttgg aatcgttcag ggctcggtat atcccgattt gcgggcggag agcgtaaagg
    16741 ccttgaccga agtggatttc gacggttatg cagttggtgg gttggcggtc ggtgaaccta
    16801 aagaacagat gtttgaaata ttatctttgg ttgcaccgca attgccggaa aaaaagccgc
    16861 gttatttgat gggagtgggt aaaccggaag atattgtacg cgctattggc tggggaattg
    16921 atatgtttga ctgtgttttg ccgtcgcgca atgcccgcaa cgggacactt tatacctgga
    16981 aagggcgcat aatattgaaa cagagtcaat atcgaatgga ctttgcaccg cctgatccgg
    17041 attgcaattg ttatacttgc cgtaattttt cacgagcttt tctccgccat ctttttatga
    17101 atggcgaaat gaccggattg cgcttgaata ctcttcacaa tgtccacttt tttatggagc
    17161 tggtcagccg tgcccgtcag gcgattttaa gcggtgacta tgagaattgg cagacccaat
    17221 tttttcgtaa ttaccccgaa gaggccgacc attcgctcga aaacgaggcg cgccgctccg
    17281 aacgtcgccg taaacatctt gaagaaaatg acataaatag tgtgtaactt atgcgtcaat
    17341 ttttgaaaaa gtcattatta gaaagtattt gcaatataga tgaaccttaa tgtttcctat
    17401 cccccgcccg atctggcacg tctatctcag gaagaaattg agcggcgcat tggcaaaatc
    17461 cgggctcaat ttggtcccca actaatcatt ctggcgcatc attatcaatg tgaccagatt
    17521 gttgcccaag ccgatgccgt cggcgattcg tttgccctgt cgcgtcgagc atctgaaacg
    17581 aaggcaaagt ttatcgtatt ttgcggggta cattttatgg cagagacagc tgatttattg
    17641 aaaagcgccg atcaaaaggt gtttttacca aatgtagatg ccggttgtcc actggcagag
    17701 atggctaata tagaaaatgt taaaaccgcc tggggaaaaa ttcaggcgct taccggggat
    17761 cagttgattc ccgtaaccta cattaactcg cggctggagc tgaaagcctt ttgtggcaaa
    17821 aatggcggta tagtttgcac ctcctccaat gcgcaagccg ctctcgaatg ggcttggtca
    17881 aaaaagccaa aggtgttctt ctttccggat cagcatctcg gtcgcaatgt agctttaaaa
    17941 atgggaatct cgccgcagga aatcgttata tatgatccga gacgaccaat tgatgacaaa
    18001 tttgcatggc aaattaaaca agcccgcctg ataatctgga gtgggttttg cgaggtgcat
    18061 caggcttttt caagaaatga catagcaaga ctacgccagg ctattccggg tattaagatt
    18121 atcgttcatc cggagtgtcc ggaagaggta gcgttgaatg ctgattttgt agggagtacc
    18181 gaatatatta ttaagactgt tacggcaggg gcgccgggca gcgcctgggg agttggtacc
    18241 gagcgaaatt tggttgatcg tctggcgcgc cagttgccag accgccaggt gattttattg
    18301 caagaaaaag gtgcatattg ctcgacaatg gctttgttaa cacctatcca cttgctttat
    18361 caattggaat gtctgagaga tggccgcctg atcaatcaag tggtagcaga tgaaaatttg
    18421 agaagcgatg cgcttcttgc cgtacaaaga atgatggagt tgtagctttg aaagccgctg
    18481 aaccttatcg catgactgat gttttggtga tcggtggagg cttagccggt tgttcggcgg
    18541 cttatgccgc ggctcaagcc ggattgcgag ttgtattatt gactatcaat caggatgcca
    18601 gtgtctcgtc gtcttattat gcgcaaggtg gtattatcta taaaaatcca gccgacacgc
    18661 tggcagatta catagcagat ttccaggaat cgggagcgca attagtaaat gaaaaagtag
    18721 tcgaagcaat ttacaccaat ggaccgcggc tgatagaaca gattcttatt ggtgaattgg
    18781 gggtgccgtt tgatcggacg ccgcagggtg atattgccct aactcgcgag ggagttcatt
    18841 ccaggccaag aattgctttt gtcaaggatt ataccggtcg ggcgatccag gaacgttttc
    18901 acaagaaaat cagacaggtc gaaaatattg aagtattgcc taatactact tgtgtggatt
    18961 tgataaccct ggcgcatcat tctttaaatc cgcttcatat ttatgagcca agcacctgcg
    19021 ccggggctta tgtatatttt caagacagtg gtgagattca tccaattctt gccaagcaaa
    19081 ccatattggc ttcgggtgga ttggggcggt tgtatttgca tacatcgaat ccctatcatg
    19141 ctcgcggtga cggttatgct atggcgtatc gggcaggcgc acgtctaatg aatatggaat
    19201 atctccaatt tcatccgacc acgctgtttt caacggaaga tgaacgcttt ttaattagcg
    19261 aggctgtgcg gggcgagggt ggtattttac ggaataaaga tggcgaagat ttcatgccgc
    19321 gctaccatcc gctgggttca ttagcgccgc gtgatgtcgt tgcccgaagc gttatgcagg
    19381 aaatcagtcg caccaaggct cattttgtct atctggacat tactcataag agcgccgatt
    19441 ggctgaagca gcgttttccg cagatttacg aaaaatgtct atcggtgaag attgatatgg
    19501 caaccgaatg gattcccgtg gtgcccgccg ctcactttga atgtggcgga gtggcggtgg
    19561 atcttctggg caacactact attcggcgtt taaaagctgt cggtgaagtg gcttgcactg
    19621 gcttacatgg cgctaatcgt ctggcaagca gctctcttct ggagtgttta gtttttggga
    19681 cactggcggg acgcgattgt gccgaacgaa tcgggagtgt ggagatcatt actcctcaag
    19741 tagccccttg gcaaaaagaa aacgagccgc tcgacccgga tcttattcac caggatcgtc
    19801 tgactatcag acataccatg tggaattatg ttggtattgt ccgaaatgcc aagaagctag
    19861 accgagccgg tggaatttta tggcgcttac aacaagatat cgaaaaattt tacgcccata
    19921 gtcaattgaa tgatgagttg atcggcttgc gcaatgccgc tacgacgaca ttgctggttt
    19981 tacatgccgc tcgcctgaat aaattcagtc gcggttgcca ttatcgtgta gattaattaa
    20041 aattgtaata aaaatggagg ttatatgaat agtctgttat atgctggagc aacaggtggt
    20101 ggtagcggaa cccagacagc caatcccatc ctttcattgt tgccgttttt gctgatcatt
    20161 ttgttaatgt attttttgat gattcgcccg caggctaaaa aacagcggga aaaagccaaa
    20221 atgttgcagg aaattcagcc gggggacgaa attgttacta tcggcggaat ttatggcaaa
    20281 gtcgaaggcg taaaggataa aaatatcctg attgtgagag tagctaaaga tgtcaagatt
    20341 aacatatctc gttcggcagt tgccagtaaa gtagaggaaa aagtagtttc gtaaaagatg
    20401 gctgtacttc cgatttacat ttatggtagt ccgattctac ataaaaaatt ggctcctatt
    20461 gatgatttgc cgccagattt cgaccgcatt ctttccgata tgtgggagac gatgtatgat
    20521 gatgaaggaa ttggactttc ggctaatcag gtgggacttg atctacgatt tttcgtagcg
    20581 gatttttctt tgcgggaaga gaatttaggg cgtgaggtca taattaatcc ggtgataatc
    20641 aagacggaag gcgaggttat tggcgaggaa ggttgtctga gtctgcctaa tattcgagaa
    20701 cagataccgc gggctgagaa aatcatcctc cgctatgaaa accgagagcg caagattatc
    20761 gagcgcgaat ttaatgattt ggtagcgcgc gtcattcaac acgagacaga tcatcttgac
    20821 ggattaacta tggttgaccg catcagtccg cttaagcgca gttttttgag gtcaaagctg
    20881 aagcgccttg cgacggccgc tgctgaaggc aagttcgcca aagatattga gtgagttcag
    20941 atgaaaatcg ttttcatggg cacgccggaa tttgccgtgc cgtccttgag aataatagcc
    21001 gactcgcggc atgagattaa ggcggtagtt accaagccgg atatgccttg tggccgtgga
    21061 tgtcgccttc aggaaccgcc ggtaaaaaaa gttgcacgcc aattgaactt gccgatctta
    21121 cagccggact cgcttaagtc accggatttt ttagctgaaa tggcacaatt ttcggctgaa
    21181 ctttatgtag tggttgcctt tcggattttg ccacccgaac ttctggtaat tccaccacgc
    21241 ggcgcgatta atttacatgc ttccctcttg ccgcgctatc gtggtgcggc accgatcaat
    21301 tgggcgctca tcaacggtga aaaagaaacc ggcgtgacgg tgttccaaat aaagccgacc
    21361 gttgataccg gtaatatctt attgcaggaa agagttgcga ttcagcctac tgatacttat
    21421 ggaacgctgg cgccgcgttt agcggaaata ggtgcgcgcc tgatcctggc ggctatagat
    21481 ggtctggaag ccggaactct aaaaggcaca cctcaggatg atagtttggc tacacctgcc
    21541 cctaaaattt tccctgaact tggagaaatt gattggtaca agccagctag cgatttgaaa
    21601 aacctgattc acggattatc gcctactccc ggagctttta cctttttccg gggcaaaaga
    21661 ttgaaaattt tgcgcgcttc tgtcatagat gaaaaaattc cgggtgaacc gggaacgatt
    21721 attcaatgta ataaaaacag cctgaacgta cttaccggta gtggagtttt aaaactggaa
    21781 gagttgcaat ttgaagggcg caaaccttta cctgccgctg aatttttatg cggttttgga
    21841 ggacgagtgg gagataaatt tgcctcgtga atactcgtgc cgccgctgtt acgattttga
    21901 atactttgcc aggcaccggc tataactacg agtcagccat tgacgccttt ctcaagatta
    21961 ataactgcag ctcaccggac agcgatttca tctatctgtt ggtaaaaggc actatccaaa
    22021 atcaaagctt gctggattat ttaatcgagc aaacacggaa caattcagtc aaacgccttg
    22081 agaaaatagc tcgcaatcta ttgcgagtcg gagttctgca gattgtggtt ttgaaaacgc
    22141 cgccccacgc ggcggtgaat gagaccgtag cggccgccca tcagcttcag cgctcggatt
    22201 tagccgcctt gattaatggt gttttacgac atatacccca ggaatcagtc tggcgtgagc
    22261 gtctggggca attagaattg gcggaagcgc tggcaatcga attttcccat ccgcgctggt
    22321 tagtagagaa atggctgaac gctttcggtc gtgagaatac gatagatttg cttgctttta
    22381 ataataaata tcaagcgatt ttttttcggc acaacccact gcgcattagt tggagcaaat
    22441 ttgaggcaaa actacaggct gataactacc ggattgagat atatgaaacc gctccggtag
    22501 tcttttttct agttgaccga ccgggtgact tacttaaaag tgaatatttc gagcgcggtt
    22561 actgttcggt tcaggattat agtcaatcgc tggcggtgcg tcttctggat ccgcaacctg
    22621 gcgaaaaaat attggatgca tgtgcggcac ccggcggcaa ggcgactatg atagcacagt
    22681 tgacgaaaaa ccaagccaat atcctggcaa atgatcgcac gattaataaa attgagtcaa
    22741 tcaaagaatc cgcctgccga ttagggattg attccttaaa ttacagtgtg aatgatgccg
    22801 ccgcagcgaa atttcctatg tttgataaaa ttctattaga tgcaccgtgt agtggtagcg
    22861 gcgttctggc gcggcgggct gacttgcgct ggagtcgtaa accgggggat ttgattcagg
    22921 cgcaagcctt acaattacgg ctactcgaaa atttagcggg atttctgcgt gataaaggcg
    22981 ttttagttta tagcacttgt tccattgagc cagaagagaa ttgggaaacg gtgcaaatgt
    23041 ttctagagcg ccatccgcat ttttcagtgg atacggctgt aaaatacgtt gatcaacgct
    23101 actgtgatca aaagggggca gttatggtct tgccatttga acataaactg accggaagct
    23161 ttgcggtccg tttggtaaaa aattaaaata gaagtagtca ggagcaaatg tcaacaaaga
    23221 ttcgcaactg tttgagattc atagcggctt ttatagcggt cggcgtagtg ctggtactag
    23281 tttttgataa aatattgatg cctatctatg ttcgtcatgg aaaggaagtc gcaatgattg
    23341 atgtgctcaa gctaccggcc gataaagcta tccagatatt agatgccaat ggttttgaag
    23401 tccaggtcgt agatactatt gagagtggta accttcccat agggattgtt attgatcaac
    23461 agccgcctcc tggacggatg gtcaagaaag ggcgagttgt acaattagta gtcactggtg
    23521 gtagccgttt tgtgcgtatg cccaatttgg tcggccgtgc tctgaaagcc gctcaaatca
    23581 tcttggaaaa taataagctc gttctcgatt cggttgaata tctctattcc tcagagattc
    23641 ctgaaggagt ggtcagcgaa caatctattt tgccgggttc aatggttagc attaacacta
    23701 aagtcacagt tgtggtcagt aaaggcccgc caagccgcca acttgaagtg ccgatgctaa
    23761 ttgggctcag cctgactgaa gcccgagcta gtatccggaa agccgggttc agagttggtg
    23821 ttattcgcta tgtaccgacc actgatctta atccatatac agtgattgat cagactccta
    23881 aaccgggcgg tttatatgac aatcctatta acattgattt gcaggtaacc attgaaccat
    23941 aacaagccga caccggaagt cgttccctct ctcctgtcgg ctgattttgc ccgactgaaa
    24001 gaacatattc gcctggttga gaaagctgga gcgcggcgtc tgcacctgga cataatggat
    24061 gggcactttg ttcccaatat taccattgga cctcttgtag tggaagccat taggcgtgtg
    24121 acgaatctgc atctcgagac ccatttgatg atcgagagac cagagcgctt tattcagcaa
    24181 ttcgttgaag ccggtagtaa tacggtaatt ttacacgttg aatcagccca ggacttgcat
    24241 aacgatctcc gtaagatcaa agaattggga gtaaaagctg gtgtagtact gaatccaccg
    24301 acaagctttg aggaagtcga accgtatttg gatgaaattg atcacctgct ggttatgacg
    24361 gttcatccgg gattcggtgg tcagcagatg attcccgagg cactcgacaa agtacggcga
    24421 gcccagcctt ggcaggcgcg ctatggtttt ttgatcgaag tggatggtgg cattaaccag
    24481 gaaacaataa cgatcgccgc ggcggctggt gccgatttat tagttgcagg caacgctgtc
    24541 tttggtagtg gacgcccggg tcatgccttt cgatccttaa cctggcagat aaagtgatta
    24601 aaccggtcaa tgagggggaa ctcggtcgca aggcgattca ttatttttct tcccttatac
    24661 caataatcta ccactttttc ttcacaagaa atgaggcagt agtaattatc gggattctat
    24721 ttataggaat agttgttgcg gaattgatgc gtatgcacct tccggcactg cgtcggctct
    24781 attatcgttt gtttgggtgg atgatcagac cacatgaatt tcataatcat tttactggag
    24841 cgacatatgt atttatgggc gctttcctgg cgatatttct attcccgaaa gaggttgcca
    24901 taacgagtct tttaattctg acagttggcg atccgaccgc ttgtctggtt gggctctcaa
    24961 ttggcaggat aaaaatttat aataacaaga cccttgaagg ggcgttagca tttattttcg
    25021 tatcacttat agcgacggta tggattagtt cgatcccctt ctggataaaa ttggttggcg
    25081 ctgttgtcgc ttgttgtgtg gaagtttttc atcgcaagat tgatgacaat gtactgattc
    25141 ccctcatcag tgggacgacg atttttttcc tggttgaatt gggtaatttt ctaa
//
LOCUS       DAOMJU010000025        24716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1870_length_24716_cov_20.567252, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000025 DAOMJU010000000
VERSION     DAOMJU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 24716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24716
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1870_length_24716_cov_20.567252"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..614)
                     /locus_tag="PLO58_03865"
     CDS             complement(264..614)
                     /locus_tag="PLO58_03865"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HQO73961.1"
                     /translation="MSSLIMLKSKIHNARVTATNLEYEGSIEIDAELIRLANLHVFEK
                     VLVLNLNSGARFETYVIEGKAGQRDISLNGAAARLVEEGDRIIILAFAVVSPAELGDW
                     HPTIIRLDANNNPL"
     gene            826..1368
                     /locus_tag="PLO58_03870"
     CDS             826..1368
                     /locus_tag="PLO58_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQO73962.1"
                     /translation="MFRLINQELSNPVFDWFMPFISSKSFWLIPIFIGLVLLFWLGKK
                     RGRIATLLIILTIATADPFCARVLKPTFKRLRPSHELSDVRLLGKKGGKYGFPSNHAA
                     NVAGSMLILAFFYRRYKYICGVLAFLVGYSRIYLGVHYPTDVLAGFVIGIGISVGWIL
                     IWRSLTNHLLKKRNDLLALS"
     assembly_gap    1427..1526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1541..3340
                     /gene="glmS"
                     /locus_tag="PLO58_03875"
     CDS             1541..3340
                     /gene="glmS"
                     /locus_tag="PLO58_03875"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQO73963.1"
                     /translation="MCGIVAYIGNQSAVPLLLEGLKKLEYRGYDSAGICIAGDHRLEV
                     VKRSGKIAELEKTAALDSFSSHIGIAHTRWATHGAPNEINAHPHLSCHGEIAIVHNGI
                     IENFRSLRDMLIQEGHHFSSETDSEVLAHLIEKFYEGDLATAVVKALHNVEGTFGIAV
                     MHKDEEHLVIARRGSPIIIGLGNQETIVASDAVSIIPHTKKVIYLNDNEIAVIGYNNY
                     RLQNLDGQSIANEIQEIRWNISQIEKRGFKHFMLKEIFEQPEAVQNTLRGRITNGQIK
                     LSLNTDFNRVQRIIIAACGTSWHSALIGKNLIEKYTGIPTEVDYASEFRYRESIINGE
                     VLMIVISQSGETADTLAALRKAKSAGTQTLGIINVVGSTIAREVNSGIYLHAGPEIGV
                     ASTKAFICQVIALLLLALYIKQQKGELIDPALLAKIDMIPEQIRQVAEHSDNLREIAS
                     AYYLNRNFIYLGRGINYPVALEGALKLKEISYIHAEGYPAAEMKHGPIALIDENMPVV
                     FIATKGAVFDKVLSNMQEVKARGGQILTITDHNNPHLQELSNHLIVVPETSEDLSPLI
                     NVIPLQLLAYHIADLKGLNVDKPRNLAKSVTVE"
     gene            3474..4808
                     /locus_tag="PLO58_03880"
     CDS             3474..4808
                     /locus_tag="PLO58_03880"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQO73964.1"
                     /translation="MLKEISLGIILMFSLWVTGQTAEPFFPYKYNVEVLPNGLKVITI
                     PMESNGIVAYYSITRTGSRDEWEPGHSGFAHLFEHLMFRGTEKYPGSLYDKTITELGA
                     DANAYTTDDYTCYHLNFAAEDLEKIIEMESDRFINLAYKEEEFKTETGAVYGEFLKGR
                     VSPWFVLYEKLYDVAFDKHTYKHTTIGFEADVKAMPTMYKYSKTFFQRYYRPENVVLL
                     IVGQIETEKTLELVRKYYGDWQPGYVSPQIEPEPPQTAERKAEVIYPGKTLPIVMVAY
                     KSDAYDPNNRLYVAGLLLEQLAFGENSDLYKRLVLKEQKVEFITADFSVSRDPKLNEI
                     YAMVKSPDDIDYVREQIFMTMEQFKTEAVPESRLEDLKKHLKYSFLMNLDTPDNVAGN
                     LANYVAITGGVETVDQLFNQIMNVTVTDIQQAAQYYGVPEKRTVVILKGVAQ"
     gene            4805..6316
                     /locus_tag="PLO58_03885"
     CDS             4805..6316
                     /locus_tag="PLO58_03885"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQO73965.1"
                     /translation="MKTKLTLLLSWGIMASILLSNCGSKIDASRCVLLPVAEDPTVSY
                     RIYFKVGSQDDPAGKEGLAFLTAHMLSEAATQSNSYEEIIAKLFPLAASYDATVSAEM
                     TVISGRVHRDNLPAYYPLFTEAILKPAFQEDDLNRLKSNVLNYLENVLKYANDEELGK
                     AVLYNEIFAGTGYGHPTEGLINSINSITINDVKDFYQKMYNRNNVIIGVGGGYDNETL
                     AKLQKDLAQLPRGVENIATRAVPYDIQGLKVTIVEKDANATAISLGFPINITRENPEW
                     YALAVANSWFGEHRNSSSHLYQVIRETRGLNYGDYSYIEHFPNSGYYQFPPVNVARHQ
                     QIFEIWIRPVPNETRHFVLRATMRELHNFIENGLTPEQFNLTRNFLRKYILHYAPTTT
                     MRLGYAIDDKFYGIKGSHLENFRIALDNMTVNDVNSAIRKYLQYDNLQIAIVTNEAEK
                     LREDLLANTPSPIKYVTPKPDVVLKEDLEIANYPLAIQPENIKIVNIRDLFYE"
     gene            6309..>6588
                     /locus_tag="PLO58_03890"
     CDS             6309..>6588
                     /locus_tag="PLO58_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73966.1"
                     /translation="MNNSAKNFTDSRTKDVKQCFDISTELRLDNKYYRKFVLTFFDNL
                     LDFDISSKDASLQPAKYYRRQVTAVINAKAPAIIAGMAEIQAFLDHHNL"
     assembly_gap    6589..6688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6689..8923
                     /gene="selD"
                     /locus_tag="PLO58_03895"
     CDS             <6689..8923
                     /gene="selD"
                     /locus_tag="PLO58_03895"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HQO73967.1"
                     /translation="FISISTIINFAPMKPIYLDYNASTPLLPEVVDAMLPFLKNVFGN
                     PSSSHFYGQESKQAIENARRQVAEMLNCQPEEIVFTSGGSESNNFAIRGIAEMHPGGN
                     IITSTIEHPAVLEVCRYLESKNYSVTYLPVNSDGLVELPAVQAAIRPDTFLITIMLAN
                     NEIGTIQPIREIAALAHKHGILVHTDAAQAVGKIVVDVRELDVDLLSVAGHKMYAPKG
                     VGALFIRTGTQIAPLIYGAGHERGLRPGTENVLEIVGLGKAAEIFRAKSAEIVSRQKA
                     LGEKFFNDLRTAIPELGLNGHPTQKLTNTFNVYFPGIDANTMLAELTQVAASAGAACH
                     ASSITPSHVLKALGLPNERILGSVRFSLGRFTTEDEIADAVSAISQTYHRLTSTEEIS
                     IPITSNEPIRLTQFTHGLGCACKIRPQTLEKILREIRPRMALNVLVDFSASDDAAIYK
                     INDEQAIVATVDFFTPIVDDPYDFGRISAANSLSDIYAMGAEPLFALNVVAFPEKRLP
                     VEILTQILRGAQVIADQAGIPILGGHTVEDNEPKFGLVAIGIIHPEKIIRNIGAQPGD
                     ALILTKPIGVGILSTALKRGLLDELTKNLIIANMTELNATAAEVMKSFPVHACTDITG
                     FGLLGHLHEMSTGSRVRAQISASSVPILPSTNEMVLRNVIPGGTEANLSFISPMVIWD
                     KMITDSDKIILCDAQTSGGLLIAVADEFANDMLAQLHQAGVINAAKIGKIESGADGKI
                     KVVP"
     gene            8950..9870
                     /locus_tag="PLO58_03900"
     CDS             8950..9870
                     /locus_tag="PLO58_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73968.1"
                     /translation="MTRFRKEYSKIARKLTSADLQSGIGNSELSLDNIVSDGRLAVDS
                     QFFMGVFSRQGLLNAIRAFGLLDILERKGLTNITIDIDTSDPYIHRLYAYSGKPLNEN
                     KICELVLKQGPLHFENKPLSSFPRRTANILYIEWLLLQNPREKFSPEKPPLPGQNYPG
                     LGIGDRLMEIMILMTRRLNLEGIINKPHYFHTAFMFSKEFVFINPYNQALMYAISKDL
                     LAKYSFWMVAWAAHFNCIINKNDGKPMDWKTGDLILPFTKDLIKYFRGREYQQEVSRL
                     IANFSFEIDLDKLHTALKSNDLEKYARFPK"
     gene            9885..10553
                     /locus_tag="PLO58_03905"
     CDS             9885..10553
                     /locus_tag="PLO58_03905"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HQO73969.1"
                     /translation="MTPNTPINFLSPTALVLGNGNYPNWFDIKQFQVEYKVLLVCADG
                     GAEKARQLNLMPDLIIGDMDSVTTDTINHFQSSEVKIIHQPNQNNNDLEKCLRWLLRH
                     GWRQFVLVGFTGRRDDQTIATLQIMRKYARRAQFLLYTETAQIYLLKKGIWLINAIPG
                     QTISLFGFPRVYHLTTRGLQYPTKDENLTGGSHGLSNTAIDEMVQIQFSSGYLLVVAI
                     QELN"
     gene            10550..11938
                     /locus_tag="PLO58_03910"
     CDS             10550..11938
                     /locus_tag="PLO58_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HQO73970.1"
                     /translation="MTSLRLAPIDLLIIAIYLVGIFLIGLKYSRNYRGNVDDYLLAGR
                     RLSLPAFVATLVSTWYGGILGVGEFTYRYGLLNWVTQGLPYYFFAILFGIFLAPRIQR
                     SGLYTIPDQLYQRYGKPAGFLGSVFVFLLTNPASHLLMSTLLLSAIFGISFWLAFLIG
                     VIFSLFYVFMGGMKSDVLTDFFQFGLMFLGFGVLAVKLITNYGGLNFLQANLPQNHLR
                     FTGGENWQYIMVWFFIAFWTFVDPGFYQKCYAARTPQIARRGVLISVIFWLLFDMLTT
                     TCGLYARALIPDTNPLMAFPKLGEMALGDFWRGIFFIGLLATIMSTLDSVALLSAMTF
                     GRDLLWRLKPNSNINRNTQIGFFVIIGLSILVILFLPSVVKIWYTFGTLFIPALLIPV
                     ICNFTKYNYKSSVMFTGMLGSFFATLIWFIIGMRNGSIMEPIYLFNIQPFFIGLLSWL
                     LFLPFLVDRFTT"
     gene            12054..12127
                     /locus_tag="PLO58_03915"
     tRNA            12054..12127
                     /locus_tag="PLO58_03915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12088..12090,aa:Val,seq:cac)
     gene            12236..13036
                     /locus_tag="PLO58_03920"
     CDS             12236..13036
                     /locus_tag="PLO58_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HQO73971.1"
                     /translation="MFKELERLVEVQKIDIKLLEINNLRGDLPQIVENLQAQIDTLSK
                     ELENERKREQEITREIKELESKIADDRILLNRYQDQLYLVTSNKEYDALTSEIDNLKQ
                     EIDNAEYNILKLSDEIERLKISIKSKELTLEEKTRELDQKQSLLDKTDITTKDAQEAL
                     LEQRKRIVDLIPIRYMREYERIAKAKNGIAIVPILQIFEEKVDKKGNVEYIPMQVSCG
                     GCSKIVPPQKILEIRSGDRINRCEFCGRILYWDEQSSEIRSNYEEELF"
     gene            13035..13422
                     /gene="rnpB"
                     /locus_tag="PLO58_03925"
     ncRNA           13035..13422
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLO58_03925"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            13433..15139
                     /gene="recJ"
                     /locus_tag="PLO58_03930"
     CDS             13433..15139
                     /gene="recJ"
                     /locus_tag="PLO58_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQO73972.1"
                     /translation="MKQKWLISNPNPNAVHQLAGELQIPPAIVRILLNRGLFDKDSIE
                     KFFSPSTQDTHDPFLMADMTKAVERLGQAILRREKVLIFGDYDVDGTTATSLLYLVFH
                     EIGLDTAFYIPDREREGYGLSQLGIEYARQIGATLIITCDCGINAFDTIDYANSNQID
                     VIVTDHHEPAEKLPAALAILDPKREDDHYPFKELSGVGVAFKLLQGFHIANNLPVTNL
                     YRHLDLVAIGTAADIVPILGENRVLVAKGLEYLNSSQKVGLQALLKTSGFDSKTMNVV
                     NIVFGLAPRLNAAGRLGEASRAVHLLTSFDRKEAFELANLLEQENRNRQAIERETIDS
                     AILQLNATHDLDADKILILSNTNWHHGVIGIVASKLKELYNRPVVMISFQNGIGKGSA
                     RSIPGFDLYSAFNSCADLLENYGGHKMAAGLTILIESLPEFTRRLKKIAAEQIDEQML
                     NSGLQIESEIDFNDINPQTLSFLHKMAPFGPGNMRPVFCTRNVTVSGMPRIISENHLK
                     FKAAQNRSVLSAIGWKMGCYYEMLISNRPLDIAFVIDENEWNGYKEIQLNIKDIVYSD
                     YH"
     gene            15164..16156
                     /locus_tag="PLO58_03935"
     CDS             15164..16156
                     /locus_tag="PLO58_03935"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQO73973.1"
                     /translation="MYKSKIASLGKNVPENIVTNADLEKLMDTSDEWIVERTGIRERR
                     FVTPPCGASQLALPAVLEAITNAGLTKNDIDLIIVATTTPEHLTPGTSSYLQALLELP
                     NIPTLDIRVQCSGFIYGLAIADAFIKTGQYKNILLVGVEVQSVGLNLTTVGRDIAVLF
                     GDGAGAAVITRSTDESAILSTHLFGDGRYANELWVEAPDGSKRPRLSKEMLDEGKHYP
                     KMNGRQVFKHAVTKFPEVINIALEANNLNKSDITLIIPHQANLRITEAVAQRLEIPMS
                     QVYSNIHKYGNTTAASIPLAMYDALQEGRYKKGDYLILAAFGSGFTWASAALRW"
     gene            16185..>17221
                     /locus_tag="PLO58_03940"
     CDS             16185..>17221
                     /locus_tag="PLO58_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73974.1"
                     /translation="MANSNSSKIAGKERGVFQSRRQRTGVFLICGVIVVALALLFPKT
                     HYNITKYKINDIARSSIIAPFDFPILKTEQELEADRLEAIKKVPFVFIQDKDVEKTEL
                     MNLQKFFTEIDRLRLARIKYDESLRNISINRSEESLAILTADSITFFALQKKFLNDYK
                     LNKNSPVLKSLVYQYDPKNVNQLSAHVQNQVKSVLSELYVLQILDIPKSQIISEKIAI
                     LQGGEEVLENPNEVLELSEAWTKAKLALQARLNDYTPEVVNAAYDIVVAFLKPNLIYQ
                     KQITEKRQAEAIAKVPISKGVVLKNEKIVDANTRITPEIYQKIESMAKEQARLANVQR
                     SWRRALPVIGDP"
     assembly_gap    17222..17321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17322..18392
                     /locus_tag="PLO58_03945"
     CDS             <17322..18392
                     /locus_tag="PLO58_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQO73975.1"
                     /translation="AQIGGNFNFIIVSVFVSTFAIYAVRQLRKRSQIFKSIIYIVLGY
                     CLAIFATEMLKYSPPKSILNNIFYATINGVFAPFLAYGIIGLLENLFGITTDLSLLEL
                     ADFNQPLLKLLSHEATGTFTHSVTVGNLAEAAADAIGANALLARVGSYYHDIGKLTKP
                     EYFIENQAFDYNRHDKLAPNLSAIIILNHVKEGLRLANEYKLPQVIQDFISTHHGTTR
                     VEYFYDKALKKSAKPDEINDADFRYPGPKPHTKETGIVMICETIEAVCRSLNKPTMSA
                     IEKVVDSVIESRLKEGQLDECPLTLADLKKIKGDLKEGTGILPILRGIHHLRVEYPGQ
                     EKFSNSSVPSNKKGKNDDSQNL"
     gene            18370..18834
                     /gene="ybeY"
                     /locus_tag="PLO58_03950"
     CDS             18370..18834
                     /gene="ybeY"
                     /locus_tag="PLO58_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQO73976.1"
                     /translation="MTIVKIFNNHSHYTLLKPAIEQVVSYIVNMEQQAVQSITFIASD
                     DKTLNEMKVKLFGIDQLTDTISINYNEIDQPVEGEIYLSLDRIEDNARTFKVTFEKEL
                     ALVIIHSVLHLLGYNDDNLNDKKQMQALQNFYIQQIKFPRLYRQRRIQPNYE"
     gene            18827..19606
                     /gene="mazG"
                     /locus_tag="PLO58_03955"
     CDS             18827..19606
                     /gene="mazG"
                     /locus_tag="PLO58_03955"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQO73977.1"
                     /translation="MNDELSRYFDELIEIVKTLRGPNGCEWDKAQTRDSLTPYLLEEA
                     HELIDSISENNPSKIKEELGDVLLHIIFQAQIAAEAGEFTIADALQQINAKLIRRHPH
                     VFGETKVENVNDIKKNWEEIKLKEGRQSLMDGLPKSLPALLQARRVQERAAQVGFDWS
                     TIEPVWAKITEETRELQEAIATRKKDEIVAEFGDLLFTMVNLSRFLDIDPEEALRQTV
                     TKFIRRFQAIENELAATGKNFKDTSLEELDKIWNKSKDKES"
     gene            complement(19908..20519)
                     /locus_tag="PLO58_03960"
     CDS             complement(19908..20519)
                     /locus_tag="PLO58_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HQO73978.1"
                     /translation="MAEMLPQELKTIADSAEIMLTVKDSKFIGYCFPVADKSEAVQKI
                     AEISQKHRKATHICWAYRIYENNQLLYYAHDAGEPRGSAGAPILKALTSRQLVKALVV
                     VVRYFGGTKLGIGGLIRAYGATAGKTLDLVEHKPFIQRQTLRIECPLANYSQLAHFLG
                     RFSESSKPEFTENNVIVTVQIEVQKADEFRTELNSIPGLIITT"
     gene            complement(20531..>21883)
                     /locus_tag="PLO58_03965"
     CDS             complement(20531..>21883)
                     /locus_tag="PLO58_03965"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO73979.1"
                     /translation="AAERSYSLLIDTLGFQSPPFDDIFSPETDIYIINLGGAAYAYTY
                     DENPVQTIPDRPYDYTAYTVIDNDFVGYPTTGLNGLRVTVAHEFFHVVQLGYNWWEGN
                     GLANSGGGRTSADDYFLEWCSTWFEERAYPEVNDYLFYVGAFFNSPLKSLWNYDYAYA
                     LGAFLRFMLDRYGENLLVKVWEEIKTNYAFESLQAVLADDYHADLADLWNEFYLRCYF
                     TGERYDPDWALSSDAMQFPLLQIPATNKVVFTESAVFTPSIEPFACVPYQVVFDQNWL
                     CGINVNSSSPENFLGRYLFIKNRVGKIAETLSLNQNLLVGNAANNDSLLIFMTNSSKT
                     TTPNLTVTISEVGDEFRIASTIKNVYPNPYSPGKSGDLIVDLQVGQIVNSIKTNWFDL
                     TGRNVFQKNFDNGLIEIGNYSLSFSSDELRQAQLASGVYIMQIAIGSKNLTRKILLLK
                     "
     assembly_gap    21884..21983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22027..23340)
                     /gene="der"
                     /locus_tag="PLO58_03970"
     CDS             complement(22027..23340)
                     /gene="der"
                     /locus_tag="PLO58_03970"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQO73980.1"
                     /translation="MAEPIVAIVGRPNVGKSTLFNRLTQKRKSIVHSEAGITRDRVYE
                     TVNWAGRQFLLIDTGGFIPESTNQITKAIREQVRLAIEEANLVIFVVDASEPMTALDR
                     EIAEMIRRSQRDCLIVANKCDNERLEVNSLEFTELGLGDVFPISALNGRHIGDLLDAI
                     LQRIPRKIAANDDEKDHLQLAIVGMPNVGKSSILNVLAGSEKAIVTDVPGTTRDAIDT
                     KIKYFNEPVTLIDTAGMRKHRQIKDEVEYYSLLRAYRAIERCNVALVVVDAVKGFTRQ
                     DAAIVRQVIDLRKGLVIAINKWDLVQRETNTQVNFKRNLIEQFRELEHYPIIFISAKT
                     HQRVHKILETARMVYENRKIRIGTSELNQFLQDVIDRTPPPANLGKFIKIKYITQVKT
                     EPPVFVFFCNEPRLIKEEYRRFLENQLRRRFGFSGVPLIISFRNK"
     gene            complement(23333..24619)
                     /locus_tag="PLO58_03975"
     CDS             complement(23333..24619)
                     /locus_tag="PLO58_03975"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HQO73981.1"
                     /translation="MVEIVGIKPNSLAEMNGIRRGDQIVSINRQPINDFLDLYFHQSD
                     ENCKVVVLRDGHKKTFQIAKNADEPLGIELSPPRLKACGNNCIFCFIKQNPPGMRPAI
                     YFCDEDYRYSFLLGNYITLTNLSEADLNRIVLQRLSPLYVSIHATDENVRRHIFQLKK
                     PDNLMQKLTFLAQNRIDLHTQIVLIPGVNDGAVLEKTLADLYIFRDSILSVAIVPVGL
                     TAHRQHLPVIPAVDSHLASQLLENIRTWNRCYVNRDGEHWVFLSDEFYLLVGQDLPGC
                     KFYGSFYQIENGVGTTRAFLDDFKRQSRKLPHAISKRKRVLFVTGMLAAPVLSRYVTA
                     RLNQINNFSVDILPVINNFFGPSVTVAGLLTGQDILAQAINVDEYDLIVLPPRIVNED
                     GILLDDLYPYQIAEALRKPVQVWDGDFGKLVDEDNG"
BASE COUNT         7060 a         5530 c         5023 g         6703 t
ORIGIN      
        1 gagtgctttc ggtcaacagc ttgcggccgg ttcgtccggt agtggaaatt ctaattgata
       61 gcgacggcaa tagattaaaa aatccgattc ggctcgatct cgaggcagag ccattaattt
      121 atatagctaa gccgctcgcc gaatcctgag cgattcccct cattttcaaa gtgcctgact
      181 cagccacaat ctatgcggtt ggacaggcat tttttatttt tttaaaagga tttttggtac
      241 agtacgccag attcgatgag aaatcaaagc ggattgttat tggcatcgag cctaattatc
      301 gtgggatgcc aatcgcctaa ttcagccggt gatacaaccg caaaagccag aataataatg
      361 cgatcgccct cctcgaccaa gcgagcggca gcaccattca aactaatgtc tcgctgaccg
      421 gctttgccct caatcacgta ggtttcgaag cgagctcccg agtttaaatt taaaaccagc
      481 actttttcga aaacatgcaa atttgccaag cgaatcaatt ccgcatcaat ttcgatacta
      541 ccctcatatt ccaagttcgt ggcagtaact cgggcattgt gtattttaga ttttagcatt
      601 atcaaactac tcataattca ttcctataat caaactcatt tccaaaacaa atttaccaat
      661 taaagttact ttattcaaat ttcatgtttt cttacttggt tgttcaaata taaatttgag
      721 tggttttaat atcctatttt gttaccattt taattgacgt ttaatgaatt tttaatgaag
      781 gtttagattt acttgccgag tttgatttac cagttggatc ttaaattgtt tcgtctcatc
      841 aatcaggaac tgagcaatcc cgtattcgat tggtttatgc cctttatcag cagtaaatct
      901 ttttggttga ttccaatttt catcggatta gttctccttt tctggctggg gaaaaaaaga
      961 ggacggatcg ccacactttt aataattctt actattgcga ccgcggatcc gttctgcgcc
     1021 cgagtactaa aaccgacctt caaacgctta cgtccctcgc acgaactcag cgacgtccgc
     1081 ttattgggta aaaagggtgg caaatacgga ttcccatcca atcatgccgc caatgttgcc
     1141 ggttcaatgc taattctggc ttttttctat cgccgatata aatacatctg tggcgtgctg
     1201 gcatttctag tcggctatag ccgaatctat ctgggagtgc attatccgac ggatgtgctg
     1261 gcgggattcg tgatcgggat tggtatatcg gtaggttgga ttctcatttg gcgctcctta
     1321 accaaccatt tattgaaaaa aagaaacgat ttattggctt taagttagga ttttgaattg
     1381 gcataagcag tatataaaaa ttttagaatt ttcgaattcc tatcatnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnngaaa ggattcattt atgtgtggca ttgtggctta
     1561 tatcggtaat caatcagccg ttccgttatt attggaggga cttaaaaaac tcgagtatcg
     1621 cgggtacgac agcgctggaa tttgcatcgc tggcgaccat cgcctggaag tggttaaaag
     1681 gagcggcaaa attgccgaac tggaaaaaac ggccgctctc gattcgtttt cttcccatat
     1741 cggtatcgcc catacccgtt gggcaactca tggcgctcct aatgaaatca acgctcatcc
     1801 ccacctctcc tgccatggtg aaattgccat agtccacaac ggtataattg aaaattttcg
     1861 tagtctaaga gatatgttaa tccaagaagg gcatcatttc tcgtccgaaa ctgattcaga
     1921 agtattggct catttaattg aaaaatttta tgaaggcgac ctagcaacag ccgtcgttaa
     1981 agccttgcat aatgtcgagg gtactttcgg aatagcagtg atgcataaag atgaggaaca
     2041 tttggtaatc gctcgccgtg gctcaccgat tattatcggt ttaggtaatc aggaaactat
     2101 tgtagcttcc gacgccgttt ctatcattcc tcatactaaa aaagtcatct acttgaatga
     2161 caatgaaatc gccgtgatcg gatataacaa ctatcgtttg caaaacctcg acggtcagtc
     2221 catagccaac gagatccaag aaatccgctg gaatatttct cagatcgaaa aacgcggctt
     2281 taaacatttc atgctgaaag aaatatttga gcaaccggaa gccgttcaga acactttgcg
     2341 cggtcgcatc actaacggac aaatcaaatt atctctcaac actgatttta atcgcgtgca
     2401 aaggatcatt attgcggctt gcggcactag ctggcattca gccttgattg gtaagaattt
     2461 aatcgaaaaa tatactggca taccgactga agtagattat gcatccgagt ttcgttaccg
     2521 cgagtctata attaacggcg aagttctgat gatcgtcatt tctcaatcgg gcgaaaccgc
     2581 cgatactttg gcggctttac gaaaagccaa atcagcaggt acgcagacac ttggcattat
     2641 caacgtggtc ggttctacta tcgcgcgcga agtaaattcc ggcatttact tacacgccgg
     2701 accggaaatc ggcgtcgcca gtaccaaagc ttttatttgc caggtgattg ccttacttct
     2761 actggcactc tatatcaagc aacaaaaagg agaactcatt gatccggctc ttttagccaa
     2821 gatagatatg attccggaac aaatccgcca ggtagccgaa cattctgaca atctgcggga
     2881 aattgcatcg gcttattatc tgaatcgcaa ttttatctat cttggacgtg gtatcaatta
     2941 tcccgtcgct ttggaaggcg cattgaaact caaagaaatt tcatacattc atgccgaagg
     3001 ttacccagcc gccgaaatga aacatggacc aattgctttg attgacgaaa atatgccagt
     3061 cgtttttatc gctacgaagg gggctgtttt cgataaagtt ctcagcaata tgcaggaagt
     3121 caaagcccgc ggcgggcaaa ttttaaccat taccgaccat aataatccac atctgcaaga
     3181 actctccaat catctgatag ttgttccgga gaccagcgaa gacctttcgc cgctcatcaa
     3241 tgtcataccg ctccaattac tagcctacca catcgctgat ctgaaaggct taaacgttga
     3301 taaacctcgt aatctggcaa aatcggtcac agttgagtag ctgaaataga aataaaactt
     3361 agctaacaag gagcgttagc atttgcctcg tctcgaaaaa aactgcaaca attctccttt
     3421 taatttctat acttttttta aattaactga gttttaagat agcggagaaa attatgttaa
     3481 aagaaataag tctgggaata attttaatgt tcagcctgtg ggtaaccggt cagacggctg
     3541 agccgttttt tccttataaa tataatgtgg aagttttacc aaacggcttg aaagtcatta
     3601 ctattccaat ggaaagcaac gggatcgtgg cttattattc catcacccgc accggcagtc
     3661 gggacgaatg ggaaccggga cattccggat tcgcccattt gtttgaacat ctgatgttcc
     3721 gcggcactga gaaatatccc ggctcgcttt atgataaaac tataactgaa ttaggcgccg
     3781 acgctaatgc ttataccacc gacgattaca cctgttatca tctgaacttt gccgcggaag
     3841 acctggaaaa aatcatcgaa atggaaagcg atcgcttcat caatttagct tataaggagg
     3901 aagaatttaa gaccgaaacc ggagccgttt acggtgaatt tctcaaaggg cgagtcagtc
     3961 cgtggttcgt cttgtacgaa aagctttacg atgtcgcttt cgacaagcac acctacaaac
     4021 acactaccat tggatttgaa gctgatgtga aagctatgcc tacaatgtat aaatacagta
     4081 aaactttttt ccaacgttac taccgaccgg agaatgtcgt tctgctcatc gtcggtcaga
     4141 tcgagaccga gaaaactctc gaacttgttc gtaaatatta cggcgattgg caacccggct
     4201 atgtctctcc ccaaatcgaa cccgaaccgc ctcaaactgc cgagcgcaag gcggaagtaa
     4261 tttatcccgg gaaaaccctg ccaatcgtca tggtcgctta taaaagtgat gcctatgatc
     4321 ctaataatcg tctatacgta gccggacttc tgctagagca attagctttc ggagaaaata
     4381 gcgaccttta taagcgattg gttttaaaag aacagaaagt cgagttcatc acagccgact
     4441 tttcggtcag ccgcgatcct aaattgaacg aaatttacgc tatggtaaaa tcgccggacg
     4501 atattgatta cgtccgcgaa cagattttta tgacaatgga acaattcaag accgaagccg
     4561 tccctgagtc tcgcctcgag gatctcaaaa aacatttgaa atacagcttt ctgatgaatc
     4621 tggacactcc tgataacgtg gcgggcaatc tagctaatta cgttgccatt acaggtggag
     4681 ttgaaaccgt tgatcaacta tttaaccaaa taatgaatgt cacggtcacc gatattcagc
     4741 aagccgctca gtattatggc gttcccgaaa aaagaacagt agtcattctt aaaggagtcg
     4801 cgcaatgaaa accaaattaa ctctactatt aagttgggga attatggcta gcattttact
     4861 ttcaaattgt ggttctaaaa tcgacgcttc tcgttgtgtg ctcctaccgg tagccgaaga
     4921 tccgactgtc tcttaccgca tttatttcaa agtcggctcc caggatgatc ccgccggtaa
     4981 agagggattg gcgttcctga ctgctcatat gttaagtgaa gccgctactc aaagtaattc
     5041 ttacgaagaa ataattgcga aattatttcc actagcggcg tcctacgacg ctacggtttc
     5101 ggcagagatg actgtgattt ccggccgcgt gcaccgagac aatctcccgg cttattaccc
     5161 gctttttacc gaagcgatcc tcaaaccggc tttccaagaa gatgacctga atcgtctgaa
     5221 aagcaacgtc ttgaattatc ttgaaaatgt tttgaaatac gctaatgacg aagaactcgg
     5281 caaggcggtg ctttataatg aaatcttcgc cggaaccggc tacggacatc cgaccgaggg
     5341 attaatcaac agcattaatt ccattacgat caacgacgtc aaagatttct atcaaaaaat
     5401 gtataaccgt aacaatgtta ttattggtgt aggtggcgga tatgataacg aaacattggc
     5461 aaaattgcag aaagacctgg cgcaacttcc tcgcggcgtg gaaaatatcg caactcgagc
     5521 cgttccctac gacattcagg gcttaaaagt taccatcgtc gaaaaggacg ctaatgctac
     5581 tgccattagt cttggttttc caatcaatat cacacgggaa aatccagaat ggtatgccct
     5641 ggcagtcgcc aattcctggt tcggcgagca ccgcaattcc agtagtcatc tctaccaagt
     5701 catccgcgaa acccgcggct tgaattatgg cgattacagc tatatcgaac attttcccaa
     5761 tagcggctat taccaatttc caccggtcaa cgtcgcccgc catcagcaaa tattcgaaat
     5821 ttggattcgg ccggtaccga acgaaacccg ccatttcgtc ctaagagcaa ctatgcgtga
     5881 attacataat ttcattgaaa acggactaac gccagagcaa ttcaatctaa cccgcaattt
     5941 cctacgcaag tatattctgc attatgcccc gacgactacg atgcgcctcg gctatgcaat
     6001 tgatgacaaa ttctatggaa taaaaggaag tcatctggaa aatttccgca tcgcactcga
     6061 caatatgacc gttaacgacg tcaatagcgc catccgcaaa tatctgcaat atgataatct
     6121 gcaaatcgca atcgtcacca acgaagccga aaaattacgc gaagacttgc ttgctaatac
     6181 acccagcccg attaaatatg ttaccccgaa gcccgatgta gtgcttaaag aagacctcga
     6241 gattgcaaac tacccacttg ctatccaacc cgaaaatatt aaaatcgtaa acattcggga
     6301 tttattctat gaataattcc gccaaaaatt tcacagattc acgcactaaa gatgtcaagc
     6361 aatgcttcga catctcgacc gaattgcgac tcgataataa atactatcgc aaattcgtac
     6421 tgactttttt cgacaatcta ctggattttg acatcagcag taaggacgca tctttacaac
     6481 cagcgaaata ttatcggcgc caggtaaccg ccgttatcaa tgccaaagcg ccggcaatta
     6541 ttgccggaat ggcggaaata caggcttttc tcgatcatca taatctacnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tatttctata tcaaccataa ttaattttgc
     6721 tccgatgaaa ccgatttatc ttgattataa cgctagcacc cctctcctac cggaagtggt
     6781 ggacgctatg ttaccatttc tgaaaaatgt cttcggcaat ccatccagca gtcatttcta
     6841 cggacaggaa agcaaacagg ccatcgaaaa tgctcgccgt caggtcgcgg aaatgttgaa
     6901 ttgccagcct gaagaaatcg tcttcaccag cggcggcagt gaatccaata acttcgctat
     6961 tcgcggaata gccgaaatgc accccggcgg caacatcatt accagcacta ttgaacaccc
     7021 agcagtcctg gaagtctgtc gctacctgga aagtaaaaat tactccgtta cttacttacc
     7081 ggtcaatagc gatggtttgg tcgaattgcc tgccgttcaa gccgccattc gtcccgacac
     7141 tttcctaatt actattatgc ttgccaataa cgaaatcggt acgattcagc cgattcgcga
     7201 aatcgccgct ctcgcccata aacacggaat tttagtccat accgatgccg ctcaggcggt
     7261 cgggaaaata gtcgttgatg tgcgcgagct ggatgtggat ttactctcag tcgctggaca
     7321 taaaatgtac gctcccaaag gtgtcggtgc ccttttcata cgaaccggca ctcaaatcgc
     7381 tccattgatt tatggcgccg gacatgaacg tggtttgcga cccggcaccg aaaatgtgct
     7441 ggaaatcgtc ggcttgggta aagcggctga aatattccga gcgaaatcgg ctgaaattgt
     7501 gtcccgacaa aaggcgcttg gtgaaaaatt cttcaacgat ttacgaactg caattcccga
     7561 acttggctta aacggtcatc caacgcaaaa actcaccaat actttcaatg tttattttcc
     7621 gggaatagat gccaatacca tgctggcgga attgacgcaa gttgcggctt cggctggcgc
     7681 cgcctgccat gccagtagca ttacgccttc gcatgtttta aaagcgcttg gcttgccgaa
     7741 tgaacggatt ctcggaagtg tccgcttctc gctcggacgt tttacgaccg aagatgaaat
     7801 cgcggatgct gtatcggcaa tcagtcaaac ttaccatcgt ctaactagca ctgaggaaat
     7861 ttctatccca atcacatcta acgaaccaat ccggttaacg caattcacgc acggtctcgg
     7921 ttgcgcctgc aaaattcgtc cccaaactct cgaaaaaatt ctacgtgaaa tcagaccacg
     7981 catggcactt aacgtgttag ttgacttctc agcttccgac gacgccgcca tttataaaat
     8041 taatgacgag caagcgatcg tagcaaccgt tgattttttt actccaattg tggatgatcc
     8101 atacgatttc gggagaattt cagcggctaa ctcgttgagt gacatttatg cgatgggcgc
     8161 tgagccactg tttgccttaa atgtagtagc cttccctgaa aaacgcctgc cggtagaaat
     8221 tttgactcag attttgcgcg gcgcacaggt tatcgctgat caggctggta tcccgattct
     8281 cggcggtcat accgtcgaag ataacgaacc aaaattcggt ctggtggcaa ttggcattat
     8341 tcatcctgaa aaaattatcc gaaatatcgg cgcgcaaccc ggtgatgctt taattctgac
     8401 caaaccgatc ggcgtcggga ttctttcgac ggctctcaag cgcggtttat tagatgaact
     8461 gacgaaaaat ctgatcatcg ccaatatgac cgaactgaac gccaccgccg cagaagtgat
     8521 gaagtcattt ccggtgcacg cctgcactga cattaccgga tttggtcttt tgggacattt
     8581 acatgaaatg agcaccggca gtcgtgtccg agcccagatt tcagcctcgt ctgtgccgat
     8641 tttaccgtcc actaacgaga tggttttacg caatgttatt ccgggcggca ccgaggcgaa
     8701 cttatcgttt atttcaccga tggtcatctg ggataaaatg ataacggata gcgataaaat
     8761 aattttgtgc gatgctcaga cttctggtgg cttgctaatt gctgttgcgg atgaatttgc
     8821 caatgatatg ctggcacaat tacatcaagc aggtgtaata aatgcggcta aaatcggcaa
     8881 aattgagtcc ggtgctgatg gaaaaattaa ggttgttcct tagtaaaaat tttttagatt
     8941 agcaaagtta tgacccgttt tcgtaaagaa tactcaaaaa ttgcccggaa gctgacaagc
     9001 gccgaccttc aatccggaat aggaaacagt gaattaagtc tcgataatat tgttagtgat
     9061 ggtagattag ctgtggactc gcaatttttc atgggcgttt tcagccgaca gggtttgcta
     9121 aatgccattc gggctttcgg acttttggat attctcgagc gaaaaggatt gacgaatatt
     9181 acaatagaca ttgacaccag tgatccgtat atccatcgcc tttatgccta tagcggaaag
     9241 ccgctaaacg aaaataaaat ctgtgaatta gtattaaaac aaggtccgct tcattttgaa
     9301 aataagccac tatcgtcttt cccccgtaga actgccaata tattatatat agaatggctt
     9361 ctactccaaa atccacgcga aaaattttca cccgaaaaac cgcccttgcc cggccagaat
     9421 tatcccggcc ttggtattgg cgatcgccta atggaaatta tgatacttat gacccgtcgc
     9481 cttaacttgg aaggtattat caataaaccg cattactttc atacagcgtt tatgttcagt
     9541 aaagagttcg tgttcatcaa tccctataat caggctttga tgtatgcaat cagtaaagac
     9601 ttattggcta aatactcatt ctggatggta gcttgggcgg cgcatttcaa ctgtattatt
     9661 aataaaaatg acggcaaacc aatggattgg aagacaggag accttatcct gcccttcacc
     9721 aaagatttga ttaaatactt tcgcgggcgc gaatatcagc aggaggttag ccgacttata
     9781 gctaatttct ccttcgaaat cgatcttgat aaattacata ccgcccttaa aagcaatgac
     9841 cttgaaaaat acgctcgatt tcctaaataa ttttctgaat taaattgact cccaacacac
     9901 caataaattt tctatcaccg acggctttag tcctgggaaa tggaaattac ccaaactggt
     9961 ttgatatcaa acaatttcaa gtggaatata aagtgctatt ggtttgcgcc gacggtggag
    10021 cggaaaaagc acgccaattg aatttaatgc ccgatttgat aattggcgat atggattctg
    10081 ttaccacgga taccataaat cattttcaat ccagcgaggt aaagatcatt caccaaccaa
    10141 atcagaataa caatgatttg gaaaaatgcc tgcgctggct tttgcgacat ggctggcggc
    10201 aattcgtatt agttggattt accggaaggc gcgatgatca aactatcgca actttacaga
    10261 taatgagaaa gtatgcccgg cgagcgcagt ttctgttata taccgagaca gcgcaaattt
    10321 atttattgaa aaaaggcatc tggctcatta acgccatccc cggtcagacc atttctctgt
    10381 tcggttttcc aagagtttat catctgacta ctcggggcct tcaatatcca accaaggacg
    10441 aaaatttaac aggtggttcg cacggtctaa gtaataccgc cattgatgaa atggttcaaa
    10501 ttcaattttc cagtggttat ttattagttg tcgcgattca ggagctaaat tgacgtctct
    10561 tagattggct ccgattgatc tgttaattat cgccatttat ctggtcggca tatttctaat
    10621 tggattaaaa tactcccgga attatcgagg aaatgttgac gactatttat tagctgggcg
    10681 caggctttca ttaccggcgt tcgtcgccac tctggtgtca acctggtatg gcggcatcct
    10741 gggggtcggt gaattcactt accgttacgg attattgaat tgggtgactc agggattgcc
    10801 gtattacttc tttgctattc tttttggaat ttttctagcg cctcggattc aacgtagtgg
    10861 tctttacacg attcccgatc agctttacca gcgttatggc aaaccggctg gatttctggg
    10921 tagtgtgttt gtttttttgt tgactaatcc cgcttcgcat ttattgatga gtacgctttt
    10981 acttagcgct attttcggaa tttctttttg gttggctttt ctgattggag ttattttttc
    11041 cttattctat gtttttatgg gcggaatgaa atctgatgtt ttgacggatt tctttcaatt
    11101 tgggctaatg tttttaggat ttggagtgtt ggcagtaaaa ctcataacta actatggcgg
    11161 gttgaatttc ctgcaggcaa atctgccgca aaatcacctc agattcactg gtggcgaaaa
    11221 ctggcagtat attatggttt ggttttttat cgccttttgg acttttgttg acccgggttt
    11281 ttatcagaag tgttatgccg ctcgcacgcc gcagatagcg cgccgcggag tattgatttc
    11341 agtgattttc tggttgcttt ttgatatgct cacgacaacc tgcggtttat atgcgcgggc
    11401 tctcattcct gatacaaatc ccttaatggc tttccccaag ttaggcgaaa tggcactggg
    11461 agatttttgg cgaggtatat tttttattgg tttgctggca acgattatgt ccactttgga
    11521 ttctgttgcc ctgctctcag ctatgacttt tggacgggac cttttgtggc gtctgaaacc
    11581 caatagcaat attaaccgca acacccaaat cggattcttt gttatcattg gcttatctat
    11641 attggttatt cttttccttc cttcggtagt aaaaatctgg tacacttttg ggacattatt
    11701 tatcccagct ctgttaatcc ctgttatttg taattttacg aagtataatt acaagtcatc
    11761 cgttatgttc accggcatgc tgggatcgtt tttcgcaaca ttaatctggt tcataattgg
    11821 aatgagaaat ggttcaataa tggaacccat ttatcttttc aacatacagc ccttttttat
    11881 tggattgttg tcctggttac tctttttgcc ctttttagtt gataggttta ctacctaaac
    11941 tgaccgacat aataattcaa taaactgaaa aaatattggt gcaaatctgt tgtattaatc
    12001 agtcagatta gctaaatttt gttgcttttt taaaatgtcc ggttaattta actgggcgat
    12061 tagctcagtt ggtcagagcg tcccgttcac atcggggagg ccatccgttc aagtcggata
    12121 tcgcccacgt aataggttgg gttttgaaag aattaattgt ccgatacaat acattacacc
    12181 atataaaatg caccgcttgc ggtgcatttt ttttataaaa acaactggag gttaaatgtt
    12241 taaagaactc gaaagattgg ttgaagttca aaaaatcgat atcaaattgc tcgaaatcaa
    12301 taatcttaga ggcgatttgc cgcaaattgt cgagaatctc caagcccaaa tagatacctt
    12361 atccaaagag ctggaaaacg aacgtaagcg cgaacaagaa attacgcggg agataaaaga
    12421 attagaaagt aaaattgccg atgatcgcat tcttttaaat agatatcaag atcaacttta
    12481 cttagtcact tctaataaag aatatgatgc tctgacttcc gaaattgaca acctcaagca
    12541 agagattgac aatgccgaat acaacatctt gaaactttcc gatgaaatcg aacgcctgaa
    12601 gatttcgatt aaaagtaaag aactaaccct tgaggaaaaa accagggaat tggatcaaaa
    12661 acagtcccta ttggacaaaa ctgatattac tactaaagac gctcaggagg ctttactgga
    12721 acaaaggaag cggattgtag atttaatccc aatccggtat atgcgagaat acgaacgtat
    12781 tgctaaagcc aaaaacggaa ttgcgattgt cccaattctg cagatttttg aagaaaaagt
    12841 agataaaaag ggaaatgttg aatatattcc aatgcaggtt tcctgtggcg gatgttcgaa
    12901 aatagtgccg ccacagaaga tccttgaaat acgaagtggt gaccgaatta atcgctgtga
    12961 attctgcggc cgcattttat actgggatga gcaaagcagt gaaattcgtt ccaattatga
    13021 agaggagctt ttctgaagtt ggtcagacag ccgctccgat tttatcggag aggaaagtcc
    13081 gaactccgca gggcagggtg tcccgtaatc cgggatcatt ccgaatgatc ggaatgcagg
    13141 aaagtgccac agaaaatata ccgtcccgcc ccggcgggat aagggtgaaa aggtgcggcc
    13201 cgactgaggt cgggattaaa gtaagagcgc accgtcccgt ctgcaaaggc gggagccagg
    13261 caaaccccac ccggagcaag agcaagcagg gaaaaggaat agcccgttcc gtttgattcc
    13321 cgggtagctc gcttaatccc gactgaaaag tcgtggatag ataaatggct gtcgtctcga
    13381 ttcgtcggga tacagaattc ggcttataga ccaacttcag aaattttata tcatgaaaca
    13441 aaaatggctt atcagcaatc cgaatcccaa cgctgtccac caactggcag gtgagctcca
    13501 gataccaccg gctattgtgc ggattttact taatcgcggt ctgtttgata aggattcaat
    13561 tgaaaaattt tttagtcctt ctactcaaga tacccatgat ccctttttaa tggcggatat
    13621 gaccaaagcc gttgaacgac tcggtcaagc cattctacga cgcgaaaaag tcttgatttt
    13681 cggcgattac gacgtggatg gcaccacagc tacttcactt ctttacctgg ttttccacga
    13741 aatagggctt gacaccgcat tttatatacc cgacagagaa cgggaaggat atggtctctc
    13801 ccaactcggc atcgaatatg ccagacaaat cggagcaact ttgattatca cctgtgattg
    13861 cggaatcaac gccttcgata caattgatta tgctaattcc aaccagattg atgtgatcgt
    13921 taccgatcat cacgaaccag ctgaaaagct tcccgccgcc ttggctattc tagatccaaa
    13981 gcgcgaagat gaccattatc catttaaaga attatccgga gttggcgtcg ctttcaaact
    14041 attacaggga tttcatattg caaataatct accagtcacc aacctttatc gtcatcttga
    14101 tttagtggca atcggaactg ccgccgacat cgttccaatc ctcggcgaaa accgtgtttt
    14161 agtcgccaaa ggtttggaat atctcaacag ttcccaaaaa gttggcttac aagctcttct
    14221 caaaacctcc ggcttcgata gcaaaacaat gaacgttgtc aatatcgtct tcggtcttgc
    14281 ccccagactt aatgccgccg gacgactcgg ggaagcctct cgagcagtac acttgctgac
    14341 atccttcgac cgtaaagaag catttgagct agccaacctc cttgaacagg aaaaccgcaa
    14401 tcgccaagct atcgaacgtg agacgatcga cagcgctatt ttacaactta acgccactca
    14461 tgatttagat gccgacaaga ttctgattct gagcaacaca aattggcatc atggtgtcat
    14521 cggtatcgtc gcctcaaaat taaaggaatt atacaaccga ccggttgtca tgatctcgtt
    14581 ccagaatgga atcggcaagg gctctgcccg cagtattccc ggctttgatc tatatagcgc
    14641 cttcaactcc tgcgctgact tgctggaaaa ttacggcgga cataaaatgg cggccgggct
    14701 gaccattttg atcgaatcac taccggaatt caccagacgc ttgaaaaaaa tcgccgcaga
    14761 acagattgac gaacagatgc ttaattccgg attgcaaatt gaaagcgaaa ttgactttaa
    14821 tgacattaat ccccaaacac tctcctttct ccataaaatg gctcctttcg gacctgggaa
    14881 tatgcgaccg gtattctgca ctcgtaacgt aaccgtctcc ggaatgccgc gcataattag
    14941 tgaaaatcac ttaaaattca aagcggctca aaatcgttcg gttttaagtg ctatcggctg
    15001 gaaaatgggt tgctattacg aaatgttaat cagcaatcgc ccccttgata tcgcctttgt
    15061 aattgatgaa aatgaatgga atggctataa agaaattcaa cttaatatca aagatatcgt
    15121 atattctgac taccattaaa aaatgacagg agaagctgta ccaatgtaca aaagtaaaat
    15181 cgccagcctc gggaaaaatg ttcccgaaaa tattgtaact aatgctgacc ttgaaaaact
    15241 gatggatacc agtgacgaat ggatcgtcga acgaaccggt attcgcgaac gacgttttgt
    15301 aaccccaccc tgcggcgcca gccagctggc tctaccggcg gtactggaag cgattaccaa
    15361 tgccggatta actaaaaatg acattgattt aattatcgtt gcgacaacta ctccggaaca
    15421 tctgactccg ggtaccagca gttatttaca ggctttactt gaattaccca atattccgac
    15481 actcgacatt cgggtgcagt gcagtggttt tatatatgga cttgcgattg ccgacgcttt
    15541 tatcaaaacc ggacaatata aaaatatcct cctcgtcgga gttgaagtcc agtccgtcgg
    15601 gctaaatctg accaccgtcg ggcgtgatat cgccgtcctc tttggtgatg gagccggcgc
    15661 ggctgttatt acgcgttcca ctgatgaaag tgcaattctc tctactcatt tattcggcga
    15721 tggccgctat gcgaatgaac tctgggtaga agctcccgac ggttcaaaaa gaccgcgtct
    15781 ttccaaagag atgctggacg aagggaaaca ctatcctaaa atgaacggcc gtcaagtttt
    15841 taaacatgcc gttactaaat ttcctgaagt aatcaacatc gccctcgaag ccaacaatct
    15901 gaataaaagt gatatcacct taattattcc tcatcaggct aatttgcgta ttaccgaagc
    15961 cgttgcccag cgcctggaaa ttccaatgag ccaagtctat agtaatatcc ataaatacgg
    16021 caatacaact gcggcttcga ttcctctggc aatgtacgac gcacttcaag aaggcagata
    16081 taaaaaaggt gattatctaa tcctagccgc attcggttct ggatttacct gggcttccgc
    16141 cgctctacgc tggtagtaaa tattttaaat tatgagcata aatcgtggcg aattctaatt
    16201 catctaaaat agccggcaaa gagcgtggcg ttttccaatc caggcgccag cgcaccggtg
    16261 tatttttaat ctgcggagta atcgtcgtag ccttagcttt actctttccc aaaactcatt
    16321 acaacatcac taagtataaa ataaatgaca tcgcacgctc atcaataatt gcgccgttcg
    16381 acttcccaat cttgaaaaca gaacaggaat tagaagccga tcgtttagaa gccatcaaaa
    16441 aagttccgtt tgtttttatt caggataaag acgtcgaaaa aaccgaacta atgaacctcc
    16501 agaaattttt tacggaaatt gatcgcttac gattagcccg aatcaagtat gatgaatctc
    16561 tgcgaaatat tagtattaat cggagcgaag aatcgctggc aattttgacg gccgattcaa
    16621 ttactttttt tgctttacag aaaaaatttc tcaacgatta taagcttaac aaaaattcac
    16681 cagttctgaa gtccctggta tatcagtacg atcccaagaa cgtcaaccag ctctcggctc
    16741 atgtccagaa tcaggtaaaa tcggtgctta gcgagcttta tgttttacag attctcgata
    16801 ttcccaaatc ccagataatt tcggaaaaaa ttgccatcct tcagggcggc gaagaggtct
    16861 tagaaaatcc aaacgaggtt ttagaattat ccgaagcctg gacaaaagcc aaactggctt
    16921 tgcaagcccg cctaaatgac tacaccccag aagtggttaa tgccgcttac gacatcgtgg
    16981 tcgctttcct gaaacctaac ctcatttacc agaaacaaat taccgagaaa cggcaagcgg
    17041 aagccattgc gaaagtcccc atcagcaagg gagttgttct taaaaacgaa aaaatcgttg
    17101 acgctaacac gcgcattacg ccggaaatct atcagaaaat tgaatcaatg gctaaagaac
    17161 aagcgcgctt agccaatgtg caacgcagtt ggcggcgagc tttaccggta atcggcgatc
    17221 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcccaaatc ggtggcaatt
    17341 tcaactttat aatcgtgtcg gttttcgtga gcacttttgc gatttatgcg gtccggcaat
    17401 tgcggaagcg tagccaaatt ttcaaatcta tcatctatat cgtactcggg tattgcctgg
    17461 ctattttcgc caccgaaatg ttgaaatact cgcccccgaa atctatccta aataatattt
    17521 tctacgccac gattaatggc gttttcgcac cgttcctagc gtatggaatt atcgggctat
    17581 tggaaaacct ttttggaata accactgatt tatcactact cgaactggcg gattttaatc
    17641 aaccgctcct aaaattactt tcgcacgagg caactggaac ttttactcat agtgtaacgg
    17701 tcggcaatct ggctgaagcc gccgctgatg ctattggagc aaatgcccta ttggctcgcg
    17761 tcggttccta ctaccatgac attggcaagt tgactaaacc cgaatatttt atcgaaaacc
    17821 aggcttttga ttataaccgg cacgataaac tggcgcccaa tctcagcgcg attatcatcc
    17881 ttaaccatgt caaggagggt ctgcggctgg caaacgaata taaattaccc caggttattc
    17941 aggacttcat ttctactcac catggtacta ctcgcgttga atatttttac gataaagccc
    18001 tcaaaaaatc cgccaagccc gacgaaatca atgatgccga ttttcgctat cccggcccaa
    18061 aaccacatac taaagaaacc ggcatcgtta tgatttgtga aacaatcgaa gcggtatgtc
    18121 gctccctcaa caagccaact atgtcggcta tcgaaaaagt cgtggactcg gtgattgaaa
    18181 gccgactcaa agaaggtcaa ctcgatgaat gcccgctgac tttagccgat ctaaagaaaa
    18241 tcaaaggcga tctcaaggaa ggtaccggaa tactgccaat tttacggggc attcatcacc
    18301 tgcgtgtgga atatcccgga caggaaaaat tctccaattc ttccgtccca agtaacaaaa
    18361 agggaaaaaa tgacgatagt caaaatcttt aacaaccact ctcattacac ccttttaaaa
    18421 ccggcaatcg aacaggttgt ctcttacatt gtcaatatgg agcaacaagc cgttcagagc
    18481 atcaccttca tagctagcga tgataaaact cttaatgaaa tgaaagtcaa attattcggg
    18541 atcgatcaac tgaccgatac aatcagcatc aattacaatg agatagacca accggtagaa
    18601 ggcgaaattt atctcagtct cgatcgcatt gaggacaacg cccgcacatt taaagtcacc
    18661 tttgaaaaag agctcgccct ggtaattatt cacagcgttt tacatttact cggttataat
    18721 gatgacaacc taaacgacaa aaaacaaatg caagctcttc agaattttta tatccagcaa
    18781 attaaattcc ctcgccttta ccgtcagcgc cgtatccagc ctaattatga atgatgaact
    18841 ttcccgctat tttgatgaac taatcgagat cgtgaaaacc ttgcgcggtc ccaatggttg
    18901 tgaatgggac aaagcccaga ctcgtgattc tcttacgcct tatttgctcg aagaagccca
    18961 cgagctcatt gactccattt ctgaaaataa cccctcgaaa ataaaagaag aactcggcga
    19021 tgtactgctc catataattt ttcaggctca gatcgccgcc gaagccggtg aattcaccat
    19081 tgccgacgct ttgcaacaaa tcaacgccaa gttaattcga cgtcatccgc acgttttcgg
    19141 cgaaactaaa gttgaaaatg tcaacgacat caagaaaaat tgggaagaaa ttaagctgaa
    19201 agaggggcgc caatccctga tggacgggtt gcccaaatct ctaccggctt tactacaagc
    19261 ccgccgcgtc caggaaaggg cggctcaagt gggtttcgac tggtctacca tcgaaccagt
    19321 ctgggctaaa atcaccgagg aaacacgtga attgcaagaa gccattgcta ctcgtaagaa
    19381 agatgagatc gtcgcagaat tcggcgattt actttttaca atggtaaatc tttcccgctt
    19441 ccttgatatt gatccggaag aagccttgcg acaaacagtc actaaattta tccggcgttt
    19501 tcaggctatc gaaaatgaat tagccgccac cggtaaaaat ttcaaagata cctcgctgga
    19561 agagctggat aaaatctgga ataaatcgaa agataaagaa tcctgaatat taaggccact
    19621 aactctcggt ttagtaaaaa atcattccca caatataatg aaatttttcg ccgaacctat
    19681 tgacgaatat cttaattaat gctatattgt ttacgtatga aacgcttatt gaaaaattat
    19741 cctgcaatag tgtggcgagt gtttttggaa ccaacaccat aaacttggga gccggactcc
    19801 gaacggctaa agcacgcttc ctcgttgaag aaacttgatc cgttcgggag gtacccaccg
    19861 tgattttcac ggttccctta atccgccagc gctgttgcag gatattttta tgtagtgata
    19921 attaaaccag gaatcgagtt caattccgtt cggaactcat ccgctttctg aacttcaatt
    19981 tgcaccgtaa ctatgacgtt attttcggta aattccggct tgctcgattc cgaaaaacgc
    20041 ccgagaaaat gtgccagctg tgagtagttc gctagcgggc attcgattct tagggtttgg
    20101 cgctggataa aaggtttgtg ttcgacaaga tcgagcgtct tcccggccgt agcgccgtat
    20161 gctctaatca agcctcctat tcccagcttc gtcccgccga aatatcgcac gactacgaca
    20221 agcgctttga ccaactgccg gctggttagc gcttttaaga tcggcgctcc ggctgaaccg
    20281 cgtggctcgc ccgcatcgtg agcataatat agcaattgat tattttcata aattcgatac
    20341 gcccagcaaa tgtgagtagc ttttcggtgc ttctggctaa tttccgctat tttttggact
    20401 gcttcggact tatcggcaac cggaaaacaa tacccgataa actttgaatc tttgactgta
    20461 agcataattt cagccgaatc agcaattgtt tttagctctt gaggtaacat ttcagccata
    20521 aattttatat ttacttcagt agtaaaattt tgcgcgttag attcttgctt ccgatcgcaa
    20581 tttgcataat atagacaccg gatgcaagtt gcgcctgtct taattcatca ctcgaaaatg
    20641 ataatgaata attaccaatc tcgatcagtc cattatcaaa attcttctga aatacatttc
    20701 gtcccgtaag gtcaaaccag ttcgtcttga ttgaattaac aatctgaccc acctgtagat
    20761 ccactatcaa atctcccgat tttcccggag aatacggatt tggatagacg tttttaatag
    20821 ttgacgctat tcgaaattca tcacccacct cggaaattgt cacagtcaga ttaggagttg
    20881 tagttttact gctattagtc ataaaaataa gtagagaatc attattggcg gcattaccga
    20941 ctaataaatt ttgattaagt gacaatgtct cggctatttt tccgacccga tttttaatga
    21001 acaagtaacg tccgagaaaa ttctccggcg acgatgaatt gacatttatt ccacataacc
    21061 aattctggtc gaacactact tgatatggca cgcacgcaaa gggttctatt gatggagtaa
    21121 aaaccgcact ttctgtaaag acaactttgt tagttgccgg aatttgtaac aatggaaatt
    21181 gcatagcatc gctggaaagc gcccaatcgg gatcatagcg ttctccggta aaatagcatc
    21241 ggagataaaa ctcattccac aaatcggcta aatcggcatg ataatcatcc gccagaaccg
    21301 cctgaagcga ttcgaaagca taattagtct tgatctcctc ccagactttg accaagagat
    21361 tctcgccata acgatctaac ataaatctaa gaaaagctcc cagcgcatag gcataatcat
    21421 aattccaaag cgatttaagt ggagaattga aaaaagcgcc gacataaaag agataatcgt
    21481 tcacttccgg ataagcccgt tcttcgaacc aggtcgagca ccattccagg aaataatcat
    21541 cggcgcttgt ccgcccgcca cccgaatttg ccaagccgtt accttcccac caattgtaac
    21601 cgagctgtac cacgtgaaaa aattcatggg caactgtgac tcgcagacca tttaagcccg
    21661 ttgtcggata tccaacaaag tcattatcaa ttaccgtata ggcggtatag tcataggggc
    21721 gatcgggaat agtctgaacc ggattttcat cataggtata agcataggcg gccccaccca
    21781 gattaataat gtaaatatcg gtttctggac taaatatatc atcaaacggc ggtgattgaa
    21841 aaccaagtgt atcaattagc aaactataag accgctcggc ggcnnnnnnn nnnnnnnnnn
    21901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21961 nnnnnnnnnn nnnnnnnnnn nnnacaatca gacatacgat tgtaaaagtt aattgacgga
    22021 ctattttcat ttattccgga aggaaattat cagcggcact ccggaaaaac cgaaccggcg
    22081 gcgcagttga ttttctaaaa agcgtcggta ttcttctttg attaagcgcg gctcattaca
    22141 gaaaaaaaca aaaaccggcg gctcggtttt gacctgggtg atgtatttta tcttgataaa
    22201 tttgcccaga tttgccggag gcggtgtgcg gtcaatgaca tcctgcagga attggttcaa
    22261 ttcacttgtt ccaatgcgta ttttgcgatt ctcatagacc atccgagcag tttctaaaat
    22321 tttatgcacg cgctggtgag ttttagctga aataaaaatt atcggataat gttccagttc
    22381 ccgaaattgt tcgatcaaat tacgtttgaa attaacctgg gtattagttt cgcgctgaac
    22441 caaatcccat ttattaatcg caattaccaa gccttttctc aagtcaatga cctggcggac
    22501 gatggccgca tcctgacggg taaatccttt aactgcgtca acaactacca gcgccacatt
    22561 acagcgctcg attgcccgat aagcacgcag aagactataa tactccactt catctttaat
    22621 ttggcggtgc ttgcgcattc cggcggtatc tatcaaagta accggttcat taaaatattt
    22681 tatttttgta tcaatcgcat cgcgggtcgt gccgggaacg tcggttacga tagccttttc
    22741 cgagccggct aagacattta aaatcgaaga tttgccgaca ttcggcatac cgacaattgc
    22801 taattgcaaa tggtctttct catcatcatt agcggcgatt tttcgtggaa ttctctgcag
    22861 aatggcatcc agtagatcgc cgatatggcg tccattcaaa gccgaaatcg gaaaaacatc
    22921 gcccaagcct aattcggtaa attccagact attaacttcc agtcgctcat tgtcacattt
    22981 attggcaacg attagacaat cccgctggga gcgtcgaatc atctcggcaa tttcacgatc
    23041 gagggctgtc atcggttcac tggcatctac tacaaaaata accagattgg cttcttcaat
    23101 ggctaatcta acctgttcac gaattgcttt cgttatttga ttggtgcttt ctgggataaa
    23161 gccgccggtg tcaatcagca aaaattgtcg tcccgcccaa tttacagttt cgtaaacccg
    23221 atctcgcgta ataccggctt ccgaatgcac gatagattta cgcttctggg ttaggcgatt
    23281 gaataaagtt gatttgccga cgttcggtcg tccaacaatt gctacaatcg gttcagccat
    23341 tatcttcgtc cactaattta ccgaaatcgc catcccagac ttgcaccggt ttgcgcaaag
    23401 cctcggcaat ttgataagga tataaatcat cgagtaaaat tccatcttca ttaacaattc
    23461 gcggtggcaa aacaattaaa tcatactcat caacattgat tgcctgagca agaatatctt
    23521 gaccggtcaa taatcctgcc accgttaccg atggcccaaa gaaattatta attactggta
    23581 aaatatccac cgagaagtta ttgatctgat tcaatcgcgc cgtgacatat ctacttaaaa
    23641 ccggcgccgc cagcatacct gtaacgaaaa gcactctttt cctcttacta atagcgtgtg
    23701 gtaatttacg actttgccgt ttgaaatcgt caagaaatgc ccgcgtcgtt ccgaccccat
    23761 tttcaatttg gtaaaaactc ccataaaatt tgcatcccgg taaatcttga ccgactaaca
    23821 aataaaattc atcggagaga aaaacccaat gctctccatc gcgatttacg tagcagcgat
    23881 tccaagttcg aatattttca agcaattgag atgccagatg cgaatccacc gctggaataa
    23941 cgggaaggtg ttggcgatgc gcagtcagac cgactggtac gattgccacc gataatatcg
    24001 aatcccggaa aatatataaa tccgccagcg ttttttcgag tactgccccg tcgttaacac
    24061 ccggaattag tactatttga gtatgtagat caatccggtt ttgggctaga aaagtgagct
    24121 tttgcatcaa attatccggc tttttcaatt gaaaaatatg gcgtcgcaca ttttcatcgg
    24181 tcgcatgaat cgaaacgtac aggggcgaaa ggcgttgcaa gacaattcga ttaagatcgg
    24241 cttcacttaa attggttagg gtgatataat tcccgagtag aaacgaatag cggtaatcct
    24301 catcgcagaa ataaattgcc ggtcgcattc cgggcggatt ttgcttgata aagcagaaaa
    24361 tgcagttatt tccacaggct ttcaggcgcg gcggagataa ttcaatcccc agtggttcat
    24421 cggcattttt agcgatctgg aatgttttct tatgaccatc ccgaaggaca acaactttac
    24481 aattttcatc agattgatga aaatacaaat cgaggaaatc gttgatcggc tggcgattga
    24541 tgctgacaat ctggtcgccc cgacggatac cgttcatttc agccaggctg ttgggcttta
    24601 ttccgacaat ttcaaccacg attccagcac catattttat aattaattaa aaattgcttc
    24661 atattaataa aaactgtcca aaattcgcta atcccgaccg gaaaaacaaa attaat
//
LOCUS       DAOMJU010000026        24253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1922_length_24253_cov_21.267873, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000026 DAOMJU010000000
VERSION     DAOMJU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 24253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24253
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1922_length_24253_cov_21.267873"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..190)
                     /locus_tag="PLO58_03980"
     tRNA            complement(120..190)
                     /locus_tag="PLO58_03980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Gly,seq:ccc)
     gene            479..2893
                     /locus_tag="PLO58_03985"
     CDS             479..2893
                     /locus_tag="PLO58_03985"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HQO73982.1"
                     /translation="MSESITIDFKNPNNESPEKNIPENSLSINPENIQVTPEKKPLFS
                     KNAMEVLTKRYLQHDKNGNAVETVEELFRRVAEAVAEAEANYSPRSSIPRTTEKFYQM
                     MIRKDFLPNSPTLMNAGRELGQLSACFVLPIDDSMESIFQTLKDTALIHKSGGGTGFS
                     FSRIRPKKSIVKTTSGVASGPVSFMKVYNAATEAVKQGGTRRGANMGILRVDHPDILE
                     FVDCKKNSNELTNFNISVAVTDEFMEKVRRREQYELVNPVDNTIVGTLNAAEVFKLIA
                     QNAWYNGDPGIIFIDRINQMNPTPKLGDIEATNPCGEQPLLPNESCNLGSINLANFIS
                     ENKIDYKRLGETIDLAVQFLDDVIDINKFPLKKIETMVKKTRKIGLGVMGFADVLFSL
                     KIPYDSDEALQLADELMKFIYNQTKEASTKLGESRGTFPAWNDSIYFPDGPKYRNSTC
                     ITIAPTGTISMIADCSSGIEPIFSLAFTKKVMDGTELLYVNPYFEQACKEAGIYSAEL
                     MHRVIAEGSIQHIAEIPEKIRRVFVTAHDISPEWHVRMQAAFQKWVDAAVSKTCNFPK
                     SATVQDVLDAYWLAYEAGCKGITIYRDGSRDEQVLYVGKQTDQAKSEQAVQPPIIDQT
                     LHPRIRPPETHGVTKRVRTGNGTLYITINEDDYGLCEVFTNIGKAGSQAQVESEAISR
                     LISLALRSGIDARDVVKQLKGIGGPSPIWENGTQILSTPDAIAKALEWYLDEREGKKK
                     TTGNQPSFSLLDVESTTTVKKPEPTSQHTAPHNKKSITTCPECGSTVYHESGCVTCPN
                     CGFSKC"
     gene            complement(3174..4253)
                     /locus_tag="PLO58_03990"
     CDS             complement(3174..4253)
                     /locus_tag="PLO58_03990"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HQO73983.1"
                     /translation="MALLEVCLVNDLPIIGYLSAILKKPDAIVWLRTPNQKKVVSLIE
                     TALSGQISSTFESREINPNDVPEFIEQCQKVLRDYPEHDVILNISGGSRLHAVLAAEV
                     FKNAGKEVILIDPDNSRIVDVKTGEVKTFQFNLTVNEYIALHGIKMESGTRFDPEIGK
                     RSALSFFIGNNLDRIMPFIDSHRDEWNNMGENKKTVHWKIDDQRLRFVVNYDAEQNVM
                     RFRFGTGENQKTVDIAGDGGNYLFNGGWLRELVFLRVHRQGDDVRLNVRIDRESVPEA
                     MRCESMIDICMMKGCNFYIFQCFSYPITRESFIELQAIHETVRILKAKGFIFMSHRPH
                     RGFIERASDIGLEVITGHRIANFSL"
     gene            complement(4418..5536)
                     /locus_tag="PLO58_03995"
     CDS             complement(4418..5536)
                     /locus_tag="PLO58_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HQO73984.1"
                     /translation="MEKWRKLVQKSYTTVDQITKALNLPNDELAGLDKVFRIKVNPYY
                     FSLIKKKGDPIYRQVIPDKAELEDCGLEYDPLSEEKDSPVPSIVHRYPDRCLFLVSPE
                     CASYCRFCTRKRKVGDAQKISPRFIQQGIKYIAEHPEIRDVIISGGDPLMLSDQKIER
                     ILSSLRQIPHIQILRIGTRVPCFLPQRITTKLVRILKKYHPLYINVHFNHPDELTPEA
                     VKALGRLADAGIPLGNQTVLLKGVNDDPEVMKLLMQKLLQARVRPYYIYQADMVYGTE
                     HFRTRVEKGLEIIKALRGWTSGLAVPHFVIDAPGGGGKIPLLPEYVKSISDEEVVMCN
                     YEGKEFHYRQAGKPPAPPTCVRDIMAEALVAENDLALN"
     gene            complement(5540..6028)
                     /locus_tag="PLO58_04000"
     CDS             complement(5540..6028)
                     /locus_tag="PLO58_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQO73985.1"
                     /translation="MNMIREIKSTDRAEILKILSDTQMFTTAEIRVAMELIDTALTKP
                     NQTDYLIFVAINGQKLAGYVCYGPTPATEGTFDLYWIAVAPQVQGKGIGTDLLTYVET
                     AVQAQHGNLIIIETSSQEKYLPTRNFYLKNGYAIAAQIKDFYRRGDDRVIFVKYFKQE
                     NK"
     gene            complement(6025..7041)
                     /locus_tag="PLO58_04005"
     CDS             complement(6025..7041)
                     /locus_tag="PLO58_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO73986.1"
                     /translation="MSSESQKIKILVVFNDLQSVDSNFDPDIISEIAVRDEAAAVYTA
                     LNNCGYVAEMLPILNIDEDLIKINSFQPDVIFNLCEGWQSRTDQEMNVAGLWEMLDLP
                     YTGNSPLTLGLAQNKVLSKNIFKAIGIPTPDFYVYESIPETCSLDFPLIAKPSREDGS
                     IGITQNSVVNDLIELKSIVGELLAKYKQPILVEKYIAGREFNVSILGNENPLVLPPSE
                     INFEQVSEEYFPITSYEAKWLSDHPVYQMTPPVCPAPISAELNEKLTSLSLAVYHALR
                     GRDYGRIDIRMDAAGNLFVLEYNPNPDISPSAGFVRALNAAGISYETFVVFLIKQALN
                     RRRR"
     gene            complement(7041..8093)
                     /locus_tag="PLO58_04010"
     CDS             complement(7041..8093)
                     /locus_tag="PLO58_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQO73987.1"
                     /translation="MKIALTYSTKEGLRKEYRKRFAVDPDDEDIPPDLFAEGDDPQTI
                     EAIVDALQLNNHQVVSFEGDDEVAHGLEQSRPDIVFNVTEGLFGDWRESYVPLICERL
                     GLPYTGSDPLTLAICLNKARCKEILSYYHIPNATFQVIYPGDDGFAENFKFPAIVKPL
                     AEGSSKGIFDNSVVYSKEQADDVIASCFAKYNNEPVIMEKYLAGPEFTVAILGNSPEM
                     EVLPIIGMDFSQLPTGAQPIYSYEAKWIWDRPEKPLNIFTCPAQIDDNLKNQIELLVR
                     RTCRILNIRDWCRLDVRLDENGVAHILEINPLPGILPNPEDNSCFPKAARTAGYTYPA
                     MLNQVVQVAARRYGLS"
     gene            complement(8243..9418)
                     /locus_tag="PLO58_04015"
     CDS             complement(8243..9418)
                     /locus_tag="PLO58_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HQO73988.1"
                     /translation="MKNQLSLKIRAVAMTALLIYSLTVTARAQTNLGLWVIRDQLNSK
                     EKIDAFVNFAVLNDIKDLFVQVRGRGDAFYHSEIVVYSELLSNNNWDPLEYTLSQAHS
                     YGLRVHAWVNVFLLWSSDQKPKNENHLLYQHPEWCTVDANGIKDIQRRATDFNTLGTE
                     GIYLSPLLPEVRKYLIDIIQELATNYDLDGIHLDYVRYAKNCYDYNPIGRILFREKYG
                     IDPLLLTLSDKSLYKGLELAEIDSLMLKWEDFRRDAISDFVHNARKNLREIRPSLQLS
                     AAVKPDPDDACKNFFQDWQYWLRQDWLDFVVLMNYTKDTKQFQKILRKIDPNINKNNV
                     WVGISVYNQSQYEAMTKTILTLNNQFQNIVFFSYETFLKDPQYYPVVRKAFEIAHPR"
     gene            complement(9439..10032)
                     /gene="rplQ"
                     /locus_tag="PLO58_04020"
     CDS             complement(9439..10032)
                     /gene="rplQ"
                     /locus_tag="PLO58_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQO73989.1"
                     /translation="MRHQKKVAKLGRSTDQRKALIQSLTKAMLINGRIKTTDAKAKEL
                     RRVVERLITYGKRDTVHSRRLAFRFIQDRDLVNKLFEDIAPQYANRNGGYTRIIKLGY
                     RDNDNAAVSLIEFVDYKQPEEKKKKAGSEATKSVQGKKEEKSTEVEKEQTPAKTKPTK
                     KTQAKKEEKQSAQAEVKEDIPPENISGEKAEPASETE"
     gene            complement(10054..11034)
                     /locus_tag="PLO58_04025"
     CDS             complement(10054..11034)
                     /locus_tag="PLO58_04025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQO73990.1"
                     /translation="MTVYDQIIARVVKDENSNDTHGIYYLQPLERGFGITIGNALRRV
                     LLTSIPGAAICSIKIDGVLHEFSTIPGVREDVAEIILNLKQVRLKLFDPKPDKVTLHF
                     KGAGEFTAGMIGENITQFKILNPDLHICTMNEDADFNIEIRIARGTGWVPAEKNKLPD
                     YPIGTIFIDSIFSPILNVSYKVENVPGTAREILEKLTLEITTDDSITPDESLDLASKI
                     LRDFFSLFSRVEAKPIEFQREEPSEDVLKIRNLLKKSVDEMELSVRSYNCLKANNIRT
                     IADLVSHEEADMLKFKNFGRKSLNELMTKLKSMGLEFGMDVDKYLVEKEE"
     gene            complement(11046..11678)
                     /gene="rpsD"
                     /locus_tag="PLO58_04030"
     CDS             complement(11046..11678)
                     /gene="rpsD"
                     /locus_tag="PLO58_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQO73991.1"
                     /translation="MARYTGPVCKLCRREGKKLFLKGERCNSTKCSFETKSYIPGQHG
                     SQTAHPRLSNYGIQLREKQKIKRIYGVLEKQFRQYFELANRQKGVTGTNLMKILERRL
                     DNVVYRLGFASSRRMARELVSHRHFTVNNRVVDVPSYQLKAGDVIQVREKSKKLQVIH
                     DSMKRIRGEHELPWLILDKAKMQGIFIQVPERDQMNVDVNEQLVVELYSK"
     gene            complement(11701..12093)
                     /gene="rpsK"
                     /locus_tag="PLO58_04035"
     CDS             complement(11701..12093)
                     /gene="rpsK"
                     /locus_tag="PLO58_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQO73992.1"
                     /translation="MPPKRSTVKKKKRTKVESEGIATIKATFNNTIISLTDNYGNVIS
                     WASAGTAGFKGSRKNTPYASQQAATKAAQEAINLGLQRVEVRVKGPGAGREAAIRALN
                     LAGLQISAIKDVTPIPHNGCRPPKRRRV"
     gene            complement(12111..12497)
                     /gene="rpsM"
                     /locus_tag="PLO58_04040"
     CDS             complement(12111..12497)
                     /gene="rpsM"
                     /locus_tag="PLO58_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQO73993.1"
                     /translation="MARIAGVDLPNEKKVKIALAYIYGIGRANAMDILQKAGVDPEIR
                     VKALTEEQVRDIRNVISNEYKVEGTLRTEVQMNIKRLMDIGCYRGLRHRRGLPVRGQR
                     TKTNARTRKGPRRTVSRKKVKVGKKG"
     gene            complement(12517..12630)
                     /gene="rpmJ"
                     /locus_tag="PLO58_04045"
     CDS             complement(12517..12630)
                     /gene="rpmJ"
                     /locus_tag="PLO58_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQO73994.1"
                     /translation="MKVRSSVKKICNKCKIIRRKGVVRVICTNPKHKQRQG"
     gene            complement(12647..12865)
                     /gene="infA"
                     /locus_tag="PLO58_04050"
     CDS             complement(12647..12865)
                     /gene="infA"
                     /locus_tag="PLO58_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQO73995.1"
                     /translation="MVKEKLIKVDGIIKENLPNASFRVVLENGHEVLAHVSGKMRMHF
                     IKILPGDKVTMELSPYDLTKGRITYRYK"
     gene            complement(12878..13627)
                     /gene="map"
                     /locus_tag="PLO58_04055"
     CDS             complement(12878..13627)
                     /gene="map"
                     /locus_tag="PLO58_04055"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007553999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQO73996.1"
                     /translation="MVHIRTAEEIEKIRESCRIVSETLDLLSREIKSGITTLYLDHLA
                     AEFIKSQGGHSAFKGYNGFPGNICTSIDEQVVHGIPSNRVLKEGEIISIDVGVKKNGY
                     YGDAAMTFAVGTVSDDRLQLMRVTEDSLYQGIAQARPGNHLSDIGHAIQTHVESYGYG
                     VVRELVGHGVGRELHEPPEIPNYGEPGRGPVLKPGMCLAIEPMITMGSYEVRLLKDGW
                     TVITEDHLPSAHYEHTIAIGPDGPQILSQRK"
     gene            complement(13637..14977)
                     /gene="secY"
                     /locus_tag="PLO58_04060"
     CDS             complement(13637..14977)
                     /gene="secY"
                     /locus_tag="PLO58_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQO73997.1"
                     /translation="MIQQFQSIFKIPELRKRIGYTLALLIVFRIGAHIPIPGIDTEVL
                     GAAMSSFANTLFGLYDMFSGGAFAQATLFALGIMPYITASIILQLLGAVIPYFQKLQK
                     EGEEGRKKLTQMSRYGAVIIAALQSFGVAQFLMSPQMSARVGNQMLPVVPRPGVGFVL
                     MTMLTITTGTVLLMWLGERMTERGIGNGTSMIIMVGILARLPNVFVSEITLIREGVRG
                     LFTEIILIVLMIVIVGFVVLLTQGTRRIPVQYAKRVVGRKVYGGQATHIPLRVNTAGV
                     MPIIFAQSIVFIPSTIATFFPNSNFVQGMMRIFSVQSIVYWLIYGVLIVFFTYFYTAI
                     AFNPVEVADNLKKYGGFIPGIRPGKKTAEFIDNILTKVTLPGSIFLAFIAVFPYILMK
                     IMDINYDLASFFGGTSLLIVVGVALDTLQQIESHLLMRHYDGFLKSGKLKGRRG"
     gene            complement(14974..15411)
                     /gene="rplO"
                     /locus_tag="PLO58_04065"
     CDS             complement(14974..15411)
                     /gene="rplO"
                     /locus_tag="PLO58_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQO73998.1"
                     /translation="MGLNTLKPAYGSVKNKKRLGRGPGSGHGGTAGRGNKGTGARGSN
                     KKPVYVEGGQMPIYRRLPKRGFKNLFKEEFQVVNLDHIARLQITEVNPEILQQKGLIK
                     SLKKPVKILGRGELSGAITVVAHAFSTRAKEKITASGGKVELL"
     gene            complement(15415..15618)
                     /gene="rpmD"
                     /locus_tag="PLO58_04070"
     CDS             complement(15415..15618)
                     /gene="rpmD"
                     /locus_tag="PLO58_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQO73999.1"
                     /translation="MTKKDKTAMLKITQVKSGIGYCEKTKRTLLALGITKMNQTVLLP
                     DNPAIRGMINSIPHLLKVEEIKE"
     gene            complement(15615..16124)
                     /gene="rpsE"
                     /locus_tag="PLO58_04075"
     CDS             complement(15615..16124)
                     /gene="rpsE"
                     /locus_tag="PLO58_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQO74000.1"
                     /translation="MRKSIDPTQLELLEEKVIKINRVAKVVTGGRRFRFNALVAIGDG
                     KGHVGIGLGKALEVSSAVAKGKENAKKNLVKIPVINGTIPHEVQAKYGAAKVILRPAT
                     PGTGVIAGGAIRAIMESLGVRDVLSKSLGSNNSHNVVKATMLALSKLKDPYMVAHRRE
                     KTLQEIFGS"
     gene            complement(16143..16517)
                     /gene="rplR"
                     /locus_tag="PLO58_04080"
     CDS             complement(16143..16517)
                     /gene="rplR"
                     /locus_tag="PLO58_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQO74001.1"
                     /translation="MNKVKNIKAFARQRRQKRNRHKIQGTSERPRLTVFRSLNHIYAQ
                     VVDDNLHKSLLAVSDLTPEVREQIKSNTKKSDRSALVGKTLAEKAVAKGITKVVFDRS
                     GYQYHGRVKALAEAARQAGLEF"
     gene            complement(16529..17071)
                     /gene="rplF"
                     /locus_tag="PLO58_04085"
     CDS             complement(16529..17071)
                     /gene="rplF"
                     /locus_tag="PLO58_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQO74002.1"
                     /translation="MSRIGKKPIEIPKEVNVTIQDCSITVKGPKGELKWDFPSDMKVQ
                     INDSQIIVTRPSDSRNHKALHGLTRVMIANMIQGVLQGFSKTLEIEGVGYSAEMKGKS
                     VQINVGYSHAILVTPPEGVSFEVPKALTIVVKGIDKQKVGQIAAKIRSIKPCEPYKGK
                     GIRYQNEHIRRKAGKKVGVK"
     gene            complement(17095..17493)
                     /gene="rpsH"
                     /locus_tag="PLO58_04090"
     CDS             complement(17095..17493)
                     /gene="rpsH"
                     /locus_tag="PLO58_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQO74003.1"
                     /translation="MAVTDPIADYLTRIRNAQAANHRWVDIPASNLKKRISLILKHEN
                     FIKDFILIKDGKQGLIRIYLRYSKTGEPMIEGLQRISKPGRRVYAASDNIPRVVGGMG
                     IAILSTPMGVLTDKVARKNNVGGEVLCYVW"
     gene            complement(17510..17695)
                     /locus_tag="PLO58_04095"
     CDS             complement(17510..17695)
                     /locus_tag="PLO58_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HQO74004.1"
                     /translation="MAKKSLIAKARRNPKFSTRRVNRCSICGRPRGYYRKFGLCRLCF
                     RELALRGEIPGITKASW"
     gene            complement(17697..18281)
                     /gene="rplE"
                     /locus_tag="PLO58_04100"
     CDS             complement(17697..18281)
                     /gene="rplE"
                     /locus_tag="PLO58_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQO74005.1"
                     /translation="MTAYETEIVPAMVKKFGYKNIMQVPKLVKISINMGVGKTKEDPA
                     SLKKAVEDIRIITGQQPMVTKAKRAISNFKIKTGDPVGCAVTLRRFRMYEFMDRLIST
                     ALPRVRDFSGLSDKSFDGRGNYSIGLKEQIIFTEIDYDKVDHIRGMNITFTTTAKTDT
                     EAYELLSLLGLPFKKRAIKESTTGGEVKTAMEIN"
     gene            complement(18328..18642)
                     /gene="rplX"
                     /locus_tag="PLO58_04105"
     CDS             complement(18328..18642)
                     /gene="rplX"
                     /locus_tag="PLO58_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQO74006.1"
                     /translation="MLHIRKNDTVMVITGESKGKIGKVLKVFPDKERVIVEGVNFIKR
                     HTRPNQQNPQGGIIEKEAPIHISNVKLVHADQATKVGYRTLKDGKKVRVARETGEIID
                     AI"
     gene            complement(18655..19023)
                     /gene="rplN"
                     /locus_tag="PLO58_04110"
     CDS             complement(18655..19023)
                     /gene="rplN"
                     /locus_tag="PLO58_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQO74007.1"
                     /translation="MIQQESRLKVADNTGAKEVMVIRVMGGSHRKYGTVGDTIVVTIK
                     QATPGGMVKKGEKSKAVVVRTTKEVRRKDGSYIRFDDNAAVLIDNNLEPRGTRIFGPV
                     ARELREKNYMKIVSMAPEVL"
     gene            complement(19047..19298)
                     /gene="rpsQ"
                     /locus_tag="PLO58_04115"
     CDS             complement(19047..19298)
                     /gene="rpsQ"
                     /locus_tag="PLO58_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQO74008.1"
                     /translation="MISSDRRKILIGTVISHKQDKTAVVSVERTVKHSTYGKYIRRTS
                     KFYVHDAENKCHEGDKVKIVESRPLSRLKRWRLLEIIER"
     gene            complement(19317..19535)
                     /gene="rpmC"
                     /locus_tag="PLO58_04120"
     CDS             complement(19317..19535)
                     /gene="rpmC"
                     /locus_tag="PLO58_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQO74009.1"
                     /translation="MKKSTLDELTVNELETKLRDNLTALENLRFQKALQQLENPLKIR
                     HVKREIAQIRTVLHEYELGIRSKDKPNK"
     gene            complement(19537..19956)
                     /gene="rplP"
                     /locus_tag="PLO58_04125"
     CDS             complement(19537..19956)
                     /gene="rplP"
                     /locus_tag="PLO58_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQO74010.1"
                     /translation="MLAPKKIKWRKQQRGRMTGIATRGNAVSFGHYGLKAMEPGWIDS
                     RQIEAVRVAISRQIRKHGKMWIRIFPDKPITKKPAETRMGKGKGTPEFWVAVVKPGRI
                     LFEIEVASEQEAKDIFKTATHKLPIKTKVVARRELGA"
     gene            complement(19974..>20322)
                     /gene="rpsC"
                     /locus_tag="PLO58_04130"
     CDS             complement(19974..>20322)
                     /gene="rpsC"
                     /locus_tag="PLO58_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQO74011.1"
                     /translation="TDVQINVNEIKRPELDASLVGQNIAAQIVKKVSYRRAVKKAIQS
                     TMRMGAEGIRVQCSGRLGGAEIARTETMREGRVPLHTLRADIDFAAVTAHTTYGCIGI
                     KVWICNGERFKKS"
     assembly_gap    20323..20422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20423..20761)
                     /gene="rpsC"
                     /locus_tag="PLO58_04135"
     CDS             complement(<20423..20761)
                     /gene="rpsC"
                     /locus_tag="PLO58_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQO74012.1"
                     /translation="MGQKTHPIGFRLGFNKEWSSYWFDERNFTEKLTEDIKLRRYVLK
                     RLPNAGISRVDIYRTPKKITITINTSRPGIVIGKKGEEVELLRLELQKVTKTDVQINV
                     NEIKRPELDAS"
     gene            complement(20773..21141)
                     /gene="rplV"
                     /locus_tag="PLO58_04140"
     CDS             complement(20773..21141)
                     /gene="rplV"
                     /locus_tag="PLO58_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQO74013.1"
                     /translation="MEAKAVHRYVRQSARKVRLVADLVRGKDVETALNLLHFNSKKAS
                     ENVEKVVRSAVSNLLNSEGGSKLNPEDLYLKTIYVDGGSIAKRFRAGSLGRASIIRRR
                     LCHITVVVAEKENKQKSKVQ"
     gene            complement(21150..21425)
                     /gene="rpsS"
                     /locus_tag="PLO58_04145"
     CDS             complement(21150..21425)
                     /gene="rpsS"
                     /locus_tag="PLO58_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQO74014.1"
                     /translation="MSRSLKKGPYIDVKLMKKIEQMNSSGKKRVIKTWARRTTISPEF
                     VGHTFAVHNGNKFIPVFVTENMVGHKLGEFAHTRIFRGHSGAEKKGA"
     gene            complement(21442..22269)
                     /gene="rplB"
                     /locus_tag="PLO58_04150"
     CDS             complement(21442..22269)
                     /gene="rplB"
                     /locus_tag="PLO58_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQO74015.1"
                     /translation="MGIKTIKPITPGQRFKTVDTFEDITKKRPEKSLLEPITQSAGRN
                     NYGRVTSRHRGGGQKRAYRNIDFKRNKYGIEATVKAIEYDPNRSARIALLFYKDGEKR
                     YIIAPDGLVTGSVVLSGEEAPIKVGNCLPLKNIPAGTFVHNIEMRPKKGGQIARGAGT
                     AAQIMAKEGKYVTLKMPSGEIRLINEECYATIGVVSNKDHENISIGKAGRSRWMGRRP
                     HVRGVAMNPIDHPMGGGEGKSSGGRHPCSPWGQLSKGYRTRKKRNPSNKFIIKRRTK"
     gene            complement(22274..22594)
                     /gene="rplW"
                     /locus_tag="PLO58_04155"
     CDS             complement(22274..22594)
                     /gene="rplW"
                     /locus_tag="PLO58_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQO74016.1"
                     /translation="MSSKSIIIKPILTEKINELQESQNKYGFKVHPSANKIEIKKAVE
                     DKFGVKVEKVATQNIMGKTKLMTVRSGGRVIRTTGRRSNWKKAIVTLKEGEKIDFYQG
                     ETAV"
     gene            complement(22597..23244)
                     /gene="rplD"
                     /locus_tag="PLO58_04160"
     CDS             complement(22597..23244)
                     /gene="rplD"
                     /locus_tag="PLO58_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQO74017.1"
                     /translation="MSIEFSVYKLDGSDSGQKLVIETPAFDIEPHDHAIYLAVKTEMT
                     NSRQGTHSTKTRGEVRGGGKKPWKQKGTGRARVGSRRSSVWVGGGRAFGPKPHPYKMK
                     INKKVKSLARQSALAYRYREGALKIIEELSFDEPKSRNIRELLKKFDLLDKKVTILTA
                     NLNTNLLLASRNFYNVLVLEAEKASTYDLLDCEVLLIDKSGFEKLNNALLGDSRN"
     gene            complement(23250..23870)
                     /gene="rplC"
                     /locus_tag="PLO58_04165"
     CDS             complement(23250..23870)
                     /gene="rplC"
                     /locus_tag="PLO58_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQO74018.1"
                     /translation="MMIGIIGKKIGMSQIFKENGELIPVTVIAAGPCKVVQVKTNEKD
                     GYASVQLGFMEAKERLMAKPNLGVFKKNNITPLKYLVEIRDYDPKFCQVGETVTVALF
                     NPGDKVKITGISKGKGFQGVVKRHNFGGGPKTHGQSDRLRAPGSIGASAYPSHVIKGV
                     RMAGRLGGKRCTVNNLTVVKIDPESNLLYIKGAVPGSKNSILTIRR"
     gene            complement(23879..24187)
                     /gene="rpsJ"
                     /locus_tag="PLO58_04170"
     CDS             complement(23879..24187)
                     /gene="rpsJ"
                     /locus_tag="PLO58_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQO74019.1"
                     /translation="MPGQRIRISLKAYDHNLLDKSTRKIVATAKSTGAMVSGPIPLPT
                     QRTVYTVLRSPHVDKKSREQFQTKIHKRLIDILNSTSKTVDALMKLDLPAGVDIEIKV
                     "
BASE COUNT         6392 a         5483 c         4887 g         7391 t
ORIGIN      
        1 tattaataaa aactgtccaa aattcgctaa tcccgaccgg aaaaacaaaa ttaatccaga
       61 atgtcgaatt tggcagaaaa ccggtttatc ctgcttttta atacaaagac tgggatttga
      121 gcgggtgagg agaatcgaac tcccgtgacc agcttgggaa gctggagttc taccactgaa
      181 ctacacccgc aatgcaaaaa agcgttgata atttaatagc actaaataat tttaaaaatg
      241 tttttaatta cttgattgga taaacattac ccagtctatt ttaaagtttt tgtcaaggta
      301 attagcattt atcggctaat gcttattatc caacaatttg tgtttttctc gtcgagaaat
      361 acaaaatctt gtgtttttcc ttgactactt ggtatgaact tactaacttt cacccagttt
      421 aacttttgaa aattaaaatg ggctcaatca attaactcaa aataggagat tttcgcgaat
      481 gtctgagtcg attaccatag attttaaaaa tcccaataac gaatcgcccg aaaagaatat
      541 tccagaaaat tctctgtcaa ttaatcccga aaatattcaa gttactcccg agaagaaacc
      601 tttattttcc aaaaatgcga tggaggtatt aaccaaacgc taccttcagc acgataaaaa
      661 tggcaatgcc gtcgaaacgg tcgaggaatt attccgcaga gtggctgaag cggtagccga
      721 agccgaggct aattactctc cgcgctccag cattccccgc acaaccgaaa aattttacca
      781 gatgatgatt cgtaaagact tcctccccaa ttcaccaacc ttgatgaatg ccggtcgtga
      841 acttgggcag ctttcggctt gctttgtcct accgattgat gattcgatgg aatctatctt
      901 tcagacttta aaagacaccg cactaattca taaaagtggc ggtggaaccg gcttttcctt
      961 cagccggatt cgccccaaaa aaagcattgt aaaaactacc agtggggttg ccagcggacc
     1021 agtatccttt atgaaagttt acaacgccgc cacggaagcc gtcaaacaag gtggcacccg
     1081 gcggggagct aatatgggaa ttctgcgcgt tgatcatccc gatatcctcg aattcgtaga
     1141 ttgcaagaaa aatagcaatg agctaactaa ttttaatatt tcggtcgccg taaccgatga
     1201 atttatggaa aaagtccggc gacgcgaaca gtatgaactg gtcaatccgg ttgacaatac
     1261 tatcgtcgga actcttaatg cggctgaagt tttcaaactg attgcccaga atgcctggta
     1321 caacggcgat cccggtataa tttttattga ccgtattaat cagatgaatc ccactccgaa
     1381 attaggcgat attgaagcca ccaatccttg cggcgagcaa ccgctcctgc ctaacgaatc
     1441 ctgcaatctc ggttcgatca acttagccaa ttttatttcc gagaataaaa ttgactataa
     1501 gcgccttggc gagactattg atttagccgt tcaatttctg gacgatgtaa ttgacatcaa
     1561 taaattccct ctcaagaaaa tcgaaacaat ggtaaaaaag acgcgcaaga tcggtctggg
     1621 agttatggga tttgccgacg ttcttttcag cttgaaaata ccttacgatt cggatgaagc
     1681 acttcagtta gccgacgaat taatgaaatt tatctacaac caaaccaagg aagcctctac
     1741 caaattaggc gagagtcgtg gtacttttcc tgcctggaac gatagcattt acttccccga
     1801 tggtcccaaa tatcgcaatt caacctgcat cacgattgca ccaaccggta caatttcaat
     1861 gattgccgac tgttcgagtg gcattgaacc gattttttca ttagcattca ctaaaaaagt
     1921 tatggatggg accgaactgc tttatgttaa tccttatttc gaacaggctt gcaaagaagc
     1981 cggtatatac agtgcagaat tgatgcatcg ggtcatcgcc gaaggctcaa tccagcatat
     2041 tgcagaaatt cctgagaaaa taaggcgggt tttcgttacg gcacatgata tttcacccga
     2101 atggcacgtt cggatgcaag ccgcttttca gaaatgggta gatgcggcgg tttccaagac
     2161 ctgcaacttc cccaaatcgg ctacggtgca ggatgtcctg gacgcttatt ggctggctta
     2221 tgaagccggt tgtaaaggca tcacaattta tcgagacgga agtcgggacg aacaggttct
     2281 gtacgtcggc aaacagaccg atcaggctaa atcggagcaa gccgttcagc ccccgataat
     2341 tgaccaaacc ctgcatccgc gaattcgtcc acccgagact catggagtaa ccaaacgcgt
     2401 tcggacgggt aatggcactt tgtatatcac gatcaatgaa gatgactacg ggctgtgtga
     2461 agtctttacc aatatcggca aagctggtag tcaggcgcaa gtcgaatccg aagccattag
     2521 tcgtttgatt tcgcttgccc tgcgttctgg aattgacgcc cgcgatgtgg ttaagcagtt
     2581 aaagggcatc ggtgggcctt cgccgatctg ggaaaacggc actcaaattc tctcgactcc
     2641 ggacgctatt gccaaagccc tcgaatggta tctggacgaa cgcgaaggca agaagaaaac
     2701 cactggcaac caaccgagtt tttcgctact cgacgttgag agtacaacga cggtaaagaa
     2761 gcccgaacct acttcacaac acaccgcgcc ccataataaa aagtcaatta cgacctgtcc
     2821 ggaatgtggt tctaccgttt accatgaaag tggctgtgtg acctgcccga attgtggctt
     2881 ttcgaaatgc taaactgcgt ctgggtttag aatctatact ttgatacata ttttcatata
     2941 cctccaagct ataccgaaag cctaaggtca ccgagcgatt aaatattttg cgtatctgcc
     3001 atgtgaattt gaaaactcga aaattctatc tgccttggtc ggtattaccg tttatcgtat
     3061 tttatttttc aatagcctaa tccgtcgact aatcttatac aatatttttc tctccttgcc
     3121 cctcattaat tcaatgcaag gaatatcagt ataattcttt agctgataat ggattacaaa
     3181 ctaaaattag cgatccgatg gccagtgata acttccaagc caatatcgct ggcgcgttca
     3241 ataaagcccc gatgcgggcg atgggacatg aaaataaatc ctttagcttt caatatccta
     3301 acagtttcat gaatagcctg aagttcaata aatgactcgc gggtgatggg ataggagaaa
     3361 cattgaaaaa tataaaaatt acaccctttc atcatacaga tatcaatcat tgattcacaa
     3421 cgcattgctt cagggacact ctccctgtca atccgcacat tcagacggac atcatcgccc
     3481 tgacggtgca ccctgagaaa taccaattca cgcaaccacc caccattgaa aaggtaatta
     3541 ccgccgtcgc cggcaatgtc aacagttttc tggttttcgc ccgttccaaa gcggaagcgc
     3601 ataacattct gttccgcatc ataattaaca acgaaacgca atcgctgatc atcaattttc
     3661 caatgaaccg tctttttatt ttcgcccata ttattccatt cgtctcgatg cgaatcaata
     3721 aacggcatga tccgatctag attattgccg ataaaaaatg aaagggcgct acgttttccg
     3781 atttccggat cgaagcgcgt cccggattcc attttaattc cgtgcagagc aatatattca
     3841 tttacagtta gattaaattg aaaagtctta acctcgccgg ttttgacatc cacaattcgc
     3901 gagttgtcgg gatcaatcag gatgacttcc ttaccggcat ttttaaaaac ttcagccgcc
     3961 agaacagcat gaagacgcga tcccccacta atattcagaa taacatcatg ttcaggatag
     4021 tctctgagaa ctttctggca ctgttcgata aattcgggaa catcattcgg attgatttca
     4081 cggctctcga atgtcgagct aatttgtcca cttaaagcag tttcgattag cgaaacgacc
     4141 tttttctgat ttggggtgcg gagccaaaca atagcgtccg gcttttttaa aattgcactt
     4201 agatatccga ttatcggtaa atcgtttacc aaacaaactt ccaacagcgc catattcatc
     4261 tcccatttct actaaaacga atcatttatt aaacattttt agaatttaat aattaatttg
     4321 agtttttcaa taagtgattt taataaaaga agataggctg gtaaagcgag taattatccg
     4381 aattacctta ccctgaaaaa ttaaaaggtg atggaattta atttagggct aaatcattct
     4441 ccgcaaccag cgcttccgcc ataatgtctc ttacacaggt aggtggtgcg ggtggcttac
     4501 ctgcttgtcg gtaatgaaat tctttgccct cgtaattgca cataacgact tcttcgtcag
     4561 aaatggactt gacatattcg ggtaggagag ggatttttcc gcctccaccg ggagcgtcaa
     4621 tcacgaaatg cggaaccgcc agaccggaag tccagccgcg cagagctttg ataatttcta
     4681 aacctttctc gactcgggtg cggaagtgtt cggtaccata gaccatatca gcctgataaa
     4741 tataataagg ccggacgcgc gcttggagca atttctgcat cagtaatttc atcacttcag
     4801 ggtcatcgtt tacccctttc aagagtacgg tctggtttcc gagaggaata ccagcatcag
     4861 ccaggcgccc aagagccttg accgcttccg gcgtcaactc atccggatgg ttgaagtgaa
     4921 cgttaatgta tagtggatga tatttcttca ggatccgaac taatttcgta gtaattcgct
     4981 gaggcagaaa acagggtacg cgggtgccaa tgcgtagaat ttgaatatgc ggtatttggc
     5041 gtaggcttga tagaatacgc tcgattttct ggtcactcag catcagcgga tcaccgcctg
     5101 aaatgatgac atctcgaatt tcaggatgtt ctgcaatgta tttaattcct tgttgaataa
     5161 agcgcggact gatcttctgt gcgtcaccga ccttgcgttt acgggtacaa aaacggcaat
     5221 atgaggcaca ttccggagaa acaagaaaaa gacagcgatc gggatagcga tggacaatac
     5281 tgggtaccgg cgaatctttt tcttcgctga gcggatcgta ttccaagccg caatcttcca
     5341 attcagcctt gtcaggtata acctgcctgt aaatcggatc gcctttcttt ttaatcaatg
     5401 aaaaataata tggattgacc ttgatccgaa agactttgtc aagcccagcc aactcgtcat
     5461 ttggaaggtt taaggctttg gtgatttgat ccacagtagt gtaacttttt tggaccaatt
     5521 tgcgccattt ttccatttct tatttattct cctgcttaaa gtattttaca aaaattactc
     5581 ggtcatcacc ccggcgataa aaatctttaa tttgggcggc aattgcataa ccatttttca
     5641 gataaaaatt gcgggttggt aaatatttct cctgggagga tgtttcaatt ataatcagat
     5701 tgccatgctg ggcttggaca gcggtttcta cataagtcaa caagtctgtg ccgatgcctt
     5761 tgccctgaac ctggggagcc actgcaatcc agtaaagatc aaaagtaccc tcagtagcag
     5821 gggtaggccc atagcaaaca taaccggcga gtttctggcc attgatggct acaaatatta
     5881 aataatcagt ttggttaggt tttgtcaaag cggtgtcaat caattccata gccaccctaa
     5941 tttccgccgt agtaaacatc tgggtatcgc ttaatatttt cagaatctcg gctcgatcgg
     6001 tggattttat ctcgcgaatc atgttcatct tcttcgcctg ttcaaagcct gttttataag
     6061 aaaaacaaca aatgtttcgt aactgatacc ggcggcattt aaagcccgaa caaaaccagc
     6121 agaaggcgaa atatcaggat tgggattata ttccagtaca aataaattac cggcggcatc
     6181 catacgaata tcaattcgtc cataatctcg accacgcaaa gcgtgataaa ccgctaaact
     6241 aaggctggtc aatttttcgt taagttcggc actaatcgga gccggacaaa ccggtggggt
     6301 catttgataa acggggtgat cactgagcca cttagcttcg tagctagtga tgggaaaata
     6361 ctcttcgctg acctgttcga aattaatttc agaaggtggc aaaaccagtg gattttcatt
     6421 gccgagaata ctgacattga attcgcggcc ggctatatat ttttcaacca aaattggttg
     6481 cttatactta gccagtaact ctccgacgat cgactttaat tcaatcagat cattgacgac
     6541 tgaattctga gtaataccga tactaccatc ttccctcgaa ggcttagcaa tcaatggaaa
     6601 atccagactg caggtttccg gaatagattc gtagacgtaa aaatcaggcg tgggaattcc
     6661 tatggcttta aaaatatttt tcgataacac tttattctgt gccagaccaa gcgttagagg
     6721 agaattaccg gtataaggca aatctagcat ctcccagagt ccggcgacat tcatttcctg
     6781 gtcggtgcga ctttgccaac cttcacagag attgaaaatt acatccggct ggaaggagtt
     6841 aatttttatt aaatcctcat caatgtttaa aatcggaagc atttcggcta cgtaaccgca
     6901 attattcaga gccgtataaa cggcggcagc ttcatctcta acggcgattt ccgaaataat
     6961 gtccggatca aaatttgaat ctaccgattg caaatcgttg aagaccacca gaattttgat
     7021 cttctgagat tcggatgaca ttaggacaag ccgtatctcc gggcggcgac ctgaacgact
     7081 tggtttaaca ttgcaggata ggtataaccg gcagtgcgcg cggctttggg aaaacaggaa
     7141 ttatcttctg gattaggcaa gatccccggc aaaggattga tctctaaaat atgggcgacc
     7201 ccattttcat ccagacggac atccaatcgg caccagtctc taatatttaa aatccggcaa
     7261 gttctacgaa caagtagttc gatttgattc ttcagattat catcaatttg agccgggcag
     7321 gtgaaaatat ttagcggttt ttctggccga tcccaaatcc atttggcttc ataggagtag
     7381 atcggttgag caccggtcgg caattgtgaa aaatccatac cgataatcgg cagaacttcc
     7441 atttcggggc tattgcctag gattgcgacc gtaaattccg gcccagccag gtacttttcc
     7501 atgattaccg gttcattatt atatttagca aaacaacttg caattacgtc atcagcttgt
     7561 tcttttgagt aaaccaccga attatcaaaa atgcctttgc tactaccttc tgctaacggt
     7621 ttaacaatag ccggaaattt aaaattttcc gcaaaaccat cgtccccggg atatatgacc
     7681 tgaaaggtag cattcggaat gtgataatag ctcaaaattt ctttacaacg tgctttattc
     7741 agacaaattg ccagtgttaa aggatcggaa ccggtatatg gtaaacccag cctttcgcag
     7801 attaagggca cataggattc gcgccaatcg ccaaatagcc cttccgtaac attaaaaacg
     7861 atgtccgggc ggctttgttc taaaccgtgc gcgacctcgt catcaccttc aaagctgact
     7921 acctgatgat tatttaattg gagagcatct acgattgcct caatcgtttg agggtcatca
     7981 ccctcggcaa aaaggtcggg aggaatgtct tcgtcgtccg gatcgacggc aaagcgctta
     8041 cgatactcct ttctcaaacc ttcttttgtg ctatacgtta atgcaatttt cattttttat
     8101 ggcgtgaaat ttacatatta tttttgaaat gcaagtaaaa tatttttata aattgaaatt
     8161 tctttaaaat tctaaaaagc ccaatccttt ggggaatgct attttaggat tacaaattgt
     8221 tgtatagaaa aatatctcca acctaacgcg ggtgggcaat ctcaaaggct ttacggacaa
     8281 caggatagta ttgcgggtct ttcaaaaagg tctcgtaaga gaaaaatact atattttgaa
     8341 attgattatt taaagttaat atcgttttag tcatagcctc gtattgactc tgattataga
     8401 cactaatacc gacccagaca ttatttttat ttatattagg gtcaattttc ctcaggattt
     8461 tttggaattg cttagtatct ttggtatagt tcatcaagac cacgaaatcc agccagtctt
     8521 gccttaacca atattgccag tcttgaaaga aatttttaca ggcatcgtcc ggatcgggct
     8581 tgacggccgc acttaattgt agagacggtc gaatttctcg taaattcttg cgggcattat
     8641 gaacgaaatc agaaatggca tctcgccgaa agtcttccca tttgagcatt aaagaatcaa
     8701 tttccgccag ttctaatcct ttataaagtg atttgtcgga gagggttaag aggagaggat
     8761 caattccata tttctcacga aataatatgc ggccaatagg attgtaatcg tagcaatttt
     8821 ttgcataacg aacgtaatca agatgaatgc catcgaggtc ataattggtg gctaattcct
     8881 gaataatatc aatgaggtat ttacgtactt ctggtaagag gggcgataaa tagattcctt
     8941 cggtacccaa ggtgttgaaa tcagtggcac ggcgttggat atcttttata ccattggcat
     9001 ctaccgtaca ccactcggga tgttggtaca aaaggtgatt ttcatttttc ggtttctgat
     9061 cagatgacca tagcaaaaat acattaaccc aggcatggac tctcaatcca taactatggg
     9121 cttggctgag agtgtattcc agtggatccc agttattgtt gcttagcaac tcactataga
     9181 ctacaatttc ggagtgataa aaggcatcac cgcgaccacg aacttgaaca aacagatctt
     9241 ttatatcatt tagaacggcg aagttaacga aggcatctat tttctcttta gaattgagct
     9301 ggtcgcgaat tacccataag ccgaggttgg tctgggctcg agcggtgacc gttagactat
     9361 aaattagaag ggcagtcatc gcgactgccc tgatttttaa agataattga tttttcaaaa
     9421 acaatccaac aagtttattt actcagtctc cgatgcgggt tcggcttttt cgcctgatat
     9481 attttctggt gggatatctt cttttacttc tgcttgggct gattgttttt cttctttttt
     9541 ggcctgagtt tttttggtag gcttggtctt agctggagtt tgttcttttt ctacttctgt
     9601 tgatttttcc tctttcttcc cctgaactga tttagtcgcc tctgaaccgg ccttcttctt
     9661 tttttcttcc ggttgtttat aatctacaaa ttcgattaaa gaaacagccg cattatcatt
     9721 atcgcgatag cctagtttaa taattcgagt ataacccccg ttgcgatttg catactgcgg
     9781 agcaatgtct tcgaataatt tatttactaa atcgcgatcc tgaataaatc ggaaagcaag
     9841 gcgacgggaa tggacagtat ctctcttgcc ataagtaatc aagcgttcaa ctacgcgacg
     9901 taattctttt gcttttgcgt cagtagtttt tattctacca tttatcagca ttgccttcgt
     9961 tagactttgt attagcgcct tgcgctggtc agttgatcgg cctaatttag ctaccttttt
    10021 ctggtggcgc atatcagtgg tactcctttt gggttattct tctttctcaa cgaggtattt
    10081 gtcaacatcc atgccgaatt ccaagcccat gcttttcaat ttggtcatta actcatttag
    10141 agattttcgt ccaaaatttt tgaactttag catatcggct tcttcatggg aaaccagatc
    10201 ggcaatggta cggatgttat tggcttttaa gcaattgtat gatctaacgg ataactccat
    10261 ttcatccacg ctctttttga gcagattcct gattttaagg acatcttcgc tgggttcttc
    10321 acgttgaaat tcgatcggtt tagcttcgac gcgtgaaaaa aggctaaaga aatcgcgtag
    10381 aattttagag gctagatcga gggattcatc gggagtaatg ctatcatctg tcgtaatttc
    10441 cagggttagt ttctcgagaa tttcacgggc ggtaccgggc acattttcga ctttatagga
    10501 tacattaaga atgggtgaaa aaatcgaatc aatgaaaatc gttccaatcg gataatcggg
    10561 cagcttattc ttctccgctg gtacccaacc tgtgcctcgg gcgattctaa tctcaatatt
    10621 gaaatcggca tcctcattca tcgtgcagat atgtaaatcc ggattcaaaa tcttaaactg
    10681 ggtaatattt tcgccaatca ttccggccgt aaattcacca gcgcctttaa aatggagagt
    10741 gactttatct ggcttgggat cgaatagttt gaggcgaact tgtttcagat taagaattat
    10801 ttccgcaaca tcttcacgta ctccaggaat agtggaaaat tcatgcaaaa cgccatcaat
    10861 tttgatcgaa cagattgccg cacccggtat cgaagtcaag agaacccgtc gcagggcatt
    10921 accgatggtg atgccaaaac cccgttccag cggctgtaga taataaatgc catgagtgtc
    10981 attagaattt tcgtctttaa cgactctggc gattatttga tcgtaaacag tcattatttc
    11041 cctccttatt tagaataaag ttcgacaacc aattgttcgt tgacatcaac gttcatctga
    11101 tctctttccg ggacttggat gaaaattccc tgcattttgg ctttgtcaag aatcaaccag
    11161 ggtagttcgt gctcgccacg gattcgtttc atcgaatcat gaattacctg caatttcttg
    11221 cttttttctc taacctgaat gacatcacca gctttgagtt gataggaagg gacatcaacg
    11281 acgcggttgt ttacggtaaa atggcggtgg ctgactagct cgcgtgccat ccgccgggat
    11341 gaagcaaagc caagccgata gacgacatta tccagtcttc tttccagaat cttcatcagg
    11401 ttagtaccgg tgacgccctt ctggcggttg gctaattcga aatattggcg gaactgtttt
    11461 tctagaacgc cataaattct ctttattttc tgtttttcac gtaattggat tccataattt
    11521 gacaaacgtg gatgagcagt ctgactgcca tgttgaccgg ggatataact tttcgtttca
    11581 aaagagcatt tggtgctgtt acagcgttcg cctttcagaa aaagtttttt accctcccgg
    11641 cgacatagtt tgcatactgg acctgtatat cttgccatta aatattctcc attttagaaa
    11701 ttatactctt ctccgctttg gaggccggca accgttgtgc ggtatcgggg taacgtcctt
    11761 aatagctgaa atttgtagtc cggctaaatt cagcgctcgg atagcggctt ctctgcccgc
    11821 gcctgggcct ttgactcgta cttcgacgcg ctgtaatcca agattaatgg cttcttgagc
    11881 ggcttttgtg gcggcttgct gggaagcgta gggcgtgttc ttgcgcgagc ctttgaaacc
    11941 ggctgttcca gctgaagccc aggaaattac attaccatag ttatcggtta gtgatattat
    12001 cgtattgtta aaggttgctt taatagtagc aattccttcc gattcaactt ttgtgcgttt
    12061 cttctttttt acggtagaac gtttgggtgg caaatcaaat acctcctatt ttatcctttt
    12121 ttcccgactt ttactttctt acggctaact gtgcgccgtg gtcctttgcg cgtgcgagca
    12181 ttggttttag tccgttgacc gcgaaccggc aaacctcgac gatggcgcaa gccgcgataa
    12241 caaccaatat ccattaggcg tttgatgttc atctgaactt ctgtgcgcaa agtaccttca
    12301 actttatatt cattgctaat cacattgcgg atatcgcgca cctgttcttc ggttagcgct
    12361 tttacccgga tttcaggatc aactcccgct ttctgcagga tgtccatagc attagcccgg
    12421 ccaattccat agatgtatgc caaggctatt ttcactttct tttcgttcgg taaatctaca
    12481 ccggcaattc gagccaaatt ttcctcctaa ttgcttttat ccctgtcgct gtttatgttt
    12541 tggattggta caaataactc gcactactcc tttgcgacgg ataattttac atttgttaca
    12601 aattttctta actgatgatc ttactttcat tttctatatc caatcttcat ttatagcgat
    12661 aagttatgcg acccttggtc aaatcatagg gtgatagctc catggtaacc ttatcacccg
    12721 gtaaaatctt gataaagtgc atccgcattt taccggaaac gtgcgccaga acttcatgtc
    12781 cattctcgag cactacccga aaggaagcgt tcggcaaatt ttccttaatt attccgtcaa
    12841 cttttatcaa tttttctttt accacttttt tttcctttta ttttctttgt gataaaattt
    12901 gcggtccatc cggaccaata gcaatcgtat gttcgtaatg ggctgaaggt aaatgatcct
    12961 ccgtgattac cgtccaacca tctttcaaca aacggacttc ataacttccc atcgtaatca
    13021 tcggttcgat tgccagacac atacccggtt tcaaaactgg tccgcgaccc ggttcaccgt
    13081 aattgggaat ctctggcggc tcatgcagtt cgcgacccac cccgtgccca accagttcac
    13141 gcacaactcc ataaccgtag ctctcgacgt gggtctggat ggcatgtccg atgtccgata
    13201 aatgatttcc gggtcgagcc tgagctatgc cttgatagag tgaatcctcc gtgacgcgca
    13261 ttaattgtaa tcggtcatca gagactgttc ccacggcaaa ggtcatcgcc gcatcgccat
    13321 agtagccatt tttcttgaca ccgacgtcga tgctgataat ttcgccctct ttgagaactc
    13381 ggttggaagg aattccgtgg acaacttgct catcaatgct ggtgcatata ttgccgggaa
    13441 atccgttata gcccttgaaa gccgaatgcc cgccttggct cttaataaat tctgccgcca
    13501 gatgatcaag atagagcgtc gttataccag actttatctc tcgacttaac aaatcgagag
    13561 tttcagagac aatccgacaa ctttcacgaa tcttctcaat ttcctcggct gtacgaatat
    13621 gaaccattat cgctaattat ccgcggcggc cctttaattt tccgcttttc aaaaaaccat
    13681 cataatgtct catcaaaaga tgagattcta tctgctgtaa tgtatccaaa gctacaccga
    13741 cgacgatcag gagacttgtt cccccaaaga aagaagccag atcataatta atgtccataa
    13801 tcttcatcag tatataggga aaaacagcaa tgaatgctaa aaagatcgag ccgggtaacg
    13861 tgactttggt tagaatatta tcaataaact ccgccgtctt tttaccaggt ctaattcccg
    13921 gaataaaacc gccatacttt tttagattat ctgcgacttc taccggatta aaggcaatcg
    13981 ctgtataaaa ataagtaaaa aagacgatta aaactccata aatcagccaa tatacaattg
    14041 attgcacact aaatattcgc atcattccct ggacgaaatt actgttcgga aagaaagtag
    14101 caatagtgct cggaatgaat acaatcgact gcgcaaaaat gatcggcatt acaccggcgg
    14161 tattgacccg cagaggaatg tgagtcgcct gccctccgta aactttccgg ccaacgactc
    14221 ttttggcata ttgtactgga atacgacgtg tgccttgggt caatagcacc acaaatccga
    14281 caattactat cattaatacg attaaaatta tttccgtaaa taatccgcgg acaccttctc
    14341 gaatcagagt aatttcgctg acaaacacgt ttggtaaacg cgccaggatt ccgaccatta
    14401 taatcataga tgtaccgtta ccaatacccc tttctgtcat acgttcacca agccacatta
    14461 gtaaaaccgt accggttgtt atagtcagca ttgtcattaa aacgaaacca actcccggtc
    14521 ggggtacaac tggtagcatt tgattgccca cgcgggctga catctgagga ctcatcagaa
    14581 attgagctac tccaaatgat tgcagggcgg caataataac cgcaccataa cgcgacatct
    14641 gggtcaattt ttttcgccct tcttcaccct ctttttgtaa tttttgaaaa tatgggatta
    14701 ctgcaccgag caattgtaag ataattgaag ccgtaatata aggcattata cccagcgcga
    14761 aaagagtcgc ctgagcaaat gcccctccgg agaacatatc atacagtcca aagagtgtat
    14821 tcgcgaaact actcattgcc gcacccagta cttcagtatc aatacctgga atcggtatat
    14881 gggctccaat tctgaaaact atcagaaggg caagggtata accgatgcgt tttctcaatt
    14941 cgggaatctt aaatatactc tggaattgct ggatcataaa agttctacct ttcctccgga
    15001 agccgttatt ttctctttcg ctcgggtact gaaggcatgc gcaacgacgg taatagcacc
    15061 agagagttca cctcgcccta atatttttac cggttttttt aggcttttaa tgaggccttt
    15121 ttgttgcaga atttccggat tcacttcggt tatttgtaaa cgagcaatat gatccaaatt
    15181 gactacttga aattcttctt tgaataaatt tttaaaacct cttttgggta aacggcgata
    15241 gattggcatc tgtccaccct caacatagac gggtttttta ttgctacccc gtgcaccagt
    15301 tcctttatta ccgcgaccgg ctgtaccgcc gtgtccggaa ccaggtccac gtccgaggcg
    15361 ctttttattt tttactgaac cgtaagccgg tttaagtgtg tttaagccca tatcctattc
    15421 cttaatctct tctactttta gtaaatgcgg aatggagttg atcataccgc ggattgccgg
    15481 attgtctggt aaaagaaccg tctgattcat tttagtaatt cctaaggcaa gcaaggtgcg
    15541 tttggttttt tcacagtagc cgattccgct ttttacctga gtaattttca gcattgcagt
    15601 tttatctttt tttgtcatga accaaatatc tcctgaagcg ttttttcgcg tctatgagcc
    15661 accatgtagg ggtcttttaa tttcgagagg gcaagcattg tcgctttgac tacattatgc
    15721 gaattattag agccgagcga tttggataaa acatcgcgca caccgaggct ttccataata
    15781 gcccgaatcg cacccccggc gataacgcca gtaccaggag tagccggacg cagaatgact
    15841 ttggcggcgc cgtatttagc ctggacttcg tggggtatgg taccgttgat gaccgggatt
    15901 tttaccaaat ttttcttggc gttttctttg cccttagcca ccgctgaact cacctcgagg
    15961 gctttgccca gaccaatccc gacgtgccct ttgccgtcac cgattgctac cagggcattg
    16021 aagcgaaacc ttcgtccacc ggtaacaacc tttgcaactc gattaatttt tatgactttt
    16081 tcttccagta attccagttg agttggatct atagattttc tcaagttacc attcctccgc
    16141 tattagaatt ctaatccggc ttgacgtgcc gcttctgcta aagcttttac gcgaccatga
    16201 tattgatacc cgctccggtc aaaaactact ttagtaatac cttttgccac agctttttcg
    16261 gctaaggttt tacccaccag agcacttcgg tcggactttt tagtattgct tttgatctgc
    16321 tcgcggactt caggcgtaag gtctgaaacg gcgagaagtg atttatgaag gttatcatca
    16381 acaacctggg cataaatatg attcaggctt cggaaaaccg tcagacgcgg ccgctccgac
    16441 gtaccttgta ttttgtgtcg gttacgtttc tggcggcgct gtcgagcgaa tgcttttata
    16501 ttcttgactt tattcatgta taaaacctct atttcacacc aactttttta ccggcttttc
    16561 tgcggatatg ttcattctgg taacgaattc ctttaccctt ataaggttca cacggtttga
    16621 tagatcgaat ttttgccgca atttgtccga ctttttgttt atcaatacct tttacgacaa
    16681 tagtaagggc tttgggaacc tcaaagctta ccccttcggg aggcgtaact aagattgcat
    16741 gggaataacc aacattaatt tgaacggatt tgcccttcat ctcagccgaa tagccgacac
    16801 cttcgatttc cagagtcttg ctgaatcctt gcaatacacc ctggatcata ttagcgatca
    16861 ttacccgcgt caagccgtgt aaagctttat gattgcgact atcgctcggg cgggtaacaa
    16921 ttatctgact atcgtttatc tgaactttca tatcggacgg aaaatcccac tttagttcac
    16981 ctttgggacc tttgactgta atgctacaat cttggattgt aacattgact tctttaggaa
    17041 tttcgattgg ttttttaccg attcgtgaca ctctatactc cttgaatcac ctttttacca
    17101 gacatagcaa agaacttcac cacccacatt attttttcgg gcgactttat cggtcaggac
    17161 gcccattgga gttgataaaa tagctattcc cattccacca actacccgag ggatattatc
    17221 cgaagccgca taaacacgcc gaccaggttt gctgattctt tgcaaacctt cgatcatcgg
    17281 ttcaccagtt ttagaatagc gtaagtagat acgaatgagt ccctgtttgc cgtctttgat
    17341 taaaataaag tccttgataa aattttcgtg ctttaaaata agcgatattc tcttctttaa
    17401 attggaggca ggaatatcaa cccagcgatg gttcgcggct tgggcattac gaatacgagt
    17461 taaataatct gcaattggat cggttaccgc catatcatat actcctgtat taccagcttg
    17521 cctttgtgat acccggaatt tcaccgcgca gggctaattc ccgaaaacaa agccggcaaa
    17581 gaccaaattt acgataataa ccgcgcggac gcccgcaaat cgaacaacga ttgactctcc
    17641 gcgtactgaa tttgggattt cgcctggctt tggcaataag tgatttttta gccatcttag
    17701 tttatctcca ttgcggtctt gacttcaccg ccggttgttg attctttgat tgctcttttc
    17761 ttgaacggca acccgagaag ggatagcaat tcatacgctt ccgtatcggt tttagccgtg
    17821 gtagtaaaag taatgttcat tccccgaata tgatcaactt tatcataatc tatttctgta
    17881 aaaataatct gttccttgag acctatcgaa tagttaccgc gaccgtcgaa agatttatca
    17941 gaaagtcctg aaaagtcacg tactcgtggt aaagctgtcg aaataagccg atccataaat
    18001 tcatacattc gaaaacgacg cagggtaacg gcacaaccga ccggatctcc agttttaatt
    18061 ttaaaattgg aaatggcgcg ttttgcttta gtcaccatcg gttgctgtcc agtaataata
    18121 cgaatgtctt caactgcttt cttcaagcta gctggatctt ccttggtttt gcccactccc
    18181 atatttatgc ttattttgac aagtttggga acctgcataa tatttttata gccgaatttt
    18241 ttcaccatag ctggaactat ttcggtctcg tatgcggtca ttaagcgggg tttatatgca
    18301 gacttttttg ccacttatag ctcctaatta tatagcatca ataatttcac cagtttcgcg
    18361 tgctacgcgg acttttttcc catctttcag agtccgatag ccgactttag ttgcctgatc
    18421 ggcatgtact aatttgacat tagatatatg gatcggcgct tctttttcaa taataccacc
    18481 ttgaggattt tgctggttgg ggcgagtatg ccttttgata aaatttactc cttccacgat
    18541 tactcgttct ttatccggga aaactttcaa aacctttccg attttgcctt tggattctcc
    18601 cgttataacc attactgtat catttttacg tatgtgaagc atgtttaaat tcctttataa
    18661 aacctcgggt gccatagaga caattttcat ataatttttc tcgcgcagtt ctctggcaac
    18721 tggcccaaag attcgggttc ctctcggctc aaggttattg tcaatcagaa cagcggcatt
    18781 gtcatcaaag cggatatatg agccatcttt gcggcgaact tccttggtcg tgcgcacgac
    18841 gacggctttg gatttttcgc cttttttaac cataccgccg ggcgtagcct gttttatggt
    18901 tacaacgatg gtatcaccga ctgttccgta tttacgatgg gaaccgccca ttactcggat
    18961 gaccattact tcttttgctc ccgtattgtc ggccactttt aatcttgatt cttgctgtat
    19021 catagaaata tcctttgtgg tttgtcctag cgttcaatta tttccagcag acgccatctc
    19081 ttcaaccggc ttaagggacg cgattcgaca attttaactt tatcgccttc gtgacattta
    19141 ttctcggcat catggacata aaatttggaa gtccgtcgaa tatatttgcc ataagtgctg
    19201 tgctttacag ttcgctcgac ggatacgaca gcggttttat cttgcttatg actgatgact
    19261 gttccaatta aaatttttct tctgtcgctt gaaatcattc ttgattgtcc tgtgatttat
    19321 ttattcggtt tatcttttga tctgattccc agttcgtact catgtaaaac ggttcgaatt
    19381 tgagcaattt ctctcttgac gtgcctgatc ttcagtggat tttctaattg ctgaagagct
    19441 ttttgaaagc gtaggttttc caaggcggtt aaattgtcgc gtaatttagt ttccagttca
    19501 ttcaccgtta attcatcaag agtacttttt ttcactttat gctcctaatt ctcttctagc
    19561 tacgactttg gttttaatgg gcaatttatg ggtagcagtc ttgaatatgt ctttagcttc
    19621 ttgttcgctg gctacttcaa tttcgaataa aatacgtccc ggttttacca cggcgaccca
    19681 gaactcaggt gtccctttgc cttttcccat gcgggtttca gctggttttt tagtgatagg
    19741 tttatccggg aagatccgaa tccacatttt accatgtttg cgaatttggc gcgaaattgc
    19801 tactcgaaca gcttcgatct gccgactatc aatccagccg ggttccatag cttttaaacc
    19861 ataatgaccg aaactgacgg cattcccacg ggttgcgatt cccgtcatcc ggcctctttg
    19921 ctgttttctc cattttatct ttttcggtgc cagcatgatt aattattcct tttttaactt
    19981 ttcttgaatc tctcgccgtt acaaatccag actttgattc cgatgcaacc gtaagttgta
    20041 tgcgctgtta cagcggcaaa atcaatatcg gctcgcaagg tatgtaaagg aacccgtcct
    20101 tcgcgcatag tttctgtacg ggcaatttcg gcgccgccta accggccact gcattgaacc
    20161 cggattcctt ccgcacccat acgcatggtt gattgaatgg ctttttttac agcacggcga
    20221 taagatactt ttttcactat ttgggcggct atattctgac caacgagtga ggcatccagt
    20281 tcgggacgct tgatttcatt aacattgatc tggacatcgg ttnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nntgaggcat ccagttcggg acgcttgatt tcattaacat
    20461 tgatctggac atcggttttg gtgactttct gtaattcgag tcggagtaat tcaacttctt
    20521 cgcctttttt gccaataaca atgccgggtc gcgaggtatt aatggtaatt gtgatttttt
    20581 taggagtccg atagatatcc actcttgaaa tgccggcatt aggaagacgt ttcaaaacat
    20641 aacgccgtaa tttgatatcc tctgttaatt tttcagtgaa atttctttcg tcaaaccagt
    20701 aactgctcca ctccttgttg aatcccagac gaaaacctat tggatgcgtt ttttgaccca
    20761 agtcacctcc gattattgta ctttactttt ttgtttattt tctttttccg ccacaactac
    20821 agtaatatga cagagccgac gcctgatgat actggctcga cccagtgatc cagcccgaaa
    20881 gcgtttagca atcgagccgc catctacgta gatagtcttt agatagagat cttccggatt
    20941 taatttcgaa ccgccctcac tattcaagag gttggacact gccgaccgaa cgaccttttc
    21001 tacgttctct gaggcttttt tactattaaa gtgtaggagg tttaaagccg tttcaacgtc
    21061 tttcccccgt actaaatcag caaccagacg gactttccgc gctgattgcc ttacatatct
    21121 atgaactgct ttggcttcca taactcgatt taggctccct tcttttccgc accggaatgc
    21181 ccgcggaaga ttctggtatg ggcaaattcg cccagcttat gacccaccat attttcggta
    21241 acgaagaccg ggatgaattt attgccgttg tgtacggcga aagtgtgacc gacaaactcg
    21301 ggagagatcg tagtgcgacg tgcccaggtt ttaatcactc gttttttccc ggagctattc
    21361 atttgctcga ttttcttcat caatttaaca tcaatgtatg gacctttttt cagcgaacga
    21421 ctcatccaat attctcccta attatttagt tcgtctcttg ataataaact tattgctggg
    21481 attacgtttt ttacgtgttc gatatccttt tgataactgc ccccaaggtg aacatggatg
    21541 ccttccgccc gagctcttac cttcgccacc acccattgga tgatcaatcg gattcatagc
    21601 cacaccccgt acgtgcggac ggcgacccat ccaacgactg cgtcctgctt ttccaatact
    21661 gatattttcg tggtctttgt tacttacgac gccgatcgtg gcatagcatt cttcattgat
    21721 gagccgtatt tcacccgaag gcatttttaa agtcacatac ttgccttctt tagccataat
    21781 ttgagcggct gtgccggctc cgcgagcgat ttgccctcct tttttcggac gcatttcgat
    21841 attgtgtaca aaagtacccg cgggaatatt ttttaacggc agacaattac cgaccttaat
    21901 gggggcttct tcacccgaga ggacaacgct accggtaacg agtccatctg gtgcaattat
    21961 atagcgtttc tcaccgtctt tataaaatag aagtgcaatg cgtgctgatc gatttggatc
    22021 atactctata gctttgacag tcgcttcgat tccatatttg ttacgcttga agtcaatatt
    22081 ccggtaagca cgcttttgac cgccaccccg atggcgcgaa gttacccgac cgtaattatt
    22141 gcgaccggca gattgcgtaa ttggttcgag cagacttttt tcgggtctct ttttggtaat
    22201 atcttcaaaa gtatcgaccg tcttaaagcg ctgtccgggt gtaatcggtt tgatagtttt
    22261 aatacccatg atactatacc gccgtttctc cttgataaaa atctattttt tcaccctcct
    22321 tgagggttac aatggctttt ttccagtttg accgacgtcc agtagtacgt ataactcgac
    22381 cgccactccg gactgtcatc agttttgttt tacccataat attctgagta gcgacttttt
    22441 ctactttgac cccaaactta tcttcaacag cctttttgat ttcgattttg ttggcagagg
    22501 gatgaacttt aaagccatat ttattttggc tttcctgtaa ttcgttaatt ttttcggtta
    22561 gaattggttt gataatgatt gacttagaag acatatttag ttcctactat cgcccaacaa
    22621 ggcattattt aacttttcga agccgctttt atcgattaag agaacttcgc agtccagcag
    22681 atcgtaggtc gaagcttttt cagcttcgag gactaagaca ttgtaaaaat tgcggctggc
    22741 taacaataga ttagtattta aattagcggt tagaatagtc acttttttat ccaaaaggtc
    22801 aaattttttc agtaattcgc ggatatttct ggattttggc tcgtcgaagg acaattcctc
    22861 gatgattttt agcgcacctt cgcggtagcg gtaagccaga gcgctttggc gcgccagtga
    22921 tttgactttt ttgttaattt tcatcttata gggatgcggt ttagggccaa aagcccgtcc
    22981 accgccgacc cagaccgatg accgacggga gccaactcga gcccgaccgg tgcctttttg
    23041 tttccatggc ttcttcccac caccccgtac ttcaccacgg gttttggtag aatgagtacc
    23101 ctgacgacta ttagtcattt cggttttaac ggctaaataa atagcatggt cgtgcggttc
    23161 gatatcaaag gctggggtct cgattactaa tttctggcca gaatctgaac catctaattt
    23221 ataaacagaa aattcgatac tcaccgttcc tatctcctaa tagttagaat actgtttttt
    23281 gaaccaggta cggcgccttt gatataaagc aagttgcttt cggggtcaat tttaacaacc
    23341 gttagattat tgaccgtgca acgcttgccg cccaaacgtc cagccatacg cactcctttg
    23401 ataacatgcg atggataggc ggaagcgcct atggaaccgg gagcacgtaa acgatcactc
    23461 tgtccgtggg tttttgggcc accgccaaaa ttgtggcgtt tgacaacacc ttgaaaacct
    23521 ttacccttgg aaatgccggt aattttaacc ttatctccag gattgaagag cgcaacagtt
    23581 actgtttcac ctacctgaca aaatttagga tcgtaatcac gaatttctac cagatacttc
    23641 aggggagtaa tattattctt cttgaaaacg cccagattag gcttagccat tagacgttct
    23701 ttggcttcca taaatcccag ttgaaccgag gcataaccgt ctttttcatt cgttttcacc
    23761 tgaaccactt tgcaaggccc agctgcaata actgtcaccg ggatcagttc accattctcc
    23821 ttgaatatct ggctcatgcc aatttttttt ccaattattc cgatcataat gtatccattt
    23881 ataccttaat ttcgatgtcc acaccagctg gtaaatccag cttcatcaga gcatcaacag
    23941 ttttggaagt ggaattcaga atatcaatca gacgcttatg aatcttggtc tgaaattgtt
    24001 ctctggattt tttatccaca tgtggagaac gtaaaaccgt atataccgtg cgttgggttg
    24061 gtaatgggat cggtcccgag accatcgctc cggtggattt agctgttgct acaatctttc
    24121 ttgtactttt gtcaagtaaa ttatgatcgt aggcttttag actgattctg attctttgcc
    24181 caggcacagt tttactcctt attcaatgat ttcggtaacg acgccagcac caactgtccg
    24241 tccaccttct cgg
//
LOCUS       DAOMJU010000027        24215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_1925_length_24215_cov_18.547434, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000027 DAOMJU010000000
VERSION     DAOMJU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 24215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24215
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1925_length_24215_cov_18.547434"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PLO58_04175"
     CDS             complement(<1..1133)
                     /locus_tag="PLO58_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HQO74020.1"
                     /translation="MRTTIQLFALMLFIFSIGKICQADTTEVKQQIETIIAEIRKKYC
                     PDQRVALWKIEIDYQDNDYNLVGETDHPEAVRALTNQIKQTLPAIKIQNLIKILPDET
                     VNGKNFGIVINSVEPLRSGPSVFNDMVTQTLRGLTVEILKSAEGCFLVKTDDGYLGWM
                     EDDRLVVGGDSLKTAWENASKVVYDDFEGIIYSKADRKSMPVADVVLGNRFKMIGKQG
                     QWTRVEIPDGRRGFIPSQQLVSESEYLARTPTAEGIITTAKNLLGRPYLWGANSPKAF
                     DCSGFTQTVYRRNGYLLARDANMQVNQGFAVDTTNLLENLMPGDLLFFGPNPEQINHV
                     AIYIGDYEYIHCSAWVRINSLKPGTANYNDYLLKKLRYVRRIIG"
     gene            complement(1142..2788)
                     /locus_tag="PLO58_04180"
     CDS             complement(1142..2788)
                     /locus_tag="PLO58_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQO74021.1"
                     /translation="MKLSFKKGLKIWIAVLVSVSVLLAFTAKDIYKTISDSYKLYNAV
                     FQQILLNYADPLDAAALTESNIRNMTRELDPYTVYMTEEEKEPLEILSKGEYGGVGLR
                     ISLRNDTLTVISPIEGSPAKKANILPGDQILKIDTIWTTGLDLDKCTRLIRGNVGTKV
                     ELVIRRPGADEGKVYILERAKIQVYDVYYADYLEEGIGYIRLAEFSKGSTAEVRQAIE
                     NFKDHDQLEALILDLRGNPGGLLTEALTVAELFTQPGDTLLFTRGQNESSNRVDVARR
                     RPLLDESVKIAVLIDGGSASASEIVAGIIQDLDRGIVLGTPSFGKGLVQTVFRVDKEH
                     SLKITTAKYYLPSGRLIQKPDYIKNPKLIAASPSQDSIFYSRHKRQLKSKGGITPDII
                     IKSDEQSDFITELWRQNQFYSFAVKYKTEHNELPEEVNERIIKEFKNYIDHSGFQFIE
                     KTEKNIREMDKIISQDERLKNVKIDFQPVYEIFDSLKEAEFENNHLMIQRGIENQFAM
                     LRDGIAGQLKADLKYDKVVSEAILAVRDDGKYKTTLGLAR"
     gene            complement(2785..3417)
                     /gene="tmk"
                     /locus_tag="PLO58_04185"
     CDS             complement(2785..3417)
                     /gene="tmk"
                     /locus_tag="PLO58_04185"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQO74022.1"
                     /translation="MTKGLLITFEGIDGSGKTTQIKYFIKKCERENIPVALFREPGGT
                     PIGEKIREILLSKSNSEMFAITELLLYSASRHQLCRELIRPALESGKIVVCDRFYDST
                     TAYQGYGRGIDLNFINMLNRIAADNLVPDLTFIFDIPVAERLQRLGTRDLDRLEREEH
                     NFQEKIRQGFLKIAAAEPNRVCLIDGTKTEELVADEVWHKFNKIIRKMQI"
     gene            complement(3462..3872)
                     /locus_tag="PLO58_04190"
     CDS             complement(3462..3872)
                     /locus_tag="PLO58_04190"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQO74023.1"
                     /translation="MLEHHYQLRVRYSSVDQLGVVYYSRYLEYFEAARDEIMRAIGLP
                     YSQFEQAGYAMPVVEAHCVYHRSAHFDDLLTIISRISAMPTARLRIDYEILRENESQP
                     LVSGYTIHAFVNQSGKAVRPPQFFLKLLAAQMSK"
     gene            complement(3894..4874)
                     /locus_tag="PLO58_04195"
     CDS             complement(3894..4874)
                     /locus_tag="PLO58_04195"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQO74024.1"
                     /translation="MAEIVLDFEKPIIELEKKIEELRAQSAASGVDLNPEIEKAKLKL
                     NRLIQNTYSNLTRWQRVQLARHPERPYTLDYVKRICSEFIEIHGDRYYADDKAVVAGL
                     AQIEDYKVILIGQQKGRTTKENLYRNFGMPHPEGYRKARRAMKLAEKFKRPVISFIDT
                     QGAFPGVGAEERGQAEAIARNLFEMARLRTPIIVVVIGEGASGGALGIGVGDRILLLE
                     NTWYSVITPEGCASILYRDSSKAPLAAEAMKVTARDLAEFKIADEIIPEPLGGAHRNP
                     DQMAQTLKDVLLRYLAELTQIPIDELVDQRIEKYARMGSWKVVDVKSKSK"
     gene            complement(4882..6951)
                     /locus_tag="PLO58_04200"
     CDS             complement(4882..6951)
                     /locus_tag="PLO58_04200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO74025.1"
                     /translation="MQISVIIVSYNVKEFLQQCLLSLKNALEGLSSEIIVVDNDSVDG
                     TVNVIKNKFPEIILIENHFNKGFAAACNQGLAIARGEYILLLNPDTMLQEDTIKVMIE
                     FFRAHPEAGAAGCKILNADGSLQLACRRSFPTLTVALPKLLGLSRLFPKVKAFSRYNL
                     TYLDPDQITEVDAVSGSFLMMRRAVYEQIGGLDESYFLYGEDLDYCYRIKKSGWKIYY
                     VPDTKIIHYKGESAKHAAFDNFIAFYRAMAIFASKHLGKSSSIITDIVFKVGIFFRGI
                     ISLVAKFFRRRMVMIVDGIALVAAILVAHQLQPKPLPDYPSLLSMLVFYLLVWLGIGY
                     SIGLYDRRELSYSRAVVASLLSFVASLIFNLIFKDVIYSPNMLLLSFIIVTLFLPGWR
                     VLLLFLQRRRVIQPASSLSKALLSRRTIIVGAGKEGERIARKLKTHIEHGFEILGFVD
                     KDYMNEEIAGFPFLGLVSDLPEIIRINHATEIIFTTDRFNNDDILGIVDKIKKYRTNI
                     KIVPRNLDYILGKSSVENIEDIPLVNIDYNLFHVGNRVTKRIFDLLISSISIILLSPF
                     VIPIAFLLGYRFQKANFIGAEQHQISALTLVKRNGNHVHPFLNRFPLLFSVVKGDISL
                     VGSELVPAENGERNLRFKPGLTSLFRVSKNHRTDENDKQNYEHFYMQNHSLFLDVEII
                     LKTVLKI"
     gene            complement(6954..7670)
                     /locus_tag="PLO58_04205"
     CDS             complement(6954..7670)
                     /locus_tag="PLO58_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HQO74026.1"
                     /translation="MKSLVIIPTYNEKDNIRTVIERLQALPIELDILIIDDNSPDGTA
                     DIVRELQADDPHIFMINRPAKLGLGTAYITGFRWALERDYEYILEMDADLSHNPDDVP
                     RLIQECENGYDLVIGSRYCNGVNVINWPIKRLLLSYGANKYTRMITRMPIMDATAGFK
                     CYRREALAAINLDRVKSSGYSFQIEMHFRVWDAGFKIKEIPIIFIERSEGLSKMSKNI
                     VWEAVFMVWKLKLRQIFRSL"
     gene            complement(7639..8550)
                     /gene="rfbD"
                     /locus_tag="PLO58_04210"
     CDS             complement(7639..8550)
                     /gene="rfbD"
                     /locus_tag="PLO58_04210"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HQO74027.1"
                     /translation="MKILITGANGMLGEKCTRLLATKHRVLATDLHDKLIYKLETDYR
                     KMDITKPEEIENIISEFQPDWVVNCAAYTNVDGAETDRETAWQVNAVGPMNLVAALRK
                     SNTPIIQISTDYIFDGVNGPYRENDPVKPINYYGETKLAGERAVLESGLPATVFRTNV
                     LFGSSTNQEASFVYWVVNKLSHREPINVVNDQFGNPTWADGLAQVIEMAIERGINGLY
                     HYGGADYINRLEFALQIAAVFELDPTLIRAVSTRSLNQKAARPFRSGLICEKIQSEFG
                     IHLYSVKEALTNMKEKQFEVISNHTDI"
     gene            complement(8553..9014)
                     /gene="purE"
                     /locus_tag="PLO58_04215"
     CDS             complement(8553..9014)
                     /gene="purE"
                     /locus_tag="PLO58_04215"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQO74028.1"
                     /translation="MEKIALIVGSNSDLETVEQARKYLEYFELDCEIKVLSAHRQHDE
                     LVNFVKRFEKENGRLIIACAGMAAHLPGIIAALTTLPVIGVPLNASSLNGVDALYSIV
                     QMPGGIPVGTMAIGKPGIINAIIYAARILALSNAKLKAKLVEFAAAGFKLN"
     gene            complement(9090..10109)
                     /locus_tag="PLO58_04220"
     CDS             complement(9090..10109)
                     /locus_tag="PLO58_04220"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HQO74029.1"
                     /translation="MNKKIGLLVLIISGMLMNCNQLRESIGGVDDIYIFATDQTREVL
                     GKAIDTTFSYGIRTPEFQPYFSTKWLPVEEFPSYIKFKNIILVADLTQEDLAAEIARG
                     ILPKQSLIAAEKDSIHMFAIPDNWARDQMFVLIAGRNLKMVERSILEQKGWLYGKFEE
                     KFEEVQMDYIYGRYEQKKLTNYLWQKYRWTMRIPHDYIIIRELPENNFVWLGRGLPYR
                     WISVTWGDGIQTEWLTNVGLYEKRNEIGKLYGGIMTEKRFLGFQFFKFGEYDALKMYG
                     LWYHTEETQGGPFETYAFYDWRTDRTFIIDMLMYGPGEKLSVLFRGLELTAKTFTTDY
                     KGSWK"
     gene            complement(10180..12285)
                     /gene="rnr"
                     /locus_tag="PLO58_04225"
     CDS             complement(10180..12285)
                     /gene="rnr"
                     /locus_tag="PLO58_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQO74030.1"
                     /translation="MKVRTDTQIVERIIQFLQRNKGNIYKQKKIAREVGISSNKYVRF
                     KQILKELAIEGEIENPRSGWYRIPDKARFVEGIISFSGRGFAFVNTDAGEEIFVGADH
                     TANALHHDRVLIEKLRYSNGIRPEGKVVKVLNRGQKSIIGTMKRKNKHWVVIPEHPAA
                     VEEIELVGSEKGLREGQLVEIQDIEWSNARLRPRATLKQILGFPENPQDDLIIVKKMY
                     RLSDRFPNKAWQEANRLGNQKIKADNRLDLRDKFIFTIDPPDAKDYDDAVSLEKSPDG
                     NWLLGVHIADVSHYVTPGSVLDREARRRGTSIYLGENVIPMLPPPISDELCSLKPGED
                     RLAFSVILTLNGAGAVVRYSFAPSIIRSFRRFSYEEVQEILNTKSGEYFDMFTNMRQI
                     SRTLFERRRAAGSIDFDIPEPVFKMGPAGIPIEINPSERLDSHRIIEEFMLAANRAVA
                     EYISIKRRNEKLPFLYRIHPQPPQQNITELYQILRNLGLDLHQPPKLKPMDIQKILNG
                     LAEMPYKNFIEQITLRSMAKAIYSHQPLSHFGLAFPHYTHFTAPIRRYPDLVVHRLLK
                     NYLGDYDSNDLAFYRRSLPHIASSASQREIEAMEAERTYQKIKQVRFMADKIGQWYKG
                     VISGVREFGFFVEISQFLVEGLVHIRTLSDDYYIYDPENHLLRGKYSQRIFRLGDEVM
                     VMVTDVSVRERRIDLEWGE"
     gene            complement(12324..13850)
                     /locus_tag="PLO58_04230"
     CDS             complement(12324..13850)
                     /locus_tag="PLO58_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQO74031.1"
                     /translation="MTDQRHRRKNLQIVIFLIIGFVGFLPLLGIAQDTSRAEVIFGEI
                     TVRALEEKLTDFTEIAKDYTNYPVTADSYEELTNKILDAVLKDGYLYPVLTLSAVQPE
                     TEGNLTKLNPEFRLDRGERVVIDTVIYEGVKRTAPELLKRESAAILGKSPDSQKISAF
                     LNNFKSYSFLTVQSQPEIVKTREGQFGLVVQLTEHSTNTFSGVVGYIPATATTEGYFT
                     GEMNLNLNNISGRGRSLSVFWAKPNRYSQQIDLRYFEPWIRHTNFNAEGQFSQILRDT
                     LVVLRSFEAGIGRRLFQNGSGALKFNYTSTMPTSGGRQLLGLNDLTTRGLGLSFRYDG
                     RDYPLNPQKGIMVAINAFLSRRIEQNVGRLWQYQTEINGELDVKIRSQLVAACGWNLR
                     GKWLSEGVPVYSDYYWFGGSRSLRGYATELFGGSEVAWLNNEIRWLIGKNGRAHIFFD
                     QGYYRRSVEGLDKKYFPHSYGVGLRLDSRMGVIGLDYGFGEGDTFTTAKIHIFLESKF
                     "
     gene            complement(13850..17113)
                     /locus_tag="PLO58_04235"
     CDS             complement(13850..17113)
                     /locus_tag="PLO58_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74032.1"
                     /translation="MDTLKIAASDSVVYLSHRFVIPETIELNVTDSISLDPLTGAITF
                     HSTRTDSAFFIAKYQYLPLNLPLNRIVNLPPRIYTGNEIAEPSSTDYPAHPPAQYSTE
                     TTDFLKSGTLYRGVTLGSQSGMSLQSGLNLELQGKIAEDIAIVGTITDQNIPIEPEGN
                     TQTLDEIDKVFITVALPHERLTFGDYEMAYSSGSLGNYNRKLQGVYLESERPPMRNVM
                     SGAVTKGQYYHNYFLGEESNQGPYQLIGRNGETDIIVLAGTEKVWLDGRLLTRGESND
                     YIIDYSTAEITFMTRQIITAESRISVDFQYSDLIYQKNIYMAGNTTNLFDEKLRVSAA
                     AITEKDDRENPIELVLSGADKKLLKSLGDNAQDAYISTITSDTNGSYILVDSILIYAG
                     PGQGTHTATFYNVGKAGSYKKVYRGEVTYFEWVDKNNPLTVASEIASALYLPVKPLKL
                     PTSRTLYHLASDWRPHQNFAIKAEVARSDFDQNLFSPLDDEDNAGLAVDLTSNLIIPL
                     SESSKIALAGHYKQENWGFEPIDRHQIVEYRRKWDLPSDSTNGEQYYEGQIQYALKNN
                     LVVKTEGGQYSRGDFNSNRFAASAGLSYKMLENVQISEEFIQRNQTGFNDINWTRRGF
                     RLITHLWKVKPFATLNYELREGDTIRTENFRFLEQIYGIGSASPGRLTWQVQTTLRRD
                     DLSDSNTWIKGTNARNIGLNGQLINWHSFSSQWNYIYRIKKFYNQNNSDIVVHLLDFS
                     LKQEPRKLPYRWETTLKIENEQTVKKEWRYYYVGDGMGDYIYDSTYADYVPDPQGDYI
                     LRILPSSIKVPITSIEDGLRFQFDGANVKMPIFGDWLKRISTLTDVRLRQEIHDPQGI
                     IKYLATDINQVDTSWANYSRIIQQDVNLRSRERKSDLRLRFYSSDLITQADVRGLEKT
                     FTNEWSLRYRNRYLGKTTIESESALKNYSRQSEINTLRERDIYTVREKLTISTFLDRI
                     HLIGTEITLNSDKAKGSDPIESLLAGVKLSYERKIVGKGRWKSFVELDKVSVTPKGKA
                     IPYEMSNGKKEGLTGGWGTTVEYRVGSNVSIRVNYEGWKEPERSVYHLGSGEVRLSF"
     gene            complement(17256..18158)
                     /locus_tag="PLO58_04240"
     CDS             complement(17256..18158)
                     /locus_tag="PLO58_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74033.1"
                     /translation="MKRSSKISLLIFILIWNCTIKAQFRFEREIVPQSVREGVQFNAI
                     SVNEFDDIYLLESKFSEVYRLDQNGNILNRNGGFGWGLGQFDCPRDLCFSGLDVIVAD
                     QNNHRLVRYDRQLNYIATQDLRSDSRRLIYPMSLTASQINELFILSGETAEILRLYVE
                     KNEQTWFGGIEYGVYALVHPVSLRINRKGILTVLQEDGSLVQFDRFGTPLGLIPFHNS
                     KINKCKAYGLTAVNNDWLILIDQTPFLRMFYSKSKIWDEPTLADFDRLEPFIATTFSN
                     NRLYLLTSNCTILVFSMETSQSKP"
     gene            complement(18442..18906)
                     /gene="tnpA"
                     /locus_tag="PLO58_04245"
     CDS             complement(18442..18906)
                     /gene="tnpA"
                     /locus_tag="PLO58_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HQO74034.1"
                     /translation="MKSGVYTQIYLQLVFAVKNRDAVLNSDMLKRVFEYMSGINTKLG
                     HKSIIVNGHYDHAHLFIGLNPNISISDTMHDIKRNTSIFINKNKLCYGHFNWQEGYGA
                     FSYGRSQINDVFRYIQNQEAHHSKRTFREEYIEFLKEYGIGFDSQYLFDFFD"
     gene            complement(19205..20437)
                     /locus_tag="PLO58_04250"
     CDS             complement(19205..20437)
                     /locus_tag="PLO58_04250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQO74035.1"
                     /translation="MKRFILIILVVSVLIVTVFGEESVVIKRENTHFRQGPGSYYPLI
                     GMLQKGVQVAVVGTQSGWMNIQCAGQIGWISENAVIASDEPTGSILTGSISGNGSMMI
                     SRASASGAVKGFAQKFVNFHDGDPAFIEQYDVELFTPAEFQRFRQETFRGRDPEKIRK
                     RYKRIKSENTDHSISLQDEKIGLAAASRIAASGLVANQSQLKYINLVGNIVLENTDMY
                     DYPFKFYILKDSHPSAYAIPNGMIFVTSGLLELLQDEAELAVILAHEISHVVQKHGAE
                     EIAKRKTMIVADEGFNELDQEIVTEDATADELDLIALNCYETATKRRQIEYEIEADQL
                     GMIYAYRSGYDPSALWRVLTRIEANTSPDFWNPESNWSYDAVFDRAERVNTFIKKNLN
                     KNPEWNVTNPSRFRSVFK"
     gene            complement(20506..22665)
                     /locus_tag="PLO58_04255"
     CDS             complement(20506..22665)
                     /locus_tag="PLO58_04255"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQO74036.1"
                     /translation="MKINTKKILIGLGIALIITLLSGLLYHNGLIQRWENQVNDILYR
                     DFQVEQHPSEAVIIAIDQNSLDYFQKNFQILWPWPRDIYAMATNYLNSCGAKVIVYDI
                     IFSTPDIDRLNVQAEYADSAFARAMLSSGKVILAMQMEDSTHLSVEKLPTQFNRQIVY
                     NVPQRIVYKYPNVTAPIERFQKAMAYPGAVNFFTDNDGVCRRVPLFFAKDHQIVPYMA
                     LAAVMIYDDISSIDYDSMRHTIFAGKRRVPLNNSGFFDIYWYGPGGAGNTFQYVSFAQ
                     LLNSYLQVQNGQKPDLDPAIFQDKAVFIGATAAGLLDLKTTPFSSVEPYPGVEIYATI
                     FSNIIRGDYISNLPFIIWLGFSLILIWLLAFLWQKLKIWQSAGISLIILFIPLIMSVM
                     IFQRLKMFMPVVTTEIAFVLSITVVLLVNYLTEGREKKMIKKVFNRYLHPTVVEVLTR
                     NPERLEMGGKEIVATVMFSDLQNFTGIAELFTPPEIVGFLNQYFTKVEQIIFKNDGML
                     DKYTGDGIMAIFGAPIPTNEHARMACNAALDFKNLSTFTIEVKNRTIPLITRLGINSG
                     NFVVGNIGSANRMDYTAIGDTVNLAARLEGVNKIYGTQNIISETTYSLVKENFICREL
                     DLIRVKGREEPLAIYNIIGIINDLSSELEKMLKLHNEALQQYRERKFKTAELAFLELN
                     RQFPEDSVAKVFAKRCVQLINEPRLIDEDGVFNITVK"
     gene            complement(22687..24132)
                     /locus_tag="PLO58_04260"
     CDS             complement(22687..24132)
                     /locus_tag="PLO58_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HQO74037.1"
                     /translation="MIKNIHRIIGLLCLIIWIDCEGSDCRALIENGEFKKAEEIIRRE
                     LFSNPSLSVSERRTLEFELERMKRIRLDFNKSQAEVIEYIKQYVPDVTPTDLVRWESE
                     KSLEYLVIDGEKMYFKDAAPNLFRLDRTLKAVKQKVDSQIPTDSRREPAFDLDSHCRK
                     IVQAVQTTQKSTVLPVRMKIKQSLTVHPNVVPEGKIIRCWIPFPREIPDRQVNIRLLK
                     TDPPIYILANTDQTLQRTIYFEKAAVKDSTTHFSVEYTYEIYGSYTPVKAEKVIPVEP
                     NPDLNPFLSEEPPHIVFTDEIRQLSQRIVGSETNPYRIAQKLFEWIDVNIVWASAREY
                     STIYNIPQYVINNRHGDCGMMVLTFITLCRLNGIPARWQSGWEFKPPQDSMHDWGEIY
                     LAPYGWVPMDVTYGRRATADESFRWFYLSGMDSYRLIFNDGMAADFYPLKIYPRSETI
                     DSQRGEVEWEGGNLYFNQWNWEHDWEIISEP"
BASE COUNT         6964 a         5570 c         4642 g         7039 t
ORIGIN      
        1 ccaatgattc gccgtacata gcgcaatttc ttcagcaaat aatcattata attagccgtg
       61 ccgggtttga gactattaat ccttacccaa gccgaacaat gaatatactc atagtcccca
      121 atataaatgg caacgtgatt aatttgctcc ggatttggac cgaaaaacaa caaatcgccg
      181 ggcattaaat tctccagtaa attggtcgtg tcaaccgcaa atccctgatt gacctgcatg
      241 ttggcatccc tggctaatag ataaccgttg cgacgataaa ccgtttgggt aaaaccactg
      301 caatcaaaag ctttgggcga attggcaccc cagagataag gcctcccaag taaattcttg
      361 gcagtcgtta taatcccctc cgcggtcgga gttcttgcca gatattccga ttcgctaacc
      421 aactgctggg acggaataaa accccggcga ccgtcaggaa tttcgacgcg agtccattgc
      481 ccttgcttgc caatcatttt aaatctattc ccgaggacaa catcggctac cggcatagat
      541 tttcggtcag ccttagagta aatgatgccc tcgaaatcat cgtaaacgac tttcgatgca
      601 ttttcccagg cggtctttag tgagtcgccg ccaactacta atcggtcatc ctccatccat
      661 cccaggtaac catcatcggt cttcaccaaa aaacagcctt cggcactttt caatatttcg
      721 accgtcaatc cccgtaaggt ttgcgttacc atatcgttaa ataccgatgg tccactccgc
      781 aaaggctcaa ccgaattaat cacaatgcca aaattctttc cgttcaccgt ttcatcgggc
      841 aatattttaa taagattttg aattttaata gctggcaaag tttgttttat ttgattagtt
      901 aaagcccgga ctgcttcggg atggtcggtt tccccgacta aattataatc attatcctga
      961 taatcaattt caattttcca tagcgcgact cgttgatccg gacaatattt cttgcgaatt
     1021 tctgcaatga tcgtttcaat ttgttgcttg acttctgttg tatctgcctg gcaaatttta
     1081 ccaatactaa aaatgaagag cattaaggca aacagttgaa tggtagttcg caaaaaaacc
     1141 tttacctggc taaaccaagt gtcgttttat attttccgtc atccctgacc gctaaaattg
     1201 cctcggaaac gaccttgtca tactttaaat cggctttaag ttgtccggcg attccatccc
     1261 tgagcattgc gaactgattc tcaattccac gctggatcat caggtgatta ttttcgaatt
     1321 cggcttcttt taatgaatca aatatttcat ataccggctg gaaatcaatt ttgacgttct
     1381 ttaagcgttc atcctggcta attatcttat ccatctcacg gatgttcttt tcggtttttt
     1441 cgataaattg aaatcccgaa tgatcaatat aatttttgaa ttctttgatg atccgttcgt
     1501 taacctcttc tggtaattca ttatgttcag ttttatactt aacggcaaat gaataaaact
     1561 gattttgacg ccagagttcg gttataaaat cgctctgctc atcgctttta ataataatat
     1621 ccggggtaat tccacccttg ctttttagct gacgcttatg gcgtgaataa aagatcgaat
     1681 cctgagacgg actggcggcg attaactttg gatttttgat ataatcaggc ttttgaatta
     1741 atcgcccact gggaagataa tatttggccg tggttatctt gagagaatgc tctttatcaa
     1801 cgcgaaaaac ggtctgtact aaacctttac caaaactggg agtacccaga acgattccgc
     1861 ggtcgagatc ttgaattata ccggcaacaa tttcactagc ggaggcactg ccgccgtcaa
     1921 ttaaaacggc tatttttacg gattcatcca agagtggtcg gcgacgggca acatcaactc
     1981 gattggagct ctcattttga ccacgcgtaa ataacaaagt gtctcccggc tgagtaaaca
     2041 actcggctac cgttagtgct tccgttaata atccccccgg attgccgcgt aaatctaaga
     2101 taagggcttc aagctggtca tgatctttaa aattttcaat cgcctggcgt acttccgccg
     2161 ttgacccttt ggaaaattca gccaatcgaa tataaccgat gccttcttct aaataatcgg
     2221 cataataaac atcataaacc tgaatttttg cccgctctaa aatatatact tttccttcat
     2281 cagcccccgg acgccgaatc actaattcga ccttggtacc gacattgccg cgaatcaggc
     2341 gggtgcattt atcgaggtcg agaccggttg tccagattgt atcaattttt aaaatttgat
     2401 caccaggcaa aatgttcgct tttttagcgg gactgccttc gatcggtgaa attacagtca
     2461 gcgtgtcatt gcgcagacta atccgtaagc cgactccgcc atattctcct ttcgataaaa
     2521 tttccagcgg ctctttttcc tcttcggtca tataaactgt atagggatcg agctcacggg
     2581 tcatattccg aatattactt tcggttaaag cggcggcatc gagtggatcg gcgtaattca
     2641 gcaatatttg ctgaaagact gcattataca gcttgtaaga atccgaaatc gttttataaa
     2701 tatccttcgc cgtaaaagcc aataagacac ttacggaaac cagcacggca atccagattt
     2761 ttaaaccttt tttaaatgat aatttcatat ttgcatcttt ctaataattt tattgaattt
     2821 atgccaaact tcatcagcca ccaactcttc cgttttagtg ccatcaatta aacaaacacg
     2881 attgggttca gcggcggcga ttttcaaaaa tccctgtcgg attttttcct gaaaattatg
     2941 ttcttccctt tcgagacgat caagatcacg cgtaccaagg cgctggagcc tttccgcaac
     3001 cggaatatcg aagataaaag tcaaatcggg taccagatta tcagcggcaa tgcgattgag
     3061 catatttata aaatttaggt ctattcccct accatagccc tgataagcag tcgtcgaatc
     3121 gtaaaaccga tcgcagacaa cgatttttcc cgattccaaa gccgggcgaa tcaattcccg
     3181 acaaagctgg tgtcggctgg ctgagtaaag taaaagttca gtaatggcaa acatttctga
     3241 gttggattta cttaaaagaa tttcgcggat tttttcaccg atcggcgtac cgcccggttc
     3301 ccgaaataac gccactggaa tgttttctcg ctcacacttt tttatgaaat atttaatctg
     3361 ggtcgtcttg cccgaaccgt caatcccttc gaatgtgatt agcagacctt tagtcattaa
     3421 tgaatacttt tctattgaat tttactaata tactagaaac tctattttga catttgcgcg
     3481 gctaataatt ttaggaaaaa ttggggcgga cggactgctt ttccggattg attaacaaag
     3541 gcgtgaatcg tatagccgct aacgagaggc tgagattcat tttcacgtag aatttcgtaa
     3601 tcaattcgaa gccgggctgt tggcattgcg gaaatgcgag aaataattgt tagcaaatca
     3661 tcaaaatgcg cacttcgatg ataaacgcaa tgggcttcca ctaccggcat cgcataacca
     3721 gcctgttcaa attgactata cgggagtcca atggctcgca ttatctcatc gcgggcggct
     3781 tcgaagtatt ccagatagcg tgaataataa acaactccca attgatcaac actcgagtaa
     3841 cgaactcgca actggtaatg gtgttctaac atattaaaag cctatcaaat tttttatttg
     3901 gatttggatt taacgtcaac cactttccac gaacccatac gggcatattt ttcaattcgc
     3961 tggtcaacta attcatctat cggaatctgc gttaattcag ccaaatacct gagcaagaca
     4021 tctttcagag tctgcgccat ctgatcggga ttacgatggg ctccgccaag tggctccgga
     4081 ataatttcat cggcgatttt aaattcggca agatcacggg cggttacctt cattgcctcg
     4141 gcggctaacg gcgccttgga gctatctcga tataagatcg aagcacaacc ttccggagta
     4201 atgaccgaat accaggtatt ttccagtaaa aggatgcgat cgcctacccc gatgccgagc
     4261 gcgccaccgc tggcaccttc gccgattact accacaatta tcggagtgcg caagcgagcc
     4321 atctcaaata agttacgggc aatcgcttct gcctgtccgc gctcttctgc accaactccc
     4381 ggaaacgctc cttgcgtatc aatgaaggaa attaccggac gtttaaattt ttcggcaagt
     4441 ttcatagccc ggcgggcttt gcgataccct tctggatgcg gcataccaaa attccggtaa
     4501 aggttttcct tagtggttcg ccctttctgc tgaccaatca aaatgacttt ataatcttca
     4561 atttgcgcca atccggctac aacggcttta tcatcggcgt aatatcgatc gccatgaatt
     4621 tctatgaatt cggaacaaat tcttttgaca taatccaggg tatatggacg ctctggatga
     4681 cgggcaagtt gaactctctg ccagcgagtt aaattagagt aggtattttg aatcaggcgg
     4741 ttgagtttga gcttggcttt ttcaatttcc ggattgaggt ctaccccgct ggccgcactt
     4801 tgtgctcgca gttcctctat ttttttctcc aattcaatta ttggtttttc aaaatcaaga
     4861 actatttcag ccattatttt ttcaaatttt taaaaccgtt ttcaaaataa tctcaacatc
     4921 cagaaacaaa gaatggtttt gcatataaaa atgttcgtaa ttctgcttgt cgttttcgtc
     4981 ggtgcgatga tttttactta cccggaaaag actggtcagt cccggtttaa aacgcaaatt
     5041 ccgttcccca ttttcggctg gtaccaactc actcccaact aaactgatat cgccttttac
     5101 aactgaaaac agtaatggga aacgatttag gaatggatgg acatgattac catttctttt
     5161 taccaaagtc agcgcactga tctgatgttg ttcagccccg ataaaattgg ctttctggaa
     5221 tctataaccc agcaaaaacg ctatcggaat aacgaacgga gatagcaaaa ttatacttat
     5281 tgaagaaatt aacaggtcaa aaatccgctt ggtaacccga ttgccgacat gaaacaagtt
     5341 atagtcgata tttaccagtg gtatgtcttc aatattttca acactcgatt tccctaaaat
     5401 gtaatccaga ttgcgcggaa cgattttaat attagttctg tactttttga ttttatctac
     5461 aatccccaga atatcatcgt tattgaagcg atcggtagta aagataattt cagtagcatg
     5521 attaatccgg atgatttcag gtaaatcgct taccaatccc agaaaaggaa atccggcgat
     5581 ttcctcattc atataatctt tatcaacaaa acccaaaatt tcaaagccat gttcgatatg
     5641 agtttttaac ttacgggcaa ttcgttcacc ttctttaccg gctccgacga taatagttcg
     5701 ccgggacagc agagcttttg acaatgatga tgccggttga attacacgac gtcgctgtaa
     5761 aaataataat aacacccgcc agcctggtaa aaacagagtg acaatgataa aactcaataa
     5821 cagcatattc ggcgaataaa tgacgtcctt aaaaatcaga ttaaagatca gagaagcaac
     5881 gaaactaagc aaggaagcaa caactgctcg tgaataggat aattcccgac gatcatataa
     5941 gccaatagaa tatcctattc ccaaccaaac cagtaaataa aaaaccaaca ttgatagtag
     6001 cgaaggataa tcagggagag gcttgggttg taattgatga gcgaccaaaa tggccgctac
     6061 caaagctatc ccgtctacaa tcattaccat tcggcgtcgg aaaaatttcg ctaccagtga
     6121 tattatcccg cggaaaaaaa tgccgacttt gaaaacaata tcagtgatta tcgaggaact
     6181 ttttcccaga tgtttgctcg cgaaaatagc cattgctcgg taaaatgcga taaaattatc
     6241 gaaggcggca tgtttggcac tttcgccttt ataatgaata attttcgtat ccggcacata
     6301 ataaattttc cagccgcttt ttttgattcg atagcagtag tctaaatcct caccataaag
     6361 aaaataggat tcatccaatc cgccaatctg ttcatatacg gctctcctca tcattaaaaa
     6421 tgaaccgcta accgcatcaa cttcggtgat ttgatcggga tccagatagg taagattata
     6481 acgcgaaaaa gccttcactt ttggaaatag acggctcaac ccaagtaatt tcggtaacgc
     6541 cactgtcaaa gttggaaaag agcggcgaca agccagttgg agtgaaccgt cagcgttcag
     6601 tattttacat ccggcggctc cagcctccgg atgagcccta aaaaattcga tcattacttt
     6661 aattgtatct tcctgaagca tcgtatccgg atttaataac aaaatatatt cgccacgcgc
     6721 gattgccaat ccttgattgc atgccgccgc aaatcctttg ttaaaatggt tttctatcaa
     6781 tatgatttcg gggaatttat tttttatgac attaacagta ccgtcaaccg aatcattatc
     6841 aactacgatg atttcacttg ataaaccctc aagggcattc ttcaaagaca aaagacactg
     6901 ttgaagaaat tctttaacgt tgtagctaac aatgatgacc gagatttgca tgtttacaaa
     6961 gatctgaaaa tctgccgtaa tttcaatttc cagaccataa atacagcttc ccaaacaata
     7021 ttttttgaca ttttagacaa gccttcggaa cgctcgataa aaattatcgg aatttccttg
     7081 attttaaaac ccgcatccca aacccggaag tgcatttcaa tttgaaagga atagccggag
     7141 gattttacgc gatctaaatt aatcgcggct aaggcttccc gccggtaaca tttaaaaccc
     7201 gcggttgcat ccataatcgg cattcgagta atcatacgag tatatttatt agccccataa
     7261 ctcaggagca aacgcttgat aggccaatta atgacattca ctccattaca atagcgcgat
     7321 ccgattacca gatcataacc gttctcgcat tcttgaatga gtctgggcac atcgtccgga
     7381 ttatgcgaca aatcagcatc catctccaaa atatattcat aatcacgttc aagtgcccag
     7441 cggaaaccag tgatataagc cgtccctaaa ccgagtttag cgggtctatt tatcataaaa
     7501 atatgaggat catcggcttg taattccctt acaatatcgg cggttccatc aggactatta
     7561 tcgtcaataa ttaaaatatc cagctcgatc ggtaaagcct gtaaacgttc gataacggtg
     7621 cgaatattgt ctttttcgtt atatgtcggt atgattacta atgacttcaa attgtttttc
     7681 tttcatattt gttagagctt ctttaaccga atataaatga atcccaaatt cagattgaat
     7741 tttttcacat attaatcccg atcgaaaagg tcgggcggct ttctgattca gcgaacgagt
     7801 agaaacagcc cgaatcaaag tcggatccag ttcaaaaacc gccgcaattt gcaaagcaaa
     7861 ttccagccta ttgatataat cagccccgcc atagtgatat agcccattaa ttccccgctc
     7921 aatcgccatt tcaatcactt gcgccaagcc gtcagcccag gtaggattgc caaactgatc
     7981 attgaccaca tttatcggct cccggtgact gagtttattc accacccaat aaacaaagct
     8041 ggcttcctga ttagtcgagc taccaaaaag gacatttgtc cggaaaactg ttgctggtaa
     8101 ccctgattct aaaacagcgc gttcaccggc taatttggtc tcgccataat aattgatcgg
     8161 ttttaccgga tcattttctc tatacggtcc attgacgcca tcaaaaatat aatcggttga
     8221 aatctgaata atcggagtat tagattttcg taaagcggct acaagattca ttggaccgac
     8281 tgcattaact tgccaggcgg tttcgcggtc ggtttcagcg ccatctacat ttgtataagc
     8341 cgcacaattg acaacccaat ccggttgaaa ttcactgata atattttcga tttcttccgg
     8401 ctttgtgata tccattttgc ggtagtcagt ttctaatttg taaatcagtt tatcatggag
     8461 gtcagtcgct aaaactctat gtttcgttgc tagcaaacga gtgcattttt cacctaacat
     8521 tccgttagcg ccggttatta atattttcat tattagttga gtttgaagcc tgccgccgcg
     8581 aattcgacca gtttagcttt taatttggca ttggagagcg ccaaaattcg agccgcatag
     8641 ataatggcat taatgatccc aggtttgccg atcgccatcg tgcccaccgg aataccgccc
     8701 ggcatttgca caatggaata gagggcatct accccgttca aagacgaagc attcaaaggg
     8761 acgccaatta ccggcaaagt agtcagagcc gctataatac ccggcaaatg cgccgccatc
     8821 ccagcacagg cgataatcaa acggccgttt tctttctcaa accgctttac aaaatttacc
     8881 aattcgtcgt gctggcgatg agccgaaagg acttttattt cgcagtctaa ttcaaaatat
     8941 tccagatact tgcgcgcttg ttcgactgtt tctaaatcag aattgcttcc gacaattaat
     9001 gctattttct ccataaatct acttgctccg ccgtaattta attgaccacc ctataattgc
     9061 caaattccga ctaccgacca gttctagctc tatttccagg agcctttgta atcggtggta
     9121 aatgtcttag cggtaagttc gagcccgcga aataggactg acaatttttc accaggacca
     9181 tacattagca tgtcaataat aaaggttcgg tcggtgcgcc agtcataaaa ggcatacgtt
     9241 tcaaatggac caccttgcgt ttcctcagta tgataccaga gtccatacat tttcagagca
     9301 tcatattcgc cgaatttgaa aaactgaaat ccgaggaagc gtttttcggt cattatcccg
     9361 ccatataatt tgccaatctc attgcgtttt tcgtataatc ccacattcgt taaccattcc
     9421 gtttgaatgc catctcccca ggtaaccgaa atccaacggt acggcaaacc tcttccgagc
     9481 caaacgaaat tattttcggg caattctctg ataatgatat aatcgtgcgg aatacgcatc
     9541 gtccaacgat atttctgcca gagataatta gttagttttt tttgctcata gcgtccatat
     9601 atataatcca tctggacttc ttcgaatttc tcttcaaact ttccataaag ccagcctttt
     9661 tgttccaaga tactgcgttc aaccatcttc aaattgcggc cggcaattaa aacgaacatc
     9721 tgatctcgag cccagttatc cggaatggca aacatatgaa tgctatcctt ttccgcggct
     9781 attaaacttt gctttggtaa tataccacgg gcaatttcag ccgccaggtc ttcttgagtt
     9841 aaatcggcta ccaaaatgat gtttttaaat ttgatatatg atggaaattc ttctaccggc
     9901 aaccattttg ttgaaaaata gggttgaaat tccggcgtcc ttattccgta ggaaaaagtc
     9961 gtatcaattg ccttacccag cacttctcga gtttgatcgg tagcaaaaat ataaatatcg
    10021 tcaacgccgc cgatagattc ccgcagctgg ttgcaattca tcaacatccc ggaaataatc
    10081 aaaactaata atccaatttt tttattcatt tttatacccc atcaattatt caactttaaa
    10141 attaaacatt cattggtatt aagccaataa atcttttaat cattcacccc actccaaatc
    10201 tatccgtcgt tctctaactg aaacatccgt caccattacc attacttcat cgccaagccg
    10261 aaaaatgcgt tgactatatt taccacgtag aagatgattt tccggatcgt aaatataata
    10321 atcatcactc agagtccgaa tgtgtaccaa tccctcgacc agaaattggg aaatttccac
    10381 aaaaaatccg aattcacgca cgccactaat gacgcctttg taccattgac cgatcttgtc
    10441 cgccataaaa cgcacctgct tgattttctg gtaagtccgc tcggcttcca tcgcctcgat
    10501 ttcacgttgg ctggcagaac tggcaatgtg cggtaatgaa cgccggtaaa aagccaggtc
    10561 gttagaatca taatcaccta aatagttttt cagcaaacga tgtaccacca gatcgggata
    10621 acgtcttata ggcgcggtga aatgggtata atgaggaaac gccaaaccga agtgacttaa
    10681 cggttgatga gaatagattg ctttagccat cgaacgcagt gtaatttgtt caataaaatt
    10741 tttatagggc atttcagcca atccatttag aattttctga atatccatcg gtttgagctt
    10801 gggaggctgg tgcaaatcga gccccaaatt tctcaaaatc tgatacaatt cagtgatatt
    10861 ctgttgcggc ggttggggat gaattcgata gagaaaaggc agtttttcat tgcgccgttt
    10921 aatagatata tattcagcga ctgcccgatt agccgccagc ataaattcct ctattattcg
    10981 gtgactgtcc aatctttcag aaggattgat ttcaatcgga atcccagccg gtcccatctt
    11041 aaaaactggc tccggaatgt cgaagtctat actgcccgcc gcccgtcggc gctcaaaaag
    11101 agtgcgacta atctgacgca tattggtaaa catatcaaaa tattcaccgc ttttggtatt
    11161 cagaatctcc tgcacttctt cgtagctaaa tcgcctaaac gagcgaatta tactcggagc
    11221 aaatgaatac cgcaccaccg cacctgcacc gttaagtgtc aagataaccg aaaatgccaa
    11281 ccgatcttct cccggtttca gactacacaa ctcatcagaa attggcggcg gcaacattgg
    11341 aataacattt tcgcccaggt aaatactggt accccgccgc cgagcctcgc gatcgagtac
    11401 actgcccggc gtcacataat gactaacatc tgctatatgc acgcccagaa gccaattacc
    11461 atctggactt ttctccagcg atacagcatc atcatagtct tttgcgtcag gaggatcgat
    11521 cgtaaaaata aacttatcac gcaaatcgag acgattgtct gcttttattt tctgatttcc
    11581 gaggcggttg gcttcctgcc aagctttatt cggaaatcgg tcgctcagcc gatacatttt
    11641 ttttacgatg atcaaatcat cctgcggatt ctccggaaag ccaagaattt gttttaaagt
    11701 ggctcgcggt cgcaggcgag cgttgctcca ctcgatgtcc tggatttcga ctaattgacc
    11761 ttcccgcaga cctttttccg aacctactaa ttcgatctcc tcaacagcgg ccggatgttc
    11821 cggaatcacg acccagtgct tatttttccg cttcattgtt cctataattg atttttgacc
    11881 acgattcaaa acttttacca ctttaccttc cggccgaatt ccattgctat accgcaattt
    11941 ttcgattagc acccggtcat ggtgtagcgc attagcggta tgatcagcgc cgacgaagat
    12001 ttcttcgccc gcgtcagtat ttacaaaagc aaaaccgcgt ccggagaacg aaattattcc
    12061 ctccacaaac cgcgctttat cgggaattcg ataccaaccg gaccgcggat tttcaatttc
    12121 tccttctatt gctaattctt tcaatatttg tttgaatcta acatacttat tggaagagat
    12181 gccgacttct ctagcaattt ttttctgttt ataaatattg cctttatttc tttggagaaa
    12241 ttgaataatc cgctcgacaa tttgcgtatc cgttctaact ttcactgact tcggttttga
    12301 actgttattc ttgtgttttc ccattaaaat ttactctcca aaaatatatg aattttggcg
    12361 gttgtaaaag tatcgccttc tccgaatccg taatcgagcc cgataactcc catccgcgaa
    12421 tccaaacgca agccgacgcc ataggaatga ggaaaatact ttttatctaa gccttcaacc
    12481 gaacggcgat aatatccctg atcgaagaat atatgcgccc gaccgttttt accgataagc
    12541 caacgaatct cattattgag ccaggcaact tccgagccac caaacagctc agtagcatag
    12601 ccacgcaaac tgcgggaacc gccaaaccag tagtagtcgg aataaaccgg cacgccttcc
    12661 gatagccact tccctctgag attccagcca caggcggcta ctaattggga ccgaattttc
    12721 acatccaact caccatttat ttcagtctga tattgccaga ggcgaccgac attctgttcg
    12781 atacgacgac tgagaaaggc attaatcgca accattatcc ccttttgggg attaagtgga
    12841 taatcgcgac catcatagcg gaatgataat cccaaaccgc gagtcgtgag atcatttaat
    12901 ccgagtaact ggcgaccgcc tgaggtcggc attgtagatg tgtaattgaa tttcaaagcc
    12961 ccagagccgt tctggaataa tctgcggccg ataccggctt caaaagaacg taaaaccacc
    13021 agagtatctc gcaatatctg actgaattgg ccttccgcat taaaattggt gtgccgaatc
    13081 cacggctcga aatatctcag gtcaatttgt tgagaatagc ggttaggttt cgcccaaaat
    13141 acgctcagac tgcgaccgcg cccgctgata ttattcaaat taagattcat ctcgccggta
    13201 aaataacctt cggtcgtagc agtggctggt atgtagccga ctactccact gaaggtattg
    13261 gtcgaatgtt cagtcagttg aacaactaag ccaaattgac cttcgcgggt ttttactatt
    13321 tccggttgag attgcaccgt cagaaatgaa taactcttaa aattattcag aaaggctgag
    13381 attttttgcg aatcgggtga tttaccaagg attgccgccg attcgcgctt taataattca
    13441 ggagcggtgc gtttgactcc ctcatatatc accgtatcaa tgacaacccg ctcaccgcga
    13501 tccaatcgaa attctgggtt cagtttggtc agatttcctt cagtttcagg ctgaaccgca
    13561 cttaaagtca atactgggta aaggtagcca tcctttagca cggcatccag tattttattc
    13621 gtcaactcct catacgaatc agcggtaacc ggataattag tataatcctt ggcaatttca
    13681 gtaaaatcag tcaacttttc ctcaagcgcc ctaaccgtaa tttctccgaa aatcacttca
    13741 gctcgtgaag tgtcctgagc aattcccaaa agcggtagaa aaccgacaaa tccgattatc
    13801 aagaagatga caatctgtaa attttttcgc ctatgccgct ggtctgtcat taaaacgaaa
    13861 gcctcacttc gccacttcct aaatgatata cgcttcgctc gggctctttc caaccttcgt
    13921 aattaacccg aatactaaca ttcgaaccga cgcggtactc gacagttgtc ccccaaccac
    13981 cggtaagtcc ttccttcttg ccattgctca tctcgtacgg aattgctttc cctttggggg
    14041 ttacagatac tttatccagt tcaacaaatg atttccagcg acctttcccg acaatttttc
    14101 gttcataact caattttaca ccagctagga gcgactcaat tgggtcactt cctttcgctt
    14161 tgtcactgtt aagcgttatt tctgtaccga ttagatgaat ccgatccaga aaagttgata
    14221 tggttaactt ttcccgaaca gtataaatat cacgttccct aagagtattt atctccgatt
    14281 gacgactata attttttagt gccgattcgg actcgattgt ggtttttcct aaataacgat
    14341 tgcgataacg cagagaccat tcgttggtga atgttttttc taatccccga acatcagctt
    14401 gagttatcaa atcggaggaa taaaaccgaa ggcgcaaatc gctttttctt tcccgcgaac
    14461 gcagattaac atcttgttga attatgcgtg aataatttgc ccagctggta tcaacctgat
    14521 taatatccgt cgcaaggtat tttattattc cctgcggatc gtggatttcc tgccggagac
    14581 ggacatcagt caaggtagaa atacgtttta gccaatcacc gaaaatcggc attttaacat
    14641 tggcaccgtc aaattgaaaa cgcaatccat cctcgatact ggtgatcggc actttgatcg
    14701 aagaaggaag aatccgtaaa atgtagtcgc cttgcggatc gggaacgtaa tcggcataag
    14761 tcgaatcata aatgtaatca cccattccat cgccgacata atagtagcgc cattcctttt
    14821 tgacagtctg ctcgttttca attttcagag tggtttccca gcgataaggt aatttgcgtg
    14881 gttcttgctt tagggaaaaa tcgaggagat gaacaacaat atctgagtta ttctgattat
    14941 agaatttctt tatacggtag atataattcc actgggatga gaatgaatgc cagtttatca
    15001 attgaccgtt cagaccgata ttgcgagcgt ttgtcccttt tatccaggta tttgaatctg
    15061 aaaggtcatc ccgacggaga gtcgtctgaa cttgccaagt tagacgtcct ggactcgctg
    15121 agccaatccc atagatttgt tccagaaagc ggaaattttc agttctaata gtgtcacctt
    15181 ctcgtaattc ataatttagt gtagcaaacg gtttgacttt ccataaatgg gttattaacc
    15241 tgaatcctcg acgcgtccaa ttaatatcat taaagccggt ttggttacgt tgaatgaatt
    15301 cttcgctgat ttggacattt tccagcattt tgtagctcaa accggcgctg gccgcaaatc
    15361 tattggaatt aaaatcacca cgcgaatact gaccgccttc cgttttgacc actaaattat
    15421 ttttcaaagc gtattgaatt tgcccttcat aatactgttc accattggtc gaatcggaag
    15481 gcaagtccca tttccttcga tactcaacta tttgatgacg atcaatcggc tcgaaacccc
    15541 aattttcctg tttatagtga ccagcgagag caatcttact ggattcggaa agcggaatga
    15601 ttagatttga cgtaagatca accgctaatc cggcgttatc ctcatcatcc agaggtgaaa
    15661 ataggttttg atcgaaatct gaacgtgcca cttcagcctt tatcgcaaag ttctgatgcg
    15721 gacgccagtc acttgccaaa tgataaagcg tccgactggt cggcaatttt aacggtttga
    15781 ccggcaaata aagcgctgag gcaatctcgg aagctaccgt caagggatta tttttgtcaa
    15841 cccattcaaa ataagtcact tctccgcggt aaactttctt ataactgcca gcctttccca
    15901 cattataaaa cgtcgctgta tgagttccct gaccgggacc ggcataaatc aagattgaat
    15961 ccactaaaat ataagagccg tttgtatcgc tcgtaatggt acttatgtaa gcgtcttgag
    16021 cgttgtcacc gagcgatttc agcaattttt tatcggctcc gctaagcact aattcaatag
    16081 gattttcgcg gtcatctttt tcggtgatcg cggcggcgga aacccttaat ttctcatcaa
    16141 ataagttcgt agtattgcca gccatataaa tatttttctg gtaaatcaaa tcggaatatt
    16201 gaaaatcaac actaatgcgc gattccgctg taataatttg acgagtcata aaagttatct
    16261 cggcggtcga ataatcaatg atataatcat tgctttcgcc gcgggttaaa aggcgaccgt
    16321 ccagccagac cttctccgtt ccagccagaa cgattatatc ggtttcgccg tttctgccga
    16381 tcagctgata cggaccttga ttgctctcct ctccgagaaa ataattatga taatattgac
    16441 ctttggtaac tgctccactc ataacattgc gcatcggcgg ccgctcgctt tccagataaa
    16501 cgccctgcaa tttgcgattg taatttccca aactgccgga gctataagcc atttcataat
    16561 caccgaaagt caggcgttca tgcggcaagg cgacggtaat gaacacttta tcaatctcat
    16621 ccagggtttg cgtgttgcct tccggttcaa tcggaatatt ctggtcggta attgtgccga
    16681 cgatcgcaat atcttccgca attttccctt gcaactccag gttcaagccg gactgaagcg
    16741 acattccact ctgactacca agggtcacac cgcggtaaag cgtaccggac ttcaggaaat
    16801 cggtggtctc ggtagaatac tgagcgggcg gatgcgccgg ataatcagtt gaactgggtt
    16861 cggcaatttc attacctgta taaatgcgcg gcggcaggtt aactattcta tttagcggca
    16921 aattgagcgg caaatattga tatttggcaa taaaaaacgc tgaatcagtt cttgtagaat
    16981 ggaaagtaat agcaccggtc aaaggatcga gactgattga atcagtaacg ttcaattcaa
    17041 ttgtttcagg tatgacaaat ctatgagata aatagacaac cgaatcgctc gccgcaatct
    17101 tcaaagtatc cacctgccac tgctgagcat taagaagcct cgtaaatata aataaaattc
    17161 ctaccagacg agagataatc gtccatcgtt tatatgcttt tcgatataat gaactggcgt
    17221 gattcaaaag ttatatttta accaccagtt aatcactaag gtttcgactg agaagtttcc
    17281 atcgaaaaaa ctaaaattgt acaattactg gtcaacaaat aaagccgatt attggaaaaa
    17341 gtcgtggcta taaaaggctc cagacgatca aaatcagcca aagtcggctc gtcccagatt
    17401 ttcgatttcg aataaaacat tcgcaaaaac ggagtctggt caatcagaat cagccagtcg
    17461 ttattgacgg ctgtcaatcc ataagcttta catttgttta ttttcgaatt atggaatgga
    17521 attaatccga gcggtgtccc aaaacgatcg aattgcacca aactgccgtc ttcctggagc
    17581 acagttaaaa tacctttgcg atttatccgc agacttaccg gatggaccag cgcatagaca
    17641 ccatattcga tcccaccgaa ccaggtttgt tcattttttt cgacataaag gcgcaaaatc
    17701 tcagccgttt caccggataa aatgaagagt tcattaatct gcgaggctgt caaagacata
    17761 ggataaatta agcggcgaga atcactacgt aaatcctgag tcgcaatgta attcaattgc
    17821 cgatcatacc gcaccaagcg gtgattattc tggtcggcaa caattacatc taatccggaa
    17881 aaacacaaat cgcgtgggca atcgaattgt cccaatcccc atccaaaacc gccgttgcgg
    17941 ttcagaatat tgccattttg gtcgagacga taaacttcac tgaatttgct ttccagtaga
    18001 taaatatcgt cgaactcatt gacgctgatg gcattaaatt gcactccctc tctgactgat
    18061 tgcggaacaa tttctctctc aaacctgaat tgcgctttga tcgtacaatt ccagattaaa
    18121 ataaaaatta aaaggctgat tttacttgat cttttcatat tcaatcggtt ttcatagcgt
    18181 tagttaaata tacaatgtcg cccgcaaggt tcaaggcttt tttataaaag gatttgatgc
    18241 gttgaaacca atttagcctc ttcttaggag ctgcagaaat tggtttggct agtcacgcca
    18301 atacttagac cgctacgcag taagcacttg tctaaaaatc atagattcct aaatagcccg
    18361 gagggcttca agttttgtag aaattatcta acaagaaaat ctttctacct cggagaggtt
    18421 taacaaattt ttaaatcaat cttaatcaaa aaaatcaaac aaatattggg aatcaaatcc
    18481 aattccatat tctttcagaa attctatata ttcttcgcga aaagtccttt tagaatgatg
    18541 cgcttcttga ttttgaatat atctaaagac gtcatttatt tgtgatctac cataggaaaa
    18601 agcaccatat ccttcctgcc aattaaaatg cccataacat aacttatttt tattaatgaa
    18661 aattgaagta tttcgcttta tatcatgcat tgtatcagaa atggaaatat taggattgag
    18721 gccaatgaac aaatgagcat ggtcataatg tccattgact ataattgatt tatgccccaa
    18781 tttggtgtta ataccactca tgtattcaaa aactcgtttc agcatatctg aattaaggac
    18841 ggcatctctg ttttttacag caaaaactaa ctgtaaataa atttgggtgt atacccctga
    18901 tttcataaat aacctccttc ttaattttgc ttcattcttg atattgatga cattgatttt
    18961 aacagtatct ggtatatatc aaaatagcca atattcttta tcctctccga ggaataactt
    19021 ttctggcttt tttattctac aaaacttcga ccgctacgcg gtataagaaa aaaggtaaaa
    19081 tatcttgtcc cgccgagcgg gactccttct gagttaatct tatctaagaa catgcatctg
    19141 ctcgattcgc tgaaaccaat ttagcctctc cttaggagtt gcagaaattg gtttggataa
    19201 tcacttactt aaaaacgctc cgaaaacggc ttggattggt gacgttccac tccggatttt
    19261 tattcaaatt ctttttgata aaggtattga cgcgctcagc ccgatcaaag accgcgtcat
    19321 agctccagtt ggattctgga ttccagaaat ccggtgatgt gttggcttcg atgcgcgtca
    19381 gcacccgcca cagcgcagac ggatcataac cggaacgata ggcgtagatc attccgagtt
    19441 gatcggcttc gatctcatat tcaatttggc ggcgttttgt tgccgtctca tagcaattca
    19501 gggcaattag atcgagttca tcggcggtag cgtcttcggt gacaatctct tggtctaatt
    19561 cattaaatcc ctcgtcagct acaatcatcg ttttgcgttt ggcaatttcc tcagcgccat
    19621 gtttttgaac aacgtgacta atttcatgag ccagtatgac cgccagctcg gcttcatcct
    19681 gtaacagctc gagcaaaccg gaagtgacaa aaatcatacc gttgggaatg gcataggctg
    19741 acggatggct gtcttttaga atataaaatt taaagggata atcgtacata tcggtatttt
    19801 ccagaacgat attgcctacc agattgatat atttcaattg cgattgattg gcaaccaacc
    19861 cgcttgccgc aattctggaa gcggctgcca atccaatttt ttcatcttgc aatgagattg
    19921 agtgatcggt gttttctgat ttgattctct tatagcgttt gcggattttc tccgggtcgc
    19981 gaccgcgaaa agtctcttgt ctaaaacgct ggaattcagc cggagtaaac aattcaacat
    20041 catattgttc gataaaagcc ggatcgccgt cgtgaaaatt gacgaacttc tgggcgaaac
    20101 ctttgaccgc tccactggcg gaagctctcg aaatcatcat tgaaccattt ccactaatcg
    20161 aaccggtcaa aatgctaccg gttggttcat cactcgcaat aaccgcattt tcggaaatcc
    20221 agccgatttg tcccgcacat tgaatattca tccatcccga ttgagttcct acaacagcaa
    20281 cttggacgcc tttctgcaac atcccaatca gtggataata ggaaccgggg ccttgtcgga
    20341 aatgagtatt ctcacgtttg attaccacgg attcttcacc aaaaacggta acaattaaca
    20401 ccgacactac taaaattata agaataaaac gtttcatatt gcccctctcg ctttaatttg
    20461 tactttcgaa attctatttc attttgctca ataatttaac ttgtttcact tgacggtaat
    20521 attaaataca ccgtcttcgt caatcaaccg tggttcgtta attaattgaa cacagcgttt
    20581 ggcaaaaact ttagcgaccg aatcttctgg aaattgccga tttaattcta aaaatgccaa
    20641 ttcggcagtc ttgaatttgc gctcccgata ttgttgtaag gcttcattat gcaatttgag
    20701 catcttttcc agttcagaac ttaaatcgtt tataattccg ataatattat aaattgccaa
    20761 cggctcttca cgacctttca cccgaattaa atcaagttcg cggcagataa aattttcttt
    20821 taccaaggaa taggtcgtct cggaaataat attttgcgtg ccatagattt tgttgacgcc
    20881 ttccagacgc gccgcgagat taaccgtatc gccgatagca gtataatcca tacggttcgc
    20941 cgaaccgata ttgccgacga caaaattacc gctattaatc cccaatcgcg tgattaatgg
    21001 gatagtccgg ttcttgactt caatggtaaa agttgacaga tttttaaaat ccagcgccgc
    21061 attacaagcc atccgggcgt gttcgttagt cggaatcggt gccccgaaaa ttgccataat
    21121 tccgtcacca gtgtatttat cgagcattcc gtcgttcttg aaaattattt gttcgacttt
    21181 ggtaaaatat tggttcaaaa agccgacaat ttcaggtgga gtaaacagtt ccgcaattcc
    21241 agtaaaattc tgcaaatcag agaacattac cgtagcgacg atttccttgc cgcccatctc
    21301 aagacgctcg ggattcctgg ttaaaacctc aactacggtg ggatgcaaat agcgattgaa
    21361 aactttctta atcattttct tttcgcgccc ttcggtcaaa taattcacta atagaactac
    21421 cgtaatactc agcacaaaag caatttcggt ggttacaacc ggcataaaca ttttcaagcg
    21481 ctggaaaatc attacactca ttatcagcgg aataaataaa attatcaacg aaatcccagc
    21541 tgactgccag attttcaact tttgccaaag aaacgccaac agccagatta gaattagaga
    21601 aaatcccagc cagattataa atggtaaatt ggaaatataa tcgccccgga taatattcga
    21661 gaaaatagta gcataaatct cgactcccgg atatggctcg accgacgaaa atggtgtggt
    21721 cttcagatcg agtagtcccg ccgcagtagc gccgataaaa accgctttgt cctggaaaat
    21781 cgccggatca aggtctggct tttgaccatt ttgcacttgc aaataggaat tcaacaactg
    21841 ggcaaaagat acatattgaa aagtatttcc tgctccgcct ggtccatacc aataaatatc
    21901 gaaaaaacca ctattattaa gaggaacacg ccgttttccg gcgaagatag tatgcctcat
    21961 cgaatcataa tcaatagagg aaatgtcatc gtaaatcatc acagccgcga gcgccatata
    22021 gggcacgatt tgatgatcct tagcaaagaa aagcggaacc cggcgacaga ctccatcatt
    22081 atcggtgaaa aaattgaccg cgccgggata cgccatcgct ttttgaaatc gctcaatcgg
    22141 agccgtaaca ttcggatatt tgtaaacaat tcgctggggg acattgtaaa cgatttggcg
    22201 attaaattgg gtcggcagtt tttcaacact aagatgcgtc gaatcttcca tttgcattgc
    22261 cagaatgacc ttgccgctgg acagcattgc ccgcgcaaaa gctgaatcag catattcagc
    22321 ttgaacattc agccggtcaa tatcaggcgt ggaaaaaata atatcataga caatgacttt
    22381 tgcgccgcag gaattcaaat agttagtcgc catagcgtaa atatcgcggg gccaaggcca
    22441 aagaatctgg aaatttttct gaaaataatc gagactattt tggtcaatag caataattac
    22501 cgcctccgac ggatgttgtt caacctgaaa atcgcgatac aaaatatcat ttacttgatt
    22561 ttcccagcgt tgaatcaaac cgttatgata aagcaaaccg cttaataacg ttattattag
    22621 tgcaattccc agcccaatta gtattttttt agtattaatt ttcaccgctg gttttcatta
    22681 gaaaatttac ggttcagaaa tgatctccca atcatgttcc caattccatt gattgaagta
    22741 taaattgccg ccttcccatt cgacttctcc ccgctgagag tcaatggttt cactgcgcgg
    22801 ataaatcttc agtggataaa aatcagccgc cattccgtca ttaaaaatca ggcggtagct
    22861 gtccatcccg ctcaaataaa accatcggaa agattcatcg gccgtggcgc gccgaccata
    22921 agttacatcc atcggcaccc aaccgtaagg cgcaaggtaa atttcgcccc aatcgtgcat
    22981 actatcctga ggaggcttga attcccaacc cgactgccag cgcgccggaa ttccattcag
    23041 gcggcaaagg gtgataaaag tcaacaccat cataccgcaa tcgccatggc gattatttat
    23101 aacgtattga ggaatattat aaatcgtcga gtattcgcgc gccgaagccc aaactatatt
    23161 gacgtcaatc cactcaaata atttctgagc aatacgataa ggattggtct ccgaaccgac
    23221 gattctctgg gaaagttgcc ggatttcatc cgtaaatacg atatgcggcg gctcctcact
    23281 caggaatgga ttcaggtcgg gattcggttc taccggaatt actttctcgg cttttactgg
    23341 agtatatgaa ccataaattt cgtaagtgta ctcgaccgaa aaatgggtag tcgaatcctt
    23401 gactgccgct ttttcgaaat aaatcgtccg ttgaagggtt tgatcggtat tcgccagaat
    23461 ataaatcggc gggtcagttt ttagtaggcg gatattgacc tgccgatcag gaatttcgcg
    23521 cgggaaagga atccagcaac ggatgatttt tccttccggg acaacattcg gatgaacagt
    23581 taggctttgt tttattttca ttctaaccgg taaaacggta gatttttgcg tcgtttggac
    23641 ggcttggact attttgcgac aatgtgaatc tagatcgaac gccggttctc ttctagaatc
    23701 ggtaggtatc tgagaatcaa ctttttgctt aacggctttt aaagtgcgat ccaatcggaa
    23761 aagattcggc gcggcatcct tgaaatacat tttttcgccg tcaatcacga ggtattccaa
    23821 cgacttttcc gattcccaac gaaccaaatc agtgggcgtc acatcaggaa cgtactgctt
    23881 gatgtattcg ataacttcag cctgactctt attaaaatcc agacgaatgc gtttcattct
    23941 ttccagttca aattccagcg ttcgtctttc actaacgctg agagacggat tactaaataa
    24001 ttcccggcga attatttcct cggctttttt aaactcgccg ttttcgatta aagcccgaca
    24061 gtcgcttcct tcgcaatcta tccaaattat taagcaaagc aagccgatta ttcggtggat
    24121 atttttgatc attctttcaa ttctctacaa tttttaaaac cagctgaaaa ttagaacata
    24181 gaaacttctc tgtccagaaa tatttttacc tatca
//
LOCUS       DAOMJU010000028        21900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2220_length_21900_cov_21.242161, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000028 DAOMJU010000000
VERSION     DAOMJU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 21900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21900
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2220_length_21900_cov_21.242161"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..570)
                     /locus_tag="PLO58_04265"
     CDS             complement(34..570)
                     /locus_tag="PLO58_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQO74038.1"
                     /translation="MDFSELILKRRSVRKYLPKPVERELIDKCLDAARLAPSACNAQP
                     WYFIVIDNEEVKDRLCSAAFSGIYSSNKFVKNAPVIIVVITEQQSYQVKVGGFFRHLK
                     YALVDIGIAGEHLALQAAELGLGTCWLGWFSENGVRKALDLPKSTKIDIMFSLGYPAD
                     SDRPKKRRPLDEIRRYID"
     gene            complement(573..2273)
                     /gene="recN"
                     /locus_tag="PLO58_04270"
     CDS             complement(573..2273)
                     /gene="recN"
                     /locus_tag="PLO58_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQO74039.1"
                     /translation="MSLTKLKIDNFAIIDHIEVAFGPGLTILTGETGAGKSIIIDALN
                     LALGEKASPNVIRAGADVATVECTFDLTDLNPATKNILKSHNFYQENNPLTLKREVNI
                     NGRSRAWLNGETCLVSVLKEVGNLLVDMHGQHDHQSLLNPDTHIEFLDAFGDYAEWRE
                     QVARQFDELKLLFERQNLLQEQLRLNREKRELWEFQLQEIQKVAPQENEYETLLQEKT
                     ILENTAKIQQLSSDLAYQLLESGDSLYNQLQSVVKQLANLQKITGSFAEQLSRLEESQ
                     YLFQEIARQIAHFGDDLQFNPARLEIINQRLYSLQQLMKKYGPSLSDVVAYRQKLETD
                     LTESDDLEVQIDKNRELIKEHLEKFTRFAFELSEKRKQSAELFKIEIEKILERLGISG
                     ADFAVRLELMPDQNGWIIIDGQKYKAESTGIDKVTFEISTNRGEPRRPLAEIVSGGEV
                     SRIMLAIKTILAGRDHIPVLIFDEIDTGISGKIARKVGEELHELAKVHQVICITHLPQ
                     IAGLADEHFSVEKVTTNGRSQTQIRKLLPEEKVAEIAKLIGGRSVSEITLKQARELME
                     "
     gene            complement(2270..2650)
                     /locus_tag="PLO58_04275"
     CDS             complement(2270..2650)
                     /locus_tag="PLO58_04275"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQO74040.1"
                     /translation="MNRFWLKVPAILYAVLIFSLSSLTKTPPIEIPIWNVDKLVHMLE
                     YTIFGILLVLAFRSDQIGKPMRRANFQSIILGVLYGLTDEIHQYFVPGRTSSLADLGA
                     DALGILLGVWIFNRLSVFQKFREA"
     gene            complement(2650..4206)
                     /locus_tag="PLO58_04280"
     CDS             complement(2650..4206)
                     /locus_tag="PLO58_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQO74041.1"
                     /translation="MPEFILSNAEAAAVDRYAIEELQIPGTELMFKAGNFVALKAKKL
                     IQDIPNSRIDLFCGIGNNGGDGFVAALLLSEWGANCIIWLIGDPQNIKGDARHFYQKW
                     VESKQTIRYIKTAEDFEEYSDLPESDLIIDAMLGTGFRGEVQGIIADVIRRINNINLP
                     VIAVDIPSGVNGDTGEVRGDAVKADYTVTMGFLKRGLLFYPGKRFAGEIVVVDLGYPR
                     KAFDILKEKTFLIGKQDVKKVIPPIAEDTYKHRQGKVLIIAGSPGMTGAATLCALGAM
                     RSGAGLVVNAIPKSLNPIMEVKLTEALTAPLPESSQQTFCLESLTALQSRLEWSDVVV
                     FGPGVSDNVEVQEFGQQLITKCHKPLVIDADGLRIFHNNLELIGTIEDLTITPHRGEF
                     AMMTGIAVAEVNKNVIEVARDFVRKYPCTLVLKGAPSVVVSSTGVVAVNSTGNPALAT
                     GGTGDVLTGIIAAFRAQGLNSFDAAMAGTSIHGLAGDWGRKKFGVRAFIASDLLDYLP
                     LILRRLDRVI"
     gene            complement(4358..5647)
                     /locus_tag="PLO58_04285"
     CDS             complement(4358..5647)
                     /locus_tag="PLO58_04285"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO74042.1"
                     /translation="MKYVKQISVIILITLVVLSSLIGQDPLSEAYTLFQNGEYQKAFE
                     KCKPIVEKDPNYAGAVFLLGRIYFALGELDSSKVYLDKAIDLDRANTEFREIRTQIAA
                     FTTQLTEASRLVNNGDYEGAKNLYLEIIKANPNFADAYFNLAQVYIRLNDLTNASLYL
                     RKVIEMKPNEERYTKLLKNLVQQYLQEGENLRQRRNNEGALEKYKQALELDPNEFLAY
                     YFSGLAYYDMKNYDEAIKAINKGLEINPEHAKSYLLLGQIYEKTNKKAEALKAYEQAV
                     KIDPLFEAGWDRIGVLNYNMKNYEQSISAYKKLIEINPENPRYYEKLGQIYLDTNNPK
                     EAIINLEKVTQKDPKNVKAWLRLAQAYNNNGECAQAKTAAETALKIKAGDALALIELG
                     IAERCLGNTVAARQAFQLAAKDPQYRRYAEEHLKTVQ"
     gene            complement(5680..5765)
                     /locus_tag="PLO58_04290"
     tRNA            complement(5680..5765)
                     /locus_tag="PLO58_04290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5729..5731),aa:Leu,seq:gag)
     gene            complement(5813..6160)
                     /gene="secG"
                     /locus_tag="PLO58_04295"
     CDS             complement(5813..6160)
                     /gene="secG"
                     /locus_tag="PLO58_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQO74043.1"
                     /translation="MYTLLIIIHVIVSALLMLVILMQSSKGGGLSGTFGGAGGSSLFG
                     GRGAATFLSKLTTGLAIAFMVISLLIGLISAPRGEPTSIIRQEANRRTSPSAELPIPQ
                     GSSEQGTPVLPGN"
     gene            complement(6160..6939)
                     /gene="tpiA"
                     /locus_tag="PLO58_04300"
     CDS             complement(6160..6939)
                     /gene="tpiA"
                     /locus_tag="PLO58_04300"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQO74044.1"
                     /translation="MKQYIVAGNWKMHKDIQESRIFVSNILNKLLKHERATVILCPPF
                     TALFDVYNLIKNTSIALGGQNMHSEPKGAFTGEISGGMLISAGCQYVILGHSERRHIF
                     GETDDFIRDKVKQALQVGLKPIICVGEKLDERQSGETLEVVRRQCDSALSVVNEQEIK
                     RCVIAYEPVWAIGTGVVATPEQASESHSMIRQLLAAKYGQESAAGTSILYGGSVKSSN
                     AASLIQAQDIDGFLVGGASLIEDEFARIAEIVDEYVKHKEL"
     gene            complement(6946..8142)
                     /locus_tag="PLO58_04305"
     CDS             complement(6946..8142)
                     /locus_tag="PLO58_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQO74045.1"
                     /translation="MSRLTVDDLKVKGKRVIMRVDFNVPLDENLNVRDDFRIRAALPT
                     IQKLLNDGARLILMSHLGRPKGKPVPELSLKPVAECLQKYLKNKVYFAPDCVGIEVKN
                     LANSLQDGEVLLLENLRFHPEEEKNDPEFSAKLAELGEIYVNDAFGTSHRAHASTAGI
                     TNYIPVRAAGYLLEKELHYLQDMLENPERPFTAIFGGAKVSDKIDIIERLFGKVDNIL
                     IGGGMAFTFLKAKNYEVGKSLLEADKIDFALATLKKAEQMGCKIVLPTDVVVASEISD
                     TAVPEIVSVDQIPPDKIGVDIGPETTMIFCDIIEKSKTVLWNGPMGVFEVKQFSTGTE
                     SIARKLAEVTTKGVITVVGGGDSASAMKKFGLMDKVSHVSTGGGASLELLSGKELIPI
                     NLISKK"
     gene            complement(8231..8830)
                     /locus_tag="PLO58_04310"
     CDS             complement(8231..8830)
                     /locus_tag="PLO58_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQO74046.1"
                     /translation="MDSNVGLICADCWQKLQLFPAEAIKNKIIPESLDQVWPVFLFDE
                     LFQKIIHALKYQGNVSIGYEIGRRMALHLPSEIKDLQSACYVPIPLHPIKLRERGYNQ
                     SEAITRGLASNSGIRVETKLLKRIKNTATQTKLNAEERQANMEMAFAINRKNTVPSDN
                     AIILVDDVFTTGATLNSAAGVLRQSGFHKVIAVTAAAPL"
     gene            complement(8930..9793)
                     /locus_tag="PLO58_04315"
     CDS             complement(8930..9793)
                     /locus_tag="PLO58_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQO74047.1"
                     /translation="MRIGIDLGGTKVSIGLVDQNRLIGQPVRFMIAECKDADDLVKHM
                     KDAIDRSLKANGIVWQNLEMIGIGSPGPLDYKTGIILHTPNLVMLRNYPLGPKLEALT
                     GLPVLVNNDANCFALGEQRAGGGRGLEYVLGVTLGTGFGLGFIYRSEIFNGATGTALE
                     FAMTPYLDGVYEDYISGRGLARIYKSVVGKEIKPAEVTALAKKGDADALETWRIFGHH
                     LAQAMIVLVMVLDPDIIVIGGSVSQGWDFFYQTLETELRAGIHAGPAGHLKITRSELG
                     ELAAIIGAASL"
     gene            complement(9810..11180)
                     /locus_tag="PLO58_04320"
     CDS             complement(9810..11180)
                     /locus_tag="PLO58_04320"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HQO74048.1"
                     /translation="MRIKIILLLMCLLIVNSFAVEFDVDYAVFKGNEEVSVVEVYLLI
                     PRTLFQFVPNENEYCSYALIRVALVEGDSVRDIQEWQITDRVADTLQLAENQKIPEIA
                     TLQVKPGNYQLIIVVADLINRHQYRYDKQIEISDYSDDQLRLSDIQISSQISKTSQEN
                     KFSKYFGYDVIPNASAIFSQRQNLIYSFVEVYNLKVDPGKPSNYLVKYDIADLNGKVV
                     VSSDWRSRKKPGTSAVEINNLSIAGLPGGLYDLIIEVNDEATQKTVKKTKRFYILESK
                     SQLASLEMNAEAIQLEGKSEKELDDIFGPLRYIATETEIRRYKKSDVEGKKQIILHFW
                     DNRDPNPQTPINENKAEFEQRLQYVNQQFSTNQVPGWKSDFGRVFMIYGMPSEIERFP
                     SALESKPYQIWYYNEIEGGVKFYFVDKNGFGTYELVHSTARNELQDINWQRWISPYST
                     SPDLTY"
     gene            complement(11184..14705)
                     /gene="smc"
                     /locus_tag="PLO58_04325"
     CDS             complement(11184..14705)
                     /gene="smc"
                     /locus_tag="PLO58_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQO74049.1"
                     /translation="MYISKLTLYGFKSFLKKSEIQFGRGITSIVGPNGCGKTNIVDAI
                     RWVIGEQKASILRAERVTDVIFNGTATRRPLNLAEVSLTINNVSGRVPIAYSDVEITR
                     RLYRNGESEYFINRNLCRLKDITDLFIDTGMGANAYSIIELKMIENILSETPEERKRL
                     FEEAAGVNKYRVQRKAALRKLESTRDDLLRLNDIINEVDQNVKNLKRQIARYDKYQET
                     TQKLIEAEVILASRRIFDLRAKQAPILTDINAKQNLLEKISTELNAKESQEREQQKIL
                     NTIEIELQNKRNELETLRANKAKLEATGLLLKEQLRNNTQNSARLTATINALQKSIES
                     AQNRQKIIEQDLATNAQTLEEKRQAYKAAVEAQQEIENNYKKLSADLQALQDERFNAF
                     RQQAEQTAHLNNLKENINQREKELQKVIEQIATQTDSQARLAIALKELREKIDVLTVQ
                     RDELEKSLQNELSHQQSLTEQENRIRDELRHCESAFDKLNNQIQFYRSIIHSKEGFAP
                     ALQFVLDHPDEFPGIRGALSDLLSVDHQYYLAVEAVIKDIARLLVAESRSAALQTLEK
                     LNRLGKGRISIIPLDARWRTPALKSARNGLQPLLKFIKSDAQLENLKEMLFGNVYCCS
                     DDQFEELINNPELESYSIVTDRGRFRDANGWFSGGAEQSAANVLVGRQARLNELQEEI
                     KSVEKQRTALLEQLQAAQAEIRRHSEMREKLNRDRQQIETEIRQLTDVLRATESQQVQ
                     SAGALAALNDQKVMLTTAIANFKERLAKETPTQTNVDSQLAQMDQLISIKKTELDQVR
                     ANLDGHNQNLQNLRVELIGLENKARNLSDEKVSLERNLKNNLEQITKARQELAALELE
                     KSQLEKNLAENKLADAEAVTLVNQTESNYNQIGQEFQALRQTIEAINTEIMNLRHTKE
                     QNSEELKRLELASSQFIANINEISSVLWEKYNRTPLEEIPPDLPTETEARQVVERYRH
                     NLEQIGMVNMAVKEEYEQEYTRWKFLSDQRDDLVSSEKGLTEVITQIDQVAREQYLET
                     FNQIRSNFKTTFDIFFGGGEADLRLIGSDDPLEAEIDIWACPGGKKMRSLRMLSAGEK
                     ALTAIALLFAIYQVKPSPFCILDEVDAPLDDENTRRFTSVIRTFSENTQFIIVTHNKA
                     TMAIADALYGVTMAEKGVSQIVSVKLE"
     gene            complement(14718..15065)
                     /locus_tag="PLO58_04330"
     CDS             complement(14718..15065)
                     /locus_tag="PLO58_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HQO74050.1"
                     /translation="METDCIFCKIAAGKLGTEFLYESDEIVAFRDIRPKAPTHILIIP
                     RRHIPKITDLQESDEKLVGRMVTVANILAEKEGLKESGFRLIFNCGRDSGQEVFHIHL
                     HLLGGRPFHWPPG"
     gene            complement(15055..15792)
                     /locus_tag="PLO58_04335"
     CDS             complement(15055..15792)
                     /locus_tag="PLO58_04335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HQO74051.1"
                     /translation="MHAYQFYISMEKIQEGTFDLEGDEYRHCCRVLRKIRGDSIDVFA
                     GNGRRWSARIEKISNNKAECRIMEEYPPQSRLKPEIILGVGLLKNNLMDDVVVAATAL
                     GVAEIMPLYTTHSIKNKINHERLAKLALSALKQSGLAFLPIISDCLTLDKWFNLTEEI
                     PLKLIAEQHASANLNRIQQLQTVEQVALMIGPEGGFEENEIEEAQRFGFNKVNIFPYR
                     LRTELAVVTALAGIRTLTLKNEAYNGN"
     gene            complement(15792..16481)
                     /locus_tag="PLO58_04340"
     CDS             complement(15792..16481)
                     /locus_tag="PLO58_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74052.1"
                     /translation="MNTISKYKVGLILLGLVMTIPLSAAKKINFTEAKARYSGLTIFQ
                     TNEGSGIGGYFEYSLKNANRLAAQVMFTVATGDDYPLYYYDYYYDQYYYYESASKRRL
                     SFLHLLGGYKRILFADKIANDFRPYLECLGGAVMALDPPNIPDFSDRIKKMTTAWAPG
                     FQIGGGIDFLSGPKTLVSIFAGYDYLKFAQKIDPPQVYYDEDGNPLENRYSGQKDFSG
                     LVIKISFGKKL"
     gene            complement(16649..17686)
                     /gene="asnA"
                     /locus_tag="PLO58_04345"
     CDS             complement(16649..17686)
                     /gene="asnA"
                     /locus_tag="PLO58_04345"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HQO74053.1"
                     /translation="MNSGLFIPKNYRTILTTVETEKAILALKDFFQQNLAFELDLIRV
                     TAPLFVKAGTGINDDLNGIEQPVSFTVKEMANTQVEIVQSLAKWKRMMLTDLGIQPGK
                     GLYTDMNAIRPDDILDNLHSIYVDQWDWEKAITPEQRNLDFLKKTVSTIYDVILRTEK
                     FVCHEWNISPELPEKITFIHSEDLLDQYPDLPPRAREKEACKKFGVIFLIGIGGQLQN
                     GEPHDGRAPDYDDWSTPTVNGYHGLNGDIIVYNRVLDCAFELSSMGIRVDREALLRQL
                     KIRQVEERQNLYFHQRLLKGELPLSIGGGIGQSRLCMFYLRKAHIGEIQCSIWPEEMV
                     KLCQRNNIFLL"
     gene            17894..18973
                     /locus_tag="PLO58_04350"
     CDS             17894..18973
                     /locus_tag="PLO58_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQO74054.1"
                     /translation="MPVNQIQLMRFKSDEPVDQTKNRLKKLFKSAGLDRVIQAGDITA
                     IKIHFGEKGNVTHVPAAYVEPIVRMIKSCGARTFLTDTTVLYKSVRQNAIDHLQLAYD
                     NGFTYEKVGAPVIIADGIIGRNETEIEVSAPLNKTVSLATELVAANSIIAITHVTGHL
                     STGLGATIKNLGMGMSSRRGKLAQHSVSKPIIKSNKCTACGICMKWCPANAIEIPDKF
                     AIIDQDICIGCGECLTVCRFDAVHFKWNNSSTRLQQQIAEHALGIVRQKSGRIGYLTF
                     LTNMTKDCDCLPGKQVPIAPDIGVLAGFDPVAIDQAVIDLTNQVNNQNIAQLAHPEID
                     YSIQLDYGEKIGLGCRKYQLIETEF"
     gene            19034..20011
                     /locus_tag="PLO58_04355"
     CDS             19034..20011
                     /locus_tag="PLO58_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQO74055.1"
                     /translation="MIDVFFYEAFQEEAELIERFLPENITAGFDWRTIQESEHPAPPA
                     KIISIRTQSIVSVSWLPELSAILSRSTGYDHLILLAQKAGSRLQVGYLPLYCNRSVAE
                     QAILLALALLRKLPQQLQQFQKFHRDGLTGRECAGKTLLVVGVGNIGHEIVKIGIGLG
                     MQVLGVDIIKKHSDVNYISIEQGLPKADIIICAMNLTSENHNYFNADRLRKAKPGVVF
                     VNIARGEQSPSTELLKLMQEGHLGGIGLDVYNVESELAVALRSGSTSPNKEVQATLAL
                     AKLPNVILTPHNAFNTYESVERKAAQSITQIQHLLENGRFLWPIPADGK"
     gene            complement(20263..21870)
                     /locus_tag="PLO58_04360"
     CDS             complement(20263..21870)
                     /locus_tag="PLO58_04360"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HQO74056.1"
                     /translation="MKQSVVCKILVIVVWISFIPLVGIAREKADWEGGYPDGCTSVMV
                     GKKASRDGSVMTSHTCDSHRTRSSFDIVPTKKYSKGEMVTMLKRIDCDSLAMPAYQYL
                     AIGEIPQVVSTYGFINTAYPCMNEKQLAIGESTFGGRECLRSDNGLIDCQQLVRLMVE
                     RCSTAREAIRMAGELTKKYGWIDEGECLTIADKNEVWALEIVGPGKGNCGSIWVAQRV
                     PDDHVSVNANGSRIRQIDLKKPDYFMASENVFKVAQDSGWWNPKEGPFEFAYAYAPES
                     RQTFSARRREWRVLDLVAPSLKLHPDAENFPFSVKPDTLVDLAKMIRIFQDYFEGTDY
                     NFVKNITWTNSEGKTEISPFANPFMPYDMNHIFKINGGWGWRGERPIARWYTMYATIT
                     QSREWLPDEIGGVVWLALDNVATSIYIPLYCSIYDVAPSYKVDGRTKGFNRESAWWAF
                     NRLGTLAAQRWGDMRYDVAAVWGPMQSELLRRQNEIESQALRQYRHSPEAARHFLTKY
                     SIDWGAKVVNRAWKLGDELWTKYDEQF"
BASE COUNT         6061 a         5099 c         4351 g         6389 t
ORIGIN      
        1 acagcctttc caggctgttc agtgttttaa attttaatca atatatcgcc ggatttcatc
       61 cagcggtcgg cgctttttcg ggcggtcgga atccgccgga taaccgagac taaacattat
      121 atcaatttta gtagatttgg gaagatcaag agctttacgt acaccatttt cactgaacca
      181 acccagccag caagttccga gccccagttc agccgcttgc aaggcgagat gttcaccagc
      241 aataccaata tccaccaaag catatttgag atggcgaaaa aatccaccaa ctttaacctg
      301 ataagattgt tgttcggtaa taactacgat tatcaccggt gcatttttca caaatttatt
      361 cgaagaataa atcccgctaa aagcggcgct acacaaccgg tctttcactt cctcattatc
      421 aattacaata aagtaccagg gttgagcgtt gcaagccgaa ggtgctaaac gcgcggcatc
      481 caaacatttg tcaatcagtt cacgctctac cggtttcggc aaatatttac gaacactgcg
      541 cctctttaat attaattctg aaaaatccat cttcactcca ttaattcgcg cgcctgtttt
      601 aaagtaattt cgctgactga acgaccgcca atcaatttag cgatttcggc tactttttcc
      661 tcgggaagta atttacgaat ttgagtctga ctgcggccgt tcgtagtaac tttctccacg
      721 ctaaaatgtt cgtcagccag accggcaatc tgaggcaaat gggttataca aatgacctga
      781 tgaaccttgg caagttcatg taactcctca ccgacttttc gcgcaatttt ccccgagata
      841 ccggtatcta tttcatcgaa aatcaacacc ggaatatgat cgcgtccagc caaaatcgtc
      901 ttgatagcca gcataatgcg agaaacttcg ccgcctgaaa caatttctgc cagcggtcgg
      961 cgcggttcac cgcgattagt acttatttca aaggttactt tatcaatgcc cgtcgattcc
     1021 gctttgtatt tctgaccatc aattataatc cagccatttt ggtccggcat caattccagg
     1081 cgtacggcga agtcggctcc gctgattccg agacgttcca gaattttttc tatttcgatt
     1141 ttaaataatt cggcactttg tttacgtttt tcggataatt caaaggcaaa tcgagtgaat
     1201 ttttccagat gttctttaat aagttccctg tttttgtcaa tttgaacctc gagatcatcg
     1261 ctttcggtca gatcggtctc tagcttttgc cgataggcga cgacatcgct cagtgatggt
     1321 ccatactttt tcatcaattg ttgaagacta tacaaacgct gattgatgat ctctaaccgc
     1381 gcgggattaa attgcagatc atcgccaaaa tgggcgattt gccgggcgat ttcctggaat
     1441 agatattgac tctcctccaa tcgggaaagt tgctctgcaa atgagccggt aattttttgc
     1501 aaattggcaa gttgcttcac aacgctctgt aattggttat aaagtgagtc gcctgactcc
     1561 agtaattgat atgccaaatc cgaactaagt tgctggattt tagcggtatt ttcgagtata
     1621 gttttttcct gaaggagggt ctcatattca ttttcctgag gagcaacctt ttggatttcc
     1681 tgcaattgaa attcccagag ttcgcgcttc tcgcgattga gtcgtaattg ttcctggagt
     1741 aaattttggc gctcaaaaag tagtttgagc tcatcaaatt gccttgcaac ctgttcgcgc
     1801 cattcggcgt aatccccaaa tgcatccaaa aattctatat gggtatccgg attcaaaagc
     1861 gactggtgat catgctgacc gtgcatatca accagcaaat tgccaacctc tttaagaaca
     1921 ctcaccaggc aggtttcgcc attaagccag gcacgactgc gaccgttaat atttacctcg
     1981 cgtttcaagg tcaaaggatt attttcctgg taaaaattat gagatttcag aatattttta
     2041 gtggctggat tcaaatcggt gagatcaaag gtgcattcta ctgttgcgac gtctgcaccg
     2101 gctcgtataa cattcggact ggctttttcc cctaacgcta gattcaaggc atcaataata
     2161 attgacttac cggcaccggt ttctcctgtt agaatcgtca atcccggccc gaaagcaact
     2221 tcaatatgat caatgatcgc aaaattatca atttttaatt tagtcagact catgcttctc
     2281 taaatttctg aaaaacactc aagcggttga aaatccatac gcccagtaaa attcccagcg
     2341 catcagctcc aagatcagcc aatgaactgg tgcgaccagg gacaaagtac tggtgaattt
     2401 catcggttaa gccatataaa acacccagaa taatgctctg gaagttagcg cgccgcatcg
     2461 gtttaccaat ctgatcgctt ctaaaagcca acactagtaa aatcccaaat attgtatatt
     2521 ccagcatatg aaccagctta tcaacgttcc agattggaat ctcaatcggc ggagtttttg
     2581 ttaaagatga taaactaaat ataaggacgg catataaaat tgccgggact ttcagccaga
     2641 agcgattcat taaatcaccc tatccagccg acgcaatatc agcggtaaat aatccagtaa
     2701 atcactagcg ataaatgccc tgactccgaa ttttttacgt ccccaatcac cggctaatcc
     2761 atgaatggaa gttcctgcca tagccgcatc gaaagaattt aagccctgag cccgaaaagc
     2821 cgcaattatg ccggttagaa catcccccgt gccgccagtc gccaaagccg gattgccggt
     2881 cgaattgacc gccaccactc ccgtagaaga aacaaccaca gacggtgcgc cctttaacac
     2941 caaagtacaa ggatactttc gtacgaaatc gcgagcaact tcaatcacat ttttattcac
     3001 ttctgcgact gcaatccccg tcatcattgc aaattcgcca cgatgaggag ttatagttaa
     3061 atcctcaatc gtaccaatta attctaaatt attatggaaa atacgcagac catcagcatc
     3121 aattaccaat ggtttatgac attttgttat caactgttgc ccgaattcct gaacttcgac
     3181 attatcggaa actcctggac caaagacaac cacatcactc cattccagtc tggattggag
     3241 tgccgtcaaa gattccagac aaaaagtctg ctgagacgat tccggaagtg gtgcggttag
     3301 cgcctcggtt agtttgactt ccattatcgg attcagactc ttgggaatgg cattcaccac
     3361 cagtccggcg ccactacgca tagcgcctaa tgcgcaaaga gtcgccgctc cggtcattcc
     3421 gggcgaacca gcaattatca aaacttttcc ctgacgatgt ttgtaagtat cttcggcaat
     3481 cggtggaata accttcttga catcctgttt accaattagg aaagtttttt cttttaaaat
     3541 gtcaaaagct ttacgcggat aaccgagatc tactacgaca atttcaccgg cgaatctttt
     3601 gcccggataa aaaagcaaac cacgttttaa aaaacccatc gtcacggtat aatcggcttt
     3661 gacggcatct cctctgactt ctcccgtatc tccatttacc ccagagggaa tatccactgc
     3721 aattaccggt aaattgatat tattaatacg acgtatcaca tcggctataa tcccctgaac
     3781 ttcaccacgg aagccagttc cgagcatcgc atcaattatc aaatcggatt ccggcaagtc
     3841 ggaatattct tcaaagtctt ccgctgtttt tatataacgg atagtctgct tgctctccac
     3901 ccatttctga taaaaatgac gtgcatcgcc tttgatattt tgcggatcac caatcagcca
     3961 aataatgcaa ttagcgcccc attcacttaa taacaaagcc gcgacaaacc cgtctccgcc
     4021 gttgtttcca attccgcaga aaagatcaat gcgactgttg ggtatgtcct gaatcaattt
     4081 cttggctttc agggcaacaa aattgccggc tttgaacatt aattcagtgc cgggaatttg
     4141 caactcctca attgcatagc gatcaaccgc cgcggcttca gcatttgaaa ggatgaattc
     4201 aggcatagta gtcggttctt ttactaatga ttgttttcat atttcgcttt taaaattact
     4261 caaaaacctc tttaagccat agaatttttc ataaaagagt tatccgaata aaaaagggtg
     4321 agactgactc acccttaatt attctgacta gaaaatttta ttgaaccgtt ttcagatgtt
     4381 cttctgcgta gcgccgatat tggggatctt tggcggctaa ttgaaaagcc tgacgagccg
     4441 cgaccgtatt tccgagacaa cgttccgcaa ttcccagttc gattagagct agtgcatcac
     4501 cggctttaat tttcaaagcg gtttcagccg ccgtcttagc ctgagcacat tccccattat
     4561 tattataagc ttgggcaagt ctcaaccaag ccttcacgtt ctttggatct ttctgagtaa
     4621 ccttttccaa attgataatc gcttccttgg gattattggt atccagataa atctgcccca
     4681 gtttttcata gtagcgtgga ttctcgggat tgatttcaat caatttttta taggcactaa
     4741 tggactgctc ataatttttc atattgtaat tcaatacccc gattcgatcc caaccggctt
     4801 caaaaagtgg atcaatttta actgcttgtt cgtaagcttt tagggcttca gcctttttgt
     4861 tggtcttctc gtagatttga ccaagcaata agtaggactt ggcatgttcg ggattgattt
     4921 ccaagccttt attgatagct ttgatggctt catcataatt cttcatgtca taataagcca
     4981 gcccactgaa ataataagcc aaaaactcgt tcggatcgag ttcgagtgct tgtttgtatt
     5041 tctccagcgc tccttcatta ttacgtctct ggcgcaggtt ttcaccttcc tgtaagtact
     5101 gctgaaccaa gttctttaat agttttgtat accgttcttc gttcggtttc atttcgataa
     5161 cttttctcaa gtacagtgag gcattcgtca aatcatttag acgaatatat acttgagcca
     5221 gattgaaata agcatccgca aaattgggat tagccttaat aatttcgagg tataaatttt
     5281 tagcgccttc gtaatcacca ttatttacta gtcgagaagc ttctgttagc tgagtagtaa
     5341 aggctgcaat ctgggttcta atctctcgaa actcggtatt ggcacgatct aaatcaatcg
     5401 ctttgtcaag gtaaaccttg gagctatcca gttctccaag tgcaaaataa atccgcccca
     5461 aaaggaaaac agccccagca taattgggat ctttctcaac aataggttta catttttcaa
     5521 atgctttctg atattctccg ttctgaaaga gagtataagc ttctgagaga ggatcttgtc
     5581 caatcaggct actcaacaca accaaagtaa ttaaaatgat tacactgatt tgttttacat
     5641 atttcatcat ttaccccatt ttattggttt tcattttcgt gccgaggacg ggacttgaac
     5701 ccgtacaagc aaatccgccc acatgcccct caaacatgcg tgtctaccag ttccaccacc
     5761 tcggcaatta tcaactattt caaaaagcta tctggataaa tttgcaatta atttaattac
     5821 caggcaggac gggagttccc tgttcgctac taccttgcgg aatcggcaat tcagctgaag
     5881 gtgaggtacg acgattagcc tcttgacgga taatactggt gggttcgccg cgcggagccg
     5941 aaattaatcc aattaaaaga gaaataacca taaaggcaat cgccaaaccg gtcgttaact
     6001 tcgaaagaaa agtagccgcg ccacgtcccc cgaataaaga actgcctccg gcgccaccaa
     6061 acgtacctga caatccgcct cccttgctgg attgcattaa aattaccagc attaataagg
     6121 ctgacacaat tacatgaatt attattaaga gggtatacac tataactcct tatgttttac
     6181 gtattcgtca acgatttcgg caatccgggc gaattcgtct tcaattagac tggcgccacc
     6241 gaccagaaat ccatcaatat cttgcgcttg aatcaaactc gcggcattcg aagatttaac
     6301 gctaccgccg tacagaatcg aagtcccagc cgcgctctcc tgcccatatt ttgcggctaa
     6361 aagttgccga atcatagaat gagattccga agcttgctcg ggtgtagcga caacaccggt
     6421 tccaatcgcc cagaccggtt cataagcaat cacacatcgc tttatttcct gttcattgac
     6481 tacgctcaag gctgaatcac attgtcggcg gacaacctcg agggtttcgc cggattgacg
     6541 ctcgtccaat ttctcgccaa cgcagataat cggtttcaaa ccaacctgca gggcttgttt
     6601 gactttatcc cgaataaagt catccgtttc gccgaaaata tgtcgccgct ccgaatgccc
     6661 taaaatgaca tattgacagc cggccgaaat cagcatccca ccggaaattt cgccagtaaa
     6721 tgcacccttg ggttcgctgt gcatattttg gccgcccagc gcgatggaag tgttttttat
     6781 taagttataa acatcaaaaa gcgccgtaaa aggaggacat aaaataaccg ttgctctttc
     6841 gtgtttcagc aacttattga gaatgttcga cacgaaaatg cgagactcct gaatgtcctt
     6901 atgcatcttc cagttaccgg ctacaatata ttgtttcata atcaactact tctttgatat
     6961 aagattaatc ggaattagtt cctttccgct caacaattcc agcgaagcac caccgcctgt
     7021 cgaaacgtgg gaaaccttgt ccattagtcc gaattttttc atcgcggaag ccgaatcgcc
     7081 accaccgacg acggtaataa cgcctttagt cgttacttcc gccaatttgc gtgcgattga
     7141 ttctgtaccg gtagaaaatt gtttgacttc gaagacgccc atcgggccat tccacaggac
     7201 tgttttgctt ttttcaatta tatcgcagaa aatcatcgta gtctcaggac cgatatcgac
     7261 accaatttta tctggtggaa tttgatccac ggagacgatt tccggcaccg ccgtatcact
     7321 gatttcagat gcaacgacta catcggtcgg caaaactatt ttacatccca tctgttcggc
     7381 tttcttcaaa gtcgccaatg caaaatcgat tttatcagcc tccagcaatg atttaccgac
     7441 ttcataattt ttggctttta gaaaggtaaa tgccatcccg ccaccaatca aaatattatc
     7501 aactttacca aacaaacgct cgataatatc aattttatca ctgacttttg ctccgccgaa
     7561 aatagccgtg aacggtctct ccggattctc aagcatatcc tgcaaataat gcaactcctt
     7621 ttcaagcaaa taaccagccg cccgcactgg gatatagttc gtgataccgg cggtggaggc
     7681 atgagctcga tgacttgtac cgaaagcatc gttgacgtaa atctcgccca actcagctaa
     7741 cttggcagaa aattcgggat catttttctc ctcttctgga tgaaaacgca gattctccag
     7801 cagaagaact tcaccatcct gaagcgaatt ggctaagttt ttcacttcga tcccgacaca
     7861 atcaggcgca aaatatactt tgttcttcaa atatttctga agacattcgg cgaccggctt
     7921 aagactcaat tccggcacgg gtttaccttt tggacgaccg agatgcgaca tcaatatcaa
     7981 acgggcgccg tcattcagta acttttgaat agtcggtaaa gccgcccgaa tgcgaaaatc
     8041 atctcgaaca ttcaaatttt catcaagcgg gacattgaaa tcaacccgca taatgactct
     8101 tttacccttt acttttaaat catcaacagt caatcttgac atatttcacc tttagtttat
     8161 tcattgttta aaataacttt caattttaat ttcttagagc aattattcca aggaaaaata
     8221 ttgattcctg ctaaagtgga gcggcggcag ttaccgctat aactttatga aaaccggact
     8281 ggcgtagtac tcccgcggct gaatttagag tagcaccggt tgtgaaaacg tcatcaacca
     8341 gaataatagc attatcggaa ggaacagtat ttttccgatt aattgcgaag gccatttcca
     8401 tattcgcctg gcgttcctca gcatttaatt tggtttgagt tgccgtattt ttgattcgct
     8461 taagtaattt ggtttcaact ctaatgcctg agttcgacgc caatccgcgt gtaatcgctt
     8521 cggattgatt atatccgcgt tcccgcaatt ttatcgggtg caaaggaatc ggaacgtaac
     8581 aagccgattg gaggtctttt atttcagatg gcaaatgaag agccattcgt cgtccaatct
     8641 cgtaaccaat cgaaacattt ccctgatatt tcaaagcgtg aattattttc tgaaacaatt
     8701 catcaaatag aaaaaccggc cagacctgat caagactttc cggaataatt ttatttttta
     8761 tcgcttccgc cggaaataat tgtaattttt gccagcaatc agcacaaatc agccctacat
     8821 ttgaatccat ccttttttca caacctttgc agatcggcgg aaatagcaaa gaagtaacac
     8881 cagtcacgag agttttgaaa aagtgcataa ttaaaacgat cagctttttt taaagactag
     8941 ccgcgccgat aatagccgcc agttcaccta attcactgcg ggtaattttc agatgaccgg
     9001 ctggaccggc atgaattcct gcccgcagtt cggtttccag cgtttgatag aaaaaatccc
     9061 aaccttggga cactgatccg ccaattacga taatatccgg atcgagcacc ataaccagaa
     9121 ctatcatcgc ttgggcaaga tgatggccga atattcgcca ggtttccaga gcatctgcat
     9181 cacctttttt agctaaagcg gtaacctcag ccggtttgat ttcctttcca accacagatt
     9241 tatagatgcg cgccaagcct cggccggaaa tataatcctc gtaaacacca tcaagataag
     9301 gagtcatcgc aaactctaaa gccgtcccgg tagctccatt gaaaatttcg ctacgataaa
     9361 tgaatcccaa cccaaatccg gtacccagag ttacaccgag aacatattcg agaccacggc
     9421 cacctccggc gcgttgttct ccgagcgcaa aacagttggc atcattattc accagcaccg
     9481 gtaaaccggt caaagcctcc agctttggtc cgagtggata attgcgcaac atcaccagat
     9541 taggagtgtg taaaattatc ccagttttat aatcgagcgg tccgggacta ccgattccga
     9601 tcatttccag attctgccag acgatcccgt tagctttaag acttcggtca atagcgtctt
     9661 tcatatgttt aactaaatcg tcggcgtctt tacattcggc aatcataaaa cgaaccggtt
     9721 gaccgataag ccgattttga tcaaccaatc caatcgaaac ttttgtgccg ccgaggtcta
     9781 ttccaattct catcctttta cctaccttcc taataagtta aatctgggct ggtggaataa
     9841 ggcgatatcc agcgttgcca gtttatatcc tgcaattcat tgcgagcagt cgaatgaacc
     9901 agttcgtaag taccaaatcc atttttatca acgaaataaa atttcacgcc gccttcgatt
     9961 tcattataat accagatctg atagggtttg ctctcaagcg cactgggaaa gcgttcaatt
    10021 tcggagggca ttccgtaaat cataaaaacc cgtccaaaat ccgatttcca accggggact
    10081 tgattggtag aaaattgctg attgacatat tggagacgct gttcgaattc ggctttattt
    10141 tcattaattg gtgtttgggg attaggatcg cgattatccc agaaatggag gataatttgc
    10201 tttttccctt caacatcgga ttttttgtag cgtctaattt ccgtttcggt ggctatgtat
    10261 cgtaaggggc caaaaatgtc atcaagttct ttttcagatt tgccctccag ctggatagcc
    10321 tcggcattca tttcgagact ggcaagctga gatttgcttt cgagaatgta aaaccgtttg
    10381 gtcttcttca ctgtcttttg agtcgcctca tcatttactt caataattaa atcatacaaa
    10441 ccacccggta agccagcaat actgagatta ttgatttcga ccgccgaagt gcccggcttc
    10501 tttcgagaac gccaatcact acttactact acttttccat ttaagtccgc aatgtcatac
    10561 tttactagat aattcgacgg tttaccggga tcaactttca agttataaac ttcgacaaaa
    10621 gaataaatca aattttgcct ttggctaaaa atggcactgg cattcggaat cacatcataa
    10681 ccgaaatatt tactgaattt gttttcttga ctggttttag aaatctggct actaatctga
    10741 atatcactga gccttaattg atcatccgaa taatctgaaa tctcgatttg cttatcataa
    10801 cgatactgat gtcggttaat cagatccgct acaactatga tcaactgata attgccgggt
    10861 ttgacctgca aagtagctat ttccggaatt ttctgatttt cagctagttg taatgtatca
    10921 gcaacgcgat cagtaatctg ccactcttga atatcgcgca cggaatctcc ctctaccaga
    10981 gcaactcgaa taagagcata ggaacaatat tcattttcat taggaacaaa ttgaaataac
    11041 gtccgtggaa tcaggagata aacctcaact acactgactt cctcattgcc tttgaagacc
    11101 gcataatcta catcgaattc tacggcaaat gagttcacaa tcaacaaaca cattaacaat
    11161 aaaattattt tgatacgcat aggttactcc aatttaaccg aaacgatttg tgagacacct
    11221 ttttcagcca tcgtgactcc gtataaagcg tcggcgatcg ccatcgtcgc cttattatga
    11281 gtaacgatta taaactgggt attttccgaa aaagttcgaa tgacgctggt aaagcgacgc
    11341 gtattttcat cgtcaagagg cgcatcaact tcatccagaa tacaaaaagg actgggtttg
    11401 acctgataaa ttgcaaatag taaagctata gccgtgaggg ctttctcacc ggcagaaagc
    11461 atccgcagtg agcgcatttt cttaccaccc gggcaagccc agatatcaat ctcggcttcg
    11521 aggggatcat ccgaaccaat tagacgcaga tcggcttcgc cgccgccgaa gaaaatgtcg
    11581 aaagtggttt taaaattgct ccggatttga ttaaaagttt ccaaatactg ttcacgggcg
    11641 acctggtcaa tctgcgtaat cacttctgtc aatccttttt ccgaagatac taggtcatca
    11701 cgctgatcac ttagaaattt ccagcgtgtg tattcctgtt catactcttc cttgactgcc
    11761 atatttacca tgccgatttg ctccaaattg tgtcggtaac gttcgacaac ctggcgggct
    11821 tccgtttcag tgggcaaatc gggcggtatt tcttctaatg gcgtccgatt gtatttttcc
    11881 cagaggacgg agctgatttc attgatatta gcaataaact gagaactagc cagttccaga
    11941 cgtttcagct cctcactgtt ttgttctttg gtatgacgca aattcataat ttcggtatta
    12001 atcgcttcaa tcgtctgccg tagtgcctga aactcttgtc caatctgatt gtaattagat
    12061 tctgtttgat tcaccagagt tactgcctcg gcatcggcga gtttattttc tgccaaattc
    12121 ttttccagtt gactcttttc caattctaaa gcggcgagtt cctgccgggc ttttgtgatc
    12181 tgctcaagat tatttttcaa gtttcgttct aaagatacct tttcgtcaga taaattgcgg
    12241 gctttatttt cgagaccaat tagctcaact cgcaggtttt gcaaattttg attatgccca
    12301 tcgaggttag cccgtacctg gtcgagttcg gtctttttaa tagatataag ttgatccatc
    12361 tgggctaatt gactgtcaac attagtttgg gtgggggttt ctttagccag cctttctttg
    12421 aaattggcaa tcgcggtggt aagcatcact ttttggtcat taagagccgc cagagcccca
    12481 gccgactgca cttgttgtga ttccgtagcc cgcaagacat cggtcaattg gcgaatttca
    12541 gtttctatct gctgacgatc gcgattcagt ttttcacgca tttcactgtg gcgacgaatt
    12601 tcagcctgag ctgcttggag ctgttcgagc agagcggttc tttgtttttc aaccgattta
    12661 atttcttctt gtaattcatt cagtcgggct tgacgaccga ccagcacatt ggcggctgat
    12721 tgttcggcgc cgccggagaa ccagccattc gcatcgcgaa aacgcccgcg gtcagtaaca
    12781 atcgaatatg actccagctc tgggttattg attagttctt caaattgatc atctgagcaa
    12841 caatagacat tgccaaaaag catctctttc agattctcca gctgagcatc cgatttaata
    12901 aattttagaa gtggctgtaa accatttctg gccgatttaa gggcaggcgt acgccatctg
    12961 gcatctaatg gaattattga tattcgtcct ttcccaagac gatttaattt ttcaagggtt
    13021 tgcaaagccg ctgaacggct ttcggcaact agtaatcggg cgatatcctt gattacggct
    13081 tcaacggcta aataatattg atgatcaacg gataataaat cggataaagc gccacgaatt
    13141 cccggaaatt catcaggatg atcgagcacg aattgtaagg ctggcgcaaa cccttcttta
    13201 gagtggataa ttgagcggta aaattggatc tgattgttca atttatcgaa cgccgattca
    13261 caatgacgca attcatcccg aatgcgattt tcctgttctg ttaaagactg ctgatgagat
    13321 aactcgtttt ggagagattt ttccagctcg tcgcgttgaa cggtcagcac atctattttt
    13381 tcacgaagtt ccttaagtgc gattgccaaa cgagcctggc tgtcagtttg agtagcaatt
    13441 tgttcgataa ctttttgtaa ttctttttcg cgctgattga tattttcttt aagattattt
    13501 agatgggcag tttgctcggc ttgttgccgg aaggcattaa agcgctcgtc ctgaagtgcc
    13561 tgcaaatccg cacttaattt tttataattg ttttcgattt cctgctgggc ttcgactgcc
    13621 gccttgtaag cctgacgctt ttcttcaagg gtttgggcgt tggtagccaa atcctgttcg
    13681 attattttct ggcggttttg cgctgattcg atacttttct gtaaagcatt aatcgtggcg
    13741 gttaggcgag cgctgttttg agtattgtta cgaagttgct ctttgagaag tagtccggtg
    13801 gcttccaatt tggctttatt agcccgcaaa gtctcgagtt cattacgctt gttctgtaat
    13861 tcgatttcga ttgtatttag aattttttgt tgttcgcgtt cttgtgattc tttggcattc
    13921 agttcggtgc taattttttc cagtaaattt tgttttgcgt taatatcggt taaaatcggc
    13981 gcctgttttg cgcgtaggtc gaagatgcgg cgactggcta agattacttc ggcttcgatt
    14041 aatttctgag tggtttcctg atatttatca tagcgcgcaa tttgcctttt caaatttttg
    14101 acattttgat caacttcatt gataatatca ttcagacgga gcaagtcgtc gcgagttgat
    14161 tcgagtttac gcaaggcggc tttacgctgg acgcggtatt tattgacacc ggcggcttcc
    14221 tcgaagagac gcttgcgctc ttccggcgtt tcactgagaa tattctcgat cattttgagt
    14281 tcgattattg aataggcatt cgcccccata ccggtatcaa taaatagatc ggtaatgtct
    14341 ttcagacgac aaagattgcg attgataaaa tattcacttt caccgttccg gtataatcgc
    14401 cgcgtaattt cgacatcgga atatgcgatc ggtactcgtc cactaacatt attaattgtc
    14461 aaacttactt cggctaaatt cagcggtcgg cgggtagcgg ttccattgaa aattacatcc
    14521 gtgacgcgtt cggcgcgcaa aatgctggct ttctgttcac caataaccca gcggattgca
    14581 tccacgatat tggttttacc gcaaccgttc ggtccaacga ttgaagtaat ccctcgtccg
    14641 aattggattt cggatttttt gagaaatgat ttaaaaccgt aaagtgtgag tttagaaatg
    14701 tacaattacg cttttcctta acccggaggc cagtgaaatg gacgtccgcc cagtaaatgt
    14761 aagtggatat ggaaaacttc ctgaccacta tcacgtccgc agttaaatat cagacggaat
    14821 ccgctttctt tgagcccttc cttttccgcc agaatatttg cgaccgtgac cattcgtccg
    14881 actaactttt catcggattc ctgcaagtca gttatcttgg gaatatggcg gcgcggaatt
    14941 atcaatatgt gggtgggagc tttgggacga atgtctcgaa aagctactat ttcatccgat
    15001 tcatataaaa actccgtgcc gagtttccct gcggcaattt tacagaaaat gcaatcagtt
    15061 tccattataa gcctcatttt ttaacgtcag cgtccggatt ccggcgagag ccgttacgac
    15121 tgctaactcg gtgcgcagac gatacggaaa aatatttact ttattgaaac caaaccgctg
    15181 agcttcttct atttcgtttt cttcgaatcc accttccgga ccaatcatta acgctacttg
    15241 ctcaaccgtt tgtaattgct gaatacgatt caagttggcc gaagcgtgtt gttcggcaat
    15301 cagcttgaga gggatttctt ccgtcagatt aaaccattta tccagtgtca aacaatccga
    15361 aattatgggt aaaaaagcca gtccggattg tttcaaagcc gatagtgcta atttcgccag
    15421 ccgttcgtgg ttgattttat tttttatcga atgagtggta taaagtggca taatctcagc
    15481 gacgcccagc gccgtagcgg caacaaccac atcatccatt aaattatttt tcaataagcc
    15541 aactccgaga atgatttccg gcttcaaccg cgattgaggt ggatactctt ccattatgcg
    15601 acattcagct ttattattgc taatcttttc gattcgtgct gaccagcgac gaccgttgcc
    15661 tgcgaagacg tcaatagaat cgcccctaat tttgcggagg acgcgacaac aatgtcggta
    15721 ttcatcaccc tcgaggtcga atgttccttc ctgaattttc tccattgaaa tatagaactg
    15781 ataagcgtgc attataactt tttaccaaaa ctaattttga tcaccaatcc gctgaaatcc
    15841 ttttgaccgg agtatctgtt ttctaagggg ttaccgtcct cgtcataata gacctgcggt
    15901 ggatctattt tttgagcgaa cttaagataa tcgtaaccgg cgaagatcga aacgagcgtt
    15961 ttaggcccgg aaaggaaatc aatgccacct ccgatctgga aacccggcgc ccaggctgtg
    16021 gtcattttct taatcctatc actaaaatcg ggaatattcg gcggatcgag tgccataacc
    16081 gctccaccca gacattcaag ataaggacgg aaatcattag caattttgtc cgcaaataat
    16141 attcttttat atccgcctaa taaatgcaga aatgataaac ggcgcttgct ggcactttcg
    16201 tagtaataat attgatcata atagtaatca tagtaataga gcggataatc gtcaccggtg
    16261 gcaaccgtaa acatgacctg ggcggctaag cgattggcat ttttaagcga atattcaaaa
    16321 taaccaccga ttcccgaacc ttcgttggtc tggaatatcg tgagaccaga atagcgggct
    16381 ttagcttcgg taaagttaat ttttttggcg gcagagagcg gtatagtcat tactaatcca
    16441 agcaggatta atcccacttt atatttgctt attgtgttca taatgactcc atcatttgtc
    16501 cttcaataaa caactaagaa tttacacttt gagataggaa agcccaagag attgtttttg
    16561 acccgatgcc aataggcgaa aattgagaaa ttccagtatg aaccggttta attaatatca
    16621 aggggcttgg ttgaaaaaat acaaacagct ataacagaaa aatattgttc ctctgacata
    16681 atttgaccat ttcttcaggc cagatactgc attgtatttc cccgatatga gctttccgta
    16741 aataaaacat acataaccga gattgcccga tgccaccgcc gatcgacaaa ggtaattcgc
    16801 cttttaatag acgctgatga aaataaagat tctgacgttc ttcaacctgg cgaattttca
    16861 gctggcggag aagagcttct ctatcaaccc ggattcccat cgaagacaat tcaaaagcgc
    16921 aatccaaaac tcggttgtat acgataatat cgccgtttag cccatgataa ccattgacag
    16981 tcggggttga ccaatcatcg taatccggtg cgcgcccgtc atgaggctca ccattctgta
    17041 actgtccgcc gataccgatt agaaaaatta ccccgaattt tttacaggct tccttttcac
    17101 gagcgcgggg tggcaaatcc ggatattggt ccagtaaatc ctcagaatga ataaaggtta
    17161 tcttttccgg caattcaggt gagatattcc attcatgaca aacgaatttt tcggtacgta
    17221 gaatcacgtc gtagatggta ctgacagttt ttttaagaaa atccaagttg cgctgttccg
    17281 gagtgatggc tttttcccaa tcccattgat cgacataaat tgaatgcaaa ttgtctaaaa
    17341 tgtcatcggg acgaatagca ttcatgtcgg tataaagccc tttcccgggt tgaatgccca
    17401 ggtcggtcag catcatccgc ttccatttag ctaaagactg aacgatttcg acttgggtat
    17461 ttgccatctc cttaacggtg aaggatactg gttgctcgat cccattaaga tcatcgttta
    17521 taccggtgcc ggctttgacg aacaacggtg cagttacccg gatcaaatcc agctcaaaag
    17581 ccagattctg ctgaaaaaaa tctttcagcg ctaaaatcgc cttttcggtc tccactgttg
    17641 ttaggattgt ccgataattc ttgggaataa acaatccgga attcattgat tcttctcctg
    17701 aaaaaacgct cgactttatg aataatttaa ctattatcca attctttata aaatatttct
    17761 catggcaatt aatccggtaa ttcttaaata cacggctaca aataattcta cagagattta
    17821 tttcaatttt gagatttttc aaattgccaa ttgaccagcg ggctattaaa ttccgcccaa
    17881 atggagaaaa aatatgcctg ttaatcagat tcagcttatg cgtttcaaat ccgacgagcc
    17941 ggtagatcag acaaaaaatc gtctgaaaaa attgtttaaa agtgccggtc tggatagagt
    18001 aatccaagcg ggcgacatta ccgcgataaa aattcacttc ggtgagaaag gcaacgttac
    18061 tcatgttcca gccgcctatg tcgaaccaat tgtccgaatg ataaagagtt gcggcgctcg
    18121 gacatttttg accgatacca cggtgctgta taaaagtgtg cgtcagaatg ccattgatca
    18181 tcttcagctg gcttacgata acggatttac ctatgaaaaa gtcggggcac cggttattat
    18241 cgccgacgga attatcggcc ggaacgagac cgagatcgag gtcagtgcac ctctgaacaa
    18301 aaccgtgtcg ttagcgaccg aactggtagc ggcaaattct atcattgcca ttactcacgt
    18361 caccggtcat ttgagcaccg gcctgggtgc caccatcaaa aacctcggca tggggatgtc
    18421 cagccgcaga ggtaaattag ctcaacattc cgtcagtaag cctataatta aatccaataa
    18481 atgcactgct tgtggcattt gcatgaaatg gtgtccggca aatgctattg agattcccga
    18541 caaattcgct ataattgacc aagatatttg tatcggttgc ggtgagtgtt tgaccgtctg
    18601 tcggtttgac gctgtccatt ttaaatggaa taatagtagc acgcgtttac aacaacagat
    18661 tgcggaacat gctcttggaa ttgttcggca aaaaagcggt cggattggct acctgacctt
    18721 tctaactaat atgaccaaag actgtgactg cctgcctggg aaacaagtcc cgattgcccc
    18781 cgatattggc gttctggctg gcttcgatcc cgtagcaatt gatcaagccg tcatagatct
    18841 taccaaccaa gttaataacc agaatattgc ccaattagcc catcctgaaa ttgactatag
    18901 cattcagctc gattacggtg aaaaaatcgg gctgggctgt cgcaaatatc agctaatcga
    18961 aacggaattt tagattccac taaactaatg ttttaaacat cattaaattg attaattagg
    19021 aatagagatt tttatgattg acgtattttt ctacgaagca tttcaagaag aagccgagct
    19081 gatcgagcgc tttctgcctg aaaatataac cgctggattt gattggcgaa ccattcagga
    19141 aagtgagcat cctgcaccac ccgctaaaat aattagtatc cgaactcaat caattgtgtc
    19201 agtctcctgg ttaccggaat tatcggcaat tttaagccgg agtaccggct acgatcacct
    19261 catactgtta gctcaaaaag ccggttcaag actacaggtc ggatatttgc cgctttattg
    19321 taaccgttcc gtagcagaac aggctattct cctcgctctg gcgcttctgc gcaaacttcc
    19381 ccagcaactc cagcagtttc aaaaattcca ccgcgacgga ttaactggtc gagaatgcgc
    19441 cgggaaaaca cttctggtcg ttggtgtcgg aaatatcggc catgaaattg taaaaattgg
    19501 cattggatta ggaatgcagg ttctgggagt tgatattatt aaaaaacact ccgatgttaa
    19561 ttatatcagt atcgagcagg gtttaccgaa agccgatata atcatttgtg cgatgaactt
    19621 gacttccgaa aatcataact atttcaatgc tgaccgactt cgtaaggcta aaccaggtgt
    19681 ggttttcgtc aatatcgctc gtggcgagca gtcaccttca accgaattat taaaacttat
    19741 gcaggaaggc catctcggcg gcatcggtct agatgtttat aatgttgaaa gtgaattggc
    19801 tgtcgctttg cgaagcggaa gcacaagccc taacaaagaa gtccaggcta ctcttgctct
    19861 tgctaaatta ccaaatgtca ttctgactcc tcataacgct ttcaatacct acgaatcagt
    19921 cgaacgcaaa gccgctcaga gtattactca gattcagcat ttgcttgaaa acggccgatt
    19981 tctatggcca atcccggctg atggcaagta gaagaattac cacgaaggca ctaagacact
    20041 aaaaatttac cgtatattta tcgggcttgg taaggagccg aaccgatttg tctattaccc
    20101 tggcaaggtc aagaaaaaag tagcaaacat ttggaaaatt cttttaaact agtcatcctt
    20161 ctggcggcat aacctaagat ttatttgttg acctatcaaa tttcattctt cttaaaataa
    20221 taaaaagaaa tcatagtgcc tttgagcctt tgtggtaaaa attcaaaact gctcatcgta
    20281 cttagtccag agttcatcac ccaatttcca ggcgcggttc acaactttag ccccccagtc
    20341 aatgctatat ttagttagaa aatggcgggc ggcctccggg ctatgtctat attgcctcaa
    20401 agcctgactt tcgatttcgt tctgccgtct taataattcg gattgcattg gtccccacac
    20461 agccgctaca tcatagcgca tatctcccca acgctgagcc gccagagttc ccagccgatt
    20521 aaaagcccac caggcggatt cccgattaaa gcctttcgtt cgtccgtcaa ccttatagga
    20581 tggcgccaca tcataaatac tacaatatag cggaatatat atcgaggtag cgacattatc
    20641 cagagctagc caaaccactc cgccaatctc atctggaagc cattcccgcg attgagttat
    20701 cgtagcatac atcgtatacc aacgagcgat cgggcgttca ccacgccaac cccaaccgcc
    20761 attgattttg aaaatgtgat tcatatcgta tggcataaat ggatttgcaa atggtgagat
    20821 ttcggtcttt ccttctgagt tagtccaggt gatattttta acgaaattat aatccgtacc
    20881 ctcgaagtaa tcctggaata ttctaatcat tttagccaag tctaccagag tatccggttt
    20941 aacagaaaat ggaaaatttt ccgcgtctgg atgtagtttt agagatggcg ctactaagtc
    21001 taatacacgc cattcacgcc ttcgcgccga aaacgtttga cggctttcgg gcgcataggc
    21061 ataagcaaat tcgaatggac cttctttcgg attccaccac ccgctatctt gtgccacctt
    21121 aaagacattt tcggaagcca taaaataatc gggctttttc aggtctatct gacggatgcg
    21181 actaccatta gcattcaccg aaacgtgatc atccggaacg cgttgagcta cccagattga
    21241 accacaatta ccctttcctg gtccgacgat ttccagtgcc cagacttcat ttttatcggc
    21301 aattgtcaaa cattctccct catcaatcca gccgtatttc ttagtcaatt cacccgccat
    21361 acggatcgct tcgcgagctg tcgaacaacg ttctaccatc agacgcacca attgctgaca
    21421 gtcaattaag ccgttatctg aacgtaaaca ctcgcggcca ccaaaagtag attcgccgat
    21481 tgctagctgt ttttcattca tacaagggta ggccgtattg ataaatccat aagtactgac
    21541 aacttgcggg atttcaccga tcgccagata ttgataggca ggcatcgcca aagaatcaca
    21601 atcaatcctc tttagcattg taaccatttc acccttacta tattttttgg ttggaacaat
    21661 atcgaatgac gaacgtgtgc gatgactatc gcaagtgtga ctggtcataa cggaaccatc
    21721 gcgggaggct tttttcccaa ccataactga agtacaaccg tccggatagc ctccttccca
    21781 gtcggctttt tcgcgcgcta tcccaactaa tggaataaag ctgatccaaa caactatcac
    21841 caaaatttta caaacaacag attgcttcat attaatctcc tatataattt tgaaaaagat
//
LOCUS       DAOMJU010000029        21576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2278_length_21576_cov_19.067980, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000029 DAOMJU010000000
VERSION     DAOMJU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 21576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21576
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_21576_cov_19.067980"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..170
                     /locus_tag="PLO58_04365"
     CDS             33..170
                     /locus_tag="PLO58_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74057.1"
                     /translation="MKKLLVILMVASLALVTVSCQKKTKVEEAPAPVDTTTAVTDTVN
                     A"
     gene            complement(423..1010)
                     /locus_tag="PLO58_04370"
     CDS             complement(423..1010)
                     /locus_tag="PLO58_04370"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HQO74058.1"
                     /translation="MIYQTVILAIVAYFLGSISFSYIFTKVRTGKDIRTVGVKNSGAL
                     NVFENVGKGIGLLAGIADALKAVVVVVIGKLIGLDTFPTIFAASFAVIGHCFPIYYKF
                     YGGRGASAALGILLCFIPLEVLISCIPAALIAYCIRRMGFTPVFILGFSPIVAAIFKK
                     PATIIWGVVYLVLLTGVLNLIINISKRDEKLIPEN"
     gene            complement(1023..1904)
                     /locus_tag="PLO58_04375"
     CDS             complement(1023..1904)
                     /locus_tag="PLO58_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQO74059.1"
                     /translation="MDQKILILTGDNLGLKNEQIDYPDVAVVKFPVVIDEKEYFEDET
                     HTAQYLIERFKKEKIVARSQALLRKDIVEVIEKNKDKYDLIVHVLMGSNMSSATVQMA
                     EGIRKDYEGIIPIINIDTKQASSGVGAVLLRVIELLKESDSVDEFVSRIDEVIKNTYS
                     YFCVPDLNYLYRGGRIGRAQSLLGSVLRIIPVVGLFGDETDSGFLPVGKGRTYQAANK
                     IIINHIKQKMVLKKVAKIELINILHFENSDADELKDLEKQINDNLTYRRLVHGHARFV
                     EAVHGGPGTWGGSFVLK"
     gene            complement(1916..>3640)
                     /locus_tag="PLO58_04380"
     CDS             complement(1916..>3640)
                     /locus_tag="PLO58_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HQO74060.1"
                     /translation="INDLNVFPVPDGDTGLNMVLTVQGGLAHAKNYASDQSTLSEYLT
                     ALAEGMTLNSRGCSGVILALFIQGVAKKLADWPPDQKIPNTEIAQALISGYKYAYAET
                     ANPKEGTMLTMMRVFAERFSELVMDGKDALSIIKDIVPDLEETLEKTPEMLPVLKKAG
                     VVDSGAMGFLVLIKGVATGLEYTGTRIRPIMNIATILSTSRYIRSIIKERKYESGEER
                     KKLRPLNISLNNINNISFANILTKFNNLLQRNNNHQLAEEILAQSNELDETWNPDIQY
                     RYCTEFILESDEIGKDSLKKKLESFGDSILIISSSNTYKCHIHTNKPKDLLKSLENLG
                     NISATKIDDMKVQHKNLISEDKEFYERDLAVLLIVNGEGFAKILKSLGATDILMYKQA
                     KPSVNQIGNALFKTRAKNIIVAADDSDILASLKSAITLCKSNIELIETQSVIQIISMM
                     YTFSPEMDIFSNASIMRENLDNIRFCQIAKATRQFKTDFDTIPKGNYFCLHNKSILAH
                     NSNLIDLIKLSIEKVRENESLITLYRGKMEKNNGSLIAELQQKFTQCEFEIYYGGQNR
                     YNYYITFE"
     assembly_gap    3641..3740
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4474..5748)
                     /locus_tag="PLO58_04385"
     CDS             complement(4474..5748)
                     /locus_tag="PLO58_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74061.1"
                     /translation="MKRAIFWLVILLIWFGNTAVAQITLRGFILNQDMFQTNESMDVM
                     LIRNRLRLNTETFGENVYGFASLDITNDIADSTTTLPNLREVYIDIYSNWLDLRIGKQ
                     QVVWGKTDGYFINDIVNPLDLSYFLLQDFDDIRMAMTMLNAKLHYSNHSLEVLVIPEF
                     KPMNLLFEGAWGYKRPQMMEYDSPLGKIVLPLHYQNEQIPDLSLKNAEYGLKLNTWFL
                     RTDLAFLYLRSREDKPVYFTELVNDSSSLPIRVDLTPTHPWQNFFGLNFSKPIGAFVF
                     RGEGGYYPERHFNTQDMNYLSKGMIIERPFLQFMLGGDYQLTSALDLSVQGINERILD
                     YKEGIQADENVTIGTLMLRGHFVNETVSPLILVMYNFEDYSSLTRLSIDWNYADSFTI
                     TAGVDLLAGKSDTIFGMFAKNDNVYLKFKYAF"
     gene            complement(5745..6542)
                     /locus_tag="PLO58_04390"
     CDS             complement(5745..6542)
                     /locus_tag="PLO58_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HQO74062.1"
                     /translation="MKKLFSLSIICIALVIFYSSAIAQMTGRDIMLKVEKQQDTKTRI
                     VKSKMILINKQNKTRERDVVQYVKSVNDPKGVDDKILIFFEYPADIRGTGLLMISYSD
                     VNKDDDRWLYLPALKKVRRIAGESKNEYFMGTDFTYDDMGGRSVDQDKHTLLGEETID
                     GVKCYKIESVPVDKNDIYSKKIGWVIPDKWVHLKVDFYDRKGQLMKTLKADDIRNVSG
                     VWLQFNLEMTNLAENHKTILKIIEAKINEPIDDQIFTITALERGGIR"
     gene            complement(6543..6899)
                     /locus_tag="PLO58_04395"
     CDS             complement(6543..6899)
                     /locus_tag="PLO58_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74063.1"
                     /translation="MTIEQKDKNIARGLTIISGGIVVYWLYQLILWLIRWFSTVRIET
                     SGIWSNFGGFCLENLSIILVVYVFICLQIAGVVEFRYRKNFTEAFFLGIIFTPIIMLI
                     YMKLKKGKFPDDSKEV"
     gene            complement(6896..9181)
                     /locus_tag="PLO58_04400"
     CDS             complement(6896..9181)
                     /locus_tag="PLO58_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQO74064.1"
                     /translation="MIHKIIEFDLKYPRTIIAFCILLTLLMGWNIPKIQLEPDIKALM
                     PQDFEIIENMKEMEETFGGNDLVVVSITADNIFSPATLEKIEAITSEIEMLENVDQVI
                     SITNVPDIVGTADGFEVRELIEEFPTTESEIDSLKKRIIGNRMIYGTLASSDFQKAGI
                     IAYLKVATDENSDEQVYQIFNAIKNKYAGPEQIHIAGLPLTRREVSATMAADVKKLFP
                     YGIILMIFLLVLTFHSWTGAFLPFTVVIMSIVFTVGLMVLFNIKMTIVEMMIPVMLIA
                     IANSYSIQVITQYFVEHLKNPDAEKDSLIRLILSYLTTPVLLSGFTTLVGFICLLSHV
                     LPPAKNLGILCACGTTIAFIYSLTFIPAALKLLGFPVTLKTAGSRKRTSRFLPSWGQF
                     FTRYHKPFLGLCLVAIIIVATGIGRIIVDTNPIAYWKKSSEIRQSNEVIDRNFGGSST
                     ISVLATGDIKDPQFLKKIENLCTFLESQPTVTCANSIVDMLKRMNQAFHGDSAEYYVI
                     PETREEVAQYLFLYSLTGNTEDLDRFVDYDYTQAQILARVNDSSSKASYKLYKDTQNY
                     IAENLGTENFPKVSGMAPLIGVLSELVVDGQIRSLALSIIAVFLIIAIAFRSVKAGLI
                     AIVPLIVAVLCLFGIMGHIGITLNIATALISSIMIGIGIDYTIHFLYRFRLEARNGAD
                     AEEATIRTLTTSGKGIVYNAISVIIGFMVLMLSGFEPISFFGFLIVFSIAVCLFGALT
                     ILPALLVLTKPAFIFGKRSLQ"
     gene            complement(9178..9807)
                     /locus_tag="PLO58_04405"
     CDS             complement(9178..9807)
                     /locus_tag="PLO58_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQO74065.1"
                     /translation="MKQNSFPSGEEAKTKLSPAITYARRFILANGVRGLNIDQLAADL
                     HIAKKTIYKLFPTKEQFIEYVLLDIYHELFTETRAIQLNDNEPLKCFYGIMKIMFKHI
                     SQINPQTILDVKLFYPKVWQEVEEFEHEVINRILDSFQKADNLGLLRKKFDLKFTADL
                     IVRIVQNTFQPEIFISAPYSITEMIKIFVDIIMNGLMEKQFTFNFSDDE"
     gene            10047..10436
                     /locus_tag="PLO58_04410"
     CDS             10047..10436
                     /locus_tag="PLO58_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HQO74066.1"
                     /translation="MEKIKYGYGPHLMLDLNDCDSEVLDNLDACFKLLNELPPKIGMT
                     KITQPYVFRYEGRFPGDEGITGVVIIAESHISLHTYPKKKFVFVDLFSCKSFDVETAR
                     QYIIDYFKCQSPKWYLKERGENFPQHP"
     gene            10450..11316
                     /gene="speB"
                     /locus_tag="PLO58_04415"
     CDS             10450..11316
                     /gene="speB"
                     /locus_tag="PLO58_04415"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HQO74067.1"
                     /translation="MPTPFSPGAFGALEPDYCRYEQSRIAILPIPYDGTATWQKGAAK
                     GPEALLAASANMELYDIETDSEIYRQGITTLPFLKCPKKPEDLVQKVNEKTRQLLADG
                     KFVVGIGGEHSISIGLVQAHAKHFSNLSVLQLDAHSDLRNEYERSKYNHACVMARIQE
                     ICPIMQVGIRSMDISELERLDKTRVVFAHEYRNSPEIFAKITANLTDNVYLTIDLDVF
                     DPAVFPSTGTPEPGGLGWYDVINVIAHIIQKKHIVGMDVNELLPNPSDKAPDFLAAKL
                     IYRTLSMICTKE"
     gene            11322..12341
                     /locus_tag="PLO58_04420"
     CDS             11322..12341
                     /locus_tag="PLO58_04420"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HQO74068.1"
                     /translation="MKKNDYLQKVVEHIDIKSFDARPILKAYTKMAFQARNLAVAADI
                     YDRMLKDKKCKVILTLAGSLFSAGLKNVVADMVENNMVDAIVSTGALIVDQDFFEALG
                     YKHYIGTPFSDDSELRELAIDRIYDTYIDEDELRVCDLTIAEIANSLPPRPFSSREFI
                     EEMGKYLDKVGIKTEKSVVYSAYKKGVPIFVPAFSDCSAGFGLIHHQWNRKGDMVTID
                     SARDFLELTKIKIAAKETGILMIGGGVPKNFTQDVVVATDVLGIEKPMHKYAVQLTVA
                     DERDGGLSGSTLKEASSWGKVDTKYEQMVFGEATVMMPLLVSYAYHQGNWKKRQEARF
                     SNLFV"
     gene            complement(12452..13135)
                     /locus_tag="PLO58_04425"
     CDS             complement(12452..13135)
                     /locus_tag="PLO58_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQO74069.1"
                     /translation="MENQKKAKRIINTWTGPWEKRNLPKMAAALPNWVSSDHLTILGI
                     VAALVISLGYVLTRYSNWWLMLANFGFVLHWWADSLDGTLARVRHKEREQYGYFVDHM
                     CDAFSTTIILVAFGLSPLMRLDISLFLTIGYLLLNIYAHVGAYTEGIFRLSYAKIGPT
                     EIRILVVIVNTILIFWNPTVLVLNNQPWKVVDVVGAILAGVFIIVFIIVGIKDAIKLD
                     RLDRAKWEK"
     gene            complement(13150..13566)
                     /locus_tag="PLO58_04430"
     CDS             complement(13150..13566)
                     /locus_tag="PLO58_04430"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQO74070.1"
                     /translation="MNFKKIIKFQLVAWLGTIVNLGSLWILHGILKIPVTIAGACAIE
                     LAILHNFTWYYFRTWNGRVAHTPTDFFSRLWKYNVITAGVDFVFNLGTLSVLTHFFHL
                     HYILADILGMSIGPFIKIFINELVIFKKPKLHVKNN"
     gene            complement(13931..14003)
                     /locus_tag="PLO58_04435"
     tRNA            complement(13931..14003)
                     /locus_tag="PLO58_04435"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13968..13970),aa:Met,seq:cat)
     gene            complement(14139..15590)
                     /locus_tag="PLO58_04440"
     CDS             complement(14139..15590)
                     /locus_tag="PLO58_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HQO74071.1"
                     /translation="MKQLKLDLSNLDNVFPENFTPEQIAQAKTLFFQKLSEGAHQFYG
                     GKIQIAPRAGFFGFNWFNVWYTPGVSRISTAIRDDNDLSFELTDRGNLVAIVSDSTRV
                     LGDGDVTPPGGLGVMEGKAFLMKYLGGVDAVPLCINSRDQNGLPDPDNIIQFVKMCEP
                     SFGAINLEDISQPNCYKVLDVLSEECEIPVWHDDAQGTACVTLAGLINALKLAKKEIG
                     EVKITMLGAGASNTTIARIIIAAGGDPQKIMLFDSRGGLHRNRTDIKDDSRFYRKWEL
                     CQLTNPKCVNDFAESCKGADVLIALSKPGPNVIKPEWIKSMADKAIVFACANPIPEIW
                     PHAAKEAGAFIVATGRGDFPNQVNNSLGFPGILKGALIVRARKITDSMAIAAAQALAD
                     FAEKRGITPDNIIPKMDETAVFPSEVAAVAKQAVQEGIARRIITAESAFQQAEVDIQT
                     SRAMFQTLIKESFIKELPESLLVSALEWAISQV"
     gene            complement(15612..16169)
                     /locus_tag="PLO58_04445"
     CDS             complement(15612..16169)
                     /locus_tag="PLO58_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HQO74072.1"
                     /translation="MSDICHLSTPLQNTDLIKLNIGDKVLISGKIYTARDVAHQRLVK
                     LMEIGQPLPFDIKGTVIYYAGPAPARPGNPIGSVGPTSSYRMDKFTPPLLSAGVKATI
                     GKGERSQNVIDAIIRYQAVYFVAVGGAAVVAAQAVKTARIVAFEELGPEAIYELTVVN
                     FPVFVAIDVNGNDLFRVGVARYRQE"
     gene            complement(16177..17025)
                     /locus_tag="PLO58_04450"
     CDS             complement(16177..17025)
                     /locus_tag="PLO58_04450"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HQO74073.1"
                     /translation="MKNIDVAQIIPVVKKLCIDANYYVSADVLAKAQASLQSEKSPIG
                     REIFQILIQNYELAAQWQMPLCQDTGVAVVFVELGQDVHLIGGDFEQAINEGIRQGYS
                     EGYLRKAMVSDPVVKRENTGDNTPAIIYTEIVPGSSLKITVAPKGGGSENMSEVRMMN
                     ASDGLESVKDFIVDCVRRAGGRPCPPVVVGVGLGGNFEQSALLAKKALLRPLSEKNPE
                     PFWADVEQELVERINRLGIGPQGLGGSTTTWAVSIIYKPCHIASMPVAVNLDCHCHRH
                     ETAIIE"
     gene            complement(17176..17529)
                     /locus_tag="PLO58_04455"
     CDS             complement(17176..17529)
                     /locus_tag="PLO58_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HQO74074.1"
                     /translation="MSMQLSVVLRTAVMLVFSNLFMTFAWYGHLRNLSDKAWYVAALV
                     SWGIALFEYLIQVPANRLGFTQMSLAQLKILQEAVTLTVFIPFAVFYMKQPLKTDYFY
                     AGLCILGAVYFIFRS"
     gene            complement(17560..18087)
                     /locus_tag="PLO58_04460"
     CDS             complement(17560..18087)
                     /locus_tag="PLO58_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HQO74075.1"
                     /translation="MLTIPFEKLIVKTHALWDEQNLVLASGDFKAGHFNFMTVGWGSL
                     GTMWSLPFAQVVVRPTRYTYEFMEKYETFTLCAFAAEYHKKLQIIGSTSGRRTDKVKA
                     SGITPCPARIVAAPVFQEAELQIECRKIYWSDMEPSHFLKDDIAKNYNGHDYHRIYFG
                     EIVAITGTEKYLARD"
     gene            complement(18205..19818)
                     /locus_tag="PLO58_04465"
     CDS             complement(18205..19818)
                     /locus_tag="PLO58_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HQO74076.1"
                     /translation="MAGRKYAQRNSPATALKSSFFTILIFLISLNSCSDLYQNQIPDP
                     ILPDYQGIRNMYWKIWQIMEQNIKRGNRENNFSYHYVNCESELLINQSSTIMLALFGV
                     YGSSHFPVMQSLDNFYRGQRSDGFISRIYNSMTGEYLHAPSAIEPMINPPLFTWPELK
                     YYYLTGDQSRLRKYFKIWEKYFEWLDNFCQAKGEAIGLYYNSQNGSLMINSPRTDNDF
                     GGWADMSCQMALFAEQLAETAEIIGEDTRVIYYQNRYQKITEVIREKLWHDEDGFYYD
                     LTSDGHPIRIKTTAGFWPLIAGIPSSEDAPELINHLKNDDEFARRHLFPSLSADEDGF
                     KPNGAYWRGGVWGNENYMIIAGLKRYGEYEFATTTAWNHITNMEKIFTGYFPDTSLID
                     EKYWANCNATVWELYSPENDQPGTRWDTYNLCRPNYIPTSGFGPVAMLIEDVLGFNVN
                     GPDDQLTWRLWLLNKHGIRNLKFGNNLVTLWCEKRETKESPVDIKGLTSSPFFLTIIL
                     EQDTITIKQPRGRINISFKMEDIKKINKD"
     gene            complement(19770..21065)
                     /locus_tag="PLO58_04470"
     CDS             complement(19770..21065)
                     /locus_tag="PLO58_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO74077.1"
                     /translation="MRKYVVSLLLLLFAGLAFSASDDFTKLPDGLHYRQIKKNIRPET
                     RPAFKAAEKYYEKKKYFQADQSYIETIQRGEGFYGNWGRACVARQEKRVSDAEKFYAT
                     AFTLNDSLEGFLYDYAIFLQYNSQDWNKIEKVCTKLYTVTKNDEALSILIETAARLKQ
                     QSAALKSLEKIARRYPLRANAQVYYASILYDCGQKAEAVRVARDAVQITYDPFQLKLL
                     VTILAQEGYFVESAKVCEKMSRIAPRSAQTLEAWGFLEFQQGHYENAATYYKKALNRD
                     YRIPTLLSLARIYTFYLNEPSKAMYYAKAITKLERNNCDALYIMAEAKRRAGDIHGAL
                     KYSARLIELQPEHPQTYFYHGKLLFQNKDYKNAVKYLEKAVQYNPDIKRYRLVLAKAY
                     AGAGMSEKARATYANYLNEPLKNLWQEENMLRETPPPPR"
BASE COUNT         6402 a         4675 c         4080 g         6319 t
ORIGIN      
        1 ggttttttaa aattattata aaggagagta aaatgaaaaa attgctggta attttgatgg
       61 ttgccagttt ggcgcttgtc acggtgagtt gccagaaaaa gaccaaagta gaagaggcac
      121 cggctccggt cgacacgact actgcggtta ctgatactgt taatgcgtaa tcatcgttaa
      181 cagaaatcac cgattaagtt tacacaaaat atcccgctcc ggcggggtat tttgttttta
      241 tagctaattg taagctgtat aaaattaagc caatactaga tattttattc attttgccaa
      301 tgtggtaaaa gtacatccct attagactat tttttggtag ataacaattc ttctaaacca
      361 ctcccggttc ataaaatcct ttttattaca cgtagcgaag caatctttat tgctatagaa
      421 atttaatttt ctggaattag tttctcatct ctttttgaga tattgatgat aaggttgaga
      481 accccggtta agagtacgag atatacgact ccccaaatga ttgtagctgg ttttttgaaa
      541 atagcagcta caataggaga aaagccgagg atgaacacag gagtgaagcc cattctacgg
      601 atacaataag caatgagagc cgcgggaata cagctgataa gtacttcgag cggaataaag
      661 cataaaagaa tcccaagagc ggcagaagcc cctctgccac cataaaattt gtagtagatc
      721 ggaaaacaat gcccaataac agcaaaagaa gccgcaaata tggttggaaa agtatcaagt
      781 ccaatgagtt tgccgatgac aactactacg actgctttta gcgcatccgc tattcccgcg
      841 agtaagccta ttcctttgcc cacattctca aatacattta acgctccgga atttttgacc
      901 ccaaccgttc taatgtcttt tccggtgcgt accttggtaa aaatatagct aaacgagata
      961 gatcccaaaa aataagcaac tatggcaaga attactgttt ggtaaatcat tattctaatt
     1021 tcttatttta agacaaaact cccaccccac gtacctgggc ctccatgaac agcctcaaca
     1081 aatctggcat gtccatgaac gagtcttcga taagtgaggt tatcatttat ttgcttttct
     1141 aagtccttga gctcatcggc atcactgttt tcaaaatgca gtatatttat aagctcaatt
     1201 ttagccacct tctttagaac cattttctgt ttaatatgat tgataattat cttattagcg
     1261 gcttggtatg ttcttccttt tcctacaggt aaaaatcctg aatcagtttc atcgccaaat
     1321 aaccccacta ctggtattat cctcaatact gagccgagca gagactgtgc ccttccaatt
     1381 cttccccctc tataaagata attcaagtcc ggcacacaaa aatacgagta ggtatttttt
     1441 attacttcgt ctatgcgcga gacaaattca tctacactgt ccgactcttt taggagttct
     1501 ataacccgca gaagtaccgc cccaactccg gaagaggctt gtttcgtatc aatattaata
     1561 attgggataa tgccttcata gtctttgcgg attccttctg ccatctggac tgttgccgaa
     1621 gacatattac ttcccattaa cacatgaaca atcagatcat atttatcctt gttcttttca
     1681 attacctcaa cgatgtcttt tctcagcaat gcctgagagc gagccacgat tttctctttc
     1741 ttgaaacgct caatgagata ttgagcagtg tgtgtttcgt cttcaaaata ttctttttca
     1801 tcaattacca caggaaattt tactaccgca acgtccgggt aatcaatttg ctcatttttt
     1861 aatccgagat tatcgcctgt cagtataagt attttctgat ccatttcgat tctccttatt
     1921 caaaggtaat ataatagttg tagcgatttt ggccaccata atatatttca aactcgcatt
     1981 gcgtgaattt ttgttgcaac tctgcaatca gcgatccgtt gtttttttcc attttgcctc
     2041 tgtataaggt aataagcgat tcattttccc tcactttttc tatggaaagt ttgataagat
     2101 caatcaagtt tgaattatgg gcaaggatgc ttttattatg taggcagaaa tagttgcctt
     2161 taggtattgt atcaaaatcg gttttaaact gccgcgttgc ttttgctatt tggcaaaacc
     2221 taatattatc gaggttttcc cgcatgatac tggcatttga aaaaatatcc atttcaggtg
     2281 aaaatgtgta catcatactg attatctgaa tcacactctg ggtttctata agttctatat
     2341 tacttttgca tagcgtaatg gcagatttga gactggcgag gatatctgag tcatcagccg
     2401 caacaatgat gttttttgcc ctggttttga ataaggcatt ccctatttgg ttaacggaag
     2461 gttttgcttg cttatacata agaatatcgg ttgctccgag gcttttcaag attttagcaa
     2521 atccctctcc attgacaata agtagcaccg ccagatcgcg ttcatagaat tctttatctt
     2581 ccgatataag gtttttatgc tgaaccttca tatcgtctat tttagtcgct gagatattcc
     2641 caaggttttc taaagattta agaaggtctt taggtttgtt agtatgaata tggcacttat
     2701 aagtattgct agaagaaata atgaggatgc tatctccgaa tgattccagt ttctttttta
     2761 gtgaatcttt tcctatttca tctgattcga ggatgaattc agtgcaatac cgatattgta
     2821 tatccgggtt ccaggtttca tccagttcat tactttgcgc taaaatctct tcggccagtt
     2881 gatggttatt attgcgctgg agcaaattat taaacttggt cagaatattt gcgaaggaaa
     2941 tattgttaat attatttagg gaaatattca atggtcgtaa ttttttccgt tcttctccag
     3001 actcatattt tcgctctttt ataattgaac gaatataacg gcttgtcgag agaatggtgg
     3061 caatattcat aatcggacga atccttgtac ccgtatattc cagtcccgta gctactcctt
     3121 tgataagcac cagaaatccc atagcaccgg aatccaccac acctgctttt ttaagaacgg
     3181 ggagcatttc aggagtcttc tccaacgttt cttccagatc ggggacaata tctttgataa
     3241 tagaaagggc atccttgcca tccattacca gttcagaaaa ccgctctgca aatactctca
     3301 tcatagtgag catcgtgcct tctttcgggt tggcagtctc ggcataggcg tatttatatc
     3361 ccgaaataag tgcttgcgca atctccgtgt taggtatttt ctgatctgga ggccagtctg
     3421 ctagtttttt cgccacccct tggataaata gagccagaat aacacccgaa cacccgcgac
     3481 tatttagtgt cattccttcg gcaagggcgg ttagatattc cgataaagtt gattggtcgg
     3541 aggcataatt tttggcatga gccaagccac cctgcaccgt aagaaccata ttaagacccg
     3601 tatcaccgtc gggaacggga aaaacattca ggtcgttaat nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn ccatattaag acccgtatca ccgtcgggaa cgggaaaaac
     3781 attcaggtcg ttaatccgat tcttttcaat agagagcact gtcagtgcgt cttttagaat
     3841 gctattgaga actgaagccg ttatttccat ttccactctc tcaaaatttc accacacttc
     3901 aacatttagg gattcaaata agcaatatag tttattttac ataatagctt tgattgatgc
     3961 aaattcattt ttctatattg gtgtaaattt taaggatgtg gtatccactt tttagaaatc
     4021 cagattaatg gaaaatagct ttcggtaagg cgaaagatgc tttagtgtaa ccaaacacgc
     4081 caaagttcat taaaaatttt tcgccataat gacgcaaatc aaaaggaaaa gcggattcgg
     4141 cctattcgtc gtcgtcaatc ataattgcga tcaatcaaaa taaaagctaa acgttgccct
     4201 tgttccagtg cgattactat tcaccgattt atacctgtta gcttttgata aatcgaggta
     4261 agtaacggac ggaccggctg agtaaaatac ttccgtgaca gccctaacta acattcatta
     4321 agatgagtaa atttcttaaa aagattgagg ttcgctgaaa tcttctcaac taactaaaac
     4381 ttcttcccac tatattctgg aaaaaaacac tcctcctatg cctataagag atatttttta
     4441 atttaaaata ttggctgaaa agtatgcttt taattaaaaa gcgtacttaa acttcagata
     4501 gacattatcg tttttagcaa acatcccaaa aatcgtgtca gattttcccg ctagtaaatc
     4561 tactcccgcc gtgatcgtaa agctatccgc ataattccaa tcaatactca agcgcgtcaa
     4621 tgaagagtaa tcctcgaaat tatacatcac taaaattagc ggtgaaacag tttcattaac
     4681 aaaatgacct ctcagcatca acgtaccaat agtgacattt tcatcggctt gaataccctc
     4741 tttataatcc agaattcgtt cgttaattcc ttgaacagat aaatccaaag ccgatgttaa
     4801 ctgataatcg ccaccaagca taaattgtaa aaaaggcctc tcgattatca ttccttttga
     4861 tagatagttc atatcttgag tgttgaaatg gcgctcggga taatagccgc cttctccacg
     4921 gaacacaaat gccccgattg gcttggagaa attcaatccg aagaaattct gccagggatg
     4981 cgtcggtgtc aaatctaccc gaattggtaa actcgaagag tcattaacaa gttcggtaaa
     5041 atacacaggc ttatcttcac gactacgcag atataaaaat gccaggtcgg ttctcaaaaa
     5101 ccaggtattt aatttcaaac catattcagc attttttaac gacaggtcag gaatttgttc
     5161 attctgataa tgcaaaggaa gtacaatttt gcccagcgga ctgtcgtact ccatcatttg
     5221 tggtctttta taaccccaag caccctcaaa cagcagattc atcggcttga attccgggat
     5281 tacgagcact tccaacgagt gattactgta gtgcaatttg gcattcagca tagtcattgc
     5341 catgcgaata tcgtcaaaat cctgtaggag aaaataggat aaatccagcg gattgacgat
     5401 gtcgttgata aaatagccgt cggttttacc ccagaccacc tgctgtttgc cgattctaag
     5461 gtcgagccaa ttgctataaa tgtcaatgta cacttcccgt aaattcggca acgtcgtcgt
     5521 gctgtcggct atgtcgttgg taatatcaag cgacgcaaaa ccataaacat tttcaccaaa
     5581 agtttcggtg ttcaatctca gacgattgcg aatcaacata acatccattg attcattagt
     5641 ttgaaacata tcctgattga gaataaaacc tcgcagagta atttgcgcta cggctgtatt
     5701 gccaaaccag attaacaaaa ttaccagcca gaagattgcc cgtttcatcg gatgcctccg
     5761 cgttcgaggg ctgtaatcgt aaatatttgg tcatcaatag gttcgttgat tttagcctcg
     5821 ataattttga gaatggtttt gtgattttca gccaaattgg tcatttcaag gttaaactgc
     5881 aaccagacgc cggagacatt tcgaatatca tcggctttga gggttttcat cagttgacct
     5941 ttccggtcat agaaatctac ctttaggtgc acccatttgt ccggaatcac ccagccaatt
     6001 tttttactgt aaatgtcatt tttgtctacc ggaaccgact cgattttgta acacttcacg
     6061 ccatcaatcg tctcttcgcc gagtagagtg tgtttatcct gatcaacgct tcgcccaccc
     6121 atatcatcat aagtaaaatc cgtacccatg aaatattcat tcttgctttc accagcgatg
     6181 cgccggactt tttttaatgc gggcaggtaa agccagcggt catcatcttt attgacatcc
     6241 gagtaggaaa tcatcagtaa tcccgtgcca cgaatgtcgg ctgggtattc gaaaaatatt
     6301 agaattttat catcaacgcc tttgggatca ttgaccgatt taacgtattg gacgacatcc
     6361 cgctcacggg tcttattttg cttattgatc aagatcattt tcgacttaac aatccgcgtc
     6421 ttagtatcct gctgtttctc gactttcagc ataatgtcgc gcccggtcat ttgggctata
     6481 gcggaactat aaaaaatcac aagggcgatg caaattatac ttagagaaaa aagctttttc
     6541 atctaaacct cctttgaatc atcaggaaat ttcccttttt ttaatttcat ataaatcaac
     6601 ataattatcg gagtaaatat tattcctagg aaaaaagcct ccgtaaaatt tttccgataa
     6661 cgaaattcaa ccacaccagc aatttgcaaa cagataaata cataaactac tagaataatg
     6721 ctcagatttt ccaggcaaaa cccgccgaaa ttcgaccata tcccagacgt ttcaatccta
     6781 acagtactga accaccgaat taaccagaga atcagctgat atagccaata gactacaatg
     6841 ccaccagaaa ttatcgttag cccgcgcgct atatttttat ctttttgttc tattgtcact
     6901 gtaaactcct tttgccgaat ataaaagccg gtttggtcag gactaataaa gccggtaaaa
     6961 tcgtcaaggc gccaaaaaga caaacggcaa ttgaaaacac aatgagaaat ccgaaaaagc
     7021 tgatcggctc aaatccggat aacatcagga ccataaaacc aataataacc gagatggcgt
     7081 tataaacaat tccctttcca ctggttgtaa gtgtgcgaat agtagcctct tcagcgtcag
     7141 cgccgttgcg cgcttccagt cgaaaacgat ataaaaagtg aattgtgtaa tcaatcccaa
     7201 ttccaatcat aatactggaa attaaagccg tcgcaatatt aagagtaata ccgatatgtc
     7261 ccataatccc gaacaaacac aaaaccgcca caattagcgg cacgatagcg atcaatcccg
     7321 cttttacgga gcgaaaggct atcgcaataa ttagaaaaac agcgataatc gaaagtgcca
     7381 aacttcggat ttggccatca acaaccaact ccgataagac tccgataagc ggcgccattc
     7441 cagaaacttt agggaaattt tctgtcccta aattttcagc gatataattc tgcgtatctt
     7501 tatacaattt atatgaggct ttcgaactgg agtcgttcac tcgtgccagg atttgcgcct
     7561 gggtataatc gtaatccaca aagcgatcca gatcctcggt attgccagtc aatgaataga
     7621 gaaaaagata ctgcgctact tcctctctgg tctccggaat aacataatac tcggcactgt
     7681 cgccgtgaaa agcctgattc atccgcttaa gcatatccac tatcgaattg gcgcaggtga
     7741 cggtcggctg actctccaga aaggtacaaa ggttttctat cttctttaaa aattgagggt
     7801 cttttatatc gccagtagca agtactgata tagttgaact gccaccgaag tttcggtcaa
     7861 ttacttcatt gctttggcga atttcagaag attttttcca ataggcaatt ggattagtat
     7921 caacgattat acgtccgatt ccggttgcga cgataataat tgccaccaag caaagtccca
     7981 gaaatggctt atgataacga gtgaaaaatt gcccccagct cggcagaaac cgactggttc
     8041 gcttacgaga accggcggtt ttcaaggtta ccggaaaacc aagcaatttt aacgccgccg
     8101 gaatgaaagt caggctataa ataaaggcaa tagtcgttcc gcaggcacat aatatcccga
     8161 gattttttgc cggtggtaaa acgtgtgata gcaggcaaat aaatccaacc agcgtcgtaa
     8221 atccggaaag cagaactggc gtcgttaaat atgacaggat caatcggatt aaactgtctt
     8281 tttcagcatc cggatttttg aggtgctcga caaaatattg ggtaataacc tgaatactat
     8341 aactattagc aatggcgatg agcattaccg gaatcatcat ctcgacgatt gtcattttaa
     8401 tattgaacaa aaccattaaa ccgacagtaa atacaatact cataattacg acggtaaacg
     8461 gtagaaatgc tccagtccaa ctgtgaaaag tcaacaccag caggaaaatc atcaaaataa
     8521 taccatacgg aaagagtttc ttgacgtccg cggccatcgt cgccgatacc tctcgacgag
     8581 tcaaaggcaa tccagcaatg tgaatctgct ccggtccggc atatttattt ttaatagcgt
     8641 tgaaaatctg ataaacctgc tcgtcactat tttcatcagt ggcaactttc aagtaggcaa
     8701 tgattcccgc cttctgaaaa tcagacgaag ccaaagtccc ataaatcatt cgatttccaa
     8761 taatgcgctt cttcagcgaa tctatctccg actcggtggt tggaaattcc tcgattagtt
     8821 ctcgcacctc aaatccgtcg gcagtgccaa ctatatccgg aacattagta atcgaaatga
     8881 cttgatcaac gttttccagc atttcaattt ccgaagttat cgcctcaatt ttttccagcg
     8941 ttgctggaga aaagatatta tccgcagtaa ttgaaaccac aactaaatca ttgccgccga
     9001 aagtttcttc catctccttc atattttcga taatctcaaa atcctgcggc ataagggctt
     9061 taatatccgg ttcgagctgg atcttgggaa tattccagcc catcaataga gttaatagaa
     9121 tacagaaagc aatgatcgtt cgcggatatt tcaggtcaaa ttcaattatt ttgtgaatca
     9181 ttcatcatcc gaaaaattaa aggtgaattg tttctccatc agtccgttca taattatatc
     9241 tacaaatatt tttatcatct cggtaatcga atagggcgca ctaatgaata tctccggctg
     9301 aaaggtgttc tgaacaattc ttacgattaa atcggctgtg aatttgaggt caaatttctt
     9361 gcgcaatagt ccgagattat ctgccttttg aaaagaatcc agaattcgat tgattacctc
     9421 atgttcgaat tcttctactt cctgccacac tttgggataa aacaatttta catcgagaat
     9481 tgtttgcgga ttaatttgag agatatgctt gaacattatt ttcattattc cataaaaaca
     9541 cttaagcggc tcattatcat ttagctggat agcacgggtc tcggtaaaca attcatgata
     9601 aatgtcgaga agaacatact cgatgaattg ttctttggtt gggaacagtt tgtaaatggt
     9661 ctttttagcg atgtggaggt cggcggctaa ttggtcaata tttaagccgc ggacgccatt
     9721 ggcgagaatg aagcggcgag cgtaggtaat tgccggagag agtttagttt tggcttcttc
     9781 acccgatgga aacgaatttt gtttcatacg tttccaatct aaaatatttt aaaattaaag
     9841 tcaatagcta attttgcaat gaatcaattt aagattaaaa actttctccg tcgtaaatca
     9901 tctaattttt tacctaataa tttttatttg aaatattaaa attttaatgt ataatagcgc
     9961 gtttttttac agatttcaaa acgatgttga tcgtcggatt gaaattcgtt gtcgtaagta
    10021 actcattaat ggagggttta tgcgaaatgg aaaaaatcaa atacggctac ggacctcact
    10081 taatgctgga tttgaatgat tgcgattcag aagtattaga taatctcgat gcttgtttca
    10141 agctattaaa cgaactcccc cctaaaatcg gcatgaccaa aattactcaa ccttacgttt
    10201 tccgttatga agggcgtttt cccggtgacg aaggtataac gggcgtcgtg atcatcgccg
    10261 aaagccacat ttctttacat acttatccca agaaaaaatt cgttttcgtc gatctgttct
    10321 cctgcaaatc attcgatgtt gagacggctc gccaatacat cattgattat tttaaatgcc
    10381 agtcgccgaa atggtaccta aaggaacgcg gcgaaaattt cccccaacat ccttagttta
    10441 acctaaacta tgccgactcc cttttcacct ggagcatttg gtgcgctcga gccggattat
    10501 tgccgatacg agcaatcccg gatagccatt ctaccaattc cttacgacgg aacagccact
    10561 tggcaaaaag gggccgctaa aggtcctgaa gcattattgg ccgcctcggc taatatggaa
    10621 ctttacgata ttgagaccga ttcggagatt tatcggcaag gcatcactac gctacccttc
    10681 ctcaaatgcc ccaaaaagcc ggaggattta gtccaaaaag tcaacgaaaa aaccaggcag
    10741 cttctcgccg atggaaaatt tgtagtcggt atcggcggtg aacactcgat ttcgatcgga
    10801 ctggtacagg ctcacgccaa gcacttctct aacttgtcag tcttgcagct ggatgcccac
    10861 tccgatttgc ggaacgaata tgagcgtagt aaatacaatc acgcttgcgt aatggcacgt
    10921 attcaagaaa tctgcccaat catgcaggtc ggcattcgta gtatggatat tagcgaatta
    10981 gagcggttag ataaaacacg agtggttttt gcgcatgaat accgcaattc accggagatt
    11041 ttcgcgaaaa tcactgccaa tttgaccgac aacgtttatc tgaccattga tctggatgtt
    11101 ttcgatccgg cggttttccc ctctaccggt actcctgaac cgggtggact gggctggtac
    11161 gatgttatca acgtcattgc gcatattatt caaaaaaagc acatcgtcgg tatggacgtt
    11221 aatgagcttt tgcctaaccc atctgacaaa gcccctgatt ttctagcggc taaactcatc
    11281 tatcgaacct taagtatgat ttgtacaaag gagtaaatga aatgaaaaaa aacgactatc
    11341 tgcaaaaggt ggttgaacat attgatatta aatcattcga cgcgcgtccg attctgaaag
    11401 cctatactaa aatggctttt caggcacgca atttagctgt cgcggctgat atttatgacc
    11461 gaatgctaaa agacaaaaaa tgtaaagtca tcctgactct agccgggtca ttattcagtg
    11521 ccggattgaa aaacgtcgta gctgatatgg tcgaaaataa tatggttgat gccatcgttt
    11581 caaccggagc tttgatcgtt gatcaggatt tcttcgaagc actcggttat aaacattata
    11641 tcggaactcc ttttagtgac gatagcgaat tacgtgaatt ggctattgac cgcatttatg
    11701 acacttatat cgatgaagat gaactgcggg tctgcgatct gaccatcgcc gaaatcgcta
    11761 actctctgcc accgcggcct ttttcttcgc gcgagtttat cgaggaaatg ggaaagtatc
    11821 tcgataaagt tggtatcaaa accgaaaaat cggtcgtcta ttcagcttat aaaaaaggtg
    11881 ttccgatctt cgtccctgcg ttttcggatt gttcggctgg tttcgggcta attcaccatc
    11941 aatggaatcg caaaggcgat atggtaacga ttgattccgc tcgtgatttc ctcgaactga
    12001 ctaaaatcaa aatcgccgct aaagaaaccg gcattctaat gattggcggt ggtgttccca
    12061 aaaattttac tcaggacgtt gttgtagcca cagacgtgct cggaatcgaa aaaccaatgc
    12121 ataaatatgc tgttcagtta accgtggctg acgaacgcga tggtgggcta tctggatcaa
    12181 ccctcaaaga agccagttcc tggggcaaag tcgacactaa gtacgaacaa atggttttcg
    12241 gcgaggctac ggtgatgatg ccgctcttgg tcagttatgc ttatcatcag ggtaattgga
    12301 aaaaacgcca ggaagcccgc ttcagtaatc tttttgttta gtaatcttaa cccaaacagt
    12361 cgttatactg aaattatagt aagggagtaa aaggttaatt ggtcatcttt cccctaccaa
    12421 aaataataga ttaattacac tagttgcttg attatttttc ccatttagca cgatcgaggc
    12481 ggtctaattt aattgcgtct ttgatgccaa caattataaa aactataatg aaaaccccag
    12541 ccagaatcgc accgacaacg tcaactactt tccacggttg attatttaac accagcacag
    12601 taggattcca gaaaatcagt atcgtattta caattaccac cagaatacga atttcggtcg
    12661 gaccgatctt ggcgtatgat aagcgaaaaa taccttcggt ataagcgccg acgtgggcat
    12721 aaatattcag caacaaataa ccgatcgtga gaaacagact aatgtctaaa cgcatcagcg
    12781 gtgaaagtcc aaacgcaact agaataatcg tcgtcgaaaa agcgtcgcac atatgatcaa
    12841 cgaaataccc gtattgttcc cgctctttat ggcgcacccg cgccaatgtt ccatcgaggc
    12901 tgtctgccca ccaatgtaaa acaaagccaa agtttgccaa cattaaccac caattcgaat
    12961 atctggttag cacatatcca agcgaaatta ctaaagccgc cacgatacct aaaattgtaa
    13021 gatggtcgga agaaacccag ttcggtagag cagccgccat cttaggaaga ttgcgttttt
    13081 cccagggacc agtccaggta ttaatgattc gtttggcttt cttttgattt tccattatgt
    13141 ctttttcccc taattatttt tcacatgcaa tttaggcttc ttaaaaataa ccagttcatt
    13201 aataaatatt ttaataaatg gacctataga catccccaaa atatcagcta aaatataatg
    13261 aagatgaaaa aaatgggtaa gcaccgataa tgtacccaaa ttgaaaacaa aatccactcc
    13321 ggctgtaata acattgtatt tccagagtct tgagaaaaaa tcagtgggag tgtgggcaac
    13381 tctaccattc caggtgcgga aataatacca ggtaaaatta tgcaaaatag ctaattcaat
    13441 tgcgcaggct ccggcaatcg tcaccgggat ttttaatata ccgtgcaata tccaaagtga
    13501 accaagatta acaatcgttc ccagccaggc tacgagttga aatttgataa ttttcttgaa
    13561 attcatttaa ttagttacgt tataaatcaa atagtcaaaa tgctcataca ggcgcaaata
    13621 taaaatattt attatagttt tcaaactcta atatctttgc tggaaatcct taataaattc
    13681 gaagtccata ttttctaata atcaatacct tgcgccttgc gccagcgttt gattagactt
    13741 tgagtaactt tgtagcgggg actggattcg aaccagtgtc ccgatccgtc gggatatgag
    13801 cccaacgagt tccaacgagc tactttacag gtaattttac ttaaggaaga acattcaaaa
    13861 agattataca gttttaaaat aaaaactttg cgctctttgc gagagattaa atggacttta
    13921 aattaatttg tagcgggggt aggattcgaa cctacgacct ttgggttatg agcccaacga
    13981 gctaccaact gctccacccc gcgatcaatt ttaaaaatcg ggcataattt gcataaaatt
    14041 tggcacaata acaataatca aaaattgttt tattcaaaca aaatcacaga attccatttt
    14101 gctaaaaatc ggattgcttt taatcggctc cctgccaatt agacctggct aatcgcccat
    14161 tcgagggctg aaaccaacaa actctcagga agctctttga taaaactttc ttttatcagg
    14221 gtttgaaaca tcgcccgaga ggtttgaata tctacttcgg cttgttgaaa tgccgactca
    14281 gcagtaatta ttcgccgggc aattccctct tgaactgctt gcttagctac cgccgcgact
    14341 tcagatggaa aaactgcggt ttcatccatt ttcggaataa tattatccgg tgttatgcca
    14401 cgcttttcgg caaaatcagc caaagcttgt gccgccgcaa ttgccatcga atcggttatt
    14461 ttacgagctc ggactatcag agcccctttt aaaataccag gaaagcccag tgaattatta
    14521 acttgattgg gaaaatcgcc acgaccagta gccacaatga aagcacccgc ttctttggcg
    14581 gcgtgcggcc aaatttccgg aatgggatta gcgcaagcga aaacaattgc tttatcagcc
    14641 atagatttga tccattccgg cttaatgaca ttcggtcccg gttttgagag tgcaatcaac
    14701 acatccgccc ctttacatga ttcggcaaaa tcattgacgc attttggatt ggttaattgg
    14761 cataattccc atttacgata aaaccgcgaa tcgtctttaa tatccgtacg attgcgatgg
    14821 agccctcctc gggaatcaaa gagcataatc ttttggggat cgcctccagc cgcaataata
    14881 attctggcta tagttgtatt cgaagcacca gcccctaaca ttgtgatttt cacttcgccg
    14941 atctccttct ttgccaattt caaggcatta attagaccgg ctaaggtcac acaagctgtc
    15001 ccttgagcat cgtcatgcca aactggaatt tcacattcct cgctgagcac atccaaaacc
    15061 ttataacagt tcggctgaga aatatcttct agattgatag ctccaaaact cggttcacac
    15121 atcttgacaa attggatgat attatcggga tcgggtaagc cgttctgatc gcgactatta
    15181 atacagagcg gtacggcatc tactccgcca agatatttca ttaaaaaggc tttgccctcc
    15241 ataactccca gacctcccgg tggagtcaca tctccatcac caagcacccg agtcgaatcg
    15301 ctgacaatcg ctactaaatt tccgcgatca gtcaattcaa aagagagatc attgtcatct
    15361 cgaattgcag tcgaaatgcg cgagaccccc ggtgtgtacc agacattaaa ccagttaaat
    15421 ccaaaaaatc ccgcccgcgg cgctatttga attttacctc cataaaattg atgagcacct
    15481 tctgacaact tctgaaaaaa caatgtcttt gcttgagcga tttgttctgg cgtaaaattc
    15541 tccggaaaaa cgttatcaag attgctgaga tcgagtttca attgtttcat aaatattatc
    15601 tcctgtaaaa tttattcctg acgataacga gcaactccga cgcggaaaag atcattaccg
    15661 tttacatcaa tagccacaaa aactggaaaa ttaactaccg taagttcgta gatagcttct
    15721 ggaccaagtt cctcaaaagc aacgatacga gccgttttta cggcttgtgc cgccacgact
    15781 gccgcgcccc ccacagcgac gaagtaaacc gcctggtagc gaataatggc atcaatcacg
    15841 ttctgactgc gttcgccttt accaatcgta gccttcactc cggctgacag tagcggcgga
    15901 gtgaatttat ccattcgata cgaactggtt ggaccgaccg atccaatcgg attaccgggc
    15961 cgagccggcg ccggtccggc ataataaatc accgtcccct tgatatcaaa cggcagaggc
    16021 tggccaattt ccataagttt caccaaacgt tgatgagcaa catcgcgggc ggtataaatt
    16081 tttcccgata ttaaaacttt atcaccgata ttcagtttta tcaaatctgt gttttgaagg
    16141 ggagtggata aatggcaaat atctgacatg ttgattttac tcaattatag cggtttcatg
    16201 acgatgacaa tgacaatcga gattgactgc cactggcata gaagctatat gacagggttt
    16261 ataaatgatg ctaaccgccc aggtagtcgt acttccaccc aatccctgtg gcccaattcc
    16321 cagccgattg attctttcca ccaattcctg ttcgacatca gcccaaaatg gctcaggatt
    16381 tttctcgctt aatggtcgta ataaagcttt tttggctaat aaagccgact gctcaaaatt
    16441 gccacccagt ccaactccaa cgacaactgg cggacatggc cgcccaccgg cgcgacgtac
    16501 acaatccaca ataaaatcct tgacactttc caaaccatcg gaagcgttca tcatccgcac
    16561 ttcggacata ttttcggagc caccgccttt gggcgcgacg gtaattttca aagaagaacc
    16621 aggcacaatt tcggtataaa taatcgccgg agtgttatcc ccggtatttt ctcgtttgac
    16681 aaccggatcg ctaaccatcg ccttgcgcaa ataaccttct gaatatcctt gccgtatccc
    16741 ttcattaatc gcctgctcga aatcgcctcc aattagatga acatcctgac caagttcgac
    16801 aaatactaca gccactccgg tatcctgaca aagcggcatc tgccattgcg cggccagttc
    16861 gtaattttgg attaatatct ggaaaatttc acgaccgatc ggcgatttct cactttggag
    16921 cgaagcctgg gctttcgcca atacatcggc ggaaacgtaa taattggcgt caatgcataa
    16981 ctttttaacg acggggatta tctgggcgac atcaatattt ttcacaggat atttccactt
    17041 atccaacggt tggatataat tttcaaagcg caaaataaaa gtaatttatt catcagataa
    17101 aacgtaaata acatccttca tatatctaat atccaaatca caatttacat aaaatctagg
    17161 caaaatcttt tctccttacg aacggaaaat aaaatacacc gcacccaaaa tacagagacc
    17221 agcataaaaa tagtcggttt tcagtggctg tttcatataa aaaactgcaa aggggataaa
    17281 gaccgtgagc gtcacggctt cttgcaaaat tttcaattgc gccagcgaca tttgggtaaa
    17341 gcccaaacga ttggcgggaa cttgaatcaa atattcaaac aacgcaattc cccagctgac
    17401 caaagcggct acataccagg ctttgtcact taagttacga agatggccat accaggcaaa
    17461 agtcataaac aaattcgaaa ataccagcat cacagcggtt cgcaaaacga ctgataactg
    17521 cattgacata cctcagttct attcttttct ttatttaatt taatctctag ctaagtactt
    17581 ctcagttccg gtgattgcga cgatttcgcc gaaataaatt cgatggtaat cgtgtccatt
    17641 ataattttta gcaatatcgt ccttgagaaa atggctgggc tccatatcac tccagtatat
    17701 tttgcgacac tcgatttgca attcggcttc ctgaaaaacc ggcgccgcca cgatcctcgc
    17761 cggacaaggc gtaataccgc ttgctttgac tttgtcagtc cggcgaccag aagtcgaacc
    17821 gattatttgt aattttttgt ggtactcagc cgcaaaagcg caaagtgtaa aggtttcata
    17881 cttttccata aactcataag tataacgggt cggacggacg acaacctgcg caaatggtag
    17941 agaccacatt gttcccagac tgccccaacc taccgtcata aaattaaagt gtccggcttt
    18001 gaaatcacca ctggcgagta ctagattttg ttcatcccag agggcatgag tttttacaat
    18061 taatttttca aacggaatcg ttaacatttt cttggtctcc tttgagtttt ctgtcattat
    18121 tctaatggtg aaagtttata aattcttaaa aactgagggc tcggcatttt caattcatct
    18181 tattgttatc gttaaataat ggaattagtc cttgttaatc ttcttaatat cttccatttt
    18241 aaagctgatg ttaattcggc cgcgcggttg tttgatagta atagtatctt gttcgagaat
    18301 tattgttagg aaaaacggtg aacttgttaa gcctttaata tcaaccggag actcttttgt
    18361 ctcgcgtttc tcgcaccaga gcgtaaccag attattcccg aattttaaat tgcgaatgcc
    18421 atgtttattt aacagccaca agcgccaggt cagttggtcg tccggtccat ttacgttaaa
    18481 tcccagtaca tcttcgatca gcatagcgac cggtccaaat ccggaagtcg gaatataatt
    18541 tgggcgacag agattataag tatcccagcg cgtaccgggt tgatcgtttt ccggtgaata
    18601 taattcccac actgtggcat tacagttcgc ccagtatttt tcatcaatca aacttgtatc
    18661 aggaaaatat ccagtgaaaa ttttttccat atttgtgata tgattccagg cggtagtagt
    18721 ggcaaattca tattcgccat accttttcaa tcccgcgata atcatataat tttcattgcc
    18781 ccagacgcca ccccgccagt aagcaccatt aggtttgaag ccatcttcat cagcagataa
    18841 tgacggaaag agatgccgcc gggcaaattc atcatcattt ttcaaatgat taattagttc
    18901 aggagcgtct tcgctggatg gaatgccggc aattagcggc caaaatccag cggtcgtctt
    18961 tattctaatt gggtgaccgt cactggtcaa atcataataa aatccatcct catcgtgcca
    19021 gagtttctcg cgaatgactt cggtgatctt ttgatagcga ttttgataat aaatcactcg
    19081 ggtatcttcc ccgatgattt cagcagtttc ggctaattgt tctgcaaaaa gagccatttg
    19141 gcacgacata tctgcccatc ccccaaaatc attatcggtg cgtggagaat tgatcatcag
    19201 agaaccattc tggctgttat aatatagccc gatcgcttcg cccttagcct gacagaaatt
    19261 atccagccat tcaaaatatt tttcccagat cttaaaatac tttcgcaacc gactttgatc
    19321 accagtcagg taataatatt tcaattctgg ccaggtgaaa agaggcggat taatcatcgg
    19381 ttctatagca ctgggagcat gcaaatactc accggtcatc gagttgtata tccgcgaaat
    19441 aaatccatct gaacgttggc cacggtaaaa attatcaagg gactgcatta ccggaaaatg
    19501 ggaactacca taaacaccaa aaagagccag cataatcgtt gaactttgat tgattagcaa
    19561 ctcactttca caattgacat aatgataact gaagttattt tcacgattgc cacgttttat
    19621 attttgctcc attatttgcc agattttcca atacatattc ctgatgccct ggtaatccgg
    19681 aaggattgga tccggtatct ggttttggta tagatcggaa caactattta gactgattaa
    19741 aaaaatcaga atagtaaaga aactcgattt cagcgcggtg gcgggggagt ttctctgagc
    19801 atattttctt cctgccataa gttctttagt ggttcattta aataattagc gtaggttgcg
    19861 cgggcttttt cagacatacc ggctccggca taagctttag ctaacactaa acgataacgt
    19921 ttaatgtctg gattatattg aacagccttt tctaaatatt tgacagcatt cttataatct
    19981 ttattttgaa ataataactt tccatgataa aaataagtct gcggatgttc aggctgaagt
    20041 tcaatcaaac gagctgaata cttcaaggct ccgtgaatgt cacctgctct tcgtttggct
    20101 tccgccatta tataaagcgc atcacaatta ttgcgctcca gtttcgtaat cgcttttgca
    20161 taatacattg ccttgctcgg ttcattcagg taaaaagtat atattcgagc tagcgataat
    20221 agtgtgggaa ttcggtaatc ccgatttagt gcttttttat aataagtagc cgcgttttca
    20281 taatgcccct gttgaaattc aaggaatccc caggcttcta atgtctgagc tgaacgtggt
    20341 gctattctcg acatcttttc gcagactttg gcgctttcga cgaaatagcc ttcctgggct
    20401 aaaatcgtaa ctaatagttt aagctgaaaa gggtcatagg taatctggac ggcatcgcgc
    20461 gccactcgga cagcttcagc cttttgtcca cagtcatata atatcgaagc gtaatatact
    20521 tgagcattag ctcgtaaagg atagcgtctg gctattttct ccaaactttt caaagcggct
    20581 gactgttgtt ttaaccgagc ggcggtttcg attaaaatgg acaaagcttc gtcattttta
    20641 gtcacggtgt agagtttggt gcaaaccttt tcaattttgt tccaatcctg actattgtat
    20701 tgcagaaaaa tggcgtaatc gtatagaaag ccttctaagg aatcgttgag agtaaaagcc
    20761 gtggcataga atttttcggc atctgagact ctcttttcct ggcgagcaac acaagcccgt
    20821 ccccaattac cgtagaatcc ctcaccgcgc tggattgttt caatataaga ttgatcagct
    20881 tgaaaatatt tctttttttc ataatatttt tcagccgctt tgaacgctgg ccgcgtctcg
    20941 ggtctgatat tttttttgat ctgccggtaa tgcaatccgt caggaagttt cgtaaaatcg
    21001 tctgacgccg aaaaggcaag ccctgcgaac aaaagtaaaa gtaagctgac aacatacttt
    21061 ctcacggcgg aaaatatgcc gaatttccga gcaaattcca aattaaaata tctgaacctt
    21121 tataatttca ccaccttgaa ctgcattaat ctttttaaat agccggttat ttatttaaac
    21181 ttttaaatta aataactcgg tccgctacga ttaaactcat taatgaattc accgaaccga
    21241 ttaccgattg attcatatcc agaataactt ttgaattaac ggtatatgtg attaatttcc
    21301 tattgttttc ggcgttggtt tgagcggcga gaagaccaag cgagtttttt tgataagcag
    21361 ttaaaaaacg aatgactatg cgccagatcc tctgttcacc aaaatttttc tctaatccct
    21421 ttcagaaatg cgataagttt ccagccagtt caggattaat ttacaattaa atgcaacaaa
    21481 ccaactgaaa tagtaatagt caatactttg gaatactttt aagaaaatat gaaatttaat
    21541 gcaggattaa gtctatcatg gctactaact ttatgg
//
LOCUS       DAOMJU010000030        21377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2306_length_21377_cov_16.879842, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000030 DAOMJU010000000
VERSION     DAOMJU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 21377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21377
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2306_length_21377_cov_16.879842"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..444
                     /locus_tag="PLO58_04475"
     tRNA            368..444
                     /locus_tag="PLO58_04475"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:402..404,aa:Val,seq:gac)
     gene            627..2570
                     /gene="thrS"
                     /locus_tag="PLO58_04480"
     CDS             627..2570
                     /gene="thrS"
                     /locus_tag="PLO58_04480"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQO74078.1"
                     /translation="MESNRKITIHLPDNSTMEVSAGVSAGQIAKEISPGLFQNALAAK
                     VNDRLVDLSTIISEDARLEILTYNSPESQEILLHSTSHVMAQAVKQLYPQAKVTIGPA
                     IENRFYYDFDIEPPLTDGDLPAIEARMNEIIAADLPIIRREVSREEAIELFSRMGETY
                     KVEIIREIEPDVPLSIYTQGDFTDLCRGPHIPSTGKIKAFKLLNVAGAYWRGDSRNKM
                     LTRIYGTSFATQKELKKYLNFIEEAKRRDHRKLGKSLELFEINEQVGPGLVLWYPRGA
                     LLLQNIKDYWIKEHLKRGYDLVQTPHIGKAQLWETSGHLDFYRESMFDGMTVEGETYY
                     IKPMNCPFHMQIYKSKTRSYRDLPIRYAELGTVYRYELSGVLHGLMRVRGFTQDDAHI
                     ICTPEQLNDEIQSLVLFSFEFIRSFGFKEMDVYVATRPKEKYVGSPENWAEATEALKA
                     ALSKLDIEYHIDEGGGAFYGPKIDIKIRDALNRSWQCTTIQFDFNEPERFNMDYIASD
                     GNKRRPYMIHRAIMGSLERFAGVLIEHTVGDFPLWIAPVQAVVIPVTNNQDQIATEFY
                     KLLIDNGIRAEINLQNDTIGAKIRDAELMKIPFMFIIGEREATGRQISIRRRKIGDTG
                     VMSWEEAIDLIVKEIQLKKENKS"
     gene            2567..3073
                     /gene="infC"
                     /locus_tag="PLO58_04485"
     CDS             2567..3073
                     /gene="infC"
                     /locus_tag="PLO58_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQO74079.1"
                     /translation="MEKEVRVNEQIRSQTVRLIDFDGKQIGIVSNKEALRISEDRGLD
                     LVEVSPNTNPPVCKIMDFGKYRYQQQMKERESKKKQHVVKIKEVRFRPHIGEHDFEMK
                     INAARRFLGEGYKVKITLMFRGREMAHQETGKELVQKIIEKLADVGTPEKNPVQEGRT
                     IVTYFTAK"
     gene            3085..3279
                     /gene="rpmI"
                     /locus_tag="PLO58_04490"
     CDS             3085..3279
                     /gene="rpmI"
                     /locus_tag="PLO58_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQO74080.1"
                     /translation="MPKMKTRRSAAKRFTLTGSGKVKRNKAYHSHILTKKSSKRKRNL
                     RQSEILKSADVKRVKKMLGV"
     gene            3290..3637
                     /gene="rplT"
                     /locus_tag="PLO58_04495"
     CDS             3290..3637
                     /gene="rplT"
                     /locus_tag="PLO58_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQO74081.1"
                     /translation="MPRANSSVPHHRKHRKIVGMAKGYRGARSKNYVSAKDAVEKALQ
                     YAYRDRRNKKRDFRSLWIARINAAANQHDISYNKFIHGLKKAGITLDRKALAEIAMHD
                     ADGFAILVALVKG"
     gene            3642..4658
                     /gene="pheS"
                     /locus_tag="PLO58_04500"
     CDS             3642..4658
                     /gene="pheS"
                     /locus_tag="PLO58_04500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQO74082.1"
                     /translation="MDILNRADEVRGQLQKDLQQVSADPQAAENLRIKYLGKKGLITT
                     LFADFNSIPAEQKRQVGKVLNILKQEAEAQIQALNERLKAQVAQDEYFDYSLPGLAPA
                     VGHLHPITQTLNQVISIFREVGFSVEFGPEVETDYYNFQALNFAENHPARDMQDTFFI
                     DEKRLLRTHTSPVQIRVMLKQKPPIRILAPGRVYRNEAINPRSHCVFHQVEGLYVDEN
                     VTFSELKTTLEMFAKRFFGSEVKTRFRPSFFPFTEPSAEMDVSCFICAGKGCRLCKNT
                     GWLEILGCGMVDPNVFVNVGIDPEKYTGYAFGMGIDRTALLKYGINDIRLYFEGDMRF
                     LEQF"
     gene            4663..7050
                     /gene="pheT"
                     /locus_tag="PLO58_04505"
     CDS             4663..7050
                     /gene="pheT"
                     /locus_tag="PLO58_04505"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQO74083.1"
                     /translation="MIVNTNWLKKYTDFPFSPAELAERLTAIGLEANLIEDKSTGLEN
                     IIVAEILAVRPHPNADRLTICTVSTGEKQLEVVCGAPNVKQGQKVPLALVETTLPNGM
                     TLKPTKIRGVISEGMLCAEDELGLSNDHSGIMILEQDAAVGSRLSDYLATDGKSLEID
                     LTPNRPDCASHIGVAREITLMTGNELRKPAINLKEIQQPTSDSIEIEIADPVGCPRYA
                     ARLIRGVKIGPSPDWLVKSLESIGQRSINNVVDAANYVLMETGHPLHTFDYAHLKGPK
                     IVVRRAKPGEKVVTLDGVERELSGDVLLICDSEKPVAIAGIMGLANSEINDDTRDVLL
                     ESAYFNPGVIRKGARFLGMQTEASYRFERGTDYEGLLYALNRLAALIVEVAGGQICRG
                     IVDNYPNPIKARRVVLRYERTNRLIGMNFEPDWIKKVLTGLGCEIINSAESEITVTVP
                     TWRPDLEREVDLIEEIVRVYGMSKVPSAKYIQIVPMETINERYDLIEKLRFFVAGCGY
                     SELYCNSLVSARAAEFGAESGDKLKLRNPLGQDMTYLRTSLLPGMIAAVKHNFNHYRT
                     DLRLFELGFVQAFAPQAETLAEESLHLGVILTGYLDEPNWAYPRRASNIFFLRGLVEA
                     IAKQFGLQPINFQKAANPYYKHLIQILTGEAKLGYLGELAPGYLDKEWDIQLPVNSME
                     LNVEVILKSLNLTKTYHEPPAFPFTERDISIIVDRTIPVDKVIENIRQNGGNLLREIR
                     FYDLYLGKNIDKKEKSLTFNLLFQANDRTLTDAEVDQIMATIYANLSRELNARLR"
     gene            7061..7261
                     /locus_tag="PLO58_04510"
     CDS             7061..7261
                     /locus_tag="PLO58_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74084.1"
                     /translation="MNIVNLNLLEEKIKSLLLLVTHLKEENEKLKSELKEGASSEVML
                     DLKKRQELRAKIEGMLELLKDY"
     gene            7311..7616
                     /locus_tag="PLO58_04515"
     CDS             7311..7616
                     /locus_tag="PLO58_04515"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQO74085.1"
                     /translation="MEEPNVNIVRVTIYGQEYSVKSRADRDYIISVANYVNEKMEEVE
                     STLRTIQPSIRVAILAAMNITDELFTANQEKDKIVSSVEEKAGFLIDIIDEQLKEEI"
     gene            7722..9269
                     /gene="rny"
                     /locus_tag="PLO58_04520"
     CDS             7722..9269
                     /gene="rny"
                     /locus_tag="PLO58_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQO74086.1"
                     /translation="MTWTYIIFAIGGAVVGTALSLYIYTQKLKRAQATATEIIANAQK
                     EAEEIKREARLEAKDEAIRIRNRAEEEARNKMKDVREAEKKLAKREFYLDRKTELCDK
                     KTEELNKREEELKRHSEIILHKEAELQNIIEAQNERLERIAGMSRKEAEEILMNNLLD
                     KAKESVNKKVKEIRENALAEANRDAKKIIVSAIQRSAADHTAETTVSVVNLPNDNMKG
                     RIIGREGRNIRHFESLTGIEIIIDDTPEAVVLSGFDPIRREVARIALEKLIADGRIHP
                     ARIEEMINKAEKEVDEEILRAAEEAIDEVGISQFPPELLKMLGKLKYRTSYGQNVLKH
                     SIEVAWLSGLMAAELGLNGELAKRAGLLHDIGKAIDRNVTGTHALIGADFVKKFRENP
                     IVINAIASHHEEVEATHPISAIVQAADQISGSRRGARGDTLESYIQRLEKLESIATSF
                     AGVTKTYAIQAGREVRVIVENGVVSDAEADNLAEEIAAKIQQELNYPGQVKVVVVREH
                     RSVSYAK"
     gene            9266..10147
                     /locus_tag="PLO58_04525"
     CDS             9266..10147
                     /locus_tag="PLO58_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HQO74087.1"
                     /translation="MSNPKLINGVEIAQKVKDDLRPRINRLKQKGITPGLAVILVGDN
                     PASQSYVNMKAKAFESMQLFSETIRLPGESSQEKILDLVNQLNHDSRFHGILVQLPVP
                     KHLDSNAIIQAISADKDADGIHPTNLGKMVLGIEAPLPCTPHGILMLLKYSDIDPAGK
                     HVVITGRSNIVGKPIANLLMQKRPLGNATVTVCHTHTRNLPEITRLADILIAALGQPE
                     FIDRSFVKPGVVVIDVGSNRIEDASSPKGYRWVGDVKFSEVAEIASAITPVPGGVGPM
                     TIAMLIANTVLLAEKTS"
     gene            10170..10625
                     /locus_tag="PLO58_04530"
     CDS             10170..10625
                     /locus_tag="PLO58_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQO74088.1"
                     /translation="MNIITTLRVSDLTHGLTDAELEALAGLFRCRHIKRDEIIFREGE
                     PSEELYIIAKGRVSVLIYSVSLPGKCEKIATLRDNEIFGEFAFIDGSPRSATVVAEED
                     TDLIFVDNVALFRFLDENEHIGYLIMRNIAKILTGKLRQTDLEMRNAIL"
     gene            10644..11246
                     /locus_tag="PLO58_04535"
     CDS             10644..11246
                     /locus_tag="PLO58_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQO74089.1"
                     /translation="MASASDFINIIKTRRTIRQFRQEQVDQAVIIEWVDCARLAPSAR
                     NLQPLEYFLITEPKLRERVFPLLRWASYIAPLGDPKEGQHPTGYLVVLINKDLMQSHY
                     AKDAGAAIENFILAAWANGVGTCWLASVEREKLRALLAIPEQYEIDSVVAFGYPAESP
                     VIENRNEIRYWLDEKNTLHVPKRPLASLLHLNSWNRHEVE"
     gene            11250..12005
                     /gene="lgt"
                     /locus_tag="PLO58_04540"
     CDS             11250..12005
                     /gene="lgt"
                     /locus_tag="PLO58_04540"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQO74090.1"
                     /translation="MYPELLHIGPVTIYTYGLMMAIAFIVCYLIFRAETKKLGDNPDF
                     ASNVVFWAAIGGIIGAKVFYLLENIPDLLADPKGMIFSGSGLVFHGGLIGGTIAVILV
                     LRRSQKSIGVYADIIGPLLLIGQGIGRIGCFFAGCCHGKACDLPWAVTFPNASPPANY
                     PVHPTQLYEAFLNFAFYFILVKVIRPKTTKVGQTMAIYLMFAGLERFLIEFIRVNPRV
                     WGNLTSAQFTSIGLFCAGLIILLFFAKTPKKSV"
     gene            12208..14925
                     /gene="polA"
                     /locus_tag="PLO58_04545"
     CDS             12208..14925
                     /gene="polA"
                     /locus_tag="PLO58_04545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQO74091.1"
                     /translation="MIKIFHIFMEKKETLFLIDGSALAYRSYFGTIRTHLATSRGKPT
                     GAIFAFVNSLKRILEEVKPDYLAIVFDAPEKTFRHQIYTDYKATREAMPEDLVAQLPV
                     MFEIVKAMNIPMLTVPGYEADDVIGTLAKAAKNKGLQVFVVTGDKDFMQLVDDDILVY
                     KPASGQKEVEILDKNAVVSKLGVQPDQITDYLGLVGDPSDNIPGIKGIGPAKAQPLLA
                     KFGSLEKVIEQAESIGNPRLTETIKNGAEIARLSKQLATIKTDVPLDINIESLKIKPV
                     NQAELERIYRELEFYSLIETKPEVSDAVKPDKHYQTIQTQAEVRELVKNLLNKELLSV
                     DLETTSINPMMAEIVGVALCWEAHSGVYLPIRHPAMADQLFAQTDNSAWLAELKPLLD
                     NEQIVKCGQNLKYDMLILRRNGIELRGKIFDTMVAAFLIQPDARSYKLDKLSQYYLNY
                     KMQSIEELIGNGKDQLNMEQVDIDKVTFYAVEDADVVLQLVPIFEEKLKADQLWQVFE
                     EIEMPLVPVLMQLELNGVYLDVKSLKEMSRNLEVQIDAIMRRIYDLAGVEFNINSPKQ
                     LGEILFEKLNLPKIRGNSTDVSVLEKLKHKHPLPSAVLEYRGLVKLKNTYLDTLPLLV
                     NQKTGRIHSSFNQTMAATGRLSSSDPNFQNIPIRTDIGREIRRAFIPQQAGWKILSAD
                     YSQIELRIMAHLSGDAELRRAFQEGVDVHARTAALVYGVPEKDVLPEMRRIAKVVNFG
                     IMYGAGPFRMSEELGISRSDANKLIEQYFRTYPGINDYITKTIAEARQNTYVKTLTGR
                     RRYVPDINSENKNVREAAERVAINMPIQGTAADMIKIAMIRIQRQLTKEKFKSMMILQ
                     IHDELLFEVHPDELERLRQIVTTEMENALPLDVPVKVDIGVGNSWYEAH"
     gene            14925..15848
                     /locus_tag="PLO58_04550"
     CDS             14925..15848
                     /locus_tag="PLO58_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQO74092.1"
                     /translation="MEASITLRNVSKFFKKKYVLSNLTLGIEKNTFFAIIGRNGAGKS
                     ILLRVISTFMAPDAGEVFINGEDIAKSPQMAKNCIGYLPDHDIHNRWITGWENLKARA
                     ELLEIPQSRFKEICEPLIKDFNLQEVLDEYPVTYSRGAKRCLDLVLVLLGDPQILILD
                     EPTLGLDYQNRASLFKYLLSVKNHKTIIVASNEFIEVQTLAERWIVLDYGKIRFDGTL
                     EKMLTRVDVPFSGEIILRKKDETLLEKIKESDNFSKVYDLDLTIQLTFERLQDFYAVL
                     ATIPAENLVGVNIQSLSIAEFINRLLSDEEF"
     gene            15851..16597
                     /locus_tag="PLO58_04555"
     CDS             15851..16597
                     /locus_tag="PLO58_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQO74093.1"
                     /translation="MIFKKIMLKNELRLDLHKILLGLFFNIIGTCLVYFALVTSLGKI
                     LPRIDYLEVDKFILSAIIGFTSGLMALGLASSYVTHYFQDSRGDYGRLPGMPISQIYG
                     SFLIRLWIILILHLLVTTLTVLILSGFFPGLGRLLLFWIFAILPLGIFIQLGMLIGLA
                     EDRRNQFYWIFLILVPLFLMSGMIIPTHFHTGFISKLLMALPSTVLIEGGRSILTRQP
                     FEFLNILYLLALNILLFIGGIFFFKRKLQN"
     gene            16594..17055
                     /locus_tag="PLO58_04560"
     CDS             16594..17055
                     /locus_tag="PLO58_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74094.1"
                     /translation="MRAYLAGAIEYAPDHGKVWRTEMEKFLQEEFGHSCYNPLIEEHK
                     YLTPDESRNFRGYKQTDPLRFQQIVRKLIQGDLRALETKIDYVICNWDKYAVKGGGTY
                     GEVTYAFYHNIPVYLVSEFPVIEISGWIIGCATKIFSSFNELKEYLKITYK"
     gene            17107..>18209
                     /gene="alr"
                     /locus_tag="PLO58_04565"
     CDS             17107..>18209
                     /gene="alr"
                     /locus_tag="PLO58_04565"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQO74095.1"
                     /translation="MHFPQNTTIAEVDLNNLRYNYQNIKQKVAPAQIMAVVKANAYGH
                     GAVPIAQCLRDQGVQYFAVARLCEAMELIQSGIGGEILIFGTLYDEEASFAIAHNIRI
                     TVTRAEDFVTYDRLAGALQKVAKVHVKIDTGMGRVGLPAIQAFDLIKASRKLKNIEIE
                     GICTHFATADSRDKTFAYEQLARFNQIIKQLRQMGITSPLIHAANSGAILDLPETYFD
                     MVRPGITLYGHYPSLETSESIPLRQVMSLKTRVAQIREVPGGTSISYGRLYFTKKVTR
                     IAVLNIGYADGMPRAFSNKGQVMIKGQLFPIIGAVTMDQLMVEVGDADVKPGDEVLIW
                     GNSPDGILSATQVAAKAGMLIYELCCAVAPRIPR"
     assembly_gap    18210..18309
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18310..18719)
                     /locus_tag="PLO58_04570"
     CDS             complement(<18310..18719)
                     /locus_tag="PLO58_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQO74096.1"
                     /translation="MKIHSIKSIIFDLDGTLVDTAADILAAVNSTMEIYGLKPITYEQ
                     CLRYLGDGVEDLVRRAVENSISEPIDPNYLAEIVAQYKDFYSSHLTDRSRLFPGVRET
                     LAELQDYTLVVVSNKSHDFCVQILRQLDIEKYFQL"
     gene            19156..>19433
                     /locus_tag="PLO58_04575"
     CDS             19156..>19433
                     /locus_tag="PLO58_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQO74097.1"
                     /translation="MKRTLIVIMTIAIFLLAFLGCSDEDNDSVTGANTDALPAYLPKG
                     TIHGLVRDMCTNVPIKGAVLSLSYNGEVTTVKSNSAGQFSFDNVPAGQ"
     assembly_gap    19434..19533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19534..21138
                     /locus_tag="PLO58_04580"
     CDS             <19534..21138
                     /locus_tag="PLO58_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74098.1"
                     /translation="ESAYVILQDLSITPGVIIKQTQTDANGYYRFNNVDNGINVSIMA
                     RSSDGKFEGSLPGGLSLLCNLTYDSLRSRVDAEKIQLAWVNDVAPYVINITPQNNSDV
                     SPSELAVIYTFSVPIKQTPYTRTDLGLGHKTILDDITVSFSGLKKTAGLLNTEISWNN
                     EMTQLIIQPKNVVGSAKYDIDVTSALPKLEDEAGLKVVDNPEIVGDFEKLYFTTAGQS
                     VVPTAPSLTRRIVPGAYEYLDYNGGTVNLEWNSDPSARSYNLYRQIGDGSFELIDSDI
                     FSLIVSDATGMLVTPAVNDPLSAITVKYQVCAVSKDLVEGPPSNTITISDDISPQLIG
                     TSVINLASQRYQFNFRFSEPLVISSAEDLNNYTIQDPDTVVFTKERAAYMGYNSTNND
                     YEVLLTVSISNGEVLPRGFSVILGNEIVDLAGRSINSSANTYVYSSPPTPVLFSPPDN
                     STNISSSQVLTWKISNGALSYRLQISTNVNFTSTIFDSDKITSTSFSLDAVSGLVSGA
                     KYYWRVQAANSVGSSAFSTTWSFTLQ"
BASE COUNT         6296 a         4228 c         4705 g         5948 t
ORIGIN      
        1 tgaaaatacc ttagaaaaaa atgaagaaag gagctaatag tcacatagca tgacatcgtt
       61 tacattcacc ccatggggtg aatcggtttt ttatgtaaag tattaagaca tggtatccac
      121 cttttagaaa taaataggat taacgcagag cagttttttg aatcaattaa taaaataaca
      181 aattcactac ctaaaatcgt ttagtatttt tattaaacag agaattatgt attaagcgga
      241 cctcaatgtc aatttttctt tttgcgtgat aaatatgctt tggttaaatt tgcattcgtt
      301 ttttgacgat aatgaattgg aataattatt atcaactttt ggaccattat tttaagttga
      361 tatatccggg cgattagctc aggtggctag agtacctgct tgacatgcag ggggtcactg
      421 gttcaagtcc agtatcgccc accaaagtga gtttttttac aatcgaattc aggttagatg
      481 gttaatatat ctatcctgat aaaaaccggg aacactggtt ctttattagg aaccccgatg
      541 ggtcagaaaa gtccagtatc gccctagtac aattgaaaaa ttaaatatag tcattatgac
      601 tctgaagcgc gaaaaggaag caaattttgg aatcaaatcg aaagattacg atacatttac
      661 ccgacaattc aactatggaa gtgtcggcag gtgtatcagc cgggcaaatc gctaaagaaa
      721 tcagtcccgg tctttttcag aacgctctcg ccgctaaagt caatgaccgc ttggttgatc
      781 tttcgactat tatttcggaa gatgctcgac ttgagattct gacctataat tctcccgagt
      841 cacaagaaat attactgcac agcacctcgc acgttatggc acaggcggtt aagcaattat
      901 atccacaggc aaaagtaacc atcggcccgg cgatcgaaaa tcgtttctac tatgattttg
      961 atatcgaacc acctttaacg gatggcgatt tgccggcaat cgaagcccgt atgaatgaaa
     1021 tcattgcggc agatttgcca attataagac gtgaggtttc ccgcgaggaa gcaattgaat
     1081 tattttccag gatgggtgag acatataaag ttgagatcat tcgtgagatt gaaccggatg
     1141 tgccattgtc aatttatact caaggcgatt ttaccgatct ttgccgtggt ccacacattc
     1201 cttcgaccgg caaaatcaaa gcctttaaat tattgaatgt tgctggcgca tattggcgcg
     1261 gtgattcgcg caataaaatg ctaacgcgta tttatggtac ttcttttgcc acacaaaagg
     1321 aacttaagaa gtacttgaat tttatcgagg aagccaagcg gcgcgatcat cgcaaactcg
     1381 gcaagagtct cgaacttttc gaaatcaacg aacaggttgg ccccggactg gtgctttggt
     1441 atccgcgtgg tgcgttgctt ttgcaaaata tcaaggatta ctggattaaa gaacatttaa
     1501 aacgcggcta cgatctagta caaacgcccc atatcgggaa agcccagctt tgggaaacga
     1561 gtgggcacct cgacttctat cgtgaatcta tgttcgatgg gatgacggtc gaaggcgaga
     1621 cttactatat caagccaatg aattgtccgt tccatatgca gatttataaa tcgaaaacgc
     1681 gtagttatcg cgacctgccg attcgttatg ccgaactggg gacggtttat cgctatgaat
     1741 tatctggcgt gttacacgga ttaatgcgcg tacggggctt tactcaagat gatgcccata
     1801 ttatttgcac gccggagcaa ctcaatgacg aaatccagag tttggtgctc ttctcatttg
     1861 aattcattcg ctcgtttggc tttaaagaaa tggatgtata tgtcgctacc cgcccgaaag
     1921 aaaaatatgt cggttcgcct gaaaattggg cggaagccac cgaggctttg aaagcggcac
     1981 taagtaaact cgatattgaa tatcacatag acgaaggtgg cggcgctttt tacggtccca
     2041 aaattgacat taaaatccgt gacgctttga atcgttcctg gcaatgtacg actattcaat
     2101 tcgattttaa cgagccggaa cgtttcaata tggattatat cgccagtgac ggcaataagc
     2161 gtcggcctta tatgattcac cgcgcgatta tgggttcgct ggaaagattt gctggcgttt
     2221 tgattgaaca taccgtcgga gattttccgc tatggatcgc gccggttcag gcagttgtca
     2281 ttccggtgac aaataatcag gatcaaatcg caacagaatt ttataaatta ttaattgaca
     2341 atggaattcg agctgaaatc aatttgcaaa atgataccat tggtgctaaa atacgcgatg
     2401 ctgagctgat gaaaatacca tttatgttta taattggaga aagagaagcc actggacgac
     2461 agatttcgat tagacgtcga aaaatcggcg ataccggcgt aatgagctgg gaagaagcca
     2521 ttgatttaat tgttaaagaa atccaattaa agaaggagaa caagtcatag agaaagaagt
     2581 acgggtaaac gaacaaattc gaagccaaac tgtccgtcta atagactttg atgggaaaca
     2641 gattggcatt gtcagtaata aagaagctct acgaatatcc gaagatcgtg ggcttgattt
     2701 ggtggaagta tcaccgaaca ctaatccacc cgtctgtaag ataatggatt ttggtaaata
     2761 tcgttatcaa caacaaatga aagagcgtga gagtaagaaa aaacagcatg ttgttaagat
     2821 taaggaagtt cgtttccgac cgcatatcgg ggaacatgat tttgaaatga aaatcaacgc
     2881 cgcccgacga ttcctcggcg aaggttacaa agtcaagatc actctaatgt tccgcggtcg
     2941 cgagatggct catcaggaaa ccggaaaaga actggtgcaa aaaattatcg agaaactagc
     3001 tgacgtcgga acacctgaaa aaaatcctgt ccaagaaggg cgaaccatcg ttacttactt
     3061 cacggctaaa taggagaaaa aaatatgcct aaaatgaaaa ccagaagatc agcggctaaa
     3121 cgttttacct tgaccggaag tggaaaagtt aaacgtaaca aagcctatca ttctcatatt
     3181 ttgactaaaa aatccagcaa acgcaaacgc aatttaagac aatctgaaat tcttaaaagc
     3241 gctgacgtta aacgcgttaa aaagatgctt ggagtatagg agtaaaatta tgccaagagc
     3301 aaatagttcc gttccccatc atagaaaaca tcgtaaaatt gtcgggatgg ctaaaggcta
     3361 ccgtggcgca cgcagtaaaa attacgttag tgccaaagat gccgtcgaaa aagccttaca
     3421 atatgcctat cgcgatcgtc gcaataaaaa acgcgatttt cgctctctct ggattgcgcg
     3481 cattaatgcc gccgctaatc aacatgacat ttcctataat aaatttatcc acggtctgaa
     3541 gaaggctggg attactctcg accgtaaagc tttggccgaa attgccatgc acgacgccga
     3601 tggatttgcg atattggtag cgctggtaaa aggctgagag tatggacatc ctgaatcggg
     3661 ctgatgaggt tcgggggcaa ctgcaaaaag atcttcagca ggtcagcgcc gatcctcaag
     3721 cggctgaaaa tctcagaatt aaatatttag ggaaaaaagg tctaatcacg acgctttttg
     3781 ccgatttcaa ttccattccc gccgagcaaa aacgccaggt tggtaaagtg ctcaatattt
     3841 tgaagcaaga ggctgaagct cagattcagg ctttaaatga acgactgaag gcgcaggtcg
     3901 ctcaggatga atatttcgat tacagtttac caggtctggc gcctgccgtt ggccatttac
     3961 acccgattac ccaaacactc aaccaggtca tttctatttt tcgagaagtc ggttttagtg
     4021 tcgaatttgg gccggaagtc gaaaccgatt attataattt ccaagcttta aactttgcgg
     4081 aaaatcatcc agcgcgcgat atgcaagaca ccttcttcat tgacgagaaa agactgctga
     4141 gaacccatac ttcaccggtt caaattcgcg taatgttaaa gcaaaaacca ccgatccgga
     4201 ttttagcccc gggaagagta tatcggaatg aagccatcaa tccgcgcagt cattgcgttt
     4261 ttcaccaagt tgaaggacta tacgttgatg aaaatgtaac cttcagcgaa ttgaaaacaa
     4321 cactcgaaat gtttgcgaaa cgctttttcg gtagcgaggt aaaaactcgt tttcgtccca
     4381 gttttttccc gttcactgaa ccaagtgctg aaatggatgt ttcctgtttt atttgtgctg
     4441 gtaaaggttg ccgactctgt aaaaatactg gctggttgga aattctcggt tgcggtatgg
     4501 tagatccgaa cgtttttgtc aatgtcggca ttgacccgga gaaatatacc ggttatgctt
     4561 tcggcatggg aatcgaccga acggcgttgt taaaatacgg cataaatgac attcgccttt
     4621 attttgaagg tgacatgcgc tttctggaac agttttaacc ttatgatagt caatacaaac
     4681 tggctaaaaa aatacaccga ttttcccttc agcccggcag aattagctga acgtctgact
     4741 gccatcggct tggaagccaa tttaatcgag gataaaagca ccggtttaga aaatatcatc
     4801 gtcgctgaaa tattagctgt tcgaccgcat cctaatgccg accgtctgac gatttgcacc
     4861 gtttcaaccg gagaaaaaca actcgaggta gtctgtggcg cgcctaatgt caaacaaggg
     4921 caaaaagtcc cgctggcact ggtggaaacc acattaccga atggtatgac gcttaaacca
     4981 actaaaatca gaggtgtaat ctccgaaggt atgctttgtg cggaagatga actggggctt
     5041 tccaacgatc atagtgggat tatgatcctg gaacaagatg cggcggttgg tagtcgcctt
     5101 tctgattatt tggcgactga tggaaagagt ctggaaattg acctgactcc caatcgccct
     5161 gactgcgcat cgcatattgg agtcgctcgt gaaatcacat tgatgaccgg caatgaacta
     5221 cgcaagccag ccataaatct caaagaaatc cagcaaccaa ctagcgattc catcgaaatc
     5281 gaaatcgccg atccggttgg ttgcccgcgc tatgccgccc gcctaattcg cggtgtaaaa
     5341 atcggtccct caccggattg gttagttaaa agtttagaaa gtattggaca gcgttccatc
     5401 aataatgtgg tagatgccgc caattatgtc ttaatggaaa ccggacaccc gctccatact
     5461 ttcgattatg cccatctcaa agggccgaaa attgttgtac gtcgggctaa accgggcgaa
     5521 aaagtcgtta cgcttgatgg agttgaacgc gaactttcgg gcgatgttct cttaatttgc
     5581 gatagcgaaa agccggtagc aattgccggg attatgggtt tggcgaatag cgagatcaat
     5641 gatgatactc gcgatgtctt gctggaaagt gcttatttca atccgggcgt gattcgaaaa
     5701 ggagcgcgtt ttctgggtat gcaaaccgaa gcgtcttatc gctttgaacg cggcaccgat
     5761 tatgaaggtc tcctctatgc gctgaaccga ctggcggccc taattgtaga agtagcgggc
     5821 ggtcagattt gccgaggtat cgtggataat tatcccaatc cgattaaagc tcgcagagtt
     5881 gttttgcgtt atgaacgtac taatcgtttg atagggatga attttgaacc ggattggatc
     5941 aaaaaagtac tgactggatt aggttgcgaa attattaata gcgctgagag tgaaatcacg
     6001 gttactgtcc cgacctggcg gcctgatctc gagcgagaag ttgacctgat cgaggaaatt
     6061 gtccgcgttt atggaatgag caaagtaccg tcggctaaat atatccagat tgttccaatg
     6121 gaaactatca acgagcgcta cgatttgatt gaaaagttgc ggtttttcgt agccggttgt
     6181 ggctattccg aattgtattg caattctttg gttagtgcca gagcggctga atttggagct
     6241 gaatctggtg ataagttaaa actgcgtaat ccactcggtc aggatatgac ttatctgcga
     6301 acctcattat tacctggtat gattgcggcg gttaaacata atttcaatca ctatcgcacc
     6361 gatctaagac ttttcgaact gggttttgtg caagctttcg caccgcaagc cgagactctg
     6421 gctgaggaat cacttcatct gggagtaatt ttaacgggat atttagatga gccgaattgg
     6481 gcatatccac gccgcgccag taatattttc tttttaagag gactggtaga agccattgcc
     6541 aaacaatttg gtcttcagcc tatcaatttt caaaaagccg caaatcctta ttacaagcat
     6601 ttaattcaaa tcctgactgg tgaggctaaa ttgggttatt tgggggaatt agcccccggt
     6661 taccttgata aggaatggga cattcagtta ccggtaaaca gtatggaatt aaatgtggaa
     6721 gtcatcctga aaagtttgaa tttgactaaa acgtatcacg agccgccagc ttttccgttt
     6781 accgaacgcg atatttcaat catagtagat cgcacgattc ccgtggataa agtcattgaa
     6841 aatattagac aaaacggtgg aaatttactc cgtgaaatca ggttctatga tctatacctt
     6901 ggaaaaaata ttgacaaaaa ggaaaaaagt cttacattta acctgttatt tcaagctaat
     6961 gatcgaacct taaccgatgc cgaagttgat caaattatgg caactattta tgccaattta
     7021 tcacgggaat tgaatgcccg tttaagatga gaagagcaaa atgaacatcg tcaatttgaa
     7081 tttattagaa gaaaaaatca aaagtttgtt gttgctcgta actcatctta aagaagaaaa
     7141 tgagaaattg aagtcggaat taaaagaagg agcctcttcg gaagtaatgt tagaccttaa
     7201 aaaaagacag gaacttagag ctaaaatcga aggtatgctc gaacttttaa aggattatta
     7261 atccctgtcc aaccgtaacg ttatttgaaa caaaaattga tttaaaaaag atggaagaac
     7321 ctaacgtaaa tatagtccgt gtaactatct atggtcagga atacagtgta aagtccagag
     7381 ccgatcgcga ctatattatc agcgttgcca actatgtcaa tgagaagatg gaggaagtcg
     7441 aatctacgct ccgaactatt caaccctcta tccgcgtagc tattttagcc gcaatgaata
     7501 ttaccgatga attatttaca gccaatcaag aaaaagataa aatagtcagt tccgtcgagg
     7561 agaaggcagg tttcttgatt gatatcattg acgagcaact taaagaagaa atttaatcca
     7621 tcttaaataa ataaatcaga ttaactctac catccttatt acggttggaa ttatctaaaa
     7681 ttaacggtag agttctaacc gattctataa ggagaagtgt aatgacctgg acctatatta
     7741 tcttcgcaat aggcggagca gttgttggga ctgccttatc gctttacata tatacccaaa
     7801 aattgaaaag ggcacaggcg accgcgacgg aaattatcgc caacgctcaa aaagaagccg
     7861 aagaaatcaa acgagaagct cgtctcgaag ccaaagacga agccattcgt atccgaaacc
     7921 gcgccgaaga ggaagcccgc aacaaaatga aggatgtgcg cgaagccgaa aaaaaactcg
     7981 ccaagcgcga gttctatctc gaccggaaaa ccgaattatg tgataaaaaa accgaagagc
     8041 tcaataaacg ggaggaagaa ttaaaaagac atagcgaaat tatcctccac aaagaagccg
     8101 aacttcagaa tattattgaa gctcagaatg aacgactgga gcgcattgcc ggtatgtccc
     8161 gcaaggaagc cgaagaaatt ctaatgaaca atctgcttga taaagctaaa gaaagtgtca
     8221 ataaaaaggt caaggaaatc cgcgaaaatg ccttagccga agccaatcgc gatgctaaaa
     8281 aaattatcgt ctcggctatc cagcgctcgg cggctgacca tacggccgaa acgactgtct
     8341 cagtggtcaa tttgccgaat gataatatga agggacgtat tattggacgc gagggacgca
     8401 atatccgcca tttcgagtca ttgaccggaa ttgaaattat tattgacgat acgccggaag
     8461 ccgttgtcct ttccggtttt gatccaatac gtcgcgaagt tgcccgcatt gctctggaaa
     8521 aattaattgc cgatgggcga attcacccgg cgcgcatcga agaaatgatt aataaagcgg
     8581 agaaggaggt tgatgaggaa attttgcgtg cggcggaaga ggctattgat gaagttggca
     8641 tttctcaatt tccgccggaa ttgctaaaaa tgctcggcaa actgaaatac cgcacctcct
     8701 atggtcaaaa tgttttaaaa cattccattg aagtcgcctg gttatccggc ttaatggcgg
     8761 ctgaattagg attaaatggt gaattagcca aaagagccgg tctattgcat gatatcggca
     8821 aagctattga tcgaaatgtc actggtaccc acgccttgat cggtgcggat tttgtcaaaa
     8881 aattcagaga aaatccgata gtaattaatg caattgcctc tcatcatgag gaagtcgaag
     8941 cgactcatcc gatttctgcc attgtacaag ccgctgacca gattagcggt tcacggcgcg
     9001 gagcacgtgg tgatacgctg gaaagttata ttcagcgttt ggaaaagctg gaatccatcg
     9061 cgacttcctt tgccggggtt acgaagactt acgctattca agccggacgc gaagtacgtg
     9121 tcatcgtcga aaatggggtg gttagcgatg cagaagccga taatctggca gaagaaatcg
     9181 ccgccaaaat ccagcaggaa ctcaattacc ccggtcaggt aaaagttgtc gttgttcgtg
     9241 aacatcgtag tgtgagttat gccaaatgag taatccaaaa ttgattaacg gcgttgaaat
     9301 cgcccaaaaa gttaaagacg atctcagacc gcgtataaat cgtcttaaac agaaaggaat
     9361 cacgccgggc ttagcggtca tattagtcgg cgataatccg gcttcccagt cttatgtcaa
     9421 tatgaaagcc aaagccttcg agagtatgca acttttttct gaaactattc gtctgccggg
     9481 cgaaagttcg caggagaaaa ttctggactt ggtaaatcaa ttaaaccacg attcgcgatt
     9541 ccacgggatt ctggtgcaat taccggtgcc aaagcatctg gacagtaatg ccattattca
     9601 ggctatttca gccgataaag acgcggatgg cattcatccc accaatttgg ggaaaatggt
     9661 tttggggatt gaagcgccat taccctgcac gccgcatgga attttaatgc ttttgaaata
     9721 ttccgatata gacccagccg gtaaacatgt agttataact ggtcggagta acatcgttgg
     9781 caagccgatt gctaatttat tgatgcagaa acgaccgctc ggtaatgcca cagttacggt
     9841 ttgtcatacg cacactagga atctgccaga aattacgcgt ctggctgata ttttgattgc
     9901 cgcgctgggc cagccggagt ttattgatcg ttcatttgta aaacccggag tggtggtgat
     9961 tgatgtcgga tccaatcgga ttgaagatgc atcatcacca aaaggctatc gctgggtcgg
    10021 cgatgtcaaa ttcagtgaag tagccgaaat tgcctccgct atcacgccgg taccgggcgg
    10081 agtcggtccg atgacgattg ctatgctgat tgccaacact gttttattgg cagaaaagac
    10141 atcataaaat taataacaca ggagggatga tgaatataat taccactctg cgagtttcgg
    10201 atttgacaca tgggttgaca gatgctgaac ttgaggcttt agccggtttg tttcgctgtc
    10261 gtcacatcaa acgagacgaa ataattttca gggaaggtga acccagtgaa gaattatata
    10321 ttatagcaaa agggcgggtg agtgttttga tttattcggt atcacttccc ggcaaatgcg
    10381 aaaaaattgc caccctgcgt gataatgaaa ttttcggaga atttgcgttt attgacggct
    10441 caccacgctc agcaacagtt gtggctgaag aagataccga tctcattttt gtagataatg
    10501 tcgctttatt tcggttttta gatgaaaatg agcatatagg ctacttaatt atgcgcaaca
    10561 tcgctaaaat tcttaccggc aaattacgtc aaaccgatct cgaaatgcgg aacgctattc
    10621 tttaaatccg aaagggtctg tttatggctt cagcttctga ttttatcaat attattaaaa
    10681 ctcgccgtac aatccgccag tttcgtcagg aacaagttga tcaagccgtt attatcgaat
    10741 gggtggattg cgcccgctta gcaccgtccg ctcgcaatct ccaaccactt gagtattttc
    10801 tgattaccga gccgaaatta agggagcgag tttttccact tttacgctgg gcgagttaca
    10861 ttgcaccatt gggtgatccc aaagagggac aacatccgac cggttatttg gtggttttga
    10921 taaataaaga tttaatgcaa tcgcattatg ctaaagatgc cggcgcggcg atagaaaatt
    10981 tcattctggc ggcttgggca aatggagttg gcacttgctg gcttgcttca gtcgaacgcg
    11041 aaaaattgcg ggctttgctc gcaattcccg agcaatatga aattgactcg gtggttgctt
    11101 ttggctatcc ggctgaatcg cctgtgatag aaaataggaa tgaaattcgc tattggctgg
    11161 atgagaaaaa tactcttcat gtccccaaga gaccattagc cagtttatta cacctaaaca
    11221 gttggaatag acacgaggtg gaatgaaata tgtatcctga acttctgcat atcggtccgg
    11281 tgactattta tacttatggt ctaatgatgg cgatcgcttt tattgtttgc tatttgatat
    11341 tccgagccga gactaaaaaa ctcggcgata atcctgattt cgccagtaat gttgtatttt
    11401 gggcggcaat cggtggaata atcggggcaa aagtatttta tctactggaa aatattccgg
    11461 atttattagc tgatccgaaa gggatgatct tttccggttc cgggcttgta tttcacggtg
    11521 gattgatcgg tgggacaatt gccgtgattc tggttttacg ccgctcgcaa aaaagtattg
    11581 gcgtttacgc tgacatcatt ggtccattgc ttttaatcgg gcagggtata gggcgtatcg
    11641 gctgtttttt tgccggttgt tgccatggta aagcctgtga tttaccctgg gcagtaactt
    11701 ttcctaatgc ttcaccgccg gctaattatc cggtgcatcc gactcagctt tatgaggctt
    11761 tcttgaactt tgctttttat tttatcttgg taaaagtaat tcgccccaaa acaaccaaag
    11821 tcgggcaaac tatggcaatc tacttgatgt tcgccggact ggaacgcttc ctgattgaat
    11881 ttatccgagt caatccgcgc gtttggggga atctcaccag cgcccagttt acttctattg
    11941 gattattctg tgccggatta ataattctcc tattctttgc caaaactcct aagaaaagtg
    12001 tttaaaagta cttttaagtc cgcttaaatt ctcttaagtg cttttaaaat attgagatat
    12061 ttccgtaata ttccgtttta aatatacctt caagccttaa aactattttt ttgacaacaa
    12121 ttttcttaat aacactaaaa acacttatca gcacttaaca acacttgctc tacttttcac
    12181 tgaaatcgtt tggcaaattg ccagttaatg attaaaattt tccatatttt tatggagaag
    12241 aaggaaacat tatttttaat agatgggtcg gctttagcgt accgttctta cttcgggacc
    12301 attcgcaccc atttggcgac ttcccgaggg aaaccgactg gcgcgatatt cgctttcgtt
    12361 aattcattga aacgcatcct cgaggaagtc aaacccgatt atctggctat agttttcgat
    12421 gccccggaga agacgttccg ccatcagatt tataccgatt ataaagctac tcgcgaagcg
    12481 atgccggaag acttagtggc tcaattgccg gttatgtttg aaatcgttaa ggcgatgaat
    12541 attccgatgc tcacagtacc cggttatgaa gctgatgacg tgattggtac gcttgccaaa
    12601 gccgccaaaa acaaaggctt gcaggttttc gtggttactg gtgataaaga ttttatgcaa
    12661 ttggtggatg atgatatttt ggtctataaa ccggcttccg gacagaaaga ggttgagatt
    12721 ctcgataaaa atgcggtagt cagcaaatta ggtgttcagc ccgaccaaat tactgattac
    12781 ctcggtctgg tcggcgatcc ttccgacaat attccaggga ttaagggcat tggtcctgct
    12841 aaggcacaac cacttttggc taaatttggc tcccttgaaa aagtgatcga acaggctgaa
    12901 tcaatcggca accctcgcct aacagaaacg ataaaaaatg gggcggagat tgcccggctt
    12961 tctaaacaat tagccacgat taaaactgat gttccgctgg atattaatat tgagtcgtta
    13021 aaaattaaac cggtcaacca ggcggaactg gagcgtattt atcgcgaact tgaattctac
    13081 tcgttaatcg aaacgaaacc cgaagtctca gatgccgtca aaccggataa acattaccaa
    13141 accatccaga ctcaggctga agtgcgcgag ttggtaaaaa acttacttaa caaagaatta
    13201 ttatcggttg atctggaaac gacttctatt aatccaatga tggctgaaat cgtcggagtt
    13261 gcgctgtgct gggaagccca ctccggggtt tatttgccga tcagacatcc ggcaatggct
    13321 gaccagcttt tcgctcaaac cgataattca gcatggttag cggagcttaa gccgctttta
    13381 gataatgagc aaatcgttaa atgtgggcag aatctgaaat acgatatgct gattttgcgc
    13441 cgcaatggta ttgaattgcg cggcaagatt ttcgatacta tggtagccgc tttcctgatt
    13501 caacccgatg ctcgctctta caaactcgat aaattgagtc agtattattt gaattacaaa
    13561 atgcagtcaa tcgaagaatt gatcggaaac ggcaaagatc aattgaatat ggagcaggtt
    13621 gatattgata aagtgacatt ctatgccgtc gaagacgccg atgttgtttt acaactggta
    13681 ccgatattcg aagagaagct gaaggctgat caactctggc aggttttcga agagatcgaa
    13741 atgcccctcg ttccggtttt aatgcaactg gaattaaacg gcgtttatct ggatgtgaaa
    13801 agtttgaagg aaatgtcacg caatctggaa gtccaaattg atgccattat gcgacgaatt
    13861 tatgatctgg caggtgtcga gttcaatatc aattctccca aacaactcgg cgaaatccta
    13921 ttcgaaaaac tgaacctacc caaaatccgc ggaaattcga ccgatgttag cgttctggag
    13981 aaattaaagc ataaacatcc tctgccgtct gccgtgcttg aatatcgcgg tttagtgaaa
    14041 ttaaagaaca cttatttaga tactttgcca cttctggtta accaaaaaac tgggcggatc
    14101 catagttcat tcaatcaaac tatggccgca accggacggt tgagtagcag tgacccgaat
    14161 ttccagaata ttccgattcg caccgacatt ggtcgcgaga ttcgtcgggc attcatacct
    14221 cagcaagctg gctggaagat tttatcggct gattattctc aaattgaatt gcggataatg
    14281 gcgcatttat ccggtgatgc cgaacttaga cgcgcatttc aggaaggggt agatgtgcat
    14341 gcccgcacgg cagccctggt ttatggcgtt cccgaaaaag atgtcttgcc ggaaatgcgc
    14401 aggatagcca aagtcgttaa cttcggcatt atgtacggcg ccggtccatt tcgaatgtca
    14461 gaagagctcg ggatttcacg ttccgatgcg aataagctca ttgaacaata tttccgcact
    14521 tatcccggca ttaatgatta tataacaaaa acaatcgccg aagcccggca aaatacttac
    14581 gtaaaaacct taaccggtcg gcgacgctat gttcccgata ttaatagtga aaataaaaat
    14641 gtgcgggaag ccgctgaacg cgtggcaatc aatatgccga ttcaaggcac agccgccgat
    14701 atgatcaaaa tcgcgatgat tagaattcag cgtcaattaa ctaaagaaaa attcaaatca
    14761 atgatgatac tccagattca cgatgaactt ttatttgaag ttcatccgga tgaattggaa
    14821 cgtcttcgtc aaatagtcac taccgaaatg gaaaacgctt tacctctgga tgttccggtt
    14881 aaggtagata ttggagttgg taattcatgg tatgaggcgc attaatggaa gcttctatta
    14941 cgcttagaaa tgtaagcaaa tttttcaaga agaaatatgt tctgagtaat ctgactctcg
    15001 gcatcgaaaa aaatacattc ttcgctataa ttggcaggaa tggagctgga aagtctattc
    15061 ttttgagagt aatatcaact tttatggcac cggatgcggg agaagttttt atcaacggcg
    15121 aagatattgc caaatccccg caaatggcta aaaattgtat aggttactta cccgatcacg
    15181 atattcataa ccgctggatt accggctggg aaaatctcaa agccagggct gaactactgg
    15241 aaatacctca gtctcgtttc aaagagattt gtgagcctct tatcaaagac ttcaatttac
    15301 aagaagtgct ggatgaatat ccggtgactt attcacgcgg cgctaagcgc tgtcttgacc
    15361 ttgttctggt tttgctcgga gacccgcaga tattaatttt agacgaacca actttaggtc
    15421 tggattacca gaaccgcgca tcgttattta aatacttact ctccgttaaa aaccacaaga
    15481 cgattatcgt cgcgtcaaat gaatttatag aagttcaaac tcttgctgaa cgctggattg
    15541 ttctggatta tgggaaaatt agattcgacg gcaccctcga aaaaatgtta acccgagtag
    15601 atgtgccatt ttccggggaa attatcttgc gcaaaaaaga tgagacttta ctggaaaaaa
    15661 ttaaggaatc ggataatttt tctaaagtct atgatctgga tttaaccatc cagctgactt
    15721 ttgaaagact ccaagatttc tatgccgtac tggctacgat tcccgctgaa aatctggtcg
    15781 gagtgaatat ccagtctcta agtatcgctg aatttattaa ccgtttatta tctgatgaag
    15841 agttttagta atgatattta agaaaataat gttaaagaat gagttacgcc ttgatttgca
    15901 taaaattttg ctgggcttat ttttcaatat tatcggtact tgtttagtgt attttgctct
    15961 ggtaaccagt ttggggaaaa ttctgccgcg aattgattac ctcgaggttg ataaatttat
    16021 attgtctgcg atcataggat ttaccagcgg actgatggcg ttaggattag ccagttccta
    16081 tgtgacgcac tattttcagg acagccgcgg tgactatggg cgccttcccg gaatgccaat
    16141 ctcacaaatt tatggcagtt tcctgattcg actgtggata attctaattc ttcatctgct
    16201 tgttacgacc ttaaccgttc tgatactttc ggggtttttc cccggattag gcagactatt
    16261 acttttctgg atatttgcaa tattaccgct tggtatattt atccagttag gtatgttgat
    16321 tggtttagca gaagatcggc gtaatcagtt ttactggatt tttctaatac tcgtgcccct
    16381 atttttaatg tccgggatga tcattccaac ccattttcat acaggattta taagtaaatt
    16441 gctgatggct ttgccatcca cagtattaat cgaaggcggg cgatccattt taacgcgtca
    16501 gccatttgag ttcctaaaca ttttatattt gctggcgctc aatatcttgc tttttattgg
    16561 tgggattttc tttttcaaaa ggaaattgca aaattgagag cctatttagc cggtgcgatt
    16621 gaatacgccc ccgaccacgg gaaggtctgg cggacagaaa tggaaaagtt tttacaggaa
    16681 gagttcggac actcctgtta taatcctttg attgaggaac ataaatattt gacacctgac
    16741 gaatcgcgta atttccgcgg atataaacaa actgaccctt tgcgttttca gcaaattgtc
    16801 cggaaattga ttcaaggtga tttacgggca ttggaaacca aaatagatta tgttatttgc
    16861 aattgggata aatacgccgt gaagggtgga ggcacctatg gcgaggtaac ctatgctttt
    16921 taccataata ttccggttta tttagtttca gagtttccgg taatcgaaat tagtggctgg
    16981 attatcggct gtgctactaa aattttctct tcgttcaatg aattaaaaga atatttaaaa
    17041 ataacatata aataaaaatt aaaaagatga aagccatctc gataaaaaaa tatgattgga
    17101 tagaatatgc attttccgca aaacacaacc atcgcagaag tagatctcaa taatcttcgc
    17161 tataactatc aaaatataaa acaaaaagtt gcacctgctc aaattatggc cgttgtcaaa
    17221 gccaatgcct atggccacgg cgcggtccct atcgctcaat gtctgcgaga tcaaggtgtc
    17281 caatactttg cggtagcgcg tctgtgcgaa gcaatggaac taatccaatc gggcatcggc
    17341 ggggaaattt tgattttcgg aacactctac gatgaagaag catcctttgc gatcgcgcat
    17401 aatatccgga taaccgtaac ccgtgcggaa gatttcgtaa cctatgaccg cttggcgggc
    17461 gctttgcaga aagttgcgaa agtgcacgtc aaaattgata ctgggatggg acgagtcgga
    17521 ttaccggcta ttcaggcttt tgatcttatc aaagcctctc gaaaattgaa aaacattgaa
    17581 atagaaggta tttgtaccca ttttgcaact gctgatagcc gtgacaaaac ctttgcgtat
    17641 gaacaattag cccgctttaa tcaaattata aagcaactac gtcaaatggg gataacttcc
    17701 ccactcattc atgccgccaa tagcggcgcc attctggatc tgccggaaac ctattttgat
    17761 atggtgcggc ctggaattac gctttatggt cattatcctt cgttagaaac tagcgaatct
    17821 atcccactcc gccaggtaat gtctttgaag actcgggttg cccagattag agaagtgcca
    17881 gggggaacca gcattagcta cggacgtctt tattttacaa agaaagtaac tcgtattgcc
    17941 gtcctgaata tcggctatgc cgatgggatg ccccgagcct tttccaataa aggacaggta
    18001 atgatcaaag gtcagctttt tccgataata ggagccgtca caatggatca attgatggta
    18061 gaagtcggcg acgccgatgt caagcctggt gatgaagtat tgatctgggg caattcaccc
    18121 gatggcatat tatccgctac tcaagtagcc gcgaaggctg ggatgctgat ctatgaacta
    18181 tgttgtgccg ttgctccgcg tatcccgcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18301 nnnnnnnnna gctggaagta tttctcgatg tccaattgtc ttaagatttg gacacaaaag
    18361 tcatggctct tattggaaac tactactaat gtataatcct gtaattcagc cagagtctcg
    18421 cgcacaccgg gaaatagacg agagcgatcg gttaaatgag atgaataaaa atccttatat
    18481 tgggcgacaa tctcagccag atagtttgga tcaatcggtt cactaattga attttcgact
    18541 gctcgtctga ctaggtcttc gactccatcg cccaaatacc tcaggcactg ctcataagtg
    18601 atgggtttca agccataaat ttccattgtg gagtttaccg cggctaaaat atcagcagca
    18661 gtatcaacca gagtaccatc aagatcaaag ataatagatt taatcgaatg aattttcata
    18721 gtggcaaata taattagata gcaggcattt cgacaaacct tccgtagtat caaaattggt
    18781 ctcaatttta gaaagttaaa cctctccgag gtggggaaat attttgagcg aataattact
    18841 acaaaacttc aagccctata gactagtcgg aaataaatga taggtatgtc aacgctaacc
    18901 gcgtagtgag caaggtattg tagagaaata gaatcacaaa tgtttcaata tgattttgat
    18961 aaagctcctt atttgaaatt ttgcttttag tttttgaaat aaatccttac tttaaaaaga
    19021 atttatgcgc atttattttt accatactaa gacatttgga tggagtaaat tttaatcaat
    19081 atagcaaatg aaccaggaca tttcacataa agggataatt caatataaaa ttattaacca
    19141 attgtaggag gatgtatgaa acgaacatta attgttatta tgacaattgc gatttttctg
    19201 ttggcttttc ttggctgtag cgatgaagat aacgattctg taacaggagc caatactgac
    19261 gctcttcccg cgtacctacc aaaaggtact atccacggtt tggtcagaga tatgtgtacg
    19321 aatgtgccga taaaaggggc agttctttca ttatcgtata atggtgaggt aacaacggtt
    19381 aaatccaata gcgccgggca gttttccttt gataatgtcc ctgctgggca attnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaatcag cgtacgttat tttacaggat
    19561 ctttcaatta caccaggggt aataataaag caaacccaaa ccgatgcaaa cggctactat
    19621 cggtttaata atgttgataa tgggataaat gtttctatta tggcgcgtag ttcggatggg
    19681 aagtttgaag gtagtttgcc aggtggatta tcgctactat gtaacctgac ttatgattct
    19741 ttgcgctcac gcgtggatgc cgagaaaatt caactcgctt gggtaaatga tgtagcccct
    19801 tatgttatta atattacacc tcaaaataat tccgatgttt ctccttccga gttagcggtt
    19861 atttacacct tctcagttcc aattaaacaa acgccctata cacgaacgga tcttggtctc
    19921 ggtcataaaa caattcttga tgatattaca gtttcgtttt ccggactcaa gaagacagcc
    19981 gggctgttaa atactgaaat ttcttggaat aatgagatga cccagcttat catccaaccg
    20041 aaaaacgttg ttggttcggc taaatacgat attgatgtta cttctgcttt accaaagttg
    20101 gaagatgagg ctggtttaaa ggtcgtagat aatccagaaa tcgtgggcga ttttgagaag
    20161 ttgtatttta cgactgctgg tcagtcagta gttccaaccg caccgagtct cacgcgtcgc
    20221 atagttcccg gagcttatga atatttggat tataacggtg gcactgtgaa tttggaatgg
    20281 aattcggatc catcagcgcg aagttataat ttatatcgcc aaatcgggga tggttcgttt
    20341 gagttgattg attctgatat tttttcactt atagtcagtg atgcgacggg tatgcttgta
    20401 actcctgcgg taaacgatcc actctcggcg attacggtta aatatcaggt ttgtgcggta
    20461 agcaaagatt tagtagaagg acctccgagt aataccatta cgattagtga tgatattagc
    20521 ccgcaactaa ttggaacatc ggtgatcaat cttgcttctc aacgatatca attcaatttt
    20581 cgtttttcag aaccgctggt aatcagctct gccgaagatc taaataatta tacaattcag
    20641 gatcctgaca cagtagtatt caccaaggaa cgagccgcct atatgggtta taattctact
    20701 aataatgatt atgaagtatt attaacggtt tcaatttcca acggtgaggt tttgcctcgt
    20761 ggattctcag taatacttgg caacgagata gtagatcttg ccggaagaag tattaatagc
    20821 tctgccaata cgtatgttta ttcatctcca ccaaccccag ttttgttttc acctcctgat
    20881 aactcgacta atatttcatc ctctcaggta ttgacgtgga aaatatcgaa tggtgcatta
    20941 tcttatcgtc ttcagatttc gactaatgtc aattttacct caacgatatt tgatagtgat
    21001 aagattacgt caacctcttt tagcttagat gcagtatcag ggttggtaag cggtgcgaaa
    21061 tactactggc gggtacaagc ggctaactcg gtcggaagta gtgcattttc tactacttgg
    21121 agttttacct tacagtaaat tcactatttg atcaagataa aagggcgggt ttatataatc
    21181 cgcccttttt cgtgataata gtagagaaat aaggtttaca agaagatcta acccgatttt
    21241 gaattgccgt tatttagcaa gaaacgatgg ctaattataa ttaaaattga tattcgaaat
    21301 ttgttaaacc tctctgaggt agcaatttct ggcgattatg tttttctaca aaacttaaag
    21361 ccctacgggc tagttgg
//
LOCUS       DAOMJU010000031        20892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2375_length_20892_cov_24.547392, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000031 DAOMJU010000000
VERSION     DAOMJU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 20892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20892
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2375_length_20892_cov_24.547392"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLO58_04585"
     CDS             <1..277
                     /locus_tag="PLO58_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HQO74099.1"
                     /translation="YRALSDPTRRQILKLLREKDLTAGEIAEHFTMTKPSLSKHFNIL
                     RSAGLIQGEKSGVTITYHLNVSVLEEAIMALMEIFKLKGENNGRQSE"
     gene            258..947
                     /locus_tag="PLO58_04590"
     CDS             258..947
                     /locus_tag="PLO58_04590"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HQO74100.1"
                     /translation="MEDNRNKIELMRPAITITIIAVIMMLIASAISWQKLPNDVRVPV
                     HWNIAGKADRYGSKFQGLMLMPLFTIFLAVCMVVVPLIDPHYRHIKSSMKAYNIILSA
                     IALLMTFLHFLILMATFGQPIDIMKNVCLAIGLLFILIGNYTPKIRSNYFLGIRTPWT
                     LSSELSWHKTHCFAGRLFILLGALIGLGGLTLPGIAVFVILLVGVIGLLLSVTIYSYV
                     IWQKDPEKRKV"
     gene            complement(1015..4419)
                     /locus_tag="PLO58_04595"
     CDS             complement(1015..4419)
                     /locus_tag="PLO58_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQO74101.1"
                     /translation="MLKLVYMTDLTCEREQFIRNITENQFVCAAAGTGKTTVLVRRYL
                     EILLSGAADCDQIIAITFTDKAANEMKERVRSSLLKNEAGLEPAKIASLIERLNTAPI
                     STVHGFCLRILRDNIGELPFDPLFKICDETAEALLRDQFWQKFLERKIAERDPDLTIL
                     INSITLAELSKLLDLIYEKQAECAPILERYKNLNVTSHLADIEKACRDYNFQLLYEYF
                     SDTTISDIINQISTFKSSKSDDVLQRDFCIILTASAEVLGRRQIPVSVRDGSLRKALG
                     GGKKGTAAAWGNELETVREFHSQLAAKFRLIKDEFHTFDPEIEKLHADVMQSLAHLAL
                     DFLQEYRAEMRRKALLDFTGIELETENLLARRSPEIVRYIRNFKHLLVDEFQDINPIQ
                     YRIIQLLQELNPDLITFFVGDEKQSIYRFRGAEVEIFNTLREQSDVQPLSINYRSVQA
                     LMNFYNFFFAQFLGSTPPAERYAAHYPLPIAAKDNSISTEPPVELLVIEEDEEFAARP
                     RQESPKDEVLEAQAIANRIWQLHKAEIIRENGIIREAEFGDMTILLRSRTHQSQFTEA
                     LRQAGIPFYVVTGIGFYDQPEIVDLINYLRVLLNWHDEVALVGTLRSPLVGLNDATLT
                     ELAREESLWKGINQYLKNGKEFVLEADEAARLKNFYRNYTELNKQITQLSTAEIINQI
                     VELTNFTALLAGLPDGTQKIANVKKLIDLALEWEKSESLSPIDFIRRIRIYRDKEVRE
                     GEANLSAAKGDAVTIMTIHAAKGLDSQIVFLPLLADKINYRSDRFLFHSDIGAAVAIK
                     ADKNQPHPFHYNQLKHLERRRTLAEEKRLLYVAMTRARSYLVCSATVSGKSNEPDKSL
                     WDLSAEIFVRAHEKNLCRIVKLTKTEIEQTALPSGISLVQPVELNDETLRRIRTQIQP
                     LEFKPPIRNLTATALAEWVVAGEKIASTAEPEADQALSPQELGTLIHKAFSWWDFQSL
                     DSFQNMLADLLRPYRLTAPESQKISAELSDWVKQLLDSDNHLRQRLTSARNRRREVEV
                     VGLYGEMILEGKIDLLIENNDNTLTIIDFKSDRIAEKPDEVLLKKYSTQLDFYAFILE
                     RSSGRKVREQALYFIRNGLLITKTITESVLDEAEQNIRKYISELAK"
     gene            4487..5995
                     /locus_tag="PLO58_04600"
     CDS             4487..5995
                     /locus_tag="PLO58_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HQO74102.1"
                     /translation="MPIIIPFSSEWITFACFLGLILGIVGLSEVLRRSSRLTGEQTRR
                     FVHIGVGIFVIFSPLFFKSALQPALLALIFILLNAVALKAGQMKGMHTTERRSYGTVF
                     FPLTFLILIAMYWYSDPVILMLGMMVMTLADPLASIVGMKVKKPRQFTFWKDPKSVQG
                     SLVVFITTFIIITAFLPLFRWLDGTPIPNWQSLIVMALTVGVVATLAEAISSAGSDNL
                     SLPLSAALMIDVMQSLTFQGQIAVMGWVLFSFLLAYTAYRLKALTAGGAAGAMFLGSV
                     VFSIGGINWVIPMATFFVLSSILSKLGKAHKSQLAIMQEKGSNRDLIQVYANGGVALV
                     MAILYFYTEMDLFYLMFLGSLAAATADTWGTEIGTFSKSQPVHILTGKAVPAGTSGGV
                     TVLGTSGVFVGAGILTLSGIFPLEQSLMKIALIIIISGILGALIDSVLGGTIQAQYRC
                     PVCRKTTEKVKHCNNQATTLISGYRWINNDVVNLVCTLSGALLVGVGCWLLM"
     gene            complement(5951..>8620)
                     /locus_tag="PLO58_04605"
     CDS             complement(5951..>8620)
                     /locus_tag="PLO58_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQO74103.1"
                     /translation="ESLTIALELLKNKSNDKLGELLVIDGFYEFTPIQHKILQALTPL
                     FTKIICTSIFDSSHPIYEYCQNRPGWLGSGETVMLSERTISSDPLRFLNSKLFKELIK
                     HDNFTPPNAVSNWKNEWNTPVKIVQCPSRRSEIETAARTIKRWLSEGMNPAKIGITFR
                     GSYDYRSLIKLIFPNFGIPVAMEVQNLFLSEPAQLITRIVNLNRENFSRPVFIDLLRL
                     PVLQRFYGADLLQELEIYSTEWGLPSAKSDWTARCRQQIEYLQFVLQIDREENDRERR
                     DAQLQIKHLQAIQPILTRLIEELSLPESAEWSEYHYLLIKILERYYPPDQPDEAQKIL
                     PIKSVLKRLVRLRDSHTKTSLEELAGILPHFLDTGENEATLSSSGILCANLMDARAWD
                     VDGLILLGLIDGEFPAIHPENPLFRKDLRSRLNQLANQPIFAEAAFDLAEEKFLLYLL
                     TTRVNQRLLITYPESDNKGRALPVSPFIDEILACQPANTNQKIVEWEAIPAGQVLPDS
                     INCSSDSDLLQIVLAEKTAREDIEKLVSLIDRKIFDELVINLNCERRRLAGDGIRNGV
                     LPESRFYPEFLAKPLSTTKIQNYVDCPFAYLCAHIWNIEISREPQIGLDALTEGLLIH
                     KVLEQFVQDFLSGEPQSWSDFLEVADDSFLNKLIVQINALFRPKLYFVPDLLWEQDLN
                     HLKVGLRNFVEEERRYCPTGFAPILTEKMYALDDAFLIGNDPTLPVTITGKIDRIDHN
                     ASGERFLVIDYKRSSSGIIDIVNGAQSGAQFQIPLYLLILLKHRPNEQIGGAFYYSFK
                     DGKRTRGFLVDVKFERTSELGLSDLNELLNKTQENVTAILEQIYRGNFNLLRRNENRC
                     RENECDFYDLCRIASGQKNSHQQPATHSHQKRT"
     assembly_gap    8621..8720
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8721..9279)
                     /locus_tag="PLO58_04610"
     CDS             complement(<8721..9279)
                     /locus_tag="PLO58_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74104.1"
                     /translation="MTQIKYITGPLKSGKTAKVIQETVDVLRSGQVVGGILPSRDHID
                     YIKREILRSQPVLAPGQLFLGTFYAWAERILDECHYNFQTVPSAEEWLNLHDILKQSP
                     VSNYHSFSGYIQILQDIFKDFRESGLTSAELELLAKNSGERELIEWVRLFNKLRQNYR
                     RSSAGPSGESLTIALELLKNKSNDKL"
     gene            complement(9425..11239)
                     /locus_tag="PLO58_04615"
     CDS             complement(9425..11239)
                     /locus_tag="PLO58_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HQO74105.1"
                     /translation="MFKNHPKGLVVAFFTNMGERFGFYTMMAILVLFLQAKYGMNEST
                     AGNIYSWFYFSIYALALLGGILADSTKKYKTVILFGQIIMFAGYFLLAIPGLSLTISL
                     IGLFTIALGNGLFKGNLQAVVGQMYDNEQYSKIRDSAFMIFYMGINIGAFFAPFAATG
                     IRNWWLKVNHFLHDGSLPAICHQYINGTLKDVSELQTLANRVSLSGPVTDLSAFAHDY
                     LEVFSTGYNYAFGIAAVAMIISLLIYVVFNRHLPNKEKVSAEKNSAIALNIQPMTVLI
                     ALLAGAVVAFLVSLVIDIGTGAAIGLFVAFVTWILIVSKKEEKPRVTALLLVFFVVIF
                     FWMSFQQNGLTQTFFARDYTVKTVDRITYMFFTLENILTVIAFVAGLVLVFGRKRSRR
                     TRLIGGIILVVSGVIGYYLFQQGSDQNPISPEIFQSFNPLFIVAFTFVVMGVFNWLNK
                     KGKEPSTPRKIGIGMIVAAIGFSVLLIGSLNLVSPHELKFVDESGVIKYSPVPDYSRV
                     SPYWLIASYLILTIAELLLSPMGLSFVSKVAPERFQGLMQGGWLLATAVGSKLVFVGS
                     TFWGILDLWQLWLIFVVCCLLSAAFIFSIMKRLEKATS"
     assembly_gap    11556..11655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11931..12200
                     /locus_tag="PLO58_04620"
     CDS             11931..12200
                     /locus_tag="PLO58_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQO74106.1"
                     /translation="MESSKLYVGNLKYSVTDEQLAQLFAQFGEVKSANVIKGKGFGFV
                     EMGSIEEANKAHDALDGKEFEGRTIRIADAHPPRTEKKETHEKQE"
     gene            12377..12913
                     /locus_tag="PLO58_04625"
     CDS             12377..12913
                     /locus_tag="PLO58_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQO74107.1"
                     /translation="MAKQNVNHPVYDEQAFNNLVRENHQRIYGLFRRMVGSQAEADDL
                     TQETFIKVYKNLPKFRQESSIQTWIYRIAINQGLNYLRRMKIRQTLGLELADSAAEPD
                     EFQSNQSNRNLLRRAIAKLPPRQQMVIILRSFQELSFKEIAAVLNITENAAKVNFSHA
                     VKNLREIFLKMGVTYENL"
     gene            12900..13502
                     /locus_tag="PLO58_04630"
     CDS             12900..13502
                     /locus_tag="PLO58_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HQO74108.1"
                     /translation="MKICKQIKDYLPLFFTGDLNESAKRLVENHLKDCPACRKFSYEI
                     QHLIDDLQPDPVNIEPNYGAELVVKIQNRLHNERRPKKLKYYLVPVLGSILIFIIIGF
                     NLMRNQSISSQWLSNDNNIDLYMDLTHSGYFSEMPEDVFDFVNFNENDLITSELRQKI
                     SSVVLSTNSNLLIDDYIESTAFLTDQDFESVLEEIEKSIL"
     gene            13522..14028
                     /locus_tag="PLO58_04635"
     CDS             13522..14028
                     /locus_tag="PLO58_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74109.1"
                     /translation="MKNNWLLSMSLILLGFTGQLAAQPHSFDESKISMAERREKMETL
                     RIYKMTEFLELTPEQSEKFFPRLKQYEKNVRKMQHQQMKLVREIDQITQNPDTTITES
                     DVKKYLRAMAQIEKDIIAEKEKFIGGLSSILTPSQQLKYMIFDNRFRRHLMKTLNPPP
                     NEMKNQQK"
     gene            14038..14616
                     /locus_tag="PLO58_04640"
     CDS             14038..14616
                     /locus_tag="PLO58_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HQO74110.1"
                     /translation="MKKYFILGLILLTVPALILAQPEVKGKAEPCQQALKGSHEMHCQ
                     GQCKTPLADLLKLTDQQKVDHKKIMVKYQRLNIPLQADLKLARLDLKEALDNLDQKKI
                     DESVKKINDIKAKLFKNKIDQRVEFLKTLTDEQRKILKEQPCGMQKVKKIKRLEGRFG
                     WDDMGKLGSEIDELLGLVMDIDVGEMEIEPED"
     gene            14621..15451
                     /locus_tag="PLO58_04645"
     CDS             14621..15451
                     /locus_tag="PLO58_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74111.1"
                     /translation="MIEQILNWMKHVEIGEGDLTTESIAPYPLHSSGRLVMSSDTLFS
                     GRAIMEKLLESAGEQLQIEWHFDEGDEIPAGATIFKFNGNGAEILKIRRLLEWLASSL
                     SGLATATREVVKFLGDHNKKLVAGNSVSPIFEEFEKIAFTTGGGEMPFSGISQRIYLT
                     QNHFIYAGKSPDEVITTLVDELGVARKKIKIEVEVNDPIQFEEINKLDCDVIHLVDFN
                     DEQLRTVFEKLNPRHKTVVHLATLADFRPEYDDYFFKYVAVEDLHRTRHYSPARLIFS
                     "
     gene            complement(15637..16560)
                     /locus_tag="PLO58_04650"
     CDS             complement(15637..16560)
                     /locus_tag="PLO58_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQO74112.1"
                     /translation="MKNIVLSQDKFIPSQSGANITFHRLAWDNSSQPESIIAGSDLRY
                     YSLASDNTALHSHEFAEFFIVISGKVRHIVNGETQDLQAGMLTFIRPNDVHSFEKLND
                     SVCEMVNVAFKLELLLDLSTYLGNDFFLRRYTGPVLPPTFTLSLVETEELALRLIRLI
                     TLQKTDLDQARLKLKMILAEIFTRFFLEWQTLDPSRPQWFDKLCSEMKRKENLIGGLK
                     TMLKLAPCTQQHLCKCFRQYLNTSPTEFINEQRIKLAAKQIIETDEKILAIALEYGFK
                     SLSRFYKIFHKYYGVSPARLRQISRQKVIPL"
     gene            16663..>17333
                     /locus_tag="PLO58_04655"
     CDS             16663..>17333
                     /locus_tag="PLO58_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQO74113.1"
                     /translation="MENNSIQGLQLVRQAFNRQKTTRVPWVPFVGCHGGKLIGQTAEN
                     YLKSGKLIAEGLRKANELYRPDGLPVIFDLQLEAEVLGCELLWADDAPPSVITHPLDS
                     LNGKNLSDLPEFSLAKGRYPEVAQAMDIIRQDFAKDLAIYGLICGPFTLALHLMGNEI
                     FLEMYDHPEKVEELLAYCKEIAFQVSDFYLKNGADIIAVVDPMTSQISPEHFQRFITP
                     ALNPI"
     assembly_gap    17334..17433
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17434..17937
                     /locus_tag="PLO58_04660"
     CDS             <17434..17937
                     /locus_tag="PLO58_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQO74114.1"
                     /translation="LEAIRNIDEGGQVGFILAPGCDIPYNVPPQNLQAVAEMVYDDYQ
                     REVARRTLTAREFEVDWIVLPDYANREEVILDIITLDSATCAPCKYMVEAALKATADL
                     SIKTTVREHKITTSEGIGMMKKLGVRSLPTICIDGEVKFSSIIPDQQTLKAAILDRWK
                     FKTGQDK"
     gene            17934..18875
                     /locus_tag="PLO58_04665"
     CDS             17934..18875
                     /locus_tag="PLO58_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQO74115.1"
                     /translation="MIPIRLVTGFLGSGKTTLLKNLIRQNQSRRLVFLVNEFSPTDID
                     GSLIANLTPDYLSIPGGSIFCRCLAGKFIEYLQEIPQRFTQDGNEIDELVIEASGVAD
                     PRVIFKMLSETGLDKVYYIREVLNIVDPASLGKLFHTLTSIRAQIEAADIILINKVDI
                     ASPEAIAQTESLIRQVNPSAYVYRTTFSQLPKDLPENPQRLQVPEGEFAPCRDPRYAT
                     IILNSGDMNYEQLRQAITPVADDVYRLKGYLFDSHQYLYVDYSPAGWQIEPASSIEQE
                     PQIAIIINGVNQAKVIEHFKLCGLLENNVYFGAKNGT"
     gene            18865..19494
                     /locus_tag="PLO58_04670"
     CDS             18865..19494
                     /locus_tag="PLO58_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HQO74116.1"
                     /translation="MARNEELYLAILKGDRAKVQAILQNAINLNQDVNELLEETMIPA
                     MREVGERFARNEIYVPEMLISARAMQAGLTLIEPILAKSNHKARGKVAIGTVKGDLHD
                     IGKNLVSIMLKGAGYEVLDLGVNCSVEKYAEAVNAGINILLCSALLTTTMMYMKEIVD
                     YFKSNNAVKIVVGGAPVTQSFADMIGAHAYGQNANDAVRIVANLTGAVV"
     gene            19522..20238
                     /locus_tag="PLO58_04675"
     CDS             19522..20238
                     /locus_tag="PLO58_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQO74117.1"
                     /translation="MHTRHLFLLIGQSNMAGRGEMDRVLPISDDRIQMFRNNRWQKAV
                     EPLHDDRADLAGVGLGMSFASSILKRNLVQNVGLLPCAVGSTAIEDWLPGTPHYQRAV
                     AVIKTALATDTYENMLGAILWHQGEDDAKDKILAEKYRDRIVSLVWSFRRDLNMPSLP
                     FISGELGDFINAATGYPYGPDISAVLKELESILPDYACVSAEGLASKDDGLHFNAPSL
                     REFGKRYALAYQRLLSYRLN"
     gene            complement(20266..20736)
                     /locus_tag="PLO58_04680"
     CDS             complement(20266..20736)
                     /locus_tag="PLO58_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74118.1"
                     /translation="MKIFDNKGMLIIPNPMRGKFTEKPKLLVVNQLFCPNGHNLINQR
                     VTFNGYPGILIKVRQDEAIGLIALSPFYGEHSRFSLDIDLIDGKLLKLICPTCDVEMP
                     IIAQCECGGNLAAFFLTRECNFNDCVGICTRVNCHNSRIVHSGELITGSMLESL"
BASE COUNT         5856 a         4346 c         4617 g         5773 t
ORIGIN      
        1 ctatcgggcg ctatccgatc caacccggcg acaaattctg aaattactgc gcgagaaaga
       61 cctgaccgct ggtgaaatcg ccgaacattt taccatgact aaaccctctc tgtcaaagca
      121 ctttaacatt ttacggtcag ccggactgat tcaaggtgaa aaatcggggg ttacaattac
      181 ttaccatttg aatgtcagtg tacttgaaga agccattatg gcattgatgg aaatatttaa
      241 actcaaaggg gaaaataatg gaagacaatc ggaataaaat cgagcttatg cgaccggcca
      301 taaccattac gattatcgca gtaattatga tgttaattgc ctccgctatc tcttggcaga
      361 aattacctaa cgatgttcgc gttcctgtcc attggaatat cgccggtaaa gccgatcgtt
      421 atggcagtaa atttcaagga ctgatgttga tgccgctttt cacgattttt ctggcagttt
      481 gtatggttgt agtaccattg attgatccgc attatcggca tatcaaatca tctatgaagg
      541 catacaacat aattctgtca gccattgcat tgctaatgac atttctgcat ttcttgatat
      601 taatggctac gttcgggcaa ccgatagaca ttatgaaaaa cgtgtgttta gctattggac
      661 tattatttat tctaatcggt aattataccc ctaaaattcg tagtaattat ttcctcggca
      721 tacgaactcc atggacttta tccagtgaat tgtcctggca caagactcac tgttttgcgg
      781 ggcgactctt tattttactg ggggcgctga ttggcttagg cggacttact ttgcctggca
      841 tagcggtttt cgttattctg ctagttggag taatcggact gcttctatct gtaacgattt
      901 attcctatgt aatctggcaa aaagatcccg agaagcggaa ggtataaacc gcaaaacgca
      961 tcaagaagat caccttttta ttttcttgga ggtaacttga gtcgttaaaa atacttactt
     1021 tgccaactcg ctgatatatt tgcgaatatt ctgttcggct tcgtctaata ccgactcggt
     1081 tattgtttta gtaatcaata aaccgttgcg aatgaaataa agagcctgtt ctctaacttt
     1141 ccgcccagaa ctgcgttcga gaatgaaagc gtaaaaatcc agctgggtgg agtacttttt
     1201 caataaaact tcgtccggtt tctctgcaat gcggtcggat ttgaagtcta taatagtaag
     1261 agtattgtcg ttattttcga tgagtagatc aattttcccc tcgaggatca tttctccgta
     1321 aagcccgacc acctcgactt cccgtcgccg atttctggcg gaagtcaatc gctggcgtaa
     1381 atgattatca gaatcgagca attgcttaac ccagtcggaa agttcagcgc taattttctg
     1441 cgactcaggt gcggttagtc ggtagggacg cagtaaatca gccagcatat tttgaaacga
     1501 atccagcgac tgaaaatccc accagctgaa ggctttgtga atcaaagtgc ctaactcctg
     1561 tggtgaaagc gcctggtcgg cttccggctc ggcggtggag gcgattttct caccggcgac
     1621 tacccattct gccagagcgg tagcagttaa attccggatc ggcggtttga actcgagcgg
     1681 ttgaatttga gtgcgaattc tccgcaacgt ttcatcgttt aattcgactg gctgaacgag
     1741 tgaaatccca ctggggagag ccgtctgttc gatttcggtt ttggttaatt ttacgatgcg
     1801 gcacagattt ttttcatgcg ccctaacaaa aatttcggca cttaaatccc agagcgattt
     1861 atccggttca ttggatttgc cggacacagt tgccgagcag accaaatacg aacgggcgcg
     1921 agtcatagcg acgtacagca ggcgtttttc ttcggctaaa gtccggcggc gttcgagatg
     1981 ttttaattga ttatagtgga acggatgagg ttggttttta tcggctttaa tggcgaccgc
     2041 cgcaccgata tcagaatgaa aaaggaagcg atcgctccgg taattgattt tatcagccaa
     2101 cagaggcaga aagacgattt gactgtcaag accctttgcc gcgtgaatcg tcataatcgt
     2161 taccgcatca cctttggcgg cggatagatt ggcttcgcct tcgcgtactt ccttatctcg
     2221 ataaatgcga atacgccgga taaaatcaat cggcgagagg ctttcagatt tttcccattc
     2281 cagtgccaag tcaatcaatt tttttacgtt ggcaattttc tgagtgccgt ccggtaaacc
     2341 ggccagaagc gcggtaaaat tagtcagctc aacaatttga ttaattattt cggcggtgga
     2401 tagttgcgta atctgtttgt ttaattcagt ataattacga tagaaattct tcaggcgtgc
     2461 ggcttcatcg gcttccagta cgaactcttt tccatttttc aaatattggt taatgccttt
     2521 ccatagactt tcttcacggg ctaattcagt cagagtggcg tcattcaatc cgaccagcgg
     2581 cgaccgcaat gtccccacta aagcgacctc gtcgtgccaa ttcaataaca cccgcagata
     2641 attaatcaag tccacgattt ccggctggtc gtagaatccg attcccgtca ccacgtaaaa
     2701 agggattcca gcctggcgca gagcctctgt gaactgagat tggtgggtgc gcgaacgcag
     2761 gagaatcgtc atatcgccga attcagcctc gcggataatg ccgttttccc tgattatttc
     2821 ggctttatgt aattgccaaa tgcgattggc gatagcttgg gcttctagga cttcgtcttt
     2881 cggactttct tggcgcggac gcgcggcgaa ttcttcatct tcttcgatta caagcagttc
     2941 aaccggcggt tcggtagaaa tagaattgtc cttagccgca atcggtagcg ggtaatgcgc
     3001 ggcataacgt tccgccggtg gtgtgctacc gagaaattga gcaaagaaga aattgtagaa
     3061 attcatcaga gcttgaacgc tccgataatt aatcgaaagg ggctgaacat ctgactgctc
     3121 gcgcagagtg ttgaatatct cgacttccgc cccacgaaag cggtaaatgg attgcttttc
     3181 atcaccgacg aaaaaagtaa tcaaatcggg attgagttcc tgcagaagtt gaatgatgcg
     3241 atattgaatc ggattaatgt cctggaattc atcaaccagc aaatgtttga aattcctgat
     3301 gtaacgcaca atctccggcg accgccgcgc cagtaggttt tcggtttcga gctcgatgcc
     3361 ggtgaaatcc agcagagctt tgcgtcgcat ttccgcgcga tattcttgca gaaaatctaa
     3421 tgccaggtgc gccaggcttt gcattacatc agcgtgaagt ttttcaattt ccggatcgaa
     3481 agtatgaaat tcgtctttga ttagtctgaa ttttgcggct aactggctat ggaattcacg
     3541 aacggtttct aattcattgc cccaagccgc ggctgtgcct tttttcccac cgccgagcgc
     3601 tttacgcaaa gagccgtccc ggactgaaac cggtatctgt cgccgaccga gaacttcagc
     3661 ggaagcagtg aggataatac agaagtctct ttgcaaaacg tcatcagatt tggatgactt
     3721 aaaggtagaa atttgattta taatatcgct aattgttgta tcgctgaaat attcgtatag
     3781 caattggaaa ttgtaatcgc ggcaggcttt ttcaatatcg gcaagatgac tagttacatt
     3841 gagatttttg taacgttcca gaatcggcgc acattcggcc tgcttttcgt agatcaagtc
     3901 cagcaatttg gataattcgg cgagtgttat tgaattgatt aggatggtta ggtcaggatc
     3961 gcgctcggca attttacgct ccaggaattt ttgccaaaac tgatcgcgga ggagggcttc
     4021 cgcagtttcg tcacatatct tgaatagtgg gtcgaaaggc aattcgccaa tgttatcccg
     4081 cagaatacgg aggcaaaaac catgcacggt agaaatcggg gcggtattca ggcgctcgat
     4141 caaactggcg attttcgcag gttccaaacc ggcttcgttc ttgaggagag atgaacgcac
     4201 gcgctctttc atttcattag cggctttgtc ggtaaaggta atagcgatga tttggtcgca
     4261 gtcggcggct cccgaaagta aaatttccag gtagcgtctg accagaacgg tggtcttgcc
     4321 ggttccggcg gcggcgcaga cgaattgatt ctccgtaatg tttctgatga actgttcgcg
     4381 ttcgcaggtt aagtcggtca tatagactaa tttaagcatc aaagcttttg gaaataaatt
     4441 ttaaaaagca taaaattcga gcgtttttat ggggagaaaa gaataaatgc cgattatcat
     4501 accgttttct tcggaatgga ttactttcgc ctgctttctg ggtctgattt tgggaattgt
     4561 gggcttaagc gaagtcctgc gtcggagtag tcgcctgacc ggcgaacaga cccgccgatt
     4621 cgttcatatt ggtgtcggaa tttttgtgat tttcagtccc ttatttttca agtcggcttt
     4681 gcaaccggcg ttactggctt tgatatttat tttattgaat gccgtcgctc tgaaagccgg
     4741 acagatgaag ggaatgcaca cgactgagcg tcgttcttac ggtacggttt ttttcccgct
     4801 gacctttttg attttgattg cgatgtactg gtattctgac ccggtgattt taatgcttgg
     4861 gatgatggta atgactctgg cggatccgct ggcttcgata gtcggtatga aagtcaaaaa
     4921 accacgccaa ttcactttct ggaaagaccc gaaatcggtg cagggttctt tagtcgtttt
     4981 tattaccaca tttataatca taacagcgtt tctgccgctt tttcgctggc tggatggcac
     5041 gccgattccc aattggcaaa gtttgatcgt gatggcgtta accgttggtg tggttgctac
     5101 gctggctgag gcgatttcaa gtgctggttc cgataatttg agtttgccat tgagtgcggc
     5161 tttgatgatt gatgtgatgc agagtttgac ttttcagggg cagattgcgg taatgggctg
     5221 ggtactgttt tcatttttgc tggcttatac agcctatcgt ttaaaagcct tgaccgcagg
     5281 cggcgccgcc ggggcgatgt ttctcggctc ggtcgttttt tcaatcggcg ggattaactg
     5341 ggttatcccg atggcgactt ttttcgtgtt atcgtctatt ctctccaaac tcggcaaagc
     5401 ccacaagagc caattagcca ttatgcagga aaaaggtagt aaccgcgact taatccaagt
     5461 ttatgccaat ggcggggtgg cgctggttat ggcgatcttg tatttttata ctgaaatgga
     5521 tttattttat ttaatgtttt taggctcgtt agcggccgcg actgccgata cctggggcac
     5581 cgaaatcggc actttttcga aaagccaacc ggttcatata ttgaccggta aagcggttcc
     5641 ggctggtacc tctggtggtg tgacggtgtt gggcacttcc ggcgtattcg ttggtgccgg
     5701 tatattgaca ttgagcggaa ttttcccttt agaacaatcg ctgatgaaga ttgccctgat
     5761 tatcattata agtggaattc tcggagcgct gattgattcc gtattgggcg ggacgattca
     5821 ggcgcagtat cgctgtccgg tctgtcggaa aaccaccgaa aaagtcaaac actgtaataa
     5881 tcaagcgacc actctgatct caggttatcg ctggattaat aacgatgtcg tcaatttagt
     5941 ctgtacgctt tcaggtgcgc ttttggtggg agtgggttgc tggttgctga tgtgaatttt
     6001 tttgaccgga tgcaattcgg cacaaatcat agaaatcaca ctcattctcg cgacagcggt
     6061 tttcatttcg cctcaataga ttgaaatttc cccgataaat ttgttccagg atcgcggtaa
     6121 cattttcttg agttttattt agtaattcat tcagatcgga caatcccaat tccgacgtac
     6181 gttcaaattt gacgtcaacc agaaacccgc gcgtcctttt accatcttta aaggagtaat
     6241 aaaaagcgcc gccgatttgc tcgttaggcc tgtgtttcaa aagtatcagc aaataaagtg
     6301 gaatctggaa ttgggctccg ctttgcgcac cattgacaat atctataatg ccactgctgg
     6361 agcgcttgta gtcgatcacc aaaaagcgtt cgccagaagc attgtggtca atccggtcaa
     6421 tttttccggt tattgtcacg ggtaatgtcg ggtcatttcc aatcaagaaa gcgtcatcca
     6481 gcgcatacat tttttcggta agaatcgggg caaagccggt cgggcagtaa cgccgctcct
     6541 cttcaacgaa attgcgtaat cctactttta aatgattcaa atcctgttcc cataacaaat
     6601 cgggaacgaa atataacttc ggtcggaaaa gtgcatttat ctggacaatt aatttattta
     6661 gaaaactgtc gtcggctacc tcgagaaaat ccgaccaact ttgcggttcg ccagacagaa
     6721 aatcctggac gaactgctcc agaaccttat gaatcaaaag tccttcggtc agagcatcca
     6781 gcccgatctg gggttcgcgc gaaatttcga tattccaaat gtgagcgcat aaataggcaa
     6841 acgggcaatc aacatagttc tggattttgg tggtcgaaag cggctttgcc agaaattccg
     6901 ggtaaaacct tgactccggc aaaacgccgt tccgaatacc gtcgcctgcc aatcgccgac
     6961 gttcacaatt aaggttaatt actaactcat cgaaaatttt cctgtcaatc aggctaacga
     7021 gtttttcaat gtcttcgcgg gcagtttttt cagccagcac aatttggaga agatcactgt
     7081 ctgaactaca attaatagaa tccgggagaa cctgtccagc tggaattgct tcccattcta
     7141 cgattttttg gttcgtattc gccggttggc aggctaatat ttcatcaatg aatggcgaaa
     7201 ccggtaaagc acgtccctta ttgtccgatt ccggataagt aatcaatagc ctctgattaa
     7261 cgcgcgtggt aaggaggtat aatagaaact tttcctcagc taagtcaaag gcggcttcag
     7321 cgaaaattgg ttgattagct aactgattca gtcgcgatct taaatccttg cggaataacg
     7381 ggttctcagg atgaatagcc gggaactcgc cgtctattag tcccagtaaa atcagcccgt
     7441 ccacatccca agcccgcgca tccatcaaat tagcacaaag aattccagac gaagaaagcg
     7501 tcgcctcatt ttctccagta tcgagaaaat gcggtaaaat tccggctaat tcctccagac
     7561 tggtcttggt atgagaatct ctgaggcgga ctaatcgttt caaaactgac tttatcggta
     7621 gaatcttctg cgcctcatcc ggctggtctg gcgggtaata acgctccaat attttaatta
     7681 ataggtaatg atactcgctc cactcggcgc tttccggcaa actcagttcc tcaattaaac
     7741 gagtcaggat gggctgaatc gcctgtaagt gcttgatctg taactgggcg tcgcggcgct
     7801 ctctgtcgtt ctcttcccga tcaatttgca gaacgaactg gagatattcg atctgctggc
     7861 gacatcgagc tgtccagtcg gatttagcag atggcaaacc ccattcggtc gaataaattt
     7921 ccaattcctg caataaatcc gcgccgtaaa aacgttgtag gaccggcagg cgcagtaggt
     7981 caataaagac cgggcgactg aagttttccc gattgagatt gacgatgcga gttattaatt
     8041 gagccggttc gctgaggaat aggttctgga cttccattgc caccggaatt ccaaaattcg
     8101 ggaaaattaa tttgattaag cttcggtagt cgtaactacc ccgaaatgta attccgattt
     8161 tggcgggatt cattccttct gacaaccagc gtttgatcgt gcgagcggca gtctcgattt
     8221 ctgacctccg actgggacac tgaacgattt ttaccggcgt gttccattca tttttccaat
     8281 tgcttacggc gtttggtggc gtgaaattat cgtgttttat taattccttg aaaagttttg
     8341 aattcaggaa tcttagcgga tcagacgaaa ttgtccgttc tgaaagcatg accgtttcgc
     8401 ccgaacccaa ccagccggga cgattctggc aatattcata aattggatgg gaggaatcga
     8461 aaatgctggt gcagattatt ttggtaaata agggcgtcaa agcttgcagg attttatgct
     8521 gaatcggagt gaattcgtaa aagccgtcaa ttaccagtaa ctcacctaac ttgtcattcg
     8581 acttattttt aagtagttca agggcaatcg tgagcgattc nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn ctaacttgtc attcgactta tttttaagta gttcaagggc
     8761 aatcgtgagc gattcgcctg aaggtccggc actactccga cgatagtttt gccgtaattt
     8821 attaaataac cggacccatt cgatcaactc tcgttcgccg gaatttttgg ctaataattc
     8881 caattctgcc gatgtcaatc cgctttcacg aaaatctttg aaaatgtcct gcaaaatctg
     8941 aatgtagccg gaaaatgagt gataattact cactggtgat tgttttaaaa tgtcgtggag
     9001 attgagccat tcttcagcag atggaacagt ttgaaaattg taatgacact cgtcgagaat
     9061 ccgctctgcc caggcgtaga aagtacctaa aaatagctga ccgggcgcta aaaccggctg
     9121 actacgcaaa atttcccttt ttatataatc aatgtgatcg cgtgaaggta gaatcccacc
     9181 cactacctgt ccggagcgga gaacatcaac cgtttcctga ataaccttgg cggttttgcc
     9241 ggatttcagc ggaccagtga tatatttaat ttgcgtcata tgattaatct caaatccttt
     9301 tcagccccaa cgaatgtaat tcaaaacgcg attatttgcc agaatttgtc gccaacttac
     9361 tgccgttttg cgggaatgtt gcaaagcggc cgaagttgca gctgaaattt ttacaaatag
     9421 aagtttagct agtcgctttc tccagtcgct tcataataga gaatataaaa gcggctgaaa
     9481 gcagacagca aaccacgaaa atcaaccaca gttgccagag gtcgagaatg ccccagaaag
     9541 tacttccgac gaaaacaagt ttactgccga ccgccgtcgc cagaagccag ccaccttgca
     9601 tcagtccctg aaaacgttcc ggtgcgactt tggacacgaa agatagcccc atcggactca
     9661 gtaataattc cgcaatggta aggattaagt aagatgcaat cagccagtag ggcgagactc
     9721 gggaataatc cggcaccggg ctgtatttaa taacaccgga ttcgtcaacg aatttcaatt
     9781 cgtgcggtga aaccaaattc agcgagccaa ttagcagtac gctgaaaccg atcgcggcaa
     9841 cgatcattcc gatgccgatt tttcgcggtg tggaaggctc cttgcccttt ttattgagcc
     9901 agttgaaaac gcccattacc acaaaagtga atgctacaat gaaaagcggg ttgaaagact
     9961 ggaaaatttc cggcgaaatc ggattctggt cactgccttg ctggaaaaga taatatccga
    10021 taactccgct gacaaccaaa ataattccac ctatcagacg agtccggcga ctgcgcttac
    10081 ggccaaatac caaaaccagt ccggctacga aggcaattac cgtcagtata ttttccaatg
    10141 tgaagaacat ataagttatc cgatcgactg ttttgaccgt atagtcgcgg gcaaaaaatg
    10201 tctgcgtcaa gccgttttgc tgaaaagaca tccagaaaaa tatcactacg aaaaaaacca
    10261 gtagcagggc cgtaactcgc ggcttttctt cttttttcga aacaattaga atccaagtca
    10321 caaacgccac aaatagcccg atggcggctc cggtgccaat gtcaattacc aggcttacca
    10381 gaaatgccac tacggctccc gctaataagg cgattaaaac cgtcattggc tgaatattta
    10441 acgctatcgc tgaatttttt tcggccgaca ccttttcttt attgggcaaa tgccgattaa
    10501 aaacgacata aatcagcaac gaaataatca tagccactgc ggcaattcca aaagcgtaat
    10561 tgtatcctgt agaaaaaact tccagataat catgagcaaa agcggaaagg tcggtcaccg
    10621 gaccactcaa actcacccga ttggcaagag tttgcaattc actcacatct tttaaggttc
    10681 cattgatgta ttgatgacaa atcgcaggca aactgccgtc gtgcaaaaag tggttgactt
    10741 tcaaccacca gttgcgtatt ccagtcgccg caaaaggagc aaagaacgcc ccgatattaa
    10801 tccccatata gaaaatcata aaagccgaat ccctgatctt cgagtattgc tcgttgtcgt
    10861 acatctgacc gacgaccgct tgcagatttc ctttgaaaag accattcccc agagcaatag
    10921 taaaaagccc aatgagactt atcgttaatg atagcccggg aatagcgagt aaaaaatatc
    10981 cggcaaacat aataatttgt ccgaagagaa tcaccgtttt atatttcttg gtcgaatcag
    11041 cgagtatgcc gcccaataat gccagcgcgt aaatgctgaa ataaaaccag ctatagatat
    11101 tgccagcggt actttcattc ataccgtatt tagcctgaag gaataaaacc agaatcgcca
    11161 tcatcgtata gaagccgaac ctttcaccca tattggtaaa aaaggcgaca accagtcctt
    11221 tcggatgatt cttgaacatt gcgaaaacct cctcttagat gaaatatttt aaatatttag
    11281 tcataagata gtgacggaaa tataggtgta aaggcggaaa aataaaatga aaatttaata
    11341 gcgaatttcc caccttaggt taacaagaat ggcgaaaaaa atatgccaat agaaaataat
    11401 cttgtaaaaa cgggttatgt tttactagta gaagtgaaaa ccttgtcaaa gttgaatcaa
    11461 tatcgtttgc gatatttaga aaatggcgat cattttcaga tagaatacca attttacctg
    11521 tttattttct aataaaaatc cagttaattg cagatnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnntagaa taccaatttt acctgtttat tttctaataa aaatccagtt
    11701 aattgcagat tttctttgaa ttgcagaatt tttgttatta aaataatgcg ttatttggaa
    11761 agattgattt agaattagct atttatacca aattagatct tcatcctgcg cgagggagat
    11821 actcataggt gtcaaataat gtaaccttgt cggggttagc ggtgtaatca cttcgacctc
    11881 tacgttaatc tacattatct ctgacacttg aatttattat ggaggtttga atggaaagca
    11941 gtaaactgta tgttggcaat ctgaaatatt ctgttaccga cgagcaatta gcccagctat
    12001 ttgcccaatt cggggaagtc aaaagtgcca acgtaattaa aggcaaaggc ttcggttttg
    12061 tcgaaatggg ttcgattgaa gaagccaata aggcgcacga cgctttggac ggtaaagagt
    12121 tcgaaggtcg gactatccgt attgctgatg cccatccacc gcggactgaa aaaaaagaaa
    12181 cgcacgaaaa acaagaatag tttagctctt cttatcgaat ttcaataagg ttcagattcc
    12241 ggatttttcc ggaaaagctg atctttttat caaacgccat aatcgttcga taaatatctt
    12301 tttttatcta aaaatttatg tttaaccttt tacttgcgtt ttttgtctaa cgcaacgtga
    12361 aaaggaatta tacaatttgg ctaaacaaaa cgttaaccac ccagtctatg atgaacaggc
    12421 gtttaacaat ttggtacgtg aaaatcacca gcgcatttat ggactattcc ggcgcatggt
    12481 gggcagtcaa gccgaggcgg acgatttgac ccaggaaact ttcataaaag tttataaaaa
    12541 cttacccaaa ttcaggcagg aatcttctat tcaaacctgg atttaccgga ttgccatcaa
    12601 tcaagggctg aattacttga gacggatgaa aatcaggcaa actctgggac tcgagttagc
    12661 tgactcagcc gccgaacccg atgaattcca aagtaatcaa tccaatcgta atcttttacg
    12721 tcgggcaatc gccaaactgc caccgcgcca gcaaatggtt atcattctgc gctcttttca
    12781 ggaattatca tttaaggaaa ttgcggctgt tttgaacata accgaaaacg ccgccaaggt
    12841 gaatttttca catgctgtta aaaacctgcg agagatattt ctcaaaatgg gagtgactta
    12901 tgaaaatttg taaacaaatt aaagattatt tgccgctatt ttttaccggc gacttgaatg
    12961 aatcagccaa aagactcgtc gaaaaccacc taaaagattg tcctgcttgc cgcaaattca
    13021 gctatgaaat acaacacctg atagatgatt tacagcctga tcctgtcaat attgagccaa
    13081 attatggagc tgaattagta gtaaaaatcc aaaaccgact ccataatgaa cggcgtccta
    13141 aaaagttaaa gtactatctg gtaccggtcc tcggttcaat attaattttt atcattatcg
    13201 gatttaactt gatgcgtaat caatccattt cctcgcaatg gttatccaat gataacaaca
    13261 tcgaccttta tatggatttg acccattccg gttattttag cgaaatgccg gaagatgttt
    13321 ttgactttgt gaactttaac gagaatgatc tgattaccag cgaattacgg cagaaaataa
    13381 gtagcgtggt tctatctacc aatagtaact tactaattga cgactatatt gaatccactg
    13441 cctttttgac cgatcaggat ttcgaatcag tcctagaaga aattgaaaaa tccatattat
    13501 aaatttgcgg gaggatatca aatgaaaaac aattggttat tgtcaatgtc attaatacta
    13561 ctgggtttta ccggtcaatt ggcggctcag ccgcattcct tcgacgaatc gaaaattagt
    13621 atggcggaaa gacgcgaaaa aatggaaact ctccgtatct ataaaatgac cgagtttttg
    13681 gaattgacac cggagcagtc cgaaaaattt ttcccgaggt tgaaacagta cgaaaaaaac
    13741 gttcggaaaa tgcaacatca acagatgaag ctggttcggg aaatagatca aattactcaa
    13801 aatcccgata ctactattac tgaatccgat gttaagaaat atttacgtgc gatggcccaa
    13861 attgagaaag acattatagc cgaaaaagag aaattcatcg gcgggttgag ttcgatttta
    13921 acccctagcc aacagttgaa atatatgatt ttcgataatc gtttccgacg acatttaatg
    13981 aaaaccctca atcccccacc taatgaaatg aaaaaccaac aaaaatagga ggtataaatg
    14041 aaaaagtact tcattttagg tttgatccta ttaactgttc cggctctgat tttagctcaa
    14101 ccggaagtta aaggtaaagc cgaaccctgt cagcaagccc tgaaaggttc gcatgaaatg
    14161 cattgccagg gacaatgtaa aacgccgctg gctgatctac tcaaactgac tgaccagcag
    14221 aaagttgacc ataaaaaaat tatggttaaa tatcagcgct tgaacatccc tttgcaagcc
    14281 gatttaaagc tagctcgtct ggatttaaaa gaggctcttg ataatctcga tcaaaagaaa
    14341 attgacgaaa gtgtcaaaaa aatcaatgac attaaagcca aactgttcaa aaacaaaatt
    14401 gatcaaagag tcgaatttct aaaaactctg accgatgagc agagaaaaat ccttaaagaa
    14461 cagccctgcg gtatgcaaaa agtaaaaaag atcaaaaggt tggaagggcg cttcggttgg
    14521 gatgatatgg gaaaactcgg ttcagaaatt gacgagttac tgggcttggt gatggacatt
    14581 gatgttggcg aaatggaaat cgagcctgaa gattaattag atgattgagc aaatcctcaa
    14641 ctggatgaag cacgtagaaa taggagaggg cgatctcact actgagagta tcgcccccta
    14701 tcctttacat tcatccggtc ggttagtgat gtcaagcgac acattattct cagggcgtgc
    14761 tataatggag aaattattag aaagcgccgg ggaacagctc caaatcgaat ggcattttga
    14821 cgagggtgat gaaataccgg ctggtgctac tatttttaaa tttaatggca acggagctga
    14881 aatcctcaaa attcggcggc tgttggaatg gttggctagc agtctctccg ggctggcgac
    14941 cgcaacgcgg gaagtcgtta aattcctcgg cgatcataat aaaaaattag ttgccggaaa
    15001 cagcgtttcc ccgatttttg aagaatttga gaaaatagcc ttcaccaccg gtggtggcga
    15061 aatgccattt agtggcattt ctcagcggat ttacctgact cagaatcatt ttatttatgc
    15121 cggaaaatcc cctgatgaag ttattaccac tctggttgat gaattaggtg ttgcccgcaa
    15181 aaaaataaaa atcgaggtcg aggtcaatga tccgatccag tttgaagaaa tcaataaact
    15241 cgactgcgat gtgatccatt tagtagattt taatgatgag caactgcgca ccgtgttcga
    15301 aaaactcaac ccgcgccaca aaacagttgt tcatctcgca acactcgctg acttccgccc
    15361 ggaatatgat gattatttct ttaaatatgt agccgtagaa gatctgcatc gcacccgcca
    15421 ttactcaccg gcgaggctga ttttttcata aatttaatat cataaaatac atactaactg
    15481 attgaaattc aaagtgttga atttccatta atatttcatt ttatttcccc gggcaagtct
    15541 ctaaaataat ttgagaattt tacaaatagc aatagcgatt acccgaaccg gatcgaatcg
    15601 ttattatact cgcctgttgg cgaagttgct atttttttag aggggaatta ctttctggcg
    15661 actaatttga cgcaggcgcg ctggtgagac gccatagtat ttatggaata ttttatagaa
    15721 acgacttagg cttttgaaac catattccag tgcaatggca aggattttct catcggtttc
    15781 aattatttgt ttggcggcta atttgattct ctgttcatta atgaattcag tcggtgaagt
    15841 gttgagatat tggcgaaagc atttgcatag gtgctgttga gtacaaggag ccagttttaa
    15901 catagttttc agcccaccga tcaggttttc tttccgcttc atttcagaac ataatttatc
    15961 gaaccattgg ggacggctgg gatcgagcgt ttgccattcc agaaaaaagc gcgtgaaaat
    16021 ttcagccaat atcattttga gcttaagacg cgcctgatct aaatcagttt tttggagcgt
    16081 tatgagacgg atcaatcgga gagccaattc ctcagtttca acgagtgaaa gggtaaaggt
    16141 cggtggtaaa accggtccgg tgtaacgtcg caggaagaaa tcattgccta gataagtgct
    16201 gagatcgagg agcagttcga gtttaaatgc gacgttgacc atttcacaga ctgaatcgtt
    16261 tagtttctcg aagctatgaa cgtcgtttgg ccgaatgaaa gtcaacatcc cagcttgcaa
    16321 gtcctgcgtc tcaccgttga caatatgccg gactttgcca ctgataacga tgaaaaattc
    16381 ggcaaactcg tgggaatgaa gcgctgtatt atcgctggca agagagtagt agcgcagatc
    16441 ggatccggca atgatgcttt ctggttgact gctattatcc caggctaaac gatggaaagt
    16501 gatattggcg ccgctctgac tgggaatgaa tttatcttgc gacagaacga tatttttcat
    16561 aatgataata tacagcaata aattgataaa ataaagacaa gttacctcga cgatgtgatt
    16621 ttatgatatg tgaatataaa gtaaatttaa cctatgaata aaatggaaaa caattcaata
    16681 cagggactgc agttggttcg gcaggctttt aatcgccaaa aaaccactcg ggtaccctgg
    16741 gtgccttttg taggctgtca tggcggaaaa ttgatcggtc agaccgcgga aaattacctt
    16801 aagtccggca aactaattgc cgaaggcttg cgaaaagcca atgaacttta tcgtccggat
    16861 ggactgccgg taattttcga cctgcaattg gaagccgagg tgctgggatg tgaactgctt
    16921 tgggcggacg atgctccgcc gtcggtcatt acgcatcccc tcgattcgct taatggcaaa
    16981 aatctatcag atttaccaga atttagtctg gctaaaggcc gctatccaga ggttgcccag
    17041 gctatggaca ttattcgtca agattttgcc aaggacctgg caatttacgg tttgatttgt
    17101 gggcctttta ctctggcgct tcacctgatg ggtaatgaaa tttttctcga aatgtatgat
    17161 catcctgaaa aagttgagga gttattggct tattgtaaag aaattgcttt tcaagtcagt
    17221 gatttttatc tgaaaaacgg tgccgatatt attgcggttg ttgacccaat gaccagtcag
    17281 atttcaccgg agcattttca gcgatttata acaccggcac tcaatccgat tttnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctggaag ccattcgtaa tatagatgaa
    17461 ggcgggcagg taggtttcat tctggcgccc ggctgtgata taccttataa tgttccgccg
    17521 cagaatttac aagccgttgc tgaaatggtg tatgatgatt atcagcggga ggtcgctcgc
    17581 agaacattga ccgctcgtga atttgaagta gattggatag ttttaccgga ttacgccaat
    17641 cgggaggaag tgattcttga cattattacg ctggattcgg ctacctgtgc tccctgcaaa
    17701 tatatggtcg aagccgcgct caaagccacc gctgatttaa gtataaagac aacagttcgc
    17761 gagcacaaaa taactaccag tgagggaatt ggtatgatga aaaagctggg agtgagaagt
    17821 ttaccaacca tttgtattga cggcgaggtg aaattcagtt ccatcattcc cgaccagcaa
    17881 accctaaaag ccgctattct cgaccgctgg aaattcaaaa ctgggcaaga taaatgattc
    17941 caatcagatt agtgacagga tttttgggta gtggcaagac gactctattg aaaaatctta
    18001 tccgtcagaa tcagtcgcga cgtctggttt ttttggttaa tgaattttcg ccgactgata
    18061 ttgacggctc actcattgcc aatttaacac ctgattatct tagcatcccg ggcggtagta
    18121 ttttttgccg ttgtttagcg ggaaaattta tcgaatattt acaggaaatc ccccagcgct
    18181 ttactcagga tggcaatgaa attgacgagc tagtaatcga agccagcggc gtagccgatc
    18241 cgcgagtgat atttaaaatg ttaagcgaaa ccggactaga taaggtttat tatattcggg
    18301 aagtcttaaa tatcgttgac ccagctagtc tgggcaaatt attccacacc ttgacgagca
    18361 ttcgggcgca gatcgaggcg gctgacatta tcttgattaa caaagttgat attgcttcac
    18421 cggaggcaat agctcagact gaaagtctaa tccggcaagt caatccttct gcctatgtat
    18481 atcggacaac tttcagccaa ctgccgaagg atttaccgga aaatccccag cgtttacagg
    18541 tgccagaagg tgaattcgcc ccatgccgcg acccacgata tgctacgata attctgaatt
    18601 ccggcgatat gaactatgaa caactacgtc aggcaattac ccccgtagct gatgatgttt
    18661 accgattgaa aggttatctc tttgacagtc atcaatatct atatgtggat tattcgccag
    18721 ctggctggca aattgaaccg gcgtcctcaa ttgagcaaga accgcaaatt gcaatcatta
    18781 taaatggcgt taatcaagcc aaagtgatcg aacattttaa attatgtggt ttgttagaaa
    18841 ataatgtata tttcggagca aaaaatggca cgtaatgaag aactttatct ggcaattttg
    18901 aaaggcgatc gcgccaaagt gcaggcgatc ttgcaaaatg ccatcaatct taatcaagat
    18961 gtcaatgaat tgctggaaga aactatgatt ccagctatgc gtgaagtcgg cgaacgtttt
    19021 gcgcggaacg aaatttacgt tccggaaatg ctgatttcgg cacgcgcgat gcaagccggg
    19081 ttgaccttaa tcgaaccgat tctggctaag tccaatcaca aagcgcgcgg taaggttgcc
    19141 atcggcacgg taaaaggcga cctgcacgac atcggtaaaa atttggttag cattatgcta
    19201 aaaggcgccg gttatgaggt gcttgacctc ggggtgaatt gcagtgtaga aaagtacgcc
    19261 gaagcagtta acgccgggat caatattctt ttatgtagtg cgcttttgac taccactatg
    19321 atgtatatga aggagattgt tgactatttt aaaagtaata atgccgtaaa aatcgtcgtg
    19381 ggaggtgcgc cggttaccca gagtttcgcc gatatgattg gcgcgcacgc ctacggccag
    19441 aatgccaatg atgccgttcg aattgtagcg aatctaaccg gagcggtcgt ctgaacgtcg
    19501 ctctattaag gaaaaaacct gatgcatacc agacacttat ttttattgat tggacaatct
    19561 aacatggcgg gacggggcga aatggatcgc gttctgccga taagtgatga ccgaatccag
    19621 atgtttagga ataatcgctg gcaaaaagca gttgagccat tgcatgatga tcgggcagac
    19681 ctggcgggtg tagggctcgg tatgagtttt gcctcctcca ttctgaaaag aaatttagtg
    19741 caaaatgtcg gacttctgcc ctgtgcagtc ggttctacgg cgattgaaga ttggttgccg
    19801 ggcacgcccc actatcagcg agcggttgca gtaataaaaa ccgcattagc cacggatact
    19861 tacgaaaata tgctcggcgc tattctttgg catcagggcg aagatgacgc caaagataaa
    19921 attttggctg aaaaatatcg cgaccgaata gtttccctcg tgtggtcttt ccggcgtgat
    19981 ttaaatatgc ccagcttacc tttcatcagc ggtgaactgg gtgatttcat caatgccgca
    20041 actggttacc cctacggacc ggatatttcg gcagttctaa aggaactgga aagcattttg
    20101 ccggattatg cctgcgtctc ggctgaggga ttggcgtcca aagacgacgg cttgcatttt
    20161 aatgcacctt ctttacgaga attcggcaaa cgttacgcgc tggcttatca gcgattactt
    20221 tcatatcggc tgaattaaaa ctaattattt aaaatcttaa gcagtttaaa gcgactcaag
    20281 cattgagccg gttattaact caccgctatg cacaatccgc gaattatggc aattgacccg
    20341 cgtgcagatt ccaacacaat cgttaaaatt acattctcga gtaagaaaaa aagccgcaag
    20401 attcccgccg cactcacatt gcgcaatgat tggcatttcc acatcgcaag tcgggcagat
    20461 caatttcagt aatttgccgt caatcaggtc tatatcaagt gaaaaacgcg aatgctcacc
    20521 ataaaatggg ctgagggcaa tcagtccgat cgcttcatcc tgacgaactt ttattaaaat
    20581 acctgggtag ccattaaaag tgactctttg gttgatgagg ttatgaccgt tcggacaaaa
    20641 taattgatta acgaccagta attttggttt ttcggtaaat ttcccacgca tcgggttggg
    20701 aataattaac atacctttat tgtcgaagat tttcattttg aactcctatc agacaattat
    20761 aaaagtaatg ttagcaattt tataaatcaa caggtctttt tgaacttaaa gtaattgtta
    20821 tattcccaaa tcgtacaaac tactaacgaa aaatagtttt tggaattgta tttgagttcc
    20881 taaattaagc cg
//
LOCUS       DAOMJU010000032        20891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2376_length_20891_cov_24.541851, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000032 DAOMJU010000000
VERSION     DAOMJU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 20891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20891
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2376_length_20891_cov_24.541851"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..234
                     /locus_tag="PLO58_04685"
     tRNA            162..234
                     /locus_tag="PLO58_04685"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:195..197,aa:Phe,seq:gaa)
     gene            938..2686
                     /locus_tag="PLO58_04690"
     CDS             938..2686
                     /locus_tag="PLO58_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HQO74119.1"
                     /translation="MKGYFSLVLHSHLPYVINHGEWPHGMDWLYEAAAETYLPLLREF
                     NSLIADGISPNITIGITPILAEQLAAERFKDGFINYLEIKIKAARDNRDEFATVGNLH
                     LEKLAGMWENYYQGLIDEFNSLPDRNIIQAFKKLQDGNHIEIITCAATHGYLPLLGND
                     EAIDAQVRIGKETYRRHFGCDPRGMWLPECAYRPSYQWSSPIKEYPKTFMRRGIEEFL
                     FKYDIQYFIVDKHLILGGKNRGVYIDRYESLKQLWQQFEKNWTPPPEEKSRSIYQPYL
                     VSSTGTGRVAVAYGRHEESALQVWSGEWGYPGDGRYLEFHKKHFPGGHRYWRVTSAKA
                     DLADKQEYVPENVEEALENQSDHFVEVLRNYILKYYAESGKIGVVTAPFDTELFGHWW
                     FEGPRWLGKVLRKINANPDIQAISLGNHLAKNRPTQVITMPEGSWGQGGFHYIWLNQW
                     TEWTWKHIYAAEDKMVELAEKYRETGDVNLRRILNDLARELLLLESSDWQFLISTWSA
                     RDYAENRVGVHRDQFQNIVRILNDYENNGRLSETHQKYLERMERIDDIFPGLDFTYWS
                     KLPPETGRVDSNNSSK"
     gene            2683..4140
                     /gene="glgA"
                     /locus_tag="PLO58_04695"
     CDS             2683..4140
                     /gene="glgA"
                     /locus_tag="PLO58_04695"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HQO74120.1"
                     /translation="MNIIFIAAEASPFAKVGGLGDVIGALPPVLANRGLSVKVIIPGY
                     RSTWNFKTEPLTQLSNLTVTLGNRVVESQVRRWQNPAQIRHEVYFIDNPEYFGQRNVY
                     CAEDGKPYPDNPERFVFFQKAALKLIAETRWSAEIIHCHDNHTALIPVYLKNNLAKVT
                     PFNSLKSVLTLHNIAYQGVCEMRLKEICDLPEELFKPFGALEWYNSLNPLKGGIIFAD
                     AVTTVSPTHAREIMTDEKLSAGMSVVLANRPTPVVGILNGIDTAEWNPATDVHLFAPY
                     SISNLAGKKINKMKLIETLRLNPALKSRPLLGMVSRLVEQKGVDLLIDSLEKILSLGV
                     GFIILGSGEQHYQFELERLANQFRDQMAVKFDYDNILSHRIIAGSDLFLMPSRFEPCG
                     ITQMHALNYGTVPVVHHTGGLVDTVIPWNGKDGNGFAFCEYRSEAMLKTVLEALQVFE
                     KPDEWQKIIINGMSADYSWEKAATRYIELYENLAE"
     gene            4324..5475
                     /locus_tag="PLO58_04700"
     CDS             4324..5475
                     /locus_tag="PLO58_04700"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQO74121.1"
                     /translation="MMKFGGSSVADTTRLRQVAEIVVRRQQADNRVVVVVSAMGKTTD
                     ALIAQAREVSANPQQRELDMLVTAGERISMALLSMAIQELGGDAISFTGSQSGIITEP
                     THQGARILEVRPFRLIEALEKGYIVIVAGYQGVSTTKEITTLGRGGSDTTAVALAAAL
                     GADACEIYSDVDGVYTGDPHICPQAQLLREIPYEFMQTMALAGAKVLNADAVEFAKKA
                     GITILARKTGDSSGRETKVHNTAMGSGATAIVLAEKVSQINGNGHLPELLTEINKAGG
                     RVMALYQRETFDLLVDRTGIPGGDAGRVAQLAKAAGFSANEVAVVTVVGSGLGARPSL
                     FEKVKVTLSEINITVLGVFTNDLALSIVIEPTYATGAVRALHQALIVGK"
     gene            5673..7358
                     /locus_tag="PLO58_04705"
     CDS             5673..7358
                     /locus_tag="PLO58_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQO74122.1"
                     /translation="MKIYLIQPPIEDFYTTPIRNIPQGLLALAANLPKYEINLLDLRS
                     RVRREIVPPPELASVRQFYRPDDASPFSLYKRYYRFGLSEMEIRRTLPRDGDYYLISS
                     LFITYAETVFELIRLIREIVPKSKIIVGGAAATIQPREFLEQGADFVIKGEGELALPR
                     LLNELEKDRPSLAPIPNLVWRNETGVIQENPVNFIEDLNSLPSADYQTPGTPTYYYQK
                     KPHAILITSRGCPHHCSFCCIHQTMGSRYRLRSVENVLNEMAEKIAQGFRSFDFEDDH
                     LGGERDWFLALLKGIAERFTGLNLSLQAMNGITATNLDDEILTAMWRAGFRSLNLALV
                     TPSEAHQRQVGRPFGTEQYLKVVECANKLGFFITTYLIIGLPGDRAEELLESILFLAP
                     LPVLIGPALFYLVPGTASFQQFSRLGLIPSSVLCYRSSFYPVDSKECSRVAAMTLFRI
                     CRILNFMREVIDNEKTPHCPEIESDRIFLEKDLNGRESRYSLGLGLLKLLFERGILYG
                     TGRKEGNFYPIIREICDQKMIERFLSSTWQISGLHRPTVFLKDEFVRRFLLDQ"
     gene            complement(7415..>9088)
                     /locus_tag="PLO58_04710"
     CDS             complement(7415..>9088)
                     /locus_tag="PLO58_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQO74123.1"
                     /translation="MYSKSEIIEMYLNSNWMGHGVYGIQAAAQRFFDKNSEQLTPDEC
                     ALLAGIIKNSVRFSPIRNPINAYQRRNLVLHRMHKYGYLSDEEYALYKNTPLQVFKPE
                     LPPERAPYFVEYIRQWLQREDEKLGIDIYRDGLSIYTTIDSKIQAAADSALTRHLQYQ
                     QNLLNSRLLSNPPELRDFIKDTTISLAQVRDMIRGNIPMSPSMQASLVVQGALVAIDP
                     ATGHILAMIGGRDFRESEFNRATQAKRQPGSAFKPIVFAAAIDNGFPVTTQLLNQPVV
                     LNMPDGSRWAPKNYDLSTGGPTTLREGLAKSLNLISVRVIQELISPTSVVELAKRMHL
                     TTNILPVDALALGACEVIPIELTSAFGIFENHGVWVEPIAITRIDDRYGNTIAQYKPR
                     QELVLSEETAYLVTSLLESAINVGTGAGARSVYGFRLPAAGKTGTTNNFNDAWFIGYT
                     PYMVAGVWVGVDNPAISLGNRQSGAAAALPIWANFMRESHNTKGWKAKNFERPDGIVE
                     VQICSETKLLPSTYCPIETEVFTKQTVPTEHCQIHNEVGKPRPKDNVVF"
     assembly_gap    9089..9188
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9320..10138)
                     /locus_tag="PLO58_04715"
     CDS             complement(9320..10138)
                     /locus_tag="PLO58_04715"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQO74124.1"
                     /translation="MKLKAEPEKISEAVEFLQKRLKNQSAKIAIVLGSGLGYFAEQLE
                     IVDRIDQQEIPHYPTSTVVGHAGKFIICNISGIPLIAIQGRTHLYEGRTLAEVTFYVE
                     ILARLGIRYLILTNAAGGIDPKLKPGSIVLLTDYISFTQIPLTPDGQSQTASPFCAEM
                     IAMVREIALQNKIELHEGIYCWTLGPSYETPAEIQAARKLGATVVGMSTIPEAVTAQR
                     LGLKVLGISLVTNLAAGISESPITHEEVQVTANRSKEVYSILMEKIIIELAKND"
     gene            10545..10778
                     /locus_tag="PLO58_04720"
     CDS             10545..10778
                     /locus_tag="PLO58_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HQO74125.1"
                     /translation="MDPFSWSYTFTMYLRAIGWALVAALGFSLGIGLAIKVFNLLSKD
                     INEWEEIKKGNISVALIIVSLILMVGILVYKVI"
     gene            11126..11587
                     /locus_tag="PLO58_04725"
     CDS             11126..11587
                     /locus_tag="PLO58_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HQO74126.1"
                     /translation="MELDKILTPRLIVYPLSAETKEEVISILVERLHDEGLISNPEAA
                     YQAVIEREKLMTTGIGKGVALPHGKYRETMEVLVAAGVSPEGIDFDSIDAQPVHIFIL
                     LLTPERFPSKHLKLLSKLSRMLNNANCRAEILAAKSAKEIADIFYKYDSQV"
     gene            11597..11932
                     /locus_tag="PLO58_04730"
     CDS             11597..11932
                     /locus_tag="PLO58_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74127.1"
                     /translation="MPFCPRCLYEYVEGTSKCIDCNAELVARLPEEKMTDVKWFCLGV
                     LSDIVMAEMVKESLENNGIETILRTDPLHTAFSLQSTGATGSYAKLFVPCGKKKTALD
                     ILKSMSTTG"
     gene            11967..>12275
                     /locus_tag="PLO58_04735"
     CDS             11967..>12275
                     /locus_tag="PLO58_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQO74128.1"
                     /translation="MLYQRVKGTRDILPSEVHQWQFLENFLREYFKRNNYKEIRTPAF
                     EVTELFTRSIGGDTDVVSKEMYTFLDKGGTSITLKPELTAPVMRAYIQNNLEQISPLT
                     K"
     assembly_gap    12276..12375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12376..13104
                     /locus_tag="PLO58_04740"
     CDS             <12376..13104
                     /locus_tag="PLO58_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQO74129.1"
                     /translation="AIEPYHAELCGACQERLQKNVLRLFDCKVEICRQIMAEHAPSIL
                     DYLSNDDRLHFKKVCHLLDVSNVPYTIDQALVRGLDYYTRTTFEITSPLLGAQDALCG
                     GGRYDHLIADLGGKPTPGVGVASGIERLLLVLEQVGSFPEPTDNLVYVITMSENSRTA
                     GFRLVTELRRKKVNAQMDFLRRSIKAQLRDADREQANWVVIIGEDEISRNIVIFKDMK
                     NGDQRELPLNTAADQIVALLQDKT"
     gene            13150..>13928
                     /locus_tag="PLO58_04745"
     CDS             13150..>13928
                     /locus_tag="PLO58_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HQO74130.1"
                     /translation="MSFLNPGFLWLLPLAGIPLLIHLLGRQRFQVVEFSSLRFLKSLQ
                     ADVLRRLKIRQIILLIIRTLLILCLILIFARPYRSSQTPGIYMAKGANLYLIVDNSAS
                     MSLKCDGRTQLSRGIEAILSSAKNIDFPVNLNLILATETAGISNRTTVYSLSDLEREL
                     VHTPETNFAAQLLPAIALAVEEIIKNYDLNGVIWLVSDFQSSNWNRKSPMEQKLLAEI
                     IANKIRLVLFPVTGEADNSALGAIHFNEQIYAKDKPVTLQST"
     assembly_gap    13929..14028
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14029..14595
                     /locus_tag="PLO58_04750"
     CDS             <14029..14595
                     /locus_tag="PLO58_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74131.1"
                     /translation="EAGRAVMMATVPKADWSDLPFSGFFPVILQRMTQYIAGGATQPL
                     NYTNGDTVIITRIPGENSIPQVQTPSGRRFNPLVASNRFSFDNTAEIGIYSVIREGGV
                     IEKFAVNLPLSETAAEFFSVADYKNLIKNAPANITVVSETSTDEISQLTLTREFSTLL
                     LFCALFLAIAETFIGRLNRIEMVNPPAE"
     gene            14592..15905
                     /gene="hflX"
                     /locus_tag="PLO58_04755"
     CDS             14592..15905
                     /gene="hflX"
                     /locus_tag="PLO58_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQO74132.1"
                     /translation="MSKLPDQSKIFPTGAVLPSEKVLLVGIINSNQPRHITEEHLTEL
                     ELLMNTLGYEMADKQFVNQRAVDPAYFIGRGKANEIRDFLKSQNISEIVFDDDLSPTQ
                     MRNLQEFLDVEVRDRTGVILEIFASHAHTNEARTQIELATLEYLLPRLTRRWTHLERQ
                     IGGIGVRAGSGETQIETDRRLIKTRIAKLKSELVAIDRQRTVQSQRRANCFRIALAGY
                     TNAGKSTILNLFTGADVLVEDKLFATLDTTVRQLNLDGYPNVLLSDTIGFIRKLPHNL
                     IASFRSTLREIIDADLIIKVADISSPHCLQQLESVEEVLNELGAGNRPTLIVFNKIDR
                     LDENVFKIAQKTYPEAVFISALNQLRIDDLIQALERSIAREGRYLRIQTPFEEVSDCA
                     LIRNHTTVQQEIYDDKVVIFEVFCYKNVWNWLARQLSSATTVLEM"
     gene            16087..16920
                     /locus_tag="PLO58_04760"
     CDS             16087..16920
                     /locus_tag="PLO58_04760"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HQO74133.1"
                     /translation="MNDFAGKRFNLFGASRVGVSLAYHLNRLGFKPEFVWNRSPERLA
                     QAKQAVPFQNFSTDLTAACRDKPDWIIIAVTDDAIEEIATQISQCLPDFKAVKVFHTS
                     GFLSSMALSSLKRKGAATGSLHPVVSVPDITTGIRLLGQCVYTCEGVIKKSLAEIVRS
                     IGGQPYLLNRKQKETIHLSAVFLNNYVVALINAIKILNSDSNLEPSKAQAILQPITEQ
                     AVLNGWEKNVADALTGPLMRGDYQTIEKHLALLNNYPELKKLYQDFIDLALQVVAHSK
                     R"
     gene            17151..18512
                     /locus_tag="PLO58_04765"
     CDS             17151..18512
                     /locus_tag="PLO58_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQO74134.1"
                     /translation="MDKKENLPIVIYGGPLFDGINTVYEDGAVYISNGIVEKVGNESE
                     VFPQVTNTEQVEIYDTQGKVIFPGLINLHHHFYAALAKGLSPARPVTNFQEHLENLWW
                     RFDRALDPEAVQLAALLSLLDCIKSGVTTIFDHHSSPSHVKGSLDNIAAVVERSGLRA
                     CLCYEISDRNGRKTFHDSLQENLAFIESHRDQRRLRGILGLHANFTLSEKSMVEIAQY
                     YDPEIGIHIHCAEDKIDREYCRELGYDGPVSRLKHFGLLGKKSILAHGVHLDQAELTE
                     VARSGSIIVHNPESNADNSVGRLNLPPADDVSIGLGTDGMTSAMLATCRAGYLIQRQA
                     GVADTLILNYLPQCLFKTNSTFASQLIGFQLGQIVAGAAADITVFDYIPVTPFNSDNI
                     FRHIIYGMPSARADMVMVDGQQIYTDGVFLTLDEELIREAAQKASQRVWKQFAETNVL
                     NFK"
     gene            18555..18815
                     /locus_tag="PLO58_04770"
     CDS             18555..18815
                     /locus_tag="PLO58_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74135.1"
                     /translation="MTKSFMIIVVNLRKKEAVNVQKILTEWGCLIRTRLGLHEGVLDQ
                     CSDVGSIILELVGERDKHIELARKLNLLSGVKAQYIEIPVEM"
     gene            18841..19818
                     /gene="pfkA"
                     /locus_tag="PLO58_04775"
     CDS             18841..19818
                     /gene="pfkA"
                     /locus_tag="PLO58_04775"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQO74136.1"
                     /translation="MGVLKRIAILTSGGDAPGMNACVRAVVRTAIYDGIDIFGVYGGF
                     EGLINGDVIPLNRRSVSNMIQKGGTFLKTSRSEAFRHKSGRKRAAEHLAEWKIDGLIV
                     VGGDGSFRGTHDLYEESGIQVVGIPATIDNDIYGTDITIGYDTAVNTALDSIDKIRDT
                     AASHDRLFIVEVMGRSAGFIALEVGICGGAEEILVPETKVTLENLGNLIKTWAIAGKT
                     SSLIVVAEGDELGNAHEIAAKLYQQYHTICHVCVLGHIQRGGSPTATDRLLASRLGYH
                     AVKALEDGKSDVMVGWINNSVTFIPLPESWEKKKPLDEELIKIYRILST"
     gene            20337..>20891
                     /locus_tag="PLO58_04780"
     CDS             20337..>20891
                     /locus_tag="PLO58_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HQO74137.1"
                     /translation="MNSWRQLAIATYTAPKDSRIYGTYEVDVTSVLQYIKEKREAGIH
                     LTITYFVAAAIARALYEDMPEVNCFIRRGQVVARENADVFLSVNVGGEAMTGLVLRKC
                     QELSATEIGQITQKVVEKKRDGEEEEGAFAAKDKLAKIPWPFRRPVFLFIKWWIFDMG
                     WTFPFLKIKPDPFGSIMLTNIGTFG"
BASE COUNT         5740 a         4207 c         4794 g         5850 t
ORIGIN      
        1 aactactaac gaaaaatagt ttttggaatt gtatttgagt tcctaaatta agccgttttt
       61 taaaaatggc gaggtagctt cccgccgtag cgggacggga caggcagtct tctccttcgc
      121 tacctcactc ttaaagaaga cgattggcac tttgatatta tggcgaggta gctcagtcgg
      181 tagagcaggg gactgaaaat ccctgtgtcg gcggttcgat tccgtccctc gccactaatc
      241 atagtaaaaa gttaaaagta aaaagtaaaa agtaagtcct tattctcgtt atttcgtatt
      301 tttcttgaaa atttctgtgt cggcagtgcc cctgcccgac taatttgcta tggcgagatc
      361 ggcgatccta cccaactgac tgatgtctat cggtcaggcg ggtttacgaa tttcgatttg
      421 tagtgaatcg aggacaaagg tatctacaac aggtaatagt ttttgaacaa aagtcttccg
      481 taaattttta atgaattgat cgagatgttt ccgacgccga ttatgttggg ttcaagcgat
      541 cagtgatgaa aaattggcac gacattcaga ttggcagttt agcgaacagg agatttttct
      601 ggctacctaa attttaaatt cgcttaataa tttcctcgtc ggtctcataa tatactacct
      661 gactcaaagt gtttaatccc ggttcaccta aaacagtacc accggctctc ttaagtggat
      721 ataaatatag ctgtttttag gcttatggaa tggatagcaa gtatttagat aatgcttgga
      781 atgctgagat aactcatagc ggtttggttt atttctctca aactttcgct gaataaaaag
      841 aatctacaaa ctcgattaga ttcttttccc gcagtttgaa atcttgtggc aatgtcgtat
      901 ttttagtcgt ttgtcaattg ataagtaagg attaaagatg aaaggttatt tttcgttagt
      961 attgcatagt cacctgccct atgttattaa tcacggtgaa tggccgcacg ggatggattg
     1021 gctttatgaa gctgcggctg agacttatct cccgcttctc cgcgaattca atagcctaat
     1081 tgccgatggc attagtccaa acataacgat cgggatcacg ccgattttag ccgagcaatt
     1141 agccgcggaa agattcaagg acggtttcat caattatctg gaaatcaaaa tcaaagccgc
     1201 ccgggataac cgagatgaat ttgcaacagt cggcaatctg catctggaaa aattagctgg
     1261 aatgtgggaa aattattacc agggcttaat tgatgaattt aattccttgc cggatcgcaa
     1321 tattatccag gctttcaaaa aactccagga tggcaatcat atcgaaataa ttacgtgtgc
     1381 cgctacgcat ggctatcttc ctttattagg caatgatgaa gccatagatg ctcaagttcg
     1441 aatcggcaag gaaacttacc gccgccattt cggatgtgat ccgcgcggaa tgtggttacc
     1501 ggaatgtgcc taccggccga gttatcaatg gtcatcacct atcaaggaat atcccaaaac
     1561 cttcatgcgg cgaggtatcg aggaattcct cttcaaatac gacattcagt atttcattgt
     1621 tgataaacat ctaattctgg gcggtaaaaa ccgaggcgtt tacattgatc gttatgaatc
     1681 actgaagcaa ctctggcaac aatttgagaa gaactggacg ccgccgcccg aggaaaagag
     1741 tcgctcgatt taccaacctt atctagtatc ttcgactggg acgggacgag tagcggtggc
     1801 ttatggccga catgaggaat ccgccttgca ggtctggagc ggcgaatggg gctatccggg
     1861 cgacggtcgc tatctggaat ttcataagaa acatttccct ggcggacatc gctattggcg
     1921 agtaaccagc gcaaaggctg atttagccga taaacaagaa tatgtgccag aaaatgtaga
     1981 agaagccctc gaaaaccaat ccgatcattt tgtcgaagta cttagaaact acattttgaa
     2041 atattatgcg gagagcggca aaatcggcgt tgtaacggcg ccattcgata cggagctttt
     2101 cgggcattgg tggttcgaag gcccacgctg gctcggtaaa gtcttgcgta agattaacgc
     2161 caatccggat attcaagcaa tttcactcgg caatcattta gccaaaaatc gtccaactca
     2221 ggtgattacg atgccggaag gtagttgggg acagggtggc ttccactata tctggctaaa
     2281 tcaatggacc gaatggacct ggaaacatat ttatgccgcc gaagataaaa tggtcgaatt
     2341 agccgagaag tatcgcgaaa ccggcgatgt caatctacga cgcattttga atgacttagc
     2401 ccgcgaactg ctattgctgg aaagttccga ctggcaattt ctgatcagca cctggagtgc
     2461 gcgtgattat gcggagaatc gcgtcggcgt gcatcgcgac cagttccaga atatcgttag
     2521 aattttaaat gattatgaaa acaacggccg actgtccgaa acccatcaaa aatatttaga
     2581 acgtatggaa cgcatagatg acattttccc gggactcgat ttcacttact ggagtaaact
     2641 tccgccggaa actggacggg ttgattctaa taatagttca aaatgaatat tatttttatt
     2701 gccgctgagg cttctccatt tgccaaagtt ggtggcttgg gcgatgtaat cggcgcttta
     2761 ccaccggtgc tggcaaatcg tggtctttcg gtcaaagtca taatcccagg ctatcgctcg
     2821 acctggaact ttaaaaccga gccgttaacg caattatcga atcttacagt cacgctgggt
     2881 aaccgcgtgg tggaaagcca ggtcaggcgc tggcaaaatc cagcccagat tcggcacgag
     2941 gtctatttta ttgataatcc ggaatatttt gggcaacgca atgtctattg tgctgaagac
     3001 ggcaagccgt atccggataa tccggaacgt tttgtgtttt tccaaaaagc cgctttaaaa
     3061 ttaattgccg agactcgctg gtcggctgag attattcact gccatgataa tcatacagct
     3121 ttgattccgg tttatcttaa aaataatttg gcgaaagtaa cgccttttaa ttcgctaaaa
     3181 agtgtcctaa ctctccataa tattgcctat caaggtgttt gtgaaatgcg gttaaaggaa
     3241 atctgcgatt tgccggaaga actatttaag ccgtttggag cattagaatg gtataatagc
     3301 ctcaatcccc taaaaggcgg aattattttt gccgacgctg tcacaaccgt cagcccaact
     3361 cacgctcgtg aaattatgac ggatgaaaag ctaagcgccg gaatgagtgt ggttttagcc
     3421 aatcgtccaa ctccggtggt gggaattttg aacggtattg atacggcgga atggaacccg
     3481 gcgacggacg ttcatctttt tgcgccttat tcgattagca atttggctgg gaaaaaaatt
     3541 aataaaatga aattgattga aactttgcgt ttaaatccgg cgctaaaatc acgaccattg
     3601 ttgggaatgg tttcacgttt ggtagaacaa aaaggcgttg atcttttgat tgacagctta
     3661 gaaaaaattc taagtttagg cgtcggcttt atcattcttg gcagtggcga acagcattat
     3721 cagtttgaat tagaacgcct ggcaaatcaa tttcgtgacc agatggcggt gaaattcgac
     3781 tatgataata tcttatcaca ccgaattata gccggaagcg atctttttct gatgccttcc
     3841 cgttttgagc cgtgcggaat aactcaaatg catgccctga actacggcac agtgccggtg
     3901 gtgcatcata ccggcggctt ggtggatacg gtaattccct ggaatggtaa agatggcaat
     3961 ggttttgctt tttgtgaata tcgatcggaa gctatgctta aaaccgtttt ggaagccttg
     4021 caggtttttg aaaaaccaga cgagtggcaa aagataatta taaacggtat gagcgccgat
     4081 tactcgtggg agaaagccgc taccaggtat atagaattgt atgaaaatct ggcggagtaa
     4141 gagtgaaatg acattaaaaa ggatcaggtg ttgaagaaac tgataaatga aaaaaacact
     4201 gagccttttg ctatcgttta aagaatgtta gctactttta gcctgttgtt gaaaaatgca
     4261 attttcagct gaatcataaa ttaaagtgaa aggaagcgcg tcgaaccgat tatgaagacc
     4321 gttgtgatga aatttggcgg cagttcggtg gcggacacta cgcgactgcg gcaggttgcc
     4381 gaaattgtcg tgcggcgtca acaagcagat aatcgggtgg tagtggtcgt cagcgctatg
     4441 ggtaaaacaa cagatgcctt gatcgcccag gcgcgcgaag tctccgccaa tccccaacag
     4501 cgcgaactcg atatgctggt taccgctggt gagcgcatta gtatggctct tttatcaatg
     4561 gcgatccagg aactgggcgg agacgcaatc tctttcactg gaagtcaaag tggcattatc
     4621 accgagccga cccaccaggg cgctcgaatt cttgaagtta gaccgtttcg tctgatcgaa
     4681 gcccttgaaa agggctatat tgtgatcgtc gccggttatc agggtgtatc tacaactaag
     4741 gaaattacga ccctcggtag aggtggttcg gataccacgg cagttgcgtt agccgccgcg
     4801 ctcggtgctg atgcctgcga aatttactcg gacgttgatg gagtttatac aggcgatccc
     4861 catatctgtc cccaggcaca attattgcgc gaaattccct acgagtttat gcaaacaatg
     4921 gcgcttgccg gggcaaaggt gctgaatgcc gacgctgttg aatttgccaa aaaggctgga
     4981 atcacgattc tggctcgtaa aaccggtgac agtagtggtc gtgagacaaa agtgcataat
     5041 actgcgatgg gttcaggtgc tactgccatc gttttagccg agaaagtctc gcagattaat
     5101 ggcaacggac atttgcccga gttactgacg gaaatcaata aagctggtgg ccgggtaatg
     5161 gctctatatc agcgagaaac ttttgatttg ctggttgatc gaactggcat accgggaggc
     5221 gatgccggtc gtgtggctca attggctaaa gccgccggat tctcagctaa tgaagtcgcg
     5281 gtcgttaccg tagtgggcag tggtttagga gcgcgtccct cactttttga aaaggtgaaa
     5341 gtgacattat ccgaaattaa tatcaccgtt ctcggagttt ttacgaacga tttagctctg
     5401 agtattgtaa ttgaaccgac gtatgccacc ggagcagtta gggcattgca tcaagcgctt
     5461 attgtcggta aatgaacgcc ttaattcagt caattttgta aaaatagaaa cgtaaataag
     5521 atcgacattt atcggtcaat gactttttat cattaatcta aatagattgg cgataattac
     5581 tttcttttgg gaattaaggt gcatcaggca gaatatcaaa aaaataattg acagccagaa
     5641 aaaccatttt atcttagatt ataagtggaa atatgaagat ttatttaatt caaccgccta
     5701 ttgaggattt ttatacgacg ccgatacgga acattccgca gggtttatta gcgctggcgg
     5761 cgaatttacc caaatatgaa attaatctgc tggatttgcg ttcaagagtg cgacgagaaa
     5821 tcgtgccgcc gccggaatta gcttcggttc gacaatttta tcggccggat gacgcttcac
     5881 cttttagcct ttataaaagg tattatcgct ttggactgag tgagatggag atcagacgaa
     5941 ctctgccaag agatggtgat tactatctaa tttccagcct ttttataact tacgctgaaa
     6001 cggtttttga attaatccgg ttaatccgtg aaattgtacc gaagtcgaaa atcattgtag
     6061 gcggcgcggc ggcgactatt caaccgcgag agtttttaga acaaggcgct gattttgtca
     6121 taaaaggtga aggcgaattg gctttacccc gtttgttgaa tgaattggaa aaagaccgac
     6181 cgtcattggc gccaattccg aatcttgtct ggcggaatga aacgggtgta atacaagaaa
     6241 atccggtgaa ttttatagaa gacctgaatt ctctaccttc tgccgattat caaacaccgg
     6301 gcactccgac ttattattat caaaagaaac cgcacgctat cctcataacc agccggggtt
     6361 gtccgcatca ttgctctttc tgttgtatac atcagacaat gggttcacgc taccgcttgc
     6421 gctcggttga aaacgttctg aatgaaatgg ctgagaaaat cgctcaagga ttccgttctt
     6481 tcgattttga agatgatcat ctcggcggtg aacgtgactg gtttctggct ttgctgaagg
     6541 gaatagcgga acgatttacc ggtttgaatt tgtcattaca agcgatgaac gggatcaccg
     6601 ctactaatct ggatgatgaa attttgactg ctatgtggcg ggctggtttt cgctcgctta
     6661 atttagctct cgtgactcct tctgaagcgc atcaaaggca agtcggacgc ccctttggta
     6721 ccgagcaata cctcaaggtc gtcgaatgtg ccaataaatt gggatttttc atcacgactt
     6781 atcttattat cggattgccg ggtgatcggg cggaggaatt actggaatct attctttttt
     6841 tagcgccatt gccggttttg attggtcctg ctttgtttta tttagtgcca ggcacggcgt
     6901 cgtttcagca atttagtaga cttggtctaa taccttcgtc agttctatgt tatcgttcat
     6961 ctttctatcc tgttgatagt aaggaatgta gccgggtagc ggctatgact cttttccgaa
     7021 tctgccggat tttgaatttt atgcgtgagg tgatagataa cgaaaaaaca ccgcattgcc
     7081 ctgaaatcga atcagatcgg atttttctgg agaaagattt gaacggtcgt gaaagtcggt
     7141 acagcctcgg attgggtttg cttaaattgc tgttcgagcg tggaattctc tacggtaccg
     7201 gtcgcaaaga aggtaatttt tatccaatta taagagaaat atgtgaccag aaaatgatcg
     7261 aacggtttct aagtagcacc tggcaaatta gtggtcttca tcgtccgacc gttttcctaa
     7321 aagatgaatt tgttagaaga tttcttttag accaataata tagattgcgg aatacaagtt
     7381 tttcgattga gtaattggtt aaatttttgc gcgattagaa aacgacgtta tcttttgggc
     7441 gcggtttacc gacttcattg tgaatttgac aatgttcggt cgggacagtc tgtttagtaa
     7501 aaacttcagt ctcgatcgga caataagttg agggcaggag tttagtttcg gaacagattt
     7561 ggacttcgac gattccgtcc ggacgttcaa aattcttagc tttccagcct tttgtattat
     7621 gagattcccg cataaaatta gcccagatcg gtaaagcggc ggccgcgccg gactggcgat
     7681 tgccgagaga aatagccgga ttgtccactc ctacccagac tccggctacc atatagggag
     7741 tataaccgat gaaccaggcg tcattgaaat tgttggttgt accggtttta ccggcggctg
     7801 gcaatcggaa tccataaaca ctgcgtgccc ctgcgccagt acctacattt atagcggact
     7861 ccagtaaact ggttaccaaa taagccgtct cttcgctcag aactaattcc tgacgcggtt
     7921 tatactgagc aatcgtattg ccataacgat cgtcaatgcg ggtaatagcg attggttcaa
     7981 cccaaactcc gtggttttcg aagattccaa aagccgaggt taattcgatc gggatgactt
     8041 cacaagcgcc cagcgccaga gcgtcaaccg gtaaaatatt agtagtcaag tgcatacgtt
     8101 tcgccaattc gacgactgaa gttggggaaa ttagttcttg aatgacgcgt acggaaatta
     8161 aattgagtga cttagccaat ccttcgcgca aagtggtagg accgccggtt gataaatcgt
     8221 aatttttcgg tgcccagcgg ctaccgtctg gcatattcaa aaccaccggc tgattaagta
     8281 attgagtagt taccggaaaa ccattatcta tcgcggcggc gaagacaatc ggtttgaaag
     8341 ccgagcccgg ttggcgttta gcctgagtag cgcgattgaa ttcggattca cgaaaatctc
     8401 gaccaccgat catagctaaa atatgtccgg tagccggatc aattgccacc agtgctccct
     8461 gaactaccag actggcttgc atactgggac tcatcggaat attaccccgg atcatatcgc
     8521 gcacttgcgc aagggatatg gtagtatctt ttataaaatc acgtaattca ggggggttgc
     8581 tgagtaacct tgaattaagc aaattttgct gatattgtag atgtctggtc agggcggaat
     8641 cagccgccgc ctgtattttt gaatcaattg tggtatagat ggacaaaccg tcgcggtaaa
     8701 tatcaatccc gagtttctca tcctcgcgct gaagccattg ccgaatgtat tcaacgaaat
     8761 acggagcacg ttcaggcggc aattccggtt tgaaaacctg gagaggtgta tttttgtaaa
     8821 gagcatattc ttcatcggaa aggtaaccgt atttgtgcat ccgatgcaga acaaggttac
     8881 ggcgctggta ggcattgatt ggattgcgga tcggcgagaa ccggacggaa ttttttatta
     8941 ttccggctaa taaagcacat tcgtccgggg tgagttgctc ggaattttta tcgaagaagc
     9001 gctgagccgc cgcctgaata ccgtaaacgc cgtgtcccat ccagttggag tttagataca
     9061 tttcgataat ctcactctta gaatacatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnncg agtaaagtaa tgctttcggc cagctaagca gttcagtttc tgtttgttta
     9241 gtgatgcgtc ccatcagttt tatggtattt taaatgaaca aattcaggaa aacaaaaatc
     9301 gagctaaata tagaaaaatt cagtcatttt ttgctaactc aattattatt ttttccatta
     9361 aaatggaata aacttcctta cttctattag ctgtaacctg gacttcttcg tgggtaatcg
     9421 gtgactcact aatgccagcc gccagattag tgactaagct aataccgagc actttcaagc
     9481 caagcctttg ggctgtgacc gcctcgggaa tagtggacat accgacgacg gtagcgccca
     9541 atttgcgagc ggcttggatt tccgctgggg tttcatagct aggtccgaga gtccagcagt
     9601 agatcccctc gtgcaattcg attttatttt ggagagcgat ttctcgtacc atagcgatca
     9661 tttcagcgca aaagggcgaa gctgtttggc tttgaccatc cggcgttagt ggtatttggg
     9721 taaaactaat ataatcagta agtagaacta tactacccgg ttttagtttt ggatctattc
     9781 caccagccgc attggtcaga atcaagtagc gaatgccgag gcgggctaaa atttcgacat
     9841 aaaaggttac ctcagccaga gtgcgtcctt catagagatg agtccgtcct tggatggcga
     9901 tcaacggtat gccggagatg ttacaaatga tgaatttacc ggcgtgaccg acaaccgtag
     9961 aagtcggata atgtggaatt tcttgttgat caattcggtc aacgatttcg agttgctcag
    10021 caaagtaacc caaccccgat ccgagcacga ttgctatttt ggcactctga ttttttaacc
    10081 gtttttggag aaattcaact gcttcagata ttttctccgg ttcagctttt aatttcattt
    10141 ccaaaatgct ctatcgtttt attaaaattg cgtcgcaaat tagacacaat aattcaactt
    10201 ggcaataaaa actagtggtg aaaggccgaa ataactaaac caaattggaa ctagataaat
    10261 tgagcagaag tttgaaattc gatcagaaga atgatccgat tgataataca ttttctaatc
    10321 aaaaaagaac cctcctatat cttaggcttt cttcgattac tggcgactgg aagaaatctc
    10381 ccataatcct gccctgcttt aatttggtct cattggaaaa aagtagttga taaaaaaaat
    10441 tgattattag taacttaatt acggatcgaa tcataagatt ggcaataaat atttgaaaag
    10501 aaaaaactca attagaatca ataaaactca aaaggaggta tcaaatggac ccgttttcct
    10561 ggtcttatac tttcacgatg tatcttaggg ctattggttg ggcactcgta gccgcgctcg
    10621 gtttctcgct cggcatcggt ttagctatca aagtttttaa cctgctgtca aaagatatta
    10681 atgaatggga agaaattaag aagggcaaca ttagcgttgc gttgattatc gtgtccctga
    10741 ttttgatggt cggcattctg gtttacaaag tcatttaagt taaattcttt ttaaaaatgt
    10801 agtaaaaaag ccaatagttc aggctggtgt tttttaaatg actgaagttc tcttctaatt
    10861 atattttgag atattctgcc gcatttgact ggtttgaact gttttaacat ccctcttttg
    10921 aatgttagga aaggtcgggt tatcggtgcc agtgatcggc aatttttgca ctcagcggct
    10981 acgatctaaa tccgatgatt ttgtccattt attttgactg ttttgaaaat tggactatcc
    11041 gttatatttt tggaatgatt aaagttgtgt aaaaatgctt tatactataa atttgttaag
    11101 atttttgaat gagaagtaaa gtctaatgga acttgataaa attttgaccc cgcgcttgat
    11161 cgtctatcca ctttcggcgg aaacgaagga ggaagtaatt agcatattgg tggaacggtt
    11221 acacgatgaa ggcttaattt ccaatccaga agccgcctat caggccgtca ttgagcgcga
    11281 aaaattgatg acaaccggga tcggcaaggg agtcgctttg ccgcatggca aataccgcga
    11341 aacaatggaa gtgctggttg cggctggtgt ttcgccagaa ggcattgatt ttgattcgat
    11401 cgatgcccaa cccgtgcata tttttattct tctcttgact cccgaaagat ttcccagcaa
    11461 acaccttaaa ttactcagca aattgtcgcg tatgctgaat aatgccaatt gtcgagcgga
    11521 aatcttagcc gctaaatcgg caaaagaaat cgccgatatt ttttataaat atgattctca
    11581 agtatagagg ttagaaatgc cgttttgtcc ccgttgttta tatgaatacg tcgagggcac
    11641 ctcaaaatgc attgattgta atgctgaatt ggtcgcccgc cttccagaag agaaaatgac
    11701 tgatgtcaag tggttttgtc tcggtgtctt atcggacatc gtaatggcgg agatggtcaa
    11761 agaatctctc gaaaataatg gtattgaaac tatcctgaga acagacccgc tccatactgc
    11821 tttttctttg caaagcaccg gcgcgaccgg ctcctacgcc aaattgtttg tgccctgtgg
    11881 taagaagaaa actgctctgg atattcttaa atctatgagt accacaggtt gaccagctta
    11941 aagcaaaata tgagttgata atcaatatgt tgtatcaacg tgtcaaaggc acccgtgata
    12001 ttcttccttc tgaagttcat caatggcaat ttctggaaaa tttcctacgt gaatatttta
    12061 aacgcaacaa ttacaaggag attcgtaccc cagctttcga ggtcacagaa ttattcacac
    12121 gcagtattgg cggcgataca gatgtagttt ccaaagaaat gtacactttt ctcgataaag
    12181 gcggcaccag tattaccctg aaacctgaac tcaccgcgcc ggttatgcgc gcctatatcc
    12241 agaataattt agagcagatt agcccgctga ctaaannnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnngctat cgaaccttat catgctgaac tttgtggcgc ttgccaggaa
    12421 cgtctccaga aaaacgtcct gcgtcttttc gattgtaaag tcgaaatctg ccggcaaatt
    12481 atggctgaac atgctccttc gatactcgat tatctgagca atgacgaccg actacatttt
    12541 aaaaaagtat gtcatttact cgacgttagt aatgttccct atacgattga tcaagcgctg
    12601 gtgcgtggtt tggattacta cacgcggacc actttcgaaa ttacctcgcc tctcttaggt
    12661 gctcaagatg ccctttgtgg cggtggtcgc tatgaccacc tgatcgccga tctcggtggc
    12721 aagccaactc cgggcgtcgg agtggcgtcc ggtatagaac gtcttttgct tgttctggaa
    12781 caggtaggga gctttcccga accgacggac aacttggttt acgttattac aatgagtgaa
    12841 aactctcgaa cggccggctt tcgactggtg accgaactac gccgcaaaaa ggtcaatgct
    12901 caaatggatt tcctgcgtcg ttcgatcaaa gctcagttgc gggatgccga tcgcgaacaa
    12961 gctaactggg tagtaattat cggcgaagat gagatttccc gcaatatagt cattttcaaa
    13021 gatatgaaaa atggcgatca gcgcgaattg cctctcaata ctgccgctga ccagatcgtt
    13081 gcccttttgc aagacaaaac ctgaattgaa tttacttcac ggaaataaat tccagccgca
    13141 ggagttgaga tgtccttttt aaatccggga tttctctggc tcttgccgtt ggctggcatt
    13201 cctttgctga ttcatctact tggtcgtcaa cgatttcagg tggtggaatt tagcagtctg
    13261 cgttttttaa aaagtctcca ggctgacgtt ctccgtcgtc tgaaaattcg acaaattatt
    13321 ctgctaatta tccgaactct gctgatttta tgccttattc tgatttttgc cagaccgtat
    13381 cgtagtagtc aaacccctgg aatttatatg gcgaaaggtg ctaatctata ccttatcgtt
    13441 gataattcgg cttcaatgag tttgaagtgt gacgggcgga cgcaattatc cagaggcata
    13501 gaggcaatcc tatcgagcgc taaaaatatt gatttcccgg ttaatttgaa cttgatcctt
    13561 gccacggaaa cagctggaat cagcaaccga actacggttt acagcctctc tgatttggag
    13621 cgcgaattgg ttcatacccc ggagactaat ttcgccgcac agttgcttcc agcaatcgct
    13681 ttagcagtcg aggagataat taagaactat gacctgaacg gtgtcatctg gcttgtgagt
    13741 gacttccagt ctagtaattg gaacagaaaa tcaccaatgg agcaaaaact actggccgaa
    13801 ataatcgcca ataaaatccg gttagtgctt ttcccggtta ctggtgaagc ggacaactcg
    13861 gcgctgggcg cgatccattt taacgaacaa atttatgcca aggacaaacc ggttaccttg
    13921 caaagcacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga agccgggcga
    14041 gcggtaatga tggctaccgt tccaaaggcg gactggagcg acctgccatt ttccggattt
    14101 ttcccggtca ttctgcaacg tatgactcaa tatattgctg gaggtgctac tcagcccttg
    14161 aattacacaa acggagacac cgtcataatt accagaattc ccggtgaaaa ttccatccct
    14221 caggtgcaaa ctccttccgg acgtcggttc aacccgttgg ttgcgtctaa tcgtttttca
    14281 tttgataata cggccgaaat tggaatttat tccgtgattc gggaaggtgg tgtgattgaa
    14341 aaatttgccg tcaatttgcc gctcagtgaa acagcggctg aattttttag cgtagccgat
    14401 tataaaaatt tgattaaaaa tgctccggcg aatattaccg tggttagtga aaccagtact
    14461 gatgaaatca gccaattgac attgacccgc gaatttagca cgttgctact gttttgcgca
    14521 ttgtttttgg ccattgcgga aacgtttatc ggtcgtttaa atcgaatcga aatggtaaat
    14581 ccacctgccg aatgagcaag ttgcccgatc aatctaaaat ttttccaacc ggcgcggttc
    14641 taccatctga aaaagttctc ctggtgggca taattaattc caatcagccg cgccatataa
    14701 ccgaagaaca tttaactgaa ttagaattac taatgaatac tctcggttat gaaatggctg
    14761 ataagcaatt tgtgaatcaa agagccgttg atccagccta tttcatcggc cggggcaaag
    14821 ctaacgagat tcgtgacttt ttaaaatccc aaaatatcag cgaaatcgta tttgatgacg
    14881 atctttcgcc aactcaaatg cgtaatttgc aggaatttct ggatgtcgaa gtgcgcgatc
    14941 gcaccggtgt tattctcgaa atttttgcca gtcatgccca tactaatgaa gctcgcactc
    15001 agattgaatt agccacactt gaatatcttc tgccacgctt gacccggcgc tggacgcacc
    15061 tggaacgcca aatcgggggc attggcgtcc gagccggatc cggcgaaact caaattgaaa
    15121 ccgaccgccg tttaatcaag actcgaatcg caaaactcaa aagcgaactg gttgctattg
    15181 atcgccagcg caccgttcag agccagcgcc gcgcgaactg ttttaggatt gccttagccg
    15241 gttacaccaa tgctggcaaa tcaacgattc ttaatctttt caccggtgct gatgttttag
    15301 tcgaagataa actttttgcc actctcgata caacggttcg ccagctgaat ctcgacggct
    15361 atccaaatgt tttattaagc gatacaatcg gattcattcg caaattacct cacaacctga
    15421 ttgcttcctt tcgcagtact ttgcgggaaa ttattgatgc tgacctgatt atcaaagtcg
    15481 ctgatataag ttcgccgcat tgtcttcagc aattggagtc agtcgaagaa gttttgaacg
    15541 aactcggcgc tggtaaccgc ccaacgttaa ttgttttcaa taaaattgac cgattagatg
    15601 aaaatgtctt taagattgcc caaaaaactt atcctgaagc ggtattcatc tcggcgttga
    15661 accaactacg aatcgacgat ttgatccagg ctttagagcg atctattgcg cgggaaggac
    15721 gctatctgcg aattcagact ccgtttgagg aagtgtcgga ctgcgctcta attcgtaatc
    15781 acacaactgt tcagcaggaa atctatgatg acaaagtggt aatattcgaa gttttttgtt
    15841 ataaaaatgt ctggaactgg cttgccaggc aattgagttc cgcgacaacc gttttagaaa
    15901 tgtgacgatt ttaactggtt agcggacttg ttatttagct agcgactgtt tactttaaac
    15961 caaatgaagc ggtggaatag atgaagggaa atacagtcca aattcgcaag ataatgggag
    16021 cgttttattc aaaaatgccg atgattccaa ttcgaaaagt cggcggatat ttcttggcga
    16081 tccaatatga atgattttgc ggggaaaagg tttaatcttt tcggagccag tcgagttggc
    16141 gtttcgctgg cttatcatct gaatcggctt gggttcaaac cagaatttgt ctggaatcgt
    16201 agtccggaac gtttagctca ggctaagcag gccgtacctt ttcagaattt ttcaaccgat
    16261 ttgacagccg cttgccgtga taagccggac tggattatca tcgctgttac cgacgacgct
    16321 atcgaggaga ttgcgactca aataagtcaa tgccttccgg attttaaggc ggtcaaggtc
    16381 tttcacactt ccggtttttt aagttcaatg gcgttaagca gtctgaaacg caagggcgcc
    16441 gcaactggtt ctttgcaccc ggttgtcagt gtgccagaca taacaacggg cattcgcctg
    16501 cttggtcagt gcgtttatac ttgcgaaggc gtaattaaga aatccttagc agaaatcgtt
    16561 aggtcaatcg gtggccagcc ttacctgctt aatcgtaagc aaaaagaaac catccaccta
    16621 tcagccgttt tcttgaataa ttatgtagtc gctttaatta atgccattaa aatactgaac
    16681 agtgacagta atcttgagcc gtcaaaagct caggcgattc tacaaccgat taccgagcaa
    16741 gctgtcctca atggctggga gaaaaacgtc gcagacgcct taaccgggcc tcttatgcgc
    16801 ggcgactatc aaacgattga aaaacacctc gctttactga acaattaccc ggaactaaag
    16861 aaattatacc aggatttcat tgatctggcg ctccaggtag ttgctcactc gaaacggtaa
    16921 atattttgtt taaattaatt ttaatgggat ttcgaataat cccgattccg gctcttgaag
    16981 agaattaatt ttttgaatgt tcgatagatt ctactatttt atatcgtgat tatattagaa
    17041 agtttaaagt acgaattgaa tttccagcca gtcttaaacg gtaagttagc tttaaatact
    17101 aatggtggca cattattcta atttattgaa cacagttagc agaggcgttt atggataaaa
    17161 aagaaaatct tccaatagtt atttatggtg gaccgctatt tgatggtatc aacacggttt
    17221 atgaagacgg agctgtgtat atttccaatg ggatagtcga gaaagttggc aacgaatctg
    17281 aggtctttcc ccaagttact aataccgaac aggtcgaaat ttacgatact cagggcaaag
    17341 taatttttcc gggattaatt aatctgcatc atcattttta tgcggcgctg gctaaagggc
    17401 tttctccagc aaggccggta acaaattttc aagaacattt ggagaacttg tggtggcgtt
    17461 tcgaccgggc gctcgatccg gaagcagttc aattggcggc gcttttgtcc ctgttggact
    17521 gcattaaatc cggggtaact acgatttttg atcaccactc atcgccgtcg cacgtcaaag
    17581 gcagtctcga taacatcgcc gcagtcgtcg agcggagtgg cttgcgggct tgtctttgct
    17641 atgaaatttc tgaccgcaac ggccggaaaa ctttccatga cagcttacag gaaaaccttg
    17701 cctttatcga atctcatcgc gatcagcgac gattacgagg aattctcggc ttgcatgcca
    17761 attttacttt atcagaaaaa tcaatggttg aaattgctca gtactacgac ccggaaattg
    17821 gtattcatat tcattgcgca gaagataaaa tagatagaga atattgtcgt gaattaggtt
    17881 atgatggacc ggtcagtcgg ctaaaacatt ttggattact gggtaaaaaa tccattctgg
    17941 cgcacggcgt tcatctcgat caagcagaac tgactgaagt tgctcggagt ggtagtatta
    18001 tcgtacataa tcccgaatcg aatgccgata attccgtcgg gcgattgaat ttacccccgg
    18061 cagatgacgt ttcgattgga ctgggtacgg atgggatgac ttccgcgatg ctggcgacct
    18121 gtcgcgccgg ttatttaatc caacgccaag ctggagttgc cgacactcta atcctcaatt
    18181 atttaccgca atgtttattc aaaaccaatt ccacgtttgc cagtcaattg atcggcttcc
    18241 aattaggtca aattgtagca ggtgcggcgg cggatattac ggttttcgat tatataccgg
    18301 ttacgccgtt taactcggac aatattttcc gtcatattat ttatggaatg cccagcgctc
    18361 gcgcggatat ggttatggtg gatggtcaac aaatctacac cgacggagta tttttaactc
    18421 tcgatgaaga attaattcgt gaagccgccc agaaagccag tcaaagagtt tggaaacaat
    18481 tcgctgaaac caatgtgtta aattttaaat gataattagt tcaacaatat attgctaaag
    18541 tatcaggagg atctatgact aaaagtttta tgatcatcgt cgtaaacctg cgtaaaaaag
    18601 aagcggtcaa tgtccagaaa attttgaccg aatggggttg cttgatccgg acccgactcg
    18661 gtctgcacga aggcgtgttg gatcaatgtt cggatgtcgg ttcgattatt ctggagcttg
    18721 tcggcgaaag agataaacac atcgaattag cccgcaaact caacctgcta tctggtgtca
    18781 aagcccagta tatcgagatt cccgttgaga tgtgagtttt aaatttagaa aggtaaatat
    18841 atgggcgttt taaaaagaat cgcgattttg accagtgggg gtgatgcgcc ggggatgaat
    18901 gcctgcgtaa gagccgtggt acgaacagcc atttatgatg gtattgatat tttcggagta
    18961 tatggtggat tcgaaggttt gattaacggc gatgttattc cgttgaaccg acgttcggta
    19021 agcaatatga ttcaaaaagg tggcactttc ctcaaaactt ctcgtagtga ggcatttcgt
    19081 cataaatcgg gtcgcaaacg cgccgctgaa cacttggctg aatggaaaat tgacggtctg
    19141 atagtagtcg gtggagatgg cagtttccga ggcacccacg atctttatga agaaagcgga
    19201 atccaggttg taggtatacc agctacaatt gacaatgata tttatggtac tgatataact
    19261 atcggctacg atacggccgt aaacacggct ctcgattcga ttgataaaat tcgggataca
    19321 gccgcatcgc acgaccgact cttcattgtc gaagttatgg gacggagcgc cggatttatt
    19381 gctttagaag tcgggatttg tggtggagca gaggagattc tagtgccaga aaccaaggtg
    19441 actctggaga atcttggtaa tttgataaaa acctgggcga tcgccgggaa aaccagcagt
    19501 ctaattgtag tagcggaagg tgatgaactc ggtaatgccc atgagattgc cgccaaatta
    19561 taccagcagt atcacacgat ttgtcatgtt tgtgtgctcg gacatattca gcgtggcggt
    19621 agccctaccg caaccgaccg tttattagcc agtcggttag gctatcatgc agttaaggca
    19681 cttgaggatg gcaagtccga tgttatggtc ggctggataa ataactcggt gacatttatt
    19741 cctttaccgg aaagctggga gaagaaaaaa ccactcgatg aagagctaat aaagatttat
    19801 cgcatcctat caacttgatt aggcaaattc caagctttta aacggtatcc tgcttaacgc
    19861 tagcgctatt tattgggaat agacgggcta gcgagtttgg ttattacctc aaaggtattg
    19921 ttttaatatt acgaatagaa ataatctatc accatcgtcc cagttgaggg atttcaccgt
    19981 caaaatgact gaaaaattga cctgaatgtc acaagcgtga tagttcgaac tattcacttt
    20041 aggatcggaa tgaatttaaa gcgttgtaaa tatattggaa tacaatcggc aggcgttgat
    20101 aaaaatatgg taggaattgg attcgaggaa aagttaattg cgcttaattt gagaagtaaa
    20161 tattgaatca actccggaat gaaacatcaa aaatcgagaa gaaacatagc taatctaacc
    20221 agtatctact tattttcggg ctttggaaaa taatctcgtt tttttttagc aatgttttaa
    20281 attagaggta attttggggt gagtgtaaat taaagttgag taaaacaaaa aatctaatga
    20341 atagttggcg tcaattggcg attgctactt atacagcgcc taaggatagc cgtatttatg
    20401 gaacttatga ggtagatgtt acctcggttc tacagtatat taaagagaaa agggaagccg
    20461 gaattcacct gactataacc tattttgtag cggcggcgat tgctcgtgct ctttatgaag
    20521 atatgccgga agttaattgt tttatccgac ggggacaggt agtcgcccgc gagaatgccg
    20581 atgtcttttt atcggtaaat gtcggcgggg aggcgatgac cggtttggta ttgagaaaat
    20641 gccaggaact gtccgctact gaaattggcc agataaccca gaaagttgtt gaaaaaaagc
    20701 gtgatggcga ggaagaagaa ggtgcttttg ccgccaaaga taaattggct aaaattccct
    20761 ggccttttcg gcggcctgta tttctattta taaaatggtg gattttcgat atggggtgga
    20821 catttccatt tttgaaaatc aaaccagatc cgttcgggag tattatgttg actaatatcg
    20881 gaacttttgg t
//
LOCUS       DAOMJU010000033        20605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2422_length_20605_cov_18.045742, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000033 DAOMJU010000000
VERSION     DAOMJU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 20605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20605
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2422_length_20605_cov_18.045742"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1204)
                     /locus_tag="PLO58_04785"
     CDS             complement(212..1204)
                     /locus_tag="PLO58_04785"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQO74138.1"
                     /translation="MKNEFPTLDFENNLWEQGISHIAGVDEAGRGPLAGPVVAAAVVF
                     DKSSEIISGIQDSKQISPRKRERLFDEIMSQAKAIGIGLVESEEIDRINILRATHKAM
                     RMAIGRLKINVQHILVDGKPLPDKIYPQTAIIGGDRKCYSIAAASIIAKVYRDRLMQQ
                     YDSVFPGYGFAEHKGYGTKKHLESIVKLKPCPIHRISFRGVTEYIVDINKSANTRWLG
                     KYGEDLATLYLFRHGYQIIQRNFHLGAYGEIDIIAQKDNNLCFVEVKTQRHTKFGPPE
                     GWVDERKMRQIGRIASGYLSQNPVENVDCRFDVVTVNLNDSEVQIEHLPDAFRL"
     gene            1579..3555
                     /locus_tag="PLO58_04790"
     CDS             1579..3555
                     /locus_tag="PLO58_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HQO74139.1"
                     /translation="MKEFTIRSLIIGLIMAVVLGAANAYLGLKAGMTIAATYPAAVIG
                     MAILRMMKGTILEENFARTVGSIGESVAAGAIFTIPAFYIAGIWTDFATLPNYLIASS
                     IMLVGGVLGVLFVALLRRVMVEDVELPFPESVAAAEIHKAGQKGGTGAKFLFGAMGLG
                     AGIQALISLKVFTDLKDWFITFKKAIIQLGSSGSATGQSGMVLTAPRVSPAYIGVGYI
                     IGPKLASLNFSGGLLAWGLFVPILTYFIAPSILPDTTAVTTDTWMALSSQVWRNLVRP
                     IAVGGMLMSAIYTLFKMRKSLINGLSRSISDVKKAAQGVQVNERTEQDLKFTTVMFGI
                     SLAAVGTFFIYLFFAKNVIAALVATVVMIIAGFFFAAVSGYLVGIIGSSNNPISGLTL
                     STLIVAALLMVALGMKGTPGVAAVLGVAGVVCVSAAVAGEMLQDLKVGHILGGTPWKM
                     QIGDLIGVALSAVVMFIPLIILHQGDINMGGTGLGGTNLAAPQASLMAMLSQGIVSGE
                     MAWPLIIVGILLGLGFILMQVKSPMLVCVGMYLGLETTFAIFVGGLIRGLLDHYVTKR
                     RCNDAQKSRVENTGVLLAAGLIAGEALVGLLVALLAILKVGYDKWLPSIFNFLPFRFE
                     VSLLIFGLIGWILVSIPLKNAGKADEPAPPSAVM"
     gene            3574..3915
                     /locus_tag="PLO58_04795"
     CDS             3574..3915
                     /locus_tag="PLO58_04795"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HQO74140.1"
                     /translation="MKNKKGEVLNLYEIVPRPLVNSECQADGRIVLLKPKFKNPFLIK
                     LLQRNRRDNFYKLKLDEIGSACWIKFDGYKSAGIICEELESQFGAQIQPVAERVGKFL
                     LMLKKANLIAY"
     gene            complement(4234..4437)
                     /gene="rpsU"
                     /locus_tag="PLO58_04800"
     CDS             complement(4234..4437)
                     /gene="rpsU"
                     /locus_tag="PLO58_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQO74141.1"
                     /translation="MVEVTVYDNEPLERALRRFKKKFEKAGILNDVKKRTYYEKPSVE
                     KRMRRAKAIKRLHRTNAQKTAMM"
     gene            complement(4461..5318)
                     /locus_tag="PLO58_04805"
     CDS             complement(4461..5318)
                     /locus_tag="PLO58_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HQO74142.1"
                     /translation="MSKKDKRVGSDSNRALSKYLEEIGKFEPLSPDKEIELAQRLRAG
                     DRDALRELTESNLRFVVSVAKDYQGQGLPLTDLINEGNLGLIKAAERFDETRGFKFIS
                     YAVWWIRQSILQALAEHSRIVRLPLNRVGTISKITKEAEILEQQYERAPMQDEIASGL
                     AMESEEVSDAIRISRKHQSLNDPFRDGESNALIDVIEDDMQLPPDHPLMDESLKQEIR
                     AALNTLKKREREVIKMYFGIDREYALTLNEIGEEFNLTRERVRQIKENAIRRLSHKSR
                     SKNLKAYLG"
     gene            complement(5479..7485)
                     /gene="tkt"
                     /locus_tag="PLO58_04810"
     CDS             complement(5479..7485)
                     /gene="tkt"
                     /locus_tag="PLO58_04810"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQO74143.1"
                     /translation="MARLCANTLRLLAVDGVQKANSGHPGMPMGMADVATILWSKYLE
                     HNPGDPQWVNRDRFILSAGHGSMLLYSLLHLSGYDVTLEDLQQFRQWDSRTPGHPEYK
                     CLPGVETTTGPLGQGFATGVGMAIAAKITASRFNTDQFQIFGTHKIYGIVSDGDLMEG
                     LSHEAASFAGHLKLGNIIYFYDDNRITIEGKTDLTFSESVAKRFEAYGWEVLQADGHD
                     YISIAKALDEALSKPDRPKLIITRTHIGCGSPSKQDTSECHGAPLGEEEVKITKRNLG
                     FDENVSFYVPEEVRTVYAERRAELEKKYAQWQIEYQKWREANPELAKKYDESQNQVLS
                     IQQEEEIIKAIPSDALATRALSGKIMQKIAELLPNFYGGSADLAPSTSTYLKNLGDIA
                     PDNFSGRNFHFGIREHAMGAILNGLALYGGLIPFGATFLVFSDYMRPPIRLAAMMKLR
                     VIYVFTHDSIFVGEDGPTHQPIEQVASLRIIPNMTVIRPADGLEVAQAWISAINNLGG
                     PTALILTRQKVPVLNRLANFDRHLIDRGGYIISPEQNNALDLVLAASGSEVSVAIEAQ
                     KILQAEGRATRVVSMPSIEKFVRQTATYRESVIPKGKVPVVVIEAGRSTGWGDLIRNP
                     LLTITIDRFGASAPAKVLAEKFGFTAQQVVDRIRDWQVLTNIIV"
     gene            complement(7505..>7918)
                     /locus_tag="PLO58_04815"
     CDS             complement(7505..>7918)
                     /locus_tag="PLO58_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HQO74144.1"
                     /translation="LGGGTELTGGHKGYGLSAVVDILCGVLSAGMVGTEIARYSNAPA
                     GVSHFIGAINPDAFVGTDLISEGIDKYIQMLRNADKAAGQERIYVAGEKEFLAEERYR
                     DFVPLQDKVFQTLNDIGTKVGLSITELKKERTKCE"
     assembly_gap    7919..8018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8019..8173)
                     /locus_tag="PLO58_04820"
     CDS             complement(<8019..8173)
                     /locus_tag="PLO58_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HQO74145.1"
                     /translation="MDNFVRQVLIAHGLNAEDARITADVLIRADRRGIGSHGVARLKR
                     YVDGLDSG"
     gene            complement(8225..9088)
                     /locus_tag="PLO58_04825"
     CDS             complement(8225..9088)
                     /locus_tag="PLO58_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HQO74146.1"
                     /translation="MVHKQKIKNIIFEYCGLAVGSAVMAGSLVFFLIPAKIAPGGVSG
                     LSIVLHYLFNLPVGLVMLVFNIPLFFLGLKILGKEFGPRTFFSFVLVSIFTDFYDKVL
                     GVQAATEDPLLASIFGGILLGVGLGVVFRFKGTTGGSDIVGQIIHKYSNVSVGTGILI
                     ADFFVITFAGITFRDVNLALYGFISLYFSSKVLDLILDGMDYARSFYIISEKQDEIIN
                     LITKEMDRGGTELLGKGFYTRKTRNVLYTVVTRREVATLRQEILKIDPKAFVIIANVH
                     EVIGEGFRKRI"
     gene            9257..10444
                     /locus_tag="PLO58_04830"
     CDS             9257..10444
                     /locus_tag="PLO58_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO74147.1"
                     /translation="MKNYLTPINNKENKRTVRIFAWASFLNDMGSDIIYPVWPLFVTE
                     ILKANMAVLGFVDGLGEAVVSLAKALSGYWSDRIRKRKVFIWSGYLMGAASRIGYAIS
                     TVWQHLIPFRILDRAGKIRGAPRDAIIADVSTNDNRGRNFGLMRMADNLGAVVGILIC
                     LALFKKIGYRALFILAAIPSLISVLLILIKIKEPSHTEKVIYKGMTLRDLDRNFVLFM
                     ILSGIFAIGNFTYSFLLIRAKQIGFPEAFVPVLYLVYTLSASLTAYPFGRLADRIGRK
                     TVMQMSFLLWGLVCLDVIFFQSKIMIFLMFVIYGLHKGALEPVQSTIVSELAPEKFRA
                     SSLGTYQMLIGLCAFPASLIAGFLWDKIGALAPFYLSLGLTIVASALMIFVKESKIVT
                     KSN"
     gene            complement(10445..10909)
                     /locus_tag="PLO58_04835"
     CDS             complement(10445..10909)
                     /locus_tag="PLO58_04835"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HQO74148.1"
                     /translation="MEVNDTNEVKPKPKNIALVAHDHKKKDLLEWVKFNEGTLKQHKL
                     HATGTTGKLLEKELNVKIHKLQSGPLGGDLELGAAIAQRKIDVLIFFWDPLEAQPHDP
                     DIKALLRISVVWNIPVACNRATADYIISSPLMNSVYQPTLPNYSEYLNRLKK"
     gene            complement(10948..11820)
                     /locus_tag="PLO58_04840"
     CDS             complement(10948..11820)
                     /locus_tag="PLO58_04840"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HQO74149.1"
                     /translation="MKIGLLLSPKIELNDPIVPAIIKLLIENKQTVIVADWQSNSLVT
                     NLNGVERCNPEQLPEKIDALFSIGGDGTFLSASHLMAKSRKPVLGIHLGGLGFLADVS
                     VDNYRERLIDFLNGKYSVEERQGLTARTYFKDSIQESFAFNDFVIDKGRISTMIKIRT
                     YINEEYLNTYRADGLIIATPTGSTAYSLSAGGPIISPELNVLVICPICPHSLSVRPVV
                     ISADQVVRIDCTDLSDDVSLISDGHNRIALGAAQKIEIHKAEFPLRIVRFSGDSFFHT
                     LRTKLNWGRDVRGN"
     gene            complement(11833..12096)
                     /gene="xseB"
                     /locus_tag="PLO58_04845"
     CDS             complement(11833..12096)
                     /gene="xseB"
                     /locus_tag="PLO58_04845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQO74150.1"
                     /translation="MAKKSFSLEKSLERLEEILQILESGEKDIEESIKLFEEGMRLSS
                     ECQAHLQALEKRVKLLIEKSDGEICEQDFPESGETPETNNLFD"
     gene            complement(12117..13325)
                     /gene="xseA"
                     /locus_tag="PLO58_04850"
     CDS             complement(12117..13325)
                     /gene="xseA"
                     /locus_tag="PLO58_04850"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQO74151.1"
                     /translation="MAERIYTVTEIARDIKRVVEQFIAPVWVEGEVSNFTISRAGHIY
                     FSLKDQTAVLNCTIWRSQSLSIPFQIVNGMRLLVFGNITTYAMQSQYQLNVQQVRAAG
                     LGSLYLAFEALKKKLSAEGLFDPERKRPIPLYPTRIGLITSESGAALRDFLQISRRRN
                     PSIAIYIYPALVQGAEAAATIIAGIKLFNRLQNVDFIVITRGGGSLEDLWAFNEEPLV
                     RAVAQSAIPIVSAVGHEVDFTLCDFAADLRAPTPSAAAEMTIPERSHILNFIDQTCNK
                     MAQNIRMRLSNFQKEIDNIARRLFLNRPLELLRQRIQRIDDLEAQIVKAINNQIQSAK
                     IGLDNLQKTLVVLNPRAIMERGFAIVYHWPDKKIIKSISEVAVGEQVSVELKDGEFQA
                     KTEIIQSHKN"
     assembly_gap    13684..13783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13900..14838)
                     /gene="nadE"
                     /locus_tag="PLO58_04855"
     CDS             complement(13900..14838)
                     /gene="nadE"
                     /locus_tag="PLO58_04855"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQO74152.1"
                     /translation="MESKLKYLQNSMAIEPEELASSLVEFIKVYVKKLEREGVIIGLS
                     GGIDSAVIAALCKRAVGAENTLALILPEKDSRKEHQVDAQIFANELGIATKLIDLTPY
                     LEMLEVYKLSPVNKPFLPEKIKGTLIGKAYDYYRRKTGETPFSASLTGLRDKEFDSYL
                     KKGNAYYRVKHRLRMLLLYLYAELENRLVVGSTNKSEYEIGFFVKYGCDVANDILPLL
                     NLYKTQVRELARYLNIPAKIREKAPSPDILPGLTDEKALSISYEKLDLILIAFAKSWE
                     VTEIAGALEISEKQVIEIERLIQKSEHMRKIYAPSI"
     gene            complement(15180..16043)
                     /gene="lepB"
                     /locus_tag="PLO58_04860"
     CDS             complement(15180..16043)
                     /gene="lepB"
                     /locus_tag="PLO58_04860"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQO74153.1"
                     /translation="MKEQKQEKKTSGSKFWQEVKSWGIVLLIALGLKATIIEAYQIPT
                     GSMENTILTGDFILGIKFIYGTRTPDWIGIPWTKTGFHIPWFRLPSFKKPEQGEVVIF
                     KYPVDPSLNYVKRCIAGPGQSVEIHDKVVYVDGKIFPNPKHAKFISQYVAPSGWNDPE
                     IFPDGIGNKDNYGPIYVPAKGDTLRFGQIQTGIIKNAAELKGHTFRIYQGKMYIDGNP
                     AEYYIAEQDHYFMMGDNRDNSWDSRFWGFVPFDNVTGRGLITYMSWDKERPLYQLDKK
                     IRWWRIGRLVR"
     assembly_gap    16131..16230
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16436..17929
                     /locus_tag="PLO58_04865"
     CDS             16436..17929
                     /locus_tag="PLO58_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HQO74154.1"
                     /translation="MVKNVVDILIQNRNNRADWPAVIFNEHTFTYSQIYDRSIYLAEF
                     LQKQKIQAGQTIGLLFQNKPEYLIAYYACLLNGFLPVLLPTYLSTNKLTYNFNKLKIE
                     AVLYDSEFHKVVDEIELKKGSPLIKVPSSTWQDTSIDKTNIPTLSTDSFPGINYTNEP
                     AVVSFTDGNSGFPKAVVHSQAGLLANAQNCVKLFGGMRAVRLLCTVPVFHFISHSFIP
                     HSISLAGGTVILVENSKTEEIIRAINSNKVNTLIGTPVFFKEFINYAKTDELSSLKHC
                     IVSGSCLSQETRQNLIRNLGVFVSELYGTTETQMISVVYDEIDQRECSIGQPFPEIDI
                     RIINSSGAIVTPGSVGELQVRSSALMLNYFDDLDKTHFNQTTWFSTGDIVRETSDGSL
                     IFEGRISDSIHKFGYDINPALIEKVLRQHPAIHEAVACPIKNPNHEDQIKVWVSLRES
                     ATVSIDDLNDFCRKNLPGYLQPDAIEVVTYFERDLSGKILRNLHNKS"
     gene            17957..20035
                     /locus_tag="PLO58_04870"
     CDS             17957..20035
                     /locus_tag="PLO58_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQO74155.1"
                     /translation="MKEYQTEEIRNIVLLGHGSSGKTSLSEAMLYSAGATTRLGRVDE
                     GTTVSDYHDDEIERKFSISSSLLNCEWNKIKFNILDSPGYLDFVGEAKALTRVADLAL
                     ININGQSGVEVGTELVFKFAKEDELPVWFIINMLDKEHADFQKSLNSIRSSFTKKAVQ
                     MQIPVNSGPNFDTIADVITQKVIKYKNDQKGTYTELEPTDEIKERLIALKNELIESVA
                     ESDDVLLEKYLSEGTISEEEFLNGLRNAIIKRNLFPVFVAAGLNNIGVTKIMDHIVNF
                     APTPSAKISVSGSYNGTPITRKISDSEPTSLFIFKTISEMHVGELSIFKVISGSLKSG
                     VDLLNSNNGKIERIGQIYCVNGKEKQEVAHLHAGDIGAVVKLRFSKTADTLCDQRAPI
                     TYPGISFPEPVIQIAIEPKSRGDEEKISAGLQSLTSEDPTMSWHYDPELKQTILSGQG
                     ELHLAIILQRLKQKFGVDVNQLPPRIPYRETIKKRGDSKYRHKKQTGGAGQFAEVWMY
                     VEPGPPGSGVEFTNTLVGQNVDRVFVPSVEKGVRSACEEGILAGYRVTDVKAVFYDGK
                     MHPVDSKDIAFQIAGKGAFKECFMNGNPVLLEPIYNLEVIVPEEYMGDVMGDISVRRG
                     KIQGVDSEGPFQKITAKVPLAELYRYSTALRSMTQGRGMHRQSFSHYEQMPKEIQDKV
                     VAASRKEHEE"
     gene            20041..20526
                     /locus_tag="PLO58_04875"
     CDS             20041..20526
                     /locus_tag="PLO58_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HQO74156.1"
                     /translation="MEFDKLWAPWRIGYITNPKCEGCFLCRYPSESDDKKNLILQRGK
                     SAFVIMNFYPYNNGHLMVAPYQHTNDFAALDDATHLELFHLVEKSVGILRETLKAEGF
                     NIGVNLGTVAGAGLKDHLHIHIVPRWTGDTNFMPVIGHTKVISEGLTETWQKLKSKFD
                     E"
BASE COUNT         5767 a         4418 c         4051 g         6069 t
ORIGIN      
        1 agaaaaagaa acagaacatc cggaattttc gttcggctat aaattgctga aaataaatga
       61 gcacaaaaaa ttataactcc gattaatatt actgttaatg ttacttccat aatttcaccc
      121 cattctttcc attaataaca taacgatctt gatctatacc gtaaatatac gattccaaac
      181 atccccatta caaaaataat aaaggcgcta tttataggcg aaaagcatcc ggtaaatgtt
      241 caatttgaac ctcgctatcg tttagattga cagttacgac atcgaaccga caatcaacat
      301 tttcaactgg attctgggat aaatagccag aggcgatccg tccaatttgg cgcatttttc
      361 gctcatctac ccagccttca ggcgggccaa attttgtatg acgctgtgtt ttaacttcca
      421 caaaacagag attattatcc ttctgtgcga ttatatcaat ttcgccataa gcgccaagat
      481 ggaaattacg ttgaattatt tgatagccgt gccgaaatag ataaagcgtc gctaaatcct
      541 caccatattt cccgagccag cgggtattcg ccgacttgtt tatatccaca atatattctg
      601 taaccccgcg aaaggatata cgatgtattg gacaaggttt caacttcacg atagattcaa
      661 gatgtttctt agttccgtaa cctttatgtt cggcaaaacc ataacccgga aagacagaat
      721 cgtactgttg catcaggcga tcgcggtaaa ctttggcaat aatcgaagcc gccgcaattg
      781 aataacactt acgatcgccg ccgataatcg ccgtctgcgg atatatttta tctggcaaag
      841 gtttgccgtc aaccaaaata tgctgaacgt tgatctttaa gcggccaatc gccatacgca
      901 tagctttgtg cgtcgctctc aggatattta tacgatcaat ctcttccgat tcaactaaac
      961 cgatgccaat cgcctttgct tgactcatta tttcatcgaa caatctttct cgttttcgtg
     1021 gagaaatttg cttggaatct tggattccac taataatttc actgctttta tcaaaaacta
     1081 ctgcggcggc aactaccggt ccagctaatg gtccgcgccc ggcttcatcc acaccggcta
     1141 tatggcttat tccctgctcc cagagattat tttcaaaatc cagagttgga aattcatttt
     1201 tcattgtaat aaaaaattta agatacagta tcggattatc aatgaccgaa ttttttgtga
     1261 atggacatta ataattaagt aggggtaaaa catatttcaa gacattcgaa tttaagtcaa
     1321 gacgttttgc cgtttaactt aaatatcttt aatcgtgcga attatggtat gactcaattg
     1381 aaaacgatgg tcatagatta acaaattcgc ttctataatt aataattatg aatggcattt
     1441 ttaaaacttc cttgattatt actcttgctt tttttacttt tatctaaatt tttctaatat
     1501 gaataataaa cattttaaac acaggaggca caattgactg agcataagtt taagccgttc
     1561 gtaccggcta atacggatat gaaagaattc actatccgct cattgattat cggtttgatt
     1621 atggcagtcg ttttgggcgc ggcgaacgcc tatctcggct taaaagccgg aatgacgatt
     1681 gccgctactt atcccgccgc cgttattggt atggcgattt tgcgtatgat gaaaggcacg
     1741 attctcgagg aaaattttgc tcggacggtc ggctcgatag gtgaatcggt cgccgctggt
     1801 gcaattttca cgattccggc attttatatc gctggaatct ggacggattt tgccacttta
     1861 ccgaattatc tgattgccag ctccattatg ctggtcggcg gtgttctcgg tgtactgttc
     1921 gttgcactcc ttcggcgggt aatggttgaa gatgtggaat tgccatttcc ggaatcggtt
     1981 gccgcggctg aaattcacaa agccggtcag aaaggtggta ctggagctaa gtttctgttt
     2041 ggcgcaatgg gtttaggcgc tgggattcag gctttgattt cattaaaagt cttcactgat
     2101 ctcaaggatt ggttcatcac cttcaaaaaa gccatcattc aacttggtag cagtggttcg
     2161 gcaaccggtc agagcgggat ggttctgacc gcgccgcgcg ttagtccggc ttatatcgga
     2221 gtcggctaca ttattggtcc gaaattagct tcgctcaatt tcagtggcgg tttgctggcg
     2281 tggggattgt ttgtaccgat tttgacttac tttattgcgc cttccatact gccggatacg
     2341 actgcagtta ccactgacac ctggatggca ctttcatctc aggtctggcg taatttggtg
     2401 cgaccaatag ccgtcggtgg tatgttaatg agcgcaatct acaccctttt taaaatgcgt
     2461 aaaagcctaa tcaatggact ttcccgcagt atcagcgatg ttaaaaaagc cgctcagggg
     2521 gttcaagtca acgaacgcac cgagcaggat ttaaaattca cgactgttat gttcggtatt
     2581 tctctggcgg cggtgggaac tttctttatt tatttatttt ttgcgaaaaa tgtcatagcc
     2641 gcattagtgg caacggtcgt aatgatcatc gccggtttct tttttgcggc tgtttccggt
     2701 tatctggttg gtattattgg ttccagtaat aatccaatta gtggactgac actttcaact
     2761 ctcatcgtag ccgctctttt gatggttgct ctcggtatga aaggcacgcc cggagtagcg
     2821 gccgtgcttg gcgttgcagg tgtagtctgt gtttctgctg cagtagctgg tgaaatgtta
     2881 caagacttaa aagttggtca cattctcggc ggaacaccct ggaaaatgca aatcggcgat
     2941 ttaattggtg tagctctttc tgccgtagtt atgttcattc cgcttataat tctccatcag
     3001 ggcgatatca atatgggcgg gactggtctg ggcggtacta atcttgccgc accgcaagcc
     3061 agtttaatgg caatgttatc acaaggtatc gtcagcggtg agatggcatg gccattgata
     3121 atcgtcggaa ttttgttggg actgggattc attttaatgc aggtgaaaag cccgatgttg
     3181 gtctgtgtcg gtatgtattt gggattggaa acgaccttcg ccattttcgt cggcggttta
     3241 ataaggggtt tacttgacca ttatgttacc aaacgtcgct gtaatgatgc ccaaaaatca
     3301 cgcgtggaaa atacgggagt cttgttagcc gctggattga tcgctggtga agctttagtc
     3361 ggtttattag tagcattact cgctattctg aaagtcggat acgacaaatg gttaccgagc
     3421 atttttaatt ttcttccgtt ccgattcgaa gtcagtttgt taattttcgg tttaattggt
     3481 tggattttag tcagcattcc attgaaaaat gcaggcaaag ccgatgagcc ggcgccacca
     3541 tcagccgtta tgtaatcaca aggaagttat ttgatgaaaa ataaaaaggg cgaggtattg
     3601 aacttgtacg agatcgtgcc tcgcccttta gttaatagcg aatgtcaggc tgatggcaga
     3661 atcgtattac tcaaacctaa attcaaaaat ccatttttga ttaagctgct ccagcgtaac
     3721 cgacgcgata atttttataa attgaaactg gatgaaattg gttcggcttg ttggataaaa
     3781 ttcgacggct ataaaagcgc cgggattatc tgtgaagaac ttgaaagtca gttcggtgcg
     3841 caaatccaac cagttgcaga acgagttgga aaatttctcc taatgctaaa aaaagccaat
     3901 ctaatcgcat attgatttct caatatattt tacaatttag cgagaagcga aaagcatata
     3961 cctgtgtatc tcatttaact tcgattgaac tgcctacgat aaatttcctc ggtcagtaat
     4021 agcttaggaa acctaaaata tagatatatt accgacttgg gaagtagtct gtctaattat
     4081 ttataaaata ttctcaagta agagataacc aacaaaattc ggattaatct tgctgactta
     4141 gattactcga ctaccgccaa attccgacga gaaaatgtta aaacttttga agtaataacc
     4201 aaaaaatata ttacgtaata atgcccggta attttacatc attgccgttt tttgggcatt
     4261 ggtacggtgt agacgtttga tggcttttgc ccgacgcatt cttttttcga cactcggttt
     4321 ttcgtagtat gtccgtttct tgacatcatt caaaatacca gctttttcga actttttctt
     4381 aaagcgtctt aaagctcttt ccagtggttc attgtcgtac acggtaactt caaccatgca
     4441 atcagtatcc tttctactgt ttagccgagg taagctttta agttcttact tcgtgattta
     4501 tgacttaaac ggcgaatagc attttccttg atctgacgga ctctctcccg cgtcagattg
     4561 aattcttcgc caatttcatt aagagttaaa gcatattcgc gatctattcc gaaatacatc
     4621 tttatcactt cgcgttcacg ctttttcagc gtgttcaaag ccgctcggat ttcctgtttc
     4681 agcgactcat ccatcaatgg atgatcgggt ggtaattgca tatcgtcttc gataacgtca
     4741 atgagagcgt tggactcgcc atcccggaac ggatcattta gcgactgatg tttacgtgaa
     4801 atgcgaatgg cgtcggagac ttcctcggat tccattgcta aaccggaggc aatttcatcc
     4861 tgcatcggtg ctcgttcata ctgctgttcc agaatttcag cctctttggt gattttgctg
     4921 attgtgccga cgcgattaag cggcaggcgt acgatccgcg agtgttcagc taaagcttgc
     4981 agaattgact gtcgaatcca ccatacggcg taagaaatga acttaaaacc gcgggtttca
     5041 tcgaaacgct cggcggcttt aatcagccct aaattaccct cattgattaa atcggtcaaa
     5101 ggcaaaccct gtccctgata atccttagcc acactgacaa cgaaccgtaa attcgattca
     5161 gtcaattcgc ggagtgcatc ccgatcaccg gcgcgcaaac gttgggcaag ttcaatttcc
     5221 ttatcgggcg acaaaggttc aaatttcccg atttcctcta aatatttgct caatgcgcgg
     5281 ttggaatcac tgccaaccct tttgtctttt ttcgacataa actacgaacc ttcgctttca
     5341 ctataaatta aaagcctgca aaaatactaa aaattttaga ttctaccaat atggagaaga
     5401 taatttttga tgcataaata attattactt taatgcaaaa tctgccaaaa tccaaatatt
     5461 gagatcttca aatccctact atacaataat atttgtcagt acttgccagt ctctaatccg
     5521 atcaacaacc tgctgggcgg taaaaccgaa tttttcggct aacactttgg cgggcgcgga
     5581 agctccaaag cgatctatgg taatggtcag caatggatta cggattaggt caccccaacc
     5641 ggtgcttcgt ccagcttcga taactactac tggcacttta cctttcggaa tgacagattc
     5701 acgataggtt gcggtctgcc gtacaaattt ttcgatagat ggcatcgaaa cgacccgtgt
     5761 cgcacgtcct tcagcctgaa gaattttctg cgcttcgata gcaactgaca cctctgaacc
     5821 gctggctgcc aaaactaagt ctaaagcatt attctgttct ggagaaatta tatagccgcc
     5881 acgatcaatc aaatgacgat caaaattggc taggcgattc aacactggta ctttttggcg
     5941 agtcaaaatc aaggcagtcg gtccccccag attattgata gcagatatcc aggcttgagc
     6001 aacttccagc ccatccgccg gccgaattac cgtcatattc ggaataatgc gcaaagatgc
     6061 tacctgctcg atcggctgat gagtcggtcc atcttctccc acaaaaatac tgtcgtgggt
     6121 aaaaacataa ataactcgca acttcatcat tgccgccaac cgaataggag gacgcatata
     6181 atcggaaaaa accaaaaaag tcgctccaaa aggaatcaaa ccgccataca gtgccaagcc
     6241 atttagaatt gcacccatcg cgtgttcacg gataccaaag tgaaagttgc ggccggaaaa
     6301 attatccgga gcaatatcgc ctaaattttt caaataggta ctggtcgaag gtgctaggtc
     6361 agccgagcca ccataaaaat tcggcaaaag ttcggcaatc ttctgcataa ttttaccgga
     6421 gagagcccgc gttgccaggg catctgacgg aatggctttg ataatttctt cttcttgttg
     6481 aatactgagg acttgattct gcgattcatc atattttttt gccagttctg gattagcctc
     6541 gcgccatttt tgatattcga tctgccactg agcatatttt ttctcaagtt cagcgcgccg
     6601 ttcagcgtaa accgtgcgta cttcctccgg aacgtagaaa gagacatttt catcaaaacc
     6661 aagattgcgt ttggtgattt tgacttcttc ctctcccaac ggagcgccat gacattcgga
     6721 agtatcttgt ttactgggac tgccacaacc aatgtgggtt cgagtaataa tgagttttgg
     6781 gcgatcaggc ttggataaag cttcatctaa agccttagcg atactgatgt aatcgtgacc
     6841 gtcagcttgg agaacttccc agccataggc ttcaaatctt ttggcaaccg attctgaaaa
     6901 agtcaagtcg gttttgcctt caatagttat ccggttatca tcataaaagt atataatatt
     6961 gccaagtttc agatgaccgg cgaatgaagc ggcttcgtgc gaaagcccct ccatcaaatc
     7021 accgtcactg acaattccat agattttatg cgtcccaaaa atttgaaatt gatcagtatt
     7081 aaagcggcta gcggtaattt tggcggcgat tgccattccg acaccggtgg caaaaccctg
     7141 accaagcgga ccggtagtcg tttcgacgcc cggtaaacat ttatactcgg gatgacccgg
     7201 cgtgcgcgaa tcccattgtc gaaattgctg caaatcttcc agagtgacat catacccact
     7261 cagatgtaac aatgagtaca atagcatcga accgtgtccg gctgaaagga taaaccgatc
     7321 gcggttaacc cattgcggat caccgggatt gtgttcaaga tacttgctcc acaaaatagt
     7381 agcgacatct gccattccca tcggcattcc gggatgcccc gagttagctt tttggacacc
     7441 atcaaccgcc aatagacgca atgtattggc acacagacga gccatttcca gattaatgtt
     7501 ttgcttactc acactttgtc ctttcctttt ttagttcagt aattgataaa cctactttag
     7561 ttccaatatc atttaaagtc tgaaaaactt tatcttgtaa tggtacaaaa tcgcgatacc
     7621 tctcttcggc gagaaattcc ttttcaccgg caacataaat ccgttcctgg ccggcggctt
     7681 tgtcggcatt tcttaacatt tggatatatt tatcaattcc ttcgctaatt aaatcagttc
     7741 ccacaaatgc gtccggattt atcgcgccga taaagtgaga taccccagcc ggtgcattgg
     7801 aatagcgggc aatttcggtt ccaaccattc ccgcagataa aactccacac aaaatatcca
     7861 ctaccgcgct cagaccataa cctttatgtc cgccggtcag ttcagtaccg ccacccagnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gctatctaaa ccgtcaacat
     8041 atcttttcaa acgcgccaca ccgtgtgagc cgattccgcg gcgatcggca cgaattaata
     8101 catccgccgt aatacgggca tcttcggcat tcaagccatg ggcaattaaa acctgccgga
     8161 caaaattatc cagttcggtt acatttacaa tgacttcttt tgaaataacg cctcctatat
     8221 caaattaaat tcgtttgcga aaaccctcac caatgacttc atgcacattt gcgataataa
     8281 cgaaagcttt cgggtcaatc ttaagaattt cctgtcgcaa agttgcaacc tcccgacgag
     8341 tcaccaccgt ataaagaaca ttgcgagttt tacgggtata aaaccccttc ccgagaagtt
     8401 cggttccgcc acgatccatc tctttagtaa tcaaattgat gatttcatcc tgtttttcac
     8461 taataatata aaaagagcgg gcgtaatcca taccatccag gattaagtcc agcactttac
     8521 tggaaaaata taaactgatg aaaccataca aagccaggtt gacgtctcgg aaagttatcc
     8581 cggcaaaagt aataacgaaa aaatccgcga tcaatatccc agttccgacc gaaacattac
     8641 tgtatttatg gataatttgc ccgacgatgt ccgagccacc ggttgtccct ttaaagcgaa
     8701 aaacgactcc cagtccaact cccaagagaa taccgccaaa aattgaagcc aataacggat
     8761 cctcagttgc cgcctgtact cccaaaactt tatcataaaa atcggtaaaa atcgaaacca
     8821 acacaaatga gaaaaaagtt cgtggcccaa actccttgcc cagtattttc aatcctaaaa
     8881 aaaacagagg gatattgaaa accaacatca ccagaccaac cggtaaattg aacaagtaat
     8941 gtaaaacaat tgataaaccg ctcactccgc cgggagcaat tttggccgga atcaagaaaa
     9001 aaaccagact gccagccata acagccgaac caaccgccaa cccacaatat tcgaaaatga
     9061 tgttttttat tttttgttta tggaccatat aaaactatat tatattttca attttactac
     9121 ttattacgga aaaaatacga ccatagttta ataaattttt aacaaacttt tataataagc
     9181 tagctaaatt atttacattt attgcctatt caattatttc agaataattt tatagggtta
     9241 caattggaga aaaacagtga agaattacct gactcctatt aataacaaag aaaataaaag
     9301 aactgtcaga atttttgcct gggcttcatt cctgaatgat atgggctcgg atataattta
     9361 tccggtttgg ccattattcg ttactgagat tctgaaggct aatatggcgg tgttgggatt
     9421 tgttgatgga ttgggagaag cggttgtttc tttagccaaa gcattgtccg gctattggtc
     9481 agatcgtatc cgtaaacgaa aagtttttat ctggagtggt tatttgatgg gagccgcatc
     9541 gagaatcggc tatgccattt ccacagtctg gcaacatttg atcccgtttc gaattctgga
     9601 ccgcgctgga aaaatcagag gtgcgccgcg cgatgctatt atcgccgatg tttcaaccaa
     9661 tgacaatcgc ggtagaaatt tcggattgat gcggatggct gataatttag gtgccgtggt
     9721 cggtattttg atttgtttag cattattcaa aaaaatcgga tatcgcgccc ttttcatact
     9781 ggcggcaatt ccgtcgctta tttccgtgtt gttaatttta attaaaatca aagaacccag
     9841 tcatactgag aaagtcattt ataaaggaat gactttgcgc gacttagacc gtaatttcgt
     9901 tttatttatg attttaagcg gaatttttgc catcggtaat ttcacttatt catttttact
     9961 aatacgcgcc aaacagatcg gttttccaga agcgtttgtg ccggtgttat acttggttta
    10021 taccctttcg gcatcactaa cagcttatcc tttcggtcgg ttggcggacc ggattggtcg
    10081 taaaaccgtt atgcaaatgt catttctgct ctggggattg gtctgtcttg acgttatttt
    10141 cttccaatcc aaaattatga tatttttaat gtttgttatt tacggtttgc ataaaggcgc
    10201 actcgagccg gttcagagta caattgtttc tgaattagct ccggaaaagt tccgcgccag
    10261 cagtctgggc acttatcaaa tgttaatcgg attatgcgct tttccggcgt ctctgattgc
    10321 tggattcttg tgggataaaa tcggcgccct cgcaccattt tatctatcgc ttggattgac
    10381 gattgtagcc agcgctttga tgatctttgt caaagagtcg aaaattgtta ctaaatccaa
    10441 ttgattattt tttcaaacga tttaaatatt cggaataatt gggtaaggtc ggctggtaaa
    10501 ccgaattcat tagcggcgac gaaatgatgt aatccgcggt cgcccgattg caggctaccg
    10561 gaatattcca gacaaccgaa attcgcagaa gcgctttaat atccgggtca tggggttgag
    10621 cttccagcgg atcccaaaaa aagatcagta catctatttt gcgttgagcg atcgccgcgc
    10681 ccagttccag atcgccgccg agcggaccgc tctggagttt atggattttg acattcaatt
    10741 ccttttcgag tagttttcca gtggttccag tcgcatgcaa tttatgttgc ttcagcgtac
    10801 cttcgttgaa ttttacccat tctaacaagt ctttcttttt atggtcatgc gcaactagcg
    10861 ctatattctt tggcttaggt tttacttcat tggtatcatt cacttccatt gaatttctcc
    10921 ttatacttta tcttttttaa ttataaacta attccctctg acgtctcttc cccaattcag
    10981 tttggtgcgc aaagtatgaa aaaaggaatc gccgctaaag cgcacaattc gaagcggaaa
    11041 ttcagcttta tgaatctcga ttttttgagc cgcgccgagg gcaattctat tatggccatc
    11101 actaattagc gaaacgtcgt cgcttaaatc ggtacaatca attcgcacca cctgatcggc
    11161 cgagataacc accggccgga ctgatagact atgcggacaa atcggacaaa ttaccagcac
    11221 atttaattcc ggtgaaataa tcggtccacc ggcagacaga gaatatgccg tggaaccggt
    11281 gggagtcgcg attatcagtc catcggcgcg ataggtgttc aaatattctt cgttgatata
    11341 ggtgcgaatc ttaatcatcg tggagatccg ccctttgtca atcacaaagt cgttaaaggc
    11401 aaaactctcc tgtatggaat ctttgaagta agtgcgagcc gtcaatcctt gccgctcttc
    11461 aaccgaatat ttaccattaa gaaaatctat caaacgctcg cgataattat caacagatac
    11521 atcagctaaa aatcccaatc ctcccagatg aattccaagc actggtttac gactttttgc
    11581 cattaaatgc gaagcactta agaaagtacc gtcgcctcca atgctgaaaa gtgcatcaat
    11641 tttctcaggc agttgctccg gattacatct ttcaacacca ttcaaattgg taacaagact
    11701 attactttgc caatcggcga cgattacggt ttgcttattt tcaatcaata atttaataat
    11761 agccggaact attgggtcgt ttagttctat tttcggtgat aaaagtaaac cgattttcat
    11821 tctatatcat ccctaatcga ataagttgtt agtttctgga gtttcgcccg attccggaaa
    11881 atcctgttcg cagatttcgc cgtcgctctt ttcgatcaac agtttcactc gcttttccag
    11941 tgcctgtaaa tgcgcctggc attcgctcga taggcgcatt ccttcttcga acagtttgat
    12001 tgattcttcg atatcttttt cacctgattc taaaatctgt aaaatttcct caagccgctc
    12061 gagacttttt tccaatgaaa aacttttttt cgccatttat ttacctcgtt aattaactaa
    12121 tttttatgtg attgaattat ctccgtttta gcctggaatt caccatcttt cagttccacg
    12181 cttacttgtt cgccgactgc tacttcactg attgatttaa tgattttttt atccggccaa
    12241 tgatacacaa tggcaaaacc acgttccatt atggctcgtg gattcaagac taccaatgtt
    12301 ttttgaagat tatcaagtcc tattttagct gattgtattt gattattaat agccttaaca
    12361 atctgcgctt ccagatcgtc tatccgctga atacgttgac gtaacaattc cagaggacgg
    12421 ttcaagaaaa gacgtctggc aatattatca atctcctttt gaaaattgga tagccgcatt
    12481 ctgatatttt gagccatttt attacaggtc tgatctataa agtttaggat atgacttctt
    12541 tcaggaatcg tcatttcagc cgcggctgaa ggagtcggcg cccgcaaatc cgccgcaaaa
    12601 tcgcagaggg tgaaatcaac ttcatgtccg accgcactga cgatcggaat agccgattga
    12661 gccacagcgc gtaccaacgg ttcttcattg aaagcccaga ggtcttctaa tgaaccgcca
    12721 ccgcgggtga ttacgataaa atctacattt tgtaaacgat tgaaaagttt tataccggca
    12781 attatagttg cggcggcttc cgctccctga accaaagccg gatagatata gattgcgatc
    12841 gagggattcc gtcgcctgct aatctgtaga aaatcacgta aagccgcacc gctttcactg
    12901 gtaattaaac cgattcgggt gggataaagg ggaatggggc gtttgcgttc cggatcgaac
    12961 aagccttccg cgctcaactt tttcttcaaa gcctcaaaag ccaggtataa actgcccaaa
    13021 ccggccgctc gcacctgctg aacattcaac tgatactgcg attgcatcgc gtaggtcgta
    13081 atatttccaa aaacaagcag gcgcattccg ttgacaattt gaaaaggaat agacaatgat
    13141 tgactacgcc agatggtgca gttcagcact gcggtttgat cttttaaaga aaaatagatg
    13201 tgaccggctc gactgatggt gaaattcgat acttcacctt caacccagac cggcgcgata
    13261 aattgctcta cgacccgctt gatatctcgg gcaatttcag tgacggtata tatacgctct
    13321 gccataaaaa aaagagtggt gcggacacga agaccatcac cactctcctt ttgtacaaat
    13381 taactaccta attttcttct tatgacggtc tcttctaagc cgtttcttac gcttatgggt
    13441 agcaattttt cttttttttc tttttcttcc acagggcata acccatttac tcctattatt
    13501 tcactacaaa ctctaatgct ggttatagtt gcgactgatc tcacctttaa acagtttaga
    13561 cggtttaaaa acaggaacta tacgcgcggg caaactgatt tcttcattcg tccctggatt
    13621 acgggctttt ttaggtttgc gaacttttaa actaaaattg ccaaaacctc tgaaatccac
    13681 gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttatcaa atattctcaa
    13801 tgttaaaaaa acttatattt tagattgtaa aaatacgtca ggcattgatg attaacaagc
    13861 cttttttcaa aattgaataa atgggtttcc ctgctaacat tatatagaag gggcgtagat
    13921 cttccgcata tgttccgatt tctgaattag acgttcgatt tctataactt gcttttctga
    13981 aatctcaagt gcaccggcaa tttctgtaac ctcccaactc tttgcgaaag cgatcaaaat
    14041 taaatccaac ttctcatagg agatggagag cgctttttcg tcggtaagtc ccggaagaat
    14101 atcgggcgaa ggggcttttt ccctaatctt tgccggaata ttcaggtatc gagctaattc
    14161 tcttacctga gtcttataca agttcaacag cggcaaaata tcattcgcca catcacagcc
    14221 atatttcaca aaaaagccaa tttcgtattc acttttatta gtcgagccaa ctaccagcct
    14281 attttcaagc tccgcataca aataaagtaa aagcattcgc agtctgtgtt taacccgata
    14341 gtaggcattg ccctttttta agtatgaatc aaattcctta tcccttaacc ccgtgagact
    14401 ggcggagaag ggagtttccc cggtttttct ccgataataa tcgtacgctt ttccaattaa
    14461 agttcccttt attttttccg gtaagaacgg cttgtttaca ggcgataatt tatatacttc
    14521 aagcatctca agatacggcg ttaggtcaat taatttcgtc gcaatgccta attcattggc
    14581 aaaaatttgc gcatctactt gatgctcctt tctggaatct ttttcgggta ggattaaagc
    14641 caacgtattt tccgcaccga cggctctttt acataaagcg gcaatcacgg ctgaatcaat
    14701 gccaccgctc aatccaatta ttactccttc cctttccagc tttttcacat acaccttgat
    14761 aaattctact aaagaactgg ctaattcctc cggttcgatt gccattgaat tttgtaaata
    14821 ttttaatttc gattccacgt tttctctcga atattcagca ggctcagcct tacttatcaa
    14881 aacttaacac agtttagcaa tactttggat aaatttgctg ggattttctc ttttgcttct
    14941 aaagagggaa aatactgcgg caacgccagc tcggactcct ccatctagta aagcaggtaa
    15001 gtaatttttt aatcttttcc agcactaata aacattaaac aatagaaaaa tttattaaca
    15061 atccaaatag gataaattcc agttaaaccg gttaaataac cggaaatgaa atttccgatc
    15121 caaacgtcag taacttcttt taattaaaga tgataatatt gaaataaatt tagaagtttt
    15181 tagcggacga ggcgtccgat tctccaccag cgaattttct tatccagttg atagagcgga
    15241 cgttctttat cccaggacat atacgtaatc aatccccgtc ccgtaacgtt atcgaatggc
    15301 acaaagcccc agaaccggct gtcccagcta ttatcgcggt tatcgcccat cataaagtag
    15361 tgatcctgtt cggcaatgta atactcggct ggattaccgt caatatacat tttcccctga
    15421 taaatgcgga aggtatgacc ttttagctca gccgcatttt tgatgatacc ggtttgaatt
    15481 tggccaaaac gaagcgtgtc gcctttggca ggaacataaa tcggtccata attatcttta
    15541 ttgccgatgc cgtcgggaaa tatctcagga tcattccaac cactgggcgc tacatactgc
    15601 gaaataaact tcgcatgttt aggattcggg aaaattttac catccacata taccactttg
    15661 tcatggattt cgaccgattg gccaggcccg gcgatacagc gcttgacata atttagcgac
    15721 ggatcaactg gatatttgaa aataacgacc tcgccctgtt ccggcttttt aaatgacggc
    15781 aaacgaaacc aagggatatg aaaaccagtt tttgtccagg gaattccgat ccaatccggt
    15841 gtccgagtgc cataaatgaa ttttataccg agaataaaat caccggtcaa gatggtattt
    15901 tccatcgaac cggtcggtat ttgataggct tcgatgatgg tagcttttaa acctaaagct
    15961 atgagcagga caattcccca ggacttgacc tcctgccaga atttgctccc acttgtcttc
    16021 ttttcttgct tttgttcttt cataatttta atggcgtgaa tttacaatct ttaactcgga
    16081 gaaccaatta aagttcttaa aattattcaa aatttctcct cagattagaa nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgatttgac cttaaatcat tttattcctc
    16261 tcaaatttga tagcggatta gcaaataata aaatcctctc taatatcttt taatttatta
    16321 aattagatac gctttccaat tgaacaataa cttccggatt attcaattta cttggaatta
    16381 tttggagaat ttgattaaaa ttgcgcgctt tttattttaa ttggctttta tcgtgatggt
    16441 taaaaacgtc gtcgacatac taatccaaaa tcgaaacaat cgggcggact ggccggctgt
    16501 aatttttaat gaacacacct tcacctattc tcaaatttac gaccgatcta tttacctagc
    16561 cgagttttta caaaaacaga agatacaagc agggcaaaca atcgggttgt tatttcaaaa
    16621 caagccggag tatctgatcg cttattatgc ttgccttttg aacggttttt taccagtact
    16681 tttgccgact tacctctcca caaacaaact cacctataac tttaataaac taaaaattga
    16741 agcggttctt tatgactcgg aatttcataa ggttgtggat gaaatcgaac tgaaaaaagg
    16801 ttcgcctctc ataaaagtac catccagcac ctggcaagat acttcgattg ataagactaa
    16861 tataccgacg ctatcaaccg attcgtttcc aggaattaat tacacaaatg aaccagctgt
    16921 ggtatcgttc accgacggca attccggttt ccctaaagcc gttgtacata gtcaagccgg
    16981 tttacttgcc aatgcccaaa attgcgtgaa attgttcggt ggtatgcgcg ctgtgcgttt
    17041 actttgtacc gtaccggttt ttcattttat ttcccacagt tttatcccgc attctattag
    17101 tttagcgggc ggaactgtaa ttttagttga aaactcaaaa acggaagaaa tcattcgggc
    17161 aataaattca aataaagtca acactttgat tggaactccg gtttttttca aagaattcat
    17221 taactatgcc aaaactgacg aacttagttc tttgaaacat tgtatcgttt ctgggagctg
    17281 tttaagtcag gaaactcgcc agaatttgat aaggaatctc ggcgtcttcg tctcggaact
    17341 ttatggaaca accgaaaccc agatgatctc cgtcgtctat gatgaaattg atcaacgtga
    17401 atgtagcatc ggtcagccgt ttccggagat tgacattcgt atcatcaatt catccggtgc
    17461 gattgttact ccgggttcag tcggtgaatt gcaagttcgc tcgtcagcct tgatgttgaa
    17521 ttattttgat gacttggata aaacccattt taatcaaact acctggttta gtaccgggga
    17581 tattgtgaga gaaaccagcg acggctcgtt gattttcgaa ggaagaattt ctgattctat
    17641 ccataaattc ggttatgaca tcaatcctgc tttgatcgaa aaagttttac gacagcatcc
    17701 ggcaattcac gaagcagtgg cttgccctat taaaaaccct aatcacgaag atcagattaa
    17761 agtgtgggta tctttgcggg aatcagcaac cgttagcatt gatgacttga atgatttctg
    17821 tagaaaaaat ttaccggggt atcttcaacc ggatgcaatc gaagtcgtaa catattttga
    17881 acgagaccta tccggaaaaa ttcttcgtaa tctgcataat aaaagttaaa ttttcgcgat
    17941 aatatggagg ctaattttga aagaatatca aactgaagaa ataagaaata tcgtgctttt
    18001 gggacatggt tcgtctggaa aaaccagtct gagtgaggca atgctttact ctgccggcgc
    18061 cacgaccaga ttaggtcggg ttgatgaagg aacaacagtt tccgattatc atgatgatga
    18121 aatcgaacgt aagttttcga tttcctcttc attactgaat tgcgagtgga ataaaataaa
    18181 attcaacatc ttagattcgc ctggttactt agatttcgtt ggtgaagcca aagctctaac
    18241 gcgagtagct gacctggcac ttatcaatat caatggtcag tcgggagttg aagttggcac
    18301 tgaattggtt tttaaatttg ctaaagagga tgaattaccg gtttggttta ttatcaatat
    18361 gctcgacaaa gaacacgcgg atttccagaa atcgctgaat tcgattcgtt cttcctttac
    18421 caaaaaagcc gtgcaaatgc agattccggt caatagtggt ccgaatttcg atactattgc
    18481 cgacgttata acccaaaaag tcatcaaata taaaaatgac caaaaaggta cttacaccga
    18541 gttagaaccc accgatgaaa tcaaggaaag attaatcgct ctgaaaaatg aattaatcga
    18601 atcagtggct gaatctgatg atgttttact tgagaaatat ttaagcgaag gtactatatc
    18661 cgaagaggaa tttttaaacg gcttacgcaa tgcgattatc aagcgcaatc tgtttccggt
    18721 ttttgtagcc gccggcctca acaatatcgg tgtcacgaaa attatggatc atatcgtcaa
    18781 ttttgctcca acaccttccg ctaaaatttc agtcagcggc agttataatg gcactccgat
    18841 aacccgtaaa attagcgata gtgaaccgac ttctcttttc atttttaaaa ctatcagcga
    18901 aatgcacgtt ggagaattat ccatatttaa ggttatttca ggttcgctaa aaagtggagt
    18961 tgatctgctc aattccaaca atggcaaaat cgagcgcatc ggtcaaattt attgtgtcaa
    19021 cggtaaagaa aagcaagagg tcgctcattt acatgccggc gatatcggag ccgtcgttaa
    19081 acttagattt tcgaaaaccg cggatacctt gtgcgatcag cgcgcaccga tcacttaccc
    19141 gggaatttca tttcccgaac cagtcattca aatcgccata gagccgaaat cgcgtggcga
    19201 cgaagaaaaa atcagcgccg gtttacagtc cttaacgagt gaggatccaa caatgtcctg
    19261 gcattacgat cccgagctta agcagacgat tttgtccggt caaggcgaat tacatttagc
    19321 cattatttta caacgtttaa aacaaaaatt cggagttgat gtaaatcaac tgcctccccg
    19381 catcccctat cgagaaacta taaaaaaacg gggtgactcc aaatatcgtc ataaaaaaca
    19441 aactggcggt gccggacaat ttgccgaggt ttggatgtac gtcgagccag gtccgcccgg
    19501 aagcggcgta gaattcacca atacgcttgt tgggcagaat gttgataggg tgtttgtgcc
    19561 ttcggtcgaa aaaggcgttc gctcagcctg tgaagaagga attttagccg gatatcgtgt
    19621 caccgatgtc aaagccgtat tttacgatgg aaaaatgcat cctgtcgatt ctaaggatat
    19681 tgctttccag atcgctggca aaggcgcttt taaagaatgc tttatgaatg gcaacccggt
    19741 tctgctggaa ccgatttata atctcgaagt cattgtgcca gaagaatata tgggtgacgt
    19801 aatgggtgat atttcagtaa ggcgtggaaa aatccaggga gttgattctg aaggtccatt
    19861 tcaaaaaatt accgccaaag tacctctggc tgaactctat cgctatagca cggctctacg
    19921 ctcgatgaca caaggacgcg gtatgcatcg tcagagtttt tcccattacg aacaaatgcc
    19981 caaagaaatt caggataaag tcgtagcggc ttccagaaaa gaacacgagg agtaaattcg
    20041 gtggaatttg ataaactctg ggcgccctgg cgaattggtt atattaccaa tcccaaatgt
    20101 gaaggatgct ttctttgccg gtatccatcc gaatctgatg ataaaaaaaa tctaatcctt
    20161 cagcgtggca aatcggcatt cgtaataatg aatttttatc cctataataa tggtcatcta
    20221 atggtcgctc cctatcagca tacgaatgat tttgccgctt tagatgatgc tactcacctc
    20281 gaattatttc atttagtcga aaagtcggtt ggtattcttc gtgaaactct caaagcggaa
    20341 ggattcaata tcggtgtaaa tttgggaacg gtcgccggcg ctggtctaaa agatcacctg
    20401 catatacata ttgttccgcg ctggaccggc gatactaact tcatgccggt tatcggacat
    20461 accaaagtta taagtgaagg attaactgaa acctggcaaa aacttaaatc taaattcgat
    20521 gaatgatttt tgatagttga atatcgccgt taaatgattt aaatttgtat tgctttttta
    20581 gattatgaat ctggagtagt cccgc
//
LOCUS       DAOMJU010000034        19712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2575_length_19712_cov_20.342880, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000034 DAOMJU010000000
VERSION     DAOMJU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 19712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19712
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2575_length_19712_cov_20.342880"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            568..2262
                     /gene="ilvD"
                     /locus_tag="PLO58_04880"
     CDS             568..2262
                     /gene="ilvD"
                     /locus_tag="PLO58_04880"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQO74157.1"
                     /translation="MRSNQIKKGIERAPHRSLLRALGLTEKDFAKPFIGIANSYTEII
                     PGHAKLNVVGEIVKDAIIKAGGVPFIFNTIGVCDGIAMGHDGMRYSLQSRELIADSVE
                     TMVKAHCFDGLVCIPNCDKITPGMLMAAVRLDIPTIFVSGGPMKAGRTAGGKALDLIS
                     VFEGIGAYQEGKISESELYEIECCACPGWGSCSGLFTANSMNCLCEALGIALPGNGTI
                     LAEDERRIELYKAAGARIVELVRSNLCPSQIITRASIDNAIVMDMAMGGSSNTVLHLM
                     AVANEAGIDYSINRMDEISRKTPTICKVAPSSPYHIEDVDAAGGMSALLNEIFRENEF
                     VNKDCLTVTGKTIAENVAGKRIIDDSVIRHLENPYSPTGGLAIMTGNLAPEGSVVKYA
                     GVSDKMKIFEGPAVIFESQEEACEGILSGKVKAGDVVVIRYEGPRGGPGMQEMLSPTS
                     YIMGRGLGESVALITDGRFSGGTRGACIGHVAPEAAVGGPIALLKNGDRIRIDIPQKS
                     LEVLLSDSELQSRRAQWQARPPRITHGVMARYAEMVASASQGAVLKYNSRIKGEKL"
     gene            2262..3998
                     /gene="ilvB"
                     /locus_tag="PLO58_04885"
     CDS             2262..3998
                     /gene="ilvB"
                     /locus_tag="PLO58_04885"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HQO74158.1"
                     /translation="MKGAEILIDALINEGVDTIFGYPGGVVIPIFDVLYRTDKIRFIL
                     TRHEQGATHAADGYARATGKVGVCLVTSGPGATNTITGIANARMDSIPIVVISGQVDT
                     ANIGTDAFQETDMIGLTRSITKHNYLVKSVEELPIVLKEAFYIARTGRPGPVAIDIPV
                     NVSNAQLKNYQYPSEVTLPGYKPVTNGNPKQIEKLAQAIKTAKKPLLYTGGGIISANA
                     EPELLALLDKTNIPIVITLMGLGSIPSDYPTFLGMPGMHGTMAANLALNECDLLIGIG
                     VRFDDRITGKREQFARQATVAHIDIDPAEIGKVIKPHIPVVGDAKKILSELVKKVSPR
                     PKDEWNERTLAWKNQYPMTYDQDAEEAILPQYVIDRVSALADPDAILVTDVGQHQMWA
                     AQYYRHRKPRSFISSGGLGTMGFGLPAAMGAAVGRPGHTVITISGDGSIQMNIQELAT
                     CSINKIPVKVIILNNSYLGMVRQWQELFWDEHYSQTCLKRSPDCPPQCTGPRKDCPSV
                     YIPDFVKLAEANRIYGYRATKPEEVDDVLKQGLSQPGPVIMEFVVKSIENVYPMIPSG
                     KTISDVIPGRQS"
     gene            3995..4480
                     /gene="ilvN"
                     /locus_tag="PLO58_04890"
     CDS             3995..4480
                     /gene="ilvN"
                     /locus_tag="PLO58_04890"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQO74159.1"
                     /translation="MIEKHTIIVTVVNRVGILARITGLLAQRGYNIESAIAAPTEEKD
                     IYKIILVVKETSENIEQITKQLNKLIDTIKVTDISHKDNYIVREYIILKVGLTPRTRA
                     EVLDLMRVFGAKPIDLATDHLIMELAGHVRKIDRFIELLKPFGIEEYVRSGEFAMTEY
                     N"
     gene            4498..5499
                     /gene="ilvC"
                     /locus_tag="PLO58_04895"
     CDS             4498..5499
                     /gene="ilvC"
                     /locus_tag="PLO58_04895"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQO74160.1"
                     /translation="MSVKLFYEKDVKSDSLKKKTIAIIGYGSQGHAQAQNLRDSGYKV
                     IIAEMPSSPNYALAVKHGFKPVSAAEASEQADVIQILTPDEVQKRVYEQEIKQNLTKG
                     KALVFSHGFNIHFKQIVPPADVDVYMVAPKGPGHLVRRQYEIGAGVPSLFAVYQDVTG
                     QARNLALEHACGIGAGRAGMIETTFREETETDLFGEQAVLCGGVSELIKAGFQTLVDA
                     GYQPEIAYFECCHELKLIVDLIYEGGLAGMYYSVSDTAEYGGMTRGPYIIDCETKKRM
                     QTLLRAIQTGSFAKDWILENQAGRPTLNAYRQEHQELLLEKVGRELREMMPWLKKKG"
     gene            5503..7083
                     /gene="cimA"
                     /locus_tag="PLO58_04900"
     CDS             5503..7083
                     /gene="cimA"
                     /locus_tag="PLO58_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HQO74161.1"
                     /translation="MNRKIQIYDTTLRDGTQAETISFSADDKLHIAKRLDEFGIDYIE
                     GGWPGSNPKDMEFFKLARETEFRHARLVAFGSTKRAGNAIEDDQNIHNLLKAETLAVA
                     IFGKTWILHVEKALRITPAENLELIRESVAYLKSKGKEVIYDAEHFFDGYKDNADYAI
                     QTLQTAQSAGADLITLCDTNGGTLPSEISRIISEVRQKITAPLGIHTHNDSGLAVANS
                     LAAVEAGCVLVQGTINGYGERTGNADLCSIIPNLQLKAGYQCIPSENMKQLTPLSRYV
                     SEIANLAHPNDLPYTGRSAFAHKGGVHVSAVMKDPRSYEHIEPESVGNTRRVLVSDLS
                     GQSNIIFKAQELNIDLERHQDKIPEIVAKLKELEHLGYQYEAAEGSLELLISKIKSEY
                     HDFFKLKGFRILLEKNEDGEQRSEATIRMEVNNTIEHTAAEGDGPVNALDNALRKALT
                     KFYPEIAEMKLSDYKVRVLNPQQATAAKVRVLIESSSDGETWGTVGVSENIIEASWQA
                     LTESISYYLMKKRQKNSK"
     gene            7096..8640
                     /locus_tag="PLO58_04905"
     CDS             7096..8640
                     /locus_tag="PLO58_04905"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQO74162.1"
                     /translation="MAKRIIIFDTTLRDGEQSPGASLTASEKLIIARQLANLGVDVIE
                     AGFPIASEGDFEAVKQIAQTVEGPIICGLARTNEIDIDRCWEAVKYAAKPRIHTFIAT
                     SNIHLEKKLHKTGAEVIEMAYSAIRHAKQYCADVEFSPEDCARTSLEYMCEIVQAALE
                     AGATTINIPDTVGYAEPEEFAERIRYLIDKVPAIRQAVISVHCHNDLGNAVANSLAAI
                     RAGATQVEGCINGIGERAGNAALEEVVMNLVTRKDYFDAETGINTREIFKTSRLVANL
                     TGLSIPRNKPIVGQNAFSHEAGIHQHGVLSSRQTYEIMTPEDVGWEGENLVIGKHSGK
                     HAVAALLEAEGYRPDPEQLNQITNKVKDLADKQKEVAAEDILAIARDVTMQLPEEEQH
                     IQLEEFTVTTGSGLTPTASVRLVVNGERKIGAATGDGPIDAVSNAIWSVIGPSLQLVE
                     YSLKAITGGTNALADVSIKLADQKGNIFAARAVNEDVIKASAMGIVKGINKALSSKQK
                     IHPHTV"
     gene            8662..9924
                     /gene="leuC"
                     /locus_tag="PLO58_04910"
     CDS             8662..9924
                     /gene="leuC"
                     /locus_tag="PLO58_04910"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQO74163.1"
                     /translation="MGATITEKILATHSGREHVKPGELVDCKIDTVMCHDVTTTPAIE
                     LLEKYAIPKVFDPARIVIMPDHFVPNKDIKSAEMVARIRRWAQAQGITQFYDIGCHGV
                     CHALLPEQGFVTPGKTIVCGDSHTCTHGALGAFATGIGSTDLAATLATGKLWFKVPES
                     MRFVVSGRLADGVYAKDIILKIIGQIGVDGALYRALEFCGEVIDELSVEGRMTICNMA
                     IEAGGKSGIINPDAKTLEYVRARTSDEFTVYRSDSDAEYYRTIEIDVSDLEPLVALPH
                     LPSNVKPVREVSAIPIDQAYLGSCTNGRIEDLRIAAQILKNRTVARGTRMIVVPATTE
                     IWKQANREGLLDIFMSAGATVSTPTCGACLGGYMGILAASERCISSTNRNFVGRMGSP
                     KSEVYLASPATVAASAITGKITDPRSYL"
     gene            9934..10422
                     /locus_tag="PLO58_04915"
     CDS             9934..10422
                     /locus_tag="PLO58_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014684490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQO74164.1"
                     /translation="MQGNAHVYARDHINTDEIIPARYLNIDREAELAEHAMEDIDVNF
                     IKKVKPGDFIVSGDDFGCGSSREHAVWALRGAGIKAVIANSFARIFYRNCINNGFLAI
                     ECPGAVRKISDGDRLEIDLAGGKIVNLTKNQTYQFQPLPDFARKIIEAGGLMEQIKKE
                     LA"
     gene            10423..11526
                     /gene="leuB"
                     /locus_tag="PLO58_04920"
     CDS             10423..11526
                     /gene="leuB"
                     /locus_tag="PLO58_04920"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQO74165.1"
                     /translation="MEVKKIAVLAGDGIGPEIMNEALKVLNAVQQKSAIRFEFREALV
                     GGAAWEKYGQHLPEETLQICRNSDAILFGAVGGPVEEQFSPKWRGVEVNALLGLRKTF
                     DLYANLRPAKLFPALVFASPLRQDIAEKGFDILVVRELTGGIYFGQPKGRRGNGAEEV
                     AFDTMIYSRREIERIAHVAFKAARQRRRHVTSIDKANVLTTMVFWREVVTEVAQQYPD
                     VTWESMYVDNASMQLVKNPAKFDVMLCGNMFGDILSDEAAAIAGSLGMLPSASLAESL
                     FGLYEPGGGSAPDIAGQNSANPIAQILSAAMMLTYSFGMNEAAMAIEQAVSDVLQAGY
                     RTADIWQTGCHKIGTAEMGDRIVTELLKKINPE"
     gene            11592..13856
                     /locus_tag="PLO58_04925"
     CDS             11592..13856
                     /locus_tag="PLO58_04925"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQO74166.1"
                     /translation="MDVDFINNFYREFAERVSRLRSELGRALSFAEKTIYGHLFSEQP
                     QSKLKPRESYVNLRPDRVAMQDATAQMALLQFINAGRRQVAVPTTIHCDHLIVAKDDA
                     VTDLKRARRENREVYEFLQTAAYKFGIGFWPPGEGIIHQIILENYAFPGGLMIGADSH
                     TPNAGGLGMLGIGVGGADAVDVMAGLPWELKMPGLIGVELTGRLNGWASPKDVILKLV
                     GMLGTKGATGMVIEYFGDGAANLSCTGKATICNMGAECGATASIFPYDAKMAEYLTLT
                     GRAEVAKAATADFNSILQADPEVYQTPQKYFDRVIKIDLDNLEPYVNGPFSPDMAVPL
                     SQLRDHIKKSGYPAHLSAGLIGSCTNSSYEDLSRAADVARQANRHKIATRAKLLVNPG
                     SQWVKLVCEKAGFLNEFRTLKAQILANACGPCIGQWERDDCEPGIKNSIITSFNRNFA
                     GRNDGNQQTHTFLASPEIVLALTLAGDLNFNPLTDFLISEDGKRYQLEPPEGEELPAV
                     KIQTSRTPARIIHKPDITIKINPLSQRLRLLEPFPAPTSQSFQNLLLLMKVKGKCTTD
                     HISPAGEWLKYRGNLDRISDNLLLGARNAFSGKLGETLNQLTGEYAKVADVARSYQSH
                     QKLSVIVADENYGEGSSREHAAMEPRYLGVVAVIARSFARIHETNLKKQGVLALTFTQ
                     FDDYERVREDDEFSIQSLEKFAPERPLDLIIQHKNGESETIQLQHSYSQLQIEWFWAG
                     SALNYLKLNNPQKL"
     gene            13874..15124
                     /gene="icd"
                     /locus_tag="PLO58_04930"
     CDS             13874..15124
                     /gene="icd"
                     /locus_tag="PLO58_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009791360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQO74167.1"
                     /translation="MSNEQIVFANGSLQVPNFPLIPFIEGDGIGAEICRPTLRLIDAA
                     VNRAYGANRRIIWREVLAGDKSLNQNGEALPAATLQDLNQYVIGLKGPLRTPVAEGRR
                     SLNVILRQTLDLYACVRPLHWFPGVPTPVRRPELLNVVIFRENTEDLYTGIEFMRGSL
                     EAGLLEDFLIGKLKAPEPRFPGENSLGVKPVSKPGSERLIRAAINFAIKYKLPSVTLV
                     HKGNIMKYTEGGFRNWGYELAEREFAESCITAKSGAPAGSLTSGSGDKVLIKDMITDA
                     FFQDILLNPARYSVIATLNLNGDYISDLAAAMVGGIGLAPGANINFETGRAIFESTHG
                     TAPDIAGHDIANPSSLIMSGVMMLEYMGWSEAANLLQKAMANLFIRQIGTVDIFGNVN
                     GARCVGTTEFVDLLEDEILKINIK"
     gene            15144..16496
                     /locus_tag="PLO58_04935"
     CDS             15144..16496
                     /locus_tag="PLO58_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQO74168.1"
                     /translation="MSNSMLQSISQKMQKASQIDPELYVKYNVKRGLRNEDHTGVLVG
                     LTRIGDVVGYRTENGQVIPIEGQLLYRGYQLDDLVKGFQAENRHGFEEICYLLLAGEL
                     PNRSELQYFSDYLAKQRELPENFTNQMLINLVSGDMMNMLARAVLALYTNDPQPEDRS
                     VENLISQSLSLIAKFPTIVAHSYNAYRHAYQQKTLSIRHPHKDLSIAENFLYMLKGNR
                     YTKLEADMLDLALVTHAEHGGGNNSTFTLRVTSSTGTDTYSAIAAAIGSLKGPYHGGA
                     NRKVMEMMTDIKKSLKNWDDRNAVADYLMKILKKEVGDRVGKIYGFGHAVYTLSDPRV
                     KILKSKAADLATEKNRLEEFHLYNLVEELAPQVFADFKGREKSKIVCANVDFYSGFVY
                     SCIGIPPELYTPIFAVSRLAGWCAHRLEELNPQSRRIIRPAYKNVTPEREYVNIAERR
                     "
     gene            complement(16700..18844)
                     /locus_tag="PLO58_04940"
     CDS             complement(16700..18844)
                     /locus_tag="PLO58_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO74169.1"
                     /translation="MRKKPLFLLSIFLFVTFIENVNAQWERIEGLYGGMILGVTGDNP
                     TMYAVSATRLLSKYYLYQSNDMGITWDNTYLDVTNYYDVAAKDSDIFVAFGYPTNNHT
                     CLIHSSDYGRTWESINGIFDSVYVQRISIKDSMVIVTNPYAGLFFSKDNGANWSRSDI
                     NNLPEGRQIWDIAYSDSAIFLSAENCLYRSTDNCVSWDQIYEGIHVIYCIASNGTNLI
                     LNTGYDFYISSDDGDSWTKLSSPDLGSTGGANKLVYYGSHLYFTNISQLFHSADSGNN
                     WTELKLGIDSSEIYRDIYIDSSGVYVATERGMIYSTIDGVNWSYMDLSGITSRSVEEF
                     EFCGNSIFTFSVPPTPYLGNIYSMSTNSWKPIITDPELSLSTFASFGNNQIIAGCRND
                     TSVLCYNENEDIWNKVGNVKTSMIRSTGTELYAIIDSIPYYNNIYKSTDGGYTWDRLL
                     IDDKFGKFTNIFIDDENIFVTKDIYDLPTLALFRSKDSGNTWEFITADTFQINAVLTI
                     DSVMFMGTESSSYNLNGLLRSLDGGKTWDNIFPDKSISSALKRGHNLIFSSGNEVFRM
                     DYLNRSILNISDSLMGNINQLKINDDYLYAGTIRGLWRRPLSDFIVGTHRDKFSTITD
                     YALHRAYPNPFNPKTTFRFDLPEETHVEIVVYDLMGREVWKSAKTHYSAGTYSVVWNG
                     VNQSGQPVGTGMYIVRLKSLRYSATQKVLLMK"
     gene            complement(19077..>19712)
                     /locus_tag="PLO58_04945"
     CDS             complement(19077..>19712)
                     /locus_tag="PLO58_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74170.1"
                     /translation="YFVGYKGSIVHYDGKNFRKMESGTDSPIVDIWGINKDNIWAIAV
                     TNIIDDNHPFGYEDIWFHFNGESWRRKYTASPGDKFVYSPTKLSGYLSPSVWAYRDTV
                     YIPAFSGLWKESIKTGKGKLIHGYINSDFIMDGRLWKISGTSYNDIICMTIWGELFHY
                     NGNGWAHITLLMEQFPEISLYNLSIRGNQLVLVGSLGSYSSAVIIRGHHIK"
BASE COUNT         5526 a         4248 c         4632 g         5306 t
ORIGIN      
        1 ggaagttcca ttggatacgg tttcgatcac ctgataacct aaattagtga cgatggtgct
       61 gatcctttta gctgtttcag cattactctc ggctaacaat atttttagtt ctttcattgt
      121 ccccctcttg gtttagatga tgcatcactg cattttaggt aactttttaa tttacttttc
      181 aattatcccc acaatcgtat tttacaaaga ttattttaaa ctaccaatgt cacaaattaa
      241 agacggctgg tgatttacca attacctgga ttagcgaatt aatctttata acaaacgaga
      301 ttttgttaag ttagatttca cattttaaaa aatttatagc attgattaaa taaaattgtt
      361 gacttacaag ctagaaatat tttatatttg ggagtattaa ttatgagtta cttaaaatct
      421 atacattgca tcgtactacc gtggagcatt attatcctag ctagaggtga aggggtaatt
      481 tagacgtaga aaaaaaataa acagtttcta aaaccccttc taaatcgaga aggggttttt
      541 ttataacaaa ataaaaggaa tatctaaatg cgcagtaatc agataaaaaa aggaatcgaa
      601 cgagcacctc atcgctcact cttgcgggcg ctcggcttaa cggaaaagga ttttgccaaa
      661 ccgtttatcg ggattgccaa ctcttatacc gaaatcattc ccgggcatgc caaactaaat
      721 gtcgttggtg agatagttaa agatgccata ataaaagcgg gtggtgtgcc attcattttt
      781 aatacgattg gcgtttgcga cgggattgcg atgggacatg atggtatgcg ctacagtctg
      841 cagagccgcg aattaatcgc cgactccgta gaaactatgg tgaaagcaca ttgtttcgat
      901 ggtctggttt gcattccgaa ctgtgacaaa attacaccgg gaatgctgat ggcggccgtt
      961 cggctggata ttccgacgat cttcgtatca ggcgggccga tgaaagctgg caggacagct
     1021 ggcgggaaag ccctcgattt gatttcggtc tttgagggaa ttggtgctta tcaagaaggc
     1081 aaaatcagtg agtctgagct ttacgaaatt gaatgctgtg cttgtcccgg ctggggaagt
     1141 tgttcgggac ttttcactgc taactctatg aattgtttgt gtgaagcgct tggaatagcc
     1201 ctgcccggca acggtactat tctggctgaa gatgagcgcc ggattgagct ttataaagcg
     1261 gctggggcaa gaattgtaga actggtgcgt tccaacttgt gtccatcgca aataatcacg
     1321 cgcgcttcta ttgataatgc gatcgtgatg gatatggcaa tgggtggttc gtcgaataca
     1381 gttttacatc tgatggcggt tgcgaatgaa gccggcattg actattcgat taatcgtatg
     1441 gatgaaattt cacggaagac gccgacgata tgtaaagttg cgccttcttc accttatcat
     1501 attgaagatg tagatgcggc gggtggtatg agcgctctgt tgaacgaaat tttccgggaa
     1561 aacgaatttg tcaataagga ttgtttgacg gtgaccggaa aaaccattgc ggaaaacgtc
     1621 gccggtaagc gtattataga cgactcggtt atccgccatc tggaaaatcc ctactcgccg
     1681 accggtggtt tggcgattat gaccggaaat ttagcgccgg aagggagcgt cgtcaagtac
     1741 gccggagttt cggataagat gaagatattc gaaggcccag cagttatttt tgaaagtcag
     1801 gaagaggctt gcgaaggaat tctatccggc aaagtcaaag ccggtgatgt ggtcgtcatt
     1861 cgctatgaag gacctcgcgg tggaccagga atgcaggaga tgctttcgcc cacttcctat
     1921 attatggggc gtggtctggg tgaaagtgtg gcattgatta ccgatggaag attttccggg
     1981 ggaacacgcg gagcttgtat cggccacgtc gccccggaag ccgcagtcgg tggaccaatt
     2041 gctttactta aaaacggtga tcgtattcgc attgatattc cccagaaatc gctggaggtt
     2101 ttactttctg attcagaatt gcaaagtcgt cgcgcccaat ggcaggcgcg tccgccacgc
     2161 ataacgcatg gagtaatggc tcgttatgcc gaaatggtgg cttctgccag tcagggagcg
     2221 gtcttgaaat ataactcacg cataaaagga gaaaagttgt aatgaaaggc gccgaaattt
     2281 taattgatgc cttaattaat gaaggggttg atactatatt tggttatccg ggcggcgtag
     2341 ttattccgat ttttgatgta ctataccgaa ccgataaaat cagattcatt ctaacccgcc
     2401 atgaacaagg tgcgactcac gccgcggatg gatatgctcg cgctaccggc aaagtcggtg
     2461 tctgtctggt aacttccggt ccgggcgcca ccaacacgat cactggaatt gccaatgccc
     2521 gaatggatag catcccgatt gtagtcatca gtggtcaggt ggataccgct aatatcggca
     2581 ctgacgcttt tcaagaaacc gatatgatcg gattgactcg ttccatcact aagcataatt
     2641 atttagtaaa aagcgtagaa gaattgccga tcgttcttaa agaggctttc tacattgcgc
     2701 gcaccgggcg acccggtccg gtggcaattg atatcccggt taatgtttcc aatgcccaac
     2761 tgaaaaatta tcaatatccg tctgaagtta ctctgccggg gtacaaaccg gtgactaatg
     2821 gcaatcccaa gcaaatcgaa aagttagcgc aagccattaa aactgctaaa aaaccactgc
     2881 tttacaccgg tggagggatc atctcggcta atgctgagcc ggaactgctt gccctactcg
     2941 ataaaactaa tataccgatt gtgattactc tgatggggtt gggttcaatt ccatccgatt
     3001 atccgacttt cctcggaatg cccggtatgc acggtacaat ggccgctaac ttggctttaa
     3061 acgaatgcga tttgttaatc ggaattggag tccgctttga tgatcgcatc actggcaaac
     3121 gcgaacagtt tgccaggcaa gccaccgtag ctcatattga cattgatcca gctgaaattg
     3181 gaaaagttat caagccgcac attccggtcg taggtgatgc taagaaaatt ctcagtgaat
     3241 tggtgaaaaa agttagtccg cgtccgaaag acgaatggaa tgaacgcact cttgcctgga
     3301 aaaatcagta cccgatgact tacgatcaag atgcggagga agctattttg ccgcagtacg
     3361 taattgaccg ggttagtgcc ttggctgatc cggatgcgat tcttgttacg gatgtcggcc
     3421 agcatcagat gtgggcggct caatattatc ggcatcgtaa accgcgctcg tttatctcat
     3481 ccggtggatt ggggacgatg ggatttggct tgccagccgc aatgggtgcg gcagttggtc
     3541 gtcccgggca caccgtcatt accatttcag gtgatggcag tattcagatg aatattcaag
     3601 aattagctac ctgttccatc aataaaatcc cggtcaaagt aattatttta aataattcct
     3661 atctgggtat ggtgcgtcaa tggcaggagc tgttttggga cgaacattat tcacagacct
     3721 gtttgaagcg cagtcctgat tgtccgccac aatgtactgg cccacgcaag gattgcccat
     3781 cagtttatat tccagatttt gtcaaacttg ccgaagctaa tcggatttat ggctatcgag
     3841 ctactaagcc cgaagaggtg gatgatgtat taaagcaggg actgagccag cctggtccgg
     3901 ttattatgga attcgtcgtg aaaagcatcg aaaatgtcta tccaatgatc ccatctggca
     3961 agacgatttc agatgttatt ccagggaggc aatcatgatc gaaaaacata ctatcattgt
     4021 taccgtagtc aatcgagttg gtattttggc ccgaattact ggcttattgg ctcagcgcgg
     4081 ttataatatc gaaagcgcta tcgccgctcc aactgaagaa aaggatattt ataaaatcat
     4141 tttagtcgtc aaagaaacca gtgagaatat cgagcaaatt accaagcaac tcaataagct
     4201 gattgataca atcaaggtga ccgatatttc ccataaagat aactatattg tgcgcgaata
     4261 tataattttg aaagtcggtc ttactcctcg cactcgggca gaagtgctgg atttgatgcg
     4321 agtttttggt gccaagccga ttgatttagc cactgatcat ttgattatgg aattagccgg
     4381 acacgtccgc aaaatcgatc ggtttatcga gttacttaag ccatttggaa tcgaggaata
     4441 tgtccgcagt ggtgaatttg ctatgacaga atataattaa taaaaaagga gttagctatg
     4501 tcagtcaaat tgttttatga gaaagatgtt aaaagtgatt ccctgaagaa aaaaaccatc
     4561 gccataattg gctatggcag tcagggacac gctcaagccc aaaacttgcg tgacagcggg
     4621 tacaaagtta ttattgctga aatgccgagt tcgccaaatt atgccttagc cgttaagcat
     4681 ggtttcaaac ccgtcagcgc ggctgaagcc agtgaacaag ctgacgtcat tcaaattctg
     4741 acgccggacg aggttcagaa gcgcgtttac gaacaggaaa tcaaacaaaa tctcactaaa
     4801 ggcaaagccc tggttttttc tcacggattc aatatccact ttaaacaaat tgtaccgccg
     4861 gccgatgtgg acgtttatat ggttgccccg aaaggccccg gtcatttggt tcgccgtcaa
     4921 tacgagattg gtgctggcgt accgtcgcta tttgcggttt atcaggatgt caccggtcaa
     4981 gcgaggaacc tagctctgga acatgcttgt ggtatcggtg ccggcagagc cggaatgatc
     5041 gaaacaacct ttcgcgaaga aactgagact gatcttttcg gggaacaggc ggtcttgtgc
     5101 ggtggcgttt cggaattgat taaagccgga tttcagactc tggtggatgc cggttaccaa
     5161 cccgaaatcg cttacttcga atgttgtcac gaacttaaat tgatcgttga tttaatttat
     5221 gaaggcgggt tagccggtat gtattattcc gtcagtgata cggcagaata cggtggaatg
     5281 acccgtggcc cgtacatcat tgattgcgaa accaagaaac ggatgcagac tttactgcgt
     5341 gccatccaga ctggctcatt tgccaaggat tggattttag aaaatcaggc tgggcgtccc
     5401 acgctcaatg cctaccgtca ggaacatcag gaattattac tggaaaaagt aggtcgggaa
     5461 ctgcgcgaga tgatgccctg gcttaagaaa aaaggataaa atatgaaccg taaaatccag
     5521 atttacgata cgactctgcg cgacggcacc caggctgaaa ctatcagttt ctctgctgac
     5581 gacaagttgc atatcgctaa gcgactggat gaatttggga ttgattatat cgaaggcggc
     5641 tggccgggct cgaatcccaa agatatggaa tttttcaaac tggctcgcga gactgaattt
     5701 cgccacgccc gcctagtagc ttttggttcg actaaacggg ctggtaatgc catagaagat
     5761 gatcagaata ttcacaattt gcttaaggcg gaaactctgg cagttgcgat ttttggcaaa
     5821 acctggattc tgcatgtcga aaaggctctg cgtattacac cggcggaaaa tctcgaattg
     5881 atccgcgaaa gcgtggctta tttaaaaagc aagggcaaag aggtaatcta cgacgctgag
     5941 catttcttcg acggttataa agataatgcc gattatgcta ttcaaacttt gcaaaccgct
     6001 caatcggctg gcgctgattt aatcactttg tgtgatacga acggtggtac tttaccttca
     6061 gaaataagtc gcatcatctc cgaagtgcgt cagaagatca ccgcgcctct gggcattcac
     6121 actcataatg acagtggtct agcagtggcg aatagtttag cggctgtcga agccggatgc
     6181 gtcctggtcc agggcaccat caacggctat ggcgaacgca ccggcaacgc cgacctgtgt
     6241 tcgattatcc cgaatctaca gttgaaagcc ggttatcagt gtatcccgtc ggaaaatatg
     6301 aaacagctga ctccgctttc gcgatatgtc agtgaaattg ctaatctggc tcaccctaat
     6361 gatttgcctt acacgggtag aagcgctttt gcccataaag ggggagtcca cgtcagcgcg
     6421 gtgatgaaag atccgcgcag ttatgaacac atcgagcccg aaagcgtcgg caatactcgt
     6481 cgcgtgctgg tctcggattt atccggccaa agtaacatca tcttcaaggc gcaagaactt
     6541 aatattgatc tcgaacggca tcaggataaa attcctgaaa ttgttgccaa attaaaggaa
     6601 cttgagcatc tcggttatca atacgaagcc gcagaaggtt cattggagct tttgatcagc
     6661 aaaattaaat ccgaatatca cgattttttt aagctgaaag gattccgcat ccttctcgag
     6721 aaaaatgagg atggcgaaca acgctcggaa gccacgatcc gtatggaggt gaataatacc
     6781 atcgaacata ctgcggctga aggcgatggt ccggtcaatg ccctcgataa tgctctccga
     6841 aaagccctga ctaaattcta tcccgaaatt gccgaaatga aattgtcaga ttacaaagtg
     6901 cgcgtattga atccccagca agcgacagcc gccaaggttc gtgtgctgat tgaatcttcc
     6961 tccgatggtg aaacctgggg caccgtcggc gtttcggaaa atatcatcga agccagctgg
     7021 caagccctga cggagagcat ctcttattat ttaatgaaaa aacgtcaaaa aaattccaaa
     7081 tagacggagg ttaatatggc taaacgcatt ataatttttg atacgactct gcgggatggc
     7141 gaacagagtc ccggcgcatc tttgacggct tctgagaaat tgatcatcgc tcggcagtta
     7201 gccaatctcg gcgtggacgt catcgaggcc ggatttccaa ttgcctccga aggcgacttc
     7261 gaagccgtaa aacagatagc gcaaactgta gaaggtccga ttatctgcgg tctggcacgc
     7321 actaacgaaa ttgacattga tcgttgctgg gaagctgtta aatatgccgc caaaccgcgc
     7381 atacatactt tcatcgctac ctctaatatt caccttgaga aaaaattgca taagaccggg
     7441 gctgaggtaa ttgaaatggc ttattcagct atccgtcacg caaagcaata ttgtgccgat
     7501 gttgaatttt cacccgaaga ttgtgcccga actagcctcg aatatatgtg tgaaatcgtg
     7561 caagccgccc tggaagccgg cgctacaacc attaacattc ccgatacggt cggctatgct
     7621 gaaccggagg aatttgccga acggattcgt tacctgattg ataaggtgcc ggcaattcgg
     7681 caagcggtta tcagcgtcca ttgtcataat gatctgggca atgccgtagc taattcgctc
     7741 gccgctatcc gggctggtgc tacccaagtg gaaggctgta ttaatggaat cggcgaacgc
     7801 gccggaaatg ccgcacttga agaagtcgtt atgaacctgg ttacccgcaa agattatttc
     7861 gatgccgaga ctgggatcaa tacccgcgaa atcttcaaga ccagtcgttt ggtggccaat
     7921 ttgaccggtc tgagcattcc gcgtaataag cccatcgtcg gacaaaatgc tttttcacat
     7981 gaagccggta tccatcagca tggcgttctg agcagtcggc agacttatga aattatgacg
     8041 ccggaagatg tcggttggga aggcgaaaac ctggtgattg gcaaacattc cggtaaacat
     8101 gccgtggcgg ctttactgga agcggaaggc tatcgccctg atccggaaca gctcaatcaa
     8161 attaccaaca aagtcaaaga cttggctgac aaacaaaaag aagtcgccgc cgaggatatc
     8221 ctggctattg cccgcgatgt aactatgcaa ttgccggaag aagagcaaca tatccaactg
     8281 gaagaattta ccgtcacgac cggtagtggc ttgacaccaa ccgcctcggt gcgcttggtg
     8341 gttaatggcg aaaggaaaat cggcgcggct accggcgatg gtccgattga cgctgtcagc
     8401 aatgctatct ggagcgtaat agggccctct ctccaattgg tggaatatag tctgaaagcc
     8461 atcaccggcg gaacgaatgc tttagccgat gtttcgatca aattggctga ccagaagggg
     8521 aatatctttg ccgcccgcgc cgttaacgag gacgtcatta aagccagcgc tatgggaatt
     8581 gtcaaaggca ttaataaagc gcttagtagt aaacagaaaa tccatcccca cacggtctga
     8641 attaataatt tggagaataa attgggcgca accataactg aaaaaatttt agcaacgcat
     8701 agcggccggg aacacgtcaa gcctggtgaa ttggttgatt gcaaaattga tacggtaatg
     8761 tgtcacgatg ttactaccac accggcgatt gaactgctgg aaaaatacgc tattccaaaa
     8821 gtcttcgatc cggcgcgcat cgttattatg cctgaccact tcgtacctaa taaagacatc
     8881 aagtcagcgg aaatggtggc tcgcattcgc cgctgggcac aggctcaggg cattactcaa
     8941 ttttacgata ttgggtgtca tggcgtctgt catgcccttc tgccggagca gggttttgtg
     9001 acgcccggga aaacaattgt atgcggcgac agtcatactt gtacgcatgg cgcactgggc
     9061 gctttcgcta ctggtattgg tagcaccgat ctggcggcaa ctttagcgac tggtaaactc
     9121 tggttcaaag tgccagaatc gatgcggttt gttgtttcag gacgcctggc ggatggcgtt
     9181 tacgccaaag acattatcct gaaaattatc ggacaaatcg gtgtggatgg cgcgctgtat
     9241 cgcgctctgg aattctgtgg tgaagtaatt gatgaactat cggtcgaagg aagaatgaca
     9301 atttgcaata tggcgattga agccggtggc aaatccggca ttattaatcc cgacgctaaa
     9361 acactggagt atgtccgggc tcgcactagc gacgaattta ctgtttatcg tagcgatagc
     9421 gacgccgagt actatcgtac aatagaaatt gatgtttccg atttagaacc gctggtggct
     9481 ttgccgcatt tgcccagcaa tgtcaagcct gtacgcgagg tgtccgctat tccaattgat
     9541 caggcttatc tcggcagttg taccaatggt cgaatcgagg atttgcggat cgccgcccaa
     9601 attttgaaaa atcgtaccgt cgcgcgcgga acacggatga tcgtcgtgcc ggctaccact
     9661 gaaatctgga aacaggcaaa ccgcgaagga ttactggata ttttcatgtc ggcaggagct
     9721 accgtcagca cgccgacttg tggcgcctgt ctcggcggat atatgggaat cctggcggct
     9781 agcgaacgtt gtatcagctc gaccaaccgc aattttgttg gacggatggg cagtccgaaa
     9841 tcggaagttt atttagccag tccggcaacg gtagcggctt cggcgatcac tggcaaaatc
     9901 accgacccgc ggtcatattt gtaggagttg attatgcaag gaaatgctca cgtttatgcc
     9961 agagatcaca tcaataccga tgaaattatt ccggcgcgct acctgaatat tgaccgagaa
    10021 gccgaattag ctgagcacgc tatggaagat attgatgtca atttcataaa aaaagtcaag
    10081 cccggcgact ttattgtcag tggcgacgat tttggttgtg gcagttcccg cgaacatgcg
    10141 gtttgggcgt tgcgtggggc tgggattaaa gctgtaatag ccaattcgtt tgctcgtatt
    10201 ttttaccgta actgtatcaa taatggtttc ttagcgattg aatgcccggg cgcggtgcgt
    10261 aaaatttcgg atggcgatcg cctcgaaatt gacttagccg gtggcaagat tgtcaatctc
    10321 acaaagaacc agacttatca atttcaaccc ctccccgatt ttgcccggaa aattatcgaa
    10381 gcgggagggc ttatggaaca gattaaaaag gaacttgctt agatggaagt aaagaaaatt
    10441 gccgtgttag ccggtgatgg catcggtccg gaaataatga atgaagctct gaaagtgctg
    10501 aacgctgttc aacagaaaag cgcaattcga ttcgagttcc gcgaagcgct cgtgggagga
    10561 gcggcttggg agaaatacgg tcagcattta ccggaggaaa ctctccagat ttgccgcaat
    10621 agtgatgcga ttcttttcgg cgccgtcggc gggccagtag aggaacagtt tagcccaaaa
    10681 tggcgaggcg tggaagtgaa cgccctcctc ggtttgcgca aaactttcga tttgtatgcc
    10741 aacctgcgtc cggctaaact tttccccgcc ttagtatttg cctcacctct ccgccaggat
    10801 attgccgaga agggatttga tatattggta gtgcgcgaac tgaccggcgg aatttatttt
    10861 ggccagccga aaggccgacg cggcaatggt gctgaggaag tcgctttcga tactatgatc
    10921 tattcccgcc gcgagattga gcggattgcg catgtggctt tcaaagccgc tcgtcaacgc
    10981 cgacgacacg ttaccagcat tgataaagcc aatgtattga cgacgatggt tttctggcgg
    11041 gaagtggtca cagaagtcgc tcaacagtat ccggacgtta cctgggaatc aatgtatgtt
    11101 gacaatgctt ctatgcaatt ggtcaagaat cccgctaagt tcgatgttat gctttgtgga
    11161 aatatgttcg gcgacattct ttccgatgaa gccgccgcaa ttgccgggag tctgggaatg
    11221 ttgcccagcg ccagcctggc ggaaagtctt tttgggttat acgaaccggg tggcggttca
    11281 gcgccggaca ttgccggaca aaatagcgct aatcccatcg cccagatttt aagtgcggca
    11341 atgatgctga cctattcttt tggaatgaat gaagccgcaa tggctatcga acaagcggtt
    11401 tccgatgtcc tgcaagccgg ttatcgcacg gctgatatct ggcagacagg ttgtcataaa
    11461 attggcacag ctgaaatggg cgaccgcatc gttaccgagt tattaaagaa aattaatcct
    11521 gaataaaaat atttataatt atagagaaac gctcattaag gaatgttatc agaatgagga
    11581 gtaaatttta attggatgtt gattttataa acaattttta ccgcgaattt gctgaacgag
    11641 ttagccgatt aagaagcgaa cttggccggg cgctaagctt tgcggaaaaa actatatacg
    11701 gacacttgtt ttccgaacaa cctcaatcga aattgaaacc ccgtgaatcg tatgtgaacc
    11761 tgaggcctga tcgggtcgct atgcaggatg cgaccgctca aatggcatta ttgcagttca
    11821 tcaatgccgg tcgccgacaa gtagccgtcc cgactactat tcactgtgac catctaattg
    11881 tggcaaaaga cgacgcagtt actgatttaa aaagggcgcg tcgagaaaac cgagaagtct
    11941 atgaattttt gcaaacagcc gcctacaaat ttggtattgg tttctggccg cctggtgaag
    12001 gcataattca ccaaattata ctggaaaatt atgcctttcc gggtggcttg atgatcggag
    12061 ccgattcgca tacgcctaat gccggtggct taggaatgct cggcattggc gtcggtgggg
    12121 ctgacgccgt agatgtgatg gctggcttgc cgtgggaatt gaaaatgccc ggcttgatcg
    12181 gagtcgagtt aaccgggcgg ctgaatggct gggcttcgcc caaggatgtg atcttgaaat
    12241 tagtcggcat gctgggcact aaaggtgcta ccggtatggt tatcgaatat tttggcgatg
    12301 gcgcggctaa tctttcctgt accggcaaag ccacaatttg caatatggga gcagaatgcg
    12361 gcgcgactgc ctcaatattt ccttatgatg ctaaaatggc ggagtatttg actctgaccg
    12421 gaagagcgga ggtcgcaaaa gccgctacgg cggatttcaa ctcaattctc caagctgatc
    12481 cagaagtcta tcaaactccc caaaagtact tcgatcgagt gatcaaaata gacctggata
    12541 atctggaacc gtatgtcaac ggaccttttt cacctgatat ggcagtaccg ctttcacagt
    12601 taagagatca cattaagaaa agcggctacc cagctcattt atcagctggc ttaatcggtt
    12661 cttgcaccaa ttcttcctat gaagatttgt cccgagcggc agatgtcgcc cgtcaagcaa
    12721 atcgtcataa aatcgctacc cgagcaaaat tattagtcaa tccgggttcc caatgggtca
    12781 agttggtttg tgaaaaagcc ggttttctga atgaattcag aacgttaaaa gctcaaattc
    12841 tagcgaatgc ctgcggaccg tgtattggtc aatgggaacg ggatgattgc gagccgggaa
    12901 taaaaaactc tattatcacc agttttaatc gcaattttgc cggacgaaat gacggcaacc
    12961 agcaaactca taccttttta gcttcgccgg aaatcgtgct ggctctaaca ttggctggcg
    13021 atttaaactt taatcctttg actgactttc taatcagcga agacggcaag cgttaccagc
    13081 tcgaaccacc tgaaggtgag gaactgccag ccgtaaaaat ccagaccagc cgcactccgg
    13141 caagaattat tcataaaccg gatattacga ttaaaattaa tccgctctcc cagaggttac
    13201 gtttactgga accatttccc gcgccaacca gccagtcttt ccagaatttg ttgcttttaa
    13261 tgaaagtaaa gggtaaatgc actaccgatc atatttcacc agctggtgaa tggctgaagt
    13321 accgcggcaa tctggacaga atttccgata accttctgct gggtgcccgt aatgccttta
    13381 gcggaaaatt gggcgaaacc cttaaccagt tgaccggcga atatgccaag gtggctgatg
    13441 tcgctagaag ctaccaatcc caccagaaat tatcggtaat agttgccgat gaaaattatg
    13501 gagaaggctc atcgcgcgaa catgccgcga tggagccgcg ttatttggga gtagtagcgg
    13561 taattgcgcg ctcttttgcc cgcattcacg aaaccaattt gaagaagcag ggagtcctgg
    13621 ctttaacctt tacccaattc gatgattatg aacgtgttcg ggaagacgac gaatttagta
    13681 ttcaaagcct tgaaaaattt gctcccgaac gtccgctgga tttgattatc caacataaga
    13741 atggagaaag tgaaacaatc caattacagc atagctacag ccagttacaa atagaatggt
    13801 tttgggctgg cagtgccttg aactatctta aattaaacaa tccacagaag ctctgatttt
    13861 agggaggctt aatatgtcca atgaacaaat agttttcgcc aatggttcgc ttcaggtgcc
    13921 aaattttccg ctgattcctt ttattgaagg cgacgggatt ggagctgaaa tttgccgacc
    13981 cactctgcgg ttgattgatg cggctgtcaa tcgggcttat ggcgctaacc ggcgcatcat
    14041 ctggcgcgaa gttttagcag gcgataaatc tttgaatcaa aatggtgaag cattaccagc
    14101 cgcaaccctt caagatttga atcaatatgt tatcggcttg aaaggtcctt tacggacgcc
    14161 agttgctgaa gggcgccgct ctttgaacgt tattttacgg caaactcttg atctttatgc
    14221 ctgcgttcga cctctgcatt ggtttccggg tgtgcctacg ccagtcagac gaccggagct
    14281 cttgaacgtc gttatttttc gagaaaacac ggaagacctc tataccggaa tcgaatttat
    14341 gcgcggctcg ctagaagctg gcctgctgga agatttcctg attggtaaat tgaaagcgcc
    14401 ggagcctcgc ttccctggcg aaaactcttt aggagtcaaa ccggtttcta aacccggttc
    14461 ggagcggctt attcgagcgg cgattaactt tgccattaaa tataaattgc cgtcggttac
    14521 gctggttcat aaaggcaata tcatgaaata tacggaaggt ggctttcgca attggggata
    14581 tgagctggct gaacgtgaat ttgcggaatc ttgcatcacg gctaagagtg gcgctccagc
    14641 tggatcatta acctcaggat ccggcgacaa agttctgatt aaggatatga tcacggatgc
    14701 gttttttcag gatatattgc tcaacccggc tcgttattcg gtaatagcta ctctgaattt
    14761 gaacggtgat tatatctccg atttggcggc ggcaatggtc ggtggaatcg gtctggcgcc
    14821 gggtgcgaat atcaattttg aaaccggccg cgccattttt gaatcaactc acggcacagc
    14881 ccctgatatt gccggtcacg acatcgccaa tccatcctca ctgattatgt cgggagtaat
    14941 gatgctggaa tatatggggt ggtcagaagc tgccaacctt ttgcaaaagg ctatggcaaa
    15001 cttatttatt cgtcagattg gtactgttga tattttcggt aatgtcaacg gcgctcgttg
    15061 tgtaggcacc accgaattcg ttgatttatt agaagatgaa atcctgaaaa taaatataaa
    15121 gtagcgatta aaaggaactt aatatgtcaa attcaatgtt acaatccata agccaaaaaa
    15181 tgcaaaaagc cagtcagata gacccggaat tatatgtcaa gtacaatgta aaaagaggcc
    15241 tgcgtaacga agatcacacg ggagttctgg tcggattaac ccgcatcggc gatgtagttg
    15301 gctatcgtac ggaaaatggt caggtaattc cgatcgaagg ccagttgctc tatcggggct
    15361 atcaactgga tgatttggtt aaaggctttc aggccgaaaa tcggcatggc tttgaagaaa
    15421 tctgctatct gcttctggcg ggcgaactgc cgaatcgttc cgaactgcaa tatttttccg
    15481 attatctagc taaacagcgc gaattgccag aaaatttcac taaccaaatg ctgattaatt
    15541 tggttagtgg tgatatgatg aatatgctcg ctcgggcggt tctggcgtta tataccaacg
    15601 atccccaacc ggaagatcga agtgtagaaa atcttatcag tcaatcgcta agtctaatcg
    15661 ccaaatttcc gaccatagta gcccattcgt ataatgctta ccgacatgcc tatcagcaaa
    15721 aaaccttatc tattcgtcac ccgcataaag atttaagcat agccgaaaat tttctatata
    15781 tgcttaaagg caatcgctat accaaattgg aagcggatat gctcgattta gctttagtta
    15841 cccacgctga acatggcggc ggaaacaact cgacctttac cttacgagta accagttcta
    15901 ccgggaccga tacctactcg gcaatcgcgg cggctattgg ttctctgaaa ggaccttatc
    15961 acggcggcgc gaatcgcaaa gtaatggaaa tgatgacgga tatcaagaaa agtttgaaaa
    16021 actgggatga caggaatgct gtggctgatt atttaatgaa aatattgaaa aaagaagtcg
    16081 gggatcgcgt cggtaaaatc tatggatttg ggcatgccgt ttatactctt tctgatccgc
    16141 gagtgaaaat tttaaagagc aaagctgcag atttggcgac agaaaaaaat cgtcttgagg
    16201 aatttcatct ctataattta gtcgaagaac ttgcgcctca ggtcttcgcg gatttcaagg
    16261 gacgcgaaaa atctaaaatc gtctgcgcca atgttgactt ttactcggga tttgtctatt
    16321 cttgtatcgg aattccgccg gagctttata ctccgatttt tgccgtcagc cgactggctg
    16381 gttggtgcgc ccatcgtctg gaggaactca atccgcagtc gcgccgtatc attcgaccgg
    16441 catataaaaa tgttactcca gaacgtgagt acgtgaatat agcagagaga agataaaata
    16501 cattattcaa tggttactca acattcgttg gtagtcattt gtggttattg attgcgatac
    16561 gccgttgttt acaggatgaa ctttggctgt atcagcaacc aattttgccc ggcggataaa
    16621 atcggtttag aagcattcga tgggtaaaaa taaaaccctc gtagtagaaa accacttcag
    16681 ggaggatttt taaacggaac tacttcataa gtaatacttt ctgagtagca gaatacctga
    16741 gtgatttcag tctgacgata tacattccag tcccgaccgg ctggccggat tgattgacgc
    16801 cattccagac aactgaatag gttccggcgg agtaatgtgt tttggctgat ttccagactt
    16861 cgcgacccat cagatcgtaa accacgatct cgacgtgtgt ctcttcgggg agatcgaacc
    16921 gaaaagtcgt tttcggatta aacggattgg gataggctcg gtgaagagca taatctgtga
    16981 tagtagaaaa tttgtctcta tgagttccta caataaaatc tgatagtggt cttcgccaaa
    17041 gccccctgat agttccagca tataagtaat catcattaat tttaagttga ttaatattcc
    17101 ccattagact atcactaata tttaggatac ttctgttcag ataatccatt ctaaatactt
    17161 cattgcctga tgaaaatatt aaattatgtc ccctttttaa tgctgacgat atactcttat
    17221 ctggaaaaat attatcccag gttttaccac catcaagaga tcttaataaa ccattcaggt
    17281 tgtaagagct ggattcggta cccataaaca taactgaatc aatcgtcaac acagcattaa
    17341 tttggaaagt atcagcggtg ataaattccc atgtatttcc agaatctttt gatctgaata
    17401 atgccagggt aggcaaatcg tatatatctt tggttacaaa tatattttca tcatcaataa
    17461 aaatatttgt aaacttcccg aatttatcat ctattaaaag tcgatcccat gtataccccc
    17521 catccgtcga tttatatata ttattgtaat aagggatgct atcaatgatg gcatacaatt
    17581 ctgttcctgt tgacctaatc attgacgttt taacattacc tactttattc catatatctt
    17641 cattttcatt ataacaaagt acactggtat cgtttcgaca tccggcgatt atttggttat
    17701 ttccaaaact tgcgaaagtt gagagggata actctggatc agtgatgatt ggtttccatg
    17761 aatttgtact cattgagtaa atatttccaa gataaggagt gggtggtacc gaaaaagtaa
    17821 atatactatt tccacaaaat tcaaactctt ctaccgatct tgaggtgatg ccactcaaat
    17881 ccatgtaaga ccaattgaca ccatcaatag tggagtatat catacctctt tccgttgcta
    17941 catatacacc tgaactgtca atgtaaatat ccctatatat ctcagaggaa tctattccta
    18001 gtttaagctc tgtccaatta tttccactat cagccgagtg gaacaattga ctgatatttg
    18061 taaaatataa atgcgagccg tagtaaacta atttattggc gcctccagtc gatccaagat
    18121 caggagaaga taacttggtc cacgaatccc catcatcaga ggagatataa aaatcataac
    18181 cagtgtttaa gattaaatta gtcccattcg aggcgataca atatatcaca tgaattcctt
    18241 cataaatttg atcccaagac acacaattat ctgtagatcg gtacaggcaa ttctcagcag
    18301 ataagaatat tgctgaatct gaataagcaa tatcccatat ctgtctaccc tcaggcagat
    18361 tattgatatc agaccttgac caatttgcac cattatcttt tgagaaaaat aaaccggcgt
    18421 aaggattagt gactattacc atggaatcct taatacttat acgttggacg tagacagaat
    18481 cgaaaatgcc attgatactt tcccaggttc taccataatc cgatgaatgt attaaacaag
    18541 tatggttatt tgttgggtag ccaaatgcca caaagatatc agaatcttta gcggcaacat
    18601 cataataatt ggtaacgtcc aagtatgtat tatcccaggt aattcccatg tcattcgatt
    18661 gatataaata gtacttggat aacaggcgtg tagctgagac tgcatacatc gtaggattat
    18721 cgcctgtaac tccaagtatc attccaccgt acaatccttc tattctttcc cattgtgcat
    18781 ttacattttc aataaaagta acaaataaga atatactcaa taaaaataac ggtttttttc
    18841 tcatgattgg ctcctttcaa aaaatgttaa tgacttgcta caatttaatg ccatatagtc
    18901 ttctttgcca gacttcccat ttaaattgag aataaaaaaa tgcttggcat tttgttgtgt
    18961 gataaccata ccgtttgact tcgaggcata gcattaattt ttcccattct acatggatta
    19021 tttagccaat attgataaag tcaaaataat ttgaggtatt tcatgtataa atcctttcat
    19081 tttatatgat gccctctgat aataaccgca cttgaataag atccaagaga accaactaga
    19141 acgagttggt tacctcttat agataaatta tataaactta tctctggaaa ctgctccatt
    19201 aataaagtaa tgtgagccca accatttcca ttataatgga aaagttctcc ccagatcgtc
    19261 atacatatta tgtcgttgta acttgtcccg cttattttcc ataaccgtcc gtccataata
    19321 aagtcactat taatataacc gtggatcaac tttccttttc ctgtcttgat agattctttc
    19381 cataatccgg aaaaagcagg aatatacact gtgtctctat aagcccaaac cgaagggctg
    19441 aggtatcccg atagtttagt ggggctgtaa acaaatttat ctcctggaga tgccgtatat
    19501 ttccgtcgcc aactttcgcc attaaaatga aaccatatat cctcataacc aaagggatga
    19561 ttgtcatcga tgatgttggt cacggctatc gcccagatat tatccttatt aattccccaa
    19621 atgtcaacta taggcgagtc tgttccactt tccatcttcc tgaaattctt gccatcgtag
    19681 tggacaatcg aacccttata ccctacgaaa ta
//
LOCUS       DAOMJU010000035        19559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2614_length_19559_cov_16.306245, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000035 DAOMJU010000000
VERSION     DAOMJU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 19559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19559
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2614_length_19559_cov_16.306245"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1062
                     /gene="trpS"
                     /locus_tag="PLO58_04950"
     CDS             73..1062
                     /gene="trpS"
                     /locus_tag="PLO58_04950"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQO74171.1"
                     /translation="MENRKVKGRILSGMRPTGKMHLGNYVGALENWVKLQDEYENYHL
                     IADYHALTTNLDTSELFSNTIEMVYDWLAVGIDPQRSPIFRQSQVKEHCELFLIFAML
                     ITTARLERNPTLKEQVRDLNIGTLSFGHLGYPVLQAADILIYKANLVPVGEDQVPHVE
                     ITREIARKFNQTWGKVFPIPEAKLTKFARLPGLDGNKMSKSVGNTILLADSDEEINSK
                     IKKAITDPQKIRRGDPGRPEICLVYTYHEKFNPAQCPEIYAGCTSGALGCVECKMKCG
                     KAIQSVIAPYRTRRMYYATHQSEVIDILIDGEKRARQVAQNTMADVHNKMRIG"
     gene            1116..1856
                     /locus_tag="PLO58_04955"
     CDS             1116..1856
                     /locus_tag="PLO58_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQO74172.1"
                     /translation="MSYNVKLNVYEGPLDLLLFFIKRDEINIYDIPIAYITDEYLNYL
                     QLMQSLNLQLAGEFILMASILIRIKVQMLLPRPETEKLEDIEDPRTELVQMLVEYQKY
                     KEIGEQLKRLEANQSRHFPVTLTSDQRNYDPELFLGDITLIDLGLAFQQILEKLPKVE
                     PVNLEIMKVNIRQQILFIRECLKKRSRIRFSEIAAKLTDRLEVVVTFLAILEMIKSME
                     ISVSQKDIFGEIWITKKKTIQDNVISNA"
     gene            1837..2397
                     /gene="scpB"
                     /locus_tag="PLO58_04960"
     CDS             1837..2397
                     /gene="scpB"
                     /locus_tag="PLO58_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQO74173.1"
                     /translation="MLSQTHRIVLALLFGATEPLTKSQVDICLEEDIDLTQIIADLNQ
                     YLETEGLPVWIQLVAGGYRLMTRAEYEPWLSRLFQNKGKLRLSRSALETLAIIAYKQP
                     VTKSEIDAIRGVDTSLRPLVEKNLIKITGRQEGPGRPLLYGTTNHFLEYFGLASLQDL
                     PQVKELAEIAGEDNLQSDIVEQDATE"
     gene            2384..3097
                     /locus_tag="PLO58_04965"
     CDS             2384..3097
                     /locus_tag="PLO58_04965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQO74174.1"
                     /translation="MRLNRYLAQGGFGSRRKCEDLIRGGRVKINGCIVTNLATQVKET
                     DQVTVDNRRIVPPKPVYILLNKPAGYITTMHEQFGRKAVTDLVNNAFAKPIGRLDMAT
                     TGVLLLTNDGELAYRLTHPKFGVERIYRAAIAGRVSPDIKKQVAEGISIGGAQKASGQ
                     ILTIKYLDGFSSVTIELKEGQNREVRRIFAVLGYKVISLDRISFAGVRYSGLERGQWR
                     NLNESEVRRLKTLVGLADQ"
     gene            3221..3940
                     /gene="cmk"
                     /locus_tag="PLO58_04970"
     CDS             3221..3940
                     /gene="cmk"
                     /locus_tag="PLO58_04970"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQO74175.1"
                     /translation="MGIIIAIDGPAGSGKSTTAREVARRLNFTYIDTGAMYRAITLAV
                     IRQNVAVEDHQSVIKIAEQADIQFEWADGNLRTILNGEDVSNEIRSAQVARLVSPVSA
                     IGGVRDILVKRQREMAKTANIVMEGRDIGTNVFPDADFKFYLEADLETRARRRINDYQ
                     KIGQNLSITEIMTEIEQRDKIDSSREHSPLKKAPDAIVIDTSNLKFEEQVEMIIQIVK
                     SSELFKKIEKAPVINGTNCKN"
     gene            3965..>5982
                     /gene="rpsA"
                     /locus_tag="PLO58_04975"
     CDS             3965..>5982
                     /gene="rpsA"
                     /locus_tag="PLO58_04975"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQO74176.1"
                     /translation="MLEEEQTLNPKPSEESEVIPDINPADPFAEPVTARDATIEERTS
                     ATSDYTTELVTSGPLSEIVRVYDLKDIDKLNEVQAPKAEDHTKIYENSLKDIAKDQRI
                     LGTVVAINENEILVDVGFKSEGIITREEFEEGELPKIGDQIEVFVDVLEDENGQMILS
                     KRKADFMRIWERIKEIYAAGETVEGQIVSRIKGGMVVDIMGIDAFLPGSQIDIRPVTD
                     FDSYIGKTFEFRIVKLNELRKNIVLSRKELLEESMKEKRDDLLSKIKVGDILTGRVKN
                     ITDFGVFIDLGGLDGLLHITDISWGRINHPREVVQMDQELTVKVIDYDQEKQRVSLGL
                     KQLTTHPWEGIESKFPVGTIVKGKVVNITNYGVFVELEKGVEGLIHISEISWTQHIKH
                     PSEVFKLGDEIEAKVLGIDSAERKISLGYKQLEPDPWESIEQKFTVGSVHKGIVRNLT
                     PYGAFIELQEGVDGFAHISDMSWTKKIRHPRELLKKDDEVEFKILEISKENRKISLGL
                     KQVTEDPWPMIETLFSVGSIVEGEVAKVTDKFIVVNLEHDLEGIIPLGQMPKKDRKDL
                     SKAVEIGEKLQLKVLEVNRQEKKIILSREQAIPREPKTEVEAYMMQQAETTNKLEIPS
                     EIIQKIAESEEKTQEKEEKSAAKVKSLAKKAKKAEKVEKPEDLTPEEE"
     assembly_gap    5983..6082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6083..7054
                     /locus_tag="PLO58_04980"
     CDS             <6083..7054
                     /locus_tag="PLO58_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HQO74177.1"
                     /translation="QAVRKWITTNTRTKIWIFVFTVFLWLYVVLNNTYSYTFSAKLEV
                     RNIDPTKTTIEKLPSQVQANFSGKGIDLFYLILSPQQSIKFVVDCYSIKQYYDFPLNE
                     YFKNNPEKVITPRNINVKLDHIIWPETLRVVLDDLVTAKVPVKPEVEIQLAPGYVLAG
                     SMRIIPDSVTLRGPRTYVRQYTAVPTKKLVLTNVSSSISQEIDLEFVPKYNVYIDVTR
                     VRILQKVDQLGEKKLTDIPIRVINLAQDQKAEIIPPTATLTISGGIEKLKTVQPEDIK
                     IVFDFATDWRPDESFYTPTVELPPGLLNWWNLEPDTFEIRIIRERQP"
     gene            7055..8059
                     /gene="tsaD"
                     /locus_tag="PLO58_04985"
     CDS             7055..8059
                     /gene="tsaD"
                     /locus_tag="PLO58_04985"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQO74178.1"
                     /translation="MLILGIETSCDETAVAILNDNEILVNLVSSQLLHQQFGGVVPEF
                     ASREHMRLLDQMVKEAFVQTGLQPENLDGIAVTRGPGLMGALLVGLSFAKGLAYSLRK
                     PLLGVNHIEGHIMANFLTCPDLKFPFLCLLVSGGHTQIIQVNDFGNYQILGRSVDDAA
                     GEAFDKAARILELPYPGGPQIDQMAHRGDPNFYQFPRAKVRSEQYDFSFSGLKTAMLY
                     LVRQRGQKWAQENLADLCAAYQEAIVDVLKINTFTAVKNLHANKLVLAGGVAANSRLR
                     QVFQEQADYYQIELIIPSLKYCTDNAAMIARTGQEMLSRNQISPFSISAVPNLTLTTK
                     "
     gene            8061..>9520
                     /locus_tag="PLO58_04990"
     CDS             8061..>9520
                     /locus_tag="PLO58_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74179.1"
                     /translation="MHSLTLKIPGFEWWWFILLLGLVGLLFFSFRKIPGGRSRSVTTI
                     LLVLRSLILILLAGLILKPEIHWTSSRKKAPSALVWIDNSLSIAAQNNFSKDSLLSIV
                     QEINTALDGKGIKPHLFLFDEKIIPVTGKLKNIENNGLATDLAQVLRYSKFEFENENI
                     VGAIIISDGVITRGEDPTFSDIEMPFPVFTIGIGDSELVMDPAVTKIEMPQTVKAGDT
                     VTINAEIIPTGNGDPITVMLKEGEIILDKKVIASQSQAMRKMLSFQAIPSKPGEKIYS
                     VEITAAQDKNPYNNLRMNAVKVLESQIKILIVSGQANFEAPFLTRTLRGLQDVFVQNV
                     VINKGQWLPLSFQEIMAKEWNLIILIGYPTAESSQNDLIRLRQKISDYNLPLIFFLDG
                     DVDSKQLERLLGWNPIDELLADKSVNQISVKPTRAGLEHPLIKNFQKVNVSEIIWREL
                     PPIGSPYRRIRLKPSLLTIIESTDIAENPVIAINQE"
     assembly_gap    9521..9620
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9621..10046
                     /locus_tag="PLO58_04995"
     CDS             <9621..10046
                     /locus_tag="PLO58_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74180.1"
                     /translation="AVVKAELIQQNDVIASSNLIWNGNNYSGVIPVRNAGDCQVRISA
                     WSGDNLIGIREQPITVVDRSIELLEVRQNVDLLRTIALRSGGLKTNRIPEIIDRLQVT
                     EKIITQSHVIKFLNWRWTLVILIVLLTVEWSMRRFQGFL"
     gene            10207..>11459
                     /locus_tag="PLO58_05000"
     CDS             10207..>11459
                     /locus_tag="PLO58_05000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQO74181.1"
                     /translation="MKKIAMLGVGYVGLVSGTGLADFGNEVICYDIDTTKIEKLNNGE
                     MPIYEPGLIDLVRKNVAKGRLKFTDDLTLAIRESEVIFLAVPTPSGENGEVDLSYIFN
                     AVDQIAKVIDSFKVIVTKSTVEVGTNDQIRERLLSHKIAPEMFEVVSNPEFLREGSAV
                     SDFMRPDRIVIGTSSPKDLEIMKEIYRPLYLIETPIVATDIHTAEMIKYASNAFLATK
                     ISFINEIANLCELVGADVQQVAKAMGLDGRIGKKFLHAGPGYGGSCFPKDTLALTKIA
                     EKAGMRSTIVEAVIEANNRQKLRVVEKLLKVLPNLSDKTIGILGLAFKPNTNDVRETP
                     ALTVIEEILKRGGQIKAYDPEAMKEMRKYYPQITYVASTEDAAKGADALIIMTEWNEF
                     RNLDLNYLKSVMKNPIMIDSRNIYDP"
     assembly_gap    11460..11559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11560..12484
                     /gene="rfbB"
                     /locus_tag="PLO58_05005"
     CDS             <11560..12484
                     /gene="rfbB"
                     /locus_tag="PLO58_05005"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HQO74182.1"
                     /translation="VRVINLDKLTYAGNLANLQDLADNSDYIFVKGDICDADTVNNIF
                     RKYHPDYVVNFAAESHVDRSIGKPDDFIRTDMFGVFVLLEASKEFGIERFIQISTDEV
                     YGSILEGSFTEESPLKPGNPYSASKAGGDRLAYSYFNTYNLPVIITRASNNYGPYQYP
                     EKLIPLFVTNLIEDKPVPLYGDGKNVRDWLYVWDHCDAIEFILKNGKPGEVYNIAGEN
                     ELENIFITNFIFEYMNKPKTLIKPVADRLGHDRRYSISAAKLHELGWQAQHKFEDAMR
                     NTIQWYIDNPQWWKPLKSGEYLEYYKRYYKI"
     gene            12496..13734
                     /locus_tag="PLO58_05010"
     CDS             12496..13734
                     /locus_tag="PLO58_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HQO74183.1"
                     /translation="MKKAFVILMVAILFDISLGQQYRSSESQKSYRSYGKMFEQETEK
                     EKTTLKEKAKEVSKMQELIALDKAVSPEEYIVGPGDIFGIDIIITENINIELAITPTG
                     DLLIPSVGKINVNGLILKDAIELCKKKINEVYPSAKVDVALVNLRTFKIQIAGAVFQP
                     GFYDVTAVTRLNEIIELAEGFDQFAKEFNIQITRNDGRIDTINYFNYKLEGDLKHNPT
                     FLEGDRIFVPFGDPDKEAIVIRGAVYGSGYDIIQENESLGYYLRRQAQFIENADLENV
                     TITRKEADHQLVMTVYPRDFFSTPLKPGDAIDILSERGISVNGFVQSPGSFYFYPGYS
                     YLDYINMAGGNTYEGDIDKVYIRHLDGSTEPGKSATLKRGDVIIIPRSSKSTIFGDLS
                     VLEIFTSIATILLTFIAAMK"
     gene            13773..14765
                     /locus_tag="PLO58_05015"
     CDS             13773..14765
                     /locus_tag="PLO58_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HQO74184.1"
                     /translation="MAPIKKEHPLLYFIFKHRVTNLLILLLGAILVFVIVFFGMKKKY
                     TATVSILPPSQDLSSGIGSSMGALANLAGINLPQSTLQSPEMYKEILTSRKVLDTILF
                     EEFEGVDSSGTIYRKKLIDMLDIKAKNEREKIDKALKKLLKDVIYVNINNESNVTKLS
                     VTLTDPFYAANVANRMVELLNEIVVTQIQHENRQQLLYIEDHIKNTQDSIKIAEKYLQ
                     TFLEQVKSIAEPANQIKELALKRDLELYSTIWIELKQQKEIYTLKSIPSLAEVKVLDI
                     AQAPYLKSRPKRLLTMISLGGLFLILQICVNALILIINNFRKELKPDEPIPHPK"
     gene            14740..16047
                     /locus_tag="PLO58_05020"
     CDS             14740..16047
                     /locus_tag="PLO58_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HQO74185.1"
                     /translation="MNQFPTLNELKGFFSNKEGKSLFANFAQFGIYQVIIYLAPLITV
                     PYLLRYIGVAKFGIISFAMALVYYFQIVVEYGFGITGIQMIAQSQNNSRKQSEIVSNV
                     LIIQIILFSICFILLGILCFLIPKIHDYRSIYLFSYGYVLANIFLFTWFYIGVEKVKY
                     VNVIISISKLLYILLIIIFIKNENDFVLVPIFNSGTTFLAGIVSIIILRKKFIIRFLK
                     PNKDILKRYLKEGWHIFLSNIAMNLYRNTNVIILGIFSSDISVGIYSAGEKIVKAIQS
                     TLSPLTQTLYPFISRKKIEEPEKSIRYISRMITIMGFIGCIISILLFIFAGKIAYLLN
                     GSVNLAMVRVIRIASFVILFGGLNYCIGIIFMTNYDMKKEFLKSVMIVGILNTIICAI
                     LCHLISEIGAATALLLSEFILFMLLVYHIQHNKYKCSTNPLEL"
     gene            16044..17108
                     /locus_tag="PLO58_05025"
     CDS             16044..17108
                     /locus_tag="PLO58_05025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQO74186.1"
                     /translation="MSYPHVSIIILNWNKWADTIECLESVLKSDYPNFNIILVDNGSD
                     NDSVEKILAWADGKLIKPYISNYDEIVLPEVKKPLIIQKIQIENINHVPTESGKNAMI
                     NLLISNENLGFAKANNLSIRYADATWNSKYYYLLNNDTVIEKETLRNLVTVLENETLY
                     KIAQSTIFYYNNKEEIACAGGRLFPWGKGKFYTRITSDEIKEIGFINGCALLISQEIV
                     EKYGLLSERFFFGEEDFEYSYRLSLAKVKKVCVGNSRVYHKISVSSNHLLDNDRGRSL
                     VLSALNRIADMRNYYPRFIWIFWRYLTVIYYYLFLMIIKHGIDTKLANKYLKYILKKS
                     RNISSVDKTLYEEIINKKWN"
     gene            17099..18373
                     /locus_tag="PLO58_05030"
     CDS             17099..18373
                     /locus_tag="PLO58_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6337 family protein"
                     /protein_id="HQO74187.1"
                     /translation="MELTKLSSLFFAAVILAMTIFEKRRLNTIITPFSVSAWPLVFII
                     LVVNFMLVKIEFDIVTPRAILFLLANLFIIWLIGIIFSYIHNNDRIQSLNDIFLPYVK
                     FEKILIVLSWVVNIVVFLKMKNLLAKYGGLSFLGDYRFEEMINSGPEAHISQFGKVTF
                     LLLSFIYPMANHKKTILITLFGSIVMLFMLQSKYNLIGVLVIVFIYYNFTKPVKKQIG
                     SILKFSLLTLMIMALSLITLTIFWGTFKVSKQSVWLFLSKNLLSYFFSSPIVLDKWLD
                     HAFVQPDWTLLLLFKNIINRIVGNPLVYSSLDYVDLNFSEVAKDVYSNVGTSYGTYFI
                     IGGWIFSLFMSTVISSLTYFVYYSAKKKPNPVNVFLCMTLLNIALFSFFGQYFTFLPY
                     YEMIIFYIVTIKVMDTYNLFISDYQNSLPLRK"
     gene            18403..19482
                     /locus_tag="PLO58_05035"
     CDS             18403..19482
                     /locus_tag="PLO58_05035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQO74188.1"
                     /translation="MNVFGGAVGRILVDVMPHLVKKFDVKVVSIQSIDYSEPLVQEYI
                     RKYNNLESLKLNRFSLFKAYHKLNCYFKKIDPDLIHSHMGRADIFTAMSKTKNMIIMN
                     TLHCEKKNHNPLTQIGYRLTDRRVDWRICISQTVARSYYNHGFKSPYSVIYNPIDING
                     FTKPINREEIRNNLGFKPNEQIIINVGRLIKCKGQRYLIEVMPHLCHLLPEIKLLIVG
                     DGPQYNSLKKLIINNNLENNVYLLGYRQDVQNLLKISDLFVYSAQWEGLGMAVIEAMA
                     TRIPVLAHPLPALKEYIIDNHNGYFCNIHLKESFANKIYDILTNSHHNSSIAENAFRT
                     VTEMFSADKISKQYIELYQQLLSNR"
BASE COUNT         6272 a         3470 c         3837 g         5680 t
ORIGIN      
        1 atacattaca acgatttgcc gaatcaccaa ttgacgagtg gcggccatat ttatttttag
       61 aataaggaca aaatggaaaa tcgcaaagta aaaggcagaa tcttaagcgg aatgcgcccg
      121 accggtaaaa tgcatttagg taattacgtc ggagctctcg aaaactgggt aaaattacaa
      181 gacgaatacg aaaactatca tctcatcgcc gattatcatg cgctcacgac taatctggat
      241 acaagtgaat tattctcgaa cacgattgag atggtttacg attggctggc agtcggaata
      301 gatcctcaac gcagtccgat ttttcgtcag agtcaggtca aagaacactg cgaactattt
      361 ctaatattcg caatgttaat taccacagcc cgccttgaac gtaaccctac ccttaaagaa
      421 caagtgcgtg atttaaatat tggcactttg tcgttcggac atctcggtta ccccgtgctc
      481 caagccgccg atattttgat atataaagct aaccttgtac cggttggcga agatcaggta
      541 ccgcatgtag aaattacacg ggaaatcgcc cggaaattta atcagacctg gggtaaagtt
      601 ttccctatac cggaagccaa actaaccaaa ttcgcccgcc ttcccggttt agacggcaat
      661 aaaatgagta aatccgtcgg caatacgatc ctcttagcgg attcggatga agaaatcaat
      721 tccaaaatta aaaaagctat cactgatccc cagaaaataa gacgcggcga tcccggtcgt
      781 cccgaaattt gtctggttta tacctatcat gaaaaattta atcctgccca atgtcccgaa
      841 atttacgccg gttgcacaag cggcgctctc ggttgtgtcg aatgtaaaat gaaatgcggg
      901 aaggcgattc aaagtgttat cgcaccttat cgtactcgcc ggatgtatta tgccactcac
      961 caatctgagg tcattgacat tttaattgat ggcgagaaac gggctcgtca ggtcgcccaa
     1021 aacactatgg ctgatgtcca taataaaatg agaatcggat aagacgtgat aatccaatac
     1081 ctggactggc gaatccagca attttaatcg gattaatgag ttacaacgtc aaactaaacg
     1141 tatatgaagg gccactcgac ctcttgcttt tctttattaa gagagatgag attaacattt
     1201 acgacatacc cattgcctat ataacggatg aatatcttaa ttatctacaa ctcatgcaga
     1261 gtttaaatct gcagttagcg ggtgaattta tcctgatggc ttccatcctt ataagaatca
     1321 aagttcaaat gctcctgccg cgtccggaaa ccgaaaaact cgaggatatt gaagatccac
     1381 gcaccgaatt agtccaaatg ctcgtcgaat atcagaaata caaagagatc ggcgagcaac
     1441 tgaagcgact tgaagcaaat caatcccgcc attttccagt cacactgacg tcagaccagc
     1501 gcaattatga tcccgaactt ttcctcggtg atataacttt gatcgatttg gggcttgcct
     1561 ttcagcaaat tctcgagaaa ctgccaaaag ttgagccggt caatctcgaa attatgaagg
     1621 tcaacattcg tcagcaaata ttattcataa gagaatgttt aaaaaaaaga agccgtattc
     1681 gattttccga aattgcggct aaattaactg accgactcga agttgtggtt acttttctgg
     1741 ctattctgga aatgatcaaa tcaatggaaa ttagcgtatc gcagaaggat attttcggtg
     1801 aaatatggat aaccaagaaa aaaactattc aggataatgt tatctcaaac gcatagaatc
     1861 gtactagcgc ttttattcgg cgcaaccgag ccgttgacta aatcacaggt agatatttgt
     1921 ctcgaagagg acattgattt gacacaaatt atcgccgatt taaatcaata tcttgaaacg
     1981 gaaggacttc cggtctggat tcagttggtg gcgggaggtt atcggctaat gacaagagct
     2041 gaatacgaac cctggctaag tcggcttttt caaaataagg gcaaattacg tctgtcacgc
     2101 tcggctctgg aaaccctagc tattattgct tacaaacaac cggttaccaa aagcgaaatt
     2161 gatgcgattc gcggggttga taccagcctc agacctcttg tagaaaaaaa tctgattaaa
     2221 attaccggtc gtcaagaagg acctgggcga cctctgctat atggtaccac taaccatttt
     2281 ttagaatatt tcggattggc atctttgcaa gatttaccac aggttaaaga actggccgaa
     2341 attgccggtg aagacaatct ccaatctgat attgtcgaac aggatgcgac tgaatagata
     2401 tttagcgcag ggcggttttg gttcacgacg caaatgtgaa gatttgatcc gaggaggtcg
     2461 ggttaaaata aatggatgta tcgtaaccaa cctggctact caggtaaaag aaaccgatca
     2521 agtgactgtt gacaatcgcc gaattgttcc acctaaacca gtgtatattc tcttaaacaa
     2581 accagctggc tatataacaa caatgcacga acaattcggc cgcaaagcag tcaccgattt
     2641 agtaaataat gccttcgcca aacctattgg tcgtttggat atggctacaa ccggagtgct
     2701 tctgctgact aatgacggcg aattagccta ccggctgact caccccaagt ttggtgttga
     2761 acgcatctat cgagcagcaa ttgctggtag agtctctccc gatattaaaa aacaggtagc
     2821 ggaaggaatt tccatcggag gggcgcaaaa agcatctggt caaatcctta ctattaaata
     2881 tttagatggt ttttccagcg tcaccatcga attaaaggaa ggtcagaatc gcgaagtgcg
     2941 tcgcattttt gctgttttag gctataaagt catttcactg gatcggataa gtttcgccgg
     3001 tgtgcgctat tcaggattgg aacgtggtca atggcgcaat ctaaatgaat ctgaagtacg
     3061 gcgactgaag actctggttg gtttggcaga tcaatgacgg agtgacaaat ttttacaatt
     3121 gattattcaa atattgtatt aatttacctt aagatagctg aaaggaattt gaattgttat
     3181 cctatggaat taaatttacg cgctttttga aaggtagaat atgggcatta taatagcaat
     3241 cgatggaccg gccggtagcg gaaaaagtac cactgcgcgg gaagtagctc gccgactgaa
     3301 tttcacttat attgataccg gcgcaatgta tcgagcgata accttggcag ttatccggca
     3361 aaatgttgca gtcgaggatc atcaatctgt gataaaaatt gccgaacaag ccgacattca
     3421 gtttgagtgg gcggatggca atttgcgtac gattttaaac ggtgaggatg taagtaatga
     3481 aatccgttca gctcaagtcg cgcgtttggt aagtccggtt agtgccattg gaggcgtacg
     3541 tgacatttta gtcaagcgcc agcgcgaaat ggctaagacg gcaaatattg ttatggaagg
     3601 tcgcgatatc ggcaccaatg ttttccccga tgctgatttt aaattttatt tagaagctga
     3661 tttggaaacg agagctcgcc gacgaattaa cgactatcaa aagatcggcc agaatttatc
     3721 tataacggaa attatgactg agatcgaaca aagagataaa atcgattctt cgcgcgaaca
     3781 ttcacctctt aaaaaagccc ctgatgctat tgtaattgac acctccaatt taaaatttga
     3841 agaacaggtt gagatgatta tacaaattgt gaaatcatcc gaactcttca aaaagataga
     3901 aaaggctccg gtcattaacg gaaccaattg taaaaattaa ccctaaacca aaggaacatt
     3961 ttcaatgtta gaagaagaac aaaccttaaa ccctaaaccc tccgaagaat cggaagtaat
     4021 tccggacatt aacccggctg atccattcgc cgagccagtg actgccagag atgccacgat
     4081 tgaggaaaga acatccgcca cttcggatta tactaccgaa ttagtcactt cggggccatt
     4141 gtcagaaatt gtaagagtat atgatttaaa agatattgat aaattgaatg aagtccaggc
     4201 tcccaaggcc gaagaccata caaaaatcta tgaaaactca ctaaaagata ttgcaaaaga
     4261 tcagcgcata ctcggaacag ttgtagccat taatgaaaat gaaattttag ttgatgtcgg
     4321 cttcaaatcc gaaggtatta ttacccgtga agaatttgaa gaaggtgaat tgccgaaaat
     4381 cggcgaccag attgaagtat ttgtagatgt acttgaagac gaaaatggtc agatgattct
     4441 ttccaagcgg aaagccgatt ttatgcgaat ctgggaacga attaaggaaa tttatgcggc
     4501 tggtgaaact gtagaagggc aaattgtcag ccgtattaaa ggtggaatgg tagtggatat
     4561 tatgggaatt gatgcctttc tgcccggctc acaaattgat attcgaccgg ttaccgattt
     4621 tgattcctat atcgggaaaa cttttgaatt tcggattgtc aaattaaatg aattacggaa
     4681 aaatattgtc ttgagccgta aagaattact cgaagaaagt atgaaggaaa aacgtgatga
     4741 tttattatcc aaaatcaaag tcggtgatat tctgactggc cgggttaaaa atataaccga
     4801 ttttggagtt tttattgatc tcggtggatt agacggctta cttcatatca ccgatatttc
     4861 ctggggacgt atcaatcacc cgagagaagt agttcagatg gatcaggaat tgacagtcaa
     4921 agtcattgat tacgatcaag aaaaacagcg tgtttcgctc ggactcaaac aactgaccac
     4981 ccatccctgg gaaggtattg aaagcaaatt tcctgttggg acgatcgtta aaggcaaagt
     5041 tgttaatatc actaattatg gcgtattcgt ggaactcgaa aaaggggttg aaggactaat
     5101 ccatatctcg gaaatatctt ggactcagca tattaaacat ccttcagagg tgttcaaatt
     5161 gggcgatgaa atcgaggcta aagtgctggg catagacagt gccgaacgga aaatttcatt
     5221 aggatataaa cagcttgaac cagacccgtg ggaatcaatt gaacagaaat tcacagtggg
     5281 ttctgttcac aaaggtatag ttcgaaatct gactccttat ggcgccttta tcgaacttca
     5341 agaaggtgtt gacggatttg cccatatttc agatatgtcc tggacgaaaa aaatccgcca
     5401 tccccgggaa ttgctaaaaa aagacgatga agtcgaattc aagattctgg aaatctcgaa
     5461 ggaaaatcgc aaaatctctc tcggtttaaa gcaggttact gaagatccct ggccaatgat
     5521 tgaaactctt ttttctgtcg gaagtatcgt ggaaggcgaa gtcgctaaag tgactgataa
     5581 attcattgtg gtcaatctag aacacgatct cgaaggaata attccattag gacaaatgcc
     5641 taaaaaagat cgtaaagacc tcagcaaggc agttgaaatt ggtgaaaaac tgcagctcaa
     5701 agtcttggaa gtcaaccgac aagaaaagaa aatcatacta tcacgtgaac aggctattcc
     5761 gcgcgaaccc aagaccgaag tagaagctta tatgatgcaa caggctgaaa cgactaataa
     5821 actcgaaatc ccgagtgaaa taattcagaa aatcgccgaa tcggaagaaa aaactcagga
     5881 aaaagaagaa aagtcagccg caaaggttaa atctctcgcc aaaaaagcca agaaagcgga
     5941 aaaggtcgaa aaaccagaag atctcacccc agaagaagaa aannnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nncaagccgt ccgaaaatgg attaccacca acacgcgtac
     6121 taagatttgg atcttcgttt tcaccgtttt tctctggctc tacgtcgtat tgaataatac
     6181 ttattcttat actttttcag ctaaactgga agtgagaaat attgacccga ctaaaacgac
     6241 gattgagaaa ttaccgtcgc aagtccaagc taatttttca ggtaaaggaa ttgacctgtt
     6301 ttatcttatc ctatcacctc aacaatcaat caaatttgtc gtagattgct actccattaa
     6361 acaatattac gatttccctt taaatgaata ctttaaaaat aatcccgaaa aagtcatcac
     6421 tccacgaaat attaatgtca aactcgatca cattatctgg ccggaaacct tgcgcgttgt
     6481 tttggacgat ttagtcacgg caaaagtacc ggtcaaaccg gaggttgaaa tacaattagc
     6541 acctggctat gtcttagccg gttcaatgcg tataattcca gacagcgtta ccttaagagg
     6601 tccgcgaact tatgtcaggc aatatactgc agtgccaact aaaaagctag ttctcactaa
     6661 tgtttccagt tcgatttcgc aggaaattga tttagaattt gtccctaagt ataatgttta
     6721 tattgatgta acgcgcgtcc gcatcctgca gaaggtcgac caattgggtg aaaaaaaatt
     6781 aaccgacatc ccaattagag taatcaatct tgctcaagat cagaaagccg aaattattcc
     6841 tccaacagcc acattgacta tttccggtgg aatcgaaaaa ttgaaaacag ttcagccgga
     6901 agatattaaa atcgtatttg attttgccac agattggcga ccggatgagt ccttttacac
     6961 tccaaccgta gaactgccac ccggactctt aaactggtgg aatctcgaac ccgatacttt
     7021 tgaaattcgg ataattcgcg agaggcaacc ctgaatgcta attctcggca tcgaaacatc
     7081 ctgcgacgaa accgccgttg ccattttaaa tgataatgaa attttagtca atctggtctc
     7141 ttctcaatta cttcatcagc agttcggagg cgttgtcccg gaatttgctt ctcgtgaaca
     7201 tatgcgtctg cttgatcaga tggtaaaaga agcattcgtc cagaccggat tgcagcccga
     7261 aaatcttgat ggtattgctg tgactcgcgg tcccgggtta atgggagctt tattggttgg
     7321 attaagtttc gcaaaaggtt tggcatattc acttcgtaaa cccttactgg gtgtcaacca
     7381 catcgaaggt catattatgg ctaatttttt gacctgcccg gatttaaaat ttccctttct
     7441 ctgtttactg gtttccggcg gccataccca aattatccag gtgaatgact ttggtaatta
     7501 tcagattctt gggcgcagtg ttgatgatgc cgccggtgag gcttttgaca aagccgcccg
     7561 cattctggaa ttaccttatc cgggaggtcc acaaattgat caaatggcac atcgtggtga
     7621 tcccaatttt taccaatttc cgcgggctaa agttcgctcc gagcagtacg atttcagttt
     7681 cagtggacta aaaaccgcga tgttatattt agttcgtcaa cgaggccaaa aatgggctca
     7741 ggaaaattta gccgatttat gcgccgctta tcaggaagcg atcgttgatg tcctgaaaat
     7801 caacactttc actgcagtca aaaatctgca cgccaataaa ctggtcttag ccggtggcgt
     7861 tgccgctaat tccagactaa gacaagtttt ccaggaacag gctgattact accaaatcga
     7921 attgataatt ccatctttga aatattgtac ggacaatgcc gcgatgatcg ccagaaccgg
     7981 gcaggaaatg ttgtcacgca accaaatttc gcctttttca atctcagccg taccgaatct
     8041 aacacttact actaaataaa atgcattcat tgaccttgaa aattccggga tttgaatggt
     8101 ggtggtttat ccttttattg ggattagttg gacttttatt cttctccttc aggaaaattc
     8161 ccggcggaag gtcgcggtca gtaacgacga tattgctggt tttacgcagt ttaattttga
     8221 ttctactggc aggattgatt cttaagccgg aaattcattg gacaagtagt cgcaaaaaag
     8281 cgccatcagc tttggtttgg attgacaatt cgcttagcat tgccgctcaa aataattttt
     8341 ctaaagacag tttgctttca attgtccagg aaattaatac agcactggat gggaaaggaa
     8401 tcaagccgca tctttttcta tttgatgaaa aaattattcc cgtaacggga aaactgaaaa
     8461 atatcgagaa taatggcctc gccaccgact tagcccaagt tttacgatat tctaagttcg
     8521 agtttgagaa cgaaaatatc gtaggtgcga taattatttc cgacggtgtc atcacccgag
     8581 gcgaagaccc aacttttagc gatatcgaaa tgccatttcc cgtgtttact attggaattg
     8641 gcgatagcga actggttatg gatcctgccg ttacgaaaat cgaaatgccc caaaccgtca
     8701 aagctggaga tacagttact atcaatgctg aaataatacc gactggaaac ggcgatccga
     8761 ttactgtaat gcttaaagaa ggtgaaataa ttcttgataa aaaagtcata gcgagtcaaa
     8821 gccaagctat gagaaaaatg ctgtcttttc aagcaattcc gtccaaaccg ggcgaaaaaa
     8881 tatattctgt tgaaattaca gccgcgcagg ataaaaatcc ctacaataat ctcaggatga
     8941 atgcggtgaa agtgctcgaa tcccaaatta aaatactcat tgtcagcggg caggctaatt
     9001 tcgaagcgcc ttttctgact cgcactctac gcggattgca agatgttttc gttcagaatg
     9061 tagttataaa taaaggacag tggttgccgc tctcatttca agagataatg gcaaaagagt
     9121 ggaatttgat aattcttatc ggctatccga cagccgaatc cagccagaac gatttaattc
     9181 gtctccggca aaaaatttcc gattacaatt tgccactaat attttttctc gatggagatg
     9241 tcgattctaa acagttagaa agactgctgg gctggaatcc gattgatgaa ctgttagctg
     9301 ataaatcagt aaatcagatt agcgtcaaac caacgcgcgc aggtctggaa catcctctga
     9361 taaaaaattt tcaaaaagta aatgtttccg aaataatctg gcgggaatta ccaccgattg
     9421 gctcgcctta ccgacggatt cgccttaagc cgtctttgct aacgattatt gaaagcactg
     9481 atatcgccga aaatccggtt attgccatca accaagaaag nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn gccgtagtca aagccgaact aattcaacaa aacgacgtga
     9661 tcgcctcaag caatttgatc tggaatggaa ataattattc gggagttata cccgttagaa
     9721 atgccggcga ttgtcaggta cgcatttcag cttggagtgg cgataattta atcggaattc
     9781 gcgaacagcc gatcacggta gttgatcgct cgatcgaatt acttgaggtt cggcaaaacg
     9841 tggatttact cagaacaatt gccttacgga gtggcggttt aaaaacgaac cggattcccg
     9901 aaattattga tcgcttacaa gtaacggaaa agattatcac ccagtcacac gttatcaagt
     9961 ttttaaactg gcgatggacg cttgtgattt tgattgtttt acttacagta gaatggagta
    10021 tgcgacgttt tcagggcttt ctataaaaag tcttgacctt ttaattgttt atgttcaaat
    10081 ttccaccgtg cagactagaa ttcaaaaaaa gaggtatttc agcaagaatt ggtaaatccg
    10141 atctggtctt agcacgtaaa acgcttaccc catttattaa atagaaaaaa ataccggagt
    10201 gattttatga agaaaattgc aatgctggga gtaggatatg tcggtttagt atccggaacg
    10261 ggactggctg atttcggtaa tgaagtaatc tgttatgata ttgatacgac taaaatagaa
    10321 aaactaaata acggagaaat gccaatttat gaaccgggtt tgattgattt ggttagaaag
    10381 aacgtcgcta aaggccgact gaagttcacc gacgatctga cgctggcaat acgggaatcg
    10441 gaagtgattt ttctggcggt accgactcct tccggcgaaa atggtgaagt tgatctttcc
    10501 tacattttta atgcggttga ccaaatcgcc aaggttattg acagttttaa agttattgtc
    10561 accaaaagta cagtcgaggt tggaactaat gatcaaatcc gcgaacgtct attatcacat
    10621 aaaattgcgc cggaaatgtt cgaagtggtt tcaaatcccg aatttttacg cgagggttcg
    10681 gcggttagcg attttatgcg gccggatcgg attgtgatcg gcaccagttc accaaaagac
    10741 ctcgagataa tgaaggaaat ttatcgtcct ctctatttaa tcgaaacgcc gattgtggca
    10801 accgatattc acaccgccga aatgattaag tatgcttcaa atgctttttt agcgacgaaa
    10861 atttcattta ttaacgaaat agctaatctc tgcgaactag taggcgccga tgttcagcaa
    10921 gtcgccaagg ctatgggtct ggacgggcgg atcggtaaga aatttctaca tgctggaccg
    10981 ggttacggcg gctcttgctt cccaaaagat actttagctt taactaaaat agctgaaaaa
    11041 gccggaatgc gatccacaat tgtggaagct gtcattgagg ccaataatcg tcagaaattg
    11101 cgggtcgtcg agaagctctt gaaagttcta ccaaacctct ccgataaaac gattggaatc
    11161 ctgggattag ccttcaagcc caacactaac gatgtccgcg aaacgccagc attaaccgta
    11221 attgaagaaa ttttgaaacg cggcgggcaa atcaaagcct atgatccgga agctatgaaa
    11281 gaaatgcgca aatattatcc ccaaattact tatgtggctt ccactgaaga tgccgctaaa
    11341 ggagccgatg ctttgattat tatgacggaa tggaacgaat ttcgcaattt ggacttaaat
    11401 tatctaaaat cagtgatgaa aaatccgatt atgattgata gtcgcaatat ttacgatccn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gttcgggtga ttaatctcga
    11581 taaacttaca tatgccggca acctggcaaa tctgcaagat ctggctgaca attccgatta
    11641 tatttttgtt aaaggcgata tttgtgatgc ggatactgta aataatattt ttcgtaaata
    11701 tcatccggat tacgtcgtga attttgcggc cgagtcgcat gtagatcgta gtatcggtaa
    11761 gccggacgat tttattcgga cggatatgtt tggcgttttt gttttattgg aagcttccaa
    11821 agaatttggg attgagcgtt tcatccagat tagtacagat gaagtctatg gctcaatttt
    11881 ggaaggttca tttaccgaag aatcgccatt gaaaccgggt aatccttatt cagccagcaa
    11941 agccggtggc gatcgtctgg cgtattccta tttcaatact tataacttgc cggtaattat
    12001 tacgcgcgct tcgaataatt acggtcctta ccaatacccg gaaaaactca ttccgctgtt
    12061 tgtcacaaat ttaatcgagg ataaacccgt tcccttgtat ggtgatggaa aaaatgtgcg
    12121 tgattggttg tacgtctggg atcattgtga tgcgattgaa ttcattctga aaaatggaaa
    12181 acccggcgaa gtttataata tcgccggaga aaacgagttg gaaaacatct tcatcaccaa
    12241 ctttattttt gaatatatga ataaaccgaa gacattgata aaaccagtcg cggaccgact
    12301 gggacatgac cggcgttact cgatttcggc ggctaaactg catgaactcg gctggcaagc
    12361 acaacacaaa ttcgaagatg ctatgcgcaa tactatccag tggtatattg acaatccgca
    12421 atggtggaaa ccattaaaaa gcggcgaata cctagaatat tataaaaggt attataaaat
    12481 ctaagaggta tattaatgaa aaaagcattt gttattctaa tggtggctat tttattcgat
    12541 atttcattag ggcaacaata tagatcctcc gaatctcaaa aatcgtatag atcctatggg
    12601 aaaatgtttg aacaagagac tgaaaaagaa aaaactactt taaaagaaaa agcaaaagaa
    12661 gtttcaaaaa tgcaagaact aattgctctt gataaagcag tttcacccga agaatatatc
    12721 gtaggccctg gtgatatatt tgggattgat ataattatca ctgaaaatat aaacatagaa
    12781 ttagctataa ctcctactgg ggatttatta ataccatcgg ttggaaaaat taatgtaaat
    12841 ggtttaattc tgaaagatgc catcgaacta tgtaaaaaga aaatcaatga agtctaccca
    12901 agtgccaaag tcgatgtagc attggtaaac ttacgcactt tcaagattca aattgccggc
    12961 gccgtgtttc aaccagggtt ctatgatgtt actgcggtta caagattaaa tgaaataatt
    13021 gagctggctg agggatttga ccaatttgcc aaggaattta atatccagat aactagaaac
    13081 gatggcagaa ttgacacaat aaattacttt aattataaac tcgagggaga cctgaagcat
    13141 aatcctactt ttttagaggg cgatagaata ttcgtcccat ttggagatcc ggataaagaa
    13201 gctatcgtta ttcgaggcgc agtgtatgga agtggctatg atattatcca agaaaatgaa
    13261 tccttaggct attatctacg acgccaagct cagtttattg aaaacgctga cttagaaaat
    13321 gtaacaatta cgcgaaaaga ggctgatcac cagctagtaa tgacggtata tccgcgagat
    13381 ttcttctcca ctccattaaa gccaggtgat gcaattgaca ttctctcaga aagaggaata
    13441 tcagttaatg gattcgtcca atctccaggt agtttttatt tctatccggg ctattcatat
    13501 ttggattata ttaatatggc tggtggaaac acctatgaag gagatatcga taaggtttac
    13561 atacgtcact tggatggaag taccgagcca ggtaaatctg caactcttaa acgaggtgat
    13621 gttataatta tccctcgtag ttctaagagt acaatattcg gagatttatc agttctggaa
    13681 atatttactt ccattgcaac tattttatta acttttattg ccgcaatgaa gtaaatgaaa
    13741 tttaaaattt tcataaataa caggagaatt acgtggcacc tataaaaaaa gaacatccgc
    13801 ttctctattt tatttttaaa catcgcgtca caaacctatt aattttactc cttggagcta
    13861 tcctagtttt tgtaatcgta ttttttggaa tgaagaaaaa atataccgct acagtgtcaa
    13921 ttttacctcc cagccaggat ttatcatcag gtataggcag ctcgatgggt gctttggcaa
    13981 atttagctgg gataaattta cctcagagta cactccagag tcctgagatg tataaagaaa
    14041 tccttacctc aagaaaagtt ttagatacaa tcctatttga agaatttgaa ggagttgatt
    14101 ccagcggtac aatttatcga aaaaagttaa ttgatatgct tgacatcaaa gccaaaaatg
    14161 aaagagaaaa aattgacaag gcattgaaaa aattactcaa agatgttatt tatgtaaata
    14221 tcaataatga aagtaatgtt actaagttat cggtgacact taccgatcct ttttatgcgg
    14281 ctaatgtagc caataggatg gtagaactat taaatgaaat tgtcgtaacc caaatccagc
    14341 atgaaaatcg tcaacaatta ctatacattg aagatcacat caaaaatacc caagacagta
    14401 ttaaaattgc agaaaaatat ttacaaacat ttttagagca ggtaaaatca attgctgagc
    14461 cagctaatca gatcaaagaa ttggcattaa aaagggatct ggaattgtat tctactatct
    14521 ggatcgaact taaacagcag aaagaaattt atacattaaa gtcaatccca agcctggcag
    14581 aagttaaagt ccttgatata gcccaagcac cttatttaaa gagtaggcct aaaagattac
    14641 taactatgat cagtctggga gggctctttc taattttaca aatatgtgta aatgctctca
    14701 ttttaattat taataatttt cgcaaagaac tcaagcctga tgaaccaatt ccccacccta
    14761 aatgagttaa agggattttt ctctaataaa gaaggtaaaa gtttatttgc taattttgct
    14821 caatttggca tatatcaggt tattatttat ttagcgccat taattactgt tccgtatctc
    14881 ttgaggtata ttggagtagc aaaatttggt atcatcagct ttgcaatggc tctcgtctat
    14941 tattttcaaa tagtcgtaga gtatggattt gggataacgg gaatccagat gattgcccag
    15001 agtcagaata atagtaggaa acaatcagaa atagtatcga atgttttaat aattcaaatt
    15061 atactattca gtatttgttt tatactatta gggatattat gttttttgat tccaaaaata
    15121 cacgattata gatccattta tctattttcc tacggctatg tcttagcgaa tatattttta
    15181 tttacctggt tttacattgg agtggaaaaa gtcaaatatg tcaacgtaat tatttctata
    15241 tcaaaattat tatatatact cttaattatc atttttatta aaaatgaaaa cgattttgtt
    15301 ttagtgccga tatttaattc ggggactacc tttctggcgg ggattgttag cattatcatt
    15361 ttaaggaaaa aattcatcat ccgtttcctc aagccaaata aggatatttt gaagagatat
    15421 ttaaaagaag gttggcacat ttttctctca aatattgcta tgaatttata tcgcaatact
    15481 aatgttatta ttctgggaat cttttcatca gatataagcg tgggaatata cagtgccggt
    15541 gaaaagatag tcaaagcaat ccaaagtacc ttatcacctc ttactcaaac cttgtatcct
    15601 tttatctccc gaaagaaaat tgaagaaccg gaaaaatcca taagatatat cagtagaatg
    15661 attacgatta tgggttttat tggatgtatt atctcaatat tactatttat ctttgcaggg
    15721 aaaattgctt atcttttaaa tgggtcagtt aatctggcaa tggtacgagt gattagaatc
    15781 gcctcttttg taattttatt cgggggtttg aattattgta ttggaataat ttttatgaca
    15841 aactacgata tgaagaaaga atttttaaag agcgtgatga ttgtgggaat attgaatact
    15901 ataatttgcg caattctttg tcatttaatc agcgaaattg gcgcggcaac tgctctattg
    15961 ctttccgaat ttattctatt tatgcttttg gtttaccata ttcaacataa taaatataaa
    16021 tgttcaacaa atcccttaga attatgagtt atccgcacgt atctatcata atcttgaact
    16081 ggaataaatg ggctgatacg attgagtgcc tggagtcggt attgaaatcg gattatccga
    16141 atttcaatat tattctggtt gataatggct cggataacga ttcagttgaa aaaatattag
    16201 catgggctga tggaaaatta ataaaacctt atattagtaa ttatgatgaa attgttttgc
    16261 cggaagtaaa gaagccttta attattcaga aaatacagat tgagaatatt aatcacgttc
    16321 cgactgaatc tggtaaaaac gctatgatta atctgttaat aagcaatgag aaccttggat
    16381 ttgctaaagc taataactta agcattagat atgctgacgc aacatggaat agtaaatatt
    16441 attatttatt aaataatgat acggtcatag agaaggaaac cctaagaaat cttgtaactg
    16501 tattagaaaa cgaaactcta tataaaatag ctcaatcgac catattttat tataacaata
    16561 aagaagaaat tgcttgtgcg ggcggtaggt tgtttccttg gggaaaaggt aaattttata
    16621 cccggattac gtcggacgag atcaaagaaa taggatttat aaacggatgc gctctattaa
    16681 tttcgcaaga aattgttgaa aaatatggtt tacttagcga aaggtttttc tttggtgaag
    16741 aagattttga gtatagctac agattgtctt tagcaaaagt aaaaaaagta tgtgtcggaa
    16801 atagtcgagt gtatcataaa attagcgtct cttctaatca cttactagat aatgatcgcg
    16861 gtcgtagctt agttttatca gcattaaaca gaatcgcaga tatgcgaaat tattatccca
    16921 gattcatctg gatattttgg cgctatctta ctgtcattta ttattattta ttcctcatga
    16981 ttattaaaca tggaatcgat actaaattag ccaataagta tttgaaatac attctgaaaa
    17041 aaagtagaaa tattagtagt gttgataaaa cattatacga agagataatt aataaaaaat
    17101 ggaactgact aaattatcta gtctcttttt tgcggctgtt atattagcta tgacgatttt
    17161 tgagaagcgt cgcttgaaca cgataattac acccttttcc gtatcagcgt ggcctttagt
    17221 gtttatcata ctcgtagtaa attttatgtt agtgaaaatc gaatttgata ttgtaacacc
    17281 acgagcaata ttattcttat tggcgaattt atttattata tggctaattg gcataatatt
    17341 ttcatatatt cataataacg ataggataca gtcactaaat gatatttttt taccgtatgt
    17401 taaatttgaa aaaatcctta tagttctttc ctgggtagta aatattgttg tatttttaaa
    17461 gatgaagaac cttttagcta aatatggcgg tttaagtttt ttaggagatt acagatttga
    17521 agaaatgata aacagtggac ctgaagcaca tatatcacaa tttggaaaag taacgtttct
    17581 actactgagt tttatttatc caatggcaaa tcataaaaaa actatcctta ttacactttt
    17641 tggatcaatt gttatgttat ttatgcttca gtcaaaatat aatcttattg gagttttagt
    17701 aatagtattt atctattata attttacaaa accagttaaa aagcagattg gcagtatttt
    17761 gaaattcagt ttattaacgt taatgataat ggctctttcc ttaatcacat tgaccatatt
    17821 ctggggtact tttaaagtat caaaacaatc agtatggcta ttcttatcaa aaaacttact
    17881 cagttatttt ttctcatctc cgattgtttt ggacaaatgg ttagaccatg cttttgtgca
    17941 acctgattgg acattactat tactatttaa aaatataata aaccgtatag tgggcaatcc
    18001 attagtatat agctcgttgg attacgtaga cttaaacttt tcagaagttg ccaaggatgt
    18061 ttattcaaac gttggaacaa gctatggaac gtattttatc attggaggtt ggatttttag
    18121 tttatttatg agtacagtaa tctcttcatt aacctatttt gtttattaca gcgccaagaa
    18181 aaaacctaat cctgtgaatg tatttttatg catgacttta ttaaacattg cgctttttag
    18241 tttttttggg caatatttta catttctacc atattatgaa atgattatat tttatatagt
    18301 taccataaag gtaatggata cctataactt atttattagt gattatcaaa attcgcttcc
    18361 attgcgaaag taatgtttcg gaaaattaaa atattacatg ttgtgaatgt atttggaggt
    18421 gctgttggcc gaatattggt tgacgtcatg ccacatttag taaaaaaatt tgatgttaaa
    18481 gtggtatcga tacaatcaat tgattattcg gaaccattgg tacaggaata tattaggaaa
    18541 tataacaatc tggaatcgct taaattaaat cgtttttctc tgtttaaagc atatcataag
    18601 ctaaattgct attttaaaaa gatagatcca gatttaattc attctcatat gggaagagct
    18661 gatattttta cagctatgtc aaaaactaaa aatatgataa taatgaatac cctccattgc
    18721 gaaaaaaaga accataatcc attgactcag atcggttata gactaactga tagaagggtc
    18781 gattggagaa tttgtatttc gcagactgtt gcgagaagtt attataatca tggttttaaa
    18841 agtccttatt cagtaatcta taatccaatt gatattaatg ggtttaccaa accaataaat
    18901 cgagaggaaa taagaaataa cctaggattt aagcctaacg agcaaattat aattaatgtc
    18961 ggtcggctaa ttaaatgtaa aggtcagagg taccttatag aagtaatgcc gcatttatgt
    19021 catttattac cggaaataaa acttctcatt gtgggagatg gccctcagta taatagtttg
    19081 aaaaaattga ttattaataa taacttagaa aataatgtat atttattagg ctaccggcaa
    19141 gatgtacaaa atctgttaaa aatatccgat ctgtttgttt attcagctca gtgggaaggc
    19201 ttaggtatgg cagtaataga agcaatggcg actagaattc cagttctcgc tcaccccctg
    19261 cctgccctca aagaatatat cattgataat cacaatggtt atttttgcaa tattcactta
    19321 aaagaatcgt tcgccaataa aatttatgat atacttacta attctcatca taattcatca
    19381 atagctgaaa atgctttcag aacggtgact gaaatgtttt ctgctgataa aattagtaaa
    19441 caatacattg aactttatca gcaattacta tcaaatagat aattaatagg actatagatt
    19501 aaggataaga gtataaagac agtcatttta gtttcaaata ccagttggta tttatataa
//
LOCUS       DAOMJU010000036        19328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2667_length_19328_cov_24.518445, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000036 DAOMJU010000000
VERSION     DAOMJU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 19328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19328
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2667_length_19328_cov_24.518445"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..765
                     /locus_tag="PLO58_05040"
     CDS             52..765
                     /locus_tag="PLO58_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQO74189.1"
                     /translation="MQFLVLYDNSSLSPAIRCGWGFACLVDKRILFDTGDKYEPLCEN
                     LAQLKINPKTIEAVVISHDHHDHTGGLWGLLERQPGLKVYACPGFGNDFKQRVNALRG
                     KLIESIGFRKIDDHIGVTGEIPAQYKGSPKPEQALIARTARGIAVVTGCSHPGIITIL
                     TKVKTIFPAEKIALILGGFHLRDSACEAIEEIASQLVTVGVQKVSPAHCTGEPAQAIF
                     KRRFGANYVPAGAGNQIEI"
     gene            806..1126
                     /locus_tag="PLO58_05045"
     CDS             806..1126
                     /locus_tag="PLO58_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HQO74190.1"
                     /translation="MKLGMIITQTDAETVFNGLRLALYSLEQGDEVRIFLSGKGVEID
                     QIEDSKFDVKTLAQKVIDKGGQFLACGACLKLRNSDGSEVCPLSTLKDQYQIVRDSDK
                     IVTI"
     gene            1138..2019
                     /locus_tag="PLO58_05050"
     CDS             1138..2019
                     /locus_tag="PLO58_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQO74191.1"
                     /translation="MQELVIISGKGGTGKTSIAAALAVLAKSAVVADCDVDAADLHLV
                     LSPQLKERHDFFAGHEAVIRQADCIACGDCLAHCRFEAILTRQEASAGNKFFVDPIAC
                     EGCGVCVQFCPVKAIDFPDRLCGEWFVSATRVGPLVHAKLSIAAENSGKLVSTVRQSA
                     RRLAESEKRALVIVDGPPGIGCPVIASITGATQVLVVTEPTVTGEHDLERVLALTRYF
                     NIPTSVCVNKWDLNPGMTEQIEKKARKSSAKTVGRIRYDLSVTKAQLQALSVVETDSP
                     CAMDIKNVWNKLGLTGD"
     gene            2023..2931
                     /locus_tag="PLO58_05055"
     CDS             2023..2931
                     /locus_tag="PLO58_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HQO74192.1"
                     /translation="MSDISKEKTKNTCDSCGNSTCSAAKPREGENQQEFKERQQLLSR
                     LCHIRHKIIVLSGKGGVGKSTVAVNLAVALMLAGKRVGLLDVDIHGPSIPTMLGLEDE
                     VIQKDDDGMIPIELGDLKVMSIGFMLRNPDDAIIWRGPLKMGIIKQFLKDVSWGELDF
                     LIIDSPPGTGDEPLSVCQLIGDLDGAIVVTTPQKLAAVDVRKSITFCRHLNVKVLGIV
                     ENMSSFVCPKCGEVTPIFGTGGGKKIAESMQVPFLGSIPIDPKIGQTGDSGQVFIHQY
                     SKSPTAEIMRQIIKPILDMDSENVKS"
     gene            2950..3288
                     /locus_tag="PLO58_05060"
     CDS             2950..3288
                     /locus_tag="PLO58_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HQO74193.1"
                     /translation="MKIAIPLANGKLTLHFGHCEQFALVDVEVQNKKIIKRTDLIPPP
                     HEPGLLPSWLAEHGVNLIIAGGMGQRALMLFAENNIQVLVGACSESPEKLIGDYFDGN
                     LQLTSNVCDH"
     gene            3422..3832
                     /locus_tag="PLO58_05065"
     CDS             3422..3832
                     /locus_tag="PLO58_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQO74194.1"
                     /translation="MRSWTLPALIALILWGFWGFLPKVATRYIDPSSALVYQAIGGTI
                     VGLGVLLSVRSQIQWQAVGAFWAILTGLFGMLGSLFFLQAVSRGKVTTIVTMTALYPL
                     ISIVLALIFLKEPFSFRQGLGVFCALMALILFSI"
     gene            3846..4619
                     /locus_tag="PLO58_05070"
     CDS             3846..4619
                     /locus_tag="PLO58_05070"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020029636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HQO74195.1"
                     /translation="MKEKIEIARSWLPRYTGLPLEKYGDYILLTNFRNYVDIFCAMFK
                     CDLYGENRPMQAATNSAGLTIINSGIGSPNAATIMDLLVAVHPKGVLFLGKCGGLKKS
                     TEIGHFILPIAAIRGEGTSNDYFPPQVPALPSFKLHKFVSEIIVQRGLEYRTGVVYTT
                     NRRVWEHDQAFHQRLREMTCIAIDMETATLFIVGHYNEIARGALLLVSDLPTTPEGIK
                     TEELDKQVTAQWSQVHLQIGIDAMTNIGRMGEPIKHFRY"
     gene            4649..6328
                     /locus_tag="PLO58_05075"
     CDS             4649..6328
                     /locus_tag="PLO58_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74196.1"
                     /translation="MRNIRFVNLTFIIIIAFPLVVSAIGIDVPATDPVYDFLKRLETR
                     GVIKGFNDLTLPLTRDEIVNFLQIASQKADELSAIEKKILHEFLADYRYETERQLTEP
                     DSISAPFYQKNYFRQATANLFSRQDAVEENHLLMYESGEAFFWFDVGARGRMDWKENH
                     QKQMFSDRYLLRGALGKSFTFHTYFARFMKNGNPAFTEPYEEEIGNFSMVQPGGTVTF
                     DILQTSLVFHQKYFNIGLYRQPVAWGASGTNNLILSKSAPLFSYIGFDTKFKGIKLSM
                     IHGSLLNDSTHYRDIAFQQRNRSKYIAAHRLDIPFFRGTTTVGFSEMVIYGDREIDIA
                     YMTPFGFYWPIGHALEDRDNTLMAMDVKTTFIRNITFYGSVLIDEMRFSELGKQWWAN
                     KHAWQAGMRWSPNIATLPFDFQAEFTAVRPWTYSHKTLTTNYTHNSICLGFPYGANSQ
                     TWYFKIGTALSRRLTLTSEYLILKKGVDDSTHFWGGDATVSYELRDEQYDHATKWLMG
                     DIETTNQLKVAGRYEIFNDFFIKAGINLYNTKLNNDQESYRFGFIETEINF"
     gene            6328..7422
                     /locus_tag="PLO58_05080"
     CDS             6328..7422
                     /locus_tag="PLO58_05080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO74197.1"
                     /translation="MLNLIEFAILLTFFYLIIMGVIGIGLIFIRTQKRVFQTLAVSVI
                     VCARNEGQIISRLLQSLTNLRYPEDKYEIILVDDDSTDDTLNIMNDFASGKSNWKVLH
                     HDKTTDQLKGKKGAITLGVQNSCGEIILVTDADCVVPPNWIASMVSYFRKEVGMVLGH
                     SPVARRKGFMNIYERFDTLCEASVAAASTFYNKPTHANGRNLAYRKKAFEDVNGYYRS
                     ANTDTGDDFFLMQEIRTKTRWRFAYNIDPASYVTTEPTKFGKKYIHQQLRRNSKAFNL
                     TIPFFILGVIVFLFHVVLAIMLFSPSLWNWLGMMLFFKFMAEFIAVYHGAHLFHQTDL
                     LKYFPILWVIYPVCYLLSQVIGSLKIYRWK"
     gene            complement(7434..8570)
                     /locus_tag="PLO58_05085"
     CDS             complement(7434..8570)
                     /locus_tag="PLO58_05085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO74198.1"
                     /translation="MIIIHLITFISIVIYLVSLIIFRWGLNHPRQQRTADLPTISIVI
                     AVKNEAVNLPRLIEQLTGQNYPDGKLEIIIVDNDSTDETWAILQNEAARISNLRIFTT
                     RTEGTPYRHKKAALAVGIREARGEVILTTDADCSMGKDWARTMASYFTDDVGIVVGFS
                     AIRYLNDWFSRLQALDYLQLMAAAQGSTNLGLAWACSGQNWAFRKVLFENAGGYMLVR
                     DRVGGDDSLFMQVMQKRTRTKVVFAADKAAWVETQAADSLTTFLRQRIRWASEANYMH
                     HFNCLFFVVILATFLANLMPIVYFVLAITGVVTFVPFLIVIAIKLIGEAMLAFKAIRV
                     YQRKDLMGVFIPWFIIQPFYIVLMGILSFFGNRLGWGEQETRSA"
     gene            complement(8589..9488)
                     /locus_tag="PLO58_05090"
     CDS             complement(8589..9488)
                     /locus_tag="PLO58_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HQO74199.1"
                     /translation="MINNHQYFEPELKPHPPIIVPKIKTAIGLLIMGLICATLVSLMI
                     MIFVPTSNLTKTTQLLLLIAELFLPVPIFFWAWRRHTNLGELLRIKRVSSPSIIASMI
                     VSVSLTVIIDELDRLIQIVLPLPESFASIGEIMLITDWQSALLIIGVVVFAAPLVEEM
                     MFRGFFQRILEYRQGDITKSVLLSAMVFALVHFNPWWVVQIYLMGLFLGYLAWRANSI
                     WPSFVLHAVNNGWSVWWSHQPAFAKFYEWHGHVHPVLIIASLVCFYLGMRFFIKVTPV
                     VEKRQDIVLIENLSDYYENEPPE"
     gene            complement(9500..10045)
                     /locus_tag="PLO58_05095"
     CDS             complement(9500..10045)
                     /locus_tag="PLO58_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQO74200.1"
                     /translation="MAFLGYVLAIILLSVTPASPQVTVDTLAIGTVAPSFYASVLDGS
                     DFFLSHKVGAKAHPNEKAPVILVFFTTFCIPCRQEIPFLDSLRIEYPQIAFYLVNIGE
                     EPQIVKEYIQRMNYKMPVLLDRYGQVAKRYQASITPTLVGINVNGQIAFLKRGFQVSD
                     KELIQTNIKKLTDDNLFIEKN"
     gene            complement(10057..10824)
                     /locus_tag="PLO58_05100"
     CDS             complement(10057..10824)
                     /locus_tag="PLO58_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQO74201.1"
                     /translation="MILTRNERALYRSLKQKKERERTGLFVIDGIKPVQEALIATFPL
                     ERILISESIDEAERLSLKSLADKQGIAIIVVTQSEIEQISALRNPEGVLAIAKIPSEV
                     NLLAPLDYPALYLWQINDPGNLGTILRTALWFDVKTILLSPDSVDVYSPKVVRGAMGA
                     LFRLKVRTEVDLSRIEMILGRDNAQMWAADMSGTIEKPEITNDRFVIAFGSESHGLPP
                     EILQRATGLIRINKYGYGESLNVAVAVGIILNNIRQK"
     gene            complement(10827..12902)
                     /locus_tag="PLO58_05105"
     CDS             complement(10827..12902)
                     /locus_tag="PLO58_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HQO74202.1"
                     /translation="MSAKVKSLTKRISSFFFSLRMQIANNPLNEYIRLLFFAFLIGVL
                     AGLVTLAFYKLIHLISYFLYDYLPARYNNLPKWTMALFPAVGGLIVGLMIKLWPRQPR
                     QRGVVEVIKASFIGRGVIRVTTIIFNFIATAIFLGTGGSLGPEAPVAFLGAGSGSLIG
                     QAFRVSENQRRVFLAAGTAGAIAAVFNAPIGGVFFAIEIVLFNNFQTLTFSALIISAI
                     TADYISRVFLGDQLLLTLPKVEMALSLNILYFAVLGIITGLFSLAFIRFSGKMHNFFD
                     AQKGRFAPLLRLTGLMLILGIVGLFFPQIIGIGYDAIREIANNNYTWQVLAIVFVLKF
                     LFSGLLSAAGGFGGLFAPSLLMGAALGAFLAKGGNQVLGLSLDVAGFAIAGMGGMLAA
                     VNSIPVTALLIIVEITGNYHLVLPLMLTVIMSYIIVHYVLKETVYSIELRQRGIQLFD
                     GKELNVLKNVKIDQIMSYEFHKVYSTMDVKTLVKVIAEGAANIYWVENEQHKIIGFIS
                     WSDLKPLITDYQNLNTLVLVSDVMNPNVVSVKTDDTLDYVMKLFGRYNVDELPVTDPA
                     TGMLIGTVNRSRVIQLYNQEIFKRETAGDLATHIRYLDQTRFVEIDEDFSILEMSAPE
                     SMVNSTLKALDLRNRYHAQIILIKRLDNKGRRMVITPRADTIILPNDILILAGPTKKL
                     YELKERYYL"
     gene            complement(13229..14407)
                     /locus_tag="PLO58_05110"
     CDS             complement(13229..14407)
                     /locus_tag="PLO58_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQO74203.1"
                     /translation="MFKEKIRQAIEILKEQGVDMWMTFVRETETLPDPCMDLIVGTNC
                     TWQSAFIITAKGQTIAIVGSLDKERIKQTGLYDDIRIYVDDFGAAFLNALNEINPNRI
                     AINTSINDYMADGLTHGMYELLMGYTKNTPYRERFISSEKIVAALRGRKSPTEIQRIK
                     RAIEITEKIYAEISPQIKSGRTEKEIADIILKMVAELGVQTAWESNSCPAVFTGPLSA
                     GAHAEPTERVIQPGHVLNIDFGVKKDGYCSDIQRTWYILRNNEETAPEPVQKGFAVLL
                     ESVRRAVDAIRPGKMGYEIDQIARDYITANGYPEFPHGLGHQVGRSTHDGAGMLGPRW
                     ERYGDTVYLPLEIGQVFTIEPRLPIEGFGVATVEEMIVVTADGCEYLSHPQNQLFYSR
                     "
     assembly_gap    14433..14532
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14804..17620)
                     /gene="uvrA"
                     /locus_tag="PLO58_05115"
     CDS             complement(14804..17620)
                     /gene="uvrA"
                     /locus_tag="PLO58_05115"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQO74204.1"
                     /translation="MSTEHIVIKGAREHNLKNIDVKIPRDKFVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGLMEKPDVDFIEGLSPAISIEQKSASRNPRSTVGT
                     VTEIYDYLRLLYARIGIPYCYQCGKRIERQTTQQIVDNILSWPEDTRLMILAPLVRGR
                     KGEYREVFREVRREGFLRVRIDGKIYEVEHTPVLDKNKKHNIEVVIDRLSLNPTVKDR
                     LTESVELALKIGSGLLVVVINSENEQIFSEKLACTDCGISYEELEPRMFSFNSPYGAC
                     PTCDGLGTLMEIDPALVVPDETKTLSQGAIVPLGEQPRGGWYSAILKGLARKYNFDFV
                     TPFKKLPQEAKNALLYGSDGTKLSINLKSDRIDGEYHTPFEGVIPNLRRRYKQTTSTG
                     IRMWIQQFMSYRLCPQCQGARLKPESLAIKIGGFAINQLSAMSITKLREFFVNLELNP
                     TEQQIAHQILKEIHTRLNFLINVGVEYLTLDRMTHTLSGGEAQRIRLATQIGSQLMGV
                     LYILDEPSIGLHQRDNRRLIETLLHLRDNGNTVIVVEHDREMIESADYIIDLGPGAGA
                     TGGNVVFAGTPAEIKNEPRSLTGQYLSGAKTIPIPRNRRPGNAKQISLYGATGNNLKN
                     IDVCFPLNKFICITGVSGSGKSTLINETLFPILHRNFYGSQKVPLRYTDVQGLEYIDK
                     VIAIDQSPIGRTPRSNPATYTGTFTHIRDLFANLKESKIRGYTAGRFSFNVRGGRCEV
                     CGGDGIKKIEMHFLPDVYVLCEECKGLRYNRDTLEVKYKGKSIADVLNMSISEAFEFF
                     RNIPSINKKLQTLIDVGLGYIKLGQQATTLSGGEAQRVKLSTELSKVGTGKTLYILDE
                     PTTGLHFDDIKMLLNVLNRLVDQGNTVIVIEHNLDVIKTADYIIDLGPEGGDNGGYIV
                     CEGTPEEVSAHPASQTGKFLAKIL"
     gene            17806..>19328
                     /locus_tag="PLO58_05120"
     CDS             17806..>19328
                     /locus_tag="PLO58_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQO74205.1"
                     /translation="MTQTGLDQETRTMILDTINSLKNELLTREKILELDDKNEFPLEI
                     VKELLSDRIGLQMVFIPEEFGGLGGGALDTYYVSVELAKICLGVATAFLAIHLGAEPI
                     LLFGTEEQKQKWLAKIVNEAAIVAYAVTEADAGSNLDAIKTKAEPMTDESGNIVSYRL
                     NGSKQFISNGGYADFLTLLAKAPEGATFFIVDGKSTGLKRGKPEHKHGIRAANTSPLT
                     LENVIVPAENIVGGVPGVGLKQANDVFSYTRLMVAAFGLGAGLSAMEKAIQFAKTRIQ
                     FGTPLIEKQGYTHKLLVPNVVRLTAAQAYIEEIAKRVDSGDTDILVEGSIAKYFATEA
                     GNIAAEAAIQALGGYGYMHDYEVEKIKRDVRITMIYEGTSEIQQNIIYLYRWRTTVKS
                     KGEFYQKMAQEMRTIAEQSNLESAKIVAQALELLNDLIMFAHNQKLTRQQHIQFLLSD
                     VITWSEVAAALVRKTAEHLRDALPDAIQLQSVLRIFVAEMARLISNKGMEIILGSETV
                     "
BASE COUNT         5384 a         4216 c         4307 g         5321 t
ORIGIN      
        1 aatctttaaa cgatgagatc cgcaagtgaa caaggttgcc ggagacggaa aatgcaattc
       61 ttagtgcttt atgataattc atcgttgagc ccggcaatcc gctgtggctg gggatttgcc
      121 tgtctggtag ataaacgtat tctcttcgat accggcgata agtacgagcc gctttgtgaa
      181 aatttggcac agttaaaaat aaatcctaag acaatagaag ctgtcgtgat ttcacatgac
      241 caccacgatc ataccggcgg attatgggga ttattagaac gacagccggg cttaaaagta
      301 tatgcctgtc ccggatttgg caatgatttt aagcaaaggg taaacgcttt acggggtaag
      361 ctgatcgaat cgatcggttt caggaaaatt gacgatcaca ttggtgtaac gggtgaaatt
      421 cccgcccaat ataagggttc accgaagccg gagcaagcct tgattgcccg aacggcgcgc
      481 gggatcgcag tggtaaccgg ctgttctcat ccgggaatca ttacaattct tacgaaagtg
      541 aagacaattt ttcccgctga aaaaattgca ttaatcctgg gcggatttca tttgcgagat
      601 agcgcatgcg aggcgattga agaaattgct tctcaattgg tcacagtagg agtgcagaaa
      661 gttagtccag cccattgtac cggcgagcct gcccaggcaa tttttaaaag aagattcggt
      721 gcgaattatg ttccagccgg cgccggcaat caaatagaaa tataaatgag agcaaaaaag
      781 tgtaaatgaa aggctgaaat gcaaaatgaa actcggaatg attattactc agactgacgc
      841 cgagaccgtt tttaacggat tgcgtctggc actctatagc cttgaacagg gtgacgaagt
      901 gcgcattttc ctttccggta aaggcgtcga aatcgatcaa attgaagatt caaaattcga
      961 tgtcaaaacg ctggctcaaa aggtaattga caagggcggt cagttcctgg cttgcggagc
     1021 ctgtctaaaa ctacgcaact ccgatggttc cgaagtctgt ccgctttcca ctctaaaaga
     1081 tcaatatcag atcgtccgcg attcggataa aattgttact atctgaggaa tccggcattg
     1141 caggaattag ttatcatcag tggaaaaggc ggtacaggta aaaccagcat cgcggcggca
     1201 ctggcggttt tagccaaaag tgcggtagta gccgattgcg atgtggacgc cgccgactta
     1261 cacctggttc tttcgccgca gttgaaagaa cgccatgatt tttttgccgg acatgaagcc
     1321 gttatccgtc aagccgattg tattgcctgt ggcgactgcc tggcgcattg tcgctttgaa
     1381 gcaatcctga cccgccaaga agcgtcagcc ggaaataaat tcttcgtcga tccgatcgcc
     1441 tgcgaaggtt gcggcgtctg tgtgcaattt tgcccggtga aggcgatcga ctttccggat
     1501 cgtctctgcg gcgaatggtt tgtttcggca acgcgcgttg gtccgttagt gcacgccaag
     1561 ctcagtatcg cggctgaaaa ctccggcaaa ttggtctcaa cggtacgcca atctgcccgc
     1621 cgacttgccg aatctgaaaa gcgcgcttta gttattgttg acggaccacc aggaatcggt
     1681 tgtccggtca tcgcttcgat tactggcgcg acccaggtgc tcgtggtgac agaaccgaca
     1741 gttaccggcg aacacgatct ggaacgcgtg ctggctctga cgcgctactt caatattcca
     1801 acttcagtat gtgtcaacaa gtgggatctt aatcctggaa tgactgagca aattgaaaaa
     1861 aaagcccgaa agagttccgc caaaactgtc ggtcggatac gttatgattt gagtgtgacg
     1921 aaagcgcaat tacaagcctt atcagtcgta gaaactgatt cgccctgcgc aatggatata
     1981 aagaatgtct ggaataaatt aggtcttaca ggagattgaa atatgagtga tattagtaaa
     2041 gaaaaaacaa aaaatacctg cgattcctgt ggtaattcga cctgttcggc ggcaaagcct
     2101 cgggaaggtg aaaatcagca ggaattcaaa gagcgccagc aattactttc ccggctttgt
     2161 cacatccggc acaagattat cgtgttgtcc ggcaaaggcg gcgtcggtaa aagcacggtg
     2221 gcagttaatc tggccgtggc tctgatgctg gcaggtaaga gagtcggttt gctggatgtg
     2281 gatattcacg gaccaagcat tccgactatg ctggggctgg aagatgaagt aattcagaaa
     2341 gatgatgacg ggatgatacc gattgagctg ggcgacctaa aagttatgtc gattggcttc
     2401 atgcttagaa atccggatga tgccattatc tggcgcggac ctttgaaaat gggtattatt
     2461 aagcaatttt taaaggatgt cagctggggc gagcttgatt ttctgattat tgactcgccg
     2521 cccggaaccg gcgacgaacc gctttcggtt tgtcaactaa tcggcgacct ggatggcgca
     2581 attgtcgtca ctactccgca aaaactcgcc gcagtggatg tgcgcaaatc aattacattt
     2641 tgtcgtcatt tgaatgtcaa agtactcggt attgtggaaa atatgagcag tttcgtttgt
     2701 ccgaaatgcg gcgaagtcac gccgattttc ggtaccggtg gcggaaagaa gattgccgaa
     2761 agtatgcagg ttccctttct gggttctatt ccgattgatc cgaaaattgg ccaaaccggc
     2821 gacagcggtc aggtctttat tcaccaatat agtaaaagcc caactgcgga aatcatgcgg
     2881 caaatcatca agccaattct ggatatggat tccgaaaatg ttaagtctta atcacaggag
     2941 aataaaaaaa tgaaaatagc cattcctctc gccaacggca aactaaccct gcacttcgga
     3001 cattgcgaac aatttgcgtt ggttgatgtt gaagttcaga ataagaaaat aatcaaacgc
     3061 accgatctta taccgccgcc gcacgagccg ggtttactac ctagctggct ggcggagcac
     3121 ggcgtaaatc taattatcgc tggcggtatg ggacagcgcg cgctgatgct tttcgccgaa
     3181 aataatattc aggtgctcgt gggcgcctgt tctgaatcac cggaaaagtt aatcggtgat
     3241 tatttcgatg gaaatctgca actgaccagc aatgtctgtg atcattgatg tttttaatca
     3301 ctatccacat atatcttcat atctataatt agattagata aatttgactg gccagaagaa
     3361 tttttgtact ttgctcagca aatttgatag tttaatcgaa ttattcacaa ggagaattga
     3421 cttgaggagt tggactttac cggcactaat tgctttaatc ttatggggat tttggggctt
     3481 tttaccgaag gtagccactc gctacattga tccgtcgagc gcactcgtct atcaagccat
     3541 tggtgggaca atcgttggtt tgggggtttt actatcggtt aggagccaaa ttcaatggca
     3601 ggcagttggc gcgttctggg caattttaac cggtctgttt ggtatgctgg gttcgctgtt
     3661 ttttctccag gctgtcagtc gtggcaaggt tactaccatt gtgacgatga cggctctcta
     3721 cccattgatt agtattgttc tggcgttaat ctttttaaaa gaaccgttta gtttcagaca
     3781 gggtttaggt gtgttctgtg ctttaatggc tctgatttta ttttcaattt gaagaaggtg
     3841 tagttatgaa ggaaaaaatc gaaattgctc ggagctggtt gccacgttat actggtctgc
     3901 ccctcgaaaa atacggcgat tatattctgc tgaccaattt ccgcaattat gtggacattt
     3961 tttgtgcgat gttcaaatgt gatttgtacg gtgaaaatcg tccaatgcaa gccgccacga
     4021 attcagctgg tttgactatt atcaattccg gaatcggttc gccgaatgcc gccactatta
     4081 tggatttgct ggtggcggtt catcccaaag gagtgctgtt tttaggaaag tgcggcggct
     4141 tgaagaaatc aactgaaatt ggacatttta ttttgccaat cgccgctatt cgcggagagg
     4201 gtaccagcaa cgactatttt ccaccccaag ttccggcttt gccatcgttc aaattgcata
     4261 aatttgtctc agaaattata gtccagcgcg ggcttgagta ccgcaccggt gtagtttata
     4321 caaccaaccg tcgagtttgg gaacatgatc aggcgttcca ccaacgctta cgcgaaatga
     4381 cttgtatcgc tattgatatg gaaaccgcca ccctttttat cgtcggacat tataacgaga
     4441 tcgcgcgcgg cgctttactc ctcgtttcag acctaccaac gactcccgaa ggtattaaaa
     4501 ctgaagagtt agataagcaa gttacggcgc aatggagtca ggttcactta caaatcggta
     4561 tagacgctat gactaatatt ggaaggatgg gcgaacccat taagcatttt cgctactgat
     4621 aaaataattg tatatttaag taccaatgat gagaaacatt cggtttgtaa acctaacttt
     4681 tattatcatt atcgcttttc cgctggtagt ttctgctatt ggtattgacg tcccagctac
     4741 cgatccggtt tatgattttt tgaaacgcct cgaaacgcgg ggcgtcatca agggatttaa
     4801 cgatctgact ttaccgttaa cccgtgatga aatcgttaat ttcctgcaaa tcgcctccca
     4861 aaaagctgat gaactttcag ccatcgaaaa gaaaatttta cacgaatttc ttgccgatta
     4921 tcgctatgaa accgaacgcc aattgaccga accggattcg atctcagccc cattttatca
     4981 aaaaaactat tttcggcagg caacggccaa tctgttttcg cgacaagatg cggtagagga
     5041 aaatcatcta ttgatgtatg aaagtggcga ggccttcttc tggtttgacg tcggagcgcg
     5101 tggtcggatg gattggaaag aaaatcacca gaagcaaatg ttttccgatc gctatctatt
     5161 acgaggggcg ctggggaagt cgttcacttt tcacacctac tttgcccgct ttatgaaaaa
     5221 tggtaatccg gcgtttacgg aaccctatga ggaggaaatc ggcaacttca gtatggtaca
     5281 acccggcggg acggtcacct ttgatatttt gcagacttct cttgtttttc atcaaaaata
     5341 cttcaatatc ggcttatacc gtcagccggt tgcctgggga gcaagtggga ctaacaactt
     5401 gattttatca aaaagtgcgc cgctgttttc ttatattggg ttcgatacca aattcaaagg
     5461 gataaagctg tctatgattc atggatcgct attgaacgac agcacccatt atcgcgatat
     5521 tgcatttcag cagagaaatc gttccaagta tatcgccgcc catcgtctcg acattccttt
     5581 tttccgcgga acgactactg tcgggtttag cgagatggtg atttatggtg atcgagaaat
     5641 tgatatagcc tatatgacac cttttggatt ttattggccg atcgggcacg ctctcgaaga
     5701 ccgcgacaat actctaatgg cgatggatgt taaaacgacc tttatcagaa atatcacgtt
     5761 ctatggctcg gttctaattg acgaaatgcg ttttagtgaa cttggtaaac aatggtgggc
     5821 taataagcac gcttggcagg cgggcatgcg ctggtcgcct aacattgcca ccttgccttt
     5881 tgattttcag gcagaattta ccgccgtgcg cccctggact tattcccata agactctgac
     5941 aaccaattat actcataata gcatttgcct tggatttcca tacggcgcca attcccaaac
     6001 ctggtatttt aaaattggga cagcgctttc aaggcgctta accttgacca gcgaatacct
     6061 gatcctgaaa aagggagttg acgattccac tcatttttgg ggcggagacg ccactgtcag
     6121 ttatgagcta cgtgacgagc aatatgatca cgccaccaag tggctgatgg gtgatattga
     6181 gacaaccaat cagctcaagg tcgccggccg ctatgagata tttaacgact ttttcattaa
     6241 agccggaatt aatttgtata atactaaact taataacgac caagaatcct accgctttgg
     6301 atttattgaa actgaaatca atttctaatg cttaatctaa ttgaatttgc gatcctgctg
     6361 acattttttt atctaattat aatgggagta atcggaattg gcttgatatt tattcgcacc
     6421 cagaaacgag tgtttcagac acttgcggtt tcagtcattg tctgcgcccg caatgaagga
     6481 caaattatta gccggttatt gcaaagcctg accaatttgc gctatcccga agacaaatac
     6541 gaaataattc tggttgatga tgattctacc gacgacactc tcaacattat gaacgatttt
     6601 gcttccggaa aatccaattg gaaggttctc catcatgata aaactaccga tcaactcaaa
     6661 ggtaaaaaag gcgctatcac gctgggtgtt cagaatagtt gtggcgaaat tattttagta
     6721 accgatgctg attgcgtagt tccgccgaat tggattgctt cgatggtatc atatttccgc
     6781 aaagaagttg gcatggttct cggtcatagc ccggtcgcaa gacgcaaagg gtttatgaac
     6841 atttatgaac ggttcgatac cctttgtgaa gcgtccgttg ccgccgcttc gactttttat
     6901 aataaaccga cgcatgccaa cggacgtaat ctggcttacc ggaaaaaggc ttttgaagat
     6961 gtcaatggct attatcgaag cgctaatacc gatacaggcg atgatttttt tctaatgcag
     7021 gaaatccgca cgaaaacccg ctggcggttt gcttataaca ttgacccagc cagttatgtt
     7081 accacggagc caacgaagtt tggtaaaaaa tatatccatc aacagttgcg ccggaatagt
     7141 aaagcattta atctgactat tccctttttt attctcggag taattgtttt cctgtttcat
     7201 gtcgtgctgg caataatgct tttttcgccg tcactttgga attggttggg aatgatgttg
     7261 tttttcaaat ttatggctga atttatcgcc gtttatcacg gcgcccactt atttcaccaa
     7321 acggatttgc tcaaatattt tccgattcta tgggtaattt acccggtctg ctatttgtta
     7381 tcgcaggtga tcggctcatt gaaaatctat cgctggaaat aaatttttga tcttcaggca
     7441 gaccgagttt cctgctcgcc ccaacccaaa cggtttccaa agaagcttaa aatacccatt
     7501 aaaacgatat aaaatggttg gatgataaac cagggtatga aaacacccat caagtctttt
     7561 ctttgataaa cccggatcgc tttaaaagct aacattgctt cgccgattaa ttttattgca
     7621 attacaatta agaacggaac gaaggtaact actcccgtta ttgcaagaac aaaatagaca
     7681 atcggcatta gattagccag aaatgtggct agaattacga cgaaaaacag gcaattgaaa
     7741 tggtgcatgt aattagcttc gcttgcccag cgaatgcgtt gacgtagaaa agtcgtaagg
     7801 ctatcagcgg cttgggtttc cacccaggcg gctttatccg cggcgaatac tactttcgtg
     7861 cgagtgcgtt tctgcataac ctgcataaaa agtgagtcgt cgccgccaac tcgatcgcgg
     7921 accaacatat agccgccagc attctcaaat agtactttac gaaaagccca gttttgacca
     7981 ctacaagccc aagccaagcc gagattggtc gagccttggg cggcggccat caattgcaga
     8041 taatccaaag cctgaaggcg cgaaaaccag tcattgagat accgaatagc cgaaaatccg
     8101 acgacgattc cgacatcatc agtaaaataa ctcgccatcg ttcgcgccca gtcttttccc
     8161 atggaacagt ctgcatccgt agtcaggatt acctcgccgc gggcttcgcg aattcctacc
     8221 gctaatgcgg cttttttatg acgatacggt gttccttcgg tcctggtagt aaagatccgc
     8281 aggttactaa ttctagcggc ttcgttttgt aaaattgccc aagtctcatc agttgagtca
     8341 ttatcaacga tgattatttc aagttttcca tctggataat tctgtccggt caattgttcg
     8401 atcagtcggg gcaggttgac tgcttcattt ttgaccgcga tgacaatgct gattgtgggt
     8461 aaatcagcgg ttctctgttg tcgtgggtga tttagtcccc agcgaaaaat gatgaggctg
     8521 actaaataga ttacaatgct tataaatgta atcaggtgaa ttattatcat tgcaacataa
     8581 tttggatatt attcaggagg ttcgttttca tagtaatcgc ttaggttttc gatcaagact
     8641 atatcctgac gtttttctac caccggcgta accttgataa aaaaccgcat tcctaaatag
     8701 aaacacacta aactggcgat aattaggact ggatggacat gaccatgcca ttcgtaaaac
     8761 ttagcgaatg ccggttggtg gctccaccaa accgaccagc cgttattaac agcatgtaaa
     8821 acgaacgaag gccagatcga gtttgcccgc caggctaaat accccaaaaa aagtcccatt
     8881 aggtaaatct ggacaaccca ccagggatta aaatgaacca gagcgaatac catcgctgag
     8941 agcaagactg atttggttat atcaccctga cgatattcta aaatgcgttg gaagaaacca
     9001 cggaacatca tctcctcgac taaaggagcg gcaaatacaa ccacaccgat aatcagcaga
     9061 gccgattgcc agtcggttat gagcataatc tcaccaattg acgcaaaact ctccggtaat
     9121 ggcaaaacga tctggatgag tcggtccaat tcatctataa tgactgtcaa actaaccgaa
     9181 acaatcatcg aggcgattat agaaggtgag gatacgcgtt tgattcttag cagttcaccc
     9241 aaattagtgt gcctccgcca agcccaaaag aaaatcggga ctggtaaaaa caattccgca
     9301 atcaataata acaactgagt agtttttgtc agattactcg tcggcacaaa aatcattatc
     9361 attaaggaaa ccagtgtcgc gcagattagc cccataatta atagtccaat tgctgtttta
     9421 attttgggga cgattattgg tggatgtggt ttcaattccg gctcaaaata ctggtgatta
     9481 tttatcatta atttgcatcc taatttttct caataaacaa attgtcatcg gttagttttt
     9541 tgatattggt ttgaattagc tctttgtcac tgacttgaaa accgcgcttg agaaaggcga
     9601 tttgtccatt gacattaatg cctaccagag ttggagtgat actagcctgg tagcgctttg
     9661 ccacctgtcc ataacgatcg agcagaaccg gcattttata gttcattctt tgtatatatt
     9721 ctttaacaat ctggggttct tcaccgatat tgaccagata aaaggcaatt tgtggatatt
     9781 cgattcttaa gctgtcgagg aaaggtattt cctgccgaca gggaatacag aaagttgtaa
     9841 agaagacgag gataaccggt gctttctcat tcggatgagc tttagcgccg actttatggc
     9901 taaggaaaaa atctgaaccg tcgagaacac tagcatagaa gctcggcgct accgtaccga
     9961 ttgctaaggt atcgacggtg acctggggtg aggctggggt taccgataac aatataattg
    10021 ctaaaacata gccaaggaaa gccaagttcg atcctcttac ttttgccgaa tattatttaa
    10081 aatgattcca actgccacgg caacattcaa agattcgcca taaccgtatt tattaatgcg
    10141 aattaatccc gtggcgcgct gtaatatttc aggcggtaag ccgtgcgact cacttccgaa
    10201 ggcaataaca aaacggtcat tcgttatttc aggtttctcg atggtgccgg acatatctgc
    10261 cgcccacatt tgggcattgt cccgccctaa aatcatctcg atccgagaca ggtcaacctc
    10321 cgttcgcacc ttcaaccgga aaagtgcacc catagcgccg cgaacaactt taggactata
    10381 tacatcaact gaatcaggag aaagcaggat agtttttaca tcgaaccaaa gtgccgtccg
    10441 caggattgta cccagattgc ccgggtcatt aatctgccag agatagagcg ccggataatc
    10501 gagaggcgcg agcaaattta cttcgctggg aatttttgca atagcgagaa ccccttccgg
    10561 attacggagc gcagaaatct gctcgatttc tgattgagta actacaataa tcgcgattcc
    10621 ctgtttatct gccagggatt tcaggcttag ccgttcggct tcgtcaatgc tttcggaaat
    10681 taaaatgcgc tccaggggaa aggtggcaat cagggcttct tggaccggtt tgattccatc
    10741 tatcacaaat agtccagtac gctcccgctc ctttttttgc tttaaagaac ggtaaagtgc
    10801 tctctcgtta cgcgtcagga tcattttcaa agataatagc gctcttttaa ttcgtataat
    10861 tttttggttg ggccagctag tatcaagata tcgtttggca ggataatcgt gtcggcgcgg
    10921 ggcgtgatga ccattcttcg gcctttatta tccaaccttt taattaaaat aatctgagca
    10981 tgataacggt tacgcagatc gagcgctttt agggtggaat taaccataga ttccggagcg
    11041 ctcatttcca gaatggaaaa atcctcatca atttctacga agcgagtttg atcgagatag
    11101 cgaatatgag ttgccagatc gccggccgtt tcgcgtttga aaatttcttg attatacaat
    11161 tggatgacgc gactacggtt aaccgttccg atcaacattc cggtggcggg atcagttacc
    11221 ggcaattcat caacattata acgcccaaac aatttcatta cgtaatcgag agtatcgtca
    11281 gttttaaccg atactacatt aggattcatc acatccgaga cgagtaccaa agtattcaaa
    11341 ttttgataat cggtaatgag cggttttaaa tccgaccatg agataaagcc gataatctta
    11401 tgctgttcat tttctaccca atagatattg gctgcgcctt ccgcaatgac tttcaccaga
    11461 gtttttacat ccatagttga atagacttta tggaattcat acgacataat ctgatcaatc
    11521 tttacatttt ttaagacatt cagctccttg ccgtcaaata attgaatgcc tctttgtctt
    11581 aattcgatcg aatagacggt ctcttttagc acataatgaa caattatata actcataatg
    11641 acggtgagca tcaagggtag aacgagatga taattgccgg ttatttcaac gatgattaat
    11701 aatgctgtca ccgggatgct gttgacggcg gcgagcatac cacccattcc cgcaatggcg
    11761 aaaccggcta cgtccagcga aagacccaat acctgattgc cgcccttagc caggaatgcg
    11821 ccaagcgccg cacccataag gagtgaaggg gcaaatagac cgccaaaacc acccgcggct
    11881 gacaacaatc ccgaaaataa aaatttaagg acgaacacaa ttgcaagaac ctgccaagta
    11941 taattattat tagcaatttc tcgaatggca tcatagccaa tgccaatgat ttgggggaag
    12001 aaaagcccga caatgcccaa tattagcatt aaaccggtaa gcctgagtaa cggtgcaaaa
    12061 cgacctttct gagcatcgaa aaaattgtgc attttacccg agaaacgaat aaaagccaat
    12121 gaaaataaac cggtgataat acctagaaca gcaaaatata atatattgag ggataaagcc
    12181 atttcaactt ttggcagagt caaaagcagt tgatcaccca gaaaaaccct cgagatgtag
    12241 tcggctgtaa tggctgagat tattaaagcc gaaaaggtaa gcgtctggaa attattaaat
    12301 agaacaattt ctatggcaaa aaaaactcca ccgatgggag cattaaaaac agcggctatc
    12361 gcaccggctg tccccgcggc taagaaaact cgcctttgat tttccgacac tcgaaaagct
    12421 tgaccgatta atgaaccact accggctccc agaaatgcga ccggcgcttc cggtcccaga
    12481 cttccacctg ttccgagaaa aatcgccgtc gcaataaaat taaaaatgat agtagtaact
    12541 cggattacac cccgtccgat gaaactggct ttgattactt ctactactcc gcgctgacgc
    12601 ggttgacgcg gccatagttt aatcattaag ccaacaatca gccctccgac cgccggaaaa
    12661 agagccatcg tccatttcgg caaattatta taccttgcag gtaaataatc gtatagaaaa
    12721 tagcttatca agtggattag tttgtaaaaa gccagagtca ccaatccagc caaaacccca
    12781 atcagaaagg caaaaaacag cagacggata tattcgttaa gtggattgtt ggcaatctgc
    12841 atccgcaggg agaagaaaaa gctggatatc cgcttggtta aagatttaac cttagcactc
    12901 acactaaaac tccgctatgt ttatcaatat caagtaatta tattttttat aaaatgtcat
    12961 ttgttatatt tatctaccag ttatcaaaga cacttcgcga atctatcata aaataaaatc
    13021 aaaaatcaag gcaaaattag tctgccggtg gctatcaaac aagtccggtg ttgatttaac
    13081 tgttatcatt tctccgctta tttcagaatc aaaccggcca aaatttaacc ttaaaaagcc
    13141 tccaaagaat tcccgccgaa aactcgggat ggtcgaagtt ttcatttcgt tataaaagga
    13201 gaagaaaaag taagttatag tatagcactc agcgggagta aaataactgg ttttgcggat
    13261 gactcaaata ctcgcacccg tcagcagtaa ccacgatcat ttcctcgacc gttgccactc
    13321 cgaaaccttc tatcggcaaa cgcggttcga tagtaaacac ttgaccgatt tccagcggta
    13381 aataaaccgt atcgccatag cgttcccagc gtggtcccaa catcccggca ccgtcatgag
    13441 ttgatcgacc tacctgatga ccaaggccgt gtggaaactc gggatagccg ttggcagtta
    13501 tataatcgcg ggcaatctga tcgatctcat agcccatttt tcctggtcgg atggcatcca
    13561 cagctcgacg gacagactcc agaagtaccg caaagccttt ttgaacaggt tcaggcgcgg
    13621 tttcttcatt atttcttaaa atgtaccaag tgcgctgaat atctgaacag tagccatctt
    13681 ttttcacacc gaaatcaatg ttgagcacgt gaccaggctg aatgactcgc tcggttggtt
    13741 cggcatgcgc cccagccgag agcggtccgg taaataccgc cggacaggaa ttcgattccc
    13801 aggcagtttg taccccaagt tcagctacca ttttcaagat aatatccgca atctcctttt
    13861 cggttcggcc tgatttaatt tgcggactga tttccgcata tattttttca gtaatttcta
    13921 tcgctctctt tattcgctga atttcagtgg gcgattttcg cccgcgtagc gccgcaacaa
    13981 ttttttcgga agaaataaaa cgctctcgat aaggcgtgtt tttagtatag cccatcagta
    14041 attcatacat accatgcgtt aagccatctg ccatataatc gttgatagag gtattgatag
    14101 cgattcgatt aggattaatt tcattcagtg cgttcagaaa agccgcgccg aaatcgtcaa
    14161 cgtaaatccg aatatcatca tataatccag tctgtttaat gcgttcctta tccagactac
    14221 caacaattgc gatggtttgt cctttggctg taatgatgaa agccgattgc caggtacaat
    14281 tagttccaac gatcaaatcc atacaggggt caggtaaagt ttcggtttcg cggacaaacg
    14341 tcatccacat atcaactccc tgttctttga gaatttcgat tgcctgtctg attttttctt
    14401 taaacattat ttctccaatc gaatttttgt ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnaaaagaaa agctggaatg aaacatctgg gcagagttca ttccagctac
    14581 ttttgggcat tttcctgagt gggcgacatc attgtgaggg tacgcctaac catctggggt
    14641 agtatggttt caaagaacac taattaagag gacaataatt gtgccaatag gattatatta
    14701 atattatcaa gtagttataa gtatttatga tctgctagaa tcgtggcgaa taggacactg
    14761 aattgtgttt atcccaccac gaaacgagcc cttttcaggg agcttatagg attttggcta
    14821 agaatttccc ggtttgcgag gcagggtggg ctgatacttc ttcaggagtg ccttcgcaga
    14881 caatatatcc gccgttatca ccgccctccg ggcctaaatc aatgatataa tcggcagttt
    14941 ttattacgtc tagattatgt tcgatgacga tgacagtatt gccttgatct accaaccgat
    15001 tcaaaacatt gaggagcatc ttaatatcat cgaaatgcag gccggtagta ggttcatcaa
    15061 gaatgtagag ggttttgccg gtgcctactt tgctcagttc ggttgataat tttacccgct
    15121 gggcttcgcc gcctgataat gtcgtagcct gttggccaag tttgatataa cccaagccga
    15181 cgtctattag agtctgaagt tttttattga tagaagggat atttctaaaa aattcaaagg
    15241 cttcacttat ggacatattc aaaacatccg caatactttt ccctttgtat ttaacttcga
    15301 gggtatcgcg gttataacgc agacccttgc attcttcgca aagaacataa acgtccggca
    15361 aaaagtgcat ttcaattttc ttaattccgt ccccgccgca aacttcacaa cgaccgcccc
    15421 gaacgttgaa tgaaaagcga ccggctgtat agccgcggat tttggattcc tttaggtttg
    15481 caaataggtc gcggatatga gtaaaagtgc cagtataagt tgccgggttg gagcgcggtg
    15541 ttcgtccgat cggcgattga tctatagcga ttactttgtc aatatattcc agcccttgaa
    15601 catcggtata tctcagtggt actttctggg aaccatagaa gttgcggtgc aaaattggaa
    15661 ataatgtttc gttgattagg gtacttttgc ctgaaccaga tacaccggta atgcaaatga
    15721 atttatttaa agggaaacac acatcgatgt ttttcaaatt attgccggtg gcgccatata
    15781 aactgatttg cttggcgttg ccggggcggc gatttcgtgg tattgggatt gttttagctc
    15841 cggagagata ttgaccggtc agtgagcgcg gctcattttt aatctcagcc ggagttccag
    15901 cgaataccac attgccgcca gtagcgccgg ctcccggtcc gagatcaatt atataatccg
    15961 ccgattcgat catttctcgg tcgtgttcca ccacaattac cgtgttccca ttatcccgca
    16021 gatgaagaag agtttcaatt aatcggcggt tatccctttg gtgcaaaccg atgctgggtt
    16081 catcgagaat atacagcacg cccattaatt gcgaaccaat ttgagttgcc aatcgaatac
    16141 gttgagcttc gccgccagaa agggtgtggg tcatacgatc cagagttagg tactctaccc
    16201 caacattaat taaaaaattg agcctagtgt gaatctcttt taaaatttga tgagcaattt
    16261 gttgttcggt cgggttgagt tcgagattaa cgaaaaattc tctcaacttg gtaattgaca
    16321 tagcgctcaa ctgattgatc gcaaatcccc cgatttttat tgccagactc tccggtttta
    16381 aacgggctcc ctgacattgg gggcataagc gataactcat aaattgctga atccacatgc
    16441 gaattccggt ggaagtcgtc tgcttatagc gacggcgtaa attaggaatt actccttcga
    16501 aaggcgtatg atactcgccg tcaattcgat ccgatttcaa attaatggaa agtttggtac
    16561 catcggaacc atataacaaa gcatttttag cttcctgagg taatttttta aatggtgtaa
    16621 caaagtcgaa gttatatttg cgcgccagtc cttttagtat tgccgagtac cagccgccgc
    16681 ggggctgttc acccaatggc acgatggcac cctggctgag tgttttggtc tcatccggta
    16741 caactaatgc cgggtctatt tccatcaaag ttccgagacc atcacaagtg ggacaagctc
    16801 catacggact attgaaggaa aacatacgag gttccaattc ctcgtagctg attccgcagt
    16861 cggtacaggc taatttttca ctgaaaatct gttcattttc gctattgatc acaactacta
    16921 gtaaaccgga gccgatttta agcgccagtt cgaccgactc agtcagacga tctttgacag
    16981 taggattaag actgaggcga tcaatgacca cttcaatatt gtgtttttta ttcttatcaa
    17041 gaacgggcgt gtgctcgact tcatagattt tcccgtcaat tcgaactctt aggaagcctt
    17101 cgcgacgaac ttcgcgaaac acttctcggt attcaccttt acgcccacgc accaagggcg
    17161 ctaaaatcat caggcgggtg tcttcgggcc agctaagtat attatcaaca atttgctgag
    17221 tagtttgacg ttcaatacgt ttgccgcatt ggtagcaata cggaatcccg atgcgggcat
    17281 acagcagacg caggtaatca taaatttcag tcaccgttcc tacggttgaa cgcggattgc
    17341 gggacgccga tttctgttca atagaaatgg ctggcgaaag cccttcgata aagtccacat
    17401 ccggtttttc cattaatccc aagaactggc gggcataggc tgatagcgat tcgacataac
    17461 gtcgctgacc ttcggcatat attgtatcaa aagccagtga agacttgccc gaacctgata
    17521 aaccggtaat gaccacgaat ttgtcacgtg ggatttttac gtcaatattt ttcagattgt
    17581 gttcgcgggc gcccttgatg acgatatgct ctgtactcat cagactttcc atttaaatta
    17641 tccgattaaa ttatcacctt attaccataa tgaaaaatat ttttcacgaa ttttctacaa
    17701 atttattttc cgttttagta ataaagtttc acactttttc tttcttcaat cactaatgac
    17761 tgattatatt tctatgttag tttaaatcaa tgtaggagaa gttaaatgac ccagactggc
    17821 ctcgatcagg aaactcggac gatgatcttg gacactatta attcactgaa aaatgaattg
    17881 ttgactcgcg aaaagatcct cgaactcgat gataaaaacg aattcccgct ggaaatcgtc
    17941 aaggaactgc tgagtgaccg catcggtttg cagatggtct tcattcctga agaattcggc
    18001 gggttgggcg gcggcgcact cgatacttat tacgtttcgg tggaactggc aaaaatttgc
    18061 cttggcgttg ccacggcttt tctggcgatt catcttggtg ccgaaccgat tcttttgttt
    18121 ggaaccgaag agcagaagca gaagtggctc gctaaaattg tcaacgaagc cgcaatcgta
    18181 gcttatgctg tgactgaggc tgatgctggt agcaatctcg acgcaatcaa gaccaaagcc
    18241 gaaccgatga cagatgaatc tgggaatatc gtatcctatc gtctgaatgg tagtaaacaa
    18301 ttcatctcca acggtgggta tgccgatttt ttaactttac tggcaaaggc tccggaaggt
    18361 gcgacttttt tcatagttga tggcaaatcc acgggattga aacgcggcaa accggaacat
    18421 aaacatggca tccgggcggc aaatacttcg cccctcaccc tggaaaatgt aatagtgccg
    18481 gctgaaaata tcgtcggtgg ggtacccggc gttggattaa aacaggctaa tgatgtcttt
    18541 tcctatacgc ggctgatggt ggcggcattt ggactcggag ctgggctttc ggctatggaa
    18601 aaagccatcc agttcgccaa aacgcgaatt cagtttggga cgccattgat tgaaaagcaa
    18661 ggctatacgc ataaattgct ggtacctaat gtcgtccgtt tgaccgcggc gcaggcatat
    18721 atcgaggaaa ttgccaagcg ggtggattcg ggcgatactg atattctggt cgaaggttcg
    18781 attgctaaat attttgcgac ggaagccgga aatatagccg cggaagccgc tattcaagct
    18841 ctgggtggct atggttatat gcacgattat gaagttgaga aaatcaaacg cgacgtgcgc
    18901 atcactatga tttatgaagg taccagtgaa atccagcaaa atattatcta cttgtatcgc
    18961 tggcgaacaa ccgttaaatc caaaggcgaa ttttatcaaa aaatggctca agagatgcga
    19021 actattgcgg aacaatcgaa cctggaatct gccaaaattg tggctcaagc gttggagctt
    19081 ctgaatgacc tgatcatgtt cgcccataat cagaaattaa ctcgccagca acacattcaa
    19141 ttcttgcttt cggatgttat cacctggtcg gaagttgccg cggctttggt acgcaaaacc
    19201 gccgaacatt tacgtgatgc attgcctgat gctattcaac tccaatccgt tctgcgaatt
    19261 tttgtagcgg aaatggcaag acttatctct aacaaaggta tggaaattat cctgggtagc
    19321 gaaactgt
//
LOCUS       DAOMJU010000037        18358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2879_length_18358_cov_18.454461, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000037 DAOMJU010000000
VERSION     DAOMJU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 18358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18358
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2879_length_18358_cov_18.454461"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /gene="lexA"
                     /locus_tag="PLO58_05125"
     CDS             complement(<1..594)
                     /gene="lexA"
                     /locus_tag="PLO58_05125"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQO74206.1"
                     /translation="MAEKLSPKKQRFVNFVHDFTETNKRAPTFLEIMHALQIKSPGTV
                     SWYVRELEKAGALRRSGPPNAKRALSVSETESASTLPLLGLIAAGYPLEAIENQETIA
                     VPPAYIHPSNYVLKVKGDSMIDDNIQDGDYIIVRQQAQAESGQTVVAFINNEATLKRY
                     YPKAGGVELHPRNPAYPVIKVKPGDEFRLGGVVLAVWR"
     gene            complement(815..1447)
                     /locus_tag="PLO58_05130"
     CDS             complement(815..1447)
                     /locus_tag="PLO58_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQO74207.1"
                     /translation="MINLLIVDDHPLIRQGLRQTFLDTNDIRVSGEASDSASALAEIE
                     NHNYDAVILDLSLPGRSGLDLLKQIKNLSPSLPVLILSVFPESQYAIRVLKAGADGYI
                     SKESHPDELIHAVRKIVTGGKYISPTLAEKLANSLTEATQNPTHEILSDREFQTMLAI
                     ASGKTVKEIAAELNLSVKTISTYRTRILRKMHLKNNAEIICYAVRLGLVQ"
     gene            complement(1447..2631)
                     /locus_tag="PLO58_05135"
     CDS             complement(1447..2631)
                     /locus_tag="PLO58_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HQO74208.1"
                     /translation="MNLPDSFTSDESKTREQLLWELQQLRNRINQLEAADLERRRIEK
                     SLNKYEKNFRELVNLLPQTIYEMDKNGKFRFTNQVGFETFGYNQQDIDTGVNFSQLFI
                     PEEREKLNRNIQKIYNGSDLEGHEYTALRKDGTTFQALIYSSPIVYNNKPIGLRGVVI
                     DITDRKKVEQDLKDSRDQFRNLSAHLQSVREEERSYIAREIHDELGQALTALKMDLIW
                     INKRLTTAQSEIAEKISTMFDLIDKTIQTIRRISTDLRPGLLDDLGLTAALEWYCEDF
                     QNRTGIHCNLHLRPAEITIDQERSIAIFRILQETLTNVARHAHASEVKVNLGIKDHAL
                     EMKIVDNGIGITDEQVNDPQNLGLVGLRERVYPWNGHIAIKGRPQKGTTVLVFIPLDG
                     NI"
     gene            complement(2634..3119)
                     /locus_tag="PLO58_05140"
     CDS             complement(2634..3119)
                     /locus_tag="PLO58_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74209.1"
                     /translation="MNLKKKISIGGMIELRDLMMVSIFSFPDQPGSAGKVLTYFGQRG
                     ISVDFITESRNLEGSADLIFCINQKYKSDITQTFQELSAIIPIKDTKITEPVAIVSFY
                     GPHFREKPAIAGTICQTLGEAGINILGISTSISSVSCVINEHQVDAARQAITANFNLP
                     E"
     gene            3323..5896
                     /locus_tag="PLO58_05145"
     CDS             3323..5896
                     /locus_tag="PLO58_05145"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HQO74210.1"
                     /translation="MSIVNNSLYCIPIYNPYYSLPFPSQAEINELIDNLLNSLKEGTQ
                     KGVYHFDQMIYPLCEYLKSHPEKTDLVRQLVEEKKIYFGPFFIQLHSGHISGELLIRN
                     LLIGHQEGDKIGDILKYFHLPAMRGRNSQTPQILAGFNIDAVLIDYDIKLMDKNTTEF
                     IWEGLDGTQLLVSRVVFLNPNRLNLAKSKIQETFREYFSHNPNRVLVYLFNDKKSWET
                     MPTYIEKLASVFSSDLKIESLADCIWEIKEQIDSREISEYKGEMCLEKTEDCTETQFD
                     YYLRHSVLIGLSNHRLENLIQYFVEPWSLIMKYLQLKIRPKATNHIWEEFLKNQTFTY
                     LVDTNPNKFIQSLSTTQTGLMNRIEEQVKDLITAVVNNIHIDDYNPNNIYFTVFNSLP
                     YNRSEIVEFVMEMPSLINRDTIVAKEVEGREISFRVLHKETETLFKYDKADKAKYRCL
                     VELRDLPGMGWKTFEIDLNGRPKSYPAVPISAERNSLENDFLRVEINQNGTLEIFAKE
                     TGEFFSEVAYFQDEIDLGRFSVEDKTTTHIPLTTKKLHPQIKLLYNSPKAAAYKIEYF
                     WEIPEMFNWKNSRRSPRHETIKISEIVTLSSLSRMIDLKIEINDHACDHMIKICFPIE
                     FVPENTYTDGFFSVETRPLNGLQNLHCQAFSMGNFVGVSNESGGFAIFSDGINEYQLI
                     KGKHNVLTLTLVRDFPIVRSKKTGGPILSRNEKTEVHLSIYPRLSDWTSSDILTEAII
                     FNNPIIIRQMDQTNGYLPAKMQFLKVTPDTLFYSALKPTEDEKDVVLRLYNPTRNIIV
                     GEIATYIPIKAARYLTVEEHIIAPLEISDPYRLKVKVFPQKIMTLKIIFDRLPINSSS
                     S"
     gene            5980..>6206
                     /locus_tag="PLO58_05150"
     CDS             5980..>6206
                     /locus_tag="PLO58_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74211.1"
                     /translation="MFANQDKSSIQKSEEFVSELSRNHITAQKEVPLAKFTTFGIGGN
                     ARLLVIVENTEKLEAVIKLATAFQVPFRIIG"
     assembly_gap    6207..6306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6307..6662
                     /locus_tag="PLO58_05155"
     CDS             <6307..6662
                     /locus_tag="PLO58_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74212.1"
                     /translation="TMGNVEQARIVAQEWKSRKSFQPRRSAGCIFKNLSPEAQKQFNL
                     PTSSVGYLIDKVLNLKGLRIGGAVISPKHAAFIENDGTARADDVYKLIKIIKAKAQET
                     LGLELETEVELLGKF"
     gene            6680..7939
                     /gene="murA"
                     /locus_tag="PLO58_05160"
     CDS             6680..7939
                     /gene="murA"
                     /locus_tag="PLO58_05160"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQO74213.1"
                     /translation="MSRFIIEGNRNLSGEIKVAGNKNAALPIIAATVLTDEPCILENL
                     PDISDVRSMLAILSDLGKSVEMLSAHKCQITGSVNKSRLNDKLASTLRASILYLSVLL
                     ARTGEVEMVPPGGCVIGRRKVDQHFDALQALGVKVNIIEHGYRLILKRPKPNYVFLRE
                     ASVTATENAMMLAAMTEGKTTLDNAAGEPHVQDLAKVLVKMGAKIQGAGTNRLTITGT
                     KKLKGFRHRIMPDHIEAGTFAIAAACTGGEVVIHDANRENLTIINLILDQMRVKLSFL
                     DEKTLVIKPSKNIRAIPKVQVGVWPGFPTDLMSPMVVLATQARGTTLCHDWMYESRMF
                     FVDKLVTMGAQIVQCDPHRVLVSGPTHLKGQEIVSPDIRAGIALVIAALIARGKSKIE
                     KAELIDRGYEDIVPRFRQLGAEINREQ"
     gene            8045..10606
                     /gene="glgP"
                     /locus_tag="PLO58_05165"
     CDS             8045..10606
                     /gene="glgP"
                     /locus_tag="PLO58_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQO74214.1"
                     /translation="MKPIQSFTVKPILPKKLEELRELAYNLWWTWNADARDLFRRLDM
                     DLWEETRHNPVALLNRVEQDVLETRAQDDSYLYQLRRVYDKFKQSLNSPTWFEKSGKY
                     NSHLNIAYFSMEYGITECLADYSGGLGVLAGDHLKSASELGIPLQAVGLSYQYGYFQQ
                     VLNVDDYQEEEYPVNDFYNSPLTLVKGSDGKQLLIKVPFPGRLVYARIWKAQVGRVPL
                     YLLDTNIAENSEADKKITAELYGGNTETRIQQEIILGMGGFQALQILDATPFICHMNE
                     GHSAFSGLERIRNVVKNNGLTFYEALEIIKAASVFTTHTPVQAGIDLFHPDLIKRYFD
                     EYCREVGIEINELLALGRNDPDDNSEHFSMAILAINLSSKINAVSKLHQQVAQEMWES
                     LWPDILTTEIPIDYVTNGIHQASWISQEMSDLFDRYLGPDWRREPASKTIWNKVDKIP
                     DGELWRTHERRRERLVAYARSELYRQYVTLGKPQNIIARVKSILSTDALTIGFARRFA
                     AYKRAYLLFTDPERLAKILTNKNFPVQIIIAGKAHPQDEEGKQVIRRILKLSQEEPFY
                     QCVVFLENYDMNMARYLVQGCDLWLNTPRRGLEACGTSGMKAVANGGLHFSTIDGWWD
                     EVYTPGIGWAIGTRDIYEDIDYWDRQEANILYNVLENEIIPSFFERDNDDLPRDWIAM
                     IKASMKAICPQYNSNRMVCEYLDQIYYPAAVRANELSKDNYAVAKELAHWKEKMRKNW
                     DLVRFLKVTSGSIEELSVNSQLEIRAEIYLDGLQVDDVDVQVYYGRVDPNGDIIDGKF
                     SSMKFETKVDSKKYVYVDKINNWESGLNGYTIRIIPKHPALAYPFEDGLIHWFDE"
     gene            10665..11432
                     /locus_tag="PLO58_05170"
     CDS             10665..11432
                     /locus_tag="PLO58_05170"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HQO74215.1"
                     /translation="MNLITNEVARKRISQGEQVVVLTGAGISAPSGIPTFRGENGLWT
                     KLSPQELASFESFYLNTAMVSEWYKHRREIIEVCQPNAGHYALVELAKMVSKFTLVTQ
                     NIDGLHQRAGSRDVLELHGTIMENYCIRCGQQYSATEFDEIYNSSFDHIPRCYCGGLI
                     RPNVVWFGESLPKQAYEEAYRASQQADLFLSVGTSAQVRPAADLPLIAKENGAFLVEI
                     NIVETVLTPFTDLFLKGAADEILPQFNREYQYLLMQV"
     gene            11459..13318
                     /locus_tag="PLO58_05175"
     CDS             11459..13318
                     /locus_tag="PLO58_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO74216.1"
                     /translation="MKNNITVKSIETLFLIGLILVLSNCAYYNTYYNAEKYFREAEKE
                     FKTLPDEKISPALKKKFDTAIEKANKVITQYPESRWVDDSYYIIAMSHYYKGDYRIAR
                     QKFEDFAVKFPQSKLYPEAMVWYGRNLWKLDERELAFFQWKKIMNLTDDYYLLAELYL
                     SIGELYFKDSAYDSALFYYQKATEVGGSFDIAAEAQFRIAEIDLALNRPQEAIKNLKK
                     IDQFSPSLILRDKMQVLLVRIYRESGQYDKAIDLINEKLNDPANEKIWGDLELQLGLI
                     YLAQQDYDAAISRLTKVIEDYKGKPVAAEASYHLAELYMTQIHDYTKAAVQYDNVVKI
                     EVNTLRAFECRNKSSEIKRFNTIYNRLAALSEQVAAIDLNPPTENDTIGESLPVDQEQ
                     SELKKALEKKNTTQKKTIDTLAVFKEYYTTLYEIAEIFYFSFNQPDSAIYYFEKVAYE
                     IPFNSLRDKALYSLYRIASDRQNIEKANEYANQIKQWFPESIYLAEIEKRPLNTSARE
                     VEADSLLVKAENLDQTDLHSAITIYEKLAKEYTDCQAAEKAVMYLAYFYHHRLFDLNN
                     ALTWYKYYLDTYPNGQYYQIMANAYDQLKEIEDALAQGSEAEENEPQKPEKVE"
     gene            13315..14082
                     /locus_tag="PLO58_05180"
     CDS             13315..14082
                     /locus_tag="PLO58_05180"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HQO74217.1"
                     /translation="MRVLKDTTVLSNRMVATDTYLMEFAFPEISQSAQPGQFVNFYFP
                     ERPQLFPRPFSIAGVNRENLTILYKKVGCLTSIMSQWQPSQKIRVLGPLGNTFNIKYN
                     NHVLIAGGVGLAPLLFLNQKLANLNYKIWNFVGVRSKDELPPLSLLSGEKYIATEDGS
                     VEYCGTVVDCLRNNLGAIPHPFSVYACGPEAMLSALWHLSRENHFDLQLSLEKLMACG
                     LGICQGCAIPHSHPSEQPLRLVCQDGPVFNANELDLG"
     gene            14079..14993
                     /locus_tag="PLO58_05185"
     CDS             14079..14993
                     /locus_tag="PLO58_05185"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HQO74218.1"
                     /translation="MTDLHIKLREDFILRNPLLTASGTYGYGDEIADLINPELYGGIV
                     TKSISLEPRVGNPPPRIAEVTSGMLNSIGLANIGVDKFLEEKEEFLAAYNGKAIVNIA
                     GRKDEEYVQVVKKLSDRQWIAAFEINVSCPNVSEGGIEFGRDPLILGQLVKKLRHATD
                     KFLIIKLSPNVTDIRVLALAAQENGADAISMINTVYGAAIDIHTRRPKLNTVVGGLSG
                     PAVKPIALAHIIKTYKEVDIPIIGIGGISCGADVIEFLLAGAHAVQIGTANFFNPKVC
                     GEILDFIEQYCTENKISCLRDIIGRVEV"
     gene            14957..15451
                     /locus_tag="PLO58_05190"
     CDS             14957..15451
                     /locus_tag="PLO58_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQO74219.1"
                     /translation="MSARYNRTSRSLSLIVILLLFMMLGFMGCASAPRYLYTSPKTGA
                     TNDDKNTLLLLKNSNRTAVLTASYYGADFHGKLTSSGEVFDMNGMTAAHKSLPFGTVL
                     QVTYPPTGQSVTVVVNDRGPFVPGRDLDLSYAAARKIGLVNDGTGKVKVKVLKWGNND
                     SPKQ"
     gene            15467..16270
                     /locus_tag="PLO58_05195"
     CDS             15467..16270
                     /locus_tag="PLO58_05195"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQO74220.1"
                     /translation="MNNFASRMLINIIGIPAILLLIHLGGIPFVLFITVMVFIAQVEL
                     YLLMRKKGIAPLLLPGVLAGVIWQLASFYLPGALLLYLLIGLVVLLLLASLVRQIKNV
                     TVDLASTLFGFVYLPVLTSTIILMRNIPGRGRSIVLLLFVTIWVCDSLAFIIGKWLGR
                     RKIAPKISPNKTYAGCIGGLFGAVLTVLIFYFLGWAPDFMSIMQLIIFGILAGVFGQA
                     GDFVESAFKRDAEVKDSSNLLLGHGGVLDRFDSFFIAAPVIFLYIITIL"
     gene            16318..17826
                     /locus_tag="PLO58_05200"
     CDS             16318..17826
                     /locus_tag="PLO58_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQO74221.1"
                     /translation="MSINNGNNDLVEYLHSLSGEIDPKQGSVAIILAAGHGKRIKSER
                     SKMLHEIWGVPTVVRVANAASKGLASNNQIIVVGIKACEVAESAGKRANRIFVYQTEQ
                     KGTGHAVKEALSAISQNGHIKDIYIFPGDMGLITPAVVSELKRRFEQSGCGLALLTGN
                     YKGEPEANSYGRIIRVPARTATGEPSGEDEGRVIEIKEHKDILAIPESGFYTTVYNGK
                     KYQFKRSELLGITEYNTGVYAMKFDLAQRFIHQLTPDNVQGELYLTDIIGLFNANKIE
                     VEAYPVDDESAVIGFNVKSVLQEMENIARRKVWKLLRDIIYIEDENDFFIADEVVIRI
                     LEMDKINPPLDIEIGKGVHISSGVHLERGVKIRAGSLLNGNIHLGVGTVVQENVSLST
                     YPNQQMTIGKNCVIMRGDLIKGNITIGDNTSIESSVIITGSSEYPTIIGKNVVIKGTT
                     YIFGSIIEDDLFIEHSVLKCKRVERTVRKDGTIQPVRWVMPQPQGLDIVRDL"
     gene            complement(18104..18241)
                     /locus_tag="PLO58_05205"
     CDS             complement(18104..18241)
                     /locus_tag="PLO58_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HQO74222.1"
                     /translation="MIAEQIAEYIRFYNEERLHSALFYLTPKEVFEGKMEQRLEERQK
                     S"
BASE COUNT         5269 a         3550 c         4050 g         5389 t
ORIGIN      
        1 ccgccacacg gccagcacca ctccgccgag tcggaattca tctcccggtt tgactttgat
       61 aaccggataa gccggattgc gtggatgcag ttcgacacca ccggctttgg gataatagcg
      121 ttttaaagtg gcttcattat tgataaatgc tactacggtt tgtccggatt cagcctgagc
      181 ttgctgacga acgatgatat aatcgccgtc ttgaatattg tcgtcaatca tcgaatcgcc
      241 cttgactttc aaaacgtaat tagacgggtg aatgtaggct ggtggcacgg cgattgtctc
      301 ctggttttcg atagcttcca acggataacc agccgcaatt aatccgagca ggggcaaagt
      361 gctagcgctc tccgtttcgg aaacggacaa cgcacgtttg gcattagggg gaccggaacg
      421 ccgaagagca ccggcttttt ctaattcgcg cacgtaccag ctgactgtac ccggcgattt
      481 gatttgcaag gcgtgcataa tctcgaggaa agtcggggca cgtttgttgg tttccgtgaa
      541 gtcgtgaacg aaattcacga aacgctgttt cttgggcgaa agtttttcag ccattttgca
      601 tttcctctca ctttatcaat tactcgtaag ttactcgtaa gtaatgtagc aagtttttag
      661 ctgaaaagca attggaaaat tggaaatttg ggatttcaaa tcaaataata actaccaacg
      721 tcaatcaata atcccgcagt agcagatctc gtgtcattca accaataaat gactacgaat
      781 cgcaatgaat aatcattgtc ttataaaatt aactctactg cactagtccc agccggacgg
      841 catagcagat aatttcggcg ttatttttca ggtgcatttt gcgtagaatg cgagtgcgat
      901 aagtgctgat agttttgacg ctgaggttca gttcagcggc gatttccttg acggttttgc
      961 cgctggcgat cgctaacatt gtttgaaatt cgcggtctga aagaatttcg tgggtgggat
     1021 tttgagtggc ttcggtaagg ctgttagcca atttttccgc aagtgtggga gaaatgtact
     1081 taccaccggt tacgatttta cgaacggcgt ggattagttc atctggatga ctttctttgc
     1141 taatataacc atcggcgccg gctttcaata cccgaatagc atattgactt tcagggaaaa
     1201 cgctgagaat taataccggt aaagaaggtg aaagattttt gatttgtttg agaagatcta
     1261 agccactgcg gccgggcaat gaaagatcga gaattaccgc atcgtagtta tggttttcaa
     1321 tttcagcgag ggcggaagca ctatcgctcg cctcgccgct aactcgaata tcgttggtat
     1381 caaggaaagt ttgacgtaat ccctggcgga tcaaaggatg atcgtcaact attagcaaat
     1441 ttatcattag atatttccat ccaatgggat aaagaccaga acggttgtcc ctttttgcgg
     1501 gcgaccttta attgcgatgt ggccattcca gggataaacg cgttcgcgca atccaaccaa
     1561 gcccagattt tggggatcat tgacctgctc atcagtaatt ccgatgccgt tatcaacaat
     1621 tttcatttct aaggcgtgat ctttaatacc gaggttgact ttgacctcgc tggcatgggc
     1681 atgacgagcg acattcgtca gagtttcttg taaaatacgg aaaatcgcaa tactacgttc
     1741 ctgatcaatc gtgatttccg ccggtcgcag atgtaggtta cagtgaatac ctgtccgatt
     1801 ttgaaaatcc tcacaatacc attcgagagc ggcggtcaag cctaaatcat cgagcaaacc
     1861 aggtcgcaag tcggttgaaa tccgccgaat agtttgaatt gttttatcaa ttagatcgaa
     1921 cattgtgctg attttttcag caatttcgga ttgagcggta gttagtcttt tattgatcca
     1981 gatcaagtcc attttcaaag ccgttagtgc ctgaccgagt tcgtcgtgaa tttcgcgggc
     2041 gatataagag cgttcctctt cgcgtaccga ttgcaaatgc gccgaaaggt tacgaaactg
     2101 gtcgcgcgaa tccttcaaat cttgttcgac ttttttccgg tcggttatat caatgaccac
     2161 accgcgcaag ccgatcggtt tgttattgta gacaatagga ctggaataga ttagggcttg
     2221 gaaagttgtc ccgtctttcc gcaaggcagt atattcatgt ccctcgagat cgctaccgtt
     2281 ataaattttt tgaatattac ggttaagttt ttcccgttct tccggaataa aaagctggga
     2341 gaaattaact ccggtgtcaa tatcctgttg attatatccg aaagtttcaa atccgacttg
     2401 attggtaaag cgaaatttgc catttttgtc catttcataa atagtttggg gtaggagatt
     2461 gactaactcg cgaaaattct tttcgtattt attcagggat ttttctattc ggcggcgttc
     2521 gagatcagcg gcttcaagct ggtttatccg attacgtagt tgctgtaatt cccagagcaa
     2581 ctgttcgcgg gttttacttt cgtcactagt aaatgaatca ggaagattca tttctactcc
     2641 ggtaagttaa aattcgcagt tatagcttgc cgagcggcgt caacctgatg ctcgttaatt
     2701 acacagctta ctgaagaaat actggttgaa attcctagta tattgattcc agcttcgcca
     2761 agtgtttgac aaattgtccc ggcgatagct ggtttttcac ggaaatgagg gccataaaaa
     2821 gagacaattg ctactggttc agttatttta gtatccttaa ttgggataat ggcagaaagt
     2881 tcctgaaacg tctgggtaat gtctgatttg tatttctgat tgatacagaa aataaggtcc
     2941 gccgagcctt ccaaattgcg gctttcggtt ataaaatcaa cactaatccc tctttgcccg
     3001 aagtaagtca aaacttttcc ggctgaaccc ggttgatcgg ggaatgagaa aatcgagacc
     3061 atcatcaagt ctcttaattc gatcattccg ccgatactga ttttcttttt taaattcata
     3121 ttggctctgt ttgcaaattg ttgaaaaata ggttaattat ttgaaaaaat ccagtccgat
     3181 ttttaacagc cgataaatta ataccaatct tcatctatta attttaaaaa ataattgaaa
     3241 atttcactaa atatgtgata tattttagaa aacaagttgg attgggcaaa ttcggcttgt
     3301 atgaaattaa aaagtagatg taatgtcgat cgttaataac agtttgtatt gcataccaat
     3361 ttataatccc tattattcat taccttttcc ttctcaagcg gaaataaatg agttgattga
     3421 taatctttta aatagcctga aagaaggaac gcagaaaggg gtgtatcatt ttgaccaaat
     3481 gatttaccct ttgtgcgaat atttgaaaag tcacccggaa aaaacggatt tagttcgcca
     3541 attagtcgaa gagaaaaaaa tttatttcgg accgtttttc attcaacttc attccggaca
     3601 tatttccggt gaattgttga ttcgtaatct tctcattggc catcaggaag gggacaaaat
     3661 cggtgacatt ttaaagtact ttcatttacc agcgatgcgc ggacgcaatt cacagacgcc
     3721 ccagattttg gctggtttca atattgatgc tgttttaatt gactacgata taaagttaat
     3781 ggataaaaat accaccgagt ttatctggga aggcttggat ggtacccaac tattggtcag
     3841 tcgcgtggtt ttcttaaatc ctaatcgttt aaatttagca aaaagtaaaa ttcaggagac
     3901 gttccgcgaa tatttcagcc ataatcccaa tcgagtcttg gtttatctgt tcaatgataa
     3961 aaaaagttgg gaaaccatgc cgacttatat tgaaaaattg gcttcggttt tttcttctga
     4021 tttgaaaatt gagtctctgg ccgattgtat ttgggaaatt aaagagcaga ttgattcgcg
     4081 tgaaataagt gaatacaagg gggaaatgtg tttagaaaaa actgaagatt gtaccgagac
     4141 tcagttcgat tattatctaa gacatagcgt gttgattggt ttaagtaatc atcgtcttga
     4201 gaacctaatt caatattttg ttgaaccgtg gtcattaatt atgaaatatt tacagctgaa
     4261 aattaggccg aaggcgacca accatatttg ggaggaattc ctaaaaaacc agacatttac
     4321 ttatctcgta gatactaatc ccaataaatt tatccagtca cttagtacca ctcaaactgg
     4381 actgatgaat cggatcgaag agcaggtaaa ggatttaatt acggcagttg ttaataatat
     4441 tcatattgat gattataatc ctaataatat ttactttacg gtatttaatt ctttgccata
     4501 taaccgatct gaaattgttg aatttgtcat ggagatgcca tctttgatta atcgggatac
     4561 gatcgttgcc aaagaggtgg agggacgtga aatttcgttt cgagttctcc ataaggaaac
     4621 tgaaacacta tttaagtacg acaaggcaga taaagcgaaa tatcgttgtt tagtagaatt
     4681 gcgtgacctg cccggaatgg gctggaaaac ttttgaaatt gatctgaacg gaaggccgaa
     4741 atcctatcca gctgtgccga tcagcgccga gcgcaattcg ttggagaatg atttcttacg
     4801 agttgaaatt aatcagaatg gaacgctgga aatatttgcc aaagaaaccg gtgaattttt
     4861 ctcggaagtt gcctattttc aagatgaaat tgatctggga cgattcagtg ttgaggataa
     4921 aaccacgact catattcccc ttactacaaa aaagctccat cctcaaatca aattgcttta
     4981 taattctcct aaagccgccg cctataaaat cgaatatttt tgggaaattc cagaaatgtt
     5041 caattggaaa aattcgcgtc gcagtcccag gcatgagaca atcaaaatta gtgaaatagt
     5101 aaccttaagt agcctctccc gaatgattga tttaaaaata gaaattaatg atcatgcctg
     5161 tgaccatatg ataaaaattt gttttcccat tgaatttgta ccggagaata cttatactga
     5221 tggctttttt tcggttgaga cgcgcccgct taacggcctg caaaatcttc attgccaagc
     5281 atttagtatg ggcaattttg tcggagtttc caatgaatcg ggaggatttg ccatatttag
     5341 cgacggcatc aacgaatacc aactaataaa aggcaagcat aacgtcttga cattaacctt
     5401 ggtccgtgat tttccaattg ttcgatcaaa aaaaaccggc ggccctattt tatcacgcaa
     5461 cgaaaagaca gaggttcatt tatctattta tccgcgatta tccgattgga cgtccagcga
     5521 tattttaact gaggctataa ttttcaataa cccgataatt atacgacaga tggatcaaac
     5581 aaatggatac ttaccggcga aaatgcagtt tttaaaggtt accccggata cattgtttta
     5641 ttcggctctc aaacccactg aggatgagaa agatgtagtc ctcagattgt ataatccaac
     5701 ccgcaacata atcgttggcg aaatagcaac ttatattcca atcaaagccg cccgttacct
     5761 tacggtcgaa gaacatatta ttgccccgtt ggaaatttcc gatccttatc gtttgaaagt
     5821 aaaagttttt ccacagaaaa taatgacctt aaaaattatt tttgatcgat tacctattaa
     5881 ttcgtcaagt tcttaatttt atccttttac taaaggctat ttgtcagaat tatgataaaa
     5941 atttataata attaacgtca ggtagtatta atttagcacg tgttcgctaa tcaagataaa
     6001 tcaagtatcc agaaatcaga agaattcgtc tctgaactaa gccgaaatca tatcactgct
     6061 caaaaagaag tgccgttagc caaatttacc accttcggaa tcggtggcaa tgcacgttta
     6121 ctggtgattg tcgaaaatac cgaaaaactc gaagcggtca tcaaattagc cactgccttt
     6181 caggtaccat ttcgaattat tggttgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnntgac gatgggcaat gtcgaacagg ctcgaatcgt tgctcaagag tggaaaagta
     6361 gaaaatcctt ccagcctcgt cgttccgccg gttgcatttt taaaaatctc tcaccagaag
     6421 cccaaaaaca gtttaaccta cctacttctt cagtaggtta cttgatagat aaggtgctga
     6481 acttgaaagg attgcggatt ggtggcgctg tcatttctcc aaaacatgcc gcctttatcg
     6541 aaaatgatgg aactgcccgg gctgatgatg tctataagtt gattaaaatc atcaaagcca
     6601 aagcccaaga gacactcgga ttagaattgg aaactgaggt cgaattgctt gggaaatttt
     6661 aacgttatga aaggtaatta tgtccagatt tatcattgaa ggtaatcgca acttatccgg
     6721 tgagatcaag gtagccggga ataaaaatgc ggcattgccg attatcgccg cgaccgtttt
     6781 gaccgatgaa ccctgtattc tggaaaattt acccgatatt agcgatgttc gttcaatgct
     6841 agcaatcctg agcgatttag ggaagtcggt ggagatgcta tccgctcata aatgtcaaat
     6901 taccggatcg gtcaataaat cgcgcctaaa tgataaatta gccagtactc tacgcgcttc
     6961 cattctatat ttaagcgttc tactggcgcg tactggtgaa gttgaaatgg taccaccggg
     7021 cggttgtgta atcggacggc gtaaagttga ccaacatttc gacgcgttac aggcgttggg
     7081 agtaaaagtc aatataattg agcacggcta tcggctaata ttaaaacgcc ctaaacctaa
     7141 ttatgttttt ctgcgcgagg cttcagtaac cgctactgaa aatgcgatga tgctggcggc
     7201 aatgacggaa gggaaaacta cccttgataa tgcggctgga gagccgcatg tccaagactt
     7261 agctaaagtt ttagtgaaaa tgggcgctaa aattcaaggc gccggtacca atcgcttaac
     7321 aattaccgga accaaaaaac taaaaggatt tcgacatcgc attatgcctg atcatattga
     7381 agccggcacg ttcgcgattg ccgcggcttg caccggcggt gaagtcgtta ttcacgatgc
     7441 caatcgtgaa aatctgacga tcatcaatct tatccttgac caaatgcgag taaagttatc
     7501 cttccttgat gaaaaaacgt tggtgattaa gccgtccaaa aatatcagag ctattccgaa
     7561 agtccaggta ggagtctggc cgggtttccc aactgatctg atgagtccga tggttgtatt
     7621 ggcaacccaa gccagaggga cgactctctg ccatgattgg atgtacgaat cccgtatgtt
     7681 tttcgttgac aaactggtaa cgatgggcgc tcaaattgtg cagtgtgacc cgcaccgcgt
     7741 gcttgtttca ggtccaacgc atcttaaagg gcaggaaatt gtcagtcctg atattcgggc
     7801 tggtattgcg cttgtaattg ccgccctaat cgcccgaggt aaaagtaaaa tcgagaaggc
     7861 tgaactgatt gatcggggtt atgaagatat cgtcccacgc tttcggcaac tgggagccga
     7921 aattaatcgc gaacaatagt ggtttttcta tcgaatttta ttactttgtg gttgttttca
     7981 ttattttgta ggaaagaaga caattgatta atttaggcac tgatttttaa agctgaggaa
     8041 attaatgaag cctattcaat cctttacagt taaaccaatt ctgcctaaga aattggaaga
     8101 attgcgtgag ctggcttata atttgtggtg gacctggaat gccgatgctc gcgatctctt
     8161 taggcggctg gatatggatt tgtgggaaga gacgcgccat aatccagtgg ctctattgaa
     8221 tagagtagag caagatgtac tcgaaacgcg tgcccaagat gatagctatt tgtaccaact
     8281 gcgtcgggtt tatgacaaat tcaaacagtc cttaaactca ccaacctggt tcgaaaagag
     8341 cggcaaatac aattcccacc tcaatattgc ctatttttcg atggaatatg gcattaccga
     8401 atgtctggcg gactactcag gaggactggg tgtattagcc ggcgaccacc ttaaatcagc
     8461 ttcggaactt ggtattccac tccaggctgt tggactttca tatcagtacg gctattttca
     8521 gcaagtctta aatgttgatg attatcaaga agaagaatac cctgtcaatg acttttataa
     8581 ttcaccgctg actctggtaa aaggttctga tggtaaacaa ttactgatta aagtgccttt
     8641 tcccggtcgt ttggtttatg ctcggatttg gaaagcccag gttggtcgcg ttccgctata
     8701 tttacttgat accaatattg ccgaaaattc agaagcagat aaaaagatta cagctgaatt
     8761 gtacggtggc aataccgaaa cccgaattca gcaggaaatt attttaggaa tgggtggttt
     8821 tcaggctctt caaatactcg atgccacgcc atttatttgc catatgaatg aagggcactc
     8881 ggctttttcg ggattagagc ggattcggaa tgttgtaaaa aacaacggtc tgacatttta
     8941 cgaagcgctt gaaattatta aagcggcttc agtttttacc acccatactc ctgttcaagc
     9001 cggaattgac ctttttcacc ctgatctgat aaaacgctat tttgatgagt attgccggga
     9061 agtaggaata gaaattaatg aacttttagc gttgggtcgt aacgatccgg acgataattc
     9121 cgaacatttt tcgatggcaa ttctagccat aaatttgagt agtaagatca atgctgtcag
     9181 taaattacat caacaggttg cccaggaaat gtgggaatct ctctggccgg atattctcac
     9241 tacagaaatt cccattgact atgttactaa cggaatccac caagcctcct ggatttcaca
     9301 agaaatgtct gatttatttg accgttatct cggaccggat tggagacgtg aaccggcgag
     9361 taagactatc tggaataaag tagataaaat tccggacggc gaactctggc gcactcatga
     9421 acgtcgccgg gaacgtttag tagcttatgc ccgcagtgaa ttatatcggc aatatgttac
     9481 tctcggtaaa cctcaaaata taattgcgcg agtaaaaagt attttaagca cggacgcttt
     9541 gacaattggt tttgcacggc gttttgccgc atataaaaga gcctatctgc tcttcactga
     9601 tcccgaaaga ttagctaaga tattgacgaa taaaaatttt ccagttcaga ttattattgc
     9661 tggtaaagct catccccaag atgaagaagg taaacaggtt attcggcgta ttttgaaact
     9721 atcccaggaa gaaccatttt atcagtgcgt agtttttctg gaaaattatg atatgaatat
     9781 ggctcgttat ttggtacagg gctgtgacct gtggttgaat actccgcgtc gtggtttgga
     9841 agcctgcggt accagtggaa tgaaagccgt cgctaacggc ggtttacatt tcagtacaat
     9901 tgacggctgg tgggatgaag tctatactcc gggaatcggc tgggcgatcg gaactcgtga
     9961 catttatgag gatattgact attgggatcg tcaagaagct aatattcttt acaatgttct
    10021 ggaaaacgag attattccga gctttttcga acgcgataat gatgacttgc cccgtgattg
    10081 gattgctatg attaaagcat caatgaaagc catctgtcct caatataact ccaatcgtat
    10141 ggtttgtgaa taccttgatc agatttacta tccagccgcc gtgagagcca atgaattgtc
    10201 caaggataac tatgcagtcg ccaaagaatt ggcacactgg aaagagaaaa tgcgtaaaaa
    10261 ttgggacttg gttcgcttcc taaaagttac ctccgggtca atcgaagaat tatcggtaaa
    10321 tagtcagctg gagattcgag cggagatata tttggacggt ttgcaggttg atgatgtgga
    10381 tgttcaggtt tattatggcc gcgttgatcc taacggcgac atcattgacg ggaaattcag
    10441 ttcaatgaaa tttgaaacaa aagttgactc caaaaaatac gtgtacgttg ataaaatcaa
    10501 taattgggaa agcggtttga acggctacac tatccggatt attcccaaac atccggcttt
    10561 agcctatcct ttcgaagatg gattaattca ttggtttgat gaataattga atctaaattc
    10621 taagtgaatg gttataattt taaagtaaat atagatggtt gttgatgaat ttaattacca
    10681 atgaagttgc tcgaaaacgg atcagtcaag gcgaacaagt cgtggtttta actggtgctg
    10741 gcatatcggc acccagcggc atcccaactt ttcgcgggga aaacggttta tggactaaac
    10801 tatctcctca ggaattagcc agttttgaat cgttttattt aaatacagct atggtgagtg
    10861 agtggtataa acatcgccgt gaaattatcg aagtttgtca acccaatgcc ggacattatg
    10921 ctcttgttga attggcgaaa atggtgtcaa aatttacttt agttacccaa aatatagacg
    10981 gtttgcacca gcgagcgggt agccgcgatg tattagaact tcacggaacg attatggaaa
    11041 attactgcat ccgatgcggt cagcaatata gcgccaccga atttgatgaa atttataata
    11101 gttcatttga ccatattcca cgctgttatt gtggcggtct tattcgcccg aacgtagtct
    11161 ggtttggtga atctttaccg aaacaagcct acgaagaggc atatcgtgct tctcaacaag
    11221 ctgacctgtt tctctcagta gggacatctg ctcaggtacg acctgcggct gatctgccat
    11281 taattgctaa agaaaatggt gcctttttag tagaaattaa tattgttgag acggtattga
    11341 ctccttttac cgatttattc ctaaaaggag ccgcggatga aattttgccc caatttaatc
    11401 gggagtacca atatctgctg atgcaggttt gatattaatc tgagtttgcc agaaaaatat
    11461 gaaaaataat atcacggtaa aatctatcga aaccctgttt ttaatagggc tgatattggt
    11521 cttgtcgaat tgtgcctatt acaataccta ttacaatgcc gaaaaatatt ttcgtgaagc
    11581 ggaaaaagaa tttaaaaccc ttcctgatga aaaaatcagc ccggcattga agaaaaaatt
    11641 tgataccgcg atagaaaagg ctaataaggt aatcactcaa tatccggaga gtcgctgggt
    11701 tgatgattcc tactatataa tcgcaatgtc gcattattat aaaggcgatt accgaattgc
    11761 ccgtcagaag ttcgaggatt ttgctgtcaa atttcctcag agcaaattat atcctgaagc
    11821 aatggtctgg tatgggcgta atttgtggaa attggacgaa cgcgagttag cttttttcca
    11881 gtggaaaaaa atcatgaacc ttacggatga ttattatctt ctagcggagc tctatttatc
    11941 aatcggtgaa ttgtatttta aagacagtgc ctatgactcg gctttatttt attatcaaaa
    12001 agcgactgaa gttgggggaa gcttcgatat tgccgccgaa gctcagttcc gtattgccga
    12061 gattgacctg gctttaaaca gaccacaaga agccattaaa aatcttaaaa aaattgacca
    12121 gttttcgcct tctttaatct tacgcgataa gatgcaggtt ctcctggtgc gaatttatcg
    12181 agagtccggt caatatgata aagccattga tttaattaat gaaaaattaa acgatccggc
    12241 taatgagaaa atctggggtg atctggagct acaattggga ttgatttatc ttgctcaaca
    12301 agattacgat gccgcaatct ctcgattgac caaggtaatt gaggattata aaggtaaacc
    12361 ggttgcggcg gaagccagct atcacttagc cgagttgtat atgactcaaa ttcatgatta
    12421 taccaaagcc gctgttcaat atgataatgt tgtcaaaatc gaggttaata ccctgcgggc
    12481 ttttgaatgt cgcaataaat cttctgaaat caaacgattt aatactattt ataatcgtct
    12541 tgccgctcta agtgaacagg ttgccgcgat tgacctgaat ccgccgactg aaaacgatac
    12601 catcggcgag tcgctcccag ttgaccagga gcaatcggag ttgaagaagg cgcttgaaaa
    12661 gaaaaacaca acccagaaga aaacaattga cacgctagca gttttcaaag aatattacac
    12721 taccctttat gaaatcgctg aaatatttta ttttagtttt aatcaacccg atagcgcgat
    12781 ttactatttt gaaaaagttg cctacgaaat cccttttaat tctttacgag ataaagcact
    12841 ctacagtctt taccgaattg ccagcgaccg gcaaaatatc gaaaaggcta acgaatatgc
    12901 taaccagatt aagcagtggt ttccggagtc gatctattta gccgaaattg agaaacggcc
    12961 gcttaatact tccgcccgcg aagtcgaagc cgattcatta ttggtaaaag cagaaaattt
    13021 agaccagact gatctgcatt ctgcgataac aatatacgaa aagcttgcca aggaatatac
    13081 cgattgccaa gccgcggaga aagcggttat gtatttagcc tatttctatc atcaccgatt
    13141 atttgacttg aataatgctt taacctggta caaatattac cttgacactt atcccaatgg
    13201 gcaatattac caaataatgg caaatgctta tgatcaactc aaggaaatag aagatgcctt
    13261 agctcagggg agtgaagccg aggaaaatga gccccaaaag ccggaaaagg ttgaatgaga
    13321 gtattaaaag ataccacggt tttatctaac cggatggtcg ctaccgatac ctatttaatg
    13381 gaatttgcgt ttcccgaaat cagtcagtcg gcgcaacccg gccagtttgt gaacttttat
    13441 tttcctgaac gcccgcaatt gtttccccgt ccttttagca ttgcgggagt gaatcgagaa
    13501 aatctaacca ttctttataa aaaagtcggg tgcctgacct ctattatgag tcaatggcaa
    13561 cccagccaga aaattcgagt tctaggtcct ttaggaaata ccttcaatat caaatataac
    13621 aatcatgtat tgatagccgg tggagtcggt ttggcaccgc tacttttcct gaaccaaaaa
    13681 ttggctaatt tgaattacaa aatctggaat tttgtagggg tgcgttctaa agatgaatta
    13741 ccaccattat cattattatc gggggaaaaa tatatagcga ccgaagacgg gtcagtcgag
    13801 tattgcggta cagtagtaga ttgtctccgg aataatctgg gagcgattcc gcatccattt
    13861 agcgtctatg cctgcggtcc ggaagcgatg ctgagtgctt tatggcattt gtctcgcgaa
    13921 aaccactttg atttacaatt gtcattggaa aaattaatgg cttgcggact cggaatctgc
    13981 cagggatgtg caattcccca ttctcatccg tcggaacaac ctctgcgttt ggtttgtcag
    14041 gatggtccgg tatttaacgc caatgaattg gacttaggat gaccgattta catataaagt
    14101 taagagaaga ttttattctt aggaatccac ttttaacggc ttccggtact tatggttacg
    14161 gcgatgagat agccgatttg attaatccgg agctttatgg cggaattgtg accaaatcaa
    14221 tctcgctcga gcctcgcgtt ggtaatccgc cgccgcgtat tgccgaagtg acctccggaa
    14281 tgcttaattc aatcggcttg gcaaatatcg gagttgataa atttctggaa gagaaggagg
    14341 aatttctggc ggcttataac ggcaaagcaa ttgtcaatat agccggtcgc aaagatgaag
    14401 aatatgttca ggtggttaaa aaattgtccg atcgccaatg gatagcggct tttgaaatca
    14461 acgtttcctg tccgaatgtt agcgagggtg gcattgaatt cggacgagac ccgttaattc
    14521 tgggacaatt ggtcaagaaa ctgcgtcatg ctaccgataa atttctaata attaaacttt
    14581 cgccgaatgt cactgacatc cgggttctgg ctttagccgc tcaggaaaac ggagctgatg
    14641 caatttcgat gattaacacg gtttacgggg cggcgattga tattcataca cgtcgtccga
    14701 agcttaatac tgtagtcggg gggctttccg gaccggcggt caagccgatc gcccttgccc
    14761 atattattaa aacttacaaa gaagttgata ttccaattat cggaattgga ggaatcagtt
    14821 gcggggcgga tgtgattgaa tttttattgg cgggagcgca tgcggttcag attgggacag
    14881 ccaatttttt taatcctaaa gtctgtggcg aaatacttga tttcattgaa caatattgta
    14941 cggagaataa aataagttgt ctgcgagata taatcggacg agtcgaagtc taagcttaat
    15001 cgtcatttta ttactgttta tgatgttggg ctttatgggt tgtgcttccg cgccgcgtta
    15061 cctctatact tcacctaaaa ctggtgcgac aaatgacgat aagaatacgt tactcttgct
    15121 caagaattct aacaggaccg ccgttttgac tgcctcatat tacggagcgg atttccatgg
    15181 taaattaact tccagtgggg aagttttcga tatgaacgga atgacagccg ctcataaatc
    15241 tttgccgttt gggacggttc tgcaagtgac ttatccacca actggtcaat cggttactgt
    15301 ggttgttaat gatcgcggac catttgtgcc cggtcgcgat ttagatttat cgtacgccgc
    15361 cgcccggaaa attgggttgg tcaatgacgg taccggcaag gtgaaagtta aagttcttaa
    15421 atggggaaat aatgattccc cgaaacaatg aaatcaggtt tttttaatga ataacttcgc
    15481 ttctcgtatg ctaatcaata taattggaat tccagccatt ttgctcttga ttcatttagg
    15541 tggaattcct ttcgttcttt ttataacggt aatggttttc atcgcgcagg ttgaacttta
    15601 tcttttgatg cgaaaaaaag gaattgcgcc gctacttttg cctggtgttt tagccggagt
    15661 aatttggcaa cttgccagtt tttatcttcc gggcgcactg ctgctttatt tactgatagg
    15721 attggtagtt ttgctcttac tagctagttt ggtgcgccag atcaaaaacg taacggttga
    15781 tcttgccagt accttgttcg gttttgttta tttaccggtg ttaacttcga ctataatttt
    15841 aatgcgcaat attcctggta gggggcgttc gattgtactt cttttgtttg ttacaatctg
    15901 ggtttgtgat tcactggctt ttataatcgg aaagtggctg ggacgtcgca agatcgcacc
    15961 taaaattagt cctaataaaa cctatgccgg ttgcattggc ggactatttg gtgctgtgct
    16021 gaccgtactc attttttatt ttttaggttg ggcgccggat tttatgagca taatgcaatt
    16081 aattattttt ggtatactgg cgggagtatt cggacaggct ggtgattttg tcgaatcggc
    16141 ttttaagcgg gacgccgaag tcaaggattc atccaattta ttgctcggcc acggtggggt
    16201 gctggatcgc tttgactcat tttttatcgc cgcgccggtt atttttcttt atattattac
    16261 aatattgtag gaaatctaat tgttctcaca catggagata aattgacgga gacgaaaatg
    16321 tcaataaaca atggcaataa cgatctggtt gaatatttac atagtttgag tggtgaaata
    16381 gatccgaaac agggttcggt agctattatc ttagccgccg gtcatggcaa aagaattaaa
    16441 tccgaacgtt ctaaaatgtt acacgagatc tggggtgtgc cgactgtggt gcgcgtcgct
    16501 aatgccgcca gtaaaggctt ggcttctaac aatcagataa ttgtcgtggg tattaaagcc
    16561 tgtgaagtgg cggaatcagc cgggaaaaga gcaaatcgca tttttgtcta tcagaccgaa
    16621 caaaaaggta ccgggcatgc cgtgaaagag gcactttcag ccattagcca gaatggccac
    16681 ataaaagaca tttatatctt cccgggtgat atgggattga ttacaccagc cgtggtcagc
    16741 gaacttaaac gccgttttga acaatccggt tgtggtttag ctttgctaac gggaaattat
    16801 aaaggtgaac cggaagctaa tagctacggt agaattatcc gcgtcccggc aagaacagct
    16861 accggtgagc cctcaggaga agatgaggga cgcgttattg aaattaagga gcataaagat
    16921 attctagcaa ttcccgaaag cggattttat acgactgtgt ataatggtaa aaaatatcaa
    16981 tttaaacgtt cggaattgct gggaatcacg gaatataata ccggcgttta tgcgatgaaa
    17041 tttgacctcg ctcagcgatt tatccatcaa cttactccgg ataatgtgca gggtgaatta
    17101 tacttaaccg atataattgg ccttttcaat gctaataaaa ttgaagtgga agcttatccg
    17161 gttgacgacg aaagtgcagt aatcggtttt aacgtcaaga gtgttttaca ggaaatggaa
    17221 aatatcgccc gccgaaaggt gtggaaactt ctgcgggata taatttatat cgaggatgaa
    17281 aatgattttt tcattgccga tgaggtggtt attcggattt tggaaatgga taaaatcaat
    17341 ccgccgctcg acattgagat cggcaaaggc gtgcatattt cgagtggtgt gcacctcgaa
    17401 cgtggcgtaa aaattcgggc gggctcttta cttaatggta acatccatct gggcgttggc
    17461 acagtcgttc aggaaaatgt ttctcttagc acctatccca atcaacaaat gactattgga
    17521 aaaaattgtg tcattatgcg cggtgatttg ataaaaggta acattacaat cggcgacaat
    17581 acgagcatcg aatcgagcgt cattattacc ggcagtagcg aatatccaac tatcatcggg
    17641 aaaaatgttg tcatcaaagg gacgacttat atatttgggt caattatcga agacgacctg
    17701 tttattgagc attcggtatt gaaatgtaaa agggtagagc gcacagtccg caaagatgga
    17761 acaattcagc cggttcgctg ggttatgccc cagccgcaag gtctggatat cgtccgcgat
    17821 ttgtaacatt aacgtagccg gtaggctggc tagaagaatt catttaggac cgtacgattg
    17881 aatcgttata caaatccgtt ggtctgcgga tagcgtcccg cccaggcggg attgcgatgt
    17941 tcaatatccg acaattccag acatagttta tagggatgat tggaagttcc acatgtcttg
    18001 gttcataagg aaatggaatt ttcgctcctt tcaaataagg ataaattttt acgatttcct
    18061 gcccgccctc ctatgtcggg agcaggcggg tcggcggctt gatctaactt ttttgccgtt
    18121 cttcaagtcg ttgttccatt ttaccttcaa atacttcttt aggtgtcaga tagaacagag
    18181 cgctatgcaa gcgttcctcg ttataaaacc gaatatattc agcgatttgt tcggcaatca
    18241 aactatcctt gtccttaatc ttttcctcta attgtccttt cctggcagat tgcccagtct
    18301 cgctttttca tgctgacgac caaatgtttc aacagcgcct tcaaataact gcttcttt
//
LOCUS       DAOMJU010000038        18132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_2927_length_18132_cov_18.045804, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000038 DAOMJU010000000
VERSION     DAOMJU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 18132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18132
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2927_length_18132_cov_18.045804"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PLO58_05210"
     CDS             <1..879
                     /locus_tag="PLO58_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO74223.1"
                     /translation="ANSTPETIANIHTNIETLNGKIVTIRGIVTIGAGVLASGYTSAY
                     IQDASGRGLNLYEYGKLYSDITRGDELMVVGYVEKYNTTIEVTNFIYKKISSGNELPP
                     AQVVTVAGANSSDWEGTLIEFSGMVTKQETSGEATNISVSAGTDTTIARIPDATGIDV
                     ASIALGNSYKFRGVGNKYKSTYQTLIGYAEDIVPLTAVKESKTNALTFKLNPAYPNPF
                     NPATTISWQLDKNGAYEVVVFNVLGQKIDVIASGYATAGSYAKVWNAAKFPSGVYFVQ
                     LQAGNRVRTQKMLYLK"
     gene            883..2877
                     /locus_tag="PLO58_05215"
     CDS             883..2877
                     /locus_tag="PLO58_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQO74224.1"
                     /translation="MKKRIILPGLILLPVLLLTAQDHVVISEIVLQPSNAEYIKLYNP
                     TDNAVDLSNYYLTDGTDPSNQKYYHNLPTGTNFWSGSGSDFIVRFPPGYSIEAQSEII
                     IAATTTAAYESYYGSSPDLSVKDNMLNAIDGQNTKGIGTAYLDNAAETLVLFYWDGSS
                     ATVKDVDYVLWGSLICAVDKSGVAGYQNDTPVAEQTCAPTHTDGFKLQRISDEGSEPS
                     SGGNGITGHNETGENLDGTWQVVAVGNTKPKLSSINYSPSSPLVGQAITFSVNVTDDG
                     SIAAVRLIYSFQSETGTVEMSLTTGNTYSVTIGPFSIAGILYYQIEAEDESGLISTSN
                     QNMITIQAPPEMLTIKTIRENWDEYNGQTVTLRGVVTIGSNILITSRTSAYFQDVSGR
                     GLNLFASAITNLMQGDSIEVTGELTEYNGVKELTNWTNSYTVLAQNVPITSTAKVYIS
                     ELANNLTDWEGSYVEIHGTVAERSNPTATNQGCNVVIEDETARTTVRIWNTTGALYNS
                     LGEIVNEYTDSLLTLGNQVIVRGVVGIYNNAPQILLGYASDVEPYIVGEVGEGRTKIS
                     VAPYPFVPKLGEVIKYTYEYPANCRAIVRVYDLSGRYITTLVDNYFAVSWKREDTWNG
                     RNELNQLVPPGNYILHLQTTNRSTGKSQISIAPVVVGVKF"
     gene            2877..3794
                     /locus_tag="PLO58_05220"
     CDS             2877..3794
                     /locus_tag="PLO58_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74225.1"
                     /translation="MRTFKHLLIAFLFIIIPIALWAQVFTQVNEIAAPISATGSSNLA
                     NIGSAWAQLNNPAGLCRLTALELVGGLYRPYGLSYYNSAVAIIAMPIGKVGTMAIGYQ
                     GSSTSYSGNSLTSETAFSLSHGFYLQKDLFSTLSLGYTLNLYQLDYGHSAGVSGDGSD
                     GMNLGSGFGFGLDLGLQGSLHERSWIGLFVKNINSPEMGSALSAQRLPRSVAIGLGYE
                     PYYGLTTNFVIYQKIGTDKTQYRAGLEYRIGPWLVLRTGVNTEPNRISFGFGIEKWGI
                     LADYAYISHPLLPETHQFSLGYSFRKNEK"
     gene            3799..6279
                     /locus_tag="PLO58_05225"
     CDS             3799..6279
                     /locus_tag="PLO58_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQO74226.1"
                     /translation="MRHSKYCWLLILFIMALNIIVGAVELESKIDLNTADEAELARLP
                     LTRQQLEDILYWREYIGPFNSIFDLKNIKSIDHETFLKLKELTQISPVFLEESEKRIE
                     DNYYKVEQWISDDGASENTVNNWIDMLSNPVNINKLSYFELRNLAGLAPSDAVAIVNR
                     QKQGEIKNRTDLRSTEGLTYYGFSNLEDFIRYTDDDNVHRFGGSFSSVVKNITLSQTP
                     SDDADSYAAFVQLDQPLDTYYKMRLHWGTKFRLEGSYLRNLGEQPIYYQGTKIPQFKA
                     FFEINNVEIGAVKLNKIVIGDYLAGFGQGVTFEATDFFMPRKTGYGFRNRLNGLSGNS
                     SRITQYGLRGIGAEAQIGNLIGTGFFSINNRDAVVNDDGSFSTLITMYPRMSHGLYGN
                     IPHNMINAVKEMVIGGNAKYSFVPGTYLGLTIYQSLYDRELDLHVTESLLDPSGYSKY
                     LTQIGNSADAEINASYESSAPSSIWSSAKSIRRVYGLEFSTVISNLTLQGEYAELDKD
                     LKLGDRSGDPSALVVSGYLQFDNFNFLMLYRDYDLDFDNPYQRSYSNYQRFKGTIYED
                     VYYLKDPSLAYLYSATAQPQAERGLYLSTRYQLHRSLIATAEQDIWTRVADNAQYYRT
                     VLNLEYRPVFKYRFKVRQKWQHRDKTNTLTPVFYQSNETRLEATMRMSRYNQVKLLYS
                     ISFTEFTPRSRLVINAETGETSYVGNAGSPSDALGLTVTHNVNERLKLIGSVMTYSGF
                     LWNFEDTDFRVFNTDTRALHGWITVFSRLSRDLSLRLKYSFDLHNQLDNVVGGMIDIG
                     ADSSNPVVSEVYYDKFYSDFRIQLDYRF"
     gene            6280..7626
                     /locus_tag="PLO58_05230"
     CDS             6280..7626
                     /locus_tag="PLO58_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74227.1"
                     /translation="MKKQIFILTFVLIVSSTGLSQSFFSPLIGDLPSVADARSLAVGL
                     SGWGDGGSASALLTNSALLGYSKQKLSLIAGLNAFSIKEKRSFPVQDSYGDFLADNTY
                     VLNQNWFPDFQVGINAKPLARIQLAMLLNHTNLSDFQYEEEVRGSLYGEYNRDPLVGY
                     NRLSNRYSVFNTSLGMAVNPWKSLWLGAALNFITPDFSEQKYEIQVIKPSDYQSSDHT
                     ISYVVKPKLESAITGNFGMAFDVTKHLSLAISYRLPYNTVQKGGLLYLVNDTTQTLPV
                     LAIDSVFTIEKVTYHQPGELRFGLCLKPVNIIPTQLFFEIVYQNWKDASAKYQFRSDA
                     NPNDIPNNFSDVPIPYRNVTKIHLGIEHIFFSGMPLRLGFYHDPNPINAEMNRNWFTA
                     GTAYQFGNFNLEISGAFTTCDYKYHDLFPIAVEKRVEFDTVRESYLIGMATLRYNF"
     gene            7630..9267
                     /locus_tag="PLO58_05235"
     CDS             7630..9267
                     /locus_tag="PLO58_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HQO74228.1"
                     /translation="MKYKRSSIILIVVLIAETLLAAEKFPAKDYLKIVTIFTNDMHGG
                     IDRSEASFINPDFPPMLGGGAVAGRYILQKRQEAAQNGWGFLLIDAGDFYQGTPLGTV
                     SEGEAVIEYMNTVGYDALTVGNHDFDNGWKNLKKLAEKANFPFLATNLYRKSTGELAE
                     FVKPYIIKEIQGIKIGIIGATLSATPSMSYPEHIEDLDFRPEIEGIKRYLPELKAQGV
                     NTVIVLTHAWLAYDPEEGYKQMMADLSDGELGESYNTSAQAIAHRIPGIDIMFSGHLH
                     RGFYEPWEDPLNHTLIFQNYANGSNLGHVNFYIHRQTGTLAGYDYESDDGAIFTLFED
                     DFLPDTSLTRLIDFWVQKAEAGFDEVIGLATGQIYRSSSGESPMGNLMADAMLASTKA
                     DIAFSNFGGVRADLPAGQITPRHVFKVMPFGNRIVIMKVTGKFLKELVEDRVSGSNQG
                     MLVGGAEIVIDRNQPDGSRVIKFNIGGKPIEYEREYTLAVTDYLAEGNSGYDLLTTIT
                     PDRTNYTGILLRQAMIDYIREHSPIVPKTDGRWREIR"
     assembly_gap    9275..9374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9375..10996
                     /locus_tag="PLO58_05240"
     CDS             <9375..10996
                     /locus_tag="PLO58_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HQO74229.1"
                     /translation="PLDSEFINYIEMVKKGEVKKFTPEGYALGYIPHPLTFETEIPIH
                     LQKQVSLPERYDLREEGKLTSVKDQKDCGCCWTFATYGSIESRWKALELGDYDLSEQN
                     LRNGHGFLLGSCEGGNAKMATAYFSRGNGPIAEVDDPYNVKDSIYVSGLSSQAYVPDA
                     RFLPDDINILKQIVYTYGAVYTDMFWDNTYYNSQNHTYYSYVDSSINHAVLLVGWDDS
                     KETAGGTGAWIIRNSWGSGFGENGFFYISYNDTKVNTSPAFWPNRMDYNPNATVHYYD
                     KLGCVTSFGWVDNSLDHPDYEDYGLVKFVISTDQKITKVGTWVHSSNSTVNFYIYDNF
                     NGSSLSTLLGSTGDKICDYAGYYTFDLPDSIEVNSGDDIYIKVKYNTPETQYSLPAET
                     FITDYADPIIETGKCWISSTGNNNTWLAIGDNTDYKYDLCVKAYGVSTNTAIVGGDDI
                     IPEQILLYQNQPNPFNPVTTIRYSLSTSQYVVLKVYDVLGNEVATLVNEYKIPGNYAV
                     NFDATDLTSGIYLYQMKAGKNIQTRKLMVLK"
     gene            11260..12282
                     /gene="hydE"
                     /locus_tag="PLO58_05245"
     CDS             11260..12282
                     /gene="hydE"
                     /locus_tag="PLO58_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HQO74230.1"
                     /translation="MQKTEIIEFLQGKDDNWLFNEADRIRRAVFGDEIYLRGIIEFTN
                     YCRQNCYYCGLRRSNRAVQRYRLSREEIIAAVETAVSEKIPTIVLQGGEDPYYSAEWI
                     ADLIRKIIDKYDLAITLSLGEQTPEVLQLWRKAGATRYLLRIETLNRENYGFLRPGKR
                     WEDRLNCLKVLRELDYEVGSGIMVGLPGETLATLADAIIGLTELDLDMIGIGPFLAHP
                     QTPLKDKPNGSIQLTLRTLALVRILNPFANMPSTSAMECAEKGARLAALKIGMNVIMP
                     SITPERVKLLYNIYPGKNADSDASEHSIPAVLEIIREAGFHCSFSKGISPNLIRRHES
                     QNILSK"
     gene            12306..13829
                     /gene="hydG"
                     /locus_tag="PLO58_05250"
     CDS             12306..13829
                     /gene="hydG"
                     /locus_tag="PLO58_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HQO74231.1"
                     /translation="MQNVKVSEWTKKVIKPDEIEKYLIEGKDFIDETVITSQLKSAAN
                     PDKSRVREILAKSLEIERLEPEETATLLNVTDDDLWEEMYLAGLAVKRKVYDNRIVFF
                     APLYCSNLCVNNCAYCGFRYENKEEKRRILTMEEIRKETEAVINEGHKRMIVVFGEHP
                     RSAVDYIAETIRTIYDVKVPVKTGFSAIRRVNVNAAPMSIANLKKLWEVGIGTFQVFQ
                     ETYHKPTYANLHPLNTIKGNYRWRLYALHRAMEAGIDDVAIGALFGLYDWRFEVMGLL
                     YHTIDLERHFGIGPHTISFPRLTPASGSWFSRNSQWRVNDADFKKLVTVLRLSVPYTG
                     LIVTARESAEMRRQVIGVGCTQTDASTRIDIGGYTSALQALAKKEKADQQIDEQQFTL
                     GDTRSLDEVVRELAEQGLITSFCTAGYRCGRTGGKIMNLLKGCVEGKFCKANAVLTFR
                     EYLDDYASPETKTVGERLIQKEIEEIKAMPFFQKGRMLETFEKLYQQVASGERDVYI"
     gene            13816..14130
                     /locus_tag="PLO58_05255"
     CDS             13816..14130
                     /locus_tag="PLO58_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74232.1"
                     /translation="MSISKEILQSAASQTNLPDKLNAIAGKLEKEYGLQVNFCKIYGK
                     RWSYVAGDRNLEFAPQRRQLTSRYGIFYKQNDQINPDKMNEIFTALTQILTEIENDCR
                     TQ"
     gene            14175..15380
                     /gene="hydF"
                     /locus_tag="PLO58_05260"
     CDS             14175..15380
                     /gene="hydF"
                     /locus_tag="PLO58_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HQO74233.1"
                     /translation="MQSTPKSNRLQIGIFGRRNVGKSSLMNALTSQYVSIVSDVAGTT
                     TDPVEKPMELPPLGPVLFIDTAGVDDEGSLGGQRIARTRKILERVDLGIIVAEGKNWN
                     KYEDDLLKELQNRQIPTVVAFNKSDLSQPSENLQAYLKQQNIPFINTIATDGFGVLDL
                     HKILIDTTPADYFNSIGIIGDLVKPGEMAVLVVPIDQEAPKGRLILPQVQTIRDLLDN
                     NAGCIVIKENELTSVLNNLKQPPAIVVTDSQAFLKVAAETPADIPLTSFSILFARYKG
                     DLAEYIAGTRTIDKLKPGDEILIAEACTHHPITDDIGRVKIPRWLNQYVGGQLNFTHY
                     QGHDFPEDLSKYKLVIQCGSCMLNRREVLSRIWYCKQARVPVTNYGLTIAYTLGIFER
                     ATALFNIET"
     gene            15515..16012
                     /locus_tag="PLO58_05265"
     CDS             15515..16012
                     /locus_tag="PLO58_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HQO74234.1"
                     /translation="MKNKWMSVILILLLASSLFSAGKEWYSFNEGIALAKKENKHIVL
                     DFYTDWCGWCKVMDQKTFSVPTVNKYLFDNFVPIRINAENDKEKLVFQGKTYTPRQLT
                     RAFQVTGFPSIAFITPEMEVIEVIPGYIEKDLFLNLLKFVHQECYKTGISFDEFVKNG
                     CQGSK"
     gene            16368..16988
                     /locus_tag="PLO58_05270"
     CDS             16368..16988
                     /locus_tag="PLO58_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74235.1"
                     /translation="MKDSIQKNIANQINNRVIPRWEWRTFGSEFPKAEKFLSSLPYDT
                     IKESEEIYILSKNSNENCKIRNSLMDIKIIEQTNPDGLELWKPVLKASFPLSFEIVRL
                     ILRTLHVRKPLAMRDHYTHHQFLNEIVASNPKLSLVDVKKKRRCYTLEGAEVELSELQ
                     VGNQSVKTIAIESEKPQNVQKVLKLLELDNLPNTNYIKALKKMNRH"
     gene            16990..17463
                     /locus_tag="PLO58_05275"
     CDS             16990..17463
                     /locus_tag="PLO58_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HQO74236.1"
                     /translation="MGIEIERKFLVIANDYRTSGNGVRYRQGYLSTAKERVVRVRTVG
                     QQAFLTIKGINVGAVRPEFEYEIPRQDAEQILENICEKPILDKIRYRVKHSGFIWEVD
                     EFLGDNAGLVIAEIELADENQSFIKPDWVGAEVTGDPRYFNSNLIKNPYKSWNIK"
     gene            17580..17666
                     /locus_tag="PLO58_05280"
     tRNA            17580..17666
                     /locus_tag="PLO58_05280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17614..17616,aa:Ser,seq:tga)
     assembly_gap    17809..17908
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5141 a         3701 c         4043 g         5047 t
ORIGIN      
        1 gccaatagca ctcccgagac aatcgccaat attcatacaa acattgagac tttaaatggc
       61 aaaattgtca cgattcgagg aattgtcacg atcggcgccg gtgttttagc aagtggttat
      121 acttctgcct atattcagga tgcgtctggt cgtgggttga atctttatga gtatggcaaa
      181 ttatattccg atattacgcg cggtgatgaa ttgatggtag tcggttatgt cgagaaatat
      241 aacacgacca ttgaagttac taattttatc tataagaaaa tctccagtgg caacgaattg
      301 ccaccggctc aggtcgtaac ggtcgccggt gctaatagca gtgactggga aggcacactg
      361 atcgagtttt ccggaatggt aaccaaacag gaaacttccg gtgaggcgac caatattagc
      421 gtttcggctg ggactgatac gactatagcc cggattccgg atgctaccgg tattgatgtt
      481 gcatctatcg cactgggcaa ttcttataaa ttcagaggag tcggcaataa atataaaagt
      541 acttatcaaa ccctgattgg ttacgccgaa gatattgttc cattaacggc ggttaaagaa
      601 agcaagacca atgctctgac tttcaaactc aacccggctt atcccaatcc gttcaatccg
      661 gcgaccacga ttagttggca gttggataaa aatggcgctt acgaagttgt cgtgttcaat
      721 gttcttggcc agaaaattga cgtaattgcc agcggttatg ccaccgccgg aagttatgcg
      781 aaagtctgga atgccgccaa gtttccatcc ggtgtgtatt ttgttcaact gcaagccgga
      841 aaccgcgtaa gaacgcaaaa aatgttgtac ttgaagtaac ctatgaaaaa acgaattatt
      901 ctacctgggc tgattctatt gccggtttta ctattaacgg cgcaggatca cgtcgtcatt
      961 tccgagattg tcctccagcc gagtaatgcc gaatacatca aactttacaa tccgacggat
     1021 aatgcagttg acctgagtaa ttattatctt accgacggca ctgatccatc aaaccaaaaa
     1081 tattaccata atctaccaac cggtaccaat ttctggagtg gaagcggttc ggatttcata
     1141 gtacgttttc cgccgggtta tagcattgag gcgcaatcag aaatcattat cgcggctacg
     1201 accaccgcgg cttatgaaag ttattacggc tcttcgcccg acctgtcggt taaagacaat
     1261 atgctaaatg caatcgacgg gcagaatacg aaaggtatcg gtacagctta tttagataat
     1321 gcagcggaaa cgctggtact gttttattgg gatggctcat cggcgacggt caaagatgtt
     1381 gattatgtcc tttggggcag tctaatctgc gccgttgata aaagcggagt tgctggttac
     1441 cagaacgata cgccggttgc cgaacaaacc tgcgcgccga cgcataccga cggctttaag
     1501 ctccagcgca ttagtgatga aggctcagag ccatcatcag gcggcaacgg cattaccggt
     1561 cataacgaaa ccggcgaaaa cctcgacggc acctggcagg tggttgccgt cggtaatacc
     1621 aaaccgaaat tatccagtat caattattcg ccgagtagtc cgcttgtcgg tcaggctata
     1681 actttttcgg tgaatgttac cgacgatgga tcaatcgcgg cggttagatt gatttattcc
     1741 tttcaatcgg agactggtac cgtggaaatg tcgctgacta ccggaaatac ttattctgtt
     1801 accattggtc cattttcaat tgccggtatt ctttattatc agattgaagc cgaagatgaa
     1861 tctggtctaa tcagcacttc taatcagaat atgattacaa ttcaagcgcc accggaaatg
     1921 ctgactatca aaaccatccg cgaaaattgg gacgaatata acggtcaaac cgtaactctg
     1981 cgaggtgtgg tgacaatcgg gtcgaatatt cttattacca gccgaacctc ggcttatttt
     2041 caggatgttt cgggaagagg attgaacctc tttgcttccg caattaccaa cctgatgcag
     2101 ggcgattcca tcgaagtgac cggcgaactg accgaataca atggagtcaa agaattaacc
     2161 aactggacta atagctatac cgttctggct caaaacgtcc cgataacgag tactgccaaa
     2221 gtatatattt cggaattggc gaataatttg actgattggg aaggcagtta tgtcgaaatt
     2281 cacggcactg tagcggaacg cagtaatccg accgccacta atcagggatg taacgttgtt
     2341 attgaagatg aaactgcccg aacgactgtg cgcatctgga atacgactgg agcgctttat
     2401 aattcgctgg gcgaaatcgt caatgaatat accgattcat tattgaccct cggcaatcag
     2461 gtaattgtgc gcggggttgt cggtatttat aataatgcac cgcaaatcct gctgggttac
     2521 gcctcggatg ttgagcctta cattgttggt gaagttggag aaggacgcac caaaatcagt
     2581 gtcgcacctt atccttttgt accgaaatta ggcgaagtca tcaagtacac ctatgaatat
     2641 ccggcgaatt gccgcgccat tgtgcgagtt tatgacctgt caggtcggta catcacgacg
     2701 ctggtggaca actattttgc cgtttcctgg aaaagggaag atacctggaa cgggcgcaat
     2761 gaattgaatc agctggtgcc gcccggtaat tatattttgc atctacaaac gaccaatcgt
     2821 tcgacgggaa aatcccagat ttcaattgcg ccggtagtag taggagtgaa attctgatga
     2881 gaacttttaa gcacctgtta attgcttttt tatttattat aattccgatc gcactctggg
     2941 ctcaggtttt cacccaggtg aacgaaatcg ccgccccaat cagtgccacc ggttcatcca
     3001 atttagcaaa tatcggttca gcctgggcac aactcaacaa tccagccggt ttgtgtcgtt
     3061 taaccgctct cgaattagtc ggcggtttat acagacctta tggactttct tattataata
     3121 gtgccgtggc aattatcgct atgccaatcg gcaaagtcgg cacaatggca attggctatc
     3181 aaggcagttc gacctcttat tccggcaatt ccttgacgag tgaaacggct tttagcctgt
     3241 cgcacggttt ttacctgcaa aaagacctat tttcgacgct atcgctcggc tatactctca
     3301 atctctacca acttgattat ggtcattcag cgggcgtttc cggcgatgga agtgacggaa
     3361 tgaatttggg aagcgggttt ggtttcggac ttgatctggg cctccagggt agcctgcatg
     3421 aaagaagctg gatcggatta tttgttaaga atatcaattc gccggaaatg gggagtgcct
     3481 tgagcgctca gcgccttccc aggagtgtgg caattggtct tggctatgaa ccttattacg
     3541 gattgacgac caattttgtg atttaccaga aaatcggaac ggataagact cagtatcgtg
     3601 ccggattgga ataccgtatt gggccgtggt tagtcctgcg aaccggcgtc aataccgaac
     3661 cgaaccgcat cagctttggc ttcggaatcg agaaatgggg cattctggca gattacgctt
     3721 atatcagtca cccgctatta cccgaaactc atcaattcag tctgggctat tcattcagga
     3781 aaaatgaaaa gtaaatttat gaggcattca aagtattgtt ggctattgat cctatttata
     3841 atggctctga acattattgt tggtgctgtc gaacttgaat cgaaaattga cctcaatacg
     3901 gctgatgaag ctgaactggc gcggttaccg ttaactcgcc agcagttgga agatatttta
     3961 tactggcggg aatatattgg cccatttaat agtatattcg acttaaagaa tatcaaaagt
     4021 attgaccatg aaactttttt gaaactgaaa gaactaaccc aaatctcgcc ggtttttctg
     4081 gaagaatcgg aaaaacgcat cgaagataat tattacaaag tcgagcaatg gatttccgat
     4141 gacggtgcca gtgagaatac cgtcaataac tggattgata tgctctccaa cccagttaat
     4201 atcaacaaac ttagctattt tgaattgcgg aatctggcgg gactagctcc aagcgacgcc
     4261 gtggcgatcg ttaaccgcca aaaacagggc gagattaaaa accgcaccga tttacgcagt
     4321 accgaggggt tgacctatta cggtttttcc aatttggaag attttattcg ctatactgat
     4381 gacgataatg ttcacaggtt tggcggcagt ttttcgagcg tggtcaagaa tattaccctc
     4441 agtcagactc ccagcgatga cgccgattcg tatgccgctt tcgtccagct ggatcagccg
     4501 ctggacactt attacaaaat gcgtttgcac tggggcacga agttccgtct cgaaggttct
     4561 tacttgcgca atttgggaga gcaaccgatt tattatcagg gtaccaaaat tccgcaattc
     4621 aaagcctttt tcgaaataaa taacgttgaa atcggcgcgg taaaattgaa caaaatcgta
     4681 atcggtgatt atttggctgg ttttgggcaa ggggtgactt ttgaagctac cgactttttt
     4741 atgccgcgca aaaccggata cggttttcgc aatcggctga acggtttaag tggcaattct
     4801 tcgcgcatta cgcaatacgg cttgcgtggt attggtgcgg aagcccaaat cggcaatctg
     4861 attggcaccg gatttttctc catcaataat cgagacgcag ttgtcaatga tgatggttcg
     4921 ttttcgactc taattacgat gtatccgcgg atgtcacacg gtttgtacgg caatattccg
     4981 cataatatga tcaatgccgt caaagaaatg gtgatcggcg gcaacgccaa atattctttt
     5041 gtgccgggaa cgtatttagg tttgacaatt taccagagtc tgtatgaccg ggaattagat
     5101 ttgcatgtta ccgagtcact gcttgaccct tccggctata gcaaatattt aacccaaatc
     5161 ggcaattccg ccgatgcgga aattaatgcc tcttacgagt catcggctcc atcttcaatc
     5221 tggtcatctg ccaaatctat ccggcgagtt tacggactgg aattttcgac ggtcattagt
     5281 aatttgaccc tgcaaggtga atatgccgaa ctggataaag acttgaagtt aggcgatcgt
     5341 tccggcgatc cgagtgcgct ggtggtgtct ggttatttac agtttgataa ttttaatttc
     5401 cttatgctct atcgggatta cgacctggac ttcgataatc cttaccagcg cagttatagc
     5461 aattatcagc gcttcaaggg tacgatttac gaggatgtat attatctcaa agaccccagc
     5521 ctcgcctatc tttattcggc tacggctcag cctcaggcag aacgcggatt gtatctatca
     5581 actcgctatc aattgcaccg cagcttaatc gccaccgccg agcaggatat ttggactcgt
     5641 gtagccgata acgcccaata ttaccgcacc gtgcttaatc tggaatatcg tccggttttt
     5701 aagtatcggt ttaaggttcg ccagaaatgg caacatcggg acaagaccaa tactctcacg
     5761 ccggttttct atcagtctaa tgaaactcgc ctcgaagcca ctatgcggat gtcgcgttat
     5821 aaccaggtaa aactgttata ttcaatttcg tttaccgaat ttaccccacg ctcacgactg
     5881 gttattaatg ccgaaaccgg tgaaacttct tatgtcggca atgccggttc gcccagtgat
     5941 gcgcttggtc tgaccgttac tcataatgtc aatgaacgac tcaaactgat tggttcggtg
     6001 atgacctata gcggctttct ctggaatttt gaagacacgg atttccgagt gttcaatacc
     6061 gacacgcgcg cgcttcatgg ctggattaca gttttctcac ggctgtcgcg cgatctttcg
     6121 cttcgcctga aatacagttt cgatctgcat aaccagttag ataatgtagt gggtggaatg
     6181 attgatattg gcgcggacag ttccaatcca gtcgtcagcg aagtatatta tgataaattt
     6241 tattcggatt tcagaatcca acttgattat aggttttaaa tgaaaaagca aatattcatt
     6301 ctgacatttg tgttaatcgt tagctcaact ggcttaagcc agagtttctt tagcccgttg
     6361 attggcgatt taccgtcggt agccgacgcg cggtcgttag cggtcggctt atcaggctgg
     6421 ggtgatgggg gaagcgcttc ggctttactc acaaattcgg ctttgctggg atacagcaaa
     6481 caaaaactgt cgctaatcgc gggattaaat gcattttcca ttaaagaaaa acgcagtttc
     6541 ccggtacagg acagttacgg cgattttctg gctgacaata cttacgtgtt gaaccagaac
     6601 tggtttccag attttcaggt tggaattaac gccaagcctc tggcgcgcat ccagcttgca
     6661 atgctgttaa atcataccaa tctgagtgat ttccagtatg aagaggaagt cagaggctcg
     6721 ttgtacggtg aatacaatcg cgatccgctg gtgggttata accgactatc gaaccgctat
     6781 tcagttttca atacttcgtt gggaatggca gtaaatccat ggaaatcact ctggttagga
     6841 gcggcgttaa attttattac cccggatttt tccgagcaaa aatatgagat tcaggtcatc
     6901 aagcccagcg actaccaatc ttccgatcac acgatttcct acgtggtcaa accgaaactc
     6961 gagtcggcga ttaccggcaa tttcgggatg gcatttgacg ttaccaagca tctcagcctt
     7021 gcgatttctt accgattacc atacaacaca gtccagaaag gcggtttact ttatttagta
     7081 aatgacacta cccagactct gccggtactg gcgattgatt cggtttttac tatcgaaaaa
     7141 gttacctatc atcaaccggg tgaattgcgg tttggattat gtttaaaacc agtaaatata
     7201 attccgacac aattgttttt cgaaatcgtc tatcagaatt ggaaagatgc ctcggctaaa
     7261 tatcagttta ggagtgacgc caatccgaat gatattccga ataatttttc ggacgtgccg
     7321 attccttatc gcaatgtgac gaaaatccat cttggcatcg aacatatttt cttttccggt
     7381 atgcctttgc ggctcggatt ttatcacgat cctaatccga tcaatgccga gatgaatcgc
     7441 aattggttca cggcgggcac cgcttatcaa tttggtaatt tcaatctcga aatttccgga
     7501 gcttttacaa cttgcgatta caagtatcac gacctttttc caattgcggt agaaaagcgg
     7561 gtcgaatttg ataccgttcg cgaaagttat ctgattggaa tggcaacctt gaggtacaat
     7621 ttttaatcta tgaaatacaa aagatcatct attattttaa ttgtcgttct aatagcagag
     7681 acgcttttgg cggctgaaaa atttcccgct aaagattact tgaaaatcgt aacgattttt
     7741 accaatgata tgcacggtgg aattgatcgg agcgaagcca gttttattaa tcccgatttt
     7801 ccgccgatgc tgggtggcgg agcagtcgcg ggacgttata ttttacaaaa gcgtcaggaa
     7861 gccgcccaaa atggctgggg ttttttattg attgatgccg gtgattttta tcaggggacg
     7921 ccgctgggaa cggtttcgga gggcgaagcc gttatcgaat atatgaatac cgttggatat
     7981 gatgccctga ctgtcgggaa tcacgatttc gataatggct ggaaaaatct gaaaaaattg
     8041 gcggagaaag ccaattttcc atttctagcg actaaccttt accgcaaatc aaccggagaa
     8101 ttggctgaat ttgttaagcc gtatatcatc aaagaaattc agggaataaa aatcggcatc
     8161 atcggagcga cgctttctgc gacgcccagt atgagttatc cggaacacat tgaagacctg
     8221 gatttccgcc ctgagattga aggaatcaaa cgctatttac cggaattaaa ggcgcaaggt
     8281 gttaatacgg ttatcgttct gacgcacgcc tggttagcct acgatccgga agaaggctat
     8341 aagcaaatga tggctgatct cagtgacgga gaactgggtg aaagttataa taccagcgcg
     8401 caggctattg ctcatcggat tccggggatt gatattatgt tttctggtca tttgcatcgc
     8461 ggcttttacg agccgtggga agatccgctt aatcataccc tgattttcca aaattatgcc
     8521 aatggcagta atctgggaca tgtgaatttt tatattcatc gccagaccgg aactctggcg
     8581 ggttacgatt atgaatctga cgacggcgcg atattcacct tgtttgaaga tgactttttg
     8641 ccggatacca gtctgacacg actgattgat ttctgggtcc aaaaagccga agccggattc
     8701 gacgaggtca tcggtttagc gaccggacaa atctatcgct cgagtagcgg cgaatcaccg
     8761 atgggcaacc tgatggctga cgccatgctt gccagtacaa aagccgacat tgctttttct
     8821 aattttggcg gagtcagagc cgacctgcct gctgggcaaa ttactcctcg gcatgttttc
     8881 aaagtaatgc cgtttggcaa tcgcatcgtc ataatgaagg taaccggcaa atttttgaaa
     8941 gaattagtcg aagatcgcgt ttcaggtagc aatcaaggta tgcttgtggg cggggctgaa
     9001 atcgtaatcg atcgcaatca acccgatggc tcacgagtta taaaattcaa cattggcgga
     9061 aaacctatcg agtatgaacg cgaatatact ctggctgtta cggattattt ggcggaaggt
     9121 aattctggtt atgacctatt gacgaccatc acgccagatc gcaccaatta caccggcatt
     9181 cttctgcggc aagcgatgat tgattatatt agagaacact ctccaatcgt tccgaaaact
     9241 gatgggcgat ggcgggagat taggtaagat taagnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnctccgc ttgactcgga gtttatcaat tatatagaaa tggtaaaaaa
     9421 gggtgaggta aagaaattta cgcccgaagg atacgccctt ggctatattc cgcatccatt
     9481 gacttttgaa acagaaattc cgatacatct tcagaagcaa gtttcgttac cggaaaggta
     9541 cgatttgcga gaggaaggca agctaacctc ggtaaaagat cagaaagatt gcggttgttg
     9601 ctggacattt gcgacttacg gttcgatcga atcaagatgg aaagctttag aattggggga
     9661 ttatgacctt tctgaacaaa accttagaaa tggccatggc tttcttttag gttcctgcga
     9721 aggcggtaat gccaaaatgg caactgccta tttcagtcgc ggaaatggcc caattgccga
     9781 agttgatgac ccttacaatg taaaagatag tatttatgtt tcgggtttaa gttctcaagc
     9841 ctatgttccc gacgctcgat ttttaccgga tgacataaat atccttaaac agattgtata
     9901 tacttatggc gctgtatata ccgatatgtt ctgggataat acttattata attcacaaaa
     9961 tcatacctat tatagttatg tggatagtag tatcaaccat gcagtcctgc tggtaggatg
    10021 ggatgacagt aaggagactg ctggtggcac aggagcctgg attataagaa atagctgggg
    10081 ttccggtttc ggggaaaatg gctttttcta catttcctat aacgatacca aagtaaatac
    10141 gagcccggct ttctggccaa atcgtatgga ttacaatcca aacgctactg ttcattacta
    10201 cgacaaatta ggatgtgtta catcgtttgg ttgggttgat aactcccttg atcatcccga
    10261 ttatgaggat tatggtctgg taaaatttgt tatatctact gaccagaaaa ttaccaaagt
    10321 agggacttgg gtgcattctt ccaattcgac ggtgaatttt tatatctatg ataattttaa
    10381 tgggagctct ctttcgactc ttttgggttc aaccggagat aaaatctgtg attacgccgg
    10441 ttactatacc tttgatttgc ccgattcgat agaagtaaac agtggtgatg atatttacat
    10501 aaaagtaaaa tacaatactc ctgagaccca atattctctt ccggctgaga cgtttattac
    10561 cgattatgcc gacccaatta tcgaaacagg taaatgctgg attagtagta cagggaataa
    10621 taatacctgg ctcgcaatag gagataatac cgattataaa tatgatctct gcgttaaagc
    10681 ttatggtgtt tcaactaata cagcgattgt gggtggagat gatataatac cagagcaaat
    10741 ccttctttat cagaaccaac cgaatccttt taatccagta acaacaatcc gttattcatt
    10801 atcaacctcg caatatgtcg tattgaaagt ttatgatgtc cttggcaatg aagtggctac
    10861 tttggttaat gaatataaaa tacctggcaa ttatgcagta aatttcgatg caactgattt
    10921 aaccagtggc atttatcttt atcaaatgaa agcgggaaaa aatatccaaa ctcgcaaact
    10981 tatggtactt aaatagtatc aagtgacatt aagacaggta gtattttctc ttgtgaatta
    11041 aaagctggtt tatttttacc attagattaa cctgaattat tctaaataat tttttatagg
    11101 attaacatta tttttgttaa aattgtgaac taaaggttat ttgagtgatg atgtaaaaag
    11161 taagattatg aaactattat tgtggataaa gatatcaaaa gtgtgaaatc taaaattttt
    11221 atttggccat ttctaattgt caatggttac taaataacta tgcaaaaaac cgaaatcata
    11281 gaatttttac aagggaagga tgacaattgg ttattcaatg aagccgatcg cattcggcgg
    11341 gcggttttcg gcgatgaaat ttatcttcgt ggtattatcg agttcactaa ttactgtcgg
    11401 cagaattgct attactgcgg attgcgccgt tccaatcgcg ccgtccagcg ctatcggtta
    11461 tcgcgtgagg aaattatagc cgcagtggaa actgccgtta gtgaaaaaat tccgacgatc
    11521 gtcctgcagg gtggggaaga cccctactat tcagcggaat ggattgccga tttaatccgc
    11581 aaaattattg ataaatatga tctggcgatt accctttcac tcggcgaaca gactccggaa
    11641 gttttacaat tatggcgaaa ggcgggcgcg acccggtatt tgttgcgcat cgagaccttg
    11701 aatcgggaaa attatggctt tttacggcca ggcaaacgat gggaagaccg cctgaattgc
    11761 ctgaaagttt tacgagaact ggattacgaa gtcggtagtg gaattatggt gggattgccc
    11821 ggtgaaactt tggcaacgct ggctgatgcc attatcggat tgactgaact tgatcttgat
    11881 atgatcggga ttggtccgtt tctggctcat cctcagacac ctttgaaaga taagccgaat
    11941 ggttcaatcc aattgactct gcgaacgctg gcgctggtgc gcattctgaa cccctttgct
    12001 aatatgcctt cgacgagtgc tatggaatgt gccgaaaaag gcgctcgtct tgcggctcta
    12061 aaaattggaa tgaatgtcat tatgccgtca ataactccgg agagagtcaa attgctctac
    12121 aacatttatc ccggcaaaaa tgccgatagc gatgcttctg aacattcgat accagccgta
    12181 ttggaaatta tccgtgaggc gggatttcac tgcagtttta gcaagggaat ttcaccgaat
    12241 cttattcggc gtcacgaatc ccaaaatatt ttatctaaat aaatgatttt ttcaatggag
    12301 tataaatgca aaatgtcaaa gtaagtgaat ggacgaaaaa agtgattaag cccgatgaga
    12361 tcgaaaagta tctcatagaa ggcaaggatt ttattgatga aaccgtcatt acatctcaac
    12421 ttaaatccgc ggccaatccg gataaatctc gcgttcgtga gattttagcg aaatctttag
    12481 agattgaacg gctcgaaccg gaagagacgg caacattgct gaatgttact gacgacgatc
    12541 tctgggaaga aatgtactta gccggtctgg cagttaagcg gaaagtttac gataatcgta
    12601 tcgtattttt cgctccgctg tactgcagta atctttgcgt caataattgt gcttattgcg
    12661 gtttccgcta cgaaaataaa gaggagaaac gtcgaatttt aaccatggaa gaaattcgca
    12721 aggaaaccga agccgtgatt aatgaaggtc ataagcgtat gatcgtagtc ttcggcgagc
    12781 acccgcgctc ggctgtggac tacattgccg aaaccattcg cacgatttac gacgtcaaag
    12841 ttccggttaa aaccggcttt agtgcgatcc ggcgcgtcaa cgtcaatgcc gctccgatga
    12901 gtatcgccaa tctgaagaag ctctgggaag taggaatcgg tacattccag gtttttcagg
    12961 aaacctacca caaaccgact tatgcgaatc tgcatccgct taatactatc aaaggtaact
    13021 atcgctggcg tttgtatgct ttacatcgcg caatggaggc tggaatagac gacgtagcca
    13081 ttggcgcgct tttcggactg tatgattggc gcttcgaagt tatgggcttg ctgtatcata
    13141 caattgatct cgagcggcat ttcggcatcg gaccgcatac gatttcattt ccgcgcttga
    13201 cgcctgccag cggttcctgg ttcagtcgta attcacaatg gcgggttaat gacgccgatt
    13261 ttaagaaact ggttaccgtt ttacgcttat ccgtgcctta taccggatta attgtcaccg
    13321 cgcgcgaaag tgctgaaatg cgtcgccaag tcattggggt cggttgcacc cagacggatg
    13381 cttccacgcg aattgacatc ggtggttata cttccgcttt gcaagccctg gcaaaaaaag
    13441 aaaaggctga ccaacagatt gatgaacaac aatttactct cggcgataca cgcagtctcg
    13501 atgaagtcgt ccgtgaattg gcggaacaag gtctgatcac ttctttctgt accgctggct
    13561 atcgttgtgg ccgcactggt ggcaaaatta tgaatttgtt aaaaggttgt gtcgagggaa
    13621 aattctgtaa ggcgaatgcc gtcttgactt tccgtgaata ccttgacgat tacgcctcgc
    13681 ctgaaactaa aacggttggc gaacgattga ttcaaaaaga aatcgaggaa attaaagcga
    13741 tgccgttttt ccagaaaggg cgaatgctgg aaactttcga gaaactctat cagcaagttg
    13801 ccagcggcga acgtgatgtc tatatctaaa gaaattttac aatcagccgc gagccagact
    13861 aacctaccgg ataaactaaa cgcaattgcc ggaaaactgg aaaaagaata tggcttgcaa
    13921 gtcaactttt gtaaaattta tggcaagcgc tggtcgtatg tagccggtga ccggaacctt
    13981 gaatttgctc ctcagcgccg ccaattgact tcgcgatatg gaattttcta taaacaaaac
    14041 gaccaaatta atcctgacaa aatgaacgag atttttacag cactaaccca aatcctgacg
    14101 gaaatcgaaa atgactgcag aacacagtag tccaaaatgt tatttcattt ccaaaatctg
    14161 gcggaattga aaatatgcaa agcaccccca aaagcaatcg tctccaaatc ggaattttcg
    14221 gtcgtcgcaa tgtcggtaag tcgtctttga tgaatgcgct gacaagtcaa tatgtctcaa
    14281 ttgtctccga tgtggctgga actaccaccg atccagtcga aaagccaatg gaattaccgc
    14341 cgcttggtcc ggtgcttttt attgatacgg ctggagtgga cgatgaaggc tccctcggcg
    14401 gacagcgtat tgcgcgcacg cggaaaatat tggaacgagt agatctcggg attatcgttg
    14461 ccgaagggaa aaattggaat aaatatgaag atgatttgct gaaggaattg caaaatcggc
    14521 aaattccgac cgtagttgct ttcaataaat ctgatctatc acagccttct gaaaatctgc
    14581 aggcatattt aaaacagcaa aatattccgt ttattaatac catcgcaact gatggtttcg
    14641 gcgtgctgga tttgcataaa attttgattg atactactcc ggctgattac tttaattcca
    14701 tcgggattat tggggatttg gttaagcctg gtgaaatggc tgtgttagtt gtgccgattg
    14761 atcaggaagc acccaaaggg cgtttaatct taccacaggt tcagacgatt cgtgaccttt
    14821 tggataataa tgctggttgc attgtcatca aagaaaatga attaacctca gtattaaata
    14881 atttaaagca accaccggcg attgtggtga ccgattcgca agcttttctg aaagttgccg
    14941 ccgaaacccc agccgatata ccattaacat ctttttcaat tttattcgcc cgttacaaag
    15001 gcgatttggc agaatatatt gctggaactc gaacgattga caaactcaaa cccggcgatg
    15061 aaattttaat tgctgaagcc tgtacgcatc atcccataac tgacgatatt ggtcgcgtaa
    15121 aaattccccg ctggttaaat cagtatgtcg gtggacaatt gaactttact cactaccagg
    15181 gacacgattt cccagaggat ttaagtaaat ataagctagt gattcaatgt ggtagttgta
    15241 tgctgaatag gagggaagtc ctatcacgca tctggtattg taaacaagcg agggttccgg
    15301 ttacgaatta tggattgacg atcgcctata cgctcggcat attcgaacgg gcgacagcat
    15361 tatttaatat tgagacctaa aaacgctaaa agcgcgagtt gtaccaattt tcttggacaa
    15421 atacttttcc tatatttttt attaatttga ttttataaaa tctatgcaac caataccgca
    15481 attggttatc aaaataacaa aaaatggaga taaaatgaaa aataaatgga tgtcggtaat
    15541 tttgattcta cttttagctt ccagcctatt ttcggcaggg aaagaatggt atagttttaa
    15601 cgagggtatt gctctggcta aaaaagagaa taagcacatc gtactcgact tctacactga
    15661 ctggtgtggc tggtgcaaag ttatggatca aaagactttc tcggtgccga ccgtaaataa
    15721 atatttattt gataattttg ttccaattcg gatcaacgcc gaaaatgaca aggaaaagtt
    15781 ggttttccaa ggtaaaactt ataccccccg tcaattgacc agagcatttc aagtgaccgg
    15841 ttttccatca attgctttta ttaccccaga aatggaagta atcgaagtaa ttcccggcta
    15901 catcgaaaaa gaccttttcc ttaatctatt gaaatttgtc catcaggaat gttataaaac
    15961 tggaatcagt ttcgatgaat tcgtcaaaaa cggatgccag ggttccaaat agtccccatc
    16021 aaactaaaat cgcaaaaatc ttccaaaata tctctctgaa aaaacttaga tttacctcta
    16081 aaatatgttt atcagaacta ttttttggca ggtaaaattt gtaatatcta aatttgattg
    16141 tttatgaatt tggctgaaaa ataggaagta taagtcggtg ataaagtcac tacctggaag
    16201 agtaaaaact tcaaaatcga aaaaagccaa tttatatgcc gatcgccttc aagtgaccgg
    16261 caaaatcata aaatcaggaa taataaatcc aagtctatct ctttttgatt ctcatctatt
    16321 taattacatt aatcaaacaa attgtaagcc aataaccaag aatacctatg aaagattcaa
    16381 ttcagaaaaa tatcgctaac caaattaata acagagtaat tccaagatgg gaatggcgca
    16441 ctttcggatc ggaattcccc aaagcggaaa aatttttgag ttcactccca tacgatacta
    16501 tcaaggagag tgaagaaatt tacattttat caaaaaattc caatgagaat tgcaaaatcc
    16561 gcaattcgtt gatggatatt aagattatcg aacaaaccaa tcccgatgga ttggaattat
    16621 ggaaaccagt tctaaaggct tcatttccac ttagttttga aatcgtacgt ctcatactgc
    16681 gcactttaca tgttcgtaag ccgctggcta tgcgcgatca ttatactcat catcaatttt
    16741 taaatgaaat cgtcgcatcc aatccgaaac taagtttggt agatgttaaa aagaagcgaa
    16801 ggtgttacac tctcgaaggg gctgaagtcg aattatccga attgcaggtt ggcaatcaat
    16861 cagtaaagac cattgccatt gaaagcgaaa aaccgcaaaa tgtgcaaaaa gtactaaaat
    16921 tgctcgaatt ggataacctg cccaatacca attacatcaa agctttgaaa aaaatgaaca
    16981 ggcactaata tgggaataga aatagaaagg aaatttctgg taattgccaa tgattatcgt
    17041 acttctggca acggtgtccg ttatcgtcag ggttatctca gcacagccaa agagcgagtg
    17101 gtacgggttc gaacagtcgg tcaacaggca tttttgacaa ttaagggcat aaacgtcgga
    17161 gccgtgcgac cagagtttga atacgaaatt cctaggcaag atgctgaaca aattctcgaa
    17221 aatatctgcg aaaagcccat tttagacaaa atccggtatc gagttaagca tagcggattt
    17281 atctgggaag tggatgaatt tctgggagat aatgctggat tagtaatcgc tgagattgaa
    17341 ctcgccgacg aaaaccaatc atttattaag ccggactggg tgggagctga ggtgaccggt
    17401 gatccgcgtt acttcaattc caatttgata aaaaatccct acaagtcctg gaatattaaa
    17461 taatcccaaa ataaagtatc ttttaaatta ttgtttggtt tgttggtaaa ataagtggta
    17521 cctatctgaa aatattttga ggaaacttcg ctaaaaatat taaattaacg ccgctatttg
    17581 gagaggtggc agagtggttg aatgcggcgg tcttgaaaac cgtttctcga cttatcggga
    17641 cgggggttca aatccctccc tctccgcaaa tgaacgtggt tgaatgcggc attcttgaga
    17701 accgtttacc gacatctcgg gacgggggtt cttccgtagg aactccgctg gagaaatccc
    17761 tccctctccg caaatgaacg tggttgaatg cggcattctt gagaaccgnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga cgggggttct tccgtaggaa ctccgctgga
    17941 gaaatccctc cctctccgca aatttacgat ttcagctttt ttgagggttg ttgtctcgcc
    18001 gccagcgtaa accaagccca ttgataatct ttcggagttc tttcagggta tattttctgg
    18061 tatacatcct gtaatgtttt ataaaatttt tgttctttgg tcattatatt actccattat
    18121 ctctttcatt at
//
LOCUS       DAOMJU010000039        17198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_3192_length_17198_cov_21.544712, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000039 DAOMJU010000000
VERSION     DAOMJU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 17198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17198
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3192_length_17198_cov_21.544712"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="PLO58_05285"
     CDS             complement(<1..1217)
                     /locus_tag="PLO58_05285"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HQO74237.1"
                     /translation="MFHSWRLRRDSHGIAFFFLALGCIIWAFCNIMEYGFNTLSTKTF
                     WVSIEYIGIEMVIVAWFIVIANFTGQTKFTTRRSILLLCIIPIITVFLIWTNRYHGLM
                     RRNITLDTSGPFSIIKKSYGIWFWVTFAYSNLLMISSSIMLMQRLIQPPKLRRSQILA
                     LMACALFPWVGNFLYVFNLLPAPWSRLDMTSTLLSVSGAIMAFGLFRLQIMEIVPIAR
                     DCILENLTDAILVIDKRNRVADYNPACRNMLKLNEIIIGQPVEEVLNPLAIRPELIAE
                     IKNVQEEISFTFGNETKIYDLRILSLFKKKQKLCGRIFHFRDVTDKRLAEMHLEKTVV
                     DLRRALTEVRTLSGLLPICSSCKKIRDDTGYWKDVETYISQHSTAEFSHGICPDCMQK
                     LYPEQYETLVKKG"
     gene            complement(1429..3060)
                     /locus_tag="PLO58_05290"
     CDS             complement(1429..3060)
                     /locus_tag="PLO58_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74238.1"
                     /translation="MFVNFNSVTTIPAIPDWWFWLWPALMYAEMHYRLKFLRGRLFKR
                     QPEILFDVPHRIQTTRLPVVLIIKDAHWFPVTLKQVTLKITSPFDDNLCVEQVFSENL
                     KVDEPWFTREYSLEVAKFRNHLLSVNCTAILDCDGRIITIQNDNYAGLSQADFSVYVD
                     SEPLPADSGWIWGDLHCHSQWTSDQVEFGVPLAVLPDLAKAMGLSFCGLVEHSYDLDD
                     LPDSWTKNDPELRQWFAYRQAVQATNAKLNDFIIIPGEEVSVDNGLGRNVHLAVLNNS
                     EFFPGSGDGLERSVGFASELTYQRVLERAESQALIFAAHPLCKPPLTQQIIARRGIWN
                     LHDQFQRLNGWQILNGQEGKEFSASRNFWIKKLLSGNHSYIYAGNDSHGNFNRFRQVK
                     IPLLLMHENDHQIFGEFLTGVQTDLATGLDDLVANLKNGAVIVSNGPFLNIKAIDSRQ
                     NRYAIGQTMPELPQSVEITALSTRHCGAIHSIRLYIGNFERQVEEIKTVEVAGGIYRN
                     IINLPVSNLPNSGYFRAEVETKNRKFALTNPIWFS"
     gene            3213..3773
                     /gene="efp"
                     /locus_tag="PLO58_05295"
     CDS             3213..3773
                     /gene="efp"
                     /locus_tag="PLO58_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQO74239.1"
                     /translation="MATTADIKNGLVINFNGDLVKVIEFQHIKLGRGGARVWTKFKKI
                     RTGQVIENTFRSGEKIDIVRLEAVEMQFLYRDGSDYVLMNTETFEQITVPEEIFKDAA
                     QFIKENELVKVTFFDGKPIDVEVPIFVNLKVVKTEPGMKGDTVTGASKPAVLETGYTV
                     QVPLFINAGDIIRIDTRSGQYCERVK"
     gene            3789..4271
                     /gene="accB"
                     /locus_tag="PLO58_05300"
     CDS             3789..4271
                     /gene="accB"
                     /locus_tag="PLO58_05300"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HQO74240.1"
                     /translation="MRKEKILELVKILESHEIDEIEISRWGQKIRISKNSNTGISTGV
                     EVAPAPQKAKKGHQPQLAETITFESRTAVTEEINHKGVEVKAPIVGTFYRSPAPDAEP
                     YVNVGDTVAVGQPLCIIEAMKIMNVIEAEVAGKVTKILVENAQPVEYNQTLFIIEKTE
                     "
     gene            4293..>4549
                     /locus_tag="PLO58_05305"
     CDS             4293..>4549
                     /locus_tag="PLO58_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQO74241.1"
                     /translation="MIKKLLIANRGEIALRIIRACKELNIPTVAVYSTADALSLHVRF
                     ADEAVCIGPPESKNSYLKMANIISAAEVTNSDAIHPGYGFLA"
     assembly_gap    4550..4649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4650..4988
                     /locus_tag="PLO58_05310"
     CDS             <4650..4988
                     /locus_tag="PLO58_05310"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053204.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQO74242.1"
                     /translation="IECRINAEDPTKNFRPAPGKIQAFHAPGGPGTRMDTHVYAGYEI
                     PSYYDSLLGKLITHGKDRDEAIVRMERALDETIIEGVPTTIPFHKAIIRNEAFRKGDF
                     NINFLNNFSF"
     gene            5005..5382
                     /gene="gcvH"
                     /locus_tag="PLO58_05315"
     CDS             5005..5382
                     /gene="gcvH"
                     /locus_tag="PLO58_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQO74243.1"
                     /translation="MKVPANLKYTKDHEWARIEGAVAVVGITDYAQSQLGDVVYIDLP
                     KVGDVVARGDVIGTIEAVKTVADIYSPVSGEIVAVNSALKDASELVNKDPYGEGWIAS
                     IKLSNSMELEALLDPADYEKLIS"
     gene            5386..6726
                     /gene="gcvPA"
                     /locus_tag="PLO58_05320"
     CDS             5386..6726
                     /gene="gcvPA"
                     /locus_tag="PLO58_05320"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HQO74244.1"
                     /translation="MHFIPNTCDTERAILENLGVASFNDLIANIPEKFREQCRINLPP
                     ALSEIELTKTMQELSEKNGNSDKQVSFLGGGSYDHFIPAVVDFLISRSEFYTAYTPYQ
                     AEVSQGTLQAIYEYQSQICHLTDMEVANASMYDGATALAEAAIMAANITQRKKFLVSP
                     LINPLARQVLETYLTARDFILADLPEKNGQTDLSVLKEMIDNQTAAVLVQSPNFFGII
                     EDLNYIAEIIHAKSSLLIMSVDPISLAILKTPGELGADIAVAEGQPLGIHQSFGGPYL
                     GVFTTRRQYVRNMPGRIVGQTTDVEGKRGYVLVLQTREQHIRREKATSNICTNQGLMA
                     LAATIYLSLMGKTGLRKVAELCLNNSHYLADKIAGLPGYSLPFAGQPFFKEFVVKTPR
                     PATEIAAAALATGFFAGIDLGQYRREWENYLLIAVTEKRNLQEISDFVKILAKF"
     gene            6732..7484
                     /locus_tag="PLO58_05325"
     CDS             6732..7484
                     /locus_tag="PLO58_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HQO74245.1"
                     /translation="MGVLNQLLEKARLNGGGYFVLIDPDTRSTPELKAFVREICACGV
                     DAILVGGSLIIGGGFHETLDIVKTESDVPVIIFPGSVAQVSNRADAVLYISLISGRNP
                     NALFGEHVIAAPVIRKLGIEPISTGYMLFESGKTTTAEFMSNTRPLPINKPEIAMAHA
                     LAAQYLGMSTVYLEAGSGAERSIPNQLVKAVCDYIDIPVFVGGGIRTPDEARAKVEAG
                     ATFIVTGNVFDNDANIGLAKEFVRAVHVKEHF"
     gene            7497..8072
                     /locus_tag="PLO58_05330"
     CDS             7497..8072
                     /locus_tag="PLO58_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HQO74246.1"
                     /translation="MHQKIIAQLEESAEVKRQTIVTAVDSIEAAAQMLIDCYRAGGKV
                     LLCGNGGSAADAQHLAAELISRLKLERAAIPALALTTNTSLLTAIGNDYKYDLVFVRQ
                     VEAFGRAGDVLIAISTSGESENVIKAVEFSRVQGIKSIALTGAKGGRLAEKVDLAIKI
                     PSDNVQRIQECHITVGHILCELIESALYGTI"
     gene            8088..>8355
                     /locus_tag="PLO58_05335"
     CDS             8088..>8355
                     /locus_tag="PLO58_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQO74247.1"
                     /translation="MISEIQDFINYLKVERNLAPNTIQAYSTDLERYSQFLTQQQIND
                     ISKVRFSDIQNYINVLAELGLAARSLARNYSSIRAFHRFLFSERI"
     assembly_gap    8356..8455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8456..8726
                     /locus_tag="PLO58_05340"
     CDS             <8456..8726
                     /locus_tag="PLO58_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQO74248.1"
                     /translation="LFLSMRGAPLTRMAVWNIIQEYVRLADIRKPISPHVFRHSFATH
                     LLEGGANLRAVQEMLGHADIATTQIYTHLDREFLIEQHRTFHPRY"
     gene            8726..9361
                     /locus_tag="PLO58_05345"
     CDS             8726..9361
                     /locus_tag="PLO58_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQO74249.1"
                     /translation="MNFQVIILLIPPILLALTFHEYMHGWTAYKMGDPTAKMAGRLTM
                     NPIAHLDPIGTLMLFLVHFGWARPVPVDPRYFRNPKRDMLIVAAAGPLANMFIALLSG
                     LLARLFQGGYFSFLPLSVLQPLYVMVILSLQINLALAIFNLLPIPPLDGSKILMGLVP
                     PRYEAQFNWLERYGSFILMAVIIVGMVTNVSILGAFINPFVNFFSQLFGGI"
     gene            9601..>10639
                     /gene="ftcD"
                     /locus_tag="PLO58_05350"
     CDS             9601..>10639
                     /gene="ftcD"
                     /locus_tag="PLO58_05350"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HQO74250.1"
                     /translation="MTEIIECVPNFSEGRDHDVIRAIAVAIEKVKDVKLLDVNSGASV
                     NRTVITFVGNRESVINGAFAGIARAAELIDMRHHRGTHPRLGATDVCPLVPVSGISVE
                     ECIALSHQLAKRVAEELHIPVYLYAKSAQKPERIDLANIRAGEYEGLKQKLSDPNWQP
                     DYGQPIFNPKSGATIIGVRNFLIAFNVNLDTRDCQLAKDIALTIREKGRIKRDQNGNK
                     MYNSQGEPIYETGLLKNVKAVGWYIDEYKMAQVSTNLTNYKVTPPHVVFEEIRRLANA
                     RGLRVTGSELVGLIPKEALLMAGRYYLKKQGYSEDAPESELIQMAVQSLGLNAIAPFD
                     PAKKIIEYRLAE"
     assembly_gap    10640..10739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10808..12625
                     /gene="mutL"
                     /locus_tag="PLO58_05355"
     CDS             10808..12625
                     /gene="mutL"
                     /locus_tag="PLO58_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQO74251.1"
                     /translation="MSKIKILSENLTNKIAAGEVVQRPASIVKELFENSIDAGADEII
                     VVIRNGGKTLCEVVDNGEGMNRDDLLLAFERYATSKIATVEDLTNIHTLGFRGEALAS
                     IAAVAIVNAISTLRGANEGYELRIEGGTFRDIRPTSPKPGTTISVRNLFYNIPARRKF
                     LKSAEVEFRHIVDVMQEFALIHHDLRFIFIHNDREIYNLSPSSLAERIANLFAPEYSQ
                     NLLPVESVADDLKVTGYIGNLNLVRARRGEQFLYINRRFVNDRLMNHAIISGYGGLLA
                     RSEFPFYCLNLELPPEQIDVNVHPTKMEVKFREQDRVYQFLKQATANSLRSITNVIPD
                     LGTFAPHHYYTTPPPPKGTQIQIPAPDQSPSKQSEFDSSRKTFDVPEVEIPPTPFAAG
                     DSTKWQERARRFVEQGETSEADDFRPSVAVYQVHNKYIISQVKSGLVIIDQHVAHERI
                     LFEEAQESMRGENWKAQQLLFPVVVELTVNDFSTLLEILPFLEKLGFRIREFGKQTVA
                     VEAVPAGLRWGNENIIIREILDHYQEFGTKDTSIQDKVAASYACKAAIKSGDPLTTEE
                     MQNLVDRLFATSNPYFCPHGRPIIVNLSLKELDKRFERA"
     gene            12629..>13188
                     /gene="miaA"
                     /locus_tag="PLO58_05360"
     CDS             12629..>13188
                     /gene="miaA"
                     /locus_tag="PLO58_05360"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQO74252.1"
                     /translation="MSANSNSINEALSRKIPFIVGPTAVGKTATAIELAKQLNGEIIS
                     VDSRQVYRGLDVGTAKPTLRQQQEIPHHLVDILDLDEQISAGAYRNLALKTVSEIQSR
                     GRLPIFVGGSGLYVNAVLKGIFQESTTNPEIRREIRQELQEKGVVALYNQLLEIDPDT
                     VLKIHINDVKRITRALEIYRITGQPP"
     assembly_gap    13189..13288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13289..15708
                     /locus_tag="PLO58_05365"
     CDS             <13289..15708
                     /locus_tag="PLO58_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HQO74253.1"
                     /translation="NSDSADNPMKLWYQQPAKQWVEALPIGNGRLGAMIFGDPRLEEI
                     QLNESTIWGGQPHRNDNLEAKEALPKVRQLIFNGKYKEAQELADQKFISKNSQGMPYQ
                     TAGSLYLSFPEHKNFTNYYRELNIETAVVTTRYEVDGVTYKREVFASFPDQVIICRIS
                     TSKPGKINFTASMNHPGTVNIFTEGNDILIMSGITSDCDSIKGEVKFQVQVKVTTESG
                     SIEASQNSLTVNNADIATIYISMASSFKNYSDISENAGEKAQSYLRNALRKNYKQALE
                     NHIADYQRYFKRVSINLGVTDSIKNPTDVRIERFAKGNDPQLVALCFQFGRYLLISSS
                     RPGTQPANLQGIWNNQLYPPWDSKYTVNINTEMNYWLSEPTNLSEMNEPLVQMIEELS
                     ETGRQTAESMYGAEGWVLHHNTDIWRICGPVDGAFWGMWPMGSAWLCQHLFEKYEYSG
                     DREYLKSVYPAMRGAVEFYLDFLVEEPQYKWLVVCPSVSPENAPSCHPEFSISAGTTM
                     DNQLLFDLFTKTIKVAEILNTDKEFVIKIKETLHQLPPMQIGRWGQLQEWMQDWDDPE
                     DHHRHVSHLYGLYPSNQISPYRTPELFSAAKTSLLARGDESTGWSMGWKVNLWARLLD
                     GDHALKLITNQLSPSIQPDGSQKGGTYPNLFDAHPPFQIDGNFGFTAGVVEMIVQSYD
                     GFIFPLPALPEIWKSGSITGIRLRGGFEIVRLEWQNGKISTLTIKSNLGGNCRIRSYD
                     KLIPMGNFKLEEAKGENPNPFYAISQVKEPLISPDANIIPDELRKSHLYDFNTKAGKE
                     YTLILLE"
     gene            15861..17060
                     /locus_tag="PLO58_05370"
     CDS             15861..17060
                     /locus_tag="PLO58_05370"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQO74254.1"
                     /translation="MSNNGILLRILHICSVLIILIQSSVISQVILDPDAVVEEIVSGI
                     QQPEGPVWREGLGLLFSDIKGNKIYKWTSEKGKEIYLDPSYNTNGLAYDLEGRLVAGQ
                     MGLRRVVRFEKDGSQTSLADSYDGKKLNSPNDLVIKSDGAIFFTDPDFNIPSGESKEL
                     NFNGIYRISPSGVLQLLTSSLTLPNGICFSPDESKLYVNDSQVHKIYVWDVVDDSTIT
                     NKKLLYTIPVPFGYADGMKIDEDGNIYCTCSSYVWVISPEGELIGKIDLPTSVSASNC
                     AWGDDDNRTLFITAGNSVYRVRPFVTSINKEDSSLPKSFGLSQNYPNPFNPTTKITYS
                     IPVTSNVMITVYDVSGKEVVTIEDCQKKAGTYTLLFNAEKLASGVYYYNLTANEFSST
                     KKFVILK"
BASE COUNT         4857 a         3488 c         3804 g         4649 t
ORIGIN      
        1 ttaccctttt ttaccaaggt ttcatactgt tccgggtaaa gtttctgcat acaatctggg
       61 caaattccgt gactgaactc agccgttgaa tgttggctaa tataggtttc tacatctttc
      121 caatagccgg tgtcgtcgcg gattttttta caggacgaac agatcggaag caagccagat
      181 agggttctga cttcggttag cgcccggcgt aagtcaacga cggttttttc caaatgcatt
      241 tcagctagtc gcttgtcggt aacgtcgcga aaatggaata tacgaccaca tagtttttgc
      301 ttctttttga aaagcgatag gatgcgcaga tcgtagatct tggtttcatt gccaaaggta
      361 aaagaaattt cttcctgaac gttttttatt tcagcaatta attcgggccg aatagctaat
      421 ggatttagga cttcttcaac cggttgcccg attatgattt cgtttaactt taacatattc
      481 cgacaagccg gattgtaatc ggctacccgg ttgcgcttgt ctatgaccaa aatagcgtca
      541 gttaagttct ccagaatgca atcacgggcg attgggacaa tttccattat ttgtaatcga
      601 aatagcccga aagccattat agcccctgaa actgatagta aagtcgaggt catatcaagt
      661 cgcgaccagg gtgctggcaa tagattgaaa acataaagaa aattgccgac ccatggaaat
      721 aaggcgcaag ccatcagcgc caggatttgc gaacgacgca gtttgggcgg ttggatcaga
      781 cgttgcatca gcattatcga gctggaaatc atcaacaaat tgctataggc gaaagtaacc
      841 cagaaccaaa tgccatagga tttttttatt atagaaaatg gaccgctggt gtccagagtg
      901 atattccggc gcattaagcc gtgataacga tttgtccata ttagaaatac agtgataatc
      961 ggaattatac ataataaaag aattgaccga cgggtcgtaa acttagtttg accggtaaag
     1021 ttggctatta caatgaacca ggctacgata accatttcaa tgccgatata ttcgattgat
     1081 acccaaaacg tcttggtgct tagggtatta aaaccgtatt ccattatatt gcaaaatgcc
     1141 catataatac accctaaagc caaaaagaaa aacgcaatac catgactatc acggcgaagc
     1201 cgccacgaat ggaacattat tactgcacag ataataatgg acaaaatcag aggcacataa
     1261 taaatgctaa aggtaaaatt cataagattc tcgctgattt aaaagatttt gcttccaggt
     1321 gaatttgaaa acgcctatgc acttttaatg tggctcttaa aatcactgga gatattaaac
     1381 tgttcaaatt gataaaactt tttcgttatt ccaatttcgc ttaaagtgct agctgaacca
     1441 gattggatta gttagagcga attttctgtt tttagtttca acttccgccc gaaaataacc
     1501 gctgtttggt agatttgaca ccggtaagtt tattatattt ctataaatac cgccagcaac
     1561 ttctacggtc ttaatttctt ccacttggcg ctcaaaatta ccgatgtaca atcggataga
     1621 atggatggct ccacaatgac gagttgatag agcggtgatt tcaacgctct gcggtaattc
     1681 tggcatagtt tgtccgatcg catatcggtt ttgccgcgaa tcgatagcct taatattcag
     1741 aaaaggacca ttggagacga ttacggctcc gtttttcaaa ttggcgacca gatcgtcgag
     1801 tccggtggca agatcggttt gaacgccagt taagaactca ccaaatatct ggtgatcgtt
     1861 ttcgtgcatt aaaagcagtg gaattttcac ttgtcgaaag cgattgaagt tgccatggga
     1921 gtcattgcca gcatagatat aactatgatt gccggaaagc aattttttta tccagaaatt
     1981 acgactggcg ctaaattcct ttccctcctg accattcaga atttgccaac cattcaggcg
     2041 ttggaactgg tcgtggagat tccaaatacc gcgccgggca atgatttgtt gagtgagagg
     2101 cggtttacag agcgggtgag ccgcaaaaat taacgcttga gattcggctc gctcgagtac
     2161 ccgctgataa gtcagttccg aggcgaaacc gactgaacgc tccagtccat cacccgagcc
     2221 ggggaaaaat tcagagttat ttaagactgc caggtggaca ttgcgtccca agccattatc
     2281 tactgagact tcttcgccgg gaatgatgat gaagtcatta agtttggcat tggtggcttg
     2341 aacggcttgg cgataagcga accattgacg taattcggga tcattttttg tccaggaatc
     2401 cggtaaatcg tccagatcat aagaatgctc aaccaaccca cagaacgata gccccattgc
     2461 ttttgccaga tcaggcagta cagctaatgg tacaccaaat tcaacctggt cggaagtcca
     2521 ttgtgaatga caatgcaaat cgccccaaat ccagccggaa tcagctggca atggttctga
     2581 atcaacataa acgctgaaat ccgcttgact gagaccggcg tagttatcgt tttgaatagt
     2641 aataattcgg ccgtcgcaat ccaaaattgc cgtacaatta acgctcaata gatgatttcg
     2701 gaatttcgct acctccagac tatattcgcg cgtaaaccac ggttcatcta ctttgagatt
     2761 ttcactaaaa acttgctcaa cgcataagtt atcgtcgaaa ggagaggtaa ttttgagggt
     2821 aacctgtttc aaagtaaccg gaaaccaatg agcgtctttg attattagaa cgaccggcag
     2881 tcgagtagtt tgtatccgat gaggtacatc aaagaggatt tccggttgtc ttttaaataa
     2941 ccttccccgt agaaatttta accggtaatg catttcggca tacattagtg ccggccagag
     3001 ccagaaccac caatccggta ttgctggaat cgtagtaacc gaattgaaat tgacaaacat
     3061 agtggtaaaa tctatgattt attcgtattt taatgaagaa aaattccgtt gagaaaagtt
     3121 tgaatagtct atattaattg gaaaaatctt tgaaaataat tcagcgtgcc tgtaaattcc
     3181 ttatcacttt tgatttttaa aatggagtac gcttggctac aacagcagac atcaagaatg
     3241 gcttggtgat aaattttaac ggcgatctgg taaaggttat tgaatttcag catattaaac
     3301 tgggacgagg tggtgcgcgg gtctggacca aattcaaaaa aatccgtacc ggacaagtta
     3361 ttgaaaacac ctttcgctcc ggcgagaaaa ttgacattgt gcgtttagaa gccgtcgaaa
     3421 tgcagtttct atatcgcgac ggttcagact atgttttgat gaataccgaa acattcgagc
     3481 agattaccgt gcccgaggag atattcaaag atgccgccca atttatcaag gaaaacgaat
     3541 tggtcaaggt gacttttttt gatgggaaac cgattgacgt cgaagtgccg atttttgtta
     3601 atctcaaggt tgtgaaaacc gaaccgggca tgaaaggcga cacagttacc ggcgcctcta
     3661 agccagccgt tctcgaaacg ggctatacag tacaggttcc actgtttatc aatgccggag
     3721 atattattcg gattgataca cggagcggtc agtactgcga acgcgttaaa taaaaacccg
     3781 gaggggtaat gaggaaagaa aaaattctcg aattagtgaa gatcctcgaa tcgcacgaaa
     3841 tcgacgaaat cgaaatttcg cgttggggtc agaaaatccg cattagtaaa aatagtaata
     3901 ccggaatatc taccggagtg gaagtagctc cggcgcccca gaaagctaaa aagggtcatc
     3961 agccacagtt ggcggaaaca atcacttttg aatctcggac ggctgttacg gaagaaatta
     4021 accacaaggg ggttgaggtt aaagcgccta tagtcgggac tttttatcgt tcaccagcgc
     4081 cggatgcgga accgtatgta aatgtaggag ataccgtggc tgttggacaa ccgctttgca
     4141 ttattgaggc tatgaaaata atgaatgtga ttgaagccga agtcgccgga aaagtgacca
     4201 agattctcgt tgaaaatgcc cagccggttg aatataatca aacccttttt ataatcgaaa
     4261 aaaccgaatg acatttttac gatagaattt taatgattaa aaaacttctg attgccaatc
     4321 ggggcgaaat agctttaaga ataatccggg cttgtaagga attgaatatc ccgacggtgg
     4381 cagtatattc gaccgcggac gctctttctc tgcacgtgcg atttgccgat gaagcggttt
     4441 gcatcgggcc gcctgaaagt aagaatagct atttaaaaat ggctaacatc atcagtgccg
     4501 cggaagttac taactccgat gcgattcatc cgggatatgg ttttctggcn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tcgaatgccg cataaatgcc gaagacccaa
     4681 ctaagaattt taggccagcg ccgggaaaaa ttcaggcttt ccatgcgccc ggtggccccg
     4741 gaacgcgaat ggatactcac gtttatgccg gttacgaaat tccttcctat tatgattctc
     4801 ttttaggtaa attaattacg catggaaaag atcgggatga ggcaattgtc agaatggaac
     4861 gcgccttaga tgaaacaatt atagaaggcg ttcccaccac cattcctttc cataaggcta
     4921 taatcaggaa tgaggctttt cgaaagggcg attttaatat taatttttta aataattttt
     4981 cgttttaata aggagaatat ccgcatgaaa gtaccagcca acttgaaata tacgaaagat
     5041 catgaatggg ctagaatcga gggcgcggtt gcggtcgtcg gtattaccga ctacgctcaa
     5101 agccaattgg gtgatgtagt ttatattgat ttgccgaaag tcggcgatgt ggtggcgcgc
     5161 ggcgatgtaa tcggaacgat cgaagcggtt aagaccgttg ctgatattta ttcgccggtt
     5221 agtggtgaaa tcgttgccgt taattcagcg ctaaaggatg cttccgaact cgtcaataaa
     5281 gatccttacg gcgaagggtg gattgccagt attaaattat ctaattcgat ggaattggaa
     5341 gcattactcg acccggcgga ttacgagaaa ttaatcagtt agattatgca cttcataccg
     5401 aatacctgcg atacggaacg cgcaatcttg gagaatctgg gagtcgccag ttttaacgac
     5461 ttgattgcca atattccgga aaaattcagg gagcaatgcc ggatcaattt accgcccgct
     5521 ctttcggaaa ttgagctaac taagactatg caggaactgt ctgagaaaaa tggtaacagt
     5581 gataagcaag tgtcctttct cggcggcggc tcttatgatc acttcatccc ggccgtggtt
     5641 gattttttaa tttcgcggtc ggaattttat acagcctata ctccttatca agctgaagtc
     5701 agccagggca cactccaggc tatttatgaa taccagtcac aaatttgtca tctgaccgat
     5761 atggaggtcg ccaacgcctc gatgtatgac ggtgccacgg ctttggcaga agcggctatt
     5821 atggcggcaa atattaccca acggaaaaaa tttctggttt cgccgctgat taatccgctt
     5881 gctcgtcagg ttctggaaac atatttgact gctcgtgatt tcatccttgc cgacttgccg
     5941 gaaaaaaatg gccaaacaga tttatccgtt ctgaaagaaa tgattgataa tcagacggcg
     6001 gccgttttag tgcaatcgcc caattttttt ggaattattg aagatttaaa ctatattgcc
     6061 gaaattattc atgctaaaag ttctttatta ataatgtccg ttgatccgat ttcattagcc
     6121 atcctaaaaa cacccgggga actcggtgcg gatattgccg tagccgaagg tcagccgctc
     6181 gggattcatc aatcgtttgg tggtccttat ctcggtgttt tcaccacgcg acggcaatat
     6241 gttcgtaata tgcccggccg aatcgttggt cagacgacag atgtcgaagg gaagcgcggt
     6301 tatgtattgg ttttgcagac acgcgagcaa catattcggc gggaaaaagc cacttcgaat
     6361 atttgtacta accagggttt aatggcacta gcggctacca tctatttatc tcttatgggt
     6421 aaaaccggat tgcggaaagt agctgaacta tgccttaata atagccatta tctggcagat
     6481 aaaattgccg gactgcccgg ttattctttg ccctttgccg gtcagccgtt ttttaaagag
     6541 tttgtcgtaa aaacgccccg cccggctacc gaaatcgcgg cggcggcgct tgcaaccggt
     6601 ttttttgcgg ggattgatct cggtcagtat cgtcgtgaat gggaaaatta tttgttgatc
     6661 gccgtcacag aaaaacgtaa tttgcaagaa attagtgatt ttgtaaaaat tttggctaaa
     6721 ttttaggtac aatgggagtc ttaaatcaat tactcgaaaa agcccgcctg aatggcggtg
     6781 gatattttgt attgattgat cctgacacac gctcgacgcc ggaattaaaa gcgttcgtgc
     6841 gtgaaatttg cgcctgtggt gtggatgcta ttttagtcgg tggcagttta attatcggcg
     6901 gtggattcca cgaaaccctg gatattgtca aaactgaaag cgacgtgcca gtgataattt
     6961 tccccggttc ggtcgctcag gtttcgaacc gcgccgatgc ggttttatac atttctctca
     7021 tcagcgggcg caatccgaac gctctatttg gcgaacacgt aatcgccgca ccagttatcc
     7081 gcaaattagg cattgaaccg atttctaccg gttatatgct attcgaatcc ggcaagacta
     7141 ccacggctga atttatgagt aatacacgtc ctctgccgat caataagcct gaaatagcga
     7201 tggcgcatgc tttagccgct caatatttgg gaatgagtac agtttatctg gaagctggta
     7261 gcggagccga gcgctccatc cccaatcaac tcgtgaaagc cgtctgtgat tatatcgaca
     7321 taccggtatt cgtaggcggt ggtattcgta ctccggacga agcccgtgcc aaagtcgaag
     7381 ccggggcaac attcatcgta accggcaatg tctttgataa tgacgccaat atcggattgg
     7441 ctaaggagtt tgtccgagct gtgcatgtca aagagcattt ttagaccgaa gccatcttgc
     7501 accaaaaaat aatcgctcag ttagaagaaa gcgctgaagt aaagcgtcaa acaatcgtaa
     7561 cggcggttga ctcgatcgaa gccgccgccc aaatgttaat tgattgctat cgcgccggcg
     7621 gcaaggtgct tttatgtggt aatggcggca gtgccgcgga cgcgcaacac ctggcggctg
     7681 aattaatttc gcgattgaag ttagaacgag cggcgattcc agccttagcg ctgacgacca
     7741 atacttctct attgaccgct atcgggaatg attacaaata cgatttggtt ttcgtacggc
     7801 aagtggaggc ttttggccga gctggagatg tgctaatcgc catcagtacc agcggcgaat
     7861 ctgaaaatgt gatcaaagcc gtggaatttt cccgcgtgca gggtatcaaa tccatagcgc
     7921 tgaccggtgc aaaaggcggg cgtttagccg aaaaagttga tcttgcgatt aaaatccctt
     7981 ccgataacgt tcagcgtatt caggaatgtc atattaccgt cggtcatatt ctttgtgaat
     8041 taatcgagtc agctttatat ggaactatat gagccaattg atcttaaatg ataagcgaaa
     8101 ttcaagattt tatcaattat ctcaaggtcg aacgtaatct cgccccgaat actatccaag
     8161 cctacagcac tgatctggaa cgctatagtc aatttctaac ccaacaacaa attaatgaca
     8221 ttagcaaagt ccgttttagt gacatccaaa attacatcaa tgtattggct gaattgggat
     8281 tagcggccag aagtctggcg cgtaattaca gctcgattcg ggctttccat cgttttctat
     8341 ttagtgagcg catcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcttt
     8461 ttctaagtat gcgcggggct ccgctcaccc gaatggcggt ctggaatatt attcaggaat
     8521 atgtacgtct ggctgatatt cgcaaaccga taagccccca cgttttccgt cattcatttg
     8581 caactcattt attggaagga ggcgctaatt tgcgagcagt gcaggaaatg ctggggcacg
     8641 ccgacattgc aacgacgcag atttacaccc atctcgaccg cgaattttta atcgaacaac
     8701 accgcacttt tcacccgagg tattaatgaa ctttcaagta ataattctgc ttattccgcc
     8761 gattctcctg gctcttactt ttcatgagta tatgcatggc tggacggctt acaaaatggg
     8821 cgatccaacg gcaaaaatgg ctggacgtct gacaatgaac ccaattgcac atctcgatcc
     8881 gatcgggaca ttgatgcttt ttctggtaca tttcggctgg gcgcgtccgg tgccagttga
     8941 tccacgctat tttcgtaatc ccaaacgtga tatgctgata gtcgccgccg ccggaccgtt
     9001 agctaatatg tttatagctt tgctgagcgg tttattggca agactttttc agggcggata
     9061 ttttagtttc ctgccgctaa gtgtgctaca gccgctttat gtgatggtaa ttctcagctt
     9121 gcaaatcaac ctggcgctgg cgattttcaa cctactgcca attcctccgc tggatggttc
     9181 caaaatcctg atgggactgg ttccaccgcg ttatgaagcc cagtttaact ggttagagcg
     9241 atacggcagt tttattttaa tggctgttat aatagtcggg atggttacga atgtctctat
     9301 tctgggagct ttcattaatc cattcgtaaa ctttttctcg caattatttg gaggtatcta
     9361 ataatgtaca cgagacccac aaaaaaattt gacatatctg gcagacttga ggaacagcgt
     9421 gacgggaaat ttcattttaa ccgccaaagt agagttcctc ggaaatcacc gcgctctctg
     9481 ttatcaactc tgatatggat gatcattata ctattggtgt tgatgttttt aaaaaggcta
     9541 ttatagagtt cgttagaaag cacagttatc tttcatctcg tttagctacc agagtaatgt
     9601 atgaccgaaa taatagaatg cgttcccaat tttagcgaag gtcgtgatca cgatgtaatc
     9661 agggcaatcg cagtggcaat cgaaaaggtg aaggacgtaa aactactgga tgtcaattcc
     9721 ggtgctagtg tcaatcgcac cgtgataacc tttgtgggca atcgcgaaag cgtcattaat
     9781 ggggcttttg ctggaattgc cagagccgcc gagctgattg atatgcgtca tcatcgcggc
     9841 actcatccgc gactcggagc gaccgacgtc tgcccgcttg tccctgtttc cggaataagc
     9901 gtggaagaat gcattgctct ttcgcaccaa ttagctaaac gtgtcgctga agaactacac
     9961 attccggttt atctttacgc aaaatctgct caaaaaccag aacgcattga tctggcaaat
    10021 atccgcgccg gagaatacga gggtctcaaa caaaaactca gcgatccgaa ctggcaaccc
    10081 gattacggac aaccgatttt caatcccaag tccggggcga cgattatcgg cgttcgtaat
    10141 ttcctgatcg ccttcaatgt taatctcgat actcgcgact gtcaattagc aaaggacatt
    10201 gctctaacga ttcgcgagaa aggtcgaatc aaacgcgatc aaaatggaaa taaaatgtat
    10261 aattcccaag gtgaaccaat ttacgaaacc ggtttattga aaaatgtcaa ggcggttggc
    10321 tggtacattg acgaatataa aatggcgcag gtttcgacta acttgacgaa ctacaaagtt
    10381 accccgccgc acgtcgtttt cgaagaaatc cgacggctcg ccaatgccag aggtctgcgc
    10441 gttacgggtt cggagctggt cggactgatt ccaaaggagg cgcttctgat ggctgggcgt
    10501 tattatctga aaaaacaagg ctattcggaa gacgctccgg aaagcgagct catccagatg
    10561 gcagtgcaat cgctcggttt gaatgccatc gcgcctttcg atcccgccaa aaagattatc
    10621 gaatatcgtt tggcggagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    10741 tccagcacag aaaaattaaa agaattggtt aaattactga aacaataatg aatggatttc
    10801 agaattaatg tcgaaaataa aaatattatc ggaaaatctg acaaataaaa ttgccgctgg
    10861 agaagtcgtc cagcgtccgg cttcgattgt caaagaatta ttcgaaaaca gcatagacgc
    10921 cggcgccgac gaaattatcg tcgttattcg caacggcggt aaaacattat gcgaggtggt
    10981 tgataacggt gagggtatga atcgcgatga tcttctcctg gctttcgaac gatatgctac
    11041 cagcaaaatt gccaccgtcg aagacctgac caatatccac accctcggtt tccgcggcga
    11101 ggctctggca agcattgcgg cggtagcaat cgttaacgcc atctctactc tgcggggcgc
    11161 aaatgaaggt tatgaattac gcatcgaagg cggcacgttc cgcgacatca gacctaccag
    11221 tcccaaaccg ggcacgacaa tttccgtccg taatttgttt tacaatattc ccgcccgtcg
    11281 caaatttctg aaaagtgccg aggttgagtt tcgtcatatc gtcgatgtta tgcaagaatt
    11341 tgctctcatc catcacgatc tgcgttttat ttttatccac aatgatagag aaatttacaa
    11401 cttgtcgcca agcagtctgg cagaacgcat cgctaatctc tttgccccag aatatagtca
    11461 aaatcttcta ccggttgagt cggtcgcaga tgatctaaaa gtcaccggct atatcgggaa
    11521 cctgaatctg gtgcgcgcgc ggcgtggtga acaattttta tacatcaatc gtcgctttgt
    11581 gaatgatcgt ttgatgaatc atgcgataat cagcggctat ggcggattgc tggcgcggag
    11641 cgaattccca ttctattgcc tgaacctaga actgccgccg gaacaaattg acgtcaatgt
    11701 ccacccgact aaaatggaag taaaatttcg tgagcaagat cgcgtttatc agtttttaaa
    11761 gcaagccact gcgaacagcc tgaggtcaat tacaaacgtc attcctgatt taggaacttt
    11821 tgcgccgcac cattattata ctacacctcc gccgccgaaa ggaacgcaaa ttcagatccc
    11881 ggctccggat caatctcctt ctaaacagtc agaatttgat tcatcgagaa aaactttcga
    11941 tgttccagaa gtagaaatcc cgccgacacc gttcgccgcc ggcgacagca ccaagtggca
    12001 ggaacgggct cggcgttttg tcgagcaggg cgagaccagc gaggctgatg attttcgtcc
    12061 cagtgtagcc gtttatcaag tccataataa gtatatcatc agtcaggtca agtccgggct
    12121 ggtgattatt gatcagcatg ttgcccacga acggatcctc ttcgaagagg ctcaggaatc
    12181 tatgcgcggt gaaaattgga aagcacaaca gttgttgttt ccggtagttg tcgagctcac
    12241 cgtgaatgat ttttccactc ttctggagat attaccgttt ctggaaaaac tcggattccg
    12301 tattcgggaa tttggcaaac agacggttgc cgttgaagcg gttccggccg gcttgcgctg
    12361 gggcaatgaa aatatcatca tccgcgaaat tctcgatcac taccaggaat tcggcaccaa
    12421 agacaccagc attcaggata aagtcgccgc cagttatgct tgtaaagccg ctatcaagtc
    12481 cggcgatccg ttgactacgg aggaaatgca aaacctggtg gatcgcttgt ttgcgaccag
    12541 caatccttat ttttgtccac acgggcgacc aattatcgtc aatctgtcgc tgaaagaact
    12601 ggataaacgc ttcgaaaggg cttagcaatt gagtgctaat tccaactcga tcaatgaagc
    12661 gctttctcga aaaatccctt ttattgttgg tcctacggcg gtagggaaaa ctgcgactgc
    12721 gattgaactg gcaaagcagt tgaatggtga aattatctca gttgattcgc ggcaagtata
    12781 tagagggcta gatgttggca cggctaagcc gactttgcga caacagcaag aaattcccca
    12841 ccatttagtt gatattctcg atctcgatga gcagattagc gctggcgctt accgcaattt
    12901 agctttaaaa acggtcagtg aaatccagtc ccgcgggaga ttgcctattt tcgtcggcgg
    12961 ttccggattg tatgttaatg ccgtgctgaa aggaattttc caagaaagca ctactaatcc
    13021 tgagatacgt cgggaaatcc ggcaagaatt acaggaaaaa ggcgttgtgg cgctttataa
    13081 tcagctcctc gaaattgacc cggatacggt tttgaaaatt catataaacg atgtcaagcg
    13141 gataacccgc gcccttgaaa tttaccgcat caccggccag ccacccagnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aactcagatt ctgccgataa tccaatgaaa
    13321 ctatggtatc aacagccagc caaacaatgg gtcgaagcgc ttccaattgg taatggtcgt
    13381 ctgggtgcca tgatttttgg tgatccacgg cttgaagaaa ttcagctcaa tgaaagcact
    13441 atctggggtg gtcagcctca tcgcaatgat aatcttgaag ccaaagaagc attgcccaaa
    13501 gtccggcaat tgatttttaa tggtaaatat aaagaagccc aggaactagc ggatcaaaaa
    13561 ttcattagta aaaattcgca gggtatgccc taccaaaccg caggaagtct ttacctttct
    13621 tttccggaac acaaaaactt tacaaattat tatcgagagc tgaatattga aactgctgtt
    13681 gttactaccc gctatgaagt tgatggagta acgtataagc gtgaagtttt tgcctcattt
    13741 cctgatcagg ttatcatttg ccgaatctca acgagcaaac ccggtaaaat caattttact
    13801 gcttcaatga atcatcctgg cacagtaaat attttcactg agggaaatga tatattgatt
    13861 atgtctggaa taacgagcga ttgcgattcg ataaagggcg aagtaaaatt tcaggtacag
    13921 gtaaaagtaa ctacagaaag cggctcaatt gaggcatctc aaaacagcct aactgtaaac
    13981 aatgcagata ttgctacaat ctatatttca atggcatcga gttttaaaaa ttacagcgat
    14041 attagcgaaa atgctggcga gaaagcacag tcgtatcttc gaaacgcctt gagaaaaaat
    14101 tacaaacaag cgttagaaaa tcatattgct gattatcagc gctattttaa gcgagtcagt
    14161 attaatttag gagtaactga ttctataaaa aatccgaccg atgttcggat tgaacgattt
    14221 gctaaaggta acgatccgca attggttgca ttgtgttttc agtttggtcg ttacttgctt
    14281 atttcttcat cgcgccctgg tacacagcct gctaatctgc agggaatttg gaacaaccag
    14341 ctttacccac cctgggatag taaatataca gtgaatataa acacggagat gaactattgg
    14401 ctttctgagc caaccaattt aagcgaaatg aatgagccgc tagttcagat gattgaagaa
    14461 ctctctgaaa caggcagaca aactgcggaa agtatgtatg gtgcggaagg gtgggtattg
    14521 catcataata cagatatttg gcgtatttgc ggaccggtag atggtgcatt ttggggaatg
    14581 tggccaatgg gcagtgcttg gctttgtcaa catttatttg aaaaatatga atatagcggc
    14641 gatagagaat atcttaaatc tgtgtatcca gcaatgagag gcgctgttga attttatctt
    14701 gactttttag ttgaagaacc ccaatacaaa tggttggtgg tctgtccatc ggtttctccg
    14761 gaaaatgcgc cttcctgtca tccggaattt tcaatttcag ctgggacaac tatggacaat
    14821 cagctccttt ttgacctatt tactaaaaca attaaggtgg cggaaattct caacactgac
    14881 aaagaatttg tcataaaaat aaaagaaacc ttgcatcaat tgccgccaat gcaaattggc
    14941 aggtgggggc agttgcaaga atggatgcag gattgggatg atcctgaaga ccaccatcgc
    15001 catgtttcgc atttgtatgg cttatatcca tccaatcaaa tatcgcccta tcgaacaccg
    15061 gaattatttt cagccgccaa aacttcttta ttagctcgcg gcgatgaatc caccggttgg
    15121 tcaatggggt ggaaagtgaa cttatgggct cgcctccttg atggcgatca tgctttgaaa
    15181 ctgattacca atcaattatc tccatcaatc caaccagatg gaagccaaaa aggcgggaca
    15241 tatccaaatc tgttcgacgc tcatccacct tttcaaattg atggaaattt tggatttacg
    15301 gcaggcgttg tggaaatgat tgtacaaagc tatgatggct ttatctttcc attgccggca
    15361 ttacctgaaa tttggaaaag cggaagtatt accggtattc gtttacgcgg aggatttgaa
    15421 attgtgagat tagaatggca aaatggtaaa atttccacgt taactataaa atcaaactta
    15481 ggcggtaatt gtagaataag aagctacgat aagcttattc ctatgggcaa ttttaaactt
    15541 gaagaagcaa aaggggaaaa tcccaaccca ttttatgcta tatctcaagt aaaggaacct
    15601 ttgatttctc ctgatgcgaa tattattcct gatgaacttc gcaaaagtca tctgtatgat
    15661 tttaatacta aagctggaaa ggaatacact ttgatcttat tagagtaatg attaaaattt
    15721 atgaaaagaa tcttgcccga aaagtttacc atccgattag cgtttagaga attaaaacta
    15781 gtattaatga tagatttaat aaatgtatat ctctggtagt atttatatta gtccaatttt
    15841 aatgtaaaag tgagaggaaa atgagtaata acggtattct tttaaggatt ctccatattt
    15901 gtagcgtttt gatcatattg attcaatcgt cggttatttc tcaagtcata cttgatccag
    15961 atgccgtagt tgaagagata gtttctggaa ttcagcaacc ggaaggaccg gtttggagag
    16021 aaggcctcgg tttattattt tctgatatta agggaaacaa gatttataaa tggacttccg
    16081 aaaaaggcaa ggagatttat ttagatcctt cctataacac taatgggtta gcttatgatt
    16141 tggaaggtcg ccttgttgcc gggcagatgg ggcttagaag agttgttcgt tttgaaaaag
    16201 atggctcgca aacatcgctg gcagatagtt atgatggaaa gaaattaaac agtccaaatg
    16261 atttggttat taaatctgat ggtgcgattt tcttcaccga tccagatttt aatataccat
    16321 caggagaaag caaggaactc aacttcaatg gaatttatag aatcagccct tcaggtgttc
    16381 ttcagcttct gacgtcgtca ttgactttgc ctaatggtat ctgcttttct cctgatgagt
    16441 ccaaactcta tgtcaatgat tctcaagtac ataaaattta tgtatgggat gtggttgatg
    16501 attctaccat aaccaataaa aaactattat atactattcc cgtaccattc ggctatgcgg
    16561 acgggatgaa aattgatgaa gatggtaata tttactgtac ctgctcgtct tatgtttggg
    16621 ttatttctcc tgagggcgaa ttaattggga aaattgattt accaacgagt gtttctgctt
    16681 caaattgtgc ctggggcgat gatgacaata ggacattatt cattaccgct ggtaactcgg
    16741 tttatagagt gcgtccgttt gttacttcaa tcaataaaga agactcgagt ttacctaaga
    16801 gttttggact ttctcagaat tatcctaatc ccttcaatcc gactacaaaa attacttatt
    16861 caatcccagt tacttcaaat gtaatgataa cggtttatga tgtgtcggga aaagaagtag
    16921 tgacgataga ggattgtcag aaaaaagccg ggacttatac tctattgttc aatgctgaga
    16981 aattagcgag tggagtatat tactacaatc ttaccgcgaa tgaattttca tccaccaaaa
    17041 aatttgtgat attaaaatag ttttaggtgg tcatttgaga ttattaatta aaaaatataa
    17101 ccaattaata ggagcgtaag catggaaaaa ttaatcagaa agtcactatg tacagcatta
    17161 ttattgtgtt taatgagcag tactattttg tactccca
//
LOCUS       DAOMJU010000040        16922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_3270_length_16922_cov_22.770380, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000040 DAOMJU010000000
VERSION     DAOMJU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 16922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16922
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3270_length_16922_cov_22.770380"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..823
                     /locus_tag="PLO58_05375"
     CDS             224..823
                     /locus_tag="PLO58_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74255.1"
                     /translation="MDVKLDSLIEKIKQEGIEEAQRAADEIRQKAEAEAQEIINKANE
                     KARQIINEGQQNAEKLRANAEAAIRQAARDTILVTKENLLKLFDRVFKKEIAKTLTPE
                     FLKEMILALVKSTSSDNQYEFVVAEKDIEKLKTLVLAATRDSLKEPVTFRVDRGIAAG
                     FRVSRKGEEVYYDLTDQSIAEVLQEFLNPALRQLLDQNG"
     gene            816..1313
                     /locus_tag="PLO58_05380"
     CDS             816..1313
                     /locus_tag="PLO58_05380"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HQO74256.1"
                     /translation="MDKYYYLIAQLPVLYFEREPALTIEQFLTEAYKWLSAEDYAQLE
                     KVDLNATAPDKNDPDIIRRIKNFELDLRLELVEWRTAQKKNQDYKIIAFPANLVREGD
                     PLTVEKNLLLWRWQFLDSLEFGHYFDLEFLIMYYLRLQILKRLFTFNKKIGREKYQQY
                     LEMEL"
     gene            1310..3049
                     /locus_tag="PLO58_05385"
     CDS             1310..3049
                     /locus_tag="PLO58_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HQO74257.1"
                     /translation="MKKYGKITSINGNMITVACESDIIQNEVGYAVVGDENLKAEVIR
                     IQGNLAYMQVFESTKGLRVGDKVEFSGQMLSVRLGPGLLGMVYDGLQNPLHDLAAKFG
                     FFLKRGAVIEPLDSKKEWDFKPLVKSGDIVQAGISLGNVSEGIFEHQIFVPFNLRGEY
                     AVKTIVPEGKYKITETIAVITNEKGDEINLKMNFEWPVKIPITAYREKFVPTEPLVTQ
                     TRIIDTFFPISKGGTYCIPGPFGAGKTVLQQVTSRFAEADVVIIAACGERAGEVVETL
                     REFPELTDPRTGRSLMDRTIIICNTSSMPVAARDASVYTAATIGEYYRQMGLNVLLLA
                     DSTSRWAQAMRELSGRLEEIPGEEAFPAYLESRIAQFYERAGIVELLDGSRGSLTIGG
                     TVSPAGGNFEEPVTQSTLKVVGAFHGLSRDRSNARRYPAIHPLESWSKYTSFIPSNLI
                     EDARQILFKGNEVHQMMLVVGEEGTSLDDFEIYMKSEFLDAVYLQQNSFDQVDGASSP
                     ERQKYVFSKVHEVLMTKFNLTDKNDARDFFNKLRQVFIDWNYIDFKDDEFKIQEQKIN
                     QLIKEKARDAKSL"
     gene            3033..4343
                     /locus_tag="PLO58_05390"
     CDS             3033..4343
                     /locus_tag="PLO58_05390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HQO74258.1"
                     /translation="MQKVYNKILSVTGNVITVKASNVANEELAEISTQRGKSLAQVIK
                     LDGDIVSLQVFAGSQGIATNDEIRFLGHPMQVTFSDNMLGRIFNGSGVPRDKGPQLTE
                     NMIQIAGPSVNPAKRIIPRRMVRTNIPMIDVFNSLVISQKLPIFSVSGEPYNELLARI
                     ALQAEVDMIILGGIGLKYDQYMFFKNTIEEGGALSRSIFFIHTASDPIVEGLMVPDLS
                     LAVAERFALKGRDVLVLLTDMTNFADALKEIAITMEQVPSNRGYPGDLYSQLAARYEK
                     AVDFEGAGSVTILAVTTMPGDDVTHPVPDNTGYITEGQFYLRNGRIEPFGSLSRLKQQ
                     VNKSTREDHRAIMDGMIQLYAQFKETEEKRSMGFRMSDWDRKLLKYGERFENDLMDLK
                     VNIPLESALNLGWQILADCFEPQETGMKSELVQKFWPKKAEVSA"
     gene            4359..4970
                     /locus_tag="PLO58_05395"
     CDS             4359..4970
                     /locus_tag="PLO58_05395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HQO74259.1"
                     /translation="MAKIKHTKNELKAQREALARYERFLPTLELKKSQLVTEIRHLSL
                     AIEQVEEEIRKITEQVFTWVDVMAEEVDLKAWLKIKEIHTDEGNIAGIEIRIFRQVEF
                     EDVPYDFFTTPLWVDRALSILKEQIARRIKIKILQEQIEVLQQELRVTIQRINLFEKV
                     KIPETRENIRIIQIFLGDAQTAEVVRGKIAKSKIQSKREALKV"
     gene            4979..6817
                     /locus_tag="PLO58_05400"
     CDS             4979..6817
                     /locus_tag="PLO58_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HQO74260.1"
                     /translation="MKKVVLFVHTPERDSALKKLRKLGVVHIQHIKPPISEDTSKIEN
                     AIYAVEKSLGIIEGYNTPPSSENVDLEKCQKLVSDIIALETEKQNLKSKIDEKNIYLN
                     WYNRWGIISEKDVAELKEHRIYLHLYVIDRSILKNIPEEAAVEILKEEKNQLLIAFIA
                     RSEDVRLDLKEEQIPPEDFDTVTRELQNFTSRLAEIEQQLTELAKKAGLIRAYQQELE
                     KHLEFARVRSGMQDAEEITYLQGYCPSDDVSQLMQAASVEGWGILSDDPDETDNPPTL
                     LKMNKIARLIQPVLDFLGTVPGYREYDISQYFLIFFALFVAMIIGDAGYGLIFFLVAI
                     FAHYKLVKQGKSLPLFLKLLYLLSICTIGWGTITGNWFGSIQIAGLPLLKALTIPQLA
                     TFPELFPDLTVNPQDQIMFICFTVGVTQLGLANIMNFINNLPHLKALGNLGWFGVTVG
                     AFLLVLNLVLGSPLPSFTVPLIGCSVVAIVLFMRQESGTSFLKGIARGLGDGFATFLN
                     VISSFSNIISYIRLFAVGMATVAIATSFNQIAASLLKGFTLPAGMLILIIGHGLNIVM
                     SILSVIVHGIRLNVLEFSSQLGMEWSGYKYEPFREKIKNLTEREIQ"
     gene            6814..7236
                     /locus_tag="PLO58_05405"
     CDS             6814..7236
                     /locus_tag="PLO58_05405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455682.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HQO74261.1"
                     /translation="MNIGLIGPAAALGLAALGSVLGCGIAAQTAVGGWKKCFANNKPA
                     PFMLVAFAGAPLTQTIYGFLLMLFINGSDQEPLVKLCAGVFGGLAIGFSAFFQGKVAA
                     RAADALAETGKGTANYFMVIGIVETIAVFVLVFMLLNL"
     assembly_gap    7363..7462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7748..9139
                     /locus_tag="PLO58_05410"
     CDS             7748..9139
                     /locus_tag="PLO58_05410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQO74262.1"
                     /translation="MPSLTFLGATGTVTGSRFLLKTAHKQYLIDCGLFQGLKELRLRN
                     WEPFPISPEDIDAVILTHAHIDHTGYLPRLVHYGFKGTIYATTATTDLCQILLPDSAH
                     LQEEDAEYANKKHFSKHSPALPLYTTEDALKALELFKPIAYGSRLQLDDGVSLTFQDA
                     GHILGSSFIDLRLPIDGEERRLLFSGDLGRPNQPILRDPHTVFGADYLIVESTYGDRL
                     HGNEDPKEVMARIINESIRRGGVLLIPSFAVGRTQELLYTIRELEEANRIPHLPIYMD
                     SPLAIKATKIFSKNKQDFDLKSKTLELKGKNVLETAQLHFAQTQEESKAINKIKSNAI
                     IISASGMATGGRILHHLFNRLPDPKNTVLFIGYQAVGTRGRTILDGAESVKIHGQYVP
                     IRAHIESTSCFSAHADYHEILAWLSNFNSVPRKVFIVHGEPEPARSLSDKINRHFGWS
                     TELPEYLSQYQIS"
     gene            9172..10506
                     /locus_tag="PLO58_05415"
     CDS             9172..10506
                     /locus_tag="PLO58_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQO74263.1"
                     /translation="MDLFEANPQNTNLLPPLAERMRPRRLEDFKGQEEIVAQGKILRK
                     ALESRKIFSMILWGPPGSGKTTLAKIIANYVDADFYQISAVSAGVKDVRAIIDKAAYN
                     RSRNRSTLLFIDEIHRFNKAQQDALLHAIEDGSLILIGATTENPSFEVISPLISRCRV
                     LKLEGLSDENLASIINTAIAKDIILLKYKIKFEPGAQDFLIKNVNGDARKLLNAIEIC
                     QQIAAPEEGAEFTITIDLIKEALQQRRQLYDKTGDYHYDTISAFIKSVRGSDPDAAVY
                     WMALMLEGGEDPLFIARRLIILASEDIGNADPSALPLATAGFQAVHAIGMPEAAIVLA
                     QVTTYLASTFKSNASYMALENARQALKEGSAPSVPLHLRNAPTKLMKAQGYHKGYRYP
                     HSYPDHFVKENYFPGDGLAPTFYQPTSIGREKFLRERLEQLWPERYRRTKNS"
     gene            10767..11024
                     /locus_tag="PLO58_05420"
     CDS             10767..11024
                     /locus_tag="PLO58_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQO74264.1"
                     /translation="MRRKIVRVGASLCVLLPKEVVRAMGWDFEDEINLILDEENEVVI
                     LRNLQNQPIEKKTMMEHFDKFLENYGKELDTLVADPEPQIQ"
     gene            11039..11281
                     /locus_tag="PLO58_05425"
     CDS             11039..11281
                     /locus_tag="PLO58_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQO74265.1"
                     /translation="MKRKIIRVGSSKGVLLPREVTKAMDWGFGSEIELSVDEEKHEVY
                     LKSLKVSVPQDYEIDHLRQLEEVLEDYADLLEGIDD"
     gene            11274..11672
                     /locus_tag="PLO58_05430"
     CDS             11274..11672
                     /locus_tag="PLO58_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74266.1"
                     /translation="MTEEQIRQIKLISYANWLVTEKFGAEKGIRDIKALTAAVTLPGN
                     GTLSCVLGERLSNRKDLYAQLGWFVYYMIEGEPFNSKNRTTVLLILLWVLKYYGLEFN
                     AEELANYIKTLDPLKHGNSDISLWFSTHCR"
     gene            12020..12949
                     /locus_tag="PLO58_05435"
     CDS             12020..12949
                     /locus_tag="PLO58_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74267.1"
                     /translation="MRQVVDFGFAWNWEYDWDFANLLEANCQSEGLNFLQVTPEKIKS
                     VLTELGQGGIYLQALWDRAADTDSRFIELINWAKNNNILQINPHEKAVRAWNKATMHL
                     EFITAGVNTPYTIVLDPYNVRPILPEIDLSVLGGRFIIKPAHGGGGLGVILDATSLER
                     VLTARRENPDDFYLLQAVIEPLQVNGRPAWFRVIYGFGKCYFCWWDVKTHIYTPFSNN
                     DKESLPMPLLNDIIEKIADVTGLDVFSSEIALTAEGKFVVVDYINDPVDLRLQSRAAD
                     GVPDEIVRAISSDCVATVKRAVQAKATYEKLEN"
     gene            13177..13725
                     /gene="pyrR"
                     /locus_tag="PLO58_05440"
     CDS             13177..13725
                     /gene="pyrR"
                     /locus_tag="PLO58_05440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQO74268.1"
                     /translation="MEFRVKARLADEQGVNRTITRLAHEIIENCKGTENVGIIGIRTR
                     GDYLAKRLAHKIEEIENVKLPVGVLDVVMYRDDFRMKTKLPRVEVTDIPFDVDGKILI
                     LVDDVIYTGRTIRAALDALMDFGRPAAIQLAVLVDRGHRELPIKPDYVGKNIPTSIGE
                     EVRVMIKEIDGRDEILLVEEQK"
     gene            13722..14642
                     /locus_tag="PLO58_05445"
     CDS             13722..14642
                     /locus_tag="PLO58_05445"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQO74269.1"
                     /translation="MNLSIKHLLGLEGVPRDDISTILDTAFSFREVLERPIKRVPTLQ
                     GKTIVNLFFENSTRTRISFELAEKRLSADTVNFSSSGSSLAKGESLKDTVRNLYAMKT
                     DAVVMRHGVPGSAKLLTQFIDAVVINAGDGTHEHPTQALLDMMSLQEKFGSLDGLNIA
                     IIGDIRHSRVALSNIYGLLTMGANVALCGPPTFMPVGIESLGVKVIYDLDEALAFADA
                     INILRIQRERQGKGMIPSLREYRAVFGITRERLDKLHKTITILHPGPINRGVEIDSDV
                     ADSEHSLILHQVLNGVAVRMAVLFLLLGGK"
     gene            14665..15957
                     /locus_tag="PLO58_05450"
     CDS             14665..15957
                     /locus_tag="PLO58_05450"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQO74270.1"
                     /translation="MQIKAINEELLLQNAVIVDPFKDNIYEADILIGKGKIKNIEQKI
                     DPKNVKICEDLKGSYVAPGFIDLHTHLREPGNEDEETIATGVRAALAGGFTKICCMPN
                     TDPPIDTQEAVRFIYRQAENLLAEVYPIAAITKGRQGAELTEMVELANAGAVAFSDDG
                     APLKNAQIMRYALEYSRLTGKPIINHAEDIDLKADGLMNEGVVSTHLGLPGNPAIAEE
                     IMINRDLQLAKFTSARLHVPHVSTAGSVELIRQAKAEGVHVTAEVTPHHFSLTDEYMR
                     TFDANGKVAPPLRTEADRQALIAGLKDGTIDAIATDHAPHTIDSKETSLDLASYGMIG
                     LETAFALAMTNLVHPGHLTLLQLIKLLTVNPAAIMNIALPKLENGVDANLTIFDPNQW
                     WVFNRKNIYSRSQNTPFIGAELTGRIKAVVTKSHYISL"
     gene            16032..16727
                     /locus_tag="PLO58_05455"
     CDS             16032..16727
                     /locus_tag="PLO58_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQO74271.1"
                     /translation="MTSDIKSHKKQSALSTSQTVIAFQKKVRKHFHGNYLLHLPPGYA
                     ESQNDWPTIFFLHGSGERGNDPEIVRRQGLPQVIDKKPDFPFIVISPQCPANRWWSLE
                     WLDALFDEVVKLYRIDQKRIYLTGLSMGGFATWDWAIEHPERFAAIAPICGGGNPLEA
                     DKIKDVPTWVFHGAKDEIVPLQKSQEMVAALKAGGGKVKFTVYPNTGHDAWTKTYNNL
                     RLYEWFLKQTKND"
     gene            16720..>16922
                     /locus_tag="PLO58_05460"
     CDS             16720..>16922
                     /locus_tag="PLO58_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HQO74272.1"
                     /translation="MTDWIAIKKDEKHIAREKAKARELRQSNWWKQKIAAGICYYCGK
                     KFKPQELTMDHIVPIARGGKSIK"
BASE COUNT         4865 a         3451 c         3922 g         4584 t
ORIGIN      
        1 ttattatttt taatcatcca aaaagcagga attatatttc ttgcttacga agtaagggtt
       61 ttttgaaatc gtttgggatt aatcctattt atattcaaat ggtaaatatt ctgtcttgat
      121 gttatccaaa aaatcttcaa ccttgcttga cttaacaatt catagaagtt aaattcacaa
      181 gttattttct aatgaataag aagccgaact acaggagatt tttatggacg ttaaactgga
      241 cagtttaata gagaagataa agcaggaagg catcgaagaa gctcagcgcg ccgccgatga
      301 aatccgccag aaagccgaag ccgaagctca ggaaattatc aataaggcta atgaaaaagc
      361 ccgccagatt atcaatgaag gtcaacagaa tgccgaaaag ttgcgcgcta acgcggaagc
      421 cgcaattcgc caagccgccc gtgatacaat cctcgtaact aaagaaaatc tcctgaaact
      481 tttcgatcgg gtttttaaga aagaaatagc caaaacattg acgccggaat ttttaaaaga
      541 gatgattctg gcattggtaa aatctacatc ttccgacaac cagtatgaat tcgtggtagc
      601 cgaaaaagat attgagaaat tgaagacttt ggtattggcg gcgactcgcg atagtctcaa
      661 ggagccggtt actttccgag ttgaccgtgg tattgccgcc ggctttcggg tttcgcgcaa
      721 aggtgaagag gtctattatg atctaaccga ccaaagcatc gctgaagttt tacaggaatt
      781 tctcaatccg gctctgcgcc aattattaga tcagaatgga taaatattac tatttaatcg
      841 ctcagttgcc tgttttatat tttgagcgag aaccggcgct gaccatcgaa caatttttaa
      901 ccgaagccta caaatggctt tcggctgagg attatgcgca attggaaaag gtggatttga
      961 atgccacagc gccggataaa aacgatcccg atattattcg tcgaatcaaa aattttgaat
     1021 tagatttacg cctcgaactg gttgaatggc ggacagccca gaagaagaat caggattata
     1081 aaattatagc gtttcctgct aatttagtgc gagagggcga tcccttaacc gttgaaaaga
     1141 atctcttact atggcgctgg caatttttag attcgctgga attcggccat tatttcgatc
     1201 tggaatttct cattatgtat tatctacgac tccagatttt aaaaagacta tttactttca
     1261 ataaaaaaat cggacgtgaa aaatatcaac aatatttaga aatggaatta tgaaaaaata
     1321 cggaaaaatt actagcatta acggcaatat gatcaccgtc gcttgtgaga gcgacattat
     1381 ccagaatgaa gtcggttacg ctgtggttgg cgatgagaat ctcaaagcag aggtgatccg
     1441 gattcaaggc aacctggctt atatgcaggt cttcgaaagc accaaaggtc tgagagttgg
     1501 cgataaggtc gaattcagcg gacaaatgct ttcggtgcgg ctcggtcccg gcttgctcgg
     1561 tatggtttat gacgggctcc agaatccttt gcacgacttg gcggctaaat tcggcttttt
     1621 cctaaagcgc ggagctgtta tcgaaccgct ggattcaaag aaggaatggg attttaaacc
     1681 gctggtcaag tccggcgata ttgttcaggc tggtatcagt ctcggcaatg tctcggaagg
     1741 tatttttgag catcaaattt tcgtgccttt caacctgcgt ggcgaatatg cggtcaaaac
     1801 gattgtgccg gagggtaagt ataaaataac cgagacaatt gccgtaatta ccaatgaaaa
     1861 aggtgacgaa atcaatttga aaatgaactt cgaatggcca gtcaagattc caataacagc
     1921 ctatcgtgag aaatttgtac cgactgaacc gctggtgacg cagaccagaa tcattgatac
     1981 tttcttcccg atttccaaag gcggtaccta ttgtattccg ggacctttcg gcgccggtaa
     2041 gacggtactt caacaggtga ccagtcgctt tgccgaagcc gatgtcgtaa tcatcgcggc
     2101 ttgcggtgaa cgtgccggtg aagtagttga aactctgcgc gaatttcctg aactgaccga
     2161 tccgcgcacc ggtcgctccc taatggatcg caccataatt atttgcaata ccagttcaat
     2221 gccggttgcc gcgcgcgacg cctcggttta tacggccgca acgattggtg aatattatcg
     2281 ccagatggga ttgaatgttt tactactggc ggattccact tcacgttggg cgcaggcaat
     2341 gcgggaacta tccggtcgtc tggaagaaat tccgggagaa gaagccttcc cggcttatct
     2401 tgaatcgcgc atcgcccagt tttacgaacg cgccggaatt gtcgagttgc tcgatggtag
     2461 tcgtggtagt ttaaccatcg gaggtaccgt cagcccggca ggcggtaatt tcgaagagcc
     2521 ggtaactcaa tcaaccttga aagttgtcgg cgcttttcac gggctttccc gcgatcgctc
     2581 caatgcccgc cgttatccgg ctattcatcc cctggaaagc tggtcgaaat acaccagttt
     2641 cattccctcg aatttaatcg aggatgcgcg ccaaatactt ttcaagggta atgaagtgca
     2701 ccagatgatg ctggtagtcg gtgaggaagg cacttcctta gatgattttg aaatttatat
     2761 gaaatctgag tttctggatg cagtttactt acagcagaat tctttcgatc aggtagatgg
     2821 cgccagtagc cccgagcgcc agaaatatgt cttcagcaag gtgcatgagg tattgatgac
     2881 caaatttaat ctgactgata aaaacgacgc ccgcgatttt ttcaataaat tgcggcaagt
     2941 cttcattgac tggaattata ttgattttaa ggacgatgaa ttcaaaatac aagaacagaa
     3001 aataaatcaa ttgatcaagg aaaaagcccg cgatgcaaaa agtctataat aaaattttaa
     3061 gcgtaaccgg caacgtaata acagtaaaag ccagcaacgt agctaacgaa gagctggcgg
     3121 aaatttctac ccagcgcggt aaatctctgg cgcaagtcat taaactggat ggcgatattg
     3181 tttcgctcca ggtattcgct ggcagtcagg gcatcgccac caacgatgaa atccgtttcc
     3241 tggggcatcc gatgcaggtt actttctcgg ataatatgct cggtcgcatt ttcaatggca
     3301 gtggtgtgcc gcgcgacaaa ggtccacaat taacagaaaa tatgatccag attgctggtc
     3361 cttcggtcaa cccggctaag cgcatcatac ccaggcgaat ggtcagaacc aatatcccaa
     3421 tgattgatgt gttcaattct ctggttattt cgcaaaaatt gccgattttc tcggtttccg
     3481 gtgaaccata caacgagctt ttggcgcgca ttgcattaca ggcggaagtt gatatgatta
     3541 tactgggcgg catcggtctg aaatatgatc agtatatgtt ttttaaaaat acaatcgaag
     3601 aaggtggtgc cttaagccgc tcgatatttt tcattcacac ggcttccgat ccaatcgtag
     3661 agggtttaat ggtgccggat ttgagtttag ccgtggcgga acgctttgca ctcaaaggac
     3721 gtgatgtgct ggttttgctg accgatatga ctaattttgc cgatgcgctc aaggaaattg
     3781 ccattacgat ggaacaggtt ccatccaatc gcggttatcc cggcgatctc tatagtcagt
     3841 tagccgcgcg ttacgaaaaa gcagttgatt tcgaaggcgc cggttcggtg accattctgg
     3901 cggttacgac tatgccgggc gacgatgtta cccatcccgt tccggacaac accggttata
     3961 ttactgaagg acaattttac ttgcgcaacg gtcgcatcga gccttttggt tcactcagtc
     4021 gtctaaaaca acaagtcaat aaaagtacgc gggaagatca tcgcgccatt atggacggta
     4081 tgatccagct ttacgctcag ttcaaggaaa ctgaggagaa gcgctcgatg ggattccgga
     4141 tgagtgattg ggatcgcaag ttgctgaaat acggcgagcg gtttgagaac gatttgatgg
     4201 atttgaaggt taatattccg ctcgaatcag ctctaaacct cggctggcaa attctggcag
     4261 attgtttcga acctcaggag acaggaatga aatcagaatt ggtgcagaaa ttctggccaa
     4321 agaaagcaga agtgagcgct tgattcatat aaataatcat ggctaagatt aaacatacca
     4381 aaaatgaatt aaaagctcag cgcgaagcct tagcacgcta cgagcgcttt ctcccgactc
     4441 tcgagttaaa gaaaagccaa ctggttactg aaatccggca tcttagtctc gctatcgagc
     4501 aggttgagga agaaatccgg aaaattactg aacaggtgtt tacctgggtt gacgttatgg
     4561 ctgaagaggt agatttaaaa gcctggttga aaatcaaaga aatccacact gacgaaggca
     4621 atattgccgg aatcgaaatc cggattttcc gccaggttga atttgaagat gtgccctatg
     4681 atttttttac aacgcctctg tgggttgatc gcgctctcag catccttaaa gagcaaattg
     4741 cacgccggat taaaattaaa attttacagg aacagatcga agttctacaa caagaattac
     4801 gcgtcaccat tcaacgcatt aacttgttcg agaaagttaa aattcccgaa actcgggaaa
     4861 acatccgtat aatccagatc tttctcggcg atgcgcagac ggcggaagta gtgcgcggta
     4921 agattgctaa atctaagatt cagagtaaaa gagaggcgtt aaaggtatga tagttccgat
     4981 gaaaaaagta gtgctttttg tgcatactcc ggaacgagat tccgctctga agaaattacg
     5041 caaattagga gtggttcaca tccagcatat caagccgccg atttcagagg atacctcaaa
     5101 aattgagaat gccatatatg ctgtcgagaa aagtctgggc attatcgagg gctataatac
     5161 accaccgtcg tcagaaaatg tcgatttaga aaagtgtcag aaattggttt ccgatattat
     5221 cgctctcgaa accgaaaaac aaaatctgaa atcgaaaatt gatgagaaaa acatctactt
     5281 aaactggtat aaccgctggg gcataatttc cgaaaaagat gtggctgaac taaaagaaca
     5341 caggatatac ctgcatttat atgtaattga tcgtagtata ttgaaaaata taccggaaga
     5401 agcggccgtc gaaatattaa aagaggaaaa aaatcaacta ctaatagctt ttattgcccg
     5461 atctgaggat gtacgtcttg atctcaaaga ggagcaaatc ccaccggaag attttgacac
     5521 ggtcacacgg gaattacaga attttacttc ccgattagcc gaaatcgaac agcaacttac
     5581 tgagctggct aaaaaggctg gtttaattcg cgcctatcag caggaattgg aaaagcatct
     5641 ggaattcgcc cgcgtccgat cgggaatgca ggatgcagaa gagatcacct atttgcaagg
     5701 atattgcccc tcggatgatg tgtcccaatt gatgcaggcg gccagtgtcg agggttgggg
     5761 cattttgagt gatgatccgg atgagaccga taatccgccc actttattaa aaatgaataa
     5821 aatagctcgg ctaatccaac cggttttaga tttcctcggc actgtgcccg gttatcggga
     5881 atatgatatt agccagtatt ttttaatttt ctttgcgcta ttcgtagcaa tgattatcgg
     5941 cgatgccggt tatggtttga tttttttcct ggtagctata tttgcccatt ataaactggt
     6001 aaagcaaggt aagtccttgc cgctcttttt aaagttgctc tacctgctta gtatatgtac
     6061 tatcggatgg ggcactataa ccggcaactg gtttggatct attcagattg ccgggttgcc
     6121 attattaaag gctttgacga tacctcagct ggcaactttt ccggaattat ttcccgacct
     6181 gacggtcaat ccccaggatc aaattatgtt tatttgtttt accgtcggtg taacgcaact
     6241 ggggctagct aatattatga attttatcaa taacttgccg catttaaaag cgctgggtaa
     6301 tctgggatgg tttggggtaa cggtaggagc ctttctgttg gtattaaatt tagttttggg
     6361 atcgccgttg ccttcattta cggtgcctct gattggctgt agtgtagtgg caatagtttt
     6421 atttatgcgc caggagagtg gtaccagctt cttgaaagga atcgcgcggg gattagggga
     6481 cggatttgcg acttttttga acgttatcag ttcgttttca aatattattt cctatatacg
     6541 gctcttcgct gtgggtatgg cgaccgtggc aattgctacc agttttaacc agattgccgc
     6601 atcgttattg aaaggtttta ctttaccggc tggaatgttg atattaatta tcggacatgg
     6661 actgaacatc gttatgagta ttctatcagt aattgtgcat ggtattcggt taaatgtcct
     6721 cgaattttcc agccaattag gtatggaatg gtctggctat aaatatgagc cattcagaga
     6781 aaaaataaaa aatttaacag aaagggaaat acaatgaata ttggattgat cggtccggca
     6841 gccgctttag gattagccgc tcttggctcg gttctgggat gcggtatagc cgcccaaact
     6901 gccgtcggag gctggaagaa atgcttcgcc aacaacaagc cggcgccttt tatgctggtt
     6961 gcttttgccg gtgcgccact aacgcagaca atttatgggt ttcttttgat gctttttatc
     7021 aatggaagtg atcaagagcc tttggttaag ctctgtgcgg gtgtttttgg gggtttggca
     7081 attggttttt cggctttttt tcagggtaaa gttgccgccc gcgccgcgga tgcactggct
     7141 gaaacgggta aagggacagc caattatttt atggtaattg gtatcgtaga gactattgct
     7201 gtgttcgttt tagttttcat gttgctgaac ttataaatcg gtgcttagaa tcactacgct
     7261 ttagtcggct ggtaattata gaatctcagc cagttgctgg tagttcgaag aaggtctaaa
     7321 aagttttact aaaataaatt gataatgtat tagggaaatt aannnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnaagaaggt ctaaaaagtt ttactaaaat aaattgataa
     7501 tgtattaggg aaattaactc tctgagagcg cctttagtga aaagggctct gataatattt
     7561 tacagaagtt tttactttta ccgggagatg tgtttaataa aagaaagccg ggcttgaata
     7621 cttctgatta aataaccagc tacatattgg aaacctcctg agagttcaat atattctttt
     7681 tgatgaacga ttattcgatt aatcgcttga aaaatttaaa aactggtagc aacttcaggg
     7741 ggacagaatg ccaagcctaa catttctcgg tgcgaccggt acggtaaccg gttcacgttt
     7801 tttgttaaaa actgcccata agcaatatct aattgattgc ggtttatttc aaggtttaaa
     7861 agagttacgt ttacggaact gggaaccgtt tcctatttcg ccggaagata ttgatgctgt
     7921 gatccttacg catgctcata ttgaccatac cggttatttg ccacggctgg tacattatgg
     7981 ttttaagggg acaatatatg ccacaacggc tacaactgac ctttgccaaa tattgttacc
     8041 tgattcggct catttgcaag aagaagatgc ggaatatgcc aataaaaaac atttttccaa
     8101 acacagtcca gccttgccac tttacactac ggaagatgct ctgaaagctc tggaattatt
     8161 taaaccgatt gcctatggta gtcgtttaca attggatgat ggagtttctc taacctttca
     8221 agatgctggt catattctgg ggtcttcttt tattgatctg cgtttgccta ttgacggtga
     8281 agaacgacgt ttgttatttt ccggtgattt aggacgacca aatcaaccta ttttacgcga
     8341 tccgcatacg gtttttggtg ccgattatct aattgtcgag tcaacttacg gcgatcgctt
     8401 gcacggtaat gaagacccga aagaagtaat ggcgcgcata atcaacgaaa gtatccgacg
     8461 cggcggagtg ttgctgatac catcttttgc cgtaggtcgg actcaagagt tactttatac
     8521 aatccgtgaa ttggaagaag ctaatcgaat cccgcattta ccaatctata tggacagccc
     8581 cttagccatc aaggctacga aaatattttc taaaaacaag caggatttcg atcttaaatc
     8641 taaaacttta gaattaaagg gcaagaatgt attagaaacg gctcaattgc actttgcgca
     8701 aacccaggaa gagtccaaag ccataaataa aataaaaagc aatgccatca tcatttctgc
     8761 cagcggtatg gctaccggtg gcagaatcct gcaccatctt ttcaatcgcc tacccgatcc
     8821 gaaaaatacc gtcctattta ttggctacca ggcagttggc acgcgtggtc gcacgatcct
     8881 cgatggcgcc gaaagtgtca agattcacgg gcaatatgtc ccgattcggg cgcatattga
     8941 aagtacatcc tgcttttcag cccacgccga ttaccacgag attttagcct ggctatcgaa
     9001 ttttaattct gtgccacgaa aagtttttat tgtccatggc gaaccggaac ctgcccgtag
     9061 tttgagtgac aaaatcaacc ggcatttcgg ctggtcaacc gaattgccgg aatacctgag
     9121 ccaatatcag atcagctaaa atgtggtata attaggcaag gaaaaatatc aatggatttg
     9181 ttcgaagcga atccgcaaaa tacaaatttg ttaccgccct tagcggagcg aatgcgtccc
     9241 cgacgccttg aggattttaa agggcaagag gaaatcgtgg cgcagggcaa aattttgcgg
     9301 aaagctttag aatcccggaa aattttttcg atgattctat ggggacctcc aggcagtgga
     9361 aaaactactt tagccaaaat aattgctaat tatgtggatg ccgattttta ccagatcagt
     9421 gccgtttcag ccggagtgaa ggatgtgcgg gctattattg ataaagcggc ttataatcgt
     9481 agtcgcaatc gctcgacctt attgtttatt gatgaaattc accgcttcaa caaagctcaa
     9541 caggatgctc tcctccatgc cattgaagat ggatcgttaa ttctaatcgg agccacgacc
     9601 gaaaacccct cttttgaagt gatctcgccg ctcatctcgc ggtgccgggt gttgaagctg
     9661 gagggattat ctgatgaaaa tctggcaagt attatcaata cggctattgc taaagacatt
     9721 attctattaa agtataaaat taaatttgag ccgggtgctc aggatttttt gataaaaaat
     9781 gtcaatggcg atgctcgcaa attgttgaat gcgatcgaaa tttgccagca gatagcggcg
     9841 cccgaggaag gcgctgaatt tactatcact attgatttaa ttaaagaagc actccagcaa
     9901 agaaggcagt tatacgataa gactggtgat tatcactatg atactatttc ggctttcatc
     9961 aaaagcgtgc gcggcagtga tccggatgcc gccgtttact ggatggcttt gatgttagaa
    10021 ggtggtgagg acccgctatt catcgctcgc cgtttgatca tactggcaag tgaagatata
    10081 ggcaatgccg atccgtcggc tttgccgtta gcgaccgctg gttttcaggc tgtgcatgct
    10141 atcggtatgc ccgaagccgc tattgttctt gcccaggtca cgacctatct ggcatccacg
    10201 ttcaaaagta acgcttccta catggcatta gaaaatgccc gccaggcact caaggaaggc
    10261 agtgcgccca gcgttcccct gcatttgcgc aatgcgccaa ccaaattaat gaaagcccaa
    10321 ggttatcata agggatatcg ttatccccat agttatccag accattttgt gaaagagaat
    10381 tatttccccg gtgatggact ggcgccgacg ttttatcaac cgaccagtat cggtcgtgaa
    10441 aaattcctac gcgagcgcct tgagcagctc tggcctgagc gttaccgaag gacgaaaaat
    10501 tcctgaaata attttattta tctatacaat ttttcgcttg ggattattaa ataattatct
    10561 gtgaatatca gatatttgga tctaaaaata ataatttata tctctgatga acataacaac
    10621 cattgggata tttattttga cgttaaatag gctatttaat accagatgaa gcctacaaat
    10681 aagtataatt gtaaaaaaag cttgattatt tctaatagtt aatttatttt gagatgtaat
    10741 ttacaaaacg ctataggggc gatgtaatga gaagaaaaat agttagagtt ggcgccagcc
    10801 tatgtgtgct attacccaaa gaggtagtta gagctatggg atgggatttc gaagatgaaa
    10861 tcaatttgat cctagacgag gaaaatgaag tggtaattct ccgcaacctg caaaatcagc
    10921 caatcgagaa aaaaacgatg atggagcatt tcgacaagtt tctcgaaaat tacggaaagg
    10981 agctggatac tctggttgcc gatccggagc ctcaaatcca ataattaggg gaaaaaagat
    11041 gaaaaggaaa ataattcggg tgggttccag taaaggagtc ttactacctc gcgaggtgac
    11101 taaggcgatg gattggggtt ttggttccga aatcgagctt agtgttgatg aagagaagca
    11161 cgaggtttat ttgaagagtt taaaagtcag cgtgccgcaa gattatgaga tagaccacct
    11221 gcgacagctc gaggaagtat tggaagatta tgctgatttg ttggagggta tcgatgactg
    11281 aagaacaaat caggcaaatt aagttgatat cttatgctaa ctggttggta accgagaaat
    11341 ttggcgctga aaaaggcatc cgagacatca aggctctaac tgcggctgta accttaccgg
    11401 ggaatggaac cctttcctgc gttctgggtg aacgtctttc caatcgcaag gatttgtatg
    11461 cccagctcgg ttggtttgtt tattatatga tcgaaggcga gccgtttaat agcaagaatc
    11521 gcacaacggt gcttctaatt ttattatggg tattaaaata ttacggcctc gaattcaatg
    11581 ccgaagaact cgccaactat attaaaaccc tcgatcctct gaagcatggc aattccgaca
    11641 tttccctctg gttctcgact cattgtcgtt aaatcactta cttttttctg ctaaaaattc
    11701 cgctcaaata atcttgtcct ctgtaatggg tatataatta atttttttat aaaagtcacg
    11761 atggatgaaa ccgttaagaa accaatagcg gtaaaaaatt gggctcataa agctggaaga
    11821 gttagttttc tattccttgg ttcattaccg gatagtaaaa ttttatcgag taatactttt
    11881 gacggctcgt ttaaaaacct taacttttgt taaatgatta gtgataatac ttggttttat
    11941 ttaaaatatt atcctttgtg caacttgatg aataatccta atatcgtcct gcatttgata
    12001 accgagaagt gaattttaga tgcggcaggt agttgatttc ggctttgcct ggaactggga
    12061 atatgattgg gattttgcca atttattaga agcgaattgc cagtcggaag gattaaattt
    12121 tcttcaagtg accccagaaa agatcaaatc ggtactgact gaattagggc aaggtggaat
    12181 ttatttacaa gcattatggg atagagcggc tgacaccgat agccgattta tagaactgat
    12241 aaactgggct aagaataaca atattctcca gattaatcct cacgaaaaag ccgtccgagc
    12301 ctggaacaaa gccacgatgc acctggaatt tatcacagcc ggagtcaata caccttatac
    12361 gatagttttg gacccttata atgtccgacc gatattgccc gagattgact tgagtgtcct
    12421 aggcggtcgc tttataatca aaccggcgca cggcggcggc ggcttgggtg taattctgga
    12481 cgcgaccagt ctggagcgcg tattgactgc tcgtcgagaa aatccggatg atttttatct
    12541 tttacaggct gtaatcgaac cgttacaggt caacggacgg ccggcttggt ttcgggtcat
    12601 ttatggcttc ggaaaatgct atttttgctg gtgggatgta aagacgcata tttatacccc
    12661 tttttcgaat aatgataaag agagcctgcc aatgccatta ctaaacgata taatcgagaa
    12721 aattgccgat gttacgggat tggatgtttt ctcctccgaa attgctctga cggcagaagg
    12781 taaatttgtg gtggtagatt atattaacga tccagttgat ttgcgtttgc aatccagagc
    12841 ggcagatggt gtcccggacg aaatcgtccg agcgatttca tcggattgcg ttgccactgt
    12901 aaaaagagct gttcaggcaa aagcaactta tgaaaaattg gaaaattaga gaattggata
    12961 agttgatatt taattcaatc cagaaaattc taactgctta tttatgggtt accaattttt
    13021 aattacgaga aatattttgg ctttattatg actatcatct aaattaacgt aattaaaaaa
    13081 aggagccttt aaagccaaac cgtgagttgg gaaaggcaaa agagaaacca gaaatccacc
    13141 cagagcggtg gtttttttat ttcaggagta taaactatgg aatttagagt taaagcccgt
    13201 ctggcggacg agcagggagt aaatcgcacg attacgcgat tagcccatga aattatcgag
    13261 aattgcaaag gaaccgaaaa tgtcggaatc attggtattc gcacgcgtgg tgattattta
    13321 gccaaacgtt tagcccataa aatcgaagag atcgaaaatg tcaaactacc agtcggtgtc
    13381 cttgatgtcg ttatgtatcg ggatgatttt agaatgaaaa ccaaattgcc gcgcgttgag
    13441 gtcaccgata ttcctttcga tgtggacggc aaaatcctaa tcctggtaga tgatgtaatt
    13501 tataccggca gaacaattcg cgccgctctg gatgctctga tggatttcgg gcgaccggcg
    13561 gcaattcaat tggcagtgct ggtggaccgc ggtcatcgcg aactaccgat caaacccgat
    13621 tatgttggga aaaacatacc tacttcgatt ggtgaagaag ttcgcgtaat gattaaagaa
    13681 atcgacggta gagatgaaat cctgttggtg gaggaacaga aatgaatttg agtattaagc
    13741 atttactcgg cctggaaggt gtgccgcgcg atgacatcag taccattctg gacaccgctt
    13801 tttcattccg ggaagttcta gagcgcccga ttaagcgtgt tccgactctg cagggcaaga
    13861 ccattgtcaa tcttttcttc gagaattcca ccaggacgcg catatcattt gaactagctg
    13921 agaagcgtct atcggccgat acggtcaatt tttcatccag cggaagtagc ctcgccaagg
    13981 gcgaaagcct taaagatact gtccgcaacc tctatgctat gaaaacagat gctgtcgtga
    14041 tgcgacacgg cgtgcccggc tcagccaaac tactgacgca attcatagat gctgttgtaa
    14101 tcaatgccgg tgacggaact catgaacatc cgacccaagc ccttctggat atgatgtcat
    14161 tgcaggaaaa attcggttca ctcgacggcc tgaatatcgc aatcatcggt gacattcgcc
    14221 acagccgagt agcgctctct aatatttacg gattgttaac tatgggagct aatgtcgcac
    14281 tctgcggacc accgacgttt atgccggtcg gaatcgaatc tctgggcgta aaagtaatct
    14341 atgatctgga cgaagcttta gcctttgccg atgctatcaa catcctgcgc attcagcggg
    14401 aacggcaagg taaaggtatg atcccatcac tacgcgaata tcgagcggtt tttggcatca
    14461 cgcgcgaacg cctggataaa ttgcataaaa cgattacgat actacatccc ggcccgatta
    14521 atcgcggagt ggaaattgac tctgacgtcg ccgatagcga gcactcactt atccttcatc
    14581 aggtgctaaa cggcgtcgcc gtgcgtatgg cagtactttt tctattattg ggtggcaaat
    14641 aaatggaatt atttaggagt aaaaatgcaa attaaagcca ttaacgaaga actactttta
    14701 cagaatgcgg tgatcgttga tccattcaaa gataacattt atgaggcgga cattctgatt
    14761 ggaaaaggta aaataaaaaa tattgagcaa aagattgacc cgaaaaatgt aaaaatatgt
    14821 gaagacctca agggatcata cgtcgcgccg ggatttattg acctgcatac tcatctacgc
    14881 gagccgggca atgaagatga ggagacaatt gcaaccggtg tcagagccgc gctggctggc
    14941 ggattcacta aaatttgctg tatgccgaac accgatccgc ccattgatac tcaagaagcc
    15001 gtacgtttta tctaccggca agccgaaaac ctgttggcgg aagtttatcc gattgcggca
    15061 atcaccaaag gtcgtcaggg cgccgagctg accgaaatgg tcgaattagc taatgctggt
    15121 gcggtggctt tctccgatga cggcgctccc ttgaaaaacg cccagattat gcgctatgct
    15181 ctcgaatatt cccgattgac cggcaaacca attatcaacc atgccgaaga cattgattta
    15241 aaagccgatg gtttgatgaa tgaaggcgtt gtctcgactc atctgggatt accgggtaat
    15301 cctgctatcg ctgaggaaat aatgattaat cgcgatttgc aattggcgaa atttacctca
    15361 gcgcgtttac acgttccgca tgtctcgacc gccggttccg tggaattgat ccgccaagct
    15421 aaagccgaag gtgtacacgt gaccgctgaa gttactccgc atcatttttc cttgacagac
    15481 gaatatatgc gcacttttga tgccaatggc aaagtcgctc cacctctgcg aaccgaagcc
    15541 gatcgtcagg ctttgattgc cgggcttaag gacggcacga ttgacgctat cgctaccgac
    15601 catgcgcccc atacgattga cagcaaagaa acttcgttag acctcgcatc gtatgggatg
    15661 atcggattgg aaaccgcttt tgcgctggct atgaccaatt tggtacatcc ggggcatttg
    15721 actcttttgc aattgataaa attgctgacg gtcaatcccg ccgcgataat gaacatcgcc
    15781 ctgccgaaat tagaaaacgg cgtggacgct aatctgacga ttttcgatcc caaccagtgg
    15841 tgggttttca atcgtaaaaa tatttattcg cgctctcaga atacaccttt tatcggggcg
    15901 gaactgaccg gacggatcaa agcggtcgtc accaaatcgc attacatttc attataagta
    15961 ctctcaccaa acctaaagcg aaattcctga aagttgagga tacgcttaat cttatttaaa
    16021 ttaattgtcg aatgacgtca gacatcaaat cgcataaaaa acaatcagcg ctatcaacct
    16081 ctcaaacagt gatagccttc caaaagaaag tccggaagca tttccacgga aattacctgc
    16141 tccatttgcc gcccggttat gccgaatctc aaaatgactg gccaacgatc ttcttcctac
    16201 acggctccgg tgaacgaggg aacgatcccg aaatcgtccg gcgccaggga ctcccccaag
    16261 ttattgataa aaaaccagat tttccgttca tcgtaatctc gcctcaatgt cccgccaatc
    16321 gctggtggtc gctggaatgg ctggacgcac tattcgatga agtggtcaag ctctatcgaa
    16381 ttgatcagaa acgtatttat ttaaccggat tgagtatggg cggctttgct acctgggact
    16441 gggctatcga acacccggaa cgttttgcgg cgatagcgcc gatttgcggc ggtggcaatc
    16501 cgctcgaagc cgacaaaata aaagatgttc cgacctgggt ttttcatggt gccaaagatg
    16561 aaatcgtgcc gcttcaaaaa tcacaagaga tggttgcggc gctgaaagct ggcggcggaa
    16621 aagtgaaatt tactgtttat ccgaacaccg gtcacgacgc ctggacgaag acttataata
    16681 atcttaggct ctacgaatgg tttctaaagc agacaaaaaa tgactgactg gatcgcaata
    16741 aaaaaagatg agaagcatat tgctcgcgaa aaagcgaaag ccagagaatt acgccaatcc
    16801 aactggtgga aacagaaaat agcggccggg atatgctatt actgcggcaa gaaatttaag
    16861 ccgcaggaat taacgatgga tcatatcgta ccgattgccc gcggcggaaa aagcatcaaa
    16921 ag
//
LOCUS       DAOMJU010000041        16736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_3322_length_16736_cov_23.236017, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000041 DAOMJU010000000
VERSION     DAOMJU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 16736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16736
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3322_length_16736_cov_23.236017"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..486)
                     /locus_tag="PLO58_05465"
     CDS             complement(52..486)
                     /locus_tag="PLO58_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74273.1"
                     /translation="MKQDEQQNDKRTLLFLRRLIQNVIGFLQRNYKLLITWAIIIIAV
                     IVGIHFRVDKGIIVVLVMIFGLFAEAFSGLIALLATVPVIGPIVAKVLTLPIFWIFNA
                     LGYFVSLVAIKRGYKKEVINYRFVTMVFLTGFAVGFIIAKLL"
     gene            complement(521..1315)
                     /locus_tag="PLO58_05470"
     CDS             complement(521..1315)
                     /locus_tag="PLO58_05470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQO74274.1"
                     /translation="MLLAIDIGNTNATVGLFQENDLKGCLRINSHPVRGADYYGRRIE
                     SLLKKQGIAINVIDGIVIGSVVPRLTEPFKQMAQTFFHIEPLLINCNLNLGCCLLVDN
                     PAEVGADRICNAVATGVKYHCPSIVVDLGTATTFDVLDKEGNYIGGAIAPGLITGARD
                     LFRKAAQLYPINLTFPPRVIGKNTTAHMQSGILFGHLAMIEGMIRRIGVEINQPDIFV
                     IATGGFSNVIAQQSPLIQASDPYLTLDGLRLIYGMNLPQTSKSQSR"
     gene            complement(1318..2100)
                     /locus_tag="PLO58_05475"
     CDS             complement(1318..2100)
                     /locus_tag="PLO58_05475"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQO74275.1"
                     /translation="MNSFRLVNLPIPTEILKFPVVHFAEIDSTNAYLLGRHSHPSGTI
                     VWADFQSAGRGRLGRSWITPPNEALLFSIYLKIHHNETPLFIYSFLTAVGVLEGLQTL
                     VTPAWLALKWPNDVLLKNMKVCGILVQSKTSSGDNADVVIGIGVNLNQNSDFFIPDLP
                     FAGSLYSITGKKYEREDVLAKLIFAIDQNLVALNGNGTVPILEKWRKYCPYIGQVIKV
                     NDGQEIHSGLFYDISADGGLILQVGRTYRTFHAAEVTIVRET"
     gene            complement(2105..4258)
                     /locus_tag="PLO58_05480"
     CDS             complement(2105..4258)
                     /locus_tag="PLO58_05480"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQO74276.1"
                     /translation="MSNIKRLFFLATCLLTLNFRLLAAEGMYPPDQIPTKIKGLKVKP
                     ADIYQPDGGGLAGAVLLFGGGTGSFVSADGLVLTNHHVAFSAIQKNSTDEQNYLAQGF
                     YARTKAEELPAPGYEASLMIGFEDVTEKILKSFTPQMTPEERTRALERAIAQLEAESE
                     DSANGLTGKVVAMLDGASYYLYHYRKFRDIRLVYAPPQSIGNFGGEVDNWMWPRHTGD
                     FSFMRIYCAPDGKPAEYSEKNVPYQPKAFLPISTRGLNPDELVFILGYPGRTYRNVTS
                     YSVAYNQNLVYPLRIRIFQEIINELEDESQKSPEVDLLLSSRLKGFYNGLKNNQGLLA
                     GFKSENILGQKKLVEKELVQKIAGKPAWQEQYGNILPEIQKAYDEYYTGFERDMYIEY
                     LRYVTVLADALTIEKWSREKAKPESEREYGFFDYQIARTKRDFKVRRIGYYAPADARI
                     LKLLLNHLAEFSSAERPAFLRELIQDQPADSAKAVIAAYVDSAFAHTRLTDADECLAM
                     FDLSAESLLDRQDPLITLAVAVNGELERIEKDQKTLSGKMLILKPQYLSALKNVLGKT
                     VLPDANRSLRFTYGNVEGYQPRDAVFYQPQTTLAGVIAKHSGIEPFNVPERLIELANS
                     RDYGRWIDRELNDVPVAFLSSCDITGGNSGSPVLNARGELVGCAFDGNWEALTNDWQY
                     NPALSRAISVDVLYVLFILDKYSSADELIEELELR"
     gene            complement(4283..5035)
                     /locus_tag="PLO58_05485"
     CDS             complement(4283..5035)
                     /locus_tag="PLO58_05485"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HQO74277.1"
                     /translation="MGKLAGTIIGGGIGWALFGPLGALIGAYIGNVVTNESGVQKKRG
                     KAEPGATFATYGDTRAGDFAVAMLSLFTYISKSDRNVKSSEIQYVKKFLVDQFGTRNA
                     QDLLYLYREILGKDYNIQDIAHQIRDNMDYYSRMELLHVLFGIAAVDGDLHTAESQAI
                     REISDALGISARDYETIRAIFSGSANRSYEILGIKPDDDVEIIKKAYRDLAVKYHPDK
                     VANLGPEFQKLAEEKFKSINDAYQTIRKERGF"
     gene            complement(5164..6285)
                     /locus_tag="PLO58_05490"
     CDS             complement(5164..6285)
                     /locus_tag="PLO58_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74278.1"
                     /translation="MRRTLSRIVVLTLFAVATIMAQEFSMDQIKELAATNGKLYLQPL
                     ATGFGTAMNSGLYNTAGTHGTLGFDIQLKTGVGLAPASKASQTTYQFDLSALDDIAFN
                     IENNSQSYSITLNPEEIFSDCTTPNILGGEAEPFRVNQPYVQGEIESQLATQLRAQYP
                     LLTDAQINAMIAQMNPEIEDAINNIQVKSIPGMKSIKALEDLKTVPFPFPILQASVGL
                     PMGIEPSIRIIPKFKIPGDIGEAIGEISALGGGLKLDVDRWIPIPMFPLDIAVQGYYQ
                     QMEIGDLLSANSTAFNLMVSKTILMLTPYVGVGKESSKFTASYKMEDGTKIKFDLKGK
                     NDTRFTAGLNIKIAVISINAEYSMGEYNAAGLGVGFALR"
     gene            complement(6353..>8164)
                     /locus_tag="PLO58_05495"
     CDS             complement(6353..>8164)
                     /locus_tag="PLO58_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQO74279.1"
                     /translation="DQNQDSCRQADELCWRQDINQSLKSDSTLIVTGKITAERAGRSL
                     YLAELEYSLDENLDDNYAGTDLVVAFPAGCLTLNEFLAYPTSNQVEFIECINVSDETI
                     DLSGWMLGNRYLKAEISGGKVPAGGYLILSGDSACFNYFKTDNAAVIIPGKWPGLNNS
                     ADQIWLKDLSGKIIDSLYYGAGWPIKAGYSAEKILPHLASDTSQSWALSVSPEHQTAG
                     YFNSVTRPENDLSLDSLQLSAITGDTNTVFAGCYFISNKGQNQACGANLIISLENDFT
                     SQIPLDCIGPGQADSGNFEIGPLTSGKHTVTARVNWPADQINDNNMLLRSIQISWSRG
                     SVLLSEFMPYPWDIRIASSSIAEYLEFYNSSDSAIDLAGWMISDQNTAVRRSIPPGYF
                     LPPGEYFVIAGDSSVLNFPDLAPSQTWITAKFPSLSNSEDAVYLYDPTGRSIDSLFYN
                     STWPIIQGIAMERISFDNSNRAMNWRASVAAAGGTPGQPNSVILPEKLDKLGIKPTAD
                     VFSPDGDGIDDEIGIQYLLPFPSAKVTIEIYDLVGRLIYRLATNLETAASGVIYWDGA
                     SDYSPKARVGIYIVRCLANDIASAKSVEYITTLVLAR"
     assembly_gap    8165..8264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8265..10494)
                     /locus_tag="PLO58_05500"
     CDS             complement(<8265..10494)
                     /locus_tag="PLO58_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQO74280.1"
                     /translation="MNLAKATAAVLFIIGVFITGLDGQALTINEVMADPVGSDYYDEF
                     IELYNYGDLAVTLSGYYLEINGYVDTLSFVADYGDVLLPHSFALIMDRGYLVSNKSSS
                     YEDLIPENTLLVTIQDSSLARTGLINTGASQIYLFNAQGDTISAVLTTAKPLSGYSWE
                     KIIPEGTNQISNWGNSRNQRGTPGFWNSIAPRNYDVAIEGLKVVSPVDQIFPHQPIDV
                     QFTVKNFGLKAATSVQIICGLDQNSDRQIDEIFYDEMIALAAGDSAMKNATIPGLSSG
                     IYTILAEAIYAADERYDNNLDSLDIKILFSENCLIINEFMYYPATDGGEWVELLNISA
                     DTINLKKWMLSDNSTTIMLTGQDFYLPPQKYLVIVNDSTTFISYWGQVPFILDCPKTM
                     PALNNTKDSIIIRDHSAHLIDGLEYSSAWGYRQGVSLERKNSFASSNQASNWSLSTDL
                     DKATPGRRNSIDLRDYDLALDSVKVKPGKSPFQHNSPVTAQIYLRNNGKKPADSFKLN
                     IAIINSFLGTVSDTTITIFSPLQSNESQTVAVTFNAIPGGLHQISAEILWSADETLTN
                     NIANCPLAVGYPTGVLVINEIMFVPLAGQCEWFEIYNRSEITLDLNGWSFRDAMGKRL
                     ALADSGIVIKAGEYAVVAARSDFTLDYPDFQRLLIVPTSFPTLNNTTDSLFLYDATGN
                     IIDIICYRDSWGGGTGISLERLDPAQPALNADNWGGSLIIATPGQTNSIIKLDNDLAI
                     VPS"
     gene            10661..12376
                     /locus_tag="PLO58_05505"
     CDS             10661..12376
                     /locus_tag="PLO58_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74281.1"
                     /translation="MKKLIFGKFAFLKKTERFLLMLGLFGTLLSINLNAQSDRYTRKS
                     VAFLDALMVTDSRIRLNPEDEKYLLAAIHNRIRIARFDYNPLPDIVRYTFKEQLRARG
                     AVSESELVDLIEATIVPEIVKILDIEKEIRAQNLVDETQRNSFISLKAKELGITADQI
                     EQVMNASYIYLPFLTSYTVSKPKDDPNLTVTIKGGLFWYHIVPGDDPYLESIVTLHTE
                     ASGSAEKAKSDAESEAFRLAASTFAINLQVLTRDIEMFKLSAPIAAIEKRTVKFPLGT
                     AEGIKLDDPFFVGEYIQNSGDKITFKESGFVRVSKVGDNRQDPRQLSAAYAIQKGDWV
                     KGMTMVEHPRLGVDVALKPRGFFMNINEGVFESEDFLIFFDDYDGFAFGADLDFQINI
                     APLTNKRQSFLVAGATAGVVPVKSELYSTDTFIDWIIPDANNSASLVYGYTGYMRRFY
                     FGPVAIHGEALIGIQHLLLTDKYNEKDVTISNNSLGGRLNLALEYALNIDSNIGIFAG
                     FQAFPSFDWWTIKYGDEEIDVANYADTDKPSISSLSPTFGIYFHYSPPTLSFNPASAF
                     QANGN"
     gene            12394..13671
                     /locus_tag="PLO58_05510"
     CDS             12394..13671
                     /locus_tag="PLO58_05510"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HQO74282.1"
                     /translation="MKKLSVTILTIFCIASLVFAKSEKSKTKSNLPRSHEAVILESTS
                     PSEVMVRATGYSSEGGKAAEMDKEAEADARKAAVWLILLGGTDPLLQTDAEKAEFAKI
                     QEEFFAPSNIKQFIVWESDYYHSRLKIDGGKTLKIEKTFKVNKSLIHDYLVERGILTK
                     TAAITEALGLPTIMVIPETDGKQAPIELLRTNPNLKKGAEVIEAYLTARKFEVIVPEQ
                     QQALQELTSAHYTLQGASEDYSYLLALSIGSDIYITYNVAVESRLLGSSTVKKGVVGC
                     RAYETTTGRLLGTETGYSPERTAPDAVLIEEAMNDAVDKVISRIMAYWKEDIQQGVQY
                     KVILNISDSFTKSEAEDMIYAFGDLLKKASKRIKENVMADYSYDVLIWCDPALYQSSS
                     DLYRFFKQNYKGQGRLERVSLNRKLILLNIVEE"
     gene            13696..15051
                     /locus_tag="PLO58_05515"
     CDS             13696..15051
                     /locus_tag="PLO58_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HQO74283.1"
                     /translation="MTKIILVLAGLAITSASAQVPDWYKTNQHKKYPASRYFLGVAVA
                     DTKAEAIELARADVAKQIQVRIESELETIESEFREGDKNYLKAETTNRTKSIVAETVS
                     GIEIAEVKEVKGKTYVLAVLNKEKYLSTLEIQMDEILMKSEQLIASARQMVADGRILN
                     ALNNFIDAQNTIPDFYTKSSLYTALSGRKYPNFNQFTGPGIVAEVREILAHIELKILS
                     GNNQIAVAGQNLPTPIKVQVVYVRDGEIIGVAGFPVIARYDGGEIITKIETDANGIAE
                     FNCVATPTDNVGQSGAVKIALDLSKIPEQYRDAMTRSAVQINYILTANDLKFTVEVIQ
                     KNGQHLTFIEDEVVRLIAANGYTCSADAPWLVQATLVIISQKEVPSPAGNQHLVEAQL
                     QLRLIEKQTGNVFAALDINTKGLDSQSPDSALSKAAQNLKIPSNKFAAFLQSANQPKN
                     D"
     gene            15079..15633
                     /locus_tag="PLO58_05520"
     CDS             15079..15633
                     /locus_tag="PLO58_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HQO74284.1"
                     /translation="MKKLLVFVLLISVALFTVNCGGKQPKAKMDDIPEWYLNPPQAED
                     AIYQAGDAVKSSMALAKDAADARARDAIARTVELRVTNMMKDFMQQSGMGEDAEALEF
                     TSSVSKQVANTVLKGCRIVKREMKVEGNNYHAYSLAEYNLNSLIEETLNEARRQKALY
                     NEAKANMSFEELEREIEKLKNVGQ"
     gene            15685..16428
                     /locus_tag="PLO58_05525"
     CDS             15685..16428
                     /locus_tag="PLO58_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74285.1"
                     /translation="MKFCLFTIALVLGLKILAFAQECVPSWFEKLPEVEGVRLAVGYS
                     GKFTNEAGAKSAAVQLAHKNMTKQISIRLQFDIEELADGRIRLLNPSFKEFYEGSILN
                     AVESNSSVIDSTITVDGYFVLLALPAIEYLPIPNACDKPWNTRPDWIENLPQEDGYVY
                     GIGIVARYRNWVRAWQDADEYARFDLGKNLQVNTESIHAVQRNNRTTIESVILKQTYD
                     LTINDATIVARWLDTANGVYYSLCRAPRP"
BASE COUNT         4785 a         3777 c         3576 g         4498 t
ORIGIN      
        1 ctgagaaaat gtagattgag ccggtagcac gaccagatgt aaataggttt actataatag
       61 cttggcaatt ataaaaccga cggcgaatcc ggtcaggaaa accattgtca caaagcgata
      121 gttgatgacc tcctttttat agcctctctt gattgccacc aaggaaacaa aataacctaa
      181 cgcattaaaa atccaaaaaa tcggcagggt taatacttta gccacaatcg gtccaattac
      241 aggcacagtt gccagcagag caattaagcc ggaaaaagcc tcggcaaata atccgaaaat
      301 cataaccagc acaactataa tacctttatc aacccgaaag tggattccta caataacggc
      361 aatgatgatt atcgcccagg taatcaataa cttgtaattc cgctggagaa agccgatgac
      421 attttgtatc aagcgcctca aaaacaaaag ggttcttttg tcattttgtt gctcgtcttg
      481 tttcatattt ttaccataaa attgtttata aagcctaagg ttacctcgac tgggatttcg
      541 acgtctgagg taggttcatc ccgtaaatca ggcgcaagcc atccagtgtt aaatagggat
      601 cgctggcttg gattaaggga gattgttgag caataacatt gctgaatcca ccggtggcga
      661 ttacaaaaat gtctggctga ttaatctcta ctccgatacg acgtatcatt ccttcaatca
      721 tagctagatg accaaacaaa atcccactct gcatatgagc ggttgtattc ttaccaataa
      781 cgcgcggtgg aaaagtgaga tttattggat agagctgggc ggcttttcta aaaaggtccc
      841 tcgcaccggt tataagtccg ggagcaatcg ctccgccgat ataatttcct tccttatcca
      901 gcacatcgaa agtcgttgcc gtgcccagat ccacgacgat gctcggacaa tgatatttca
      961 ctccagtggc tacggcattg caaatgcgat cagccccaac ttcagccgga ttatcaacta
     1021 aaagacaaca tccgagattc aaattgcaat tgattaggag cggttcaata tggaaaaagg
     1081 tttgcgccat ttgcttaaac ggttcggtta ggcgcggtac gactgagcca attacaatcc
     1141 catcaattac attaatggca atgccttgct ttttcaacaa agattcaatc cgccgaccat
     1201 aataatctgc tcctcgaacc ggatgactat ttattcgcag acatccctta aggtcatttt
     1261 cctgaaatag tcccacggtt gcattggtat tacctatatc aattgctaat aacattccta
     1321 agtctccctg acgattgtta cctcagcggc atggaacgtc cgatatgtcc gaccgacttg
     1381 tagaatgaga ccaccgtcgg cactgatatc atagaacaaa ccactgtgaa tctcttgacc
     1441 gtcgttgact ttgattacct gcccgatata cggacaatat ttacgccatt tttccagaat
     1501 cggaacagtt ccgttaccat tcagcgcgac caaattttga tcaatcgcaa aaatcaattt
     1561 ggcgagcaca tcctctcttt catatttttt tccggtaata gaataaagcg aaccggcaaa
     1621 cggcaaatcg gggataaaaa aatcactatt ttgattcaaa tttacgccaa ttccaattac
     1681 cacatccgca ttatcccctg agctggtttt actttgcacc aaaataccac aaactttcat
     1741 gttctttagt aaaacatcat tgggccattt cagtgccaac caggctgggg taactagagt
     1801 ttgtagtcct tccagaacac ccaccgcagt tagaaatgag taaataaaaa gcggtgtctc
     1861 attgtggtga atttttaaat aaatagaaaa tagtaaagcc tcgttgggtg gagtaatcca
     1921 gctacgtccc aaacgccctc gcccagcact ttgaaaatca gcccagacta tcgtcccaga
     1981 aggatgcgaa tgccgaccca gtaggtaagc attggtagag tctatctctg caaaatgaac
     2041 caccggaaat ttgagtattt cagtaggaat cggcaggttg acgagccgga aagaattcat
     2101 aactttagcg caattcaagc tcttcaatca actcatcggc ggaactatat ttatccaaaa
     2161 taaaaagaac atagagaacg tcaacgctaa tagcacgcga aagagctgga ttatattgcc
     2221 aatcattggt cagcgcttcc cagttgccat cgaaagcaca gccgactaat tctccccgcg
     2281 cgtttagaac cggactgcca gagttgccgc cggtgatatc gcaggagctc agaaaagcca
     2341 ccggaacgtc atttaattcc cgatcaatcc agcggccata atcgcggcta ttcgccagct
     2401 cgatcaatct ttctggaacg ttaaagggtt caataccgct atgtttggca attactccgg
     2461 ctaaagtggt ctggggttga taaaagacgg cgtcgcgcgg ctgatagcct tcgacgttgc
     2521 cataagtaaa ccgcaaactg cggttggcgt cgggcaaaac cgtcttaccc aaaacatttt
     2581 tcaaggccga aagatactgc ggcttaagaa ttaacatttt gccgcttaat gttttctggt
     2641 ctttctcgat gcgctccagt tcgccgttga ctgcgacggc taaagttatc aatggatcct
     2701 gccgatcgag taaagactcg gcgctcaaat caaacattgc cagacattcg tcggcatcgg
     2761 taagtcgggt atgagcaaag gcactatcaa cataagcggc gataacggct ttagcagaat
     2821 cggcaggctg atcctgaatc aattcgcgca aaaaagcagg gcgctcagcc gaagaaaatt
     2881 cggctaaatg atttagtaat aatttcaaga tacgggcatc agccggggca tagtagccga
     2941 tgcgcctgac cttaaaatca cgcttggtcc gagcaatttg gtaatcaaaa aaaccgtatt
     3001 cacgttcact ttccggttta gccttttcac gactccattt ttcgatagtt aacgcatccg
     3061 ccagaaccgt aacgtaacgt aaatattcaa tgtacatatc gcgctcgaat ccggtataat
     3121 attcatcgta ggctttctgg atttctggca gaatattccc atattgttct tgccaagcag
     3181 gcttgccggc tattttttga actaattcct tctcaactaa ctttttttgt cccaaaatat
     3241 tttcactctt gaaaccagct aataaaccct gattattttt cagcccattg taaaaacctt
     3301 ttagtcgtga agataggagt aagtcaacct ccggactttt ttggctttca tcttcaagtt
     3361 cgttgatgat ttcttgaaag atccgaatgc gtagcggata tactaaattc tgattataag
     3421 ccacggagta actcgtgaca ttgcgatagg tgcgacccgg atagcccaaa atgaaaacaa
     3481 gctcatcggg attaagtcct ctggtagaaa tcggtagaaa agctttaggc tggtagggta
     3541 catttttctc gctatattcg gccggcttgc cgtcgggcgc gcagtaaatt cgcataaagg
     3601 aaaagtcacc agtgtggcgt ggccacatcc agttatcaac ctcgccacca aaattaccga
     3661 tagattgcgg aggggcataa accaggcgaa tgtcccgaaa ttttcggtaa tggtaaagat
     3721 aataactggc accatcgagc atagcgacga cctttcctgt cagtccattg gcagaatctt
     3781 cgctctcggc ttctaattgg gcgatagcgc gttccaaagc gcgagtgcgt tcttccggcg
     3841 tcatttgcgg ggtaaaggat ttaagtattt tttcagtgac atcttcaaaa ccaatcatta
     3901 gcgacgcctc atagccgggc gccggcagtt cctcggcttt agtgcgggca tagaaacctt
     3961 gtgccaagta attctgctca tcggtcgaat ttttctgaat ggcactgaaa gcaacatgat
     4021 gattagtaag cactaaacca tctgccgaca caaatgaacc ggttccgcca ccgaataata
     4081 gcaccgctcc ggctaaaccg ccaccgtccg gttgatatat gtcggcgggt ttaactttca
     4141 agcctttgat tttggtcggg atttgatccg gcggatacat accttccgcg gctaatagtc
     4201 tgaaattcaa tgtcaaaaga caagtcgcca gaaaaaagag tcgttttatg ttactcatag
     4261 tttctttctc tctttttaat tgttaaaagc cacgttcttt gcgaatcgtt tgataagcgt
     4321 cgttgatgga tttgaatttt tcttccgcca gtttttgaaa ttcagggcca agattagcta
     4381 ctttatccgg gtgatatttc accgccaaat cgcggtaagc tttcttaatt atttcaacgt
     4441 cgtcatccgg tttaattccc aaaatctcat aagatcggtt ggcgctacca ctgaaaatcg
     4501 cccgaatagt ttcatagtca cgggcggaaa ttcctaaagc atccgaaatt tcccgaatcg
     4561 cttgcgattc tgccgtatgg agatcaccgt ccacagcagc aattccaaat aagacatgaa
     4621 gcagttccat ccgggagtag taatccatat tatcgcgaat ctgatgggca atgtcttgga
     4681 tattataatc tttaccaagt atctcccgat acaggtagag taaatcctga gcattccgag
     4741 ttccaaattg atcaactaga aatttcttta cgtattgaat ttcagaggat ttgacattcc
     4801 ggtcggattt actgatatag gtaaataacg agagcattgc caccgcaaaa tctccggcgc
     4861 gagtatcgcc gtaagtcgca aaggttgcac ccggctcggc tttacctcgt tttttctgga
     4921 ctccagattc gttggtaaca acattaccga tatacgcccc gattaaggct cccagtggtc
     4981 cgaaaagcgc ccagccgatt ccaccaccaa ttattgttcc agctaatttt cccatattct
     5041 tgatctcaat ttaaaatatt taactttaga aataataaat attccttgga taaaatccaa
     5101 cattaaataa aaatccctcc acctatgtaa gagtggaggg atcataacta tttggtataa
     5161 atactatctt aaagcaaaac cgacacccag accggcggca ttgtattctc ccatactata
     5221 ttcggcattg atagatataa ccgctatttt gatattcaag ccggcggtaa aacgcgtatc
     5281 attcttgcct tttaaatcga atttgatctt cgtgccgtct tccattttat aagaggcagt
     5341 gaatttactg gactctttgc caaccccaac atacggcgtt agcataagaa tagtcttact
     5401 gaccattaaa ttgaaagccg tgctattggc gctcaatagg tcaccgattt ccatttgttg
     5461 ataataacct tgaacggcaa tatctaatgg gaacatcggt atcggaatcc agcgatcaac
     5521 atccaatttt aaaccaccgc caagagccga gatttcgcca atagcttcac cgatatcgcc
     5581 aggaatttta aatttcggga ttattcggat agacggttcg atgcccatcg gcaatccaac
     5641 gctagcttgc aagatcggga atggaaatgg tacagtcttt aagtcttcca gagctttgat
     5701 tgatttcatc ccaggaatag atttcacttg aatattattt atggcatcct ctatttcagg
     5761 attcatctgt gcaatcatag cgtttatctg agcgtcggtc aaaagaggat attgggctct
     5821 taattgagtt gcaagctgag attcgatttc tccctggacg taaggttggt taaccctgaa
     5881 aggctccgct tccccgccaa ggatattcgg tgtagtacag tcagaaaaaa tctcttcagg
     5941 attcaaagta attgaatagc tctgcgaatt attctcaata ttgaacgcga tatcgtccaa
     6001 tgccgaaaga tcaaattgat aggtagtctg actggctttg ctggcgggtg ctaaacccac
     6061 tccagtcttt aattgaatat caaatcccaa agtgccgtgc gtcccagcag tgttatatag
     6121 accggagttc atcgctgtcc caaacccggt ggctaacggt tgtagatata gcttcccgtt
     6181 agtagcggcc agttctttaa tctgatccat tgagaattcc tgtgccataa tcgtggctac
     6241 cgcaaaaagg gtcaatacaa caatccggct aagtgttctt ctcatatttt cctccatgat
     6301 ttcatttttg ctttaatgtt agggtcattt ttagtaaaaa tcaagtagaa ttttagcgag
     6361 ccaatactaa agtcgtgata tactccacag atttggcgct ggcaatatca ttcgctaagc
     6421 atcgaacgat ataaattcca acgcgcgctt ttgggctata atcactagcc ccatcccagt
     6481 aaattacgcc gctcgccgct gtttcgaggt tcgtagccaa tcggtaaatc aggcgtccca
     6541 ccagatcata tatctctatg gtaacttttg ctgaaggaaa aggtagtaaa tattggattc
     6601 cgatttcatc atcaatccca tcaccgtctg gcgaaaatac atctgccgta ggtttaatgc
     6661 caagtttatc aagcttttca ggcagaatca cactgttagg ttgcccgggt gtaccgccgg
     6721 cggcggcgac acttgcgcgc caattcatcg cccgattaga gttatcaaat gaaatacgct
     6781 ccattgctat cccttggata attggccagg ttgaattata gaaaagactg tcaattgacc
     6841 gaccggtcgg atcatataag taaacggcat cttcactatt actcaaggat ggaaatttag
     6901 cggttatcca agtctgactt ggtgctaaat ccggaaagtt cagcaccgat gaatctccag
     6961 caattacaaa atattccccc ggcggcagaa aataaccggg cggaatactc cggcgtacgg
     7021 cggtattctg gtcggaaatc atccaaccgg cgagatcaat tgcgctgtcg ctggaattgt
     7081 agaattcaag atattcagca atcgaactcg acgctatccg aatatcccaa ggatatggca
     7141 taaattcaga tagaagtacg cttcctcttg accaggaaat ctgaatcgaa cgcaatagca
     7201 tattattatc gttaatctgg tctgcaggcc aattaacgcg ggctgtgaca gtgtgtttgc
     7261 cggaggttag cggaccaatt tcgaaatttc cactgtcagc ttgcccggga ccaatacagt
     7321 ccagtggaat ctgagaagtg aaatcattct ctaaagatat tattaaattt gcaccacagg
     7381 cttggttctg ccctttattg gatatgaaat agcacccggc gaaaacagta ttcgtgtcgc
     7441 cggtaatcgc ggacagctgt aatgaatcga gacttaaatc attttccggt cgggtaacgc
     7501 tattgaaata tccggcggtc tgatgttcgg gactgaccga aagcgcccac gattgggagg
     7561 tgtcacttgc caggtgtggc aggatttttt cagccgaata accggctttt attggccagc
     7621 cggctccata atagagcgaa tcaataattt ttccactcag atccttcaac caaatttgat
     7681 cagcggaatt attcagaccc ggccatttcc ccgggataat gaccgcagca ttgtccgtct
     7741 taaaataatt gaaacaggcg ctgtcgccgc ttaagattaa atatccacca gccggaactt
     7801 ttccacccga aatttcagct tttaaatagc gattacccag catccagccg gataggtcaa
     7861 tcgtttcatc gctgacatta atgcactcaa tgaactcaac ctgattgctg gtcgggtaag
     7921 ctaaaaattc gttgagggtt aaacagccgg ctggaaaagc taccactaaa tcggtaccag
     7981 cataattatc atcgagattc tcatctaagc tatattcaag ttctgccaaa taaagacttc
     8041 gcccggctct ctcggcagtt atcttccccg taacgatcag ggtcgagtcc gacttaaggc
     8101 tctggtttat atcctgtcgc caacaaagtt catctgcttg tctgcaacta tcctgatttt
     8161 gatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgatg gcacaatggc
     8281 taagtcatta tccagtttga taatggaatt agtctggccc ggcgtggcta ttatcagcga
     8341 accgccccaa ttatcggcat tcaaagccgg ttgcgctgga tcaagtcgtt ccagcgagat
     8401 gcctgttccc ccgccccagc tatcccgata acaaataatg tcaataatat taccggtagc
     8461 atcatagaga aaaagtgaat cagtcgtgtt attcagggta ggaaaagagg tcggtactat
     8521 cagcaatcgc tgaaagtccg gatagtccag ggtaaaatca gatcgcgccg ctacgacggc
     8581 gtattcgccg gctttaatca ctatgcccga atcagccaaa gccaggcgtt tacccattgc
     8641 atcgcgaaag ctccagccat tcagatcaag tgttatttct gaacggttat agatttcgaa
     8701 ccactcgcat tgaccagcaa gtgggacaaa cataatttca ttaatgacta aaacgccggt
     8761 cggatagcca accgccagtg ggcaattggc aatattatta gtcaacgtct catctgccga
     8821 ccacagaatt tcggctgaaa tttggtgcaa gcctcccgga atagcattga aagtaaccgc
     8881 gactgtttgg ctttcattcg actgcaaggg agaaaaaatc gtgatagttg tatcggaaac
     8941 tgtccccaag aacgaattaa ttatggcgat atttaactta aatgagtcgg caggtttttt
     9001 accgttattg cgcaggtaaa tttgagcggt gactggtgaa ttgtgttgaa acggcgattt
     9061 gccaggtttt actttaaccg aatcaagtgc gagatcataa tcccgcaagt ctattgaatt
     9121 tctccgaccg ggagtagctt tatccaggtc tgttgagagc gaccagttac tagcttgatt
     9181 gctactcgca aatgaatttt ttctctcaag tgatacgccc tgacgataac cccaggcact
     9241 cgaatattct aatccatcaa ttagatgagc gctgtggtct cggattataa tagaatcttt
     9301 ggtgttattc agcgccggca tagtcttagg acaatccagg ataaatggca cttgtcccca
     9361 ataactgatg aaagtggttg agtcattaac aatcacgagg tatttttgtg gcggcagata
     9421 aaaatcttgg ccagttagca ttatggtcgt actattgtcg ctcagcatcc attttttcag
     9481 attgatcgta tcggcggaaa tattcaaaag ctccacccac tcgccgccgt cagttgccgg
     9541 ataatacata aattcattga ttatcagaca attttctgaa aacaaaattt taatatccaa
     9601 tgagtcaaga ttattatcat accgctcatc cgccgcataa atagcttctg ccaaaatagt
     9661 gtaaattccc gacgacaaac ccggtatcgt cgcgtttttc attgcggaat ctccagctgc
     9721 aagcgctatc atctcatcgt aaaatatttc atctatttgc cggtcggaat tttgatccag
     9781 tccacagatg atctgaacag aggtagcggc tttcaagccg aaatttttga cagtgaactg
     9841 cacatcaatc ggctgatggg gaaaaatttg atcaactgga gaaaccactt tcaatccttc
     9901 aatggctaca tcataattgc ggggcgcaat gctgttccag aaacccggtg tgccgcgctg
     9961 gttgcgacta ttgccccagt tagaaatttg attagtaccc tctggaatga ttttttccca
    10021 gctgtagccg gacaggggtt tggcagtcgt tagtacggct gatattgtat ctccctgagc
    10081 gttaaaaagg tatatttgac tggcacccgt atttatcagg ccggtgcgtg ccaaggaact
    10141 atcctggatt gtcaccagca gggtgttttc aggaatcagg tcttcgtaag agctcgattt
    10201 attactaact aaatagccgc gatccataat gagagcgaaa ctatgcggca aaagaacatc
    10261 accataatcg gcgacaaaac tcaacgtatc tacatagccg tttatttcca ggtagtaacc
    10321 gctaagggta acagccagat caccataatt gtacaactca ataaattcat catagtagtc
    10381 tgacccgact ggatcagcca tcacttcgtt aattgtaagg gcttgaccat ccaaaccggt
    10441 gataaacacg ccgattatga aaagcaccgc ggctgttgcc tttgctaaat tcaaaaactc
    10501 tccaacttct tgccgttatt ttatgtctta aatcacagaa agtcaatgat aaattcatta
    10561 agtatttcct cgcttgtaaa aaaagttgat tttcagattt atttttaaga aaattaagag
    10621 tgttcgattt ttaacaaata gaaaatttat ggagggaagt gtgaaaaaat taatttttgg
    10681 caaattcgca tttttaaaga aaacggagcg cttcttattg atgcttgggc tgttcgggac
    10741 attattgtca attaatctta atgcccaaag tgatcgttat acccgcaaat cggtggcgtt
    10801 tctggatgca ttgatggtta ctgattccag aattcgcctc aatccggagg acgaaaaata
    10861 cttgttggcc gcgattcata acaggatccg gattgcccgc tttgattata accctttgcc
    10921 ggatattgtg cggtacactt tcaaagagca attgcgggcc agaggtgccg tctccgaatc
    10981 ggaacttgtt gacctaatcg aggctaccat agttccggaa attgtaaaaa tactcgatat
    11041 agagaaggaa atccgcgccc aaaatttggt ggacgagacc cagcgcaatt cgtttatttc
    11101 gctcaaagcc aaagaattgg gtattaccgc ggaccaaatc gaacaagtaa tgaacgcttc
    11161 gtacatctac cttccgtttc ttacgagcta tacagtcagc aaacccaaag atgacccaaa
    11221 cctgacggta acaatcaaag gtggtctgtt ctggtatcat attgttccgg gcgatgatcc
    11281 gtatttggag agcatcgtga ctctacacac cgaagcctcc ggttccgcgg aaaaagctaa
    11341 aagcgatgct gaatccgaag cttttcggct tgccgccagc acttttgcca ttaacctgca
    11401 ggttttgacc cgcgatattg aaatgtttaa gctgagcgct ccgatagccg ctatcgaaaa
    11461 gcgcaccgtg aaatttccgc tcggcacagc ggaaggcatt aaactagacg accctttctt
    11521 tgtcggtgaa tatatccaga acagcggcga taaaatcact tttaaggaat cagggtttgt
    11581 gcgcgtcagc aaagttggcg ataatcgtca agatccgcga caactttctg cggcttatgc
    11641 tattcaaaag ggagattggg taaaaggaat gacaatggtt gaacatcccc ggttaggtgt
    11701 tgacgtggct ttaaagccgc gcgggttttt tatgaatatc aacgaaggtg tttttgaaag
    11761 cgaggatttt ttaatctttt ttgatgatta tgacggtttc gccttcggcg ccgacctgga
    11821 ttttcagatc aatattgctc cattaactaa caaacgtcaa tcatttctgg ttgcaggcgc
    11881 cactgccgga gtagtgccag ttaaaagtga attatatagc actgatactt ttattgattg
    11941 gataatcccg gacgccaata attcggctag cttggtttac ggctacaccg gttatatgcg
    12001 acggttttat tttgggccgg tggcaattca cggcgaagcc ttaattggca ttcaacattt
    12061 acttttaacc gataagtaca atgaaaaaga cgttacgatt tcaaataata gtctcggggg
    12121 gcgcctgaat ctggcactcg aatatgccct taatattgat agcaatatcg gcatttttgc
    12181 cggttttcag gcttttccgt catttgactg gtggacgatt aaatacggag acgaagaaat
    12241 tgatgtcgct aactatgctg atactgacaa gccctctatt tccagcttgt caccaacttt
    12301 cggcatttat ttccattatt cgccgccgac tttatcattt aacccagcat cagcgttcca
    12361 agccaatggt aactaaacta aacgaggagt aaaatgaaaa aattatcggt aacaatatta
    12421 actatttttt gtattgctag tttagttttc gccaaatctg agaaatcaaa gacgaaatca
    12481 aatcttccgc gttcgcacga ggctgtcatt ttagaaagca cttcaccttc tgaggtaatg
    12541 gtgcgtgcca ccggttacag ttccgagggc ggcaaagccg ccgaaatgga taaagaagcc
    12601 gaagccgatg ctcgtaaagc cgccgtctgg ttgatcttat taggaggtac cgacccactt
    12661 ttgcagaccg atgccgaaaa agctgaattc gctaaaatcc aggaagaatt tttcgcgccc
    12721 tccaacataa aacaattcat cgtttgggaa tccgattatt accattctcg cttgaaaatt
    12781 gacggcggta aaaccttgaa gatcgaaaaa actttcaaag tcaacaaaag cctaatccac
    12841 gattatttgg tagaaagagg tattcttacc aagactgccg ctattactga agcgctaggc
    12901 ttgccgacga taatggttat cccggagaca gacggtaaac aagcaccaat cgaacttttg
    12961 agaaccaatc cgaacctcaa aaaaggtgcg gaagtaatcg aagcctactt gacggctcgc
    13021 aaattcgagg taattgtacc cgaacaacag caggcgctcc aggaattaac ttccgcacat
    13081 tacaccctcc aaggagccag cgaggattac agttacctgc tggcactctc aattggtagt
    13141 gatatttata ttacctataa tgtcgccgtc gaatctcgcc tattaggttc atcaacagta
    13201 aaaaagggtg tggttggttg tcgtgcctat gaaactacga ctggtcgctt gctcggcacc
    13261 gaaactggct atagtccgga aagaaccgcg ccggatgccg ttttgattga agaagctatg
    13321 aatgatgccg tagataaagt catcagccgg attatggctt attggaaaga ggatattcag
    13381 caaggagtgc aatataaagt gattttgaac atcagtgatt ccttcaccaa atctgaagca
    13441 gaagatatga tttacgcttt tggtgatctg ttgaaaaagg ctagcaaaag gattaaggaa
    13501 aatgtaatgg ctgattattc ctatgacgtt ttgatatggt gtgatcctgc tctttaccaa
    13561 tcctcatccg atttgtatcg ttttttcaaa caaaattata aaggccaggg tcgccttgag
    13621 cgggtcagtt tgaaccgcaa actaatcttg ttaaacatcg tcgaagaata agaggtcatc
    13681 gtgcaaaggc aatcattgac taaaataata ttagttttag caggtttggc gattacatcg
    13741 gcatcggctc aggttccgga ttggtacaaa accaaccagc ataaaaaata tcccgccagc
    13801 cgctatttcc tgggcgttgc cgtggcagat actaaagccg aagccatcga actagcgcgg
    13861 gctgacgtcg ctaagcagat tcaggtgcgt atcgagtctg aactcgaaac catcgagagc
    13921 gaattcagag aaggcgataa aaattactta aaagccgaaa ctaccaatcg cactaaatcc
    13981 attgtcgcgg aaaccgtctc gggaatcgaa atcgccgagg tcaaagaggt caagggcaaa
    14041 acctacgtcc tcgccgtgct caataaggaa aagtatctct ccactttaga aatccagatg
    14101 gatgagatcc tgatgaaaag cgagcaactt atcgcgagcg cacgccagat ggtcgccgac
    14161 ggtcgaattc taaacgcgct gaataatttc attgatgccc agaatacaat cccggatttc
    14221 tatacaaaaa gttcattgta caccgccctt tccggtcgca agtatcccaa tttcaaccag
    14281 ttcaccggac cgggaatagt tgcggaagtg cgtgaaattt tagcgcatat cgaattaaaa
    14341 attctgtcgg gaaataatca aattgccgtc gccggtcaga atttgcccac acccattaaa
    14401 gtgcaggttg tctatgttcg cgacggcgaa atcatcggag tcgccggttt tcctgttata
    14461 gctagatatg atggcggtga aattattact aaaatcgaaa ctgacgcgaa cggaattgct
    14521 gaatttaatt gcgtagctac tccaaccgac aatgtcggcc aaagcggggc ggttaaaatc
    14581 gccctagatt tatcgaaaat cccggaacag tatcgcgatg cgatgactcg ctccgcagtt
    14641 cagatcaact acatcctgac tgctaatgat ctaaaattta cggtcgaggt tattcagaaa
    14701 aacggccaac acctgacatt catcgaagac gaggtagtgc gattgatcgc cgccaatggc
    14761 tatacttgtt cagcggatgc gccctggcta gttcaagcaa ctttggttat tatctctcag
    14821 aaggaggttc cctcgccggc tggcaatcag cacttggtag aagctcagtt acaattacgc
    14881 ttgatagaaa agcaaactgg caacgttttt gccgcactgg atattaatac taaaggactc
    14941 gattctcagt cgcccgatag tgcgctctcc aaagccgctc aaaatctgaa gattccaagt
    15001 aataaatttg ccgctttttt acaaagcgcc aatcagccca aaaatgatta attccaaaaa
    15061 gtaacaaaag gagaaagtat gaagaagtta ctcgtatttg ttttgttaat ttccgtagcc
    15121 ttgttcacgg tcaattgtgg cggtaaacag cctaaagcca aaatggatga tatccctgaa
    15181 tggtatctca atccgcctca agctgaagat gccatctatc aagccggcga tgcggttaag
    15241 tccagcatgg ccctcgccaa agacgcggct gatgctcgcg ctcgcgacgc tattgcccgg
    15301 acagtggaac tcagagtcac caacatgatg aaagacttta tgcaacaaag tggtatggga
    15361 gaggatgccg aagccctcga attcacttct tccgttagca aacaggtcgc caataccgtc
    15421 ctgaaaggtt gccggattgt caagcgggaa atgaaggtcg aaggcaataa ctaccacgct
    15481 tattctctcg ccgagtataa cctgaactca ttgattgagg aaactcttaa cgaagcccgg
    15541 cggcaaaaag ctctttataa tgaagccaaa gccaatatga gcttcgaaga actggaaaga
    15601 gaaatcgaaa aacttaaaaa cgtcggtcaa tgattgaacc gttaaagccc gtttggctct
    15661 aaccagacgg gctttttttt ctcaatgaaa ttttgccttt ttactattgc cctggtcctc
    15721 gggctaaaaa ttctggcgtt tgctcaagaa tgtgtccctt cttggtttga gaaattgccc
    15781 gaagtagaag gagtccgtct ggcagtcgga tattcgggta aattcaccaa cgaagccggc
    15841 gctaaatccg ccgccgtcca attggctcac aaaaatatga ctaagcaaat ttccattcga
    15901 ctgcaatttg acatcgagga actggctgat ggacgaatac gcctgttgaa tccatctttt
    15961 aaagaattct atgaaggttc aattttaaat gcagttgaaa gtaactcttc cgttattgac
    16021 tcaacaataa ccgtggacgg ttatttcgtt ttattggcat tgccagcaat cgaatatctg
    16081 cccattccca atgcttgtga caagccctgg aatacacgac cggactggat tgaaaatctt
    16141 ccccaagaag atggctatgt ctatggaatt ggtattgtcg cccgataccg caattgggtg
    16201 cgcgcctggc aagacgccga tgaatacgcc cgcttcgatc tcggcaagaa tttgcaggtt
    16261 aatactgagt caatccacgc cgtccaacgt aacaatcgca ccacgattga atcggtgatt
    16321 ttgaaacaga cctatgatct gacaatcaat gatgctacga tcgtcgctcg ctggttagat
    16381 actgctaacg gtgtctatta ttcgctctgc cgagcaccca gaccttgagg ggcattggtc
    16441 attagtcatt ggtcattagt cattggtcat tggtcactgg tcattagtaa agaggctaag
    16501 taaaaaggag cacccaaaat ctgaccattc aatttactta cggcattaag aacatcaccg
    16561 gctgggaagc gaatggtcat tagtcattgg tcattggtca ctggtcatta gtaaaggcta
    16621 aggctgagaa aagaggttga gaaaagagaa tggtcattag tcattggtca ttagtcatta
    16681 gttggcaatt tgcacaacat cgtgaacaca aaattagcat aacatcgtgg acaatt
//
LOCUS       DAOMJU010000042        16438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_3418_length_16438_cov_20.168467, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000042 DAOMJU010000000
VERSION     DAOMJU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 16438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16438
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3418_length_16438_cov_20.168467"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1597
                     /locus_tag="PLO58_05530"
     CDS             164..1597
                     /locus_tag="PLO58_05530"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQO74286.1"
                     /translation="MHRFQLFSLIIFWASISNCSNPISPKPENPFKNINLQILSDSTL
                     AITWQGEFESEALLSLDRKVGEASWQMDYTNVPANDSIYVDTLYSVHTDTVYSYRLRL
                     KESDNSSSISPSVALLSERCVPVDFSARQIDKSTIRIAWRDQSFGEAGFRIDRQVGND
                     DWQIAYAKADSNSYEYFDITTTSSEISYRIAAYAGSSFSSFTEIAKIKLGSLTLANLY
                     FGTEPTFEVMTWNIEEFPRFDTLTVSAVSQAIKALDIDIIGMQEIKSGTAFQQLLDSL
                     DTWDGYRTNSAAYGINLAFVYNPANINVNQYYEILEHEDAFPRPPLVIELEWQNVPII
                     VINNHFKAMGDEDSQNRRLQACTLLEEYIRTNLADDNVIVIGDLNDELTDAQSVNVFG
                     PFLSQPSAYKFADYSIAQGSSKYWSYPTWPSHIDHILITDELFGVFANSASVIKTLLI
                     ENYMEGGWNEYAVKISDHRPVALKLAF"
     gene            1686..2027
                     /locus_tag="PLO58_05535"
     CDS             1686..2027
                     /locus_tag="PLO58_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74287.1"
                     /translation="MLNIIKSFVAILKIEIEDLAADVELMMNECQKKKDAGKITDYVY
                     LENMALYRHELLAVRSFARLLESTELSNFETLDALITYLQESFREAGYVRAINICVER
                     KMKKVAGYVSR"
     gene            2266..3351
                     /locus_tag="PLO58_05540"
     CDS             2266..3351
                     /locus_tag="PLO58_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQO74288.1"
                     /translation="MKLLDRYILKKFLLIFGLCLLAFLVIFNIVDLIEKLDKFLKADM
                     PSNLIALFYLYQLPYYLNLALPMALLLAAVFTIGLLSKHNELDAIKSSGISLYRVSIP
                     LLIAGILISIGSFFFEDAVVIPATRRRIDIEAHNMRRRKYIPKTFFTNIMYQDSPNCN
                     IVIGNFSTKDNIAQNVTIQYNDQFRLVRRIDARKMVWLPDQQLWQLFDYKVRSFDQDG
                     TEIVAAASSDTLIQLNLQPEDIVKSDLNPEGMRYRELAYFIKRLQESGNDARKWEVNK
                     HFKLAFPFTNFIVILFGVPLAAAKPHKGIAFGAGMSLLAIFIYYGFIKLGQVLGYKGI
                     LSPLLAVWLGNIVFLIAGGYLLYKIRQ"
     gene            complement(3588..4943)
                     /locus_tag="PLO58_05545"
     CDS             complement(3588..4943)
                     /locus_tag="PLO58_05545"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HQO74289.1"
                     /translation="MGWLNNRYKITLLLMLGMSIMATAQTRRDPRALALAGAYGSLAR
                     GIFAIDYNPANLAIPHEYHSYRIFGGFGTNVSTNFLSIKQYQKYNGKDLEAGNGHLKK
                     EFMNDIPDEGWRVFTDIYFPLPLISYSTYNRAISSDLIVIGDLGLPKDLLNLVFDENP
                     IGETLDLDFNEEVMAVIQWGYSLAFPVGNIFLGFTAKYLQGLAYIGLNPDSSYGSVTT
                     YFEPERTYIDGYGQYYFQQSLGGRGFALDIGLTTEELNGYRLGLSLTNLFGSITWNKS
                     TLLSRSVSAEKVLPWEGNFYKYVFSIDEARFDKFFGKASVDDVFHGEGYKIKDTTAFR
                     VKYPSLVRFSAAKILEENITLASDLVVGFEDRLYSFGAWKWCLGLESTKSQRFPLRVG
                     LSFGGHSSRELTFGSGFHWGLIHLDWALGFNHGLWPTTTTGLNFNMLCYSTKRANTSY
                     R"
     gene            complement(4987..7245)
                     /locus_tag="PLO58_05550"
     CDS             complement(4987..7245)
                     /locus_tag="PLO58_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74290.1"
                     /translation="MIWILSFIEGLFLLKHFRLYSTIFFLSIGLICGCSYEPLAPPEM
                     PTYYQTINLPLADVSLPLADLVNPDNNIYGDSTKDQLYFQFSGNLDTVTLTENIFQIP
                     AVGNVNFSQNFSELSESQLTFSRSVTQTTKLSEIITIPGILPSPNDLLIGEIARYELD
                     GQRYKYRVFDKDDVPYFERVDYLTVGSGTFQTEIDNQLPVAMDSVRITLRNKNGSLIA
                     ESFYENVPAGATLRDGTPGNLNGAQLFDSIEVIVTAVMAGSQGQPVTIPANTDPFIAI
                     KFDTNIEEIESFTGIPKPIVTTQNQLLPPSNNTIFKATIAQTTTSPLDTNFLDLTIHN
                     LMPFNLRMQVIFHNFYIRNEPLTIDTIVQSGLTVENPARLDGYIFRNPDPTTVVDSFL
                     VEIIATILPNAGEQVVTIPTDMGDASIDVNLYFARLKLDMLEGFFNESFDIPPMAIAN
                     IPTGLANVDFGQVFLKLTFFNEIQARTNVSLVLRGLKKGVAPQTITEDGSIAKASPAN
                     LIQESMMEIDIAPIFNMVPDSILVSGQAIIPSDDTSRLQIGKAFWGFYEITVPFQVKI
                     GPVTFIPVNSTEMAAIDPETRQKIRDGLIKSSIYTEIINDFPFSGNLSILMSNYDYFP
                     LEPDSLDAGYFWLNDTLYARTDTGDVPIIIDTLLLVELPPPEAFNSNGSVKTPGFTLA
                     ETPLDSAKMEAILRDETHYIRPRVHFNATKDFVIVGYYDEVRILAAISLTVETMELIN
                     NNNENETPATVP"
     gene            complement(7261..>8510)
                     /locus_tag="PLO58_05555"
     CDS             complement(7261..>8510)
                     /locus_tag="PLO58_05555"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HQO74291.1"
                     /translation="NLGLTAQYRTYVYLLGINGYVTNNFYSLESGARFSEKNLTADGG
                     ELQREFLEKLPKGGYRLNSSVASPLPLVNFSVGNKAFTTSLIQISDYYLSKPALDVIF
                     GNWEKGTTYNLDLRCDAMTAVEYVYSIGIPFGKMSVGISLKYLQGLGYYGLDPAYSTG
                     QIAVDTSDFVLYGNGSYYFRESSHGRGYGADLGVAFEDLNGWTLGFSVLNLGESVKWN
                     TETALSKWLKDNGIDVLKMMGFQIKSFPVKNTDLKLDFEGESYFYNFRIDSLDADALF
                     RSENTYQDHFSDTKLIKQDSSAFRVQVPLVLKVGAAKQLRKDLLTAIDFSMSFSDRLN
                     LRKGWRTAVGFEYAYFPKMPLRVGLALGGISGWEFDIGSSVNLAALHIDWALGMNRGL
                     WVHSMQGFDFALATYLVRGGKKK"
     assembly_gap    8511..8610
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8611..8870)
                     /locus_tag="PLO58_05560"
     CDS             complement(<8611..8870)
                     /locus_tag="PLO58_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74292.1"
                     /translation="MKPKKLSKRLTRILRSSNWLWQLILLGFFLYGLPQPLDAQYYDS
                     PINRALGGAGNAITRGIFCVGQNPANLGLTAQYRTYVYLLGI"
     gene            complement(8873..10393)
                     /locus_tag="PLO58_05565"
     CDS             complement(8873..10393)
                     /locus_tag="PLO58_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74293.1"
                     /translation="MLDTENGNPIKADQSGVLYYSFTDSTSMPIEINPTTFRIVPAMT
                     ISQTLDLRKNIKRNVNNTVNPINYIMKKAWDDSNNRVTSGVLDNSTGFTADNIPINAL
                     VLAAQLSDSFPNPIQIRVIAPNFQKNPGQSVVATLDLPVNSLQNSIMIELGGASLIGG
                     LLKEEIDSLEFNIEVQVLGELAEATSSLKQKLTTSLTVGQLALESFNGRVFATGETPP
                     ALVAKSLTGASDIEFDSARASLNIETFGRDFDSVYVHLSGKKVHGVETVSDTMIAINR
                     ADLKMDIGEVVSNLPDTVRVFVKVMTATADFERNTVFNAGVNVKYNISVPLRFKIPPQ
                     ITLASGNTTTYFIKDSTTRARLITSQNGGELDMTVENYTQLQGDLYFLISNFNFFPFD
                     TLGGNLPSDFVIFDGTICHLGDDTTVVEIDTLAVLEFPPAQFSENTLIVPGIKSQIFQ
                     ISSDAIGLLADTCYFKPYFKLVNPDTSLATITATQSIHVRGFLDLYFVAANLKDSD"
     gene            complement(10553..11806)
                     /locus_tag="PLO58_05570"
     CDS             complement(10553..11806)
                     /locus_tag="PLO58_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQO74294.1"
                     /translation="MTGKIKDITLAPSGKLKIEWAESHMPVMMALRQKYRDSKPLKGM
                     RISGCLHVTKETAVLIRTLIAAGAEVAWSGCNPLSTQDDVAAALYEEGVPIWAWHGLT
                     TAEFYWCIEQTLLQKPNLTLDDGADLVFTIHNKFPELAAGVLGGSEETTTGVHRLRAM
                     AEDGKLLYPMVAVNDAETKWDFDNVYGTGQSSIDGILRATSILYAGKTMVVAGYGHCG
                     KGVAKRASGMGANVIVTEVKPTAALKAKLDGFRVMPMDQAAELGDIFITATGMKDVIV
                     SRHFEKMKDGAIVCNTGHYDCEINLKELATLTKTKQEMRPNCVKHELTNGRSIYVLGE
                     GRLVNLVAAEGHPSEVMDMSFANQFMTMIFLAQNGAKLAKKVYDISVEQDQEIALLKL
                     QTMGVNIDTLTPEQVRYKTAYSEGT"
     gene            complement(11806..12963)
                     /gene="metK"
                     /locus_tag="PLO58_05575"
     CDS             complement(11806..12963)
                     /gene="metK"
                     /locus_tag="PLO58_05575"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017186814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQO74295.1"
                     /translation="MKKYSFTSESVTEGHPDKICDQVSDAILDALLTVDPESRVACET
                     MTTTGLVMVVGEITTKAYVDIPRIVRETIEKIGYTDPDFGLDARSCAVITSIHEQSEN
                     IALGVNETEKHEQGAGDQGLMFGYACDETPELMPLPISMANKLTMRLAEVRKKGILNW
                     VRPDGKSQVTVEYEDDHPKRITNVVVAVHHNPEIAHEKIVADIKNHVILPVCQGYLTP
                     ETKFYINATGIFAIGGPEADSGVTGRKIICDTYGGMGRHGGGAFSGKDPSKVDRSASY
                     MARYIAKNIVAAGLARRCEVQLAYSIGVADPISVYINTSGTGKQPEERLAEAVRAIFP
                     LKPAAIIKYLCLKRPIYLKTAAYGHFGREDEEFSWEKTDKVAELKQYFGVK"
     gene            complement(13025..13999)
                     /locus_tag="PLO58_05580"
     CDS             complement(13025..13999)
                     /locus_tag="PLO58_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQO74296.1"
                     /translation="MKAIIPAAGIGERLRPLTLTRSKVLLPVAGKPIIGHIYDRLALT
                     GIKDVTVIIGYYGEQVRDYSLKNYDFNFTFIEQLEQCGLGHAVGLGLTPEDGPVMIIL
                     GDVILELDFKGLSSRSENLIGVMPVDDPRRFGIVEMKDGKVTRLVEKPSSSTSNLAIA
                     GIYLIQRGALLKKAIDYIITVNIKTKGEYQLTDALQKMLEWGESFEVINIEHNLDCGT
                     RKSLLKTNAYLLNKNPQPPPAIPETVIIPPVYIGRDAVIKRSIIGPNVHLGAEVQVMN
                     SVLSNAIISDGAILKDVILDESLIGQKSQVSGIVQQIDTADYTVQKLS"
     gene            complement(14015..14998)
                     /gene="hprK"
                     /locus_tag="PLO58_05585"
     CDS             complement(14015..14998)
                     /gene="hprK"
                     /locus_tag="PLO58_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HQO74297.1"
                     /translation="MNQNDLTVGRIYHENRKKLGFLRINGDIGHDTVVTSSKLNRPGL
                     EISGFWESFDPHRIQLIGEGEFRYIKTLSGEKVEEIFEHFFSAKIPLMIFAHDVKPPV
                     CAVRLARKYQVALLATPVTTTLLVASLLDYLDRELAPSTIIHGSLVDVYGIGLLLTGP
                     SGIGKSEIALDLVGRGHRLVADDVVKIIRRADNIIIGSGVEILEHHLELRGLGIVDVR
                     NIYGIRAIRQQKRIEVQIELEEWDNTKNFERIGAQEKYNKILDVDIPIINLPIYPGKN
                     ITVIAEVIALNHVLKIMGTNAALNFQKKIAATIRQKTQISDLQNYLKKDLE"
     gene            complement(14995..15282)
                     /gene="raiA"
                     /locus_tag="PLO58_05590"
     CDS             complement(14995..15282)
                     /gene="raiA"
                     /locus_tag="PLO58_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQO74298.1"
                     /translation="MNIELTTRHYEITDRAKKHLEEEMNNLKLYEDLITSCKVIMEKT
                     KDGESIEISIHVRGKDLIAQVASDDMIKSIDLAIAKIERQLKKFKEKRYAR"
     gene            complement(15402..16331)
                     /locus_tag="PLO58_05595"
     CDS             complement(15402..16331)
                     /locus_tag="PLO58_05595"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HQO74299.1"
                     /translation="MMINFLRFLAQERGYSENTIMAYRRDLEQFFLFYAEYANQVPID
                     LNSVSKMAIRHYLGKLSESGLEISSISRKLASLKAFFKFAARQGYADSNPAALILSPK
                     AKKRLPVVLTEEEIAKAIELPDDNTLVGKRNRAILELFYSSGIRLGELIALNVGDLNF
                     SRQTLRVFGKGAKERVVPFGKKAAEVINDYLNMRKKEVGALELNSPLFISSSGGPNSQ
                     PTGYRRISRSQVQKLISGLLQQASEQTHLSPHVLRHSFASHLLDHGADLNAVKDLLGH
                     SSLSTTQLYTHVRISTMKEVYQQAHPHADSGKK"
BASE COUNT         4664 a         3729 c         3250 g         4695 t
ORIGIN      
        1 aataaagtca tttgaattgc tttttatggc tatcctctcg ttagcaattt atccaattac
       61 tcttaaaggc ttaaaaatca accgattcga ggatcttttt cttttgttac tgtatggtgg
      121 ttatctttat aaaaattagc cttaaatcgc agtatttata gtaatgcatc gttttcagct
      181 attttcgcta atcatttttt gggctagtat ttcaaactgc tccaatccaa tttctccaaa
      241 gccagaaaat ccgttcaaaa atattaatct gcaaatatta tcggacagca ccctggcaat
      301 aacctggcaa ggagagttcg aatcagaagc actgctcagc ctcgatcgca aagtcggtga
      361 agcatcttgg caaatggatt ataccaatgt gccagctaat gattctatct atgtagatac
      421 gctatattcc gttcacactg atacggtcta ttcctaccgt ttacgtttaa aagaaagtga
      481 taattcatct tcaatttctc caagcgtagc gcttttatcg gagcgatgtg taccggtaga
      541 tttttcagcg cggcaaatag ataaatccac aatccggatt gcttggcgag accaatcctt
      601 cggtgaagcc ggtttccgaa tcgatcgcca ggttggtaat gacgattggc aaatcgctta
      661 tgccaaagcc gattccaatt cctatgaata tttcgacata accactacca gttcagagat
      721 tagctaccgg atcgccgctt atgccggatc atcattttct tctttcacag aaatagcaaa
      781 aattaaatta ggttcgctca ctttagctaa cctttatttt ggtactgaac cgacctttga
      841 ggtaatgacc tggaatatcg aggaattccc tcgttttgac acccttaccg taagcgctgt
      901 cagtcaagcc attaaagccc tcgacattga cattatcggc atgcaagaaa taaaaagtgg
      961 gacggctttt caacaattac tggattcgct cgatacttgg gacggatacc gcaccaatag
     1021 cgcggcttac ggtattaatc tagctttcgt gtataatcct gcaaatatca atgtcaatca
     1081 atattatgaa atccttgaac atgaggacgc ttttccccgc ccgccgctgg tgatagaatt
     1141 ggaatggcaa aacgttccta ttattgtcat taataatcat tttaaagcaa tgggcgatga
     1201 agattcacaa aatcgtcgtc tgcaagcctg cactttatta gaagaatata ttagaacaaa
     1261 tctcgctgat gataatgtca tcgtaatcgg cgatttaaat gatgaattga ccgatgctca
     1321 gtcagttaat gtttttgggc catttttaag ccaaccttcc gcctacaaat ttgccgatta
     1381 ctctattgct caaggtagca gtaaatactg gtcatatcca acctggccaa gtcatattga
     1441 ccacattctc atcaccgatg aattattcgg cgttttcgcc aattctgcat ccgtgattaa
     1501 aacattattg attgaaaatt atatggaagg tggctggaat gaatatgcgg ttaaaatttc
     1561 cgaccaccgc ccagtggcgc tcaagctcgc tttctaaggc ttttctcctc tacctactaa
     1621 agcacttgca attcccaata ttggttataa attaaatcgt aagattttta aaagcgggag
     1681 gtatgatgtt aaatatcata aaaagcttcg tcgctattct gaaaatcgaa atcgaagact
     1741 tagccgccga tgttgaattg atgatgaatg aatgccagaa gaaaaaagat gctggcaaaa
     1801 tcaccgatta tgtttacctg gaaaatatgg cgctctatcg gcatgaattg ctggctgtcc
     1861 gttcgtttgc ccgactgctg gaatccactg aactgagtaa tttcgaaact cttgatgccc
     1921 taatcactta tttacaagag tctttccgtg aagccgggta tgtgcgagca atcaatatct
     1981 gcgtcgaacg caaaatgaaa aaagtcgccg gttacgtatc ccggtaatca aagtttcccc
     2041 ggtttgatag acctgcggct tgtctgctat cattcaatta ccaataattt aataatctaa
     2101 atcggtgctt gcgactcaaa gccttaggtt tagttccttt ccatttcatc tgtcatcgtt
     2161 tcgtttcaag actaataatc gacttttcag gtgctcgatc ccccagcctg aaaataaaaa
     2221 tttgataaac atctgcgccg gtgataattt ttagcggagt ttgacttgaa attactcgat
     2281 cgctatatac tcaagaaatt tctactcatt ttcgggcttt gcctgcttgc ttttctggtt
     2341 attttcaata tcgttgattt aatcgaaaaa ctagataaat ttctcaaagc ggatatgccg
     2401 tccaatttga tagcgctttt ttacttgtac caattacctt attaccttaa tcttgccctt
     2461 ccaatggctt tactgttagc ggcggtattc accatcgggc tgttatcgaa acataatgag
     2521 ctagacgcca tcaaatcctc cggtatcagt ctttatcggg tttcgattcc tttactaatc
     2581 gccggaattt taattagcat cggctcgttt ttctttgaag atgccgtagt aatccctgct
     2641 acccgtcggc gaattgatat tgaggcacat aatatgcgcc gtcgtaaata tatacctaaa
     2701 actttcttta ccaacattat gtatcaggat tcaccaaact gcaacatcgt gattggcaat
     2761 ttttcgacga aagataatat cgctcagaac gtgactattc aatacaatga tcagttccgg
     2821 ctggtgcgac gcattgatgc tcgcaaaatg gtctggttgc ccgatcaaca actctggcaa
     2881 ttattcgact ataaagttcg ctccttcgac caagacggta ctgaaatcgt ggctgccgcc
     2941 tccagcgata ccttgattca gcttaattta caaccggaag atattgtcaa gagcgacctt
     3001 aatccggaag gtatgcgtta ccgcgaatta gcctatttta ttaagcgtct tcaagaaagc
     3061 ggtaatgacg ctcgtaagtg ggaagtcaat aagcatttca agttagcttt tcctttcacc
     3121 aattttattg taattctttt cggggttccc ctggcggcgg ctaaaccgca taaggggatt
     3181 gccttcggcg ctggtatgag tttattggcg atattcatct attatggatt tatcaagtta
     3241 ggtcaggtat tgggttacaa aggaatcctt agtccgcttc tcgcggtctg gctcggtaat
     3301 atagtcttcc tgatcgccgg aggttactta ctttataaaa tccgacagta gcgtaaaaac
     3361 tttaattttt cctcaactac ataatttgcc ggataataat agataatttt acgagtggat
     3421 gagtttttaa gcacttttaa gtgcatttaa gaaaattttt aacatcaggc tttttatgtt
     3481 taattgccca agggtatgat tgaatggctg aatgagtttg attttacaat aagatagcaa
     3541 ttaattgaca attgagtaat tattatagtt ctcgataatt gcgctaacta tctataggaa
     3601 gtgtttgccc ttttggtcga ataacatagc atattgaaat taagaccggt ggttgtcgtc
     3661 ggccataatc catgattaaa acccagcgcc caatccaggt gaatcaatcc ccagtggaaa
     3721 ccacttccga aggtcaattc ccgtgaactg tgcccgccaa aagacagccc tactcttagc
     3781 gggaagcgct gtgatttagt tgactctaaa cccagacacc acttccaggc gccaaacgaa
     3841 tacaaacgat cctcaaaacc aactactaaa tccgaagcca gcgttatatt ttcttccaat
     3901 attttggccg cactgaatcg taccaaactg ggatatttca cccgaaatgc ggttgtatct
     3961 ttaattttat atccttcacc atgaaagaca tcgtccacac tagcttttcc aaaaaactta
     4021 tcgaaccggg cttcatcaat actaaacaca tatttgtaaa aattaccctc ccacggtaaa
     4081 actttttccg ctgaaaccga tcgggacaat agagtgcttt tattccaggt tatagatcca
     4141 aacagattag tcaggcttaa gccgagtcgg taaccattta attcttcggt cgtcagtcca
     4201 atatccagtg caaaaccgcg cccacctaac gattgctgaa aatagtactg tccataaccg
     4261 tcaatataag tccgctcagg ttcaaaataa gtggtaaccg agccatagga agaatccgga
     4321 tttaacccga tataggcaag accttgcaga tatttagccg taaatcccag aaaaatgtta
     4381 ccgactggaa aagccagtga atagccccac tgaattacag ccattacctc ctcgttgaaa
     4441 tccaggtcga gagtttctcc gattgggttt tcgtcaaaga ccaaattcag taaatccttc
     4501 ggcaagccta agtctccaat cacgatcaag tcggagctga tcgcgcgatt atacgtcgaa
     4561 taactgatca acggtagagg aaaatatatg tcggtaaaaa cccgccagcc ttcatcggga
     4621 atgtcgttca taaattcctt ctttaaatgg ccattgcctg cttctagatc cttaccgtta
     4681 tatttttgat actgtttaat tgaaaggaag ttagttgaca cattggttcc aaatccaccg
     4741 aaaatacgat atgaatggta ttcatgggga atagccagat tagccggatt ataatctatg
     4801 gcaaaaatac cgcgggctaa cgagccataa gctccggcta gtgctaaagc gcgtggatcg
     4861 cgtcgcgtct gagccgttgc cataatgctc atcccgagca tcagtaacag agtgatttta
     4921 tacctattgt ttaaccagcc catttttttt cttcgccaac caaccagctg tgaaatcgta
     4981 aatgtactac ggaacagttg caggcgtttc attttcatta ttgttattaa tcagttccat
     5041 cgtctcaact gtcagcgata tcgccgccag aattcttacc tcatcataat aaccaacaat
     5101 cacaaaatct ttggttgcat taaaatgcac tcgcggtcga atgtaatgag tttcgtctcg
     5161 taaaattgct tccattttag ccgaatcaag gggcgtttcg gcaagggtaa agccgggagt
     5221 ttttacactt ccattactgt taaaagcctc cggcggcggc aattcgacta acagcaaggt
     5281 atcaattatt atcggaacat cgccggtgtc agttctggcg tacaaggtat cgtttaacca
     5341 gaaataacct gcgtccagac tgtccggttc gagaggaaaa taatcataat tcgacattaa
     5401 tatggataaa ttaccggaaa atggaaaatc attgattatc tcagtataaa tggaagattt
     5461 tatcagacca tcgcggattt tctggcgggt ctcgggatca atagccgcca tctccgttga
     5521 attgacagga ataaaagtca ctggcccgat cttcacctga aatggaacag taatctcata
     5581 aaagccccaa aaggctttgc cgatttgcag gcgggaagta tcgtcggagg ggattatggc
     5641 ttggccgctg accaaaatcg aatcaggcac catattaaaa atcggcgcaa tatcaatctc
     5701 catcattgat tcttgaatta gattagctgg ggatgctttg gcaatcgaac cgtcttccgt
     5761 gatcgtctgg ggtgcgacgc cttttttcaa ccctctcagt actaaactga cattggtacg
     5821 agcttgtatt tcattgaaga aagtcaactt caaaaaaact tggccgaaat ctacattcgc
     5881 taaccccgtc ggaatattgg ctatcgccat tggtggtatg tcaaatgatt cattgaaaaa
     5941 tccttccagc atatctaatt tgagacgcgc aaaatatagg ttaacatcaa tagaggcatc
     6001 acccatatcg gtcgggattg taaccacctg ttcacctgca ttcggtaaaa ttgttgcaat
     6061 tatttcgacc aaaaacgaat ccactaccgt agtaggatca ggattacgaa aaatataacc
     6121 atccaggcgt gcgggattct cgacagttag tccagactga acaatcgtgt caatagtgag
     6181 aggttcattc ctgatataaa aattatgaaa aatcacctgc atcctgagat tgaatggcat
     6241 caggttatga attgtcaaat caagaaaatt ggtatcgagc ggagaggtgg ttgtctgcgc
     6301 aattgtcgcc ttgaaaattg tgttgttaga tggtggcaat aactgatttt gagtagttac
     6361 aatcggcttg ggaattccgg taaagctttc aatttcttca atattcgtgt caaatttgat
     6421 cgcaataaat ggatcggtat tagccggaat tgtcaccggc tgtccctgac tgccagccat
     6481 taccgccgta acaattactt cgattgaatc aaataattgg gcgccgttta aattacctgg
     6541 agtaccgtcg cgtagcgtcg caccggctgg gacattttca taaaaagatt cagcaattag
     6601 cgaaccgttt ttattacgca aggtaattcg gaccgaatcc atagctaccg gcaattggtt
     6661 atcaatttcg gtttggaagg tgccggagcc taccgtcaga taatctaccc gctcaaaata
     6721 aggaacatca tccttgtcaa aaacccgata tttatagcgc tggccatcca attcatagcg
     6781 cgcaatctca cctatgagta aatcattcgg cgaaggtaaa ataccgggaa tagtgataat
     6841 ttccgatagt ttggtagttt gggttaccga tctactaaaa gtcaactgag attcgctgag
     6901 ttccgaaaag ttttgactaa aattaacatt accgacggcg ggaatctgaa aaatattttc
     6961 cgtcagagtt acggtgtcaa gattaccgct aaattgaaaa tagagttggt ctttagtcga
     7021 atcgccgtaa atattattat caggattgac cagatcggct aatggcaaag aaacatcagc
     7081 cagtggcaga tttatcgtct gataataagt cggcatttcc ggcggcgcca gcggctcata
     7141 ggaacagccg caaatcaatc cgatgctaag aaaaaaaata gtcgaataga gcctaaaatg
     7201 ctttaataaa aatagtcctt caataaaaga taaaatccag atcaatttta gtacccagct
     7261 ttatttcttc ttgccaccac gcaccagata ggtagctagc gcaaaatcga aaccttgcat
     7321 cgaatgcacc caaagacctc gattcatacc aagcgcccag tcaatatgca gtgccgcaag
     7381 attcacacta ctaccgatat caaattccca accactaata cctcccagcg ctaatccaac
     7441 ccttaacggc attttgggaa aataggcata ttcaaaaccg accgctgtcc gccacccttt
     7501 cctaagattc agccgatcgc taaacgacat gctaaaatca atcgcagtca gcaaatcttt
     7561 gcgcaattgc tttgccgcgc ccactttcag caccagtggc acttgaaccc gaaaggcact
     7621 tgaatcctgt ttaattaatt tagtatcgct gaaatgatct tgataagtat tttcactgcg
     7681 gaaaagggca tcggcatcta agctgtcaat ccgaaaattg tagaagtaac tctcaccctc
     7741 gaaatcaagt ttcaggtcgg tattttttac tggaaaactt ttgatttgaa atcccatcat
     7801 ttttaaaaca tcaataccat tgtctttgag ccattttgag agcgccgttt cggtattcca
     7861 tttaaccgat tcacccagat tcaaaacgct gaagcccaga gtccagccgt ttaaatcttc
     7921 aaatgcgaca cctaggtctg ccccatagcc gcgaccatga ctggactcgc gaaaataata
     7981 cgagccatta ccgtaaagca caaagtcact cgtgtccacc gcgatttggc cagtactgta
     8041 agcgggatcc aatccataat aacccagacc ttgcaaatac tttaaagaaa tcccaaccga
     8101 cattttgccg aaaggaatcc ctatcgaata aacatactcg acggcagtca tagcatcgca
     8161 tcgcaaatcc aagttatagg tcgttccctt ttcccaattg ccgaaaatga catccagagc
     8221 cggcttggaa agatagtagt ctgagatttg aattaacgag gttgtgaatg ctttattacc
     8281 taccgaaaaa ttcaccaacg gcaaaggcga tgcaacgctg gaattaaggc gataaccgcc
     8341 cttcggtaat ttttctaaaa attcgcgttg tagttcacca ccatcggctg ttaaattttt
     8401 ctcactaaaa cgagccccag attcgagtga ataaaaatta ttggtgacat aaccatttat
     8461 tcctaaaaga taaacgtaag tgcgatattg cgcagtgagc ccgagattgg nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttattccta aaagataaac gtaagtgcga
     8641 tattgcgcag tgagcccgag attggctgga ttctgaccga cacaaaaaat cccgcgcgtt
     8701 atcgcattac cggctccacc cagagcgcgg ttaatcgggc tatcataata ctgagcatcg
     8761 agcggctggg gcaatccgta gagaaaaaac ccaagcagta tcaactgcca caaccaattt
     8821 gatgatctta agattcgggt aagtcgttta ctaagcttct tcggcttcat tttcagtcag
     8881 aatcttttaa attcgccgca acaaaataca agtccagaaa gcccctgaca tgaattgact
     8941 gcgtggcggt aatcgttgcc agactcgtat ccggattaac caacttaaaa taaggtttaa
     9001 agtaacacgt atctgctaac aatccaatcg catcagaact tatctgaaaa atttgacttt
     9061 tgattccggg tactattaaa gtattctcgc taaattgagc gggtggaaat tccaacaccg
     9121 ccaaggtatc aatttctacc acggttgtat cgtccccaag gtggcagata gttccatcga
     9181 aaataacaaa atcgcttggt aaattgcctc caagcgtatc aaatggaaaa aaattaaaat
     9241 tacttattaa aaaataaagg tcaccttgca gttgtgtata attctcgacg gtcatatcca
     9301 gctcaccacc attttgcgac gttattaacc gagcgcgagt tgtgctgtct ttaataaaat
     9361 aggtagttgt attaccggaa gctagtgtaa tctgcggcgg tattttaaac ctcaacggaa
     9421 ccgatatatt atacttgacg ttcacgcccg cattaaaaac agtattgcgc tcaaaatccg
     9481 ccgtggcagt catgactttg acaaaaaccc ggacggtatc aggaagattg gatacaacct
     9541 caccgatatc catctttaaa tccgctcgat ttatcgctat catagtgtca gagaccgttt
     9601 caacaccatg aactttcttt ccactcaaat ggacataaac cgaatcaaaa tctctaccga
     9661 aagtttcaat attcagactc gcccgggcac tgtcaaattc aatatcgctc gctccggtta
     9721 gacttttcgc taccagagcg ggtggcgtct cgcctgtcgc aaacactcgc ccgttaaatg
     9781 attctagagc taattggcca actgtcagcg aagtcgtcag tttttgtttg agactactgg
     9841 tagcctccgc taattcgccc agcacttgga cttcgatatt aaattccaac gaatcaatct
     9901 cttcttttaa taagcctccg atcaacgaag cgccacccag ttcgatcatt atactattct
     9961 gtaaactatt aacaggtaaa tcaagagtag cgactactga ttgacctgga tttttttgaa
    10021 aattcggagc tattacgcga atctgaatcg ggttgggaaa agaatcgctc aactgtgccg
    10081 ccagtaccaa agcgttaatc ggaatattgt cggcagtgaa accggtcgaa ttgtccagaa
    10141 caccagaagt aactcggtta ttagaatcgt cccaggcttt tttcattata taattaatcg
    10201 gattgaccgt gttgttgaca ttacgtttga tattttttct taagtcgagc gtctgactga
    10261 tagtcatagc cggtacaatc cggaaggtcg taggattgat ctcgatcggc atactggttg
    10321 aatcagtaaa cgaataatat agaacaccac tttgatcggc tttaattgga ttgccgttct
    10381 cggtatcaag caaatcctga aatgaatagg atttatctat taagggtaga attattttag
    10441 aattccattt tgcgggaaga ctcggagcag ttggaatttt aaactcacat ttgcccagcc
    10501 agattaggag ggtcagaaaa aggataaacc atttcgaccg gcaatgcgta gtttaagttc
    10561 cttcgctata ggcggtttta tatcgaactt gctctggggt taaagtatca atgttgacac
    10621 ccatagtttg taattttaag agcgcaattt cctgatcctg ctcgacgctg atgtcgtaaa
    10681 ctttctttgc taatttggct ccattttgag ctaggaaaat cattgtcata aattgattag
    10741 caaaggacat atccattact tccgatggat gcccctccgc cgctaccaaa ttcaccaggc
    10801 gtccttcacc cagaacatag atcgaacgac cgttggttaa ttcatgctta acgcaattgg
    10861 gacgcatttc ttgtttcgtt ttggtaaggg tggctaactc tttcaggtta atttcacaat
    10921 cgtagtgacc ggtgttgcag acgatagcgc catctttcat tttttcaaaa tgacgactga
    10981 caatgacatc cttcattccc gtagctgtaa tgaaaatatc gcccagctcg gcggcttgat
    11041 ccatcggcat aactcgaaac ccatccagtt tggctttgag agccgcagtt ggtttaacct
    11101 ccgtgacgat tacattggcg cccatgccac tggcacgttt ggcaacgcct tttccgcaat
    11161 gtccataacc cgcgacaacc attgtcttac cagcataaag tatcgaagtg gcgcgcaaaa
    11221 ttccgtcaat tgaggactgg ccggttccat agacattatc gaaatcccat ttggtttcgg
    11281 catcattgac agccaccatc ggatagagca atttcccatc ctcagccatg gctcgcaaac
    11341 gatgaacacc ggtggtcgtt tcttcggaac cgcccaggac tccagccgcc agttccggaa
    11401 atttattatg aatagtaaaa accagatcgg caccgtcatc aagcgtcaga ttgggttttt
    11461 gaagtaaagt ttgctcgata caccagtaga attcagcggt tgtcaagccg tgccaagccc
    11521 agattggaac accttcttca taaagagcgg cggcgacatc atcttgtgta ctaagcggat
    11581 tgcaaccgct ccaagccact tccgctccag ccgctatcag ggtccgaatc aatacagctg
    11641 tctctttggt gacatgtaag caaccgctga tacgcatccc tttcagcggc ttggaatcgc
    11701 gatatttctg ccttaacgcc atcataaccg gcatatgcga ttccgcccat tctattttca
    11761 actttccaga gggtgccagt gtgatatcct tgatttttcc agtcattact ttactccaaa
    11821 atattgcttt aattcggcga ctttgtcggt tttctcccaa gaaaattctt catcttcgcg
    11881 accgaaatga ccataagcgg ctgttttgag ataaatcgga cgtttgagac acagatattt
    11941 aatgattgcc gccggtttta aggggaaaat cgctcgaacg gcttccgcta aacgttcttc
    12001 aggctgttta ccggtgccgg aagtattaat atagaccgaa atcggatcag caacgccgat
    12061 agaatacgcc agctgaactt cacagcggcg ggctaaacca gccgcaacga tattcttggc
    12121 aatgtaacgt gccatatagg aagccgagcg gtcaactttg gatgggtcct tgccggagaa
    12181 tgctccgcca ccatgccgtc ccataccacc gtaagtgtcg caaattattt tacggccggt
    12241 aacgcccgag tcggcttccg gaccgccgat agcaaaaatg ccagtcgcgt tgatataaaa
    12301 tttagtttca ggagtaagat atccctgaca aactggtaaa atcacatgat ttttaatatc
    12361 cgcgacaatc ttctcatgcg caatttcggg attatgatgt accgccacca ctacattggt
    12421 aattcttttg ggatgatcat cttcatattc gacggtaacc tgggatttac catcaggcct
    12481 gacccagttg agtattcctt ttttccgaac ttctgccaga cgcatcgtca gcttgttcgc
    12541 catactaatc ggtaaaggca ttagctccgg cgtctcgtcg caggcgtagc cgaacatcaa
    12601 accctgatca cctgctcctt gctcgtgttt ctcagtttca ttcacgccga gggcgatatt
    12661 ttcactctgt tcatgaatag atgttatcac agcgcaggaa cgtgcatcca gaccaaagtc
    12721 gggatcggta taaccaattt tttcaatggt ttcgcgtact atccgcggta tatcaacata
    12781 ggctttggtc gtaatttctc cgacgaccat aaccaaacca gtggtcgtca tcgtttcgca
    12841 agcgactcgg ctctccggat caactgtcaa aagtgcatcc agtatagcat ccgaaacctg
    12901 atcacagatt ttatccggat gaccttccgt caccgattct gaggtaaacg aatatttttt
    12961 cattgtctct aactccatta ttaaaattta aaacgttcaa aaatactgta atgtatcgag
    13021 aaaatcaact taatttctga accgtataat cagccgtatc aatctgctga acgattccgg
    13081 agacctgact tttttggcca atcaggctct catctaatat aacgtccttt aatatcgcgc
    13141 cgtccgaaat tatggcattc gataacactg aattcattac ctgaacttca gccccaagat
    13201 gaacattcgg cccaataatc gagcgtttga ttaccgcatc acggccgata taaaccggtg
    13261 gaataatgac tgtctctgga atagcgggtg gcggttgcgg attcttgttg agtaaatagg
    13321 cattggtctt aagcaaagac ttacgcgtac cacaatcgag gttatgttcg atgttaatta
    13381 cctcgaacga ttcgccccat tccagcattt tctgaagggc atcggtcagc tggtattcgc
    13441 ctttagtttt aatattgacg gtaatgatat agtcaatagc ttttttgagc aatgcgcctc
    13501 tttgaattaa atagataccc gcaattgcca gattgctggt agaactagac ggtttctcca
    13561 ccaggcgagt tactttccca tctttcatct ctactattcc aaaccgacgt ggatcatcaa
    13621 ccggcataac gccaatcagg ttttcggagc gactgctcaa ccctttaaaa tcaagttcca
    13681 agataacatc ccctaaaata atcatcacag gtccatcctc aggggttaag cctaatccga
    13741 cggcgtgtcc cagcccacac tgctccaact gctcaatgaa agtaaaatta aagtcgtaat
    13801 tctttaatga gtagtcgcgc acttgctccc cgtaatagcc gattatgact gtcacgtcct
    13861 taattccggt caaggcaagg cggtcataaa tatggccaat tatcggtttg cctgccacag
    13921 gcaggagcac tttcgatcgg gttaaagtca atggcctaag gcgttcgcca atcccggccg
    13981 caggaatgat agctttcaaa atccaacttc ctttctattc taaatctttt ttcagataat
    14041 tctgtaaatc actgatttgt gttttttgcc gaatcgtggc ggcaattttc ttctgaaagt
    14101 taagtgcggc gttggtgccc ataattttca gaacatgatt caaagcaata acctcggcaa
    14161 tcactgtaat atttttaccg ggataaatcg gtagattgat aatcggaata tctacatcga
    14221 gaatcttgtt gtatttttct tgagcaccaa tcctttcgaa gtttttggta ttatcccatt
    14281 cttcgagttc aatctggact tcgattctct tctgctgacg aattgcccgg atgccataaa
    14341 tattccgaac atctacgatt cccagtccgc gtaattctaa atggtgttct aaaatttcta
    14401 ctccactacc gataatgata ttatccgccc ggcgaataat tttgacgaca tcatcagcca
    14461 ccagccggtg gccacgccca accagatcga gagcaatttc actcttaccg atcccgctcg
    14521 gtccggtcag aagcaagccg attccataaa catccacaag actgccgtga attatcgttg
    14581 aaggcgccag ctcgcgatcc agataatcaa ggagagaagc caccaataag gtcgtcgtta
    14641 ccggcgtcgc taacagagcc acctgatatt tacgagcgag cctaacggca caaaccggtg
    14701 gtttaacatc atgagcaaag atcatcagcg gtattttagc tgaaaaaaaa tgctcgaaaa
    14761 tttcttcaac cttttctcct gaaagagttt tgatatagcg aaattcaccc tctccgatca
    14821 actggattcg gtgaggatca aaactttccc agaaaccgga aatttccaat cctggacggt
    14881 tcaatttaga tgaagttacg accgtatcgt gaccaatatc accattgata cgtaaaaatc
    14941 ccaacttctt gcgattttca tgatatatcc gtccgactgt caaatcattc tgattcatcg
    15001 cgcatacctc ttctctttga attttttcaa ttgcctttca atttttgcta tggctaaatc
    15061 aattgatttg atcatatcat cacttgcgac ctgcgcgatc aggtctttgc cgcgcacatg
    15121 aatagagatt tcaatagatt cgccgtcctt agttttctcc ataatgactt tacagctggt
    15181 aatgaggtct tcgtaaagtt taagattgtt catttcctct tcaagatgct ttttggcacg
    15241 atcggtaatt tcataatgac gcgtggttag ttcgatattc atttgaaacc tcctttggct
    15301 atttaaaatt gattatccaa ttcaaaccgc aatcaaaggg aaatgcggca ttggataatt
    15361 tttgggttaa atattttaac ggtaggctat tcatccgact tttatttctt acctgaatcc
    15421 gcgtgcggat gagcttgttg ataaacttct ttcatggtgc taatgcggac atgcgtgtac
    15481 aattgagtcg ttgataaact gctgtgcccc agtaaatctt taactgcatt aagatcagca
    15541 ccatgatcca ataaatgtga agcaaacgag tgccggagaa catgcgggct gagatgagtt
    15601 tgctcggaag cttgttgtaa taaaccgctg attaacttct gcacctgcga tcgcgaaatt
    15661 cgtcggtagc cggtaggttg agaatttggt ccgccgctac tactgataaa cagtggcgag
    15721 tttaattcta acgcgcctac ttcctttttc cgcatattaa gatagtcatt aattacttcg
    15781 gcggcttttt taccaaacgg tactactcgt tctttagcgc ctttgccgaa aacacgcagt
    15841 gtctgccgac tgaaatttaa gtcaccgacg ttgagcgcta tcagttctcc cagccgaatc
    15901 ccgctactat aaaataactc aagtatggcg cgattgcgct tgccgaccaa ggtgttatca
    15961 tccggcagtt cgatagcttt ggcaatttcc tcttcggtta aaacgaccgg cagacgtttc
    16021 ttggctttgg gtgaaagaat caaagccgcc gggttgctat cagcataacc ctgacgggcg
    16081 gcgaatttaa aaaaggcttt gagcgaagcc aatttgcgag agatggaact aatttcgagt
    16141 cctgactctg ataatttacc cagataatga cgaattgcca ttttcgatac ggagttgaga
    16201 tcgattggca cctgattggc atattcagca taaaatagaa aaaattgttc caggtcgcga
    16261 cgataagcca tgatggtatt ttcagaatat ccacgctctt gcgctaggaa tcgcaaaaaa
    16321 tttatcatca tttcttcaac agtaatcaca gctcgattcc tgcttctaat atattcaaaa
    16381 ttctgctaaa atcatcaggt aaaggtgcga ttatctcgat gtctttatca aaaaatgg
//
LOCUS       DAOMJU010000043        16004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_3551_length_16004_cov_20.272870, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000043 DAOMJU010000000
VERSION     DAOMJU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 16004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16004
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3551_length_16004_cov_20.272870"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLO58_05600"
     rRNA            <1..427
                     /locus_tag="PLO58_05600"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            491..607
                     /gene="rrf"
                     /locus_tag="PLO58_05605"
     rRNA            491..607
                     /gene="rrf"
                     /locus_tag="PLO58_05605"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            792..2015
                     /locus_tag="PLO58_05610"
     CDS             792..2015
                     /locus_tag="PLO58_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74300.1"
                     /translation="MKIISKILLFIILLISIVSAQDVNGVLDLRTNYFYNFEVYYLLD
                     FDFNNGRNNPDMFSYSLSYTPADGITPIQIKIEFEWIANIPAVNMYNHRILYVVTRPF
                     EFNGEVTLTTRDIDQNMHNIYYNDGRPCNISVDHFEQDLQRLQSIILSTGGKLPAGSY
                     VFTLNITDQYNTPLKSQTQVIEVSNPTTLELISPGGELADNFEIATTLPTFQWESIDF
                     MWSKTNCPQCGYAIRVAEYDPTRHNSVDQALKDRANLPFPDNNDYFPLPSSKISTVAG
                     LDLYTAGTIFQYPLTGAKPLEAGKIYVWQIRKTFPTTSGPESVLSPIFAFKIQSMGQT
                     SYTGIDVSQYLVLINQIPEINPEWFKEGGLLYGYVPTGTVILNGNQSITLEQLSVLVG
                     QLMTGQVSIKSITIE"
     gene            2027..2716
                     /locus_tag="PLO58_05615"
     CDS             2027..2716
                     /locus_tag="PLO58_05615"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HQO74301.1"
                     /translation="MKKNILMKLLLALILFATSGFSQDKIALILKVRGETSLKRATDS
                     EFKKGVKIGDNLYSRDRIKTGKDGFTALVFLDDKSQIKVRENSEMEITGERSKGSISK
                     QVDMQFGTLKAEINPTKKGDFIIATPTSVASVKGTVFWVLSDPINGDVFYGISGSIEV
                     TNNESGGVIVVGANQTGTSTPTGQVKVEDTPAGAQPVDEDAEESEENNELRIQLRNPS
                     GETKTIKIEYK"
     gene            2744..5356
                     /locus_tag="PLO58_05620"
     CDS             2744..5356
                     /locus_tag="PLO58_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74302.1"
                     /translation="MIELKNKSVRRIVINLSLILTLFSGLAYSQSKILHLPPREVYTD
                     SPVLIEAIIDQHTTEIREVKIYYRESGQEAFIEDYMTEIMGVFKYNIPAQFVTANGIE
                     YLIIAEFVDGSMAAFPEVDPFNVPMFVNARRMTSEPPIALDAVQTDLTGGNSSDVIIL
                     SPEEAQVVAASEVLIAVSLFNAKEIALHTVKIEVDGTNITPIAQIEEDLVLARPNNLP
                     PGNHTVTITLADPNGIYYDPLQWNFRVVGSVREAERVFNYNGRVLAEVNSEQVRGISQ
                     NIQQVTSGFNGSYDWIKFQANVFVTSEESPHKQPRNRYTAGFSSKFFEMNFGDINPQF
                     SEFGLNGKRVRGVEAHLKLNFFNLHVINGETERSIAGIISDTPQMLVSSGDTLYEYSR
                     TGYTYAQRLLAVRPYFGSGKHFQLGFNFIKAIDDTLSVHKEMNGIYTGGNQIIRMEGN
                     TPQDNIVIGSDLKLVFDNQRFVWKNAVALSMLNRNISGGALSKDDTLALGDFNIPIST
                     FGFDPSSIEKMFIINKYLYPISPIALDSVENVQNPIFKIQVDDSTQLAFGINEILNMP
                     SAAFRSTLNLNYFHNYITIKYQHIGSSFYSLGNPYMRKDIQGYLISDRIRLFQNKIFL
                     NINYEQNRDNLNNDKNATTTTSSFLAGISLFLGENLPRIDLNTVQYGRKNDVTNIDTT
                     WYGIPANSFSIKDNRESNITTRQDIRISHNINFLQIKNTLNFSWSSSDRSDRLAGSRP
                     EGYLFSSMKTSMITFSINSNFPFPLQTNLSISNNHSESGLNPEPYDFISIGAQGQYDL
                     FNKALGIYTGYGLTSGKGLADYTQNNIFGGFVFNFRKIHQLRSNVRYVYLDDRSSGEK
                     FHDLCYFVIYSLNF"
     gene            5372..7630
                     /locus_tag="PLO58_05625"
     CDS             5372..7630
                     /locus_tag="PLO58_05625"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQO74303.1"
                     /translation="MKINTLKQAIGGIIIALVLALLVSIFLVFGIFREMELKSLDARF
                     KNRGPKDISHSKLIIVAIDDQTFKSCRERYPYPREYYATLIENLEEVGIKTIMFDIQF
                     TEPDYKNPLGDSILADAIRKNGNVILAGKVTREFSVGGVNVYADKPLPILLETGSDWG
                     VVGEIQDPDGFTRRYPIFEQYQDKTIYTLGTKVFFKETGLPRVPEYKFENGYFIFTDS
                     TTGNTQRLLAHRGSLGWEQTILINYYGPAGTFPTYSMSAIIDDSDFDLKDEEDTDYLE
                     MFKRNSTYPLEMRIALIADSTRQFEAIAALAGGDTASVLAILDEINPFKGKIALVGVS
                     IEELHDNKFTPFYNYGADRRLMPGVETHAHAIQTMLDQSFIRSLPITLNIILIFVISI
                     LTAVAVAFARPVLGGVLALILGFGALVLSYWTFRAHAVWVQLVPLLVATGTSYLSSVV
                     YQFLFEQKEKRKIRSMFQTYMSPKILKYLEDHPDAFSLSGEKREATMFFSDVANFTTI
                     SESLSAEELALVLNKYLSPMTEILMKYDGYVDKYEGDAIMCDFGVPMEDTDHAWKACF
                     AAIDQQAALEVLRPQIKKDHGVDIFVRMGINSGVVSAGNMGSNQRFQYTVMGDAVNQA
                     SRFEGANKQYGTYIMIGEETYHRAQDKIEVRILDRLVVKGKLKPITVYELLGRKGQVD
                     QEMLQICAYFTEGINLYWNRQWDAAIAAFEKAIAVKGEDKPSHVFIERCHIYKNNPPG
                     PNWQGEFVMETK"
     assembly_gap    7726..7825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7974..9542
                     /locus_tag="PLO58_05630"
     CDS             7974..9542
                     /locus_tag="PLO58_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HQO74304.1"
                     /translation="MSPKKGKILWVDDEIELLKAHILFLRSRGFEVVPASNGIDAINL
                     VSQENFDLVLLDEMMSGKDGLETLSDIKARKPGLPVIMITKNEEESLMEKAIGSKITD
                     YLTKPVNPSQVLMACKKILEQQRIASEHVSREYLEEFHVISQRLFNDLATNDWIEIYQ
                     KLARWEIEFDDHPDLGLNETLEDQKRDCNIEFAKFITKNYKKWVNDDPHFRPILSPDL
                     IPQKVLPLLQNGQKVLFLVIDCMRLDQWYTFAPLLYDFYNIDVDYYFSILPSATPFSR
                     NAIFSGLFPSEIQLLYPDIWMPDDEDETSMNRHEAVMLEKLLSEAEIDLRGKFNYMKI
                     LDSDSGWALEKRIASYLGNSLIALVLNFVDILAHRRVDSDFLKEILPNESSYRSVVRT
                     WFQHSWIYSVLRKFADSDFTIILTSDHGSIRVQNDVKVIADKTTSTNIRFKSGKNLNC
                     PPKYALNIKNPAEYKLPDLGINTNYLIAKENFYFVYPTNYHKFQTFFKDTFQHGGISM
                     EEMILPIVTMTPKK"
     gene            9546..10016
                     /gene="tsaE"
                     /locus_tag="PLO58_05635"
     CDS             9546..10016
                     /gene="tsaE"
                     /locus_tag="PLO58_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQO74305.1"
                     /translation="MTLQLVSHSAEDTIRLGERLATYLAPGTVVALIGELAAGKTTLI
                     KGICRGLGVTRSIESPTFTLVNEYPGSIYHIDCYREQRLTEWLELGINEYLYSDGITL
                     IEWADRIQQLLPENTLFINMRQDFNDDTVRYLEIQGSPELISQIANLQFVPNQL"
     gene            10048..10710
                     /gene="tsaB"
                     /locus_tag="PLO58_05640"
     CDS             10048..10710
                     /gene="tsaB"
                     /locus_tag="PLO58_05640"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQO74306.1"
                     /translation="MNSISPRLILGIETATLNCSVGLVHGNQIVGQKNILNRAVHSEK
                     LIELVDEVLKPDFTFADVNAIAVSIGPGSYTGLRIGLATAKGLAFYRQLPILPVPTLT
                     ALEATVRREEIGDAIFFIKSHRDLVYYTVVKKNEPLILKREIAYAPIGMVADLYPQHL
                     LIGDNAFDGQFGERLTVCFPMGEIVARLGADYFEDLLPLSRKAIEPDYHSNLEVKKWP
                     LK"
     gene            10695..11144
                     /gene="rimI"
                     /locus_tag="PLO58_05645"
     CDS             10695..11144
                     /gene="rimI"
                     /locus_tag="PLO58_05645"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HQO74307.1"
                     /translation="MAAKIDARIRRANESDLTAILAIEEKSFTSAWTEDFFRHELYNP
                     VSRFYVLEKEKKIIGYIVIWLLADESHIANIAVHPEFRNAGLGGKLLNFAFEIARRNG
                     AKTITLEVNEQNVAARRLYEKFQFVLTGRRSHYYENKDDALILTRPI"
     gene            11155..12000
                     /gene="accD"
                     /locus_tag="PLO58_05650"
     CDS             11155..12000
                     /gene="accD"
                     /locus_tag="PLO58_05650"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQO74308.1"
                     /translation="MADWFQRTKKGLLGTEKKVIPDGLWVKCPTCNEYLYKRELDKNY
                     WVCTHCSYHFRIRCEEYIKMLVGDKFEEFGLELQSDDPLKFKDSKKYTERLKQAMSQT
                     GSNEAVRTVTGQVDDVPVVLCVMDFNFIGGSMGSVVGEKIARAIDKAREMHLPLIIVS
                     QSGGARMQEGIFSLMQMAKTSAKLTRFHEDGGLFISILTDPTAGGTTASFAMLGDLII
                     SEPGAFIGFAGPRVIKQTIGQELPEGFQHAEFLLEKGFIDIISPRSELHQLVSKILKF
                     FYEPR"
     gene            11987..12769
                     /gene="surE"
                     /locus_tag="PLO58_05655"
     CDS             11987..12769
                     /gene="surE"
                     /locus_tag="PLO58_05655"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQO74309.1"
                     /translation="MSQDKPYILVTNDDGIAAPGINALALALKSIGEVIVIAPDSEKS
                     AVGHAITLSDPIRVTDFSKNGLWRGYSVSGTPADCVKIAVGALLERRPDLVVSGINLG
                     SNAGINVIYSGTVSAATEGTILGIPSIAISLATYSDPIFEPAAEFAIQISQLVLKKGL
                     PERTLLNINVPNVPKEQIRGIRITRQGMANFAELFDRRVDPRNRIYYWMDGKKAELEE
                     GEDVDDVAIRENLISITPVKYDLTNYEFIPELQNWLKEREEK"
     gene            12766..14307
                     /gene="guaA"
                     /locus_tag="PLO58_05660"
     CDS             12766..14307
                     /gene="guaA"
                     /locus_tag="PLO58_05660"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQO74310.1"
                     /translation="MSNISKVWIVDFGSQYTQLIARRVRDMSVYSEIVPSSVTALEAA
                     DQQVSALILSGGPASVNAVEAPQISPDFFEMDIPILGICYGLQLIARHFGARVHSDIH
                     REYGSSRIEIKKQHALFDGISTPTQVWMSHGDHVDSIPDDFEIIATSNNSVPAAMAHT
                     TRRIMGLQFHPEVEHTTEGKKILANFLFDIAGLRPDWTPEKFIEQTIADIRERTGTEQ
                     VLMAMSGGVDSSTMGVLINRAIGRRCIPVFINHGLLRQTEQIQVVRQLKDQMGLPIRS
                     YNYSHQFLSALKGVTDPERKRKIIGREFIRAFEEIARQFPNVKFLAQGTLYPDVIESR
                     SVNGPSQVIKSHHNVGGLPKRMNFQLIEPFSCLFKDEVRKIGRELGIPPEILGRHPFP
                     GPGLAVRILGEVTPRRLSILRRADAIYIEELRSSGYYDKVWQAFAILIPVKTVGVMGD
                     KRTYENALALRAVTSSDGMTADWARLPDTLLSRIANRIVNEVRGINRVVYDVTSKPPA
                     TIEWE"
     gene            14323..>16004
                     /locus_tag="PLO58_05665"
     CDS             14323..>16004
                     /locus_tag="PLO58_05665"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HQO74311.1"
                     /translation="MMKLPRTATIILVISLIWGSSLLADQTTDKLRSLLDEGIACYNA
                     EKYWDAQNIFKIINSIPKDENPYLTTSALMLIKTTYRLGEAEKSIALAYDFLQNYPES
                     KYVKEVKLTLAEVQLSLGNYQESLRIYLDLLKNYPDLEIQSQCEDYLNKIIDFYLEAE
                     TVNALRDSTSDNFSRQYLTIKLAEKYHSEGHTDLAEAELKRLPARLENKYLRDQLQKV
                     EVQLKKPSVVRKYIGVILPLSGSNSASGREILNGVKYAIANYRKNNSEDIVALVFDNR
                     SDGVLSIKQAEFLARSPKVLAIFGPLMTENAVLIAVIANQYQIPMITPTASGSQLASL
                     GPYVFQANVDYENLGRFLGMYGAKVKDIKTVATIAPADEFGKEFTDAFCRAIDEFGGQ
                     VISQQWYQGEPTDLQYQINNLKMTAIEHATNHFEARFNLAKKQLVELVSTDSLYLNDS
                     LRIVVGDKYCFVFKPDTTYRLSIQDALIMTGLMKSTDFEPLDPDTITFPPGIVDGLLI
                     PARANDARLIVPQLIYYDIRIPIFGSANWNEPELLRKNQAVLQNLYFISDYY"
BASE COUNT         4507 a         3275 c         3543 g         4579 t
ORIGIN      
        1 atcgacgggg aggtttggca cctcgatgtc ggctcgtcac atcctggggc tggagaaggt
       61 cccaagggtt gggctgttcg cccattaaag tggcacgtga gctgggttta gaacgtcgtg
      121 agacagttcg gtccttatcc gctgtgggcg caggagattt gcggggggct actcttagta
      181 cgagaggacc agagtggacg aacctctggt gcaccagttg tgccgtcagg tgcatcgctg
      241 ggtagctatg ttcggtcagg ataaacgctg aaagcatata agcgtgaaac cttccccaag
      301 atgatatctc ccttccgttt cggcggaact aaaggcttcc cgtagactac gggattgata
      361 ggtcacaggt gtacgatcag taatgatttt agccgagtga tactaataag ccgtgcgtct
      421 tgaccaaaat ttttatgaaa ttgacttctc taatcgttct cttttcctta acccaattaa
      481 aatccttttt tccggtgata atagcggagg ggaaacaccc gatcccatct cgaactcgga
      541 agttaagccc tccagcgccg atgatactgc cctggcaacg gggtggaaaa gtaggtcatc
      601 accgggaaaa tctttaatcc cgcttcggcg ggatttttat tgtatctcgt ctcccctagt
      661 gttatattta tcgcgtgttc tcaataatta gaagtagttt tagatgaaag tgaagaacta
      721 acccttgttc tatctcacaa tgtcattaat tcaatatctt tagattgaag catgaataaa
      781 atggaggatt tatgaaaata atatctaaaa ttcttctgtt tataatcctg ctgatttcaa
      841 ttgtatccgc tcaggatgtt aatggagtcc tggaccttag aaccaattat ttctataatt
      901 ttgaagtata ttatctactt gattttgatt tcaataatgg ccgcaataat ccggatatgt
      961 tctcctattc gctctcttat actcctgcag atggaattac gccgattcaa attaaaatcg
     1021 aattcgaatg gattgccaac attccggcag taaatatgta taaccatcgg atattatacg
     1081 tagtcacccg accctttgag ttcaatggag aagtaactct gacgactcgg gatattgacc
     1141 aaaatatgca caatatttat tacaatgacg gaagaccatg taatataagt gtagatcatt
     1201 tcgaacagga tttgcaaagg ttgcaatcaa taattctttc aactggtgga aaattgccgg
     1261 ctggaagtta tgtgtttaca ttaaatatta ccgatcagta taataccccc ctaaagagcc
     1321 aaactcaggt gatagaggtt tccaatccga caacgctgga gctgatttcg ccgggaggag
     1381 agttggcaga taatttcgaa attgctacta ccttaccgac ttttcagtgg gaatcaattg
     1441 attttatgtg gagtaaaact aattgcccgc aatgtggcta tgccattaga gtggctgaat
     1501 atgatccaac cagacataat tccgttgatc aggctttgaa agatcgcgcc aacttgccat
     1561 ttcccgataa taatgattat tttccattac catcatcgaa aatctccact gtcgccggat
     1621 tggatttata taccgctggc acgatcttcc agtatccgct caccggggcg aaacccttag
     1681 aagccggtaa aatctacgtc tggcagattc ggaaaacatt tccaaccact tccggacccg
     1741 agtcggtctt gagcccgatt tttgctttta agattcaatc aatggggcaa accagttaca
     1801 ccggaataga tgtatcgcaa tatttagtcc tgataaatca aatacctgag attaatccag
     1861 aatggtttaa agaaggtggc ttactatatg gttatgttcc aactgggacg gtaattttaa
     1921 atggtaatca aagtattacg ctcgaacaat tgtcagtcct ggttggccaa ttaatgaccg
     1981 gtcaagtttc tataaaatct ataacgattg aataggagct ggtaacatga aaaaaaacat
     2041 tttaatgaaa ttattgttgg ctttaatatt atttgctaca agcggattca gtcaagacaa
     2101 gattgcccta attttaaaag ttcgcggcga aaccagcctc aaacgtgcta ccgactccga
     2161 atttaaaaaa ggagtaaaaa tcggcgataa tctctattca cgagacagga tcaaaaccgg
     2221 aaaagacgga tttactgcac tggtttttct cgatgataag agccagataa aagttcgaga
     2281 aaattccgaa atggaaataa ccggtgaacg aagcaaaggt tccatttcaa aacaggttga
     2341 tatgcaattc gggactctga aagcggaaat caatcccacg aaaaagggtg attttatcat
     2401 cgctaccccg acctcggtgg catcggtcaa aggaacagtc ttttgggtac tatctgatcc
     2461 gatcaacggc gatgtctttt atggcatttc aggtagtatt gaagttacca ataatgagag
     2521 tggtggggtg attgttgtgg gtgctaacca gaccggtact tcgactccga cgggccaagt
     2581 caaggtagaa gacaccccag ccggtgccca gcctgttgat gaagatgccg aagaatctga
     2641 agagaataat gaactacgaa ttcaattgcg caatccttct ggtgagacca aaacaattaa
     2701 aatagaatat aaatagataa tatcggatcg agtttttaaa tacatgattg agcttaagaa
     2761 taaatcggta cgccgaattg taattaattt atcgttaatt ttaacgttgt ttagtggatt
     2821 agcctattct caatccaaga ttctgcatct cccgccccgg gaagtgtaca ctgattcacc
     2881 ggttttgatt gaagcgatta tagatcaaca cactacggaa attcgagagg taaaaatcta
     2941 ttaccgagaa tcggggcaag aagcttttat tgaagattat atgactgaaa tcatgggggt
     3001 tttcaaatat aatattccag cgcaattcgt aactgccaat ggcatcgaat atcttataat
     3061 agccgaattt gtagacggca gtatggccgc ttttccggaa gttgatcctt ttaacgtgcc
     3121 gatgttcgtc aatgcccgcc gtatgacgtc cgaacctcca attgctctgg atgcggttca
     3181 aaccgacctt acgggcggaa attccagcga tgtaattatc cttagccctg aagaagctca
     3241 ggttgtagcg gcttcagaag ttctgatagc agtatcttta ttcaatgcca aggagattgc
     3301 tctccatacc gttaaaatcg aagtggatgg aactaatatc accccaattg ctcagataga
     3361 ggaagacctt gttcttgccc gacccaataa tctaccaccc ggtaatcata cagtcacaat
     3421 taccttggcg gatccgaatg ggatttatta cgatccattg caatggaatt tcagggtagt
     3481 tggcagtgta cgtgaagccg aaagggtatt caattataat ggccgcgtgc tggctgaagt
     3541 gaattcggaa caggtgcggg gtatttccca gaatattcag caggtgacga gcggttttaa
     3601 cggttcctat gattggataa aatttcaagc caacgtcttt gtaacttcgg aagagagtcc
     3661 acataaacaa cctcgtaatc gttacacagc cggtttttcc tccaagtttt ttgaaatgaa
     3721 cttcggagat atcaaccccc aattttctga attcggttta aatggaaaaa gagtgcgggg
     3781 agtcgaagcc catttgaaac tcaacttctt caacttacat gttattaatg gagaaaccga
     3841 gcgtagtatc gcaggaatca tcagcgatac accccaaatg ctcgtctcaa gtggtgatac
     3901 tttgtatgaa tactcgcgta ctggttatac ttacgctcag cgcctgctgg cagtgcgacc
     3961 ctacttcggt tccggtaaac attttcagtt aggttttaat tttatcaaag ccattgacga
     4021 tactctttcg gttcataaag agatgaatgg tatttatacg ggtggtaatc agattataag
     4081 gatggaaggc aacacacctc aggacaacat agtcatagga tctgacttga agctggtatt
     4141 tgataatcaa agatttgttt ggaaaaatgc cgtagcgctc tcgatgttaa atcgtaatat
     4201 cagtggcggg gctttaagta aagacgatac gctggcgctc ggtgatttta atatcccgat
     4261 ctcaactttc ggtttcgatc ccagctcaat cgaaaaaatg tttatcatta ataagtatct
     4321 atatccaatt tcaccgatag ctctggattc tgtggaaaat gtgcaaaatc ctatttttaa
     4381 aattcaagtg gatgattcca cccaattagc ctttggaata aatgaaattc ttaatatgcc
     4441 ttccgccgct ttccgaagta cattgaatct taattatttc cataattaca tcactattaa
     4501 ataccagcat attggtagct ctttttattc attgggaaat ccctatatgc gcaaagatat
     4561 tcaaggctat ctaatctccg atagaatccg cctcttccag aacaagatat ttttaaacat
     4621 caattacgag caaaaccgcg ataatttgaa taacgacaag aatgccacaa ctacaacctc
     4681 atcctttttg gccggcattt cattatttct gggcgagaac ttacccagaa ttgatttgaa
     4741 tacagtgcaa tatgggcgga aaaacgatgt taccaacatt gatacgacct ggtatggaat
     4801 acctgcaaac tcatttagca ttaaggataa ccgtgaatca aatatcacga ctcgtcagga
     4861 tattcgcatt tcccataata tcaatttcct ccaaattaag aataccctaa acttctcttg
     4921 gtccagttcc gatcggagtg atcggctggc aggtagccga ccggaaggct atctgttcag
     4981 ttcgatgaaa acttctatga ttacttttag tatcaacagt aattttccat ttcctctgca
     5041 aaccaatctc agcatatcca ataaccacag cgagagcggt ttgaatcccg agccttatga
     5101 tttcatttct atcggagccc aagggcaata tgatcttttt aataaagctc tgggaattta
     5161 tactggctat ggtttgacaa gtggtaaggg attggcagac tacacccaaa ataacatttt
     5221 cggcggattt gtctttaatt tcaggaaaat acaccaactg cgtagtaatg tcagatacgt
     5281 ttaccttgat gaccgaagca gtggcgaaaa attccatgac ctttgctatt ttgtgatcta
     5341 ttccctgaat ttttagatac ttaagcctct aatgaaaata aatacgctca aacaggctat
     5401 aggaggaata attatagccc tcgttctggc gttactggtt tcgatttttt tagtgtttgg
     5461 tatatttcgt gaaatggaat tgaaatcgct ggacgcgcgg tttaaaaatc gcggcccgaa
     5521 ggacatttca cactccaaat taatcattgt agccattgac gatcagactt tcaaaagctg
     5581 ccgtgaacgc tacccttatc cgcgcgagta ttatgctacc ctcattgaaa atctggagga
     5641 agtcggtatt aaaacgatta tgtttgatat tcaatttact gagccggatt ataaaaatcc
     5701 gttgggtgat tcgattctgg cagatgctat tcggaaaaac ggtaatgtca ttctggctgg
     5761 aaaagtcacg cgggaattta gcgtcggcgg cgtcaatgtt tatgccgata agcctttgcc
     5821 gatactgctg gaaaccggta gtgactgggg agttgtcggc gaaattcagg atcccgatgg
     5881 atttacccgc cgttacccga ttttcgaaca atatcaggat aaaacaatct atactctcgg
     5941 taccaaagtt ttttttaaag aaacgggtct gcctcgagta ccggagtata aatttgaaaa
     6001 tggctatttc atttttaccg attcaacgac cggtaatacc cagcgtcttc tggcgcatcg
     6061 cggcagtctt ggttgggaac aaactatcct gatcaattat tatgggccag ccggcacctt
     6121 tccaacctat tctatgtcgg ccataataga tgattccgat tttgatttga aagatgagga
     6181 agataccgat taccttgaga tgtttaagag gaattcgacc tacccactgg aaatgcgcat
     6241 tgcattgatt gcggattcga cccgtcaatt tgaggctatc gcggctctgg cgggcggtga
     6301 taccgccagc gttttggcaa tccttgacga aatcaatccg tttaaaggaa aaatcgcttt
     6361 ggtgggagta tctatcgagg aattgcatga caataaattt acaccgttct ataattacgg
     6421 tgctgatcgg cgtctgatgc cgggagtcga gacgcatgcc catgctattc agactatgct
     6481 ggatcagagt tttattcgtt cccttccgat tacactgaat atcatcttaa tctttgtcat
     6541 tagcatactc accgccgttg cagtggcttt tgccagaccg gtattgggtg gggttttagc
     6601 attaatattg ggtttcggtg cgctcgtatt atcatactgg acgtttcgtg cccacgctgt
     6661 ctgggtgcaa ttggtgccgc tcttggtagc taccggtacc agttatttat cgagtgtcgt
     6721 ttatcaattt ctcttcgaac aaaaagagaa aaggaaaatt cggagtatgt ttcagactta
     6781 tatgagcccg aaaatcctga aatatcttga agatcatccc gacgctttta gcctttccgg
     6841 tgaaaagcgt gaagccacga tgttcttttc ggatgtcgct aattttacta ccatatctga
     6901 aagcctctct gccgaagaac tggctttagt tctaaataag tatttatctc cgatgacaga
     6961 aattctgatg aaatatgacg gttacgtgga taaatatgaa ggtgatgcga ttatgtgtga
     7021 tttcggtgtt ccgatggaag acaccgatca tgcttggaaa gcctgttttg cggctattga
     7081 tcaacaagcg gcgctggaag ttctccgccc tcaaattaag aaagaccacg gcgtggatat
     7141 ttttgtacgg atggggatta attccggagt tgtcagtgcc ggtaatatgg gttccaatca
     7201 gcgctttcag tacacggtta tgggcgacgc ggtcaatcag gcttctcgtt ttgaaggtgc
     7261 taataaacag tatggaacgt atataatgat cggtgaagaa acctatcacc gggcgcagga
     7321 caaaatcgaa gttcgtattc tcgaccgtct cgtcgtaaaa ggcaaattaa agccaattac
     7381 tgtgtatgaa cttctgggcc gcaaaggcca ggttgatcag gaaatgttgc agatttgtgc
     7441 ttattttacg gaggggatta acctctattg gaatcgccag tgggacgccg ccattgccgc
     7501 atttgaaaaa gccattgccg ttaaaggaga agacaaacca tcgcacgttt ttatcgagcg
     7561 ctgtcatatt tataaaaata atccgcccgg accaaactgg caaggcgagt ttgttatgga
     7621 aaccaaatag ccgatgcggt tttccaccta agccagcaga attaagcatt tattttccta
     7681 taaaatatct tttaccaccg accattttgt gtaaattagc tggtgnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnntattt tcctataaaa tatcttttac caccgaccat
     7861 tttgtgtaaa ttagctggtg gtttgtttaa ttttaagttc taaaaattat gaatcctgaa
     7921 acaaaatcgt tccttggaat tattaacaat aaaaagagtg attgagtaat tctatgagtc
     7981 ctaaaaaggg aaaaatatta tgggttgatg atgaaatcga attgttgaaa gcccatattc
     8041 tattcttgcg tagtcgcggt tttgaggtag ttccggcgtc gaatggcatt gatgctatca
     8101 atctagttag ccaggaaaat ttcgatttgg ttctactgga cgaaatgatg tcaggtaaag
     8161 atggactgga gactctatcc gacatcaaag cccgcaaacc cggtctgccg gtaattatga
     8221 ttaccaagaa tgaagaagag agcctgatgg aaaaagcaat cggcagtaag attaccgact
     8281 accttaccaa gccggtcaat cccagccagg tgctaatggc ttgtaaaaaa atactcgagc
     8341 aacagcggat tgcttccgaa cacgtttccc gcgaatatct tgaagaattt catgttattt
     8401 ctcaacgatt atttaatgac ctggcaacca atgattggat cgaaatttat cagaaattag
     8461 ccagatggga aatcgaattc gacgatcatc ctgatttagg cttgaatgaa actctcgagg
     8521 atcaaaaacg cgactgtaat attgagtttg ccaaattcat cactaaaaat tacaagaaat
     8581 gggttaatga cgatccccat ttccgtccga ttctttcgcc ggatttgatt ccgcaaaaag
     8641 tgttgcctct tttgcaaaat ggacagaagg tactgtttct tgttattgat tgtatgcggt
     8701 tggatcagtg gtacactttt gcgccgttat tgtatgattt ttataatatt gatgtggatt
     8761 attacttttc cattctacct tcggctaccc ctttttctcg caatgctatt tttagcgggc
     8821 tttttcccag tgaaattcag ttgctttatc ccgatatctg gatgcctgat gatgaggatg
     8881 agacttcgat gaatcgccac gaagccgtga tgcttgaaaa attgttatca gaagccgaaa
     8941 ttgatctgcg agggaaattt aattatatga aaattctcga ttcggacagt ggctgggctc
     9001 tcgagaaacg cattgcctct tatctaggta attcactcat cgcgctggtg cttaattttg
     9061 ttgacattct ggcacatcgt cgggtagatt ccgatttttt aaaggaaatt ttgccgaacg
     9121 aatccagcta ccgttccgtt gtgcgtactt ggtttcagca ttcttggatt tattcggtat
     9181 tacggaaatt tgctgactcc gattttacca ttattttaac cagcgatcat ggcagtattc
     9241 gcgttcaaaa cgatgttaag gtaattgccg ataaaactac ttctaccaat attcgtttta
     9301 aaagcggcaa gaacttgaat tgtccaccga aatatgccct gaatatcaaa aatcccgccg
     9361 aatataaatt accggatctg ggtattaata ctaattatct catcgctaag gaaaatttct
     9421 attttgtcta tccgactaat tatcacaaat ttcaaacctt ttttaaggac accttccaac
     9481 atggcggcat ctcgatggaa gaaatgatat taccgattgt tactatgacg ccgaagaaat
     9541 agactatgac tttgcaatta gttagtcaca gtgctgagga cactatccgg ttgggcgaaa
     9601 gattggcaac ctatttagct ccgggaacgg tggtcgcctt gattggtgaa ttagccgccg
     9661 gtaagaccac gctaatcaaa ggtatctgtc gtggtttggg agtaacccga tcaatcgaga
     9721 gtccgacttt taccttggtc aatgaatacc ccggctcaat ttaccatatt gattgttatc
     9781 gcgaacagcg tttgacggaa tggctggaac tcggcatcaa tgaatatttg tatagcgatg
     9841 gtataacctt gatcgaatgg gctgaccgaa tccaacaact tctgccagag aatactttgt
     9901 ttattaatat gcgacaggat tttaacgatg acacagttcg ctatctggaa atccagggtt
     9961 cgcctgagct aatctcccaa attgctaatc ttcaattcgt tcctaatcaa ttatgaattc
    10021 ctactttaaa actttgaact ttgaactttg aactctattt ctcccagact gatcctcggt
    10081 attgaaacgg caacgctcaa ttgtagcgtt ggtctcgttc atggcaacca aatcgtaggt
    10141 caaaaaaata ttcttaaccg cgcagtccat tctgagaagc taattgagtt ggttgatgag
    10201 gtactaaagc ctgattttac ctttgcggat gtaaatgcca tcgcagttag catcggaccg
    10261 ggctcgtaca ccggactgag aattggtctg gcgacggcta aagggttagc gttttatcgg
    10321 caactaccga ttttaccggt gccgacttta accgctttgg aagctaccgt gcggcgggaa
    10381 gaaattggag atgcgatctt ttttataaag tcccatcgcg acttggtata ttatactgta
    10441 gttaaaaaaa atgagccgtt aattctgaaa cgagaaatag cctacgctcc gatcggtatg
    10501 gttgccgatc tttatcccca acatttgctg attggcgata acgcatttga cgggcaattt
    10561 ggtgaacgac tgacggtatg ttttccgatg ggggaaatcg ttgcccgact gggtgcagat
    10621 tattttgaag atttattgcc gttaagtcgt aaggctatag agccggatta tcattccaac
    10681 ttggaggtca agaaatggcc gctaaaatag atgcccgaat tcgtcgggct aacgagtcgg
    10741 atttaacggc tatattggca atcgaagaaa aatcgttcac atctgcctgg acggaagatt
    10801 ttttccggca cgaattatac aatcctgtta gtcgttttta tgtactcgaa aaagagaaga
    10861 aaatcatcgg ctatattgtc atctggttgt tagccgatga atcgcacatc gccaatattg
    10921 ccgttcaccc cgaatttaga aatgccggtt taggtggaaa attattaaat ttcgcttttg
    10981 aaattgcgcg tcgtaatgga gcaaagacga taaccctgga agttaatgag caaaatgttg
    11041 ccgctcgtcg tttatatgaa aagtttcagt ttgttttgac cggacgtcgg tcgcactatt
    11101 atgaaaataa ggatgatgct ctgatactga ctcgcccaat ttgatgaggt aaatatggct
    11161 gactggtttc aaagaacaaa aaaaggtcta ctcggaaccg agaaaaaagt tatccctgat
    11221 ggactctggg taaaatgccc gacctgtaat gagtatcttt ataaacgtga actcgataaa
    11281 aattactggg tttgcactca ctgtagctat catttccgga ttcgctgtga agaatatatc
    11341 aaaatgttag tcggagataa gttcgaagaa tttggattgg aactgcagtc ggatgatcct
    11401 ttaaaattca aagattctaa aaaatatacc gagcgtttga aacaggcaat gagtcagacc
    11461 ggctcgaacg aagcggttcg aaccgtaacc ggtcaggttg atgatgtgcc ggtggtatta
    11521 tgcgttatgg attttaactt tatcggcggg agtatgggct cggtcgtggg tgaaaaaatt
    11581 gcgcgcgcta ttgataaagc ccgcgaaatg catctaccat tgataatcgt gtctcaatcc
    11641 ggcggagcgc ggatgcaaga agggattttt tccttaatgc aaatggctaa gactagcgcg
    11701 aaattgacgc gttttcacga agacggtgga ttatttatat ctattttaac cgatccgaca
    11761 gccggtggta cgacggcgag ctttgcgatg ctcggcgatt tgataatttc cgaaccgggt
    11821 gctttcatcg gattcgctgg accacgagtc attaagcaaa ccatcggtca ggaactgcca
    11881 gaaggcttcc aacatgctga atttttactt gaaaagggtt ttattgatat tatctcgcca
    11941 cgttccgaac tccaccaatt agtcagtaaa attttgaagt ttttttatga gccaagataa
    12001 gccctatatt ctggttacta atgacgacgg catcgccgcg ccgggtatta atgcgctggc
    12061 actcgctctg aaatcaatcg gtgaagtaat tgtcattgcg cccgacagtg aaaaaagcgc
    12121 agttggtcat gccattactc tatctgatcc aatccgggtg acggatttta gtaaaaatgg
    12181 attatggcgt ggctattcag tctcggggac accagccgat tgcgtcaaaa ttgcggttgg
    12241 cgcgcttttg gaacgccgac cagacctcgt cgtttccgga atcaatctcg gttccaatgc
    12301 cggcatcaac gtaatttatt ccgggacggt ttcggcggct acggaaggta cgattctggg
    12361 aattccctca attgccatct cactcgccac ttactctgac ccaattttcg aaccagccgc
    12421 agaatttgcg atccaaatct ctcaactcgt tctaaaaaag ggcttaccgg agcggacttt
    12481 attgaacatt aatgtcccta atgtgccgaa ggagcaaatt cgcggcatcc ggattacacg
    12541 ccaggggatg gcaaacttcg ccgaactctt cgaccgaagg gtggatcccc gcaaccggat
    12601 ttactattgg atggatggta aaaaagccga actcgaagaa ggcgaggatg ttgatgacgt
    12661 ggctattcgt gagaatttga tttcgattac gcccgtcaaa tatgatttga ccaactatga
    12721 gtttataccg gaattgcaga attggctaaa agagagagag gaaaaatgag taatattagt
    12781 aaagtttgga ttgtagattt tggttcacaa tatacccaat taatcgcgcg ccgggtgcgg
    12841 gatatgtcgg tttattccga aattgtaccg tcgagtgtga ctgcccttga agccgctgac
    12901 cagcaagtaa gtgctttaat tctatccggt ggtcctgcca gcgttaatgc ggtcgaagcg
    12961 ccacagattt ctcctgattt tttcgagatg gatataccga tattggggat ttgttatggc
    13021 ttgcaactga ttgcgcggca tttcggagcg cgggttcatt ctgatattca tcgagaatac
    13081 ggttcatcta ggattgagat caaaaaacag cacgctctat ttgatggtat ttcgacgccg
    13141 actcaggtct ggatgagtca tggcgatcac gtagattcaa taccagatga ctttgaaatt
    13201 attgcgactt caaataatag tgtacctgcc gcgatggcgc ataccactcg tcggattatg
    13261 ggacttcagt ttcatccgga agtcgaacat actacggaag ggaagaaaat cttagctaat
    13321 ttcctctttg acatcgccgg gcttcgtcca gattggactc ccgaaaaatt tatcgagcaa
    13381 acaattgccg atatccgaga gcgaaccggc acggaacaag ttttaatggc gatgagcggt
    13441 ggagtggatt cctccacgat gggggttctg attaatcgag ccattggccg ccgctgtatt
    13501 ccggttttta tcaatcacgg gctactgcgc cagaccgaac aaattcaagt cgtccggcaa
    13561 ttaaaagatc aaatgggatt gccgattcgt agttataatt attcgcatca atttcttagt
    13621 gccttgaaag gagtaaccga tcccgagcgt aaacgcaaaa taatcgggcg ggagtttatc
    13681 cgggcttttg aagaaattgc ccggcaattt ccgaatgtaa aatttctggc gcaaggaaca
    13741 ctttatccgg atgtcatcga aagtcgctcg gtaaatggtc cctcgcaggt aattaaaagt
    13801 catcacaacg ttggtggtct gccgaaaaga atgaatttcc agctaattga accttttagc
    13861 tgtctcttca aggatgaggt tcgcaaaatc gggcgtgaac tcggaatccc cccagaaatt
    13921 ttaggacggc atccattccc aggtccggga ttggctgtcc gtattctggg cgaagtcact
    13981 ccccgccgac ttagcattct acgtcgagcg gatgccattt acatcgaaga attacgttcc
    14041 agcgggtact atgataaggt ctggcaggct ttcgcaatcc tgattccggt taaaacagtc
    14101 ggcgttatgg gcgacaaacg cacctatgaa aatgccctag ctttgcgtgc ggtcacgagc
    14161 agtgacggca tgactgccga ttgggcgcgc ttaccggata ctttactcag tcgcattgct
    14221 aaccgaatcg tcaatgaagt tcgcggcatc aatcgcgtcg tctatgatgt cacttccaaa
    14281 ccaccagcta cgattgagtg ggaataaatt aagttttaga tgatgatgaa attacccaga
    14341 accgctacca ttattctcgt tatttcgctg atttgggggt cgagcttgtt agccgaccaa
    14401 acgactgaca aactgcgaag tttgctcgat gagggaatag cctgttataa cgccgaaaaa
    14461 tactgggatg cccagaatat ttttaaaatc attaattcca tacccaaaga cgaaaatccc
    14521 tacttgacaa cttccgcttt aatgctgatt aaaacgactt accgcctggg cgaagcagag
    14581 aagagcatcg ctctggctta cgattttctc cagaattatc cggaatcgaa atatgttaag
    14641 gaagtaaaac tgactttagc cgaagtgcaa ttatcgctgg ggaattatca ggaatcactg
    14701 cgaatatatc tggatttatt gaaaaattat cccgatctgg aaatccagtc gcagtgtgaa
    14761 gactatctca ataagataat tgatttttat ctcgaggctg agacagtcaa cgccctgcgt
    14821 gactcgactt ccgataattt ttcgaggcaa tatttaacga taaaattagc cgagaaatat
    14881 cactccgaag gacacactga cttagctgaa gccgagctaa agcgattgcc tgctcgtctc
    14941 gaaaataaat atttgcgcga ccaattacag aaagtcgaag ttcagctgaa gaagccgtcc
    15001 gtcgttcgga aatacattgg agtaatcctg ccattgtcgg gctcaaattc agcttccggt
    15061 cgagaaattt taaatggtgt caaatacgcc attgctaatt atcgcaagaa taattccgag
    15121 gatattgttg ccctcgtatt tgataatcgg agcgatggcg ttctgagtat caagcaagcc
    15181 gaatttttag ctagaagtcc caaagtacta gctatcttcg gcccgctaat gactgaaaat
    15241 gccgttttga ttgcggttat tgccaatcag tatcaaattc cgatgatcac gccgacggct
    15301 tctggtagtc aactggcatc actaggaccc tatgttttcc aggcaaatgt ggattatgaa
    15361 aatctcggac gttttctggg aatgtatgga gcaaaagtaa aggatataaa aactgttgcg
    15421 actatcgcac cggcggatga atttggcaag gagttcaccg acgctttttg ccgtgcaatt
    15481 gatgagttcg ggggacaggt aatttctcag caatggtatc aaggcgagcc gaccgacctt
    15541 cagtaccaga tcaacaattt gaagatgacg gcgattgagc atgctactaa tcatttcgag
    15601 gctcgcttca atctagcgaa aaagcaatta gtggaattag tgagtactga ttctctttat
    15661 ctcaatgatt cactgcgcat agttgtcggc gataaatatt gtttcgtttt taaacccgat
    15721 accacctatc gtctctcgat ccaggatgct ctcataatga ccggcttgat gaaaagcacc
    15781 gattttgaac ctcttgatcc cgatacaatt acttttcccc caggtattgt ggatggatta
    15841 ctgattccag ctcgtgcaaa tgatgctcgg cttattgttc cccaactaat ttattatgat
    15901 attcgcattc ctatttttgg tagtgccaac tggaatgagc cggaattatt aaggaagaat
    15961 caggcggttc tgcaaaatct atattttatt tcggattact atat
//
LOCUS       DAOMJU010000044        15919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_3583_length_15919_cov_22.886031, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000044 DAOMJU010000000
VERSION     DAOMJU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15919
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3583_length_15919_cov_22.886031"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLO58_05670"
     CDS             <1..623
                     /locus_tag="PLO58_05670"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HQO74312.1"
                     /translation="VATAFENIGALKFINPISKILEPVSQFIIDLFPNYPWINIVLAL
                     VILFFALTFLVKVIKSLVLAKVELFFDQYIFKSANRSFLLGLLLTAIVQSSSITTSLT
                     VPLAAAGVLSQKKVFPYAVGANIGTTITAFLAAIATNSVDAITIAFAHTLFNVIGACI
                     FLPLQKIPIELARLLASLTRKNRLYAIAYVILLFFIIPLIIIFITR"
     gene            627..1313
                     /locus_tag="PLO58_05675"
     CDS             627..1313
                     /locus_tag="PLO58_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HQO74313.1"
                     /translation="MFDEFLKIWRKKSLTAETVDQALDMLTKTREMFVESMWSLRESD
                     TGELRLDVYQHDKTINKLVRDIRKKLMTHLMISDKSEVISGLTLAIVVNNIERIGDYT
                     KNIADLAILHKKTLHAAEFEETVVSIEKRVKDFFDWTIKAFPESDVELARQVVHQYKD
                     VSRDTTILINTLIKGECEFNISDAVTLALYIRFLKRVGAHLYNICTSIVNPFHRIGFK
                     EKNNEINQGN"
     gene            1315..2760
                     /gene="proS"
                     /locus_tag="PLO58_05680"
     CDS             1315..2760
                     /gene="proS"
                     /locus_tag="PLO58_05680"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQO74314.1"
                     /translation="MSISVTKRSEDYAKWYTDVVLKAEMADYGPVKGTMVIRPYGYAI
                     WENIQSIADRMFKETGHVNAYFPMFIPESYMKREAEHVEGFAPECAIVTIGGGKPLEE
                     PLYVRPTSETIIWASYKKWIRSYRDLPILINQWANIVRWEMRTRLFLRTTEFLWQEGH
                     TAHATPEEAEEEARRILEIYRQIAEDYLAMPVLVGRKTEAQKFAGAVHTYAIEAMMGD
                     KKALQAGTSHNLGQNFAKAFDVTFLNKSNQLEYVYATSWGISTRLIGGLIMTHGDDKG
                     LILPPRVAPYQVVIVPIWKSEEEKCAINEFVANLTTRLKAEQIRFRYDNSEQETPGWK
                     FNYYEMKGVPIRLEVGPKDVAKNQVVLVRRDPTEMEPIGRKQPIPVDNAPTVIKNLLE
                     EIQTNLFKRAQAFRENNTFQAENYDQFKELIEAGGFIRVFWAGNSADEARIQEETKAT
                     IRVIPFEQKEKGTCFYTGKTTDRIVIFARAY"
     gene            2973..3674
                     /gene="recO"
                     /locus_tag="PLO58_05685"
     CDS             2973..3674
                     /gene="recO"
                     /locus_tag="PLO58_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQO74315.1"
                     /translation="MAIIRTRAFVIKTIPFKDTSLIARLFTESNGKVAVLAKGARNLK
                     SPFRGYLEPLSLLEVQFYYKPTRDIQTLSQAETIRSFLSNSTEIEPTIMATAILECIE
                     KFIREHHEDVPIFKLVLDTLNYIEAQNDNSQLAFVYFILNLTTLMGYEIGLNSSRNSD
                     WSNLPFSMEELEILKKIEKADIRTGLPLCNLNLPIMAIAKTLVNYLAMQTDLPVRLKS
                     FELLSQIIQSADRKD"
     gene            3678..4592
                     /locus_tag="PLO58_05690"
     CDS             3678..4592
                     /locus_tag="PLO58_05690"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQO74316.1"
                     /translation="MNYYNYNQSRYKSGYIRPRFSRGAVGNIIIANVVIYLIMVIFQA
                     QNFFAFNFGLVPYAVLKKGYVWQLFTYMFIHGGFGHIFWNMFVLWMFGMEIEKYWGKR
                     EFYKFYFITGVGSGLITMLFSAHSKIPVVGASGAIYGVLVAFAMLFPERYIYFYFLIP
                     IKAKYFAIIMVVITFFSTLSPGTGNISHLTHLGGLVIGYLYLARYKLLSGWHRRTGSA
                     NWHWPRPKIKNPFRNLHINFQPKKSKKTRDREQAPLSYETEETMREELDRILDKISTE
                     GYDHLSEQERSTLYLLSKYFAEKDRRKS"
     gene            complement(4670..6040)
                     /locus_tag="PLO58_05695"
     CDS             complement(4670..6040)
                     /locus_tag="PLO58_05695"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HQO74317.1"
                     /translation="MKKGRSTLILFGIFVVLFAAVYFFEIKGGEKRAKTSKLASQLFQ
                     IEKDNIKQLILSGDYGEITCEKDGAEWIITAPVLAQGDAQSIESNLDLILGTSIERKI
                     ADSTADLSLFGLLEPRGKVQVVTQSGKHTIVLIGDENPTGDLIFVKYPDNNAIYTTNK
                     SLWTYANKKLYDLRDKKIMHFNLDEVRKINITSRAKGKVSLEKVAGKWQIVDPVNLPA
                     NDSEVKSLLTRLSTGRAKEFIDEEPDDLKKYGLTNPAVMITLEIGESLARTSFLVGDT
                     ATNDGGGFYAKEETRRPVFTVEKWTVDGITKSAFDLQNKKLLGFDNQKCDRIVWQIAN
                     QEYIATKVDSVNWMIVSPETLQVEEDLMRRWLEALRDFSVDELESYQPKSLSVYGLDK
                     PTLRLAFYHKDSKSGELLVGKEISEKYYVKIGNYPHVYRIRKNTFEQIYKKPEDLMIK
                     PVSTES"
     gene            complement(6044..7561)
                     /locus_tag="PLO58_05700"
     CDS             complement(6044..7561)
                     /locus_tag="PLO58_05700"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HQO74318.1"
                     /translation="MKIYKWVFSIGLILIACGLIYFSIVNIWSWPVIVLLILGFGASV
                     FALIKLDIRTLLKNRRLIYGGNMVLVIILVIAIVGLVDFFFTRHSWRVDTTATKIFSL
                     SDQTRKILKNLDKDIEIVVFAKKSNKSYINDRLNEYRHYSKRISWEVVDPDEKPALAK
                     QYNVKNYGNLVIICEDKAELVPNFSEEAIINAIIKVTREGKKKVAFLTGHGEGTIAST
                     ERDGFERAKQAIEQQNYAVEELMLVDKDSIPADISVLVIAGPQKPLFEKELDLLTKFI
                     ENGGSSLIMLDPRPAPGLSDYMKGWKIEVGDNLIIDASGFGRLFGAGPDIPLVTDYTD
                     HPIVKDMKNVMTFFPMARTVRPLESDNKENIIVEELIRTGQQSFAVANIEEVYRTGRV
                     SRGPNDLQGPLSIACAATIENPSGQREGRLVVVGDSDFASNAYFDNQANGDLFMNMIS
                     WLIADEDLIAIRPKNPEISTVNMTPAQTKTVMWLTVIILPAIAFGVAIMVYVRRK"
     gene            complement(7565..8332)
                     /locus_tag="PLO58_05705"
     CDS             complement(7565..8332)
                     /locus_tag="PLO58_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQO74319.1"
                     /translation="MSNLLAIYKRDLKGYFFSPIAYVVIGLFLLVNGIFFYVLLSSFL
                     EYSYAVMIQRQGYPINVNLLMIRPFFQNMSVIVLFVIPMITMRSFSEEKKSGTMELLL
                     TSPVTNWQLILGKFLASFTLYFVMVMVTFLFMVFLLIWGNPSLVPILISYLGILLMGL
                     VLVALGNFISSLTENQIVAAVGTFGATMFLWVIGFAGDFAGKVFGEIFKYLSIVNHFD
                     DFSKGVFDTNHLVYYLSLTFLMLFFTYQSIESSKWRG"
     gene            complement(8334..9281)
                     /locus_tag="PLO58_05710"
     CDS             complement(8334..9281)
                     /locus_tag="PLO58_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQO74320.1"
                     /translation="MIEVQNLVKHYGSVKAVDNISFTVEKGEILGFLGPNAAGKTTTM
                     RIITGYMPASAGTVKVAGYDVFEQGLEVKKRIGYLPENPPLYTDMRVREYLTFVARIK
                     GVNPKTINAEVDRVAERLSVLEVMDNLISNLSKGYKQRVGLAQALLNNPPVLIFDEPT
                     NGLDPRQIIEVREIIKSLGSDHTVILSTHILPEVSMTCNRVAIINEGKLVKIDTPENL
                     TKQLRGSESIILQVEGPMNEVEIALSQHQSVTAVAVQPTGKENIATYRVETSIGSDIR
                     KELASLVVNRGWGLLELKAESLSLEDIFLKLTTKEEEVN"
     gene            complement(9278..10234)
                     /locus_tag="PLO58_05715"
     CDS             complement(9278..10234)
                     /locus_tag="PLO58_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74321.1"
                     /translation="MQLRRSKFNFLALFSFIFILFQLTSAQNSEIIIQTTIDTTIATI
                     GDHLHLRINLRYPIGTIFELPLVEETIGQLEVIDNQLSPPRKKGQINEQEWQLTLAVF
                     DTGQITIPALEIKAHPADDTASVLTFQTEPKTVQVYSVLPPDKRELKDIKPPFPLRRS
                     ISGKAILLILLFLLIVGGIWYYRRWEKAHPPLIIDEKFLEPPHIVTLNQLNALKEKMP
                     TTPDEMRAYYFRISEILREFLERRFFIRALEMTTAEIAEAFKYLDIEPALTREWIELF
                     SELDIVKYAGQTPAEKDIAAVLEKACHCVESTKYETFLRRTM"
     gene            complement(10254..11132)
                     /locus_tag="PLO58_05720"
     CDS             complement(10254..11132)
                     /locus_tag="PLO58_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQO74322.1"
                     /translation="MLSKEILKQVRQIEIRTRHLVNDVFSGEYHSVFKGRGMEFSEVR
                     EYQYGDDFRSIDWNVTARYAHPYVKIQEEERELNVMLAVDASRSNAFGSQGKFKNEIA
                     AEICALLAFSAIKNNDKVGLLIFTDQVEKFIPPHKGRSHTLRVIREILYFQPQSPRTN
                     IKSGLEFLLKGLKRRAIVFLVSDFNDENFAKPLRVLNKKHDVIAIHLQDARDKILPPV
                     GLLNLKDAETGDEVWVDTSDRAFLEQYADLVEQQRQNLRNMARGMKLDLIEIDTAVSY
                     VDPLVKFFKMREQRFR"
     gene            complement(11159..12151)
                     /locus_tag="PLO58_05725"
     CDS             complement(11159..12151)
                     /locus_tag="PLO58_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQO74323.1"
                     /translation="MTFNLAEMNERIQRESAFVSKIKSEMAKVIVGQEQMIERMLIGL
                     LCNGHILLEGVPGLAKSLAVSTLAKIIQVNFRRIQFTPDLLPADLVGTMIYNQKTGTF
                     ETKRGPIFSNIILADEINRSPAKVQSALLEAMQERQVTIGETSHPLDDPFMVLATQNP
                     IEQEGTYPLPEAQVDRFMLKVIVDYPQKDEELDIVKRMARNAAIPAVNPVITAEDIRR
                     ARQTVDDIYIDEKIHKYIVDLVFATRDPKAVNLADIADLIQYGASPRASIFLAMAGKA
                     QAFIAGRGYVTPEDIRTIIWDVMRHRIILTFEAEAEEVTVEEIIRRIIETVAVP"
     gene            complement(12156..12668)
                     /locus_tag="PLO58_05730"
     CDS             complement(12156..12668)
                     /locus_tag="PLO58_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO74324.1"
                     /translation="MRRILFSLLTTGMAVLLITCSKPDNTAFGYWDNAVALFNQSDYQ
                     GCIKQYQRIIKYFPDDSLAVPAMFAMADVYKNNLNDADQAIKIYKEIIRKYGDSGRAA
                     NAHFMIGYVYANEIKDLKKAEEYYKSFLKKYPTNILAPSAEWELKNLGKTLDEIPELQ
                     KITQEKQTAE"
     gene            complement(12779..15391)
                     /gene="clpB"
                     /locus_tag="PLO58_05735"
     CDS             complement(12779..15391)
                     /gene="clpB"
                     /locus_tag="PLO58_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HQO74325.1"
                     /translation="MYSSEKFTIKAQEIVQNAINTAQRLSNQAIEPEHFLYAILTTPD
                     NTGQLVLNKLIGNVEPFTRSVENLLETFPKVSGGSQSYLSNASNKMLQVAEDQAKQLQ
                     DEYLSAEHLLLGIIEVAKGELKNLLKKNGISNETILSALKEIRGSQTITDQFPEDKYQ
                     ALKKYCRDLNELARRGKLDPVIGREEEIRRVLQVLARRTKNNPVLIGEPGVGKTAIAE
                     GLALRIVSQDVPESFRDKRILALDMGALIAGAKFRGEFEDRLKAVLREISEAQGKIIL
                     FIDEIHTIVGAGAAEGAVDASNLLKPALARGDLRCIAATTFSEYRKYIEKDKALERRF
                     QPVLIDEPSIEDSISILRGLKERYENHHGIRITDAALVAAVELSARYITDRFLPDKAI
                     DLIDEAASRLRLEIDSLPAELDEVERKISQLEIESRALAKEKDIEESRQRYEKIQQEL
                     AQLKDEAAVLRARWQNEKKHITAISELKKQIEAKKLEAEKAEREGNWERAAQLKHYEL
                     VELNKQLEAEKEALKNVSGKRALLREEVTEEDIAEIVSKWTHIPVSRLVEGEREKLLK
                     MEEKLHERVIGQDEAIKAVSNAVRRARAGLQDENRPLASFIFTGSTGVGKTELAKALA
                     EFLFNDENALIRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILLD
                     EIEKAHSDVFNILLQVLEDGRLTDNKGNVANFKNSIIIMTSNLGSDLIMRQAGDVDEN
                     NIDVIYANIQETVMSQLRQILKPEFLNRIDEIIVFKPLLPKEIREVVRLQFNRLIERV
                     KTRGIQVKLSDEACDLIAKAGWDPAFGARPIKRIIQKQILDPLALEILEEKIQPGDTV
                     NIEIRDGKIHFVKG"
     gene            complement(15404..>15919)
                     /locus_tag="PLO58_05740"
     CDS             complement(15404..>15919)
                     /locus_tag="PLO58_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQO74326.1"
                     /translation="LSGKIALVGGKPADYYKLEPSFSQYFNILGPVVLAYRVKVGIMA
                     PLRTGQPTPEYEKFYLGGSTSLRAWPDNKFEPDHIDPDGYNFKGLTSVEVRFPLFWLF
                     GGEVFVDGGNLAGEPDFSEFFKTYRWNYGFGLTLATPLGPIRIDYAIKIPKENEKKQP
                     GRFQLGIPYAF"
BASE COUNT         4448 a         3578 c         3317 g         4576 t
ORIGIN      
        1 ctgtggcaac tgcttttgaa aatatcggcg ctttgaagtt tatcaatcca attagtaaaa
       61 ttttggaacc ggtatcgcaa tttattattg atttatttcc caattatccc tggataaata
      121 tagttttggc tttagttatt ctgttctttg ctctgacatt tttagtcaaa gtgattaaat
      181 cgctcgttct cgctaaagtt gagctatttt ttgatcagta tatttttaaa agcgctaatc
      241 gcagctttct actgggcttg ttgctgaccg cgatagttca aagtagttcc attaccacca
      301 gtttaacggt cccgttggcg gccgccggag tattgtcgca aaagaaggtt tttccctatg
      361 ctgttggggc aaatatcggt acgacaatca cggcttttct tgccgctata gccactaatt
      421 cggttgatgc tattacgatt gcctttgctc atactttatt caatgtaatc ggcgcatgta
      481 ttttcctacc cctccaaaaa atccccattg agctagcccg gttattagcc agtttaaccc
      541 gtaaaaaccg gttatatgcc attgcctatg ttatcttgct tttctttatc ataccattaa
      601 ttattatatt tataacgagg taagatatgt ttgatgaatt tttaaaaatt tggagaaaaa
      661 agagcttgac tgcggaaact gtggatcaag cccttgatat gctcactaag acccgcgaga
      721 tgtttgtaga atcaatgtgg tctctacgcg aaagtgacac cggggaatta cgcctcgacg
      781 tttaccaaca tgataaaacc attaataaat tggtgcggga tattcgtaaa aaattgatga
      841 cccatctgat gatttccgat aaaagcgagg tcatttccgg gttaacgctt gccattgtcg
      901 tcaataatat tgagcgtatt ggtgattata ccaaaaacat cgctgatttg gcaattctgc
      961 ataaaaagac tctccatgcc gccgagtttg aggagactgt agtcagtatc gaaaaacggg
     1021 taaaggactt ttttgactgg acaatcaaag cctttccgga atcggatgtt gagttggcgc
     1081 gccaggtcgt ccatcagtat aaagatgttt cccgcgatac gactatttta atcaataccc
     1141 ttatcaaagg cgaatgcgaa ttcaatattt ccgatgcggt gactctggcg ctatacattc
     1201 gttttttgaa acgcgtcggg gcgcacctct ataatatttg caccagtatt gtcaatccct
     1261 tccatcggat cggtttcaaa gaaaaaaaca acgaaataaa tcaaggaaat taacgtgtca
     1321 attagtgtta caaagcgttc cgaagattac gccaaatggt atactgatgt cgttttgaaa
     1381 gccgaaatgg cggactatgg tccggtcaaa ggcacgatgg ttattcgccc ctacggttat
     1441 gcgatctggg aaaatattca gagcatcgct gatcggatgt ttaaagaaac tggtcacgtc
     1501 aacgcctatt ttccaatgtt cattccggaa agttatatga agcgcgaagc cgaacatgtc
     1561 gaaggctttg cgccggaatg cgccattgta acgatcggtg gcggcaaacc tcttgaagaa
     1621 ccgctgtatg tgcgcccaac ttccgaaacg atcatctggg caagttataa aaaatggatt
     1681 cgcagttatc gcgatttacc gatattgatc aatcaatggg cgaatatcgt ccgctgggaa
     1741 atgcgtaccc gccttttttt gcgaacgacc gaatttctct ggcaggaagg tcacaccgcc
     1801 cacgccacgc cggaagaagc cgaagaagaa gcccgccgca ttctcgaaat ttaccgccag
     1861 attgccgaag attatctcgc catgccggtt ttggtcggtc gtaaaaccga agcgcagaaa
     1921 tttgccggag cggttcatac ttacgctatt gaagcgatga tgggcgataa aaaagctctg
     1981 caggctggga cttcgcataa tctcggccag aattttgcca aagctttcga cgtcactttt
     2041 ttaaataaat ccaaccagct cgaatatgtc tatgctacca gctggggcat cagcacacgc
     2101 ttgatcggcg gattgattat gacgcatggc gatgacaagg gattaatttt gccgccgcgc
     2161 gttgcgccgt atcaagtcgt aattgtgcca atctggaaaa gcgaagaaga aaaatgcgca
     2221 atcaatgaat ttgtcgctaa tctcaccacc agattgaaag ccgaacaaat tcggtttcgt
     2281 tatgataact ccgaacagga aactcccggc tggaaattca attattatga aatgaaaggg
     2341 gtcccgattc gtctggaagt cggtcccaaa gacgtcgcta aaaaccaggt cgttcttgtt
     2401 cgtcgtgatc cgaccgagat ggaacccatc ggacgtaaac aacctattcc ggttgataat
     2461 gcaccgacgg ttatcaaaaa cttattggaa gaaattcaaa ctaacctctt caaacgcgcc
     2521 caagccttcc gcgaaaataa cacctttcag gctgagaatt atgaccaatt caaagagtta
     2581 atcgaggcgg gtggattcat ccgggttttt tgggcgggca atagcgctga tgaagctcgc
     2641 attcaagaag agaccaaagc cactattcgg gtcattccat ttgagcaaaa agaaaaaggc
     2701 acttgttttt ataccggcaa aaccactgac cggatcgtca tctttgctcg agcttattaa
     2761 aatttaacga cggcaatcaa ttagcagctc agtcgtcatt ggtagtattt cgggagtcag
     2821 gaaattgtca ttaatcgtgt ttcgttgtca ttcattgtga tttattatga tttattgtga
     2881 ctaatacaga aagtccaaaa ttgaatactt ttattttcaa taagaaaatg tgatcgagat
     2941 ggcttgattt aggattaatc cagaggaaaa taatggcaat aattagaacg cgcgccttcg
     3001 taatcaaaac tattccattt aaagatacta gcttgattgc acgtctgttt accgaatcaa
     3061 acggtaaagt tgcggttctt gccaagggcg ctcgcaacct gaaaagccct ttccgcgggt
     3121 acttggagcc actaagttta ctggaagtcc aattttatta caagccgact cgcgatattc
     3181 agacgctcag ccaagccgaa actatccgga gttttttgtc caacagtact gaaattgagc
     3241 cgaccattat ggcaaccgcc attttggaat gtattgaaaa gttcattcgc gagcaccatg
     3301 aggatgtacc gatttttaaa ttggttttgg acactttgaa ttatatcgag gcgcagaacg
     3361 ataatagcca acttgctttt gtttatttta tattgaattt gacgacgctg atgggatacg
     3421 agatcggtct caattccagt agaaattcgg actggtcaaa cttaccattt tctatggagg
     3481 aacttgagat tctgaaaaaa attgaaaaag ctgatatccg caccggtttg ccgctctgca
     3541 acctaaattt gccaattatg gcgattgcca aaactttggt gaattattta gcaatgcaaa
     3601 ccgatttacc cgtcagattg aaaagcttcg aacttctctc gcaaattatc cagtcagccg
     3661 atagaaagga ttagccaatg aattattaca attacaacca atcgcgttat aaatccggtt
     3721 atattcgccc gcgtttttcg aggggcgccg tgggcaatat cattatcgct aatgttgtga
     3781 tttatttgat tatggtgatt tttcaggctc agaatttttt tgcctttaat tttggattgg
     3841 taccctacgc cgtcctgaaa aagggctatg tttggcaatt atttacttac atgtttattc
     3901 atggtggatt cggccacatt ttctggaata tgtttgtgtt gtggatgttc ggcatggaaa
     3961 tcgagaaata ttggggtaaa cgcgagtttt acaaatttta cttcataacg ggagttggtt
     4021 cgggcttaat aacgatgcta ttcagcgctc attctaaaat tccggtggtc ggtgccagcg
     4081 gggcaattta tggtgtgctc gtggctttcg cgatgctctt tccggaacga tatatttact
     4141 tttacttcct gattccgatt aaagccaaat attttgctat cattatggtc gtaattactt
     4201 tcttttcgac cttgtccccg gggacaggca atatcagtca tctaacccat ctcggcggac
     4261 tggtaattgg ttatctttac ttagctcgtt acaaattact ctccggttgg catcgtcgga
     4321 cgggttcggc aaactggcat tggccacgcc ccaaaatcaa aaatcccttc cgcaatctgc
     4381 atattaattt ccagccgaaa aaatcaaaaa agaccagaga cagagaacaa gcacctctct
     4441 cttatgaaac cgaagaaact atgcgtgagg aacttgatcg cattttagat aaaatcagca
     4501 cggaaggcta cgatcacctt tccgagcagg agcgctcgac tttataccta ctaagtaaat
     4561 attttgccga aaaagaccga cgtaaaagtt aaatggttac tctaaaaaat gcctatgaat
     4621 tttcctaaag tagtcccgca aaggaaggaa attcgtagat taatttatat taactttccg
     4681 tagaaacggg tttaatcatc aaatcttctg gctttttata aatctgttcg aaggtgtttt
     4741 tcctgattcg ataaacgtgc ggataattac caatttttac ataatacttt tcggaaattt
     4801 ccttgccgac taaaagttca cccgatttgg aatctttgtg gtaaaaagcc agtcgcaaag
     4861 tgggcttatc cagaccatac acagatagcg atttaggttg ataagattcc agctcgtcaa
     4921 cgctaaaatc ccgcaaagcc tccagccagc ggcgcatcaa gtcttcttca acctgtagag
     4981 tttccggcga tacaatcatc caattgactg aatcaacttt cgtagcaatg tattcctgat
     5041 tagcaatctg ccagacgatc cgatcacatt tctggttatc gaatccaagt aactttttat
     5101 tctgcaaatc gaaagcactc ttagttatac catccacagt ccatttttca acagtaaaga
     5161 ctggtcgccg ggtttcttct ttagcgtaaa aaccgccacc atcattggtt gcagtatcac
     5221 caactaaaaa actggtacgc gccaggcttt cgccaatttc aagtgtgatc ataacagcag
     5281 gattggtcaa cccatatttt ttaagatcat ccggttcttc gtcaataaac tccttcgctc
     5341 taccggttga taggcgagtg agtaacgatt tgacttcgct atcatttgcc ggtagattta
     5401 ctgggtcaac gatttgccat tttcccgcga ctttttccag actgactttt ccttttgccc
     5461 gggaagtgat attgattttg cgcacttcat ctaaattgaa atgcattatc tttttatcgc
     5521 gcaagtcgta tagtttttta ttagcataag tccagagtga tttattggta gtataaatcg
     5581 cattgttatc cggatacttg acaaaaatca ggtcgccggt tggattctcg tcaccgatca
     5641 atactatggt atgtttacca ctttgagtaa cgacctgcac ctttccgcgc ggttctaata
     5701 aaccaaacaa acttaaatcg gcggttgagt cggcaatctt tcgttcgata ctagtcccta
     5761 ggattaaatc gaggttggat tcgatactct gggcgtcgcc ctgggctaac accggtgcgg
     5821 tgataatcca ctccgctccg tctttctcgc aagtaatttc tccataatct ccggagagaa
     5881 tcaattgttt gatgttgtct ttttcgatct ggaagagttg gctagctaat ttgcttgtct
     5941 ttgcccgctt ctcgccgcct ttaatttcaa aaaaataaac agccgcaaac aagacaacaa
     6001 aaatcccgaa taaaatcaga gtcgatcggc cttttttcat ggtttatttc ctcctgacat
     6061 aaaccataat cgccaccccg aaagcaattg ctggcaaaat tataacggtc agccacataa
     6121 ccgttttggt ctgggcggga gtcatattaa ccgtgctaat ttccggattt ttaggacgaa
     6181 tcgcgatcag gtcttcgtcg gcaataagcc agctgatcat attcataaat aaatcgccat
     6241 tagcttgatt atcgaaatag gcattcgaag caaaatcgga atcgccgaca actaccagcc
     6301 gaccttcacg ctgaccggaa gggttctcaa ttgtagccgc acaggcgatt gaaagtggac
     6361 cctgcagatc attcggacct ctggaaacgc gaccagtgcg gtaaacttcc tcgatattgg
     6421 caaccgcaaa actctgctga ccggttcgga ttaattcctc gactattata ttctctttgt
     6481 tatcagactc cagcggtcta actgttctag ccattgggaa gaaggtcatt acatttttca
     6541 tatcctttac aattggatga tcggtatagt cagtaacaag cggaatatct ggcccggcac
     6601 cgaaaaggcg gccaaatcca ctggcatcaa taattaaatt atcgccaacc tcaatcttcc
     6661 accctttcat ataatcgctc aaaccgggtg cgggacgcgg atccaacatt atcaaactac
     6721 taccaccgtt ttcgatgaat tttgtcagca gatcaagttc tttttcgaac agcggttttt
     6781 ggggaccggc aattaccaaa actgagatat ccgccggaat cgaatcctta tcaaccaaca
     6841 tcagttcctc gacggcataa ttttgctgtt cgatagcctg tttggctcgt tcaaagccat
     6901 cgcgttcggt gctggcgata gtgccctcgc cgtgcccggt caagaaggca actttcttct
     6961 taccttcgcg agttacctta ataatggcat taataattgc ttcttcagaa aaattgggaa
     7021 ccagttccgc tttatcttca caaataatca caagattacc gtaattctta acattatatt
     7081 gcttagcaag ggctggtttt tcatccggat caacgacttc ccaactaatg cgtttggaat
     7141 aatggcgata ttcattgaga cgatcattga tgtaagactt attggatttc ttggcaaaaa
     7201 cgacaatctc aatgtcttta tccaggtttt tcagaatctt acgagtctga tcggaaagac
     7261 taaagatctt ggtggcggtg gtatctaccc gccatgaatg acgggtaaag aaaaaatcaa
     7321 ctaatcccac aatagcaata actagaataa taaccagtac catattgcca ccgtagatca
     7381 gcctccgatt tttcagcaaa gttcggatat ccaatttaat caaagcgaac acgctggcac
     7441 caaagccgag aatcaataaa acaatcaccg gccaagacca gatattgacg atagaaaagt
     7501 aaattaaccc gcaggcaatt aaaattaatc caatcgaaaa aacccattta taaattttca
     7561 tgacttaacc cctccacttg gaagattcaa ttgactgata agtaaaaaac aacattagaa
     7621 aagtcaacga gaggtaataa accagatgat tagtgtcaaa gactcctttg gagaaatcat
     7681 caaaatgatt gactattgat aagtatttga atatttcacc aaacacttta ccagcaaaat
     7741 ctccagcgaa accgattacc caaaggaaca ttgtcgcgcc gaaagttccg accgcggcga
     7801 cgatctggtt ttcggtcagc gaggaaataa aattgccgag agcaacaaga actaagccca
     7861 tcagcagaat tccgaggtaa ctaatcaaaa tcggtacgag gctgggattg ccccagatta
     7921 acagaaaaac cataaaaaga aatgttacca taaccattac gaaataaagg gtaaaagatg
     7981 ccagaaattt tccgagaatc aactgccaat tggtcacagg cgaagtcaac aagagttcca
     8041 tagtcccgct ttttttctcc tccgaaaaac tgcgcatagt gatcatcgga atgacaaaca
     8101 acacgatgac gctcatattt tggaaaaacg gtcgaatcat aagaagatta acattaatcg
     8161 gataaccttg tctctggatc attaccgcat agctgtattc caggaaacta gaaagcaata
     8221 cgtagaaaaa aataccattg actaaaagga acaatccgat tacaacataa gcaattggag
     8281 agaaaaaata tcctttcagg tcgcgtttat aaattgccag taaattactc atttcaattg
     8341 acctcctctt ctttggtggt gagtttcagg aagatatcct cgaggctcag gctttccgct
     8401 ttcagttcga gcaaacccca accacgattg accactagag atgccagctc tttacgaata
     8461 tcagaaccga ttgaagtctc caccctataa gtagctatat tttccttgcc cgttggttga
     8521 actgccacgg cggtcaccga ttgatgctga ctaagggcta tttcgacttc gttcattggt
     8581 ccctcgacct gcagaataat agattccgag ccgcgtaatt gcttcgtcaa gttctcaggt
     8641 gtatcaatct tgaccaactt accttcattg ataatggcga ctcgattaca ggtcatactg
     8701 acttctggca aaatatgagt cgaaaggatg acagtatgat cactaccgag actcttgatt
     8761 atttcacgta cctcaattat ttgacgcgga tcgagaccat tggttggttc atcgaaaatt
     8821 agaaccggcg gattattcag cagagcttgc gcaagaccga ctcgctgttt gtaacccttg
     8881 gagagattgc taataagatt atccataact tctaaaaccg acagacgttc ggcgacgcga
     8941 tcaacttcgg cgttgatagt tttgggattg acacccttga tgcgagcgac gaaagtcaaa
     9001 tattcacgca cgcgcatatc ggtataaagc ggcggattct ctggcaaata gccaatgcgc
     9061 tttttaacct ccagaccttg ttcaaagacg tcgtacccgg ccactttgac cgttccagct
     9121 gaagctggca tataacccgt gataatgcgc atcgtggtcg ttttgccagc ggcatttggt
     9181 ccgaggaagc cgaggatttc gcctttttca acggtgaaag aaatattatc cacggctttg
     9241 accgaaccgt aatgttttac caaattttgt acttctatca catcgtcctc cgtaaaaatg
     9301 tttcatattt ggtgctttca acacaatggc aggctttttc caatactgcc gctatatctt
     9361 tttcagccgg ggtttgaccg gcatatttga caatatcgag ttccgagaaa agctctatcc
     9421 actccctggt cagcgcgggc tcgatgtcca gatatttaaa agcctcagca atttccgccg
     9481 tggtcatttc cagtgctcgg ataaaaaagc gtcgttctaa aaattcccgc agaatctccg
     9541 aaatgcgaaa gtagtaggct cgcatttcgt cgggcgtggt gggcattttc tctttcaaag
     9601 cattcagctg attcaaagta actatgtgcg gtggctccag aaatttttca tcaataatta
     9661 gcggtggatg ggctttctcc cagcgtctat aataccaaat tccgccgaca atcagcagga
     9721 acagaagtat taacaaaata gctttccctg agatactccg acgtaaaggg aaaggcggtt
     9781 taatgtcttt cagttcacgc ttatctggcg gtagaaccga atagacctgc acggttttcg
     9841 gctcagtttg aaaagtcagc acgctggcag tgtcgtccgc cgggtgagct ttgatttcca
     9901 gtgccggaat tgtgatttgt ccagtatcaa aaacggccag agtcaactgc cactcctgct
     9961 cgttaatttg ccccttcttg cgtggtggcg aaagttggtt atcaattact tctaactggc
    10021 cgatcgtttc ctcgactaac ggcaactcaa aaatcgtccc gatcggatag cgcaggttga
    10081 ttcgcaaatg gaggtgatcg ccgatcgttg caatggtggt gtcaatagtg gtttggataa
    10141 taatctcgga gttctgggct gaggttaatt gaaataaaat aaaaataaaa ctaaagaggg
    10201 cgagaaagtt aaattttgag cgacgtaact gcattgaaaa ccttaagttc aaatcacctg
    10261 aacctttgct cccgcatctt aaagaactta accagagggt caacgtaaga aacagcagtg
    10321 tcaatttcga ttaaatccag cttcataccc cgcgccatat tacgcaaatt ttgacgctgt
    10381 tgctcgacta aatcggcata ctgttcgagg aatgcgcggt cggaggtgtc cacccagact
    10441 tcatctccgg tttcagcgtc tttcaggttt aataagccta ccggcggtaa tattttatca
    10501 cgagcatcct ggagatgaat tgcgataaca tcgtgttttt tattcaatac ccgtaacggt
    10561 ttggcgaaat tttcgtcatt gaaatcagaa accagaaaga cgatcgcgcg ccgttttaag
    10621 cctttcagca gaaactccag cccggattta atattggtgc gcggcgattg tggctgaaag
    10681 tagagtattt cccgaatcac gcgtaaagta tgtgaccggc ctttatgagg tggaataaat
    10741 ttttcaacct gatcggtgaa aatcagtagg ccgactttat cgttattttt tatggcggag
    10801 aaagccagta gcgcacaaat ttcagccgct atttcattct tgaatttacc ctgcgaaccg
    10861 aaagcatttg agcgcgaggc atctaccgcc agcataacgt tcagttcacg ctcctcttct
    10921 tgaattttca catagggatg agcataacgg gcagtcacat tccaatcaat ggaacgaaaa
    10981 tcatcgccat actgatattc acgtacttcc gagaattcca taccgcgtcc tttaaaaacc
    11041 gaatgatact caccactaaa gacatcattg acgagatgcc gggtgcgtat ttcaatctga
    11101 cgaacctgtt tgagaatttc cttagacagc ataacgagat aatattttgt ggaaaaaatt
    11161 acggtaccgc gaccgtttcg atgatgcgcc tgataatttc ttcgactgtc acttcttcgg
    11221 cttccgcctc gaaagtcagg atgatgcggt ggcgcataac atcccagata atcgtacgaa
    11281 tgtcttccgg tgtcacataa ccgcggcctg cgataaaagc ctgcgcttta ccagccatcg
    11341 ccagaaatat tgaagcacgt ggcgaggcac catactggat caaatcggcg atgtcggcca
    11401 ggttaacagc ctttgggtcg cgggtggcaa aaaccagatc gacaatatat ttatgaattt
    11461 tttcatcaat gtaaatatca tcaactgttt ggcgagcacg tcgaatatcc tccgcggtta
    11521 taaccggatt gaccgctgga atagcggcgt tgcgcgccat acgtttgaca atgtcgagtt
    11581 cttcatcttt ctgcggataa tcaacaatca ccttgagcat aaaacggtca acctgagcct
    11641 cgggcagagg ataagtccct tcttgctcaa tcggattttg cgtcgccaaa accataaacg
    11701 ggtcgtccaa cggatgactg gtttcgccga tcgttacctg acgttcttgc atagcttcca
    11761 gcaaagcaga ctgcaccttc gctggcgagc ggttgatttc atcagccaat ataatattgc
    11821 taaaaatcgg accgcgttta gtctcaaaag ttccggtttt ttgattataa atcatcgttc
    11881 cgaccaaatc agccggcaaa aggtccggtg tgaactggat gcggcgaaaa ttgacctgga
    11941 taattttagc caatgtgcta acagctaatg acttggcaag gccaggcacg ccttctaata
    12001 gaatgtggcc attgcagagc aaaccaatca gcatacgttc gatcatttgt tcctgcccga
    12061 cgattacctt agccatttca ctctttattt tagaaacaaa agcactttcg cgctgaatgc
    12121 gttcattcat ttcagctaaa ttgaaggtca tagctttact ccgcagtctg tttttcttgg
    12181 gttatttttt gtaattcggg aatttcatcg agggttttgc cgaggttttt cagttcccat
    12241 tcagctgagg gcgccagaat attggtggga tactttttta ggaaggattt ataatattct
    12301 tcggctttct ttaagtcctt aatttcattg gcgtaaacat atccaatcat aaaatgagca
    12361 ttagccgctc gtccggaatc accgtactta cggatgatct ctttataaat tttaatcgct
    12421 tggtcggcat cattcagatt atttttatat acatcagcca ttgcgaacat agccggcacc
    12481 gccagactgt catcgggaaa atatttgatg attctttggt actgtttgat acatccctga
    12541 taatctgact ggttaaagag agccacagca ttatcccaat atccgaaagc agtgttgtcc
    12601 ggtttactac aggtaataag aagcacagcc attcctgttg tcaataacga aaacaatatt
    12661 cggcgcataa tccaaagcct cctaaataaa tcagttaata tctaatattg atgcacaagc
    12721 attttcgcac gataattcgg ctttaacgga atggcaattc gaaatgttcc tctatcgttt
    12781 agcctttgac gaaatggatt ttgccatcac gaatttctat attgaccgta tcgccgggct
    12841 ggattttttc ttccagaatt tcaagggcga gcggatcaag aatctgtttc tggataatgc
    12901 gtttaatagg ccgtgccccg aaagccggat cccagccggc tttggcaatt aaatcacaag
    12961 cctcgtctga taatttgacc tgaattccac gcgttttgac acgctctatc aagcgattga
    13021 actgcaatct taccacttcg cgaatttctt taggtaaaag cggtttaaag acgatgattt
    13081 catcaatccg gtttaaaaat tctggcttaa gaatttgtcg cagttgtgac ataacagttt
    13141 cctggatatt ggcataaatg acatcaatgt tgttctcatc aacgtcacca gcctgtctca
    13201 taatcaggtc ggagccaagg ttggaggtca taatgattat gctatttttg aaattggcga
    13261 cgttcccttt attatcagtt agacgaccat cttccaaaac ttgtagcaaa atattgaaaa
    13321 cgtcactatg cgccttttcg atttcatcga ggaggataac tgaatagggt ttacggcgca
    13381 cggcttccgt aagttggcca ccttcttcat aaccaacata gccgggcgga gcgccgatga
    13441 gacgcgagac ggagtgtttt tccatatatt ctgacatatc aatgcggatc aaggcgtttt
    13501 catcattgaa aagaaattcg gctagcgctt tagccagctc ggttttgcca acgccggttg
    13561 agcctgtaaa gatgaaagaa gccaacggac gattttcatc ctgcaaacca gctcgagctc
    13621 gtcggacggc attactgaca gctttaatcg cttcatcttg tccgattact cgttcgtgca
    13681 gcttttcttc catttttaaa agtttttcac gttcaccctc gactaaacga gagaccggga
    13741 tatgagtcca tttggagaca atttcggcga tatcttcttc ggtaacctct tctcgcaaaa
    13801 gcgcccgttt accggaaaca tttttcaagg cttctttttc ggcttctaat tgtttgttta
    13861 gctcgactag ctcgtaatgt ttaagctggg cggctctttc ccagttgcct tcccgttccg
    13921 ctttctcggc ttcgagtttt ttcgcttcaa tttgcttctt taactcgctg atggcagtaa
    13981 tatgcttttt ttcattttgc cagcgagcgc ggagaacggc ggcttcgtct tttaactgcg
    14041 ccaattcctg ctgaattttt tcatacctct gccgggattc ctcaatatct ttttctttgg
    14101 cgagcgcccg actttcgatt tccaactggc taatcttgcg ttcgacttca tccagttcag
    14161 ccggcaggga atctatttca agacgcagac ggctagcggc ttcatcaatc aagtcaatgg
    14221 ctttatccgg tagaaaacga tcggtaatat aacgggctga taattctacc gccgccacaa
    14281 gagccgcatc ggtaatccta ataccgtggt gattctcgta tctttcctta agaccacgca
    14341 gaatcgaaat agaatcctca atggaaggtt catcaattaa aaccggctga aatcggcgtt
    14401 caagagcctt atctttttcg atatatttac ggtattcact gaaagtggta gccgcaatac
    14461 aacgcagatc accacgcgcc agagctggtt tcaataggtt ggaggcatca acagcccctt
    14521 cagccgcgcc ggctcccacg atcgtatgaa tttcatcaat aaacagaatg atctttccct
    14581 gagcttcgga aatttcacgc aggactgctt ttaagcgatc ctcgaattca ccgcggaatt
    14641 tggctccggc aatcaaagct cccatatcca gcgctaaaat acgcttgtct cggaaacttt
    14701 ccggcacatc ttgagaaaca atgcgcaagg ctaatccttc ggcgatagcc gttttgccga
    14761 cgccgggttc gccgataagc accggattat ttttcgtgcg gcgcgctaaa acctgcaaaa
    14821 cccgtcggat ttcttcctca cgtccgataa ccggatcgag tttcccgcgc cgggctaatt
    14881 cgttgagatc ccggcagtat tttttcaatg cctgatactt atcctcagga aattgatcgg
    14941 tgatagtttg agaaccacgg atttctttta aagctgacag aatggtttcg ttcgaaatac
    15001 catttttttt caataaattt tttaactcgc ctttagcaac ttctattatg cctaataaaa
    15061 gatgttcggc gctaagatat tcatcctgga gttgcttagc ctggtcttca gctacctgca
    15121 acattttatt ggaggcattg cttaaataag attggcttcc accactgact ttaggaaaag
    15181 tttccagaag attttcaaca ctacgagtga aaggttcaac gttcccgatc aatttgttta
    15241 gaactaattg accagtgttg tctggagtag tcagaattgc atacaggaaa tgttccggtt
    15301 caattgcttg attggaaagt cgctgagcag tattgatggc attttggacg atttcttgag
    15361 ctttgatggt aaatttttca cttgaataca ttttacatcc tccttagaat gcatagggaa
    15421 ttcctaattg gaatctcccc ggttgttttt tctcattctc tttagggatc ttgattgcat
    15481 aatcaatcct tattggcccc aaaggagtag caagagttag gccaaatcca taattccaac
    15541 ggtatgtctt aaaaaattca gaaaagtcag gttcgcctgc taagttgcca ccatcaacaa
    15601 aaacttctcc gccaaaaagc caaaacagcg gaaagcggac ttcaacgctg gttaagccct
    15661 taaaattata gccatcagga tcaatatggt caggttcaaa tttattatca ggccaagcgc
    15721 gtaaactggt agagccgcca agataaaatt tttcgtactc aggagtaggc tgtccggttc
    15781 tcaaaggcgc cataataccg acttttaccc gatatgccag aacgaccggt cccaagatat
    15841 tgaaatattg actgaaagat ggctccaatt tatagtaatc ggcgggcttg ccaccaacca
    15901 gagctatttt gccgcttaa
//
LOCUS       DAOMJU010000045        15879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_3594_length_15879_cov_17.614257, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000045 DAOMJU010000000
VERSION     DAOMJU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15879
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3594_length_15879_cov_17.614257"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLO58_05745"
     CDS             complement(<1..830)
                     /locus_tag="PLO58_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQO74327.1"
                     /translation="MLKVLIVDDNNESRSLLESFLNRHDYQVFSAVNGKLALDILENN
                     SIDLIISDILMPEMDGYMLCNIVKSDPRWKDIPLIFYSATFTSRQDEIFALKLGASRF
                     IRKPVESDLFLNIIQEVLQNRIEGSLEIAYPRFQDEKDAFKLYSERIIKKLEKNVSEL
                     EESEEHYRAIFENAAIGLYRSTPDGRILMANPTLVKMLGFDSFEELSKLNVEEEGFVL
                     KHPRSEFKKRIETEGKVQGLESVWKRKDNSLIHVRESAIAVRDSRGVTKYYEGTVEDI
                     T"
     gene            complement(843..1937)
                     /locus_tag="PLO58_05750"
     CDS             complement(843..1937)
                     /locus_tag="PLO58_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQO74328.1"
                     /translation="MENKKPTILIVDDVATNLNFLKGLLINDYDIKAANNGQLALKIA
                     RMGAGPDLILLDIMMPGMDGFQVCEELKRDPRTQDIPIIFLTARTATDDIVRGFKSGG
                     VDYLTKPFNPPELLARVRTHLLIKQQKDIILEQNREQKELLHILCHDLANHFTVISMS
                     LDLLKSDSAITDYLSKIKTATTHGIDIINLVREMRALQEKKIEISAVNLSECLAESVL
                     LLGDRFEQKNVKLLIDIDHSLWVMAERCSLINSVINNLMTNALKFSFEGGQVEISAAE
                     EDGAVIFKIQDHGIGMPAELMSQIFDITKITSRPGTKGEIGTGFGMPLVKSFVEFYGG
                     EIEVESRDIKKYADDHGTLITIKFINAKNN"
     gene            complement(1939..>5941)
                     /locus_tag="PLO58_05755"
     CDS             complement(1939..>5941)
                     /locus_tag="PLO58_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQO74329.1"
                     /translation="HDYSIRLPKTTQDEIGILYDGFNEMLEQIQKKQEERDKVEKKLR
                     EANTIINRSPVVAFTWYNEPTLPVEFVSESVINLFGYTADEFIQGKISYESCIYPEDL
                     PRVNSEVVKYSQTPDVQSFKHEPYRIVTKSGAIKWIDDLSFIIRSESGEITHYQGIVS
                     DITERMEFEELLRQSEIRYRRISSVVSDYVFSAKINQDGVLVTDWVGGAFETITGFTF
                     KEFSEAGGWRARLHPDDLEKDDHDFEKLKKNEPIQSELRFYTKSGELLWLRVYAYPVW
                     DYKNNRLIGVHGAVQNINERKKAEQALQESEARYRLLFESNPAPIIIYERNTYNILAV
                     NEAFLQFYGYSKDEILAMCLTDLYPADEKKKIVKMLNDLYDYQDAVEWHHLKKDGTMV
                     TVIFRSNDLKYQGHDARVAVITDVTERKLAEEKIKNLNIELEKRVTERTADLVKEIEE
                     REKIALTLEQSRESLRTIIESIPFPVVLVNQDRTIRGGNQATLDLLGFNSFDEIVGKN
                     CYEILCIYNSDTCPILSPDQIVDKKETVIINKDKVNIPVLKSAVPIKIEGEEVLLETY
                     VDILKIKIMEMELIQAKEQAQAATQAKSDFLANMSHEIRTPMNAIIGLTYLALQTELD
                     ARQHDYITKIKSSAQNLLEIINDILDFSKIEARRLKLENIDFNLEKVFQDTANVVTFK
                     AHQKNLEIIFGIDKNIPRYLVGDPLRLHQILANLTNNAVKFTDTGEIIVRAEMDEEYD
                     DTVKIKFSVKDTGIGLTKEQQDKLFQSFTQADSSTTRRYGGTGLGLAISKQLTELMQG
                     DIWVESVYGKGSTFYFTAVFKKQENQKIEEFVLTPDLRGLKVLVCDDNKSSRLIIKGA
                     LETFGFKVVLASSGKAAIEILKKHKSKPFQLIIMDWKMPEMDGIETIQTIRKDPEIPS
                     APAIIMVSAYSEEKIIDNIEKIGIDAFLLKPITNSTLFDTIMQVFGKTTPRRKRQADR
                     GQRLKSELALIKGARILVAEDNEINEQVINGLLTAYGMDVIVAENGRVAVQKCQEMHP
                     SELDLVFMDLEMPVLDGYEATRAIRQIAGYEKLPIVALTADAMSGTKGKVLSAMMNDY
                     ITKPVDPNEVYNILVKWIPAKRKKINKGESEQQLAKNLNFPEVSGIDIQSGLRRVAGN
                     KQLYGKILQNFVRENINLITSLQNYLVDNDLEAVEHLVHSLKGSSGNIGAQKLYEVAS
                     AFEMELKSKSPDSKNIMELIQQLSMELELVFNTIQKIELPAEIISSDKTNKPGKWGIE
                     QKKKCQELKKYLAEYDARASDIFYLIKSELSQSIPEVDLTKIAESIEQYDYDQALSIL
                     EKFIELGDQVNKD"
     assembly_gap    5942..6041
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6042..6710)
                     /locus_tag="PLO58_05760"
     CDS             complement(<6042..6710)
                     /locus_tag="PLO58_05760"
                     /inference="COORDINATES: protein motif:HMM:NF028461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HQO74330.1"
                     /translation="MKSINRGFFQKLSFRKKLIVIQLITVSTVIVFFIIFQVIINQIE
                     YQRDVNEKLETTARIIGSNSIPAILFLDNETAENILASLRSENEIVNAWLFDHNKNLF
                     ATFARDGFENYDYPFFDSGRHKSHSRDVIYSLELNQEEMPVGQILIRYKMHPLSLVII
                     ENLGLGVIVLLIGFGLALVLSFYTGKTVSNPIMALVDTIKEISTRHDYSIRLPKTTQD
                     EIGIL"
     gene            complement(6762..7304)
                     /locus_tag="PLO58_05765"
     CDS             complement(6762..7304)
                     /locus_tag="PLO58_05765"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HQO74331.1"
                     /translation="MPHKNKIIVCIVCFLILSFSFIYANLPFNSETEINDLKAAFIYN
                     FTKYITWPDLERNKVFFIGVIGNSDIVNSLRRVAEKKFVKNIPIRVAHYQSVDDLRYC
                     HILFISASEKNQLDPILAKLKKRKTLTIGDTPGFCESGVMINFFMQEDRLKFEMNLAK
                     LESVGLKASSQIKKLARIVE"
     gene            complement(7348..9300)
                     /locus_tag="PLO58_05770"
     CDS             complement(7348..9300)
                     /locus_tag="PLO58_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO74332.1"
                     /translation="MLKLRISIFAVSLFLISVTLAQDSELDEWLEMDLSDLMNIKVTT
                     ATKREQLIDKVPATVRIITDKEIQERAYHSLEDVLKDLPGFQFRDILGFNGYTFQRGA
                     PSQNNLILVMIDGIEINELNSGGFYGGYHYNLQNIKRIEVVYGPSSALYGTNAISGII
                     NLITKDAEDGPRAEASLTAGSFGTLFGDVGWRLKPENLPFSATFSAFYKQTEKADLGG
                     TKGDNNWSENMENFENDYGFDSKIKYKNTILGLTLQDKQASRTTNYKSLGTNYLDYGT
                     NFHIRFLNAYLSNLYTKKPSWSLKSQLYYRNSTVMDNTIAYITTDTGQVGYYRPNSLI
                     GLEEKFDYEGGSKANLVAGIVMEAEQLASDFSITYSDDPEKAPAKPEEPDYENNTLIS
                     FYVQTQYKFWELVEMTAGARLDQSSYYGTILTPRLSFVYSHKNYTGKLLYTEAFRAPR
                     PWDYNWGIGNPNLKPERMQSIELANNLNFNQYLRCDFSLFHNQIYDMLIEKDSCWVNA
                     ERINTNGLELNLDYRRKKMQLNLNYTFTDSKFSDGKQVPEIARHGFNAGVTYSLLTNL
                     NVNLRGNYLSRRKNPVFISTTKSHYVDPYFVLNSVINYNPIPNLKLQIAVNNLLNARY
                     YHTSNRPPERYRQPQRAFLFKLGYQL"
     gene            complement(9464..10909)
                     /locus_tag="PLO58_05775"
     CDS             complement(9464..10909)
                     /locus_tag="PLO58_05775"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HQO74333.1"
                     /translation="MEQLKINQISEQVWEIPPTGAMRVPGRIYANENLMQVIRDDQSL
                     KQVANVAQLPGIVGYSMAMPDIHWGYGFPIGGVAAFDIKEGVISPGGVGYDINCGVRL
                     IRTNLVADQLKIDIKNLIAAIFNSVPSGIGSHGAIRKLSRRELERVAVQGAKWAIEMG
                     YGSADDLQFIEDRGCLADAEPSVISQRAFERGADQLGTLGSGNHFIEIGKVVDIYDST
                     AAQAMGIFQGNLVLILHTGSRGFGYQICDDFIKSTLRATQKYEIKIPDRQLACAPLKS
                     IEGQDYLGAMRAAANFAFANRQVISSLIYDSLLKTLKISPNELGFRPVWDIAHNIAKI
                     EEHQYGGKKIQVCVHRKGATRAFGPGNPELPEIYQAIGQPVLIPGDMGTASYLCVGTE
                     IAMRETFGSACHGAGRILSRHQAKAKARSLDLYQALAESGVYVMAEEKGTIAEEMPFA
                     YKDVGEVVDSVDAAGIVRKVARFKPLGVIKG"
     gene            complement(10919..11362)
                     /locus_tag="PLO58_05780"
     CDS             complement(10919..11362)
                     /locus_tag="PLO58_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HQO74334.1"
                     /translation="MSKSERARKSKFYRIFPHTADLGVEVYGANLLELFEHSALALFD
                     LMANVAKVTEQKTCEFSVEAADIELLLREWLGELLYYSISRQFLFKSCHIKQFGEQTL
                     IASASGEPFNPQKHILKREIKSVTYHELQIKNQGNHWKARFVLDI"
     gene            complement(11584..12423)
                     /locus_tag="PLO58_05785"
     CDS             complement(11584..12423)
                     /locus_tag="PLO58_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQO74335.1"
                     /translation="MLKEDLIYRGKSKDVVEIKSGPYTGKYRLIFTDRATGYLENGKP
                     VFDPGYDNVVGEIPGKGAIACRFATHFFKLLKERGIPSHYIDTVSDNEMIVEPAVPLS
                     MPAESPEFPGAAPLLNLEFTWRNNATGSFWRRYPFVRPCRNIHTIVEAWTKGDSDILI
                     TFEALAETGAMTKDEIKQSIKLVKEIADIVAQEFASNNLHVIDGKFELGRLKYGDGKI
                     VIIDEISPDVLRVCKGYSPDADGNCTVYKNCTVTSYADGKRTIKNKNQVQAVDFVNIF
                     LSH"
     gene            complement(12648..13559)
                     /locus_tag="PLO58_05790"
     CDS             complement(12648..13559)
                     /locus_tag="PLO58_05790"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HQO74336.1"
                     /translation="MAKMQWYKGSLHTHAREGESDSSVEYVVKWYREHDYNFLVITDH
                     STVTIPPAYAIEGDPSFLFIPGEEIMGYGTGRLLEINAFNITEDISPIHSETDLDALQ
                     RCLDTVREIGGVPMINHPNYQWRLDADIILNSKDCNLFELYNGCPGTNNYGDDNHPST
                     EQIWDFMLSAGKRIYATATDDAHVYQKFSAELSNPGRGWVVVKAKKLEVNEILQNLEA
                     GLFYSSTGVEISDLIVKPVQIEIFIQMSGKNQYTTDFIGTKGKLLLRTQNNPAVYKLT
                     SNEKYVRAKIVASDGSCAWIQPIFVVP"
     gene            complement(13774..13846)
                     /locus_tag="PLO58_05795"
     tRNA            complement(13774..13846)
                     /locus_tag="PLO58_05795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13810..13812),aa:Arg,seq:ccg)
     gene            13983..14999
                     /locus_tag="PLO58_05800"
     CDS             13983..14999
                     /locus_tag="PLO58_05800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQO74337.1"
                     /translation="MKRILPIILVGFLVFSCAQKEKSAKLEKGTDLYQLAQELSQQIP
                     ILDPEKNTVIVTTKKFDITVGDVFTTLHQLYGDGIQQLKSLDTNQLTEIIKGNAIALA
                     EKKLLLQAAEKNGIKISDAEVDSILELQYMQVGGKEKFLNYLSNANINFKALREDIRD
                     NLAINSYLDKMIMDSVNITEEDIQKYYNETDQTATVRHILKITQGKSPEEKAQIHSEM
                     EGILDRAQKGENFESLAKKYSDDPGSKDNGGLYKDFGRGEMVKPFEDAAFDLPIGSIS
                     DIVESPYGYHIIKVIDRKKESRPLDTVRSEIENILINSRQNELLINLLDNLKKENDFK
                     VVIP"
     gene            complement(15072..15410)
                     /locus_tag="PLO58_05805"
     CDS             complement(15072..15410)
                     /locus_tag="PLO58_05805"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQO74338.1"
                     /translation="MIIRKVNEVLSEPVAEPGAENVRRQVLLSPSEGAPHFTLRRFEI
                     APGGQTMYHHHNYEHEIYVLAGVGKARGRNSEVRINQDDAILVLPNEEHQIVNTGITP
                     LIFLCLIPNQ"
     gene            complement(15388..>15879)
                     /locus_tag="PLO58_05810"
     CDS             complement(15388..>15879)
                     /locus_tag="PLO58_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HQO74339.1"
                     /translation="AGFRHYEISNFAKPGFEARHNSIYWKGGRYFGFGAAAHSYDGQK
                     RWWNKRLIDDYIKALQNNCLPVAGQEKLSLSDRITEAVFLGLRTAEGLDIALLEKFSG
                     IEFAHIYSKLINKIGDNPEQLFALQEGHLILTTRGWFLCDSIIEKILTIIEETKNDNT
                     KSK"
BASE COUNT         4660 a         3384 c         2829 g         4906 t
ORIGIN      
        1 gtaatatcct cgacggtgcc ttcataatat ttggttacac cccttgaatc tcgaaccgca
       61 atagcgcttt cgcgaacgtg aatgagcgag ttatctttac gcttccaaac tgattctaaa
      121 ccctgaacct ttccttcggt ttcgatccgt tttttaaatt ccgaccgcgg gtgcttgaga
      181 acaaatcctt cttcttcgac gttcagcttt gataattcct cgaaagaatc gaagccaagc
      241 atttttacca aggtcgggtt tgccattaaa attctgccat ccggagtaga acgatagagc
      301 ccaatcgccg cattttcaaa aatggctcta taatgctcct cactttcttc aagttcactt
      361 acattttttt ccaatttctt gattatgcgt tcactatata acttgaaagc atctttttca
      421 tcttgaaacc ttggataggc gatttccaga ctcccttcaa ttcggttttg cagaacctct
      481 tgaataatat tcagaaaaag gtccgattca accggtttac gtataaatcg cgaagcacct
      541 aatttcagag cgaaaatctc atcttgtcgt gaagtaaaag tagccgagta gaaaattaat
      601 ggaatgtcct tccagcgcgg atcagattta acaatattgc ataacatata gccatccatc
      661 tctggcatca aaatatccga aatgattaaa tcaatgctat tattctccaa aatatccagc
      721 gccaatttgc cgttaaccgc agaaaatacc tggtagtcat gacggttaag aaacgactct
      781 aatagggaac gactctcatt attgtcatca acaatcaaca ctttcagcat aaattgctcc
      841 tcctaattat ttttagcatt aatgaattta attgtgatta aagtaccatg atcgtcggcg
      901 tatttcttaa tatctcgcga ctccacctcg atttcgccac cataaaattc gacgaaagat
      961 ttaactaatg gcatcccaaa accggttcca atttcgcctt tagtcccagg gcgacttgta
     1021 attttagtta tatcaaaaat ctggctcatc aactcagctg gcatgccaat tccatgatct
     1081 tggattttga aaatcacggc gccatcttct tctgccgccg aaatttcgac ctgccctcct
     1141 tcaaaggaaa atttcagggc attcgtcatc aaattattaa taactgaatt gattaaagaa
     1201 catctctctg ccattaccca taggctatgg tcaatatcta tcaacagttt aacatttttc
     1261 tgctcaaagc gatcaccaag tagcaaaacc gattctgcca aacattcgct caggttcacc
     1321 gcacttattt ctattttttt ctcctggaga gcacgcattt ctcggactaa attaatgatg
     1381 tcaataccat gggtagtcgc agtttttatt ttactgaggt aatcggttat agctgaatca
     1441 cttttcagta aatcgaggga catacttata acagtaaagt gattggcaag gtcgtgacat
     1501 aaaatatgta ataattcttt ctgctcacgg ttctgttcga ggatgatatc tttttgttgc
     1561 ttaattaaaa gatgggtacg gacgcgcgcc agtagctcgg gcggattaaa cggcttagtc
     1621 agataatcaa ctccaccgga tttaaagcct cttacaatat catcggttgc cgttctggca
     1681 gtcagaaaaa taattggaat atcctgagtt cgcggatcac gctttagctc ttcacaaact
     1741 tggaatccat ccataccagg catcataata tcgagcagaa tcaaatcggg tcctgctccc
     1801 attcgagcga ttttcagtgc taattgaccg ttattggccg cttttatgtc gtaatcattt
     1861 attagtagcc ctttcagaaa attcagattc gttgctacgt catcaacaat taagattgta
     1921 ggttttttat tctccatatt aatccttatt gacctggtcg cccagttcaa tgaatttctc
     1981 taaaatactt agggcttgat cataatcgta ttgctcaata gattcggcga tttttgtcaa
     2041 atctacttca ggaattgatt ggcttaattc actcttgatc aaatagaaaa tgtcactggc
     2101 tcttgcgtca tactcagcca agtatttttt taattcctgg catttctttt tctgctcaat
     2161 tccccatttc ccgggtttat tggttttatc agaactaata atttcggctg gcaattcaat
     2221 tttttgaatt gtattaaaaa ccaactcaag ttccatactc aattgctgaa ttaattccat
     2281 aatattttta gaatcaggag attttgattt taattccatt tcaaacgcag aagcgacttc
     2341 atataatttc tgggctccga tattaccgga acttcctttg agagaatgta ccagatgttc
     2401 aactgcttct aagtcattat ctaccaagta attttgaagg cttgtgatca gatttatatt
     2461 ttcacgcaca aaattttgca gaatctttcc gtataattgt ttattaccgg cgacccgtct
     2521 caaaccagat tgtatatcaa taccagatac ttctggaaaa tttagatttt tagccaactg
     2581 ctgttcagat tcacctttat ttattttttt tcttttagct ggaatccatt taaccagaat
     2641 gttatagacc tcgttcggat caaccggttt ggtaatgtaa tcattcatca tcgccgataa
     2701 tactttccct tttgtgccgg acatagcatc ggctgtcagc gcgacaatcg gtagcttttc
     2761 atatcccgca atttgacgga tcgccctggt cgcttcataa ccatccaata ccggcatctc
     2821 aagatccata aaaactaaat cgagttcaga aggatgcatt tcctgacatt tctggacggc
     2881 gacgcggcca ttttcggcta cgataacgtc cattccataa gcagtaagca atccatttat
     2941 aacctgctca ttgatttcat tatcttcggc aactaagatc cgggcgcctt tgatgagcgc
     3001 caactcactt ttaagtcttt ggcctcggtc ggcttggcgt ttgcgcctag gagtggtttt
     3061 gccaaagacc tgcataattg tatcaaataa tgtcgaatta gtgatcggtt ttaataaaaa
     3121 tgcatctatt ccgatttttt caatattgtc aatgattttt tcttcactat aagcgctgac
     3181 catgatgatg gctggagcag agggaatttc aggatccttt cggattgttt gaatagtctc
     3241 gataccatcc atttcaggca ttttccagtc cattataata agctgaaatg gtttgctttt
     3301 gtgtttcttt aaaatttcta tagccgcttt gccggaagaa gccagcacga ctttaaaacc
     3361 gaaagtctca agtgcgcctt tgataattaa acgagaactt ttattatcgt cgcaaaccag
     3421 aacttttaac cctcgtaaat cgggagtaag aacaaattct tcgattttct gattttcctg
     3481 ctttttaaag acggcggtaa agtagaaggt actgcctttt ccataaacac tttcgaccca
     3541 aatatcgcct tgcatcagtt cggtcaattg cttagaaata gccaatccca gaccggtacc
     3601 accataacgt ctggtagtgg aggaatccgc ctgggtaaaa gattgaaaga gcttatcctg
     3661 ctgttcctta gtaagaccaa tgccggtgtc tttaactgag aactttattt ttactgtgtc
     3721 gtcatattcc tcatccattt ctgcccggac aataatctcg ccggtatcag tgaacttgac
     3781 ggcattatta gtcaaattcg ccagtatttg atgcagacgc aagggatcgc caactaagta
     3841 gcgcggaata tttttatcta tgccgaagat gatttccaga tttttttgat gggctttaaa
     3901 ggttacaaca ttggcggtat cctgaaaaac tttttccaga ttaaaatcaa tattctccag
     3961 ttttagtcta cgagcttcga tcttggagaa gtccagaata tcattaatga tttccagcaa
     4021 attctgagcg gatgatttta ttttagttat gtaatcatgc tgtcgagcgt ctaactcggt
     4081 ttgtaaggct aaatacgtta aaccgataat cgcattcatc ggagttctta tttcgtggct
     4141 catattagcc agaaagtcac ttttggcttg ggtagcggct tgggcttgtt ccttagcctg
     4201 aatcagttcc atttccataa ttttgatttt gagtatatca acataggttt ccagcagtac
     4261 ctcttcccct tcgattttaa ttggaactgc actttttagt actggaatgt ttaccttatc
     4321 tttatttatg atcactgttt cttttttatc tactatctga tctgggctta agattgggca
     4381 agtatcgcta ttataaatac ataaaatttc ataacaattt ttcccaacaa tttcatcgaa
     4441 cgaattaaac ccgagtaagt caagagtagc ctgattacca cctctgattg ttcgatcttg
     4501 attaaccagc actacaggaa atggtatgga ttcaatgata gttctcagag attctcggga
     4561 ttgttccaga gtcagtgcaa ttttttcccg ttcttcaatt tcttttacta aatcggcagt
     4621 tcgttcagta actctttttt ccagctctat gttcaaattt ttgatttttt cttcggctaa
     4681 ttttcgttca gtaacgtcag tgataactgc gacacgggca tcatgcccct gatatttcaa
     4741 atcattcgaa cggaaaatga cggttaccat tgttccatct tttttcaagt gatgccattc
     4801 gacagcatct tgataatcat ataaatcatt tagcatttta actattttct ttttttcatc
     4861 tgccggataa agatccgtca agcacattgc cagaatttca tctttggaat aaccataaaa
     4921 ctgaaggaaa gcctcattaa ccgctaaaat attataggta ttacgttcat agattataat
     4981 cggcgccgga ttagattcga aaagcaatcg gtaacgggct tcgctttcct gaagagcttg
     5041 ctcagctttc ttccgctcat tgatattttg aaccgcgcca tgaacaccaa ttaatctatt
     5101 gtttttataa tcccaaaccg gataagcata aactcttaac cataatagtt caccggattt
     5161 agtataaaag cgtaattcgg attggatcgg ttcattcttt ttcaattttt caaaatcatg
     5221 atcatctttt tcaagatcgt ccggatggag cctggctctc caaccaccgg cttcactaaa
     5281 ttccttgaag gtaaatccag ttatggtctc aaaggcaccg ccaacccagt cggttaccag
     5341 aacaccatct tgattgattt tggccgaaaa aacataatcc gatactacgg aagatattcg
     5401 acgataacgg atttcgctct gcctcagcaa ttcttcgaat tccatacgtt cagtaatatc
     5461 agaaacgatg ccctgataat gggtaatttc tccggattca cttctaatga taaaagatag
     5521 atcatcaatc cactttattg cgcctgattt agtaacaatc cgataaggtt catgtttaaa
     5581 agattgcacg tcgggtgttt gactatactt aactacctca ctattaactc tcggtaaatc
     5641 ctcaggataa atacatgatt cataggatat tttgccttgt ataaactcat cggcggtata
     5701 gccaaaaaga ttaatgacac tttcagaaac aaactcaact ggcaaggtag gttcgttata
     5761 ccaggtaaaa gctacgaccg gactgcgatt gataattgtg ttagcttctc tgagtttttt
     5821 ttcgacttta tccctttctt cctgtttttt ctggatttgc tccagcattt cgttaaatcc
     5881 atcatagagt attccgattt catcttgagt cgtttttggt aaacggattg aataatcatg
     5941 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagtattcc gatttcatct
     6061 tgagtcgttt ttggtaaacg gattgaataa tcatgtcgtg tcgagatttc tttaatcgta
     6121 tcaaccaatg ccataatagg gtttgaaacg gtcttgccgg tataaaatga aagaactaaa
     6181 gctagtccaa aaccaatcag caaaacgatt accccaagtc ctaaattttc tattattacc
     6241 agacttaaag gatgcatttt atagcgaatc aaaatttgcc cgaccggcat ttcttcctgg
     6301 ttaagttcca aagaataaat tacatcgcgc gaatgagact tatgtctacc gctatcaaag
     6361 aaaggataat cataattctc aaacccatca cgtgcaaatg ttgcaaacaa attcttattg
     6421 tgatcaaata accaagcatt gacgatttca ttttcacttc gtaaggaagc cagaatattt
     6481 tcagccgttt cgttatcaag gaataaaata gcaggaatag aattcgagcc aatgatgcga
     6541 gctgtcgttt ctaacttttc attgacatct cgctgatact caatctgatt tattattacc
     6601 tgaaaaataa tgaaaaatac gataacagtg cttactgtaa ttaactgaat tacaatcagt
     6661 tttttcctaa atgacaactt ctgaaaaaat ccccgattga tagacttcat cttcgccata
     6721 gcatcatacc aaataattta atttggcttt aaatgaaggc atcattcaac aatccgggct
     6781 aattttttaa tttgggaact ggctttcaag ccgacacttt ccaatttggc aagattcatt
     6841 tcaaatttta acctatcttc ttgcataaaa aaattaatca ttacaccact ttcacaaaat
     6901 ccgggagtgt caccgatcgt caaggtcttc cttttcttaa gtttggctaa tattggatcg
     6961 agttgatttt tttcagatgc agaaatgaat aaaatatggc aatatctcaa atcatcaaca
     7021 ctttggtagt gagcaactct aattggtata tttttaacga attttttctc agccactcgt
     7081 cgcagggaat tgacaatatc actattgccg atcactccga taaaaaatac tttattacgt
     7141 tcaagatctg gccaggtaat atattttgta aaattataaa tgaaagccgc ttttaagtca
     7201 ttaatctcag tttcactatt gaatggcaaa ttggcgtaga taaaggaaaa actaagaatc
     7261 aggaagcaaa caatacagac gataatttta tttttatgcg gcattcaata gatcaatttt
     7321 ctttggaata catcaaattt catcatatca aagttgatac cccaatttga acaaaaaagc
     7381 tcgttgtggt tgacgatagc gttctggcgg tcggttacta gtatggtagt agcgagcatt
     7441 taataaattg ttgacggcaa tctgcaattt cagatttggg atcgggttat agttgataac
     7501 actattgagc acaaagtatg gatcaacata atgactttta gttgtagaaa taaaaacggg
     7561 atttttacgg cggctgaggt aattaccacg cagattgaca ttgaggttcg tcaataacga
     7621 ataggtaacc ccagcattga atccgtggcg ggcaatttca gggacctgct tgccgtcaga
     7681 aaatttagaa tctgtgaaag tataattcag gtttagctgc atttttttcc ggcggtaatc
     7741 aagatttaat tccagaccat tagtatttat gcgttcggca tttacccaac atgaatcttt
     7801 ctcaattagc atatcataaa tttgattatg gaatagcgaa aaatcacagc gtaagtattg
     7861 attgaaattt agattattgg ctaattcaat tgattgcatt ctttccggtt tcaggttcgg
     7921 attaccaatg ccccagttgt aatcccaggg cctcggagcg cgaaaagctt cagtataaag
     7981 taacttgccg gtataattct tatggctata aacaaaactt aggcgaggag tcaaaatcgt
     8041 cccataatag gaactttgat caaggcgtgc tccagccgtc atttcgacta attcccaaaa
     8101 tttgtattgg gtttgaacat agaaacttat caaagtatta ttttcataat ccggttcctc
     8161 tggtttagcc ggagcttttt ctgggtcatc actataggtt atagaaaagt cgctggcgag
     8221 ctgttcggct tccattacaa ttccggcaac caagttggct ttactgcccc cttcataatc
     8281 aaatttttct tccagcccga ttagtgaatt cggacgataa tatccaacct gaccagtatc
     8341 agtagtaata taggcaattg tattatccat taccgtcgag tttctataat ataattgcga
     8401 tttcaatgac cagctcggtt ttttagtgta caagttgctc aaataggcat tcaaaaagcg
     8461 aatgtgaaaa ttcgtaccat agtcaaggta attagtgccg agcgatttat aatttgtcgt
     8521 gcgggaagcc tgtttatcct gaagtgtcaa accaagtatg gtgtttttat atttgatttt
     8581 ggaatcaaat ccataatcgt tttcaaaatt ttccatattc tctgaccaat tgttgtcgcc
     8641 tttagtaccc cccaaatccg ccttttcagt ttgtttataa aaagcggaaa aagttgcgct
     8701 gaacggcaaa ttttccggct ttagacgcca accgacatcg ccgaaaagcg ttccaaatga
     8761 accggctgtt aggctcgctt cggctctcgg tccgtcttcc gcatctttgg tgattaaatt
     8821 tattattccg gaaatggcgt tcgttccgta taaggctgat gaaggtccat aaaccacttc
     8881 tattcgtttg atattttgca aattataatg ataaccgccg taaaatcctc ccgaattcaa
     8941 ttcattgatc tcgatcccat caatcatcac taaaattaga ttattctggc tgggcgcgcc
     9001 gcgctgaaat gtgtaaccgt taaaacctaa tatatcgcga aattgaaatc cgggcagatc
     9061 ttttagaaca tcttccagac tgtggtaggc tcgctcttga atttccttat cagtaataat
     9121 ccggacggta gcgggcactt tatcaatgag ttgctccctc ttggtagcag tcgtgacttt
     9181 gatattcatc aaatcactta aatccatttc tagccattca tctaattcag aatcctgcgc
     9241 tagagtaaca ctgataagaa ataaagaaac ggcaaagatg ctaattctca actttaacat
     9301 aacagtgccc ttttgagaca attgttacaa atttttgaga ataagtcatt ttctttatta
     9361 aatagccacc aatcctatat taatcaagaa aaatcgcaat gtcaatgcaa atgcgataat
     9421 cactcgctaa atcaacgact tggtgccatt gcgtttatca cttttatcct tttattacgc
     9481 ccagcggttt gaagcgagcg actttacgaa cgataccggc cgcatctacc gaatcaacca
     9541 cttcgcccac atctttatat gcaaatggca tttcttcggc gatagttcct ttttcttccg
     9601 ccattacata gactccactt tccgctaatg cctgatataa atcaagactg cgggctttgg
     9661 ctttggcttg atgccgcgat aaaattctac cagccccgtg gcaggcacta ccaaaagttt
     9721 ctcgcatagc aatctctgtc ccaacgcata aataactcgc cgtgcccata tcaccgggaa
     9781 ttagtaccgg ttgaccaatt gcttgataaa tttctggtaa ttcaggatta ccgggaccga
     9841 aggcgcgggt tgcacctttg cgatgaacgc aaacctggat ttttttcccg ccatattgat
     9901 gttcttcaat cttagcgata ttatgcgcaa tatcccagac tgggcgaaaa ccgagttcgt
     9961 tcggactaat ttttaaggtt ttcaaaaggc tatcataaat caacgagcta atcacctgac
    10021 ggttagcgaa ggcaaaattc gccgcggctc gcatagctcc gagataatct tgcccttcta
    10081 ttgattttaa tggggcgcaa gccaactgcc tgtcaggaat ttttatttca tatttttggg
    10141 tggcacgtag agttgatttg ataaaatcat cgcaaatttg gtaaccgaaa cctcgcgaac
    10201 cggtatggag aattaaaaca agattgccct gaaatatacc catcgcctgc gccgccgttg
    10261 aatcgtatat atccactact tttccaatct cgataaaatg attaccactg ccgagcgtcc
    10321 ccagttgatc ggcaccccgc tcgaatgcgc gttgcgatat tacggacggt tcggcatccg
    10381 caagacagcc cctatcttca ataaactgta aatcatcggc acttccgtaa cccatttcta
    10441 tcgcccattt agctccttga actgccaccc gttcgagttc acggcgactg agtttccgga
    10501 tagctccatg cgagccaatc ccactgggaa cactattgaa aatagcggca atcaagtttt
    10561 taatgtcaat tttgagctgg tcggcaacca gatttgtcct tatcaaacgc accccgcagt
    10621 taatatcata gccgactccg cctggagaaa tcacaccttc tttaatatca aaagccgcta
    10681 ctcctccaat cgggaatcca taaccccagt gaatatcggg catagccatg gaatagccta
    10741 cgattccggg taattgagct acatttgcga cctgtttaag gctctgatca tctcgaataa
    10801 cctgcatcag gttttcattc gcatagattc gcccaggaac gcgcattgcg ccggttggcg
    10861 gtatttccca tacttgctcg cttatttgat ttatttttaa ttgctccatt actaaccttt
    10921 aaatatcgag gacaaagcgc gctttccagt gattaccttg atttttaatt tgtaattcat
    10981 gataagtaac gctctttatc tcacgtttta aaatatgttt ctgcgggttg aacggttcgc
    11041 cactcgcgct ggctattaat gtttgctcgc caaattgttt gatgtggcaa gatttaaata
    11101 agaattgccg gcttatagaa taataaagca attcgccgag ccattcccgc agaagtaatt
    11161 ctatgtcagc ggcttctact gaaaattcgc aagttttttg ctccgtaact ttcgccacat
    11221 tagccatcaa atcgaacaaa gccaaagcgg aatgttcgaa aagttccagc aaattggcgc
    11281 cgtacacctc cactcccaga tcagccgtgt gcggaaaaat ccggtaaaat ttgctttttc
    11341 gtgcccgttc cgatttactc aaatcaactt ccatatttat aattttaatc ccacatttct
    11401 ccataaccta aagtaagata tacactatta gagacaagag caaatttgct gttaattaaa
    11461 attaaaaata ggtgattaat tcaatagtcg agaatcagag aattttatag aactgcgccc
    11521 atattctctt atttcttaaa agagcttact agcacttaaa agaactcaaa tacgcttatt
    11581 cactcaatga ctgaggaaaa tattgacaaa atcaaccgct tgaacctggt ttttattctt
    11641 gatagttctc tttccatcgg cataactcgt cacggtacaa tttttataaa ccgtgcaatt
    11701 gccgtcagca tcgggagagt agcccttgca aacccgcaga acatcgggag agatctcgtc
    11761 aataattaca attttcccat cgccatactt cagtcgcccc aactcaaatt tcccatcaat
    11821 gacatggagg ttgttgctgg caaattcctg tgcaacgata tcagcgatct ctttaaccag
    11881 tttaatactc tgcttaatct cgtctttagt catagcaccg gtttccgcta acgcttcaaa
    11941 cgtaatgaga atatcactat ctccttttgt ccaggcttca acgatcgtat gaatattgcg
    12001 gcaaggtcgc acaaaaggat atcttctcca gaaactacca gtggcgttat tccgccaggt
    12061 aaattcaaga ttcaagagtg gagccgcccc gggaaattcc ggtgattcgg ctggcatact
    12121 aagcgggaca gccggttcga cgatcatttc attatcgctg acggtatcaa tataatggga
    12181 aggaataccc ctttccttta gcagtttaaa aaaatgcgtg gcgaatcggc aagcaatagc
    12241 gccttttcca ggaatttcgc cgacgacgtt atcatatcca ggatcaaaaa caggtttacc
    12301 attttccaga taacccgtag ctctatcggt aaaaattaaa cgatatttac cagtgtatgg
    12361 cccacttttt atttcgacga catctttgga tttcccacgg taaatcaaat cttctttcag
    12421 cataagtact tttccttttg taacaattgt aaatcaaaaa caatcaatat caagtttagc
    12481 aactgataat ttacaacaat ccgaaaaact gaccattaaa atatcaagcc tctgtcaatc
    12541 caccaaaatt tgcccattat tttggataat aattgcgagc gtcagtatca attaaacttt
    12601 agatctttgc ttttattaat aagccctgcc taatttttca gccgaaatta tggcacaaca
    12661 aatatcggct gaatccaggc acaactccca tcggaagcca caatttttgc ccgtacatat
    12721 ttttcattag aagtcaattt atagactgcc ggattatttt gcgtgcgtag caggagtttg
    12781 cccttggtac cgataaaatc agttgtatat tggtttttgc cactcatctg gataaagatt
    12841 tcgatttgga caggtttgac gattaaatca gaaatttcga cgccagtgct tgaataaaat
    12901 aaaccggctt cgagattctg caaaatttcg tttacctcaa gttttttagc tttcaccacc
    12961 acccatccac gacccgggtt agataattcg gcagaaaatt tttgatatac gtgagcatca
    13021 tcagtagcag tagcataaat tcgctttcca gccgatagca taaaatccca tatctgctcc
    13081 gtgcttggat gattatcatc accataattg ttggtaccag gacaaccgtt atataactca
    13141 aataaattgc agtccttaga attcaatata atgtctgcgt cgagtcgcca ttgataattg
    13201 ggatgattta tcatcggaac gcctcctatt tcccgaaccg tgtcaagaca tctttggaga
    13261 gcatctaaat cggtttcgct atgaattggc gaaatatctt cggtaatatt aaaggcatta
    13321 atctccaaca aacggccagt tccgtatccc attatttctt cccccggaat aaatagaaag
    13381 gagggatcac cttcaatggc gtaggccggt ggaatagtta cagtcgagtg atcggtaata
    13441 accaaaaagt tataatcgtg ttcccgatac catttcacaa catactcaac cgaggaatcg
    13501 ctttcacctt ctcgggcgtg agtatgaaga ctacccttgt accattgcat cttagccata
    13561 tcgcgtaatt caaattgctg gctgaacaac tggaagcgta agaacagtac tattgcaaat
    13621 agcagtaatg actttctctt aaaatttttc tttaaacaac cgagaccaaa cacgccaata
    13681 atatatttaa aaccggctta ctataaaagt ttagcttttg gaaaaaggtt aatgaactta
    13741 caatatggca agattttttt aacggagact tagtaccccc aacaggactc gaacctgtgc
    13801 aaccggctcc ggaggccggt gctctatcca tctgagctat gggggcttca ataagccttt
    13861 taagttacat ctaacattcc aatttctcaa aaagaattcc ttgacaaata attatgaatt
    13921 aattcaattt ttatctaatt ttaatgcgtt tttagaaata tttctaagta aaggagaaaa
    13981 tgatgaaaag aatattgccg atcatattag taggattttt agtcttttct tgtgcccaga
    14041 aagaaaaaag cgccaaatta gaaaaaggaa ccgatcttta tcaattagcc caggaactat
    14101 cccagcaaat tccgatactc gatcctgaaa aaaataccgt aattgttact accaaaaaat
    14161 ttgatattac cgtcggagat gttttcacca ctttacatca gttgtacgga gatggcattc
    14221 agcaattaaa aagccttgat accaatcaat tgactgaaat aatcaaggga aacgctattg
    14281 cgttggctga gaaaaaactc ctcttacaag cggctgagaa aaatgggata aaaatatcag
    14341 acgccgaggt tgacagcatc cttgaattac agtacatgca ggtcggcggt aaggaaaaat
    14401 ttcttaatta cttaagcaat gccaatatta attttaaagc cttacgggaa gatatccgtg
    14461 ataatctggc tatcaatagc tatctcgaca aaatgataat ggactcggtt aatattacag
    14521 aagaagacat tcaaaagtat tataacgaaa ccgatcaaac cgctaccgtt cgacatattt
    14581 taaaaatcac gcagggaaaa tcgcctgaag aaaaagccca gattcattcc gaaatggaag
    14641 gaattcttga tcgagcccaa aaaggtgaaa atttcgaatc tctggctaaa aaatactcgg
    14701 atgacccagg ctcaaaagat aacggcggat tatataaaga cttcggccgc ggcgaaatgg
    14761 taaaaccttt cgaggacgcc gcttttgatt taccaatcgg cagtatcagc gatattgtcg
    14821 aaagtcctta tggttaccat attattaaag ttatcgaccg taaaaaagag agccgtccgt
    14881 tggataccgt ccggagtgaa attgaaaata ttttaatcaa ttcgcgccag aatgaattac
    14941 tgataaactt gctggataac ctgaaaaaag agaacgactt taaagtcgtg ataccataag
    15001 aatatcaata aatactttac aaaggagctg aaatttcagc tcctttttat tttatctgct
    15061 taatttaaaa gttactgatt cggaatcagg cacaaaaata tcaatggggt aatacctgta
    15121 ttaacaatct ggtgctcttc attaggcaaa accagaatcg catcatcttg attgattcgg
    15181 acttcggaat tcctcccacg cgctttacca actcccgcca gaacatagat ttcgtgttca
    15241 taattgtggt gatgatacat agtttgtcca ccaggagcaa tctcgaatcg gcgcaaggta
    15301 aaatgtggcg caccttcact cggggaaagt agtacctggc gacgaacatt ctcagcgccc
    15361 ggttcagcga ccggttctga tagcacttca tttacttttc gtattatcat tctttgtctc
    15421 ctcgatgatt gttaaaattt tttcaataat cgaatcgcag agaaaccacc cacgcgtcgt
    15481 cagaattaaa tgtccctctt gcaaagcaaa taactgctcg ggattgtcgc caatcttatt
    15541 tattaatttg ctataaatat gcgcgaattc gatgccggaa aatttttcca ataacgcaat
    15601 atccaaacct tcagcggtac gtaatcctaa aaaaaccgct tcggttatgc gatcggaaag
    15661 acttaatttt tcctgaccgg caaccggcag acagttattc tgcagggctt taatataatc
    15721 atcaatcaaa cgtttattcc accagcgttt ttgcccgtcg tatgaatggg cagcggctcc
    15781 gaatccaaag tatcttcctc ccttccagta aatcgagtta tgtcgcgcct cgaaaccagg
    15841 tttggcaaaa tttgaaattt cataatgccg aaaaccggc
//
LOCUS       DAOMJU010000046        15708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_3653_length_15708_cov_23.269022, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000046 DAOMJU010000000
VERSION     DAOMJU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15708
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3653_length_15708_cov_23.269022"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /gene="asd"
                     /locus_tag="PLO58_05815"
     CDS             complement(<1..1070)
                     /gene="asd"
                     /locus_tag="PLO58_05815"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQO74340.1"
                     /translation="MKSNSQLKSKIPVGILGATGSVGQKFIELLTEHPWFEIAALAAS
                     DRSAGKTYRDAAHWFMTKPLPDNVAKMIVKNCEPNLPCRIVFSGLDASVAGEIETDFA
                     KAGYIVISNSRNHRYDPDVPILVPEVNSDHLAIIATQKYGRGAIITNPNCAATGLVVA
                     LKPLLDQFGLSAVNIVTMQAISGAGYPGVASLDIVDNVIPFISGEEAKLESEPQKILG
                     KYLDGHIEPAQFIISAACNRVAVVDGHTECVSVKLERKPTAGEIIAAWREFKGEPQRL
                     KLPSAPEKPLCYFEESSYPQPRLHRLLENGMTVSLGRLRECPILDYKFVMLSHNTVRG
                     AAGGAILNAELFIAKGGLEKLR"
     gene            complement(1081..2460)
                     /locus_tag="PLO58_05820"
     CDS             complement(1081..2460)
                     /locus_tag="PLO58_05820"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQO74341.1"
                     /translation="MKVLKFGGASLKDKNAYTETIRILAEDKEPKVVVLSAIYGMTDW
                     LETTIREARANEQSIENTTTKIRAWHAKILTDLVADPTMISDCMNELEERIQKLERLL
                     YGLAYTEEMTPQLYALIMSFGERLSVILSATIFKAHGIPAQYLESDKIGIVTDDSFTS
                     ATALLPIVRKNLPSHVYPLLEKGITPLITGFFGVNKNGKVTLFGRNSSDYSAAVIAQG
                     LKADRLEIWKDVDGFMTADPKIVPTATLIDKLSYYEAAELSYFGAKILHPRTVEPLIH
                     DSIPLVIRNIYHPEIEGTEICAKEILNTAIIKSVTYNKNISVLRIKGAGVGYKPGVIS
                     EIGSRLAACGVNIYSIITSQTCINLLIDRKDSKVGYKALQVLKDGLIDSIEHCDDEVV
                     VAAVGAGLKQTPGLAAKIFSAIAGQNINIEMISLGASDVATYFIVKEPCLVPAVNALH
                     TAFFTNSIN"
     gene            complement(2477..3190)
                     /locus_tag="PLO58_05825"
     CDS             complement(2477..3190)
                     /locus_tag="PLO58_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HQO74342.1"
                     /translation="MNQTVTFRYKNTIARITGEPELVKIGEEEIIRCYTDIENYISND
                     PIFKATLKPYKVPNDASKIIREMATAGAICGVGPMAAVAGAIAKYAVLAMQKAGATFA
                     IVDNGGDIAILNDRPVVVGIYSGPAVIHDIGLRIPVHSTLTSVCTSSGVIGHALSFGK
                     SHASVILADDPVLADAAATALGNRIREKDKFEIENAMNEVLKFGVAGCIVIIEDYVGF
                     AGEIPEICRVEMSESMIAK"
     gene            complement(3204..4550)
                     /locus_tag="PLO58_05830"
     CDS             complement(3204..4550)
                     /locus_tag="PLO58_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HQO74343.1"
                     /translation="MKIVKSYAEINQKIKNRQAVVMTAEEIKSFIREQGIKSVLEKVD
                     VVTTGTFGAMCSSGAFLNFGHTDPPLKMEKVKINDVPAYGGLAAVDAYIGATERSQKS
                     NGHYGGGHVIEDMVAGKNLVLEAEGKPTDCYPLDSIVTEFTLKDLNQAVLLNPRNAYQ
                     RYNAAANSTDRIIYTYMGRLLPRLGNVTYSGAGELSPLNNDPNFLAIGLGTRIFLGGG
                     VGYVIDSGSQHSPETGFANLMVKGDLKLMRPEYLRGAYVYGYGCTLFVGIGVPIPLLN
                     EEIARSVAITDDELRVNIVDYGIPRLKRPILRTGVTYRELKSGQVEIEGKPVPTGSLS
                     SLFMARKIANELKNWILNGNFLLTEAIEPIPKIGKAKQLAVKPPKKPQAISTTNQKHS
                     NGIQIDHSRCILCELCIAQCPEKAIYLNENNQITIDQAKCNLCRICTKVCSVGCLE"
     gene            complement(4704..>5018)
                     /locus_tag="PLO58_05835"
     CDS             complement(4704..>5018)
                     /locus_tag="PLO58_05835"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQO74344.1"
                     /translation="ERIRQIDEISTAFIYCVAYTGVTGQDNRPTPETIAFFNHLKQTL
                     WHPWLIGFGVKSHEDFQTYTHYADGVIIGSAFINLLENTPENNRLAACRTFVQNIINS
                     PS"
     assembly_gap    5019..5118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5119..5324)
                     /locus_tag="PLO58_05840"
     CDS             complement(<5119..5324)
                     /locus_tag="PLO58_05840"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQO74345.1"
                     /translation="MSTRLNQILKSKKEKQEKCLSIFLTAGFPEKNCTPEIILTLAAS
                     GVDFIELGIPFSDPIADGPVIQKAS"
     gene            complement(5334..6509)
                     /gene="trpB"
                     /locus_tag="PLO58_05845"
     CDS             complement(5334..6509)
                     /gene="trpB"
                     /locus_tag="PLO58_05845"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQO74346.1"
                     /translation="MEKGYFGEYGGQYVPEILRPALVELEEIYLALKDDPDFQKRLNI
                     ELHDYAGRPTPLYFAENLTRHYGQAKIYLKREDLNHTGAHKINNAIGQILLAQAMHKK
                     RIIAETGAGQHGVAVATVAARAGLECCIYMGAEDIQRQYPNVRRMQLLGAEVRPVHTG
                     TATLKDATNEALRDWLANVKTTHYIIGSVVGPHPFPTIVRDFQKIIGEETREQIVEKT
                     GRLPDYILACVGGGSNAMGIFYPFISDTAVNLIGVEAAGEGLASGKHSATLSCGSLGI
                     FHGMKSFVLQNNDGQIQLPYSLSAGLDYPGVGPEHCHLKDSGRVKYYAVTDTEALAAV
                     ELLCRLEGIIPALESAHALAYLEYLLPQTHSRESVVVNLSGRGDKDLETYLKYLERR"
     gene            complement(6514..7143)
                     /locus_tag="PLO58_05850"
     CDS             complement(6514..7143)
                     /locus_tag="PLO58_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HQO74347.1"
                     /translation="MPVKLKICGITSLEDALLAAQLGADWLGFNFYPASPRYIAPELA
                     AEIIKVLPRTVESVGVLVKPFLEEARKIITITGVQRLQIYEPQDFNDYSQLGVPVIAC
                     YRCKNGKITELNYASADMVLLDNYAESIFGGTGQAFNWRWIPTELERKKLVLAGGIDT
                     TNILEALEMIGPAVIDVASGSESKPGHKDPEKIKTLVSQIRIFNQRKDN"
     gene            complement(7133..7930)
                     /gene="trpC"
                     /locus_tag="PLO58_05855"
     CDS             complement(7133..7930)
                     /gene="trpC"
                     /locus_tag="PLO58_05855"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQO74348.1"
                     /translation="MNWLDTIVAHKKQELIDAKKAVPLEFYQAQIGDNCSNHSLLRTI
                     KQQGGFHFIGEVKRASPSRGIINSDFDPLQQAKCYAKGGASAISILTDSQFFMGNLAD
                     LQVIREQIDLPLLRKDFIVDPYQIYESRYYQANIVLLIARILSPKQITQFCELATKLA
                     LEVLLELATENDLVKIPSNHSNLILAINNRDLANFEVDLNNSFRLKPLLPDDIPVISA
                     SGISNTDECVRLFKNGFAGALIGESLMRDREPDSLLKKFRSAIANAG"
     gene            complement(7941..8966)
                     /gene="trpD"
                     /locus_tag="PLO58_05860"
     CDS             complement(7941..8966)
                     /gene="trpD"
                     /locus_tag="PLO58_05860"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQO74349.1"
                     /translation="MIKEYLEKISQCEDLTRQEAATALQMLIDDQLTATEIGALLFGL
                     RTKGESVEEILGFVDTMQANMVAVAIDDPDAIDVCGTGGDQSHSFNVSTTAAFVVAAG
                     GVTVAKHGNRSVSSKAGSADVLEKLGARIDLSPENTRRCLNEIGISFLFAPMYHPAMK
                     AVAPHRRNLGIRTIFNMLGPLINPANLKRQLIGTYNLETAAKTAAVLRQRCYKKACVV
                     HSEEGFDEASPFAGNRVFEVDIARPDIYEYFYRAEKCSQNLNSYQLVQGGDAEKNARI
                     TLEILNGRRGYPREMTALNAAFGLYVAGKVDNIPDGIFLAEELIDSGKALQKLNQFIE
                     MSSKLAN"
     gene            complement(8968..9564)
                     /locus_tag="PLO58_05865"
     CDS             complement(8968..9564)
                     /locus_tag="PLO58_05865"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQO74350.1"
                     /translation="MILFIDNYDSFTYNLVDYIGQLHNDVLVVRNDQINVAEIENLKP
                     DGIVISPGPGRPEQAGFSCQIIQTYCHKIPILGVCLGLQAIAQIFGANIVRAPFPVHG
                     KASIVEHNGQGIFQGMTSPMTAGRYHSLIIERESLPECFTITAESSDHLIMAIAHRKF
                     PLFGVQFHPESVLTLNGMQILRNWVKIADKFKNNTEEV"
     gene            complement(9545..11023)
                     /gene="trpE"
                     /locus_tag="PLO58_05870"
     CDS             complement(9545..11023)
                     /gene="trpE"
                     /locus_tag="PLO58_05870"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HQO74351.1"
                     /translation="MNYEDFEKLARPGLIIPVYRKFNADFITPVIAYLKLREAGKHSF
                     LLESVIKGEQLGRYSFLSQEPYLILKCRDDKTISIADHRSKIHRENFFTILQQQLKDY
                     ELLKIENLPRFTCGAVGYIGYEMAGQIEKLPKPKRDVIGSDDALLGFYNQLVAFDHLK
                     NEVILIANVFIDNDSDVRQLYLEAQNRLDCLQARLNRSYEGELRFTADLASEEANFTK
                     KDFMTAVEQAKEYILAGDIFQAVISQRFSMAYSGDVFQVYRALRNINPSPYMFFLDFL
                     DYQVIGSSPEPLIRAQDNELEIIPIAGTRQRGATSAEDDRLEAELLNDPKELAEHVML
                     VDLARNDLGRVATFGTVKVNDFKTIQKYSHVMHIISQVHGHLRDGLTVVDAFKSAFPA
                     GTVSGAPKIRAMEIINELEPEKRGIYSGAIGYFNYGGNLDLCIGIRTIVAGRKRLYYQ
                     SGAGIVADSQPENEYQETLNKCQALRQAIEQASGGINDFIHR"
     gene            complement(11332..13092)
                     /locus_tag="PLO58_05875"
     CDS             complement(11332..13092)
                     /locus_tag="PLO58_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HQO74352.1"
                     /translation="MSKFQSKIFDNKIVIGVRDWVAEDVEELLQSEIFKELLNRFLDR
                     LEKKQSPLLDIFGHYPIDDNDRRILYETLCYLTKLESKLVPNIVSDSQRLFADRHLLN
                     EFVEQLYNYWRSFDRFILCDSTGDNFDKRPYRTFNLTVERLTHLVRQAYRDIQENITA
                     QHPRVYRQVHAGAEVATIAVESHSSRFKAEYSQLGSISVIRQVLIYPPLILEPPMNKR
                     KGEFIEVTENPIKSVQFNTQEWLCYPAKVGSLIVYVYFHESFYELGFALCNLFELASD
                     RDLRRKPDGIFLYGVPEENLRHLADFPTVFYEDKANDLFVGAVPRGNEFGYFGYLKKM
                     ILTLHNLRVMQKGHLPFHGAFVQILLKNGTQANVLMVGDTGAGKSETLEALRCLGDEY
                     IKDLTIIADDMGSLVIQDQVVKAYGTEIGAFLRLDDLQPGYAFGQLDRAIIMNASKTN
                     ARIVLPVTSYENIIKGVPVDYIFYANNYENVDADHPVIEKFTTPDEALYVFREGTVMS
                     KGTTTTTGLVHSYFANIFGPAQRKAIHDKIARRYFVHFFEHKVFVGQLRTRLGVAGFE
                     IEGPKIAAEELLKILLTPSN"
     gene            complement(13258..14106)
                     /gene="thyX"
                     /locus_tag="PLO58_05880"
     CDS             complement(13258..14106)
                     /gene="thyX"
                     /locus_tag="PLO58_05880"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HQO74353.1"
                     /translation="MSHVVVPEAEALLDKEIKVLDHGFIRLVDYMGGDSRIVQSARVS
                     YGKGTKSVREDEALIDYLMRHQHTSPFEQVVLTFHCKMPIFIARQWIRHRAARVNEIS
                     GRYSVMEDEFYLPEETAIQYQNKDNRQGRDPQEVPVHLRQKVLNILAKGQQVAYDDYQ
                     KMLADDIARELARINLPLSLYTQWYWQIDLHNLFHFLELRMDEHAQWEIRQYANVIAD
                     ITRAVAPLAFRAFETHVVNAVRFSGSEIQALAANLRGQEHDLKGSRKIEYEAKLRKII
                     GNPGGE"
     gene            14240..>15708
                     /gene="lepA"
                     /locus_tag="PLO58_05885"
     CDS             14240..>15708
                     /gene="lepA"
                     /locus_tag="PLO58_05885"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQO74354.1"
                     /translation="MELNIRNFCIIAHIDHGKSTLADRMLEFTGTISRREMKDQVLDN
                     MDLERERGITIKSHPIQMNYHAPDGKNYILNLIDTPGHVDFSYEVSRSLAACEGALLL
                     VDASQGVEAQTVSNAYLAIENDLAIIPVINKIDLPGAMIPEAKRQIIDLIGCDESEII
                     LTSAKSGVGIEEIFEAIIKRIPPPKYEPDKPLRALIFDSVFDSFRGAIPYVRIFEGEV
                     RPGDWIRFYANNSTHEVSEVGIFKMQRQKTEKLSSGDVAYLVAGVKDISNIKVGDTIT
                     LKNNPASEPLPGYRVIKPVVFSGLYPVAAEDFEDLRSSLEKMKLNDSSLIYEPETSTA
                     LGFGFRCGYLGLLHMEIVQERLEREFNLNLITTMPNVEYQALLKTGELIEIDNPTKMP
                     AMGEIAEIREPYVRAEIIVPTTYLGNVLKLCQDKRGIYKTTKYLDITKVQIQYELPLA
                     EIVFDFYDKLKSVSRGYASFDYEPIGYRAGDLVKVDILIS"
BASE COUNT         4330 a         3646 c         3208 g         4424 t
ORIGIN      
        1 tacctaagtt tttccaaccc gcctttggca ataaaaagtt cggcattgag aatggctcca
       61 ccagctgcac cccgaaccgt attatgcgat aacatcacaa acttataatc gaggatggga
      121 cactcccgca gacgaccgag actaacggtc ataccgtttt ccagaagtcg atgtaatctc
      181 ggttgcggat aactgctttc ttcaaaatag caaagcggct tttccggtgc gcttggcagt
      241 ttcaaacgtt gcggttcacc cttgaattca cgccaggcgg cgatgatctc tccagcagtc
      301 ggttttcgtt ccaatttaac tgaaacgcat tccgtatgac catcaacgac ggcgacgcga
      361 ttgcaagccg cgctaatgat aaattgggct ggttcgatat gcccatcaag gtatttcccg
      421 agtatctttt ggggttcact ttctaatttc gcttcttcac cgctgataaa tgggataaca
      481 ttatccacaa tatccagact ggcgacgccc ggataaccag caccggaaat cgcttgcata
      541 gtaacaatat taacggccga aagcccaaat tgatcgagta aaggtttgag agctacaacc
      601 agaccggtcg cggcgcaatt cggattggtg ataatcgcac ctcgtccata tttctgagta
      661 gcgataattg ccaagtgatc ggaattgact tccggtacca gaatcggcac atcggggtca
      721 tagcgatgat tgcgtgaatt agaaattaca atatacccgg ccttagcaaa atcagtttca
      781 atttctcctg ccacggaagc atccagcccg gaaaaaacaa tgcgacaggg tagatttggc
      841 tcacaatttt ttactatcat cttcgcaacg ttgtctggta aaggcttagt cataaaccaa
      901 tgagcggcat cacgataagt tttgccagcc gagcgatctg aagccgctaa agcggcaatt
      961 tcaaaccagg ggtgttctgt caatagttcg atgaattttt gtccgacgct accggtggca
     1021 cccagaatac caactgggat tttggacttt aactgactgt tcgatttcaa tttttttcct
     1081 ttaattaata gaattggtaa aaaaagctgt atgcaaagca ttgactgccg gcactaaaca
     1141 aggctctttg acgataaaat aggtcgctac atccgaagcg cccaatgaaa tcatttcgat
     1201 gttaatattt tggccagcaa tagcactgaa aattttcgcg gctaaaccgg gtgtctgttt
     1261 caatcccgcg ccaactgccg ctacgacgac ttcatcatcg caatgctcga tcgaatcaat
     1321 caatccgtct ttcaaaactt ggagagcttt atagccgact ttagaatctt tgcggtcaat
     1381 caacaaatta atacaggttt gcgaggtaat aatcgagtaa atattaactc cacaggcggc
     1441 taagcgactg ccgatttcgg aaataacgcc cggtttataa ccaacaccag cgccttttat
     1501 ccgtaaaacc gaaatgtttt tattataagt aacactttta ataattgccg tattgagaat
     1561 ttctttagca caaatttcgg tgccttcaat ctccggatga taaatattac gaatcactaa
     1621 tggaatcgag tcgtgaatca acggctcgac tgtgcgcgga tgtaatattt tagcaccgaa
     1681 gtaggaaagc tcagccgctt cgtagtagga taatttatca attaaggtcg ccgtcggcac
     1741 aatctttgga tcggcggtca taaatccatc cacatctttc cagatttcta aacggtcggc
     1801 tttcaaacct tgggcaatga ccgccgcgct atagtcggaa ctatttcggc caaagagggt
     1861 aactttgccg tttttattaa ccccaaagaa tccggtaatc agcggcgtaa tgcctttttc
     1921 aagcaatgga tagacatgcg atggcaaatt tttacgaact attggcaaca atgcggtagc
     1981 actggtaaag gaatcgtcgg tgacaattcc aattttatca gactctaaat actgagccgg
     2041 aatgccatga gccttaaaaa tcgtcgccga caaaattacc gatagccgtt caccaaagct
     2101 cataatcaga gcgtaaagct gaggggtcat ttcttcggta taagccagac cataaagcag
     2161 gcgttcgagt ttctgaatcc gctcttctaa ttcattcata cagtccgata tcatagtcgg
     2221 atcggcaact aagtcggtta atattttagc atgccaggct ctgatcttag ttgtggtatt
     2281 ttcgatggat tgctcgttgg cgcgagcctc ccggattgta gtttccaacc aatcggtcat
     2341 gccataaatt gctgatagaa cgactacctt cggttcttta tcctcagcca aaatacggat
     2401 tgtctccgtg taggcgtttt tatctttcaa actggcacca ccgaatttca atactttcat
     2461 accgattcaa attccttcat ttggcaatca tactttcact catttcaacg cggcaaattt
     2521 ctgggatttc tcccgcaaat ccaacataat cttcaataat tacgatacat ccggctacac
     2581 cgaatttcag tacctcattc atcgcatttt caatttcaaa tttatctttt tcacggatgc
     2641 gatttcctaa agcagtcgcg gcggcgtccg ctaacaccgg atcgtcagcg agaatgacgc
     2701 tggcgtgcga cttcccgaag cttagtgcat gtccgatcac cccggatgaa gtacagacgc
     2761 tggttagcgt agaatgcacc ggaattcgca agcctatatc gtgaattaca gccggtccac
     2821 tgtagatccc aaccactact ggccggtcat ttagaattgc gatatcaccg ccgttatcaa
     2881 cgatagcaaa ggtggcaccg gctttttgca tcgccagaac tgcatattta gcaattgccc
     2941 cggctactgc cgccatcgga ccgactccgc aaatagctcc ggcagtcgcc atttcccgta
     3001 taattttact ggcgtcgttc ggaactttat agggcttcaa cgtcgcttta aaaattggat
     3061 cgttggagat atagttttct atgtctgtat aacaacggat aatttcttcc tcaccaattt
     3121 taaccaattc aggctcgccc gtaatgcggg caattgtatt tttatatcgg aaagttaccg
     3181 tttggttcat ctcttgcctt actttattcc agacagccaa ctgaacaaac tttagtgcag
     3241 atacgacaca gattgcattt agcttggtca atagttattt gattgttttc atttagataa
     3301 atagccttct ccggacactg ggcgatacac agttcacaaa ggatgcaacg agaatggtca
     3361 atttgaattc cgttggaatg tttctggttg gttgttgata ttgcctgtgg ttttttgggt
     3421 ggcttgacgg ctaattgttt ggcttttccg atcttgggaa ttggctcgat cgcttcggtc
     3481 aggagaaaat tgccgttcag aatccaattt ttcaattcat tagcaatttt gcgagccata
     3541 aacagactgg aaagcgaacc cgtaggaacc ggcttgccct caatttctac ttgaccggat
     3601 ttgagctccc ggtatgtaac tccggttctc aggattggac gtttcaatcg tggaattccg
     3661 taatccacaa tatttaccct gagttcatca tcggttatgg caaccgaacg agcgatttct
     3721 tcattcagaa gtggaatcgg cacgccaatc ccaacgaaca gagtacagcc atagccatag
     3781 acataagctc cgcgtaaata ttcgggacgc ataagtttca aatcgccctt aaccattaaa
     3841 ttggcaaaac ctgtttccgg actgtgctga ctgcccgaat caattacata tcctactccg
     3901 ccgcctagga aaatacgtgt tcccagaccg atcgccaaaa aattcgggtc attattgagc
     3961 ggcgacagtt cacccgcgcc ggagtaggtg acattaccga gccgtggcag taatcttccc
     4021 atataggtgt aaataatacg atcagtcgaa ttagccgcgg cattgtaacg ttgataagca
     4081 ttacgtggat tgagcagaac agcctgatta aggtctttga gcgtaaattc agttacaatg
     4141 gaatcaagcg gataacaatc agtaggtttg ccttcggctt ctaagacgag atttttgccg
     4201 gctaccatat cctctatgac atggcctccg ccgtaatgac cattcgattt ttgggaacgc
     4261 tcggtagcac cgatataagc atccaccgcc gctaaaccac cgtatgctgg aacgtcgtta
     4321 attttcactt tttccatttt taagggcggg tcggtatgac caaaattcaa aaatgctccg
     4381 gaagaacaca ttgccccaaa agtaccagtc gtgacgacat caactttctc caaaacactt
     4441 ttaataccct gttcccgaat aaagctcttt atctcctccg ccgtcatcac aactgcctga
     4501 cggtttttta tcttctggtt aatctcggcg tagcttttta caattttcat ataatccttt
     4561 cagccaaaaa agcgtaataa tataataaat tttacccaga ttattggcgg actactttaa
     4621 atatttatga atattttctt attattttgt ataatttatg tataaatata cagcataatt
     4681 ttctgctaag gtcatcaagt acatcatgat ggtgagttta ttatattttg gacaaacgtg
     4741 cgacaagcgg caaggcgatt attttcgggt gtattttcca gcaaattaat gaaggctgag
     4801 ccgataatca ccccgtcggc atagtgagtg taagtttgga aatcctcatg acttttaacg
     4861 ccaaaaccga tcagccaagg atgccagaga gtctgcttta aatgattgaa aaatgcgatt
     4921 gtctcgggag ttggtcggtt atcctggccg gtaacgccgg tgtaagccac acagtaaata
     4981 aaagcggtac tgatttcgtc aatttgccga atgcgctcnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnga agctttctga ataaccggac cgtcggcaat cggatcggaa
     5161 aacggaattc ccaactcgat aaagtccacc ccgcttgcgg ccagggttaa aattatctcc
     5221 ggagtacaat ttttctccgg aaaaccggct gtgagaaaaa tcgagagaca tttttcctgt
     5281 ttttcttttt tactttttag aatttgattt aatctggtgc tcattggatt tccttaccgc
     5341 ctttccaaat atttgagata tgtttccaga tctttatcgc ctcgaccgct caggttcacc
     5401 actacggatt cccgactatg agtctgtggc aaaagatatt caagataagc gagagcatgc
     5461 gcggattcaa gcgccggtat gatgccttcc agtcggcata aaagctctac cgcggctaaa
     5521 gcttcagtat cagttacagc ataatacttg actcgcccac tatccttaag atgacaatgc
     5581 tctggaccga cgcccggata gtccaatccg gctgagagcg aatagggcaa ttgaatctgg
     5641 ccgtcattat tttgaagtac gaaggatttc attccatgaa aaataccgag tgagccacaa
     5701 ctcagtgtcg ccgaatgttt gccactggca agtccttctc cagccgcttc tactccgatt
     5761 aaattgacag cggtatcaga aataaacgga taaaaaatac ccatggcgtt actcccgccg
     5821 ccaacacaag ccagaatgta gtccggcaaa cgaccggttt tctcgacaat ttgttcgcgt
     5881 gtttcctcgc caataatttt ctgaaagtca cgcacaatcg tgggaaacgg atgtggtccg
     5941 actaccgaac cgattatata atgagtagtt tttacattag ccagccaatc ccgcaaggct
     6001 tcgttcgtag catctttcaa agtagctgtt ccagtatgaa ccggacggac ttcagccccc
     6061 agaagttgca tacgtcgcac attgggatat tgacgttgaa tatcttccgc tcccatataa
     6121 atgcaacatt ccagaccggc tcgcgccgcc acggtcgcaa cggcaacgcc atgctgaccg
     6181 gcgccagtct cggcgatgat gcgcttctta tgcattgctt gcgccagaag aatctgccca
     6241 atggcattgt taattttatg agcgccggta tgattcaaat cctcacgttt caaataaatt
     6301 ttagcctgac cataatggcg ggtcaggttt tcggcaaaat ataaaggcgt tggacgaccg
     6361 gcgtaatcgt gcaactcaat gtttaaccgt ttttgaaaat cgggatcatc tttaagcgct
     6421 aaatatattt cttccaattc gaccaatgcc ggacgcagga tttccggaac atactgtccg
     6481 ccatattcgc caaaatatcc tttttccatt gaattaattg tcctttctct gattaaatat
     6541 tctaatttga ctaactaatg tcttgatctt ttccggatct ttgtgaccgg gctttgattc
     6601 acttccgctg gctacgtcaa ttacggctgg accaatcatt tctaaagctt ccaagatgtt
     6661 agtggtatct atcccgcctg ccagcaccag tttctttcgt tccagttcgg tcggaatcca
     6721 acgccaatta aaggcttggc cggttccccc aaaaatactt tccgcataat tatccagcaa
     6781 aaccatatca gccgacgcat aatttaattc ggttatttta ccatttttac aacggtaaca
     6841 agctattacc ggcactccta attgggaata gtcattgaaa tcctgcggct cgtaaatctg
     6901 caagcgctga actccggtga ttgtgataat tttgcgagct tcttccagaa aaggttttac
     6961 caatacaccg acactctcaa ccgtcctcgg aagcacctta ataatttcgg cggcgagttc
     7021 cggtgcaatg tagcgcgggc ttgcgggata aaaattaaat cccagccaat cggcgccaag
     7081 ttgagcggca agtaaagcat cctccagcga ggtaatcccg cagattttca atttaaccgg
     7141 cattagcgat tgcactcctg aacttcttca ataaactatc cggttcgcga tcacgcatta
     7201 atgattcgcc aattaatgca ccggcaaagc cgtttttaaa aagtcgtaca cactcgtcgg
     7261 tgttacttat tccgctggca ctgatgaccg gtatatcatc aggtaaaagg ggcttcagac
     7321 ggaaggaatt attcaaatcc acttcaaaat ttgccagatc acggttgttg atcgccagaa
     7381 ttaaattgga atgattagac ggaattttta ctaaatcgtt ctcggttgcc aattccaata
     7441 aaacttccag agccagtttc gttgctaact cgcaaaattg ggtaatttgc tttggcgata
     7501 gaatccgagc aatcagcaag acaatattgg cttgataata acgagattca taaatctgat
     7561 aagggtcaac gataaaatcc ttacgcagaa gcggcaagtc aatctgttct cgaataactt
     7621 gcaaatcagc caaattaccc ataaagaatt gcgagtccgt cagaatggaa atagcacttg
     7681 ctcccccttt cgcatagcat ttcgcctgct ggagcggatc gaaatcagaa ttgataattc
     7741 ctcgagacgg tgaagccctt ttaacttcgc cgatgaaatg aaaaccccct tgctgtttta
     7801 tcgttctaag caagctatgg ttggagcaat tgtctccaat ttgagcttga taaaattcca
     7861 gcgggacggc tttcttagcg tctattaatt cctgcttttt atgagcgacg atagtatcaa
     7921 gccaattcat atctcttcgt ttaattagcg agtttgctac tcatttcgat aaactgattc
     7981 agtttctgca aggctttgcc gctgtcgata agttcttcgg caaggaatat tccatcagga
     8041 atattgtcga ctttaccggc gacataaaga ccaaaagccg cattcaaagc cgtcatttca
     8101 cgcggatagc ctcgccttcc atttaatatt tccaaagtta tccgcgcgtt tttctcggca
     8161 tccccgcctt gcaccagctg ataactgttc aggttttggg aacatttttc cgctcggtaa
     8221 aaatattcgt aaatatccgg tcgagcaatg tcaacttcaa atacccgatt accggcgaac
     8281 ggcgatgctt cgtcaaagcc ttcctcgcta tgcaccacac atgctttttt ataacatcgt
     8341 tgtctcaaaa cagccgctgt tttggcggcg gtttccagat tgtatgtccc aatcaattgc
     8401 cgcttcaaat tggctgggtt tatcaatggt ccaagcatat tgaaaatcgt gcgaattcct
     8461 aagtttcgac ggtgcggagc tacggctttc attgccggat gatacatcgg cgcaaataaa
     8521 aaactgatcc cgatttcatt cagacatctg cgagtatttt ccggagaaag atcaatccgg
     8581 gcgcccaact tttccagaac atccgcggag ccggctttgc tggagaccga gcgattacca
     8641 tgcttggcaa cggtaactcc accagccgcc accacaaaag ccgcggtcgt tgaaacatta
     8701 aatgaatgac tctgatcacc tcctgtgcca caaacatcaa tggcatctgg atcatcaatt
     8761 gcgaccgcca ccatattggc ttgcatcgtg tcaacgaatc ccagaatttc ctctaccgat
     8821 tcgcctttcg tgcgcaatcc aaataataga gcaccgattt cggtggcagt caactgatca
     8881 tcaatcaaca tttgcaaagc cgtagcggct tcctggcgag taaggtcttc acattgactt
     8941 attttctcaa gatattcttt gatcatatta cacctcttct gtattatttt taaatttgtc
     9001 cgcaatcttt acccagttgc gtagtatttg cattccattt agggtcaata ccgattcggg
     9061 atgaaattgg actccgaaga ggggaaattt gcggtgagca attgccatta tgagatgatc
     9121 actactttcg gctgtgatcg taaagcattc cggcaaagat tcacgctcaa ttatcagtga
     9181 gtgatagcga ccggccgtca tgggcgaagt cattccttga aaaattccct gaccgttatg
     9241 ttcgacaatc gaagccttgc catgcactgg aaagggtgcc cgaacgatat tagccccgaa
     9301 tatctgagcg atcgcctgta atccgaggca tactcccaga ataggaattt tatggcaata
     9361 agtttgaatt atctgacagg aaaatccggc ttgttccggc cgaccgggtc ccggagaaat
     9421 gacaatccca tccggtttta agttttcgat ctccgcaaca tttatttggt catttctcac
     9481 gactagcaca tcgttgtgca gttgaccaat gtaatccaca aggttataag tgaaagaatc
     9541 atagttatct atgaataaaa tcattgattc ctccactagc ttgttctatt gcctgtcgga
     9601 gagcctggca tttattgaga gtttcttggt attcattttc gggctggcta tccgcgacga
     9661 ttcccgctcc actctggtaa tatagtcgtt tccgaccggc gacgatggtg cggatgccga
     9721 tacacaaatc cagattgcca ccataattga aataaccgat agctccggag taaattccgc
     9781 gcttttccgg ttcaagttca ttaataattt ccattgcccg aattttaggc gcgccggaaa
     9841 ccgttccggc tggaaaagcc gacttaaagg catctaccac agtcaaacca tcgcgcaaat
     9901 gaccgtgcac ttgagaaatg atgtgcataa cgtgcgaata tttttgaata gttttaaaat
     9961 cattgacttt aacagtgccg aatgtcgcta cccggccgag gtcattacgg gctaaatcca
    10021 ccagcataac atgttcagcc agctctttgg gatcattcag tagttcagcc tccaggcgat
    10081 catcttcagc ggaagttgcc cctcgctgac gagttccggc gattggaatg atttccagct
    10141 cattatcctg agcccggatc aaaggttccg gtgaggaacc gattacctga taatccaaaa
    10201 agtccagaaa aaacatatat ggcgatggat taatgttgcg caatgcccga tagacttgaa
    10261 aaacatcgcc ggaataagcc atcgaaaaac gctgagaaat cacggcttga aaaatatcgc
    10321 ctgccagaat atattcctta gcctgctcaa cggcagtcat aaaatccttt ttggtaaaat
    10381 tggcttcctc gctcgccaga tcagccgtaa atctcagttc accttcatag ctgcgattta
    10441 atcttgcttg cagacaatcc agacggtttt gggcttcgag atacaactgt cgaacatcac
    10501 tgtcattgtc aataaaaaca ttagcgatca gaatgacttc atttttcaaa tgatcgaaag
    10561 ccaccagctg attgtaaaag cccagcagag cgtcgtcgct tccgattaca tcgcgcttgg
    10621 gcttcggcaa tttttcgatc tgacctgcca tttcgtaacc gatatatccg accgctccac
    10681 aggtaaaacg cggcaagttt tcaatcttca gaagttcata atcttttaac tgctgttgca
    10741 gaatagtgaa gaaattttct cggtgaattt tacttctatg gtcggcgatt gaaatcgtct
    10801 tgtcatcgcg gcatttcaaa attaaataag gctcctgact caggaatgaa taacgcccca
    10861 actgttcgcc tttaatgaca ctttccagta aaaaggaatg tttcccagct tcgcgtaatt
    10921 tcaggtaagc aatcaccggc gtaataaaat cggcattgaa tttcctataa acgggaataa
    10981 tcaaacccgg cctggctaat ttttcaaaat cctcataatt catatttctc tcgttttgct
    11041 ttttccttat aaaatgaaaa acccgccggt catttctcag gcgggtttca taatttttat
    11101 atatttttat ttcttattta ctcatattta aacctgagga aatgacgctt atccatcatt
    11161 tgccaccacc agtttatgtt catttgcatt cgcatactat ctataattct ctaaaaatac
    11221 gtggcaattt taaacaaatt ctcttttgta gtcaagaagt ttttttgatt ctattttaat
    11281 tttcgatcta acttcaatct attaagtatt tggcttaaaa ttttaaaact attaattgga
    11341 tggggttaac aaaattttta gcaactcctc agcggcaatt ttcggaccct caatctcaaa
    11401 tcccgcaacc ccaagccgcg ttcgcaattg cccaacgaaa accttatgtt caaaaaaatg
    11461 aacaaagtaa cgccgcgcaa ttttatcatg aatggcttta cgctgagccg gtccaaaaat
    11521 attggcgaaa tagctatgga ccaatccggt ggtagtagtt gtccctttac tcataaccgt
    11581 accttcccgg aaaacgtata aagcctcatc gggagtggta aatttttcaa ttacgggatg
    11641 gtcggcatca acattttcat aattattagc atagaaaata taatcaaccg gtacgccctt
    11701 aatgatattt tcataagaag taaccggcag gacaatgcga gcgttagttt tgctggcatt
    11761 cataatgata gcccggtcta gctgcccaaa agcatagccg ggttgcaaat catccagtcg
    11821 caggaaagct ccgatttcag tgccataagc ttttacaact tgatcttgaa taaccagcga
    11881 gcccatatca tcggcaatga tggtcaagtc tttaatatat tcatcaccga gacagcgtaa
    11941 agcctctaat gtttcggatt taccagcacc ggtatcgccg accatcagta cattagcctg
    12001 agttccattt ttcagcaaaa tttgcacgaa agctccatgg aaaggcaaat gccccttttg
    12061 catcaccctc aagttatgta gagtcaagat cattttttta agatagccaa aataaccaaa
    12121 ttcattcccg cgcggtaccg cgccgacgaa taggtcgttg gctttatctt cgtaaaaaac
    12181 tgtcggaaag tcggctaaat ggcgcaggtt ttcttccggc actccataca aaaaaatacc
    12241 atccggtttc cgacgaagat cccgatcact cgccagctcg aaaagattac aaagggcgaa
    12301 tcccaactcg taaaacgact cgtgaaaata gacataaaca atcagcgagc caactttagc
    12361 cggatagcag agccattcct gagtattgaa ttgtaccgac ttgatcgggt tttcggtaac
    12421 ttcaataaat tcacctttgc gcttgttcat tggtggttcc agaatcagcg gtggataaat
    12481 caagacttgc cggattaccg agatagagcc taactggcta tactcggctt taaaacgcga
    12541 actatgagat tcgaccgcaa ttgtggctac ttctgccccg gcgtgcacct gtcggtaaac
    12601 tcgcggatgt tgggcggtaa tattttcctg aatatcgcga taagcctggc gaaccaaatg
    12661 ggtcaatcgt tcgacagtca aattaaaagt gcgatatggt cgtttgtcga aattatcgcc
    12721 ggttgaatca caaaggataa aacggtcgaa actgcgccag taattgtaca attgttcgac
    12781 gaattcattc agcaaatgcc ggtcggcgaa taatcgttgg ctatcagaaa caatattcgg
    12841 aaccagttta ctctccaatt tggtaagata acataatgtt tcataaagaa ttctacgatc
    12901 attgtcatct attggataat gtccgaaaat atccagcagg ggcgactgct tcttttccag
    12961 gcggtcgagg aaccgattca ataattcttt gaaaatttca ctctgcagga gttcctcaac
    13021 gtcctcggca acccagtctc gaacgcctat aacgatttta ttatcgaaaa ttttcgattg
    13081 aaatttactc ataacagcac agccttttct aaataaaaat aatcaagtta accggttaat
    13141 ttagtatcac tcgattattt tgcaaatgta ttccaattat tttaacctgc atcagtactt
    13201 gctaatccat tatttttatt attagaaacg acttgatttc gtaaacaatg gatattatta
    13261 ttccccgccc gggttaccga taattttacg cagtttggct tcgtattcga ttttacgact
    13321 acctttcaag tcgtgttcct gaccgcgcaa attcgcggct aaagcctgaa tttccgagcc
    13381 ggagaatcga acggcattga caacatgcgt ttcgaaagcc ctgaatgcca gcggtgcaac
    13441 tgcccgcgta atatcggcta ttacattagc gtattgtcgt atttcccact gggcgtgttc
    13501 atccatccgc aattccagaa agtggaaaag attatggagg tcaatctgcc agtaccattg
    13561 agtatataac gaaagcggca gatttatgcg cgccaattct cgcgcaatat cgtcagctaa
    13621 cattttttga tagtcgtcat aagccacttg ctggcctttg gctagaatgt tcagaacttt
    13681 ctgccttaga tgaacaggta cttcctgcgg atcgcgtcct tggcgattgt ctttattttg
    13741 atattgaata gcggtttcct cagggagata aaattcgtcc tccataacgc tataacgtcc
    13801 agaaatctca ttgacgcgtg ccgcgcgatg gcgaatccat tgtcgagcaa tgaaaattgg
    13861 cattttgcaa tgaaatgtta aaaccacctg ctcgaaaggc gaagtatgct ggtgacgcat
    13921 taagtaatca atgagcgcct cgtcttctcg caccgatttg gttcccttgc catatgaaac
    13981 ccgagcgctt tgcacaatcc gactgtcgcc tcccatatag tccactagac gaataaatcc
    14041 gtgatcgaga actttgattt ccttgtccag aagtgcttcg gcttccggaa ctaccacatg
    14101 cgacatttaa attctcctta ttctaaacct cggaaattta ggatttttat tattaccgaa
    14161 atccgaattt tttaaaatac attaaaaatt atattggaat ttttgttaat caggctgtat
    14221 ctgccgtaaa ttctccgacg tggaattgaa tattcgtaac ttttgcatta tcgctcacat
    14281 tgaccatggg aaatcaactc tggccgatcg gatgctcgaa tttaccggta ctatcagccg
    14341 acgtgaaatg aaggatcaag tcctggataa tatggacttg gaacgcgaac gcggtattac
    14401 catcaagagt catccgatcc agatgaatta tcacgccccc gatggtaaaa actatatttt
    14461 gaatttgatt gatactccgg gacacgtgga cttttcctat gaagtatcgc ggagtctggc
    14521 ggcttgtgaa ggcgctttgc ttttggtaga tgcctcccaa ggagttgaag ctcaaacagt
    14581 cagcaatgct tatctcgcca tcgaaaacga cctggctatt attccagtaa taaataagat
    14641 agatttgccg ggagcaatga ttccggaagc caaacgtcaa atcattgatt tgatcggttg
    14701 tgatgaaagc gagataatcc tgaccagcgc taagagcggt gttggcattg aagagatttt
    14761 cgaagcaatc attaagcgca ttccaccacc taaatacgaa ccggataagc cgttaagagc
    14821 cttgattttc gattcagttt ttgattcatt tcgcggcgcc atcccttatg tccggatttt
    14881 cgaaggtgag gtgcgtccag gtgattggat tcggttctat gcaaacaact ccacccacga
    14941 ggtttccgaa gtcggcatat tcaaaatgca gaggcaaaaa actgaaaaat taagttccgg
    15001 cgacgtcgct tatttggtag ctggagtcaa agatatttcg aatattaagg tcggcgacac
    15061 cattaccctg aaaaacaatc cggcgagtga acctctgccc ggctatcggg tcattaagcc
    15121 agtagttttt agcggcttgt atccggtagc ggctgaagat tttgaagatt tgcgttcatc
    15181 gctggagaag atgaaactaa atgactccag cttgatttac gaaccggaaa cttcaacggc
    15241 actcggtttt ggcttccgct gtggctactt aggactatta catatggaaa tcgttcagga
    15301 gcgtctggaa cgcgaattca atctgaattt gataaccact atgccgaatg tcgagtatca
    15361 ggctttactg aaaaccggcg aacttatcga aatagataat ccaaccaaaa tgccggcaat
    15421 gggcgagatt gccgaaatac gcgagccata cgtacgggct gaaattatcg taccaacaac
    15481 ctacttgggg aacgtcctga aactttgtca ggataaacgt ggtatttaca aaacaactaa
    15541 atacctcgac attacgaaag tgcaaattca gtatgaattg cccttagctg aaatcgtttt
    15601 cgatttttac gataaactca agtcagtatc gcgcggttat gcctccttcg attacgagcc
    15661 aattggatac cgagccggtg acctagtgaa agttgacatt ttaatttc
//
LOCUS       DAOMJU010000047        14330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_4162_length_14330_cov_20.262977, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000047 DAOMJU010000000
VERSION     DAOMJU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 14330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14330
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4162_length_14330_cov_20.262977"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..2163)
                     /gene="fusA"
                     /locus_tag="PLO58_05890"
     CDS             complement(67..2163)
                     /gene="fusA"
                     /locus_tag="PLO58_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQO74355.1"
                     /translation="MKSENLSKVRNIGIMAHIDAGKTTTTERILFYTGKVYKIGEVNE
                     GSATMDWMEQERERGITITSAATTCYWNQHRINIIDTPGHVDFTAEVERSLRVLDGVV
                     AIFCAVGGVEPQSETVWRQADKYHIPRIAFVNKMDRTGADFFNVLDMIEKRLKAQPVA
                     VTLPLGSGELFTGIIDLITQQAILYDDNSLGSQYYNIEIPKDLKEIATQYRQKLIEEV
                     ANFDDVVFAKYLNNEDITPQELQNGLRKGTLENKIVPVLCGSAFRNKGIQPLLDAIVN
                     YLPSPFDMPPIEGVNLKGTPIKRYPRPDEPLSALIFKIMSDPHVGRLSFVRIYSGVIS
                     KGDMVFNSTVQKKERISRLLLMHANKREDREMLEAGEIGAIVGLKYSYTGHTLCDPKN
                     PIILESMQFPEPVIKISIEPKSKADEENLINALNKLADEDPTFEISTDEETGQILIAG
                     MGELHLEILVERLIREFNVKAHIGKPQVSYRETVVSVAEGEGRFVRQTGNKSQFAVVR
                     LRIEPDSESNEVRIENRIVPGTLPKEFVPAIEKGIRNHVKSGVLAGYPLQKVRVIITG
                     AEYREEESTELAFEVAAAMAFENALHKAELALLEPIMALEVVVTDEYLGDVLSDLNTR
                     RANIINVNKRGDANVIDAEVPLREMFGYATDLRSISQGRAVFTMKFSQFDYCDKRVQR
                     EIIEKARGFVPEFLKN"
     gene            complement(2184..2651)
                     /gene="rpsG"
                     /locus_tag="PLO58_05895"
     CDS             complement(2184..2651)
                     /gene="rpsG"
                     /locus_tag="PLO58_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQO74356.1"
                     /translation="MSRRKKVISREVLPDPKYGDIMVARFINYLLRKGKKSVAEASFY
                     DAIAQIEAKHKGNGLDVFKKAINNVSPIMEVKSRRIGGATYQVPIEVRPERRFALASR
                     WLITYAKARPGKSMAEKLAAEILAAANGEGNAIKKREDTHKMAEANKAFAHFR"
     gene            complement(2667..3038)
                     /gene="rpsL"
                     /locus_tag="PLO58_05900"
     CDS             complement(2667..3038)
                     /gene="rpsL"
                     /locus_tag="PLO58_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQO74357.1"
                     /translation="MPTINQLIRKGREVVSKKEKAPALRGSPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGEGHNLQEHSMVLIEGGRVKDLPGVRYHIIRGTL
                     DSSGVNDRKNSRSRYGTKRPS"
     gene            complement(3114..>4460)
                     /locus_tag="PLO58_05905"
     CDS             complement(3114..>4460)
                     /locus_tag="PLO58_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQO74358.1"
                     /translation="VLNLVDDNNRITSTYNIPYGAHVFVNEGQLVKAGHTLFRWDVYM
                     DQILALYSGKVRFVDLIENVTYNIEYDESGHQTMTVIESKDRSLQPRIDIVAQENGKE
                     VVYPGYILPVRAMLVVRDGDEVVKGDILAKKPKEIGKTSDITGGLPRVAELFEARRPK
                     DPAVISEIDGKVTFGKTEKGVREIIVTGIDGTTKKYKIPYGRYVLVNQGDEVRAGERL
                     CEGPVAPQDILAVQDPRKVQEYLVNEIQEVYRLQGVRINDKHIEVIVRQMMQKVRIED
                     PGDTNLLEKDRVNRHELIEENNRIKDYVVIVNAGDSDWDEGDVVSKKEFAKFNRLLKE
                     EGKNPAKARPARPAQFTEMLLGITRASLNTESFISAASFQETTRVLTDAAVAGKTDYL
                     RGLKENVIMGRLIPAGTGLRKYKDISVIEPEEEIEEWKKLEEQKKEKENELAALQK"
     assembly_gap    4461..4560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4561..7241)
                     /gene="rpoC"
                     /locus_tag="PLO58_05910"
     CDS             complement(<4561..7241)
                     /gene="rpoC"
                     /locus_tag="PLO58_05910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQO74359.1"
                     /translation="MNYTKPSTFRSEFESITIHLSSPETILTRSHGEVLKPETINYRS
                     YKPEKDGLFCEKIFGPVKDWECHCGKYKRIRYKGIICDRCGVEVARKKVRRERMGHIT
                     LAVPVVHIWFLKSIPSKLYYLLDIPTKNLEEIVYYERYVVLNQGKSDLKYKTVITEEE
                     YFENMQAYGPKTELFDEEEAKNYFIAKTGGEAIRELLQKVDIESLVHSLKEELKIKKS
                     PTQKAEILKRLKVVMAFLNQEGKPINKPEWMVVTVLPVIPPDLRPLVPLEGGRFAASD
                     LNDLYRRVIIRNNRLKQLIEIKAPDVILRNEKRMLQEAVDSLFDNSRRRTEVRSGTRR
                     PLKSLSDMLRGKEGRFRQNLLGKRVDYSGRSVIVVGPDLRLNECGLPKDMAIELFKPH
                     IIHALLRRRLAKTPKAAKTMVERKDAEVYRVLEYVVKDYPVLLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQIHPLVCAAFNADFDGDQMAVHIPLTFEAQIECRLLALSSHNILHPAHG
                     HPIAIPSQDMVLGVYYITRQKEGARGAGLVFSSPEEAIYAYEIGEADLHAKVKIRIDG
                     KIIETTVGRALFNSLLPPGMGYYNELISKRSLEKIVGIVFQEAGNYETVKFLDNLKNI
                     GFKYATRSGVSISISDVLIPEEKEKILQESFERVEDIQRKFRKGILTEGERYNKVIDN
                     WTHTTNRLSEVMLEHMATDRQGFNPVYMMADSGARGSGEQIKQLAGMRGLMAKPQKSM
                     TGQVGEIIETPITSNFKEGLTVHEYFISTHGARKGLADTALKTADAGYLTRRLVDVAH
                     DVVISEEDCQTINGIEVTALKKEENIIESLADRIIGRVAQEDIYNPESEDEILVEVGE
                     EITEKTALKIQSAGIERVRIRSVLTCESLRGVCAKCYG"
     gene            complement(7269..11051)
                     /gene="rpoB"
                     /locus_tag="PLO58_05915"
     CDS             complement(7269..11051)
                     /gene="rpoB"
                     /locus_tag="PLO58_05915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQO74360.1"
                     /translation="MSNTLPLEKERKSFAKVKIVQKMPNLLEVQLKSFDEFLQKDTMP
                     LERKNIGLESAFRNVFPVEDTHGNYLLEYKYYTIGEPRYTIKECLERGVSYTVPLKVR
                     LVLHISEEGISKGEYQAGIEQDIFFGNVPFMTEKGTFIINGAERAIVSQLQRSPGVFF
                     DETEHTNGMKIYSARVIPFRGSWIDFVIDTRDILSAIIDRRRKFPATILLRAFGYPTN
                     EDIVNLFGIGKRFSLDGDSKELLGKMLAENVIDPETGEVISEIKEEIVVDKDFLKKCK
                     QHGITSLLVFTTDDEIERNIFLNTFRKDTQTNDEESALYFLYRSLRTGEPPNLDIAKK
                     FVERLFFSPKKYDLGQVGRYRINKKFNLKVPLEECVLTRDDIVEIMKRIVDMKKGNLG
                     PDDIDHLGNRRVRNVGEQLTNQFNLAFTRMARTIRERMNLRESENLTPQDLINSRIIT
                     SVINSFFGTSQLSQFMDQTNPLAEITHKRRVSSLGPGGLSRERAGFEVRDVHYTHYGR
                     LCPIETPEGPNIGLIASLATYAYVNDLGFIETPYRRVEKVNGIATVTDKVEYLSADDE
                     DREIIAQANEPLDEQGHFINSRVRARVRGEFPIVTPDEVKYMDVSPEQIVSVSAALIP
                     FLEHDDANRALMGSNMQRQSVPLLKPERPIVGTGLEGVVARDSQSVVLAPVNGIVEEV
                     TADHIVIRTDTENELYLDENQGKVRIELAKFLRTNQDTCINQKPLIKEGDRVKAGDAI
                     ADGCATNDGELALGRNVLVAFMPWRGYNFEDAIILNERLVYDDVFTSIHIKEFELEVR
                     DTKRGNEELTREIPNVSEEATKNLDANGIIRVGAEVEPNDILIGKVTPKGETDPTPEE
                     KLLRAIFGEKASDVKDASKRADPGIRGVAVDTKLFTRKSKVSRMEEKRQIDEETKRSV
                     QKHAYLKGRRDLLLKNILKGATSATIKNLTNGKVAIKSGIQFNDEILAQLDFDLLSLE
                     TPWTNDPEINEKVEKIVKSYFILSQRIDEELEQTIYKIKVGDDLQPGVLQLAKVYIAS
                     KRKVQVGDKMAGRHGNKGVVSIIVPKEDMPFLEDGTPVDIILNPMGVPSRMNLGQLYE
                     TMLGWAGRELGVYYETPVFNGASEQEVQEELRKAGLPEIGKVSLIDGQTGEQFDNPVT
                     VGYIYMMKLSHLVEDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAA
                     YTLQEILTVKSDDVDGRSKVYNAIVKGENFSDFGVPESFNVLLKELQGLCIEMDIN"
     gene            complement(11157..11549)
                     /gene="rplL"
                     /locus_tag="PLO58_05920"
     CDS             complement(11157..11549)
                     /gene="rplL"
                     /locus_tag="PLO58_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQO74361.1"
                     /translation="MANITRADVLEYLEKANMLEISELIKDIEKKFDVKAAAPVAVAA
                     TVDQPGAPAEAEAVEEQTEFNVILKEVGPNKINVIKVVRAATNLGLKEAKDLVDGAPS
                     TVKEGISKEEAAELKKQLEEAGAKVEIK"
     gene            complement(11579..12106)
                     /gene="rplJ"
                     /locus_tag="PLO58_05925"
     CDS             complement(11579..12106)
                     /gene="rplJ"
                     /locus_tag="PLO58_05925"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQO74362.1"
                     /translation="MPNQKNIQSLETISEKFQKARGIYFTNYKGLNVKEITDLRKELY
                     AADVEYKVVKKTLTKMAAKNSGLDSIEPLLDGQTALAISYTDPAIPGKVINTFIKKNK
                     LDKLVITGCIYDGVLFGADQVEKVINLPSRRELLASLLATLNAPMSQLTMVLRATLNQ
                     LVGTLNSLNEQKKQS"
     gene            complement(12121..12810)
                     /gene="rplA"
                     /locus_tag="PLO58_05930"
     CDS             complement(12121..12810)
                     /gene="rplA"
                     /locus_tag="PLO58_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQO74363.1"
                     /translation="MNISKRVEQNSKKVDRKKEYPLREALELLKNCNGAKFDETVEAH
                     VNLGVDPRHADQNIRGTVTYPHGTGRKVRVLVLTKSKVDEALQAGADYAGLEEYLEKI
                     QNGWTDVDVIIATPDVMGQVGKLGKILGPKGLMPNPKSGTVTNDVAAAIKEIKAGRVE
                     IRVDKTGIVHTIVGKKSFPIEHLEENVQTLISTLMRLRPPSVKGAYIQKVTLTTTMGP
                     GVRVDKSSIIA"
     gene            complement(12825..13250)
                     /gene="rplK"
                     /locus_tag="PLO58_05935"
     CDS             complement(12825..13250)
                     /gene="rplK"
                     /locus_tag="PLO58_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HQO74364.1"
                     /translation="MAKKVLATIKLQIEAGKANPSPPVGPALGQHSVNIVEFCKAFNA
                     RTQDQAGYIIPVVITVYSDRSFTFITKTPPASVLLLKAAKVEKGSGEPNKSKIGSVTM
                     DQVMEIAKIKMPDLNAHTVEKAAEMIKGTARSMGISINE"
     gene            complement(13258..13785)
                     /gene="nusG"
                     /locus_tag="PLO58_05940"
     CDS             complement(13258..13785)
                     /gene="nusG"
                     /locus_tag="PLO58_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HQO74365.1"
                     /translation="MEWYVLRTYSGKEKKVREAILIEAEHAGMKDIIKDVLVPSENIM
                     EMREGKKRVRNKVFFPGYILVNMDITKESQYLIENIPGVMNFVGAAGKPQPLKEDEVK
                     RVMGEVEKKDGREVLAVPFKMGDPVKVIDGPFIDFRGVVQEVNEDKRKLKVSVSIFGR
                     PTPVELDFLQVELEK"
     gene            complement(13796..13978)
                     /gene="secE"
                     /locus_tag="PLO58_05945"
     CDS             complement(13796..13978)
                     /gene="secE"
                     /locus_tag="PLO58_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HQO74366.1"
                     /translation="MIKRIREFFEGVVFEMKKVSWPTWSELKSSTAVVLIFSLILSVF
                     LFVVDLILSKIMNVIF"
     gene            complement(14027..14099)
                     /locus_tag="PLO58_05950"
     tRNA            complement(14027..14099)
                     /locus_tag="PLO58_05950"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14064..14066),aa:Trp,seq:cca)
     gene            complement(14111..14260)
                     /gene="rpmG"
                     /locus_tag="PLO58_05955"
     CDS             complement(14111..14260)
                     /gene="rpmG"
                     /locus_tag="PLO58_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQO74367.1"
                     /translation="MRELITLECTVCKRRNYSSDKNKKLQPNRVEYKKYCRWCKKHTV
                     HKETR"
BASE COUNT         3749 a         3320 c         2791 g         4370 t
ORIGIN      
        1 caatcgtacc aatgttgaga tgcggcttcg tccgcttaaa ttgttgcttt gccatatttc
       61 agctccttaa ttttttaaaa actcagggac aaaacccctg gctttttcaa taatttctct
      121 ttgaactctt ttatcacagt aatcgaattg ggaaaatttc atagtgaaaa ccgctcgccc
      181 ttgtgaaatt gacctcaggt cagtggcata accaaacatc tcgcgcaagg ggacttcagc
      241 gtcaattaca ttggcatcgc ctcttttatt cacattgata atattagctc gccgtgtatt
      301 cagatcacta agtacatcac cgagatattc atccgttacc accacttcca gcgccataat
      361 cggctcaagc aaagccagtt cggctttatg tagggcattc tcaaatgcca tagccgcggc
      421 tacttcaaaa gccagttcgg tggattcttc ttcacgatat tccgcaccag tgataataac
      481 acgtactttt tgtaatgggt atccggctag tactccgctt tttacatgat tgcgtatacc
      541 tttttcaata gccgggacaa attcttttgg taaagtaccc ggaacgattc ggttttcgat
      601 ccgaacttca ttgctttcgg aatccggctc aattctcaat cttacaactg caaattggct
      661 tttattcccg gtttgacgaa cgaatcggcc ttctccttct gcgacactca cgaccgtttc
      721 gcgatatgaa acctgcggct tgccgatgtg ggctttgaca ttaaactccc gaatcaggcg
      781 ttccaccaga atttcgagat gtaattctcc cataccggca atgagaatct ggccagtttc
      841 ttcatcggta gaaatctcaa aggttgggtc ttcgtctgcc aatttattca atgcattaat
      901 caaattttcc tcatcagcct tacttttcgg ttcgatagaa attttgataa cgggctctgg
      961 aaattgcatt gattcgagaa taatagggtt tttaggatca caaagagtat gcccggtata
     1021 actgtatttt aggccgacaa ttgccccgat ttcacccgct tccaacatct cacggtcttc
     1081 acgtttgtta gcatgcataa gaagtaaacg actgatgcgt tcttttttct gtacggtcga
     1141 gttaaaaacc atatcaccct tacttatgac gccgctataa attcggacaa aacttaatct
     1201 gccaacatgg ggatcggaca taattttaaa aatcagggca cttagcggtt catccggacg
     1261 tggatagcgt ttaattggcg tgccttttaa attgaccccc tcaataggag gcatatcaaa
     1321 aggtgatggt aaataattaa caattgcgtc cagcaaaggt tgaattcctt tatttcggaa
     1381 agccgaaccg catagtaccg gaacgatttt attctcgaga gtaccctttc ggagaccatt
     1441 ctgtagttcc tgcggagtaa tatcttcgtt atttaaatat ttggcaaaaa ctacgtcgtc
     1501 aaaattggcg acttcttcga tcaatttctg gcggtattgg gtagcaatct cttttaaatc
     1561 tttaggaatc tcaatattgt aatattgact tccgagagaa ttatcatcat ataatatagc
     1621 ctgctgagta atcaaatcta ttattccggt aaacagttca ccagacccca atggcaaggt
     1681 gactgctacc ggctgggctt tcagccgttt ctcaatcata tccaggacgt taaagaaatc
     1741 ggcgcctgtg cgatccattt tattgacaaa ggcaattcgt ggaatgtgat atttatcagc
     1801 ttgccgccag acagtttcac tctggggttc gacaccacca actgcacaga aaattgcaac
     1861 gacgccgtcc aagacccgca gggagcgctc cacctcagct gtgaaatcta catgtcctgg
     1921 tgtatctata atatttatgc gatgttgatt ccagtagcag gttgtggcgg cggaagtaat
     1981 ggtaataccg cgttcacgtt cctgctccat ccagtccata gtggctgaac cttcgttcac
     2041 ttcgccgatt ttgtaaactt ttccagtgta aaacagaatg cgctcagttg tagtagtctt
     2101 accggcatca atgtgagcca ttatgccgat atttctgact ttgctgagat tttctgattt
     2161 cactctactt tatcctcaat agatcatctg aaatgggcaa aagccttgtt ggcttcagcc
     2221 atcttgtgag tatcttcgcg ttttttgata gcattacctt caccattagc ggcggcgagt
     2281 atttccgcgg ctaacttttc tgccattgat tttcccggcc gagccttagc ataggtaatt
     2341 agccagcggg aagctagagc aaagcgtctt tcaggcctta cttcgatagg cacttgataa
     2401 gtagctccac cgattcgccg tgatttgacc tccataattg gtgaaacatt attaatagct
     2461 tttttaaaca catcaaggcc attaccttta tgcttggctt cgatttgagc aatggcgtca
     2521 taaaatgaag cctcggcgac ggatttttta ccttttctta aaaggtaatt aataaagcga
     2581 gctaccataa tgtcgccgta tttgggatcg ggtaggactt ctcgggatat aacttttttt
     2641 cgacgtgaca tacaatgttc tccctattag gacggacgtt ttgttccgta tctggagcga
     2701 ctgttcttgc gatcattgac accactggaa tccagggtac cgcggataat atgataacga
     2761 acacccggta aatccttaac acgaccgcct tcaattaaca ccatcgagtg ttcctggaga
     2821 ttatggccct cacctggaat ataagccgtt acttcgattc cattggttaa acgtacacgc
     2881 gctacttttc gcaaagccga atttggcttc ttgggagtag ttgtataaac gcgggtacaa
     2941 acgcctcttt tctgagggga gccgcgtaag gcgggtgctt tttctttctt actgactact
     3001 tcgcgaccct tgcgaattag ctggttaata gtcggcataa tttctccata ataaaagcct
     3061 gtaaaaatac gacacttaaa aattttaaac aataaatttt aacaaccaga tgtttatttt
     3121 tggagagcgg cgagctcatt ttctttctct ttcttttgtt cttctaattt tttccactct
     3181 tcgatttctt cttctggttc aatcacactg atatctttgt atttacgtaa accggttccg
     3241 gctggaatca agcgtcccat tatgacattt tctttcaaac cgcgtaggta atcggttttc
     3301 ccagctacgg cggcatcagt caaaactcgg gttgtttcct ggaaagaagc cgctgaaatg
     3361 aatgactcag tattaagtga ggcacgggtg ataccgagta acatttcggt aaactgggct
     3421 ggacgagccg ggcgagcctt agctggattc ttgccttctt ccttaagtaa tcggttgaat
     3481 ttggcgaatt cttttttgga aacgacatcg ccttcatccc aatcggaatc accggcattg
     3541 acgattacca cgtaatcctt gatacgattg ttttcttcaa taagttcatg gcgattaacg
     3601 cgatcttttt ccagcaaatt cgtatcgccg ggatcctcga tgcgaacctt ctgcatcatt
     3661 tgacgcacaa tgacttcgat atgtttatcg ttgattcgga caccctgtaa gcgatagact
     3721 tcctgaattt cgttaacgag atattcttga actttgcgtg gatcttgcac tgccagaata
     3781 tcctggggcg caactgggcc ttcgcataat cgctcaccag cccgaacctc atccccttga
     3841 ttgaccagaa cgtaacgacc atagggaatc ttatatttct tcgttgttcc gtcaataccg
     3901 gtaacgataa tttcgcgaac acctttctcg gttttaccga aagtgacttt accatcaatt
     3961 tcactgataa cagctggatc ttttggccgc cgggcttcaa acagttcagc tacccgtggc
     4021 agaccgccgg taatatcgct cgttttaccg atctctttcg gttttttggc taaaatgtct
     4081 cctttaacga cttcatcacc atctcgaacg actaacatag cacgcaccgg tagtatatac
     4141 ccgggataaa ccacttcttt tccattttcc tgagcaacga tatcaatacg tggttgtagc
     4201 gagcggtctt tggactcaat cacggtcata gtctggtgac cggattcatc atattcgata
     4261 ttataagtca cgttttcgat taaatctaca aaacgtactt tgccggaata gagggctaga
     4321 atctgatcca tataaacatc ccagcggaaa agggtatgac cggctttgac caattggcct
     4381 tcattgacaa aaacatgcgc accgtaggga atgttataag tagaagtaat ccggttgtta
     4441 tcatcaacca ggttcaagac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 ccataacact tggcacatac tccccgaaga gattcacagg tcagaacaga acggatacga
     4621 actctttcga taccagccga ctgaattttc agggcggttt tttccgtaat ttcctcacct
     4681 acttcaacca aaatctcgtc ttcactttcc ggattataaa tatcttcctg agctactcgg
     4741 ccaataatac gatcggccaa tgattctata atattttctt ccttcttcaa ggcggtgact
     4801 tcgataccgt taatcgtctg gcaatcttcc tcggaaatca caacatcatg agctacatct
     4861 actaatcggc gagtcaggta accggcgtca gccgtcttca aagccgtgtc tgccagtccc
     4921 ttgcgggctc cgtgagtaga aatgaaatat tcgtgaaccg tcaaaccttc tttgaaattg
     4981 gaagtaatcg gcgtttcaat aatttcaccg acttgaccgg tcatagattt ttggggtttt
     5041 gccattagac cgcgcattcc agccagctgc ttaatctgtt caccgcttcc gcgcgcacct
     5101 gaatcagcca tcatatagac cggattgaag ccctgccgat cagtcgccat atgttcaagc
     5161 ataacttccg aaaggcggtt ggtagtgtgc gtccagttat caattacttt gttataacgc
     5221 tcaccttcag tcaatattcc ctttctaaat ttgcgctgaa tatcttctac tctttcaaat
     5281 gattcctgta gaatcttctc cttttcctca ggaattaaca catctgagat agagatcgag
     5341 actccggaac gagtggcata tttaaagccg atatttttca aattatcgag gaacttaact
     5401 gtttcatagt tgccagcttc ctggaatacg ataccaacga ttttttcaag acttctttta
     5461 ctgattaatt cattgtaata ccccattccg ggcggcaaaa gactattgaa taaagcacga
     5521 ccaacagtcg tttcgatgat ttttccatca atcctaattt taaccttggc atgcaaatct
     5581 gcttcgccga tttcataggc gtaaattgct tcttccggtg aagaaaaaac cagaccggcg
     5641 ccccgcgcac cttctttctg gcgggtaatg taataaacac ctaaaaccat atcttgcgaa
     5701 ggaatagcaa tcggatgacc atgcgctgga tgtaaaatgt tatgactgga taacgccaac
     5761 agccgacatt cgatctgggc ttcaaaggta agtggtatat ggacagccat ctggtcgcca
     5821 tcgaagtcgg cattgaatgc cgcacaaact aacggatgaa tttgaatggc tttgccttca
     5881 attaatttcg gctgaaaagc ctgaattcct aaacgatgta gagtcggagc acggttcaga
     5941 agaaccgggt agtccttgac cacatattcc agaacgcgat aaacttcggc atctttgcgc
     6001 tccaccatag ttttagcggc tttgggcgtc ttggctagcc gacggcgaag gagagcgtga
     6061 attatatggg gcttaaagag ttcgattgcc atatctttag gcaaaccaca ttcattgaga
     6121 cgtaaatctg gtccgacgac gataaccgaa cgtccactgt aatccacacg tttccccagt
     6181 aaattctgac ggaaacgccc ttccttgcct ctcagcatat cgctcagact tttgagcgga
     6241 cgacgcgtgc cactgcgaac ttctgttcgg cggcgactat tatcaaataa tgaatcaact
     6301 gcttcctgaa gcattcgctt ttcattgcgc aaaatgacat cgggagcctt gatttcgatc
     6361 aattgtttta aacgattatt gcggataata acccgccgat aaagatcatt taggtcgctc
     6421 gccgcgaatc gtccaccttc cagtggaacc agcggacgca aatccggcgg aatgaccggc
     6481 aaaacggtta ccaccatcca ttccggttta tttatcggct tgccttcttg attcaagaaa
     6541 gccatcacga ctttcaggcg ttttagaatc tcggccttct gcgtcggtga cttttttatt
     6601 tttaattctt ctttcagact atgaactagg ctttctatat ctactttttg gagcagttcc
     6661 cgaatagctt cgcccccggt tttagcaata aaatagttct tagcttcttc ttcatcaaaa
     6721 agctcggtct tcggaccata agcctgcata ttttcaaaat attcttcttc ggtaataacc
     6781 gttttatatt ttaggtcgga tttgccctga tttaaaacta catatctctc gtaataaaca
     6841 atctcttcca aattcttagt cggaatatcc aatagataat ataacttgct aggaattgac
     6901 ttgagaaacc agatatggac aaccggtaca gccagggtaa tatgtcccat tctttcgcga
     6961 cgaacttttt tgcgagcgac ttctacgccg caacgatcgc aaataatgcc tttataccgg
     7021 atacgtttat attttccgca atggcattcc cagtctttca ctgggccaaa aatcttttca
     7081 caaaacaacc catctttctc cggcttataa gaacgataat taatcgtctc tggctttagg
     7141 acttcaccgt gagacctggt taaaatggtt tcaggtgaag aaagatggat cgtaatactt
     7201 tcaaattcgc tacgaaatgt agatggtttt gtataattca tactgataat tatacctccg
     7261 taattggatt aattaatatc catttcaatg cacaagcctt gtaattcttt caataaaaca
     7321 ttaaaagatt ccggcacacc gaaatcggag aaattttcac ctttgacaat ggcattatag
     7381 actttcgatc ggccatcaac gtcatcggat ttgactgtca agatttcctg caaagtgtaa
     7441 gccgcaccat aggcttccag cgcccagact tccatttcac caaatctctg accgccaaat
     7501 tgagctttgc cacccagcgg ttgctgagta actaatgaat acggaccggt ggaacgtgca
     7561 tgcattttgt cttcgactaa gtgtgataat ttcatcatat agatgtagcc gaccgtcacc
     7621 ggattatcaa actgctcacc ggtttgacca tctataaggc taaccttacc aatttctggt
     7681 aatccggctt tgcgcaattc ttcctgaact tcctgctcgc ttgcgccatt gaacacggga
     7741 gtttcataat aaacaccaag ttcgcgcccc gcccaaccta acatagtttc atacaattgc
     7801 cccaggttca ttcgggaagg tacacccatc ggatttagga taatatctac cggtgtaccg
     7861 tcttcgagga aaggcatatc ctctttagga acgattattg agacgacacc tttattacca
     7921 tgacggccag ccattttatc accaacttga actttccgtt tgctggcaat atagactttg
     7981 gctaattgta agacacccgg ctgaaggtca tcgccaactt taatcttata aatggtttgt
     8041 tctaattcct catcgattcg ctgactaaga ataaaataac ttttaacgat tttctcaact
     8101 ttttcgttga tttccggatc gtttgtccag ggcgtttcca ggctaagtag atcgaaatct
     8161 aactgggcta gaatttcatc attaaattgg atgcctgact tgatagcaac tttgccattg
     8221 gttagattct taatcgtggc acttgtagca cccttcagaa tatttttcaa tagaagatcg
     8281 cgtcttcctt taaggtaagc atgcttttga acactgcgtt tggtttcctc gtcaatctga
     8341 cgtttttctt ccatccgact gactttactt ttgcgggtaa aaagtttggt atcaacggca
     8401 acgcctctga tccccggatc agcccgtttg gaggcatctt tgacatcact ggctttctcg
     8461 ccgaaaatcg cccgcagtaa tttttcttca ggagtcggat cggtctcacc tttgggagta
     8521 actttaccaa ttagtatgtc attaggttcg acttctgcac caacccgaat tattccattg
     8581 gcatctaaat tcttagtagc ttcttcactg acattcggaa tttcacgagt cagctcttcg
     8641 tttccacgtt tggtatctcg aacttctagc tcgaattcct tgatatgaat cgatgtaaat
     8701 acatcatcat agaccaagcg ttcgtttaaa ataatggcat cttcgaaatt gtatccgcgc
     8761 cagggcataa aagccaccaa cacattacga ccaagcgcca actcgccatc attagtagca
     8821 caaccatcgg caattgcatc accggctttt actcgatcac cttctttaat caaaggcttc
     8881 tgatttatgc aagtatcctg attagtacgg aggaacttag ctaattcaat acgaacttta
     8941 ccttgatttt catctaaata cagttcgttc tcagtgtcgg tgcgaattac gatgtgatcg
     9001 gcggtaactt cttctacaat gccgtttacc ggggcgagta caactgactg agaatcgcgg
     9061 gctactaccc cttccagacc ggtaccgaca atgggacgct ctggtttcaa taatggcact
     9121 gattgtctct gcatgtttga acccattaaa gcccggttgg catcatcatg ttcaagaaat
     9181 ggaattaatg cggcggagac cgagacaatc tgctccggtg aaacatccat atatttaact
     9241 tcgtcgggtg ttacaattgg gaattcaccc ctaacccttg ccctcacccg agaattaata
     9301 aagtgccctt gttcatccag cggttcatta gcctgagcaa ttatctcgcg gtcttcatca
     9361 tccgctgaca agtactcaac tttatcagtt accgtagcga ttccgttgac tttttcaaca
     9421 cggcgatacg gagtttcgat aaaaccgaga tcattgacat aggcataggt agcgagtgaa
     9481 gcaatcaagc cgatattagg accttccgga gtctcaatcg gacataatcg tccatagtga
     9541 gtatagtgca catcacgtac ctcaaatcca gcacgttcgc gggataagcc gccaggtcct
     9601 aaggatgaga ctcggcgctt atgagtgatt tcagccagag gattggtctg atccataaat
     9661 tgggacaatt gactggtccc gaaaaacgaa tttatcaccg aagttataat gcgtgaatta
     9721 attaaatctt gaggggtgag attttccgat tcgcgtagat tcattctctc tctgattgtg
     9781 cgcgccattc tggtaaatgc cagattaaac tggttggtga gctgttcacc aacgtttcgc
     9841 acccgacgat tcccgaggtg atcaatgtca tctgggccga gattgccctt tttcatatca
     9901 acgatacgct tcataatctc gacgatatca tcgcgagtta gaacacattc ttccagtgga
     9961 actttcagat taaatttttt attaatccga taacggccaa cctgtcccaa atcatacttc
    10021 ttgggactga aaaataagcg ctcaacaaac tttttagcaa tatcgagatt cggtggctcg
    10081 ccagttctta gactgcgata gagaaaatag agcgcagatt cctcatcatt ggtttgagta
    10141 tctttgcgga aggtatttaa aaaaatattg cgctcgattt catcatcggt cgtgaatact
    10201 aatagggaag taattccatg ttgtttgcat tttttgagaa aatctttatc aacgacgatc
    10261 tcttctttga tttcagaaat aacttcgccg gtctcgggat caatgacgtt ttccgccagc
    10321 atttttccca gaagctcttt ggaatctcca tcaagagaaa agcgtttgcc gattccgaat
    10381 aagttgacga tgtcttcatt ggtcggatag ccaaaggcac gcaataaaat ggtcgctgga
    10441 aatttacggc ggcgatctat gatagcactc agaatatcac gagtatcaat tacaaaatca
    10501 atccatgaac cccggaaggg aataactcgt gccgaataaa ttttcatgcc attggtatgt
    10561 tcggtttcat caaagaaaac acctggtgaa cgttgcaatt gactgacaat agcgcgttcg
    10621 gcaccattaa tgataaaggt acctttttct gtcataaagg gaacgtttcc aaagaaaata
    10681 tcctgctcga taccagcctg atactcgcct ttggaaatgc cttcttcact gatatgaagc
    10741 accagacgaa cttttagggg tacagtatag ctgacacctc gttcgagaca ctctttgata
    10801 gtgtaacgcg gttcaccaat tgtgtaatat ttgtactcaa gaagataatt gccgtgggta
    10861 tcttcgactg gaaaaacgtt tctgaacgct gattccagtc caatattttt gcgttccagc
    10921 ggcatcgtat ctttttgtaa aaattcatcg aaagatttta actgcacttc cagcaggtta
    10981 ggcatctttt gcacaatttt aactttggca aaagatttcc tttctttctc taaagggaga
    11041 gtattactca acggtatccc tcctgattaa tttgattttc catacaatac aatgaaaatt
    11101 ccttattggc aaaacaagaa ttctcataaa tgctgaagat tgaaactgag tgtagtttac
    11161 ttgatttcga ctttggctcc ggcttcttcg agttgttttt taagttcagc cgcttcctct
    11221 ttggaaatgc cctctttgac cgtactggga gcgccatcaa ccaaatcttt ggcttctttc
    11281 aatcccaaat tggtagccgc ccgcacgact ttaatcacgt taatcttatt agggccaacc
    11341 tctttaagaa taacattgaa ttcagtttgt tcttcaaccg cttcggcttc agccggggca
    11401 ccaggctgat ctactgtagc cgcaactgcg acgggcgcgg cggctttcac gtcgaatttt
    11461 ttctcgatgt ccttaatgag ttcagaaatt tccagcatat ttgctttttc cagatactcc
    11521 agaacgtctg ctctagttat gttcgccatt tctaaaaaac ctccttttag tgcgatgctt
    11581 atgattgttt tttttgctca tttaatgaat ttagagtgcc gactaactga tttaacgtag
    11641 cacgtagaac catagtcagc tggctcatcg gcgcatttag agtagcgagt agagaagcca
    11701 gcagttcccg gcgggaaggt aaattaatga ctttctcaac ttgatcggct ccaaatagca
    11761 ctccatcata gatacaaccg gtaataacca gcttatccag tttatttttc ttaataaaag
    11821 tgttgatcac tttcccgggt atagcgggat cagtatagga aattgctaac gcagtttggc
    11881 catctaataa tggctcgatc gaatctaatc ctgaattttt agcggccatt ttggtcaaag
    11941 tctttttaac tactttatat tcgacatcag ccgcatataa ttccttacgt aagtcggtga
    12001 tttctttgac atttagacct ttataattgg tgaaatagat accacgggct ttttgaaatt
    12061 tctcactgat cgtttccaga ctctgaatat ttttctgatt tggcattttt gctcctgaga
    12121 ttacgctata attgaggatt tatctacccg tactccggga cccatcgtcg tggttagagt
    12181 cactttttga atgtatgctc ctttaacaga aggtgggcgt aaacgcataa gtgttgagat
    12241 taaagtctgt acattctctt cgagatgctc gattggaaat gactttttac caacgatagt
    12301 atgaacgatt ccggtcttat ctactcggat ttctactcga ccggctttga tttcttttat
    12361 agccgcagcg acatcattag tcacagtgcc acttttggga ttcggcatca aaccttttgg
    12421 tccgagtatt tttcctaatt taccaacttg tcccattaca tccggagtgg caatgataac
    12481 atctacgtca gtccagccgt tttgaatttt ttcgagatat tcttccagac cagcataatc
    12541 tgctccggct tggagggctt catctacttt actcttggtc aatactaaaa ctcgaacttt
    12601 gcggccggtg ccgtgcggat aggttactgt gcccctgata ttttgatcgg catgacgcgg
    12661 atcaactccc aaattaacat gggcttcgac ggtttcatcg aatttggctc cgttgcaatt
    12721 ttttagtaac tcgagtgctt cccgcagagg gtattccttc ttccggtcaa cttttttact
    12781 gttctgttca actcgctttg aaatgttcat aacgttgaat cctcttactc atttattgaa
    12841 atgcccatac tccgggcagt acctttaatc atttccgcgg ctttttcaac cgtgtgggca
    12901 tttaaatcag gcatttttat tttagcaatt tccatgactt ggtccattgt taccgatccg
    12961 attttacttt tattaggttc acctgagcct ttttctactt tggcggcttt caatagcaaa
    13021 acggatgccg gcggtgtctt ggttataaaa gtaaatgaac gatctgagta aaccgtaata
    13081 actacgggaa tgatataacc agcttgatcc tgagtgcgag cattaaacgc cttacagaat
    13141 tcaacgatat tgacactatg ctgacccaat gccgggccta ctggaggtga cggattggct
    13201 ttgccagctt cgatttgcaa tttaatggtg gctaagactt ttttcgccat attacaccta
    13261 tttctccaat tctacttgta aaaagtccaa ctcgactggt gttggacgcc caaaaatact
    13321 tactgaaact tttaatttac gtttatcttc attgacttcc tgaactactc cgcggaaatc
    13381 tataaaaggg ccatcaatga ctttaaccgg gtcacccatt ttaaacggaa ccgctaacac
    13441 ctcccggccg tctttcttct caacttcgcc catcactcgt ttaacttcat cttctttcaa
    13501 aggctgaggt ttaccggcgg ctcccacaaa gttcattact ccgggaatat tctcaatcag
    13561 atattgcgac tctttagtga tatccatatt gaccaaaata tagccgggaa agaatacttt
    13621 gttgcgaacg cgtttcttac cctcacgcat ttccataata ttttctgaag gcaccagtac
    13681 atctttgata atatctttca tcccagcatg ctcggcttca atcagaatag cttctctgac
    13741 ttttttttcc ttaccggaat atgttcttaa tacataccat tccatatcgg acctgctaaa
    13801 aaattacatt cataattttt gatagaatca gatcaacaac gaaaagaaat accgataaaa
    13861 tcaatgagaa aatcagaaca acggcggttg aacttttaag ctccgaccag gtcggccagg
    13921 agactttttt catctcgaag acaacccctt caaaaaattc gcgaatgcgt ttgatcatgt
    13981 ctttgtcctc cgtttgggcg atcggtaagg tctattagca atattgcagg tgaggcagga
    14041 attgaacccg caacccccgg ttttggagac cggtgctctg ccagttgagc tactcacctt
    14101 tagttgcata ttaacgggtc tctttatgaa ctgtatgttt tttgcaccaa cgacagtatt
    14161 ttttatattc gacccggtta ggctgtagct tcttattctt atcagaagaa taatttcgac
    14221 gtttacaaac cgtgcattcc agagtaatta attcacgcat attattccct gaaatttatt
    14281 caatgatttc ggtaacgacg ccagcaccaa ctgtccgtcc accttctcgg
//
LOCUS       DAOMJU010000048        13547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_4516_length_13547_cov_23.260599, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000048 DAOMJU010000000
VERSION     DAOMJU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13547
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4516_length_13547_cov_23.260599"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1441
                     /locus_tag="PLO58_05960"
     CDS             188..1441
                     /locus_tag="PLO58_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQO74368.1"
                     /translation="MARKISILMLIAVLNAVSLAENLFSNVLNISADSTGNVLLVYKS
                     HQKMFVVRSNAPGALEIVKEYRITTGRRRGDKERSGDLKTPEGIYYIIGRVPDNRLTA
                     RYGPLAYALDYPNYVDRLQKRNGSNIWIHGRDEEIKDFITEGCISLENSHILDLRKYI
                     VVRQTPVIILDNLSDYYVSPENLKAAVSFWAEYINGWTKNWDSGAVAEYCDYYAPNFL
                     DENGRNLKQFKSYKTDLEKRYQWKTVMVDEISVLVSDCEAHLKFRQRYLCPTFYSEGQ
                     KQLILVPIDSNWRIVRETFRPISPSLTTEEMVTQFLSGWKSAWEQKDIDTYINFYAEE
                     FTSGEYDLSGWKNYKDQVFQSVQNISVKWSALRIQSPQEQVWKVTFRQIYQGDGYRDI
                     GLKTLTLKGFPGNIKIIGEEWVEEK"
     gene            1428..2879
                     /locus_tag="PLO58_05965"
     CDS             1428..2879
                     /locus_tag="PLO58_05965"
                     /inference="COORDINATES: protein motif:HMM:NF028343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14/M99 family metallopeptidase"
                     /protein_id="HQO74369.1"
                     /translation="MKKNKTRKTILLIGLIISWQVASGLTAEIKRPSREHEVYFENTP
                     NELHVYKLYGRFDGNTIFILGGIQGDEPGGYLSADLYPDLVLDRGNLIVIPRANFHSI
                     ILNKRGVNGDMNRRFDRKQSTDIEDQIVAIIKQLMSESDLFLNLHDGSGFYSKTYIDK
                     NRNPYCYGQSIIADADQVVLNGDTLKLEEMAQKVIKGINLRVENPEHKFSFFNTNTLS
                     PDTKYPEQRKSATYYALTTFHIPAFGIESSKNLENVELKIRYHNYAINEFFKLMGVEP
                     EHPAVLFEPPKLIYLLITINNTETKIVENDGFIQVNYGDIIRVKHIESNYNRGLTCDV
                     LGIGNAQDFQKPIPVLKPTSIIVRRDNIIIGSVKVETIGSTFDSIAYIIEVNGIQRKL
                     TDGQTLTVKRGDLIKIIEVLIPGLDSADLQVNLKGFVPPGSYNDGEDRNCLVDTRKLQ
                     WKKYSVDGLGKKYPIVVTYGSDELTRAFIYIQD"
     gene            2994..4907
                     /gene="secD"
                     /locus_tag="PLO58_05970"
     CDS             2994..4907
                     /gene="secD"
                     /locus_tag="PLO58_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQO74370.1"
                     /translation="MFKKNIVRFIIIGVVVVLACYSLYWTVAYHSFSDEKREAMRADG
                     SLDKYERRVIRLGLDLQGGMHIVMEVDLPKLIESLASNKTPQFMALLKEANDEAIANE
                     EDFFDVFRRKINESDLKLVRHFNGRGFKNDEILKSLEEEAKDAVDRAMEVIRNRVDQF
                     GVSEPSIQKSGKYRIIVELAGIQDIGRARELIQSTALLEFILVKDAAVTQQFITAVDN
                     YLKTGKAEEVSPETHEEADSIVALKESKDKAISVDELLGTTSTETGLTAEQADTGLIV
                     DEEIYKERPFSSLLRNIDNQIGVPERNVYAVKKILEDPNVKKLIPFDSKFLWSAKPVR
                     RTLQDGRTENFYILYHVHSEPALLGKYITKASASLGGAGSQSSGQPIVNISMNSEGAR
                     IFAKVTGANIGKQLAVVLDDKVYTAPYIRVKIPDGSAYIEGMSDMEEAKDIAIVLRAG
                     ALPAPMTIVEERTVGPSLGKDSIAVGIRVGIFALLVIMLFMFFYYRRSGLLADMALIL
                     NMVFTLAVMAMLRATLTLPGIAGLVLTIGMAVDANVLIFERIREELERGKTVSAAVST
                     GYSRALSAILDSNITTLLTAIILMQTGTGPVKGFAVTLFWGIASSMFTAIFVTRTFFN
                     WRIEKHAIKTLSI"
     gene            4925..5806
                     /gene="secF"
                     /locus_tag="PLO58_05975"
     CDS             4925..5806
                     /gene="secF"
                     /locus_tag="PLO58_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQO74371.1"
                     /translation="MRRIIKKTDIPFIKYSKTAITISLVIIAIGLISLIFRGLNFGID
                     FTGGTLIQVKFEQPRTIEDIRNDLATINLENSIIQKSGENEYIIRVPQDLSSANLVDV
                     IKSTLGISDQNILRQEQVGPKIGKELQGAALLAIFFSLIVMGIYIAIRFKFQYSIGVI
                     LSLLHDVLITLTVFTIFQLEVSLSTIAAFLTIIGYSLNDTIVVFDRVRENLKLHRNET
                     YERIMDISINETLSRTIITDMTTLFVVLCLLLVPGEVRIFAIAMTIGSIAGTYSTIYI
                     AGPLVVFWSRRFKRSTK"
     gene            5874..7124
                     /locus_tag="PLO58_05980"
     CDS             5874..7124
                     /locus_tag="PLO58_05980"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011612739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQO74372.1"
                     /translation="MPLTAVIGAQWGDEGKGKIVDILSESADLVARFQGGANAGHTVQ
                     IGTEETILHQIPSGILRSQTHCLLGNGMAFDPVGLKNEIDVVQRKGIDVRNRLHISPL
                     AQVVTPLHKILDNYYENISGARAIGTTKRGIGPCYTDKISRNGIRVCDLFNENDLREN
                     ITSRTIFMRKQGVLTESEEDKLADDIEVFLDAVREILPYVDNTIFRIHSYLESGKNVL
                     AEGAQGVLLDIDFGTYPFVTASNTIAGNVATGLGIGPHWIDSIIGVFKCYTTRVGAGP
                     FPTELNDAVGDHLQARGDEFGATTRRRRRCGWFDAALGKYAVKINGFSSLAFTKLDVL
                     DDLAEIKICTHYANGEFPLLDLKNAIPVYLTLPGWQKSTTEARHLDELPFQARQYLQT
                     LEDLLEVKISYISTGKERHQIILV"
     gene            7361..8380
                     /gene="ruvB"
                     /locus_tag="PLO58_05985"
     CDS             7361..8380
                     /gene="ruvB"
                     /locus_tag="PLO58_05985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQO74373.1"
                     /translation="MHSKMIDPQKLAEDYEYEKTLRPSAFEEFVGQKNVVENLKLYIS
                     AAQERGEALDHVLLFGPPGLGKTTLAYIVAKELGVNIRTSSGPVVERAGDLAGMLTNL
                     AARDVFFIDEIHRLNNVVEEYLYSAMEDYHIDIVIDKGPNARSIQLNLEQFTLIGATT
                     RLGHLTSPLRDRFGVILRMDYYNPDELFQILMRTAQILNIELRPDGAAEIAKRSRGTP
                     RVANRILRRTRDYAQVKAAGVIDKKVAEQSLKMLEIDELGLDNLDRQILLTIIDKFSG
                     GPVGLNNLAVAVGEEAETIEDVYEPYLIQQGLIQRTARGRQATPATYEYFHKNISSSQ
                     QKLFT"
     gene            8392..9426
                     /gene="queA"
                     /locus_tag="PLO58_05990"
     CDS             8392..9426
                     /gene="queA"
                     /locus_tag="PLO58_05990"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQO74374.1"
                     /translation="MRLSDFDYNVPEELIAKYPVSKRDHSRLMVIDRKKQTITHKRFY
                     NIIDYLNPGDLLVINETKVYPARLWAVKDRTEAKVEVFLLRELENSLWEVMVKPARKV
                     RIGNKLTIAKGVQCDVIDNTVSGGRVVRFNNIPKETLYKLIDEVGLSPLPPYIDREPT
                     EDDKETYQTVYAKKRGAVAAPTAGLHFTEELMQKIKDKGVKIFPIVLHIGLGTFRPVT
                     VEDLSRHRMDSEYFEVSAETALAINNARSKSKRIIAVGTSVVRTLETVTVSGFQITPR
                     RGWTDKFIHPPYEFKMVDALITNFHQPKSTTIMQVSAFGGHELIMRAYQEAIEKKYRF
                     FSYGDAMLII"
     gene            9437..10141
                     /gene="ispD"
                     /locus_tag="PLO58_05995"
     CDS             9437..10141
                     /gene="ispD"
                     /locus_tag="PLO58_05995"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQO74375.1"
                     /translation="MSLNTPFISGIVVAAGQGKRFGLGIPKQFLKIKGQPLLAYTIDN
                     LLKIGILSELIIVVSDDWINSKELKACLPLPSSVPIKIIAGGEHRQDSVFNGMQVIDS
                     QAEIVVVHDGVRPFVMPELVRETVRLCSEFDGAILAIPAVDTLKKVEDGQIVRTIERS
                     RVWQAQTPQTFRREVLERAYANARQNNIIATDDAMLVELIGGKVAVVKSTPDNLKITC
                     QNDLKIAKSILKLREP"
     gene            10143..10622
                     /gene="ispF"
                     /locus_tag="PLO58_06000"
     CDS             10143..10622
                     /gene="ispF"
                     /locus_tag="PLO58_06000"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQO74376.1"
                     /translation="MRYRFGSGIDVHRFCSGRPLILGGVTIPFDKGLLGHSDADVLTH
                     AIIDALLGASALGDIGTHFPDTDAQYKDISSLILLEKTREMVVSVGFRVSQIDATVIT
                     QAPRLLPFIPEIRHSLADCLQIPVGDISVKAKTTEGLGFIGSGEGIAALATVTLITL"
     gene            10619..11242
                     /locus_tag="PLO58_06005"
     CDS             10619..11242
                     /locus_tag="PLO58_06005"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HQO74377.1"
                     /translation="MNALAEFLTTGSGWIVYVVLFILIFLENVIPFIPGDTVLAFSSY
                     LAGIGILNSFISYPLTVIAAVVGFIFTYVIAYFWGRAFIDRKNFPAFPPRKMARIDRL
                     FHKYGYMGLGIGRFLPGTRLLMAFMAGFTRLRMVPALACISFGIIGWNMLIYLLGYLV
                     GGNRQAIAAFISQYNHIASLIVLALIVILIAWRVNQKIKKLGEKTSD"
     gene            11235..12734
                     /gene="gltX"
                     /locus_tag="PLO58_06010"
     CDS             11235..12734
                     /gene="gltX"
                     /locus_tag="PLO58_06010"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQO74378.1"
                     /translation="MIRTRFAPSPTGFLHVGGLRTALYNFLFAKKYNGRFILRIEDTD
                     QRRKVAGAVENLLATLEWAGVKPDEGPQIGGDFGPYVQSERLQLYRDYAQLLLEKGHA
                     YYCFCSAERLKKLKEWQIAQKIPPRYDNHCRDLSVEEIQQHLSSGEEYVIRMKIPAAG
                     AIEVDDLIRGKVTFPVSQIDDQVLLKSDGYPTYHLANVVDDHLMQISHVIRGEEWLPS
                     TPKHVLLYRYFGWELPQFAHLSLLLNPDKSKLSKRQGDVAVEDYRRKGYLPEALINFL
                     ALLGWNPGDDREIFSLDELIREFSLERVSHSGAIFNIEKLNWMNGIYIRNLSLERLWE
                     YAKPYLEECLHQPCVDERAHWDEARLKAVLNSVKDGLSNFSEIPQKTAFYFASSISYE
                     APEIQDYLTTPESITVLKTLIPLIDSVGINQTDGKKPDFNSDSFIAAVRNVQKATGIK
                     GKPLWMTIRVALTGTTQGPEIHQIASIIGRETCIKRLEAALNYAESHLN"
     gene            12712..>13547
                     /gene="ispE"
                     /locus_tag="PLO58_06015"
     CDS             12712..>13547
                     /gene="ispE"
                     /locus_tag="PLO58_06015"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQO74379.1"
                     /translation="MPKATSIKIKSNAKINLTLRITGRRPDGYHNLSTIFQEIDLHDV
                     LTFTPAKTFEFATNHLALSNKTDNLCAKAYRRMEQIAPHSQPWRITLEKNIPIGAGLG
                     GGSSNAATVLKFLNKAWQINLSDDNLARIGCAIGSDVPFYIKGKTQGARGIGEVLEPL
                     ILMEDFVILLVLPEINISTAWAYRQFNPKNAKPAYNFAELISGGRVDWVKFENQFEEI
                     VFPVYPQLAKLKEQILNTGALYAGLSGSGAALFGIFRNRTYALKAARQLPEWRTIITK
                     PV"
BASE COUNT         3861 a         2773 c         3067 g         3846 t
ORIGIN      
        1 ggtattaagt taagcctcgt tgctatgatt ttgcctcagt tttaatctta acctcgacct
       61 tagtctcagc ctcaacttat cttaccaacg aaaattgcct attttaaaaa acgattttat
      121 tatctaattt tggcgcactt taattatgaa tatcagtgat gtaaacttaa aattcagaaa
      181 tacaaatatg gcgcgaaaaa tttcgatctt gatgttaata gctgttttaa atgccgtaag
      241 tctggctgaa aatctattct cgaacgtgct caatatttca gccgactcaa ccggtaatgt
      301 cttactcgtt tataagtcac accaaaaaat gttcgttgta agaagtaacg cgcccggcgc
      361 tctggaaatt gtaaaagagt accgaattac aaccggaagg cggaggggtg ataaggagcg
      421 ttcaggcgat ctaaagacac cggaaggtat ttattatatc attggtcgag taccggacaa
      481 tcgcttaacc gctagatatg ggccgctggc ttatgcgctg gattatccga attatgttga
      541 tcgtttacag aaacgtaatg gatcgaatat ttggatacat ggacgcgacg aagagatcaa
      601 agattttatt accgaaggat gtatttctct tgaaaatagt catatattag atctccgaaa
      661 atacatcgta gtccgtcaga ccccggtaat cattttggat aatttaagcg attattatgt
      721 atctccggag aatttaaaag ccgctgtaag tttctgggca gagtacatca atggttggac
      781 gaaaaattgg gacagcggcg cagtggcgga atattgtgac tattatgccc ctaacttcct
      841 tgatgaaaat ggtcgtaatc tgaaacaatt taagtcctat aaaactgacc tggaaaaaag
      901 ataccagtgg aaaactgtaa tggttgacga aatcagtgta ctagtctctg attgtgaagc
      961 gcatttgaaa tttcgccagc gctatctttg cccaacattt tatagtgaag gccagaagca
     1021 gttaatttta gtgccgattg attcgaattg gagaattgtg cgggaaactt ttcgtccgat
     1081 cagcccaagt ctgacgaccg aggaaatggt tacccaattt ttatccggct ggaagagtgc
     1141 ttgggagcaa aaggatattg atacttatat aaatttttat gcagaagagt tcaccagcgg
     1201 cgagtatgat ctatccggct ggaaaaatta caaagaccag gttttccaat cagtgcaaaa
     1261 tatcagtgtg aaatggagtg ctttgagaat tcaatcgcct caagagcaag tctggaaagt
     1321 aactttcaga cagatttatc agggagatgg ttatcgggat attggcttaa agacgctaac
     1381 acttaaaggt tttccgggaa atatcaagat tattggggaa gagtgggttg aagaaaaata
     1441 aaacccgaaa aacgattctg ttaattggac tgattatttc ctggcaagtc gcgtctggct
     1501 taactgccga aatcaagcgt ccatcgcgag aacatgaggt atattttgag aatacgccca
     1561 acgaactgca tgtctataaa ctttacggac gcttcgatgg caacactatt tttatcctgg
     1621 ggggtattca gggcgatgaa cctggtggct acttatcagc tgacctttac ccagatctag
     1681 tgcttgatag gggtaatctg attgtcatcc cgcgcgccaa cttccattca ataattctta
     1741 ataagcgggg cgttaatggt gatatgaacc gacgcttcga ccgtaaacaa tccacagata
     1801 ttgaagatca aatagtagca attattaagc agttaatgtc cgaaagcgat ctttttctca
     1861 atttgcacga tggtagtggt ttttacagta aaacttatat tgacaagaac cgcaatccgt
     1921 actgctatgg acagtcaatt atcgccgatg ccgaccaggt ggtactcaat ggtgataccc
     1981 tgaaactcga ggaaatggct caaaaagtaa taaaaggtat caacttacga gttgagaatc
     2041 ccgaacacaa atttagtttt ttcaatacta ataccttaag tcctgatact aaatacccgg
     2101 agcagaggaa gtcggcgacc tattatgcgc taacaacttt tcacattcct gccttcggta
     2161 ttgaatcatc gaaaaatctc gaaaatgtcg aactaaaaat tcgttatcat aattacgcga
     2221 ttaatgaatt ttttaagctg atgggagttg aaccggaaca cccagccgtc ctatttgagc
     2281 cacctaaact gatttacctc cttatcacta ttaataatac tgagacgaaa atcgtcgaaa
     2341 acgatggttt tattcaagtc aattatggtg atattatacg tgttaagcat atcgaatcga
     2401 attataatcg tggtcttacc tgcgatgtgc tcggcatcgg gaacgctcaa gatttccaaa
     2461 aaccgattcc cgttttaaag ccgacctcca tcatcgtcag acgtgataat attataattg
     2521 gcagtgttaa agtcgagaca atcggttcga cgtttgacag cattgcctat ataattgaag
     2581 ttaatggaat tcagcgtaag ctaaccgatg gtcaaacgct aactgtcaaa cgaggagatt
     2641 taattaaaat cattgaagtt ttaattcccg gattagacag tgccgatttg caggttaatc
     2701 taaagggctt tgtgccgccg ggtagttata acgatggaga agatcgcaat tgtctggtag
     2761 atacccgtaa attgcaatgg aagaaatatt cagtagatgg tttaggaaaa aaatatccga
     2821 ttgtcgtaac ctatggctct gacgagttga cacgagcctt tatttatatt caggattgaa
     2881 atttggattt atttgaccag gctggctttt tgtttgacat taaacgactc ttgaagttaa
     2941 attcacatgc tttttttaaa ctaaatataa gatatacttt gaggtgattc tgaatgttta
     3001 aaaagaatat tgtccgattt atcattatcg gtgtagtagt tgttctagcg tgctattcat
     3061 tatattggac agtcgcttat cattcctttt ctgacgagaa acgggaagcg atgcgggcgg
     3121 atggttcgct ggataaatac gaacgccggg tcattcggct aggccttgat ttgcagggcg
     3181 gtatgcatat tgtgatggaa gttgatctgc caaagttgat tgaatctctg gcatccaata
     3241 agacgcccca gtttatggct cttctaaagg aagctaatga cgaagccatc gctaacgaag
     3301 aggatttttt cgatgtattt cgtcgaaaaa tcaatgaatc ggacctaaag ttagtgcgtc
     3361 attttaatgg acgcggattc aagaacgacg agattctgaa gagtctggaa gaagaagcca
     3421 aagatgccgt tgaccgcgct atggaagtga ttcgtaatcg cgttgatcaa tttggcgtat
     3481 ctgaaccttc gatccagaaa tctggcaaat accggattat tgtagaacta gctggtattc
     3541 aagatattgg tcgggctaga gaattgattc aaagtacggc tttactggaa tttattctgg
     3601 taaaagatgc ggcagttacc cagcaattca taacggctgt agataattat ttaaaaacgg
     3661 gcaaggcaga agaggtctcg cctgaaactc acgaagaagc cgattcaatc gtagcattga
     3721 aagaatctaa ggacaaggcg atcagcgttg atgaactact gggaaccact tctaccgaaa
     3781 ccggacttac cgccgaacag gctgataccg gcttgatcgt tgatgaggaa atttacaaag
     3841 aacgcccgtt ttcttcttta cttagaaata ttgataacca gattggagtt ccggaacgca
     3901 atgtttatgc tgtcaagaaa attcttgaag accccaatgt gaaaaagttg attccttttg
     3961 attctaaatt tctctggtca gccaaacctg tacgtcgtac tcttcaggat ggtcgtactg
     4021 aaaattttta tattttatac cacgtccata gcgaaccggc tttactaggc aaatacataa
     4081 ccaaagcttc tgcttcgctg ggcggtgctg gttcgcaaag cagtggccag ccgatcgtga
     4141 atataagtat gaatagcgag ggcgcccgca tatttgcaaa agttaccggc gcgaatatcg
     4201 gcaaacagct ggcggtagtc ctcgatgata aagtttatac tgcgccttat ataagagtta
     4261 agataccgga cgggtcggct tatatcgaag gtatgtccga tatggaggag gctaaagata
     4321 tcgctattgt gcttcgcgct ggagcattac cagcccctat gactattgtc gaggagagaa
     4381 ccgtcggacc gtcgctcggt aaagattcta tcgccgtggg tatccgggtt ggtatttttg
     4441 ctctgttggt tataatgctg tttatgtttt tttattatcg gcgttctgga ctattagcgg
     4501 atatggcttt gatcctgaac atggttttca cactggctgt tatggcaatg ctacgagcga
     4561 ctctaactct gcccggtatc gctggtctgg tgttgacaat tggtatggca gtggacgcca
     4621 atgtattgat ttttgaacgt attcgcgagg aactggaacg tgggaaaact gtaagtgcgg
     4681 cggtcagtac cggttactcc cgggcattgt ccgccattct ggattcaaat attacaactc
     4741 tgctaacggc tataatattg atgcagaccg gaacaggccc tgtcaaagga tttgccgtaa
     4801 cgcttttctg gggcatcgca tccagtatgt tcacggctat attcgtgacc cgcaccttct
     4861 ttaactggcg aatcgaaaaa catgctatta aaactcttag catttaagat caggataaat
     4921 tgatatgaga agaattatta agaaaactga tattcctttt attaaatata gcaagaccgc
     4981 tataacgatt tcgctggtaa taatcgctat cggccttata tcgttaattt ttcgcggatt
     5041 gaactttggg attgatttta cgggcggcac tctaatccag gtcaaatttg aacaacctcg
     5101 aacaatcgag gacattcgca atgacctggc aactatcaac ctcgaaaaca gtattatcca
     5161 gaaatctggc gaaaatgaat atatcatccg agtaccgcaa gatttatcca gtgctaatct
     5221 ggtagatgtt atcaaatcta ctcttggaat tagcgaccag aacatcttac gccaggaaca
     5281 agtaggtcca aagatcggga aagaactgca gggagcggct ctactggcta tctttttctc
     5341 tcttatcgtt atgggtattt acattgctat ccgatttaaa tttcaatatt ctatcggtgt
     5401 gattctctcg ttgctccacg acgttttaat taccctaacg gtttttacta tttttcagct
     5461 ggaagtatcc ctatcaacaa tcgcggcttt tctgacgatc atcggctact cactaaatga
     5521 tacaatcgtc gtatttgacc gcgtgcgcga aaacttgaaa ttacatcgta acgaaaccta
     5581 tgagagaatc atggacatca gtatcaacga gactcttagt cgaacgatta ttaccgatat
     5641 gacgacttta ttcgtggtct tatgtctact tttagtccca ggtgaagtgc gcatctttgc
     5701 gatcgccatg acaattggca gtattgccgg gacatattcc acgatttata ttgcaggtcc
     5761 gttagtggtt ttctggtcgc gtaggttcaa acgctccact aaatgattca tataaaacaa
     5821 tcaatgaaaa atgctccctt tctcgggagc attttatttt ttaggagaat aaaatgccgt
     5881 taactgccgt gattggtgcc caatggggcg atgaaggcaa aggtaaaatc gttgacattc
     5941 tgagcgagtc agccgatttg gtagcgcgct tccagggcgg cgctaacgcc ggtcatacag
     6001 ttcagattgg cacagaagaa accattctgc atcaaattcc cagcgggata ttgcgatcgc
     6061 aaacgcattg cctccttggc aacggaatgg ctttcgatcc ggttggtctc aagaatgaaa
     6121 tagatgtcgt ccagcgaaaa gggattgacg tccgtaatcg tctgcatatt tcgccactgg
     6181 cgcaggttgt tacgccacta cataaaatct tggataacta ttatgaaaat ataagcggcg
     6241 cccgagcgat tggcaccacc aaacgcggaa tcggcccatg ttataccgat aaaatcagcc
     6301 gcaatggtat tcgagtatgc gatttattca atgaaaatga cctaagagaa aacattacct
     6361 ctcgaaccat ttttatgcga aagcagggag ttttgacgga atcagaagag gataaattgg
     6421 cagatgatat tgaagtattt ttggatgcgg tgcgcgagat acttccctat gtggacaata
     6481 cgattttccg aattcactcc tatttagaaa gcggaaaaaa cgttttggct gaaggagctc
     6541 agggagtact gttagatatt gactttggca cttacccctt tgtaacggcg tcgaatacga
     6601 ttgccggtaa tgttgctacc ggattgggta tcggaccaca ctggatagat tctataattg
     6661 gagttttcaa atgttatact acccgggtag gtgcaggtcc ttttcccacc gaactgaatg
     6721 acgcagtcgg agaccattta caggcgcgtg gtgatgaatt cggtgctaca actcgccgtc
     6781 gccgccggtg cgggtggttt gatgccgccc tcggcaagta tgcggtcaag atcaatggat
     6841 tttcatctct ggcattcact aaactggatg tgctggatga tctggcagag ataaaaattt
     6901 gtactcatta cgcaaatggc gaatttccat tattagattt aaagaatgct attccagttt
     6961 acctgactct tcccggctgg caaaaatcca cgactgaagc ccgccattta gacgagttgc
     7021 cttttcaagc tcgccaatac ctccagactc tggaagatct acttgaggtt aaaatttcct
     7081 acatctctac gggcaaagaa cgtcatcaga ttattttagt ataaacaatc cagatcccta
     7141 attattcgtc ggtaaatcta taatacagca ttgctttacg gcttattttt aagtaaatta
     7201 tgccaaattt tgtggtaatg tattgctaaa gaatataaag aattttaaat cactactgga
     7261 atttatttta cttatttcaa cacgaattaa cctgcaactg caatattgtt ttaaaccgac
     7321 aatattaaaa tatcaataaa acgaaaatca ggattttcgg atgcattcta aaatgattga
     7381 tccccaaaag ctggccgaag attacgaata tgaaaaaact ttacgccctt cggcatttga
     7441 agaattcgtc gggcagaaaa atgtggtgga aaatcttaag ctatacattt cagcggcgca
     7501 ggaacgcggt gaagcgcttg atcatgtcct cttattcggg ccgcccggtt taggcaaaac
     7561 tactcttgcc tacatagtgg ctaaagagct gggagtaaac attcgcacca gttccggacc
     7621 ggttgtagaa cgagctggcg atctggctgg tatgctaact aatttggcgg caagagatgt
     7681 atttttcata gatgaaattc atcgcctgaa caacgtggtt gaggaatatc tttattctgc
     7741 catggaagat taccatattg atattgtcat tgataaagga ccgaatgctc gcagtatcca
     7801 gcttaacctc gagcaattta cgctaattgg agccaccacc cggctgggac atttgacctc
     7861 gcccttgaga gaccgctttg gagtaatttt gcgaatggat tattataatc cggacgaact
     7921 attccagatt ctgatgcgta ccgcccaaat tttaaacatt gaactgcgcc cggacggcgc
     7981 cgccgaaatt gccaagcgta gccgcggaac accgcgcgtc gctaatcgga ttttacgccg
     8041 cactcgcgat tatgcccagg tcaaagccgc tggcgtaatt gataaaaagg tcgccgaaca
     8101 atccctgaaa atgctggaaa ttgatgaatt aggactggat aatttggacc gccagattct
     8161 tttaactatc attgataaat tttccggcgg accggtcggg ttgaataatc ttgcggtcgc
     8221 agtcggtgaa gaagctgaaa ccatcgagga tgtttatgaa ccttacctga ttcagcaagg
     8281 attaattcag cggaccgctc gcggacgcca ggcaacacca gcaacctatg aatatttcca
     8341 taaaaatata agtagctctc agcaaaagct gtttacgtag gaggcaaata aatgcgactt
     8401 tccgattttg attataatgt gccagaggaa ttgattgcta aatatcctgt aagtaagcgc
     8461 gatcattccc gattaatggt cattgaccgt aaaaaacaga ctatcactca caagagattt
     8521 tacaatatta ttgattatct caatcccggt gatttgctgg ttatcaatga aaccaaagtc
     8581 tatcccgctc ggctttgggc ggttaaagac cgtacagaag ctaaagttga agtatttctt
     8641 ttgcgcgaat tggaaaatag tctttgggaa gttatggtca aaccagcccg caaagtccgg
     8701 attggcaata aattgaccat tgctaaaggt gttcagtgcg atgttattga caataccgtt
     8761 tcaggcggtc gggtcgtgcg ttttaataac attcccaagg aaaccctcta taaattgatt
     8821 gatgaggttg ggctatcgcc tctgccgccc tatattgacc gcgagccgac tgaagatgat
     8881 aaggaaacct accagactgt ctatgccaag aaacgaggcg cagttgccgc tcccaccgcc
     8941 ggtttgcatt ttactgaaga attaatgcag aaaattaaag acaagggcgt taaaatcttt
     9001 cccattgttc tgcacattgg tttaggtact ttccgcccgg tgaccgtcga ggatttgtct
     9061 cgtcatcgga tggattccga atattttgag gtttcagctg aaacggctct cgctataaat
     9121 aatgcccgct ccaaaagtaa aaggatcata gcggtaggta cttccgtcgt gcggacgctt
     9181 gaaacggtca cagtttcagg tttccagatt acaccgcggc gtggttggac cgataaattt
     9241 attcatccac cgtacgagtt caagatggtt gatgctctga ttaccaattt tcatcaacct
     9301 aaatccacca cgattatgca ggtttcggct ttcggtggcc atgagctgat tatgagggca
     9361 tatcaagaag ccatcgagaa gaagtatcgc ttcttcagct atggcgatgc aatgcttatt
     9421 atctagttta aatatcttgt cccttaacac tccattcatt agtggaattg tggtagccgc
     9481 cgggcaaggg aaacgctttg gtttagggat ccccaagcaa tttttaaaaa taaaaggtca
     9541 gccgctccta gcttatacca ttgataatct gttaaaaatc ggtatcctta gtgaactaat
     9601 tattgtcgtt agtgatgatt ggatcaattc caaagaatta aaagcctgtt tgccattacc
     9661 gagtagcgtc ccgatcaaaa tcattgccgg tggtgaacat cgccaggatt cggtctttaa
     9721 cggtatgcag gtcattgatt cacaagcgga gattgtagtt gttcacgacg gagtccgtcc
     9781 gtttgtaatg ccagaactcg ttcgagaaac agttcgcctt tgctcagagt ttgacggggc
     9841 aattctggca atcccggctg tggatacttt gaagaaagtt gaggatggcc agattgtaag
     9901 aacaatagaa cgtagtcgag tctggcaggc tcaaacaccg caaaccttcc gtcgggaagt
     9961 gcttgagcgt gcctacgcca acgctcggca aaataatatt atagccaccg atgatgccat
    10021 gttagtggaa ttaatcggtg gaaaagtagc ggttgtcaaa agcacgcctg ataatttaaa
    10081 aattacttgt caaaatgact tgaaaattgc aaaatcaata ctgaaactga gagagccttg
    10141 aaatgagata tcgttttggc agtggaatag atgttcatcg cttttgtagc ggtcgccctt
    10201 tgattttggg cggagtaacc atcccgtttg ataagggctt actgggacat tccgacgcag
    10261 atgtattgac tcacgccatc attgatgctc tattgggagc ttctgcgtta ggcgatattg
    10321 gcactcattt ccccgatact gatgcgcaat ataaagacat ttccagtttg attcttctgg
    10381 aaaaaacccg tgaaatggtc gtctccgtcg gctttcgcgt cagccagatt gatgccaccg
    10441 tcatcactca ggcaccgcgg ctattgccgt ttattccgga aattcgccac agtcttgctg
    10501 actgcttaca aataccggtc ggtgacattt ctgtgaaagc caagacgacc gaaggactgg
    10561 gtttcattgg cagtggcgaa gggatagcgg ctttggcgac ggtaactctt ataactttat
    10621 gaatgcttta gctgaatttt taacgacggg atcaggctgg atagtctatg tcgttttatt
    10681 tattttgata tttttggaaa atgtcatacc gttcattccg ggtgatactg tgctggcttt
    10741 ctcctcatat ttggcaggta ttgggatatt gaattcattt atttcttatc cactaactgt
    10801 catagcggcg gtcgtcggtt ttattttcac ttatgtaatt gcttattttt ggggcagagc
    10861 gtttattgac cgcaaaaatt ttcccgcatt tccgccccgc aaaatggcaa gaattgaccg
    10921 gctttttcat aagtatggct atatggggtt gggcataggg cgatttttgc ctgggacgcg
    10981 gcttttgatg gcttttatgg ccggttttac gcgtttgaga atggttccag cgttagcgtg
    11041 tatctcattc ggtataatcg gctggaatat gctaatttac ctattggggt accttgtagg
    11101 aggaaatcgt caggcaattg ccgcttttat atcgcaatat aatcacatcg ccagcttgat
    11161 cgttttagca ttaatagtaa tactgattgc ctggcgtgtg aatcagaaaa taaaaaaatt
    11221 aggtgaaaag actagtgatt agaactcgct tcgcacccag cccaactggc tttttacacg
    11281 ttggcggttt acgtactgca ctctataact tcttatttgc caagaagtat aacgggcgtt
    11341 ttatcctgcg cattgaagat accgatcaac gtcgcaaagt cgcgggagcc gtcgaaaatc
    11401 tgctggcaac ccttgaatgg gctggagtca agccggacga aggaccgcaa atcggcggcg
    11461 actttgggcc atatgtgcag tcagaacgac ttcagcttta tcgtgattat gctcaattat
    11521 tgctggaaaa ggggcatgct tattactgct tctgttcagc agaacgacta aaaaaattga
    11581 aagaatggca aattgcccag aaaataccac cgcgttacga taatcattgc cgcgatttgt
    11641 cagtagaaga aatccaacaa catctatctt ctggcgaaga gtacgtcatc cggatgaaaa
    11701 ttccagccgc tggtgctatt gaagttgacg atctcatccg gggcaaagtc acttttccgg
    11761 ttagtcagat tgatgaccag gttctgctta aatcagatgg ttatccaact taccatctag
    11821 caaatgttgt agatgatcac ttaatgcaaa tcagccatgt cattcgcggc gaagaatggt
    11881 tgccgagcac acccaagcat gttttattgt atcgctattt cggttgggaa ttgccgcaat
    11941 ttgcgcatct ttctttattg ctaaatccgg acaaaagtaa actttcgaag cggcaaggtg
    12001 atgttgcggt tgaggattac cggcgcaaag gctacctgcc ggaagctctg ataaatttcc
    12061 tggctttatt aggatggaac ccgggcgacg accgtgaaat ttttagtctc gacgaattaa
    12121 ttcgtgaatt ttccttggaa cgcgtcagtc atagcggggc gattttcaac atcgaaaagc
    12181 tcaattggat gaatggaatt tacatccgta atttgtcgtt ggaacgtctc tgggaatacg
    12241 ccaaaccata tttagaggaa tgtctccacc agccttgcgt ggatgagcgg gctcattggg
    12301 acgaagcccg attaaaggca gtgttaaact ctgttaagga cgggttgtcc aacttttccg
    12361 aaattcctca gaaaacggct ttttattttg cctcatcaat ttcttatgag gcgcctgaaa
    12421 ttcaggatta tttaaccaca ccggaatcca taacagtcct gaaaactctg atacctttga
    12481 tagattctgt tggaataaat cagacggacg ggaagaagcc agatttcaac agcgacagct
    12541 ttatagcggc tgtcaggaat gttcagaaag caactggtat taaagggaaa ccgctctgga
    12601 tgaccattag agtcgcccta accggcacca cgcaaggccc agaaattcac caaattgcca
    12661 gtataattgg tcgtgaaacc tgtatcaagc gcctcgaagc ggctttgaat tatgccgaaa
    12721 gccacctcaa ttaaaatcaa gtctaacgcc aaaattaatc ttaccttgcg catcaccggg
    12781 cgtcgtccag atggctatca caaccttagt accatttttc aggaaattga tttgcacgat
    12841 gtccttacct tcacaccggc taaaaccttt gaattcgcca caaatcattt agcactttct
    12901 aataaaacgg acaatttgtg tgccaaagca taccgaagaa tggaacagat tgctccacat
    12961 agtcaaccct ggcggattac tttagaaaaa aatatcccaa tcggcgccgg gctgggcgga
    13021 ggcagttcca atgcggcaac cgtcttgaaa tttttgaata aagcctggca aatcaatctg
    13081 agcgatgaca acctggctcg tattggatgt gcgattggca gtgatgtgcc gttttacatc
    13141 aaaggaaaaa ctcaaggcgc cagaggaatt ggcgaagtac tcgaaccgct aattctaatg
    13201 gaagattttg tcattttgtt ggttctgccg gaaataaata tttcgacagc ttgggcgtac
    13261 cggcagttca atcccaaaaa tgccaaaccg gcctacaact ttgccgaact tatcagtggt
    13321 gggcgggttg attgggtaaa attcgaaaac caattcgaag aaatcgtttt tccggtatat
    13381 cctcagttag caaaactgaa ggagcaaatt ctaaatacag gtgcccttta cgctgggtta
    13441 tcgggaagcg gcgcggctct gtttggaatt tttagaaatc gtacttacgc tttaaaagcc
    13501 gcccggcaac ttccggaatg gcgtacaatt atcaccaaac cggtcaa
//
LOCUS       DAOMJU010000049        13398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_4607_length_13398_cov_23.429813, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000049 DAOMJU010000000
VERSION     DAOMJU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4607_length_13398_cov_23.429813"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PLO58_06020"
     CDS             <1..920
                     /locus_tag="PLO58_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQO74380.1"
                     /translation="SLLEEYKQMIISKIQLARPLKIAMDCGNGAASLTAPEIFKKIGC
                     QVTELYCEVDGRFPNHHPDPTVPKNLKDLIAEVQKGGYDFGVAFDGDADRIGVVDDLG
                     QIIWADYLMILFLEEVARPGVPVIFDVKCSQALEEMIISYGAKPIMWKTGHSLIKQKM
                     KDENAPFAGEMSGHLFMGDEYFGYDDAVYVAARLARLIARGNEKISAKIARLPRYFST
                     PEIRLECKDDPTKFKIAEQAAAYFKSHYDCIDVDGVRIKFGDGWGLVRASNTQPVIVT
                     RFEAKTAERLDEIRDLVLNKLREFGEFRL"
     gene            1003..1974
                     /locus_tag="PLO58_06025"
     CDS             1003..1974
                     /locus_tag="PLO58_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQO74381.1"
                     /translation="MSVDFGTKILRLKEEIDRELSKIDLSGISSELVQPMRFSLNAKA
                     KRLRPILVLLVGENLGASRKNLMPAALAIEIMHTFTLVHDDIMDNDTIRRGQQTVHVK
                     WNVNTAILTGDALMALAFRVLMQTKSERLDQIGTHFSQAMLEICDGQAFDLAYEKYDE
                     IDLTDYLNMIARKTGRLLGLACQLAAIIADSSPKVVNNLYLFGLELGQAFQIQDDLLE
                     LTADQAEMGKSLGSDLTAGKKTFPIIMALNELPAGQRADLLNFLKANAGNRRAIVQQL
                     TKRGSIQQTEAMIARLFKNARERLHNMPDQLQSDLINLIELIAGRQS"
     gene            1976..2242
                     /locus_tag="PLO58_06030"
     CDS             1976..2242
                     /locus_tag="PLO58_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQO74382.1"
                     /translation="MVRQTVKILNRHGLHTRPATVLVTLCSRFKSDIFLIYKNVRVNA
                     KSILGVLVLAVEPNAEVTIEVDGPDEQEALAAIVELVANKFNTE"
     gene            2247..3977
                     /gene="ptsP"
                     /locus_tag="PLO58_06035"
     CDS             2247..3977
                     /gene="ptsP"
                     /locus_tag="PLO58_06035"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HQO74383.1"
                     /translation="MKILKGIRISPGVAIGSAYYVETGKVAIPHTYIDQSEVEAELEK
                     FHRATQRATEELNQVRDMVMKHLDEEHARLIDAQIMTLADVDLVQKITDMVRQEHRNV
                     IWAYDAAMEWYENALLNTMNQFQQERLIDLQDIKKRMFRHLSTEEQLDLPQIPERVIY
                     VGERISPSELIQLHNQKILGIITHIGGMDSHSGILARAFGIPYLSNVNEIDLIGKSHQ
                     VILDADREIVIVDAKPSVLKKYAVKLRELEIRRQRLNETIPPAITRDGIKIEILLNAG
                     FLSEVKNINLSLIDGIGLFRTEYLCLERDDLPNEEEQFRAYRQITMAMQGKPTTFRTF
                     DFGREKMMAILNMEMFQKDAVFDTWGGIRFCLDNPRILITQLRALLRASQFGPLKIMF
                     PLVSNSDEIFQVFEIYRQVQSDLEKEGHAFNSNIPLGAMVETKSILDELEKLTELLNF
                     FSVGTNDLALFLLGSKRADTISKNYYHPKIFQAINQIINVAQKNNFPVTVCGEMASDP
                     YAILGLLALEVRSFSMSVSALIDINDLIRMVNIQKISSLKKNLLTADSVQQVYKLLRS
                     SYKRITKNKM"
     gene            4042..4623
                     /locus_tag="PLO58_06040"
     CDS             4042..4623
                     /locus_tag="PLO58_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HQO74384.1"
                     /translation="MIKVALNKIIFYPPSKGYALLLKEVGGERQLPIIVGVFEAQSIA
                     LAIEEVKMPRPMTHDLFVNILHDLGAKILSVTVSDLIDGTFYAKIEISLANSKKNLYI
                     DARPSDAIALAIRMKAPIQVSEEVMQEAGQYLNSKRKDSPEKDDSLPEIQNLDHLFEL
                     QNNLQSAIEEENYELAAKLRDQINRLKQELNIN"
     gene            5036..>5225
                     /locus_tag="PLO58_06045"
     CDS             5036..>5225
                     /locus_tag="PLO58_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HQO74385.1"
                     /translation="MNRRNIVIIGAAGRDFHNFNVYYRGNEQFNVVAFTAAQIPNIDN
                     RKYPSELAGKLYPKGIPIF"
     assembly_gap    5226..5325
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5326..6549
                     /locus_tag="PLO58_06050"
     CDS             <5326..6549
                     /locus_tag="PLO58_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HQO74386.1"
                     /translation="KYPSELAGKLYPKGIPIFPEEELTYLIEKFDVDECVFSYSDVPY
                     QKVMALSALVNAAGANFTLLGPKATMLKSTKPVISVCATRTGCGKSQTARKVIEILMA
                     QGLKVVAVRHPMPYGDLVAQKVQRFATLDDLKKYHCTIEEMEEYEPHIVRGNVIYAGV
                     DYEAILRAAENDPNGCDLIIWDGGNNDFPFFKPDLAITVVDPHRPGNELSYYPGEVNL
                     RIADVIIINKIDTANPEGIQTVSQNIALVNPQATVIYAASPVRVDHPEIIRDKRVLVI
                     EDGPTLTHGEMQIGAGIIAARQYGARECVDPRPFVVGKLAETFQKYPKIGPLLPAMGY
                     SEDQIRDLEDTIARTDCDSVVIGTPIDLTRILKIKQPSARVYYELQEIGTPNLNEVLS
                     DFCQRIKLNIQTKQI"
     gene            6546..7487
                     /gene="arcC"
                     /locus_tag="PLO58_06055"
     CDS             6546..7487
                     /gene="arcC"
                     /locus_tag="PLO58_06055"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HQO74387.1"
                     /translation="MNRTAVVALGGNAISPNNEKDTIANQFKNTRKSLQSVMNLIRQN
                     YHLALTHGNGPQVGNALLRVELTIDKAPFLPLGICVADTEGGMGYMIEQSLQNALIKA
                     QIPREVVSIITQVIVAADDPSINNPTKFVGSWYSYNEIKELAARFGWQIKEDGQRGWR
                     RVVPSPIPRKIINSRAIKHLVDLGTIVIAAGGGGIPVYIMENGLYEGFDAVIDKDLAA
                     AVLAHDIAAQELYILTDIDSVKINFGKPNEESIDLIHIPELQRLLEEGQFPSGSMGPK
                     ISAAIAFIQSGGERVIITSLEKAIESLHGDSGTTIIA"
     gene            7499..7915
                     /gene="ndk"
                     /locus_tag="PLO58_06060"
     CDS             7499..7915
                     /gene="ndk"
                     /locus_tag="PLO58_06060"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQO74388.1"
                     /translation="MSMTLAILKPDCVKRKLIGEVISFIEKEGFRIVCLRMIHLQPQE
                     AAEFYSIHRGKDYFEGLMNFMTSGPSVLMVLERENAVEYFRKVIGATDPQKAAEGTLR
                     RKYAENVRYNVVHGSDSDENAKKEIAFFFSTMEIIK"
     gene            7939..8703
                     /locus_tag="PLO58_06065"
     CDS             7939..8703
                     /locus_tag="PLO58_06065"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQO74389.1"
                     /translation="MKKLSLTLTILLMLLSNCSKKPVVVTGVVGNPINLVPEIKKSED
                     TTGCTFKWGFIAKPAESIMDVLSFIPDSRSFSVSFIPDVPGEYELQFLSQDSNGEEKF
                     KQIIKCSVLPDTAQSPSTDTVAGQMPPRDLSAPPPQYAQSYPGGENETTYSAMTQSQP
                     IKTGRNIQKIPGKYTIQLGAYKNYDKAEAELKKLRAHNLDTYIQKAYFKETNETWYRI
                     RTGTFDSYAEAKKVLNQLKARFPKENLWIDYVREDQ"
     gene            8724..9908
                     /locus_tag="PLO58_06070"
     CDS             8724..9908
                     /locus_tag="PLO58_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74390.1"
                     /translation="MKKLKFDVRDIFLTPRLALSGKKIWVHMVGLAAGWVIYTILAYV
                     ALLVNGQSLAEIWRMYHFFPILCCTTCQLPWYSWLIYGVGVVAWIIIFLLASVAVARI
                     TYKQLKGDEFFSSGDSFKYVKKHGVAAVMGPVSILMIIAFFVILAIIAAWLAKWPVLD
                     IIFLGIPYLLYFVVATFVVYTAVVFVVSLVLAPAIVGTAEEDTMETVFQSYSTLWAQP
                     WRLVLYEGIVGGLTVVATFIYGWALILGYRFFNFVFSASWLMDGKMSRIIEQASSYLF
                     GINSPLTPWISQFFNVYTPASLGIVKPLYLGTTEIITVVLITIALFIIAGTVVAYALS
                     NLSVGQALIYIILRKKKDDEDLIERKDEDELKEEEKSEEPASEETKAPESETPSSDEK
                     TE"
     gene            complement(10089..10616)
                     /locus_tag="PLO58_06075"
     CDS             complement(10089..10616)
                     /locus_tag="PLO58_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQO74391.1"
                     /translation="MNTKTKAQYVNISILSKYEIFNSLSEKNIEHFCEVIEVLEYESG
                     DTIIKEGEVGDSLLLLLDGTVEISQALTLKTDMSGIDTREKSLIKLSSTARPFFGEMS
                     LFSDDDKRTATVKASTKCLVGRISRVDFFQICNSNPTIGNIVMQNIARVLTERLKQAN
                     QNVLKLTTALSLLIE"
     gene            complement(10613..11755)
                     /locus_tag="PLO58_06080"
     CDS             complement(10613..11755)
                     /locus_tag="PLO58_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQO74392.1"
                     /translation="MKFSETFKSVVTRSTLKVFLVFLILIFSAAIFVFFFEKQNNPDE
                     YHNLWDAVWWVFVTIFTVGYGDIKPITPGGRLVAIFIMLVGVSMVSTITATISSIFVT
                     RKIREAQGLEAINYENHLIICGWNNRAESLINTILQLSGKKDMKLVLINELAENEINT
                     ILEKYSQFKIKFVYGDYSHPAPLERANIKRARTVLLLPNLHRYSPADADEKTILATLN
                     IKSSFPKVRVIAFIMNPENEAHVKRAKADEIVVSDKYADYFLAINILQPELPKVYDQI
                     LNPRMQNKIVVEAIPASFMNKPYRELFDYYHTQYKTLLLGLISEVESIGFSDFLSADT
                     SQLDAFIERKLRESGKSFGEENKVFVKLNPGDDYLIQKNEKAIVLR"
     gene            complement(11833..13383)
                     /gene="gpmI"
                     /locus_tag="PLO58_06085"
     CDS             complement(11833..13383)
                     /gene="gpmI"
                     /locus_tag="PLO58_06085"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQO74393.1"
                     /translation="MRPVAIIIRDGWGYNENPRGNAVLAAKTPNIDSYKKKYPWTLLK
                     CSGEAVGLPEGYQGSSEVGHLNMGAGRIVIQELKRIDDGLSDGSLFESPKWKNLVANW
                     KANNSAFHLFGLLQDEGVHAHQEHLFKIMRRARQEFPEGEIIVHPFLDGRDTPPRSSL
                     EYIAKLNRVLAEVGHAKIGTIMGRYYGMDRSKNWNLTDTAYHCIVLAEGRPAPSAEAA
                     IRESYANDKTPDNVEMFDEYIPPYVIGGYTGMRDGDVVLHTNYRQDRAIQLTRAFVDP
                     DYPGKLKAKPKVTYVGFTQYYDEFTEYLLGSMSSGGGMKNLLGEVISNAGLRQLRIAE
                     TQKFRHVTSFFNGKSTTPYPLEDQVEIKGRFDPATFASHPEMEAYNVTDELLRRLENN
                     PYTLIVVNYANGDMVGHTGVFEAAKKAIEIVDDNVGKIVRRLLELDAHILITADHGNS
                     EQMIDYETGMVKTSHTLFPVELIYVARDLAGKSLKSGGKLADIAPTALKLLGLEIPKD
                     MTADCLIS"
BASE COUNT         3847 a         2865 c         2895 g         3691 t
ORIGIN      
        1 aatctttgct ggaagaatac aaacaaatga tcatatccaa aatccaatta gctcgtcctt
       61 tgaaaattgc tatggattgc ggtaacggcg ccgcatctct gactgctccg gaaatcttta
      121 aaaaaattgg gtgtcaggtt acagaactct actgtgaggt tgatggtaga ttcccaaatc
      181 atcatcctga tccgacggtt cccaaaaatt tgaaagactt aatcgcagaa gtacagaaag
      241 gcggctacga tttcggggtg gcattcgacg gtgacgccga ccgcatcgga gtggttgatg
      301 atctcgggca aatcatttgg gctgattacc ttatgattct gttccttgaa gaagttgccc
      361 gaccgggtgt gccggttatt ttcgatgtca aatgttcaca ggcactggaa gaaatgataa
      421 tatcttacgg ggcaaaaccg attatgtgga aaactggcca ttctcttatt aaacagaaaa
      481 tgaaggacga aaatgcacct ttcgctggtg aaatgagcgg acatttattt atgggcgacg
      541 aatacttcgg ctatgacgat gctgtctatg tggcggcgcg acttgcccgt ttgatcgcgc
      601 ggggcaatga gaaaatttcc gctaaaatcg cgcgcttacc ccgttatttc agcacaccgg
      661 aaattcgtct cgaatgtaag gacgacccca caaaattcaa gatcgccgag caggcggccg
      721 cctatttcaa gtctcattac gactgcatag atgttgacgg cgtgcgaatc aaattcggcg
      781 atggctgggg attggtacgg gcttccaaca ctcagccggt gattgtgact cgctttgagg
      841 cgaaaaccgc cgagcgcttg gatgaaatcc gtgatttggt gcttaataaa ttgcgcgaat
      901 tcggcgaatt tagactttga taaagtttta cacgaatagg gtctaatttt ttttccttga
      961 aaattattgt aaacttggaa gtgtattttc ggatgaggat ttatgtcagt tgacttcggc
     1021 actaaaatct tacgactcaa agaagaaatc gatcgcgaac taagtaaaat tgatttatcg
     1081 ggaatctcga gtgaactcgt ccaaccgatg cgctttagcc tgaatgccaa agccaaacgc
     1141 ttgcgaccga ttctggtgtt attagtcggt gaaaatttag gcgccagccg gaaaaatctt
     1201 atgccggccg ctctggcgat tgaaattatg catactttca ccctagtgca tgacgatatt
     1261 atggacaatg acaccatccg acgcggtcag cagaccgttc atgtgaaatg gaatgtcaat
     1321 actgctatct tgaccggtga tgctttgatg gcacttgctt ttcgcgttct aatgcaaacc
     1381 aaatccgagc gtctcgatca gattggcact cacttcagtc aagctatgct ggaaatttgc
     1441 gatggccagg cattcgacct ggcttatgaa aaatatgatg agattgatct cactgattat
     1501 ctgaatatga ttgctcgcaa aaccggccga cttctgggat tagcctgtca gctggcggcg
     1561 atcatcgccg attccagtcc gaaggtggta aataatctct atctttttgg cttggaattg
     1621 ggacaagcct ttcagattca ggacgattta ttggagctga cagctgacca agctgaaatg
     1681 ggtaaaagtc tcgggagcga tctcaccgca gggaaaaaaa cttttcctat tattatggcg
     1741 ctgaatgaat tgccagccgg tcagcgcgcc gatttactta atttcctcaa agccaatgcc
     1801 ggtaatcggc gggctatcgt tcaacaattg accaaacgcg gttcaattca acagacggaa
     1861 gctatgatcg cccggctttt taaaaatgcc cgcgaaagat tgcacaatat gcctgatcaa
     1921 ttacaaagcg acttgattaa tttaatagaa ttaatagcgg gacgtcaatc ctaagatggt
     1981 cagacaaacc gttaaaattc taaatcggca tggtctgcat actcgaccgg ctaccgttct
     2041 ggttaccctt tgttcccgtt tcaaatccga tatatttcta atctataaaa atgtgcgcgt
     2101 caatgctaag agcattctcg gggtactggt tttagcggtc gagccgaacg ccgaagtcac
     2161 tatcgaggtg gatggacctg acgaacagga agccttagcc gccatagttg aactggtagc
     2221 caataaattc aatacggaat aaaaaagtga agattcttaa gggcatccga atctctcccg
     2281 gcgttgctat tggctcggca tactacgttg aaaccggaaa agtcgcgatt ccccatactt
     2341 atatagatca atctgaagtc gaagccgagc tggaaaaatt ccaccgagct actcaacgtg
     2401 caacggagga attaaatcag gtgcgggata tggtaatgaa gcacctcgat gaagaacacg
     2461 cccggttgat tgacgctcaa ataatgacat tagctgatgt agacttagtc caaaaaatca
     2521 ccgatatggt gcggcaggag catcgtaatg tgatctgggc ttatgatgcc gcaatggagt
     2581 ggtatgagaa cgctttgtta aatactatga accaatttca gcaagagcgt ctgattgatc
     2641 tccaggatat caaaaaacgt atgtttcgtc atctttcgac ggaggaacaa cttgacctcc
     2701 ctcagatacc cgaaagagta atatacgtcg gtgagcggat ttcaccatct gaactaattc
     2761 aattacataa tcagaaaata ctgggaatca tcactcatat cggcgggatg gattcacatt
     2821 cgggaattct agcccgagca tttggaattc cctacttatc caatgtcaat gagattgacc
     2881 taatcggcaa aagtcaccaa gttattctgg atgccgaccg tgaaatagtt attgtcgatg
     2941 ccaaaccgtc cgtgcttaag aaatacgcag taaaacttcg ggaattagaa atacgtcgtc
     3001 agcgtctaaa tgaaaccata cccccagcca tcacccgtga cggaatcaaa atcgaaattc
     3061 tgctgaacgc cggattcctt tctgaagtta aaaatatcaa tctgagcctg attgacggaa
     3121 taggtctatt ccgaacggaa tatctatgtc ttgaacgcga cgatctccct aatgaggaag
     3181 aacaatttag ggcttatcgc cagataacaa tggctatgca gggcaaacca actacctttc
     3241 gcactttcga ttttggtcgc gagaaaatga tggctattct caatatggaa atgtttcaaa
     3301 aagatgcggt ctttgacacc tggggtggaa ttcgtttctg tttagacaac ccgcgaattt
     3361 taatcaccca actgcgggct ttgctccgtg ccagccagtt cggcccgctg aaaattatgt
     3421 ttccgctggt ttccaattcc gatgaaatct ttcaggtttt tgaaatctac cggcaagttc
     3481 aatccgattt agaaaaagaa ggacatgcct tcaatagcaa tattcctctc ggtgctatgg
     3541 tcgaaactaa aagtattttg gacgaactgg agaaactaac tgaattgctg aacttcttta
     3601 gcgtcggcac caatgacctg gcactttttc tactcggttc aaaacgagcc gataccatct
     3661 ccaagaacta ctatcacccc aaaattttcc aagccattaa tcaaataata aatgttgctc
     3721 aaaaaaataa ctttccagtc acagtttgtg gtgaaatggc ttccgatccc tacgctatcc
     3781 tcggtttact ggctttggag gtacgctctt tcagtatgag cgtcagcgcc ttgattgaca
     3841 ttaatgactt aatccgaatg gtaaatattc agaagatcag ttcactaaag aaaaatctac
     3901 tcaccgccga tagcgttcag caagtttata aacttttacg aagctcctat aagcgtatta
     3961 caaaaaataa gatgtaatag gggaaactta ttttaatact tgactctgct tgacagttat
     4021 gatatattca gacataagaa tatgatcaaa gtcgcactca ataagatcat tttttacccg
     4081 cctagcaaag gctatgccct ccttttaaaa gaagtcggcg gcgaacgtca gctcccgatt
     4141 atagtaggtg tttttgaagc ccagtccata gcactcgcta tcgaggaagt gaaaatgccg
     4201 cgtccaatga ctcatgattt attcgttaat attttacacg atttaggcgc taaaatatta
     4261 tcagtgactg tcagtgattt gatagatgga actttttacg ccaagattga aatcagcctg
     4321 gctaatagca agaaaaattt atatatagac gctcgaccga gcgacgctat tgccttggca
     4381 attcgcatga aagcacctat tcaagtaagt gaggaagtaa tgcaggaagc tggacaatat
     4441 ctgaactcca aacgtaaaga ttctcctgaa aaagacgatt cccttccaga aatacagaat
     4501 ctggatcatc tcttcgaatt gcagaataat ctccaaagcg ccatcgaaga agaaaattac
     4561 gaattagccg ctaagctacg cgatcagatc aatcgcctta aacaagaatt aaacatcaat
     4621 taaaaatcaa taaaagaatg tgatttacta cctttcttta taataaggaa gcagttgcag
     4681 catttattta ctcaaaaatc cgttaaaaaa tattagaaaa aattccaaat tcccatttta
     4741 ttggaaattc tgagaattat tttttaagca attttaatct ggcaggcctt taacttgacg
     4801 aagcaagtcg ccgccccttg aaattcgaca taaatttacc taactttatt aagtgaaaat
     4861 cattttgctc cctataactc acgatgtatc tgccgccggc tcggtcgaaa gaacagatag
     4921 ttataaaaat gccattatac tctggaccta tcattttggc tataaataaa tttcctgcgc
     4981 cttcactctc agtaaatttt tttaaaacca ataaatcaat gaaaggaatt aaatgatgaa
     5041 tcgcaggaat atcgtcatta tcggcgcggc tggtcgcgat tttcataatt tcaatgttta
     5101 ctatcgtggc aatgaacaat ttaatgtcgt cgccttcacg gcggctcaaa ttccaaatat
     5161 tgacaaccgc aaatatccgt ccgaattggc tggaaaactt tatccaaagg gcattccaat
     5221 tttccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaata tccgtccgaa
     5341 ttggctggaa aactttatcc aaagggcatt ccaattttcc ctgaggaaga gctgacctac
     5401 ctaattgaaa aattcgacgt tgacgaatgc gtcttttcgt atagtgatgt gccctatcag
     5461 aaagttatgg cgctttcagc gctcgtcaat gcggcgggag cgaatttcac cttactcgga
     5521 ccaaaagcaa ccatgctgaa aagcaccaaa ccggtgattt cagtctgtgc tactcgcacc
     5581 ggttgtggaa aaagtcagac agcacgcaag gtgattgaaa ttttaatggc gcagggtttg
     5641 aaggtggtag ccgtgcgtca tcctatgccc tacggtgatc tggttgccca aaaagtccag
     5701 cgctttgcca ctttagacga cttaaaaaag taccattgca ccatcgaaga aatggaagaa
     5761 tatgaacccc acatcgtgcg gggtaatgtc atctacgccg gagttgatta cgaggcgatt
     5821 ttgcgagcgg cggaaaacga tcccaacgga tgcgacctga tcatctggga cggcggcaat
     5881 aacgattttc ctttttttaa acccgacctg gcaatcacgg tcgttgatcc ccaccgaccc
     5941 ggtaatgaac ttagttatta tcccggtgaa gtcaacttac gcatcgctga cgttatcatc
     6001 atcaataaaa ttgataccgc taatccggaa gggattcaaa ccgtgagcca aaatatagcc
     6061 ctagttaacc ctcaggcaac ggttatttat gcggcttcgc ccgttagagt tgaccatcct
     6121 gaaattatcc gcgataaacg agtgttagta atcgaagacg gtccgacgtt aacccatggg
     6181 gaaatgcaaa tcggcgctgg cataatagcg gctcgtcaat acggcgcccg ggaatgtgtt
     6241 gacccccgtc ccttcgttgt gggaaaactg gctgagacat tccaaaaata tccgaaaatc
     6301 ggtcctcttt taccggctat gggttatagt gaagaccaaa ttcgcgatct ggaagatacg
     6361 attgcgcgga ctgattgcga ttcagttgtt atcggaacac caattgatct gactcgcatt
     6421 cttaaaatca aacagccttc tgctcgggtt tactacgaat tacaggaaat cggcactccc
     6481 aatttaaacg aagtattatc cgatttctgt caaagaatta aacttaatat tcaaacaaag
     6541 cagatatgaa tcggaccgcg gttgtagcat taggcggcaa tgccatctca cctaataatg
     6601 agaaggacac aattgctaac cagtttaaaa acacgcgcaa aagtctgcaa tcggtgatga
     6661 atctaattcg ccagaattat catctggcat tgactcacgg caatgggcca caggtcggca
     6721 atgcccttct gcgggtcgaa ctaacgattg acaaagcccc attcttaccg ctgggaattt
     6781 gcgtagcgga taccgaaggc ggtatgggct atatgatcga acaatccctc cagaatgccc
     6841 ttataaaggc gcagataccc cgggaggtgg tttcaattat tacccaggtg atcgtcgcag
     6901 cagatgatcc ttcaattaat aacccgacta aattcgtcgg aagttggtat tcctataatg
     6961 aaatcaaaga actggcggca cgcttcggct ggcaaattaa agaggacggt caacgcggat
     7021 ggcgccgagt agtgccttcg ccgatccccc gcaaaattat taacagccgt gctattaaac
     7081 atttggttga tttaggcacc attgtgattg cggcaggagg aggtggcatt ccggtttata
     7141 taatggaaaa tggactatac gaaggttttg atgccgttat agataaagac ctggcggcgg
     7201 ctgtcttggc tcacgatatc gcggctcagg agctgtatat tctgaccgat attgatagtg
     7261 tcaaaattaa ttttggcaaa cccaatgaag aatctattga tttgatccat atccccgaat
     7321 tgcaacgctt actggaagag gggcaatttc cgtcgggctc gatgggacct aaaatcagcg
     7381 ccgctatcgc ctttatccaa tctggcggtg aaagagtaat catcacctcc ctcgaaaaag
     7441 caattgaaag cctgcatggt gatagcggta caaccatcat cgcatagaaa ggaaaaatat
     7501 gtcaatgaca cttgctattc ttaaaccgga ttgtgttaaa cgtaaattga tcggcgaggt
     7561 gattagtttc atcgaaaaag aaggctttag gattgtttgc ctgcggatga ttcatcttca
     7621 accgcaagaa gcggctgaat tctatagtat tcaccgcggg aaggactatt tcgaaggttt
     7681 aatgaatttt atgacctccg ggccatctgt tttaatggtt cttgagcggg aaaatgctgt
     7741 ggaatatttt cgcaaagtga ttggagcaac cgatccacaa aaagccgctg aaggaacctt
     7801 acgtcgaaaa tatgccgaaa atgtacgata taacgttgtc cacggctcag attctgatga
     7861 aaatgcaaag aaagaaattg catttttctt ttctacgatg gaaattatca aatgattaac
     7921 atgcggaggg ccagatttat gaaaaaacta tctttgacct taacgattct actgatgctt
     7981 ctgagcaatt gtagcaaaaa accggtggtc gtaacaggtg tcgtcggcaa tcccatcaat
     8041 ttagttccgg agatcaaaaa gtcggaagac actactggat gcacttttaa atggggtttt
     8101 attgccaaac cggctgagag tattatggat gttctctcgt ttatcccgga tagtcgcagt
     8161 ttttcagttt cttttatacc ggatgttccc ggcgagtatg aattgcagtt tctcagtcag
     8221 gacagcaacg gcgaagaaaa attcaaacaa attataaagt gtagcgtatt gcccgatact
     8281 gctcaatccc ctagtacaga caccgttgct ggtcagatgc caccgcgaga tttgtcagca
     8341 cctccccctc aatacgctca atcctatccg ggcggggaaa acgaaacgac ctattcagct
     8401 atgacccaat cccaaccgat caaaaccggg agaaacattc agaaaattcc tgggaaatat
     8461 acaatccagc tgggcgctta taaaaactat gacaaggctg aggccgaatt aaaaaaatta
     8521 agagcacata atctggatac ttatatacaa aaggcctatt ttaaggaaac taacgaaact
     8581 tggtatcgca tccgcaccgg aacctttgat tcttacgctg aagccaaaaa agttttgaac
     8641 cagctaaaag cgcgttttcc caaagagaat ctctggatag attatgttcg tgaagatcaa
     8701 taaacaattg taggaggtta taaatgaaaa aactgaaatt tgatgtaagg gatattttct
     8761 tgactcctcg tttggcactg agtggcaaga aaatctgggt tcatatggtc ggattagctg
     8821 ctggctgggt aatctacacc atacttgcat atgtagcttt gctagtcaat ggccaaagcc
     8881 tggcagaaat ctggaggatg tatcactttt tcccaattct atgctgtacc acctgtcaat
     8941 taccctggta tagttggtta atttacggag tgggggtggt cgcctggata ataattttct
     9001 tactggcatc tgtagctgta gcacgaatta cttacaaaca attaaaaggt gatgagtttt
     9061 tctcatcagg cgacagtttc aagtatgtca aaaaacacgg tgtcgccgct gtaatgggtc
     9121 cggtttctat tttaatgata attgcttttt tcgtaattct ggcgataata gccgcctggc
     9181 ttgccaaatg gcctgtcctt gatattattt ttctggggat accgtatctg ctctattttg
     9241 tcgttgcaac ttttgtcgta tatactgcgg tggtctttgt tgtgtcgctg gttctggctc
     9301 cggcgatcgt cggtacagct gaagaagaca ctatggagac cgtttttcaa agttactcca
     9361 cactttgggc acagccctgg cgattggttt tatacgaagg gattgtcgga ggactaacgg
     9421 tagtggctac ttttatttac ggctgggctt taatccttgg ctatcgattt tttaacttcg
     9481 tatttagcgc tagctggcta atggatggta aaatgtcccg cataatcgaa caggcttcct
     9541 cttacctgtt cggcatcaat tctccattaa ctccctggat tagccaattt tttaatgttt
     9601 atactccggc ttcgctaggt atagtaaaac cgctttatct tggaacaacc gaaattataa
     9661 ccgtggtttt gataacgatc gccttgttta taattgccgg aacggtagtt gcttatgccc
     9721 tttctaacct cagtgttggg caagccctta tttacattat actccgtaag aaaaaagatg
     9781 atgaggatct catagaacgg aaagatgaag acgagttaaa agaggaagaa aaatccgagg
     9841 agcctgcgag tgaggaaaca aaagctccgg aatctgaaac tccttcttca gacgaaaaga
     9901 cagagtagca aagtcttttc gttggttttg ataaaaaggg cgtccgaatt ctgacgccct
     9961 ttttgtttgg tttatatagc ctgatatcgg gatagatttt ctataaaagt ttcatcggaa
    10021 ccccaaccga gcaaatagtg gatactttgc caattttaaa aattttgtca agagaaaaaa
    10081 tacattcact attcgattag cagactgagc gccgtggtca gtttcagaac gttctgattg
    10141 gcttgcttca agcgctcggt caagacgcgg gcaatatttt gcattacaat attgccgatc
    10201 gtcggatttg agttgcatat ctggaagaaa tctactctgc taatgcgacc gaccaggcat
    10261 ttagtgctgg ctttgacagt ggctgtgcgt ttgtcgtcat cgctaaagag actcatctct
    10321 ccgaaaaatg ggcgggcggt gctggacaat tttatcaagc ttttttcacg agtatctatc
    10381 ccggacatat cggttttcaa agtcaaagct tgcgaaatct cgacagtacc atccagaagt
    10441 agaagaagtg aatcgccgac ctcgccttcc ttgatgatcg tatcgccgga ttcgtattcg
    10501 agaacctcaa ttacctcgca aaaatgctca atgttttttt cggaaagact attgaaaatc
    10561 tcatattttg ataaaatgga aatatttaca tattgggctt tagtcttggt attcatcgga
    10621 gaacaatcgc cttttcattt ttttggatta gataatcgtc tcctggattt agtttgacaa
    10681 agaccttatt ttcttcgccg aaactcttgc cggattcacg taatttacgc tcaatgaaag
    10741 cgtcgagttg agaagtatcc gccgaaagga aatcggaaaa tccgattgac tcgacttcag
    10801 aaattaagcc aagcaagaga gtcttatatt gagtatggta gtaatcgaat aattcccgat
    10861 aaggtttatt cataaaactg gctggaatag cttcgaccac aattttattc tgcatgcgcg
    10921 gatttaaaat ctggtcgtaa accttcggta attccggttg taaaatatta atcgccagaa
    10981 aataatcagc gtatttatca ctaactacga tctcgtcggc tttagcccgt ttgacatgcg
    11041 cttcattttc gggattcata atgaaagcaa tgactctgac cttcggaaac gacgacttta
    11101 tattcaaggt tgccagaata gttttttcat cggcatcggc tggcgaatag cgatggagat
    11161 tgggtagaag caaaactgtc ctggcgcgct tgatgttcgc ccgttcgagg ggtgccggat
    11221 gggagtaatc tccatagaca aatttgattt taaattggga atatttctcg aggatcgtgt
    11281 taatttcatt ttcggccagt tcattgatta atactaattt catatccttt ttgccggaca
    11341 attgtaaaat agtattgatt aatgattcgg cgcgattatt ccagccgcaa ataatgagat
    11401 gattctcata attaatagct tccaaacctt gtgcctccct aatttttcgt gttacaaaaa
    11461 ttgaagagat cgtcgcggtg atggtcgaaa ccatcgaaac gcctactagc attatgaaaa
    11521 ttgcgacaag ccttcctcct ggagtaattg gcttaatgtc gccgtaacca accgtgaaaa
    11581 ttgttacaaa aacccaccaa accgcgtccc agagattgtg atattcatca gggttatttt
    11641 gtttttcgaa gaaaaatacg aaaatcgccg ctgaaaaaat taagattaga aaaaccaaaa
    11701 atacttttag ggttgatcgg gtaacgacag atttgaaggt ttcagaaaat ttcacattat
    11761 ctcagattaa caattctaac tgccaaaatt tcgttcgcta tcctttagca tcagcaatct
    11821 tgaactttgt agttagctaa tcaaacaatc agccgtcata tctttcggaa tttccagacc
    11881 gagtaatttc agagcagtag gcgcaatgtc cgccaattta ccgccagact tgagtgattt
    11941 tccggctaaa tcgcgggcaa cgtaaatcaa ttcaaccgga aagagagtat ggctggtctt
    12001 aaccataccg gtttcgtagt ctatcatctg ttcggagtta ccgtgatcag cggtaattag
    12061 aatatgcgcg tccagctcta agagacgtcg gacaatttta ccgacattat catcaacaat
    12121 ctcaatggct tttttagccg cctcgaaaac tccagtatgt ccgaccatat caccattggc
    12181 ataattaacg acaatcaaag tatagggatt attttccaag cgtcgcagta attcgtcggt
    12241 tacgttgtaa gcttccattt cagggtggct ggcaaaagtt gccggatcga agcgaccttt
    12301 gatttccacc tggtcttcca gcggataggg tgtcgtggac ttcccgttga aaaagctggt
    12361 gacgtgccga aatttttggg tttcggcaat acgtaattga cgtaaaccgg cgttggaaat
    12421 cacctctccc aagagatttt tcattccgcc accggaactc attgacccga gcaggtactc
    12481 ggtaaattca tcataatatt gagtaaaacc gacataagtg acttttggct tggcttttaa
    12541 tttaccggga tagtccggat caacgaatgc tcgcgtaagc tgaatggcgc gatcttgccg
    12601 gtaattagtg tgcagtacaa catcaccgtc tcgcattccg gtataaccgc cgataacata
    12661 cggtggaata tattcatcga acatttcgac attatcgggt gtcttgtcat tggcatagga
    12721 ttccctgata gcggcttcgg cactcggggc cggtcgccct tccgcaagaa cgatgcagtg
    12781 ataggcagtg tcggttaaat tccaattttt ggagcggtcc ataccgtagt aacgccccat
    12841 aatcgtacca attttcgcat gcccgacttc tgctaaaacg cgattaagct tggcaatata
    12901 ttccagcgaa ctgcgcggcg gtgtatcacg accatcaaga aaagggtgca cgataatttc
    12961 gccttcagga aattcctgcc gcgcccgacg cataattttg aaaagatgtt cctggtgagc
    13021 gtgaacgcct tcatcctgaa gaagcccaaa aagatgaaag gccgaattgt tggctttcca
    13081 gttggctacc agatttttcc atttgggcga ttcaaataac gacccatcgg aaagtccgtc
    13141 gtcaatgcgt ttaagttcct gaattacaat ccgcccagcc cccatattca ggtgaccgac
    13201 ttcgctactc ccttgataac cctcgggcaa cccgactgct tcgcccgaac attttagcag
    13261 tgtccaggga tacttttttt tataagaatc aatattgggc gttttagccg ccaagacggc
    13321 attgcctctc gggttttcat tgtaacccca accatcacga ataataattg ctaccggtcg
    13381 catttttacc tctgattt
//
LOCUS       DAOMJU010000050        13371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_4625_length_13371_cov_24.977170, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000050 DAOMJU010000000
VERSION     DAOMJU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13371
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4625_length_13371_cov_24.977170"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PLO58_06090"
     CDS             complement(<1..1292)
                     /locus_tag="PLO58_06090"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HQO74394.1"
                     /translation="MSSSDAADLRPILSYLYSLNGRGIKPGLERIQKLLDAIGNPQNA
                     YKVIHIAGTNGKGSTCAIMANVLQTAGYRVGLYTSPHLLNFNERIRINGQKIPDAEIA
                     LFTERWRKLIDELNCSFFEATTAMALDYFKKNKVDYAVLETGMGGRFDATNVTLPEVA
                     VITPIGRDHQEFLGRRLAQIAYEKAGIVKRGIPCIVARQTPRVLNFLSKEINASGGDF
                     YYAPEQCQITNIQRFPERQVLSLIVNNLKIDTVKFPLIGDHQLINLQAAVSALMRVKD
                     LELSAEVLRQGITGVIWPGRLHILSHKPLIYFDVGHNQHGIRQVVKALKQIFPAQPVH
                     LVLALGSVKKYDRIGKIIQPLGGDIYLTEIPDHPSVSAEILADVIRKQIPSRLIYVDN
                     KLERLLNFLEIQIKSDEILLIAGSHYLAPQVLPFYGVS"
     gene            complement(1292..2128)
                     /gene="murI"
                     /locus_tag="PLO58_06095"
     CDS             complement(1292..2128)
                     /gene="murI"
                     /locus_tag="PLO58_06095"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQO74395.1"
                     /translation="MTNQWLKVSNKRNLPIGVFDSGLGGLTVVKQLFRQLPQEQIYYF
                     GDTARVPYGNKSVETVQKFAIQIARFLAERSVKMIVVACNTASSVALELLQDQFDLPI
                     IGVIEPGARSALAISPTKKIGVIGTTGTINSGKYVEVLTALDKSVQVFSQACPLFVPL
                     VEEGWGESPVTEIVAREYLQNLVPKIDTLILGCTHYPIIKNVIQRIIDSNIQIIDTAI
                     ETANQVRLILNQQGLLNDLERTPEHRFYVSDLPQKFEEIAMRFLGEPIANVERINLAD
                     CC"
     gene            complement(2100..>3400)
                     /locus_tag="PLO58_06100"
     CDS             complement(2100..>3400)
                     /locus_tag="PLO58_06100"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQO74396.1"
                     /translation="ILQMSQPAILVNRDEIFVPMYSFLDLMKRYAIPKLQYYLDKRSP
                     IYELVELPGATPIPSAPTVTTFGTKPGIIYDIQFEERGNGLTMKISTNATYESSDFSS
                     FFRGDEWFYLTILGATCDSAALSRRDPIPSVAIVEAIPVGNSVQITLKLKQKYDRANA
                     YYDARSGKILVTLNVPLTSATLKKIEEVKNTWIVDTIVIDAGHGGKDSGTPGRWGYKH
                     EKDIVLDIALRLGKLLEQRNDLKVVYTRKNDEFIPLWKRTRIANDSAGKLFISLHINA
                     LPSGTRSSAEGIELYLQNPTIRTQESIEVARLENEVIKLETSEDKAKYKDYDNPSHIL
                     ANMVYNTYLHDSEKFAEILSRNISEYVPQKNRGVKQANFYVLVGASMPNLLCELGYND
                     NRNDAKKLNDPAHRQKIALALYKSVIEFKEYCDKSVAQSK"
     assembly_gap    3401..3500
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3501..5363)
                     /locus_tag="PLO58_06105"
     CDS             complement(3501..5363)
                     /locus_tag="PLO58_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74397.1"
                     /translation="MRLKFLIGLLFSYLIITIAEAPAFGQNDLSADILDNYFTDNALD
                     IDELQELIYAYRDNPLVWETCRIKDLRALPLNDYIISQLIELKRHYPKSLNLKALVKD
                     TIFTTAEIEAISYFIDFSVKRTKPSQIVNFISLKKQEYTSLQKSLQRARCYSPQGWFT
                     GIIVENDSDEPILGDYWNISLRSPKYFNSLEILGGAYRLQWGQGLLFANNLMSGRSTD
                     ATGNLKTSRSGVSNYLGSDENRYLFGSAAQYTIGNFKLTPFLSHHRLDATIDTNGTVK
                     NLRSDGLHISSSQLRAKDALLETLGGASLLGEWDQGSAGILVYNANYSTGLFALNGHR
                     KVSGLSFLHNYEISDWTISGEWVVQETGKKGIIQNLHLSRDHVSFCAGWRYIEPDFFA
                     PLGNPFRKFSSLSGNETGLYSALKIKLPARWRFTGYVDFFRELKNSEPGVAIRNGRES
                     LCGFSRQARNNDLIEAWYKVNRYYHTNLPQQPRDYQLKLHLRHSFSSSFAGEIRTTFR
                     WNDSIPFTEGSQALSVVGKVSISKYTRVTAGNTHYFTGSSDLNIYFYEPGLPFRFNMT
                     LLTGTGQRYFLIISRRIGSNCEIAGAVYGRSNVSIHTNDNSFDFSGDLQLTFDL"
     gene            complement(5345..6139)
                     /locus_tag="PLO58_06110"
     CDS             complement(5345..6139)
                     /locus_tag="PLO58_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74398.1"
                     /translation="MEKRFLFCLLLCIGTAQAAFEIHQASPIIIAGGGTISVFPDGSN
                     PASLVEKKGLSTCCNFSNLYGIKNLQTWDIGLFYNHHSKTGLALRLNTLGNLVYQEKT
                     IGFSLARKISSLISLGATVNYYDLTISNYPRTGAIGLNCGLKLFPDSTLGFAILFENL
                     NAPKICDRQEPLPQVFAFGWQWKPIRRGEISGEIFKDTRFPFTWRSGIRFEVFRGCNL
                     LAGVQFNPDRLSGGLELAWYKFQIAVAFLHHPTLPYTVYYQCGLNF"
     gene            complement(6260..6607)
                     /locus_tag="PLO58_06115"
     CDS             complement(6260..6607)
                     /locus_tag="PLO58_06115"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQO74399.1"
                     /translation="MRKSRPKSIKSKDGSGYLHKLYWILGILCAITLFIIFFVIGDYG
                     LYQIYLLNKQKEQIENHLVELQYTQDSLLIEKTRLESDLQYIEKLAREKYRMAKKGEK
                     VFRVIEKPVERLN"
     gene            complement(6604..7884)
                     /gene="eno"
                     /locus_tag="PLO58_06120"
     CDS             complement(6604..7884)
                     /gene="eno"
                     /locus_tag="PLO58_06120"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQO74400.1"
                     /translation="MSTIIEVNAREILDSRGNPTIEVEVLTESGFYGSAAVPSGASTG
                     EHEAVELRDGDKSRYLGKGTLKAVQNVNDIIAPEIIGEDVLDQAWIDNLMIELDGTPN
                     KAKLGANATLGVSLAVARAAANFVGLPFYKYIGGVNARVLPVPMMNILNGGKHADNNV
                     DLQEFMIFPVGAKCFAEALRMGVETFHQLKSVLKKKGMNTAVGDEGGFAPNLRSNEEA
                     IEVILEAAAKTGYKIGTELFIALDPASSSFYNTETKKYELKSENRSLSSEEMVDYYKN
                     LVDKYPIISIEDGMAEDDWDGWKLMVKKLGNRIQIVGDDLTVTNVERLSRAIKEKAIN
                     SILIKLNQIGTLTETLNAIELAHRSGFTTVISHRSGETEDTTIADLAVAVNSGQIKSG
                     SACRTDRICKYNQLLRIEEELADDAIFGGTRLFK"
     gene            8144..9112
                     /locus_tag="PLO58_06125"
     CDS             8144..9112
                     /locus_tag="PLO58_06125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQO74401.1"
                     /translation="MSITVEIKQDFPDDQWDRFVYQSVNGTIFHTCQFLSYHPKGRFE
                     DASLIFRQEAELFALFPATRGNWDGYDTLWSHRGASYGGFVYREGLSIKNAYELVEKL
                     IEYARRERIKRIVLTLPPIIYNRRLSNYIDFALIQHGFQYLKREVSSIVSLEENINLN
                     VAKFKPSNRTAFRRAQKLGVVVRESQDYANFYKILKKNLKIRHGVQPTHTLEELVQLK
                     DLFPEKIHLFGAYLADEMVAGVVMFDCNPQVSLAFYISHNEERQECRGVNLLFYEIIK
                     RCIAQGFKFLDFGIFTVNMEPNFGLARFKEGFGSSGILRDTLYLDL"
     gene            9270..10736
                     /locus_tag="PLO58_06130"
     CDS             9270..10736
                     /locus_tag="PLO58_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQO74402.1"
                     /translation="MTASIRSLTKQTLIYGVGTILARLVTFLLLPLYTNILPAAEYGL
                     ASLVFAFNGFMIIIFNYGLDSAMMRYYGESTEPAVRKRLLSTSIWLTISTSLILSGLI
                     YGYSGFLGRVLLGDAARGELLRYSAGILFFDAIAHVPFALLRLEEQPIRFMSIRLLNV
                     IMMFGLNIYFVAHLQRGVAGIFQSNLITSAVTTTILFILIIPRLSLQFSGESARNLVL
                     FGLPFIPAGLATVCMEMLNRYILEHLMGLEAVGVFSAGFKLGIFMLLLTNAFYYAWQP
                     FFLKAGPQESSRLLFARIFTYFVLVELIFWLVLTALMPEIVRFHIGQIYLIGKEYQSC
                     EPMVPIILMGYVFLGIYQMFLPGIYFEKKTRYLAYLTIFAALVNVGFNFILIPSLGIL
                     GSAFSSLIGYFTLAVCSYFVSQRLFRVPYELRRVGLLFLLTLAGGIPIWLFRLNLPIR
                     IAILVVYPLLLWLAGFFKSSEIAKLKQIFSKTTSHHKN"
     gene            10745..12178
                     /locus_tag="PLO58_06135"
     CDS             10745..12178
                     /locus_tag="PLO58_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQO74403.1"
                     /translation="MSGNFRKFFKESTLYLGGLVLNRLVSFLLLPIYTNVFDKTQNGI
                     ITIAYAFLGFMVIILPYGTDAALLNFYTGNDREGKNYFSSAYSLVLISNLLILGLAFL
                     ARNSLSQAVLRVSDPHIFVWCLIILFFDTLNGLTLLVLRAEGRPLTFISFSLANVLLA
                     MGLNIFLVVHRRLGLAGIFYSNILTSGMIWLGLLPLVLKRLDLRLISGKYIRNLVRFG
                     FPFLPAGIFAMILDLSDRWFLEYFTDMETVGLYGVGYKLAAVMLVFVLAFNSAWQPFF
                     LKARREQNTPNIIARVSFFVIGGLAFCWLFFSIWMNYIVRIKISGRYLIGAEFWAGTS
                     VTPLIMLAYFFQALYLLQLPSIFLLEKTKWEPVFRGLGAATNIILNIVFIPRLGMSGA
                     AIATVLAYLAMVIAIWFFNRKYYPIKYDWYKIGAIIVLTAAAYLVAIWGDSVIYRVIA
                     TGIGLLGIGGILLLPLKGRILPILKKK"
     gene            complement(12190..>13371)
                     /locus_tag="PLO58_06140"
     CDS             complement(12190..>13371)
                     /locus_tag="PLO58_06140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO74404.1"
                     /translation="IFIVPSWYPSKLSPENGTFFRDQAQILAKYGDDVTVIVELVHSF
                     KNFLRVKRFSTVKFQPENLGGVMEYRQETLNWFPKRPQKTYFSQRRRLIKLLERAIAE
                     RGRPDLVIAHSSLFAGAELGRWLKDNDIPMIVTEHLMHFLKPEMLTEFYKDCIHEAYC
                     YADRIVATSSRLRDSIVVQFPEFTPKIVVVPNPVDISAFSPASEGKKALKPFVFLCVA
                     LLRPEKRLDLLLRAFATVRQNNPDTQLVLVGDGPQRLNIEHLISNLNLQSSVTLTGYL
                     PQTEIAGWMQKCHTLILPSEVETFGLVLVEAMACGLPVIATRCGGPEDIVTPETGFLV
                     PVNDVTGLTEAMQKMIIEYCQFDSQSIRQTVIDKFSDHKYYWAIHNLYDEIMNNSPKL
                     K"
BASE COUNT         3531 a         2764 c         2989 g         3987 t
ORIGIN      
        1 aaggacaccc cataaaaagg caaaacctgt ggagcaaggt aatggcttcc ggctatcaat
       61 aatatttcat cagattttat ctgaatttct aaaaaattca acagccgttc aagtttatta
      121 tctacataaa tcagcctgga tggaatttgt ttacgaatta cgtcggctaa tatttcggct
      181 gaaactgaag gatggtcagg aatttccgtt aaataaatat ctccaccgag tggctggata
      241 attttaccga tgcgatcgta tttctttacg ctacccagtg ccagtactaa atggaccggt
      301 tgagctggaa atatttgctt tagcgccttt actacctggc gaataccatg ctgattatgg
      361 ccaacgtcga aatagattaa tggcttgtgg gacagtatat gcaagcgtcc gggccaaatc
      421 accccagtga ttccctggcg cagaacttcc gctgaaagtt ccaagtcttt cactcgcatc
      481 agggcactga cagccgcctg taaatttatt agctgatgat caccaattag cggaaactta
      541 accgtgtcaa ttttaagatt atttacaatt agacttaata cctgtcgttc gggaaaacgt
      601 tggatattcg tgatttgaca ttgttcagga gcatagtaaa aatcgccgcc actggcattg
      661 atttctttac ttaaaaagtt gaggacgcgt ggtgtttgac gtgcgactat acaaggaatc
      721 cctcttttga caataccggc tttttcatag gcgatctggg ctagtcggcg accaagaaat
      781 tcctggtgat cacgcccgat gggagtaata acggccactt ccgggagcgt aacattggtg
      841 gcgtcaaaac gtccgcccat accggtttca agaacagcat aatcaacttt atttttttta
      901 aaatagtcca acgccatagc cgttgtggct tcaaaaaaac tgcaatttag ttcgtcaatt
      961 agcttgcgcc aacgttcggt gaatagagca atctcggcat cggggatttt ctggccatta
     1021 attcgaattc gttcgttgaa attcaaaagg tgtggcgaag tatatagtcc gactcgataa
     1081 ccagcggtct gcaaaacatt agccatgatt gcacaagtcg aaccctttcc gttagtcccg
     1141 gcaatgtgta tcaccttata agcgttctgg ggattaccga tcgcatcgag taatttttgg
     1201 atgcgctcta agcctggttt gataccgcga ccattcaaag aatataagta acttaaaata
     1261 ggccgtagat cggcggcatc tgaggatgac attagcaaca atctgcgagg ttgattcgtt
     1321 cgacattggc gattggctcg cccagaaatc gcatcgcgat ttcttcgaac ttctgcggta
     1381 aatcgctcac ataaaaacga tgttccggtg ttctttccaa atcgtttaat agcccctgct
     1441 gattcagaat caagcgtact tgattagccg tttctatggc tgtatctata atctggatat
     1501 tgctatcaat tatccgctga attacgtttt taataattgg gtaatgggta cagcctaaaa
     1561 taagagtgtc aatttttgga acgagattct gcaaatattc ccgtgcaact atttccgtta
     1621 caggtgattc accccatccc tcttcgacaa gcggaacaaa caggggacaa gcctgggaaa
     1681 agacctgcac agatttatcg agagcggtta acacttcaac gtattttccg gaattaatgg
     1741 ttccggtagt accaatgacg ccaatctttt tggtgggcga tattgccagg gctgaccgcg
     1801 ctcctggttc gattacccca ataatcggca agtcgaattg atcttgcagt aattccagcg
     1861 cgacactaga ggctgtatta caagctacta cgatcatttt cacgcttcgt tcggctaaga
     1921 atctggcgat ttggatcgca aatttttgga cggtttcgac gcttttatta ccgtagggca
     1981 ctcgggcagt gtcgccgaag taatatattt gttcctgcgg taattggcga aaaagttgtt
     2041 tgactacggt caacccgccc agacccgaat cgaaaacccc aatgggcaga ttccgcttgt
     2101 tacttacttt gagccactga tttgtcacaa tattccttga attcaataac gcttttataa
     2161 agggctaaag cgattttctg gcgatgagcc ggatcgttca gttttttggc atcgttgcga
     2221 ttgtcattat aaccaagttc acatagtaaa ttgggcattg aagcgccgac caggacgtaa
     2281 aaattggctt gcttgacgcc gcggtttttc tggggcacat attcagaaat attgcgcgat
     2341 aatatttcag cgaatttctc actatcgtgc aagtaggtat tataaaccat attagccagg
     2401 atatgagaag gattatcata gtctttatac ttagccttat cttcgctagt ttctaattta
     2461 atgacttcat tttccaggcg ggcaacttct atagattcct gcgtcctaat agtcggattt
     2521 tggaggtata attcaattcc ctcggcactg cttcttgtac cggatggaag cgcattgatg
     2581 tgcaaactga taaaaagctt accagcagaa tcgttagcta tacgggttct tttccagaga
     2641 ggaataaatt catcgttttt tctggtatag accactttta gatcgttgcg ttgctccagc
     2701 aatttaccca atcgaagagc gatgtccagc acgatatcct tttcgtgttt atagccccaa
     2761 cgaccgggtg tgccagaatc tttccctccg tgtccggcat caatgacaat ggtatcaaca
     2821 atccaggtat ttttgacttc ttcaattttt ttgagcgtag cactggtcag tggcacattt
     2881 aaagtgacta aaattttacc actgcgcgca tcgtaatagg catttgccct gtcatatttt
     2941 tgcttcaatt taagggtaat ttgaaccgaa ttccctactg gaatagcttc aacgattgcc
     3001 actgaaggaa tcggatcccg acgtgaaagc gcggctgaat cacaggtagc cccgagaata
     3061 gtaagataga accactcatc tccgcggaaa aaagaagaaa agtctgaact ttcgtaagta
     3121 gcattggttg aaattttcat cgtcaagccg ttgccgcgtt cttcaaactg gatatcataa
     3181 atgatacccg gctttgtccc aaaagttgtt accgtaggcg ctgatggaat aggagttgcg
     3241 cccggcaatt ctaccagctc ataaatcggt gagcgtttgt cgagataata ttgaagttta
     3301 gggatggcat accgcttcat caagtcaaga aaagagtaca tcggaacaaa aatctcatcg
     3361 cggttgacca aaatagccgg ctgtgacatt tgcagtattc nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn tcataagtca aaggtcaatt gcaaatcacc tgaaaaatca
     3541 aaactgttgt catttgtatg aatagacaca ttgctccgtc catagactgc gccggctatt
     3601 tcacaatttg aaccgattcg ccgactgatt atcagaaaat agcgctgacc agtgccggtt
     3661 aatagagtca tattaaagcg gaaaggcaag ccaggttcat aaaaataaat atttaggtcg
     3721 gaagaaccgg taaaatagtg ggtgttcccg gcagtcacac gggtatattt agaaatcgag
     3781 acttttccca caacgctaag tgcctggcta ccttcagtga aaggaattga atcgttccat
     3841 ctgaaagttg ttctgatttc accggcaaat gacgagctaa aggaatgcct gagatgcaat
     3901 ttaagctgat aatcgcgcgg ttgttgaggc agattggtgt gatagtagcg attgacttta
     3961 taccaagctt caattaagtc gttattgcga gcttgacggc taaatccgca gagggattcc
     4021 cgcccattcc ggattgctac accaggctcg gaatttttta attcacgaaa gaaatcaaca
     4081 tagccagtga accgccagcg agccggtagt ttgattttca gagcggaata taaaccggtt
     4141 tcattgccgg aaagactgga gaacttgcga aaaggattgc cgagcggagc gaaaaaatca
     4201 ggttcgatat agcgccagcc cgcacaaaaa cttacatgat ctctacttag atgaagattt
     4261 tgaattattc cttttttacc ggtttcctgg actacccatt caccgcttat cgtccaatcg
     4321 cttatttcgt aattatgtaa aaatgatagc ccgctaactt tgcgatgtcc gtttaaggca
     4381 aataaaccag tcgaataatt agcattatag accaaaattc cagccgagcc ttggtcccac
     4441 tcgcccagca aacttgctcc tcctaatgtt tccagcaagg cgtctttagc tcggagttga
     4501 cttgagctaa tatgcaagcc atcggagcgc aaatttttga ccgttccatt agtgtcaata
     4561 gtagcatcga gtcggtgatg cgaaagaaaa ggtgttaact taaaatttcc gatagtatat
     4621 tgggcggccg aaccgaaaag atagcggttt tcgtcagacc ccagataatt gcttacccct
     4681 gatcgcgaag tcttcagatt accggttgcg tcagtactgc ggccactcat tagattatta
     4741 gcaaaaagca aaccctggcc ccattgtaag cgataggctc cgcccagaat ttctaaagag
     4801 ttaaaatatt tcggagaacg cagactgatg ttccaataat cacctaaaat aggttcatcg
     4861 ctatcgtttt ccacaattat tcctgtgaac caaccttgag gcgaataaca gcgcgctcgt
     4921 tggagtgatt tctgtaaact agtatattcc tgttttttta aggaaatgaa atttactatc
     4981 tggctcggct tcgttctttt cactgaaaaa tcaataaaat agctgatcgc ttcaatttcg
     5041 gcagttgtaa aaattgtatc ttttaccagt gctttcagat tgaggctctt ggggtagtga
     5101 cgttttagct caattaattg agatatgata tagtcattta gcggcaaagc gcgcaagtcc
     5161 ttaatccggc aagtttccca gaccagcgga ttgtcccgat aagcataaat taattcttgc
     5221 aattcgtcaa tatccaaagc attgtcggta aaataattat cgaggatatc tgccgataag
     5281 tcattttgac caaatgccgg ggcttcggca atagtaatta tcagataact aaataaaagt
     5341 ccaattagaa atttaagccg cattgataat aaacggtata aggcaaagtt ggatgatgta
     5401 aaaacgccac ggctatctgg aatttatacc aggcaagttc caaaccgccc gaaagccgat
     5461 cgggattgaa ttgaactccg gctagcaaat tgcatccgcg aaatacctca aatcgaattc
     5521 cgctccgcca ggtgaatgga aaacgcgtgt ccttgaaaat ttcacccgat atctcaccgc
     5581 gccgaatcgg cttccattgc caaccgaaag cgaaaacctg aggcaaaggt tcttggcgat
     5641 cgcaaatttt gggtgcattg agattttcaa ataaaatagc gaagccgaga gtcgagtcgg
     5701 gaaacaattt taagccgcaa ttcagtccta tagcaccggt tcgtggatag ttggaaattg
     5761 tcaaatcgta ataattgacg gttgctccga gagaaattag cgatgaaatc ttgcgggcga
     5821 gggaaaaacc gattgttttt tcctggtaaa ccagattccc taatgtgttt agtcgcaaag
     5881 ccaagccggt tttggagtgg tggttgtaaa atagaccaat gtcccaggtt tgcaggtttt
     5941 taataccgta tagattggag aagttacagc aggtagataa gccttttttc tcaactaagg
     6001 atgccggatt ggatccatcg gggaacactg atatagttcc accaccggca ataatgatcg
     6061 gagatgcctg atgaatttcg aaagcggctt gagctgtacc aatgcacaac aacaagcaaa
     6121 ataaaaatcg cttttccata ttttcctcct tcaaagcgcc cgcaggttgt gagaacgggc
     6181 tttaatttat caattcaacc ggtgaaagat ataaattctg tgatttaaca ccaattttca
     6241 gagcagtaat cgcgctcggt taatttagac gctcaaccgg tttttcgata acgcgaaaga
     6301 ctttttcgcc tttcttagcc attcgatact tttcacgggc taatttttcg atatactgca
     6361 ggtcgctttc caatcgggtt ttctcgatca ataaagaatc ctgcgtatat tgcaactcta
     6421 ctagatgatt ttcaatctgt tctttttgct tattgagtaa gtagatttgg tatagaccgt
     6481 aatcgccaat tacaaaaaaa atgataaaaa gggtaattgc acaaagtatt ccgagaatcc
     6541 agtaaagttt gtgcaaataa ccggaaccgt ctttactttt aatagatttt ggacggcttt
     6601 ttctcattta aataggcgcg ttcctccaaa aatggcgtcg tcagccagct cttcttctat
     6661 tcgcaggagc tggttgtatt tgcaaatgcg gtccgtgcgg caagctgaac cgcttttaat
     6721 ctggccagaa ttgaccgcca ccgccaggtc ggcgatagtc gtgtcttcgg tttcgccact
     6781 gcgatgggaa atgacagtag tgaatccact gcgatgggca agctcgatag cgttcagagt
     6841 ctcagtcaaa gtaccaatct gattcagctt gatcaatatt gagttgatgg ctttttcctt
     6901 gatggcgcgc gatagacgct cgacattggt aacggttaga tcatcgccga cgatttgaat
     6961 tcgattgccg agtttcttga ccattagctt ccagccgtcc caatcgtctt ccgccatacc
     7021 atcttcaatg gaaataatcg gatatttatc cactaaattt ttataataat caaccatttc
     7081 ttcagaagac aaactgcgat tttccgattt tagttcatat tttttcgttt cagtgttgta
     7141 aaatgaggat gaggctgggt cgagagcgat gaataattcg gtgccgattt tatagccagt
     7201 cttggcggcg gcttctaaaa tcacctcgat agcttcttca ttactgcgca aattgggggc
     7261 aaatccgcct tcatctccga cagctgtatt catacctttt ttcttcaaga cactttttaa
     7321 ttgatgaaag gtttccacgc ccatccgcag agcctcggcg aaacatttgg cgccgactgg
     7381 gaaaatcata aattcctgta gatctacatt attatcggca tgtttaccgc cgttcaggat
     7441 attcatcatc ggaaccggca gaacgcgagc gttgacgccg ccgatatatt tatagaacgg
     7501 caagccgaca aaattagccg ccgcccgagc tacagccagg gaaacgccga gagttgcatt
     7561 agcgccgagt ttggctttgt tgggtgttcc atcgagttca atcataagat tgtcgatcca
     7621 agcttgatcc agaacatctt cgccgatgat ttcgggtgca atgatgtcgt tgacattttg
     7681 cacggctttt aaagtgcctt tgccaaggta gcgcgattta tcgccgtcgc gtaattccac
     7741 ggcttcgtgt tcgccagtgg aagcgccgga cgggacggcg gcactgccgt aaaaaccgct
     7801 ttcagtcaag acttctactt cgatggtcgg attaccacgc gagtccagaa tttcacgggc
     7861 atttacttcg ataattgtac tcattaatta ctccttattt gcttcgattt tttaagataa
     7921 ttcaaatacg ctgtcaattt aatcagctct cggatatttt tcaataaaag tgcgaacgcc
     7981 gaccaaagaa aattaagaaa tcatcaaaaa attgcctata atcaccgaca atcttataaa
     8041 atttcatccg gagattatat gatgaaatgt ccgttttgtg aattggattt gatttagccg
     8101 gtagttgaat ttaaattcaa gccgactttt ttgaggaaat aaaatgtcca ttacggttga
     8161 gattaaacag gattttccag atgatcagtg ggataggttc gtttaccaga gcgtcaatgg
     8221 gactatcttc catacttgcc aatttctatc gtatcatccc aaggggcgct tcgaagatgc
     8281 ttcgctgatt tttcgtcagg aggcggagct gtttgcacta tttccggcga cgcgcgggaa
     8341 ttgggacggc tatgataccc tctggtcgca tcgcggggca agttacggag gatttgttta
     8401 ccgcgaaggg cttagtatta aaaatgctta tgaactcgtg gaaaaattga ttgaatatgc
     8461 gcgacgggaa cgaatcaagc gcatcgtgct aactctacca ccgattattt ataaccggcg
     8521 tttatccaat tatatagatt ttgcgctgat tcagcacggc tttcagtatt tgaagcgtga
     8581 ggtttcgagt atcgtttcat tggaagaaaa tatcaacctg aatgtcgcga agtttaaacc
     8641 atctaaccga acggctttcc ggcgggcgca aaaattaggc gtggtcgtgc gggaatcgca
     8701 ggattatgcc aatttttata aaattttaaa gaaaaacctc aaaatccgcc acggcgttca
     8761 accaactcat acattggaag aactggtgca attaaaggat ctatttcccg aaaagattca
     8821 tttgttcggg gcttatctgg ccgatgaaat ggtcgccgga gtggtaatgt tcgattgtaa
     8881 tccgcaagta tcgctggctt tttacattag ccacaacgaa gaacggcagg aatgtcgcgg
     8941 cgtcaatctc cttttttatg aaattataaa aagatgtatt gcgcagggtt ttaagtttct
     9001 cgactttggt atttttactg taaatatgga gccgaatttc ggactagctc gatttaagga
     9061 gggatttggt tcgagcggca ttctgcggga cacattatat ctcgatcttt agaataaatt
     9121 ttagcggttt tataataaag caaggaaacg acggggaatt agtaagtact tggtgaatag
     9181 gaatagacag gcaagaatgc ctgtcctaca gttggcgctg attctgcgga aaagttcgta
     9241 tatttgggcg ttttcgatct ttaattaata tgaccgcaag tattcgtagt ctcaccaagc
     9301 aaactctgat ttatggcgtc ggcaccattt tagcccgctt ggtgactttt ttactattgc
     9361 ctttatatac gaatattctg ccagcggctg aatatggttt ggcgtctctg gtttttgctt
     9421 tcaacgggtt tatgataata attttcaatt acggtctgga ttcggcgatg atgcgctatt
     9481 atggcgaaag caccgaaccg gctgtaagga agcgcttgtt atcaacttcg atctggttaa
     9541 cgatctcgac cagcctgatt ctgagtggtt tgatttacgg ttacagtgga tttctcgggc
     9601 gcgttttatt aggcgacgcg gcgcgtggtg aactcctgcg ttattcggcg ggaattcttt
     9661 ttttcgatgc cattgcgcac gtgccgtttg cgcttctacg tcttgaagaa cagccgattc
     9721 gatttatgag tattcggctt ctgaatgtta ttatgatgtt cgggctgaat atttattttg
     9781 tcgctcatct tcagcgcgga gtagctggaa tctttcagag taatctgata acatctgccg
     9841 ttacaactac tatacttttc attttaatta ttccaaggtt aagtctgcag ttttcgggag
     9901 aaagcgcccg caatttggta cttttcggtc tgcctttcat tcccgccggg ctagctacgg
     9961 tttgtatgga aatgcttaac cgttatattc tggaacactt gatgggtttg gaggcggtgg
    10021 gcgtttttag cgccggtttc aaattgggaa tatttatgct tcttttaacg aacgcatttt
    10081 actacgcctg gcaacccttt ttcctgaaag ctggcccgca ggaatcgtcg cgcctgttat
    10141 ttgctcggat tttcacctat ttcgtgttgg ttgagctgat tttctggttg gtgctgactg
    10201 ctctaatgcc ggaaattgtg cgtttccaca ttggtcagat ttatttaatc ggtaaggaat
    10261 atcaatcttg cgagccgatg gtgccgatta ttttgatggg atatgttttt cttggaatat
    10321 accagatgtt tctcccggga atttattttg aaaagaagac ccgttatctg gcttacctga
    10381 ctattttcgc ggctttggta aatgtgggct ttaattttat acttattccg agcctcggaa
    10441 ttctcggttc ggctttttca tccttgattg gatattttac tttagctgta tgcagttatt
    10501 ttgtttcgca acgccttttc cgggtgcctt atgaattgcg gcgagttggg cttttgtttc
    10561 tattaactct ggctggtggg attcctattt ggctgttcag actaaatctg ccgattagaa
    10621 tcgctattct ggtagtttat ccgcttcttt tatggctggc tggttttttc aagagttccg
    10681 agatagcgaa attgaaacag atattttcca aaactacctc gcatcataaa aactgaatat
    10741 gaatatgtcg ggtaatttca gaaaattttt caaagaatcg acgctatact taggcggctt
    10801 agtgctaaat cgcctcgtaa gttttctatt acttccaatt tacaccaatg ttttcgataa
    10861 aactcagaac ggcattataa cgattgctta tgcttttctg ggatttatgg tgattatttt
    10921 accatatggc acggacgccg ccctgctgaa tttctatacc ggaaatgacc gcgaaggtaa
    10981 gaattatttt tcgagtgcct attcgctagt tctgatttcg aatttgctaa ttctgggatt
    11041 agctttttta gcgcgcaaca gcctatcgca agccgtacta cgcgtgagcg atccccatat
    11101 tttcgtttgg tgcttgatta ttttattctt cgataccttg aatggcttga ccttgctcgt
    11161 cttacgagcg gaaggccgcc cgctgacttt catctcgttc agtttggcaa atgtcttgtt
    11221 ggcaatggga ttgaatattt ttctggtagt gcatcgcagg cttggcttgg caggaatttt
    11281 ttacagtaat attctaacat cgggaatgat ctggctggga cttttaccac tggtactaaa
    11341 acggctggat ttacgcttga tttcgggcaa atacatcagg aatttagtca ggttcgggtt
    11401 cccctttcta ccggcaggaa ttttcgcaat gattttggat ttatccgatc gctggtttct
    11461 cgaatatttt accgatatgg aaacggttgg attatatggc gttggctata aattagccgc
    11521 ggttatgctg gttttcgtgc tggcgtttaa ttccgcctgg cagccctttt tcctgaaagc
    11581 gcggcgtgag caaaatactc ctaatattat tgccagggtt tccttttttg ttatcggggg
    11641 attggccttc tgttggttgt tttttagtat ctggatgaat tatatcgtga ggattaaaat
    11701 ttccggccga tatttgatcg gtgcggaatt ttgggctggt acgagtgtca caccgctcat
    11761 aatgctggct tacttttttc aagcacttta tttgttgcaa ttaccgagta tttttcttct
    11821 ggaaaaaact aaatgggagc cggtttttcg cggattaggc gcggctacta atataattct
    11881 aaatattgtg ttcataccgc gcctgggaat gagcggggcg gcgattgcga ctgttctggc
    11941 gtatcttgcg atggtaattg ccatctggtt ttttaaccgg aaatattatc cgataaaata
    12001 cgattggtat aaaattggag cgatcattgt tttgaccgcg gcggcttatc tggttgctat
    12061 ctggggagat tcggttattt accgggtaat agctactgga atcgggttat tgggaattgg
    12121 tggaattctt ctcttgccat tgaagggtag aatattgccg attctgaaaa aaaagtaaca
    12181 aacgaaaatt cactttagtt taggagagtt attcataatc tcatcataga gattgtgaat
    12241 tgcccagtaa tatttatgat cgctgaattt atctataact gtttgtcgaa tggattgaga
    12301 atcgaattga caatattcga taatcatttt ttgcattgct tcggtcagac cagtaacgtc
    12361 attaactggc accaaaaacc cggtttcggg ggtgacaata tcttccgggc caccacagcg
    12421 ggttgcaatg actggcagac cgcaagccat agcttcgacc agaaccaggc caaaagtctc
    12481 gacctcgctg ggtaaaatta gcgtgtggca tttttgcatc cagccagcga tttcggtctg
    12541 cggcaaataa ccggtcagag taacggaact ttgcaggttc aaattagaaa ttaggtgctc
    12601 tatatttaga cgttgcggcc catctcctac gagaacaagc tgggtatcag gattattttg
    12661 ccgaacggtg gcgaaagctc tcagcagaag gtcgagcctt ttttcggggc gcagaagagc
    12721 cacacaaaga aagacaaatg gttttagtgc cttttttccc tcggaagcgg gagaaaaagc
    12781 cgatatatcc accggattcg ggacaacgac gattttgggg gtaaattctg ggaattgaac
    12841 tacaatcgaa tcccgcaatc gggaactggt agccacgatt cggtcggcgt agcaatacgc
    12901 ttcgtggata cagtctttat agaattcagt tagcatttcc ggtttcaaaa aatgcataag
    12961 atgttcggtg acaatcatcg ggatgtcatt gtctttcagc caccgaccga gttctgctcc
    13021 ggcaaataat gacgagtgag cgatgactaa atccggtcgc ccgcgctcgg caatcgccct
    13081 ttcaagtagt ttgatcaagc gccgccgttg ggaaaaataa gttttttgag gtcgtttcgg
    13141 aaaccagttt aaggtttcct gacgatattc cataacaccg ccgagatttt ccggttggaa
    13201 cttgacagta ctaaatcttt taacccgcag aaagttttta aaagaatgca ccagttctac
    13261 gattaccgtg acatcatcgc cgtatttcgc aagtatctga gcctggtcgc ggaagaatgt
    13321 gccgttttca ggcgagagtt ttgatggata ccacgacgga acaatgaaga t
//
LOCUS       DAOMJU010000051        13179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_4727_length_13179_cov_19.312481, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000051 DAOMJU010000000
VERSION     DAOMJU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13179
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4727_length_13179_cov_19.312481"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PLO58_06145"
     CDS             complement(<1..1205)
                     /locus_tag="PLO58_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74405.1"
                     /translation="MKKILSIFMIILTVSTLVIGAEDIYKTRALEKAAMTSDIWVNAN
                     RMNGVFRNNGTWFYDNVLGDWGLEWPKGSGLSPIFGAGQYIGAKIDGEIRVAGVQHSA
                     TEFQPGLILATDVADNKADPKYKWYEIRPNGVGDWTNWPTDQGAPLDKDGKPMLIGDQ
                     TIYSVWNDLAPHSEYGTNKLNAEVHQMAWAFSRADAMGDMIFIKWTLVNKSGKNWEDT
                     YFVIWSDPDLGNAGDDQVGCDSTLGLGYCYNATDNDQVYGAAPPAVGIDFFQGPIVPE
                     VGSVVALPDGTVLQDKKMLKMTSFIYYNNDDSNQGNPSSGMDVYNYMSGFWRDGTPIT
                     EGGVGTDPSKPPTKFMFSGDPETGIGWLDSNPADRRFFMTTGPFTMEPWVDSDGDGMA
                     EFGEPGVQE"
     gene            complement(1249..4227)
                     /locus_tag="PLO58_06150"
     CDS             complement(1249..4227)
                     /locus_tag="PLO58_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO74406.1"
                     /translation="MARRVAICLLAMLLPIVLFAGTSGKIAGSVVDKNTGAPLAGVNI
                     IVTGGGYNLGAATDLDGYYSILNVPVGTYTVRSSYIGYKEIVYNNVAVSLDLTTELNF
                     EMERTTLEFEQVVVTAERPMIRKDATNTNIIKSAEEIANMPLRGMQEIAASTAGVVKA
                     ENSTAMNIRGGRSAETALYIDGVLVNDPYNNAVYTYLPNEAIAEMSVQTGGFNAEYGD
                     AMSGIVAITTNAGSENYSASFQVITDEFLKADEKILGTYGYGFNEYVASIGGPIIPKS
                     NHTFFISGTRRYAADAWPSYGWAENDYRLDDFTYDQVLTKIDDQGNTFLDTITHNYDL
                     TARIPGNSTSDWSWNAKLKFQLGKNMVLKTSYVSTDRIISSVTPIDIFNTEHMGEESR
                     YSHSFNATLTHTIRQNTYYDLKFNWFDTKRKIYDATFGDDLVKYGDPWLNPWPDDNAY
                     VGVAYTGRITPDYFQPGCQYDDYFKNRTTYWGIDFDIVHQMGKNHTFKAGFEYKYHTL
                     REYRVLAPVYLAKTNLSDLEKYQSADVRFYGYDIDGKEIEEGDFLEDAVYDDAGRLAD
                     GYQKQAPYHPIILSGYIQDKIEFRDLVLNIGLRYDHINPNAWQFKQLDAEWAADGSYI
                     PGTGMFGGNEKFDKTDVEDSEVHTYISPRLGISFPVTDQTVFHAQYGQFYQAPSLQDL
                     YLSPFYLDAFVSRGGYFTTLDNPNLKPPKTTSYEIGFKQMLGNLASVQLTTFYKETED
                     LIQVMNVTTDVTNIAFSRNGDFGTVKGLDIIFNLRRYKNLAATFNYELQYATGTGSAT
                     NSNFNIAWQGGARGNFPKFAMPLDFEQRHTGSLNLDYRLGRTEGPKFLHNAGANLMFS
                     FNSGHPYTLMQVFNTMPFTGRYDNTNVSETPVSAVNSQTTPWVFRVDLKLDKKFYIGG
                     VQLTAYAWALNLLNTENVANVWITTGLPDDTGYLGTSAGRQYWNSLTDEQKSLYKMRE
                     MDYNHYGSPRQIRLGLMLEL"
     gene            complement(4352..4957)
                     /gene="clpP"
                     /locus_tag="PLO58_06155"
     CDS             complement(4352..4957)
                     /gene="clpP"
                     /locus_tag="PLO58_06155"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HQO74407.1"
                     /translation="MKTKAQAYVPFVIEQTERGERSYDIYSRLLKERIIFLGLPIDDH
                     VANLVIAQLLFLAAEDAKKDIYLYINSPGGSITAGMAILDTMNYIKPDMVTICLGLAA
                     SMAAVLLASGTRGKRQALPNSRIMIHQPLGGVEGQASDIEIHAREIIRLKENTNRILA
                     ELTGQPLEKINRDTDRNYFMSSEEALKYGIIDEILPARIKK"
     gene            complement(4970..6238)
                     /gene="tig"
                     /locus_tag="PLO58_06160"
     CDS             complement(4970..6238)
                     /gene="tig"
                     /locus_tag="PLO58_06160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQO74408.1"
                     /translation="MKAELINSTPIEKTVKVVVDWDEIADDYIAVFNDLRRDLKIDGF
                     RKGKVPESLAKRLLKPQIFVSFTNQIIEKTHKQALKDVGMDDYLDLHIAELDFNENTP
                     FNYTIKVEYDPEITLPDYKKGIKIQQKEYIVDPETVERYIEDFLENQAEVQEVFDGAQ
                     DGDFLVADLQALDEGGIPLIGKRLPDRLIKVGEGIFGDKEAGNFKGVKAGDSVNLNVK
                     SSSGKMLKYLVDVKRVEKHTLPELTDELVKSQVEDVQTVAEFRQRITENLQYKWNQRA
                     QEELEQALADYFIENTKFDIPPYRINSYLDSLIEKMKENSQNKPIDEAKIREEYLPIA
                     EKNIRWYLIQQAVRKQEDIKVSEDEIQTEIDKILQIVPEEQKELAEQFYQKPRNRLEI
                     RVNLEDRKIREHIHQFIKEKKVTVHTESLQ"
     gene            complement(6276..6358)
                     /locus_tag="PLO58_06165"
     tRNA            complement(6276..6358)
                     /locus_tag="PLO58_06165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6322..6324),aa:Leu,seq:tag)
     gene            complement(6513..9584)
                     /gene="secA"
                     /locus_tag="PLO58_06170"
     CDS             complement(6513..9584)
                     /gene="secA"
                     /locus_tag="PLO58_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQO74409.1"
                     /translation="MGIATYLTKIFGTSSDREIKKILPLVAEINRIYATLSEQPIEKL
                     RERTAELKVQIQTAREAARIEAEESGISEEEAQKLIQTAEQNALNAILPEAFAMVKEV
                     CRRLVGQEWLVTDQKIVWDMVPYDVQLIGAIVLHQGKIAEMKTGEGKTLVATMPVFLN
                     ALTGRGVHIVTVNDYLAQRDSEWMGRIYTELGLTVGCILNSMDSEERKKMYACDITYG
                     TNNEFGFDYLRDNMALSAEEQVQRSYYYAIVDEVDNILIDEARTPLIISGPVQSSRHE
                     KFDELNPAVGTIVRYQRDKITEILSRFPDSPESIPEKDYSEFARQLFIASQGMPKNRR
                     LRKLLEEPGYQKMLQSGEKEYLLLTNAKTGTSISQDEFFKDLYYFIDEKNNNITLTPT
                     GEEKLATLLGIKLDELIIPDLAEEFVKIDENPELDEVEKEKRKMELDTQHSEISDRYH
                     NISQLLRAYTLFEKDVEYVIQDGKVLIVDEFTGRILPGRRYSDGLHQAIEAKEKVKVE
                     AETQTFATITLQNYFRMYEKLAGMTGTAATESSEFADIYKLEVVVIPTHKPCIRIDYE
                     DQVYKTRREKYNAIINEIETSFRRGQPVLVGTISVEVSETISRMLKRRGIPHNVLNAK
                     NHKREAEIVARAGERGAVTIATNMAGRGTDIKLGQGVVELGGLYIIGTERHESRRIDL
                     QLRGRSGRQGDPGASRFFLSLEDDLMRLFNSERIAAIMDRLGVEEGQVITHPMVTRSI
                     ERAQRRVEERNFTIRKNLLEYDDVMNHQREIIYARRNYALKGSDTKGELYNILEDWAD
                     DLVQKYNAQVKDSGDWQWTALRMDLTASLGIDLDPSELSEYTPEALKQAIIDRAKQYY
                     ERKEKIYGAEQMRFLERFIMLRTIDEKWRDHLYAMDQLKEGINWRAYGQKDPLLEYKG
                     EAFDAFVQMVNDINQQTLRLCFRAQIGGPQPETRQVRRPSTMRLMHQQADGMGFIPGM
                     QSETDEQPQYATSDGQQAKIRPVRVEAKVGRNDPCPCGSGKKYKYCHGAGN"
     gene            complement(9666..10127)
                     /gene="nusB"
                     /locus_tag="PLO58_06175"
     CDS             complement(9666..10127)
                     /gene="nusB"
                     /locus_tag="PLO58_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQO74410.1"
                     /translation="MRERRAAREVALQALYAEELTSYPIAQIIDDVIENSELQDELKT
                     FAKDLFIAAVTHRDELDEIIREKSENWDFERIAIIDRLVIRMAISEFKYFNDIPPKVS
                     ISEAIEIAKIYSTDDSSAFVNGILDAVLHDISDTLTYKLKRSETKSATREE"
     gene            10507..12873
                     /locus_tag="PLO58_06180"
     CDS             10507..12873
                     /locus_tag="PLO58_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQO74411.1"
                     /translation="MPPKKIDNTQIPTSNVEKYYLSLSGRQFIYPPTEKIPVIQVANF
                     PMLGQLTALRFLEWVQNNPDGVIALPTGKTPEHFIKWVGYYLTNWHSAEVIKDLEMVE
                     IDPARRPDMARLHFVQIDEFYPIDPNQQNSFYYYIQKYYIKNFGLDPEKALLINLYEI
                     GTANNLSIAEIFGDEKADLSLRTRWAKNPIEELQKETIARTDQFCVNYESKIRALGGI
                     GFFLGGIGPDGHIAFNIRGSDHYSTTRLMETNYETQAAAASDLGGIEIARKRLVITIG
                     LDTITYNKDATAIIIAAGQAKANIVRDSIQNFPSIQYPATVLQKLPNARFYLTEGAAS
                     KLFERRYEKMAKSPELEPKTIEKAVINLTLEKNKKLTDLTLADFNSNRFTELILKRTN
                     QTHTEICRNLHESIINRIDRGIYYLDNEVFMHTSPHHDDEMLGYLPYIVHLVRNPRNK
                     HYFTYMTSGFTAVTNSYVLERLKNLQDLVDSPTFQVLMQEHYFDPANTAARNRDVNLY
                     LDGVALYDVKKKTEAESRRLLSILTEIYEENNVIYLKDRISELINYCATQYPGKKDIA
                     HIQKLKGMIREWEADVLWGYFGFNSNNVFHLRLGFYKGDIFSEEPTLDRDVLPIYQLM
                     KKIRPTVITVALDPEGSGPDTHYKVLQAITEALKLYEKEEDISNLRIWGYRNVWHRFH
                     PASTNIYVPVSLNSMAIMENAFMESFGSQRSASFPSWEFDGPFCRLARKIQVDQYLKI
                     KTCLGRDFFIKNEHPRLRATHGFVFIKELTPKEFYRHSEESRRVTENL"
BASE COUNT         3715 a         3083 c         2490 g         3891 t
ORIGIN      
        1 atttcctgca caccgggttc accgaattcg gccatgccat caccatcgga gtcaacccac
       61 ggctccatcg tgaacggtcc ggtcgtcata aagaaacgcc tatcagcagg attagaatcc
      121 aaccaaccaa ttccagtctc cggatcaccg gaaaacatga actttgttgg cggtttactt
      181 ggatctgtac caaccccacc ttcggtaatc ggcgtaccgt ctctccagaa accactcata
      241 taattataaa catccattcc agaactggga ttaccctgat tggaatcatc attattataa
      301 tagatgaaag acgtcatctt aagcattttc ttatcttgta agacagtccc atccggtaag
      361 gcaactacac ttccgacttc tggtactatc gggccttgaa agaaatcaat accgacagct
      421 ggtggtgcgg caccataaac ttgatcgtta tcggtcgcat tgtagcagta accaaggcct
      481 aaagtcgaat cacaaccaac ctgatcatca ccagcatttc ctaaatcggg atcagaccag
      541 ataacaaagt aggtatcttc ccagttttta ccactcttat tgactaaagt ccatttgata
      601 aagatcatat cacccatcgc atcagctcgg ctgaaagccc aagccatttg atgaacttcg
      661 gcattcagtt tatttgtgcc atattcagaa tggggtgcca ggtcattcca gacgctatat
      721 atcgtctggt cgccaatcaa catcggcttt ccatctttat ctaatggtgc cccctgatcg
      781 gtaggccagt tagtccaatc accgacgcca ttaggcctga tttcatacca cttatatttg
      841 ggatcggctt tattgtcagc aacatcggtt gctaaaatca aaccgggttg aaattctgtt
      901 gcagagtgtt gcacaccagc gacacgaatt tcaccatcaa ttttagcacc gatatattgt
      961 ccagcaccaa agattggact cagcccacta cctttcggcc attccaatcc ccaatctcct
     1021 aatacgttat cataaaacca agtcccatta tttcgaaaaa cgccattcat tcgattggca
     1081 ttcacccaga tatcactggt catggctgct ttttccaacg cccgggtttt ataaatatcc
     1141 tctgctccaa ttacgagcgt cgaaaccgtt aatattatca taaatattga caatattttt
     1201 ttcatatact ttcctccatt ataatgagtt atcttcatag ttcgctcgtt atagttcaag
     1261 catgagtccc aggcgaatct gacgcggcga gccataatgg ttataatcca tctcgcgcat
     1321 cttatataga ctcttctgct catcagttaa actattccaa tattgccggc cagccgatgt
     1381 ccctaaatat ccagtatcat ccggtaaacc ggtagtaatc cagacatttg cgacgttctc
     1441 ggtattcagt agattcaaag cccaggcata agctgttaat tgaacaccac caatgtaaaa
     1501 ctttttatcc agtttcaaat caactctaaa aacccagggt gtcgtctgag aattaactgc
     1561 tgataccggc gtctcggaaa cgttggtatt gtcataacga ccggtaaaag gcatagtatt
     1621 gaaaacctgc atcagcgtat agggatgccc cgaattgaaa ctaaacatca agtttgcccc
     1681 ggcattatgt aaaaacttag gaccttcggt tctaccgaga cgataatcta aatttaaact
     1741 accagtatgt ctttgctcga aatccagcgg cattgcaaat ttcgggaaat ttccacgagc
     1801 gccaccctgc caagcgatat taaagttgga atttgtcgct gaaccagtac cggtcgcata
     1861 ctgtagctca taattgaatg tcgccgctaa attcttatag cgtctgagat taaaaataat
     1921 atccaagccc ttgacagttc cgaaatctcc attccgactg aaggcaatat tggtcacatc
     1981 cgtagtaacg ttcattacct ggattaagtc ttcagtttct ttatagaaag tggtcaactg
     2041 cacacttgcg aggttaccca gcatctgttt aaaaccaatt tcataggaag tagtcttcgg
     2101 cggtttcaga ttcggattgt ccagagtcgt aaaatatccg cctctgctta cgaatgcatc
     2161 cagataaaac gggctcaagt ataaatcttg caggctcgga gcttggtaaa attgcccata
     2221 ctgcgcgtga aaaacagttt gatcggttac aggaaaactg attcccagac ggggactgat
     2281 ataggtatgt acttcagaat cttcgacatc tgtcttatca aatttttcat ttccaccaaa
     2341 cattccagta cccggtatat aactgccgtc agccgcccat tcggcatcca gttgtttgaa
     2401 ctgccaggca ttcggattaa tatgatcata tctgagaccg atatttagca ctaaatctct
     2461 gaattcaatc ttatcctgaa tgtagccgct cagaataatc gggtgataag gagcctgttt
     2521 ctggtaacca tcggctaatc ttccagcgtc atcataaacg gcatcttcaa gaaaatcacc
     2581 ttcttcaatc tctttaccat caatatcata gccataaaaa cggacatcgg cagactgata
     2641 tttttcaaga tcagataggt ttgtctttgc tagataaacc ggtgccagta cccgatattc
     2701 tctcaacgtg tgatatttat attcgaagcc ggctttaaaa gtatgatttt tacccatctg
     2761 gtgaacaatg tcaaaatcaa tgccccaata agttgtccgg ttcttgaaat aatcgtcata
     2821 ctgacaacct ggctgaaaat aatccggtgt aatgcgaccg gtataagcaa ccccgacata
     2881 ggcattgtca tccggccagg gattcaacca cggatcacca tattttacta gatcatcacc
     2941 gaaggttgcg tcataaatct tccgcttagt atcaaaccaa ttgaatttca ggtcgtaata
     3001 ggtgttctgt ctgatcgtat gggttagggt agcattgaat gaatgcgaat aacgactttc
     3061 ttctcccata tgttcagtat tgaaaatatc aatcggagtg acggaactaa tgatgcgatc
     3121 ggtagaaaca tagctggttt tcaataccat atttttaccc agctggaact tgagcttggc
     3181 gttccatgac caatcagacg ttgaattgcc tggtatgcgt gctgttagat cataattatg
     3241 ggtaatagta tctaagaaag tattaccttg atcatcaatc ttagttaaaa cttgatcata
     3301 cgtaaaatca tccaaacgat aatcattttc cgcccaccca taggatggcc aggcatcagc
     3361 cgcataccgc cgtgtcccgc tgataaaaaa tgtgtgatta cttttcggta taattggtcc
     3421 accaatagaa gctacatatt cgttaaatcc ataaccgtaa gtacccaaga ttttctcatc
     3481 agcctttaaa aattcatccg taattacctg gaatgatgca gaataattct cactgccggc
     3541 gtttgttgta attgcaacga taccggacat agcatcacca tattctgcat taaaaccgcc
     3601 ggtctgaaca ctcatctccg cgatggcttc gttcggcaaa taagtgtaaa cagcattatt
     3661 gtatggatca tttactaaaa caccatcaat ataaagagct gtttcagcac tacgtccacc
     3721 ccggatattc atagcggtgc tgttttccgc tttgacaaca ccagcagtac tagcagcaat
     3781 ttcttgcata cctcgtaaag gcatattggc aatttcttct gctgacttaa taatgttggt
     3841 gttagtagca tcttttctga tcattggcct ttcggcggtc accaccactt gttcaaattc
     3901 caacgtagtt cgctccatct caaaatttaa ctctgtggtt aaatcgagtg acacagcgac
     3961 attgttgtaa actatctctt tatagccaat gtagcttgat cgcacggtat aagtaccaac
     4021 aggaacattg agaatgctgt aatagccatc cagatcggtg gctgccccaa gattatatcc
     4081 accaccggta acgataatat ttacaccggc tagaggagca cctgtgttct tgtcaacaac
     4141 tgaacctgcg attttaccag aggttcccgc gaagagaaca ataggtagca acattgctaa
     4201 aagacaaatc gctacacgac gtgccataca aatcctcctt ttttaatact attgttgttt
     4261 gttcaattac attacctcac tgcatggtaa atgtaaagaa tttatttttc taatgtcaag
     4321 cttttttatt ctatttttgt ataaaaataa attatttttt tatccgcgcg ggtaaaattt
     4381 catcaattat cccatatttc agggcttctt cgctcgacat aaaatagttg cggtcagtgt
     4441 ctcggtttat tttttcaagt ggttgaccgg tcaattccgc caagattcga ttcgtatttt
     4501 cctttaaacg aattatttca cgagcgtgaa tctcgatatc gctggcttgg ccttccacgc
     4561 cgcccaatgg ttgatgaatc ataatgcgcg aattgggcaa agcctggcgt ttgcctctgg
     4621 taccgctggc gagtagaacc gccgccatgc tggcggccaa accgagacaa atcgttacca
     4681 tatccggctt aatgtaattc atcgtatcca gaatcgccat tccagccgtt atggagccac
     4741 ccggactatt tatatataaa taaatgtctt tcttggcatc ctcagccgcc agaaaaagca
     4801 attgggcaat taccaaatta gccacgtgat cgtctatcgg cagtccaaga aatataatgc
     4861 gttccttgag caatcgcgag taaatatcat acgaacgttc cccccgctcg gtctgctcaa
     4921 tgacaaatgg aacataagct tgagccttgg ttttcatcgt gacctccttt tattgtaagg
     4981 attcggtatg aacggttact tttttctctt tgataaattg atggatatgc tcccgaatct
     5041 tgcgatcttc caggtttacc ctaatttcca gccggttgcg aggtttctgg tagaattgtt
     5101 ccgctaactc tttctgctct tccggcacaa tttgcaggat tttatctatc tccgtttgga
     5161 tttcgtcttc gctaacctta atatcttctt gttttcgaac agcctgttga ataagatacc
     5221 accgaatatt tttttcggca attggtaaat attcttccct gattttggct tcgtcaatgg
     5281 gtttattttg ggaattttct ttcatttttt caattaaact atccaaatag gaattgattc
     5341 ggtaaggcgg aatatcaaat tttgtatttt caataaaata atcggcaagg gcttgctcta
     5401 attcttcctg agcccgctga ttccatttgt attgcaggtt ttccgtaatt cgctgacgaa
     5461 actccgccac ggtttgaaca tcctccacct gacttttaac taattcatca gtaagttcgg
     5521 gtaaggtatg tttttcaact cgttttacat ctaccaagta ttttagcatt ttaccagacg
     5581 acgatttcac gttcaagttg acactatcac cggctttgac ccccttaaaa ttaccggctt
     5641 ccttatcgcc gaaaatccct tcgccgactt taatcagccg atccggtaag cgtttgccaa
     5701 ttagcggaat gccgccttca tccagagcct gcaaatctgc cacgagaaaa tcaccatctt
     5761 gagcaccgtc aaaaacttcc tgcacttccg cctgattttc cagaaaatct tcgatgtaac
     5821 gctcaacagt ttccggatca acaatatatt ctttctgctg aatttttatg ccctttttat
     5881 aatcaggcaa agtaatttcc ggatcatatt cgactttaat agtgtaatta aaaggtgtat
     5941 tttcattgaa atccagttca gcaatgtgca aatcgagata gtcatccatt cccacatctt
     6001 tcaacgcttg cttatgagtt ttttcgataa tctgattagt aaaactcacg aaaatttgag
     6061 gcttaagcag gcgtttagcc aaactttccg gcactttgcc cttgcgaaac ccatcaattt
     6121 tcaaatcgcg gcgtaaatcg ttaaatactg cgatataatc gtcggcaatt tcgtcccagt
     6181 caaccaccac tttaactgtt ttctcgatcg gtgtactatt aattaattcc gctttcattg
     6241 aaattcctat ctatttattt tttcaatttg agaagtgccg cgggcgagat ttgaactcgc
     6301 acccctatta aggactggat cctaagtcca gcgcgtctgc caattccgcc accgcggctc
     6361 aaaaaacgcc caaatttaac cgccactctg cgattttcca aagtgattaa caatgataat
     6421 cgtgtaaaaa tagtgacttt agataatttg acctaatcaa cttacgcttc tcatatatga
     6481 tagattacaa attaccagaa aaaatccacc ggtcagttac cggcgccatg acagtatttg
     6541 tattttttcc cactaccgca ggggcaggga tcattgcggc caactttggc ttcaacccgc
     6601 accggtcgaa ttttagcctg ctgtccatca ctggtggcgt attgcggttg ttcatcagtc
     6661 tccgattgca ttcccgggat gaaacccata ccgtcggctt gttggtgcat caagcgcatc
     6721 gtagaagggc ggcgaacctg gcgagtttca ggttgcgggc cgccaatttg ggctcggaaa
     6781 cagagtcgca aggtttgttg attaatgtca tttaccattt gaacaaaagc gtcgaatgcc
     6841 tcacctttat attccagcag aggatccttt tggccataag cgcgccagtt tatcccctct
     6901 ttgagttgat ccatagcata taagtgatca cgccattttt cgtcaatagt acgcaacatt
     6961 ataaaacgtt ccagaaagcg catctgctca gcgccgtaaa ttttttcctt ccgttcgtaa
     7021 tattgcttag cgcggtcaat aatggcttgt ttaagcgctt caggtgtata ttcggataat
     7081 tcactggggt ctaaatcaat tccaagactg gcagtaaggt ccatccgcaa tgccgtccat
     7141 tgccaatcac cagaatcttt aacctgagcg ttatattttt ggaccaggtc gtctgcccag
     7201 tcttcaagta tgttatacag ttcgccttta gtatcactac ctttcagggc ataattgcga
     7261 cgggcgtaga taatttcacg ctgatgattc attacatcgt catattccag caagttcttg
     7321 cggatagtga aattgcgctc ctcgactcgc ctttgtgctc gttcgatcga acgggttacc
     7381 atcggatgag taatgacctg accttcttcg acaccgagcc gatccataat tgccgcaatg
     7441 cgctcactat taaaaagtcg cattaaatca tcttcgagcg aaaggaaaaa gcgtgatgct
     7501 cccgggtcgc cttgacgtcc gctacgaccg cgcaattgca aatcaatgcg gcgggattca
     7561 tggcgttccg taccgatgat atagagccct ccaagttcaa caacgccttg ccctaactta
     7621 atatcggtgc cacgtccagc catattggta gcaattgtca cggcaccccg ttctccggct
     7681 cgcgccacaa tttctgcctc acgtttatga ttcttagcat tcaatacgtt gtgaggaatg
     7741 ccacgtcgtt tcaacatacg gctaatcgtt tcggaaacct caacgctgat agtaccgacc
     7801 aacaccggct ggccacggcg gaaggaagtt tcaatttcat taataatcgc attgtatttt
     7861 tcacgacgag ttttatagac ttgatcttcg taatctatgc ggatgcaggg cttatgggtc
     7921 ggaattacta caacttctaa tttgtaaata tccgcaaatt cagatgattc ggtggcggct
     7981 gtaccggtca ttcccgctaa tttttcatac attcggaagt aattttgcag ggtaatcgtc
     8041 gcaaaagttt gggtttcggc ttcgaccttg accttttcct tggcttcgat agcctgatgc
     8101 aatccgtcac tatagcgccg ccccggcaga atccggccgg tgaattcgtc cacgatcagg
     8161 actttaccgt cctggataac atactcgaca tccttttcaa ataaggtata agcccttagt
     8221 aattgcgaaa tattgtgata ccgatcggag atttccgaat gctgggtatc gagctccatt
     8281 ttgcgctttt ccttctctac ctcatctagt tccggattct cgtcaatttt gacgaattcc
     8341 tcagccagat caggaattat gagttcatcg agcttgattc ctaacaatgt agccagtttt
     8401 tcttcaccgg taggagttaa agttatgtta ttattttttt cgtcaatgaa gtaatataaa
     8461 tccttgaaaa attcatcctg actgattgaa gtcccggttt tggcattcgt gagtaacaaa
     8521 tattccttct caccactttg taacattttt tgataacccg gttcttccag taatttgcgc
     8581 aagcggcgat tcttaggcat cccttgagaa gcgataaata actggcgtgc aaattcagaa
     8641 taatctttct ccggaatgga ttctggcgaa tctggaaaac gactcaggat ttccgttatt
     8701 ttatcgcgtt gataacgcac aattgtaccg acggctgggt tgagttcgtc aaatttttca
     8761 tgccttgaac tctgaaccgg tcccgaaatt ataagtggtg tgcgcgcttc atcaattaaa
     8821 atattgtcaa cttcgtcaac aatagcataa taatagctac gttgaacttg ctcttcagcc
     8881 gaaagagcca tattatcacg caagtaatca aaaccaaatt cattattagt tccgtaagta
     8941 atatcgcaag cgtacatttt cttacgctct tcggaatcca tcgaattaag aatacagcca
     9001 accgtcagac caagctcggt atagattcgt cccatccact cactatcacg ctgggctaaa
     9061 taatcattga ccgtaacgat atggactccg cgaccagtca gggcattcaa aaaaaccggc
     9121 attgtcgcca ccagagtttt accttcgccg gttttcattt cggctatttt accctgatgt
     9181 aaaacaatcg ccccaatcaa ttgaacatca taaggaacca tatcccagac tatcttctgg
     9241 tcagtgacta accattcttg ccccaccagc cgtcggcaaa cttcttttac catggcaaag
     9301 gcttccggca aaattgcatt tagagcgttt tgctcggcgg tttggatgag tttttgggct
     9361 tcctcttctg agattccaga ttcttcggct tcgattcggg ctgcctcacg agcagtttga
     9421 atttgaactt taagctcagc tgtgcgttcg cgcaattttt ctattggttg ctcagacagg
     9481 gttgcataaa tacgattaat ttctgctacg agcgggagaa tttttttgat ctcacggtca
     9541 gaagaggttc caaaaatttt cgttaagtaa gtagctattc ccattttatc ttaccaaatt
     9601 atgtttattg tatttgacta aaacactcaa aatttgtttc cggaaatttg tcgagttttc
     9661 cgaaactatt cttctcgagt agctgacttg gtttccgagc gcttcaattt ataagttaaa
     9721 gtatcgctaa tatcgtgcag gaccgcatcc aaaatcccat taacaaaggc actactatcg
     9781 tcggtagaat atattttggc aatttcaata gcttccgaaa tagagacttt cggtggaata
     9841 tcattgaaat atttaaattc actaatcgcc attcgaatta ccagacggtc aatgatggca
     9901 atccgttcaa aatcccaatt ttctgatttt tctctaatga tttcatccag ttcgtcacgg
     9961 tgggtaaccg ccgctatgaa taaatccttg gcaaaagttt ttaactcatc ctgaagttca
    10021 gaattttcaa tcacatcatc aattatctga gctatagggt agctggttaa ctcttccgca
    10081 tataaagctt gcagagccac ttcgcgcgcg gctcgtcttt ctctcatcgt tacaactgat
    10141 ccttaaccat aacaaataac aacgccaaaa tttacagtat ttttggggca atatacaatt
    10201 tttctcacaa ctgaattttc agagcctgcg aaacacgtgc catgactcaa aaatattttt
    10261 gagatttttg ccctctcatc tatccattcg cacaatcgta cctcaagaca aactcataat
    10321 aaatattttg ttagtagaac ttaaacggac ttaaaaacgc ttataagcac ttaagagaac
    10381 tttcagacaa ccgcgcaacc tttacaacta caacacgaac aattggcaaa cagcaaaaaa
    10441 taaggtaaat ttcaaatttg aaatgactat caatgaataa gaaaaaattt ccgcaggcaa
    10501 actaaaatgc cacctaaaaa aattgacaac acccaaatcc caacctccaa tgtggaaaag
    10561 tactatctca gtctctctgg caggcaattc atctatccgc cgacagagaa aatcccagtc
    10621 attcaggtag cgaattttcc gatgctcggt caattgaccg ccctccggtt tttagaatgg
    10681 gtacaaaata atcccgatgg agtaatcgct ctgccaaccg gtaaaactcc cgagcatttt
    10741 atcaagtggg tggggtatta tctaaccaat tggcactcgg ctgaggtcat taaggatctc
    10801 gaaatggtgg aaattgatcc ggcacgccga ccggatatgg cgcgcctgca ttttgttcag
    10861 attgatgaat tttatccgat tgatcccaat cagcagaaca gcttttatta ttacatacaa
    10921 aaatactaca tcaagaattt cggacttgac ccagaaaagg cacttttgat taatctctat
    10981 gaaattggta cagctaataa tctgagtatt gccgaaattt tcggcgatga gaaagccgac
    11041 ttgagtttgc ggacacgctg ggcgaaaaat cccatcgaag aattgcaaaa ggaaaccatc
    11101 gcaagaaccg accaattctg cgtcaactac gagagcaaaa tccgggcatt aggtggaatt
    11161 ggcttctttc tgggcggtat tggaccggat ggccatatcg ctttcaatat tcgaggcagt
    11221 gatcattatt caacaactcg tctaatggaa accaactacg aaacccaagc cgccgcggct
    11281 agcgatttag gcggcatcga aatagctcgc aaacgtctgg tgattacgat tggcctcgat
    11341 actattacct ataataaaga tgccaccgca attatcatcg ccgccggtca agctaaagcc
    11401 aatattgtgc gcgattctat ccaaaatttt ccttcaatcc agtatccagc caccgttttg
    11461 caaaaattac ctaacgcccg cttctacttg acggaaggcg cagccagcaa actgttcgag
    11521 agacgatatg aaaaaatggc aaagtcgccc gaattggagc cgaaaaccat cgagaaagca
    11581 gtcattaatc tcactctcga aaaaaataaa aaacttaccg acttaaccct cgcggatttt
    11641 aactctaacc gatttaccga attgattctt aagcgtacga atcagaccca taccgaaatc
    11701 tgtagaaatt tacacgagtc tattatcaac cggatagatc gtggcattta ttatttagac
    11761 aacgaagtct ttatgcacac ttcaccacat cacgacgatg aaatgctcgg ttacctccct
    11821 tatattgttc atctcgtgag aaatccacgt aacaaacatt atttcaccta tatgaccagt
    11881 ggattcaccg ccgtgactaa ttcctatgta ctggaacgat tgaaaaatct acaagatttg
    11941 gtagatagcc ctactttcca ggttttaatg caggaacact atttcgaccc cgccaacacc
    12001 gccgcccgta accgggacgt caatttgtat ctggacgggg ttgcccttta tgatgttaag
    12061 aaaaaaactg aagccgaatc gcgccgacta ttatccattt tgactgaaat ctatgaagaa
    12121 aataacgtca tctacctcaa agaccgcatt agcgaattaa tcaattattg cgcaacccaa
    12181 taccccggta aaaaagacat cgcgcacatt cagaaattaa agggaatgat tcgagaatgg
    12241 gaagctgatg ttctctgggg ctattttggt tttaatagca ataatgtctt tcaccttcgt
    12301 ttaggttttt ataaaggcga tattttttcg gaagaaccga cgttggatcg cgatgtcctt
    12361 cctatttacc aattaatgaa aaaaataaga ccgaccgtca ttacggtagc ccttgaccca
    12421 gaaggcagtg gtcctgacac gcattacaaa gttctgcaag ccatcactga ggcactcaaa
    12481 ctttatgaaa aagaagaaga tatctctaat ctacgcatct ggggctatcg caacgtctgg
    12541 caccgttttc atcctgccag caccaatatt tacgttccgg tcagcctgaa ctcaatggct
    12601 attatggaaa atgcctttat ggaaagcttc ggctctcaaa gatcggctag ctttcccagc
    12661 tgggagttcg atggaccgtt ctgccggttg gcgcgcaaga ttcaggttga tcaatactta
    12721 aaaatcaaga cttgtctggg acgcgatttt tttatcaaaa atgaacatcc tcgattgcgc
    12781 gctacccacg gatttgtttt catcaaagaa ttgacaccaa aggaatttta tcgtcattct
    12841 gaggagtcgc gccgggtcac cgaaaatcta taatatcgaa tgaaaacaac ttgttattta
    12901 tcaatccggc aagcatcaat aaattccaga agtaggcgga ctatcatttt atttggaatt
    12961 gataagcaat atatttttga ctggcatatt gatctccata taatcagaag atattaatgt
    13021 taattgttct gtgttttatt cgaaaatttc atcggtttca acattttcaa tatgctatta
    13081 atgatggtag tagctggatt aatagctccg atcaacggct tgggaataaa gtcatttgaa
    13141 ttgcttttta tggctatcct ctcgttagca atttatcca
//
LOCUS       DAOMJU010000052        13029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_4795_length_13029_cov_21.552798, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000052 DAOMJU010000000
VERSION     DAOMJU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13029
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4795_length_13029_cov_21.552798"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1362)
                     /locus_tag="PLO58_06185"
     CDS             complement(37..1362)
                     /locus_tag="PLO58_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74412.1"
                     /translation="MTSNNDLKSKNAMSIILLLTAIFFAMSCRECPTESENNIDLIVK
                     DIFCTSVVLKISLPYSGAVNTFALDRNDSTVATYTCSEDDTLIIDEGLTPATKYLYSV
                     RFLKDGKTKSESDPVAVHTMNITSHDISYWDIDTLGISGGIYDLWIVSEDNIWAVGQI
                     IAEDPDSSFDGSGEEWFNAAHWDGEKWNLIRILNTATLYSIWYFSESDIWVSSGFPKH
                     WDGQQWTMYHFQDMGLGIEVSTEHIWASSPNNVYFVGWKGSIVHYNGSNFTKMESGTT
                     CKLEDVWGIDGNHIWAVGTESDNSRSVILFYNGKTWNTLHDTNNERKCWSNSVWTDNP
                     YLLFLNGGSGRYLFDLMQEKLSKYESAGRWFGYDIYGTAYNDIFASTAGSEMLHYNGS
                     TWYLYPEVKAQFEDYFRIRAVKANDEIVVASGYYYTGIGGLPIIIRGYR"
     gene            complement(1642..2751)
                     /locus_tag="PLO58_06190"
     CDS             complement(1642..2751)
                     /locus_tag="PLO58_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74413.1"
                     /translation="MLRRIEFKIVLLGLLAWVLLLNPVNLLAIGDKYAGEFLKFGLGV
                     REMALGGAVVSDPTAVAAHYWNPATLTDNTCFSGNFMHTEEFAGAVALDHFALALPGK
                     GEYSFGAGYLRIGVDDIADTRNALLDLGTDGLGPGDEGYLGPDADGTEGNGRLDPGER
                     LDYGKFGSFGASENAVFLSGAKILQPKLKVGGTLKMLYRSLGDDKAYGIGFDIGVLYQ
                     PAPKINLAAAFNDLTTTFMLWNDGEREIVAPSLRIGGSVHHHFKKLPLAIEPLFGADI
                     LFEGKQNTADISIGGTVVRARIGLEVIYKECIALRAGRDDLGNPQIGLGLNTSVGSLD
                     YGLGLGGSYAEFGSSHRIALTLHFAELQRALKRYL"
     gene            complement(2809..5424)
                     /gene="mutS"
                     /locus_tag="PLO58_06195"
     CDS             complement(2809..5424)
                     /gene="mutS"
                     /locus_tag="PLO58_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQO74414.1"
                     /translation="MTKSTEKIDETTPVMRQYNAVKAQYPDALVLFRMGDFYETFRED
                     AKQAAKILNITLTKRANGKAAAVPLAGFPYHALESYLHKLLKAGLKVAICEQVEDPKL
                     AKGVVKRAVVEIITPGTAISDRFLEKEKNNYLVSVYINGENAGLAALDISTGEFYLSE
                     IPTARITEAVLDLHPAEIICPESIQKEIQTLFTNQVLRITPLEDWIFTFETAYQTLIE
                     HFKTDSLKGFGCQSAVHGITAAGPILHYLRQNYQTNLQHIYSIRNLATDETVLIDDFT
                     RRNLEIFQTMQSGDRKGTLMDVIDQTVSPMGVRLLTKWLIRPLRDPAAINQRLDGVEF
                     LYNNTKIRHQLREMLAHCGDLERLISRLSTNRASAREIVALKNTLKLIPGIRDLLPNQ
                     LAFSEINSLLNPLPELVELLERAIKDEDLPLSIHEGGFIKDGYSAELDEYRHIARHGK
                     EWIARLQETERQKLGIPSLKVGYNKVFGYYIEVTRTHAEKIPPEYIRKQTLVNNERFV
                     TPELKEYEEKLLNAEERINQIEFELFQDLRTKILQFIEAIQKNAGALAQIDVLAGLAE
                     LAEKNRYCRPVINDGTAIDIKAGRHPVVEHLLPPGEKFIANDLHIDNLSEQILIITGP
                     NMAGKSTYLRQIGLIVLMAQIGSFVPADSATIGVVDKIFTRVGASDNLAAGESTFLME
                     MHETANILNNVTPHSLVLLDEIGRGTSTYDGMAIAWSVTEYLHNQPNVAAKTLFATHY
                     HELTELEKILPRLKNYNVAVREYGDRVIFLRKIVPGGCDKSYGIHVAQMAGVPPEVIG
                     RAREILTNLSPEEKVLPTNREKFRHLPEKDANQLGLFGDDLKLKKMLAEVDIDNLTPL
                     EALQKLAEIKKSLGG"
     gene            complement(5595..7787)
                     /gene="feoB"
                     /locus_tag="PLO58_06200"
     CDS             complement(5595..7787)
                     /gene="feoB"
                     /locus_tag="PLO58_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQO74415.1"
                     /translation="MNFPPHRHRHRRGQRQISPDSSQKVIRIALAGNPNSGKTSLFNA
                     ITGQHQHIGNYPGVTVEKKYSLFHYKDDVIEIIDLPGTYSLTAYSLEEIVARDFVIKE
                     KPDVIIDVLDATNLERQLYLLLQFQELGVPLVGALNMSDECEKKGIDIDHERLSSILG
                     IPLVKTVGTRGYGIDCLLDTAIRVAKGKLRADQRHLNYGEDVENAHDQIIKVLETDPD
                     FSHKYPVHWLAIKLLENDRDAIQKVKSEHTNAETVLGESERIRRQLENHFHDDIISIL
                     AERRYAYINGAIREAVRIDRTRPAIDWTERIDRVILNRFLGIPIFLLIMFLIYQATFG
                     LGNPLAGLISNSFANLADFVNLHLPDNRFRDFLTNGLIAGVGGVLVFLPLVVLLMLGL
                     SLLEDTGYMARAAFVMDKFFHLFGLHGRSFIPLMLATGCAVPGVMAARSLANRRDRII
                     TIMVTPLMMCSAKTPVIAMLAAAFFPEKAGLIFWGIWFLSWFMALIIALIFRKTFFKG
                     DQSPFVMELPPYRWPSFRSVMIHMWQRTLAYLRKAGTIILFAAIIVWFALHNPTNPGS
                     HSTDASQFNVNNNQGSETVNQGQQLYHSYAGQIGRGLEPILKWAGFDWKIGVSLIAGL
                     AAKEVIISTLGMLYGIPEGAQEATEQSKMTTVSYQISRDPNYSPLSAFSLMIFVMLYT
                     PCLATLAVVRKELGHIKWALFQAVYTVTIAFIASVAIFQIGTFLQHIF"
     gene            complement(7784..8041)
                     /locus_tag="PLO58_06205"
     CDS             complement(7784..8041)
                     /locus_tag="PLO58_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HQO74416.1"
                     /translation="MSTNSTTLVARSLAEVPAGKCFRIKKVHGHGEIRQRLIDLGFIK
                     NEVGTVIREALLKDPIEVELKGTHFSLRRSEARLIEVEVID"
     gene            complement(8044..8817)
                     /locus_tag="PLO58_06210"
     CDS             complement(8044..8817)
                     /locus_tag="PLO58_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HQO74417.1"
                     /translation="MNEKQSHGPTISQAQEDYLKIILELINSRRVARIKEIAQRKNVS
                     MPTVTEAMRKMAQDGLINYSAHEFIDLTSEGEQAAHQMTARHKFLRLFLEDILNIPSE
                     TADTEACLLEHHLSPITLERMILLYQFLVNCPHHDSNLIEDFRQCLSAEKLADKTSCR
                     DCFVKIAFPHTVTNPFAVHSLLANLSEGQKGTVTMLGGNTAMRHKLIEQGLVPGATVQ
                     MERTGNKSQPFVISVNGLQIKLDIEQANLIEVAISSGEI"
     gene            9112..9393
                     /locus_tag="PLO58_06215"
     CDS             9112..9393
                     /locus_tag="PLO58_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74418.1"
                     /translation="MSDNFNNSGKKGSAQPYDSKINEQCPKCSGNIVYRKSTYFGNKV
                     YYKVCNNCGWYITLPKEVWKDIISASLKPAKATPSESAEQDEQIEAIVS"
     gene            9485..10147
                     /locus_tag="PLO58_06220"
     CDS             9485..10147
                     /locus_tag="PLO58_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74419.1"
                     /translation="MVLTGSKSRDVILIGAAFKAEINPLIEKLSQASFSPTGIDFDYI
                     LTGSGLERVRCCLAEKVVCQDYLAIINIGTAGVLNDTCPLMSIFYPHRFCTLSNDHLT
                     YLTLPNDFLESSHFLSTGWKSGTLFSSREPVTSAAKRWAILQNSQADVVDMEAFAYAD
                     FCQRHKIPFACLKVITDRADAEVKQNFNANLAESAQLLAQNALIFVDFLLNKLDIKTE
                     NG"
     gene            10140..11003
                     /locus_tag="PLO58_06225"
     CDS             10140..11003
                     /locus_tag="PLO58_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO74420.1"
                     /translation="MDDPQKISVIIPTYNRETCLERAIVSVLSQTYRNFELIVIDDGS
                     TDNTSRIIHKYKNKIRYFSQLHSGVSTARNLGLEKSEGTWVAFLDSDDYWLPHKLERQ
                     MQCLAENPQWLVMQTDEKWIRNGVVVNPTKKHQKYSGWIFVQCLPLCIVSPSAVIVHQ
                     KVFNDVGVFDESLPVCEDYDLWLRVSLKYEIGLLPEKLIIKTGGHSDQLSRKYWGIDR
                     YRVLALEKILNSELDRQQTELVLDEIIKKLTILSKGRTKHPELPNIYADKLSDYQKKK
                     EVFLANQKNCY"
     gene            10978..11925
                     /locus_tag="PLO58_06230"
     CDS             10978..11925
                     /locus_tag="PLO58_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74421.1"
                     /translation="MQIKKIAIEQIPSQSQERLFVEEINPDCNKPNESLFLQPVFLHH
                     TRDGFTILYGFNYISNIYQRGGKNIQAFVIPKSQSLFSALNIVADFIRRSRPLWPIEI
                     ARILQLFNQQQFSNAKKSAFFKKFTEKDLTRTLEERYLALFGLNPLLHKFLIEKKAPL
                     KTWFLTAEYPPVIRDLILNLITECQPSLSTFEEISRNLLEILRREDIAPEQLTAEMSQ
                     VLIEKAEEIQPSQKLEIFRSKIQERRFPQMTIHRERLNNFLQTIQLPSAVEIEFNPNF
                     EERGYRLKAEIRTPNDLRELTEFLNSAAQSDLKHLLEIL"
     gene            11945..>13029
                     /locus_tag="PLO58_06235"
     CDS             11945..>13029
                     /locus_tag="PLO58_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74422.1"
                     /translation="MNLPADRLIKKIFVTAECVRDEIVEQIFTAYPKQKKIAIESEKS
                     LTETIPQEDPKKILLVTRSKGEVVKDCPGTAAPYLCCRYQVINQTLNCPLNCTYCILQ
                     YYLNQPATIIYTDYQKIIAEIEQKIASQSHRLLRFGTGELGDSLALTASQIFAIKLIG
                     YFATQKNALLELKTKTDRIGLILDQPHNGHTVISWSLNPLVIRNSEEHLAPPPTRRLK
                     AAAEAARRGFLIGFHFDPIIDIPDWESQYLDLIEELYHYVDPARIAWISLGSLRFPPS
                     MKTKIIARYPYSRLPYGELIRGQDQKMRYPRPRRTEIYRSIYRKLTEIREPPFVYFCM
                     ESPTVWQDVCGFAPQSNAHLDYLFAESL"
BASE COUNT         3655 a         2850 c         2866 g         3658 t
ORIGIN      
        1 tttttcattt tttaaatcct cttaattaat aggttttcag cgataccctc tgataatgat
       61 tggaagacct ccaatgccgg tgtaataata tccactagcg actactattt catcgttggc
      121 tttaaccgcc cttatccgaa aataatcttc aaattgtgct tttacttcag gataaagata
      181 ccatgtcgag ccattataat gtaacatttc actgccagca gttgaagcga agatatcatt
      241 ataagcagtt ccataaatat cataaccgaa ccaccttcca gcgctttcat atttactcaa
      301 tttttcttgc atcagatcaa ataaatatct gccactacca ccattgagaa aaagtaagta
      361 cgggttatca gtccacaccg aatttgacca gcattttctt tcattattgg tatcatgtaa
      421 agtattccac gtcttgccat tataaaaaag gataacactt cggctgttat cagattctgt
      481 tcccaccgcc cagatgtggt taccatctat tccccagaca tcttccagct tgcaagtcgt
      541 tccgctttcc atcttagtaa agttcgaacc attataatga acaatagagc ctttccagcc
      601 aacaaagtag acattattag gagatgaggc ccatatatgt tcagtgctta cttctatacc
      661 caatcccata tcttggaaat gatacattgt ccattgttgt ccatcccaat gtttggggaa
      721 accagagctt acccagatat cactttcaga aaagtaccat atactatata gagtcgccgt
      781 atttaatatc ctaatcagat tccatttttc accgtcccag tgcgccgcgt tgaaccattc
      841 ttctccggag ccgtcaaagg aagaatccgg atcttcagca atgatctgac cgaccgccca
      901 gatattatct tccgagacaa tccacagatc atatatgccg cccgaaatcc ctagggtatc
      961 gatgtcccaa tacgaaatgt cgtggctggt gatattcata gtatgcaccg cgaccggatc
     1021 ggattcagac ttggtcttgc catctttcaa aaaacggacg ctatataaat attttgtagc
     1081 cggagttaag ccttcatcaa taatcagggt atcatcctca gaacaggtat aagtcgctac
     1141 agtcgagtca ttccggtcga gcgcaaaggt gttaacagcc ccggagtaag gtaaacttat
     1201 tttcagaact actgaggtgc agaagatatc tttaacaatc aaatcaatgt tattttcaga
     1261 ttcagtcgga cattcgcggc aggacatagc aaaaaagatg gcggttagca gtaagatgat
     1321 tgacattgca tttttagatt ttaggtcgtt gttagaggtc atcgtttttc cctttttgtt
     1381 cttttcacaa ttgaaaatac actcatttta gcgtaatttc aaccattgtg tgtttccgcc
     1441 actggcgaat agctcccgcg cccggactgc tgatacagtc agacgcgggt atgtacagga
     1501 attttgagtt gcatattccc ctccgttagt tgtatgttct tgcgtctcaa catggtagcg
     1561 ttgagacgaa gggaaaaatt ataactataa ctaataaatt aggacaatcc tgtcaatctc
     1621 gttatccgtg tcagaaaatt tttacaggta gcgcttgagg gcgcgttgca attcggcgaa
     1681 gtggagcgtc aacgctatgc ggtgcgagga accgaattcg gcgtacgagc cgcccagccc
     1741 cagcccataa tctaaactgc cgacggaagt attgagtccg agaccaattt gaggattgcc
     1801 aagatcatcg cggccagccc gcaaagctat acattcttta taaattactt ccagtccgat
     1861 ccgcgcccgt acaaccgtac cgccgataga tatatccgcc gtattttgtt taccctcgaa
     1921 caaaatatca gcaccaaata gaggttcaat tgccagcggt aattttttaa aatgatgatg
     1981 aaccgagccg ccaatccgca aacttggcgc gacgatttcg cgctcaccgt cgttccagag
     2041 cataaatgtt gtcgttaagt cattgaacgc cgccgctaga ttgattttag gcgccggctg
     2101 ataaagtacg ccgatatcaa agccaattcc ataggcttta tcatcgccca gcgaacgata
     2161 gagcatcttg agagtgccgc caacttttaa tttaggttgc aaaattttgg ctccggaaag
     2221 aaaaaccgcg ttttcgctgg cgccgaacga accgaattta ccgtagtcaa ggcgctcgcc
     2281 gggatcgagt ctaccattgc cttccgtacc gtcggcatcc ggtcccaggt agccttcatc
     2341 acccggtcct aaaccatcag ttccaagatc gagtaaggcg ttgcgcgtat cggcaatatc
     2401 atcaacccca atccgcaaat agccggcgcc gaaggaatat tcgcccttac cgggtaatgc
     2461 cagcgcaaaa tgatcgagcg caaccgcacc ggcgaattct tcggtatgca taaaattgcc
     2521 cgagaaacaa gtgttgtcag tcaaggtagc tggattccag taatgcgccg ccacggcggt
     2581 cggatcagat acaaccgcac ctcctaacgc catttcccgc acacccaagc cgaatttgag
     2641 gaattctccg gcatatttat cgccgatggc taagagattt accggattta acaataaaac
     2701 ccaagccagc agaccgagca ggactatttt aaattcaatt cttctcagca taaaattgca
     2761 attaatacgg ccgtaatgcg cggaatgttc cctgaattgc aattgcattc aaccacccaa
     2821 gctctttttg atttcagcga gtttttgcaa agcctccagc ggcgtgaggt tatcaatatc
     2881 aacttctgcc agcatttttt tcaatttgag gtcgtcaccg aacaacccga gctggttggc
     2941 atctttttcc ggcaaatggc ggaatttctc gcgattggta ggtaaaacct tttcctcagg
     3001 actcaaatta gttaaaattt ctcgggcgcg gccgatgact tccggcggaa cgccagccat
     3061 ttgagcgacg tgaatgccgt aacttttatc gcaaccgccg ggaacaattt tacgcaggaa
     3121 aatgacccga tcgccgtatt cccgtacggc tacattatag tttttcaggc gtggtaagat
     3181 tttttccaat tcagtcaatt cgtggtaatg agtggcaaat aatgtcttgg cggcgacgtt
     3241 gggttgattg tgtaaatact cggtaaccga ccaggcgatt gccataccgt cataagtcga
     3301 ggtgccgcgg ccaatttcgt ccagcaatac cagtgaatgc ggcgtcacat tattgagaat
     3361 attggcggtc tcgtgcattt ccatcagaaa agtggattca ccggcggcta aattatcact
     3421 ggcaccgacg cgagtgaaga ttttgtcaac tacgccaatc gtggcggaat cggctggcac
     3481 aaagctaccg atttgcgcca ttaaaacgat tagcccaatc tggcgcaggt aagtagattt
     3541 gccagccata tttggtccgg tgataattaa aatttgttcg ctgagattgt caatgtggag
     3601 gtcgttggcg atgaatttct cgcccggcgg tagcagatgc tctacaactg gatgacgtcc
     3661 ggctttgata tcaatcgctg tcccgtcatt gatgaccggg cgacagtaac gatttttttc
     3721 cgccagttct gccagtccgg ctaaaacatc aatttgggct aacgcgccgg cgtttttttg
     3781 gattgcttcg ataaactgta aaatcttggt gcgcaaatcc tggaataact cgaattcgat
     3841 ctgattgatg cgctcctcag cgttcaacag tttttcctca tattctttca attcaggagt
     3901 gacaaagcgc tcattattga ccagtgtttg tttacggata tattccggag ggattttttc
     3961 ggcgtgcgta cgggtgactt caatgtaata gccgaagact ttgttgtagc cgacttttag
     4021 cgagggaata ccgagtttct ggcgttcggt ttcctgcaag cgagcgatcc actctttgcc
     4081 gtggcgggcg atatgacgat attcatctaa ttccgccgaa tagccgtctt taataaatcc
     4141 gccttcgtgg atgcttagag gtaaatcctc gtccttaatg gcgcgctcta agagttcgac
     4201 caattccggt agtggattga gaaggctgtt aatttccgaa aaagccagtt gattcggaag
     4261 aaggtcacgg ataccgggaa tgagttttaa ggtatttttt aaagcgacaa tttcccgagc
     4321 ggaagcgcgg ttggtgctaa ggcgcgagat caggcgctct aagtcaccac aatgcgctaa
     4381 catttcgcgc aattggtggc ggattttggt attattgtat aaaaattcga ccccgtcgag
     4441 tcgttggttt atggcggctg gatcacgcaa tggccgaatc aaccatttgg ttaacaaacg
     4501 cacacccatg ggactgacag tctggtcaat gacgtccatt agcgtacctt ttctatcgcc
     4561 gctttgcata gtctggaaaa tttccagatt acgccgggtg aaatcgtcaa tcaggactgt
     4621 ctcgtcggtc gccagattgc gaatagaata tatatgctgg agattggtct ggtaattttg
     4681 gcgcaaatag tgcaaaatcg ggccggcggc ggtaatgccg tgcaccgcag attgacaacc
     4741 aaaacctttg agcgaatcgg ttttaaagtg ttcgattaga gtttgataag cagtttcgaa
     4801 ggtgaaaatc caatcctcga gcggagtgat gcgcaaaacc tgattggtga acagagtttg
     4861 gatttctttt tggattgatt ctggacagat gatttcagcc gggtggagat cgagcacggc
     4921 ttccgttatt ctggcagtcg ggatttcgct cagataaaat tcaccagtgg agatgtccag
     4981 agcggcaagt ccggcatttt ctccattaat ataaacggag acgaggtaat tgttcttttc
     5041 tttttcgagg aagcgatcgg aaatagccgt gccgggagtg atgatttcga ccacagcgcg
     5101 cttgactacc cctttagcca gtttgggatc ctcgacctgc tcacagatag cgactttcag
     5161 gccggctttc agtaatttgt gtaaatatga ctcaagggcg tggtaaggaa atcctgccag
     5221 cggaacagcc gcggctttgc cattggcgcg cttagttaga gtaatattga gtattttggc
     5281 ggcttgttta gcgtcttcgc ggaaagtctc gtagaaatca cccatgcgga aaagcaccag
     5341 cgcgtccgga tattgagcct tgacggcgtt atattgacgc ataactggag tggtttcgtc
     5401 aattttttct gtagatttag tcatcgcatt tcatttccga ttgaaatata cagaaagtaa
     5461 cgcaatgttg aaaacgggaa aatcagagtt tatctcacta aaaatcgccg gtttttccga
     5521 acaaagttga ataattaaga gtaagctgaa agaatttttt tcaagacagc ttgtttttac
     5581 cgaaaacatt gatatcaaaa tatgtgttgc agaaatgtgc ctatctggaa aatagcaaca
     5641 gatgcaatga aggcaatcgt tactgtataa acggcttgaa aaagagccca ttttatgtgg
     5701 cctaattctt tcctgaccac cgccaaagtc gctaggcaag gggtatagag cattacgaaa
     5761 atcattaaac tgaaagccga tagaggtgaa taatttggat cccggctaat ttgataactt
     5821 acggtggtca ttttagattg ttcagttgct tcctgggctc cttctgggat gccgtaaagc
     5881 attccgagag tggaaataat aacttccttt gccgccagac cggcgattaa actgactccg
     5941 attttccagt caaatccagc ccattttaga attggttcga gtccgcgacc aatttgcccg
     6001 gcgtaactat gatataattg ctgaccctga ttaactgttt cactaccctg attattattt
     6061 acattaaatt ggctggcatc ggtagagtgt gaacccgggt ttgtcggatt atgaagtgcg
     6121 aaccagacaa taatggcggc aaataaaata atcgtcccgg cttttcgtag gtaggctaag
     6181 gttctctgcc acatgtgaat catcacgctc cgaaaagaag gccagcggta aggaggcagt
     6241 tccatcacga aaggactttg atcgccctta aagaaagttt tgcggaaaat aagagcaatg
     6301 attagagcca taaaccagct aagaaaccag atgccccaga aaattagccc ggctttttcg
     6361 gggaaaaaag ccgccgccag catggcgatt accggggttt tagcactaca catcatcaat
     6421 ggtgttacca taatggtgat aattcggtcg cgtcgattag ccaacgaacg agcggccatt
     6481 accccgggaa ctgcacagcc ggtcgctaac ataagcggaa taaaactccg accgtgcaaa
     6541 ccgaataaat ggaaaaattt atccatcacg aaagccgctc gagccatata accggtatct
     6601 tccagcagag aaagtcccaa cattaataat acaactaatg gcaaaaaaac cagcacgcct
     6661 cccacacctg ctattaaacc gtttgtcaaa aaatcacgaa accggttatc tggtaaatga
     6721 aggttaacga aatctgccag gttagcgaag ctattcgata tcaaaccagc taagggattt
     6781 cccaatccga aagttgcttg gtatatcaaa aacataatca gcagaaaaat cgggatcccc
     6841 agaaaccgat tgagtataac ccgatcaatt cgttcagtcc aatcaattgc cggccgagtt
     6901 cgatcaatcc gcactgcctc gcggatagcg ccattgatgt aggcgtaacg tctttcagct
     6961 aagatcgaga tgatatcgtc gtggaaatga ttttcgagtt gacgtcgaat gcgttcgctt
     7021 tcgcctaaaa cagtttcagc attagtatgt tcgcttttga ccttttgaat ggcatcgcgg
     7081 tcattttcca gcagtttgat agccagccag tggaccgggt atttatgaga aaaatccgga
     7141 tcggtttcca gcaccttgat aatctgatcg tgggcatttt caacatcttc gccgtagttt
     7201 agatggcgct gatcagcccg gagcttgcct ttggcaactc gaatagcggt atccaaaaga
     7261 caatctatac catagccgcg ggtaccaact gttttaacga gtggaatccc cagaattgac
     7321 gaaagtcgtt catggtcaat atctataccc tttttttcac attcgtccga catatttaga
     7381 gcaccaacta agggcacacc gagttcctga aattgtaaaa gcaggtacag ttgtcgttcc
     7441 aagttagtcg cgtccagcac atcaatgatg acatccggtt tttctttaat gacgaaatcg
     7501 cgcgcaacga tttcttccaa cgagtaggcc gtcaaactgt aggtgccggg cagatcaata
     7561 atttcgatta catcgtcttt atagtggaaa aggctgtatt ttttctcaac ggtaaccccc
     7621 ggataattcc cgatgtgttg atgctggccg gtaatggcat tgaaaagtga agttttacct
     7681 gaattcggat tgccggctaa tgcaattcgg attactttct gggaactgtc tggtgaaatt
     7741 tggcgctgac cgcggcgatg gcggtgtcgg tgtgggggaa aattcaatct atgacctcga
     7801 cctcgatcaa acgggcttca ctgcgccgta gtgaaaagtg agtgcccttg agctcgacct
     7861 cgatcggatc ttttagcaaa gcttcacgga taaccgttcc aacctcattt ttaatgaaac
     7921 caaggtcaat cagacgttgt ctgatttcac cgtgaccatg gacttttttt attctgaaac
     7981 atttgccggc gggaacttca gctaggctcc gggctacaag agtagtcgaa tttgtggaca
     8041 ttattaaatt tctcctgaag atattgcgac ttcgataagg ttagcctgtt ctatatcgag
     8101 ctttatttga agaccgttga ctgaaataac aaaaggctgg ctcttattac cggttctttc
     8161 catttgaacc gtagcgccgg ggacgagccc ttgttcaatt aatttatgtc gcatagctgt
     8221 attcccaccg agcatcgtta ccgtcccttt ctgtccttcc gatagattgg ctaatagcga
     8281 atgaactgca aaaggattag taacggtatg gggaaatgcg attttgacaa aacaatcacg
     8341 acaactggtt ttatccgcaa gcttttccgc agacagacac tgcctaaaat cttcgatgag
     8401 gttgctatcg tgatgaggac agttcactaa aaactgatag agtaaaatca tcctctctaa
     8461 ggttattgga ctaaggtgat gctcgaggag acaggcttcg gtgtcggcag tttcagatgg
     8521 gatgttcagt atatcctcaa ggaaaagacg aagaaattta tgccgggcgg tcatctggtg
     8581 agcggcttgt tcaccctcgg aggtaaggtc aataaattca tgcgcggagt aatttatcaa
     8641 gccgtcctgc gccattttcc gcatagcttc ggtcacggtc ggcatactaa cgtttttccg
     8701 ctgggcaatt tctttaatgc gggcgacgcg gcgggaatta atcagctcga ggataatttt
     8761 gagataatct tcctgcgctt gggagatggt aggtccgtga gattgttttt cattcatact
     8821 gcctaattta attaggttaa cctaactaca caatactttt tggagaaaat attctattgg
     8881 aaaaaaataa attgactact attcgtttct gtgaacaaaa tgaattatag ctcgtaaaag
     8941 gggcgaatca gtaatttttt gttagtttag aaattaaatg cttgacaatt accattagaa
     9001 atacgaatat ttagaccagt agagaataag aataaaaaat taatttttta ctgattttta
     9061 gtagtaaatg tcaaatgtcc gaagaaacca aatttcaaag gttcaatcac tatgagtgat
     9121 aattttaaca acagtgggaa aaagggaagc gcccaacctt acgacagcaa gattaatgag
     9181 caatgcccta aatgttccgg caacatcgtc tatcggaaaa gcacttactt tggcaataag
     9241 gtttattaca aagtgtgtaa taattgcggc tggtatataa ccttacccaa agaagtctgg
     9301 aaggatatta tcagtgccag tttaaaaccg gctaaggcta ctccgagtga atctgccgaa
     9361 caagatgaac agattgaggc gattgtttcc taatttggtc tgattcccca attaattttt
     9421 aaaatattcc caatattagg tggaaaattt ccaaaattga ccacaaggcg ttaatattgt
     9481 atcaatggtc ctaactggtt caaaatcgcg agatgtaatt ctgataggtg ccgcttttaa
     9541 agccgagatt aacccattaa tcgaaaaact cagccaggct tcctttagtc caacggggat
     9601 agattttgat tatatcctaa ctggttctgg cttggaaaga gtaaggtgct gtttggcgga
     9661 aaaagtcgtt tgtcaagatt acctagctat cattaatatc ggtaccgctg gtgtcctcaa
     9721 cgatacttgt ccgctaatga gtatctttta tccgcatcgg ttttgcacat tgagcaacga
     9781 ccatttgacc tatcttacac tgccaaatga tttcctcgaa tctagtcatt ttctgtcaac
     9841 tggctggaaa agtggaacgc tattttcgag tagggagccg gtaacttcag ccgcgaagcg
     9901 ctgggcaatt ttacagaatt ctcaggctga cgttgttgat atggaagcct ttgcctatgc
     9961 tgatttttgc cagcggcata aaattccttt tgcctgcctg aaagtcatta cggatcgggc
    10021 agatgccgag gtgaagcaaa atttcaatgc caatcttgcc gagtcagctc aattgctggc
    10081 gcaaaatgca ctaatttttg ttgatttctt gctgaataaa cttgatatta agactgaaaa
    10141 tggatgaccc gcagaaaatt tctgtcataa tcccaactta taatcgggaa acctgcctgg
    10201 aacgcgccat tgtttcggtt ttgagtcaga cttaccgcaa ttttgaatta attgttattg
    10261 acgacggtag tacggataat accagccgca taattcataa gtataaaaat aaaattcgtt
    10321 atttttccca attgcattca ggagtgtcca ctgctcgtaa tcttggactg gaaaaatccg
    10381 aaggcacctg ggtagcgttt ctcgattctg atgattattg gttgccgcac aaacttgagc
    10441 gtcagatgca atgtttggcg gaaaatccgc agtggttggt gatgcaaacc gacgagaaat
    10501 ggattcggaa tggggtcgta gtcaatccga caaagaagca tcaaaaatat tccggttgga
    10561 tttttgtcca atgtctgccg ctttgtatcg tcagcccttc cgcggttatc gttcatcaga
    10621 aggtttttaa tgatgtcgga gtatttgatg aaagtttacc ggtttgcgaa gattatgatc
    10681 tctggttgcg agtttccttg aaatatgaaa ttggattact accggaaaag ttaatcatta
    10741 aaactggtgg tcattccgac cagctttcgc gtaaatactg gggcattgat cgttatcgag
    10801 tgctggcatt ggagaaaatt ttaaactccg aactcgaccg gcaacaaact gaattggtac
    10861 tcgatgaaat cattaaaaaa cttacgatct tgagcaaagg acgcactaaa catcccgagt
    10921 tgcctaatat ttatgccgac aaactatctg attaccaaaa gaaaaaggaa gtttttcttg
    10981 caaatcaaaa aaattgctat tgaacaaatc ccgtcacaat ctcaggaaag gttgtttgtg
    11041 gaggaaatta atccggattg taataaaccg aacgaatcgc ttttccttca gccggtattt
    11101 ttacaccaca ctcgcgatgg tttcactatc ctttatggat ttaattatat ttccaatatt
    11161 tatcaacgtg ggggaaaaaa cattcaggcg tttgttattc ctaaaagtca atcgcttttt
    11221 tcagccttga atatagtggc ggattttatt cggcgctcgc gtccgctttg gccaatcgaa
    11281 attgcgcgga ttttacagct atttaatcaa cagcaatttt ccaatgccaa gaaatcggca
    11341 ttttttaaaa aatttaccga gaaagatttg acccgtactc tggaagaacg ctatttggca
    11401 ttgtttgggc tcaatccact tttgcataag tttttaattg aaaaaaaagc gccattgaaa
    11461 acctggtttt taacggctga gtacccgccg gtaattcgag atttgatcct gaatttaatt
    11521 actgaatgcc agccgtcgtt gagcactttt gaagaaatta gccgtaattt gctggaaatt
    11581 ctccgccgcg aggacattgc tcctgaacag ttgacagcag aaatgagcca agttttaata
    11641 gaaaaagccg aggaaattca accgtcacaa aaacttgaga tattccgtag taaaattcag
    11701 gaacgccgtt ttccacagat gactatccat cgggagcggt taaataattt tctgcaaaca
    11761 attcaattgc catcggcagt cgagattgaa tttaacccga attttgaaga acgaggttac
    11821 cgacttaagg cagaaattcg cacgccaaac gatctgcggg aacttactga atttttgaat
    11881 agtgccgctc aatccgatct gaagcattta ctggaaatac tgtaaatagt gaactgcaat
    11941 tccgatgaac ctgccggctg atcgcttgat aaagaaaata ttcgttacgg cggaatgtgt
    12001 tcgagatgaa atagtcgagc aaatttttac tgcttaccct aaacaaaaaa agatagcaat
    12061 agaatcggaa aaatctttaa ctgaaacgat tccgcaggaa gaccccaaaa aaatattact
    12121 ggtaactcgt tctaagggcg aagtagttaa ggattgtccg ggtacagccg ctccgtatct
    12181 ctgctgtcgc tatcaggtaa ttaatcaaac cttaaattgc ccgttgaatt gcacttattg
    12241 tattttgcaa tattacctga atcagccggc aacaattatt tataccgatt accaaaagat
    12301 aattgctgag attgaacaaa aaattgccag tcaatcccat cgtttattgc gcttcgggac
    12361 cggcgaactc ggcgatagtt tagctttgac cgcctcccaa atttttgcca ttaagttgat
    12421 aggatacttt gccactcaga agaatgcttt gctcgagctt aaaaccaaaa ccgatcgaat
    12481 cggtttaata ttagatcagc cgcataatgg tcacacggtc atttcctggt cgctaaatcc
    12541 attagtcata agaaatagcg aagagcactt agcgcctcca cctacaaggc gcctaaaagc
    12601 cgccgccgaa gccgctcggc gaggtttcct aattggtttt cattttgatc cgatcattga
    12661 tattccggat tgggaaagtc aatatctgga tttaatcgaa gaattatacc attacgttga
    12721 tcccgccagg attgcttgga taagtctcgg ttccttgcgt tttcccccgt caatgaaaac
    12781 taaaataatc gcgcgttacc cgtattcacg cctaccttat ggtgaactaa ttcgtggtca
    12841 agatcaaaag atgcgctatc ctcgtccgcg gcgaaccgaa atataccgtt ccatttaccg
    12901 caaactgacc gagattcgcg agccaccatt tgtttatttt tgtatggaga gtccgactgt
    12961 ctggcaagac gtctgcggct ttgcgccgca atcgaatgct catctggatt atttatttgc
    13021 ggaaagtct
//
LOCUS       DAOMJU010000053        12910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_4863_length_12910_cov_18.550836, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000053 DAOMJU010000000
VERSION     DAOMJU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4863_length_12910_cov_18.550836"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLO58_06240"
     CDS             complement(<1..201)
                     /locus_tag="PLO58_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74423.1"
                     /translation="MNLSAYRFRLAIPFLGLLFLSGWAQIPCADFEIVESIPIETILD
                     NPEIRNTSEVWAEMIGAADETID"
     gene            complement(215..874)
                     /gene="plsY"
                     /locus_tag="PLO58_06245"
     CDS             complement(215..874)
                     /gene="plsY"
                     /locus_tag="PLO58_06245"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQO74424.1"
                     /translation="MITLLAIIVISYLIGSIPTAIIVGKITKKIDIRQYGSCNAGATN
                     AFRVLGWKAGLFVALFDIAKGAFTTAYISQIRFDIPPLNPTWLMILAGLCAILGHTFT
                     IFAGFKGGKGVAVGAGMIIALYPLALLPCLLVFSIVLFSTGIVSVSSISAALSLPIIL
                     LLFNYFGTSPIDKALLIFSIFIPIFIVYTHRANIRRLFKGEEKPFEKLILLRRRRSSK
                     K"
     gene            complement(874..2658)
                     /locus_tag="PLO58_06250"
     CDS             complement(874..2658)
                     /locus_tag="PLO58_06250"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQO74425.1"
                     /translation="MKIKYIDGKRFIRGIKVGAYKIVNHQDYLNKINVFPVPDADTGT
                     NMAATMKTIVDNIQEDTNQIDEATKNIANSALEGARGNSGVILAQFFYGLAKGIGKNK
                     RLTTIQFGEAVNSAKSHAYEALSKPREGTILSVIKDWAENIYENSKNIFDFQELLNHS
                     LEVARHSLEKTRTKIEAMRNANVVDSGAQGFVYILEGISNFINRGRLKELEKNCIQPV
                     SVIARTEVLPEQITYRFCTECLIEGKNIPIENLRLEFEPYGDSIVLAGSPQKARLHIH
                     TNEPAKVFKIAHQYGDLLQQKADDMLKQYLIAHNPHPKIALVVDSACDLPPEFIDRSF
                     IHIIPIRLTFGQSSYLDKVTITPEYFYEMLDTEPEHPRTSQPSPADFINLYSFLLSHY
                     DSIISIHLPATLSGTYQNALNAAQNFPERKISVIDSKALSVSFGFLAEQAVELIAAGK
                     SHNEIVSELEKATRRTQLFVGVPSLKYLMRSGRVSKAKGLIANLFNIKPILNLDEYGS
                     PQHISKSFSKKGAFTKVLQMASNFAAQKVNPRFAIAHANNLKIAEYFNNELKKRFKVE
                     KINILPVAPMLGAHAGNGAAAIAVTWSE"
     gene            complement(2745..3425)
                     /locus_tag="PLO58_06255"
     CDS             complement(2745..3425)
                     /locus_tag="PLO58_06255"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HQO74426.1"
                     /translation="MPKDTANPKSNWWQKWKTSQRNKIIAGIIALIPVVLTIYFIKLV
                     ISFFDRIVSPVLDPLIGFHLPGLGLIVALIVIYLLGLFITNFIGKSLILLIEKWLNYI
                     PIVRTIYNTSKQVMNALSFSKAGFEKTVIVEYPRKNTWAIGFLTGEVSDHNGKRFLSI
                     FLPTTPNPTSGWVLLFPEDEVYSSDMNIEQGLKAIISAGAILPPKLNLRSTDKVNFSE
                     KNDSQIKQ"
     gene            complement(3452..3928)
                     /locus_tag="PLO58_06260"
     CDS             complement(3452..3928)
                     /locus_tag="PLO58_06260"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HQO74427.1"
                     /translation="MTNQSSHPNWIIGLFLILLGLLIFLIQFDYFDWSDFWPLIIIGV
                     GLLFLIGFFVNRNNYGLLMPAIILLIVGTLFLYLERTTWTNMNYLWPIFILAPGVGFF
                     FMYFFGPQGNNLWIPGTILIVISVIFLAGEWTILRYWPVILILLGIYLIFSKKPSS"
     gene            complement(4027..5076)
                     /locus_tag="PLO58_06265"
     CDS             complement(4027..5076)
                     /locus_tag="PLO58_06265"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HQO74428.1"
                     /translation="MSKIEEYLGKDADFLLNHKCTTISKELLHLPGPDFVDRILLYSD
                     RPVGVLRNLQTMYNTGRLSGTGYLSILPVDQGIEHSAGASFAPNPMYFDPENIVKLAL
                     EGGCNAVASTLGVLGSVARKYAHKIPFLLKLNHNELLSYPNMYDQTMFASIDQAFDMG
                     AVAVGATIYFGSAESRRQIMEVSEAFEYAHQLGMVTVLWCYLRNNAFKTAEKDYHVAA
                     DLTNQANHLGVTIEADIIKQKLPECNGGFLALKFGKTNKKVYEQLTTDHPIDLTRYQV
                     AGCYMGRAGLINSGGASGEDDLTEAVKTAVINKRAGGMGLILGRKAFQRPMAEGVKIV
                     NAVQDVYLDKNVTIA"
     assembly_gap    5094..5193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5239..5673
                     /locus_tag="PLO58_06270"
     CDS             5239..5673
                     /locus_tag="PLO58_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQO74429.1"
                     /translation="MSSKLINIKGELEKRDIKPTFQRLCVLELMEQSSEHLTADAIYE
                     SLLKKMPTMSRTTIYNTLNLFLEKGLIAPIMITGTEVRYEISTNQHHHFLCLQCNRIY
                     DVDVQCPLFESKEVDGHKIQEIHGYFKGICRNCLKQIEREPK"
     gene            5670..5894
                     /locus_tag="PLO58_06275"
     CDS             5670..5894
                     /locus_tag="PLO58_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQO74430.1"
                     /translation="MKEKVAEVIESIRPMLMADGGNVELVEVTKDGVVRLRLTGACGG
                     CPMSTYTLRMGIERRLKEAIPEIKSVEQVF"
     gene            5959..6162
                     /locus_tag="PLO58_06280"
     CDS             5959..6162
                     /locus_tag="PLO58_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HQO74431.1"
                     /translation="MKYTFKIPTMSCQHCKMRIEKALSPYVNPANIAFDLTSKKVAVE
                     TDIPLTILIEAIESVGYPVERDN"
     gene            6183..6758
                     /locus_tag="PLO58_06285"
     CDS             6183..6758
                     /locus_tag="PLO58_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQO74432.1"
                     /translation="MPSLKGTRTEKNLLTAFAGESQARNRYNYFASQAKKEGYEQIAA
                     VFAETADNEKEHAKRFFKFLEGGEISITAAFPAGVIGTTAENLLEAANGEKYENSVMY
                     PEFAKVADEEGFTEIANVFRAVIVAETQHEKNYRALRKNVLENRVFEKEQVVTWRCRN
                     CGYIHVGTKAPEKCPACAHPRAYFELLAENY"
     gene            6796..6960
                     /locus_tag="PLO58_06290"
     CDS             6796..6960
                     /locus_tag="PLO58_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQO74433.1"
                     /translation="MTKYVCTICGYVYDPRKGDPDNGVPRGTAFEDLPDDWVCPQCGA
                     EKEDFEPQED"
     gene            6963..8144
                     /locus_tag="PLO58_06295"
     CDS             6963..8144
                     /locus_tag="PLO58_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HQO74434.1"
                     /translation="MAVQILKPDIFSVGARDWDRRLFDELIPLPDGTTYNAYLIKGSQ
                     KTALLDTVDPPKKHELLENLQEIGIERIDYLISHHAEQDHSGTIPAVLEQFPMAQVVT
                     NQKCRALLQEHLLIPDEKFKIINDGEELSLGNKTLRFIFTPWVHWPETISTYLEEDKI
                     LFSCDFFGSHLATSNLFVDDESRVYRSAKRYYAEIMMPFRSNVRQNLEKVKNLAIEMI
                     APSHGPIYPNPEFIMEAYADWTSDSVKNIVLIPYVSMHGSTEAMVQYLTKALIEREIT
                     VQPFNLSRTDIGELAMAVVDAATVIIGSPTVILGPHPAAVSAAYLVNLLRPKTRYLTI
                     IGSFGWGGKMPEQIKALLSGMKAEFLSPVIIKGYPKLEDFSALDRLADEIAARHRALS
                     S"
     gene            8156..8632
                     /locus_tag="PLO58_06300"
     CDS             8156..8632
                     /locus_tag="PLO58_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQO74435.1"
                     /translation="MTIFNIRDVFDIAVKIEERGEKFYRETAKVISKAEVKELFEKLA
                     DEEVAHKKIFRQMAQKIGAVKLESAAREEFYDYLEAYTQNLIFSDANDETKIPAINDA
                     KTALLYAIERELDSVLYYKEIKELIPASEHQLISGIIDEERRHVVRLSELKKNLTF"
     gene            8752..9843
                     /locus_tag="PLO58_06305"
     CDS             8752..9843
                     /locus_tag="PLO58_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQO74436.1"
                     /translation="MKFKNEHPRRVLTARQRKRPEDIRGPFFRDQTAIIHSLAFRRLK
                     HKTQVFFSPENDHICTRIEHSLHVATISATICRALKLDIDLATAIGLGHDLGHAPFGH
                     AGEKWLENLFGGFVHELHSLRVVDKLENYGQGLNLTYAVRDGIVSHCGEVDEQFIEIS
                     PTLNDLDNLQFRPVQPSTWEACVVRLSDSIAYLGRDLEDAVAANLINKEDLPEKLRRG
                     IGQSNSEIINNLVFDVIDWSTKNGKIGFSPEKFALISEFKEFSREKIYDNPKLKYWRS
                     YSGFVIETLKNYLIDLYEKYQNDYERYANSRTPLDVKFGKYLKSLHERYQAEKCSSEL
                     IVRDYIAGMTDAYALHCFQNILTMGTRGL"
     gene            10129..11334
                     /locus_tag="PLO58_06310"
     CDS             10129..11334
                     /locus_tag="PLO58_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HQO74437.1"
                     /translation="MKKNGWLVIFLLCVPLMAQKFDKMALTPPMGWNSWNRFGCDVNE
                     QLIRETADAMVSSGMKAAGYQYVVIDDCWQGERDALGFIQPDPGRFPSGMKALADYIH
                     SKGLKFGIYSDAGWKTCGGHPGSRGYEYQDALTYAKWGVDYLKYDWCNTEGLNAEGAY
                     LTMRDALFAAGRPIVFSICEWGDNQPWEWGKDIGHLWRTTGDITAIFDGQEGHGTWNS
                     WGVSQILDMRENIRQYAGPDHWNDPDMLEVGNGMTIAEDRAHFSLWCIQAAPLIAGND
                     LRDMSSEVIDILTNKELIAIDQDPLGIQGFKYLVKDNWEVWAKPLDKGDLALCFLNRT
                     LEPVKVDLDWKKYPVKDDEFHYYYLFNENEYKIRDLWLKKEIGTTAKPFSTQIGAHDV
                     IVLRLSPVK"
     gene            complement(11337..12044)
                     /locus_tag="PLO58_06315"
     CDS             complement(11337..12044)
                     /locus_tag="PLO58_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74438.1"
                     /translation="MNNNTEIIDLKKEQISSLERLHYKYSLMARAFFVLMDIVTGYQI
                     TLSKAKLIEILASIPYRAWEVRHYSRLTRVYRNYEKVTRSQRIVTWSRSAQDNEYWHL
                     LVIHEKMKTDGIRDAWYLFPLVPYVMVAVYRLFAKTLAFCNIRRAYLFNAEFEDHAEH
                     VYAQFVADHPEWETQPVESDLVNEYGKFSSWADVFRRIGLDERDHRNHSFLLCGKPEA
                     VVSYNGMPKDINFPTGR"
     gene            complement(12218..12559)
                     /locus_tag="PLO58_06320"
     CDS             complement(12218..12559)
                     /locus_tag="PLO58_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQO74439.1"
                     /translation="MNIYVGNLAPNVTEGDLRVAFEAFGQVASASVIKDKFTGESRGF
                     GFVEMPLMAEAQAAISNLNGKDLKGKTITVNEARPRTEGGRGGDRFGGHSGSGHFGGK
                     PRDNRRGGGRY"
BASE COUNT         3826 a         2484 c         2854 g         3646 t
ORIGIN      
        1 gtcaatagtc tcatcagccg cgccgatcat ttctgcccag acttcggaag tgttacgaat
       61 ttcgggatta tcgagaatag tctcaatcgg aatgctttcg acaatttcaa agtcggcaca
      121 cgggatttga gcccaacccg agaggaataa cagccccaaa aacggaattg cgagacggaa
      181 ccgataggct gacaaattca tcctaaacaa aaagttactt tttagaagat cgccgccgac
      241 gtaggagaat taatttttcg aatggctttt cttcaccttt aaaaagtcgc cgtatattag
      301 cacgatgagt ataaacaata aaaattggga taaaaatact gaaaattaga agtgctttat
      361 ctatcggcga agtcccgaaa taattgaaaa gcagtaaaat gatcggcaaa ctgagggcgg
      421 cgctgattga cgagactgat acgataccgg tcgagaatag gacaatgcta aagaccagca
      481 aacatggtaa aagcgcaaga ggatataaag cgataatcat cccggctccg accgcaacac
      541 ccttgccacc tttaaaaccg gcaaagatgg tgaaagtatg gccaagaatc gcacataaac
      601 cggctaaaat cattaaccat gtgggattta acggtggaat atcgaagcgg atttgggaaa
      661 tgtaggcggt agtaaatgcc cctttggcaa tgtcaaaaag agccacgaaa agtccggctt
      721 tccagcctag aacgcgaaac gcattcgtgg cgccggcatt gcacgaaccg tattggcgaa
      781 tatcaatttt tttagtgatt ttcccgacga tgatggcggt gggaatcgaa ccgattaagt
      841 agctgattac gattattgcc agtaatgtta tcatcattca ctccaggtaa cagcgattgc
      901 cgccgcaccg tttccggcat gcgcgcctaa catcggcgca acaggtaaga tattgatttt
      961 ttcaaccttg aaacgttttt ttaattcatt attgaagtat tcagcaatct tcagattgtt
     1021 ggcgtgagcg atagcaaacc gtggattgac tttttgagct gcaaaattgc tagccatctg
     1081 cagaacttta gtgaaagccc cctttttgga aaaacttttc gatatgtgtt gaggtgatcc
     1141 gtattcgtcc aaatttaaga tcggtttgat attgaataag ttggctatca agcctttagc
     1201 cttcgagact cgcccgctac gcattagata tttcaaactt ggtactccga caaataattg
     1261 tgtccgacga gtagcttttt ccaattcact gacgatttcg ttatgagatt taccagcggc
     1321 aattaattcc accgcctgtt cggcaagaaa accaaaagaa accgaaagag ccttactatc
     1381 aatcacgcta attttccgtt cgggaaaatt ttgggcggcg tttagggcat tctggtaagt
     1441 accactaaga gtggctggca ggtgaattga aataatcgaa tcatagtggc tgagcaagaa
     1501 actatataaa ttgataaaat cagccggtga cggctgagaa gtacggggat gttccggctc
     1561 cgtgtcgagc atttcataaa aatattcggg agtgattgtg actttatcaa ggtaactcga
     1621 ttgaccaaac gtcaatcgta tcggtataat atgaataaag gagcggtcga tgaattcagg
     1681 tggcaaatcg caggctgaat ctactaccaa cgcaattttc ggatgtggat tatgggcaat
     1741 taaatactgc ttaagcatat cgtcggcttt ttgctgaagg agatcgccat attgatgggc
     1801 aattttaaaa actttagccg gttcgttagt atgaatatgc aaacgtgcct tctgcggtga
     1861 accggctaag acaatcgaat cgccgtatgg ttcgaactcc agacgtaggt tttctatcgg
     1921 aatattttta ccttcaataa ggcattcggt acaaaaacgg taggtaatct gttccggtag
     1981 tacttccgtg cgggcgatga ctgagaccgg ctgaatacaa tttttttcca gctccttgag
     2041 tcttccacga ttgataaaat tactgattcc ttccagaata taaacgaatc cctgtgcgcc
     2101 ggagtcaacg acgttggcat ttcgcatcgc ctcaattttg gtgcgggttt tttccaaaga
     2161 atgtcgggca acttctaggg aatgatttag taactcctga aaatcgaaaa tattcttgct
     2221 gttttcgtaa atattttcag cccaatcttt gatgacagat aggatggtac cttctcgcgg
     2281 tttggatagc gcttcgtaag catgactttt ggcagaattg acagcctcgc caaattgaat
     2341 ggttgtaaga cgtttatttt ttccgattcc ctttgccaat ccatagaaaa actgagccag
     2401 aataacgccg ctattgccgc gcgctccttc gagtgctgaa ttggcgatgt ttttcgtcgc
     2461 ttcatcaatc tggtttgtgt cttcttggat attatccact atggttttca tagtggcggc
     2521 catattggtg cctgtatctg cgtcaggaac aggaaagaca ttgattttgt tgagataatc
     2581 ctgatggtta acgattttat acgcaccgac tttgatgcca cgtataaatc tcttaccatc
     2641 aatgtattta attttcataa atgatggtct cttctctatt ttttatcatt ttataactat
     2701 taatgttgtc ctcaaaattt atattcaagt taattaaaag ttttctattg tttgatttgg
     2761 gaatcatttt tctcggaaaa gtttacttta tcggttgatc taagatttaa ttttggtggc
     2821 aagatagctc cggctgatat tatagcctta agcccttgtt caatattcat atcggaagag
     2881 taaacttcat cttcgggaaa gagcagaacc cagccggaag ttggattggg agtcgtcggt
     2941 agaaaaatac tcagaaatcg tttgccatta tggtcgctaa cttcacctgt taagaatcca
     3001 attgcccagg tatttttacg gggatattcg acgataacgg tcttttcaaa accggctttg
     3061 gaaaaggaaa gagcattcat tacttgtttg ctcgtattat aaatcgtgcg aacgatggga
     3121 atgtaattaa gccatttctc aattaggaga attaatgatt ttccaatgaa attagtaata
     3181 aaaagaccaa gcaaatagat gactataaga gccacgatta aacccaaccc gggcaagtgg
     3241 aaaccaatca gaggatcaag taccgggcta acgatccgat cgaaaaaaga aataactagc
     3301 ttaatgaaat aaattgtcaa cacaaccgga attaaggcaa ttattccggc aattatttta
     3361 ttgcgttgag aagttttcca cttttgccac caattggact taggattggc tgtgtctttt
     3421 ggcatttggg aattttcggg agaaggtggg ttcatgaaga aggtttttta ctaaaaatta
     3481 gataaatacc aagtagaatt aatataactg gccaatagcg taaaatagtc cattcaccag
     3541 ccagaaaaat aacggaaatc acgattaata tagttccggg aatccagaga ttattgcctt
     3601 gtggtccgaa aaaatacata aagaaaaaac caactcccgg tgctaatata aaaatcggcc
     3661 agaggtaatt catattagtc caagtggttc tttccaaata aagaaacaaa gtcccgacaa
     3721 ttaaaagaat gatagcgggc atcaatagcc cataattatt gcggttgacg aaaaaaccta
     3781 ttagaaataa taatcctact ccgataatga tcagcggcca aaaatcagac cagtcaaagt
     3841 aatcgaactg aattaagaat atcagcaaac ccagcagaat taaaaatagt ccgattatcc
     3901 aattgggatg cgaagattga ttagtcattt cctacctcga aaaaggtaaa gccccgaacg
     3961 gggctttacc atcttgattt tattatccaa ggatagcctc ggtatggatt gcccaattga
     4021 ttcaggttag gcaatcgtaa catttttgtc gagataaaca tcctggacgg catttacgat
     4081 cttgacgcct tcagccattg gtctctgaaa agctttgcgt cccagaatca aacccatacc
     4141 gcccgcccgt ttattgataa cggctgtttt gacagcttca gttaaatcgt cttcaccgct
     4201 ggcgccaccg gaattaatca atccagctcg tcccatatag caaccggcga cctgatagcg
     4261 agtaaggtca atcggatgat cagtagtcaa ttgttcgtaa actttcttat tagtttttcc
     4321 gaactttaag gctaagaatc caccattgca ttcgggtaat ttttgcttaa taatatcggc
     4381 ttcaatggtc acaccgagat gattagcctg attggttaaa tcagccgcga catgatagtc
     4441 tttttcagca gtcttaaagg cattgttacg taagtaacac catagtaccg taaccatacc
     4501 aagctgatgc gcatattcga atgcttcact gacttccata atctgacgac gtgattccgc
     4561 cgaaccaaaa taaatcgtag cacccacggc aactgcgccc atatcaaaag cttgatcaat
     4621 actggcaaac atcgtctgat catacatatt aggataggat agcagttcgt tgtgattaag
     4681 tttcagcaga aagggaattt tgtgggcgta tttacgggcg acgctcccca gaacgccgag
     4741 cgtcgaagct acggcattac atccgccttc gagggctaat ttgacaatat tttccggatc
     4801 gaaatacatc ggattcgggg caaatgaagc tccggcggaa tgctcgatgc cctggtcaac
     4861 cggcagaatc gagagataac cagtacctga caaacgaccg gtgttataca ttgtttgcaa
     4921 attgcgcagg acgcctactg gccgatccga ataaagcaaa atccggtcaa caaaatcagg
     4981 cccaggcaaa tgcaatagtt ccttggaaat tgtcgtgcac ttatgattca gtagaaaatc
     5041 cgcatctttt ccaagatatt cttcaatttt actcatacaa aaacctccaa aaannnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatattac ttgaattttt ccataactgt
     5221 tgttaatatt aacttgtaat gagttcaaag ttgataaata ttaaaggtga attggaaaag
     5281 cgcgatatta aaccgacttt ccagcgctta tgtgttttag aattaatgga acaaagtagt
     5341 gagcatttga ctgccgatgc gatatatgaa agtctgttaa aaaagatgcc aacgatgtcg
     5401 cggacgacga tctataatac cttgaattta ttcctggaaa aaggactaat agcgccgatt
     5461 atgattaccg gcaccgaagt ccgctatgaa atctcaacca atcaacacca tcattttctc
     5521 tgcttacagt gcaaccgaat ttatgatgtt gacgttcaat gtccgttatt tgaaagtaag
     5581 gaagttgatg ggcataaaat ccaggaaatt catggctatt tcaaaggaat atgtcgaaac
     5641 tgtctaaagc aaatagaaag ggaacctaaa tgaaagagaa agttgcagaa gtaatcgaat
     5701 ccatccggcc gatgttgatg gctgatggcg gtaatgtcga attagtcgag gttaccaaag
     5761 atggagtcgt aagattaaga ttaacaggtg cttgcggcgg ttgtccaatg agcacttata
     5821 ctttgcggat gggcatcgag cgccgtctca aagaagctat tccagaaatc aagagcgttg
     5881 agcaggtttt ttagcagaaa ataatctgat tttaaaatca cgattctatg attaatttta
     5941 tcctagaaaa aatgatttat gaaatatacg ttcaaaattc cgacgatgag ctgtcagcat
     6001 tgtaaaatgc ggatcgagaa agccttgagt ccgtatgtca atccggctaa tatcgctttt
     6061 gatttaactt caaaaaaggt agcagtggag actgacattc ccttgacgat attgattgaa
     6121 gctatcgaat cggttggtta tcccgttgaa cgtgataatt aataataaga aaggagaaat
     6181 atatgccaag tttaaaagga acccgcacgg aaaaaaactt gttgaccgct tttgccggtg
     6241 aatctcaagc tcgtaatcgt tataattatt tcgcttctca agccaaaaag gaaggctatg
     6301 aacaaattgc cgcggtattt gcagaaacgg ctgataatga gaaggaacat gccaagcgtt
     6361 tcttcaaatt tttagaaggc ggcgagatta gcataaccgc cgcatttcca gccggagtaa
     6421 ttggcacgac agccgagaat ttgctggaag ccgccaatgg tgaaaaatat gaaaattcgg
     6481 taatgtatcc tgaatttgct aaagtcgctg atgaggaagg gtttaccgaa attgccaatg
     6541 tttttcgagc cgttattgtt gctgaaaccc agcacgagaa aaattatcgt gctctgcgta
     6601 aaaatgtgct ggaaaaccgc gtttttgaaa aggagcaggt tgtgacatgg cgttgtcgaa
     6661 actgcggtta tattcatgtg ggtactaagg caccggagaa atgtccagcc tgcgcccacc
     6721 ctcgagctta ctttgaacta ttggctgaaa attattgatt aaataggatt tattaactca
     6781 tttacaggag gtaatatgac taaatatgtt tgtactattt gtggttatgt ttatgatcct
     6841 cgcaaagggg atccggataa tggtgtgccc cggggcactg cctttgagga tttaccggat
     6901 gactgggttt gtccgcagtg tggcgctgaa aaagaggatt ttgaaccaca ggaggactga
     6961 aattggcagt ccagatttta aagccggata tttttagcgt tggtgcccgc gattgggatc
     7021 gccggttgtt tgatgagttg atccctcttc cggacggcac gacttataat gcttatctga
     7081 ttaaaggttc gcaaaagacg gctttgctcg ataccgtcga tccgcctaaa aaacatgaat
     7141 tgctcgaaaa tctacaggag attggaatcg aacgcattga ttatctaatt tcgcatcatg
     7201 cggaacaaga tcattctgga actattccgg cggttttgga acaattccca atggcgcagg
     7261 tagttactaa tcaaaaatgt cgggctctac ttcaagagca tttgttaatt ccagacgaaa
     7321 aattcaaaat tattaacgac ggtgaagaac tcagcttagg gaataaaact ttacgtttta
     7381 tttttactcc ctgggttcac tggccggaaa ccatttcgac ctatctggaa gaagataaaa
     7441 tcctgttctc ctgtgatttt tttggttcgc acttagcaac gagtaattta tttgttgatg
     7501 atgaaagtcg agtttatcgc tccgctaaac gctattatgc cgaaattatg atgccgtttc
     7561 gctcgaatgt gcgtcaaaat ctggaaaaag taaagaatct tgccattgaa atgattgctc
     7621 ccagccacgg accgatttac ccaaatccgg aatttattat ggaggcttat gccgattgga
     7681 cttcggattc ggttaaaaat atagtgctta taccgtatgt ctcaatgcat ggcagtaccg
     7741 aagcgatggt tcagtatctc acgaaggctc tgattgagcg tgagattacg gttcagcctt
     7801 ttaacctgtc cagaaccgat ataggggaat tggcaatggc ggtggtggat gcggctacgg
     7861 taataatcgg atcaccaact gtgattctcg gtccgcatcc agcggcagtc agtgcggctt
     7921 atttagtaaa ccttttacga cctaagaccc ggtacttgac gattatcggc tcttttggct
     7981 ggggtggaaa aatgcccgag cagatcaaag ccttgctaag tggaatgaaa gccgagtttc
     8041 tgtctccggt aattattaaa ggttatccca aattggagga cttttctgcc ctagatagat
     8101 tagcggatga aattgccgcc agacaccgtg ctttaagttc ttaaggagat aattgatgac
     8161 tattttcaac atccgtgatg tatttgacat cgcggttaaa attgaggaaa gaggcgagaa
     8221 attttaccgc gaaacggcaa aagtcatttc gaaagcagaa gtcaaggaac ttttcgaaaa
     8281 actggctgat gaagaggtcg cgcataagaa aatattccgg cagatggcgc agaaaatcgg
     8341 agcagtaaag ctggaatcgg cggctcgcga ggaattttac gattatctgg aagcctatac
     8401 ccagaacctg attttttctg atgccaatga tgaaaccaaa ataccagcaa ttaatgatgc
     8461 taaaacggca ttgctttatg ccattgaaag agaactcgat tcggttctgt attataaaga
     8521 aatcaaggag ttaattccag ccagtgagca tcaattgatt agcggaatca ttgacgaaga
     8581 gcgccgccat gtcgtccgac tttcggaatt gaagaagaat ctgacgtttt aatcaaagag
     8641 attcttaccc gataatttgg cttaggtaat gagcgtgaaa aacgctgtat tttgaatttt
     8701 tcatcgtaat atattcagga ataaaaatga ctatagagga taaaatcgca attgaaattc
     8761 aagaatgaac atccacggcg agttttaacg gcgcgccagc gtaagcgacc ggaagatatt
     8821 cgcgggccat tttttcgcga tcaaacggct attatccatt cactggcatt tcgaagacta
     8881 aaacacaaaa cgcaggtctt tttttccccg gaaaatgacc atatttgtac ccggattgaa
     8941 cactctttac acgtggcgac gatttcagcg acgatttgcc gagctttaaa attggacatt
     9001 gatttagcaa cagcgatcgg gttgggacac gatttggggc acgcgccttt tggccatgct
     9061 ggtgaaaaat ggctggagaa tttatttggc ggcttcgtgc atgaattaca cagtctgcga
     9121 gtggttgata aactcgaaaa ttatgggcaa gggctgaatc tgacctatgc ggtgcgtgat
     9181 ggaatcgttt cgcattgtgg cgaagttgac gagcaattta tcgaaatcag tccaacactt
     9241 aacgatctgg acaatttaca atttcgccca gttcagcctt ccacctggga agcctgtgtt
     9301 gtacgcctca gcgattcaat cgcctatttg ggacgtgatc tggaagatgc agtcgcggct
     9361 aatcttatta acaaggaaga tttgccggag aaattgagaa gaggtatcgg acaatccaac
     9421 agtgaaatta tcaataatct ggttttcgat gtcattgatt ggtcaacgaa aaatggcaaa
     9481 attggttttt caccggaaaa atttgccctg attagcgaat ttaaagaatt tagtcgggaa
     9541 aaaatctacg ataatccaaa gctaaaatat tggcggagtt actccggctt tgtaatcgaa
     9601 accctcaaaa attaccttat tgatctgtac gaaaaatatc aaaatgacta tgagagatat
     9661 gctaatagta gaacaccact ggatgtaaaa tttggcaaat atctcaaatc cttgcatgaa
     9721 cgctatcagg ctgaaaagtg ttcttcggaa ttgatcgtgc gtgattatat agctgggatg
     9781 acagatgctt acgccttaca ttgctttcag aatatcttaa cgatggggac ccgcggactt
     9841 tgacatcagt gacaaccgat aactaatggg ttagaagcct cttattatat tactgagttt
     9901 agtacttttt tatttttaac ctttgcaatt ggtgcagatt agcccaataa tttttcagtt
     9961 gagtcattta taaaaatttg gtataagaaa ttttcacctc ttgctttgca attcgatttt
    10021 catctaaaaa actcttgaaa ttcactttaa tttaacttac aatccgaagt ggattttatt
    10081 ataaaaaacg taagctttct gaaaacaaaa taaatggggg atacttttat gaagaaaaat
    10141 ggttggttgg tgattttttt gttgtgcgtt ccattaatgg ctcaaaagtt cgacaaaatg
    10201 gcgctgacgc caccgatggg ttggaatagt tggaacaggt ttgggtgcga tgtgaatgaa
    10261 caactcattc gggaaacagc cgatgcgatg gtgtccagtg gtatgaaagc cgctggttat
    10321 cagtatgtgg tgattgatga ttgttggcaa ggggaacgcg atgctctggg atttattcaa
    10381 cctgatccag gaaggtttcc atctggtatg aaagctctgg ctgattatat tcattccaag
    10441 ggattgaaat ttgggattta ttcggatgcc ggttggaaaa catgtggtgg tcatcccggt
    10501 agccgtgggt atgagtatca ggatgcactt acttatgcca aatggggagt tgattatttg
    10561 aaatatgatt ggtgcaatac cgaagggctt aatgctgaag gggcttatct gactatgcga
    10621 gatgctttgt tcgcggctgg tcggccaatc gttttcagca tttgtgaatg gggcgataat
    10681 caaccctggg aatggggcaa agatattggt catctctggc gtacgaccgg cgacataact
    10741 gccatttttg atggacagga ggggcatggt acctggaatt cctggggtgt gtcacaaatt
    10801 ctcgatatgc gcgaaaatat ccgtcagtat gccggaccgg atcattggaa cgatccggat
    10861 atgctggaag tcggcaatgg gatgacaatc gccgaagatc gggcacattt ttccctgtgg
    10921 tgtattcagg cggcaccttt aattgcgggc aacgacctca gagatatgag ttcggaggtg
    10981 atagatattt tgactaataa agagttgatt gccattgatc aggatccatt gggaatccag
    11041 ggttttaaat accttgttaa agataattgg gaagtctggg caaaaccgct ggataaaggc
    11101 gatctggcac tctgcttttt aaatcgaacg ttagaaccgg tgaaagttga cctcgattgg
    11161 aaaaaatatc cggtcaaaga tgacgaattt cattactatt atctttttaa tgaaaatgaa
    11221 tacaaaattc gtgacctttg gttgaaaaaa gagattggga caactgccaa accgttttcg
    11281 actcaaatag gtgctcacga tgttattgta ttaaggctaa gtccggtcaa gtaaaactac
    11341 cgaccggtcg ggaaatttat atctttgggc attccattat aactgacaac tgcttccggt
    11401 ttaccgcaga gtagaaaact gtgattgcgg tgatcacgtt cgtcgagtcc gattcgtcgg
    11461 aagacatctg cccaggagct aaattttccg tattcattga ccaggtcgct ttcgaccggc
    11521 tgagtttccc attccgggtg atcggcaacg aattgggcat agacatgttc ggcatgatcc
    11581 tcgaattcag cattgaagag ataagcgcgc cggatattgc agaaagccaa agttttggca
    11641 aaaaggcgat atactgcaac cattacatac ggaacgagcg gaaaaaggta ccaagcatct
    11701 cgaataccat cggttttcat tttttcgtga atgaccagta aatgccagta ttcattatcc
    11761 tgggctgatc ggctccaggt gacaatccgt tgacttcgag tgactttttc ataattgcgg
    11821 taaacgcggg tcagacgaga ataatgacgt acttcccagg cgcgatacgg gatactggct
    11881 aagatttcga ttaatttagc tttagataag gtaatctggt acccagtaac aatatccatc
    11941 agtacaaaga aagcacgtgc catcaacgag tatttataat gaagccgttc aagagaactg
    12001 atctgttctt ttttaagatc aataatctcg gtattattat tcatgcttta tccagcgatt
    12061 ttaatcaaca atcaggaata taattaaaat cggtagttca aacaataaac cagtggaatt
    12121 tgcattgcaa ttccatatat aactggctat cctctattta agtagtggat agccagttat
    12181 tattaatttg gcttggattt agccgacgta cagcagacta atagcgtccg ccgccgcgcc
    12241 gattgtcccg cggtttgcca ccgaagtgac cgctaccaga atggccgccg aagcgatcgc
    12301 caccgcgacc gccctctgta cgaggacgag cttcattgac ggtaatggtt ttgcccttca
    12361 agtctttgcc gtttaaattg ctgatagcgg cttgagcttc agccattaaa ggcatttcga
    12421 caaagccgaa gcctcttgat tcgccggtga atttatcttt aataacagat gcactggcga
    12481 cctgtccgaa agcctcgaaa gcaacgcgta agtcaccttc agtgacattc ggtgctaaat
    12541 ttcctacgta gatgttcatt atgacctcct tttgatcttg aacgctttaa cgtactttct
    12601 tttacattga ccaaatgctt atctctaagc gggttccata acccaactcc agaaagtagt
    12661 ttccgccagt acccgcaaaa tgggtatttt cgtgatcaaa aaaggtctac cactaagtga
    12721 aaaagttttt ctggttggtt tgtgattgat ggttactcgt ttttcgaaaa gcgttgtatt
    12781 aaaatacttt aaaccctgca gttaaacaag ctttattttt tagagtgtta attgtagtag
    12841 tttttgtgaa taaaattttc tatgtcttac tggattaata aatagaacat attacttaat
    12901 aattaataac
//
LOCUS       DAOMJU010000054        12770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_4931_length_12770_cov_20.056705, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000054 DAOMJU010000000
VERSION     DAOMJU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_12770_cov_20.056705"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1191)
                     /locus_tag="PLO58_06325"
     CDS             complement(157..1191)
                     /locus_tag="PLO58_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQO74440.1"
                     /translation="MPKSIATYILPLAIVIISALIGLLLDRLVFSKWRKNVQKIRPPV
                     LRVVGNSMRGILIIVMGLVGLYFALSYFGIEEEVLNNLRKLLIVLIILTFTILLARIA
                     VGFIENLGRRKKMPSLTLFINLTKIVIYVIGFLIILQTLGVSITPLLTALGVGGLAVA
                     LALQDTLSNLFAGIFIIGTRLVQPDDYIMLSSGVEGFVKDINWRNTIIRTMANNENVI
                     PNSELSKTIITNYSRPDKKMSLIVPVLVGYNNDLNKVEEVLIQTAREVLNEVEGGVKD
                     SEPIVRFNRFNESSVSLNLIVKVRKYEVQFPVQHELIKRIHRRFKEENIEIPYPVRTV
                     YLKPGSENEI"
     gene            1494..1967
                     /locus_tag="PLO58_06330"
     CDS             1494..1967
                     /locus_tag="PLO58_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HQO74441.1"
                     /translation="MNTKRDRHRAIRQIIASRQISNQNELKDVLEKQGFVTTQATLSR
                     DLMTLKIIKVPDAERGYIYALSENFQSSKPPIEGSSPLQTCRSIAFSGNLAVLKCLPS
                     FAPSVALLLDAYEMNEIIGTIAGDDTVLIILKEGLSHERFRSALVERCPELRNRI"
     gene            2044..3294
                     /locus_tag="PLO58_06335"
     CDS             2044..3294
                     /locus_tag="PLO58_06335"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQO74442.1"
                     /translation="MSKKVILAYSGGLDTSVILKWLVNKGYDVICYVANVGQREDFAA
                     VEQKAFKTGASKVYIEDLRREFVNDYIFAALRGNAIYEGRYLLGTSLARPLIAQRQVE
                     IAQMEKTHYYAHGATGKGNDQVRFEFAYYALDTEAIVISPWKDPEFLAEFKGRSDLIR
                     YAEKWNIPVKASLTKSYSEDENLMHISHEAGVLEDPSFRPPENVFSWTVSPQDAPDSE
                     TILEIEFKDGYPIKVTNLNNGVVKEDSLELFYYLNEIGSQNGVGRVDMVENRFVGIKS
                     RGVYETPGATILWTAHRDLEGIAMDKEVMHLRDMLSPKFAELIYNGFWFSPEMDFLMN
                     AFEKSQEKIDGSVTVSIYKGNVTPIGRRSPISLYDQALSSMDIAGGFNANDSLGFIRV
                     NALRLKAHKIVLKKKKPYNWRIKD"
     gene            3298..4497
                     /gene="argH"
                     /locus_tag="PLO58_06340"
     CDS             3298..4497
                     /gene="argH"
                     /locus_tag="PLO58_06340"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQO74443.1"
                     /translation="MKLWQKKYDLNAAVEKFTVGNDYLLDQELVPFDCQASRAHARML
                     NKIGILSDSETADLLSGIDQILDLHAKGKFKIRPEDEDCHTAIENFLTEKIGIAGEKI
                     HTGRSRNDQVLTALRLYCKTQLNEISSAVQTTIATITEFIQKYGEVKMPGFTHTRKAM
                     PSSVAMWAGALRDALADDLDLLACATKLIDQNPLGTGAGFGVPLALDRQMTTDELHFA
                     RIQENPIYAQNSRGKFEGFALSVMSQIMYDLNKVASDLILFTMPEFNYFTLPDEFLTG
                     SSIMPHKKNPDVLELLRAKYHEVFAYEMQVRNTGVNLISGYHRDLQLTKEAIIRAFKT
                     TLTSVQITTVLFENLSVNRKNCRKAMTAELFATDKVYQLVKSGVPFREAYRQISQEYE
                     DKNEKQC"
     assembly_gap    4603..4702
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4703..5902
                     /gene="argJ"
                     /locus_tag="PLO58_06345"
     CDS             <4703..5902
                     /gene="argJ"
                     /locus_tag="PLO58_06345"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HQO74444.1"
                     /translation="YNNPKITDPKGFTGAAMHAGFKRKRNDLCIIKSTVPAKCSAKFT
                     TNAVKAAPLIVAMENLPKSGNRLQALIINSGNANAGTGQQGLNDVHETLNLLSAQLDI
                     PEELILASSTGVIGHCLDMEKMRNGITHLVPHLSIEGGAACAEAILTTDTVEKSFGVK
                     FRVNGAEIKLCGITKGSGMIQPNMATTLAFVTTDADIDHNFLEKTFTTTVAKTYNMIT
                     VDGHTSTNDTALIMANGLAGNQTITAGSPAGEIFENALLMLMTEMAKSIVRDGEGATK
                     FVTVTVNGADSFEDAKKMAFGIANSMLVKAALFGCDPNWGRILSAAGMVFAHFDPAVA
                     DLRINGIYLYRQGEPLNLNKTEIQRMMTPVELEIVLDCNLGKDSAVVYTTDLSYDYIK
                     INAQYHT"
     gene            5911..6804
                     /gene="argB"
                     /locus_tag="PLO58_06350"
     CDS             5911..6804
                     /gene="argB"
                     /locus_tag="PLO58_06350"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQO74445.1"
                     /translation="MQKIIDKAAVLIEALPFIKAFHGKKIVIKFGGSIMDSENAMKSV
                     LQDIVFMKYVGWLPVVIHGGGKAISEAMNNRGVKPQFVNGLRVTDRKTMEIVENVLAH
                     KINAQIVNLLNMMDGEAVGVSGLDNGLIRVDKMFTENNGQSVDLGFVGQVRYINANFL
                     NRLIESGKIPVIAPLGLGDDGESYNINADLVAGEIAASIGARKLVYLTDTPGILRTPG
                     DEKSIISTINRQEAEKLIAAGIIEGGMIPKVNSCLDALRFGVNKGHIIDGRIKHSLLL
                     EIFTKEGIGTEILKDGSERIR"
     gene            6782..7945
                     /locus_tag="PLO58_06355"
     CDS             6782..7945
                     /locus_tag="PLO58_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQO74446.1"
                     /translation="MDRKEFDNYVLPTYKRFPITLVKGRGMQVWDDEGNEYLDFFPGW
                     GCGNIGHCHPKVVAALHEQLDKIMHVPNVYYNEKAGELAKLIIQDSFDGQVFFGNSGA
                     EANEGALKLVRKRNPNKKTIITLNNSFHGRTLATVTATGQSEYQKGFDPLLGGFVYCD
                     INDYAMLASLMNENVAAIMLEVVQGEGGITPVTPKFIQTARELCDRFDAMLIIDEVQT
                     GIGRTGKMFAYQYFDVQPDVMTLAKSLGGGMPIGAFAVARQYCNILQPGSHGSTFGGN
                     PLACAAGVAVFQAIHEENLLENAVEMGTYFRQKLEELKSQAPVIREIRGLGLMLGVEL
                     NKPGAEIVDKCRAQNLLINCTHQTVLRLMPAINVTKEQIDKAVAIIGKALMES"
     gene            8123..10591
                     /gene="thrA"
                     /locus_tag="PLO58_06360"
     CDS             8123..10591
                     /gene="thrA"
                     /locus_tag="PLO58_06360"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HQO74447.1"
                     /translation="MKVLKFGGSSIGKPENLPTIFNIIAREVQSEKAVITVFSAFNNV
                     TDQLKEISQLAASKDKTYQNILDDLARKHFNFIEKLIPQDKQKPLNAEIKNLLAELKE
                     IIHGVFLLKELSLQSLDLILSFGERLACTIIAAYGQSIGYPTKYVDARQFLVTDDHFG
                     AARILPHISNPKIQAFFKNFTGIPVVTGFIGATADGITTTLGRGGSDLTASQIGAALN
                     AEEIQIWKDVNGFMSADPSKVKEAFSLAELSYEEAVELSHFGAKVLFPPTIQPVIDKN
                     IPIRIMNTFNPDFPGTVIRKNPSANGGYVKGVTSISDISLLTVQGSGMVGVSGISSRL
                     FSALAQVNVNVILISQASSEHSICVAILRESVNIAKKAIEEEFALEIKLRLVDPVVVE
                     NDLVIIAVVGERMRRTTGLAGRLFSALGEKSINVIAIAQGSSELNISIVIDQKDEKQA
                     LNAIHNAFFAKKIKMNLFLCGVGLIGSTLIEQIKTNYSNLLENYGLDIKIVGLANSRK
                     MLIEPDGIDLNNWRNSLNECQVKNDMRVLLAEIKKLNLDNNVFVDCTASADIVNYYEK
                     ILAGRTSIVTANKIGNTGPYAQYVKFHELARSKHVHFLYETNAGAALPIISTLRDMIN
                     SGDQIIRIEAILSGTISYILNNFKPGKKFSAIVREAKARGFTEPDPRDDLSGIDFARK
                     VLLLVREIGIPAELSDIQIEPLLPENCLKAPTVEAFFTELEKSNDYFDKLLQQASAEG
                     KVLRYIAIYENEKAELKLVQVEANHPFYFLSGSDNIVAFTTKRYAENPLVIKGAGAGA
                     EVTAAGVMADIFKIANAVMKSKSF"
     gene            10591..11529
                     /locus_tag="PLO58_06365"
     CDS             10591..11529
                     /locus_tag="PLO58_06365"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HQO74448.1"
                     /translation="MKSVTVFAPATVANVACGFDIFGFAVNEPGDVAKVTLRENPGVE
                     ITRITGDNGKLPKAAEKNTAGFAALRYLETIQSQQGVSIELHKKMPLSSGLGSSAASA
                     VAVLFAINTLFDNRLTKSELLSIALQSEKLACGAAHADNAAPALFGGFLLIRENNPPD
                     IVELPVPENLYCTIIHPEIEINTADARNILAPNVPLKDAVKQWANTAGLVAGLYRKDL
                     TLISRSMNDYIIEPQRARLIPHFYEMKKAALDRGALGCSISGSGPSLFALSNSQEIAQ
                     KIAGSMSAVLANYKICNQIYISTINTEGPKIIAQEG"
     gene            11533..>12770
                     /gene="thrC"
                     /locus_tag="PLO58_06370"
     CDS             11533..>12770
                     /gene="thrC"
                     /locus_tag="PLO58_06370"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQO74449.1"
                     /translation="MLYYSTTDRSHKVSLKEAVLKGMPPDGGLYLPESLPVMPDKFFQ
                     DLPEMTFQSVSYEIAKALLGGDVPSDVLQKIIVEALNFETPLVAISEKIKTLELFHGP
                     TLAFKDVGARFMSRLMAYFIRNDCRELTILVATSGDTGSAVANGFFQVPGINVIVLYP
                     AGKISLIQEKQIATLGENITALKVEGTFDDCQRLVKTALVDPEVLSVLTVSSANSINI
                     ARLIPQTFYYFNAIRQIPNFHSRKIAICVPSGNFGNLTAGLIAKKMGLPVIRFIAATN
                     INHVVPDYLATGKYEPRASIQTISNAMDVGNPSNFARMLALYDYSVEKMRRDLAGCYF
                     NDDETRAAIAEVYRNYGYILDPHGAVGYLGIQNYLAKHPEIDTGIFLETAHPAKFCDI
                     VEPVIGATVPIPDRLKKFLER"
BASE COUNT         3598 a         2992 c         2691 g         3389 t
ORIGIN      
        1 attaaaagtc aagagttgta ttacaaattt tgaaatgttt aacatctttt gtcgcggtca
       61 cgacctaatc tctattgttc ttcttccaaa agtcaaatat aatggattca acttaaagga
      121 ttttcggaat ttaaattgtc ccctaaacta ctaaggtcag atttcgtttt cacttcccgg
      181 ttttaaataa acggtacgca ccggataggg tatctcgata ttttcctctt taaagcgccg
      241 atgaatccgt ttgattagtt catgctggac gggaaactga acctcgtatt tccgcacttt
      301 gacaatcaaa ttgagactaa cgctactttc attaaaacga ttaaagcgca cgataggttc
      361 gctatctttt acacctccct caacttcgtt taaaacttct ctggcagtct ggattaaaac
      421 ttcttcaact ttatttagat cattgttata gccgaccaat accggaacta tgagagacat
      481 ttttttatct ggacggctat aattagtgat aattgtcttg gacagctctg aattaggaat
      541 aacattctcg ttgttcgcca tagttcggat aatcgtattg cgccagttga tatctttaac
      601 aaatccttct accccgctcg acaacattat ataatcatcc ggctgaacca acctcgtacc
      661 gataataaaa ataccggcaa aaagattaga aagcgtgtcc tgtagtgcca atgccaccgc
      721 caagccgccg actcctaaag ccgttagcaa tggcgtaatt gaaacaccta gtgtttgtag
      781 aattatcaaa aatcctatta cgtagatgac aatttttgtc aggttaataa agagagtcag
      841 agaaggcatt tttttacggc gaccaagatt ttcgataaaa ccaaccgcaa tccgtgccag
      901 caaaatggta aacgtcaaaa taatcagcac tattagtaac ttgcgcaagt tatttagaac
      961 ttcttcctca attccaaaat aacttaaagc aaaatacaag cccaccaatc ccatcacgat
     1021 tatcaaaatg ccacgcattg aattaccaac aacccttaac accgggggtc tgattttttg
     1081 tacgtttttc ctccatttac taaacactaa cctatccagc agtaatccga ttaaggcact
     1141 aattattact attgctaatg gtaaaatgta agttgcaatc gacttgggca ttttctatcc
     1201 ttttaatttt ttaataaaaa cgtttgattc agctcgcggc actctcttat ctcattccca
     1261 attacctcta ataataacga ttccgcatct ttatttcaag aataatatcc taaaaaacgc
     1321 tttctctact ttatatcata gtaccaaata aaattacaca caattaattg tctaaaatgc
     1381 acttaaaatc ttgatagttg acttttattg cttcaatcat aaaaaattat ttgcatatat
     1441 gtacggcgga ttgtatattt attcgctttt taagtatatt tatacgtttg aagatgaata
     1501 ctaagagaga ccgacacaga gctatccggc aaattattgc ctcccggcaa atttccaatc
     1561 agaacgaact aaaagacgtt ctcgaaaaac agggttttgt cacgactcag gcaactcttt
     1621 cgcgtgattt aatgactttg aaaatcatca aggtccctga cgccgaaaga gggtatattt
     1681 atgcgctttc cgaaaacttt caaagttcaa agccgccaat cgagggcagt tcgcccttgc
     1741 aaacctgccg atcgatcgcc ttttccggca atctcgccgt gctcaaatgc ctgccttctt
     1801 ttgctcccag tgtcgctctg cttctcgatg cctatgaaat gaatgaaata atcggcacaa
     1861 ttgccggtga tgataccgtt ctaattattc taaaagaagg attgtctcac gagcgctttc
     1921 gatcggcgct cgtggaacgc tgcccggaat tacgtaatcg aatttagacc gggattttta
     1981 taaagttaac cttaacaaat acatctatcc ggtagaatca actattttga aaggaattaa
     2041 tcaatgtcaa aaaaagtaat attagcatac agtggaggtc tcgacacctc tgtaatcctc
     2101 aaatggctag tcaataaagg ctacgacgta atttgctacg tcgccaatgt cggtcagcgg
     2161 gaagatttcg ccgccgtcga gcaaaaagcc ttcaaaaccg gcgcttcgaa agtctatatc
     2221 gaagacctgc gtcgtgaatt cgtgaatgac tatattttcg cggctttgcg tggcaatgcc
     2281 atttacgaag ggcgctattt attgggcaca tctctggcgc gtcctttaat tgcccagcgg
     2341 caagtcgaga tcgcccagat ggaaaaaact cattactacg ctcatggtgc gaccggcaag
     2401 ggtaatgacc aggtccgttt cgaattcgct tactatgccc ttgacacgga agcgattgtt
     2461 atttctccct ggaaagatcc ggaatttctc gccgaattca aaggtcgcag tgatttgatc
     2521 agatatgcgg aaaagtggaa tatacccgtc aaagcttcct taacaaaatc ctacagtgaa
     2581 gatgaaaacc tgatgcacat tagccacgaa gccggtgtcc ttgaagatcc ctctttccga
     2641 ccgccggaaa atgtcttcag ctggactgtt tcgccgcaag acgctccgga tagcgagacg
     2701 attctggaaa tagaattcaa ggacggttac ccgattaaag ttacaaatct aaacaacggc
     2761 gtcgtcaaag aagactcctt ggaacttttt tattacctta atgaaattgg gagtcagaac
     2821 ggcgtcgggc gagttgatat ggtcgagaac cgcttcgtcg gcattaagtc gcgcggcgtc
     2881 tatgaaactc ccggcgcaac gattttatgg acggcgcacc gcgacctgga aggtattgct
     2941 atggataagg aagtaatgca cctgcgggat atgctttcgc ctaaatttgc cgaattaatc
     3001 tataacggct tctggttttc acctgaaatg gattttctaa tgaatgcttt cgagaaaagt
     3061 caggaaaaga ttgacggctc agttacagtt tccatataca aaggcaatgt tactcccatc
     3121 ggacgtcgtt cgccgatttc gctctacgat caggctctat cgagtatgga catcgcaggt
     3181 ggtttcaatg ctaatgacag tctgggattt attcgcgtca atgctcttcg tctgaaagcc
     3241 cataaaatag tcctcaaaaa gaaaaaacct tacaactggc gcatcaagga ctaacctatg
     3301 aaactctggc aaaaaaaata tgatctcaac gccgccgtcg aaaaattcac cgtcggcaac
     3361 gactacctgc tcgatcaaga actcgttccg ttcgactgtc aggcttcccg agctcacgct
     3421 cgaatgctga ataaaatcgg cattctctcg gactcggaaa cggccgattt gctcagtgga
     3481 attgatcaaa tcctcgattt acacgccaaa ggcaaattta aaattcgccc cgaagacgaa
     3541 gattgccaca cggcgatcga aaattttctg acggaaaaaa tcggcatagc cggggaaaaa
     3601 atccataccg gtcgcagtcg taatgaccag gttctaaccg ccttacgtct ttactgcaaa
     3661 actcaattaa acgagatttc ctctgccgtt caaactacta tcgcgactat caccgagttt
     3721 atccaaaaat atggcgaggt taagatgccc ggttttacgc atacgcgcaa agcgatgcct
     3781 tccagcgtag caatgtgggc tggtgccctt cgagatgccc ttgccgatga ccttgatttg
     3841 ctagcctgtg ctacgaaatt gattgatcaa aatccgctcg gcaccggcgc tggatttggt
     3901 gttccgctag ctctcgaccg ccaaatgaca actgacgagt tacatttcgc ccgtattcaa
     3961 gaaaatccga tttacgccca gaacagtcgc ggcaaattcg agggttttgc tcttagtgta
     4021 atgtcgcaaa ttatgtatga tttaaacaaa gttgcctccg atctcatttt attcaccatg
     4081 ccggagttta attatttcac tctgccggat gaatttctaa ccggctcatc cattatgccg
     4141 cataagaaaa atccggacgt gcttgaatta ttgcgggcta aatatcacga ggttttcgct
     4201 tatgaaatgc aggttcgcaa taccggcgtc aacctgattt ccggctatca tcgcgacctg
     4261 caattgacca aagaagccat catccgagct tttaaaacga ccctaacgtc ggttcaaatc
     4321 actactgttt tatttgaaaa tttatcggtt aaccgtaaaa actgccggaa agctatgacc
     4381 gccgaattat ttgctaccga taaggtttat caattagtaa aaagcggcgt gcctttccgc
     4441 gaagcttacc gtcaaatcag tcaggaatat gaggataaaa atgaaaaaca atgctaaaat
     4501 tcgtgtatct gtagtcggtg ctaccggcta cgccggtttc gaactggtaa aaattctact
     4561 gcgtcacccg tacgtaacaa tcaaccgttt ggcagtaatt gcnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nntacaataa tcctaaaatc accgacccca aaggttttac
     4741 cggtgcggcg atgcatgccg gcttcaagcg caaacgcaat gacctgtgta taatcaaaag
     4801 caccgtgccg gctaaatgtt cagccaaatt caccaccaat gccgtcaaag ccgcgccgct
     4861 tatcgtcgct atggaaaatt taccgaaatc cggcaatcgc cttcaggctt tgatcatcaa
     4921 ttccggtaat gctaatgccg gtaccggtca gcagggtttg aacgacgtgc acgagactct
     4981 aaacttactt tccgcccagc ttgatattcc ggaggaacta attctggctt ccagcactgg
     5041 tgtaatcggc cattgcctcg atatggaaaa aatgcgaaat ggcattaccc acctcgtacc
     5101 gcatcttagt atagaaggtg gtgccgcctg cgccgaagcg atattgacga ccgataccgt
     5161 cgaaaaatcg ttcggtgtaa aattcagagt caatggcgcg gaaatcaagc tttgcggcat
     5221 taccaaaggt tccggtatga ttcagcctaa tatggcgacc acactggctt ttgtcaccac
     5281 tgatgccgat attgaccaca attttctgga aaaaactttc accacgactg tcgcaaaaac
     5341 ttacaatatg atcactgttg acggtcacac cagcaccaac gatactgccc tgattatggc
     5401 gaacggttta gcgggtaatc aaacaattac cgccggttcg ccagctggtg aaattttcga
     5461 aaatgctctc ctaatgctga tgaccgaaat ggcaaaatcc attgtccgag acggcgaagg
     5521 cgccaccaag ttcgtgaccg ttactgtcaa tggcgccgat agttttgaag atgctaaaaa
     5581 aatggcgttc ggtattgcta attccatgct ggtcaaagcc gcgctttttg gatgcgaccc
     5641 gaattggggc agaattctct ccgcggcggg gatggtattt gcccattttg atccggctgt
     5701 cgctgattta cgtatcaatg ggatttacct ctaccggcag ggtgaaccgc ttaatttgaa
     5761 taaaaccgaa attcaacgta tgatgacacc ggtcgaactg gagattgtac tcgactgcaa
     5821 cctcggaaaa gatagcgccg tagtttacac taccgacctt tcttatgatt atatcaaaat
     5881 taacgctcaa tatcatactt aggagctgtt atgcagaaaa ttattgacaa agccgccgtt
     5941 ttgatagaag ctttaccttt catcaaagcc tttcacggca agaaaatcgt gatcaaattc
     6001 ggcggcagta taatggacag tgaaaatgct atgaaatctg ttttgcagga cattgtcttt
     6061 atgaaatacg tcggctggtt accagtagtt atccacggcg gcggcaaagc cattagcgag
     6121 gcaatgaata accgtggagt taaaccgcaa ttcgttaatg gactgcgcgt aaccgaccgc
     6181 aaaactatgg aaatcgttga aaatgttctc gcccacaaaa tcaatgccca aattgttaac
     6241 ttgctgaata tgatggacgg cgaggcggtt ggagtttccg gtctcgataa cggattaata
     6301 cgcgttgata aaatgttcac cgaaaacaat gggcaatcgg tagacctcgg tttcgttggt
     6361 caggttcgct acattaatgc taattttctt aatcggctta tagaaagcgg aaaaattcct
     6421 gtaattgcac cactcggttt gggtgacgat ggcgagagct acaatatcaa tgccgacctt
     6481 gttgccggcg aaatcgcggc ttcaattggc gctcgcaagc tagtttattt aactgatacc
     6541 cctggcattt tgcggacacc cggcgacgag aaaagtatca ttagtaccat taatcgccag
     6601 gaggctgaaa aacttatcgc cgcgggcatc atcgagggcg gtatgattcc caaagtcaac
     6661 tcctgtctcg atgctttgag gtttggggtc aacaaaggtc acattataga cgggcgcatt
     6721 aagcactcgc tcttactcga aattttcact aaagaaggaa taggaacgga gatattaaaa
     6781 gatggatcgg aaagaattcg ataattacgt tttacctact tataagcgtt tcccgattac
     6841 cctggtaaaa ggacgcggta tgcaggtctg ggatgatgaa ggcaacgagt acctcgactt
     6901 tttccccggt tggggttgtg gcaatatcgg gcattgccat cccaaagtcg tggcggctct
     6961 gcatgaacaa ttggacaaaa tcatgcacgt accgaatgtc tattataacg agaaagccgg
     7021 cgaacttgcc aaactaatca ttcaggacag ctttgatgga caagttttct tcggcaacag
     7081 cggcgcggaa gccaatgaag gagcactgaa gctggtgcgc aaacgcaacc ccaataagaa
     7141 aacgattatt acgctgaata actcctttca tggacgcacg ctggctacag ttaccgcaac
     7201 cggtcagagc gaataccaga aaggctttga tcccctgctc ggcggttttg tctattgcga
     7261 tatcaatgat tacgctatgc tcgcaagctt aatgaacgaa aacgtcgcgg ctattatgct
     7321 cgaagtcgtc cagggcgaag gcggcattac acctgtcaca ccaaaattta ttcagacagc
     7381 gcgcgaactg tgcgaccggt ttgacgctat gctaatcata gatgaagttc agaccggcat
     7441 cgggcgcacc ggtaaaatgt ttgcctacca atatttcgat gtccaaccag acgttatgac
     7501 tctcgccaaa tcactcggcg gcggtatgcc gatcggcgct tttgccgtag cacgccaata
     7561 ttgcaatatt ttacaacccg gcagtcacgg ctcgactttc ggtggtaatc cactggcttg
     7621 cgccgctggt gtggcggttt ttcaagctat tcacgaagaa aatctgctcg agaatgccgt
     7681 tgaaatgggc acctatttcc ggcaaaaatt ggaagaactg aaaagccaag cgccggtaat
     7741 tcgtgaaatc cgcgggctcg gacttatgct gggcgtcgaa ttaaataagc ccggcgcgga
     7801 aatcgttgac aagtgccgcg cccagaatct actgattaat tgcacacacc agaccgttct
     7861 gcgcctgatg cccgccatca acgtcactaa agagcagatc gataaagctg ttgcgattat
     7921 cgggaaagcc ttgatggaat cataggcaaa accttccgaa ggttgggtta ggctatttga
     7981 agctatctga aggttttatc aattagtttc gtgtttaatc cgtagttaaa tctatctctg
     8041 tatttggctt ggcaggttct taaaaaaatc gtattttctc tcgctataat tgaacaattg
     8101 acaaccagat agaaagacag ttatgaaagt cttaaaattc ggcggcagtt cgattggtaa
     8161 gccagaaaat ctgccaacta ttttcaatat catcgccagg gaagtccaga gcgagaaggc
     8221 ggtaattaca gttttttcag ccttcaataa tgtcactgat caattgaaag aaatcagcca
     8281 attagcggca agcaaagata aaacctatca aaatattctt gatgaccttg cccgaaaaca
     8341 ttttaatttt atcgaaaaat taatacccca agataagcag aagccactca acgccgaaat
     8401 caaaaacctc ctggcagaat tgaaagaaat tatccacggc gttttcttat tgaaagaatt
     8461 atccttgcaa tctctcgacc tgatcctgag tttcggagaa cgcttggctt gcaccatcat
     8521 cgccgcctat ggtcaatcca tcggttaccc aacaaagtac gtggatgcgc gccaatttct
     8581 ggtaaccgat gatcatttcg gagcggcgcg tattcttccg cacatcagca atccgaaaat
     8641 ccaggcattc tttaaaaatt tcactggaat tccagtagtc accggtttca tcggcgctac
     8701 cgcagatggc attactacaa ctttaggccg cggcggttcg gacttaactg cctctcaaat
     8761 cggtgcggct cttaacgcgg aggagattca aatctggaaa gacgtcaatg gctttatgtc
     8821 ggctgatcca agcaaagtca aagaagcttt ttcattagcg gaactgagct atgaagaagc
     8881 tgtggaactc agtcatttcg gcgccaaagt actatttcca cccactattc agccggtaat
     8941 tgataagaat attcctattc gtataatgaa tactttcaat ccggattttc ccggaacggt
     9001 cattcgcaaa aatccctccg ccaacggtgg ctacgtcaaa ggtgttactt caatcagcga
     9061 catttcattg ttgacagttc aaggtagtgg catggttggc gttagtggca tctcttctcg
     9121 tcttttcagc gctctggcac aagtcaacgt caacgtgatt ttgatttccc aagcctcgtc
     9181 tgaacattcc atctgtgtcg ccattttgcg agaatctgtc aatatcgcaa aaaaagccat
     9241 cgaagaagaa ttcgctcttg aaattaaact ccgcttggtt gacccggtcg tagtagaaaa
     9301 tgatttagtc atcattgcgg ttgttggaga gcgaatgcgt cgcactaccg gcttggcggg
     9361 acgtcttttt agcgcattgg gagaaaaatc cattaacgtc attgccatcg ctcaaggttc
     9421 ctcggaactg aatatttcca tcgtaattga ccaaaaagat gaaaaacaag cccttaatgc
     9481 cattcataat gctttcttcg ctaaaaagat taaaatgaat ttatttctct gcggggtggg
     9541 tttaatcgga tcaactttaa tcgaacagat taaaaccaat tattccaatc tactggaaaa
     9601 ttatgggctg gatattaaaa tcgtcggtct tgctaatagc cggaaaatgc taatcgagcc
     9661 cgacggaatt gacctcaaca attggcgcaa ttctctcaat gagtgtcagg tcaaaaacga
     9721 tatgcgggtt ttactggcag aaataaaaaa actcaatctc gacaacaatg tcttcgtaga
     9781 ttgcaccgcc agcgccgata tcgtgaatta ttacgaaaaa attttagccg gtcgcacctc
     9841 catagtaact gctaataaaa tcggcaacac tggcccatac gctcaatatg taaaattcca
     9901 cgaactggcg agaagtaaac acgtccattt tctctacgaa acaaatgccg gtgcggctct
     9961 gccaattatc agcactctgc gcgatatgat caattccggc gaccaaatca tccgaatcga
    10021 agcaattctt tctggcacaa ttagttatat tctcaataat ttcaaaccgg ggaaaaagtt
    10081 cagcgccatt gtgcgcgaag ccaaagccag aggttttacc gaacccgatc cacgcgacga
    10141 tcttagtggg atagatttcg cccgcaaggt tttactgctc gtccgcgaaa tcggcattcc
    10201 cgccgaactc tccgacattc aaatcgaacc gcttttgccg gaaaattgtc tcaaagctcc
    10261 gacggttgaa gcgtttttca ccgaactcga aaaatccaat gactattttg ataaattgct
    10321 ccagcaagcc tctgcggaag gtaaagtatt acgctatatc gcaatttatg aaaatgaaaa
    10381 agctgaattg aaattagttc aggtagaagc gaatcatccc ttctacttct tgagcggtag
    10441 tgacaacatc gtcgctttca ctacgaaacg ctacgctgaa aatccgttgg ttatcaaagg
    10501 cgcgggggct ggggcggaag tcaccgccgc cggtgtaatg gctgacattt ttaaaattgc
    10561 caacgccgtt atgaaaagca agagtttttg atgaagtctg tcactgtttt cgcaccggct
    10621 actgtggcta atgttgcttg cggtttcgat attttcggtt ttgctgttaa tgaaccgggc
    10681 gacgttgcta aggttactct ccgcgaaaat cccggcgtcg aaattacacg cataaccggc
    10741 gacaacggga aattgccaaa agccgccgaa aaaaacaccg ccggattcgc cgccctccgt
    10801 tatctcgaaa ccattcaatc ccagcagggt gtttccatcg aattacataa aaaaatgccc
    10861 ttgagtagtg gactgggatc gagcgccgcc agtgcggtgg cggtactctt tgccattaac
    10921 actcttttcg ataatcggct gacgaaatcc gaactgctca gtatcgctct gcaatcggaa
    10981 aaattggctt gcggcgccgc ccatgccgac aacgccgcac cggctctctt tggcggtttt
    11041 cttttaattc gggaaaacaa tccacctgat atcgtagaat taccggtccc ggaaaatctc
    11101 tattgcacaa ttattcatcc agaaattgaa atcaatactg ccgatgcccg taatattctt
    11161 gctcccaatg ttcctctaaa agacgccgta aaacaatggg ctaacactgc cggactggtt
    11221 gccggtctct atcggaaaga cctaacattg attagccgct caatgaatga ttatattatc
    11281 gaacctcagc gggcgagact tattcctcat ttttatgaga tgaaaaaggc ggctctggac
    11341 cgcggcgctc tcgggtgcag tatttccggc tccggtccgt cgctttttgc gctttcaaac
    11401 tcacaagaaa ttgctcaaaa aatcgccggc tctatgtcag ccgtcttagc taattacaaa
    11461 atctgcaacc aaatctatat ctcgacgatt aacaccgaag gtcccaaaat catcgctcaa
    11521 gaaggataaa atttgcttta ttacagtact actgatcgct ctcacaaagt ttctctgaaa
    11581 gaagccgtgt tgaaaggcat gccaccggat ggcgggctat atctgccgga atcattgccg
    11641 gttatgccgg acaaattttt ccaagacttg ccggaaatga cttttcagtc ggtgtcttat
    11701 gaaattgcta aagctctact tgggggcgat gtcccatctg atgtccttca aaaaattatc
    11761 gttgaagctt taaatttcga aacaccactg gtggctattt cggaaaagat taaaacactc
    11821 gaactttttc acggaccaac tctcgccttt aaagatgtcg gcgctcgttt tatgtcgcgc
    11881 ctgatggctt attttattcg taatgactgt cgcgaactca cgatcttagt tgccacctcc
    11941 ggtgataccg gcagtgcggt tgctaatggt tttttccagg tgccgggcat caatgtcatc
    12001 gtgctttatc cagccggaaa aattagccta attcaagaaa aacagattgc gactctcggc
    12061 gagaatatta ctgccttaaa agtagaaggc accttcgacg actgccagcg tcttgtcaaa
    12121 accgctctgg tggatccgga ggtgcttagt gtgcttaccg tcagttccgc caattcgatc
    12181 aacatcgccc gtttaattcc tcaaaccttt tattatttca acgcaattcg ccagattccc
    12241 aattttcatt ctaggaaaat cgctatctgc gtaccgagcg gcaatttcgg taacctgacc
    12301 gccggattaa tcgctaaaaa aatgggctta ccggtaatac gcttcatcgc ggctaccaat
    12361 atcaatcacg tcgtgccaga ttatctagcg accggaaaat atgaaccacg cgcctcaatt
    12421 cagactatct ccaacgctat ggacgttggc aatcccagca atttcgcccg gatgctggcg
    12481 ctctatgatt attcggttga gaaaatgcgt cgcgatttgg ctggctgtta ttttaatgac
    12541 gatgaaactc gcgccgccat cgccgaagtc tatcgcaatt acggttatat tctcgaccca
    12601 catggtgccg tcggctacct tggcattcaa aattatctcg ccaaacatcc agaaattgac
    12661 accggaattt ttctggaaac agctcacccg gcgaagttct gcgacattgt cgaaccagtt
    12721 atcggagcaa cagttcccat ccccgaccga ctgaaaaaat tcctggagcg
//
LOCUS       DAOMJU010000055        12668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_4995_length_12668_cov_22.723698, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000055 DAOMJU010000000
VERSION     DAOMJU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12668
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4995_length_12668_cov_22.723698"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2354)
                     /locus_tag="PLO58_06375"
     CDS             complement(<1..2354)
                     /locus_tag="PLO58_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HQO74450.1"
                     /translation="MKKRSCLELILTICILVTATVQAQTLAFVNWKCAPPDSQKVSAT
                     AGNLLGLPQIGAPGFVVRDYKEGPGPDQRWWPYENGSATYWGAESLQVDTRWVQFAAY
                     PKENITFQADSFTCFIGAKGTGEMRANVWYATDTSFADAIQINDSVLVLAKDNHIFYN
                     FAIDTTITSADTLFIRIYPWYQGSSSNSKYLYLLNATIWGTTEGEFVANPPTVTTAPV
                     TDISTTFAISGGTVTDDGGAPVTARGVCWNTTSMPDTSDNKTNDGESSGAFVSLITGL
                     EPGTTYYVRAYASNSAGIGYGEEVTFKTLDSLEVPAVETAKVTNIMVKTATCGGNVTA
                     WGGDTVRVRGVCWNTTGNPTIADSKTENGSGLGTFQSILYPLTENTTYYVRAYATNSK
                     GTGYGEVDSFTTQTPAPDVLKIVAADGSGDYPTVQAAFDDVPDYYTGRYIIYVKKGVY
                     HEKLFLDRYKMNVILIGEDRDSTILTYDDYAGKAGGTSNSYSVAIDADDFVAMNITFQ
                     NTVKNDGTFSDQQAVALRVNGDRQAYYNCNLLGYQDTYYLWGAWGNRVTGRVYMHKCY
                     IEGSVDFIFGRDIAVFDSCHINVNRNAGTITAASTEPGAKFGLVFRDCIITADEIGFN
                     ELPIGNIYLGRPWAAAPRTVFIKCEEPEVLNPGGWLEWNVVPALYAEYQCTGPGSNTS
                     SRIAISRQLTDEEAQEYTLENIFAKTSYPTIAYDWLPVKPNVNIPGVAVKPDANVPLT
                     FNLGQNYPNPFNPTTTIRYELAQPGKVSLTVHNLLGQKVLTVVDT"
     gene            complement(2398..3903)
                     /locus_tag="PLO58_06380"
     CDS             complement(2398..3903)
                     /locus_tag="PLO58_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HQO74451.1"
                     /translation="MKPKINFTLGRFLIFLTAAALTIIIYAAHNKPRVHLIGDSTMAD
                     KPLDDNPERGWGQLFPLYFTQEIQIINYARNGRSTKSFIDEGLWANVYNNLKPGDFVF
                     IQFGHNDAKFSDTTRYAPARTTYRANLVRFIEESRGKGAYPILITPVNRRKFNEAGEF
                     VDQHGDYPVVVRELAAEYDAPLIDLHARSRELFSQLGAEKSAELFLRVPAGVYKHYPD
                     GKSDNTHFNRYGAIRICELVIEEMKAIDLPLVKYLKDGPELSDMGVGKVVGLDYFYNH
                     EFKNGNQYHYTWEDRENSGFYELGNLFENCGAFIAEVCAEPTPEELARLSIYIIVDPD
                     TPQETTMPNYISEKAIKTITNWVESGGVLVLLGNDAGNAEFEHFNRLAQNFGIHFNEV
                     SLNRVTNKNYEMGKFDNLPDHPLFENITKIYMKEVASLTLVGNAEPILMSGGNVIIAS
                     SKYGQGLVLAIGDPWLYNEYIDNRILPADFENYSAALNFCKWLLNQSRPVR"
     gene            complement(3909..5567)
                     /locus_tag="PLO58_06385"
     CDS             complement(3909..5567)
                     /locus_tag="PLO58_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HQO74452.1"
                     /translation="MIIKKMMSSRHQRSCFHQRIFLIGFGLLTILAVNPLGAELPDLT
                     YSNPIIQADYSDPDVIRVGDDFYLISSSFSQAPGVPVLHSRNLVNWQIIGYAVPHLPD
                     PVYDLPQPGRGIWAPALRYHNGEYWVYYGDPDLGIFMVKAQNPAGPWEPPVLVERAYG
                     WIDPCPFWDDDGQAYLIHAWAKSRVGFNSILTLHKLSSDGRQILDEGVTVFDGHSTHP
                     TIEGPKLYKRNGYYYIFAPAGGVTNGWQTGLRSKNILGPYEDKIVLEQGTTAINGPHQ
                     GAWIETNQGESWFIHFQDKGAYGRILHLQPVEWLNDWPMIGIDLDGNGIGEPVQCFRR
                     PDVDNPISAFQMQTSDEFESNSLGLQWQWRANFKSEWFTLSARSGYLRLFTVNPAPVC
                     YNLGDLPNILTQKFPAEAFTVTTKLSFHPDSNDDQAGLIIIGNDYAALKVTHRGARYQ
                     LTYAECTEIEKGKSEIISETVGLNKPEVFLKANVGEGAVCTFSYSTDGEKYLPLGRKF
                     QAVKGKWVGSQIGIFATTTPGKISSGYADFDFFRVMSIGKDEPK"
     gene            complement(5564..6580)
                     /locus_tag="PLO58_06390"
     CDS             complement(5564..6580)
                     /locus_tag="PLO58_06390"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO74453.1"
                     /translation="MKKLIYLTITCILLLSLSSPINAQVGLKKVAQSTMNFLQVGVAP
                     RASAMGEAYCAFGTGAESIFFNPAGIAQSTHRFELQTYNTRWIADINYLAGSFIWNLR
                     QYGTLGLSMLSVDYGTIYGTSLLSAADKDLYPAGYIDLGNVDNVGSYAFGLTYGRAIS
                     TQFLIGGTVRLVGQNLGQSQMADGLKDNNAVKLAFDAGVKYLTDFKSFRFAMSIRNFS
                     SNIKREEIDEQLPLLFTMGVAMDLLDVIAPTFSQNNSLNLAVDFQHPNNYSERINYGL
                     EYLLFDQFAIRAGYQTNQDLASWSLGMGINVNIIGNTVQFDYSYSDFDLFDGVNRFSI
                     GIAL"
     gene            complement(6598..>7820)
                     /locus_tag="PLO58_06395"
     CDS             complement(6598..>7820)
                     /locus_tag="PLO58_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74454.1"
                     /translation="PKVTYIGTATKIPYNDASDEYGNKNYWAIRGGYSKYWPMIGDTI
                     PGTFHGGNSDENGSSDYSDFPAGTKSSPNSKMSCSFGPYTFAPGQKIHIVYASGYSGL
                     DLPTAQEVGKKWLNGTLEDPPNLPDSHTGYLPSNFVFPIDATEMDLRKDRWVSTGIDS
                     VMKSARRAKWNFDNGYKIPQAPPPPTYLEITGLGTGVEIKWRDPDAEAMPNFAGYRIM
                     RRLSAMDTTFYQVIYDSGPDDKATDHLYIDRDVLIGAQYYYYLQAKARIADDDPLADA
                     TTRGKIIYSSRVLVPNINWINPPHFSQTDLTKIRIVPNPYNINDPLLAAQGWTDQRGI
                     QFYNLPGKVTIKIFTENGDLVQVLKHDSPVEAGYYFWDMITLSQQVISSGVYIVVFQK
                     PNGETAFQKFLVVR"
     assembly_gap    7821..7920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7921..8915)
                     /locus_tag="PLO58_06400"
     CDS             complement(<7921..8915)
                     /locus_tag="PLO58_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74455.1"
                     /translation="MKNYRKNNLRINFSLILLAILCAGQFGFSQIQPELATALIKMGH
                     VWCGVTANGDKGSFEYRAGFFPNDYDIIAIRGQELDAWAGAGFRLAATNWQDPVYPDS
                     IHKVAIYGPTNDYMRTGKVIVPLTNYVRYTYPEQIINFNPINLEMFGTYDPSKFGEET
                     YDQIVEVTTQNILGVNIHRKILAWTQNYNDDYIIVDVTFTNVSEDTLHDFFINMESNG
                     VNAFRSNGSNPSPPSNERFNPTIVWQHYYGGRVGDSLRVFYEYSADDPTIAGDNMGAP
                     VTAQGGRLIGAKFCWYSILHASGEPYDDRTNDRDDFTQPKVTYIGTATKIPYNDA"
     gene            complement(8943..12248)
                     /locus_tag="PLO58_06405"
     CDS             complement(8943..12248)
                     /locus_tag="PLO58_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO74456.1"
                     /translation="MLNNKPIIFFFCAALILTVGFTSNLQGQNRSRISGSVRDAETGE
                     PLIGVNIIIEETDLGAATDTDGNYIIINVPVGTHRVRASMIGYETKIFTNVMVSADRI
                     TRLDFELHRTVIQGQEVIVTAKRDELHKEVSTTQMVVDAMQISDATGIREINAFLAKL
                     PGIGEQNGYLTIRGGSADQTGMMVDGLSYVNAATGNAESSIPLSAIEQVSLLSGGYNA
                     EYGNFRSGLINITTKSGSSKDYHGTFSYSRDNAHIRRFGDSFYDAHNAALRSYLDPEV
                     AFIGTNTAWKDDAYLRDQYPSFTGWITQANNYNIGKTEDQWATPFDYYLLACWMFMTI
                     PDYKGLANLSDSVKQVIGYHEVSKEQKKLFAQHHNREEYPDWNFDGGFGGPVPLVGKA
                     LGNATFYLSFNAKERHFVMPVSRNSDETYTTLLTMKSQPTKKTTLTYNGLYKYEDGVS
                     PIRPASGDYPDASRQGGFMPVNNIKYIHKNPEYWYDPPFFPIMSQRTAVNGVTVNHII
                     NKTTFWELKLSALQIRDNSPTGNTRDMRAITHFGPFVVNEMPYGKLLDGTNRVTGIFN
                     GDTLSYKFPNYDALPGVAYRFRRKEGDLYNNVRVGQYGAKFDLSSQIGRHNFVKTGFE
                     YNYIDLDHNLWMMWNENAYNVYEFNYHRTPSQTGIYVQDQLNFESMIANIGLRFDYYY
                     GGGGKWPTGDPFATEAFTPQSYASDSVLFDYLKRGKSYIWDTWVRYDSLHPGFLQPIK
                     NHYALSPRIGISFPVTEQSKFYFNYGHFRSNPPYYSMYLYRYRYTKNGLYDMSNPNLE
                     PPRTISYELGIAMTLFQTSVLKMSWYSKDVTGQHGEVTYQNASGTINYNAWANNEYQD
                     IQGIEINFSKSDASWLTGWINFNYMLKKTGLTGRSLITDVTINNDQEGLYAGQETRSL
                     PQPKINANLTVRSFDKWGPKISGIYPLADWTITLFAEWQAGEYFTWNPLNELHVNNNM
                     RWPDYYMVDLKVDKKFNLFGLTASFYVDISNLFNFKTTLMDDGYCFVREDGDLEEWSD
                     FVKYFGSLRLPMYESAKYDALRQQYPGMYIAGNDQIGELRSKEKPYINDPNYSFWLYE
                     QPRDIWFGLRFSF"
     gene            complement(12291..>12668)
                     /locus_tag="PLO58_06410"
     CDS             complement(12291..>12668)
                     /locus_tag="PLO58_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO74457.1"
                     /translation="NLLDENGYTRLENYLNGLVEQNPNRIVSKPNVVADFRLAQNYPN
                     PFNATTTIQFSLFKTGLAEIRIYDLAGQVVKTYPRQSLMPGEYRLVWQGDNDAGKPVA
                     SGIYFCVMQFASNRQILKMQIIR"
BASE COUNT         3364 a         2925 c         2921 g         3358 t
ORIGIN      
        1 gtatcaacga cagtcaggac tttttgaccc agcaaattat gcactgtcag gctaactttg
       61 cccggttggg caagttcata gcggatagtt gtcgtcggat tgaagggatt cgggtaattt
      121 tggccaagat taaaagttaa cggaacatta gcatctggtt tgacggcaac gcccggaata
      181 ttgacattcg gtttaaccgg tagccaatcg taggcgatcg tgggatagga agtcttggca
      241 aagatgtttt ccagagtata ttcttgagct tcctcatcgg tcagttgccg ggaaatggcg
      301 atgcggctag aggtattcga gcccggaccg gtacattgat attcggcgta aagcgccgga
      361 acaacattcc attccagcca gccacccgga ttcaggactt ccggttcttc acatttaata
      421 aaaaccgttc gtggtgccgc tgcccaaggg cgaccgagat agatattgcc aatcggcaat
      481 tcattgaacc cgatttcgtc ggcggtaatg atgcaatctc tgaagaccaa gccgaatttc
      541 gctcccggtt cggtactggc ggcggttatc gtgccggcat tgcgattgac attaatgtgg
      601 caggaatcga agacggcaat atcgcgcccg aagatgaaat ctaccgagcc ttcgatgtag
      661 catttgtgca tataaacacg accagtgacg cgattacccc aggcgcccca gagataataa
      721 gtgtcctggt aacccaacaa attacaatta taataagcct gccggtcgcc attgactctc
      781 agagccactg cttgttgatc gctgaatgtg ccgtcgtttt ttactgtatt ctggaaagtg
      841 atattcatcg ccacgaaatc atcggcatca atggcgacgc tataagaatt cgacgttccg
      901 ccggctttac cggcgtaatc gtcgtaggtc aaaatggtgc tgtcgcggtc ttcacctatg
      961 agaatgacgt tcattttata gcgatccagg aacaattttt cgtgataaac acctttttta
     1021 acataaataa tgtaacgacc ggtgtaataa tcgggcacat catcaaaggc cgcctgcacg
     1081 gttgggtaat ccccactgcc gtccgcggct acgattttca gcacatccgg agctggagtt
     1141 tgcgttgtga aagaatccac ttctccatag ccagtgcctt tactattggt ggcgtaagcg
     1201 cgcacataat aagttgtatt ttcggtcagc ggataaagaa tgctctggaa agtacccagc
     1261 ccgctgccgt tctcggtttt gctgtcggca atagtaggat tgccggttgt attccagcaa
     1321 acgccgcgga cgcgtaccgt atcgccaccc caggcagtta cgtttccgcc acaggtggca
     1381 gtttttacca ttatattggt tactttagcc gtctcaaccg ctggtacttc cagtgaatcc
     1441 agtgtcttaa aagtaacttc ttcgccataa cctattccag cgctattgct ggcatacgcc
     1501 cgaacgtaat aagtcgtgcc gggttccaat ccggtgatga ggctgacaaa agcgccactg
     1561 ctttccccgt cattggtctt gttgtcagaa gtgtccggca tgctggtcgt attccagcaa
     1621 acgccacggg cggtcaccgg cgcaccgccg tcatcggtga cagtaccgcc actgatagca
     1681 aaggttgtcg aaatatcagt cactggcgcc gttgttactg tcggaggatt cgccacaaat
     1741 tcgccttcgg tggtgcccca gatagtagca ttgagcaagt aaagatattt ggagtttgaa
     1801 gatgaaccct gataccaggg atagattcga atgaaaagag tatcggcgga tgtaatagtg
     1861 gtatcaatgg cgaagttgta gaaaatatga ttatccttgg caaggaccaa tacactgtca
     1921 tttatttgaa ttgcatccgc gaagctggta tcggttgcgt accagacatt agcacgcatt
     1981 tcaccggtac ctttggcgcc aatgaaacag gtgaaactgt ctgcctgaaa agtgatattc
     2041 tccttggggt aagccgcaaa ctgtacccag cgagtatcaa cctgaaggct ctctgctccc
     2101 cagtaggtcg cagatccgtt ttcataaggc caccagcgtt gatcgggtcc gggtccttct
     2161 ttataatccc ggacgacaaa gcccggcgcg ccgatctggg gtagtcccaa gagattgcca
     2221 gctgtggcgg agactttctg actatcaggt ggcgcacatt tccaatttac gaacgccagc
     2281 gtttgggctt gaacggtggc ggtgaccagt atgcagatag tcagaattaa ttctaggcaa
     2341 ctcctctttt tcatacatac ctcctgttta atcataaatt ttaaataacc aattcaatca
     2401 gcgaaccggt cttgactgat ttaataacca tttacaaaag ttcaaggcgg ctgaataatt
     2461 ctcgaaatca gcgggcagta tgcgattgtc aatgtactcg ttatatagcc aagggtcacc
     2521 aatagccaat accaatccct gaccgtactt cgaactggcg atgattacat tgccgccaga
     2581 catcagaatc ggttcggcat tccccactaa agtcaacgat gcgacttctt tcatataaat
     2641 tttggtaata ttctcaaata acggatgatc cggcagattg tcgaatttgc ccatttcgta
     2701 attcttattg gtaacccggt tgaggctgac ttcattgaag tgaatcccga aattctgtgc
     2761 cagccggtta aagtgttcga attcggcatt gccagcgtca ttgccgagaa ggactaatac
     2821 gccgccggat tccacccaat tcgtgatggt cttgattgct ttttcggaaa tataattcgg
     2881 catggtggtt tcttgcggcg tatccggatc aacgatgatg taaatgctaa gtcgagccag
     2941 ttcctctggc gtcggttcag cgcatacttc ggcaataaat gccccgcaat tttcgaacaa
     3001 attacccagt tcgtaaaacc cggagttctc acgatcttcc caggtgtaat ggtactgatt
     3061 gccgtttttg aattcgtgat tgtaaaagta gtccagaccg acaaccttcc cgacgcccat
     3121 atccgataac tccggtccat ctttgagata ctttaccagc ggcagatcaa tggctttcat
     3181 ttcctcgata actaattcgc aaatccggat tgcgccgtag cgattgaaat gcgtgttgtc
     3241 ggattttcca tcgggataat gtttatagac tcccgctggg acacgcagaa ataattccgc
     3301 agatttttct gcacccagct ggctaaatag ttcccgactg cgggcgtgta agtcaatcag
     3361 aggcgcgtca tattcagcgg ctaattcgcg gacaacgacc ggataatcac cgtgttggtc
     3421 aacgaattcg ccggcttcat tgaatttgcg gcgattgact ggagtaatca atatcggata
     3481 ggcgcccttg ccgcgtgatt cctcgatgaa tcgaactaaa ttggcgcggt aggttgttcg
     3541 ggcgggagcg tagcgagtcg tatcgctgaa cttggcgtca ttatgaccga attggataaa
     3601 aacaaaatca cctggcttta ggttattata aacattagcc cataatccct cgtcaataaa
     3661 actcttcgta ctgcggccgt tacgggcgta attgataatc tgaatctcct gcgtaaaata
     3721 cagcggaaaa agctgtcccc agccgcgttc gggattatcg tccagcggtt tatctgccat
     3781 ggtcgaatcg ccgatcaggt gaaccctagg cttattatgc gcggcatata taatgatcgt
     3841 cagagcggcg gcagttagaa aaattaaaaa cctgcccaat gtaaagttta tctttggctt
     3901 cataatgttc atttcggttc gtctttgcct attgacatta cgcgaaagaa gtcaaaatcg
     3961 gcatagccac tggaaatttt ccccggcgta gtcgtagcaa aaataccaat ttgagagccg
     4021 acccatttgc ctttgacggc ttggaatttt cggccgagcg gtaaatattt ttcgccatca
     4081 gtactgtagc taaatgtgca aaccgcacct tcgccgacgt tagcttttag gaatacttcc
     4141 ggcttattga ggccaacggt ttcgctaatt atttccgatt tgcctttctc gatttcggta
     4201 cattcggcgt aagtcagctg gtatctcgca ccgcggtgag ttactttgag tgcggcgtag
     4261 tcgttgccga taataatcag cccagcctga tcgtcgttac tatcaggatg gaaagataac
     4321 ttcgtggtaa ccgtgaacgc ttccgcgggg aatttttgag ttagaatatt gggtaaatcg
     4381 ccaagattat agcaaacagg cgccggattc acggtaaata atcgcagata accggatcga
     4441 gccgagagtg taaaccattc cgatttaaaa ttcgcgcgcc actgccattg aagcccgaga
     4501 ctattactct cgaattcatc gctggtctgc atctggaacg cagagattgg gttgtcaaca
     4561 tccggtcggc ggaagcattg aaccggttca ccaatcccat tgccgtccag atcaataccg
     4621 atcatcggcc aatcatttag ccattcaaca ggctgaagat gcaagatgcg gccgtaagcc
     4681 cctttatcct gaaaatgaat gaaccaggat tcgccttggt tcgtttcgat ccaagcccct
     4741 tgatgcggcc cgttgatggc tgtagtgccc tgctcgagaa cgattttgtc ttcgtaggga
     4801 cccaagatat tttttgaacg caagccagtc tgccagccgt tcgttacgcc accggctggc
     4861 gcaaaaatat agtaatatcc attacgctta taaagtttag gaccttcgat cgttgggtga
     4921 gtggaatgtc cgtcgaaaac ggtcacgcct tcgtccagaa tctggcgacc gtcggagctc
     4981 aatttatgca aagtcaagat actgttaaat cctacccgac tttttgccca ggcgtggatc
     5041 aaataagctt gaccgtcgtc atcccaaaaa ggacaaggat cgatccaacc ataagctcgt
     5101 tcgacgagaa ctggcggctc ccacgggcca gccggatttt gcgctttgac cataaaaata
     5161 cccagatcgg gatcaccgta atagacccag tactcgccgt tatggtaacg aagagccggc
     5221 gcccaaatgc ccctcccagg ctgaggtaaa tcatagactg gatccggcaa atgtgggaca
     5281 gcatatccaa tgatttgcca atttaccaga ttacgagaat gcagaacggg aacacctggc
     5341 gcctgggaaa acgaggacga aatcaaataa aagtcgtcac ctacccgaat aacgtccgga
     5401 tcggaatagt cggcctgaat tatcggattg gaataggtca aatcgggcaa ctcggcgccg
     5461 aggggattaa ctgccagaat cgtcaataat ccgaagccta tcagaaagat tctctggtga
     5521 aaacaacttc tctgatgacg tgaactcatc atttttttaa ttatcacaag gctattccta
     5581 ttgagaaacg attaacgcca tcaaataaat cgaagtcaga ataggaataa tcaaactgga
     5641 cggtattgcc gattatgttt acattgattc ccattccaag cgaccaagaa gccaggtctt
     5701 gattggtttg gtacccggcg cggatcgcaa attgatcgaa tagcaaatat tccagtccat
     5761 aattaatccg ttcggaatag ttattcggat gctgaaaatc aaccgctaaa ttgagagaat
     5821 tattttgcga gaatgtcggc gcaatgacat ccagcaaatc cattgctacc cccatcgtaa
     5881 agagcaacgg taattgttcg tctatttctt cccttttgat attagatgag aaattgcgaa
     5941 tggacatagc gaaccgaaag ctcttgaaat ctgtcaagta ttttaccccg gcatcgaacg
     6001 ccagtttaac cgcattatta tctttcaaac cgtccgccat ttgactttga cctaaatttt
     6061 gaccgacgag acgtacagtg ccgccgatta aaaactgggt gctgattgcg cgaccataag
     6121 ttagaccaaa cgcataggag ccgacattat caacattgcc caaatcaata tagcctgccg
     6181 gatagaggtc tttgtccgcc gccgacaaga ggctggtacc ataaattgtc ccgtaatcaa
     6241 ctgaaagcat actcaatccc agcgttccgt actgacgtaa attccagatg aagctaccag
     6301 ccaggtaatt gatgtctgca atccagcgtg tattgtaagt ttgcagttcg aaccgatggg
     6361 tagattgagc aataccggct ggattgaaaa aaatgctttc cgcgccggtt ccgaaagcgc
     6421 aataggcttc gcccatagcg ctggcacgcg gtgcgacacc cacctgtaaa aagttcatcg
     6481 tactctgcgc tactttcttc agtccgactt gagcattgat tggcgatgaa aggcttagga
     6541 gcaaaataca agtaatagtc aaataaatca gttttttcat gataattcct cccgccttca
     6601 tcgtaccacc agaaatttct gaaaagccgt ttcgccgttc ggtttctgaa acacaacgat
     6661 ataaacacca ctactgataa cctgttgact gagagtaatc atatcccaga aatagtaacc
     6721 ggcttccacc gggctatcat gtttgagaac ctgaaccagg tcgccatttt cggtaaagat
     6781 tttgattgta actttaccag gcaaattata gaactgaatg ccgcgctggt cagtccagcc
     6841 ctgagcggca agcaaaggat cgttgatatt gtaaggattg ggaacaatac ggattttagt
     6901 caaatcggtt tgcgaaaagt gaggtggatt gatccaatta atattcggca ccagcacccg
     6961 cgaactgtaa ataattttac cgcgagtcgt cgcatctgcc aaaggatcat cgtcggcaat
     7021 ccgggcttta gcctgcagat agtaataata ctgcgcgccg attaaaacat cgcgatcaat
     7081 atacaggtgg tcggtggctt tatcatcagg accggaatca taaatcacct gatagaaggt
     7141 agtatccatt gcgctcaggc ggcgcataat ccggtagccg gcaaagttcg gcatagcttc
     7201 ggcatccggg tcgcgccatt ttatttcgac cccggtgccc aaaccggtga tttcgagata
     7261 agttggcggc ggcggagcct gaggtatttt gtagccgtta tcaaagttcc attttgcacg
     7321 tcgggcgctt ttcataactg aatcaatgcc ggtactaacc cagcggtctt tgcgcaaatc
     7381 catttcggtc gcatctattg gaaaaacaaa attggatggt aaatagccgg tgtgagaatc
     7441 cggtagattg ggcgggtctt cgagagtgcc attaagccac tttttgccaa cttcctgagc
     7501 ggttggtaaa tccaagccag agtaaccgct ggcataaact atatgaattt tttgcccggg
     7561 cgcaaaagta taaggcccga aactgcagga catttttgaa ttgggactgc tcttcgtacc
     7621 agccggaaag tcggaataat cggaactacc attttcatcg ctgttgccac cgtgaaaggt
     7681 tccgggaatt gtatccccga tcatcggcca atatttggaa tagccacctc gaattgccca
     7741 gtaattctta ttgccgtatt catcactggc gtcgttataa ggaattttgg tcgcagtgcc
     7801 aatgtacgtt actttcggtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 ctggcgtcgt tataaggaat tttggtcgca gtgccaatgt acgttacttt cggttgggta
     7981 aaatcatcgc ggtcgttggt ccgatcatca tacggttcgc cggaagcgtg caggatcgaa
     8041 taccagcaaa actttgcccc gatcaaacgc ccgccctgcg cggtaactgg tgcgcccata
     8101 ttgtcgccgg caattgtcgg atcatcggcg ctgtattcat aaaatacccg cagagaatcc
     8161 ccgacccgac cgccataata atgttgccag actatagtag gattaaagcg ttcgttggaa
     8221 ggcggtgagg gattcgaacc attggaacga aaagcattga caccgttcga ttccatattg
     8281 ataaaaaagt cgtggagcgt atcctcgctg acattggtga aagtgacatc tacgatgata
     8341 tagtcgtcat tataattttg agtccaggct aaaatcttgc gatgaatatt gactcccaga
     8401 atgttctgag tggttacctc gacgatttgg tcgtaagttt cttcgccgaa tttacttggg
     8461 tcgtaggtcc caaacatttc taaattaatg ggattaaaat taattatttg ttcgggataa
     8521 gtataacgga cataattggt caatgggaca attactttgc cggtgcgcat ataatcgttt
     8581 gtgggtccat aaatagctac tttatgtatg gagtcgggat aaaccgggtc ctgccaattg
     8641 gtagccgcca gccgaaaacc ggcacccgcc caagcatcga gttcctgtcc ccgaatagcg
     8701 ataatatcgt aatcattagg gaaaaagccg gcccgatatt cgaaactacc cttgtcacca
     8761 ttggcggtca ccccgcacca aacgtgaccc attttgatta aagccgtggc gagttccggc
     8821 tgaatctggg aaaagccgaa ttgacccgcg cataatattg ccagcaagat caaggaaaaa
     8881 ttaatcctca ggttgttctt tctgtaattc ttcaagtcaa ttactccttt aaaaatcaat
     8941 ccttaaaaac taaacctcag accaaaccaa atatcgcgcg gttgttcgta gagccagaat
     9001 gaatagttgg gatcgttgat ataaggtttt tcttttgagc gcaattcgcc aatctggtca
     9061 ttgccggcaa tgtacattcc gggatattgc tgacgaagag cgtcatattt ggcggactcg
     9121 tacatcggga gacgaagcga gccaaaatat ttgacgaagt cactccattc ttccagatca
     9181 ccatcttctc tgacaaaaca atatccatca tccatcaagg tggtcttaaa attgaataaa
     9241 ttactgatat caacgtagaa agaggctgtt aatccgaaca agttaaactt cttatcaact
     9301 tttaaatcta ccatataata atcgggccag cgcatattgt tgttgacatg aagttcgttc
     9361 agcggattcc aggtaaaata ttcaccggct tgccattctg cgaagagagt aattgtccaa
     9421 tccgccagcg ggtaaatacc tgaaattttt gggccccatt tgtcgaatga tcgcacggtt
     9481 agattggcat taattttagg ttgtggcaaa ctgcgcgttt cctggccagc atatagtcct
     9541 tcctgatcgt tattgatggt aacgtcggta atcaggcttc gtccggtcaa gcctgtcttt
     9601 tttagcatat aattaaaatt gatccagccg gtcagccagc tggcatcact tttggaaaaa
     9661 ttaatttcaa ttccctgaat atcttgatat tcgttattag cccaagcatt gtaattgatc
     9721 gtgccgctgg cgttctggta ggttacttca ccatgttgtc cggtgacatc tttggaatac
     9781 caggacattt tcaaaacact cgtttgaaaa agtgtcatcg caattcccaa ttcgtaggaa
     9841 atcgtacggg gcggttccag attgggattc gacatatcat atagcccgtt tttagtataa
     9901 cgataacgat acaggtacat cgaatagtaa ggtggattgg agcggaaatg tccatagttg
     9961 aaataaaatt tcgattgttc cgtgactggg aaggaaatcc caatacgtgg actcaaagca
    10021 taatgatttt taatcggttg caggaagccg ggatggagtg aatcatatct aacccaagta
    10081 tcccagatgt aagactttcc tcttttcaaa taatcaaaaa gaaccgaatc ggacgcataa
    10141 ctctggggag taaaagcttc ggtagcaaat ggatcgccag taggccattt accgccgccg
    10201 ccgtaataat agtcaaagcg caaaccgata ttggcgatca tagattcaaa attcaattgg
    10261 tcttgaacgt aaatgccggt ctgggatgga gtgcgatggt aattgaattc gtaaacattg
    10321 taagcgtttt cgttccacat catccataga ttatgatcta aatctatata gttatactca
    10381 aagcccgtct tgacaaaatt gtggcgtccg atctgagaag atagatcgaa cttcgctcca
    10441 tactggccga ctcgcacatt attatacaaa tcgccctctt tgcggcggaa gcgataagcg
    10501 acgcctggca gagcgtcata attagggaat ttgtagctaa gagtatcgcc attgaatatt
    10561 ccggtgacgc gattcgtacc gtcgagtaat ttcccgtacg gcatttcatt gactacaaag
    10621 ggtccgaaat gggtaatggc tctcatatcg cgcgtgttgc cagtcggaga attgtcccga
    10681 atctgtaaag cgctgagttt gagttcccag aaagtcgttt tattgataat gtggttcacg
    10741 gttacgccgt tcactgcagt tctttgactc ataataggga aaaaaggtgg gtcataccaa
    10801 tattcgggat ttttatgaat atacttgatg ttattgaccg gcataaatcc accctggcga
    10861 ctggcatcgg gataatcacc ggaagccggc cgaatcgggc taaccccgtc ttcgtattta
    10921 taaaggccat tataggttag agtggtcttc ttagtcggtt ggcttttcat agttagtaaa
    10981 gtcgtatagg tctcgtcact gttgcgcgaa accggcataa caaagtggcg ctccttggca
    11041 ttaaatgata aataaaaagt agcgttaccc aatgccttgc cgaccagagg taccggaccg
    11101 ccaaaaccgc cgtcgaaatt ccaatcggga tattcttctc ggttgtgatg ctgagcgaag
    11161 agttttttct gctctttgga tacttcgtga tagccgatga cctgtttcac tgaatcggaa
    11221 agattagcca atcctttata atccggtatg gtcataaaca tccagcaagc cagcaggtaa
    11281 tagtcgaacg gagtcgccca ctggtcttcg gttttaccga tattatagtt gttggcttgc
    11341 gtgatccagc ccgtaaaact gggatactga tcacgtaagt aagcgtcgtc tttccaagcg
    11401 gtgtttgtcc caataaaagc cacctcagga tcaaggtaag agcgcaaagc cgcattatga
    11461 gcatcgtaaa atgaatcgcc gaaccggcgg atatgggcat tatcacgaga gtaactgaaa
    11521 gttccgtgat agtctttgga gcttcccgac ttggtcgtga tgttgatcaa tcccgaacgg
    11581 aaattgccgt attcggcatt ataaccaccc gaaagcaaag agacttgttc gatagcgctc
    11641 aacgggatac tgctttcggc attgccggtg gcggcattga catacgataa tccgtcaacc
    11701 atcattccgg tttgatcagc cgaaccgccc cgaatagtca gatagccatt ttgctcacca
    11761 attccgggta gtttagccag gaaggcgttg atttcccgaa taccggtagc atcgctgatc
    11821 tgcatcgcat caacaaccat ttgggtggtg gaaacttctt tgtgcagttc atcacgctta
    11881 gcagtaacaa taacttcttg tccctggatg acggtgcgat gcagttcgaa atcgaggcga
    11941 gtaatccgat cggcagaaac cataacattc gtaaatattt tggtctcgta acctatcatt
    12001 gaagcccgca cgcgatgagt gccgaccggt acgttaatga tgatataatt tccgtcggta
    12061 tctgtggcgg cgcccaaatc agtttcttca atgataatat taactccgat cagcggttca
    12121 ccggtctcgg cgtcgcgtac actgccgctg attcggctgc ggttctgccc ctgtaaatta
    12181 ctcgtgaatc cgactgtaag gattaaagcg gcgcagaaga aaaaaattat cggcttgttg
    12241 tttaacataa ctactcctaa agccgtgtgt tattttccaa cattattttt ttaacgaatg
    12301 atctgcattt tcaaaatttg gcgattacta gcaaattgca ttacgcagaa ataaattccg
    12361 gaagccactg gtttaccagc atcattgtcg ccttgccata ccaaccggta ttcgcccggc
    12421 atcagacttt gccgcggata agttttcaca acctgaccgg caaggtcata aatcctgatt
    12481 tcagccagcc cggttttgaa taatgaaaac tgtatggtcg tcgtcgcatt gaagggattg
    12541 ggataatttt gggcaagccg aaaatcagcg actacattcg gtttggaaac aatccgatta
    12601 ggattctgct cgaccagacc gttcaaataa ttttccaaac gggtatagcc attctcatct
    12661 agcaaatt
//
LOCUS       DAOMJU010000056        12584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5058_length_12584_cov_25.183414, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000056 DAOMJU010000000
VERSION     DAOMJU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5058_length_12584_cov_25.183414"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1004)
                     /locus_tag="PLO58_06415"
     CDS             complement(27..1004)
                     /locus_tag="PLO58_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQO74458.1"
                     /translation="MNLIDLQYAYGPVPSRRLGKSLGINNIPAKICTYACAYCQVGKT
                     DKMPVERQAFYKPEDIFRNVKYLLDNARHKGEQVDFLTFVPDGEPTLDINLGREISML
                     KTLGVPVGVITNSSLIWRTDVREEMNKADWVSLKIDTVDEAIWRRINRPNRSLDLQKI
                     LDGILQFARHYQGQLVTETMLIRDHNSSQAHIDRLVEFIAQLPDITAYLSIPIRPPSQ
                     KWAQAPTEEILIQAYYIFSSKIRNVKLIIEYEGNDFAFSGNVEADLLNITAVHPMREE
                     AIAKLLEKAKSDWSVVDSLIEKELLVKKEYRGKTFYTRRIKSLNDEIRK"
     gene            complement(1005..1652)
                     /locus_tag="PLO58_06420"
     CDS             complement(1005..1652)
                     /locus_tag="PLO58_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQO74459.1"
                     /translation="MSAFSENRNKQSKLLEKQKSFQSRTAQYLRLGYDRFAASDFVIA
                     AGGDLSSPALDIGTGKGLTAMALAKKGLDVISVDIETDEQAFALFLAREAGLEHRIKF
                     IGQNAAELPYPDNYFRCVVLSNILHHLENAAPVLEEAARVLAPRGIMIIADFSAEGFE
                     IVARLHREDGHLHSVSGETLKTAERFLNTKGFKTTNCLSGHMQEIIVLTQNDGIE"
     gene            complement(1658..2494)
                     /locus_tag="PLO58_06425"
     CDS             complement(1658..2494)
                     /locus_tag="PLO58_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQO74460.1"
                     /translation="MIIAVASGKGGTGKTTIAVNLAYALDREVQLLDCDVEEPNAQLF
                     LNGALLSTEEVTIPIPQIDASLCNGCGECSRFCEYNAIVSYGTMPLVFADMCHGCGGC
                     MKVCPPQAIREVDHRIGVIETFSAGKITLIQGKLDIGAAMAPPLIRAVKRKTDNRIPI
                     LLDAPPGTSCPVITTLRGADFVALVTEPTPFGLNDLILAVETVREMDLPFGVIINRSG
                     SGDARVNFYCADENIPILAEIPDDRKIAEAYSRGELIGKVFPEYRLIFEKIINDITTR
                     SD"
     gene            complement(2491..3366)
                     /locus_tag="PLO58_06430"
     CDS             complement(2491..3366)
                     /locus_tag="PLO58_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HQO74461.1"
                     /translation="MDLDCIPCFVQQAIRAARHLSDDPDVQERIVREALCCIGAVDFR
                     EPPPRASQIFYRRLSEITGIPDLYQADKDRQNRLALKLLPALKEQIGISPDPLLLAAR
                     LAIAGNIIDLGANGNLTIADIHEAVDQALNEQLVGDWEKFKSAVSEARDILYLADNAG
                     EIVFDRLLIEQILPKPVTLVVRGAPTINDALQTDAKAAGLDKLVDIIDNGSDAPGTLL
                     GDCSREFLRCFATADLIIAKGQGNYETLCNEPRNIFFLFKIKCPVIARHIGHPVGTQV
                     LLKSNEIQPENRSIH"
     gene            complement(3372..3854)
                     /gene="tsaA"
                     /locus_tag="PLO58_06435"
     CDS             complement(3372..3854)
                     /gene="tsaA"
                     /locus_tag="PLO58_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HQO74462.1"
                     /translation="MRIASVSFRPIGIIHSQHQNAEQIPIQPVYAKDCPAKVEVYQKF
                     AEGLRDLDGFSHIYLIYHFHRAKPAKLRVKPFLQDVERGVFATRSPNRPNAIGLSIVE
                     LIRREGNILHIKGADILDGTPLLDIKPYIKRFDRIDNSRDGWQEEIDEQTAQIRGRRK
                     "
     gene            complement(3851..4213)
                     /locus_tag="PLO58_06440"
     CDS             complement(3851..4213)
                     /locus_tag="PLO58_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HQO74463.1"
                     /translation="MKIAFTTSGNDLNAPLDNRFGRAEKFLIYDLENDTFEIIDNRQN
                     LNAAQGAGIQSAETIVRSGAKALVTAHCGPKAFRVLQSAGVKIYTTSAATVASALADY
                     RAGKLSESKSEDVAGHWI"
     gene            complement(4293..5006)
                     /locus_tag="PLO58_06445"
     CDS             complement(4293..5006)
                     /locus_tag="PLO58_06445"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQO74464.1"
                     /translation="MEPINETQSVSMPRIGDPAPAFKAITTQGEINFPQDYRGSWVIL
                     FSHPADFTPVCTSEFMTFATMEKQFADVNCKLVGLSVDGLYSHIAWLRTIKEKIDYKG
                     MKNVEVKFPLIEDITMEVAKKYGMIQPGESNTKAVRAVFVIDPKGIIRTIIYYPLSLG
                     RNFDELLRVVIALQTADAFNVSTPADWRPGDDVIVPTAGSCGVAKERMESKEGIKCLD
                     WFFCLKEITKEDVWKAITK"
     gene            complement(5122..5625)
                     /locus_tag="PLO58_06450"
     CDS             complement(5122..5625)
                     /locus_tag="PLO58_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQO74465.1"
                     /translation="MGTKAIEILNLDVKHLVDLLNRAYADEWLAYYQYWVGAKVVKGP
                     MKGAVIAELEEHAADELRHADMLVKRIIQLGGTPLIKPEDWYKHTNCGYDAPDDPHVK
                     EILEQNIKGEQCAITVYKKLFDLTAGKDPVTYNMVLEILEDEIEHEDDLQAELEDFEM
                     LVEKHSK"
     assembly_gap    5645..5744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5745..5944)
                     /locus_tag="PLO58_06455"
     CDS             complement(<5745..5944)
                     /locus_tag="PLO58_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HQO74466.1"
                     /translation="MPRGDGTGPARIGPMTGRAAGFCAGYNVPGYANPYVGRGFGGGM
                     GRGRGRGFRHWFYATGLPRWAR"
     gene            complement(5977..6570)
                     /locus_tag="PLO58_06460"
     CDS             complement(5977..6570)
                     /locus_tag="PLO58_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HQO74467.1"
                     /translation="MKVAFAIWNNRIAPVFDVARLLHIVEAENSVINRSVEEILPIDL
                     LPIKKAERLRELGVKTLICGAISRPLYDTIVANGIDVIPFIAGELDEVVQAWLSGRPD
                     WHHFAMPGCCGRRGRWQGGFHTNYQEMFIMSGKRRGMGSGQGRGGQRFGRMGGGSAAG
                     PSGNCVCPECGHKEPHQRGMPCFEQKCPQCGAAMARE"
     gene            complement(6758..8119)
                     /locus_tag="PLO58_06465"
     CDS             complement(6758..8119)
                     /locus_tag="PLO58_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HQO74468.1"
                     /translation="MTLQSALVENILESISDGVFTVDENWCITSFNRAAEEITGVKRK
                     EAIGQRCSEVFRSSLCGADCAMQKTLKTGKAVIGKSAYIIKADGARIPISISTALFKD
                     KNGKIIGGAETFRDLSEIETLRQELRNKMGFGDLVSRSPLMQKIFEVLPAIAASPSTV
                     LITGETGTGKELVARTIHQLSPRSRGPFIAVNCAALPNELLESELFGYKKGAFTGAVQ
                     DKPGRFALAKGGTLFFDEIGEVSPALQVKLLRVLQDRIYEPLGATRSETANVRIIVAT
                     NQDLTEMVKTGAFREDLYYRVNVVRIDLPPLRRRKEDIPVIIEQLVERFNLLWRKSVS
                     GVSIEALSLLMAHDWPGNIRELENVIERAFVLCNDGEITLKHLPEELTARRVTDLPPE
                     SIRSAVQTLEAQTIQSALARHNFNRTAAAKDLGIHKTTLFRRMKKLGLSLPRKDGRSH
                     PKK"
     gene            complement(8288..8431)
                     /locus_tag="PLO58_06470"
                     /pseudo
     CDS             complement(8288..8431)
                     /locus_tag="PLO58_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
     gene            complement(8441..8581)
                     /locus_tag="PLO58_06475"
     CDS             complement(8441..8581)
                     /locus_tag="PLO58_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74469.1"
                     /translation="MSNKIPFKARAISSQTAYFLAELASVASLSGQEEKVIARRMKLT
                     AP"
     gene            complement(8578..9426)
                     /locus_tag="PLO58_06480"
     CDS             complement(8578..9426)
                     /locus_tag="PLO58_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HQO74470.1"
                     /translation="MKFNIKSKIPTIICEWTIVFLFGAGNFLSAQVDTLVIPSVAMSK
                     NPKAVVVLPDVYHKSNDRFPVVYLLHGWSGSYRDWPTKMDLKPLADKYGFIIVCPDGG
                     YAGWYVNSPIVKDSQYETYIAKEVVDFIDANFRCVADSTGRFLCGLSMGGHGAFTLLA
                     KFPERFAGAGCMSGAMTMSSGERRAGLADVLGDWETNQQRWEANSALFLAERLVGHDK
                     AILIDCGVDDFLHQTNFLLHKKLLDLKIPHVYIERPGAHTWDYWTNALEYHLLFFRNN
                     FYKDAK"
     gene            complement(9728..12478)
                     /gene="ppdK"
                     /locus_tag="PLO58_06485"
     CDS             complement(9728..12478)
                     /gene="ppdK"
                     /locus_tag="PLO58_06485"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQO74471.1"
                     /translation="MTTQKSKTPKYVYFFGGGKADGNESMKELLGGKGANLAEMAGHP
                     DLRLPVPPGFTITTEVCTYYYQHNRKYPAELKEQVEAALAKVEKIMGRKFGDPDNPLL
                     VSVRSGARKSMPGMMETVLNIGLTEKTIPGLIKQSGGNERFVYDAYRRLIMMYSDVVM
                     EKAAGIEPKDDEGIRRQLEKIMDDFKKAKNYTSDTDLTADDLKELCRVFKEQVKKVLG
                     KDFPDDPYEQLWGGIGAVFASWNGKRAVAYRRIEGIPDDWGTAVTVQAMVFGNMGDDC
                     ATGVAFSRNPGNGENQFYGEYLINAQGEDVVAGIRTPAPINKYSASEHNKDLVTLEEY
                     MPAIYKELVEYRNRLEKHYRDMQDIEFTIEKGRLFMLQCRIGKRNGPAAVRMALDMLN
                     EKLITKEEAVLRVSPSQLDELLHPIIDPKHEKKIEPLAKGLPAGPGAATGQIVFSSAD
                     AVEWAKAGKKVILVREETNPEDVDGMRASQAILTARGGMTSHAALVARGWGKCCIVGC
                     SAIHIDYQKKVMKIGDRVFKEGDFITLNGTKGYVYAGTLPMIDATENPDLVKFLKVCD
                     SVRRLGIRTNADTPADAKRARQFGAEGIGLFRTEHMFYGEGSDAPLFALRKMIVSNTL
                     DERQKALDELFPYVKKDIKATLEAMDGYPVVIRLLDPPLHEFVPREKEKQEKLARDLN
                     ISLDELEKRAAELHESNPMMGHRGVRLGVTYPEVSRMQIRAILEAAAELLKEGKKPYP
                     EIMIPVVSLTKELEHQKAIVDEVYQEVLKKHGLKEIKYLFGTMIEIPRACLVANEIAG
                     VTDFFSFGTNDLTQMGFGFSRDDIGSFVPDYIEKKILPEDPFQTIDQVGIGELIQIGI
                     ERGRKTKPNLEIGICGEHGGEPRSVKFCHRVGMDYVSCSPFRVPIARLAAAQAVAEEK
                     TK"
BASE COUNT         3198 a         3119 c         2675 g         3492 t
ORIGIN      
        1 gcattttccg tctccggcaa ccttgttcac ttgcggatct catcgtttaa agattttatg
       61 cgtcgggtat aaaatgtttt cccccgatat tcttttttta ccaaaagctc tttttcaatt
      121 aaactatcaa cgaccgacca gtcagattta gctttttcca ataatttggc aatagcctct
      181 tcgcgcatcg gatggacagc ggtaatattc aacaaatccg cttccacatt gccgctaaaa
      241 gcaaaatcgt ttccttcata ttcaattatt aatttcacat tccggatttt agagctgaaa
      301 atataataag cctgaatcag tatttcttcg gttggagctt gggcccattt ttgggaaggc
      361 gggcgaatag gaatggaaag ataggcggtg atatccggca actgggcgat aaattccacc
      421 agccggtcga tatgcgcctg actgctgtta tgatcgcgga tcagcatcgt ttcggtaaca
      481 agttgacctt gataatgcct ggcgaattgc agtatgccat cgagaatttt ctgaagatca
      541 agcgaccggt tcggacgatt aatcctgcgc cagatcgctt catcaaccgt atcaattttc
      601 agcgaaaccc agtcagcttt attcatttct tctcttacgt ctgtacgcca gatcagcgaa
      661 ctgttggtaa tcacaccgac cggaactccc aaggttttca acatactgat ctcccgacca
      721 aggttaatgt ccagcgtagg ttcgccatcc ggaacaaaag tcaggaaatc aacctgttcg
      781 cctttgtgcc gggcattgtc caaaagatat ttcacgttcc tgaaaatgtc ttccggtttg
      841 taaaaagcct gacgttcaac cggcattttg tcggtttttc cgacctgaca gtaagcgcag
      901 gcataagtgc atattttggc ggggatattg ttgattccga ggcttttccc cagccgtcgc
      961 gatgggacgg gaccgtaggc atattgtaaa tcgataaggt tcaattattc tataccatca
     1021 ttttgagtca aaacgataat ttcttgcatg tgaccgctta ggcagttagt tgttttaaat
     1081 ccttttgtat ttaagaatct ttccgccgtt ttcagtgtct ctccgctaac cgaatgcaga
     1141 tgaccatctt cgcgatgtaa gcgcgcaacg atttcaaaac cttctgcaga aaaatccgct
     1201 atgatcataa tccctcgcgg cgccagaact cgggcggctt cttccaatac cggcgcggca
     1261 ttctccagat gatgcagaat atttgaaaga acaacacatc taaaataatt atcgggataa
     1321 ggcaattcgg cggcattttg gccgataaat ttgattcgat gctcaaggcc ggcttctctg
     1381 gctaaaaaca gcgcaaaggc ttgttcatcg gtctcaatat caactgaaat tacatccaat
     1441 ccttttttcg ccagcgccat agccgtcaac cccttgccgg taccgatatc cagcgccgga
     1501 ctggaaagat cgccacccgc ggcgatcacg aaatcggaag ccgcaaagcg atcatagccc
     1561 aaacgcaggt attgggcggt gcgggactgg aaagatttct gtttttcaag caatttgctc
     1621 tgtttatttc tattttctga aaaagcgctc atatatttta atcacttcgg gttgtaatgt
     1681 catttataat tttttcaaat attaggcgat attcgggaaa aacctttcct atcagttcgc
     1741 cgcgcgaata ggcttcggca atctttcgat catccggaat ctccgccaga atcgggatat
     1801 tttcatcggc gcagtaaaaa tttacccgcg catcgccact tccggaacgg ttaataatga
     1861 ccccgaaggg tagatccatt tcgcggacgg tttcaaccgc cagaatcaga tcatttaatc
     1921 caaaaggtgt cggctcggtc acaagcgcga caaaatcggc tccccggagc gtcgtaatta
     1981 ccgggcagga agttcccggc ggcgcatcca ataatatcgg aatacgattg tccgtttttc
     2041 gtttcacagc ccgaattaac ggcggagcca ttgccgcgcc aatatccagt ttaccctgga
     2101 tcagagtaat tttcccggca ctaaatgtct caatgacgcc aatacgatgg tcgacttcac
     2161 ggatcgcttg gggcggacag actttcatac acccgccgca tccatgacac atatctgcaa
     2221 atacaagcgg cattgtacca taggatacaa tggcattata ttcgcaaaaa cgactacatt
     2281 cgccgcaacc attacaaaga cttgcatcga tctgaggaat cggaattgtc acttcttcag
     2341 tagagagcag agcaccattc agaaacaatt gggcattcgg ttcctcgacg tcacaatcca
     2401 gaagctgtac ttcacggtcc agggcatagg ccaggttgac ggcaatggtc gtcttgccgg
     2461 ttccaccctt accggacgca accgcgatta tcaatgaatg ctcctgtttt ctggctgaat
     2521 ttcattggat tttagcaaaa cctgggttcc gaccggatga ccaatatgcc tggcgattac
     2581 cggacatttt attttgaaca ggaaaaaaat attgcgcggt tcgttgcaaa gagtctcata
     2641 attgccttga cccttggcaa tgatcagatc ggcagtcgca aagcacctta gaaattcacg
     2701 actgcaatca ccaagcagag ttccaggcgc atctgagccg ttatcaataa tatcgacaag
     2761 tttatctaga ccggcggctt ttgcgtcagt ttgcaaagca tcgttgatag ttggagcgcc
     2821 acgcactacc agtgtcaccg gtttaggtaa aatctgctcg atcagcagac gatcaaaaac
     2881 tatttcaccg gcattgtcgg ccaagtatag aatatcgcgg gcttcgctaa ccgccgattt
     2941 aaatttttcc caatcgccga cgagctgttc attcagagcc tgatcgactg cctcgtgaat
     3001 atccgcgatt gtcagattgc cgtttgcgcc gagatctata atatttccgg caattgccag
     3061 gcgcgccgcc agcaaaagcg gatcgggcga aatgccgatc tgttccttta acgctggcag
     3121 taatttgagt gcaagccggt tctgtctatc cttatcagcc tggtataaat ccggaatgcc
     3181 ggtaatttca ctcaggcgac ggtaaaatat ttgactggcg cgcggtggcg gctcgcgaaa
     3241 atcgacggca ccgatacagc agagagcttc tcttacaatt cgttcttgaa catctggatc
     3301 atcagataaa tgccgcgcgg cccggatagc ctgctgtaca aaacaaggaa tacagtcgag
     3361 atccatttgc attatttccg gcgccctcta atctgagcgg tttgttcatc gatctcttcc
     3421 tgccagccgt cacgactatt atcgatccgg tcaaagcgct ttatatacgg tttaatgtcg
     3481 agcaaaggag taccgtccaa aatatctgct cctttgatat gcaaaatatt gccctcgcgt
     3541 ctgatcagct cgacaatgct caatccgatc gcgttaggtc ggttgggaga ccgtgtcgca
     3601 aatacgccgc gctcgacgtc ttgcagaaaa ggcttcaccc gcagtttagc cggtttagcc
     3661 cgatgaaaat gatatatcag gtaaatatgc gagaagccat ccagatcacg cagtccttcg
     3721 gcaaatttct gatagacttc aacttttgcc gggcaatctt tggcgtaaac cggctgaatt
     3781 ggtatctgtt ccgcgttttg atgctggctg tgaattatcc cgatcggtcg gaatgaaact
     3841 gaggcaattc tcatatccaa tgtccggcga catcttcgct cttagattcg ctaagcttgc
     3901 ctgcccggta atccgccagg gcggacgcga ccgttgccgc acttgtggta taaatcttga
     3961 caccggcgga ttgcaaaacc cgaaaagcct ttggcccaca atgggcggtg accagcgctt
     4021 tggcgccgct tcggactatg gtttccgctg attgaatccc agcaccctga gcggcattca
     4081 ggttttgccg gttatcgatt atctcgaatg tatcattttc aaggtcataa atcaggaact
     4141 tctctgctcg cccgaaacga ttatcgagtg gtgcatttag gtcattgcct gaagtcgtaa
     4201 atgcaatttt cataatacac accccttaat tcaaggttgc gtgagcctga taacccacgc
     4261 aacctcgcag ttttaatttc cggaatcgcc aattacttgg taatggcttt ccagacgtct
     4321 tccttggtga tctccttcag acagaagaac caatcgagac acttaatgcc ctctttgctc
     4381 tccatacgtt ctttggcaac gccgcaggag ccagcggtcg gaacgatgac atcatcacca
     4441 ggacgccaat cagccggagt tgaaacgttg aaagcatcag ctgtctgcag agcaattact
     4501 acccgcagca attcgtcgaa attacgtccc agacttaacg ggtaataaat gattgtgcgg
     4561 ataatgcctt taggatcgat tacgaagacg gcgcgcacgg ctttagtatt gctttcaccc
     4621 ggttgaatca tgccgtattt cttggcaact tccatcgtta tgtcttcgat caatggaaat
     4681 ttgacctcga catttttcat acctttgtag tcaatttttt ccttaatggt gcggagccag
     4741 gcgatgtggc tgtatagacc gtcaaccgaa agtccaacga gtttgcagtt cacatcggcg
     4801 aattgctttt ccatagtggc aaaggtcata aattccgaag tgcagaccgg cgtgaaatca
     4861 gccgggtggc taaataatat tacccagctt cccctataat cctgcgggaa attaatttca
     4921 ccttgagtag ttatagcttt gaaagccgga gccggatcgc cgatacgggg cattgatacg
     4981 ctctgagtct cattaatagg ttccatgttt ttttcctttt atggtttgtt tatatcgctt
     5041 tttaaataag tcggtctgaa taccctaaaa aacataccgg atcaacatat ttgctaaaca
     5101 aacggtatgt ttctaactgg cttatttaga atgcttttct actaacattt caaaatcttc
     5161 gagttcggct tggagatcgt cttcatgttc gatctcatct tccagaatct ccagaaccat
     5221 attataggtg accggatctt taccagcggt aaggtcgaat aattttttat aaaccgttat
     5281 agcgcactgt tcgcccttga tattctgttc aaggatttct ttaacatgcg gatcatccgg
     5341 tgcatcgtaa ccacaattgg tgtgtttgta ccaatcctcg ggtttgatca atggagtccc
     5401 acccagctgg attatacgtt tcacgagcat gtcggcatgg cgcaattcat cagccgcgtg
     5461 ctcttccagc tcggcgataa cggcaccctt catcggtcct ttgacaactt tcgcgccgac
     5521 ccagtactga taataagcca accattcatc ggcatatgcc cgattcagca ggtcgacaag
     5581 atgcttcaca tccaaattaa gaatttcaat tgcttttgta cccataattt tctcctttta
     5641 ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaatctt gcccacctcg
     5761 gcaatccggt cgcataaaac caatgtcgga atccccgacc tcggccgcgt cccattccgc
     5821 cgccgaaacc gcggccgaca tacggattag cgtaacccgg cacattgtat cctgcacaga
     5881 atccagccgc gcgtccagtc attggtccta ttctggcggg accagttcca tcacctcttg
     5941 gcatagtttt tttctcctta tttttattgt tgtcatttat tctctcgcca tagccgcacc
     6001 acactgtgga cacttttgtt caaaacaggg cattccacgt tgatgcggct ctttgtgccc
     6061 acattccgga caaacacaat taccacttgg accagcggct gaacctccgc ccatacgacc
     6121 aaagcgctga ccaccacgcc cttgaccact tcccattcca cgtcttttcc cactcataat
     6181 aaacatctcc tggtaattag tatgaaaacc accttgccac cgacctcttc taccacaaca
     6241 acccggcatg gcaaaatgat gccaatcagg gcgtccgctg agccaggctt gaacaacttc
     6301 atccagctcg ccagctataa atggaatcac atcaataccg ttagctacaa tcgtatcata
     6361 taacggcctg gaaatggcac cgcaaatcag tgtcttaacg cccagctctc tcaagcgttc
     6421 tgctttttta attggcaata gatcaatcgg taaaatttct tcaaccgatc tatttatcac
     6481 actgttttcg gcttctacga tatgcagtaa tcttgccacg tcaaaaacgg gtgcgatgcg
     6541 attattccag atagcaaagg cgactttcat tattttcttt tccaactaaa catttcacta
     6601 atgcgaggtg catttttcgt gccacaatcc ggatactgta attattccac tacaacaaca
     6661 ataaatacaa tatgtaaaga atttcttgtg ctgggctaac tgtactcgca cagtagccat
     6721 attgcaactc aatcgtaata gtacagtagc gtatacgcta ctttttaggg tgcgatcgac
     6781 cgtcttttct tggtaaagaa agaccgagct tcttcatgcg gcggaacagg gtggttttat
     6841 gaataccgag atccttagca gcggcggtgc gattaaaatt atgacgggct aacgcagatt
     6901 ggatggtttg agcctcaagg gtctggactg cggagcggat actttcaggt ggcaaatcag
     6961 tgacgcggcg ggcggtcagt tcttccggca gatgcttcag tgtaatttcg ccatcattgc
     7021 agagcacaaa agcgcgttcg atgacgtttt ccagttcacg aatgtttccc ggccagtcgt
     7081 gcgccatcaa taacgacaat gcttctatac taactccact tacggattta cgccaaagca
     7141 gattgaaacg ctcaaccagt tgttctataa tcacggggat atcctctttt cggcgacgca
     7201 ggggcggcag gtcaatccgc acaacattta cgcgatagta gaggtcttcg cggaaggcgc
     7261 ctgtcttgac catttcggtt aaatcctgat tggtagctac tatgatcctg acattagcag
     7321 tttcagagcg ggtagcgccg agcggttcat aaattcgatc ctgcagaacg cgcagaagtt
     7381 tgacctgtag cgccggactg acttccccga tttcatcgaa gaacaacgtg ccgcctttgg
     7441 ctaaggcaaa gcgtccgggt ttatcttgaa cagcgccggt aaatgcacct tttttataac
     7501 caaataattc cgactccaac aactcgttcg gcagagcggc acaattgaca gctataaaag
     7561 gccctcggga gcgtggactt aattggtgaa tagtgcgggc aaccaattcc ttgccagtac
     7621 cggtctcacc ggttattaac acggtgctag ggctggcggc gatggcgggc aatacctcga
     7681 atattttttg cattagtgga ctacgactga ccaaatctcc gaaacccatt ttgttgcgca
     7741 gttcttgtcg taaggtttcg atttcactaa gatcccgaaa ggtttcggcg ccaccgataa
     7801 ttttgccgtt cttatcttta aacagtgccg tggaaatgct gattggaatg cgggctccgt
     7861 ccgccttgat aatataggcg gatttcccaa tcacagcttt accggttttc agagtttttt
     7921 gcatggcgca atcggcgccg cacaaacttg atcgaaacac ctccgaacag cgttgaccga
     7981 ttgcctcctt acgcttcaca cccgtaattt cttccgccgc acgattgaat gaggtaatgc
     8041 accagttctc atccaccgtg aaaacgccat cagagatgct ttccaggata ttttcgacca
     8101 atgcggattg aagtgtcatt tcatatcaaa gttaagaaat attttaacac agaacactca
     8161 gagaacgcga cggttattta aacaaatcct ctcgccttca gagcgttgct tttgttattt
     8221 tcacaaattg ttttcaattt ttattaaaac tcgatgagct ctgcactctc tgtgacgagt
     8281 ttaaacttta cggtctgcca cccattgtca acgccatcac agctttagcg gcgtgcaggc
     8341 ggttttcggc ttcgtcataa attatcgccg ccggtccatc aataacctcg tcggtgacct
     8401 tcttgccgcg gtcggctggc agggcatgca tttattttac ttatggggcg gttaatttca
     8461 tgcggcgggc gatgaccttt tcttcttggc cgctcagcga agctacggaa gccagttctg
     8521 ccagaaagta ggcggtttgg gaactgattg ctcgggcttt gaatggaatt ttattgctca
     8581 cttcgcatcc ttataaaaat tatttctgaa gaatagcaga tgatattcga gagcatttgt
     8641 ccagtaatcc caagtatgag cgccggggcg ttcgatatat acatgtggaa tcttcaggtc
     8701 tagtaatttt ttatgtagaa gaaaattagt ttgatgcaaa aaatcatcaa ctccgcaatc
     8761 aattaaaatt gccttatcgt gtccgaccaa gcgctcggct aaaaataaag ccgaattcgc
     8821 ttcccacctt tgctggttcg tttcccaatc accgaggaca tcggccagcc ctgcccggcg
     8881 ctcgccgctg gacatcgtca ttgcgccgct catacagcct gccccggcaa aacgttccgg
     8941 aaatttagcc agtaaggtga aagcaccgtg tcctcccata ctcagtccac aaagaaaacg
     9001 cccggtggaa tcagctacgc atcggaaatt tgcatcaata aaatcaacta cctctttagc
     9061 aatgtaagtc tcatactgtg agtctttcac aatcgggcta ttcacatacc aaccggcata
     9121 gccgccatcc gggcagacaa taatgaaacc gtacttgtcg gctaacggct tcaaatccat
     9181 tttagtcggc caatcgcgat atgagccact ccagccatgt aacaaatata caactggaaa
     9241 acgatcattc gatttgtggt atacatctgg caaaacgacc acggctttag ggttcttaga
     9301 catagccaca ctcggtatta ccaaagtatc cacctgggca gataaaaagt taccagcccc
     9361 gaataggaag acaattgtcc attcgcagat aatcgttggt attttgctct ttatgttaaa
     9421 tttcattatt acctctctgt ttgattatca aatatcttta ttatttcgga agtctccaat
     9481 agttttcatc gaatttcagg attgagctca cgaccaattg gctgactttc ctggtctaaa
     9541 ataacttcgg atggtcagct gtaaaagata tgttggtcaa aataataaaa attatttccc
     9601 tcttaattac gattccgtcg cattcagcaa tgagaaaacg ccaaaaataa gaagaacggc
     9661 tctacttaaa aaaagaaccg cagaccaaaa tctgcggctc tttatttatc tatattgaaa
     9721 aaacgtttta cttcgttttt tcttcggcaa cggcttgggc ggctgccagt cgagcaatcg
     9781 gcactctgaa cggtgagcag gaaacataat ccattccaac tcgatgacaa aacttgacgg
     9841 atctcggttc gccaccatgt tcaccacaaa taccgatttc caggttcggc ttggtcttgc
     9901 gaccacgttc gataccaatt tggattaatt cgccaatgcc gacctgatct atggtctgaa
     9961 aaggatcttc cggtaggatt ttcttctcga tataatccgg cacaaaactg ccaatatcat
    10021 cacgcgaaaa tccaaatccc atctgagtaa gatcgttagt cccgaatgag aagaagtcgg
    10081 tgaccccggc gatttcattg gcaactaagc aggcacgtgg aatttcgatc attgtaccga
    10141 ataaatattt gatttctttc aggccgtgct tcttgagtac ttcctgataa acttcatcta
    10201 caattgcctt ttggtgctcg agctcttttg ttaaactgac cactggaatc attatttccg
    10261 gataaggttt cttgccttct ttcaataatt cggcggcagc ttccaagatt gctctaatct
    10321 gcattcggga aacttctgga taggtcactc ccagtcgaac accgcgatgt cccatcattg
    10381 ggttagactc atgcagttca gcggcacgct tttccagttc atctaaggaa atattcaaat
    10441 ctctggcaag tttttcctgc ttttcttttt ctcgggggac aaattcatga agcggcggat
    10501 cgagcaaacg gataactacc gggtagccgt ccatcgcttc cagggtcgcc tttatgtcct
    10561 ttttaacata gggaaagagt tcatcaagag ccttttggcg ctcatctaat gtattagata
    10621 caatcatttt tcgcagagca aagagtggcg catcgctacc ttcgccgtag aacatatgtt
    10681 ccgtacgaaa tagcccgatt ccttctgccc cgaattgacg cgcgcgttta gcatcggctg
    10741 gagtatcggc attcgtacgg ataccgagac gacggacaga gtcacagact ttaagaaatt
    10801 ttaccagatc aggattttcg gtagcgtcaa tcatcggtag agttccggca tatacataac
    10861 ccttagtccc attcagagta ataaaatcgc cttccttaaa aacgcgatca ccgattttca
    10921 ttactttttt ctgataatct atatgaatag cactgcaacc gacgatacaa catttgcccc
    10981 atccgcgagc aacaagcgcc gcatggctgg tcataccacc gcgagcagtt aaaattgcct
    11041 gtgaggcgcg cataccgtcc acatcttcag gattggtttc ctcgcgtacc aaaattactt
    11101 ttttgcctgc tttggcccat tcgacggcat cggctgaaga gaatacaatt tgaccagtgg
    11161 cggcaccggg accagccggc aaacctttgg caagcggttc tatttttttc tcgtgtttcg
    11221 gatcaataat agggtgcagt aattcatcga gttgagaagg cgaaactcgc aagaccgctt
    11281 cttctttggt gatgagtttt tcattcaaca tatctaatgc catccgtacg gcggcgggac
    11341 cattacgctt accgatgcgg cattggagca taaaaaggcg ccccttttca atggtaaatt
    11401 cgatgtcctg catatcgcgg tagtgttttt cgaggcgatt ccggtattct acgagctctt
    11461 tataaatagc cggcatgtac tcttccagag tcacaaggtc tttattatgt tcagaagccg
    11521 aatatttatt tattggtgct ggggtgcgaa tgccggctac tacatcttcg ccctgagcat
    11581 ttattaaata ttctccataa aattggtttt ctccatttcc ggggttacgg ctaaaagcta
    11641 cgcctgtagc acagtcgtcg cccatattac caaagaccat tgcctggaca gtcacagcgg
    11701 tgccccaatc atccggaatg ccttcaatac gacgataggc aaccgcccgt ttgccgttcc
    11761 aggaagcaaa cacggctccg ataccacccc ataactgttc atacggatcg tccggaaaat
    11821 ctttaccaag cactttctta acctgttctt taaacacccg acatagctct ttcagatcat
    11881 cggcggtaag atcagtgtcg ctagtatagt tttttgcctt tttgaaatcg tccataattt
    11941 tttctagctg acgacgaatt ccctcatcgt ctttcggctc gatcccggcg gctttttcca
    12001 taactacatc ggaatacatc ataatcaaac ggcgataggc atcgtaaacg aagcgttcat
    12061 tgccgccgct ttgcttgatt aagccgggta tggttttttc ggttaaacca atgttcaaaa
    12121 cggtttccat catgccgggc atagatttgc gagcgccgga gcggactgat accagtaagg
    12181 gattatctgg gtcaccgaat ttacggccca tgattttttc cactttggct agagccgctt
    12241 cgacttgctc cttcagttcc gccggatact tgcgattatg ttgataataa taggtgcaga
    12301 cttcggttgt gattgtaaaa ccgggcggca ccggcaagcg cagatcggga tgaccagcca
    12361 tttcagccaa gttagcgcct tttcctccca acaattcctt catactttca tttccatcgg
    12421 ctttaccacc gccgaaaaag tagacatatt taggggtttt agatttttga gttgtcattt
    12481 ttttacctct tcatgttatt ctattaactg taaaaaacca gcgctaagtt tacgccgaat
    12541 tgactttttt ttcaagtttt agttataccg agtagattaa tctt
//
LOCUS       DAOMJU010000057        12494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5104_length_12494_cov_21.069218, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000057 DAOMJU010000000
VERSION     DAOMJU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5104_length_12494_cov_21.069218"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..346)
                     /locus_tag="PLO58_06490"
     CDS             complement(170..346)
                     /locus_tag="PLO58_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74472.1"
                     /translation="MKGFGAVLVAISLILLVLEHLNLVDVVGIIGLLVGLYAIIFPDK
                     AEELYTKLKSGKLK"
     gene            737..1507
                     /locus_tag="PLO58_06495"
     CDS             737..1507
                     /locus_tag="PLO58_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74473.1"
                     /translation="MNRKQFFPFVFFMVLAGSLSAQGDLGIHQPVNLVTIPTAGILPR
                     GSYAVDCHLFPGGGLAGKISVGVFERFMFGVSYGGGGIIGNQKINWYNQPGVEIKYRF
                     IEESVKFPALLVGFSSQGHGNYIDSLKRYETKASGFYVVVSKNFNFLGNLGLHGGINY
                     NPIEKTDGDADPNFFLGIDKDLNSEIALIIEYDAALNDNHTELPLLGKGWGYLNAGVR
                     WRLVDKFTVELDFNNILLNRKDVDFMNRELKFTFTEIF"
     gene            1589..2374
                     /locus_tag="PLO58_06500"
     CDS             1589..2374
                     /locus_tag="PLO58_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74474.1"
                     /translation="MRKLFFSNSTRVFFIVLGLCVLPVYLTPQSTKVKSMLEQADKLH
                     DARDYQGTMTKLREAEKIEPNNPEVLWRLARAYFDIADQQPGVSEVQKANLYPGFEYA
                     KKCVELAPEVAGGHQYYAILIGQIGELEGTKQKITNSYAVREHTEKAIALDPKEDSNY
                     HVMGRWHFELADLSWAERQVASLIYATPPKASFEEAEQYFQKAHELKPKEIRHLLWLG
                     KTQLKLKKKDQAKKNLQLALEIPAQSDSDKVMQKEAADLLKKL"
     gene            2390..2524
                     /locus_tag="PLO58_06505"
     CDS             2390..2524
                     /locus_tag="PLO58_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74475.1"
                     /translation="MKYLKLIFKILAVITVIGLLILAVLLIIQKKSKSESVAEEPATE
                     "
     gene            complement(2653..3291)
                     /locus_tag="PLO58_06510"
     CDS             complement(2653..3291)
                     /locus_tag="PLO58_06510"
                     /inference="COORDINATES: protein motif:HMM:NF027727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HQO74476.1"
                     /translation="MALTQNLQLHYDFGRQHLTSTYELYKTDRLGATFLFVDFDYNNE
                     GSSGSRNASLAYGEIARYFQLPKMNDLSATLQYNDGLTNSGSFNSVWLGGLQYVFQIG
                     TQNFPIDFLLRKELNTDGLTFQLTYVWSYIWRKLELSGFIDIWNTGADAYPPRKIVIL
                     SEPQFWYRLTPALSVGGEIEISFNFSGAWHTNQVFSEDVIFILPTLGLKWLF"
     gene            complement(3329..3760)
                     /locus_tag="PLO58_06515"
     CDS             complement(3329..3760)
                     /locus_tag="PLO58_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74477.1"
                     /translation="MTSSSRILYSYIILGIALVAFGIATILGHFYIGLICLMLGMIIW
                     LIVTFAGNTEGQSGQIGVVMITAGFIGGLIIFIGIGLRPSIFGGHKLYIEGIVTALVV
                     LLIFMAIGLGFISLANLEKKMAFFNKELKKFYKLYMKEDNE"
     gene            complement(3747..5225)
                     /locus_tag="PLO58_06520"
     CDS             complement(3747..5225)
                     /locus_tag="PLO58_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HQO74478.1"
                     /translation="MVINLWAELIAEVPQVVIVHWNDFHSTNLPYKSSQADYRVGGYA
                     NLAGYFDSLRHLHPQAIILNAGDDFQGSPVSNLTRGLSQILILNKVQPTAFTLGNHEF
                     DYGIDNLRNVMTYANFPILNSNLFDSTKNDLLVQPYTIIQSGNLKVAIIGALTGDLFT
                     LALPQNLKGIGILDPLTQIRQYVNEVEPQTDLIVLLSHCGFEEDSLLAMQLAGVDVII
                     GGHSHTYLHQPKKVNNILICQAGSNGHCLGYLKANIDRNQKIITSYNYELIETRLGRV
                     KPSPIVAGVVDSLEASISAEMDKIIGQLITDWHRNSHGESNLGNWICDVTREYFKTDI
                     AFMNSGGIRKDLKAGPIRVRDLWEISPFDNTIVIVALTGEQLKFIIQYHVENPRDLLQ
                     VSGLSYEFNSKQKKLVKLTVAGKPVDDQKIYTFATNNFVISQFERFFGLAPSAVKITP
                     TDIIGRDILIEAIKAQPVINNHTEGRIIDLANKKEEVNYDIQ"
     gene            5392..6384
                     /locus_tag="PLO58_06525"
     CDS             5392..6384
                     /locus_tag="PLO58_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQO74479.1"
                     /translation="MLKIGVVGAGNFGRWHIKNLQNLPNAELVGFFDNNPERASYISN
                     EFQVRAYPALEQLLDDCEAVSIVVPTHSHYSVAKAAIDCGRHLLCEKPFMETVAEAEE
                     IIRLARAKNLVLQVGHVERFNPALGALKQYQIDPLFIESHRIAPFRGRGTDVAVVLEL
                     MIHDIDLVLDLVTAPIVQVEASGAPVLTDNIDIANARLCFANGCVANVTASRVSNKQM
                     RKLRIFQRDAYFSIDMLAGSTEIYSLSRDAGPVPDDTTQIMEVPDVNGAPRRIFYRVQ
                     QTEQGNALYNELESFVNAILTGQPPRVSGEDGIRALKVAIQIINSIQDNFRKIN"
     gene            6384..6968
                     /locus_tag="PLO58_06530"
     CDS             6384..6968
                     /locus_tag="PLO58_06530"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQO74480.1"
                     /translation="MDIRRLFFKIRGYTPIPLMLVLIYQSDMNFKFSIIGAILALVGE
                     YIRLNGVRAAGGRTRTRNVGAKELCTWGIFAHTRNPLYIGNALIYIGMVFFAGGRWLL
                     PLMLLVFVYILFQYGLIISLEEETLTNIFGAEYAKYKANVPRLWPRLKPWNSGVQPKF
                     LSNRKLLRTERSTLSVFVIFALAVILKEWLNVRW"
     gene            6955..8079
                     /gene="lpxB"
                     /locus_tag="PLO58_06535"
     CDS             6955..8079
                     /gene="lpxB"
                     /locus_tag="PLO58_06535"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQO74481.1"
                     /translation="MSAGKLFISAGELSGDMHAGKLISALKELNPDIEISAIGGDNMA
                     AAGAELLFHIRETSFMGFVEVIKHLPFIISMYRKTLQFLDEFKPDVVVLIDYPGFNLR
                     LAKALDRRDIPVIYYIVPQVWAWHQSRVKKIRRHVQEVICILPFEATWFSERGVRATF
                     VGHPLLDQNEISIPEPNQKIFKNGEPLVGLFPGSRQQEVDKHLPLMISSVKDLRQEFP
                     NLQAAVAVASDVNLEKYQKCYQFDWLSWHFNSTRSVIQSADVLIMASGTVTLEATLSN
                     KPFVVIYRVAPLTYWLGKKLIKVPFISITNLIAGKKGVTELIQHDANVENIKSEIMRI
                     LCDQSYANQIRDFLQYVCRLLGEPGASRRAAHIILNYLES"
     gene            8095..9150
                     /gene="lpxK"
                     /locus_tag="PLO58_06540"
     CDS             8095..9150
                     /gene="lpxK"
                     /locus_tag="PLO58_06540"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQO74482.1"
                     /translation="MQLGALRWLLFPAAVIYGLITKLRNLFYDRHIFKAIKVPVPVVA
                     VGNITAGGTGKTPFVIALAKFLSEKGYRIAIISRGYRRKSRGTVIVSDGRQILTTPQQ
                     AGDEPFLMAGKLPGVVIVADADRIAAAHTAIENFACNLIIADDAFQHRRLARDINIVL
                     WDPKTPSWQKYLFPVGRLRESWHGLKRADYLVFSKTDKIIPEQRTFFEKFNPVISFFT
                     APLKIVGLRTLSAENVSINTIAGKNVLAFCGLGNHIQFETTTRQLNCGKVTFLRFPDH
                     HHYTTTDLQKIIIKAEQMNCDYLVTTEKDAVNLPVNAGSLAIPLLVIEIELKLSDEIK
                     TAILQRLPPSSISGADL"
     gene            complement(9145..12351)
                     /locus_tag="PLO58_06545"
     CDS             complement(9145..12351)
                     /locus_tag="PLO58_06545"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HQO74483.1"
                     /translation="MKFKITLVFIICLVLSGMMNFGLAQSDQPLIFSFKQNHTVLYKI
                     AKCSNCDNDENFDVIGIAGLVDSHGQLVPRSTYLVHLEESENSDFTVQWSYNLPQDIK
                     GDFTDIAVINWNNVPAIIAVLNITEAGTGPDPGWLLLFEYSDGFSKEPTVRMATDTVF
                     PIRPRPSYLSVGDLDHDEQSDLIISSSSPNRSISAVTQAADSSAHSLKLINYSSDLPV
                     LLGLKPFRALAANVDTEPGDEIVIFGGQQKLTVEVYRSDLKNPLSSYTFSNVNRNDIN
                     LDQLAAGDLDGDGLAEFILPLRSGGAQLLVKGVQSYKSGLLIPKKNKIAALNLVDING
                     NGLDEILWADAAGADIYRYEYNPGANLFDLDTYISFNYSNPVLTNVQLLSIAPVVSYG
                     GKNTGAIITPFLHRSFNQHGLCYWLLEETSPLIDESLIETILGSVDSVLAHKEFDQKK
                     GASSLTEMDLLKQQLGSLVGTEIELSPLPEGKGRPSGISADRQIYQPDILLHPGELMR
                     YNLNLPDLSLDDTKKLSVNIDAPSGMKFDLANKLFTWVPADSQLGLHKVTALISWDEK
                     KDVQTFTVYVNSIPKILTKIPIRDIIQIGETFSLQIEVEDANQDAAIFYKLLEFPAGA
                     SINQKGELLWKPSFDQADWYDFIVEISDGYDSEQKSFALFVNHPVSIQSTAPKTTTIG
                     KKYTYQPVISDNNKGFYLTGYSLPPRIENWSQTGVLETQILDKNIRNNISKYIENYRK
                     SFPLFITPAKMNDVPQSLFQDIFIDSEKVVFIYNLRTAKGMEAGQIIETFFSNLKMSI
                     PKYSTPVRRYLYIFNLKEPIAGLTMTGEGLITWVPAANQFDYQLISYTVTDGFFSAEE
                     QAQVYVNYPPKITSKPVTIASVNTLWQYEVKVTDLNTDSKLTYELLSAPEGMVISPQG
                     IISWQPTDLQINSYNFTIKVSDGFAQVVQKVHLFVNVKPKITSVPKPVALTNLKYEYK
                     LEAEDANNDKLTYKAVRLPKYASFDPTTGMLVWTPRKDQIGANDIVLKVTDSHGGSTL
                     HEFQVHVFANPARRHSFLRRTISLLTIAGVIYLVVIYL"
BASE COUNT         3691 a         2577 c         2564 g         3662 t
ORIGIN      
        1 aaaccctccc acaattctga cattcaaact cgtaaattgg cattttagct aaaattatta
       61 atcaaacatt taattaatct agataaatag acaagcaatt aaatcttacc aacttggctt
      121 catagattaa gaattacact atttttaaaa aatagcgatc tgaggccggt tacttcagtt
      181 tgcctgactt taacttggta tataattctt cggctttatc agggaaaatt atcgcataaa
      241 gaccgaccag caaaccgata ataccgacta catcaactaa gttcaaatgc tccagcacta
      301 acaaaattaa gctaatcgca acaagaacag cgccaaaacc tttcataaaa ataccccttc
      361 ctgccgagat tcaaaggtca taatttcaat agtgatacta tgtgttttta aattgaaaat
      421 cactttttaa accaatatgt atgttaatgc cacaaataaa atgctcagta atgcttcgtt
      481 attaaagggg acaataagac ttcctcgatt ggcaatttct ttctgaatta gtttctggat
      541 gcttttcact tttttgtccc aaactgcata gaatacttaa aaacgtttaa atataaaaag
      601 tttagcccaa actatcaaat gcaaacttaa tttgtttgat atatatcatt tatctgaatt
      661 ttatcgacga ggctataaat ctcatttgat tttcagcttt gccgaatgta tattcaataa
      721 aaaatgagag ttggttatga atagaaaaca atttttcccc tttgtctttt ttatggtgct
      781 ggctggcagt ctatcggctc aaggtgattt aggaattcat cagccggtta atctggtcac
      841 cataccgact gctggtatat tgccgcgtgg ttcctatgcg gttgattgtc atcttttccc
      901 aggcggagga ttagcgggta agataagcgt aggagttttc gaaagattta tgttcggagt
      961 atcttacggc ggtggtggga ttattggtaa ccagaagatt aattggtaca atcagccggg
     1021 cgttgagatc aaatatcgct ttatcgaaga gagcgttaaa tttccggcct tactggttgg
     1081 tttttcgtcc caaggtcatg gaaattatat tgattcactg aagcgctatg aaaccaaagc
     1141 ctctggattc tatgttgtcg tcagcaaaaa tttcaatttt ctaggcaatt taggtcttca
     1201 tggcggaatt aattataatc cgatcgagaa aacggatggc gatgccgacc ccaatttttt
     1261 cctgggtata gataaagacc tgaactccga aattgctttg attatcgaat atgatgcggc
     1321 tctaaacgac aatcacacgg aattaccgct gctcggtaaa gggtggggct atttaaatgc
     1381 cggtgttcgc tggagattgg tagataagtt cactgttgaa ctagatttca ataatatcct
     1441 gctgaaccgc aaagacgtgg actttatgaa tcgtgaattg aaattcactt ttactgaaat
     1501 attttagcct cccggagata tcgcctcatc tatacatttt aaacaacggt cataatcttt
     1561 ctcatcaaga aaataaacag aggttcctat gcgaaaatta tttttctcta attcaaccag
     1621 agtgtttttc attgttttgg gattatgtgt cttaccggtt tatctgactc ctcagtcaac
     1681 taaagtaaaa tcaatgctcg aacaagcaga taaattgcac gatgccagag attatcaggg
     1741 cacgatgacc aaattgcgcg aagccgaaaa aattgaacct aataacccag aagtactatg
     1801 gagactcgcc agagcttatt ttgatatcgc tgatcaacag cccggcgtaa gcgaagtcca
     1861 aaaagccaat ctctaccccg gctttgagta tgccaaaaaa tgcgtagagc tggcgccgga
     1921 agtagcaggc ggacatcagt actacgccat tttaatcgga caaatcggcg aactggaagg
     1981 caccaaacaa aagatcacca actcctatgc cgtgcgtgaa catactgaaa aagccattgc
     2041 attagatccg aaagaggatt cgaattacca tgttatggga cgctggcatt ttgaattagc
     2101 cgatttaagt tgggctgaac gccaggttgc cagcctgatt tatgcaacgc cgcccaaagc
     2161 ctccttcgaa gaagccgaac agtattttca gaaagcacat gaacttaagc caaaggaaat
     2221 tcgtcatcta ctctggctcg gtaagactca actaaaactc aagaagaaag atcaggctaa
     2281 aaagaatctc caattggctt tagaaattcc ggcgcaatcc gattctgata aagttatgca
     2341 gaaagaagcg gctgatttac ttaaaaaatt ataattctat gggtgctata tgaaatactt
     2401 aaaactaatt ttcaaaatcc tggctgtaat cacagtcata ggtctgttaa ttctggctgt
     2461 tctacttatt atccagaaaa aaagtaaatc ggaatccgtc gctgaagaac cggcaactga
     2521 ataattgttc ctcgacaatt ttcagatatt gttaagtaaa tatcccagac cgagcgacta
     2581 aaaattgggt gctttatttt aaatcgccga acgatttttt tcttaatctg atgatttttg
     2641 gggaaagcgg actcagaaca gccacttcaa gccaagtgtc ggtagaataa atattacatc
     2701 ctcggaaaaa acctgattag tatgccaggc acctgagaag ttaaaactaa tttcgatctc
     2761 gcctccaacg gaaagtgccg gggtgagcct gtaccaaaac tggggttcgg ataaaatgac
     2821 aatttttcga ggcggatagg catctgctcc ggtattccag atgtctatga atccactaag
     2881 ttccaatttg cgccagatat aactccaaac ataagtcaac tgaaaggtta atccgtcggt
     2941 atttagttct ttacgcagaa gaaagtcaat tggaaaattt tgcgttccaa tttgaaaaac
     3001 gtattgtaac ccgccaagcc aaaccgaatt gaaagaaccg ctatttgtca agccatcatt
     3061 atattgtaac gtagccgaaa gatcattcat tttcggcagt tggaaatagc gagctatctc
     3121 accgtaagcc agagaagcat ttcttgaacc ggacgaacct tcattattat aatcgaaatc
     3181 aacaaagaga aaggtcgcac ccagtcggtc ggttttatat agctcgtaag tcgaggttaa
     3241 atgttgacga ccaaaatcat aatggagttg cagattttgc gtcagtgcca aacaaggtag
     3301 agtaaagacc agtattatgg atgctttttt attcgttatc ttctttcata tacaacttgt
     3361 agaatttctt caattcttta ttgaaaaaag ccattttttt ctcaagattc gccagactga
     3421 taaaacctaa tccgattgcc ataaaaatca gtaataccac taatgccgtt acaattcctt
     3481 caatatataa tttatgtccg ccgaaaatgc tcggacgcaa tccaattcca ataaaaatta
     3541 ttaatccacc gataaatccg gctgtaatca ttaccacgcc aatttgaccg ctctgcccct
     3601 cagtattgcc agcaaatgtc acgataagcc atataatcat tccgagcatt aagcaaatta
     3661 agccgatata aaagtgacca agtatggttg cgataccaaa agccactagg gcgattccca
     3721 aaattatgta actatacaaa atgcgactac tggatgtcat aatttacctc ttctttttta
     3781 tttgctaagt caataatccg tccttccgtg tgattattaa taaccggttg agctttgatg
     3841 gcttcgatta gaatgtcacg cccgatgata tcggtaggag taatttttac cgcggaaggc
     3901 gctagaccga agaaacgttc gaactggcta atcacgaaat tattcgtagc gaaagtataa
     3961 attttttgat cgtcgaccgg tttgcccgca acggtcaatt ttactaactt tttttgttta
     4021 ctattaaatt cataagaaag tccagaaacc tgtaaaaggt cacgcgggtt ttcgacgtga
     4081 tactgaatga tgaacttcaa ttgttctccg gtcaatgcga ctattacgat cgtgttatcg
     4141 aatggtgaaa tttcccagag atcacgcact cgaattggtc ccgccttcaa gtctttacgg
     4201 atgccgccac tattcataaa ggcgatatca gtcttaaaat attcacgagt tacatcgcaa
     4261 atccaattgc cgagattcga ttcaccgtgg ctattgcggt gccagtcggt tatcaattgt
     4321 ccaatgattt tatccatttc tgcagagata gaagcctcaa gggagtcaac tactccggcg
     4381 acaattggtg acggttttac tctcccgaga cgggtttcta ttaattcgta attataggac
     4441 gtgatgattt tctgatttcg atctatattg gctttcaaat aacccagaca atgaccattg
     4501 gacccggctt ggcaaatcaa gatattgttg acctttttag gctggtgcaa gtaggtatgg
     4561 gaatgtccgc cgataataac atccacaccg gcaagttgca tagccaggag cgaatcttct
     4621 tcaaaaccgc aatggctcaa cagaacaatt aaatccgttt gaggttcgac ttcgttcaca
     4681 tattggcgga tttgggtcaa cgggtcaagg ataccgatgc ctttgagatt ttgcggcaaa
     4741 gccagggtga acaaatctcc agttagagca ccaataatag cgacttttaa attcccagat
     4801 tgaatgatag tataaggttg gactaataaa tcgtttttcg ttgagtcaaa tagattagaa
     4861 ttcaggatcg ggaagttggc ataggtcatc acatttcgaa gattatcaat accataatcg
     4921 aattcatgat tgccgagtgt gaaagcagtt ggttggactt tattcaagat caaaatttgc
     4981 gatagacccc gagttaaatt cgaaactggc gagccttgaa aatcatcgcc agcgttcaga
     5041 atgatcgcct ggggatgaag atggcgaagt gaatcgaagt atccagctag attagcgtaa
     5101 ccacctactc gatagtctgc ctgactggat ttataaggaa ggttagtact gtggaaatca
     5161 ttccaatgga caatgacaac ttgggggact tcggcgatca actccgccca taaattgatc
     5221 accataatta gaatcaataa agatattcca tttgcgaacc tttggcttag ccagtttttt
     5281 tgaatgaaac ataccttttc cataatcaag aatttcatta tattgacggg caaaatcaag
     5341 ttttttaaaa tttattcgat acagaacaat ttaggagaat gtctatatat tatgcttaaa
     5401 atcggtgtag taggtgctgg caactttggg cgctggcata ttaaaaattt acaaaatttg
     5461 cccaatgccg aattagtcgg tttctttgac aacaaccctg agcgggcatc ctatatatca
     5521 aacgaatttc aggttcgagc ttatccagcg ctggaacaat tgctggacga ttgcgaggcg
     5581 gtttcgatcg tggtcccgac tcattcccat tatagcgtgg caaaagccgc tattgactgc
     5641 ggacgacact tactttgcga aaaacctttt atggagacag ttgcggaagc tgaggaaatt
     5701 atccggctgg ctcgtgcgaa aaatctcgtc ctgcaggttg ggcatgttga gcgttttaat
     5761 ccggctttag gcgcattgaa acagtatcaa atagatccgt tgttcatcga gtcacaccgg
     5821 attgcacctt ttcgtggtcg tggtacagac gtcgcggttg ttctggaact tatgattcac
     5881 gatattgacc tggtgctgga tttagtcacc gcaccgatag ttcaggtgga agcctccggc
     5941 gcgccggttc tgaccgacaa tattgatatt gccaatgctc gtttgtgctt tgccaacggt
     6001 tgtgtggcta acgttactgc cagccgcgta tcgaacaaac aaatgcgcaa gttgcgaatt
     6061 ttccagcggg atgcttattt ctcgattgat atgttagccg gttccaccga aatctattct
     6121 ttatctcgcg atgccggacc ggtgcctgat gatactactc aaattatgga agtgccggat
     6181 gtgaacggtg ccccccgacg gattttctac cgcgtccaac aaaccgaaca gggtaacgcg
     6241 ctttataatg aattggagtc cttcgtcaat gcaattctaa ctggtcaacc gccgcgcgtt
     6301 agtggtgaag atggaatacg cgctctgaaa gttgccatcc agattataaa ttctattcaa
     6361 gataatttta ggaaaatcaa ttaatggata ttcgtcgtct ttttttcaaa atacgcggtt
     6421 acactcctat tccacttatg ttagttctga tttaccagtc cgatatgaac tttaaatttt
     6481 ctataatcgg agcaattttg gctttggtcg gtgaatatat ccgactgaat ggggtacggg
     6541 cggctggcgg tcgcacccgc actcgcaacg tgggcgccaa agaactttgc acctggggca
     6601 tttttgccca tacacgcaat ccgctttata tcggtaatgc tttgatttat atcgggatgg
     6661 tgtttttcgc gggtgggcgc tggcttctgc ctttgatgtt attagtgttc gtttacattt
     6721 tatttcagta tggcttaatt atatctctcg aagaagaaac gctgactaat attttcggtg
     6781 cggaatatgc aaaatataaa gccaatgtcc cgcgcttatg gccgcgtcta aaaccctgga
     6841 atagtggagt tcagccaaaa tttctgagca atcggaaatt actacgtacc gaaagatcga
     6901 cactgtcagt ttttgtaatt tttgctttag ctgtaatcct caaagagtgg ctaaatgtcc
     6961 gctggtaaac tttttatctc tgccggtgaa ctatctggcg atatgcatgc tggcaaacta
     7021 atttccgccc tgaaagaatt aaatcctgat atcgaaatca gcgccatcgg tggagataat
     7081 atggcggcgg ctggcgctga actgcttttc catattcgcg aaacgtcttt tatgggtttt
     7141 gttgaggtta ttaaacatct accatttatc atcagtatgt atcgtaagac gttgcagttt
     7201 cttgatgaat ttaaacccga tgtcgtcgtt ttaattgact atcccggttt taacttacgc
     7261 ttggctaagg cgctcgaccg gcgtgatata ccagtgattt attatatcgt acctcaagtt
     7321 tgggcttggc atcaatcccg ggttaagaag attcgccgcc acgttcagga agttatctgt
     7381 attttaccat ttgaagccac ctggtttagc gaacgaggag tgcgggcaac atttgtggga
     7441 catcctttat tagaccagaa tgaaatttct ataccggaac caaaccagaa aatttttaaa
     7501 aacggcgagc cgctggtcgg attgtttccg ggcagtcgtc agcaagaagt tgataagcat
     7561 ctaccgctaa tgatttcatc ggtcaaagat ctgcgccagg aatttccaaa tttacaggcg
     7621 gctgttgcgg ttgcttccga cgtcaatctg gaaaaatacc aaaaatgcta tcagttcgat
     7681 tggctaagct ggcatttcaa tagtacccgt tctgtgattc aatctgccga cgtcctaatt
     7741 atggcttccg gtacagttac cctcgaagcc acgcttagta acaagccttt cgtcgtaatt
     7801 taccgcgtgg caccgctgac ttactggctg gggaaaaaac taatcaaggt gccctttatt
     7861 tcgattacca atttgatcgc tgggaaaaag ggtgtaacgg aattgattca gcacgatgct
     7921 aatgtagaaa acatcaaaag tgaaatcatg agaattctgt gcgatcaatc atatgcgaac
     7981 caaatccgcg attttttgca atatgtctgc cgcctgttag gcgaaccagg tgcttcccga
     8041 cgtgcggctc acattatcct gaattattta gaatcataaa tacattaata gaaaatgcaa
     8101 ttaggtgcct taagatggct tttatttcca gcggctgtta tatacggttt gatcacaaaa
     8161 ttacggaatc tgttttacga ccgtcatatc tttaaagcta tcaaagtgcc cgttccagtg
     8221 gtcgccgtgg gcaatattac cgctggtgga actggcaaaa caccgtttgt aattgcgctg
     8281 gcaaaatttc taagtgaaaa aggttaccga attgccatca tctctcgagg gtatcgacgt
     8341 aaatccaggg gtacagttat tgtttccgat ggcagacaaa ttttaactac tccacaacag
     8401 gctggcgatg aacctttttt gatggctggt aaattgcccg gagttgtcat agttgccgat
     8461 gctgatcgta tagccgccgc ccataccgca atcgaaaatt ttgcttgcaa tttgattatt
     8521 gccgatgatg cattccagca tcggcgactc gcccgtgaca ttaatatcgt actttgggat
     8581 ccgaaaacac cgtcctggca aaagtacctt tttccggtcg gtcggcttag agaatcctgg
     8641 cacggattaa aacgcgccga ctatttagtt ttttctaaga cggataaaat catccccgaa
     8701 caacgaacct tcttcgagaa atttaatccg gtcatatcgt tttttacggc tcctctaaaa
     8761 atcgttggtc ttcgcaccct ttccgcagaa aatgtttcaa tcaatactat cgccggtaaa
     8821 aatgtgctgg cgttttgtgg gcttggcaac catattcagt tcgagacaac cactcgtcaa
     8881 ttaaactgtg gtaaggtgac attcctacga tttcccgatc accatcatta tacgaccacc
     8941 gatttgcaga agataataat caaagccgag cagatgaact gtgactatct ggtcacgact
     9001 gaaaaagacg ctgtcaacct gccggtaaat gctggatcat tggctattcc gcttttggtt
     9061 atcgaaattg aactaaaact atctgatgaa ataaaaacgg ctatcttgca aagattaccg
     9121 ccttcatcta tttccggagc ggatttatag ataaattacg actaagtaaa tcacaccggc
     9181 aatagtcaag agcgatatcg tccgtcgtag aaaagaatgt cgtcgcgccg gattagcaaa
     9241 gacatggacc tgaaattcat ggagagtact tccgccatgt gaatctgtga ctttcagcac
     9301 gatatcgtta gcccctattt ggtctttgcg gggtgtccag acaagcatac cggtggtcgg
     9361 atcaaaactg gcatatttag gcaatcggac tgccttataa gtcagcttat cattattagc
     9421 gtcctccgcc tcaagcttat attcatattt taaattagtt agcgctaccg gcttaggcac
     9481 agaggtgatt ttcggcttga cattgacgaa taggtgtact ttttgaacaa cctgggcgaa
     9541 tccgtcactg accttgatcg taaagttata agaattaatt tgtaaatcgg ttggttgcca
     9601 ggaaattatt ccctgaggag aaattaccat accttcggga gcgctgagca attcataagt
     9661 tagtttgcta tctgtattta ggtctgtaac cttgacttcg tactgccaga gggtattaac
     9721 agaggcaata gtaacaggct tggaagtaat tttcggcggg taatttacgt aaacctgagc
     9781 ctgctcctct gctgagaaaa agccgtcggt caccgtatag gaaattaatt gataatcaaa
     9841 ttgattagcc gctggaaccc aggtgattaa tccctcgcct gtcatcgtca atccggcgat
     9901 cggttcttta agattaaaaa tatagagata acgacgaacc ggagtagaat attttggaat
     9961 gctcatcttc aaattactga aaaacgtttc aatgatttgc cctgcttcca tacctttagc
    10021 ggttcgaaga ttataaatga acacaacctt ttcgctatca ataaaaatat cctggaaaag
    10081 actctggggg acatcgttca tttttgccgg tgtgataaat aatggaaaag attttctgta
    10141 attttcaata tatttgctaa tattattgcg aatatttttg tcaagaatct gagtttcgag
    10201 gacaccagtt tgactccagt tctctatccg gggtggaagc gaataaccgg ttaaatagaa
    10261 tcccttatta ttatcgctaa tgaccggctg ataggtatat tttttcccaa tagttgttgt
    10321 cttaggggcg gtagattgga tgctaaccgg gtgattaaca aataaggcaa aagatttttg
    10381 ttctgaatcg taaccgtcgc taatttcaac tataaagtca taccaatcgg cttgatcgaa
    10441 agagggtttc cacaataatt ctcccttttg attgatactg gctccggcgg gaaactcaag
    10501 caatttataa aaaatggccg catcttggtt ggcatcctcc acctcaattt gcagactgaa
    10561 tgtttcacca atctggataa tatcacggat agggatcttc gttaatattt tggggatact
    10621 gtttacatat accgtaaagg tctgcacatc ttttttctca tcccatgaaa tcaaggcggt
    10681 aaccttatga agccctaatt gggaatcggc cggaacccaa gtgaacaatt tattggctag
    10741 gtcgaatttc atacctgatg gcgcatcgat atttacactt aattttttag tatcatcgag
    10801 gcttaaatct ggaagattta aattataacg cattaactcg ccgggatgca atagaatatc
    10861 aggctgataa atttgccggt cggctgaaat acctgatggt ctacctttac cttccggtaa
    10921 gggagaaagt tcaatttctg taccaacaag gctacccaat tgttgcttta ataaatccat
    10981 ttcagtcaac gaagatgcac ccttcttctg atcaaattcc ttatgagcca gtactgagtc
    11041 aaccgatccc agaattgttt caattaagct ctcatcaatc agcggacttg tctcttccag
    11101 aagccaataa cacaatccat gttggttaaa actgcgatga agaaacgggg taattattgc
    11161 ccctgtattc ttgcctccat aagaaaccac cggcgcaatg gataataact gcacattagt
    11221 taaaaccgga tttgagtagt taaaagaaat ataggtatca agatcgaata aattcgctcc
    11281 tggattatat tcatagcgat aaatatcggc accggcggca tcagcccata aaatttcatc
    11341 gagaccattg ccgttaatat caaccaaatt aagagccgct attttgttct tttttggtat
    11401 taatagacct gatttgtagc tttgcactcc ttttactaaa agctgagctc ccccgctacg
    11461 taaaggtaga atgaactcag ccaaaccgtc accgtccaga tccccggccg caagttggtc
    11521 gagattaatg tcattacgat tcacattgga aaaagtatat gaagataatg gattcttcaa
    11581 atctgagcga taaacctcaa ctgttaattt ctgctgtccc ccgaaaatga cgatttcatc
    11641 acctggttca gtatcaacat tagccgccaa tgcccgaaaa ggtttaagac cgagcaaaac
    11701 aggcagatca ctggaataat taattaattt aagagaatga gcgctactat cggcggcttg
    11761 agtaactgca ctaatactcc gattggggct ggaggatgag ataattaagt cggactgctc
    11821 atcatgatcg agatcgccga cactgagata gctaggacga ggcctgatag ggaagacggt
    11881 atcggtggcc atccgaactg taggttcttt actgaatcca tccgaatatt cgaaaagtaa
    11941 taaccaacct ggatcaggac ccgtgccggc ttcggtaata tttaataccg caataatagc
    12001 tggtacattg ttccaattaa ttacggctat gtcggtgaaa tcacctttaa tatcctgcgg
    12061 caagttatag ctccactgga ctgtaaaatc gctattttcc gattcttcca gatgaaccag
    12121 ataagtagag cgcggaacta attggccatg gctatctacc aagcctgcaa ttccaattac
    12181 atcaaagttt tcatcattat cgcagtttga gcatttagct attttataaa gaaccgtatg
    12241 attttgttta aaagaaaaaa ttagcggttg atcagactgg gctaaaccga aattcatcat
    12301 tcctgacagc actaaacata ttataaatac gagcgtaatt ttaaatttca tctttccttc
    12361 ccatatattt taaataaaat attttatcac catttgaata tatttgccaa tagagaaaaa
    12421 atcaagctaa aagttattca gggaagcata acaaatagtg tggcgctcac ctggtataat
    12481 ttaaagcttg tgat
//
LOCUS       DAOMJU010000058        12472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5118_length_12472_cov_23.036643, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000058 DAOMJU010000000
VERSION     DAOMJU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5118_length_12472_cov_23.036643"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..819
                     /locus_tag="PLO58_06550"
     CDS             70..819
                     /locus_tag="PLO58_06550"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74484.1"
                     /translation="MLFVDSGPFGRFPSLQPVTWVDGWPIVGVDGKGVVTYRKPDVGK
                     RHPIKTLPTSDEFKDKILGMQWGWNHNPDDSRWSLTQRPGYLRLITGKVVASLPEARN
                     MLTQRIFACYDQTIPTIGITKMEIGKMKDGDIAGLAIFQNPYAFIAVKQENGIRTLIM
                     VNNGETIAETPMKGETVFLRAIASNRTKNARFEYSYDNIEFIQLGNELLMEFSLKIFT
                     GNKFGLFNYATKESGGYVDFDWFRTPMGLSN"
     gene            complement(1016..2569)
                     /locus_tag="PLO58_06555"
     CDS             complement(1016..2569)
                     /locus_tag="PLO58_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQO74485.1"
                     /translation="MEKTILISETAKETRVAILENGELVEFFVEKIDKNRLVGSIFKG
                     KVENVIEGIQAAFVDIGYESNAFLPFSEIEDSSSLAYIENISRDEEEDEQDIEDDLKP
                     TKKIHLRRGDINLQKDQEILVQVIKEPFAKKGPRVTTNISLPGRFLVLVPHAEYIGIS
                     KKILNPNEKRRLRRIAKSIKPPEFGLIIRTVAAGKDVKSLRADLDSLMEKWKQLEEKV
                     KKSKAPACVYTDMELSSTIIRDLLTPDVDKIIVDTKDLYRNILSYIKSTSPEFVNKLE
                     YYHSKNPLFEQYNIEKEFEKSLNKKVWLKSGGFIVIEHTEAMTTIDVNSGKFIGKNDQ
                     EVNSLKINLQAAKEIARQLRLRDVGGLIAIDFIDMEMEENKRRVVQELKRELQKDRAK
                     VSVANISDYGILEMTRQRTRLNLLQSVSEECPTCHGSGRVTSKGSMVTKIESWFRRFK
                     LKSNERRLDLHVNPDFAHYLKDTTNKILWKIQWRNVLRIKLVEDSNVNIDEFRVYLRK
                     SGRDVTDEY"
     gene            complement(2589..>3872)
                     /locus_tag="PLO58_06560"
     CDS             complement(2589..>3872)
                     /locus_tag="PLO58_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HQO74486.1"
                     /translation="FMLGLPTETEADLEAIISLVQKVLAVSGKRLELNITLSTFIPKP
                     MTPFQWVAMDEPLAIQRKLDYLKPRLRALKNVKVMARDPRASELEGALARGDRRLAGV
                     IETAWRAGAGFDGWQEYFDPVKWDEAFRINNLDKRVFTGELETEKALPWEHIDCGVSR
                     EYLFDEYCKARQESNTSDCRYGCNQCGVCFSGGSEMILAEEWLPPQTNPLIIKTDSEL
                     IPIRYRLHYRKFGNAVFTSHRDILRIMPGILRRAGLEPVFTQGFNPRPKLSAGYPLPF
                     GYASEDEVLDIYLKNAIENLAERLNRALPDGFEVVKSTEVTLQLPSVFAATTGFQYLV
                     QINECIDCELLRRIEEFLTAREIEFQRETSKSQQKINLRSFVETIRCKEPNLQIVTKV
                     INGRTAKIKEILQTLNIDGTNCRIVRQKTYLTPVE"
     assembly_gap    3873..3972
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3973..4878)
                     /locus_tag="PLO58_06565"
     CDS             complement(<3973..4878)
                     /locus_tag="PLO58_06565"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HQO74487.1"
                     /translation="MRIILTQVEKPGRYVGNELNAIRKDHTRAAATIALAFPDLYDVG
                     MSYYGFQILYHILNRETDIAAERVYVPWSDMSRQMRSRQIKLFSLETHTSLDQFDVIG
                     FTLPHELTYANILTMLDLAQIPPKAINRKPSDPFIIGGGSGAYNPEPLAPFFDLFVIG
                     DAEEIVVPLVRLLAKGRRQSTPRQVILEEALHRFAGIYVPTFYEVENGRTIPATSFAP
                     AVIRANKIRTLQNDYYPQAPLMPLIEIAQDRLVIELMRGCTEGCRFCQAGMIYRPVRE
                     RQPADIIRQIDQSLPQTGYDEVSLLS"
     gene            complement(5009..5569)
                     /locus_tag="PLO58_06570"
     CDS             complement(5009..5569)
                     /locus_tag="PLO58_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQO74488.1"
                     /translation="MNYPDAYELLCRYTKTESLRKHALAVSAAMRHYARIFNEDEEYW
                     AITGLLHDFDYEMYPDEQNHPYRGNEILAKEGYPEEMRTAIMGHASYTGIKRESLLAK
                     TLFAVDELSGFIIAVALVRPSKSIFEVEPRSVQKKFKDKAFAAKVNREEIKQGITELG
                     VDEAEHIQNVIDALKSAAAELGLAGS"
     gene            complement(5588..5869)
                     /locus_tag="PLO58_06575"
     CDS             complement(5588..5869)
                     /locus_tag="PLO58_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74489.1"
                     /translation="MKKSLPNPEELSLENQRMLWLRVYTDRIIRQLQTMSLDEPGAVS
                     LLEQVKQEVLQRFPDKERAYCLIYERRFKRILQRRGLFLPLTMDNGEIN"
     gene            complement(5866..6669)
                     /locus_tag="PLO58_06580"
     CDS             complement(5866..6669)
                     /locus_tag="PLO58_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQO74490.1"
                     /translation="MRIAIYQFAPIWGEINNNIENISRAIASQAADLWILPELCTTGY
                     VFSNVEELKSYAENVKEGASVKALCALSHQYQTALVFGMAEKSGDQIFNSAVVLDKGA
                     IRAVYRKVHLFYEEKNLFSAGTETPPVIEIQGVKVGVMICFDWFFPEMARTLALQGAQ
                     IIAHPANLVLPYCQAAMLTRSLENRVFTATANRIGSEMLGAKRLTFTGKSQITSPRGE
                     LLGQLKADRPGILVRDIDPTEALDKSLTPLNDIFEDRRPEVYQLGKSSR"
     gene            complement(6724..7428)
                     /locus_tag="PLO58_06585"
     CDS             complement(6724..7428)
                     /locus_tag="PLO58_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO74491.1"
                     /translation="MTPLIEYDKKITQLIAAGRYDEALWVAEEELCYAESSYGHEHLE
                     TAKVLNNLGWICDVLNKEQSAEDYYLKALEVKIAVCGNTSHELIPTLENIINFLINHN
                     QLARAYEFLVKLIDLAGLQNKLWRLRKGAYLCQLAELEEQLGFSDKAENLLWESLSFL
                     QANYGFDHPNAGRVFAKLGAFFERQGNLARAEYCYNRSLKILKKHLSSNHPDVKFSRD
                     GLENIYSKLGLKVKIR"
     gene            7596..>8194
                     /locus_tag="PLO58_06590"
     CDS             7596..>8194
                     /locus_tag="PLO58_06590"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQO74492.1"
                     /translation="MTKQHLAKDKITQLLDRSLDYKEESTAFEHWRTCPDCQAKIRTA
                     IDCAPPWLYNLPNHTNLWAIPVSARWQFWIIGSEEVVLALTFSEKAQRRQIELLRKQG
                     LELARSPAPIFLTNTAEMIYDFLETGQPYKVTALNFCLVSTAFARQVLTWTGLVPFGK
                     TTSYGRIAEWIGRPRAARSVGGALHSNPLGLVIPCHRVLG"
     assembly_gap    8195..8294
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8295..9539
                     /locus_tag="PLO58_06595"
     CDS             <8295..9539
                     /locus_tag="PLO58_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74493.1"
                     /translation="NWLFQLGDDSTWSAPSSNDSNWLPIRVPDYWEKQGFEDYDGFAW
                     YRYHFQADSALLRQDSLILYLGKIDAADEAFLNGVCIGRSGAFPPESLSTKNNVCQYV
                     FPAHLLKPDNLLAVRVYDEGEKGGIYSGPLKIVTVESPKTPLPSGKVLQNSINIIPFT
                     NGIASCCYNLKTRTFTNFQPHIYRKFDEKSETPNLISQAKVVLFRNQEEIPLTNLVTL
                     EVGYLEGTGIVRHILGGDGFKLTIFGYCPFNLNKPFWVFYALLEGEKIDDLSLNFIYA
                     SENLDLDIGKWAYQESNSKWLTVYIFYNPNPSADDADQLFLRKYKNEHPGWQALIEEI
                     DWWQKWQAQTLLPENIQEDARKVYLQSLAVLKMAQCREPFPAGGQIVASLPPSTQNYC
                     RPRDQAFAVEALLLSGHSEEAR"
     gene            9555..10460
                     /locus_tag="PLO58_06600"
     CDS             9555..10460
                     /locus_tag="PLO58_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74494.1"
                     /translation="MNSRCGRYKIYQKKDQNQGIGVDYAVSVYRYLGNGTEESVSDEN
                     GLTIHLDNFGLMIWNIYRYVEVTEDLKFLKYYWPKISRNLADVLPTLIDVSGLVRAEP
                     GPWEKSLPYKHYTYTSACTYRGLLDAARLARQMNDENRAQYYEETAINLRINIEKKLI
                     DSQLKALKGNLEDSDPNLYADASSVEALNWIFNPQDQITRNTLELFKNYLTLANAQRG
                     YCRTRQQLITNSQEWVFGDLRIISAARKATRFEEAANLEAWVTRQALNNFGLLPQYFH
                     IRSADYLGAVPRCGLGAGAYIINFW"
     gene            10616..12454
                     /locus_tag="PLO58_06605"
     CDS             10616..12454
                     /locus_tag="PLO58_06605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQO74495.1"
                     /translation="MNSPSAETRYRLCLAIANCQDTLLVTQLTTLLDDQSPTVRTGAA
                     FALGQLASQTAKSLLVQHLFSETDTEVRQQTVLALAQFGNRSTLNFLLETNSEYLGQA
                     LIYYFQRQIYNPASLNYAVSHLQSNRHSEAYQSALALSRVQNPTVMQEYVDILKTALK
                     TAGQATRVKIVTCLAPVNFSGKAEIWTQLLTDSVPEVRLEAARNIRIVAHDPLILHQV
                     FNDSFPQVVATAIENLPDSLELIPVLQDDIRILAHHPSRAVRSALVKFLSQCGGQETL
                     ANFGLWPVETNLLPAFAEGIAASGQISGLAVLDSLSRHQFRAISTPAYLGLINLAEQF
                     CRQDSIPCEQFCNYIAAGLKSDDPVKIAVAAESMRDSTTKCENLVPLLYPPLKKFKNA
                     EYADAVIEILKTIAVRQPPDAVPFIQPLLNARDFRIRDLAYQILQEIYEANVPEIIND
                     YRGAGRPSNLKMLEKYGLRPTILLETECGNIIIRLDGYYAPFTTDAFLSAIERGFYNG
                     LNFHRVVPNFVVQGGDPRGDGWGGPGYTLLTERSPIEFNCGAVGMVRTEYDTEGSQFF
                     ITLTDQPQLNYQYTRFGEVICGMDVVKRLEAGDRILAISVVTMDER"
BASE COUNT         3252 a         2766 c         2696 g         3558 t
ORIGIN      
        1 atgcccggag taacgtatgg agttcatcag ggagcactta ttcaaaccca agctggcgaa
       61 tggtggacta tgctttttgt ggatagcggt ccctttggcc gttttccatc tttacagcct
      121 gtaacctggg ttgatggttg gcctatagtt ggagttgatg gaaaaggcgt tgttacctat
      181 agaaaacctg atgttggcaa acggcatccg ataaaaacgt tgccaacatc tgacgaattc
      241 aaggataaaa tattaggaat gcaatggggt tggaatcaca atccagatga cagcaggtgg
      301 tcactaacgc agaggccggg ttatttaaga ttaataactg gcaaagtggt tgctagtctg
      361 cctgaagctc ggaatatgct tactcaacgt atatttgctt gctatgatca aactatacca
      421 acgatcggca ttactaaaat ggaaattgga aaaatgaaag atggagatat tgcggggctg
      481 gccatttttc agaatcccta tgcttttatt gcggtcaagc aagaaaatgg tattaggacg
      541 ttgattatgg taaacaacgg cgaaactatt gccgaaactc caatgaaagg tgagacagta
      601 tttttacgcg ctatcgcctc aaataggaca aaaaatgcta gatttgaata cagttatgat
      661 aatatagaat ttatccagct tggtaacgaa ttattaatgg aattcagtct taagatattt
      721 actggcaata agtttggact ttttaattat gcgaccaagg aatctggtgg ctatgttgat
      781 tttgattggt tcagaacccc aatgggattg tccaattaat cgtaaggcaa gtcatttgtt
      841 gccgggctga atcccgagca cttatatttt ctcaacatta acctcgtctt agcttggagt
      901 ttattgcctg cctgtactat tctgctctgc gggcgcaagc aagtcgccaa tatcccttgt
      961 gatggcataa cattatctaa ctggttatga gtaattgcga attgtggtta ttttttcaat
     1021 actcatcagt cacgtcacgt cccgattttc gcaaataaac ccgaaattca tcaatgttga
     1081 cattgctatc ttctactaat tttatacgaa gtacatttcg ccattgaatt ttccaaagga
     1141 ttttattggt agtatctttc aaataatgcg caaaatcggg atttacgtgt aaatccaagc
     1201 gtctttcatt agacttgagt ttgaagcgcc gaaaccagct ctcgatctta gtaaccatag
     1261 agcctttgga ggtaactcga ccagaaccat ggcaagtggg acattcttcg ctaacggatt
     1321 ggagcaaatt taatcgagtc cgctggcgcg tcatttcgag aattccataa tcggaaatat
     1381 tggcgactga taccttagct cggtctttct gaagttcgcg ctttaattcc tgaactactc
     1441 gccgtttatt ttcttccatc tccatatcaa taaagtcaat agcgattaaa ccaccgacgt
     1501 cgcgcagacg caattgtcgg gcaatttctt ttgccgcctg caaattgatt ttaagcgagt
     1561 tgacttcttg gtcattttta ccgataaatt tgccgctgtt gacgtcaata gtcgtcatcg
     1621 cttcagtgtg ttcaatgaca ataaaaccac cgctttttag ccagaccttt ttatttaagg
     1681 atttttcgaa ctccttttca atattatact gctcgaaaag aggattctta gagtggtaat
     1741 attccaattt attgacgaat tcgggagagg tgctcttgat ataagacaag atattgcgat
     1801 acaggtcttt agtatctacg atgattttat caacatccgg agtcagtaga tcgcgaatta
     1861 tcgtcgaaga taattccata tcggtataaa cgcaagctgg tgctttgctt ttttttactt
     1921 tttcttcaag ttgcttccat ttttccatca atgaatcgag gtcagcgcgt aaagacttaa
     1981 cgtccttgcc cgcggcaacc gtgcgaatta ttaagccgaa ttcaggtggt ttaatagact
     2041 tggcaatccg tcgtaggcga cgtttttcat tgggattaag gatttttttt gaaatgccga
     2101 tatattcggc atggggaacc agtaccagga aacgccctgg tagagaaata ttggtcgtaa
     2161 cgcgcggccc ttttttagca aaaggttcct taatgacttg taccaaaatt tcctgatctt
     2221 tttgtaagtt gatgtcgccc ctgcgcaggt gaattttctt cgtcggtttc aaatcatctt
     2281 cgatatcctg ctcatcctct tcttcgtctc ggctgatgtt ttcaatatat gccagcgaag
     2341 acgagtcctc gatttcggaa aatggtagga atgcgttgga ttcatagccg atatcaacga
     2401 atgcggcttg gatcccttcg attacgtttt ctactttgcc cttaaaaatt gagccaacta
     2461 ggcgattttt atctattttt tcaacgaaaa attcgaccag ctcgccgttt tctaaaattg
     2521 cgacccgggt ttctttagcc gtttcgctga ttaaaattgt tttttccatt tacttattcc
     2581 taaaattttt attcgacagg cgtaagatag gttttttgcc gaacgatccg gcagttagtt
     2641 ccatcaatgt ttaacgtctg taaaatttct ttaatctttg ccgtgcgtcc gttgattact
     2701 ttagtgacta tctgaaggtt aggttcttta caccggatgg tttcaacgaa cgaacgcagg
     2761 ttgatctttt gctgactttt gcttgtttcg cgctggaact cgatttcccg tgccgtcagg
     2821 aattcttcta ttctccgtaa tagctcacaa tcaatacatt cattgatctg gactaaatac
     2881 tgaaaaccgg tggtagccgc aaataccgat ggtagttgca aagtaacttc cgtgcttttg
     2941 actacctcga atccgtccgg taaggctctg ttcagtcgtt ctgcgagatt ttcgatagca
     3001 tttttcaaat aaatatccag tacctcatcc tccgaggcgt agccaaacgg gagcggataa
     3061 ccggctgaca gcttcgggcg cggattaaaa ccctgggtaa aaaccggctc cagtccggcg
     3121 cgtcggagaa tacccggcat tatacgcaaa atgtcccgat gggaagtgaa cacagcatta
     3181 ccaaatttac gataatgtaa gcggtatcgt atcggaatta attcagaatc ggtttttata
     3241 ataagcggat tggtctgagg cggcaaccat tcttcggcaa gaatcatttc cgaaccgccg
     3301 ctaaaacaaa cgccgcattg attgcaacca tagcggcagt ctgaagtgtt gctctcctgt
     3361 ctggctttac agtattcatc gaataaatat tcacgactta caccgcagtc aatatgttcc
     3421 catggcaatg ctttttcggt ttccaattct ccggtaaaca ctcgcttatc gaggttatta
     3481 atgcggaaag cttcgtccca ttttactgga tcaaaatatt cctgccagcc gtcaaatcca
     3541 gctcccgcgc gccaggctgt ttcaatgacg ccggcgagac ggcgatctcc acgcgccaat
     3601 gcgccttcca attccgatgc ccgcggatcg cgcgccatta ccttgacatt tttcagggcg
     3661 cgtaaacgtg gttttaaata atcaagctta cgctgaattg cgagtggttc atccatcgct
     3721 acccattgga atggcgtcat cggcttagga ataaaagtcg aaagcgtgat attcagttcc
     3781 agacgtttac cgctaactgc cagaaccttc tgaacgagag aaattatcgc ctccagatca
     3841 gcctcagttt cagttggcag accaagcata aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nncgacagaa gtgatacttc atcgtagccg gtttgaggca aagattggtc
     4021 aatttgccgg ataatgtccg ccggttggcg ttcgcgcacc ggacgataaa tcattccagc
     4081 ctggcaaaac ctacaaccct cagtgcagcc ccgcatcaat tcaattacta accgatcctg
     4141 agcaatttca atcaatggca tcaacggagc ttgtgggtaa tagtcattct gcaaagtccg
     4201 gattttattc gcccggataa ctgccggtgc aaaggatgtt gctggaatag ttcgtccatt
     4261 ctcaacttcg taaaacgtcg gaacgtagat tcctgcaaaa cggtggagag cttcttccaa
     4321 tatcacctga cggggtgtac tttggcgcct tccttttgcc agcaaacgca ccaacggcac
     4381 tacaatttcc tccgcatcac caattacaaa taggtcaaaa aagggcgcca gcggttccgg
     4441 attgtaggct ccactaccac cgccgatgat gaatggatcg gacggctttc tgttaatagc
     4501 tttcggggga atttgagcca gatcgagcat tgtcagaata ttggcataag tcaattcatg
     4561 aggtaaagtg aatccgatta catcgaattg atcaagactg gtgtgagtct cgaggctgaa
     4621 aagtttgatc tgccgagatc gcatctgccg actcatatcc gaccagggca cataaactcg
     4681 ttccgcggct atatcggttt ccctatttaa aatatgatat agaatctgga agccgtaata
     4741 cgacattccg acgtcataga gatcgggaaa tgccagcgca attgtcgccg cagcgcgggt
     4801 gtgatctttg cgaatagcat ttagttcatt gccgacatag cgaccgggtt tttcgacctg
     4861 cgttagaata attctcatca ggtcgttcga attagtaatg gttttcaatt ttcaatacaa
     4921 taataacaat tctgaatggc agataaacaa tagaacttca acaaccgata aattttagag
     4981 ctatcgagtc agctaagaat ttcatatctc aacttccagc taatcccaat tcggcggcgg
     5041 cacttttcaa agcgtcaatg acattttgaa tgtgttcagc ttcgtccacg ccgagttcag
     5101 tgatgccctg tttaatttcc tcgcgattga ctttggcggc gaaagctttg tctttgaatt
     5161 ttttctgcac cgaacgcggc tctacctcga aaatgctctt agaaggacgc accagagcaa
     5221 cagctataat gaacccgctt aattcatcta ccgcgaataa ggttttggct aacagagatt
     5281 cacgcttgat gccggtgtaa gaagcatgcc ccatgattgc cgttcgcatt tcttccgggt
     5341 aaccttcctt ggctaaaatt tcattacccc gataaggatg attttgttca tcaggataca
     5401 tctcataatc gaaatcgtgt aataagccgg taattgccca gtattcttca tcttcattaa
     5461 atattctggc gtaatggcgc atagcggctg agacggctag ggcatgtttg cgcaaactct
     5521 cagtcttggt gtagcggcag agcaattcat aggcatctgg gtagttcatt ttctctcctg
     5581 agcaaactta gtttatttct ccgttatcca tcgttagtgg taaaaacaat ccgcgccttt
     5641 gcaaaatcct tttgaaacga cgctcgtaaa ttaagcaata ggctctctct ttatcgggaa
     5701 aacgctggag gacttcctgc ttgacctgct cgagtaggct tactgccccc ggttcatcta
     5761 acgacatagt ttgaagctgg cgtattatcc ggtcagtata tactctcagc cagagcattc
     5821 gttgattttc taggctcaat tcttccggat ttggtaaaga tttcttcatc gcgacgattt
     5881 tcccagttga taaacttcgg ggcgccgatc ctcgaaaata tcattgagag gcgtcaatga
     5941 tttgtccaat gcctcagtcg gatcaatgtc gcgcactaaa atgccggggc gatcggcttt
     6001 aagttgtccc aacagttcac cacgcggact ggttatctgg ctttttccgg taaaagttag
     6061 cctttttgcg ccgagcattt cactgccgat ccggttagca gtcgcggtaa aaacgcgatt
     6121 ttccagactg cgggttagca tcgccgcctg gcaatagggc aacaccagat tggctggatg
     6181 agcaatgatt tgtgcgccct gtagagccag agtgcgcgcc atttcgggga aaaaccagtc
     6241 aaaacaaatc atcacaccaa ctttaacacc ttgaatttca ataaccggag gcgtttctgt
     6301 cccagcagaa aacaaatttt tttcttcata aaaaaggtgc actttccgat aaaccgcccg
     6361 aattgcgccc ttgtcgagca ccacggcgct gttgaagatt tgatcgccgc ttttttcagc
     6421 cattccgaaa accagcgcag tctgatattg gtggcttaat gcgcagagcg ctttaaccga
     6481 ggcgccttct ttgacatttt ccgcataaga ctttaattct tccacattac taaagacata
     6541 accggtggta catagttccg gtaaaatcca gagatctgcc gcttggctgg caatagcacg
     6601 gctaatattt tctatattat tattaatctc gccccagatt ggcgcgaact gataaatggc
     6661 aattctcata ataataaaaa tgtagtagca gggaacaaaa tgatcatttg gaaaaagtgt
     6721 gaatcagcga attttgactt taaggcctaa tttcgagtaa atattttcca aaccatcacg
     6781 gctaaacttg acatctggat gattggaaga caaatgtttc ttcagtattt ttaaggagcg
     6841 gttgtaacaa tattcagcgc gcgctaaatt gccctgtcgt tcgaaaaaag cgccaagttt
     6901 agcaaaaacg cgaccggcgt tgggatggtc gaagccatag tttgcctgca aaaacgaaag
     6961 actttcccag agtaaatttt cagccttgtc agagaagccg agttgttcct ccaattctgc
     7021 cagctggcaa agataggcac ctttgcgtaa acgccagagc ttgttttgca atccagccaa
     7081 gtctatcaat tttacgagga attcatacgc ccgagctaat tgattatgat taattaaaaa
     7141 attaatgatg ttttccagag taggaatcaa ttcgtgcgaa gtattgccgc agacggcgat
     7201 tttgacttcc agagccttaa gataatagtc ttcggcagat tgttctttat tgagaacatc
     7261 acaaatccat cccaggttat taagaacttt agccgtttca agatgttcat gaccgtaact
     7321 gctctcggca taacacaatt cttcttccgc aacccagaga gcttcatcat accgtccagc
     7381 ggctatcaat tgggtaattt ttttatcgta ctcgattagc ggtgtcataa aaaagcctct
     7441 tgggcgcggt ctttacttat taaacattct accacaaggc aaatttaata ttaccactaa
     7501 taataactac caatattttt cggtcgagtg gacaatttga cttgttgaat tgaatagtcc
     7561 tattaaatta acctatgtta attttttaga gaaagatgac taaacaacat ctcgcgaagg
     7621 acaaaattac tcaattgctt gatcgtagcc ttgattacaa ggaagaatca acggcgtttg
     7681 aacattggcg gacctgtccg gactgccagg ctaaaatacg aacagcaatt gattgcgccc
     7741 caccttggct atataatctc cccaatcata ctaatctctg ggcgattcca gtttcagccc
     7801 gctggcaatt ctggataatc ggctcggaag aagttgtgct agctttaacg ttttcggaaa
     7861 aagcgcagcg ccgacaaatt gaattactga gaaagcaagg cttggaactt gcgcgttcgc
     7921 ctgcgccgat tttcctgacc aatacggctg aaatgatcta tgattttttg gaaaccggtc
     7981 agccttataa agttacggct cttaatttct gtctcgtttc cacagctttt gctcgccagg
     8041 ttttgacttg gaccggcttg gtgccgttcg gcaaaactac cagttacggc cggatagccg
     8101 aatggatcgg tcgcccacgc gccgcccgaa gtgtcggcgg cgccttgcat tccaatccgc
     8161 taggattagt tattccctgt catcgagtcc tcggnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnaattgg ctttttcagc tgggcgacga ctcgacctgg tcagctccca
     8341 gttcgaacga ttcgaactgg ttgcctattc gcgttccgga ttactgggaa aagcaaggct
     8401 ttgaagatta tgacggcttt gcttggtatc gttatcactt tcaggctgat tcggctttat
     8461 tgcggcagga ttcgctgatt ctttatctgg gaaaaattga tgccgccgac gaagcttttc
     8521 tcaatggcgt ctgcatcgga cgtagcggag ccttcccgcc ggaatcgctt tctactaaaa
     8581 ataacgtctg ccaatatgta tttcctgccc atttattaaa gcctgataac ttgctggcgg
     8641 ttcgtgttta cgatgaaggt gaaaagggtg gaatttatag cggtccgctg aaaatcgtga
     8701 cggtcgaaag tcccaaaact cccttaccgt caggaaaggt acttcaaaat tcgattaata
     8761 ttattccttt caccaacggg atagcatctt gttgttataa tttgaaaacg cggacattca
     8821 ctaattttca gcctcatatt tatcgcaaat tcgatgaaaa aagtgagacg ccgaacctga
     8881 ttagccaggc gaaagtcgtc ctctttcgga atcaggagga aataccgcta actaatttag
     8941 tgacgctcga ggtcggctac ctggagggta ctggaatcgt tcggcatatt ctgggtggtg
     9001 atggttttaa gctgactatt tttggttatt gtcctttcaa tttaaataag cccttttggg
     9061 tgttctatgc tcttttagaa ggtgaaaaga ttgatgatct cagtcttaat tttatctacg
     9121 cttctgaaaa tctcgatcta gacattggca agtgggctta tcaggagagt aacagcaaat
     9181 ggctgaccgt ttatattttc tataatccta atccttctgc cgacgacgcc gaccaacttt
     9241 tcttgaggaa atataagaac gaacatcccg gctggcaggc tttgatcgaa gaaattgact
     9301 ggtggcaaaa gtggcaagcc cagactttat tgcccgaaaa tattcaagag gatgctcgga
     9361 aggtttattt gcaatcgctg gcggtattga aaatggctca atgccgtgag cccttcccag
     9421 caggcggtca aattgttgcc agcctgccgc cctctactca gaattactgt cggccaaggg
     9481 atcaagcctt cgcagtcgaa gcccttttgc tatccggaca tagtgaggaa gcccgctaag
     9541 ccctccagtt tatcatgaac agtcgttgtg gacgttataa aatttaccag aagaaagacc
     9601 aaaaccaggg gattggagta gattatgccg tctcagttta tcgctatcta ggcaatggca
     9661 cggaggagag cgttagcgat gaaaatggac tgactattca tcttgataat ttcggactga
     9721 tgatctggaa tatatatcgt tatgttgagg ttacggaaga cctcaaattt ctgaaatatt
     9781 actggccgaa aatctcgcgc aacctggctg atgttttgcc gactttaatt gacgttagcg
     9841 gtctggtgcg cgccgagccg ggtccctggg aaaaatctct gccttacaag cattatactt
     9901 atacctcagc ctgcacctat cgcggcttgt tggatgccgc ccgtctggcg cgtcaaatga
     9961 atgacgaaaa tcgcgcccaa tattacgaag agacagccat taacctgcga attaatattg
    10021 aaaaaaagtt gatcgattcc caattaaaag ctttaaaggg taatctcgaa gattccgatc
    10081 ccaacttgta tgctgacgct tcgagtgtcg aagcattgaa ctggattttc aatccccagg
    10141 atcaaattac acgaaacaca ctggaacttt ttaagaatta cctaactctg gcaaatgccc
    10201 agcgaggcta ttgccgaacc agacaacagt taattacaaa tagtcaagaa tgggtttttg
    10261 gcgatttgcg gattatttca gccgctcgga aggctactcg tttcgaggaa gccgctaacc
    10321 tggaagcctg ggttactcgc caggctttga ataacttcgg attgcttccg caatattttc
    10381 atattcgcag tgccgactat ctcggcgccg tacctcgctg tggtcttggt gccggagcct
    10441 atatcattaa tttctggtga aaatgatcaa ctaccgactt ggatccagaa taatatccgt
    10501 aattttttta ttaatatttt accggacttg ttcgaagcat ccttttacgc cggagattcg
    10561 ggagattctc gttcaccaaa accgacgcac tctggaaaag gtagccgatc aatacttgaa
    10621 ttcgccctca gctgaaactc gttatcggct ttgtttggct attgctaatt gtcaggatac
    10681 attgctggtt actcagctca ctactttgtt agatgatcag tcgccaacag tgcggactgg
    10741 agcggctttt gcccttggtc aacttgctag ccaaactgcc aaaagtctgc tggttcagca
    10801 cttgttcagc gaaactgaca ccgaagtgcg tcagcaaact gtcttggcgc ttgcccagtt
    10861 tggcaatcgc tcgaccttga attttctgct tgagactaat tcagaatatc tcggacaggc
    10921 tctgatttat tattttcagc gccaaattta caatccagcc agcttaaatt atgctgtttc
    10981 ccatttgcaa agcaatcggc attccgaagc ttatcaatcg gctttagcct tgagccgggt
    11041 acaaaatcca actgtgatgc aagaatacgt tgacatcctc aaaaccgccc ttaaaactgc
    11101 gggccaagcc acccgtgtaa aaattgtcac ttgtctggct cctgtgaatt tctccggcaa
    11161 ggctgaaatc tggacgcaat tattgaccga tagtgttcca gaagttcgcc ttgaagccgc
    11221 acggaacatt cgcattgtcg ctcacgatcc gctaatttta catcaagttt tcaatgattc
    11281 ctttccacag gtagtagcaa cggctattga gaacttgccc gattcactgg aattgatccc
    11341 agttcttcaa gacgacattc ggattctagc gcatcacccg tcaagggcag tacggagtgc
    11401 cttggtaaaa tttctgagtc aatgcggtgg tcaagagaca ctggcgaatt ttggcctctg
    11461 gccagttgag accaatttgc taccggcatt tgccgaaggc attgccgcct caggccaaat
    11521 atctgggctt gcggttcttg acagcctctc ccggcatcaa tttcgagcaa tatctacccc
    11581 tgcctatctc ggtttgatca atttggctga gcaattttgc cgacaggata gcatcccctg
    11641 cgaacaattc tgtaattata ttgcggctgg tttaaaaagt gacgatccgg tgaagatcgc
    11701 cgtcgccgcc gaatctatgc gtgactctac cacaaaatgc gaaaatttag tgcctttgct
    11761 ctatcctcct ctgaaaaaat tcaaaaatgc cgaatacgct gacgctgtta tcgaaatatt
    11821 aaaaacgata gccgttcgac agccgccaga tgctgtgcca ttcatccagc cgttattgaa
    11881 cgctcgcgat tttcgcatta gagatttggc atatcaaatt ttacaagaaa tttatgaagc
    11941 taatgtgcca gaaataatca atgattaccg aggtgccggg cgcccttcaa atttaaaaat
    12001 gttggaaaaa tatggactgc gtccgacgat cttgctcgaa accgaatgcg gtaatatcat
    12061 catccgctta gatggatatt atgcgccgtt tactactgat gcttttctat ccgccattga
    12121 gagaggtttt tacaatggat tgaattttca ccgggttgtg cccaatttcg ttgtacaggg
    12181 cggtgatcca cgtggcgacg gttggggtgg tccgggttat acgcttttaa ctgaacgatc
    12241 gccgattgaa tttaattgcg gtgccgtggg aatggttcgg acagaatatg acaccgaagg
    12301 ttcacagttt tttattactc tgactgatca gccgcaattg aattaccaat acacgcgatt
    12361 tggagaggtc atttgtggaa tggatgtggt caagcgcctt gaagcaggcg accgtattct
    12421 ggcaatctcc gttgtcacaa tggatgaacg ttgaaaagtc atttgtcatt ag
//
LOCUS       DAOMJU010000059        12237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5268_length_12237_cov_20.341487, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000059 DAOMJU010000000
VERSION     DAOMJU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5268_length_12237_cov_20.341487"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..648
                     /gene="rplM"
                     /locus_tag="PLO58_06610"
     CDS             211..648
                     /gene="rplM"
                     /locus_tag="PLO58_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006854802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQO74496.1"
                     /translation="MYKTSFPKVTEIKQDWYVVDAEGVVLGRLASKVAAILRGKNKPY
                     FTPHLDTGDYVIVINADKVRLTGRKAEIKTYFKHSGYLGHERFLKYKDVFQASPEEII
                     ERAVKGMLPHNALGRQLFRKLKVYRGPVHPHQAQQPKPLVIEG"
     gene            656..1048
                     /gene="rpsI"
                     /locus_tag="PLO58_06615"
     CDS             656..1048
                     /gene="rpsI"
                     /locus_tag="PLO58_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQO74497.1"
                     /translation="MPKLEYVSLGRRKSSIARLRMQTGEGKVIINSRPLDEYFGRESL
                     KRMIIQPFEVCGLTGQFDVNVKVEGGGYTGQAGAIRLAIARALEQYDPELRAKLKPAG
                     FLTRDSRVKERKKYGLRGARRAFQFSKR"
     gene            1089..1793
                     /gene="rpsB"
                     /locus_tag="PLO58_06620"
     CDS             1089..1793
                     /gene="rpsB"
                     /locus_tag="PLO58_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQO74498.1"
                     /translation="MLMKSGAHFGHLTRKWNPKMKDYVCMTQNGIHIIDLNKTLACLD
                     EATRFIREVVRKGGDVLFVGTKKQAKEIVQREADRCGMFYVVERWLGGTLTNFSTIKR
                     SIRHLQKLEKDKSIGYGENLTKKEKLALERERIKLADLHRGIKDMRKLPDVLVVVDTT
                     YDNIAVQEARRLDIPIIAILDTNADPTIVDYPIPANDDSIQTINLIITELADAVIEAK
                     NSRLVEVPPPVEEAES"
     gene            1817..2422
                     /gene="tsf"
                     /locus_tag="PLO58_06625"
     CDS             1817..2422
                     /gene="tsf"
                     /locus_tag="PLO58_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQO74499.1"
                     /translation="MGITATQVKELRDRTGLGMMECKNALVESDGNIDKAVEILRKKG
                     IAKAEKKADRVAKEGVILSYIHPGNKLGVLIEVNCETDFVAANTDFINFVKDIAMHIA
                     ATNPLSIRREEIDPAILEKEKEIYRAQVRAQNKPENIVERIVEGKVEKFYQENCLLEQ
                     PFVKNPQITVKDYVTSIIAKTGENINIARFVRFQLGESFEK"
     gene            2445..3170
                     /gene="pyrH"
                     /locus_tag="PLO58_06630"
     CDS             2445..3170
                     /gene="pyrH"
                     /locus_tag="PLO58_06630"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQO74500.1"
                     /translation="MASIPYKRVILKFSGEALAGEKQVNFDPDIIRQIAGEIKSAADL
                     GVQMGIVFGGGNIIRGSSGVGKSLDRIKADYMGMLATVINALAMQDALEKLGLRVHVF
                     SAIQMIEVAELMIIRNARKYLDQGEIVIFSAGTGQPFFSTDSGAALRAVEVQAEAIIK
                     ATKVDGVYDKDPEKFNDAQFYDRLDYQTAIEKRLKFMDLTAITICQDNDLPIIVYNMK
                     TPGNLRKVLTGEQIGTLITRSQL"
     gene            3170..3724
                     /gene="frr"
                     /locus_tag="PLO58_06635"
     CDS             3170..3724
                     /gene="frr"
                     /locus_tag="PLO58_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQO74501.1"
                     /translation="MDEVVKDAESRMKKSLDTIQQEMAVIRTGRATPALLDPIKVEYY
                     GTTVPLRQVASIGAPDARLLIVQVFDRNAVTSVEKAIRTAELGLNPIVEGNLLRVPIP
                     QLTEERRKELVKYVHKIAEEGRVAIRNIRRDANEMAKELEKDHEISEDELHNGQEEVQ
                     KLTDKYIKLIDELVKRKEKDILED"
     gene            complement(3887..4747)
                     /locus_tag="PLO58_06640"
     CDS             complement(3887..4747)
                     /locus_tag="PLO58_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQO74502.1"
                     /translation="MWYLVALLSGFFMATADAFSKKSSGQTNALLLAWVREAYALPFL
                     LPILFFIEIPELDATFWIAMAICVAMDLVTTLLYMRAIQIAPLSLTVPYLGLTPLFLL
                     IIPTIVLGERLSVIGIIGVILIAIGTYALQIDRAKSGWLEPWLAIFKNRGSLYMLIVA
                     ICYAITATLGKLAIQHSSPFFMAIVYFGLLAIVYTPFLLITTKGKLAPLINTPSNFLK
                     IGLAMALMAITHYSAINMIQVAYMISIKRLSLLFAILYGVFWFHEQNLRERLIGGIII
                     IAGATFIAFA"
     gene            complement(4921..6687)
                     /gene="dsbD"
                     /locus_tag="PLO58_06645"
     CDS             complement(4921..6687)
                     /gene="dsbD"
                     /locus_tag="PLO58_06645"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HQO74503.1"
                     /translation="MKTRRFLVLVIFLICWNFIQANESEILSVKVNLLTPNIQADGKF
                     SAEVNVQINENWHINSNKPSDEFLIPTELRIEPSDEYKVSRIIYPEPTLKRLAFAEQP
                     LAIFEGDFNIRLEGKILQTADSLVTVKGSLYYQGCNDQVCLPPTDLPFGLEIPIRPTS
                     INSVKSTLTDITAQSQTTVGNSEKDFDIGNSLAEKGYFLTFILIFLGGLGLNLTPCIY
                     PLIPVTLSYFGGQAVGKKSKTLLLALLYVLGMALVNSLLGTLAALSGSLLGSIMTNPI
                     VLIVIAAILVTLALSMFGLYEFGVPNFLMKLAGGSRSGYLGALFMGLTMGIVAAPCIG
                     PFVIGLLTYVASIGKPFIGFLMFFTLSLGLGLPFIVLAFFAAQIDRLPRSGEWMVGVR
                     QIFGFLLIGMALYFIKPLLPTELASIILGLFAIASGVYLILFSKSGNNVRTFVFIKNW
                     LAIIAIIAGTWFLKTEKADRIEMQWQTYSLPAFESALEAKRPILLDFYADWCIPCKEL
                     NKYTFSDPEVIRLSQNFALFQIDLTGNVTPEIQTLLEKYAIKGVPTIIFINRDGREIE
                     DLRLLGFEKADSFIRRMHRALE"
     gene            complement(6857..7498)
                     /locus_tag="PLO58_06650"
     CDS             complement(6857..7498)
                     /locus_tag="PLO58_06650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012670582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQO74504.1"
                     /translation="MKFIVFILIALMASALSAQPLKEADIVAIQKKVQALPDEPVDPN
                     EVVVIETDYGKIVIELFANQAPLHAMNFKKLVKAGYYTGTTFHRVIPDFVIQGGDILS
                     RDANPSNDGTGGPGYTIPAEIGQKHLRGSLAAARLPDQVNPQKASSGSQFYICVKDLP
                     SLDRGGYTVFGRVIEGMNVVDKIVAVPRDQRDRPLKDVVMKSVYITDKKSLKK"
     gene            complement(7524..8315)
                     /locus_tag="PLO58_06655"
     CDS             complement(7524..8315)
                     /locus_tag="PLO58_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQO74505.1"
                     /translation="MIFCNLGSGSKGNCTYIRHKQKALLIDQGFSLKNLHARMETIAI
                     DEHSVGAIILTHEHSDHLKGVGVFARYHKVPVYLTEQTLNVINPEFFHKVHVCPFRAG
                     DEVTIEDFKVKTFHIPHDAIDPIGIVISAEQYKIAVVTDLGTVSESVLVQIADPDLLF
                     LEANHDLTMLINGPYPLNLKQRIKSRVGHLSNEQSLMLLQRLAGNGRLKHLILGHLSE
                     INNHPSIVRELFENNIDTNLRRYQIEIADQYRPTSIIELTAPHEV"
     gene            complement(8312..8893)
                     /gene="pyrE"
                     /locus_tag="PLO58_06660"
     CDS             complement(8312..8893)
                     /gene="pyrE"
                     /locus_tag="PLO58_06660"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQO74506.1"
                     /translation="MKKDENYYLNILKESGAILSGHFLLTSGLHSATYVEKFKILQYP
                     LLCDKLCRGIAKNFENLSPNVVLGAATGGIIISHSVGRALSTRSIFAERENNELVLRR
                     GFNIANGERVVIVDDVVTTGGSLLELIDLATKYGGYILGIGVLIDRSGGKINFGFPFH
                     SLITLEIPTYKPEECPQCLANIPLTTRGRTGKL"
     gene            complement(8901..>9571)
                     /gene="pyrF"
                     /locus_tag="PLO58_06665"
     CDS             complement(8901..>9571)
                     /gene="pyrF"
                     /locus_tag="PLO58_06665"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQO74507.1"
                     /translation="YHFVKEIIAATGAYTPAYKFNLAFFEMWGSRGWQMLEKLLSEVP
                     QNILLIGDAKRGDIGNSAKFYAKALFEALPFHAVTLSPYLGTESILPFIADDSHGAFV
                     LCVTSNPSSREIQDFGGDQHLYLKVAEIVQQLNQRRNLGLVMGATKPEQLLSVRQKFP
                     NLPFLIPGVGAQGGEIAAAVQVCRTCGVGLINVSRAVLFPDKGKFPENVAETAAYYQE
                     KFKI"
     assembly_gap    9572..9671
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9672..>12237)
                     /locus_tag="PLO58_06670"
     CDS             complement(<9672..>12237)
                     /locus_tag="PLO58_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74508.1"
                     /translation="RANAFRSRDLRSFQKYETQFEAPASINIESITINAKPVSVMQPE
                     SVNPAVILAESLQPGDSVELFIQFTTIIPPGKSKLCSTYSEKSYCLIYFYPRIAEYRR
                     GRWQITGLNELERAPLEFANYSVRFTLPDYFQPAGSLQIDSVVSHNDKNTFIFRSEYL
                     QDIGFILNDRLKVSTIRSRPHNEVKFISRFSANDKSKDKIATEIINDIVDYYAKYLPG
                     MNQQITIYPTVIPGGFTTSRLILIDKTILTEATKLDYLSLYILAREIAHQYLGFNVES
                     ENDPSVSISDGLAGFLANDYLKNRYEFLQQKYKLSDNQLISFNTKVFNVFTSGLEKEN
                     ILQPARASNERANTIFINTQTRFLKSQKLVEMSHYVLGDSLFSQVLAQYQAFIQSDSI
                     DPQVFFKIAEEKSGYEFIALYQNFLHLEQPTDLKIQKVIRSQANNSEHITDVIIKQSS
                     LNYLPLEVNAFDRSGNVYNHRIQPRTGTFDTLTLVTDKPITKIVLDPNRQIWDSNRLN
                     NQYPRSVVFKFLVGLPSIDSYQIFYYPTFDFNNRDIMRFGVKLRGRYWINMRPLFPAQ
                     SLDEWSFGLNYGYKSKTFGYDLSYSTSLLAFLFKPRIYLRWRDYFDLMEGKISTEVYL
                     GDVKYWGFNRVQGYQKLNLGLDYQKVRSLEFLNTNKWETGQSLKPYLDYVNFHNWGNI
                     RHIVHAKFVYGLPVGENHFSFDKLTLDGQVKMRLNHRIWLYQRLFYGNAHGNVPKQEY
                     FYFFGKNVLENQSFESYRLAQGEGDMRGYGDQSLKSYKMLTSNTELRRNLALAGAAMF
                     DCLIFLDSGILPESFSAIDWKQTHFDAGLGVELELLDILKVGMHFPLWVSHPPQSEP"
BASE COUNT         3750 a         2476 c         2651 g         3260 t
ORIGIN      
        1 tttaatcttt ctgtccatat ccagattagg attggcttgt cctgattgag aaaagatcta
       61 ttctggtggc tctgaaaatc gaaagaataa ttaagaatgg aatacaatgt ctatagatca
      121 gacgtgaagt cgtggaaaaa ttccttgatt ataatatcct caaatcgtaa gtttacccgc
      181 tatttttgaa ttataattaa gagataagca atgtacaaaa cgtcttttcc gaaagtgaca
      241 gaaattaaac aagattggta cgtggttgat gccgaaggtg tagttctcgg tcgtttagcc
      301 agtaaagtgg cggcgatttt acgtggtaaa aacaagccgt attttacgcc gcatctcgat
      361 accggcgact atgtcatagt tattaatgcc gataaagtac gattaactgg tcggaaagca
      421 gaaattaaga cttatttcaa gcattctggt tatctcggac atgaacgctt tctcaagtat
      481 aaagatgtgt ttcaagccag tccagaggag atcatcgagc gagcggttaa aggaatgtta
      541 ccgcacaatg cgcttggtcg ccagcttttc cggaagctga aagtctatcg cggtccagtc
      601 catcctcatc aagctcagca acctaaacct ttagtaatag aaggataagt aacggatgcc
      661 caaattagaa tatgtgtcat taggtcgtcg taaaagttca attgcccgtc ttcgaatgca
      721 gaccggcgaa ggcaaagtta ttatcaattc ccgtccactg gatgaatatt tcggtaggga
      781 atctcttaag cgtatgatca ttcagccatt cgaagtctgt ggcctaactg gtcagtttga
      841 cgttaatgtc aaagtagagg gaggtggata taccggccag gctggtgcga tcagattagc
      901 aattgctcgg gctttggaac aatatgaccc ggagctgaga gccaagttaa aaccggctgg
      961 ctttttaacc cgtgactcgc gggtgaaaga aagaaaaaag tatggattgc gcggcgcccg
     1021 tcgagctttc caattctcca aacgttaaag gcagagagat cagatttatg gtaaatgtaa
     1081 ctgtagaaat gttaatgaaa tcaggtgctc attttggtca tttaactaga aaatggaatc
     1141 ccaaaatgaa agattacgtc tgtatgacgc aaaacggcat ccatatcatt gatttgaaca
     1201 aaacgctcgc ctgtcttgat gaagcgacgc gctttattcg ggaagtagta cgcaaaggtg
     1261 gcgatgtatt attcgtcgga acaaagaagc aagccaaaga aattgtccag agggaagccg
     1321 atcgctgtgg tatgttctat gtcgttgagc gctggttagg tggaaccttg accaattttt
     1381 caactatcaa acgcagtatc cggcatttgc aaaaattaga aaaagataaa tctattggct
     1441 atggtgagaa tctgaccaag aaagaaaaat tagcgctcga acgtgaacga ataaaattag
     1501 ccgatctgca tcgcggtatc aaagatatgc gtaaattgcc cgacgtgctc gtggtagtgg
     1561 atacgacgta tgataatatc gccgtccagg aagcccgccg tttggacatt ccgattatag
     1621 ccatcctaga caccaatgct gatcccacca ttgtggatta cccgattcca gccaatgatg
     1681 attcaatcca aactattaat ttaataatta cggaactggc tgatgctgtt attgaagcca
     1741 agaattcacg attggttgaa gtaccgccac cggttgaaga agcagaaagc taaatagcta
     1801 tacagaaagg aaaaatatgg gaatcactgc cacccaggtt aaggagttac gagaccgtac
     1861 aggcttggga atgatggaat gcaaaaatgc attggttgaa tcagacggca atattgataa
     1921 agctgtcgaa attctacgaa aaaaagggat tgccaaagcg gaaaagaaag cggatcgcgt
     1981 tgccaaagaa ggagtaattc tttcctatat tcatcccggc aataaactgg gagtcttaat
     2041 tgaagtcaat tgcgaaactg atttcgttgc cgccaacaca gactttatca actttgtgaa
     2101 agatatagct atgcacatcg cggctactaa cccgctatca attcgccggg aagaaataga
     2161 tccagcaatc ctggaaaaag aaaaagaaat ttatcgcgct caggttcggg ctcagaacaa
     2221 accggaaaac atcgttgagc gcatagttga aggtaaagtc gagaaattct atcaggaaaa
     2281 ttgtctgttg gaacagccgt tcgttaaaaa tccccagatt acggttaaag attatgttac
     2341 cagcataatt gctaaaaccg gtgagaatat caatattgcg cgcttcgtcc gctttcagct
     2401 gggcgagagt ttcgaaaaat aaataacctc taacaggtta acaaatggca tccattcctt
     2461 acaaacgagt aatcctgaaa ttttcaggag aagcgcttgc gggtgaaaag caagtaaatt
     2521 ttgatcccga tataattcgc caaattgccg gggaaatcaa atccgccgct gaccttggag
     2581 ttcaaatggg aatagttttc ggcggaggta atatcattcg aggctcgagt ggcgttggca
     2641 agtcgcttga tcgcatcaaa gccgattata tgggaatgtt agcaacggtt attaacgctt
     2701 tagcaatgca ggatgctctc gaaaaattag gattgcgtgt acatgtattt tcagccattc
     2761 agatgatcga agtcgcggaa ttaatgataa tcagaaatgc ccgtaaatac cttgaccagg
     2821 gtgagatcgt catcttcagt gctggaacgg gacagccttt tttcagtact gattccggag
     2881 cggctttgcg ggcggtggaa gtgcaagccg aagccattat caaagccact aaagttgatg
     2941 gcgtttatga taaagatccg gaaaaattta acgatgccca attctatgat cggctggatt
     3001 atcagaccgc tattgaaaaa cgtctgaagt ttatggactt gaccgctatt acgatttgtc
     3061 aagacaacga cttgccaatc attgtctata atatgaaaac cccgggtaac ttgcgtaaag
     3121 ttttaaccgg tgaacaaatt ggaacgctaa ttacacggag tcaattatga tagacgaagt
     3181 cgtcaaagat gccgaatccc gtatgaagaa aagtttagat acgatccagc aagagatggc
     3241 tgtaattcgc accggacggg caaccccggc tttactcgat ccaatcaaag tcgaatatta
     3301 tgggacgact gttccgttgc gccaggtagc atccatcggt gctccggatg cccgcctttt
     3361 gattgtccaa gtttttgatc gaaacgctgt gaccagtgtt gaaaaagcta ttcgaaccgc
     3421 tgaacttggt ttaaatccca tagttgaggg caatttacta agggtaccta ttccgcaatt
     3481 aaccgaagaa cgtcgtaaag aattggtaaa atacgtacat aaaattgctg aggaaggccg
     3541 ggttgctatc cgtaatatac gccgcgatgc caatgagatg gcgaaggaac tagaaaaaga
     3601 tcacgaaatt tccgaagatg agttacataa cggacaggaa gaagtccaga aactgaccga
     3661 caaatatatc aagctgattg atgaattagt gaaacgcaag gaaaaggaca tcctggaaga
     3721 ttagtccgac gccttaacca acttcaaaat aaaatattat aagccaagaa gtcatcaatg
     3781 tttgataggc aaacgatttg tcccctgatt tgattttatc attttcaaat ttcatataaa
     3841 tcagaacggt accactatct ctgtcagcct ttaccaataa attaatttag gcaaaagcga
     3901 tgaatgtcgc tccggcaatt atgattattc ctccaatcag gcgttcccgc aaattctgtt
     3961 catggaacca gaatacgcca tatagaatgg caaatagcag gctgagtcgt ttgatcgaga
     4021 tcatataagc aacctgaatc atatttattg ccgaatagtg agtaatcgcc ataagtgcca
     4081 ttgccaagcc gatttttaga aaattgctcg gagtgtttat taatggtgct aatttcccct
     4141 tcgtcgtaat gagtaggaac ggcgtgtaaa cgattgctag caaaccgaag taaacgatag
     4201 ccataaaaaa gggtgaggaa tgctggatgg caagtttacc gagagtggcg gtgatggcat
     4261 aacaaatagc cacgatcagc atataaagtg agccgcggtt tttgaaaatt gccagccagg
     4321 gttccagcca gccggacttg gcgcggtcaa tttgcagtgc ataggtgcca attgcaatta
     4381 agataacgcc aataatcccg atgaccgaaa gccgttcgcc caatactatc gtcggaatga
     4441 taagcagaaa aagtggagtc aagccaagat aaggcacggt cagtgataat ggagcgatct
     4501 ggatagcgcg catataaagt aaagtggtga ccagatccat tgcgacacaa atcgccatag
     4561 caatccaaaa ggttgcatct aactcgggga tttcaataaa aaacaaaatt ggcaaaagga
     4621 atggcaaagc ataagcttca cgcacccaag ccaaaagaag agcattagtt tgaccggacg
     4681 attttttgga aaaggcatcc gcggttgcca taaaaaatcc cgaaagtaaa gcgacgagat
     4741 accacatgtt atttcctgat taaaattctg atttcaagtg cagaatttaa tggacagatg
     4801 caaactatac tatgaaatat agttaatgac gaatagatat aaataagcct aaagcattac
     4861 ttctgttgtg acagattatc ttattttaca ataagttcga gaaattaata gtgcaaaatt
     4921 ttattcgaga gcccggtgca ttcggcgaat aaagctatcc gccttttcaa agcccagaag
     4981 ccgtaaatct tctatctcgc gaccgtcgcg gttgatgaaa atgatggttg ggacgccttt
     5041 gatggcatat ttctccagca aagtctgaat ttccggggtg acgttgccgg ttaggtcaat
     5101 ttggaatagg gcaaagtttt gactcaggcg aatgacttcc ggatcgctga aagtatattt
     5161 atttagctct ttacaaggaa tgcaccagtc agcgtagaaa tcgagtaaga tcggtcgctt
     5221 cgcctccaga gccgattcga aagctggtag ggaataagtc tgccattgca tctcaatccg
     5281 atccgctttt tccgttttaa gaaaccaggt gccggctatg atggcaataa ttgccagcca
     5341 atttttaatg aaaacgaacg ttctgacatt gtttcctgac ttgctgaaaa gaattaaata
     5401 aacgccgctg gcaatggcaa atagccctaa tattattgaa gccaattcag ttggtaacag
     5461 cggtttgatg aaatagagcg ccataccgat taataaaaac ccgaaaatct ggcgcacccc
     5521 aaccatccat tctcctgagc gtggtaggcg atcgatctga gccgcaaaaa acgccagaac
     5581 aatgaaaggc aaacccaacc cgagtgacaa ggtaaaaaac attaagaacc cgataaacgg
     5641 tttaccgatc gaagcgacat aagttaaaag tccaattaca aatgggccaa tgcagggcgc
     5701 ggctacgatt cccatcgtta gtcccataaa aagcgcaccg aggtagccac tccgactgcc
     5761 gcctgccagt ttcatcaaaa agttaggcac cccgaattca tataaaccga acatactgag
     5821 tgccagagtg actagaatag ccgcgattac gattaaaacg atgggattag tcataataga
     5881 tcccagcaga ctgccggaaa gcgccgctaa agttccgagg agagaattaa ccagcgccat
     5941 ccctagaacg tataacaaag ccaacaacag ggttttgctt tttttaccga ctgcttgacc
     6001 accgaagtaa ctcaatgtga ccggaattaa tggataaata cagggtgtca aattcaatcc
     6061 caagccgccc aggaatatca ggatgaacgt caaaaaataa cctttttctg ccaacgaatt
     6121 gccaatatcg aaatccttct cagaattgcc aactgtagtt tgcgactgtg cggtgatatc
     6181 ggttagcgtg gatttgacgg aattgatact ggtcggtcta atcggaattt ccagcccaaa
     6241 aggtaaatca gtcggcggca ggcaaacctg atcattacag ccttgataat ataacgagcc
     6301 tttcacggtt accaatgaat cggcggtctg gagtatcttg ccttcgagcc ggatattaaa
     6361 atcgccttcg aaaattgcca gcggttgttc ggcaaacgct aaacgcttaa gagtgggttc
     6421 aggataaata attcggctga ctttatattc gtccgaaggc tcaatgcgca attcggtcgg
     6481 gataagaaat tcatcactcg gtttattaga attgatgtgc cagttctcgt taatttgaac
     6541 gttcacttct gccgaaaatt ttccatcagc ctggatattg ggtgtcagta aattgacttt
     6601 aactgataaa atttccgatt catttgcctg gataaaattc cagcatatta agaaaatgac
     6661 taataccaag aatctgcgag ttttcatggt taatgttttt ctactattga taaaaaaaag
     6721 atttcagagt tatacaccaa ttaaaaatgg aatgaaaccc tcgaaattaa aatccaggta
     6781 attatttaaa ttattcggaa ttttttgatc tttaaaaata ttcaaatgct ggttataagc
     6841 cgacaagaaa atcaattcat tttttaagag attttttgtc ggttatatag actgatttca
     6901 ttaccacgtc ttttaacggt cggtcgcgct gatcgcgcgg gactgccacg attttatcaa
     6961 cgacgttcat tccttcgatc acccgtccga acacagtgta accgccccga tccagagagg
     7021 gcaaatcctt gacgcagatg tagaattgac tgccactgga agctttttga ggattgactt
     7081 ggtcaggcaa acgcgccgcg gctaaactcc cgcgtagatg cttttggccg atttcggctg
     7141 gaattgtata gcccggtccg ccggtgccgt cattacttgg attggcatcg cgactgagaa
     7201 tatcgccacc ttgaatgacg aaatccggta tgacacggtg aaaagtcgtt ccggtataat
     7261 agccggcttt tactaatttc ttaaagttca tagcgtggag aggtgcttga ttagcaaaca
     7321 actcgatcac gattttgcca taatcggttt caattacgac gacttcgttg ggatcaaccg
     7381 gttcgtcagg taaagcctgc actttcttct gaatagcaac gatgtcggct tctttcagtg
     7441 gctgagctga aagtgcggat gccattaatg caattaaaat aaaaacgata aatttcataa
     7501 gtctccctaa tttaaaaatg atttcagact tcgtgaggcg cggtcaattc gataatcgag
     7561 gttgggcgat attgatcggc aatctcgatt tgataacggc gcaggttagt atctatatta
     7621 ttttcaaata actctcttac aatactcgga tgattattga tttcactcaa atgcccgaga
     7681 atcagatgct ttaaacgtcc attaccggct aaacgttgca gtaacattaa tgattgttca
     7741 ttagataggt gaccaactcg acttttgatg cgttgtttaa gattaagggg ataaggtccg
     7801 tttatcaaca tcgtcagatc gtgattggct tccagaaaga gtaaatccgg atcggcaatt
     7861 tgaaccaata ccgattccga aactgtaccg agatcggtca ccaccgcaat tttatactgc
     7921 tcggctgaaa tgacaatgcc aattggatca attgcatcgt gtggaatatg aaaagttttg
     7981 actttaaaat cttcaatggt tacttcatca ccagctcgaa agggacaaac gtggactttg
     8041 tggaaaaatt ccgggttgat gacgttaagc gtctgttcgg tgagataaac gggaacttta
     8101 tgatatcggg caaagacacc cacgcctttg agatggtcgg aatgttcatg agtcaaaata
     8161 atggcaccaa ccgaatgctc gtcaattgct atcgtttcca tacgcgcatg caagtttttc
     8221 aggctaaagc cctgatcaat tagaagcgcc ttttgtttgt gacgaatata agtgcagtta
     8281 cctttactgc cactgccgag attgcaaaag atcatagttt gcctgttcga ccacgagttg
     8341 tcaggggaat attagccaga cattggggac attcttccgg cttataagtc ggaatctcca
     8401 atgtaatcaa ggaatgaaac ggaaaaccga aattaatttt tccaccgcta cggtcaatca
     8461 acacaccaat tcctaggatg taacctccat actttgtggc taaatcaatt aattcaagaa
     8521 gtgaaccgcc ggtggtaacg acgtcatcaa cgattacgac gcgctcgcca ttggcaatat
     8581 taaatccacg tcgcagaacc aattcgttat tttctctttc ggcgaaaata ctgcgcgtgc
     8641 tcaaagcccg gccaacgctg tggctgataa tgatgccgcc ggttgcggct cccaagacga
     8701 catttgggct caagttttca aaattcttgg cgatgcccct acaaagctta tcgcaaagta
     8761 aaggatattg taaaatctta aacttctcaa cgtaggtggc actatgcaag ccagaggtca
     8821 gtaaaaaatg accactcaga atggcgccgc tttccttaag tatatttaaa tagtaattct
     8881 cgtctttttt catctaaaac ctaaatttta aatttttctt gataataagc ggctgtttcg
     8941 gcgacattct ccggaaattt acctttatct ggaaataaaa ctgcccgaga aacattaatc
     9001 aagccaacgc cgcaagttcg gcagacctga acagccgccg cgatttcgcc accttgagcg
     9061 ccaactcccg gtatgagaaa cggcaaattg ggaaattttt ggcgaacgct cagcagttgt
     9121 tccggtttag tggcgcccat aactaatccg agattccgac gctggttcaa ttgctgaaca
     9181 atttcagcca ctttaagata taaatgctgg tcaccgccaa aatcctgaat ttcccgactg
     9241 gaaggattgg aggtaacaca tagaacgaaa gcgccgtgtg aatcatcggc aataaacggc
     9301 aagatgctct cggtaccaag ataagggctg agagtaactg cgtggaaagg taatgcctcg
     9361 aatagggctt tagcgtagaa tttggcgctg ttaccgatgt caccgcgttt agcatcgccg
     9421 attagcagaa tattttgcgg cacctcgctg agcaatttct caagcatttg ccagccacgc
     9481 gaaccccaca tttcgaaaaa cgccaagttg aatttataag ccggtgtata agcgccagtg
     9541 gcggcgatta tttccttaac aaaatgatag annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nggctctgat tgcggaggat gcgataccca taacgggaaa tgcataccaa
     9721 ctttgagtat gtccaataac tccagttcga cacctaatcc ggcatcgaag tgagtctgtt
     9781 tccaatcaat ggcactaaat gattccggta atataccgct atcgagaaaa atgaggcaat
     9841 cgaacatcgc cgctccagcg agggcaagat tacgacgcaa ttcggtattg ctggtgagca
     9901 ttttatagct cttcagagat tgatcgccat aaccgcgcat atcgccctca ccctgagcta
     9961 accgataaga ctcaaaactc tggttttcca gcacattttt accgaagaaa taaaaatatt
    10021 cctgtttggg tacatttcca tgggcattac cgtagaaaag ccgttgataa agccaaatgc
    10081 ggtgatttaa gcgcatcttg acttgaccat cgagagttaa tttgtcaaaa ctgaaatggt
    10141 tttcgccaac cggtaatcca tatacgaatt tggcatgaac gatgtgtcgg atgttacccc
    10201 aattatggaa attgacataa tccagatagg gttttaaact ttgtccggtt tcccacttat
    10261 tggtatttaa gaactccagg ctacgtacct tctggtaatc taatcccaag ttcagtttct
    10321 gatagccttg aactcgattg aatccccagt atttaacatc tcccagatat acttcagtag
    10381 aaattttacc ctccatcaag tcgaaataat cgcgccaacg cagataaata cgtggcttga
    10441 ataaaaatgc cagcaaagat gtgctgtaac ttaaatcgta tccgaaagtc ttgcttttat
    10501 aaccataatt caaaccgaaa gaccattcgt ctaaactttg agccggaaat aaaggccgca
    10561 tattgatcca gtaacgacca cgtagtttaa ccccaaaacg cataatgtct ctattattga
    10621 aatcgaaagt cggataataa aagatttgat acgaatcaat actcggcaac ccgacaagaa
    10681 atttaaaaac taccgaacga ggatactgat tgttaaggcg attggagtcc cagatttgcc
    10741 gattgggatc gagcacaatt ttggtaatcg gtttatctgt aaccaaagtt agagtatcga
    10801 atgttccagt tctcggctgt attctatgat tgtaaacatt gccgctgcgg tcaaaggcat
    10861 ttacttcaag cggaagataa tttaacgacg attgtttgat aatcacatcg gtgatatgtt
    10921 cagaattatt tgcctgtgac cggatgactt tttgaatttt taaatccgtc ggttgttcta
    10981 aatgcagaaa attttggtag agagcaatga attcataacc ggatttttcc tcagctattt
    11041 taaaaaatac ttgtggatca atactatcag attgaataaa agcctgatat tgagccagaa
    11101 cctggctgaa aaggctgtca ccgagcacat aatggctcat ttcaactaat ttttgacttt
    11161 ttaaaaaacg ggtttgagtg ttgataaata tagtatttgc cctttcgttg gaagcccgcg
    11221 ccggttggag aatattttct ttttccaacc cagatgtaaa aacattaaag actttggtat
    11281 taaatgaaat taattggtta tcagacagtt tatatttctg ctgcagaaat tcgtagcgat
    11341 tttttaggta atcgttcgcc agaaatccag ctagaccatc actaatcgag acactaggat
    11401 cattttcgga ttccacatta aaacccaggt attggtgagc aatttctcga gctaatatat
    11461 acaaacttaa ataatcaagt ttagttgcct ctgtcaatat tgttttatca attaatatca
    11521 gacgagaagt tgtgaaaccg ccagggatga ccgtcggata aatcgttatt tgctgattca
    11581 ttccgggcaa atatttggcg taatagtcta ctatgtcatt tattatctca gtagcgatct
    11641 tatccttgga tttatcgttg gctgaaaacc ggctaataaa ttttacttcg ttatgtggtc
    11701 gagaacgaat agtcgaaacc ttcagtctat cattgagaat gaatccaatg tcctgaagat
    11761 actcggagcg aaatataaaa gtatttttat cgttatgact aacaacagaa tcaatttgaa
    11821 gtgaaccagc cggctggaaa taatccggca gggtgaatct aacagaataa ttggcaaatt
    11881 cgaggggtgc gcgctccagc tcattaagtc cagtaatctg ccatctacct cgacgatatt
    11941 ctgcaattcg ggggtaaaag taaatcaggc aatacgattt ttccgaataa gttgaacaga
    12001 gtttgctttt cccgggtggt attatagtcg tgaactgaat gaataattcg accgagtcgc
    12061 cgggttgaag agattcagct aaaattacag ccggattgac cgattccggt tgcattaccg
    12121 aaaccggttt ggcatttatc gtaattgatt caatgttgat agacgccggt gcttcaaatt
    12181 gagtctcata tttctgaaaa ctgcgtaaat cacgactgcg aaaggcattg gcgcgaa
//
LOCUS       DAOMJU010000060        12173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5296_length_12173_cov_22.718600, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000060 DAOMJU010000000
VERSION     DAOMJU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12173
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5296_length_12173_cov_22.718600"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1222
                     /locus_tag="PLO58_06675"
     CDS             167..1222
                     /locus_tag="PLO58_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQO74509.1"
                     /translation="MNKPPNRSPNVTLDDIARQLKVSKVTVSKALRDHPDIGSATKRE
                     VQALAAKLGYVPNFMARNLSARSSRTIGLVVPKLAHYFFATCIESIYNAANASQYDII
                     MTVSQENPEYELKHIQTLLAMRVEGLLVSVTENTTDTSIFQDIKKRGVPIVFFDRIPF
                     GLESNYVVTDDRAAAARLTNYVIQAGYRKIAHFAGYSHTNIGYERQTGFRQALSDNNL
                     PIREDWIIEGGFGERAGYEALKKVAETKDLPELIFAVTFPVALGIFEAASELGLKIPG
                     DIDLVCFGGGQFNRYLSPALTFMDQPASAIGEKAFNLLLDELHSSERRSEQHIVVPSQ
                     LVIGETCRKLQYNYEEL"
     gene            1219..2622
                     /gene="uxaC"
                     /locus_tag="PLO58_06680"
     CDS             1219..2622
                     /gene="uxaC"
                     /locus_tag="PLO58_06680"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HQO74510.1"
                     /translation="MILPSDRFFDSDPAIRSLARELYQQVKDLPIISPHGHIEAKILA
                     ENQPFPDPTALFILPDHYIYRLLYSQGIPLESLGIPTRDGTPVETDHRKIWQIFADNY
                     YLFAGTPTSLWLAYEFSEVFGVTEKLNSANAMAIYDHLQNQLQKPEFRPRALYERFNI
                     EVLTSTDGATDSLKYHRQIKESGWKGRIVPCFRPDALFNINAVGWRQEIEKLGDAVGY
                     EVNTYSKFITAIENRREFFKTMGAVSTDQGVESAYTHRLSASQAEEIFQRARNGKASA
                     DDARLFTANMLMEMARMSVADGLVMQMHVGALRNHNLRNFRRFGPDTGADIPVRSEFT
                     VNLRELLNEYGTDSRLTLVVFTLDESTYSRELAPLAGYYPAMKLGAAWWFHDSIQGMI
                     RFREMITETAGFYNTVGFTDDTRSFPSIPARHDLCRRVDSNFLAGLVVRHIIDLDEAH
                     VLIRALTYDLAKKTYKL"
     gene            2640..3635
                     /locus_tag="PLO58_06685"
     CDS             2640..3635
                     /locus_tag="PLO58_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HQO74511.1"
                     /translation="MRTKFLTKIGLGTAIIISGFCLNGCESEARTKTIKLAHALDTSH
                     PVHKSLVYMSERFAEKSGGKYRIDIYPSGQLGSEREYVELLQIGSLGMTKVSCSVLEG
                     FVDKMKVFSMPYLFRDDEHRFKVLEGEIGRELLLEGEKYWIRGLCYLDAGCRSFYSVK
                     KPINTPEDLRGLKIRTQESTTSIQMIQAMGGSATPIAWGELYTALQQGIVDAAENNPP
                     SFYLSHHHEVAKYFALDEHTAVPDILLISTKVWNDLPPQVQEWLQSAADEAAIYQREL
                     WKIATQEALEEVQKAGVVVSYPDKRPFQEKVKDIYESYRSDPEIYELIQRIRAVQ"
     gene            3635..4168
                     /locus_tag="PLO58_06690"
     CDS             3635..4168
                     /locus_tag="PLO58_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HQO74512.1"
                     /translation="MLKIRKILDNILKWVVVVLMAISVFNVLWQVFTRFILRNPSSYT
                     EELARYLLIWVGLLGAAYAAGNKMHLAIDLFSAKMKRNNALRLQIFIQICVLFFAISV
                     MVIGGLRLVTITLTLNQLSAALKVKLGYVYMVLPISGILIAIYSIVEIIGLFGQLFRP
                     DSTNNLPEKRTEYHGVD"
     gene            4155..5456
                     /locus_tag="PLO58_06695"
     CDS             4155..5456
                     /locus_tag="PLO58_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HQO74513.1"
                     /translation="MEWTELIVLVISFVFLLLAGVPISFSIGIATLSTMLLSIDTLPA
                     LTTVAQRMATGIDSFALLAIPFFILSGLLMSRGGIARRLIDFAKVLVGRWPGGLAYVN
                     ILAAMFFGAISGSAAAAAAAIGGFMTPLMEKEGYDTNYSASVNITSATTGLLIPPSNI
                     LIVYSLASGGVSIAALFIAGYLPGILVGLLLMVAAGIIAVKKGYRGSDERYTFKQGIM
                     RFLDALPSLMLIVIVIGGIIAGFFTATEGSAIAVLYTFILSVLIYHEVKWKELPQVLL
                     ETVTTTAIVMLLIGTSMAMSWIMSYANIPQTITASLIALSSSKIIILLIINLILLFVG
                     TFMDMTPAVLIFTPIFLPIVTALGIHPIHFGIIMVLNLCIGLCTPPVGSVLFIGCGIA
                     NTTIPKIVKSILPFYAAMILALLLVTYFPQISLFLPRLFGY"
     assembly_gap    5611..5710
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5711..6043
                     /locus_tag="PLO58_06700"
     CDS             <5711..6043
                     /locus_tag="PLO58_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQO74514.1"
                     /translation="PAYSAAKAAVSNFTQWLAVHMAQEYSPNIRVNAIAPGFFLTDQN
                     RFLLTDKETGELTERGRKIIAATPLGRFGEPEELIGTLIYLISDSSRFVTGVIIPVDG
                     GFNAYSGV"
     gene            6079..7161
                     /locus_tag="PLO58_06705"
     CDS             6079..7161
                     /locus_tag="PLO58_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQO74515.1"
                     /translation="MNCGLNLKPTGSLDLLSLGALVHRLDPGTVPFRKATECKIHVSG
                     GEYNVAANLADCFRLNTGIATAMADYPIGDLIAERVRAMGVKPFYKHFAHNGVNGPNM
                     ATVYSDRGYGVRAPVVFYNRSNEAAALLKPGDFDWKAIFAEGVRWFHSGGIFAALSET
                     TGQVIIEAMKAAKAAGAVTSFDLNFRAKLWNLWGGEKKAVEVINEIVKYVDVLVGNEE
                     DLQKGLGIPGPEITAKSKLDPSAFIAMISDVVKKYPQIKVVATTLREVHSTNRHSWSA
                     VAWINGETYQAPTAELDVYDRVGGGDGFASGFFYGLLKNVPPEEAVKLGWAHGALVTT
                     YPGDTTMATLAEVENFIKGGSARIQR"
     gene            7183..8457
                     /locus_tag="PLO58_06710"
     CDS             7183..8457
                     /locus_tag="PLO58_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HQO74516.1"
                     /translation="MLYYAKSSADYGLTETEIRDGFFSALSKLGARRKVIAAPPDFTR
                     FHSQAGQLTRLAFQYYGKNLQAILPALGTHFPMTEAEIEAMFGDLPRHLFRIHHWKED
                     LFTLGVVPGEFVFEVSEGKLDYEWPAQVNKLLVEGGFDLILSIGQVVPHEVIGMANYN
                     KNIFVGTGGQEGINKSHFLGAVYGIERIMGRADTPVRRVLNYASDNFARHMPIVYVLT
                     VIGKNSTGQMAMKGLFIGDDYECFQLASELSLTINFDLLDKPLKKVVVYLDPQEFKST
                     WLGNKSIYRTRMAIADGGELIVLAPGLREFGEDPEIDRLIRKYGYRTTPEVMQFVKEN
                     SDLQNNLSAAAHLIHGSSENRFTVIYCPGYISREEIESVNFRYSELAPILARYDPAKM
                     QDGFNILPDGEEVFYISNPALGLWATKERFNN"
     gene            8484..9935
                     /gene="gnd"
                     /locus_tag="PLO58_06715"
     CDS             8484..9935
                     /gene="gnd"
                     /locus_tag="PLO58_06715"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HQO74517.1"
                     /translation="MNKADIGLIGLAVMGENLVLNMERHGFTVAVFNRTVEKVDKFLA
                     GRGKGKNIIGTHSIPEFIASLKKPRKIMLMVKAGQPVDDFIELLIPHLEKGDIIIDGG
                     NSHFPDTIRRTKYLEEKGFLYIGTGVSGGEEGALNGPSIMPGGSPEAWPHVKPIFQAI
                     AAKVEDGTPCCDWVGENGAGHFVKMVHNGIEYGDMQLICEAYQIMKDLLGLSYPEMHQ
                     VFADWNEGDLKSYLIEITRDILAYKDEDGQPLVEKILDTAGQKGTGKWTVVASLDAGA
                     PLTMIGEAVYARMLSAMKDERVAASKVLKGPQPEFKGDKQQFIADLGQALYAAKIVSY
                     AQGYVLMRMAAKDFGWHLNYGGIALMWRGGCIIRSVFLGKIKEAFDQNPNLSNLLLDP
                     FFKEKIESSQNAWRRVIATAVTNGIWVPALASALNYFDGFRNSRLPANLLQAQRDYFG
                     AHTYERIDKPRGEFFHTNWTGRGGETSSTTYTV"
     gene            9962..10843
                     /locus_tag="PLO58_06720"
     CDS             9962..10843
                     /locus_tag="PLO58_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQO74518.1"
                     /translation="MTLADSLKFEKKHDFLVAIDSDGCAFDTMEIKHKECFIPNIIYY
                     WNLQAVSKYARAAAEFVNLYSKWRGINRFPALTMTFDLLMDWPEVQRRGVTIPRAQSL
                     RDWIARETKLGNPALQAEVERSGDAVLQQALAWSEAVNADIARMVHDVPPFPYVRESL
                     QRLAEVADVMVCSATPTEALRREWEEHNIARYVRAIAGQEVGSKKEQIEQAASARYEP
                     QKILMIGDALGDLKAARANSARFFPINPGHEEDSWENFFKKYLDDFINDQYSEEKEGS
                     LISEFESFLPASPPWKK"
     gene            10870..11643
                     /gene="kduD"
                     /locus_tag="PLO58_06725"
     CDS             10870..11643
                     /gene="kduD"
                     /locus_tag="PLO58_06725"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HQO74519.1"
                     /translation="MENKNVLDLFKLDGKVALVTGAARGLGQAIATALASAGADVSLV
                     DILPTEETHETIAGYGRRALSLTANLMEKANIEPVVDATVKKFGRIDILVNNAGIIRR
                     APLTEFSEKDWYDVINLNQNTVFFLSQMVAKLMIARGEGGKIINIASLLSFQGGILVP
                     SYTASKSAVMGLTRAMANELAPHHINVNAIAPGYMATDNTAPLRADPARAESILERIP
                     IGRWGEPADLQGVAVFLASSASDYMTGYTVAVDGGWLAR"
BASE COUNT         3162 a         2600 c         3007 g         3304 t
ORIGIN      
        1 tgtcgaaggg tgacggctta aatcaagtgc agtattaccc ggattatcaa caacttagct
       61 tataaaacaa tgaaatattg gagaattcaa aaaaatttac ttgacaattt ggaaaaagat
      121 ttgcatttta cttaaccgat aaagttaacg gtaaagtgaa aaaatagtga acaaaccgcc
      181 aaacagatcg ccaaacgtta ccctggatga tatcgctcgc cagttaaagg tttctaaagt
      241 cactgtttct aaggcgttgc gggatcaccc tgatatcggc tcagccacca agcgcgaagt
      301 ccaggcgcta gccgctaaat tgggttacgt tcccaatttt atggcgcgta atctttcagc
      361 cagaagctca cggacgattg gtttggtcgt accgaagctg gctcactatt tttttgctac
      421 ctgcattgaa tcaatttaca atgccgccaa tgccagtcaa tacgacataa tcatgaccgt
      481 atcccaggag aatccggaat acgaactcaa acacatccag actttactgg cgatgcgagt
      541 tgaaggctta ttagtttcag tcaccgagaa tacaactgac accagcattt ttcaagatat
      601 caaaaagcgc ggtgtaccga ttgtattttt tgaccggatt ccattcggtt tggaaagtaa
      661 ttacgttgtg actgatgacc gagcggcggc ggcgcgcttg acgaattatg tcattcaagc
      721 cgggtatcgc aaaattgccc atttcgccgg ttacagtcac accaatatcg gctacgaacg
      781 tcagactggt tttcgacagg cattatctga caataatttg ccgattcgcg aagattggat
      841 tatcgaaggt ggttttggtg aacgagccgg atatgaagca ttaaaaaaag tagctgagac
      901 taaagattta cccgagttga tatttgcggt aacttttccg gttgcgctcg gcattttcga
      961 agccgctagc gaactgggtc tgaaaattcc tggcgatatt gacctagttt gttttggggg
     1021 tggtcaattt aatcgttatc tgtcgccggc attgactttt atggatcaac cagccagtgc
     1081 catcggtgag aaagccttta atctattatt ggatgaattg cattcgtcgg agagacgtag
     1141 cgaacaacat attgtcgttc catcccagtt agtaattggt gaaacctgtc gcaaattaca
     1201 atacaattat gaggagttat gattttacct tctgaccgtt tttttgattc agacccggcg
     1261 attcgatcgc tggcacgtga actttaccag caagtcaagg acttgccgat aatctcgccg
     1321 catgggcata tcgaggctaa aattttagcc gaaaatcaac cgtttccgga tccgacggct
     1381 cttttcattt tgcctgatca ttacatttat cgccttttgt attctcaagg aattccactg
     1441 gaatcgctgg gaattccgac tcgcgacggc acgccggtcg agacggatca tcgaaaaatc
     1501 tggcaaattt ttgccgataa ttattatcta tttgccggaa cgcccaccag tttgtggctg
     1561 gcttatgaat ttagtgaagt ttttggagtc accgaaaagc tcaacagcgc caatgcgatg
     1621 gcaatttatg atcatttgca aaatcaactg cagaagccgg agtttcgccc gcgcgcttta
     1681 tatgagcgat ttaatatcga ggttttgact tccactgacg gcgccactga tagcctaaaa
     1741 tatcatcgcc agataaagga atccggatgg aaaggaagaa tcgtaccctg cttccggccg
     1801 gacgctttat ttaatataaa tgcagtgggt tggcgtcaag aaatcgagaa actgggggac
     1861 gccgtcggct atgaagttaa tacttactct aaattcatta ccgctatcga aaatcggcgc
     1921 gaatttttca aaacaatggg cgcggtttcg actgatcaag gagtcgaaag cgcctatact
     1981 caccgattga gcgcgtccca ggctgaagaa atttttcaac gggcgcgtaa tggaaaagct
     2041 agcgccgatg acgcacgttt atttaccgct aatatgttaa tggagatggc tcggatgagt
     2101 gtagccgacg gtttagttat gcagatgcac gttggcgctc tgcgaaatca caatttgcga
     2161 aattttcggc gatttggacc tgataccgga gccgatattc cggtgcgttc ggagttcaca
     2221 gttaatctcc gcgaactgct gaatgaatac ggtaccgata gccgtttgac actggtggtt
     2281 tttactttgg acgagtcaac ctattcccgt gagctagcgc cactcgccgg ttattatccg
     2341 gctatgaagc tgggtgcggc ttggtggttt catgacagta ttcagggtat gattcgcttt
     2401 cgggaaatga ttacggagac cgccggtttt tataataccg tgggatttac cgatgatacg
     2461 cgatcctttc cgtcaatacc ggcgcggcac gatctttgtc gtcgggtaga ttctaatttc
     2521 ctagccggtt tggtcgttcg acacattatt gatttagatg aagcgcacgt tttaatcaga
     2581 gcgctaacct acgatttggc aaaaaaaact tataaactct aaacacatcg agtaatttta
     2641 tgagaactaa atttctaaca aaaattggac taggtaccgc aataataata agtggctttt
     2701 gcctgaatgg ttgtgagtca gaagcacgca ccaaaaccat caaattggcg catgcactgg
     2761 atacctcgca tcccgttcac aagtccttgg tttatatgag cgaacgcttt gcggaaaaat
     2821 ccggtggaaa atatcgtatt gatatttatc ccagcggtca attgggttcg gaacgcgagt
     2881 atgtagaatt gcttcagatc ggcagtctgg gaatgacaaa ggtctcgtgc agtgtgctgg
     2941 aaggttttgt ggataaaatg aaagttttca gtatgcccta tcttttccgc gacgacgagc
     3001 atcggtttaa ggttctggaa ggtgagattg gtcgcgagct tttactcgaa ggagaaaaat
     3061 actggattcg tggattatgt tatctggatg ccggctgtcg cagtttttat tcggtcaaaa
     3121 agccgattaa tactccggag gatttacgag gtttaaagat tcgaacacag gaaagcacga
     3181 cttccattca aatgattcag gctatgggcg gttcggctac gccgatcgcc tggggcgaac
     3241 tttatacggc tctgcaacaa ggtattgtgg atgctgcgga aaataatcct ccctcctttt
     3301 acctatcgca tcatcacgaa gtagctaaat attttgcgct ggacgaacat actgccgtgc
     3361 cggatatatt attgattagc acgaaggtct ggaatgatct accacctcaa gttcaggagt
     3421 ggttgcagtc tgcggcggac gaagccgcta tataccagcg tgaactgtgg aaaattgcca
     3481 cgcaggaagc gctggaggaa gtccaaaaag cgggcgtggt tgtgagttat cctgacaaac
     3541 gtccatttca agaaaaagtc aaggacattt acgaaagtta tcgaagtgat cctgagattt
     3601 atgaattaat ccaaagaatc agggcggtgc aatgatgtta aaaatacgca aaatactcga
     3661 taatatttta aaatgggtcg tagtggtttt aatggctatc tcggtgttta atgtgctctg
     3721 gcaggtattt acacgcttta tccttcgtaa tccgagttcc tataccgaag aactggcgcg
     3781 ctatctgcta atttgggttg gcttactcgg cgccgcatat gccgccggaa ataaaatgca
     3841 tctggcgatt gatttattca gtgccaagat gaaaagaaat aacgcactcc gcttgcagat
     3901 attcatccag atttgtgttc tgttttttgc gatctccgta atggtaattg gcgggttgcg
     3961 cctagtaact attaccttga ctttaaacca attatcggcg gctttgaagg taaaactggg
     4021 ctatgtttat atggttttac ctattagtgg gatattaatt gccatttact caattgtgga
     4081 aataattggt ttatttggcc agctatttcg acccgattca acaaataatc tacctgaaaa
     4141 aaggacggaa taccatggag tggactgaat taatagtact agttataagt ttcgtttttc
     4201 tgctattagc cggagtacca atttccttca gtattggtat cgctactctg agcactatgt
     4261 tgctcagtat tgatactctg ccggctctga cgaccgttgc ccaacgaatg gcgaccggta
     4321 ttgatagttt tgccttgctg gcaatcccgt tctttatctt atctggctta ttaatgagtc
     4381 ggggtgggat tgcgcggcgc ttgattgatt ttgccaaggt gttggtggga cgctggccgg
     4441 gtgggctggc ttatgtcaat atcttggcgg cgatgttttt tggagcgatt tccggttcag
     4501 cggcggcggc ggcggcggca attggcggtt ttatgacccc actgatggaa aaagagggct
     4561 acgacaccaa ttattccgca tcggttaata taacctcagc tacgactgga ttattgattc
     4621 ctcccagtaa tatcctgatc gtatatagtc ttgcgagtgg cggggtttcc attgcggctc
     4681 tttttatcgc cggctacctg ccggggattc tggttggctt attgctaatg gtagcagctg
     4741 gtattattgc cgtgaaaaaa ggatatcggg gaagcgacga gcgctatact ttcaaacaag
     4801 gtattatgcg ctttctggat gctctaccaa gcttgatgct gattgtcatt gtcatcggcg
     4861 gaattattgc cggatttttt accgcaaccg aaggttcagc gatcgcagtg ctttatacct
     4921 ttatcctatc ggtattaatt tatcatgaag tcaaatggaa ggagctaccg caggtactgc
     4981 tggaaacggt cacgacgacg gctattgtta tgctgttgat tggaacttcg atggctatgt
     5041 cgtggattat gtcttacgcc aatattcctc agaccatcac cgcttcgtta attgccttat
     5101 ccagcagtaa aatcatcatt ctgctgataa tcaacttgat tctgctgttt gtgggaacat
     5161 ttatggatat gacgccggcg gtattgattt ttacaccaat attcctaccg attgtaaccg
     5221 ctttgggcat tcatccgatt cactttggaa taatcatggt tctgaacttg tgcattggat
     5281 tatgcacgcc accggttgga tcggttctat ttatcggatg tgggattgcc aataccacaa
     5341 ttcccaaaat agtaaagtca attctaccat tttatgcggc tatgatttta gctttgttat
     5401 tggtgacata ttttccgcag attagtctct ttttaccacg tttatttgga tattaattta
     5461 aaaggagaac gagttaatga aaaccgattt aaatgacaaa accgtggtca tcaccggtgg
     5521 gtccggcatt ttaggacgtg tgatggtttc cgagctattt aaagccggag cgaatgtagc
     5581 ggtactcgat ttgcgtgaac cgccgacaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn ccggcttatt cagccgccaa agccgccgtt agcaatttta cgcaatggtt
     5761 ggcggttcac atggcgcagg aatattcccc aaatatccga gtcaatgcaa tagcgccggg
     5821 ctttttcctc accgaccaga atcgcttttt gttgacggac aaggagaccg gtgaattgac
     5881 cgaacgtggg cgtaaaatta tcgccgctac gccattgggg cgctttggtg aaccggaaga
     5941 attaattggg acgctgattt acttgatttc agacagttcc agattcgtga ccggtgtgat
     6001 tattccagtg gatggcggtt ttaatgccta cagcggggtt tgaattattt aataactaaa
     6061 aatgagagga tttgtaatat gaactgtgga ctaaatctta aaccgactgg cagtcttgac
     6121 ctgctttcac tcggggcgtt ggtgcatcgg ttggatccgg gcacggtgcc gttccgcaaa
     6181 gctaccgaat gcaaaattca cgtcagcggt ggtgaatata atgtggcggc taacctggca
     6241 gattgctttc gactgaatac cggcattgcg accgctatgg ctgattatcc gatcggcgat
     6301 ttgattgctg agcgtgtgcg ggcgatgggg gtcaagccct tttacaaaca ttttgcccat
     6361 aatggggtga acggtccgaa tatggcaacc gtttacagtg atcgcggcta cggcgtacgc
     6421 gcgccggtgg ttttctacaa tcgttcgaac gaagccgccg ctttgttaaa acctggtgat
     6481 tttgactgga aggcgatttt cgccgaaggt gttcgctggt ttcacagcgg tggcattttt
     6541 gcggcgctct cggaaactac cgggcaggta atcatcgagg ctatgaaagc cgctaaggcg
     6601 gctggtgcgg tcactagctt cgacttgaat tttcgcgcca aactctggaa tttgtggggc
     6661 ggtgaaaaga aagcggtgga agtcattaat gaaattgtca agtatgtgga tgtgctggtc
     6721 ggcaacgaag aggatttaca gaaaggtctg gggattcccg gcccggaaat aactgccaag
     6781 tcaaagctcg atccatcggc ttttattgct atgattagcg atgtggtcaa aaaatatccc
     6841 cagattaagg ttgtggcaac taccctgcgg gaagttcatt cgactaatcg ccacagctgg
     6901 agcgcggttg cctggatcaa tggcgaaact taccaagcgc cgacggctga attggatgtt
     6961 tatgatcggg ttggcggcgg cgacggcttt gcgtccggtt ttttctatgg tttattgaag
     7021 aatgttccgc ccgaagaagc cgtcaaactc ggctgggcac acggagctct cgtaacgact
     7081 tatccgggcg atacgacgat ggcaacttta gccgaagtag aaaattttat taaaggcggc
     7141 tcagcccgca tccagcgcta agccgtttta ggacaatcca taatgttgta ttacgctaaa
     7201 agttcagctg attacggact gacggaaacc gagattcggg acggcttttt ttcagctctg
     7261 tcaaaattgg gagcgcgccg taaagtgata gccgctccgc cggattttac tcgttttcat
     7321 tctcaggctg gccaactgac gcgtttagca tttcaatatt atggcaagaa tctacaggcg
     7381 attttgccgg cgctcggcac acattttccg atgaccgaag cggaaatcga ggcgatgttc
     7441 ggtgatttgc cgcgccacct tttccgcatt catcactgga aagaagacct atttaccctc
     7501 ggcgtggtgc ccggtgaatt cgtattcgaa gtctccgaag gaaaactgga ttatgaatgg
     7561 ccagcgcagg ttaataaact attagtcgag ggcggcttcg atctgatttt atccatcggg
     7621 caagttgtgc cacatgaagt gatcggtatg gcaaattata ataagaacat tttcgttgga
     7681 accggcggtc aggaaggtat aaataagagc cattttttag gtgcggttta cggaatagaa
     7741 cgcattatgg gacgcgccga tacgccggtg cgccgcgtgc tgaactatgc ttcagataat
     7801 tttgcccggc atatgccaat cgtctatgtc ttgacggtaa ttggaaaaaa tagcactggg
     7861 caaatggcta tgaaaggctt atttatcggc gacgattatg agtgtttcca gctggcgagc
     7921 gaactgtcgc tgactattaa cttcgatctg ttggataaac cgctcaagaa agtcgtcgtt
     7981 tatctcgatc cgcaggaatt caagagcact tggctcggta ataaaagcat ttatcgaact
     8041 cgtatggcga ttgccgacgg tggtgaattg atcgtgctgg cgcccggttt acgtgaattt
     8101 ggtgaggatc cggaaattga tcgactgatt cgtaaatacg gctaccgcac tacgccggaa
     8161 gttatgcaat tcgtaaagga aaatagtgac ttacagaata atctcagtgc ggccgcccat
     8221 ctgattcacg gttcgtcgga aaaccgcttt acggtaatat attgccccgg ttatattagt
     8281 cgggaagaaa tcgaaagcgt caatttccga tatagcgaat tggcaccaat cttggcacgt
     8341 tacgatccgg cgaaaatgca ggatggtttc aacattctac cggacggcga agaggtattt
     8401 tatatctcga atccggcgtt agggctctgg gctaccaagg aaagatttaa taattgaatt
     8461 ataatatcag gaggatttaa aaaatgaata aagcggacat aggtttaatt ggattagccg
     8521 ttatgggcga gaatttggtg ctgaatatgg agcggcatgg tttcacggtg gcggttttca
     8581 atcgcaccgt cgaaaaagtg gacaaatttc tggccgggcg tggcaagggc aaaaatatca
     8641 tcggcactca ctcgattcca gaatttattg cctcgttaaa aaaaccacgc aagattatgc
     8701 tgatggtaaa agccggtcaa ccggtggatg attttatcga attactaatc ccgcatttag
     8761 aaaaaggcga tataattatt gacggcggca attcgcattt tccggataca attcgccgca
     8821 caaaatatct ggaggagaag ggttttctgt atatcggcac cggggtctcc ggcggagaag
     8881 aaggcgcgtt aaatggtccg tcaattatgc cgggtggttc accggaggct tggccgcatg
     8941 tcaagccgat ttttcaggcg attgcggcta aagtcgagga cggcacaccg tgttgcgact
     9001 gggttggtga aaacggcgct ggtcattttg tcaaaatggt tcacaacggc atcgaatacg
     9061 gtgatatgca attgatttgc gaagcctacc aaattatgaa agacctgctc ggcttgagtt
     9121 accccgaaat gcatcaggtt ttcgccgatt ggaatgaggg tgatctaaaa agctacctga
     9181 ttgagattac ccgcgatatt ctggcttata aagatgaaga cggacagcct ctggtggaga
     9241 aaattctgga taccgccggc caaaaaggta ctggcaaatg gacagtggtg gcttcactgg
     9301 atgccggcgc accgctaacg atgattggtg aagccgttta tgcccggatg ctttcggcga
     9361 tgaaagatga gcgcgtcgcg gcttctaaag ttttgaaagg accgcaaccg gaattcaaag
     9421 gtgacaagca acaattcatc gccgacctcg gtcaagcctt gtatgcggct aaaatcgttt
     9481 cctatgccca aggctacgtt ctgatgcgaa tggcggctaa ggacttcggc tggcatttaa
     9541 attacggcgg tatcgcctta atgtggcgcg gcggctgtat cattcgttcc gtatttctgg
     9601 gaaaaatcaa agaggcgttc gatcaaaatc ccaacctgag caatctactt ctcgacccat
     9661 ttttcaagga aaaaatcgaa tcgtcgcaaa acgcttggcg tcgggtaatt gctaccgccg
     9721 ttactaatgg catctgggtt ccagcgctgg cttccgcttt gaattacttc gatggattcc
     9781 gcaacagtcg cctgccggct aatcttctgc aagctcagcg tgattatttc ggcgcacata
     9841 cttacgagcg gattgacaag ccgcgtggag aattctttca tactaactgg accggacgtg
     9901 gtggagagac atcttccacg acttatacgg tctgattcat ttccacgaag gagacagaac
     9961 gatgacgctg gcagattcgc taaaatttga gaaaaagcac gactttctgg tcgcgataga
    10021 ttcggacggc tgtgctttcg atacgatgga aatcaaacac aaggaatgct ttattcccaa
    10081 tattatctat tattggaatt tacaagccgt ttcgaaatat gcccgcgccg cagctgaatt
    10141 cgtcaatctc tattccaaat ggcgggggat caaccgcttt ccggctttga cgatgacttt
    10201 tgacttatta atggattggc cggaagtgca acggcggggc gtaaccattc ccagagctca
    10261 gtccttgcga gattggattg cccgcgaaac caaactcggc aatccggctc tccaagccga
    10321 agtcgaacgg agcggcgatg cggttttgca acaagctctg gcttggagcg aagccgtcaa
    10381 tgccgatatc gctcgaatgg ttcacgatgt accgccgttt ccttatgtaa gagaatcatt
    10441 acagcgtctt gccgaagtag ctgatgtaat ggtttgctca gcgacaccca cggaagctct
    10501 gcggcgcgaa tgggaagaac ataacattgc gcgctatgtt cgggcgattg ccgggcagga
    10561 agtcggcagt aaaaaggagc agatcgagca ggcggcaagc gcaagatatg aaccccagaa
    10621 aattctaatg attggcgacg ctctcggcga tctcaaagcc gcccgcgcta attcagctcg
    10681 ttttttccca ataaatccgg gacacgagga agattcctgg gagaattttt tcaaaaaata
    10741 cctggatgat ttcatcaacg accaatattc agaagaaaag gaaggttctc tgattagtga
    10801 atttgagtcg tttctaccgg cgtctccgcc gtggaagaaa taaatttcaa gacaattaaa
    10861 gaggagtata tggaaaacaa aaatgtttta gatttattca aactggacgg taaagtcgcg
    10921 ctggttaccg gtgccgcgcg gggacttggg caggcgattg ccacggctct ggcttcagct
    10981 ggagccgacg tcagcttggt agacatttta ccaactgaag aaactcacga aactattgct
    11041 ggctatggtc gccgagcgct cagccttacc gctaatttga tggaaaaagc caatattgag
    11101 ccggtagttg acgccacagt gaaaaaattc ggccgcattg atattctggt caataatgcc
    11161 ggaattattc gacgcgcacc gctgacggaa ttttccgaaa aggactggta cgatgtcatt
    11221 aaccttaatc agaatacggt ctttttcctc agtcagatgg tggctaaatt gatgattgcg
    11281 cgaggagaag gcggtaagat tatcaatatc gcttcactat tgtcctttca gggcggaatt
    11341 ctggtgccgt cctataccgc ttcgaaaagc gccgttatgg gattgacccg cgcaatggct
    11401 aatgaactcg cgccgcatca tatcaatgtc aatgccatcg cacccggtta tatggcgacc
    11461 gataacaccg cgcccctgcg cgctgatccg gcgagagcag aatccattct tgagcgtatt
    11521 cctatcgggc gctggggcga accggctgac ttacaaggcg tggcggtttt cttagcctcg
    11581 tcagcatccg attatatgac cggctatacc gtggcagttg acggcggctg gctggcaaga
    11641 taactaattg ggaactaaat gaccatttaa ttttatgctg atgtaacgtc ccagagtaga
    11701 acgacattaa tatcaatccc acttttcctg tcggtttgac ctgcattcct tagataattt
    11761 ggaatattgc aaagctgtcc agctgatatg aaatccggtt ttggatatac aagttttgaa
    11821 ttttctataa catagattta ccgcatcaag aacagaaaaa aaccagattc tttaggacag
    11881 tactatgaaa cgggttgaaa tttaattaca atcgccgcct tgattgaccc gaacgatttc
    11941 caccagaaaa atagttagat cacaacggta tttgcactat ttctaatccg atagcatata
    12001 aacaagtgat atgctcccga acgcgagaac catcccgctt catcactcaa ttaggttcta
    12061 ttgccagtta cctaactgtt gattcttcga ctcaattaag ctcttttcta aaacctttta
    12121 atcaaatcta atactaataa caccagtcaa atcgaatttg cttctcgaat aat
//
LOCUS       DAOMJU010000061        12063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5360_length_12063_cov_24.945370, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000061 DAOMJU010000000
VERSION     DAOMJU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12063
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5360_length_12063_cov_24.945370"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3842
                     /locus_tag="PLO58_06730"
     CDS             <1..3842
                     /locus_tag="PLO58_06730"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HQO74520.1"
                     /translation="IKPKNKGKEFLYLILMCFQVNGRYWNSVRINFQQSLLKITRLGV
                     IISLLIMLIGLVFFDSADAAEKYEPLRLIRADKLEQFYRADTPIKRLTGNVLFQKGGL
                     QLACDLAYWFERDQRADFYRNVIATSDNKVLKADSLVYFSENDSIVARGHPILYDSTL
                     SITARRLIYLVKDEIAHAEGKVLLKDSSRSVRANLIHYYSNEKKSVAIGQAVLYDVKR
                     HLELASDSLIYFNKTGNIFALNRPMLTRFDTAGVESFRIYADTIRLVEKEGNFQAIGN
                     VRIQSKDFTAYSQTLTYIDSLEVATLTDNPRVLSDGQELTGKVMRLHFENEKISALYI
                     DEDAHANTVGKAYLPAESDSLAHGDSIKTYDEITGKFMEIYFRDDQADSIVVAVMATS
                     LYNMLEDSIILGINEVSGDTIRLKFIDRKINTITVVGGTQGRFIPHETNTDLDTTVIY
                     SAERIDYFVDRKLTDLYLNARIQYADIELTAGKININWNENLLYAYPLSPPPYDSTSV
                     DLPVLNQRGREPFVGESMVYNLKTQRGRIVSGKTKEQDGYYYGDKISKVDKKVFYVND
                     CVYTTCDIPDKPHYYFRSKQLKIIFKDKVIARPVIFYIHDIPLLGLPFIIIPDQSGKR
                     HSGWIMPSYGESRIGGGFIRGLGYFWAPNDYYDFRLTGDFYDKQGVILDYRLRYAWRY
                     KLNGTISGKYTNDFFSNYPEKQWAFNITHNQTINPTTRLNVNGKFVSNESLYKKYSYD
                     LEDRLDQQLISNATLSKSWTGKPYSLSVNFNQTTNLQANALSQIEPTDSNLTIAHISR
                     SLPNISFTRSSKPLIPLKPHQDVSRSKWYNNIYFSASSYLKNNQNIFYQSIYNPATDS
                     LEWQEQNIAKNAVTYNFSFTSSQKIFGFLTTNQTVNLDEGWVFKYDMPLYDEDHRFQL
                     ENGKIKTETVEGFKARHTGSATISAQTKAYGLFPVRIAGLQAVRHVVTPSVSMTFRPD
                     FSREIFGWDPGYIIGGIDTTGTARTFDPLTNTMLGALSSYETRTMSMSLSNLFQAKLG
                     QGEKTRKLDLFTLNASTGYNFAADSLNWSVISSSFRTQVTKKIALNLNATHDLYAYQN
                     NRRVNEWNKTRNGIPIPRLTTVSASTGFSLSGKRFGSPQQNETIPTSDISSDTTDINI
                     ITPSYGETDINKLQESAPRLGSELWTANFNLRYSLSQTNPHKRTETLFMTTNLTLNLT
                     SKWKVVYQNSMNLIDKKIVSQSVSVTRDLHCWQLAFSWTPSGYGKQYLLLINIKSPTL
                     RDIKYEERGGRRSSLGF"
     gene            3883..4677
                     /locus_tag="PLO58_06735"
     CDS             3883..4677
                     /locus_tag="PLO58_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQO74521.1"
                     /translation="MSQISNAKSLFNPAYDILALDFDGVIVDSIAECLVVGHNAFQSA
                     IGRSEKINDLSQLPREIQQESRRLRNFIRHGEDYVYIFKAIADDFSIHNQNDFDDYLE
                     KNIDVKEKFRTQFYLERARFLREEPLRWLALNPFYDGMADFLKNFSPPEHLYIITTKK
                     LEYVQVILDTAGIPLQRKNLFAANSQKGKPEIIADLLQKTRLAPDSFYFIDDQVDTLI
                     KLKPLKIRLFVAGWGYTNQEQIRQAETEGIPTLTLNEFYQLFSRLD"
     gene            5017..6027
                     /gene="gap"
                     /locus_tag="PLO58_06740"
     CDS             5017..6027
                     /gene="gap"
                     /locus_tag="PLO58_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQO74522.1"
                     /translation="MAIKVAINGFGRIGRLVLKASYKNPQVDIVAINDLTDAKTLAHL
                     LKYDSVHGKFDGTVSVDGNYLVINGKKIEVFAQKDPSLLPWGKLGVEIVVEATGKFRN
                     REGMQKHITAGAKKVILTVPADSKEDVDVTIVPGVNDEMLTKNSIFVSNASCTTNCLA
                     PVAKVLNDNFGIKRGLMNTIHSYTNDQVILDFPHKDLRRARAAAVSIIPTKTGAAKAI
                     GLVIPALDKKMDGFAMRVPTPDGSVVDLTCELEKPATKEEINAAMKAAAEGPMKGILE
                     YTEDPIVSIDIVGNPHSSIFDAGMTKVLDGNFAKIISWYDNEWGYSCRVAELIVKMGL
                     LK"
     gene            6049..8169
                     /locus_tag="PLO58_06745"
     CDS             6049..8169
                     /locus_tag="PLO58_06745"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HQO74523.1"
                     /translation="MKTVNFIFGIHNHQPVGNFDFVMESAYRQSYLPFIEIVEEFSSI
                     HITFHFSGCLLEWIEAHHPDYLDRIAALVKRGNVEIISGGFFEPVLAMIPDADKVGQI
                     KMMNDYIQQRFNYTAKGLWLTERVWEPHLAKPIAEAGIKYITVDDYHFLSNGKQESEL
                     TGHFLTEEQGKTLGIFPISQRLRYTIPFQDPQATIDHLAKFATDDGENVIVMADDGEK
                     FGVWPGTHEQVFTKGWLRNFFAALLKNSEWLKTLTFAEWYENHPPKGRIYLPTASYFE
                     MSEWALPWEAGEKFADLVHEFESKKRIEEVRPFIKGGIWRNFLYKYDESNWMQKKMLH
                     LSERFADIDPTSLTKPQQVELGQARLHLWRSTCNCAYWHGIFGGLYLPHLRHAIYTEL
                     LICERLLDKITGAKGFRAEVFDFDTDGENEIIIKYGNLHTAIAPHRGGMITELSLVNK
                     AFNLLNNIQRYHESYHRKVALADKVENSNASGSIHDLVLSKEKGLDKYLKYDKYPRKN
                     LMDHVIHPSVSLERFRDGDYYEDSDFITGHYSPELNEKTRTLTLSKDGWVNWQRFRIK
                     KTITFGKEALKVTYILTNIGERENVFRFGPEFNFALLGGNSPDRYYIADGEKTAEPAL
                     NSIGILTGIKSLGVVNEWDKFQAEVIAPQAVEFWRFPIETVSLSEAGFERIYQSSVII
                     PWFDVRLNPEETATFSIELTTTEL"
     gene            8589..9533
                     /locus_tag="PLO58_06750"
     CDS             8589..9533
                     /locus_tag="PLO58_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HQO74524.1"
                     /translation="MKKHPTKLVKIDPIPILLERAPLPIRYFTAERFLSHDNELVEQL
                     RRDLQFFKPRERLLATQQEDGLWKLNKNYKIEEQQKSMQFLLQLQNMMQLLDYGCTKD
                     MPAIQRGLIAILKYQKPDGKFPLLQHHQGLTLWLLVRYGLAGNPFVEKGFRWLAKRQR
                     EDGGWLSPTMLATGESAKTARSGIWTTLVITQAFSAHSRLRNTETCQRAANFLLNVYL
                     QQNHTTLFPEPDAWNHLYIDYSENGLFRGGTLRFLETLTPLPNCHSHPNFKKALDWLI
                     GQQLPSGLWPAIAGRSKEGDYAVTFRVMVVLSEIDRIG"
     gene            9567..11939
                     /gene="lon"
                     /locus_tag="PLO58_06755"
     CDS             9567..11939
                     /gene="lon"
                     /locus_tag="PLO58_06755"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQO74525.1"
                     /translation="MAETIKNKDEKNETGHGVPEILPIMALRNTVLFPQQVIPIYIGR
                     ERSLRLIEDLPETKKRIVVVAQKEGTLENPEVDDLYEWGTIAQVLKIFNMPDGSKSAI
                     VQGLERAKIQSFIQVEPYFKGAILKAQEIYQPGIEIDAMLANLRQLYQKLIKIAPYLS
                     EEQASVFSSVQHPGRLIDRIIHLLNVPVSEKQEILEELDVRKRLEKATVILNREIQRI
                     QLGEQIQSEVQDEISKTQREYFLREQLKAIRKELGEDEGTLELKELEEKINLSKMSDE
                     ARKVAEKELDRLRKIPPASPEYTVSRTYLDWLVDLPWGIYTTDNVDIKSAQKILDEDH
                     YGLEKIKDRIIEYLAVRKLKQEQDPNASIKGPILCFVGPPGVGKTSMGKSIARAMGRK
                     FVRISLGGVRDEAEIRGHRRTYIGALPGRILQSIRKAESSNPIFMLDEIDKIGADFRG
                     DPSSALLEVLDPEQNSTFSDHYLEVTYDLSKTMFIATANMVDTILPALRDRMEILEFS
                     GYIEEEKLNIAKTFLIPKQIKEHGLTNVTVEFTDKAIRFIINSYTREAGVRNLEREIA
                     NVCRKIAREVAVNKRKKNFLIDEKAVENFLGPQRYFVDIAERLSKPGVAIGLAWTSVG
                     GDILFVEATSMRGTGKLNLTGQLGDVMKESAEAALSYIRSNATLLGLDEEAFSKIDIH
                     IHVPAGAIPKDGPSAGVTILTAVYSLLKKRKVKDNLAMTGEITLRGAVLPIGGVKEKV
                     LAAHRAGLRRVILPAQNRKDLIEIPKQIKNEMRFDFVKEMSEVLNLAIRK"
     gene            11989..12062
                     /locus_tag="PLO58_06760"
     tRNA            11989..12062
                     /locus_tag="PLO58_06760"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12023..12025,aa:Val,seq:tac)
BASE COUNT         3574 a         2628 c         2617 g         3244 t
ORIGIN      
        1 taattaaacc taagaacaaa ggaaaagaat ttttatattt aatattgatg tgttttcagg
       61 taaatggtag atattggaat agtgtaagga ttaatttcca gcaatcactc ttgaaaatta
      121 ctcgactggg tgtaataatt agtttgctaa ttatgctgat tggattggtg ttctttgatt
      181 cggccgacgc cgccgaaaag tatgagcctc tgcgtctcat tcgcgccgac aagctcgagc
      241 agttttatcg cgccgataca ccgattaaaa gactgaccgg caatgtcctt tttcagaaag
      301 gtggattgca attggcttgc gatctggctt attggttcga aagggatcag cgcgctgatt
      361 tctatcgcaa cgtcatcgcg accagtgata ataaagtcct gaaggctgat tcgctggttt
      421 atttcagcga aaatgattcg atcgtcgccc gtggccaccc gattctatat gacagcactt
      481 tgtcaataac agcccgtcgc ttgatatatt tagtcaaaga cgaaatcgct catgccgagg
      541 ggaaggttct actcaaggac agcagtcgga gcgttcgtgc taacctgatt cactattatt
      601 ctaacgaaaa gaaatcagtc gccatcgggc aggctgtttt atacgatgtc aagcgacacc
      661 tggagctggc ttccgacagt ttgatttatt tcaataagac tggcaatatt tttgccttga
      721 accgaccgat gctaacccgt tttgatactg ctggtgtcga atcgtttcgg atttatgccg
      781 ataccattcg cttggttgaa aaagaaggta attttcaggc aattggcaac gtgcgcattc
      841 agagtaagga ctttaccgct tattctcaaa ctctgacgta cattgattcg ctggaagttg
      901 ccacgctgac cgataatcca cgggtgttga gcgacggcca ggaattgacc ggcaaagtta
      961 tgcgcttaca ctttgagaat gaaaaaattt cagcgttgta tattgacgaa gatgcacatg
     1021 ccaataccgt gggcaaggct tatttgccag ccgagtccga tagtctcgct catggcgatt
     1081 ctattaaaac ttatgacgaa atcactggca agttcatgga aatttatttt cgagacgatc
     1141 aagccgattc gatcgtggtt gccgttatgg cgaccagcct gtataacatg ctggaggaca
     1201 gtatcattct gggcattaat gaagtcagcg gcgacacaat ccggttaaaa ttcattgacc
     1261 ggaaaatcaa tacaatcacc gttgtcggcg gcacgcaggg acgctttatt ccgcacgaaa
     1321 ccaacaccga cttggatacc accgttatct atagcgccga gcggatagac tattttgttg
     1381 atcgcaaact gaccgatctt tatctaaacg ccagaattca gtatgccgat atcgaattga
     1441 ccgccggcaa gattaatatc aattggaatg aaaatttatt gtatgcatat ccacttagtc
     1501 ccccgcctta cgattccacc agcgttgatt tgccggtcct aaaccagcgc gggcgagaac
     1561 catttgtcgg cgaaagtatg gtttataatt taaaaactca gcgcggtcgc atcgttagtg
     1621 gcaaaaccaa ggaacaggac ggttattatt atggcgataa aatcagtaaa gtagataaaa
     1681 aagtctttta cgtcaatgat tgcgtttaca cgacttgtga tattccggat aagccacatt
     1741 attatttccg cagtaaacaa ctaaaaatta tctttaaaga taaagtgatc gctcggccgg
     1801 taatttttta tattcatgat ataccgcttt tgggattgcc gtttattatt attccggatc
     1861 agagcggcaa gcgtcattct ggctggatta tgccctccta tggcgaaagc cgaatcggcg
     1921 gtggattcat cagaggactg ggctattttt gggcgccgaa cgattattac gatttccgtc
     1981 tgactggtga tttttacgat aaacagggcg ttattctcga ctatcgccta cgctatgcct
     2041 ggcgttataa actgaatggc accatcagcg gcaaatacac caatgacttt ttcagcaact
     2101 atcctgagaa gcaatgggct ttcaatatta ctcacaatca aacgatcaat ccaaccactc
     2161 gcctaaacgt caacggcaaa tttgtcagca atgaaagtct atataaaaaa tacagttatg
     2221 atctggaaga ccgcctcgac cagcaattga ttagcaatgc cacactctcc aaatcatgga
     2281 cggggaaacc atattcattg tcagttaatt ttaatcaaac cactaacctg caagccaatg
     2341 ctctcagtca aatcgaaccg accgattcca acttgaccat tgcgcacatt agtcgttcat
     2401 tgccgaatat ttctttcacc cgaagcagta agcctttgat tcccctcaag ccgcatcagg
     2461 atgtttctcg atctaaatgg tataataata tttacttcag cgccagctcc tatctgaaaa
     2521 ataaccagaa tattttttac cagagcattt ataatcctgc taccgattca ctggaatggc
     2581 aggagcagaa catcgcaaaa aatgccgtta cgtataactt ctcattcacc agttcccaga
     2641 aaattttcgg ctttttgact actaatcaaa ctgtcaacct cgatgaaggc tgggttttca
     2701 agtacgatat gccgctttat gacgaagacc accgctttca gctggaaaac ggtaaaataa
     2761 aaaccgaaac cgtagaaggg tttaaagccc gccataccgg tagcgccaca atcagtgccc
     2821 aaaccaaagc ttatggcctg tttcccgtca ggattgccgg tttgcaggct gttcgccatg
     2881 tcgttacgcc atcggtcagt atgacattta ggcctgactt cagccgagaa atttttgggt
     2941 gggatccggg ctatatcatt ggcggaattg acacaaccgg caccgcccgg actttcgacc
     3001 cactgactaa tactatgctg ggcgccttgt cttcttatga aacccgcaca atgtctatga
     3061 gtctcagtaa tcttttccag gctaaactgg gacaaggcga aaaaactcgc aagctcgatc
     3121 tttttactct gaatgcttcg accggctata attttgccgc cgatagtcta aactggtcag
     3181 tgatttcgag ctccttccga actcaggtta ccaagaaaat agctctcaac ctgaacgcaa
     3241 cccacgacct ctatgcttac caaaacaacc gtcgcgtcaa tgaatggaat aagacccgga
     3301 atggcattcc aataccgcgt ttgacgactg tcagcgcttc gaccggcttt tcactttccg
     3361 gtaaacgttt tggaagtcct cagcaaaatg aaacaatacc cacttccgac atttcatcgg
     3421 atacaactga cataaatata attaccccat cttacggtga aacggatatc aataaattgc
     3481 aagaaagtgc gcccaggctg ggaagtgagc tctggaccgc caatttcaat ttgcgttact
     3541 ctttgagcca gactaatcca cataaacgca ccgaaactct ctttatgact actaatctca
     3601 ctctgaattt gacctctaaa tggaaagtag tctatcaaaa cagtatgaat ctaatagata
     3661 aaaaaatcgt cagtcagagc gtctcggtaa ctcgtgatct gcactgctgg cagttggctt
     3721 ttagctggac accgtccggt tatggtaaac aataccttct cctaatcaat attaaatcac
     3781 caaccctccg cgatatcaaa tatgaagagc gcggtgggcg ccgcagtagc ctgggtttct
     3841 aaaaagaata tgaaattata attgaaccaa agtaactaaa aaatgtcaca aatttccaac
     3901 gctaaatctc tttttaatcc agcttatgat attctggcgc tggatttcga cggcgtcatc
     3961 gttgatagca tcgcggaatg tttagtcgtc ggacacaatg ccttccaaag tgccatcggc
     4021 cgttcggaaa agatcaacga tctaagtcaa ttgccgcgcg aaatccaaca agagagtcgc
     4081 cgcttacgca attttattcg gcacggtgag gattatgtct atatatttaa agcaattgcg
     4141 gatgactttt ccattcataa tcagaatgat ttcgatgact atctcgaaaa aaatattgat
     4201 gtcaaagaaa aattcagaac gcagttttac ctcgaacgcg ctcgctttct gcgcgaggaa
     4261 ccactgcgct ggctggctct taatccgttc tatgacggaa tggcggattt cctcaaaaat
     4321 ttctcaccgc cggaacattt atacataatt actaccaaga aactcgaata tgttcaggta
     4381 attttagaca cggccggcat tcctcttcaa cggaaaaatt tattcgcggc taatagccaa
     4441 aaaggtaaac cagaaataat tgccgatctt ttacaaaaaa cgagattagc accagatagt
     4501 ttttacttta ttgacgacca ggttgatact ttaatcaaac tcaagcccct aaaaattcgc
     4561 ctgtttgtag ccggttgggg atatacgaat caggaacaaa tccgccaggc tgaaactgag
     4621 ggcattccga ccttgacttt gaatgaattt tatcagctgt tttccagatt ggattgacat
     4681 aattttcggt catttctctt gtttttaaat tttgacggct aaatgattcg attgacagtt
     4741 ctataagaat gaaaataagg cgctttttga aaagtaataa atcagaaaac aagttattgg
     4801 caaaaaaata ttgatgagga aatggaaaaa taggctgggg tattagtgat ttcccgagaa
     4861 ttggcggaat ttctaaagaa ttaaagtcca tttcccacaa tttttttgga agtaaattgc
     4921 tggaaaatta aatttcaggt cggctttttg aaaattaata atcattttta aaaatcaccc
     4981 gaacaaattg aaaaataaaa caaaaggagt ataattatgg caattaaagt cgcaatcaac
     5041 ggatttggtc gcatcggacg tctcgtctta aaggcttcct acaagaatcc tcaggttgat
     5101 atcgtcgcaa tcaatgattt gaccgatgct aagacattag cccatttgct aaaatacgac
     5161 agcgttcatg gcaaattcga tggtaccgtc agcgttgacg gcaattattt agttattaac
     5221 ggaaaaaaga ttgaggtttt cgcacagaaa gatccctcct tattgccgtg gggtaaattg
     5281 ggtgtggaaa ttgtcgtcga agccaccggc aaattccgca atcgtgaagg catgcaaaaa
     5341 cacatcacgg ccggtgccaa gaaagtgatc ctgactgttc cagctgactc caaggaagat
     5401 gtggacgtaa ctatcgttcc aggtgtcaat gacgaaatgc tgacgaaaaa ttcaatattc
     5461 gtctcgaatg cttcttgcac gactaattgc cttgctccgg ttgctaaagt tctgaatgac
     5521 aatttcggca tcaagcgcgg tttaatgaat acaattcatt cttacacgaa tgatcaggtc
     5581 attcttgatt ttccgcataa agacctgcgt cgggcgcgcg ccgcagcggt gtcaattatc
     5641 ccgaccaaaa ccggcgcggc caaagctatc ggcctcgtta ttccggctct tgacaaaaag
     5701 atggacggct tcgctatgcg cgttccgact cccgatggtt ccgttgttga tttgacttgc
     5761 gaactggaaa aaccagccac caaagaagaa atcaacgccg ctatgaaagc cgccgctgaa
     5821 ggcccaatga aaggcattct cgaatacact gaagatccta tcgttagcat tgacattgtc
     5881 ggtaatcccc actcttcaat tttcgacgcc ggaatgacta aagttcttga cggcaatttc
     5941 gctaagatca tctcctggta cgataacgaa tggggttaca gctgtcgggt ggccgaatta
     6001 attgtaaaaa tgggcctttt aaaataaagc tgtctcaata gcatacattt gaaaaccgta
     6061 aattttatct tcggcattca taaccatcag ccggtaggta acttcgattt cgtgatggaa
     6121 tctgcctacc ggcaaagtta tttgccattt atcgaaatcg tcgaagaatt cagctcgatc
     6181 cacatcacct ttcacttcag cggttgtctt ttggaatgga tcgaggcgca tcatccggat
     6241 tacctcgatc gcattgccgc cctggttaaa cgcggaaacg tcgagattat ctccggtgga
     6301 tttttcgaac cggtgctggc tatgattccc gacgccgata aagtcggtca aattaaaatg
     6361 atgaatgatt atattcaaca gcgttttaat tatacggcta aaggactgtg gctaacggaa
     6421 cgagtctggg aacctcatct cgccaaacca attgccgaag ctggaataaa atacatcacc
     6481 gttgacgatt atcatttttt aagcaatggt aaacaggaaa gcgaactgac tggccatttt
     6541 ctgactgagg aacaaggcaa gaccctcggt attttcccaa tcagtcagcg attgcgctat
     6601 accattccat tccaggatcc gcaggcaact attgatcatt tggctaaatt cgccaccgac
     6661 gacggtgaaa atgttattgt tatggcggat gacggcgaaa aattcggcgt ctggcccgga
     6721 actcacgagc aggtttttac caaaggctgg ctgaggaatt ttttcgcggc tttgctgaaa
     6781 aatagcgagt ggctcaagac tttgaccttt gccgaatggt acgaaaatca tccgcccaaa
     6841 ggccgcattt atctaccaac ggcttcctac tttgaaatga gtgagtgggc tctgccctgg
     6901 gaggctggcg aaaaatttgc cgatttagtc cacgaattcg aaagcaagaa gcgtattgag
     6961 gaagtccgac cttttatcaa aggtggcatc tggcgcaact ttctctataa atacgacgaa
     7021 agcaactgga tgcagaagaa aatgttgcac ctttcggaac gctttgccga tattgatccg
     7081 acgagcctaa caaagcctca gcaagttgaa ttggggcaag cccgtctgca tctctggcgt
     7141 agtacctgta actgcgccta ctggcatggt attttcggcg gactttatct gccgcacctg
     7201 cgccatgcaa tttataccga actgctaatc tgcgaacggc tgctagataa aatcacgggt
     7261 gcaaaaggct tccgagcgga ggtgtttgat ttcgataccg acggtgaaaa tgaaattatt
     7321 ataaaatacg gcaaccttca tactgctatt gcaccgcatc gtggcggcat gataacggag
     7381 ttatcgctgg taaacaaagc cttcaatctt ttgaataaca ttcagcgtta tcacgaatcc
     7441 tatcaccgca aagtggcatt agccgacaaa gtagaaaatt ccaatgccag cggcagtatc
     7501 catgatctgg tactttccaa ggaaaaggga ctggataagt acctcaagta tgataaatac
     7561 cctcgaaaaa atcttatgga tcacgtgatt cacccgtctg tttcgctgga gcgcttccgt
     7621 gacggcgatt attacgaaga cagcgatttt atcaccggcc attactctcc ggaactcaat
     7681 gaaaaaaccc ggacgttgac tctctccaaa gacggctggg taaattggca aagattcagg
     7741 atcaaaaaga cgattacttt cggaaaagag gcactgaaag ttacctacat attgaccaac
     7801 atcggcgaac gcgagaatgt tttccgcttc ggaccggagt ttaactttgc tcttctgggc
     7861 ggcaattcgc ccgaccgtta ttacatcgcc gacggtgaaa aaaccgccga accggcgctg
     7921 aactcgatcg ggattttaac cggtattaag tctctgggtg ttgtcaatga atgggacaaa
     7981 ttccaagccg aagtaattgc gccccaggcg gtcgaattct ggcgcttccc gatcgaaaca
     8041 gtctcgctct cggaagccgg attcgagcgc atttatcaaa gttcagtaat aattccgtgg
     8101 ttcgatgtca gattgaaccc ggaagaaact gccactttct cgatcgaatt aacgacgact
     8161 gaattataga agtttaaact atattaaccc gattaaatga aaatgactat gataagtagg
     8221 atttttcata ttcaattatt ccgattatat gtttagataa attcaatgaa aattaatttg
     8281 acaaatataa aattataagc aagatatctc atagggaaag tttagagtac taataaattg
     8341 gatactatga cttttgatcc gctaaaaaat acttgcattt caagccgatt aagaggatat
     8401 taaggcgctt tttggttttt tcgatattta aaatgagaac aagactcatc tgttatacag
     8461 aacccattta taaaagggcg attttagcga aattgcccga acaattaaat ccaacaatta
     8521 atacaaaatg tatccgtagc aaacgcttaa agatcacttt ttaatatgat aaaggcttag
     8581 aatcctatat gaaaaaacac ccaactaaat tagtaaaaat cgatcccatc cctattcttt
     8641 tggaaagagc tccccttccg atccgatatt ttacggctga acgtttctta tcgcacgata
     8701 acgaattagt cgagcaactt agacgggatc tgcaattctt taaaccgcga gaacgtctgc
     8761 tagctactca gcaggaagat ggtctctgga aactgaacaa aaactacaaa atcgaagagc
     8821 aacagaaatc aatgcagttt ctgttacagt tgcagaatat gatgcagtta ttggattatg
     8881 gttgcaccaa agatatgcca gctattcaga gaggcttaat cgctattttg aagtatcaga
     8941 aaccggatgg aaaatttcct ttactccaac atcatcaggg cttgacttta tggttattgg
     9001 ttaggtacgg tttagccggt aatccatttg tcgaaaaggg cttccgttgg ttggcaaagc
     9061 gtcaaagaga agatggcggt tggctctcac ctactatgct agccacaggc gaatcggcga
     9121 aaactgctcg cagtggaata tggactactc tggtaattac tcaggctttc agcgctcatt
     9181 cccgtttacg taacaccgag acttgccagc gcgccgcaaa ttttttgctg aatgtgtatt
     9241 tacagcagaa tcacacgacg cttttccccg agccggatgc ctggaaccat ttatatattg
     9301 attattctga aaacggctta ttccgcggcg gcacattgcg gtttctggaa accctgaccc
     9361 ctttgccaaa ttgccattct catcctaact ttaaaaaggc gttggactgg cttatcggtc
     9421 agcaattgcc ttccggtcta tggccagcta ttgccggacg ttccaaagaa ggcgattatg
     9481 ctgtaacttt tagagttatg gtggttctaa gtgaaataga ccgaattgga taatgacata
     9541 aagtgtttat ttgaaaggta ttaaatatgg ctgaaacaat aaaaaataaa gatgaaaaaa
     9601 atgagacggg gcatggtgta ccggaaatat tgccgataat ggctctgcgt aataccgtgc
     9661 tttttcctca acaagtcatc cctatttata ttgggcgcga acgttcattg cgcttaatag
     9721 aggatttacc cgaaactaaa aaacggatcg ttgtggttgc tcaaaaagaa ggtactttag
     9781 aaaatcccga ggtagatgat ttgtatgaat ggggaacgat tgcccaggta ttaaaaatat
     9841 tcaatatgcc ggatggcagt aaaagtgcaa ttgttcaggg gctagaacgc gccaaaattc
     9901 aatcatttat tcaggttgag ccttatttta agggagccat cctcaaagca caggaaattt
     9961 atcagccagg catcgaaata gacgcgatgc tggctaacct ccgtcaacta tatcaaaaat
    10021 tgattaaaat tgccccttac ttaagtgaag aacaggcttc ggtattttcc tcagttcagc
    10081 atcccggccg tctaattgac cgaataattc acttgctcaa tgtgccggtt agtgaaaaac
    10141 aggaaattct ggaagaattg gacgtccgta aacgtctgga aaaagctacc gtaattctta
    10201 atcgagaaat ccaacgcatc cagctagggg agcaaattca gtctgaggtt caggatgaaa
    10261 tttccaaaac tcagcgcgaa tattttctgc gggaacagct gaaagccatt cgcaaggagc
    10321 tgggcgaaga cgaaggcacc ttagagctaa aagaattaga agaaaaaatt aacctatcaa
    10381 aaatgagcga tgaagcccgt aaagttgctg agaaagagct cgaccggctt agaaaaattc
    10441 caccagcctc accagaatac acggtttccc gcacttactt ggactggttg gtagatttgc
    10501 cctggggcat ttatacaacg gataatgtag atattaaaag tgcgcagaaa atactcgacg
    10561 aagatcacta tggtctggaa aaaatcaaag accgaattat cgaatacctg gcagtccgca
    10621 aattgaagca agaacaggat cccaatgcca gtatcaaagg cccaatcctt tgttttgttg
    10681 ggcctcctgg tgtaggcaaa acctcgatgg ggaagtcaat tgcccgcgct atgggtcgca
    10741 aattcgttcg catttcgctc ggaggagtac gggatgaagc tgaaattcgc ggccatcggc
    10801 gcacctatat cggcgctttg cccggccgta ttttgcaaag catccgtaaa gcggaatcga
    10861 gcaatccgat ttttatgctg gatgagattg acaaaatcgg tgcggatttc cgcggtgatc
    10921 cgagttctgc tttactggaa gtccttgatc ctgagcaaaa ttcgactttc agtgatcatt
    10981 acctcgaagt tacctatgat ctctctaaaa ctatgtttat tgccactgcc aatatggtgg
    11041 acactatttt accagcctta agggatcgaa tggaaatact ggaatttagc ggctatatcg
    11101 aagaagagaa gttgaacatt gccaagactt ttttaattcc aaaacagatt aaggaacatg
    11161 gcttaactaa tgtcacagtt gagtttactg ataaagcaat acgttttatt attaatagct
    11221 acacccgaga agcaggagtt cgtaatctcg aacgggaaat tgccaacgtt tgccgcaaaa
    11281 tcgcccgtga agtcgctgtt aataaacgca aaaaaaactt tctaattgat gaaaaagccg
    11341 ttgaaaattt tcttggtcca cagcgctatt ttgtggatat agccgaaaga ctctccaagc
    11401 ctggtgttgc tatcggattg gcttggactt cggttggcgg cgatattctt tttgtagaag
    11461 ccaccagtat gcgaggcacc ggcaagttga atctcaccgg tcagcttggt gatgtaatga
    11521 aggaatcagc tgaagccgca ctcagctata tccgctcaaa tgcgacttta ttgggactcg
    11581 atgaggaagc tttctcgaaa atcgatatcc atattcacgt gccggctggc gcaataccga
    11641 aagatggccc atcggccgga gtaacgattt taacggcggt ttattcattg ttaaaaaagc
    11701 ggaaggtcaa agacaatctg gctatgaccg gtgaaattac tttacgcggg gcggttctgc
    11761 caattggtgg cgtgaaagag aaagttcttg ccgctcaccg ggcgggattg cgccgggtaa
    11821 ttttaccagc ccagaatcgc aaggacttaa tcgaaatccc aaagcagata aaaaatgaga
    11881 tgcgatttga ttttgtaaag gaaatgtcag aagttctcaa ccttgccatt agaaaataaa
    11941 tgtcggttta aattttattg cgtaaatttg cccgctctta ggtaaaacgg acgcttagct
    12001 cagctggtca gagcatcgcc cttacaaggc gggggtcgta ggttcgaatc ccacagcgtc
    12061 cac
//
LOCUS       DAOMJU010000062        11917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5438_length_11917_cov_17.036756, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000062 DAOMJU010000000
VERSION     DAOMJU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11917
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5438_length_11917_cov_17.036756"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLO58_06765"
     CDS             <1..189
                     /locus_tag="PLO58_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQO74526.1"
                     /translation="DESAILTARRLATEEGILVGISAGANLFAALQIARRNRHDCLII
                     VVFPDTGERYLSTELFEE"
     gene            191..778
                     /gene="cysE"
                     /locus_tag="PLO58_06770"
     CDS             191..778
                     /gene="cysE"
                     /locus_tag="PLO58_06770"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HQO74527.1"
                     /translation="MNFYFIEDLRAIWRNDPAAKNMLEILTCYTTLHAIMLYRIAHFL
                     HTYLHIPILPRFISVLARFWSGVEIHPGAKIGKGFFIDHGTGVVIGETAEIGDNCVLF
                     HGVTLGGTGHHKGKRHPTIGNNVLIGAGATLLGPIFVGDNAKIGATTVIINRDVPPNC
                     TVVGTPGHIVKRNGKCVNEPLPLARYKHNDTKCNP"
     gene            1095..2924
                     /locus_tag="PLO58_06775"
     CDS             1095..2924
                     /locus_tag="PLO58_06775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQO74528.1"
                     /translation="MALMQKMRNHTHVILWALLILFVASLTVGGLVGGADLLDVFSQK
                     SRLKDAAGIVDGEKLDAVRFSELIQNEINNYRDQNQELTESDIEQISDYIWNSYVNET
                     LINKQIDRYKLYATDNEIYEVLVNNPPQFLIQNEAFQTNGQFDYQKYLNALNNPQGNE
                     WLAVEEYVRIYLPFEKMQYLIESLAMVSDAEVIEEIVLNQTKANFEALVIPYTLVARD
                     TFAIDKSEIKKYYDANRKNFYVPETRSLDFVVFETKPTPADTFSALQTIESIRERLAN
                     GEDFATLASEFTEDPSGVANGGDLGWFGKGQMVPEFEQAAFALRKGEVSQPVITSFGV
                     HLIKVEDKRVENGRPEVKARHILIKIKTSPETLENIRAQANLFAFDANEYGFQAAADS
                     HKLVVRNTGPFNKDAKYINYFGPFPAAIRWAYSNIPVGTVSEVLTHEMGITVMCLKEI
                     NKEHYRPLSEVENQIKNILTSEKRIEKLKSISEEIYALVTQDNKFDNAIAKYPQCLLD
                     SYTDHPVNAPLKNVQQSNAITGTLLALQPGQISKPVLVANRNLVIIKLHSRQDVTPEI
                     IVQEKEATRMRILNNKKSAFYTAWINDLKANAKIVDNRSNLYY"
     gene            complement(3004..3873)
                     /locus_tag="PLO58_06780"
     CDS             complement(3004..3873)
                     /locus_tag="PLO58_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74529.1"
                     /translation="MSKKSISIIAAIVILVVLLLVNWKNHQTRLAPTSDNGEWFFLQK
                     SDLRKSPHHTKHARIFGKYVRGYNYYILKGIDKVQAHAPDGGEYFTTLKSRQLESPIG
                     YELKIFGKSLIGPPRRSSYSSGATYGAFIEAMNIMYGKGGHDSLDFEHYEALRMQEIG
                     GGKRREGVKFWGYWNSHGFGNHFALVQYSGIGKAVEPRSARPGDFVNIIWKKDGATSA
                     IFLGWFKDKDEKEKIVYWSSQKETNGFGDQIMPVDNIKHLKFVRVTNPEKLFDFNIDM
                     PVNFKVAGDKVVF"
     gene            complement(3884..4252)
                     /locus_tag="PLO58_06785"
     CDS             complement(3884..4252)
                     /locus_tag="PLO58_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74530.1"
                     /translation="MIWFYFCVAVLLVLSGTIIFFKAAWFKSLLQFVIQKDLLLIPGV
                     FEIAIGLGTLYFRDLTHMTWFVYLAGLLMFLDGILSVLMSRRLREMYEWFINIEDRKM
                     RSYAIFLFLVALGYFLAAIR"
     gene            complement(4256..5455)
                     /locus_tag="PLO58_06790"
     CDS             complement(4256..5455)
                     /locus_tag="PLO58_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQO74531.1"
                     /translation="MTQNVSQPQPLVKSQVALVRCDSYEPARVAAAIRRGIDLLGGIE
                     KFAQSGERIVLKPNLLSAHPPEENVTTHPTVIKAIAEIMLENGVKVFIGDSATRGHLA
                     KILRESGMQTVIDELNLPIADFENGKQVYSPDSVRNKKFTIARGVWESDGIFNVPKLK
                     THHLTRITAAIKNLFGCVPGFLKPALHVALPAVDDFCRMLVDLSIFLKPRLTILDAIE
                     AMEGNGPSSGNAYPLKLLAFSSDLVAIDSVVCRMMAIKPEYVRTNYWGAQAGLGKINL
                     EEIEILGDGLDTFSAPDFKVQRQPDLRFNQAWQYRHLKGLVTNRPVIDAQKCKKCGDC
                     IDQCPQQPKALSWNGKMKTQPPQFDYKLCIRCFCCQEVCPHKAIDSYTPLLRRIVDKV
                     YTNIYGA"
     gene            complement(5460..6176)
                     /gene="rsmI"
                     /locus_tag="PLO58_06795"
     CDS             complement(5460..6176)
                     /gene="rsmI"
                     /locus_tag="PLO58_06795"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQO74532.1"
                     /translation="MKSGILYLVATPIGNLADITFRAIETLREVDLIAAEDTRHAAIL
                     LRHYEIRKPVVSYYEENEKRRSQELLDKLSAGQNIALITDAGTPLLSDPGFQLVRRAI
                     ENDIQIVPIPGASSILTALMVSGLPTDRFTFEGFLPRKKGRMTRLNELANEPRTMVFF
                     ESPHRVLETLQDFLEKFGDRPAVCAREMTKVYEEFRRDRLSNLIKHFENHPAKGEFVI
                     IVEGIKRKSKNHFGSQPQTH"
     gene            complement(6292..7134)
                     /locus_tag="PLO58_06800"
     CDS             complement(6292..7134)
                     /locus_tag="PLO58_06800"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQO74533.1"
                     /translation="MAKKTSVFKIVLFLICVVIILIYGFNRLKDRLPRKLINVIAPTK
                     ITVMTYDINHGMGNDNVYNLSRISKVIKNESPHIVILNEVDYKTRRGLNDEQARRIAA
                     ELGMEFTFSRNYPVDGGWTGNAILSKFPIEFAENKIYKQDYGHNTKSLLHIIVNIGNI
                     KVHVYGTQLSSDSIASSNQVKELLNFVFEWGNDSPVIIGGNFEMTPDYKRIYEMAYYF
                     TDVGAYFQTEAFTYPASEPYKRLDYLFSNKLAIPLKVKVSDNEITRVASDHLPVVAHF
                     KIIY"
     gene            7300..7830
                     /locus_tag="PLO58_06805"
     CDS             7300..7830
                     /locus_tag="PLO58_06805"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HQO74534.1"
                     /translation="MPAKPVKYFIGALFSDKNLLAESLRRAENLYGTADLVSADFPFN
                     LTDYYTSEMGSPIFRRFVSFSKLFSPDFLASAKIHTNSIEDDLRLDGLRKVNLDIGYV
                     DYDKVVLASAKYGIHKIYLGQGIYADLTLHYVKGHYQPYDWAFQDFKLPDYHSFFLKM
                     REIYKQQIKQMLLACQ"
     gene            complement(7850..8734)
                     /locus_tag="PLO58_06810"
     CDS             complement(7850..8734)
                     /locus_tag="PLO58_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQO74535.1"
                     /translation="MNHNRNHNQNTDNNIGLAFGLNLGFAIFELFGGLWVNSIAVISD
                     ALHDFGDSLSLGVAWYFKRLSRRQKDNRFTYGYQRYALLGAIITMIVLVVGSVIILWE
                     AFPRLLHPTIPNSTGMFVFAIVGIVVNGFAVLRLKGYSSLTEKAITWHLMEDVLGWIA
                     VLIVSVILKFTDWYFLDPLISILITIFILYKVIGNFRATMAVFLQSAPANIDNDKIVA
                     HLSQLEKVKSAHHTHIWSLDGEHHVLTTHIVVEADTSKDEVLMIKNRIKELAEKYNFE
                     HLTVEIEYEGEDCSMNCD"
     gene            8954..10204
                     /gene="pepT"
                     /locus_tag="PLO58_06815"
     CDS             8954..10204
                     /gene="pepT"
                     /locus_tag="PLO58_06815"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HQO74536.1"
                     /translation="MDKEILEHFFRYVKIDTQSQEDVTDRYPSTAKQLDLAKILVKEL
                     IDLGLKDAAMDEFGIVMATLPSNLAPTEAARIPVIGLIAHLDTSPEVSGKDVKPIIHQ
                     NYRGGDIKLPGDPNVIIRQSENPILAEKIGEDIVTSDGTTLLGADDKAGIAEIMSFLS
                     YLQKHPDIKHGTLRIAFTPDEEVGAGTDHFDVKKFGADFAYTVDGETVGEIEIETFNA
                     SAGIFTVHGINVHPGYAKGKMVNAIPIAAEIVRLIDREPSPATTEKREGYLHPYVIEG
                     GVEKVTLRVLLRDFDADGIRAKTVRLNEIRDQVASQFPLARVELEVKESYKNMYVKLA
                     ENPKVIEYALEATRRAGIQPKTQIIRGGTDGAKLCFMGLPTPNIFTGGHNFHSKQEWI
                     SVQDMEKTVATLVNLVQVWVEKSL"
     gene            10246..>10432
                     /locus_tag="PLO58_06820"
     CDS             10246..>10432
                     /locus_tag="PLO58_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74537.1"
                     /translation="MFQSIKKSIPLWAIINLICLGIFMTLSNVTCGDKVDNSKADLAY
                     VKAELNKLAPVELKCDLS"
     assembly_gap    10433..10532
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10533..11789
                     /locus_tag="PLO58_06825"
     CDS             <10533..11789
                     /locus_tag="PLO58_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HQO74538.1"
                     /translation="EDMTREEFEKFIAEHPEQKKAFESEFTIIKRQKGQLVAIPYHQE
                     YQPYINAISNLLIEAAEITQDPTLKKYLNQRANDLKTDDYFQSDMDWMDLAGDLEIVI
                     GPYEVYEDNLFNYKAAYEAFICMVDHAESEKLAKVTKYLDEIEANLPLAKEYRNYSRG
                     KVSPIKVVQEIFSSGDTKAGVQTTAFNLPNDERVRSAKGSKKVMLKNVAEAKYEKCWI
                     PIVNEILASEPLKNVSFDAYFTHVLMHEVCHGLGPGILKNPDGSETTVRNELKECYST
                     IEECKADVLGIFSYLYLIDKGVFAQDSKYTALATYLGGMFRSIRFGINEAHGGCVAIQ
                     FNYFIDKGAFLTDAEGKLTIAPAKLETAIKDLAAELLLIEAKGDYEGAKAIIEKYRIA
                     TPLITETVKKLEHLPIDIRPIYPITF"
BASE COUNT         3581 a         2599 c         2308 g         3329 t
ORIGIN      
        1 gatgaatcgg ctattttgac tgcccgccgt ttggcaaccg aagaaggtat tctggtgggt
       61 atttcagcgg gcgccaatct gttcgccgct cttcaaattg cccggcgtaa tcggcacgat
      121 tgtctgatta ttgtggtttt cccagacacg ggcgaacgtt atttgagcac agaattgttt
      181 gaggaataga atgaattttt attttatcga agaccttcgc gccatttggc gcaacgatcc
      241 agccgccaaa aatatgcttg aaattttaac ttgctatact actctacacg caattatgct
      301 ctatcggatt gcgcattttt tgcataccta tttacacatt cccattctgc cacgcttcat
      361 cagtgtatta gctcgcttct ggagcggagt tgaaattcat cccggcgcaa aaatcgggaa
      421 aggattcttc attgatcatg gtacaggagt tgtcatcggc gaaaccgccg aaatcggtga
      481 taattgcgtt ttgtttcatg gtgttactct cggcggcact ggtcatcaca aaggcaaacg
      541 ccatcctaca atcggtaata acgtccttat cggcgccggt gcaactcttc tcgggccgat
      601 ttttgtggga gacaatgcca aaattggcgc cacgaccgtt ataattaacc gtgatgttcc
      661 acctaactgc acagttgtcg gtactcctgg tcatattgtc aagcgcaacg gcaagtgcgt
      721 taatgaacca cttccgctgg cacgttataa acacaatgat acgaagtgca atccataaaa
      781 tatgaagaaa agcggaggga ttttctctct gaaaccgaca attttaattt cggtgtcccc
      841 gaggtgagca tcaggattgt tcatttcata gatacttact caaaatacct aagacatccc
      901 caaaaattag gatacagacg ggataactag ctaaataaca gcataattca tagacttaaa
      961 aagccttatc tattttccta aaaatttgag tgaagtcctg cttatataac tttttgtcaa
     1021 tcccggcaat tttacttata tttagcgtca atttttgaag tggttaatat aaaattcgga
     1081 ggtcttgata aactatggct ctaatgcaga aaatgcggaa tcacacccat gtaattctct
     1141 gggcgctttt aatcctattt gtagccagtt tgacagttgg tgggctagtt ggaggtgccg
     1201 atttactgga tgtcttttcc cagaaatccc gtctcaaaga tgccgctgga attgtcgatg
     1261 gcgagaaatt ggacgcggtg cgcttttcgg aattaattca aaatgaaatc aataattacc
     1321 gtgaccaaaa tcaagaattg acagaatccg atattgaaca gatctccgat tatatctgga
     1381 attcatacgt caacgagacg ttgattaata aacagatcga tcgctacaaa ctctacgcga
     1441 ccgataatga gatttatgaa gttctggtta ataatccacc tcagtttttg atacaaaatg
     1501 aagcgtttca aactaatggc caattcgatt accagaaata tctaaatgct cttaacaacc
     1561 cgcagggcaa tgaatggtta gctgttgagg aatatgttcg tatttacttg cctttcgaaa
     1621 aaatgcagta tttgatcgaa agtttagcga tggtttcaga tgccgaagtc attgaagaaa
     1681 tcgtgttaaa tcagactaaa gccaatttcg aagcgctggt aataccttac actttggtcg
     1741 ctcgggatac ttttgcaatt gataaatcag aaataaaaaa atattatgat gccaaccgca
     1801 agaattttta cgttccagaa actcgctctt tggattttgt agtctttgag accaagccca
     1861 ctccggcgga tactttctcc gctttacaga caatcgaatc tattcgggaa cgcttggcta
     1921 acggcgaaga ttttgccact ttggcttcag aattcaccga agacccgagc ggtgttgcca
     1981 acggtggcga tctgggttgg ttcggcaaag gtcagatggt acccgaattc gagcaagccg
     2041 cttttgcttt acgtaaaggt gaagtatccc agccagttat cacatctttc ggagttcatt
     2101 tgattaaagt cgaagataaa agagtcgaga acggccgacc agaagtcaaa gcccgtcata
     2161 ttttaatcaa aatcaaaact agcccggaaa ccttagagaa tattcgcgcc caggctaatc
     2221 tatttgcctt cgatgccaat gaatacggtt tccaagccgc ggctgattca cataaattag
     2281 ttgttcggaa tactggacca tttaacaagg atgccaaata tataaattac ttcggtcctt
     2341 ttcccgccgc catcagatgg gcttattcca atataccagt cggcactgta tcggaagtcc
     2401 tgactcatga aatgggcatc acggttatgt gccttaaaga aatcaataaa gaacactatc
     2461 gtcctttgtc agaggttgaa aatcaaatta aaaatatcct aacctcggaa aaaagaatcg
     2521 agaaattaaa atctatcagc gaagaaattt atgctttagt aactcaggat aataaatttg
     2581 acaatgccat cgccaaatat cctcagtgcc tgctcgattc ctataccgat catcccgtca
     2641 atgcaccact gaaaaacgtc cagcagtcca atgctattac cggtacgtta ttagctttac
     2701 agcccgggca aatatctaaa ccggtgttgg tcgctaatcg taatctggta atcatcaaac
     2761 tccattcccg tcaggacgtt actccggaaa ttatcgtcca ggaaaaagaa gccacccgga
     2821 tgcgaatttt aaacaataaa aagtcggcat tttacacggc atggatcaat gatttaaaag
     2881 ccaacgccaa aatcgttgat aatcgcagta atctgtatta ttaaaaattc aattatatca
     2941 taaaagtaaa agcccggcga tctaccgggc ttttttattc aaaatattaa aacaatttct
     3001 ccatcaaaat acgactttat cacccgccac tttgaaattg accggcatat caatattaaa
     3061 atcaaataat ttttccggat tggtcactcg tacgaattta aggtgcttga tattgtcaac
     3121 cggcattatt tgatcgccga agccgttggt ttccttttga cttgaccaat agacgatctt
     3181 ttccttctcg tctttatcct tgaaccatcc gagaaaaatg gctgatgttg caccgtcttt
     3241 tttccaaata atattgacga aatcgcctgg tctggcactc cgaggttcaa ctgcttttcc
     3301 aataccgcta tattgaacca gcgcaaaatg attaccgaaa ccgtgcgagt tccaataacc
     3361 ccaaaatttg acgccttctc ttcttttgcc gccgcctatt tcttgcatac ggagagcctc
     3421 ataatgttca aaatcaaggc tatcatgtcc acctttccca tacataatat tcatcgcctc
     3481 gataaaagct ccgtaagtag cgccgctact ataactgctc cgacgaggcg gcccaatcag
     3541 cgacttaccg aaaattttta gctcatagcc gattggtgat tcaagctgtc gagattttaa
     3601 agtagtaaaa tattctcccc catcaggtgc atgcgcctgg actttatcaa tacctttcag
     3661 aatatagtaa ttgtacccgc gcacatattt tccgaaaatc cgggcatgtt tagtatgatg
     3721 cgggctttta cgcaaatccg atttttgtaa aaaaaaccat tcaccattat cagaggtcgg
     3781 agcgagtcgg gtctgatgat ttttccaatt gacgagtagc agaactacca gaattacaat
     3841 cgccgcaatg atggaaattg atttcttact caaattttac tcatcatcta atcgccgcta
     3901 aaaaatagcc aagcgccact aaaaatagga aaatggcata cgaacgcatt ttgcgatctt
     3961 caatgttgat aaaccattca tacatttcac gcaaacgtct tgacattaac accgatagaa
     4021 taccatccag aaacataagc aagccagcta gatatacaaa ccaggtcata tgagtcaagt
     4081 ccctgaaata aagcgttccg agaccgatag caatctcaaa aacacccgga atcaataata
     4141 ggtctttctg gattacgaat tgaagcaggg atttgaacca ggcggcttta aagaaaataa
     4201 tagtcccaga tagaaccagc agaactgcca cacaaaaata aaaccagatc attattcaag
     4261 ctccataaat atttgtatat actttatcca caattcgtcg taaaagcggt gtataactgt
     4321 caatggcttt atgcggacaa acttcctgac agcaaaaaca gcgaatgcac aatttataat
     4381 caaattgggg cggttgcgtt ttcatttttc cattccagga caaggcttta ggttgttgcg
     4441 gacattgatc aatacaatca ccacactttt tacatttttg ggcatctatc actggtcggt
     4501 tggtcactaa ccccttcaag tggcgatatt gccaggcttg attaaagcgc aaatccggtt
     4561 gacgttgaac cttaaaatcc ggagctgaga aagtatccag accatcaccg agaatttcaa
     4621 tttcttccag attgatcttg cccaacccgg cttgagcacc ccaataattg gttcggacgt
     4681 attcaggttt aattgccatc atccggcaca ctaccgaatc aattgccacc aaatcactcg
     4741 aaaacgccag cagtttaagt ggatacgcat ttccgctact cggtccattg ccttccattg
     4801 cctcaatagc atccaaaatc gtaagacgcg gtttcagaaa aatgctcaaa tcaaccagca
     4861 ttcggcagaa atcatccacc gctggtaacg ccacatgcag agccggtttg agaaaacccg
     4921 gaacacagcc gaagaggttt ttgatagccg ccgtaatacg agtcaaatga tgagtcttta
     4981 gtttgggaac attaaatatt ccatcggatt cccacactcc gcgagcaata gtaaattttt
     5041 tattacgcac gctgtcaggc gaataaacct gcttgccatt ttcaaaatcg gcaattggca
     5101 aatttaattc gtcaataacg gtttgcatcc cgctttcccg taaaattttc gccaagtgtc
     5161 cccgcgtcgc cgaatcaccg ataaaaacct ttactccgtt ttctaacata atctcggcta
     5221 tcgctttaat aacggtggga tgagtcgtga cattttcctc cggaggatga gcactgagca
     5281 gattcggttt gagcacaatt ctttcgccgg actgggcaaa tttttcgata ccacccagca
     5341 aatctatccc acgacgaata gccgccgcca ccctcgctgg ttcataactg tcacatctta
     5401 ccagagccac ttgagacttc actaacggtt gaggttgtga aacattctgc gtcataattt
     5461 caatgggttt gtggttgcga accgaagtgg tttttagatt tacgtttgat gccctcaaca
     5521 ataattacaa attcgccttt cgccggatga ttttcgaaat gttttattag attgctcagg
     5581 cggtcgcgac gaaattcctc gtaaactttg gtcatctcac gagcacagac cgccggtcgg
     5641 tcaccgaatt tttccagaaa atcctgaagc gtttccagaa cacgatgggg actctcaaaa
     5701 aataccattg tgcgtggttc atttgccaac tcgtttaagc gcgtcatccg ccctttttta
     5761 cgtggtaaaa agccctcaaa tgtaaaccga tcggtgggta agccggacac catcaaagcc
     5821 gttaatattg acgatgctcc cgggatcgga acaatctgga tgtcgttttc tattgctctt
     5881 cttaccagtt gaaaacccgg atcgctcaat agaggcgttc cggcatcagt gatcaacgca
     5941 atattttgac cggcgcttaa tttatcgagt aattcctgac tgcggcgttt ttcattttct
     6001 tcataatagc tgactaccgg tttccgaatc tcataatgac gcaataagat agcggcgtgg
     6061 cgggtatctt cagccgcaat taaatcaact tcccgcagag tctcgatcgc tcgaaaggtg
     6121 atatcagcca aattgccaat cggtgtagcc accagatata aaatgccgct tttcatattt
     6181 ccgttatgtt attcagattc tcttaatatt ttaattaatt ctacaatttt ttaggttata
     6241 aattcgcccg attatgccat ttcgaataaa aggaaaatgc cttgttaatt attagtaaat
     6301 gattttaaaa tgcgccacca ccggcaaatg atcggaagcc acccgcgtaa tttcattgtc
     6361 cgacactttt acctttaacg gtatcgccag tttattcgaa aaaagataat ccaggcgctt
     6421 gtaaggttcc gaagccggat aggtaaatgc ctccgtttgg aaatatgccc cgacatccgt
     6481 gaagtaataa gccatttcgt aaatacgctt atagtccggc gtcatttcaa aattaccacc
     6541 aattatgacc ggactatcgt ttccccattc aaaaacaaag ttcagcagtt ctttgacctg
     6601 attcgagctg gcaatcgaat cactacttaa ctgcgttcca tatacgtgaa ccttaatatt
     6661 tccgatatta acaatgatat gaagaagact tttggtatta tggccataat cctgtttgta
     6721 aattttattt tcagcaaatt caatcggaaa ttttgataaa atagcattgc cggtccagcc
     6781 gccatcaacg ggataattgc gagaaaaggt gaactccatt ccgagctcag cggcgattcg
     6841 tcgggcttgt tcatcattta aaccacgtct ggttttgtaa tccacctcat tgaggatgac
     6901 tatatgcggc gattcatttt tgatgacctt ggaaatgcgc gatagattat agacattatc
     6961 atttcccata ccgtggttaa tgtcatacgt cattacagta attttagtcg gcgcgatgac
     7021 attaattaat ttacgtggta aacgatcctt aagccggtta aatccataaa ttaggataat
     7081 tacaacgcag attaagaaaa gaacaatttt aaagacagat gttttttttg ccatataaag
     7141 ttggttaagt ttaaaataat aaaattcaaa aaactgattg gaattttacg aatttttctt
     7201 taattggcaa ttgctttatc gtttttgggg tgttttagga ataactccct gatttgaaaa
     7261 tttcaaaatt gataatagat taactcataa atgaagccaa tgcctgctaa accggtcaag
     7321 tatttcatcg gcgccctttt ttccgataaa aacctcctag ctgaatcgct ccgccgagcc
     7381 gaaaaccttt acggcacagc agatttagtc agcgctgatt ttcccttcaa tcttacggac
     7441 tattacacat cggaaatggg tagtccgatt tttcgtcggt tcgtttcatt tagtaaatta
     7501 tttagtcccg atttcctggc atcggctaaa attcacacca actctatcga agacgatttg
     7561 cgcctcgatg gactccgtaa ggtcaactta gacattggtt atgttgatta cgataaagtc
     7621 gtattggctt cagctaagta tggaattcac aaaatttatc tcgggcaagg tatctatgct
     7681 gacttgactt tacattatgt caaaggtcac tatcagcctt acgactgggc ttttcaggat
     7741 tttaaattgc cggactacca ctcatttttt ctgaaaatgc gcgaaattta caaacagcaa
     7801 attaagcaaa tgcttctagc ttgtcagtaa tagtcaatta tattatcact cagtcacagt
     7861 tcattgaaca atcttcacct tcgtattcta tctcgacagt aagatgctca aaattatatt
     7921 tttcagccag ttccttgatg cgatttttaa tcataagcac ttcgtcttta ctggtatctg
     7981 cctcgactac aatatgcgtc gtcaagacgt gatgttcgcc gtccagcgac cagatatgag
     8041 tatgatgagc agatttgact ttctctaact gtgagaggtg cgcaacaatt ttatcattat
     8101 caatattggc tggcgccgat tgcagaaaaa cagccatagt cgctcgaaaa ttgccaatta
     8161 ctttatacag aataaaaatt gtaatgagaa tcgaaatgag cggatccaga aaataccaat
     8221 cggtaaattt caatatgaca ctcacgatta aaaccgcaat ccagcctaac acatcttcca
     8281 tcaaatgcca ggtaatcgct ttctcagtca acgacgaata gccttttaga cgtagaacag
     8341 caaatccgtt gacaactatt ccaactatgg caaaaacgaa cataccagtc gaattaggta
     8401 tggtgggatg caaaaggcgt ggaaaagcct cccataaaat tatcaccgag ccgaccacca
     8461 gaactatcat agtaataatc gctcctagaa gagcataacg ttgataaccg taagtgaaac
     8521 gattatcctt ctggcggcgc gaaaggcgct tgaagtacca ggcaacgcct agtgacaggc
     8581 tatcgccaaa atcatgcaac gcgtcggaaa ttaccgcgat actattcacc cacagtccac
     8641 caaacaattc aaaaatcgca aatccgagat tcaaaccaaa agccagtccg atgttattgt
     8701 cggtattttg gttatgatta cgattatggt tcatattata aattcctttt taagggaatc
     8761 acaggctcac gattggtggt ttcattacgg atgtaggagt ttttgttttt atataatgtt
     8821 gtgaagtatt gataaaacac atttgtttct catcaacaga atttctttcc aaaagcactt
     8881 tttaatatac aaattgttat caataattac tattttatag ccagtgaaat taggagacag
     8941 aaaggaataa ttcatggata aagagatact cgaacacttt tttcgttacg tgaaaatcga
     9001 cacccaatcc caggaagatg tcactgaccg ctacccgagt acggcaaaac aactcgattt
     9061 agctaaaatt ttagtgaaag agttgattga cctcggatta aaagatgccg ctatggatga
     9121 attcggaatc gttatggcaa ctttgccttc caatttagcg ccgactgaag ccgccaggat
     9181 tcccgttatt gggctgatcg ctcatctcga tacttcaccg gaagtcagcg gtaaggacgt
     9241 taaaccgatt attcatcaaa attatcgagg tggagatatt aaattaccag gtgatcctaa
     9301 cgtcatcatt cgtcaatccg agaaccctat cttagccgaa aaaatcggcg aggacattgt
     9361 cacttccgat ggtacaactc tattgggcgc cgatgataaa gccgggattg ccgaaataat
     9421 gtcttttttg tcttacttgc aaaagcatcc agacatcaaa cacggcactt tgcggatcgc
     9481 tttcactccg gatgaagaag tcggtgccgg tactgaccat ttcgatgtta agaaattcgg
     9541 ggctgacttt gcttataccg ttgacggtga aacagtcgga gaaatcgaaa ttgaaacctt
     9601 caacgcaagc gccggaattt tcacggttca tggtatcaat gttcatcccg gttacgctaa
     9661 aggcaaaatg gtcaatgcca ttccgattgc ggctgaaatt gttagattaa ttgatcgtga
     9721 accgagtccg gcaactactg aaaaacgcga gggctatctc catccttatg tcattgaagg
     9781 cggcgttgaa aaagtcaccc tacgcgtttt attacgcgac ttcgatgccg atggcattcg
     9841 cgccaaaacc gtccgtttga acgaaattcg cgatcaagtc gcctcccaat ttccactggc
     9901 gcgtgttgaa ctggaagtca aggaatccta taaaaatatg tacgtaaaac tagccgagaa
     9961 tcccaaagtc atcgaatatg ctctggaagc tacccgacgt gccggaattc agcccaaaac
    10021 tcagattatc cgaggtggca ctgacggcgc caagctctgt tttatgggtc ttccgactcc
    10081 caatattttt accggcggtc ataattttca tagcaaacag gaatggattt ccgtgcaaga
    10141 tatggaaaag accgttgcaa ctttggtgaa tctcgtccag gtttgggtgg aaaagtcgct
    10201 gtaatttatt ttattgaaca tgaaataata caaaatggag gaaaaatgtt tcaatctatt
    10261 aagaaatcaa tcccattatg ggcaattatc aacctgatct gtctgggaat ttttatgact
    10321 ttaagcaatg taacgtgtgg tgataaagtg gataatagca aagccgatct ggcttatgta
    10381 aaagctgaac ttaataaact tgcgcctgtg gaactgaaat gtgacctatc ctnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaagatat gaccagggag gaattcgaga
    10561 aattcatcgc cgaacatcct gaacagaaaa aggcttttga aagtgaattt accatcatca
    10621 agcgccaaaa aggtcaactg gtcgccattc cttatcatca agaataccaa ccatatatca
    10681 atgcaatttc taatttgttg atagaagccg ccgaaatcac tcaagacccg actttaaaaa
    10741 agtatctcaa ccaacgggca aatgatctta aaaccgatga ttatttccag agcgatatgg
    10801 attggatgga cctggccggc gacctggaaa tcgtcatcgg accgtatgaa gtgtacgagg
    10861 ataatctttt caattacaaa gccgcttacg aagccttcat ttgtatggtt gaccacgccg
    10921 aaagcgaaaa attagccaaa gtaaccaaat atctcgatga aatcgaagcc aacctgccac
    10981 tcgccaaaga atatcgcaat tacagtcgcg gtaaagtatc gcctattaaa gtcgtacagg
    11041 aaattttttc atctggtgac accaaagctg gagttcaaac aacggctttt aatttgccaa
    11101 acgacgagcg ggtgcgttcc gccaaaggct ccaagaaagt aatgttgaaa aatgtagccg
    11161 aagccaaata cgaaaaatgc tggattccga ttgtcaatga aattttagcg tccgaaccac
    11221 ttaaaaacgt ctcattcgac gcctatttta cgcacgtgct tatgcacgaa gtctgccatg
    11281 gtctcggacc gggaatcctg aaaaatcccg acggtagtga aactaccgtg cgcaatgaat
    11341 taaaggaatg ttactccact attgaggaat gcaaagccga tgtgctgggc attttttcct
    11401 acttgtactt aattgataaa ggcgttttcg ctcaagactc aaaatacacg gctctggcaa
    11461 cttatctcgg cggaatgttt cgttcaatac ggttcggcat caatgaagcc catggcggat
    11521 gtgtagctat ccaattcaat tactttattg ataaaggtgc attcttgacc gatgcagaag
    11581 gcaagctgac tatcgcccct gctaaactcg aaaccgcaat taaagacctt gcggcggaat
    11641 tacttttaat cgaagccaaa ggcgattacg aaggcgccaa agccattatc gaaaaatatc
    11701 gtattgcaac tccgctaatc accgaaactg tcaagaaatt ggagcatcta ccgatcgaca
    11761 ttcgtccgat ttatccgata acgttctaac tatttaaatt aaataagcca ctcaacggaa
    11821 ggtcaaaatt caatcttaga ttcgatttaa actcaatcga tagttagata ctttaagaaa
    11881 agatcaatat aaaaaattag tccaaaaaat aactaat
//
LOCUS       DAOMJU010000063        11878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5460_length_11878_cov_19.706166, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000063 DAOMJU010000000
VERSION     DAOMJU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5460_length_11878_cov_19.706166"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..734)
                     /locus_tag="PLO58_06830"
     CDS             complement(3..734)
                     /locus_tag="PLO58_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQO74539.1"
                     /translation="MRFAIIADIHGNLAALEAVLADIDQQQIDRIVDLGDFVGYGPEP
                     EDVTQLLRELQIAGIVGNHEVALTDDELLNYFSVDAFESIIITRQLISEQTLEYIKTL
                     PVFLTVGNLRFVHGTPPDSYKDYINYYGEHELRKAFASTPEWITFVGHTHQLGLYESD
                     AENKNIHTSILKKGCRQLSRQSKYIINIGSVGQPRDENNSAKYVIFDEETDALEVRAI
                     PYDVEHTIRLIQDIGLPESNAYRLL"
     gene            complement(1273..2166)
                     /locus_tag="PLO58_06835"
     CDS             complement(1273..2166)
                     /locus_tag="PLO58_06835"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HQO74540.1"
                     /translation="MFLILINQNIGEEKMIYHKLTPDEEKVIIHKDTERPFSGKYYDF
                     YEDGIYTCKRCDAPLYRSRDKFDAGCGWPSFDDEITGAVKRVLDQDGRRTEIVCANCG
                     AHLGHFFTGENFTAKNNRHCVNSISLNFIPAKDTSETALAIYAAGCFWGVEYYFKQAP
                     GVLATRVGYTGGHTTNPTYKQVCSGKTGHKEAIEVTYDPTVTSYEELTKLYFEIHDFT
                     QTDGQGPDRGEQYLSYIFYLDEFQKKVAEDLIALLKRKGFAVATKIEKAGAFYPAEDY
                     HQDYYTKTGKKPYCHFRKKIF"
     assembly_gap    2173..2272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2392..2886
                     /locus_tag="PLO58_06840"
     CDS             2392..2886
                     /locus_tag="PLO58_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQO74541.1"
                     /translation="MKYHYCYHCGTPTTSLEMEGYRRAFCPQCQLILYENPLPTVVII
                     AYNEQNEIVLIRRAVEPGRGGWSLPGGFIEIGETAAQAAVRELCEETGLSATELKLVD
                     VGTHLNGFYGDILMIGYATPVQEDLIRPGSDAAEAAWFKIDDHPSLVFPFHEKLIENW
                     RMIK"
     gene            2868..3956
                     /gene="mnmA"
                     /locus_tag="PLO58_06845"
     CDS             2868..3956
                     /gene="mnmA"
                     /locus_tag="PLO58_06845"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQO74542.1"
                     /translation="MANDKITLVTKKQKVLVALSGGVDSAVAAWVLCQEGYEPVGITF
                     RMWRMKEDIAQAKAIDRARQVCEILKIPHIIFDLNAEFEEQVVKPFVNEYFQGLTPNP
                     CVNCNRVIKWHNLQKMADQYGIEKIATGHYARLKYNSANKRYQILKGVDSTKDQSYAL
                     WQIPQTALKRTLFPLGEMTKNAVKTIAVDHNLIVGKITESQNICFIPEGDYRSFLKEY
                     APQKVQTIGRGELVDERGRVLGWHNGFYNFTIGQRKGFRVGFKERRYVKAINAPKNRV
                     VIAPDKSLFSSKMIIKNVNWVGISAVAEFDGIIKIRYNHPGAMGHGKRINDNEYEITF
                     IEPQRAICPGQSAVVYQDDCLLMGGIIQ"
     gene            4023..4823
                     /gene="panB"
                     /locus_tag="PLO58_06850"
     CDS             4023..4823
                     /gene="panB"
                     /locus_tag="PLO58_06850"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQO74543.1"
                     /translation="MQDKVRVSTILEMKQKGVKITSLTAYDFTMAHLMDESGVDIILV
                     GDSCGNVCAGYETTLPVTMDEMVYHVQCVRRGVKRALLVADMPFMSYQESVEQALHNA
                     GRFMKECGAEAVKLEGGEPICPVVEKMVEIGIPVMGHLGFTPQSVHKFGGFGVRGKET
                     EEAKYIEKSAFALQDAGAFAIVLEKIPAKLAADVTAQLRIPTIGIGAGKECDGQVLVS
                     YDMLGLFDQVKYKFVRRYANLADEIRRATTAFGNDVRNGNFPDESESY"
     gene            4847..>5103
                     /locus_tag="PLO58_06855"
     CDS             4847..>5103
                     /locus_tag="PLO58_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020059576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQO74544.1"
                     /translation="MQILTKIEDIRQELLVIRTSAKKIALVPTMGYLHEGHLSLVDVA
                     KVNADVVVMSIFVNPTQFAPNEDLMRYPRDIERDERLARERG"
     assembly_gap    5104..5203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5204..5464
                     /locus_tag="PLO58_06860"
     CDS             <5204..5464
                     /locus_tag="PLO58_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQO74545.1"
                     /translation="QQAPIIFKALQAARENVKNGLTDANQVRDQITKMIQTSPLARID
                     YVEIIDDQTLTPVETVKPGTFAAVAVYYGKTRLIDNIYLKER"
     gene            5550..6029
                     /locus_tag="PLO58_06865"
     CDS             5550..6029
                     /locus_tag="PLO58_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74546.1"
                     /translation="MKRNFLTVTMLAIFLGTSGFAQLKTQIQRSPTIGEAIHLPGISS
                     AISNYSLFDPSRFKMQQSYSLSYSTFGNQSMSVGVYQNAMSFLLSNKFLLNTRFGFYH
                     NPLQLGTFNATSNNMLDNLIFGADLIYRPKENATFMIRFDRRPYYYPYEYYNPYLGY"
     gene            6032..6571
                     /locus_tag="PLO58_06870"
     CDS             6032..6571
                     /locus_tag="PLO58_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HQO74547.1"
                     /translation="MARSRNRRKRPTSRKNKRRAVDLQKWFLNLAIFSISVVIVGFIF
                     SMGKRFNNPNEKLTMSQVNVIPPDERPVPYIVIEVLNGTDVPGLAQKFTNFLRQNGYD
                     VISTGNADYSGYEKTMLITRTADVTKLREVNGTLLLDSDQLFQKIDSTLQVDLTLIVG
                     KDYQRLPVYSKIIAIGETF"
     gene            6575..6958
                     /gene="rsfS"
                     /locus_tag="PLO58_06875"
     CDS             6575..6958
                     /gene="rsfS"
                     /locus_tag="PLO58_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQO74548.1"
                     /translation="MKTSQNTTDKNQSYPLARQIADIAVDKKARDVVIIDLRPLTSIT
                     DYFVICTGAVDQHVKAIVDEIDDRLRPAEKPWHIEGYQHLSWVLMDYVNVVVHVFNPE
                     ARAYYNLERLWADAATEIISDDSAD"
     gene            7009..7920
                     /gene="queG"
                     /locus_tag="PLO58_06880"
     CDS             7009..7920
                     /gene="queG"
                     /locus_tag="PLO58_06880"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQO74549.1"
                     /translation="MSLTAEIIRLAENSGLARVGISPPFLDYKYITFFQQWIDAGFLG
                     TMSWMRKYYDFQNYQKTYFSWAKSVLVVADNYFQNVEWPTGIPKIARYAWGKDYHKVV
                     KQKLAKVSREINLLEPATVSRIYVDSGPVMQKIFAWQAGLGWVGKNNLLIVPDLGSYC
                     FIGMIFLNLELHFNEPQSNLCGDCQLCRDACPNQALVDEHLLDARLCTAYLTIEKDDE
                     FSAAEKKNLRGWIYGCDTCQKICPYNQKWKRPGDARYSEYYSEFSKSLTDWLKVTEDE
                     FGEIFRDSPIRRIGFANWRRNLLTLRK"
     gene            8000..>8590
                     /gene="argS"
                     /locus_tag="PLO58_06885"
     CDS             8000..>8590
                     /gene="argS"
                     /locus_tag="PLO58_06885"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQO74550.1"
                     /translation="MIEHIRGLLEKALQQNDLDYREPIQIERPKQETFGHYTTNLALA
                     VAKIQRQSPLEIAEKLVATLPQSEYIERVEYRAPGFINFFLSPRVLQRIVAEILKAGE
                     NYGKLDIGKNQRVQVEFVSANPTGPLTVGHGRQAVLGDTVARILEWVGYRVDREYYYN
                     DAGRQMRMLGHSVYWRYRQLLGWKGEFPEDHYQGEYI"
     assembly_gap    8591..8690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8691..9485
                     /locus_tag="PLO58_06890"
     CDS             <8691..9485
                     /locus_tag="PLO58_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HQO74551.1"
                     /translation="WFRAGLYGGTDDRVLWKSVVDEATYRLPDIAYHVTKFERGYDLI
                     IDIFGADHHATYPDVLAGLRALGYDTSRVKVLIHQFVTLIKGGEKVKMSTRKANYVTL
                     DELIDDVGRDVARYFFLMRGMKSHLNFDLDLAKTESEENPVFYLQYAHARICSILRNA
                     ATKNLAVGNDFDMTLLKEEETLSLLNALSEFKEVIEICVSTLEPQHLTTYLQKVATAF
                     HKFYTVHRVISKDWELSQARLALVKAVQIVMANGLALLGISAPERM"
     gene            9596..9895
                     /gene="gatC"
                     /locus_tag="PLO58_06895"
     CDS             9596..9895
                     /gene="gatC"
                     /locus_tag="PLO58_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQO74552.1"
                     /translation="MRVDLKTAEKIADLARLTLSEAELERYAAQMDQILEYIEQLQNL
                     DTSNVKPLSHVQEITNVFRNDDVKTSLPKEAVFANAPQEKAGYFVVPKVIKDINP"
     gene            9892..>10163
                     /locus_tag="PLO58_06900"
     CDS             9892..>10163
                     /locus_tag="PLO58_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQO74553.1"
                     /translation="MKVLGVIPARFAATRLPGKPLVSLHGKTLIQRVYENARNSQLIN
                     QLVVATDDQRIFEHVKSFGGECRMTPVELASGTDRCALVARDNEAD"
     assembly_gap    10164..10263
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10264..11811
                     /locus_tag="PLO58_06905"
     CDS             <10264..11811
                     /locus_tag="PLO58_06905"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQO74554.1"
                     /translation="SRGFKVTIQKFDPYINIDPGTMSPFQHGEVFVLEDGSETDLDLG
                     HYERFIDENMNTRNSNTSGKIYYEVINRERRGDYLGATVQVIPHITDEIKRRITAVNV
                     DSAYDVVICEIGGTVGDIESQPFLEAIRQFRFESGRGNCINIHLTLVPYIETSGEIKT
                     KPTQHSVMRLREIGIQPDILLCRSKFHLDESIHKKIALFCNVMPEVVIDTIDAETIYE
                     IPLIFDQQKLGDQITDLLDLPRRKPDLKDLEKFVQAIKNPKDEVNIAICGKYTQLHDA
                     YKSIIESFNHAGAENNTRVNIVWIESEDLTNDCETVNKLKNVDGILVPGGFGERGVKG
                     KINAIKLARENGIPFLGICLGLQCAVVEFARNVCGWKDADSTEFNENTQHPVIDLMEE
                     QKRVFNKGGTMRLGAYTAEVIPGTLAYEIYKNKQISERHRHRYEVNNTYLAEMVKAGL
                     VVGAVNKELNLVEMIELKNHPWFVACQFHPEMKSRINKAHPIFREFVRAARKYHNQKA
                     ENARSQS"
BASE COUNT         3291 a         2289 c         2629 g         3269 t
ORIGIN      
        1 gtttataata aacgataagc atttgattcc ggtagtccaa tatcctggat caaccgaata
       61 gtatgctcaa catcgtaagg aatggctcta acttccaagg catctgtttc ttcgtcgaaa
      121 attacatact tggcagagtt attttcatca cgcggctgac caacactgcc gatattgatg
      181 atatatttcg actggcgact gagttgacga caaccttttt tcaaaatgga agtatgaata
      241 tttttattct cagcatctga ttcatataat ccgagttgat gggtatggcc gacgaaagta
      301 atccattcag gtgttgaggc aaaagctttg cgtaattcgt gttctccgta atagttaata
      361 taatctttat atgagtccgg gggagtgccg tgtacgaaac gcagattgcc tactgttaaa
      421 aataccggca gggtttttat atattccaag gtctgctctg agatcaactg acgggttatt
      481 ataattgatt cgaatgcatc aacggaaaag taattgagca gttcatcatc cgtgagagca
      541 acttcatgat taccaacaat ccccgcgatt tgtagttcgc gtaaaagctg ggtaacatct
      601 tccggttccg gaccataacc gacgaaatcg cccaggtcaa cgatgcggtc aatttgttgc
      661 tgatcaatat cagccaggac ggcttctaga gccgccaggt tgccatgaat atcggctatg
      721 attgcaaatc gcattgctca aactttaaaa ttgaaaaatt attattagga aatgaacctc
      781 ttctgataat cgcttcgctg gtaaaaacta cattcatatt aaaccaagcg caaattacat
      841 aacaataata ttcttttttt gtcaaaaagg caagaacata atcatcacct acctcaaata
      901 tttttaaatt ttaatcaatc caaggtaaat gatttttgaa tcaataattt atacaatact
      961 ttaactatgg tctattttga attctatggt cgttcgattg acatccgccg cgccacagtc
     1021 aaggtattgc agagattcat ctaatccaaa aacatattac ctgatgggga cgaagcaatc
     1081 tatttttctc aattgctttc ggcagtattt cgcttgggtc ttcatcaatc tcaacttacc
     1141 tggtggccgt ttagggtaaa atttagtgag tgcttagatt caatcagtta aaaactatat
     1201 ctgttcttct taacctgaac cagtggattg attataggac gataggtcaa tgtctataga
     1261 cttcttaacc gcttaaaaaa ttttcttccg gaagtgacag tacggctttt ttccggtttt
     1321 ggtgtaataa tcctggtgat aatcttcggc tggataaaat gctccggctt tttctatttt
     1381 tgtagcaacg gcgaatccct tccgttttaa taacgcaatc agatcttccg cgactttctt
     1441 ttgaaactca tcgaggtaaa aaatatagga aagatactgt tcgccccgat ccggtccctg
     1501 tccgtcggtt tgagtaaagt cgtgaatttc aaaatagagt ttggtcaatt cttcataact
     1561 tgtaactgtc ggatcgtagg tgacctcaat agcttcttta tgaccggttt tgccggaaca
     1621 aacttgttta taagtcggat tggtggtatg tccgccggta tagccgacgc gggttgccag
     1681 tacgcccggc gcctgcttga aataatattc tacaccccag aagcatcccg cggcgtaaat
     1741 tgccaatgcg gtttccgagg tatcttttgc cggaataaaa ttcagggaaa tcgaattgac
     1801 gcaatggcgg ttgttcttag ctgtaaaatt ttcaccggtg aaaaaatgtc cgagatgagc
     1861 accgcaattg gcacagacta tttcagtgcg ccgaccgtct tggtcgagaa cccgcttgac
     1921 ggcgccggta atttcatcgt caaaacttgg ccagccgcaa ccggcatcga atttatctct
     1981 cgaacggtag agcggcgcat cacagcgttt acaggtgtat atgccatctt cataaaagtc
     2041 ataatattta ccggaaaatg gtcgctcggt gtctttatga atgatgactt tttcttcatc
     2101 aggcgtgagt ttatggtata tcattttttc ttctccaata ttttggttta tcaagattag
     2161 gaaaatgctg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttacctac
     2281 gaatcgcatt ttccccgaag tacaatctat aacctcaatt agttcaaaga gtttattagc
     2341 aagtaataaa attggttgtt attcgcccga aaactgtcta tttttgaact tgtgaagtat
     2401 cattattgtt atcattgcgg tacgccaaca acttcgctcg aaatggaagg ctaccgacgc
     2461 gcattttgcc ctcaatgtca gctcattcta tatgaaaatc ctctacccac tgtcgttata
     2521 attgcttata acgaacaaaa tgaaattgtt ttaattcgtc gtgcagtcga accaggacgg
     2581 ggcggctgga gcctcccagg tggattcatc gaaataggtg aaaccgcggc gcaagccgct
     2641 gttcgtgaac tttgcgaaga aaccggttta agcgctacgg agttaaaatt ggttgatgtt
     2701 ggcactcact tgaatggatt ctatggtgat attctgatga ttggttatgc tacaccggtc
     2761 caagaagatt taattcgtcc tggcagtgat gcggcggaag ccgcctggtt taaaatagac
     2821 gatcatccat ctctggtttt tccctttcat gaaaaattga ttgaaaattg gcgaatgata
     2881 aaataacctt ggttacgaaa aagcaaaaag tattagtagc tctgagtggc ggcgttgaca
     2941 gcgcagtggc ggcgtgggtg ttatgtcaag aaggctatga accggtagga atcacttttc
     3001 ggatgtggcg aatgaaagaa gatattgccc aggcaaaagc cattgatcgc gctcggcaag
     3061 tttgtgaaat attgaaaatc ccgcatataa ttttcgattt aaatgcagaa tttgaagagc
     3121 aagtagttaa accattcgtc aatgaatatt ttcaaggatt gactccaaat ccatgcgtga
     3181 attgcaatcg cgttattaag tggcataatc tgcagaaaat ggccgaccag tatggaatcg
     3241 aaaaaatcgc caccggtcat tatgcccgtc ttaaatataa ttccgcaaac aaacgctacc
     3301 agattttgaa aggagtggat tcaaccaagg atcaatcgta tgctctctgg caaattccgc
     3361 aaaccgcact gaaacgcacc ctttttccgc tgggcgaaat gactaaaaat gcagtgaaaa
     3421 caattgctgt tgatcataat cttatcgtcg gtaaaatcac cgaaagccag aatatttgtt
     3481 ttatccccga aggcgactat cgtagtttct tgaaggaata cgcgcctcag aaagtgcaaa
     3541 ccattggacg gggcgaattg gtggacgaac gcggccgagt tttaggctgg cataatggtt
     3601 tttacaattt tacaattggt cagcgcaaag gcttccgcgt cggtttcaaa gagcggcgtt
     3661 atgtaaaagc cattaatgcc ccgaaaaatc gggttgtgat tgcgcctgat aaatcgcttt
     3721 tctcttcgaa aatgataata aaaaatgtca actgggtcgg aatatccgcc gtagctgaat
     3781 tcgacggcat aattaaaata cgttataatc atccgggcgc tatgggtcat ggaaaaagga
     3841 ttaatgataa cgaatatgaa attactttta ttgaaccgca aagagcgatt tgtcccggcc
     3901 aatcggctgt agtttatcag gatgattgcc ttctgatggg cggaatcatc caataaattc
     3961 gatgatcaat gtccgacata ttgggaaaaa tatttaaatt ttaagcaaga aatggtgagg
     4021 aaatgcaaga taaagtacga gtctcaacta ttctcgaaat gaaacaaaaa ggcgtgaaaa
     4081 tcacatcctt aacggcttat gattttacca tggcgcactt aatggacgaa tcgggtgttg
     4141 atattatcct ggtcggcgat tcctgcggga atgtctgcgc cggatacgaa actactttac
     4201 cggtgacaat ggatgaaatg gtctatcacg tgcaatgtgt gcggcgcgga gtcaaacggg
     4261 ctttgctggt agccgatatg ccgtttatgt cctatcagga aagtgtcgaa caggcactcc
     4321 ataacgctgg gcgctttatg aaagaatgcg gcgcagaagc agtaaaactc gaaggtggtg
     4381 aaccgatttg tcctgttgtt gaaaaaatgg tcgaaatcgg aattccggta atgggacatc
     4441 ttggttttac tccgcaaagt gtccataaat tcggtggatt tggtgtgcga ggtaaagaaa
     4501 ctgaagaagc caaatatatt gaaaaatcag cgtttgcctt acaggacgcc ggggcatttg
     4561 ccattgtgct ggagaaaatt ccagccaaac tggcggcaga tgttaccgct cagctaagaa
     4621 ttcccacgat tggtattggc gccggaaaag aatgcgatgg gcaggtcctg gtgtcttatg
     4681 atatgctcgg attattcgat caggttaaat ataaattcgt gcgccgatac gctaatctgg
     4741 cggatgaaat tcgccgagcg acgacagcct ttggtaacga tgtacgcaac ggcaattttc
     4801 cggacgaatc tgaaagttat tgaagcagct caaaattaaa taaaatatgc agatattgac
     4861 taaaatcgaa gatattcgtc aggaattatt agtaattcga accagcgcga agaaaatcgc
     4921 gcttgtacca acgatgggct acttacatga ggggcatctc tcattagtag atgtcgctaa
     4981 agtcaatgcc gatgtggtcg ttatgagcat tttcgttaat ccgactcaat ttgccccgaa
     5041 cgaagacctg atgcgttatc cgcgcgatat cgagcgtgat gagcgtttag cccgggaacg
     5101 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaacaag ctccgattat
     5221 tttcaaagct ttgcaagccg cccgagaaaa tgtcaagaat ggtctgaccg acgccaacca
     5281 ggtccgtgac caaattacta aaatgattca gacttcgcca ctggcgcgga ttgattatgt
     5341 cgaaattatt gatgatcaaa cgctgacgcc ggttgaaacg gtaaagccgg gaactttcgc
     5401 cgcggtagcc gtgtattatg gcaagacgcg tttgatagat aatatttatt tgaaagaaag
     5461 ataattttaa agaagtgtga gtttggtctt gacaaactcc cgtaaaagat ttaaaatgta
     5521 gtatgaaaaa tgtcaatggc ggtgttaaga tgaaaagaaa ttttttaaca gtaacgatgt
     5581 tggcgatttt tctcggaacg agcgggtttg ctcagctgaa gactcagatt caaaggtcac
     5641 ctacaatcgg cgaagcaatt catttgcccg gtatttcatc cgcaatatcc aattatagcc
     5701 tatttgatcc cagccggttt aaaatgcaac agtcttattc tttatcctat tcgacctttg
     5761 gtaatcaatc gatgtcggta ggagtttacc aaaatgcgat gtcctttttg ctctcgaaca
     5821 aatttttact aaacacccgc tttggatttt atcataatcc gctgcaatta gggacgttta
     5881 acgctacctc aaataatatg ctcgataatt taatatttgg agctgaccta atatatcgtc
     5941 ctaaggaaaa cgccaccttt atgattcgtt ttgaccgcag accatattac tatccgtatg
     6001 agtattacaa tccttacttg ggatattaag agtggcacgt agtcgtaatc gtcgcaaacg
     6061 ccctacctct cgtaaaaaca aacgccgggc agttgacctc caaaaatggt ttctcaatct
     6121 ggcaattttt tctatttcgg tagttattgt tggttttatc ttctcgatgg gaaaacgctt
     6181 taacaatcca aatgaaaaat taactatgtc gcaggtgaat gtaattccac ccgatgaaag
     6241 accggtaccg tacattgtta ttgaagtctt aaatggaact gatgtacccg gtttagctca
     6301 aaaattcacc aactttctac ggcaaaatgg ctatgatgtt atttcaaccg gtaacgctga
     6361 ttattctggt tacgaaaaaa ctatgctgat aacccgtacg gcggatgtta ccaaattgcg
     6421 ggaagtgaac ggaactttac tgcttgattc cgatcaattg tttcagaaaa tagactcaac
     6481 cctccaggtt gacctgactt tgattgtcgg caaagattac caacgcttgc cagtttattc
     6541 taaaataata gctatcggag agacgtttta atttttgaaa acctctcaga atactacaga
     6601 taaaaatcaa tcctaccctt tagcccggca aattgcggac atcgcagttg ataagaaagc
     6661 gcgtgatgtt gtcataatag atttgcgacc gttgacttcg ataacggatt atttcgtgat
     6721 ctgtaccggc gcggttgatc agcacgtcaa agccatcgtt gatgaaattg atgatcgtct
     6781 gcgaccggct gaaaagccct ggcacatcga aggttaccag cacctcagct gggttttgat
     6841 ggactatgtc aatgtcgtgg tgcacgtttt caatcctgag gcgcgtgcgt attataatct
     6901 tgaaagactc tgggcagatg ccgccacgga aataattagt gatgattctg ccgattgatc
     6961 gtcgagtata atcggcaatg tttttaaatt tttattgaaa aaatattgat gagtctgacg
     7021 gctgaaatta ttcgtctggc tgagaattcc ggattggcgc gtgtgggaat ttctccgcct
     7081 tttttagact ataaatatat cacttttttc cagcaatgga ttgatgcggg atttttaggc
     7141 acgatgagct ggatgcggaa atattacgat tttcagaatt atcagaaaac gtacttcagc
     7201 tgggcaaagt cggttctggt ggtagcggat aattattttc aaaatgtaga gtggccaacc
     7261 gggattccca aaattgcgcg ttatgcttgg ggcaaagatt atcataaagt tgttaaacaa
     7321 aaattggcga aagttagtcg cgaaattaat ttattggagc cggcgactgt atcccggatt
     7381 tacgttgatt cgggtccagt aatgcaaaaa atcttcgcct ggcaagccgg tttaggctgg
     7441 gtcggaaaaa acaatttgct gatcgtcccg gatctcggtt cgtattgttt tatcggaatg
     7501 atttttctga atcttgagct ccattttaac gaaccgcagt cgaatctttg tggcgattgt
     7561 caactttgtc gggatgcttg ccccaatcaa gccctggtag atgagcattt attggatgct
     7621 cggctttgca cggcatatct gactattgag aaagacgacg aattttcggc ggctgaaaag
     7681 aagaatttac gcggctggat ttatggctgt gatacttgcc agaaaatttg tccgtataat
     7741 caaaaatgga agcggccagg agatgctcga tattctgaat attactcgga gttcagcaag
     7801 tcactaactg attggctgaa agttacagaa gatgaattcg gtgaaatctt ccgggatagc
     7861 ccgatccgtc gcattggctt tgctaattgg cgacgcaatc tactgacgct ccgcaagtaa
     7921 attaaattta tacggtaatg acgtttgcac aggaatagac gggcgcttaa atttagaccg
     7981 atttttttaa aggaatgaaa tgatagaaca tatccgcggt ttgctggaaa aagctcttca
     8041 gcaaaacgac cttgattacc gcgaaccgat tcagattgag cgaccaaaac aggaaacttt
     8101 cggacattat accacaaatc tggctttagc ggtggctaaa atccagcgtc aatctccgct
     8161 tgaaattgct gaaaaattag ttgcaacgct tccccaatcc gagtatatag aacgagtgga
     8221 atacagggcg cccggattta taaatttttt ccttagtccg cgtgtgcttc agcgaatagt
     8281 tgctgaaatt cttaaagccg gtgaaaatta tggcaaactc gatattggga aaaatcagcg
     8341 cgtacaagtc gaatttgtca gcgccaatcc gactggtcca ctgactgtcg gtcatggtcg
     8401 tcaggcggtt ttaggcgata ccgtagcgcg tattttggaa tgggttggtt accgagttga
     8461 tcgcgagtat tattataacg acgccgggcg gcaaatgcgt atgctcgggc attcggttta
     8521 ttggcgttat cgccaattgt tgggttggaa gggcgaattt cccgaggacc attatcaagg
     8581 cgaatatatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tggtttcggg
     8701 cgggtcttta tggaggcacc gacgaccgcg ttctctggaa aagcgtcgta gatgaagcta
     8761 cctatcgttt gccggatatc gcctatcacg tcactaaatt tgaacgcggc tacgatttga
     8821 taattgacat tttcggagcc gatcatcacg ctacttatcc ggatgttctg gctggtttgc
     8881 gcgccttggg ctatgatact tcgcgagtta aagtcctgat tcatcagttt gtgacattga
     8941 ttaaaggcgg tgaaaaagta aagatgtcaa cccgcaaggc gaactatgtc acgcttgatg
     9001 aattgattga cgatgttggg cgggatgtag cgcgttattt ctttctaatg cgcggtatga
     9061 agagccattt gaattttgat ctcgatttgg caaaaaccga gagtgaagaa aatccggttt
     9121 tttatttgca atatgcccat gctcgaattt gcagtatttt gcggaatgcg gctacgaaaa
     9181 atcttgccgt aggaaatgac tttgatatga ctttattgaa ggaagaagaa accttgagtt
     9241 tgctcaatgc gctcagcgaa ttcaaagaag tcattgaaat ttgtgttagc actctcgagc
     9301 cccaacatct aacaacttat ttacaaaaag ttgccactgc tttccataaa ttttatacag
     9361 ttcatcgcgt catttcaaaa gattgggaat tgtcgcaggc gcgcctggcg ctggtcaaag
     9421 ccgtccaaat cgttatggct aatgggctgg cgttactggg aatttccgca ccggaacgta
     9481 tgtagtaaaa ttttcatcgg cgaacaaaaa ctaagccaaa cagaattcca gtgcttgatt
     9541 taagcaggta aaatctttaa attgctcggc gaaattagaa gtatgggctg taaaaatgcg
     9601 agttgatcta aaaacggcgg agaaaatagc tgatttggcg cgcttgacct tgagcgaggc
     9661 ggaactcgag cgatatgccg ctcagatgga tcagatttta gagtacattg agcagttgca
     9721 aaacctggat acaagtaatg tcaagccgct atctcatgtc caagaaataa ctaacgtttt
     9781 tcgaaatgat gatgtcaaaa cgtctctacc caaagaggcg gtttttgcta atgccccaca
     9841 agaaaaagcc ggatacttcg ttgttcctaa agtcatcaag gacattaatc catgaaagtg
     9901 ctgggggtca ttcccgcacg ttttgcggct acccgtctgc ccggcaagcc gctggttagc
     9961 ctccacggaa aaacgcttat ccagagagta tatgaaaatg cccgtaattc tcaattgatt
    10021 aatcaattag ttgtggcaac cgatgatcaa cgtatattcg aacatgttaa atcgttcggc
    10081 ggggaatgcc ggatgactcc ggttgaattg gcttccggta ccgaccgttg tgctcttgtg
    10141 gcgcgggata acgaagctga tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnntcacgtg gttttaaggt aaccattcag aaattcgatc cgtatattaa cattgatccc
    10321 ggaacgatga gcccattcca gcatggcgag gtttttgtcc tggaagatgg ttccgagact
    10381 gacctcgatc tgggccatta tgagcgtttc attgatgaaa atatgaacac gcgcaatagc
    10441 aatacttcag gaaaaattta ctatgaagtg attaatcgcg aacggcgtgg tgattatctg
    10501 ggagcgacgg tacaggttat tccgcatatc acggatgaaa ttaaacgccg gataacggct
    10561 gttaatgtcg attctgctta tgacgttgtt atttgcgaaa taggtggcac ggtcggcgat
    10621 attgaaagtc agccgtttct ggaagctatt cgccagtttc gtttcgaatc agggcgtggc
    10681 aattgcatca atattcacct gactctcgtg ccttatatcg aaaccagtgg agaaataaaa
    10741 accaaaccga cacagcatag tgttatgcgt ctgcgcgaga ttggtattca gcctgatatt
    10801 ctgctatgtc gctcgaagtt tcatctcgat gaaagcattc ataaaaagat agccctattc
    10861 tgtaatgtta tgccggaagt ggtcattgat accattgatg ccgaaacaat ttatgagatt
    10921 ccactgattt ttgatcaaca gaagttgggt gatcagatta ccgatctgtt agatttaccg
    10981 cgccggaaac cagacctcaa agatttggag aagtttgttc aggcgataaa aaatccaaag
    11041 gacgaggtca acattgcgat ttgtgggaaa tatacccagt tgcacgatgc ctataaatca
    11101 attatcgaat cattcaacca tgccggtgcg gagaataata cgcgcgttaa tattgtctgg
    11161 attgaatcgg aagacttgac caatgactgc gaaactgtca ataaactaaa aaatgttgat
    11221 ggcattctgg ttccgggtgg ttttggcgaa cgcggtgtca aaggcaagat taatgcgatt
    11281 aagttagctc gcgaaaatgg cattccgttt ctcggtattt gtcttgggct acaatgtgct
    11341 gtagttgaat ttgctcgtaa tgtatgcggc tggaaggacg ccgatagtac ggaatttaac
    11401 gaaaataccc agcatccggt cattgacctt atggaggagc agaagcgcgt tttcaataaa
    11461 ggcggtacga tgcggctcgg ggcttatacc gccgaagtca ttcccggtac tttagcctat
    11521 gaaatttata aaaataagca aatttccgag cgtcatcgtc accgctacga agtcaataat
    11581 acttatttgg ctgaaatggt caaagccggt ttggtcgtcg gtgcggtcaa taaagaactg
    11641 aatctggttg agatgattga gttgaaaaat catccctggt ttgttgcttg tcaatttcat
    11701 ccggaaatga aatcacggat taataaagcc cacccgattt ttcgtgaatt tgtgcgcgcg
    11761 gcgcggaaat atcataacca aaaagccgaa aatgccagaa gtcaaagtta aaaatatagc
    11821 catcggcaac catcaaccat tggcttttat tcttggaccc tgtgtaatcg aaagccgc
//
LOCUS       DAOMJU010000064        11795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5511_length_11795_cov_17.947956, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000064 DAOMJU010000000
VERSION     DAOMJU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11795
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5511_length_11795_cov_17.947956"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PLO58_06910"
     CDS             complement(<1..1193)
                     /locus_tag="PLO58_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74555.1"
                     /translation="MKQKLTLLAIVTIFQISLAQNFTYILCEGNFNTINASLWQLTGT
                     SELIGPVHWNFTDNPLGDVGQNLKVYQEKLFIVMNNSNSLEVMSLSNGATYERTIDLP
                     FAGPRDIAFVNNHAYITCWYLPGILSLDLNTWQIDDTIFINGLPDNIIADDGKLYTSI
                     AMNTDWSPADKVLALESVSGEFTPADTFTVITGPNQLLLYENKLFVLSTYYDDTWNTY
                     TGTSCINLETNQVTKREQGLSFNFCDDLALINDKIYRSYNGGVAPLADSLTIDTNKII
                     GNYAGIYSMAFDGKNIFIGLSDYSAPDQVVILDTLGNEINTLNVGVCPGSFAFYQQPN
                     AEIFTDEKNGILSDFLLMTNYPNPFNSNTIIPFYLKNTNLVQLTIYNLKGELIAKLTD
                     DYFTSG"
     gene            complement(1212..3278)
                     /locus_tag="PLO58_06915"
     CDS             complement(1212..3278)
                     /locus_tag="PLO58_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO74556.1"
                     /translation="MYHSLLKIYRFFPLWLLPIALEAMEFSSGFICDQNNQPIPYVFI
                     YNKTKDNFIVSEENGVFILPASYQAGDSLLFTRIGYGEQEIVLGNLYEPVKIILPFTP
                     VNLDSVTVAGQIFPRQLSGINFSRVERTEDLGTVEHRRLWTTIPGLYLKSYGGPTGIS
                     TLSLDGSPSTHTRVVIAGFDLTSAQNGQMDISQLPPTFISSINYSPEQAGNTSAENSE
                     GLLNIEPNMSPTGFTCSAGSYGKLNLSANLDIRKTVWNSNILLGRNYYRGDYEVTWRD
                     QNFQRSNNSLQQNYFSWQSGYVFRSKAFARLIAFYSEQERGVAGQVWNPSPDAFRQDA
                     LGFIGLKIGWTVKTGHSYIQTLHRHSDERYVNPTIAINSRHVVNTDQIIYHANRSILN
                     FNLDFTSDLKSDRLQSKQAGHHSRITLTNILALPWNFHPNYELRPWVKFEHSPDLYQK
                     ATWGGRIAYRGSGILNTISGSYGQYYAYPTFNDLYWQPGGNPNLKPDETTKYEIDLIL
                     QILPELKLVLNSYYKKDKNLIQWTPVQSYWQPSNISQAERKGIKVILNWSLPNLPVDG
                     FLQGILTHSCNLTKGNNYQKPLRYTPKESYAAGLNFNFKHIVFHSTIEYVGARIAMYN
                     WPEDIILAEYLVLNANLAYKVNTTIGRFTIVAGCDNITDESFESMQGYPEPGRAFTGT
                     IEYNPK"
     regulatory      complement(3308..3504)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3612..4640)
                     /gene="add"
                     /locus_tag="PLO58_06920"
     CDS             complement(3612..4640)
                     /gene="add"
                     /locus_tag="PLO58_06920"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HQO74557.1"
                     /translation="MNKNNTGEIIQAMPKIELHLHLEGAFPLESLLRLIQKYGSNEVH
                     NIEELRQKFVFTDFAHFIETWYWKSDFYREPSDIEDMAYSTIASLSQQNIIYAEVYFS
                     PFDFIKPQMPFPVVTEAVISGVRRAESEYPIRIGLIADIVRNHGHETALARLDKITQY
                     QNEVLGIGLGGSEKEFPAKLFAEVYTEAQKRGFRLVAHAGEADGPNSIWDALRLLHAE
                     RIGHGVRAVEDPDLMEYLRLNQIPLEVCVTSNLKTKIFNSAAEHPVRRLFDYGLLITI
                     NSDDPTMFGSNLTDEFNFIANEYNFTLPEIKKLTQNAIQASFAEDSFKQQLTLKNNEY
                     WNSIGVKE"
     gene            4777..>5018
                     /locus_tag="PLO58_06925"
     CDS             4777..>5018
                     /locus_tag="PLO58_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQO74558.1"
                     /translation="MHLTILGSGSFLPNEKRNCSGYLLDIGDETLLLDGGSGTLRQIV
                     NAERSVLEVGRVFYSHFHLDHIADFLPLLFTRKYYKP"
     assembly_gap    5019..5118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5336..6622
                     /locus_tag="PLO58_06930"
     CDS             5336..6622
                     /locus_tag="PLO58_06930"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HQO74559.1"
                     /translation="MKKLLLILMLTIYFVFFTQRIEAQNELWDRIFVFPENYRIYTLS
                     FSDSLNGWAGGETIGPVGGVILNTKDGGQTWSEQFVYPEYSWFNNIDFVDSLNGWAAG
                     LHCLFKTSNGGKEWIRVADSLFDFDIYMGLSDLQSVSFADTAFGIITGTESTIAITLD
                     GGNTWNCATINPENVFIDTVYYGVVLSAGKACVTGRGGVAVSADSGKTWHITHNEDRS
                     YIKCSFIENGYGWVLSRDGQIQKTRDYGESWIFCKQIFEDGVDYAVDISFLDSLTGWV
                     IANNGEIWETNDGGYNWLSSTISQDTALAVITSATNNATFVLNKANSLYKYKRFSAIT
                     EFFKKLPDCYKLCHNYPNPFNSSTNIKYSIPSKSPVKIQVIDVNGNLLETLVDRSHEP
                     GNYQITWDASGFSSGIYFYRIQAGDFIEARKCLLIK"
     gene            6845..8338
                     /locus_tag="PLO58_06935"
     CDS             6845..8338
                     /locus_tag="PLO58_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HQO74560.1"
                     /translation="MFNITDLIVVVTSVFCLLATAESQEYSGEKYKLDARKDTIWTKG
                     IPVEYSDFPEAEKIAVAVMELDANGVTSVEAKALTDRLRIELFNAGIFQVMERDKMNK
                     ILDEMQFQMSGCTSDECVVEVGKIVGVKKIIAGSVSKVGEIYSVSIRLIDVATSRIEA
                     TAIEDIEGALGAVLTEAIPSVARQISGLERQTKELKGKKTAFKISTEPYGASVFIDDV
                     YYGIAPIEVEVVPSVTHRLTIVNEGYDKWEHYYDIKKDQVLEINVALSKKSQPVAKNV
                     PEKPKRRFKAGLKIRYSDFHNVDKINNQVQIMNAKMAEGYELFHRRQPGAYVFDEIKN
                     FSGIELSNLRQSDEVVGFDFGLGIYRSSFTQWIKNFDATYSEPTLIFWMPSVNLNLRL
                     APIRFPLFYPFCNVGFGYDVLIIDAVEDNKSMGSPIFHSWGLMYGVGAELRLFKFIGL
                     ACDWNHYNMKYKLMNIENKATQRFKDASISQMDLSGNTVGLSLNLYY"
     gene            8404..9663
                     /locus_tag="PLO58_06940"
     CDS             8404..9663
                     /locus_tag="PLO58_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQO74561.1"
                     /translation="MVLVTIEIIILVILLGLSGFFSSSETALFSLSKIQREKIQESDP
                     AQGKIINDLLNTPRSLIITILLGNEFVNICFSSLFTLIVIQIAGTVLPWANYLIALPL
                     VLLIGEITPKTLAIKNNERLSLKFARPLSLVMVIFTPLRWFIRNISDRIVNLIVSKRD
                     QRENILTEDVIRSILEDSEKEGVIDSDESEMISNVFDFGDLIAADVMTPRSLIFSLPV
                     DMALPEVITAIKNRRISKVPIYEGNPDNIIGVLFATDLIGISLNTSNDETKPINLRNL
                     LRPPFFVPKTKRVDELFHIFQRKRISMAIVRDEDGIVQGLITIEDLLEEIFGEISDEF
                     EIKDEQFEKINDRLYRVRGTLPIVSFNELLNARIPVDEFETIGGFVSSLFDELPTVNA
                     QKIYDGWLFTVEKIENNRIEILRVKRL"
     gene            9660..10913
                     /locus_tag="PLO58_06945"
     CDS             9660..10913
                     /locus_tag="PLO58_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQO74562.1"
                     /translation="MIPTGLAILIIAICVLGEGFFSGSEIALVSVDRIRLRHDAKMGQ
                     KDAKLVINLLKRPEWVLGTTLIGTNICTVTSTMLATAIAYRYFQGAGVFVAIFCMSLI
                     NWIFAEIVPKSVFQQYADVIAPKIAPVLRIMSLIFTPLVRVFSGAASYIATILGGELS
                     HKLPFISKEELKILMKISDKSDVNPNEREMIGRLLSFKEKKAEDVMVPLIQVTALPED
                     ASVPEAARIISESKHRRLPVYRERVDNIIGILNSFDVIGENNNTPIRRFIRPAFYAPP
                     NMSINVLFDQMKKSGNNMAIIVDEYGGAEGIVTIEDILEEVVGKLEDEYDQVRPRYKI
                     KKDGTIVVSGKMEIQELNDKFGLNLPEGDYDTIGGLIIAHLQKIPRPGERLILPSVAL
                     TVTRASQRVIHEVAIRKNGSTEVTN"
     gene            complement(10917..11705)
                     /locus_tag="PLO58_06950"
     CDS             complement(10917..11705)
                     /locus_tag="PLO58_06950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQO74563.1"
                     /translation="MDDLQLQQKLSATALNAALEAGKILKQKVNTALKIDYKGRVNLI
                     TEADRMSENSIIESIRRQFPEHQILAEESPLIETSSDYKWIIDPLDGTTNFVHGFAFF
                     SVSIGLEYQKELLLGVVHAPLLGETFTAMKNQGAFLNGQPIRVSRVRKLADSLLATGF
                     PYENNATFRRNFEHFRRIYPLTQGVRRVGSAAIDLCYTACGRFDGFWELDLNAWDVAA
                     GALILQEAGGKTVNMDGSAFSIYARQILATNGLIEAELLPIFKE"
BASE COUNT         3413 a         2172 c         2579 g         3531 t
ORIGIN      
        1 ccagaggtga aataatcgtc ggtcaatttg gcgattagct cgcctttgag attatagatt
       61 gtgagctgga cgagatttgt gttcttaagg taaaagggga taatcgtgtt gctattgaat
      121 ggattagggt aatttgtcat cagtaaaaaa tcagataaaa taccattttt ttcatcagta
      181 aatatttcgg catttggttg ttgataaaaa gcaaaagaac cagggcagac gcctacattt
      241 agagtattaa tttcgttacc gagcgtatcg agaatcacca cctgatctgg tgcggaataa
      301 tcactcagtc cgatgaaaat attttttccg tcgaaagcca ttgaatagat accagcataa
      361 ttgccaatga ttttatttgt atcaatcgta agcgaatcag ccaacggtgc cacaccgccg
      421 ttataactgc gatatatttt atcgtttatt agagcaaggt cgtcacaaaa attgaacgat
      481 agcccctgct cccgcttggt cacttgattt gtttcgagat taatgcagga agttcccgta
      541 taggtattcc aggtatcgtc atagtaagta ctgaggacaa agagcttatt ttcgtataat
      601 aaaagctgat tcggaccggt gatgacagtg aaagtatccg cgggcgtaaa ttcgccggag
      661 accgattcca gagcgagcac tttatcggcc ggcgaccagt cggtattcat cgcaattgag
      721 gtatataatt ttccatcatc tgcaatgata ttatctggta agccattgat aaaaatcgta
      781 tcgtcaattt gccaggtatt taaatccagc gagagtatgc caggtaaata ccagcaggtg
      841 atgtaagcgt gattgttcac gaaggcgata tcacgcggtc cggcaaaggg caaatcgatc
      901 gtccgctcgt aagttgcacc attcgataaa ctcataactt ccaacgagtt ggaattattc
      961 atgacgatga agagcttttc ctgataaact tttaggtttt ggccgacgtc gccgagcgga
     1021 ttatcggtaa agttccaatg gacaggacca attagctcac tcgtgccggt taattgccag
     1081 agcgaggcat taatcgtatt aaaatttcct tcgcatagaa tgtaagtgaa gttctgcgcc
     1141 agtgagattt gaaaaatcgt tacaatggct aacaaggtca gtttctgttt cattttttag
     1201 tcctctcttt tttattttgg gttatactcg attgttccgg taaaagcccg tccgggttct
     1261 gggtagcctt gcatactttc gaatgattcg tcggtgatat tgtcgcatcc agcgacgatg
     1321 gtgaaacgtc cgattgtcgt attaacttta taagctaaat tggcattcag aacgagatat
     1381 tcggcgagga ttatgtcttc tggccagttg tacatcgcaa ttctggcgcc aacgtattcg
     1441 attgtgctat ggaaaacaat atgtttgaaa ttaaaattta gaccagcggc gtaagattcc
     1501 ttcggagtat aacgcaaagg tttttggtaa ttgttgcctt tggtcaaatt gcaagagtgg
     1561 gttagaatgc cctgtaagaa gccgtcaacc ggtaaattgg gtaatgacca gttgaggatg
     1621 actttgatgc cttttcgctc ggcttgggaa atattcgaag gttgccaata ggattgaacc
     1681 ggcgtccatt gaatcaggtt tttatccttt ttgtaatagc tatttaagac aagtttaagc
     1741 tccggaagga tttggagaat gagatcgatt tcatatttag ttgtttcatc tggtttcaga
     1801 ttgggattac cgcccggttg ccagtataaa tcattgaacg tcggataggc gtaatattgg
     1861 ccataactgc cggagatggt attgagtatt ccagaaccac gatatgcaat tctgcctccc
     1921 caagttgctt tttgatataa atccggagaa tgctcgaatt taacccaggg acgcagttca
     1981 tagttagggt ggaaattcca gggcaaagct agaatatttg tcaaagtaat acgcgaatga
     2041 tgaccagctt gtttactctg caatcggtcg gatttaaggt cggaagtaaa atcgaggtta
     2101 aaattgagga ttgaccgatt ggcatggtaa atgatttgat cagtgttaac gacgtgccgg
     2161 ctgtttattg caatagtcgg attgacatag cgttcatccg aatgacgatg taaagtctga
     2221 atgtaagaat ggccggtttt aacagtccag ccaattttca gaccgataaa tcccagcgca
     2281 tcttgccgaa aggcatctgg cgacggattc cagacttgac cggcaactcc gcgctcctgt
     2341 tctgagtaaa aagctatcag gcgcgcaaat gccttcgatc ggaaaacgta tccagactgc
     2401 cagctgaaat agttttgctg taacgaattg tttgaacgct ggaaattttg atcacgccag
     2461 gtaacttcat aatcgccgcg ataatagttg cggcctaata atatgttgga attccatacg
     2521 gttttgcgaa tgtcgagatt ggcgctcaga tttaatttgc cgtaactgcc ggctgagcaa
     2581 gtaaaaccag taggcgacat attgggttcg atatttaaca aaccttctga attttcagcg
     2641 ctggtattgc cggcttgttc gggagaataa ttaatgctcg atataaaggt cggtggcagt
     2701 tgtgagatgt ccatctgacc gttctgagcg ctggttaaat caaaaccggc gattaccact
     2761 ctggtatgag ttgacggcga accgtccagg cttaatgtgc ttatgccagt cggtccccca
     2821 taggatttaa gatataaccc gggaattgtt gtccataagc ggcgatgctc gacggttccg
     2881 aggtcttcag tgcgttcaac tcgactgaag ttaattccgg acaactgacg tggaaaaatt
     2941 tgtccggcaa cggttaccga atccagattt actggagtga aagggagaat gatttttacc
     3001 ggttcatata aatttcccag cacgatttcc tgctcgccgt agccgatacg cgtgaaaagc
     3061 agggaatcgc cggcttgata gctggcagga aggatgaata cgccgttttc ctcgctaacg
     3121 ataaaattgt ctttagtttt attataaata aacacatagg gaatgggctg attattctga
     3181 tcacaaataa aaccgctgga aaactccatt gcttccagcg caataggtaa aagccacagc
     3241 ggaaaaaatc tatagatttt gagtaatgaa tgatacatct gcctaatcct taataacccg
     3301 cgtattccag attggccaga aaaacaccag gtatcctgac ttacgcatca tccgttgttc
     3361 tgcgccttcc agatcgaatg atccgtggca taggcagaaa cgtccgcgat tacagtagcg
     3421 gggctgtgcc cgattttcac gagcttccct gttgtgcttc tctgcttaaa acgcctttaa
     3481 atcggcattt tcaatccaat taaaaagaac ggctgaaaat tattggatag cattttcaat
     3541 tgcaagataa aatttcgtga ttattttttt agcgaagggg aataattaaa tgttatcgca
     3601 acccggaaag gttattcctt tacgccaatc gaattccaat attcattatt ttttagggta
     3661 agttgctgtt taaaactatc ttccgcgaaa ctagcttgaa tcgcattttg agtcagcttc
     3721 ttaatttcag gtaaggtaaa attatattca ttcgcgataa aattaaactc atcagttagg
     3781 tttgaaccaa acatagttgg atcatctgag ttaatcgtta tcagtaagcc ataatcaaat
     3841 aagcgccgaa cgggatgttc agccgcagaa ttgaatattt ttgttttgag attgctggtg
     3901 acgcaaactt caagcggaat ttggttcaat cgcaaatatt ccattaggtc aggatcttcg
     3961 accgccctaa cgccgtggcc aatcctttcg gcatgcaaaa ggcgcaaagc atcccagatg
     4021 gaattagggc catcggcttc accagcatgg gcaactaacc ggaagccacg tttttgggcc
     4081 tcagtataaa cctcagcgaa caatttggcc ggaaattcct tctcactgcc gccgagtccg
     4141 atgccgagga cctcattttg gtattgcgtg attttatcca atctcgccag agcagtttcg
     4201 tgaccatgat tacgaacgat gtcggcaatt aatccaatcc gaatcggata ttcggattca
     4261 gctcgccgta caccggaaat tacggcttca gttacgaccg gaaaaggcat ttgcggcttg
     4321 atgaaatcga atggtgaaaa atagacttca gcgtaaataa tgttttgttg agataaactt
     4381 gcaatcgtag aataagccat atcttcaatg tcggacggct cacgataaaa atcacttttc
     4441 cagtaccagg tttcgataaa atgagcaaaa tcggtgaaaa caaatttctg tcgcaattcc
     4501 tctatattat gcacttcgtt actgccgtat ttttggatta atcgtaaaag ggattcgagc
     4561 gggaaagccc cttccagatg caggtgcagt tcgattttag gcattgcctg gattatttcc
     4621 ccagtgttat ttttattcat tcaatctcca tgttgattgg cggaaaatat aaaataattg
     4681 cgattaccac caaattattt atattgtttt caataattga tattttcaaa ttttagcgat
     4741 ggaaataaaa ctgtagactg ataaaggagg ataagtatgc atttaacgat tctgggctct
     4801 ggctcgtttt taccgaatga gaagcgaaat tgctctggct atttgctgga tattggcgat
     4861 gagactctcc tgttagacgg cggctcgggt actttgcgtc aaatcgtcaa tgccgagcgt
     4921 tcggtgctgg aagtcgggcg cgttttttat agccattttc atctcgatca tattgctgat
     4981 tttttacctt tgctttttac gcgtaaatat tataaaccnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnta tttgattagt tttaatgagg ctgtaatttc tacacgaccc
     5161 tcaccgtttt gattttatcc ttttttatta caagacagaa ttccagttat agaaaataga
     5221 cttgatatat cttcggagtt ttagtaatat gaaaatgttt attttaacag gcgataaccg
     5281 tattattcta acaatgtgaa ttaacaaatg ttttaataaa agaaaaggag gtaaaatgaa
     5341 aaagttatta ttgattttaa tgctgactat ttattttgtt ttctttactc agagaataga
     5401 ggctcaaaac gaattgtggg atagaatttt tgtttttcct gaaaattatc ggatttatac
     5461 attatcattt tctgattcat tgaatggttg ggcaggcgga gaaacaatcg gaccagtagg
     5521 aggagtaata ttaaatacta aagatggagg ccaaacctgg tcggagcagt tcgtatatcc
     5581 ggaatattcc tggtttaata atattgattt cgttgatagt ttgaatggtt gggcggctgg
     5641 gttacattgt ttatttaaaa cttctaatgg cggtaaagaa tggattagag tcgccgattc
     5701 cctgtttgat tttgacatat atatgggatt gtccgatctt caatcagttt cttttgcaga
     5761 cactgccttt ggaatcataa caggaacaga aagtacgatt gctataacct tagatggcgg
     5821 gaatacctgg aattgtgcga ccattaatcc ggaaaatgtt tttattgata ccgtttatta
     5881 tggggtagtt ctttcggctg ggaaagcttg cgtaacaggt agaggtggag ttgcagtatc
     5941 agccgattcg gggaaaacct ggcatatcac ccataacgaa gatcggagtt atatcaaatg
     6001 ttcttttatt gaaaatggtt atggttgggt attgagtcgg gacggtcaaa tacaaaaaac
     6061 aagggattat ggtgaatcct ggattttctg taaacaaatt tttgaagatg gagtagatta
     6121 tgccgttgat atttcttttc tcgattcatt gacgggatgg gttattgcga ataatgggga
     6181 aatctgggag actaatgacg gcggttataa ctggttatca agtacaattt cacaagatac
     6241 agctttggcg gtgattactt cagcaacaaa taatgccacc ttcgttttga ataaggccaa
     6301 cagcttatat aaatataaaa gatttagtgc cattactgaa tttttcaaga aactcccgga
     6361 ttgttataaa ctttgccata actaccccaa tcctttcaat tcatccacta atattaaata
     6421 ttcaataccg agcaaatcac cggttaaaat tcaggtgatt gacgtaaatg gtaatttgct
     6481 cgagacgttg gttgacaggt cacatgagcc tggcaattac caaataacct gggacgcatc
     6541 gggattttca tctggcattt atttctatcg cattcaagcc ggtgatttta tcgaagctcg
     6601 aaagtgtttg ttaatcaagt aaatattgcc aataatattc agaatataag aggcggtagg
     6661 ttataaatgt ctaaccttgg atttttagaa tcaagggata ggaaagatta aatacaattg
     6721 agaaaactcg agattcgatg aatcatagca ttaaaaagtg tgagaaatat catatctaca
     6781 aataccgatt gcggttatat tacccgaaaa ttttgaggat atacgattta tttatgatga
     6841 ggaaatgttc aatataaccg acttaattgt agtggtaaca tcggtttttt gtctattggc
     6901 aacggctgaa tctcaagaat atagtggaga aaagtataaa ttagatgctc gtaaagacac
     6961 tatttggact aaagggattc cagttgaata ttctgacttt ccagaggcgg agaaaatcgc
     7021 cgttgcagtt atggaattag atgctaatgg cgttacttcg gtggaagcaa aagcgctgac
     7081 tgatcgctta cgaatcgagc tattcaacgc cggtattttc caggttatgg aacgcgataa
     7141 gatgaataaa attctcgatg aaatgcaatt tcaaatgagt ggttgtactt ccgatgaatg
     7201 tgtcgtcgaa gtcggaaaaa tcgtaggcgt caagaaaatt atcgctggtt ccgtcagcaa
     7261 agtcggtgaa atttattcgg tgagcatccg attaattgat gtcgcaacca gccggatcga
     7321 agccacggct attgaagaca ttgagggagc acttggagca gttttgaccg aagcaattcc
     7381 atctgtagct cgtcaaattt ctggcctcga gcgtcagact aaagaattaa aaggtaaaaa
     7441 aacagccttc aaaataagca ctgaacctta cggcgcttca gtatttatag atgatgttta
     7501 ctatggaatt gctcccatcg aagttgaagt tgtccccagc gttactcata gattgaccat
     7561 tgttaacgaa ggctatgata aatgggaaca ttattacgat ataaaaaagg atcaagtact
     7621 ggaaattaac gttgctcttt ctaagaaatc ccaaccggtt gcgaaaaatg taccagaaaa
     7681 accgaagcgt cgatttaaag caggtctaaa aattcgctac tccgattttc ataacgttga
     7741 taaaatcaat aatcaagtac agataatgaa tgccaaaatg gcggaaggtt atgaactttt
     7801 tcatcgcaga caacccggag cttacgtttt tgatgagatt aagaatttct caggcatcga
     7861 attatccaat ttaaggcaat cggatgaagt agttggtttt gatttcggtc tgggtatcta
     7921 tcgcagtagt tttacccaat ggattaagaa ttttgatgct acctattcag agccgacttt
     7981 aatattctgg atgccgtcgg taaatctgaa tttgcgtttg gcgcctattc gttttccttt
     8041 attttatccg ttttgcaatg tcgggttcgg ttatgatgtg ctaatcatag atgcagttga
     8101 agataataaa tcaatgggta gtccgatttt tcacagctgg gggttaatgt acggagttgg
     8161 cgccgaactc cgcctgttca aatttattgg attagcatgc gactggaatc attataatat
     8221 gaaatacaaa ttaatgaata tagaaaacaa agccacgcaa cgctttaaag atgccagcat
     8281 ttctcaaatg gatttatccg gaaatactgt cggtctatct ctcaatctat attattagaa
     8341 gtaaattgaa gtaaaaattt tctggttggt attttatatc accgctattt attatattaa
     8401 tttgtggttt tggttacgat tgaaataatt atactggtca ttttgctggg attgtccgga
     8461 ttcttctcca gctcggagac ggctcttttt tccttgtcga aaattcaacg tgagaaaatt
     8521 caagagagcg atcccgccca gggtaaaata atcaacgatc tccttaatac accccgctcg
     8581 ctgattatta cgattcttct gggtaatgaa tttgttaata tttgtttttc atctttgttt
     8641 actctgattg ttatccagat tgccggaacg gttttgccgt gggcaaatta tttgattgct
     8701 ttgccgctag ttctgctgat aggtgaaata acgcctaaga ccttggctat caagaataac
     8761 gaacgtttgt ccctaaaatt tgcccgaccg ctttcgctgg ttatggtaat ttttacgcca
     8821 ttacgctggt ttatcaggaa tatttccgac cgaatcgtta atctgatcgt ttcaaaaaga
     8881 gatcagcgcg aaaatatttt gactgaagat gttattcgct caatattgga agacagtgaa
     8941 aaagaaggcg ttattgattc cgatgagagt gaaatgattt ctaatgtatt cgattttggc
     9001 gatttgattg ccgccgatgt tatgacaccc cgcagtttaa tttttagtct gccggttgat
     9061 atggcgctac cggaagttat caccgccatc aagaataggc gtatatctaa agtcccgatt
     9121 tatgaaggca atcctgacaa tattatcgga gtattatttg ctactgattt aataggcatt
     9181 tcgctgaata ccagtaacga tgaaactaag ccgataaatc ttcggaatct tctgcgccca
     9241 ccgttttttg tacccaaaac caaacgagtg gatgaactgt tccatatttt tcaaagaaag
     9301 cgaatttcaa tggctattgt tcgggatgaa gacggcatag tgcaaggctt gattaccatt
     9361 gaggatttac tggaagaaat tttcggtgaa atttcggatg aattcgaaat aaaagatgag
     9421 caatttgaga aaattaacga tcggctctat cgagtgcgtg gtactctgcc catagttagt
     9481 tttaatgaat tattgaacgc cagaattccg gtagatgaat ttgagactat cggtggattt
     9541 gtatccagtt tatttgatga attacctact gttaatgctc aaaagattta cgatggctgg
     9601 ctattcaccg ttgagaaaat agaaaataac cgtatcgaaa tcttgcgggt gaagcgattg
     9661 tgataccgac cggtttggca attctaatta tagctatttg cgtgctcggg gaagggtttt
     9721 tctccggttc ggaaattgca ctggtatccg tggatcgaat ccgtctccgc cacgatgcca
     9781 aaatgggcca aaaagatgct aagttagtca taaatctttt aaaacgaccg gaatgggttt
     9841 tggggacgac cttgatcggc acgaatattt gcacagtgac cagcacgatg ctggcaacgg
     9901 ctatagccta tagatatttt cagggcgccg gagtattcgt cgcaattttt tgtatgagtt
     9961 tgatcaattg gatttttgct gaaatcgttc ctaaaagcgt tttccagcaa tatgccgatg
    10021 taattgctcc gaaaatagcg cctgtattga ggataatgtc tcttatattt acaccattgg
    10081 ttcgtgtttt ttcgggagcc gcttcttata tcgctaccat tctcggcggc gaactcagcc
    10141 acaaattacc atttatcagt aaagaagagc tgaaaatcct aatgaaaata agcgataaga
    10201 gtgacgttaa tcctaatgaa cgtgaaatga tcggccgact tttatcattt aaagaaaaaa
    10261 aggctgaaga tgtaatggtg ccgctcattc aagttactgc tctgcctgag gacgccagcg
    10321 ttccggaagc ggcgcgcatc atttccgaaa gtaagcatcg tcgtctgccg gtgtatcgtg
    10381 aacgggttga caatatcatt ggcatattga atagtttcga tgtcatcggt gaaaataaca
    10441 atactccaat caggagattt atacgtccgg ctttttatgc accacccaat atgagcataa
    10501 acgttttgtt cgatcaaatg aaaaaaagcg gcaacaatat ggcaataatt gttgacgaat
    10561 atggcggcgc ggagggcata gtgactattg aggacattct agaagaggtt gtcggcaaat
    10621 tagaagatga atatgatcag gtccgtcctc gctataaaat taaaaaggat gggacgattg
    10681 ttgtctctgg caaaatggaa attcaggaac tcaatgacaa atttggactg aatttgccag
    10741 aaggtgatta cgatactatt ggcgggctca ttattgctca tctgcaaaaa attccgcgtc
    10801 ccggagaaag attaattctg ccatctgttg cattgacggt aacacgcgct tctcaacgcg
    10861 tgattcatga ggtcgccatt cggaaaaatg gttcgactga agtaactaat tagaatttat
    10921 tctttgaaaa tcggcaataa ttcggcttcg attaaaccat tcgtcgctaa aatctggcgg
    10981 gcataaatcg aaaatgccga accgtccata tttaccgttt tgccaccggc ttcttggaga
    11041 attagcgctc cagcggctac gtcccaggcg tttaaatcca actcccagaa tccatcaaag
    11101 cggccacagg cagtataaca caaatcaatt gccgccgaac cgacacgccg aacaccctga
    11161 gtaagcgggt aaatgcgccg gaaatgctcg aaattgcgtc ggaaagtggc gttattttcg
    11221 taagggaaac cggtagcaag aagactatca gcaagttttc ttacccgcga aactcgaata
    11281 ggttgtccgt taagaaaggc gccctgattt ttcatggcgg taaaagtttc gcccagcaaa
    11341 ggagcgtgga caacgcctaa taacaattct ttttgatatt ccagtccaat tgaaacagaa
    11401 aagaatgcaa aaccgtgcac aaaattggtt gtaccatcca gcggatctat gatccacttg
    11461 taatcagaag aagtttctat cagcggactt tcttccgcca gaatctgatg ctcgggaaat
    11521 tgacggcgga ttgactcgat aatagaattt tccgacatac gatcagcttc ggtaattaga
    11581 ttgacacgtc ctttatagtc aattttcaag gcggtattga ctttttgttt gagaattttt
    11641 cccgcctcga gcgcggcgtt taaagccgtc gctgataatt tctgttgcaa ttgtaaatcg
    11701 tccatcatta tttccttaat ttcattgatg ttatagttaa ttaatccaat ttttagacta
    11761 agtgtagttt gttttaatta ttaaaggtcg caaat
//
LOCUS       DAOMJU010000065        11737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5556_length_11737_cov_17.846602, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000065 DAOMJU010000000
VERSION     DAOMJU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5556_length_11737_cov_17.846602"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLO58_06955"
     CDS             complement(<1..608)
                     /locus_tag="PLO58_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQO74564.1"
                     /translation="MPNLNSIEIKKRAIELYKLLDPCQICPRLCRAKRLHGAAGFCQV
                     LDKAILSSALAHFGEEPPITGRGGSGTIFLSSCNAACIFCQNYQISHERIGNPVSISQ
                     LANEYLHLQAVGCENINWVTPTPHLPFLVDALASAVEQGLKIPVVYNTNGYDRVEVLR
                     LLDGIVDIYLPDMKYGEDVWAEEYSGLKNYFTINVAAIKEMYR"
     gene            complement(682..2055)
                     /locus_tag="PLO58_06960"
     CDS             complement(682..2055)
                     /locus_tag="PLO58_06960"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HQO74565.1"
                     /translation="MNKLRYWIITLILNVWCFSATGPCFDDYFLDQTMRLDFYFTGTK
                     TQNIFSLDAVYISPLWSGSQTNLIDTLNLGHHLIRVYDADSQTLIYSRGFSSIFNEWQ
                     STNEAIQEIWRTFHFSVLIPLPRQAITVTLSSRDRQNQFIEYFKTNIEPDSRFINRDG
                     VHTDYRLKKLFYNGSPTQKIDLLILPEGYTKSEMRQFRQKCRHFTEVLFNVSPFKEES
                     DKFNVWFLEVPSAESGIDNPRGGTFKRTAFDLTYNALDLDRYVLTLNNKTVRDIAAQA
                     PYDFIIFIFNSDKYGGGGIFNLYSTCYSTAAKPEQKGWPEYVFVHEFGHSFAGLADEY
                     YTSSTPYNELYPQGVEPWEPNITALLNPTALKWLDLVSPEVPVPTPWEKSAYEQATYS
                     KASEFEQFLKNQKYYGKVGAFQGAGYASEGLYRPCLDCIMFSRNEIAFCPVCQAAIRK
                     IIHFYSE"
     gene            2228..2887
                     /gene="ftsE"
                     /locus_tag="PLO58_06965"
     CDS             2228..2887
                     /gene="ftsE"
                     /locus_tag="PLO58_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HQO74566.1"
                     /translation="MITFFNVSCTFGKDAGVKDVNLTINEGEFVFLTGSSGAGKSTLL
                     RLIYMDLFPEKGKVVIRNFDSSKVRPNEIPFLRRKVGMIFQDFRLLEDRNVYENVALS
                     LYVSHYKTREIKNRVYNVLNQVGLGHRVYYNPRELSGGEQQRVAIARALVKEPVVLLA
                     DEPTGNLDPRVASDLMNLLIKINQTGTAVIVATHNYSLIPRVANARIIQVNEGEIINP
                     V"
     gene            2884..3750
                     /locus_tag="PLO58_06970"
     CDS             2884..3750
                     /locus_tag="PLO58_06970"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HQO74567.1"
                     /translation="MTILWFLIKEGFRGLGRSGYAGAFSIIIIALSLILIGFGYVAAR
                     DTLVMVENIRSQFDITIFVHRNASLTDLENFIEELSYFEEIEKIDYISTDSAAAQFRA
                     EFGEDIFSILDYNPLPPSFTLRLKPQARNLASIEDLTTRIKQYPIVDEVRYRENFLRL
                     LEKYQRLIFIAVVTAFIGLTLISIILASNSIKMTIFSRRNVIATMKLVGATNNFVRAP
                     FLIEGTLEGLIGALLASGVIGLISYFFNNYLQGLTGYQIIVSYRYYIGLLVLGSLIGL
                     IGSMRAIRRFLE"
     gene            4051..4605
                     /gene="grpE"
                     /locus_tag="PLO58_06975"
     CDS             4051..4605
                     /gene="grpE"
                     /locus_tag="PLO58_06975"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQO74568.1"
                     /translation="MEDISTNERTTGINKNQTRSTKKQTSEKERLSAKIAELEAKVAE
                     YEKALNEANEKFLRLAAEFDNYRKRREKEYSDIIEYAGEEVLRNILPIMDDLERALNN
                     INDDQSLREGLELIYRKFQKTLQNLGVEPIESVNKPFDPNLHHAIMTREEAGIAPDIV
                     LEEYEKGYKFKNRVLRYAKVVVSK"
     gene            4607..5761
                     /gene="dnaJ"
                     /locus_tag="PLO58_06980"
     CDS             4607..5761
                     /gene="dnaJ"
                     /locus_tag="PLO58_06980"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQO74569.1"
                     /translation="MAKDYYEILGVNRNAQADELKKAYRKIAMKYHPDRNPGNKEAEE
                     KFKEAAEAYAVLSDPEKRRVYDQYGEDGLKGTTGFSGAQSMNMEDIFSMFGDIFGGHF
                     GGFGGFDDLFNGGGRQRSTRTRTRTTQQRGSDLKITMPLTLEEINTGVDKKVKIKRQE
                     ICPNCNGTGAKPGSSNVTCPVCQGSGEVREVSRSFFGQMVNVRPCSNCQGEGVIVEQR
                     CPQCGGEGRIQSVKELTIKVPPGVTTGNYLTMHGEGNAGVRKSSRGDLIVIFEEKPHE
                     YFTREGDDIYLDLHLMPSEAVLGTEIVVPTLSGKVSLTIPPGTQPSKILRLKNKGLPH
                     LQHPGRGDMMVRIQVKIPQRVSGKELELYRELFKLESKKIKDENRYSKIR"
     gene            5845..6255
                     /locus_tag="PLO58_06985"
     CDS             5845..6255
                     /locus_tag="PLO58_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQO74570.1"
                     /translation="MVIQLLVACFLVGATIRIYKMRSNRINNEESRQQTVATDSFQLY
                     ASQIDSEYTELQRSGHSTYQSQKSTALQQKINLNTATKQELMLLPKIGPVTADHILEY
                     RAQVGKFNRIEELLNVKGIGTKTFEKIKGEVTLE"
     gene            6248..7030
                     /locus_tag="PLO58_06990"
     CDS             6248..7030
                     /locus_tag="PLO58_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74571.1"
                     /translation="MSDETAKFNQEEEIIPEYKPTLVKVLNVVAFILIIVLIAVIYIP
                     SSIWKEENRIRELGRQRMQIQYDVQKYYNQMAGTHQPDPVLAMKVVSAVRDSTRADSN
                     FYGQKVIKLPDGKFNLEVPKNFYASFDTNFAFKYELSDTVIDTTIKITKWNPELMTID
                     TLYVISSMLEEVKTDPNFRSVIDTELTQRVANNTYYRRYYLTEDLAMRPLLDTPYTIL
                     KTEKGIRVEDPLKTVIREPRYLFFTFVDSSHGYIENGEPSWK"
     gene            7055..7999
                     /locus_tag="PLO58_06995"
     CDS             7055..7999
                     /locus_tag="PLO58_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74572.1"
                     /translation="MLAISFSDNFIRYGQLIKTDNSISIELIGKKKLPFKFDTTSLKN
                     PDLIPSIESLLLNIKNRLPVQERFLALSAPSQLFDITINSIDLGLEKQKIAEILDWNE
                     KQRLGVVFNKKFVQHYPLKQRISENKRDYLTISYFKELGRAIYQACQRLGFIIKVFDL
                     NIFSAANALESLYKEKNGKKWGLWLIGEKYHILLLIKSGEISQYLEFDLNNDGDYNIL
                     VDSNPDEDGKEIVSQINNLRNLSSETIQDIENLYFFTYDVNSEFYNMLLTYDIDNLRC
                     VDPFENFKPVKIYQDDGSGPGAMSQFLDVMGLLYRFML"
     gene            8008..9192
                     /locus_tag="PLO58_07000"
     CDS             8008..9192
                     /locus_tag="PLO58_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74573.1"
                     /translation="MIKINLAATPGLQQEPKDIAISQSLKDILIKMAVEPKNQPIDDQ
                     AISESSTKPIPEKVEKKTNVFASQGSNSSIPGGTDAEEPIPQPPPKISDKLREAEQII
                     ERKEEEYSKHLFNRPILNIGIWILVLVIVGALGYWGYTKYFNPRKSAITAKPAGKSDL
                     PVEQPILLTEQSQPSSTSDMPEVDQPVLTPINQIDEIYLPPVLTGISRCQILSQLISV
                     LPDNSRIQFLKLDKDVVNFLIFVDTENEAQQVKAEVQKLPGIGSIQAFYIEHVADTPS
                     PAVQMMAILNLKTESAVARNIRFYDDVSLSQVLWAIGKRQNLKLQPLNIAEPPAPKPR
                     LAEIRGNGSLNSIINLLSELSNFDLNLSVEKIAINLSADSAVDTGILDFSINSVIYPQ
                     KL"
     gene            9195..9719
                     /gene="rsmD"
                     /locus_tag="PLO58_07005"
     CDS             9195..9719
                     /gene="rsmD"
                     /locus_tag="PLO58_07005"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HQO74574.1"
                     /translation="MVRVIAGLYKNQSIMSDKKATIRPTQDRVKAALFSILGDVSGLK
                     VADLFAGTGNLGIEALSRGAERCTFVDNNWQSLNLVKHNLAKLKLEQRAEVIQADVLK
                     YLAQKPLFDLIFADPPYEYSSYNELLMLFGQLRPDTQIVLETDRRFDLPSSFKPREIK
                     IRRSGDTNLNFIRI"
     gene            9720..10199
                     /gene="coaD"
                     /locus_tag="PLO58_07010"
     CDS             9720..10199
                     /gene="coaD"
                     /locus_tag="PLO58_07010"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQO74575.1"
                     /translation="MKIAVYPGSFDPITNGHLDILKRASSLFDLVYIAVARNLQKQPI
                     FTTEERLALIRGCTSEFENVKMDSFDGLVVEYARQVGAEVIIRGLRALSDFDYEFQMA
                     LMNRHLDENINTVFLMPHEDYTYLSSSMVRDLAKFGAEIGSFVPANVKAALLKKFRE"
     gene            10201..11178
                     /gene="pta"
                     /locus_tag="PLO58_07015"
     CDS             10201..11178
                     /gene="pta"
                     /locus_tag="PLO58_07015"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HQO74576.1"
                     /translation="MKLIEEIRSQAQKCKKTVVLPEAHDERILKAAEILEKEGLAQII
                     LVGDPEIIGQHAREINVNLGSIGIFDPVQFEKLDDFINTYYEKRKHKGMTLENARTVM
                     LNPTFFGAMLVEKGLADGCVSGADTTTGDVLKAALHIIGTNPDTPTVSSDILIITPDE
                     EKIWSFADCAVLPNPTAEQLADIAIATANTHRRVVKTEPIVAMLSFSTKGSAKHELVD
                     KVIQATAIVRGKYPDLRIDGELQADAAIVPTIGAKKAPGSPVAGQANVLIFPDLNAGN
                     IGYKLVQRIGNCEAIGPIIQGLKKPMNDLSRGCSVEDVVNVTAIICNLV"
     gene            11199..11489
                     /locus_tag="PLO58_07020"
     CDS             11199..11489
                     /locus_tag="PLO58_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQO74577.1"
                     /translation="MPTYEYKCEKCGYEFEQFQKMSAEPLKVCPKCGQKSVTRKISGG
                     AGLIFKGSGFYVNDYGDKNKTKSPSPTTTEDKPTSTPKAKEKTITTSSNSSN"
     gene            complement(11540..>11737)
                     /locus_tag="PLO58_07025"
     CDS             complement(11540..>11737)
                     /locus_tag="PLO58_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009012330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HQO74578.1"
                     /translation="TKINYLLFAIGIAVIIIGYICMATGETYSFRSLTVAPIILLIGY
                     LIIIPLAILYRKKEEKTPSES"
BASE COUNT         3705 a         2172 c         2474 g         3386 t
ORIGIN      
        1 cgatacatct ctttgattgc cgctacgttt atggtaaagt agttttttaa tcccgaatat
       61 tcttctgccc agacatcctc gccgtatttc atatctggca agtatatatc aacgatacca
      121 tccagaagac gtaacacttc gacccggtcg taaccattgg tattgtaaac gacaggaatt
      181 tttaatccct gttcaaccgc cgaagccagt gcatccacca gaaatggtaa atgaggagtc
      241 ggcgtaaccc agttgatatt ttcacaaccg acagcctgga gatgtaaata ttcattagct
      301 aattgagaaa tagatacagg attgccgatc cgttcgtggc taatctggta attttggcaa
      361 aaaatacagg cggcgttgca ggatgacaga aagattgtcc cagaaccacc gcgaccggtt
      421 attggcggtt cctcgccgaa atgtgccaga gcgctggaaa gaatagcctt atccagcacc
      481 tgacaaaatc ccgccgctcc gtgtaaacgc tttgcccggc agagtcgcgg gcaaatttga
      541 cagggatcga gcagtttata aagttcaata gcccgttttt ttatttctat agaatttaag
      601 ttcggcatcg ttagcgtgtt atttcatcat ttattttaca ttttgatgtc tttacaataa
      661 tttaattata ttgaaattac tttactcgct atagaaatgg attattttcc ggattgccgc
      721 ttggcagacc gggcaaaagg caatttcatt gcgtgaaaac ataatgcagt ccaggcaagg
      781 acgatataaa ccttcggagg cgtagccagc gccctggaat gctccgactt ttccataata
      841 tttttggttt tttagaaatt gttcaaattc acttgccttg ctataagtgg cttgttcata
      901 agcggatttt tcccagggtg tcggcacagg gacttcagga gaaaccaaat ctaaccattt
      961 caatgcggtt ggattcaata gggcggtaat gttcggttcc catggctcga ctccctgcgg
     1021 gtagagttca ttataaggcg tactcgaggt gtaatattcg tccgccagac cggcgaatga
     1081 atggccaaat tcgtggacaa aaacatactc aggccagcct ttttgttctg gtttggcggc
     1141 tgtactgtag caggtagaat aaagattaaa aatgccgccg ccgccgtatt tgtccgaatt
     1201 gaaaataaaa atgataaagt cataaggagc ctgagcggca atgtcacgta cggttttatt
     1261 attgagtgtc aatacataac gatcgagatc aagagcatta taagtcaggt caaaggcggt
     1321 tcttttaaag gtaccgcctc gcggattatc aatgcccgat tcagctgaag gcacttccaa
     1381 gaaccagacg ttaaatttat cggattcctc tttaaaggga ctcacgttga ataagacctc
     1441 ggtaaaatga cggcatttct ggcgaaattg acgcatttca gactttgtat aaccttctgg
     1501 taaaattagc aggtcaattt tttgggtggg tgatccgtta tagaataact ttttcaaccg
     1561 ataatcagta tggacgccat cccgattaat aaaccgggaa tcgggctcaa tgttggtctt
     1621 aaaatactcg atgaactgat tttggcgatc acgggaagaa agcgttacgg tgatcgcttg
     1681 gcgcggtaat ggaatcagga cagaaaaatg aaaagtgcgc caaatttcct gaatagcttc
     1741 attagttgat tgccattcat taaaaatact gctaaagccg cgggagtaga tcaaagtttg
     1801 gctatccgca tcgtaaacgc gaataagatg gtgccccagg ttaagtgtgt caattaaatt
     1861 ggtctgactg cccgaccaga gaggactaat ataaaccgca tcgagactga aaatgttctg
     1921 agtcttggtt ccagtgaagt aaaaatcgag tcgcatagtt tggtcaagaa aatagtcatc
     1981 aaaacaagga ccggtcgctg aaaaacacca aacattcaaa atcaaggtaa tgatccaata
     2041 tcttaattta ttcattgaca tttctccatt ttcctattgg caaattattc aagctgactt
     2101 tagatttcaa gagaaagttt ttcggtgagt taaagtaact atgaagtata actataatat
     2161 ttagaagaaa cggcgttgat ttattatatt caggcgttta aattgtccga gttttagaac
     2221 ttaaattatg ataacttttt tcaacgttag ttgcaccttc ggtaaagatg ccggagttaa
     2281 agatgtcaat cttacgatta atgaaggaga gtttgttttt ctaactggtt cttccggcgc
     2341 tgggaaatca acgcttttac gcttgatata tatggacttg tttcccgaaa agggcaaagt
     2401 tgtcatccgt aattttgata gttctaaagt ccgaccaaat gaaatcccat ttctaagacg
     2461 caaagtcggg atgattttcc aggattttcg actactcgag gatcggaacg tttatgaaaa
     2521 tgtggctctt agtctgtatg tgtcacatta taaaactcgc gaaattaaaa atcgagtcta
     2581 taacgttttg aatcaagttg ggctgggaca tcgcgtttac tataatcccc gcgaattatc
     2641 cggtggcgag caacagcggg tcgccattgc ccgagcactg gtaaaagaac cggtagtatt
     2701 attggccgac gaaccgaccg gcaacctcga tccgcgcgtc gcttccgatt tgatgaatct
     2761 tttaattaaa attaaccaaa ctgggacggc tgtaatcgtt gccacgcata attattcact
     2821 catcccgcgg gtggcaaatg cccgcataat tcaggtaaat gaaggagaaa taattaatcc
     2881 agtatgacca tattgtggtt tttgattaaa gagggatttc gcggattagg gcgttccggc
     2941 tatgccggtg ctttttccat cataattatc gctctatcgc taattctaat tggattcgga
     3001 tacgttgccg cccgtgatac tttagtaatg gtggaaaata ttcgttcaca gttcgatata
     3061 actattttcg ttcaccgtaa tgcgtcatta accgacttgg aaaattttat tgaggaatta
     3121 agttactttg aagaaattga gaaaattgac tacatttcaa ccgatagtgc cgctgcccaa
     3181 ttcagagccg aatttggaga agatatattt agtattcttg attataatcc tctgccacct
     3241 tcatttactc ttcgtctcaa gcctcaagcc cgtaatctag ctagtattga ggatcttact
     3301 actcgtatta aacaatatcc gatcgtggat gaagtccgct atcgtgaaaa tttcctgcga
     3361 ttgctggaaa agtatcagcg cttgatattc attgccgtag tcacagcgtt tattggcttg
     3421 acgttgatct cgattatttt agcttcaaat tccattaaaa tgacgatttt ttcccgccga
     3481 aatgtcattg ctacgatgaa attagtcgga gcaacgaata atttcgtacg agcgcctttt
     3541 ttgattgaag gaacgctcga gggattgatc ggggctttat tagcttcagg cgtaatcggt
     3601 ctgatctcct attttttcaa taattattta caaggactta ctggttatca aataatcgtc
     3661 agctaccgtt attatatagg attgctggtt ttgggcagtt taatcggttt aatcggtagt
     3721 atgcgcgcta ttcgtagatt cctcgaataa gaaatacctg agaataattc ccttcaaatt
     3781 ataaataata ttactcaatt tattttggaa tgacctatca gaaaatttaa attccgcagt
     3841 tatgaatgat tttacgaaga tagaaaaata caatgagtgc ccgagtaaaa cctgtaattg
     3901 tcagaaagtc cgaaaaaatg attctccaag ccgaattatc gcatttttga ctgagcgaac
     3961 ggtcaacttc ttataactgg tattggaatt gcataattca gaacgagttt tatttaaatg
     4021 attaaaaatt aattttgagg agaatgaatc gtggaagata taagtacgaa tgaaagaaca
     4081 actggtataa ataaaaatca gactcggtca actaaaaagc aaaccagtga aaaggaaaga
     4141 ttatccgcca aaatcgcgga actggaagca aaagtagctg aatatgaaaa agccttgaac
     4201 gaagctaacg aaaaattttt gcggttagcg gcggaattcg ataattatcg taaaagacgt
     4261 gagaaggaat attcggatat aatcgagtac gctggtgaag aagttttgcg aaacattctg
     4321 ccgattatgg atgatttaga gcgggcttta aataacatca acgatgatca atctttgcgt
     4381 gaaggtctgg aacttattta tcgtaaattt caaaaaacct tgcagaattt aggtgtagaa
     4441 ccgattgagt cggtaaataa accatttgat cccaatctgc accatgcgat tatgactcgt
     4501 gaagaagcgg gaatagcgcc ggatattgtt ctggaagaat atgagaaggg gtataaattt
     4561 aaaaaccgtg ttttaagata tgccaaagtg gttgtaagta aataatatgg caaaagatta
     4621 ctatgaaatt ttaggtgtta accggaatgc acaggctgat gaattaaaga aagcctaccg
     4681 gaaaattgcc atgaaatatc atcccgatcg caatccaggg aataaagaag ctgaggaaaa
     4741 atttaaagaa gccgctgaag cttatgccgt tctatctgat ccagaaaagc gacgggtata
     4801 tgatcaatac ggtgaagacg gactaaaagg tacgactggt ttcagcgggg cacagagtat
     4861 gaatatggaa gatatttttt ccatgttcgg ggatattttt ggtggccatt tcggtggttt
     4921 tggcggattt gatgacttat tcaatggcgg tgggcggcaa cgctctaccc gtactcgaac
     4981 tcgtacgact cagcaacgcg gtagtgatct taagattaca atgccactta ctttagaaga
     5041 aatcaatacc ggcgttgata aaaaagttaa gataaaacgt caagaaatct gccccaattg
     5101 caacggtacc ggcgctaaac ccggttcttc aaatgtcacc tgtccggttt gtcaaggaag
     5161 tggcgaagtt cgggaggttt cccgttcgtt ttttggacaa atggtaaatg tgcgtccttg
     5221 ttccaattgc caaggcgaag gcgtaattgt tgaacagcgt tgtcctcagt gcgggggcga
     5281 aggacgtatc cagagtgtta aagaactgac gattaaagtt ccaccgggag ttactactgg
     5341 taattattta acgatgcacg gtgagggtaa tgctggtgtt cgcaaaagtt cgcgcggcga
     5401 ccttattgta attttcgaag aaaaaccgca tgaatatttc actcgtgaag gagatgatat
     5461 ttaccttgat cttcacctta tgccatcgga ggcagtttta gggacggaaa ttgtcgtccc
     5521 aacccttagc ggtaaagtaa gcctgactat tccgccaggc actcaaccgt ccaaaatact
     5581 gcgacttaag aataaaggct taccacattt acaacatccg ggcagaggtg acatgatggt
     5641 gagaattcag gttaaaattc ctcaaagagt cagtggcaaa gagttggaat tataccgcga
     5701 attattcaaa ttggaatcca aaaaaataaa agatgaaaat cgttatagta agattcgcta
     5761 attaatatta cattaagaga aaatgagatt tttaaagatt tttaattttt aggaaaaatg
     5821 ataaatttaa cccgccagga gaaaatggtt attcagttat tagttgcttg tttcctggta
     5881 ggtgcaacta tccgtatcta taaaatgagg tcgaaccgaa taaataatga agaaagccgc
     5941 caacagactg tagctactga ttcttttcaa ctatatgcca gtcaaattga ttcggaatat
     6001 accgagttac agagaagcgg tcattctaca tatcaatcac aaaaatcaac tgctcttcaa
     6061 caaaagataa atttaaatac cgcaaccaaa caggagctca tgttattgcc aaaaatcggt
     6121 ccggtaactg ccgaccatat tttggaatat cgtgctcaag ttggaaaatt caacaggatt
     6181 gaagaactcc taaatgtaaa aggtatcgga actaaaacgt ttgaaaaaat taaaggcgag
     6241 gtaacccttg agtgacgaaa ctgcaaaatt caaccaggaa gaagagataa tcccggaata
     6301 caaaccgaca ctagtgaaag tattaaatgt tgtggctttc attctcatca ttgttttgat
     6361 tgccgtaatt tatattccga gttcgatctg gaaagaagag aaccgaattc gggaattagg
     6421 tcgtcaaaga atgcagattc agtatgatgt ccagaaatat tataaccaga tggctggaac
     6481 tcatcaacca gacccggttc tagctatgaa ggtagtcagc gcggttcggg atagcacccg
     6541 ggctgacagc aatttttatg gtcaaaaagt gatcaaacta ccggatggca aattcaattt
     6601 ggaagtaccg aagaactttt acgcctcttt cgatactaat tttgctttta aatatgagtt
     6661 gtccgatacg gtcattgata ctactattaa aattactaag tggaatccgg aacttatgac
     6721 cattgataca ctctatgtaa tctcctcaat gcttgaagaa gtaaaaaccg atcccaattt
     6781 cagaagtgtt attgataccg aactcacgca acgggtagct aataatactt attaccgtag
     6841 atattatcta actgaggact tagctatgcg ccctctattg gatactcctt acactattct
     6901 taaaaccgaa aaaggaatta gggtggaaga ccctctcaaa actgttatcc gtgaaccgcg
     6961 ctacctcttt tttacttttg tagattcatc acacgggtat attgagaacg gtgaaccgag
     7021 ttggaagtaa atagccaaac aattcggaga taagatgctg gcaataagct tttcggataa
     7081 ttttattaga tatggccagc tgattaaaac ggacaattct atttcgatcg aattgatcgg
     7141 taaaaaaaaa ctgccgttta aatttgatac tacttcctta aaaaatcctg atctgattcc
     7201 tagcattgag tcccttttgc ttaatatcaa gaataggtta ccagtgcagg aacggttttt
     7261 agctttatca gctccctcgc aattatttga tattacgatc aattcaattg atctgggatt
     7321 ggaaaagcag aaaattgccg agattctgga ctggaacgag aaacaacgtc ttggggtagt
     7381 atttaacaaa aaatttgttc agcattatcc tctgaaacaa cgaatttctg aaaataagcg
     7441 tgattattta acaatttcat attttaagga attaggacgg gctatttacc aagcctgtca
     7501 gcggttggga tttatcataa aagtctttga tctgaatatt tttagtgcgg ctaacgcgct
     7561 tgaaagtctc tataaagaaa aaaatggaaa aaagtggggc ttatggctaa taggtgaaaa
     7621 atatcatatt ctcctactta tcaaatcggg tgaaatcagt caatatttag aatttgattt
     7681 aaataacgat ggggattata acatattggt ggattcaaat cccgacgaag atgggaaaga
     7741 gatcgtttct caaataaata atcttaggaa tttaagcagt gaaacgattc aagatattga
     7801 aaacctttat tttttcactt atgatgtcaa ttctgaattt tataatatgc ttttgactta
     7861 tgatattgat aatctacgat gtgttgatcc ttttgagaat ttcaagccgg ttaaaattta
     7921 ccaagatgat ggatcgggtc ccggagctat gagtcagttc ttggatgtaa tgggattgtt
     7981 atatcggttt atgctttagg tcgaattatg ataaaaatta atcttgccgc tacacctgga
     8041 ctacaacaag aacctaaaga tattgcgatt agtcagagcc ttaaagatat tctaatcaaa
     8101 atggcggtcg agccgaaaaa tcaaccgatt gacgatcaag caattagcga atcatcaact
     8161 aaaccgatac ctgaaaaagt tgagaaaaaa acgaatgttt ttgcttcaca aggttcaaat
     8221 tcaagtatcc ccggaggaac cgacgctgaa gaacctatcc ctcaaccgcc acctaagatt
     8281 tccgataagc tgagagaagc cgaacaaatc atcgaaagaa aagaagaaga atattccaag
     8341 catttattta accgccctat tcttaatatc ggaatctgga tattggttct ggttatagtt
     8401 ggagctttag gttattgggg atatactaag tactttaatc cacgaaaatc ggcaattacc
     8461 gctaaaccag ccggaaagtc ggatctgccg gttgaacagc caattctttt aacggaacaa
     8521 tcccagcctt cttctacatc cgatatgcca gaagttgatc agccggtttt aaccccgata
     8581 aatcagattg atgaaatata tctgccccct gttttaaccg gtatatcccg ttgtcaaata
     8641 ttatcgcagt taattagtgt tttaccagac aattcacgaa ttcaattttt aaaattagat
     8701 aaagatgtgg tcaatttctt aatttttgtt gatactgaga atgaagctca acaggttaaa
     8761 gccgaagtgc aaaaattacc gggaattgga tcaattcaag cattttatat tgaacatgtt
     8821 gcggacactc cttcgcccgc tgtccaaatg atggcaattc tcaatttaaa gactgagagt
     8881 gcggttgcta gaaatatccg tttttatgat gacgtatcac tctcgcaagt gctttgggca
     8941 atcggtaagc gtcaaaattt aaagttgcag ccgcttaata ttgccgaacc accagcgcca
     9001 aagccccgtt tggcggaaat tcgtgggaat ggctcactga acagtattat taatttatta
     9061 agtgagttat cgaattttga tttgaattta agtgttgaaa aaattgcaat aaacttaagt
     9121 gctgattcgg cagtagatac cggaatactc gatttcagta ttaattctgt aatctatcca
     9181 caaaaattgt aaagatggtt cgggtaattg ccggtttgta taaaaaccaa tccataatgt
     9241 ccgataaaaa agcgacaatt cgaccaactc aggatcgagt aaaagcggct ttattttcaa
     9301 ttttagggga tgtatccggt ctaaaagtcg ccgatttgtt tgctggaacc ggtaatttag
     9361 gaattgaggc gctatcccgc ggcgccgagc gatgcacttt cgttgataat aattggcaaa
     9421 gcctaaattt ggttaaacac aatttagcaa aactcaaatt ggaacagcgg gctgaagtta
     9481 ttcaggctga tgtcctgaaa tatttagccc aaaagccgct ttttgacctg atttttgcgg
     9541 atccaccata tgaatattcg agttataatg aactattaat gctgttcggc caattacgac
     9601 ccgatactca aattgtactc gaaacggatc gtagatttga cctgccttca tcttttaaac
     9661 cgcgggaaat aaaaattcgc agaagtggcg atactaattt gaatttcata aggatataaa
     9721 tgaaaatagc agtttatccc ggtagttttg atcctattac caatggtcat ctggatatac
     9781 tcaaacgagc gtcatcttta tttgacttag tatatattgc cgtggctcgc aatctccaga
     9841 agcaaccgat ttttaccacg gaagaaaggc tggcattgat tcgaggctgt acgagcgaat
     9901 tcgagaatgt caaaatggat agctttgatg gattagtagt cgaatatgcg cgtcaagtcg
     9961 gggcagaggt aatcatccgc ggcttacgag cattatctga tttcgattat gaatttcaga
    10021 tggcgctaat gaatcgtcat ctggatgaaa atattaacac ggtatttcta atgccccatg
    10081 aagattatac ttatctaagt tcctctatgg tgcgagattt agccaaattt ggtgccgaaa
    10141 taggtagttt tgtgccggca aatgtgaagg cggctctgtt gaaaaaattc agggagtaaa
    10201 atgaaactta tcgaggaaat taggtctcaa gcccaaaagt gtaaaaaaac ggttgtttta
    10261 ccggaagcac atgatgaacg tattttaaaa gccgccgaaa ttttggaaaa agaaggctta
    10321 gcgcaaataa ttttagttgg tgatccagag ataatcggtc aacacgcccg tgaaattaac
    10381 gtcaatctcg gctcgatagg tatatttgac cctgttcaat ttgaaaaatt ggacgatttt
    10441 attaatactt attatgaaaa acgcaagcat aaagggatga cccttgaaaa tgctcgcacc
    10501 gtaatgttaa acccgacctt tttcggagct atgctggtgg aaaaaggatt agcggacggc
    10561 tgtgtttcgg gagccgatac aaccactggc gatgttctta aagccgcatt gcatatcatc
    10621 ggaactaatc cggatactcc aacagtttcc agtgatattc tcattataac acctgatgaa
    10681 gaaaaaatct ggagttttgc cgactgtgcc gtgctgccca atccaactgc cgagcagctg
    10741 gctgacatag ccattgcgac cgcgaatact catcgcagag tcgtaaaaac cgagccgatt
    10801 gttgctatgc tttctttttc tactaaaggc agtgccaaac acgagctagt agataaggtc
    10861 attcaggcta ccgcaattgt gcgaggaaaa tatcccgatt tgagaatcga tggtgagtta
    10921 caagccgatg cggctatcgt acccacaatc ggagctaaaa aagcacctgg tagtcctgtt
    10981 gccggtcagg caaatgtact aatcttcccg gatctcaatg ccggcaatat cgggtataag
    11041 ttagttcaac gcatcggtaa ttgcgaggca atcggtccga tcattcaggg attaaaaaaa
    11101 ccaatgaatg atctttctcg aggatgtagc gttgaagatg tcgttaatgt gacagcaatt
    11161 atctgtaatc tcgtttaaat ataggatcag aaaaatatat gcctacttat gagtataagt
    11221 gtgaaaaatg tggttatgaa tttgaacaat ttcaaaaaat gagtgccgaa ccactaaaag
    11281 tctgccctaa atgtggacaa aaaagtgtca caaggaagat tagtggtggc gccggattaa
    11341 tattcaaagg ctccggtttt tatgtgaatg attatggtga caaaaacaag actaaaagcc
    11401 catctccaac cactacagaa gacaaaccta cttccacacc caaagctaaa gaaaaaacaa
    11461 taacgacatc atctaatagt tccaactgaa tttatattca taaacagctg aaatcagcgc
    11521 ttgattttaa taatttcggt cagctctctg agggcgtttt ttcttctttt ttgcgataaa
    11581 gaattgccag gggaataatt atcaaatagc cgattaaaag tatgataggt gctacggtta
    11641 gcgaacggaa actatatgtt tcaccggtag ccatacaaat ataaccgatt attattactg
    11701 caattccaat tgcaaagagt agataattaa tctttgt
//
LOCUS       DAOMJU010000066        11629 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5622_length_11629_cov_8.349404, whole genome shotgun sequence.
ACCESSION   DAOMJU010000066 DAOMJU010000000
VERSION     DAOMJU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11629
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5622_length_11629_cov_8.349404"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1098)
                     /gene="mtgA"
                     /locus_tag="PLO58_07030"
     CDS             complement(220..1098)
                     /gene="mtgA"
                     /locus_tag="PLO58_07030"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQO74579.1"
                     /translation="MLKLILKIVLFIILIPVVIFVGLYIAAPDGSEFVHKNPETTAFI
                     RQRTAEYQKAGKKVALHWQWVPITKISPNIIHAVILAEDARFYEHIGFDVEAIKFALE
                     KNKKRKKYAFGGSTITQQLAKNLYLSTKKSIPRKVQELIIAFKMERHLSKRRILELYL
                     NVIEFGRGIYGVEAACQHYFGKSAADVSIDEASRLVAIMPSPRRHSPYDGSNFTERRR
                     KWLLNWLYKTGHIDSLDYVILYEGRADNLLEVVDSTGIDQFMQEAIDDSLKSDLEYIS
                     RYLPLDNKIQVSDSIK"
     gene            complement(1217..4060)
                     /locus_tag="PLO58_07035"
     CDS             complement(1217..4060)
                     /locus_tag="PLO58_07035"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HQO74580.1"
                     /translation="MKRFRYPLFFVIFLSLILFNSAQSQVIAEISTDTKTEFGTYHPY
                     KVSVTPSIETYSLPSDYFNVTFNTKYSSYLDQSDIDILQTNQFVVKPTRYKEIFDVYK
                     DCKQNGFPVFVTTDAMLHTFHIIYDYALRALEVQNFAPDLDNLNKALLGTMEALYNNT
                     PDDSLKNIVRKNVAYCGVATKLKDSTAIIPAFVTELVNAELELISAHVGFGKSPIFNS
                     DSVKYKEDYSQYIPRGHYTRNDTLSTYFKALMWYGRMMFRVEPDTTEAGKRKGKEETL
                     QAILIVKAMNELMVNGEPALTVWERIYDPTIFFVGKSDDLDIYEYTNLIKEVYGNDYL
                     SLTVADFADDEKLTEFVEKAKDLQDPLISSSWVWDYEEAEQVTKGFRFMGQRFIPDSY
                     MFQQLVYKKVLLYQGSGEPFTMVMSIIGPIRGFPRGLDVMSVLGSQAAGEIIQAEGDA
                     EYYKYDQQLNKLKDEFANLDEAVWAQNLYWNWLYSLMPLLEPKGEGYPLFMQNPAWTL
                     KQLYAALASWAELRHDTILYAKQSYTSIPTGNHPGPTFTYGYVEPNPPLFARLASLAN
                     LMRTGLESRGLLLSEFESKLINFESLLLSLKKIAEKELTNQELLPEEYNVIWTIGERL
                     EDLLTFSEELMGTSTDETDEGMAVVADVHTEANSSRVLEEGVGYPFEIFVIVKIGNEL
                     VLTRGGGFSYYEFKHPMADRLTDEAWQEMLKSSTPPEIPQWTESFIDLTQSFSLADLQ
                     HAYLEFETVNDIEVRINPVNPISGDTLELNVRAYYCSDSVLTAIFSGDDGISIDSCDL
                     KLIPDDSTRENYTGKIVSSSWPGGTIMVKIKHGIYTLSTHWFELQKGAGVNEDQPMPS
                     RFALSQNFPNPFNPLTTIEYELPQNSHVRLEIYNLLGQKITTLADNHQPAGHYHLIWN
                     GRDSHGNNVASGIYFYKLTAGIFTQVKKMVLTR"
     gene            complement(4101..6563)
                     /locus_tag="PLO58_07040"
     CDS             complement(4101..6563)
                     /locus_tag="PLO58_07040"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HQO74581.1"
                     /translation="MKRFSELFKLALCFSFCFLGLQAQSQMIANISEDVQTDFGIYQP
                     YTANFTPDVPLFSVEPDFSNVENFSDFYGFSAVDSALLLQNHFTVRRSQFKQLYDIYN
                     DCTWDGTPLFVTTDAVLHIYHVLYDKILAEIEIQKFVPALELLTKTLIDSTQSQYDTA
                     TEPEIKETLRRNLAFLCVSQKLLKGSDFTVPEPVSALVDSELTLIANHDGFYTPPVLG
                     PFNLLDYSQFIPRGHYTTNDTLTAYFKAMMWQGWTIFTMEPAKFDNLARRHTLQALLL
                     TQMLFNLDANGNSLLDLWKMIYEPTVFFVGKTDDPNILHYKTIAAQVYGSDFLSLSAD
                     SLANSTLLENFMTEAQKLPEPKIPNWIYGSFTTYKGFRLMGQRFIPDSYMFAHLVLPE
                     VGGRAFPKGLDIMAILGSDRAYDLLDSLYNETAYPNYPEKITEFKTEFAAISPAEWAQ
                     NLYWNWLYCLMPLLYEKGEGYPFFMQTIAWMDKELLTALASWAELRHDTILYAKQSMS
                     PCGIAPGPPKSYVEPNPHLYARLASLTRYTIDGLQARDLLTEKFRDKLALFESLLLFL
                     QDVSIKELENTPLTDEEYANIYCFGKVMQKLVSEGEDPWHYDSDDMAVVADVHTDSDS
                     DQCLEEGVGYPLEINVIVNEGGTIRITRGAIFSYYEFIQPIANRLTDEAWREMLASET
                     PPTMPQWYDGLMDMNASQPSFMENSPDNLYHGEFQAIEKPDDSQKPKTFQVRQNFPNP
                     FNPLTTIEYELPQYSHVRLEIYNLLGQKITTLADNHQPAGHYHLIWNGRDSHGNNVAS
                     GIYFYQLTAGDIIQVKKMVLAR"
     gene            complement(6621..8408)
                     /locus_tag="PLO58_07045"
     CDS             complement(6621..8408)
                     /locus_tag="PLO58_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74582.1"
                     /translation="MQITRIFKWAIGLILVTGIGKATVPDPVTRQPSIPYLMEPIGFR
                     TMQMAIFAPEVSGMVPDKFSDLSWNPAYILREKQKTFYIDFNLENNIESSFSPLSSGI
                     LRRSSYLAPSWYNNTIVQSINIAPLYNLALILPLNKRMSLGIVNRSVFDYGPYRNSIW
                     WTSGGWFDKAIESNSNIRYADFEPQRLETDENQQTVIANQSEITLGYRLSTKTDLGLH
                     LGHYIFHRDGYLLDSQWATHPHSSYGILNDEDLEINGCHYDLGAGFLYHPDEKTTYGI
                     SVGAFFGSGQESEIAQDTSDSFSETATNTAYYCLSEHTLNTDLTYKTSGVKPYLTLIL
                     EKKLNKNLVLRSSFSLTSKNQDVNTRYATDDTTFSDRTYNHWITDTTYAFRRYTANSA
                     AEFNLNGSGEESINQWKFMTALIYSLENDWAFFGGVQISRKWSQVDFDERSNYYSNSF
                     TEYSLYNASTYRNLDTYTKKYSYSQETKAWNLSVPIGVKAYLAKGFSVLLGTDINLTL
                     YKENSYSDLLYPNIVSKYWINDALANDDPETNRYERYDSNPPKTLTRTNAVRFGMVYE
                     HSSGAKLFIRSDGDIMNTNGWTLGFEMNW"
     assembly_gap    8484..8583
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8584..9833)
                     /locus_tag="PLO58_07050"
     CDS             complement(<8584..9833)
                     /locus_tag="PLO58_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HQO74583.1"
                     /translation="MEKTTRNSLILIIFVAGLISCSDVTAPDSVEIPELPRSISKSEQ
                     TLIESSNAFGFDLFKILSNKANGNNLFVSPVSVSMALGMTANGAAGSTLEAMHSTLGF
                     ENLTEDEINQSYQSLINLLRSLDSKVKFRIANSIWYRNEFTALPTFIDVNQTYFDAVV
                     RGLDFNQDAAVDTMNAWIEAQTDGFIKQLIEKPINTNTMMFLLNAIYFKGTWTYQFNE
                     EHTQEAPFYLNNDNTANCQMMSLSSDFDYAETDELQIIDLPYNNGFFAMTVLLPQSSD
                     DLNSLISGMTDEKWNNLIAGLSTTTVSLYLPKFKLEWFQVLNDQLSELGMGIAFDNRA
                     DFSRINDQYGSELYISEVRHKSFIEVDEEGTEAAAAASVEITYKSVSDDTEDITVMRV
                     DHPFIFAIRERASGTILFIGKIMNP"
     gene            complement(9849..11087)
                     /locus_tag="PLO58_07055"
     CDS             complement(9849..11087)
                     /locus_tag="PLO58_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HQO74584.1"
                     /translation="MANYKIMILTLLSLLTLNRCPEKITGGDKPEPIRELSAIEKKVT
                     EADNNFGLKLFRQIISSETDKNIFISPLSISMALGMAYNGADGETRAEMHSMLEFGDL
                     TAAEINQSYRNLIELLSSADPDVTFEIANSIWYRAGFSILSEFLQLNTTYFDAVVQAL
                     DFDRSDATDIINAWVNAKTHEKIPTIINPPIDPLTVMFLINAIYFKGTWTYEFDPEKT
                     QDAPFYLKDGSQTTCEMMRHKATHGYYANDQIQIADLTYGEIGFSMTIILPNAVVDVD
                     SIAAMLTTENWTEWTNSLTEAEINIYLPKFKTEYEIGLIPTLQALGMIQAFSSSEADF
                     DKINPNYDLYISDVKHKTFVDVNEEGTEAAAVTAITVGCTSAGPKEIYFIVNRPFIFV
                     LREKSTSTILFIGKIMNPVS"
     gene            complement(11243..>11629)
                     /locus_tag="PLO58_07060"
     CDS             complement(11243..>11629)
                     /locus_tag="PLO58_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74585.1"
                     /translation="QIGLEHRRQALSGVRLKLITVVSNNDFSEYYGIGLNYYLSGKIG
                     RGYFQVIADLLLKKYRVDLSQYLLYYNPDPEQNIQNQLLVGWELPLWKNLALTGRAAI
                     IRNETHYSGIYYNKWFISGGLIYRKN"
BASE COUNT         3214 a         2400 c         2594 g         3321 t
ORIGIN      
        1 aaaattacca gcaatttttt cattttactc tcctttataa taattttaaa aaaccttgtc
       61 tacccagaca aatgtgcatt aattttaata ttggtatgaa taaaaacaaa gaaaaattat
      121 ttgtgattta ataaaacgaa aaaattgatt aaaaatcata cttcaaaatg tagcaagttt
      181 agaaattaaa aatgtctaat taaaaattga tttttggtat tattttatag aatcactgac
      241 ttggattttg ttatcaagag ggagatatcg cgatatatat tctaaatcag attttaaaga
      301 atcatctata gcttcctgca taaattgatc tattccggtg gaatcaacca cctccagcag
      361 gttgtctgcc ctgccttcgt agaggataac ataatcgagc gagtcgatat gaccggtttt
      421 gtataaccaa ttcaacagcc atttacgtcg tcgttcagta aagttggatc catcataggg
      481 cgaatgacga cgcggactag gcatgattgc aaccaaacgg ctggcttcgt caatgctgac
      541 gtcagccgca gacttgccga aataatgctg gcaggcggct tccacgccat aaattccgcg
      601 tccgaattcg ataacgttca ggtacaactc gagaatacgc cgcttggata gatgacgttc
      661 cattttaaag gcaataatca attcttgaac ttttcgcgga atggactttt tagtcgaaag
      721 gtatagattt ttagctaatt gttgggtaat tgttgaacca ccaaaggcat atttcttacg
      781 tttcttattt ttttcgagtg caaatttgat ggcttccaca tcgaaaccaa tatgttcata
      841 aaaccgagca tcctcagcta aaattactgc atgaataata ttcggtgaga ttttagtgat
      901 aggaacccac tgccaatgga gtgcaacttt tttaccggct ttctgatact cggccgtacg
      961 ctggcgaata aaagcggtgg tttctggatt tttatggaca aattccgaac cgtccggcgc
     1021 cgcaatatat aatccaacaa aaattactac cggaatcaaa attataaata aaacaatctt
     1081 taaaattagc ttaagcataa aatcgaatgg agtttacgga aaatttggta taaggtaaaa
     1141 gagttttgat taatatcgaa aattagtgaa caattctaca atgccaggat tacggggata
     1201 ttttattaga aggtttttac ctcgttaaaa ccatcttttt aacttgcgta aaaatccctg
     1261 cagtcaattt gtagaaataa atcccgctgg cgacgttgtt gccgtgcgag tctcggccgt
     1321 tccagatcag gtgataatga ccggctggct ggtgattgtc cgcgagagtc gtgatttttt
     1381 gtccgagcaa attgtaaatt tcgagcctga cgtgggaatt ctgtggcaat tcatattcaa
     1441 tagtcgttag cggattgaag ggattgggaa aattctgaga aagtgcaaat ctggagggca
     1501 taggttggtc ctcattaact ccagcgcctt tctgtaattc aaaccaatgc gttgataaag
     1561 tgtatatgcc gtgtttgatt ttcaccataa tcgtgccgcc cggccagctc gatgaaacaa
     1621 ttttgccggt ataattttca cgggttgagt catcaggtat caattttaaa tcacaagaat
     1681 caatactgat tccgtcgtcg ccagaaaata tggcagtaag gacggaatca gaacaataat
     1741 aggctctgac attcaattca agcgtatcgc cggagatcgg attgacagga ttgattcgga
     1801 cttcaatgtc attaacggtt tcaaattcaa ggtaagcatg ctgtaaatct gcgagtgaaa
     1861 aactctgagt gagatctatg aaagattcag tccattgtgg aatttccggc ggggtagaac
     1921 tcttgagcat ttcctgccag gcttcgtcgg tcagacgatc cgccatgggg tgtttaaatt
     1981 cataatatga aaatccacct ccgcgcgtta gcaccagctc attcccgatt tttactataa
     2041 cgaaaatctc gaaaggataa ccaactcctt cttctaagac tctggatgaa tttgcctcag
     2101 tatgcacatc ggcaactacc gccatgcctt catcggtttc atcggtactc gttcccatca
     2161 gttcttcgga aaaagtcagg aggtcctcta ggcgctcgcc gatagtccag ataacgttat
     2221 attcttcggg aagtaattcc tgattggtta attccttctc agcaattttc tttaaactta
     2281 gcagaagaga ttcaaaattg ataagcttgg attcgaactc actcagaaga agtccccgac
     2341 tttctaaccc ggtgcgcatt agattagcca gcgaggctaa acgggcaaac aggggcggat
     2401 ttggttcaac ataaccataa gtaaaagttg gaccaggatg atttcccgtc ggaattgagg
     2461 tataactttg tttggcgtaa agaatcgtat catggcgcag ttcagcccaa gatgccaaag
     2521 cggcgtataa ttgcttcaga gtccaggccg gattctgcat aaaaagcgga tagccttcac
     2581 cttttggttc caataatggc atgagtgaat atagccaatt ccagtataga ttttgtgccc
     2641 agaccgcctc atcgagatta gcaaactcgt ctttgagctt atttaattgc tggtcatatt
     2701 tataatattc agcgtcgcct tcagcctgaa taatttctcc ggccgcctga gagcccagta
     2761 cagacatgac gtctaatcct ctcggaaatc cacggattgg tccgataatg ctcataacca
     2821 tagtgaaagg ttctccagag ccctgatata acaggacttt tttataaacc aactgttgga
     2881 acatgtacga atccggaatg aagcgctgtc ccataaaacg aaaacctttg gtaacctgct
     2941 cggcttcttc ataatcccaa acccaggagg aactaattag cggatcctgc aggtctttgg
     3001 ctttttcaac gaattcggtc agtttttcat cgtcagcaaa gtctgccact gtcagggata
     3061 aataatcatt gccgtagact tctttaatca ggtttgtgta ctcgtaaata tccaggtcgt
     3121 cagatttccc cacgaagaaa atcgtcgggt cgtatatccg ttcccagacg gtcagggcag
     3181 gttcgccatt caccattaat tcattcattg ccttgacaat taaaatagcc tggagagttt
     3241 cttctttgcc ttttcgtttt ccggcttcgg tagtatccgg ctcgacccgg aacatcattc
     3301 tcccatacca catcaaggct ttgaaatagg tgctaagggt gtcattgcgg gtataatgtc
     3361 cccgcggaat gtactgactg tagtcttctt tatattttac cgagtcgctg ttgaaaattg
     3421 gagattttcc aaatcccaca tgagcagaaa tcagttccag ctcggcatta accagttcag
     3481 tgacaaatgc cggaatgatc gcggtggaat cctttaattt ggtcgccaca ccgcaatagg
     3541 cgacattttt ccgaacgatg tttttaaggc tgtcatcagg agtattgtta taaagagctt
     3601 ccattgtacc caagagggct ttgttaagat tatccaggtc aggcgcaaaa ttctggactt
     3661 ccaaggctct cagagcgtaa tcgtagatga tatggaaagt gtgcaacatg gcgtcggtcg
     3721 ttacaaaaac cgggaatccg ttttgtttgc aatctttata gacgtcgaat atttccttgt
     3781 aacgggtcgg tttgactacg aactgattgg tttggaggat atcaatgtcg gattggtcta
     3841 aataagagga atatttggta ttaaatgtaa cattgaaata atcggatggc agagagtaag
     3901 tttcaattga aggtgtgacg cttactttat agggatgata ggtcccgaac tccgttttag
     3961 tatcggtgga aatctcggca atcacttgag attgggcaga attaaaaaga atcaaactta
     4021 aaaatatcac aaaaaatagc gggtatctga atcgtttcat attcacattc cttataatga
     4081 tgatttaaac attgaagata ttacctcgcc aacaccattt ttttaacctg gataatatcg
     4141 ccggcagtta attggtagaa ataaatcccg ctggcgacgt tgttgccgtg cgagtctcgg
     4201 ccgttccaga tcaggtgata atgaccggct ggctggtgat tgtccgcgag agtcgtgatt
     4261 ttttgtccga gcaaattgta aatttcgagc ctgacgtggg aatactgtgg caattcatat
     4321 tcaatagtcg ttagcggatt gaaggggttg ggaaaattct gcctgacttg aaaagttttg
     4381 ggtttttgag aatcatcagg tttctcaatg gcctgaaatt cgccatgata gagattatcc
     4441 ggtgaatttt ccataaaaga cggctgactg gcattcatgt ccatcaaacc atcataccat
     4501 tgcggcatag tcggcggcgt ttcactcgct aacatctcac gccaggcttc gtcagtcagt
     4561 cgattagcga ttggctgaat aaactcgtag taggagaaaa tcgccccgcg tgtgatgcga
     4621 atggtcccgc cctcgttcac gatgacattg atttccagcg gatagccgac gccttcttcc
     4681 aggcactgat cggagtcgga atcagtgtgc acgtcggcga caaccgccat gtcatcgctg
     4741 tcatagtgcc agggatcctc accttcggag accaattttt gcatgacttt gccaaaacaa
     4801 taaatattgg cgtattcctc atctgttagt ggtgtattct ccagttcttt gatcgaaaca
     4861 tcctgtaaga aaagcaggag ggattcaaaa agagcaagtt tatcccgaaa tttttcagtt
     4921 aacaagtccc gggcctgcag tccgtctatt gtatagcgcg tgagacttgc cagacgggcg
     4981 taaagatgcg gattgggttc tacataactc ttcggcggac cgggagctat accacatggc
     5041 gacatacttt gtttggcata aaggattgta tcatgcctta gttctgccca ggaagccaga
     5101 gccgtcagga gttctttgtc catccaggcg atagtttgca tgaaaaacgg gtagccttcg
     5161 cctttttcat atagtaatgg catcaggcaa tagagccaat tccaatacag attctgcgcc
     5221 cattcggctg gtgaaatagc cgcaaattcg gtcttgaatt cggtaatttt ttcaggataa
     5281 ttcggatagg cagtttcgtt ataaagcgaa tcgagcagat cgtaagcccg gtccgaaccg
     5341 agaatcgcca tgatgtccaa gcctttgggg aaagctcgac ctccaacttc tggtagtaca
     5401 agatgagcaa acatatatga atccggaatg aaccgctgac ccatcagcct gaaccctttg
     5461 taggttgtaa aagagccgta aatccagttt ggtattttcg gttccggcaa tttttgagct
     5521 tcagtcatga aattttcaag cagagtcgaa ttagccagac tgtcagcgga aagagaaaga
     5581 aaatcagagc cataaacctg tgcggcaatg gttttataat gtaaaatatt gggatcgtcg
     5641 gtttttccta cgaaaaatac ggtcggctcg tagatcattt tccacaaatc caataatgaa
     5701 tttccattcg cgtccagatt aaagagcatt tgagtaagca acagagcctg gagggtgtgt
     5761 ctcctcgcca ggttgtcgaa ttttgccggc tccatggtga aaattgtcca gccctgccac
     5821 atcatagctt tgaagtaagc cgtcagcgtg tcattcgtgg tataatgtcc gcggggaatg
     5881 aattgactat aatccaataa attgaaaggt cccaagacgg gcggagtgta aaatccgtca
     5941 tgattggcga tcaaggtcaa ttccgaatcc acaagcgcgg aaaccggttc tggaactgtg
     6001 aaatcactgc ctttcaataa tttctgtgac acgcacagaa acgccagatt gcgccgtaac
     6061 gtctctttaa tttccggttc ggttgctgta tcatattgcg attgggtgct gtcaatcagg
     6121 gttttggtca aaagttccag agccggtacg aatttttgaa tttcgatttc agccagtatt
     6181 ttatcataaa gaacatgata gatatgcaga accgcgtcgg tagtgacgaa aagcggcgtg
     6241 ccatcccagg tgcagtcgtt gtaaatatca tataattgtt tgaactgact tctgcggact
     6301 gtgaagtggt tttgaagtaa cagggctgag tccaccgcgc tgaatccata gaagtcagaa
     6361 aaattttcaa cattactgaa atcgggttcg acgctaaaaa gcggtacgtc aggggtaaaa
     6421 ttggcagtat atggctggta aatcccaaaa tccgtttgaa catcctcgga aatgttggca
     6481 atcatctggg attgagcctg caatccgagg aaacaaaaag agaaacatag ggcaagtttg
     6541 aataattcgc taaaacgctt catttttcgc ctcgttttta tctgattgaa atttataggc
     6601 aaatttatta ataatcaaca tcaccagttc atctcgaaac ccagcgtcca gccgttggta
     6661 ttcataatat cgccatcgga gcgaatgaaa agctttgcgc cggatgaatg ttcgtaaacc
     6721 atgccaaagc ggacagcatt agtgcgggta agagttttgg gcggattgga gtcatatctc
     6781 tcgtagcgat tggtttccgg atcgtcattg gctaaggcgt catttatcca atattttgaa
     6841 acaatattcg gataaagtag atcgctgtaa ctgttttcct tatacagagt aagattaata
     6901 tccgttccga gtaagactga gaatccttta gcaagatagg ctttgactcc aattggcacg
     6961 cttagattcc aggctttggt ttcctgcgag taggaatatt ttttggtgta agtgtctaga
     7021 ttacggtaag tgctggcgtt gtaaagcgaa tactcggtga aggaattgga gtagtaattc
     7081 gatctttcgt cgaaatctac ctgtgaccat tttcgtgaaa tctgcactcc accgaaaaac
     7141 gcccaatcat tttcgagact gtaaatcagc gcagtcataa atttccattg attgattgac
     7201 tcttcgccgg aaccgttcag attgaattcc gcggcgctat tagccgtata acgtcggaaa
     7261 gcgtaagtag tatcggtaat ccagtgattg taggttcggt cgctaaatgt ggtatcgtcg
     7321 gtggcgtatc gagtgttaac gtcctgattt ttactggtca atgaaaatga tgagcgcagg
     7381 acgagatttt tatttaattt cttttccaaa atcaaggtta ggtatggttt tactccggaa
     7441 gttttatagg tcaaatcggt attcaaagtg tgttcactaa ggcaataata tgcggtatta
     7501 gtagctgttt ccgagaacga gtccgaagta tcctgggcaa tttcagattc ctggccggaa
     7561 ccgaaaaatg ctccgacgct aatcccatag gtcgtttttt catcgggatg gtacaaaaaa
     7621 ccggcgccta aatcataatg gcagccatta atttccagat cttcgtcatt cagaattccg
     7681 tatgaggaat gtggatgagt tgcccattgc gaatccagca agtaaccgtc gcggtgaaaa
     7741 atataatggc caaggtgcaa gccgagatca gttttagtcg aaagccggta gccgagagta
     7801 atttcggatt ggttggcaat aaccgtctgc tggttttcgt cagtttcgag acgttgcggt
     7861 tcaaaatcgg cataccgaat gttggaattt gattcaatag ccttatcgaa ccagccgccg
     7921 ctcgtccacc agatcgagtt tcgatagggg ccatagtcga agactgaacg gttgacaata
     7981 cccaggctca tgcgtttatt caatggcaga atcaaagcta gattgtaaag cggcgcgata
     8041 ttgatactct gaacgatggt gttattatac caggaaggtg cgaggtaaga actccgcctc
     8101 aggatgcctg acgatagggg actaaaagaa gattcgatat tgttttcgag attgaaatca
     8161 atataaaatg tcttttgttt ttcgcgcaaa atataggctg gattccagct cagatcgctg
     8221 aacttgtccg gaaccatgcc actgacttcc ggcgcgaaaa tcgccatctg catcgtgcgg
     8281 aatccaatcg gttccattag ataaggaatc gacggctggc gcgtgaccgg gtcgggaact
     8341 gttgcttttc ctattccagt cactaaaatt agacctattg cccatttaaa aattcgagtg
     8401 atttgcatat ttgctccttc atatatttgg attcatgatt ttcccgatga acaagattgt
     8461 gccggaagca cgctcgcgga tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnggattca tgattttccc gatgaacaag attgtgccgg aagcacgctc gcggatcgca
     8641 aaaataaaag gatgatcaac gcgcattacc gtaatgtctt ctgtgtcatc cgagaccgat
     8701 ttgtatgtaa tctcgacgga tgccgccgcc gcggcctcgg tgccttcttc atcaacttca
     8761 ataaaagatt tatgtctgac ttcgctgata tataattcag agccatattg gtcgttgata
     8821 cggctgaaat cagcccgatt atcaaatgcg attcccatac caagttctga tagctgatca
     8881 tttagcacct ggaaccattc gagtttgaat ttcggcaaat acagactgac agttgtcgtg
     8941 gataagcctg cgatcagatt attccatttt tcatcggtca tgccacttat caacgagttt
     9001 aaatcatcac tggattgcgg caggagtacc gtcatggcaa aaaatccatt gttataaggc
     9061 aggtcaataa tctgcagttc gtcggtctcg gcataatcga aatcactact gagtgacatc
     9121 atctggcagt tcgccgtgtt gtcattattt agataaaacg gagcctcctg ggtgtgttct
     9181 tcattgaatt gatatgtcca ggttccctta aaatagatgg cattcagcag aaacatcatc
     9241 gtattggtgt tgattggttt ctcaatgagc tgcttgatga atccatcggt ttgagcttcg
     9301 atccaggcat tcatggtatc aacggcggca tcctgattga aatcgaggcc ccgaacgacc
     9361 gcgtcgaaat acgtctggtt aacgtcaata aatgtgggta aagcagtgaa ttcgttgcga
     9421 taccaaatcg agtttgcaat cctgaatttg actttcgaat cgagcgatct cagtaaattg
     9481 atcagacttt gatacgattg gttgatttca tcttcggtca aattttcgaa gccgagtgtc
     9541 gaatgcatag cctccagagt agaacctgcc gcgccattcg cggtcattcc taaagccatc
     9601 gaaaccgaaa ccggtgacac gaacaggttg ttgccattgg ctttatttga tagaatttta
     9661 aaaaggtcaa agccaaaggc attgctcgat tcaattaacg tctgttcgga tttactgatg
     9721 cttctcggta actctggaat ttctacggaa tccggagccg tgacgtccga acagcttatt
     9781 aacccagcca caaaaattat aagtattaaa gaattacgag tggttttttc catctttaat
     9841 tctccttatt atgaaaccgg attcatgatt ttcccgatga ataggatcgt gctggtgctt
     9901 ttctcgcgca aaacgaaaat gaacggacga ttaacgataa aataaatctc ttttggaccg
     9961 gctgaagtac atccgaccgt tatggcggtc actgccgcgg cttcggtgcc ctcttcgtta
    10021 acatcgacaa aggttttgtg tttaacatcg ctaatataca gatcgtaatt tgggttgatc
    10081 ttgtcaaaat cagcctcaga agaactgaaa gcctgaatca ttcccagagc ttgcaaagtt
    10141 ggaatcaaac caatctcgta ttcagtcttg aacttgggta gataaatatt gatttcagct
    10201 tcggtcagac tattagtcca ttctgtccag ttttccgtgg ttagcattgc cgcaatcgaa
    10261 tcgacatcta ctacggcatt tggcaggata atagtcatac tgaagccaat ttcaccgtaa
    10321 gtcaggtcgg cgatctgaat ctggtcgttg gcatagtaac catgggtggc tttgtggcgc
    10381 atcatctcac aggtcgtctg actgccgtct tttaggtaga atggcgcgtc ctgtgttttt
    10441 tccggatcga attcataagt ccaggtgcct ttgaaataaa tcgcgttaat caggaacatc
    10501 actgtcaatg gatcaatcgg cggattaatg atagtcggga ttttttcatg agtcttggca
    10561 tttacccagg cgttgattat atcagtcgca tcactgcggt caaagtccag cgcctgaacg
    10621 accgcgtcga aataggtggt atttaactgc aaaaattcgc tcagaatcga aaaacctgct
    10681 cgataccaga tggagttggc gatttcgaat gttacatcag gatcagcact cgagagaagt
    10741 tctatcagat tgcgatagga ctgattgatc tctgcggctg tcaggtcgcc gaactccagc
    10801 atggaatgca tttcagcgcg ggtttcaccg tccgcaccgt tataagccat gccgagcgcc
    10861 atcgaaatgc tcaggggtga aatgaagata tttttatcgg tttcggagga aataatttgc
    10921 cggaataact tcagaccgaa gttgttgtcg gcttctgtca ctttcttttc aatggcgctc
    10981 aattcacgga tcggttctgg tttgtcaccg ccggtaattt tctcaggaca gcggttcaga
    11041 gtaagcaggg aaagcagtgt cagaatcata attttgtagt ttgccataga agcctcatca
    11101 tttattttcg caatacttaa aaacgacaat ttatttatga caaatagcat tccaagacct
    11161 tccgacggag aaaatctgtc cggtatagac aaaccctcag ctttctgaga ctaacataga
    11221 aaatctgata tttttgagac gcttagtttt tacggtagat caagccgccg ctaataaacc
    11281 atttattgta atatatgccg ctgtaatgag tttcattacg tattattgcg gctcgaccag
    11341 tgagggctaa atttttccag agcggcaatt cccacccgac gagcagttga ttctgtatgt
    11401 tctgctccgg atcgggattg taataaagga ggtattggct tagatcaacc cgatatttct
    11461 tcagtaataa atctgcgatg acctgaaaat agccgcgccc aatttttccg gataggtaat
    11521 aattcagacc gataccgtaa tattccgaga aatcattatt ggagacaaca gtgattaatt
    11581 tcaggcgcac accgctgaga gcctggcgac gatgttccag tccgatctg
//
LOCUS       DAOMJU010000067        11542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5697_length_11542_cov_15.506137, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000067 DAOMJU010000000
VERSION     DAOMJU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5697_length_11542_cov_15.506137"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2669)
                     /locus_tag="PLO58_07065"
     CDS             complement(<1..2669)
                     /locus_tag="PLO58_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQO74586.1"
                     /translation="MFFSAQVLIAQVVYSNPPHPTERDSIIIYFDATKGDGGLKDFTG
                     DVYAHTGVITNLSATAKDWRYVITPWPGQGGTANTSKNKLERISTNLYKLTIGFPRTF
                     YISHENGATIPDGEKILKLAFVFRNADGSKSGRDVGGADIFQNLFEVGLTVIVSEPVI
                     DLSLSDPRRSPVFTNYGDTVNIKITTAALNTKVSVIKLLVNHELINQVADTAMSFDFV
                     AEEYNYGMNNLMAIGTDTSGFSDTASFAIMINPPLTSISRPEGIVDGVNVLNNNSITF
                     SLQAPFKDFVYLIGDFNDWLVDTSYFMHCEDNGGTDVHWWLTIDDLDSQTEYAFQYFV
                     DGEIRLADPYSEKILDPWNDQYISASVYPNLKPYPAGKTAEIVGTFQIDQPKYSWQTA
                     DYTRPEKTNLIIYEMLIRDFIDAHDFKTLIDTLDYLQRLGINAIELMPVSEFEGNSSW
                     GYNPSFYFAVDKYYGPADDFKHFVDECHRRGIAVIQDIVLNHSYDSSPLVRLYWDKVN
                     SRPSSENPWYNQVSPNPVYSWGYDFNHESQDTKNFIDRVTSYWLTDFRVDGFRLDFTK
                     GFTNKPGEGFAYDASRIAILKRIADKIWEIDTSAYVILEHFADNSEEKILADYGMMLW
                     GNSNWNYAQSSMGYTNESDFSWGFYKSRSWNQPHLVTYMESHDEERLMYKNLTWGNSS
                     GSYNIKTLNIALERIKMTAAFFLTLPGPKMLWQFGELGYDYSIEYNGRLGEKPIRWDY
                     YQDPDRLRLYKTIQALIKLRSENDVFTSASTTPTMTLSGNVKSIKMSGSNLNVVIIGN
                     FGVVNAETAVSFQHGGVWYDYFSGESFNFSGFDSVNLAPGEFHIYTDQKLATPEEGLV
                     AIAEADANNLPKQFQVYPNYPNPFNSLTTFEYD"
     gene            complement(2716..4527)
                     /locus_tag="PLO58_07070"
     CDS             complement(2716..4527)
                     /locus_tag="PLO58_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQO74587.1"
                     /translation="MPRVLPLIFALLMILNNCEINQNLQSLNGKWLFKYDRNGIGDTQ
                     GYYRTEYDRKDWSTINVPAFWDDPHYDGIGWYATEFKLKKPLGNNRLALVFEAVDDDA
                     VVYLNGEKVGEHTGYGIQFYLDITHQIKPETSNLLVVRINDTGGAGGITGNVLLRYYR
                     DETELLQSEFSQEEANPVPDWVKSACLYEVFVRAYSPEGDFQALTRDLQRLSLLGVDC
                     IWLMPIFPIGAKHRKGSMGSPYSIRDFKSINPDLGTESDLKNLITSAHQLGIKVILDI
                     ACNHSSWDNLLIEEHPNWYSQDAQGKIIAPNENWSDVADFNYNQTGLREYMWEVLEYW
                     VKEFDIDGYRLDVAELVPDDFWKIAAHRLQKIKPDILMLAEGDHPRLYLNGFHLTYGW
                     NTRRAFYQIIKNDYPAEFLAQTLRKEYYRYPQHALKMRFTENHDEARTMALFSPEQTM
                     VLTFLIFTLPGVPMIYAGQEIGVSEKPSLFEKDTINWHQFDKSLYELITKMNELRRNI
                     STFSSGSYQVINTSDPESIFAFSRYNAQEVVLIVTNLKSKSQMATIDLAPISAKIPNT
                     EPELLVGTGAFLLENNTLYLPCYGYDYYLFRFETKND"
     gene            complement(4560..5393)
                     /locus_tag="PLO58_07075"
     CDS             complement(4560..5393)
                     /locus_tag="PLO58_07075"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQO74588.1"
                     /translation="MDIFQDNVLHKMMQLGFTNYEARTYLGLLKLSPATGYEISQLAH
                     VPRSVVYSVLKQLESRGIVISIHDKPRRYIPLAPDEMLKRMETDYASRVSDLKDDLAE
                     LSNKSGTEGFWNLRGYQSLMQTCETLIREAHKSIYISGWQREVSLLREHLLNACKRNV
                     FVVIFSFNEINPPIGVTLAYGIPEEKLEHYWDRKLILVTDSKNLIMGPANQKEDEQAI
                     WTQNKAALTIATNYIILDITLFGQRMNMDISNIIAGMLTDEFEKVDDLIQEAIEKHKK
                     A"
     gene            complement(5414..7246)
                     /locus_tag="PLO58_07080"
     CDS             complement(5414..7246)
                     /locus_tag="PLO58_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQO74589.1"
                     /translation="MHIVDYFIVCIYLIGILIVGLWFQRKASEGINSYFLGNRSLPWW
                     ALGASGMASNLDISGTMIIVALVYALGARGLYIEFRGGVTLIMAFLMIFMGKWNRRSK
                     VMTLAEWMEFRFGKGTEGRLARMITALTSILVTIAMVTYFAVGGGKFLDKFLGIPNFL
                     GLSSQFWAAAVLIAIAMIYTVASGLYGVVWTDVFQGSLIFLMILYVVIQVFHFDIPSE
                     FSVVMPLKDAAGGGFQFFPTTYQEWTDVLPKFKLSIDSSSVYSMYNLFGIAVLFYLLK
                     VVIEGCGGTGNYMIQRYYAAKDDREAGLLSLFWTFLLSFRWLFVGAMAVWGIYLGTQI
                     TDPEMVLPIVVQTLPIGIKGFLVAGMMAAAMSTFDSTVNAGAAYWVKDIYLEYINKNA
                     SDHQQMRQSYGASIIIVVLGLVLMLIIKNINEIWGWITMSIGAGLIVPQLLRWYWWRL
                     NGYGYTIGMVAGMLASIIWKIIAPEATAEYWSFLFAAGISTVATVIGTLLTEPTDEKV
                     LDNFYRVTRPFGFWGPIRAKLPEKLISQINRENRRDIISIFFAVPWQVCLFLMWMVLV
                     MKRYDTFLSLLLIVVTLSTILYFTWFRHLATEVNIESDADSSSG"
     gene            complement(7321..9372)
                     /locus_tag="PLO58_07085"
     CDS             complement(7321..9372)
                     /locus_tag="PLO58_07085"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HQO74590.1"
                     /translation="MKKATVLMLMLTLIAVSGLADVTVNYIVNTSTLQGVTDSTHQVQ
                     ICGAEVGPEGVDKWYNLFLTWGDDSPLAANIGGDYWKLTIAYPDSMIGWRMAYKVRYK
                     ANDDDAFTWENVTGGGNRMWVLPANDTTLAVAYVNNDFNPPFTPSDSVDILVRVNMEN
                     YEDSFSLLSVVGHWPFCDPAWTPGALPLTQEGTSDYWSRTIRIPQAVMDTVDRVQPLG
                     AEHPGVLMYRFAIGNDWNNSENLNGKYWPGNENRIIALHPGLEDTTLAWKYWNDNPPA
                     GFIAEDTVDITFTADLTNAIANKGFAVGDTILVRMGYFNSLSSTMDANLLRISGTYLY
                     STTFTDVPVVLNEPLYYQYYLVKDAVEQREVYFNFDYEGEDVTRAERRAYVITGDAEI
                     NDIEDSETDPRRMPKFRNNQKLSQAVTVTIECDLRPAYYQVWAGSTLDDIQGNIDITP
                     AMLEANPDTLAKLGLCINGPMSNNGEGTWATWGGTLASDATRRMYDDGTNGDETAGDT
                     IYTIVYNFTTEKTIGQEFKFGIGGGDNESSYGLNHIENIDDTNPTFTLEVQFGSINPN
                     FYSAWDFNARAPKVGIKNQNVSVAKEFSLSQNFPNPFNPTTEIRFTISEKSDVHLTIY
                     NLLGKVVNKVTYNNLDIGNYSYVWNSKDLNGNSVASGIYFYELRAGSKYHAIKKMVLM
                     K"
     gene            complement(9395..10408)
                     /locus_tag="PLO58_07090"
     CDS             complement(9395..10408)
                     /locus_tag="PLO58_07090"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO74591.1"
                     /translation="MNRKLPTIISTVLISTIFLNAQNKEGTAAGQFLQIPVGGKAIAM
                     GGAFTALADDASAIYYNPGAISRISKNKLYVSQTNWLVGGKHQFIAAQVMATSNDIIG
                     IGVDYLDYGEREKITTVAEPDGTGEYWEASDLAFSLGYARNLTDRFSIGGSVKFIQQN
                     IWHVSATGFAADLGLLFKTQFNGLQIGASMRNFGSELQLTGRDLITQVDLDEEASGNN
                     ETIVAYLKTDKWPIPLTFTIGVAMPVINSNQFVLTLAGDVVRPTDNSQTLNCGAEAII
                     MKMISLRAGYQALFREKTQQGLTAGIGLKLPIQYADFYFDYSYQEFGFFGDLSTMSIS
                     IGF"
     gene            complement(10419..>11542)
                     /locus_tag="PLO58_07095"
     CDS             complement(10419..>11542)
                     /locus_tag="PLO58_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74592.1"
                     /translation="DDILIASESDPNNYLSLNKFEIDTLRGKIKIKAGSNLKSGSYRI
                     FYKYYPVFKSPNIQYSPYMKETKDSDIFDGVELVFSNPWDIKMIKSKSGWNSTDRIID
                     FTFRIDQALLEGDTIVGVQYPGIYELRVGDSSEVLYETPKEFLKSIYPSFSDAIIRTL
                     VKKTNFYLFNVLDSVKIPFLYNGGIKNSYGIYEITPGGLIESYIPKPDSTYQYSWVIN
                     FPPVSEKVTPPRFLKGDILHIETEIPFRNGDIYEFTTEIPNVDDEVAKSSLDDIQVVP
                     NPYVVANTMESPLPPAITSGRGERRIEFRKLPNDAKVHIFTSAGAHVITLNHNGNIHN
                     GTLAWNVKSKENLDVAFGVYYYIIESSVGKKSGKLAVIK"
BASE COUNT         3450 a         2542 c         2208 g         3342 t
ORIGIN      
        1 aaatcatatt caaaagtcgt taatgaattg aatggattgg gatagttcgg atagacttgg
       61 aactgtttag gtaaattgtt tgcgtcggct tcagctatag caactaatcc ttcttcgggt
      121 gtcgccagct tttgatcggt ataaatgtga aattctcccg gcgccaggtt tactgaatca
      181 aatccgctaa aattgaagct ttcccctgag aaataatcgt accagacgcc gccatgttga
      241 aaagaaactg cagtttcagc attaacaact ccaaagttgc cgatgataac tacattcaga
      301 ttactgccgc tcattttaat gctcttgaca ttgccgctta gtgtcatagt tggcgtggtg
      361 ctggcgctgg taaagacgtc attttccgac cttagtttta tcaaagcctg aatagtttta
      421 tacaatcgta agcgatccgg atcctgataa taatcccagc gaatcggttt ctcccccaga
      481 cgtccattat attcgatgga ataatcatat cccagttcgc cgaattgcca gagcattttc
      541 ggaccgggca aagtcaggaa aaaggcggca gtcattttaa tccgctccag cgctatgttc
      601 aaagttttaa tattgtaaga gccggaagaa ttgccccagg ttaggttttt gtacatcagg
      661 cgttcttcgt catgactttc catataagta actagatgcg gttggttcca agaacgactt
      721 ttataaaaac cccacgagaa atcagattca ttagtataac ccattgatga ttgggcataa
      781 ttccaatttg aattgcccca gagcatcatt ccataatcag ccagaatttt ctcctcacta
      841 ttatcagcga aatgttccag aataacataa gcgctggtat caatttccca gattttatcg
      901 gcgatacgtt tcaaaatggc aatccggctg gcgtcataag caaaaccttc gccgggttta
      961 ttggtgaaac ctttcgtaaa atccagacga aagccgtcaa ctctgaaatc cgtcagccag
     1021 taggacgtaa ctcggtcaat aaaattctta gtatcttgac tctcatggtt aaaatcataa
     1081 ccccaggagt aaacgggatt cggcgagacc tgattatacc aaggattttc tgacgatggt
     1141 cggctattta ccttgtccca ataaagccgt accagcggcg acgagtcgta ggaatgattc
     1201 agtacaatgt cctgaataac ggctatgccg cgccgatgac attcatctac aaagtgttta
     1261 aagtcatctg ccggtccgta atacttatcc acagcgaaat aaaatgacgg gttgtagccc
     1321 caggatgaat tgccttcaaa ttcactaacc ggcattaatt ctatggcgtt aatacccaat
     1381 cgctgtaaat aatccaacgt atcaatcaaa gttttaaaat catgcgcgtc aataaaatcc
     1441 cggattagca tctcataaat gatcaggttg gttttttccg gacgagtgta atctgcggtt
     1501 tgccaactat atttgggttg gtcaatttga aaagtgccga cgatttccgc cgtttttcca
     1561 gctggataag gtttcagatt gggatagacg gatgctgaaa tatattgatc attccatgga
     1621 tccagaattt tttcggaata aggatccgcc aggcggattt caccatcaac aaagtattgg
     1681 aaagcatatt cggtctgtga atcgagatcg tcaatggtca gccaccagtg cacgtcggta
     1741 ccgccgttat cttcgcaatg cataaaatag ctcgtgtcaa ccagccaatc attaaagtcg
     1801 ccaatcagat agacgaaatc cttaaaaggc gcttggagtg aaaaggtaat gctgttgtta
     1861 tttagcacat tgacaccgtc aacaatacct tcgggtcgag aaatagacgt cagcggtgga
     1921 tttatcataa tcgcaaaact ggcggtatcg ctgaatccac tcgtgtcagt accaatcgcc
     1981 attagattat tcattccata attatattcc tctgccacaa agtcaaaact catcgcagtg
     2041 tcagcgacct gattgattaa ctcatgatta actaatagtt ttatgacact aaccttagta
     2101 ttgagcgcgg ctgtggtaat ttttatgttt accgtatcgc cataattcgt aaaaacaggt
     2161 gagcgacgcg ggtcggatag gcttaaatca atgacgggtt cactgacaat taccgtcaat
     2221 cctacttcaa ataagttttg gaaaatgtcg gctccaccaa catccctccc actcttactg
     2281 ccatcagcat tgcggaagac gaaggctaat ttgagaattt tttcgccatc gggaatagtt
     2341 gcgccatttt catgagaaat gtaaaaagta cgcgggaagc cgatcgtcag tttgtacaag
     2401 ttggttgaaa ttctctcgag tttatttttc gaagtattag ctgtaccacc ttgcccgggc
     2461 caaggagtta tgacatagcg ccagtcttta gcggttgcgc ttaaattggt aataacgccg
     2521 gtatgcgcat aaacatcgcc cgtgaaatct ttcaaaccgc cgtcgccttt agtggcatcg
     2581 aagtaaatta tgatcgaatc ccgttcggtc ggatgaggcg ggttagagta aaccacctga
     2641 gcaatcagaa cttgagcact aaagaacatc aagcatagta cgcctatttt gtaaaaaatt
     2701 ctgttaaggt ggttattaat catttttggt ctcaaaccgg aataaataat aatcatatcc
     2761 gtaacacggc aaatataatg tattattttc cagcaaaaat gctccagttc cgaccaataa
     2821 ttccggttca gtattgggaa ttttcgctga aatcggcgcc aaatcaatcg tcgccatctg
     2881 agatttactt ttcaaattgg taacaataag tacaacttct tgagcattat atcgagaaaa
     2941 tgcgaagatg ctctccgggt cggaagtatt tatcacctgg taggagcccg atgagaaagt
     3001 agaaatgttg cgccgcagtt cattcatttt cgtgattaat tcatataaac ttttgtcaaa
     3061 ctgatgccag ttgatagtat ctttctcgaa tagagatggc ttttcgctaa cgccaatttc
     3121 ctgtccagca taaatcatcg gcacacccgg caaggtaaat atcaggaagg ttaagaccat
     3181 agtctgttca ggtgaaaata aagccatagt tcgcgcttca tcatgatttt cggtaaaacg
     3241 cattttgagc gcatgttgag gataccgata atattctttt cttagagttt gggctaaaaa
     3301 ttcggctgga taatcattct taataatctg ataaaaagcc cgccttgtat tccaaccata
     3361 ggtcaaatga aaaccattaa gatacaaacg aggatgatcg ccttccgcca gcattaaaat
     3421 atccggtttg attttttgga ggcggtgcgc ggctattttc cagaaatcat ccggtaccag
     3481 ttcagcgaca tcgagccggt atccatctat atcaaactct ttgacccaat attctaatac
     3541 ctcccacata tattcacgca aaccggtttg attataatta aaatcggcta cgtcactcca
     3601 attctcattc ggggcaatta ttttaccttg tgcatcttga gagtaccaat ttgggtgttc
     3661 ttctataagt agattatccc aggagctgtg attgcaagca atatcgagaa taacctttat
     3721 tcccagttga tgagcgctgg tgatgagatt tttcaaatcc gattctgttc ctaaatccgg
     3781 attgatactc ttgaagtcac ggattgaata gggactgccc attgagcctt tccggtgttt
     3841 agcgccgatt ggaaaaatcg gcatcaacca gatacaatct actccgagca aactcagacg
     3901 ttgtaaatct ctggttagtg cctgaaagtc gccttccgga ctataggctc tgacgaatac
     3961 ttcataaagg caagctgatt ttacccaatc aggaactgga tttgcttctt cttgagaaaa
     4021 ttctgattgt agaagttctg tctcatccct atagtaccgc aaaagaacat taccggtaat
     4081 accgcctgct ccaccggtat cattgattcg cacgactaat aaattggatg tttccggttt
     4141 gatttgatga gttatatcaa ggtaaaattg gattccgtat ccggtatgtt cgccaacttt
     4201 ttcgccgttt agatatacaa cggcgtcatc gtcaaccgcc tcaaaaacaa gtgccagtcg
     4261 gttattaccc agcggttttt taagtttaaa ttcagtcgcg taccaaccga ttccgtcata
     4321 atgcggatca tcccagaagg ctggaacatt tatagtgctc cagtctttcc gatcatattc
     4381 ggtgcggtaa taaccttgag tatcaccgat gccgttacga tcgtacttga acaaccattt
     4441 gccgttcaaa ctctgcaaat tctggtttat ttcacaatta tttaaaatca taagtaatgc
     4501 aaaaattaaa ggtaacactc gaggcatatt ggtcattcca ggttaaatcg gttggagtct
     4561 taagcctttt tgtgtttttc aatggcttct tgaatgagat catcgacttt ttcaaattcg
     4621 tcagtcaaca tacccgcaat aatgttagaa atatccatat tcattcgctg accgaaaagg
     4681 gttatatcca gaataatata attggtagca attgtcagag ccgctttatt ctgagtccag
     4741 atggcttgct catcttcttt ctgattagct ggtcccataa ttaaattttt cgaatcggtt
     4801 accaaaatta attttcgatc ccaataatgt tccagttttt cttctggaat gccataagcc
     4861 aaggttactc cgattggcgg attaatttca ttaaaggaaa aaattacgac aaaaacattg
     4921 cgtttgcagg catttagtaa gtgctctcta agaagcgaaa cctcacgttg ccagccggaa
     4981 atgtagatag atttatgagc ttcgcggatt aacgtctcac aggtttgcat taaagattgg
     5041 tacccacgta agttccagaa tccctcggtg cctgatttat tactgagttc ggctaagtcg
     5101 tctttaaggt ccgatactcg actggcataa tcggtctcca tccgcttcaa catctcgtcc
     5161 ggtgccaaag ggatatatcg gcgcggttta tcatgaatcg aaattacaat accacgcgat
     5221 tctaactgct ttaataccga gtaaactacg gatcgaggaa cgtgagccaa ttgactgatt
     5281 tcatagccgg tagcaggact caatttcagt agtccgaggt aagtgcgggc ttcatagtta
     5341 gtaaagccca gttgcatcat tttatgaaga acattatcct gaaaaatatc catttttttc
     5401 tttctatttt aggttacccc gaagatgaat cggcatcgga ttcgatgttt acttctgtcg
     5461 caagatgacg aaaccaggta aagtataata ttgttgatag ggttactaca atcagtaata
     5521 agctaaggaa agtatcatag cgtttcatca ctagaaccat ccacatcaaa aacaggcaca
     5581 cctgccaagg cacggcaaag aaaattgaga ttatatcgcg tcgattttca cgattgattt
     5641 gagatatcag tttttcgggt aatttagctc ggattggtcc ccaaaaacca aatggacggg
     5701 taactcggta gaaattatct aatacttttt cgtcagttgg ttcggttaaa agggtgccga
     5761 taaccgtagc gacggtacta attccggcgg caaatagaaa tgaccagtat tcggcggttg
     5821 cttcaggggc gataattttc cagatgatcg aagctagcat tccagcaacc ataccgatag
     5881 tataaccata accatttagt cgccaccaat accagcgcag taattggggc acaatcaagc
     5941 cggctcctat actcatagta atccagcccc aaatttcatt aatattctta ataataagca
     6001 ttagaaccag tccaagaact acaataatta tcgaggctcc atagctttgc cgcatttgct
     6061 gatgatcaga agcattttta tttatatatt caagataaat atctttgacc caataagccg
     6121 caccagcgtt aacagtcgag tcgaaagttg acattgccgc cgccatcata ccagccacca
     6181 gaaatccttt tattccaatt ggtaaagttt ggaccacaat gggtaaaacc atctcgggat
     6241 cggtaatttg agttccgaga tatattcccc agactgccat agctcccaca aaaagccagc
     6301 ggaatgataa taaaaatgtc caaaataacg agagtaatcc agcttcacga tcatctttag
     6361 cggcgtagta acgttgaatc atatagttgc cggtccctcc acaaccctcg ataactacct
     6421 taagcaaata aaacagtaca gcgattccga ataaattgta catactatag acactgctgg
     6481 aatcgatcga taatttgaat ttggggagaa cgtcggtcca ttcctgataa gttgtgggga
     6541 aaaactggaa tccacctcct gcagcatcct tcaaaggcat aactacagaa aattcagacg
     6601 gtatatcaaa atgaaacacc tggataacta cgtataaaat cattaaaaat atcagtgaac
     6661 cttgaaatac gtctgtccac accactccat ataatccgga agcaaccgta taaatcatcg
     6721 caatggcgat cagaaccgcc gccgcccaaa attgtgacga taaacccaaa aaatttggaa
     6781 tacctaaaaa tttgtctaaa aattttcctc cacccacagc aaaataagtt accatcgcaa
     6841 ttgtgaccag aatcgaggtc aatgccgtaa tcatccgcgc taagcgcccc tctgtacctt
     6901 tcccaaaacg gaattccatc cattccgcta aagtcattac ttttgaacgc cggttccatt
     6961 tacccataaa tatcattaaa aatgccataa tcaaggtaac acccccccga aattcgatat
     7021 ataaacctcg ggctcctaag gcataaacca gggcaacaat aatcatagtt ccgcttatat
     7081 caagattgga tgccatgccg gatgctccta aagcccacca aggcaatgac cgattaccca
     7141 gaaaatatga attaataccc tcagaggctt tacgctggaa ccataaaccc actatgagga
     7201 ttcctatcag ataaatacag acaataaaat aatctactat atgcatctgg cgatctcctt
     7261 aatgaacaat caaagaaaaa gaggaaggac ggaaaatccg tccttcctct taagagcaac
     7321 ttatttcatc aaaaccatct ttttgatagc atgatattta ctaccagccc gtagttcata
     7381 gaaataaata ccggaggcaa cactgttgcc gtttaggtct ttactattcc agacgtagct
     7441 gtaattgccg atgtccaggt tattataagt aaccttattg acaaccttac cgagcaggtt
     7501 gtatatcgtc aaatgaacat cgcttttttc cgagatagta aaacgaattt ctgtagttgg
     7561 attgaatgga tttgggaaat tctgagacaa agaaaactcc ttagcaacac taacattttg
     7621 attcttaata ccaacttttg gtgctctggc attaaaatcc caagccgaat agaaattcgg
     7681 attgatcgag ccaaactgaa cctccagtgt aaaagtcgga ttcgtgtcat caatattttc
     7741 tatatggtta agaccataac tgctttcatt atctccgccg ccaatcccga atttgaattc
     7801 ctgaccgatg gttttttcag tggtaaaatt gtatacaatc gtataaatgg tatcaccagc
     7861 agtctcgtca ccattagttc catcgtcata cattctgcga gtagcgtcac tggctaacgt
     7921 tccaccccaa gttgcccagg taccttcgcc attgttggac attgggccat tgatacataa
     7981 acctaattta gcgagagtat ctggattggc ttccagcatc gctggggtaa tgtcaatgtt
     8041 gccttgaata tcgtcaagcg tcgaaccagc ccagacttga taataggccg gacgcaggtc
     8101 gcattcaatt gttaccgtaa cagcttgact aagtttctgg ttattcctga attttggcat
     8161 gcgacgtgga tcggtttcgc tatcctcgat gtcgttgatt tcagcatcgc cggttatgac
     8221 gtatgcacgt ctttcagctc gcgtaacgtc ttcgccttca taatcaaaat tgaaatatac
     8281 ttcacgttgt tctacggcat cttttacaag ataatactgg taataaagcg gttcgttaag
     8341 aactactgga acatctgtaa aagtggtcga atataggtag gttccggaaa ttcgaagcag
     8401 attagcatcc attgttgaag acaaactgtt aaaataaccc atccggacta aaatggtatc
     8461 gccaactgca aaacctttat tggcaatggc attagttaaa tcggcggtaa aagtaatatc
     8521 aacggtatct tccgctataa atccggccgg tggattgtca ttccaatatt tccaagccaa
     8581 ggtcgtatct tccaagccag gatgcagagc aatgatccgg ttttcattac ctggccagta
     8641 tttaccattc aagttttctg aattgttcca atcattaccg atcgcaaaac gatacatcaa
     8701 cactccggga tgctctgcac caagaggttg tactcgatca acggtatcca tcacagcctg
     8761 tggaatccgg atcgttctgc tccagtaatc ggaggtgcct tcttgagtca aaggcagtgc
     8821 cccaggtgtc caggctggat cacagaaagg ccaatggcca acaaccgaca atagtgagaa
     8881 actgtcttcg taattctcca tatttactcg aactaaaata tcaacggaat cagacggtgt
     8941 aaatggcgga ttgaaatcat tgttaacata cgccaccgcc agggttgtgt cattagctgg
     9001 taaaacccac atacgattac ctccaccagt gacattttcc caggtaaagg catcatcatc
     9061 attagcctta taacgaacct tataagccat ccgccagccg atcatcgaat ccggataggc
     9121 aatcgtcagc ttccagtaat ctcctccaat atttgcggct agtggtgaat catcgcccca
     9181 ggtcaaaaag aggttgtacc acttatcaac accttctggc ccgacttccg caccacaaat
     9241 ctggacctga tgggtcgaat cggtgacccc ttgcagagtt gatgtattga caatataatt
     9301 aactgtcaca tcggctaatc ccgagacagc aattagagtc aacatcaaca tcaaaacagt
     9361 agcttttttc atagcttact cctttctttt attgttaaaa accaattgat atagacattg
     9421 ttgaaagatc cccaaaaaat ccaaattctt gataagaata atcaaaataa aaatcggcat
     9481 attggattgg caatttcaag ccaataccag cggtcaatcc ttgctgtgtc ttttcgcgaa
     9541 ataaagcttg ataaccggca cggagactaa tcattttcat tattattgct tcagctccac
     9601 agttaagtgt ctggctgtta tccgtaggac gtacgacatc acctgccagt gtcaaaacaa
     9661 attgattact attgatcacc ggcatcgcga ctcctatagt aaacgttaaa ggaattggcc
     9721 atttatcagt tttaagataa gcgacgattg tctcgttatt gccagaggct tcttcgtcaa
     9781 gatcaacctg agtaattaaa tcgcgtcctg ttaattgtaa ctcgctacca aaattccgca
     9841 tgctggcacc aatctgaagt ccattgaatt gagttttaaa taataatccc agatcagcgg
     9901 caaatcccgt tgcgctgaca tgccagatat tttgttgaat gaatttgaca ctgcctccga
     9961 ttgaaaagcg gtcggttaag ttgcgagcat agcccaagct aaaagctaaa tcacttgctt
    10021 cccaatattc gccggttcca tccggttccg ctaccgtagt aattttttct cgctcaccgt
    10081 aatctaagta atcaacgcca attccaatga tatcattgct ggttgccatt acctgagccg
    10141 ctataaattg atgcttccct cccactaacc aattagtttg agaaacatac aatttatttt
    10201 tactaattcg ggaaattgct cccggattat aataaattgc actcgcatca tcggccagag
    10261 ctgtaaatgc gccacccatt gcaattgcct tcccaccgac tggaatttgc aaaaattgac
    10321 cggcggcagt cccttcttta ttctgagcat ttaaaaatat agtgctaatc agaactgtcg
    10381 aaattatagt aggtaatttt ctattcatgt tctcaccgct attttattac tgctaatttg
    10441 ccggattttt taccgaccga tgattctatg atatagtaat aaaccccaaa tgctacatcg
    10501 agattctctt tcgatttaac attccaggct aaagtaccat tatgaatatt gccgttgtga
    10561 tttagggtaa ttacatgcgc cccggctgat gtaaagatat gtactttggc atcgttaggc
    10621 aatttgcgaa attcaatccg cctttcccca cggccactgg taatggcagg cggtaaaggc
    10681 gattccatcg tattggcaac cacataagga ttggggacaa cctgaatatc atctaagcta
    10741 gatttagcca cttcatcatc tacattagga atttcagtag tgaattcata aatatcacca
    10801 ttacggaagg ggatttccgt ttcaatatgg agaatgtcac cttttaggaa ccggggcggt
    10861 gtaactttct ctgaaacagg cggaaagttt atcacccacg aatattgata agtgctatcc
    10921 ggtttaggaa tataagattc aatcaagcca cctggcgtta tttcataaat tccataactg
    10981 ttttttattc caccattata aagaaaaggt atctttacag aatccaacac attaaataga
    11041 taaaaattag tcttctttac cagggttctt ataattgcat ctgagaaact aggatagatg
    11101 gactttaaga attctttggg agtttcatat aatacttcgc tactatcacc gactcgcagc
    11161 tcatatattc cgggatattg tactccaact atggtatcgc cttccaataa agcctgatca
    11221 attctaaagg taaaatcaat aattctatca gtcgaattcc agcccgactt agatttaatc
    11281 attttaatat cccacggatt actaaacact aactcaacgc catcgaaaat atcggagtct
    11341 tttgtttctt tcatataagg actatactga atgtttggac ttttaaagac aggataatac
    11401 ttataaaata tacgataact accacttttt aggttactac ccgcttttat tttaattttc
    11461 ccgcgtaagg tatctatttc gaatttattt aatgataaat agttattcgg atctgactca
    11521 ctggcaatta aaatatcgtc at
//
LOCUS       DAOMJU010000068        11340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_5867_length_11340_cov_19.731148, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000068 DAOMJU010000000
VERSION     DAOMJU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5867_length_11340_cov_19.731148"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1171)
                     /locus_tag="PLO58_07100"
     CDS             complement(5..1171)
                     /locus_tag="PLO58_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HQO74593.1"
                     /translation="MDSFLKILQTTGFANIQIGNIIMIFIGLIAIYFAITKKYEPLLL
                     LPIGFGIIVGNIPSIPSIAIGVFDTVENGAASNSVFSMIYYLVTSGIFPPLIFLGIGA
                     LTDFSSLISNPKSLLLGAAAQLGIFITFIGALILGFSAPEAASIGIIGGADGPTSIFL
                     ASQLAPHLLGPIAIAGYSYIALVPLIQPPVMKLLITEKEARIRMKPARRVSRKEKILF
                     PIIVTILAALIVPGSIPLVGMLMLGNLLKESGVTDRLAKTAGTAILDTCTILLSFTVG
                     ASTQATRYVDGVNVGFLTSRSLSIFFLGFFSFVIATAGGLLFAKLMNLFLKEKINPLI
                     GAAGVSAVPDSARVVHMFAQRYDKDNHLLFHAMGPNVAGVIGSAIAAGVFLSIF"
     gene            complement(1175..1651)
                     /locus_tag="PLO58_07105"
     CDS             complement(1175..1651)
                     /locus_tag="PLO58_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQO74594.1"
                     /translation="MKEKKVELEINGQPYTVIINAITAYHASVTVNDRTYEVGLKDLG
                     FDQVADIKPKLINVPESAVPTAKPASKTPPTQLHRPKSVIDSSAVVAPLPGLIQNILV
                     KVGDTVHAGQHVLTMEAMKMENEIQTNRDGIIKDIKVRIGDSVNEGDTLIILNSGE"
     gene            complement(1653..2006)
                     /locus_tag="PLO58_07110"
     CDS             complement(1653..2006)
                     /locus_tag="PLO58_07110"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HQO74595.1"
                     /translation="MNPELLNGIIIGIGGLIIVFVGLLIIEFVIALFSQYFAKKEQKT
                     QTAAGLPSIETKKHSAQTKRIAIPEEDVVAIATTIELYRKIHFEQLPSQITFIRGNQQ
                     SNWKIGSIASQFLRK"
     gene            complement(2016..3566)
                     /locus_tag="PLO58_07115"
     CDS             complement(2016..3566)
                     /locus_tag="PLO58_07115"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HQO74596.1"
                     /translation="MSRQRKLLELKDKIELARMGGGAEAIEKQHQRGKLTARERLQLL
                     LDPNSFEEIDAFRTHSCTDFGMADKKFLGDGVVTGYGTINGRLVFVYSFDFTVFGGSL
                     SKVVAEKIVKIMEMATKVGAPLIGINDSGGARIQEGVDSLAGYTEIFVRNVLYSGVIP
                     QISAIVGPCAGGAVYSPALTDFIFMVRKSAYMFLTGPKIVKTVTHEDVTADELGGADV
                     HASKSGITHFTYDDEQSLFAGMRELLSYLPQNNMEEPPAIAPTDPVDRIDEDLNYIVP
                     ENPNKPYDMKEILAKVLDDGQFLEVQALYATNMLIGFGRLNGKVVGIVANQPRSLAGV
                     IDTNASNKAARFVRTCDCFNIPIITFEDVPGFMPGTAQEFNGIIKHGAKLLYAFAEAT
                     VPKITVITRKGYGGAFCVMNSRQLRGDYSFAWPSAEIAVMGPEAAAEIIFAHEIKTAE
                     NPEQHFNEKVEEYREKFANPYIAASKGYIDEVIEPKVTRYKLIKTLEMLSNKRDTLPP
                     KKHGNIPL"
     gene            complement(3657..4106)
                     /locus_tag="PLO58_07120"
     CDS             complement(3657..4106)
                     /locus_tag="PLO58_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HQO74597.1"
                     /translation="MDNKIHEELYKDKELTSTIIGCAYKVYKNLGFGFLESVYEKALI
                     IEMTKAGLKSESQKAIDVYYDDQIVGHFIADLFVEGKIIVELKSVATLTKSHEVQLVN
                     YLTATRINIGLLINFGEEKVEIKRKIRSLKQSNQSCKSCQKKKSQSC"
     gene            complement(4161..4565)
                     /gene="mce"
                     /locus_tag="PLO58_07125"
     CDS             complement(4161..4565)
                     /gene="mce"
                     /locus_tag="PLO58_07125"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HQO74598.1"
                     /translation="MIKKLNHIGIAVNSLTEHIPFYRDILQLNFLGIEEIADQKVKTA
                     IFQVGEIRIELLEATAPDSPIAQFLERRGEGLHHLAYQSDNIERELQFLREHNVALID
                     EKPRDGAHHTRIAFLHPKSTGKVLTEICEEKR"
     gene            complement(4570..5511)
                     /gene="meaB"
                     /locus_tag="PLO58_07130"
     CDS             complement(4570..5511)
                     /gene="meaB"
                     /locus_tag="PLO58_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQO74599.1"
                     /translation="MAEKILKGDQRSIGRAISIVENDGRDREQLIDEIFPHTNRAVVW
                     GITGAPGSGKSTLLDGLIANLRAQNRTVAVVAVDPSSPFTGGALLGDRLRMQRHATDL
                     GVFIRSMASRGHLGGIATATADTIKILDAAGYDLIVIETIGVGQAEIEVVELADIVML
                     VLVPGAGDEIQAMKAGVMEIGDIFIINKKDLDGALKLKAEVEYVLHLKSNSNESAINP
                     VLLTSAIHNEGISETLHTIDDYLARLQADGRLATRRQQRIENEVRKIVNTKIHQMAEK
                     HLQIDQKITQWAQQIGKQKQAPYALINSQINQLLKDP"
     gene            complement(5517..5921)
                     /locus_tag="PLO58_07135"
     CDS             complement(5517..5921)
                     /locus_tag="PLO58_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HQO74600.1"
                     /translation="MAKKIRILIAKPGLDGHDRGAKYIARALKDAGYEVIYTGIRQTP
                     EAIVKTAIQEDVDFVGLSLLSGAHNELFPRVVELLRENQADDIIVFGGGVIPESDIPG
                     LKAKGLAAIFTPGTPIKAVIDFIESCRSKIEN"
     gene            complement(5931..7604)
                     /locus_tag="PLO58_07140"
     CDS             complement(5931..7604)
                     /locus_tag="PLO58_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQO74601.1"
                     /translation="MPTSDKLKAQRQKWEETTLKKVISRNPERKTEFTTGSWLPVERT
                     ALPEITSDDEYLEKLGFPGEFPFTRGVQPTMYRGRLWTMRQYAGFGTAEESNRRYKFL
                     LNQGQTGLSIAFDLPTQIGYDSDDPHSEGEVGKVGVAIDTLRDMEILFDGIPLDQVST
                     SMTINAPAAILLAMYLAVAEKQGVAFDQLNGTIQNDILKEYIARGTYIFPPKPSLRLI
                     TNIFEFCARSVPKWNTISISGYHIREAGATAVQEVAFTLADGVEYVKAALNAGMDVDS
                     FADRLSFFFNAHNDLLEEVAKFRAARRIWAKIMRDKFHAQNSKSLMLRFHTQTAGSTL
                     TAQQPDNNIVRVAIQTLAAVLGGTQSLHTNSRDEALALPSEDSVRIALRTQQIVAYES
                     GVTNSVDPLGGSYLIENLTDRIESEVWQYLDQIEAIGGMIPAIENGFIQKEIQDAAYR
                     YQKEIESNERIIVGVNKFTTDEETPKNLLKVDSELQRIQIQKTDAVKKQRNNQAVKEK
                     LELLRETARRTEQNLMPPILAAVKEYATLGEICGILRQEFGEYQESIVL"
     gene            complement(7822..9486)
                     /locus_tag="PLO58_07145"
     CDS             complement(7822..9486)
                     /locus_tag="PLO58_07145"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQO74602.1"
                     /translation="MVNESSMASAGLVPIRQIAEKIGIPSEAMDLYGDYKAKIKLSYF
                     NQLPPNAKNKLILVSAMTPTPAGEGKTTVAISLAMGLNRIGKRAIVTLREPSLGPLFG
                     MKGGATGAGKAKVLPVEEINIHFTGDIHAVTAAHNLIAACLDNHIFRRKEPIIDPRRV
                     TLPRVMDMNDRALRNIIVGLGGKSNGIPRETGFIITAASEIMAILCLVKSYSEMQEKL
                     NRIILGYTYDSEPVCLGDLKITGAVASLLKEPLKPNLVQTSEGTPAFVHGGPFANIAQ
                     GTCSIMSMKMAAGLADFVVTEAGFGFDLGGEKFFDIVCRMNGLNPQAVVLVATIRALK
                     MHGGKSKSELDIPDLEAIRKGFENLEKHLENLAFFGIPGIVALNRFATDTEAEISLLN
                     ELVASKGYPFAVVDSFAKGSAGAIELAEKVAEIVQSERHYTPLYELNTPVIEKINTVC
                     QKIYGAEEVEYTPEARADLQQIKNLGLENLPICIAKTQNSLSDNPNLIGRPRNYTLTV
                     RQIGISAGAGFLVPVTGNILLMPGLPTKPVAENVTIDDDGNISGLF"
     gene            complement(9530..10081)
                     /locus_tag="PLO58_07150"
     CDS             complement(9530..10081)
                     /locus_tag="PLO58_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQO74603.1"
                     /translation="MLRDILDRRRATRSLAPVEITQAMIKDLAESARLMPSCFNKQPW
                     RFIFVYDKEQLTRLHPALNKGNEWAKKASLIVAVFTKKDLDCVIKDREYYLFDTGMAT
                     AALILRATEMGLVAHPIAGYDEDKVKEALNIPTEYQVITLINIGKQADEIDPILSDYQ
                     KLAEKERPARLPLENFVYLNKFS"
     gene            10068..11324
                     /locus_tag="PLO58_07155"
     CDS             10068..11324
                     /locus_tag="PLO58_07155"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO74604.1"
                     /translation="MSLSITNLPILIFCIFTCFARLSMVTFREDNEINCYIAMTGEHK
                     IVTLLIVYFSLISLYSIPGNANEWQEQKIQQALFEMYNLNNVAAHQHFKEVLNSQPFH
                     PLAPMAELATDWLKNQEELGYQEGNRILASRIDSVLAIYHDKLKANPQNGELEFYYGC
                     TYGLKARLSLGEKDYLGVLINGYNAVRYIKAAERHQPDFIELQIPFGVFNYYVGISRG
                     YMQIASWIMNTSGSKEEGLAQMRYAAHNARYSCYEARSILAMVTLYFEGDYESTLYYA
                     EMMASDFPNNPYYNYLAGEALIQMGRYAEAEKQIKRIKQLLPKLKPKTQKEYWSKLHQ
                     LQGSLALKKGDLATAEKELRAVIDNYDQEMDVQLGFALLRLGNVYDLTGRRELAVQCY
                     QRAYGLKNRSQACQLAKLYLKTPYTQ"
BASE COUNT         2968 a         2497 c         2651 g         3224 t
ORIGIN      
        1 attgctaaaa tattgacaga aaaaccccgg cggcgatagc ggaaccgatt acgccagcga
       61 cattcggacc catagcgtga aacagcaggt gattgtcttt atcataacgc tgagcaaaca
      121 tatgcacaac ccgcgctgaa tcgggaactg ccgaaacgcc agccgctccg atcaatgggt
      181 tgattttttc cttcagaaac aaattcatca atttggcaaa taataacccg ccagccgtag
      241 cgatcacaaa agaaaagaag ccaagaaaga aaatactaag tgagcgagac gtcaaaaatc
      301 caacgttgac gccatcaaca tagcgcgtgg cttgagtact agccccgacc gtaaaggata
      361 agagaatagt gcaggtatct aggatcgccg ttccggctgt ttttgccaga cgatcggtga
      421 cgccgctctc tttcaataaa ttgcccagca tcaacatacc cactaacggg atagaccccg
      481 gtacgatcag agcggctaaa attgtgacga taatcgggaa gaggattttc tctttacggg
      541 aaactctgcg ggctggcttc atccgaatgc gcgcctcttt ttcggttatc aatagtttca
      601 taaccggtgg ctgaattagt ggaacgagtg cgatatacga ataaccagcg attgcgattg
      661 gtcccagtaa atggggcgcg agttgggagg cgagaaagat cgaagttggt ccatctgctc
      721 ctccaatgat gccgatcgag gcggcttccg gcgcagaaaa accgagaatc aacgcaccga
      781 taaaagtaat gaaaatacct aactgagcgg cggctcccag tagaagtgat ttcggattag
      841 aaattaatga tgagaaatcg gttaaggctc cgatgcccag aaaaatcagc ggtggaaata
      901 ttcccgaagt cactaaataa taaatcatac tgaaaacgct gttactcgcc gcgccattct
      961 caactgtgtc gaagacccca attgcaatag atggtatgga aggaatattg ccgacaatta
     1021 taccgaaccc aatcggcaaa agcaggagcg gttcgtactt tttcgttatc gcaaagtaaa
     1081 tggcaatcag cccgataaaa atcataataa tattgccgat ctgaatatta gcaaagccag
     1141 tcgtttgtag aattttcagg aaactatcca tttatcactc ccctgaattg agaattatga
     1201 gggtatcgcc ttcgttgacc gaatcgccga ttcggacttt aatgtctttg ataatcccgt
     1261 cacggttagt ctggatttcg ttttccattt tcatggcttc catcgtcagg acgtgctggc
     1321 cggcgtggac ggtatccccg actttaacta atatattttg aatcagaccc ggcaagggtg
     1381 ctaccaccgc ggagctgtcg atcaccgact taggccgatg cagttgagta ggcggagtct
     1441 tacttgccgg tttagcggtt ggtacggctg attcaggaac attaattaat ttaggtttaa
     1501 tgtcagcaac ttgatcgaag cccagatctt ttaagccaac ttcgtaagtg cgatcattga
     1561 cggttacgga agcgtggtag gcggtaattg cattgatgat gacagtgtaa ggttgaccgt
     1621 ttatctctaa ttctaccttt ttctctttca tactatttcc tcaagaattg actggcgatt
     1681 gagccgattt tccaattgct ctgctgattg ccacgtataa aggtaatctg gcttggtaac
     1741 tgctcgaagt ggattttgcg ataaagttcg attgtcgtag cgatggcgac cacatcttct
     1801 tccgggatgg cgatgcgttt ggtttgcgcc gaatgttttt tagtttcgat tgacggcaaa
     1861 ccggcagccg tttgggtctt ctgttctttt tttgcaaaat actggctgaa cagagcgata
     1921 acgaattcga taattaacaa tccgacaaat acgataatca gtccaccgat tccgattatg
     1981 attccattca atagttccgg gttcattgct tgctcctata aaggaatatt gccatgtttc
     2041 ttaggtggga gagtatcgcg cttattagaa agcatctcca gcgttttgat caatttgtag
     2101 cgagtaactt taggttcaat cacttcatca atgtagcctt tggaggcggc gatgtaggga
     2161 ttggcaaact tttcgcgata ttcttcgact ttttcgttaa aatgctgttc cgggttctcg
     2221 gcggttttaa tttcatgggc gaagatgatt tccgcggcgg cttccgggcc cattacggca
     2281 atttcggcac tgggccaggc gaagctgtaa tcgccgcgca gttggcgcga attcatgacg
     2341 caaaatgcgc cgccatagcc cttgcgggta atgaccgtga ttttgggcac ggttgcctcg
     2401 gcaaaagcat agaggagttt agcgccgtgc ttgataatgc cgttgaattc ttgggcggtg
     2461 cccggcataa atcccggcac atcttcgaaa gtaatgatgg gaatattgaa acaatcgcag
     2521 gtgcgcacga aacgggcggc tttgttcgac gcgttagtgt caatgacgcc cgccagcgat
     2581 cggggttgat tggcgacaat gccgacgact ttgccgttta aacgtccgaa cccgattagc
     2641 atattggtgg cgtacaaagc ctgaacttcg aggaattgtc cgtcatccag aactttggcg
     2701 agaatttcct tcatatcgta aggcttattg ggattttcgg gcacgatata attcaagtcc
     2761 tcatcaatgc gatcaaccgg atcagtcggt gcaatagccg gcggttcttc catattgttt
     2821 tgcggtaaat agctcagcag ttcgcgcata ccggcgaaca gactttgttc atcgtcatag
     2881 gtaaaatgag taatgccgct tttgctggcg tgaacatcgg cgccacccaa ttcatcggcg
     2941 gtgacatcct cgtgagtaac ggttttgact atttttgggc cagttaaaaa catataggct
     3001 gattttctaa ccataaaaat aaaatcggtc aaagctggtg aataaactgc tccgccggcg
     3061 caaggtccga cgatggcgct gatctgggga atgacgcccg agtaaagcac attgcgcaca
     3121 aaaatttcgg tataaccggc cagtgaatcc accccttcct gaattcgcgc gcctccggag
     3181 tcattgatgc cgattaacgg agcaccgact ttagtcgcca tttccataat tttgacaatt
     3241 ttttcagcta cgacttttga tagcgaacca ccgaagaccg tgaaatcaaa agaataaacg
     3301 aataccagac gaccattgat agtaccataa ccggtgacga ctccgtcgcc gagaaatttc
     3361 ttgtcagcca taccgaagtc ggtgcaggaa tgagtgcgaa aggcatctat ttcctcaaaa
     3421 ctattcggat cgagtagtaa ttgcaggcgc tcccgggccg ttaacttgcc gcgctgatgc
     3481 tgtttttcga tggcttccgc accgccgccc attctggcaa gttcaatttt atcttttaat
     3541 tcaagtagtt tacgttgacg actcatttat gctccttgta tattttattg acaagattaa
     3601 cattgttttt attttagaca agattaacaa gattgatttt ttttctttag acaagattaa
     3661 caagattgac tttttttctt ttgacaagat ttacatgatt gatttgattg ttttaatgat
     3721 cttattttgc gtttgatttc gactttctcc tctccgaaat ttattaatag tccgatattt
     3781 attctggtag ctgttaagta attcactaat tgaacttcgt gacttttcgt caaagtcgct
     3841 acagatttta attcaacgat tatttttcct tctacaaaaa gatcggcaat aaaatgtcca
     3901 acaatttggt catcataata gacatcaatt gctttctgag attcggattt caaacccgct
     3961 ttggtcattt caattatcag agccttttca tagacggact ctaaaaagcc gaatcccagg
     4021 tttttataga ccttgtaagc acaaccgatt atggtggatg tcaattcttt atccttgtat
     4081 aactcttcgt gtattttatt atccatctta gttctaaata ttaataaaca acatgtaaat
     4141 cttgttaatc ttgtctaaaa ctacctcttt tcctcacaga tttcagtcaa gaccttgcca
     4201 gtggatttcg ggtgcaggaa agcgatgcga gtatgatgcg cgccgtctct cggcttttcg
     4261 tcaatcaagg caacattatg ttctcttaaa aattgcagtt ctcgttcgat gttgtcgctt
     4321 tggtaagcca gatgatgcaa accttcacct cttctctcga gaaattgggc gattggacta
     4381 tcgggcgcag tggcttcgag taattcgatc cggatttcac cgacttgaaa gatggcggtt
     4441 ttgactttct ggtcggcaat ttcttcgatg ccaaggaaat ttaattgcaa aatatcccga
     4501 taaaacggta tatgttccgt aagagagttg actgcgatgc cgatgtggtt gagttttttt
     4561 atcatagaat taggggtcct ttaataattg attaatttga gaattgatca aggcataagg
     4621 cgcttgcttt tgttttccga tttgttgagc ccattgcgtg attttctggt caatttgcag
     4681 atgtttttca gccatttggt ggattttggt gttgacgatt tttctgactt cgttttcgat
     4741 ccgctgttga cggcgagttg ccagccgtcc gtcagcctgc aatcgggcaa ggtaatcgtc
     4801 aatagtatgg agtgtttccg atatgccttc attgtgaatc gctgaagtta atagaaccgg
     4861 atttattgcg gattcgttac tatttgactt gaggtggagg acatactcga cttcggcttt
     4921 gagtttgaga gcgccgtcga ggtctttctt attgataatg aaaatatcac cgatttccat
     4981 cactccggct ttcatcgcct gaatttcatc acccgctccc ggtaccagaa cgagcataac
     5041 gatatcagcc agttcgacga cttcgatttc agcttgcccg acaccaatag tctcaatcac
     5101 gattaaatca tagccggcgg catccaaaat tttaatagtg tcagccgtgg cagtggcaat
     5161 gccacctaaa tgtccgcgcg aagccatcga gcggatgaaa acgcctaaat cggtggcatg
     5221 tctttgcatg cgtaaacgat caccgagaag cgcaccgcca gtaaacggtg aactgggatc
     5281 aaccgcgact accgccaccg tccggttttg agcccgcaaa ttggcgataa gaccatccag
     5341 caaagtgctt tttccactgc cgggtgcgcc agtgattccc caaactactg cccgattggt
     5401 gtgcgggaaa atttcgtcaa ttaattgttc gcggtccctg ccgtcatttt ctacaataga
     5461 aatggcacga ccgatgctcc gctggtcgcc tttcaaaatt ttttcagcca attcgctcaa
     5521 ttttcgattt tgctccgaca ggattcaata aaatctatga cagctttaat tggcgtcccg
     5581 ggtgtaaaaa tggcggctaa tccttttgcc tttaaaccgg gaatatcact ttctgggata
     5641 acgccaccgc cgaaaacaat gatgtcgtca gcctgatttt cacgtaaaag ttcgacaact
     5701 cgcgggaaaa gctcattgtg agccccggac aacaggctga gaccgacgaa gtcaacatct
     5761 tcctgaatag cagttttgac aatggcttcc ggtgtctgac gaattccggt gtagattacc
     5821 tcatatccgg catctttcag agcgcgggca atgtacttgg cgccgcggtc gtgtccatcc
     5881 agcccgggtt tggcaattaa tattctgatc tttttagcca tattgaatcc ttataaaacg
     5941 attgattcct ggtattcgcc gaattcctgg cgcagaatgc cacagatttc gcccagtgtg
     6001 gcatattcct taacggcggc gagaatggga ggcataagat tctgctccgt tcggcgcgca
     6061 gtttctcgta aaagttcaag tttttcctta actgcttgat tgttgcgttg ttttttgacg
     6121 gcgtcagttt tttggatttg gatgcgctga agttcggaat caacttttaa taaatttttc
     6181 ggtgtttctt cgtcggtggt gaatttattg acgccgacga tgatgcgctc gttgctttcg
     6241 atttcttttt ggtagcggta agccgcatcc tggatttctt tctggataaa gccattttca
     6301 atagccggaa tcatcccgcc aatggcttca atttgatcga gatattgcca gacttcgctt
     6361 tcgatgcgat cggtaagatt ctcaatcaaa taggagccgc ccagtggatc aactgaatta
     6421 gtcacgccgg attcgtaggc gacaatttgc tgagtgcgta gagcaatgcg cacggaatct
     6481 tcggacggta aagccagagc ctcatcgcgg gaattggtat gcagactctg ggtgccgcca
     6541 agcactgccg ctaatgtttg gattgcaacc cggacgatat tattgtccgg ctgttgggcg
     6601 gtgagagtac taccggccgt ttgagtatga aagcgcaaca ttagagactt cgagttttgg
     6661 gcgtggaatt tatcgcgcat aattttagcc cagatgcgcc gtgccgcgcg gaatttggcg
     6721 acttcctcca acaaatcgtt gtgggcatta aagaaaaagg agagccggtc ggcaaaacta
     6781 tcaacgtcca ttccggcgtt caaagcggct ttgacgtatt caacgccatc agccagagtg
     6841 aaagccactt cctggacggc agtcgcgccg gcttcgcgaa tatgatagcc ggaaatgcta
     6901 atcgtattcc atttcgggac tgaacgagcg caaaattcaa aaatattagt aattaaacgt
     6961 agagacggct tgggcggaaa aatataggtg ccgcgggcta tgtattcttt cagaatgtcg
     7021 ttctggattg tgccattgag ctggtcgaag gcgacacctt gtttttcggc tactgctaag
     7081 tacattgcca gtaaaattgc ggcgggagca ttaattgtca tcgaagtgga aacttggtct
     7141 aaaggtatgc cgtcgaaaag gatctccata tcgcgcagag tatcaatcgc tacgccgact
     7201 ttaccgactt cgccttcgct atgcggatcg tcggaatcat agccgatttg agtcggcaaa
     7261 tcgaaggcga ttgataagcc ggtttgtccc tgattaagca gaaatttata acgccggtta
     7321 gattcttcgg cagttccgaa tccggcgtac tgtcgcattg tccagagacg accgcggtac
     7381 atcgtgggct gaacgccgcg cgtgaaggga aattcaccgg ggaagccgag tttttccaga
     7441 tattcatcgt cggaagtaat ttccggcaga gccgtgcgtt cgaccggtaa ccatgagccg
     7501 gtggtgaatt ccgtttttct ttcaggattg cgagagatga ctttttttag agtagtctcc
     7561 tcccactttt ggcgctgtgc ttttaattta tcggaggttg gcatagattg ctcctgatta
     7621 attattattg acactggtta tggtatggag aagaaatgat tgtattgtga gcgacattaa
     7681 cgttacatct cgctgtcaaa atgacttaaa aaattaaaga ggaatcagga gaaaaacaag
     7741 atggctggca agattggaag ttgggtaaat tatttgggct attaaagcga gaaattaaaa
     7801 gctaaagata ttgtagttcg attaaaacag tccgctgata ttgccgtcgt catcaatggt
     7861 gacgttctcg gcgactggtt tagttggcaa gccgggcatc aacagtatat tgccagtcac
     7921 aggcactaaa aaacccgctc cggcggaaat gccgatctgc cttacggtta gagtataatt
     7981 gcgcggtctc ccgatcaagt ttggattatc agagaggctg ttctgggttt tggcgataca
     8041 gataggcaga ttctcgaggc caaggttttt aatttgttgc aaatcggcgc gagcttccgg
     8101 ggtgtattcg acttcttctg caccataaat cttttggcaa actgtattga ttttttcaat
     8161 cactggggta ttcagctcgt ataaaggcgt ataatgccgc tcgctttgaa caatttcggc
     8221 gactttttca gccagctcaa tcgccccggc gcttcctttt gcgaatgaat ccacgacagc
     8281 gaatgggtat cctttggagg caactaattc atttaataga ctaatttctg cctcagtatc
     8341 agttgcaaat cggtttagag ccacaattcc cgggatgccg aagaaagcga gattctcgag
     8401 atgcttttct aaattttcaa aaccttttcg aatggcttca aggtcaggaa tatccagctc
     8461 ggatttagat ttaccgccgt gcatcttgag tgcccgaatc gtagccacta acacgactgc
     8521 ctgtggatta agaccgttca ttcggcagac aatatcgaaa aatttctcac cgccgagatc
     8581 gaacccaaaa ccggcttctg taacgacgaa atctgccaat ccagccgcca tcttcataga
     8641 cattatcgag caagttccct gcgcaatatt ggcaaacggt ccaccatgca caaaagccgg
     8701 agtgccttcg ctggtctgta ccaggttggg tttcaagggc tcttttaata aagatgcaac
     8761 cgctccggta atttttaaat cgcctaaaca caccggctcg ctgtcgtaag tatagcccag
     8821 gattatacga ttaagttttt cttgcatttc ggaatacgat tttaccaagc agagaatcgc
     8881 cataatttcg gaagcggcgg ttataatgaa tcccgtctca cgaggaattc cattagattt
     8941 gccgcccaaa ccgacgatga tgtttcgtaa agcccgatca ttcatatcca taacccgtgg
     9001 caaggtaacc cgtcttggat caattatagg ctccttgcga cggaaaatgt gattatcgag
     9061 acaagcggca atcaagttgt gagcggcagt tacggcatgt atatcaccgg taaaatgaat
     9121 attgatctct tcgactggaa gtactttggc tttgccagcg ccggtcgcgc cgcctttcat
     9181 tccaaaaagc ggacccagcg atggttctct caatgtaaca atcgcccttt ttccaatgcg
     9241 gttcagtccc attgccagac tgattgctac ggttgtctta ccttcaccag ccggagttgg
     9301 cgtcatagcc gagaccagaa ttaatttatt tttagcgttg ggagggagct gattgaagta
     9361 actcagcttg attttggctt tgtaatcacc atataaatcc attgcttcgg aagggattcc
     9421 aattttttcc gcaatttggc gaattggaac aagacctgct gaagccatcg aagattcatt
     9481 gaccataacg cgcctatatt agttaattaa tttaatcaac ggctatatgt taactaaatt
     9541 tatttaaata aacaaaattt tcgagaggca agcgcgccgg tcgttctttt tcggctaatt
     9601 tttgataatc gcttaaaatt ggatcaattt catcagcttg tttaccgata tttatgagag
     9661 taattacctg gtattcagtg ggaatattca aagcctcctt gactttatcc tcatcgtaac
     9721 cggcaatggg atgggcgacc aatcccattt cggttgcgcg caggatgaga gccgcagtcg
     9781 ccattccggt gtcgaataga taatattctc gatctttgat aacgcagtcg aggtcttttt
     9841 tagtgaaaac cgccactatt agcgaagcct ttttagccca ttcattgcct ttgttaagag
     9901 ctggatgcag gcgagtcaat tgctctttat cataaacaaa aatgaatcgc caaggctgtt
     9961 tattaaaaca agacggcatt aaccgggcac tttccgccaa atcctttatc atcgcctggg
    10021 tgatttcaac tggcgctaat gagcgcgtgg cacggcgtct gtctaaaatg tctctcagca
    10081 taacaaacct ccctatttta atcttttgca ttttcacttg ttttgcacgc ctttcaatgg
    10141 ttacctttag agaagataat gaaataaatt gttacatagc aatgactggt gaacataaaa
    10201 ttgtaacgct cttaattgtt tatttttctt taatcagttt gtactcaata ccgggtaatg
    10261 ccaacgaatg gcaggaacaa aaaatccagc aagccttatt tgagatgtat aacctgaata
    10321 acgtcgccgc gcaccagcat tttaaagaag tattaaatag ccaaccgttt catccgttgg
    10381 cgccgatggc agaactggca actgattggt tgaagaatca ggaagaattg ggctatcagg
    10441 agggcaatcg aatcctggct tcgcggattg actccgttct ggcaatttat catgataaat
    10501 taaaagctaa cccgcagaac ggcgagctgg aattttatta tggttgtact tacggtttga
    10561 aagcccggtt gtcgctcggt gaaaaggatt acctgggtgt cttgattaac ggctacaatg
    10621 cggtacgcta tatcaaggcg gcggaacgtc atcaaccgga tttcattgag ctccaaatac
    10681 cttttggcgt gttcaattat tatgtgggta tttcgcgagg ctatatgcaa atcgcctcct
    10741 ggattatgaa tacgtcggga agtaaagagg agggattggc gcagatgcgt tatgccgctc
    10801 ataatgcgcg ttacagttgt tacgaagcgc gcagtattct ggcaatggta actttatatt
    10861 tcgaaggtga ctacgagtcc acactctact atgcggagat gatggcgtcc gactttccca
    10921 ataatccgta ttataattac ctagctggtg aggcgctgat tcaaatgggg cgctacgccg
    10981 aggctgaaaa acagattaag cgaatcaaac aattattgcc gaaattaaag cccaaaacgc
    11041 aaaaggagta ttggtcaaaa ttgcaccaat tgcagggcag tctggcgctt aagaaaggtg
    11101 atctggcgac agctgagaag gaacttcggg cagtgattga taattatgac caggaaatgg
    11161 atgtccagtt gggatttgcg cttcttagat tgggcaatgt ctatgacttg actggtcggc
    11221 gggaattggc ggttcaatgc taccagcgag cgtacggatt gaagaaccgc tcgcaagcct
    11281 gccaattagc caaactttat ttgaaaacac cctatactca ataaaaaggc tgagactgag
//
LOCUS       DAOMJU010000069        11077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6046_length_11077_cov_20.273544, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000069 DAOMJU010000000
VERSION     DAOMJU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11077
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6046_length_11077_cov_20.273544"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PLO58_07160"
     CDS             complement(<1..1475)
                     /locus_tag="PLO58_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74605.1"
                     /translation="MIDIKERTELTNVLVRKFGEENRDRLETGVKQVCDFWRAEDGDL
                     ATLKQFCLDNFISDQAELLKAFEHIEKTFETMSGVLVELNRDLRWYMDVPTGPILPID
                     YLLANFNLGSHVEDDLYATKIAFFVLLNYKVYTLEEILAEGAEWDRQKWAEVRLAQKF
                     TSRVPAQYNQKRYEAYTAADNYITNYNIYMHNLLTEDEQRLFPEGLKLIAHWGLRDEL
                     KAQYQLSDGFPRQKLIYEVMLKIINQEIPKIVIDNPAVDWNVAKNTVSGVSSDNSPEP
                     DTRYARWLGVFQGERACDPYYLISRSQIDRRFNEDREIPEDIVRQLFVDVLTAPELKQ
                     TARLISKRLGRELEPFDIWYNQFQAKSRYTEAELDKITQVKFPTAESFKMQLPEILMK
                     LGFDRSTANFLQTKIDVDPARGSGHAMGAARRSDNAHLRTRVTENGMDYKGYNIALHE
                     LGHCVEQVFSLNKVDHTLLQGVPNTAFTEAFAFVFQKRDLDV"
     gene            complement(1598..2050)
                     /locus_tag="PLO58_07165"
     CDS             complement(1598..2050)
                     /locus_tag="PLO58_07165"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQO74606.1"
                     /translation="MSLIKKYFSTSDLDAIKKACECAEKNTAGEIRVSIFEKRPPKTS
                     KMSLPELAYAEFKNLRMDQTRDRTGILLFILLAERQFQILADEGINAKVEQEVWDDIA
                     EQMAEKFKNGDYLTGVVSAVKRIGEILAQWFPRKPDDINELSNEVHIS"
     assembly_gap    2091..2190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2191..2718)
                     /locus_tag="PLO58_07170"
     CDS             complement(<2191..2718)
                     /locus_tag="PLO58_07170"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HQO74607.1"
                     /translation="MLKIKFRNWRIPALVGLLLFNLKAFGLDVPPLKRHVTDLAGILT
                     PSELNRLEENLIAFENETSNQIAVLIINSLEGEPIEQYSIRVADQWKLGTADRDNGVL
                     LLIAVQDRQMRIEVGYGLEGVLTDLISSQIIRNVIAPEFNKGNYYAGIDQGLRAIMMA
                     TKNEYQADPRQREQYQ"
     gene            complement(2728..3321)
                     /locus_tag="PLO58_07175"
     CDS             complement(2728..3321)
                     /locus_tag="PLO58_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQO74608.1"
                     /translation="MKKGWVILIAVVVVVLLIVGAVINIYNNLVSLEESVNQAWAQVS
                     NQYQRRADLIPNLVETVKGYAAHERETLESVIQARANATSLNVTPEVLNDPEAFAKFQ
                     QVQGELSTALSRLMAVAENYPNLKANENFLALQSQLEGTENRIAVERQRFNQAVQTYN
                     TRIRRFPNNIFAGMFGFEKKMYFEAQEGAEVAPKVEF"
     gene            3532..4614
                     /gene="ychF"
                     /locus_tag="PLO58_07180"
     CDS             3532..4614
                     /gene="ychF"
                     /locus_tag="PLO58_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQO74609.1"
                     /translation="MQIGITGLPYSGKSTLFATLLSRKSESDPFHKKETEKGIVKVPD
                     VRLDRLTEIFNPRRKVPATIEYLKVPGLESQSSAAKGLPPQFLANLKTVDVILLIVRA
                     FDNPLYPHPLNRIDPQGDINFVNSEFLLNDLAIVETRIERLEKQLSKMHNLSFQKELE
                     VLFKCRNFLEDEKPLRSLALLPEEDKILRGFQFLTAKPLIIVLNIDEKEIQQADEITE
                     RYANLKTVNTTYTVLCAELEKEISELDPAEQPLFLTELGISEPALNRLIRLSYELLGY
                     ISFFTVGEDECRAWTIINGDNAQKAGRAIHSDIERGFIRAEVVHYTTLLENGNLSTCR
                     AKGLIRSEGRDYIVRDGDVIEFRFNV"
     gene            complement(4737..5438)
                     /locus_tag="PLO58_07185"
     CDS             complement(4737..5438)
                     /locus_tag="PLO58_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQO74610.1"
                     /translation="MMSNNKLLVLIDIDGTLIKSGLTPRKALKQAIFEITGCPVTFED
                     NHLAGLTDPLIITNTLNQLGLGLKDDGLPTKILNRYLEIFSWDYPAAPDKKIYPGAIE
                     LLDYLKTLPVRMGLLTGNAKRGAQIKLAPFGLWRYFDFGVFGDDNANRNELPRLALRK
                     VHELFSESYPPNKVIIIGDTVHDLICAKVNQMQSVIVVRYPEWRSSIEAGKPELLIES
                     FVPLEPVQNWFEKLL"
     gene            5691..6386
                     /locus_tag="PLO58_07190"
     CDS             5691..6386
                     /locus_tag="PLO58_07190"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQO74611.1"
                     /translation="MPKDFELIIFDMDGTLYPVSAEMDSIYPIVALECAAKSVNRQPA
                     EIKADFEQKRAELARYLNGIPTSTLTLMYFYDADMHAFEMEITRRILTKKYVQPDART
                     IRVIDAIAQFYPIFLFTTNNRLTAEKILDQIRLSEYFPPERRFTFSDINALPISKTEK
                     INFMKPSTKGFEHIMEKCGANPNRTLMVGDSETMDIEPARRLGMEIYHVTEPALLYAL
                     PEWLEIEHNDKKI"
     gene            6679..7557
                     /gene="dapA"
                     /locus_tag="PLO58_07195"
     CDS             6679..7557
                     /gene="dapA"
                     /locus_tag="PLO58_07195"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQO74612.1"
                     /translation="MFKGLGTALITPFTENGEVDYDAFRKLIHYQIDGGADCLIVLGT
                     TGEAPAILSHEREKIVQIAIAETNHRIPVIIGTGSNCLDTVLKYNRMAEELGSDGLLI
                     VTPYYNKGTQESIFQYYRYIAERTKLPIIVYNVPSRTGFNLLPDTMFRIFNACPNIIG
                     IKEANADFGQLTKLMAGRPEGMLVYSGNDDITLPFMALGAEGVISVVGNIIPSEMKQL
                     TVRMAADDLAGAREIQNRYLDLINAMFVEVNPIPVKYAASRLNLCQNVLRLPLIPLSE
                     KNAQLVASLMQAKGLI"
     gene            7554..8201
                     /locus_tag="PLO58_07200"
     CDS             7554..8201
                     /locus_tag="PLO58_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQO74613.1"
                     /translation="MKYGIIGFSGRMGHEIDELFREKGHECVLLIDDKTEKVESVPEV
                     ILDFSLPSGIEKTITNVRKYQCALITGVTGYSAEQLEAIRALGQEVPVVQSFNFSLGI
                     QLMLKMVNLLKEPLKDWDVEIAETHHRFKKDKPSGTAKMLAATIGREVPTSSFRLGNV
                     LGDHSIAFGSLGEIITLSHRTLSRRAFAEGALLSAEFAIRQKSGYFTFTDVLSQD"
     gene            8504..9082
                     /locus_tag="PLO58_07205"
     CDS             8504..9082
                     /locus_tag="PLO58_07205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQO74614.1"
                     /translation="MDKNTHELFEVTLSETRSHVLASTTNGIVVEMIERLGEIDFGRI
                     VKISETLVDNFGQNARFNRNMIKKYFNYPETLPFVIRYNGEIEGFIIGVPLEYFDKES
                     WAQCDENLGKNNTIYTYAFIVRKEHRALGLSKMLKRVYQNTLKRRGFLYITGHVMQGV
                     TANFTRDYKIVRQFENWNNTGFTFDYYRSALK"
     gene            complement(9129..10004)
                     /gene="galU"
                     /locus_tag="PLO58_07210"
     CDS             complement(9129..10004)
                     /gene="galU"
                     /locus_tag="PLO58_07210"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HQO74615.1"
                     /translation="MIKKAVIPAAGLGTRFLPATKAQPKEMLPIIDTPTIQYVVQEAI
                     DSGIDDILIISGKGKRAIEDHFDRNTELEHILEQKGESDLATSISRIGEMANIHYIRQ
                     KQLNGLGDAIYYARQHTGDEPFVVMLGDTINDAVIPVTQQLIDIYNQYHQTIIAVERV
                     PLDKIQMYGIVSGDEVSERVYKVNQLIEKPRPTQTRSNLAVAGRYILTPTIYSMLEKV
                     KPGKNNEIQLTDAIQLLLQRESVYAFIFEGKRYDVGNKLDYLKTTVEMALKREEFAGQ
                     FLDFLKSIVAQAEKK"
     gene            complement(10030..11025)
                     /locus_tag="PLO58_07215"
     CDS             complement(10030..11025)
                     /locus_tag="PLO58_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HQO74616.1"
                     /translation="MIIETHAYARAGLLGNPSDGYFGKTISIIVANFGAHVLLYETPE
                     LKIESSDSDRNVYKNAYDLVERIGSLGYYGGDRLIKAAIRKFFTYCMENNIKLDYRNF
                     SIRYNSSIPRQVGLGGSSAIVTATMRALMKFFKVTIPKELLPSLILAAEVDELGINAG
                     LQDRVIQVYEGCVYMDFNREIMEQKNHGIYIPLDKSKLPKLYLAYKTSLSKVSGQSLN
                     DIKVRYERGDPEVIACLSRIAELAEEGKAAIENGDEATLNRLINENFDNRKKIMTISE
                     SNQEMIDVARKCGASAKFAGSGGSIIGIYKDDAMLNRLIVNLKKINARVIKPYIV"
BASE COUNT         3141 a         2396 c         2255 g         3185 t
ORIGIN      
        1 acatccaagt cgcgcttctg gaaaacgaaa gcaaaggctt ccgtaaaagc cgtgttcggt
       61 acaccctgca ataaagtatg atcaacttta ttgagcgaaa aaacctgctc gacgcaatgt
      121 ccgagctcat ggagagcaat attataacct ttgtagtcca taccattttc cgtgactctc
      181 gtgcgcagat gcgcattatc cgaacgacga gcggctccca tagcatgtcc agaaccgcgg
      241 gcagggtcaa cgtcaatctt agtttgcaaa aaattagctg tactccggtc gaaaccgagt
      301 ttcatcagga tttccggcaa ttgcatttta aaagattcag ccgttggaaa tttcacctga
      361 gtaattttat caagttcggc ttccgtgtat cttgatttag cctgaaattg gttataccag
      421 atatcgaacg gttccaactc gcgaccaagc cgcttggaaa tcaaccgcgc ggtttgctta
      481 agttccggcg cggtcaatac atcaacaaag agttgccgga cgatgtcctc gggaatttcg
      541 cggtcctcat tgaaacgacg atcaatttgc gatcgggaaa ttaggtaata cgggtcgcag
      601 gctctttcgc cttggaaaac gcccaaccac ctggcgtagc gcgtatccgg ctcaggtgag
      661 ttatcagagc tgacaccgct aaccgtattt ttagcgacat tccaatccac cgccggattg
      721 tctatcacaa tctttggaat ttcctgattg atgattttca acattacttc ataaatcaat
      781 ttttgccgag ggaaaccgtc agatagctga tactgcgcct ttaattcatc acgcaaaccc
      841 caatgcgcaa ttaatttcag cccctcagga aaaagtcgct gttcatcctc cgtcagcaga
      901 ttgtgcatat aaatattata attcgtgata taattatcgg cggcagtgta ggcttcatag
      961 cgtttctgat tatattgtgc cggaacgcgc gaagtgaatt tctgcgctag acggacttct
     1021 gcccacttct gccggtccca ctcggcacct tcagcaagaa tctcttccag agtataaact
     1081 ttatagttca gtaaaacaaa aaacgcaatt ttggtagcgt ataaatcatc ttcgacatga
     1141 ctgccgagat tgaaattagc cagtaaataa tcaatcggta gaataggacc ggtcggcaca
     1201 tccatatacc atctcaaatc acgattcaat tctaccagaa caccgctcat cgtttcaaaa
     1261 gttttttcga tatgttcaaa agctttcaat agctcagcct gatcggaaat aaaattatcc
     1321 agacaaaatt gcttcagagt ggcgaggtcg ccatcttcag cccgccagaa atcgcaaact
     1381 tgcttgacgc cagtttcaag gcggtcgcga ttttcctctc cgaattttcg gactaaaacg
     1441 tttgtcaact cggtgcgctc tttgatatcg atcattttag cctccggttt tttctcaccg
     1501 caattatgca aaaagcttaa tgaaaaggat aaaaataaac caaaaaatat gaggtttaac
     1561 tttgacaatc tcattttaat tcctcttatt attaaactta agagatatga acttcattac
     1621 tgagttcgtt gatatcgtca ggcttgcgcg gaaaccactg ggcaaggatc tcgccaattc
     1681 gttttactgc ggatacaact cccgtaagat aatcgccgtt tttaaatttt tcagccattt
     1741 gttcggcaat atcatcccag acttcttgct cgactttagc attaataccc tcgtcggcta
     1801 aaatctggaa ctggcgttcg gctaataaaa taaacagcaa aattccggta cggtcgcgag
     1861 tctgatccat tcttaggttt ttaaattctg cataagcgag ctccggtagg ctcatctttg
     1921 aagtttttgg cggccttttt tcaaaaatgg agactcttat ttcaccggca gtattttttt
     1981 cggcacactc gcaagccttt ttgatagcat ctaaatcact cgtcgagaaa tactttttaa
     2041 tcaggctcat ttattcctca ttaaaatgaa aatcagttaa tcaccagttt nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttgatactgt tcccgctgtc gtggatcggc
     2221 ttggtattca tttttggtag ccatcataat tgcccgtaag ccttgatcta ttccggcgta
     2281 ataatttcct ttgttgaatt ctggtgctat aacattgcgg ataatttgag aactgataag
     2341 atcggtcagg acgccctcca gaccgtaacc gacttcgatc cgcatctggc gatcttgaac
     2401 tgcaattagc aacaaaacgc cattgtccct atcggcagta ccgagtttcc actgatcggc
     2461 gacccgaatc gaatattgtt cgataggttc gccttctaaa ctattaataa tcaaaaccgc
     2521 aatctggttg gaagtttcat tctcaaaggc gatcaggttt tcttcgaggc gatttaactc
     2581 ggaaggagta agaattccgg ctaagtcagt tacatgccgc ttcaaaggtg gtacatccag
     2641 tccgaaagcc ttcaggttga aaagcaatag tccaacgagc gctgggattc gccagttgcg
     2701 gaattttatt ttaagcataa tccgtaatta aaactcgact ttgggggcaa cttcggcgcc
     2761 ttcctgggct tcaaagtaca tttttttctc aaatccaaac attccggcaa aaatattgtt
     2821 cgggaaacgg cggatgcggg tattgtaagt ctgcaccgcc tggttgaagc gttgtctttc
     2881 aactgcaata cggttctcag tgccttccaa ttgcgattgg agtgccaaga aattttcgtt
     2941 ggcttttagg tttggataat tttcagccac ggccattaag cgcgataggg ctgtgctaag
     3001 ctcgccctgt acttgctgga atttggcaaa agcttccgga tcgttcagca cttccggggt
     3061 aacgttcaag ctggtagcgt tagcgcgcgc ctgaatgaca ctttctaagg tttcgcgctc
     3121 gtgagcggca tagcctttga ctgtctcgac taaattggga atcagatcag cccgccgctg
     3181 atattgatta cttacctgag cccaggcttg atttacactt tcttccagcg aaactaagtt
     3241 gttgtaaata ttgattactg cgccgactat tagtagaacg acgacgacca cagcaattaa
     3301 gataacccaa ccttttttca tatttttaac tccttttcaa attactcggt aaatctaaga
     3361 aatttttaca cattcgacaa tcaccaggct aaaaaagaaa tcggaaatta attataaaag
     3421 aaccagattg accttgacgt gttgattaaa gggtagtcgt ccaggtttgg tgatctgact
     3481 attcgaagtt aaattccgcc gttttttaat ggtattttca ttggagttta gatgcaaatc
     3541 ggcattaccg gactgcctta ttccggtaaa tcaaccctgt tcgctacttt attaagtcgc
     3601 aaaagcgaat ccgatccttt tcacaaaaaa gaaaccgaaa aaggcatcgt aaaagtgccg
     3661 gatgtccgac tcgaccgtct aacggaaatt ttcaacccgc gccgtaaagt tccggcaact
     3721 attgaatatc tgaaagtccc cgggctggaa agtcaatcat ctgccgctaa aggcttaccg
     3781 cctcagtttc tcgctaattt gaaaaccgtg gacgtgattc tgctaatcgt cagagctttc
     3841 gataatccgc tttacccaca tccgctgaat cgcattgacc cccagggcga tattaatttt
     3901 gtcaatagcg aatttcttct aaatgacctg gctatcgtcg aaacccgcat cgaacgtctc
     3961 gagaaacaac tcagtaaaat gcataatttg tcctttcaga aagaactgga agtacttttc
     4021 aaatgccgca attttctcga agatgaaaaa ccactgcgtt ctctggcgct tttaccggaa
     4081 gaggacaaaa tcttacgcgg attccaattc ttaactgcta aaccattaat cattgtcctt
     4141 aatatagacg agaaagaaat ccagcaagca gatgaaatca ccgaacgtta cgctaacttg
     4201 aagactgtca acactactta taccgtttta tgtgcggaac tggaaaagga aatttctgaa
     4261 ctcgatccgg ccgagcaacc gctcttttta accgaactgg gaattagcga accagccctc
     4321 aatcgtctga tccgtttatc ttatgaattg ctaggctata tttcattttt taccgtcggt
     4381 gaggacgaat gccgcgcctg gacaatcatc aacggcgaca acgctcagaa agccggacgg
     4441 gctattcatt ccgacatcga acgcggcttc atccgcgccg aagttgtgca ttatactacc
     4501 ttattggaaa acggcaatct ttctacttgt cgagctaagg gtttgattcg ctcggaagga
     4561 cgcgattata tcgtcagaga tggggatgtg atcgaattcc gatttaatgt ttaaagaaat
     4621 ttaccttatt tatccgcgga gagcgccaag aacgcagagt attagtgaaa attggtaaat
     4681 taaatctgta tttaataatc atgttaatct tgtttatcct gttaaaataa atccagtcac
     4741 aacagttttt caaaccagtt ttgcaccggt tccagcggaa cgaagctttc gattaacaat
     4801 tcgggttttc cggcttcgat ggacgaccgc cattccggat aacgcacaac aattaccgat
     4861 tgcatttgat tgaccttggc acagatgagg tcatgtaccg tatcgccgat aataatcact
     4921 ttgttcggcg gatagctctc gctaaataac tcatgaacct ttctcagcgc aaggcgcggc
     4981 agttcattac ggttggcgtt gtcatcgcca aaaacgccaa agtcaaagta gcgccatagg
     5041 ccaaaaggtg cgagcttaat ttgcgcgccg cgcttagcgt tgccggttaa taatcccatc
     5101 cgaaccggta gagtcttgag ataatccagt agttcaatcg cacctggata aattttttta
     5161 tccggagcgg ctggataatc ccagctaaaa atttcaagat agcggtttaa aatttttgtt
     5221 ggcaaaccat catccttaag cccaaggccg agttggttga gagtattagt aataattagg
     5281 ggatcggtca aacccgctaa atggttatct tcaaaggtaa ccggacaacc agtaatctcg
     5341 aaaatagcct gtttgagcgc tttccgagga gtcagcccag acttgataag ggtaccgtca
     5401 atatcaatta gaacgagaag tttattattg gacatcattt aatttaaatc gcactcagtt
     5461 attagaatct acctttaatt tacaatgaaa attaattcga atgttacgat gaattccgac
     5521 cgacgcataa ttttcagatt ccactattaa aaatcaaata accgtgacaa aatctgaata
     5581 tttccggaaa atcgcaagtt ttttcgctca gcttatacct aaaaaataat tggaggtctc
     5641 ggctcaaaat gataatttcc acgccgattt tcctatgatt gagctatcga atgccaaaag
     5701 attttgaact gattattttt gatatggatg ggactcttta tcccgtttcg gcggaaatgg
     5761 attctattta tccgatcgtg gctttggaat gtgccgctaa atcggtcaat cgtcagccag
     5821 cggaaattaa agctgatttt gaacaaaaac gggcggaatt ggcgcgttat ttaaatggca
     5881 tccctaccag cactctgact ttaatgtatt tctatgacgc cgatatgcac gcttttgaaa
     5941 tggaaattac ccgacgcatt ttgactaaga aatatgttca gccggatgca cgcacgattc
     6001 gggtaatcga tgctattgcg caattctatc cgatttttct ctttacaacc aataaccggc
     6061 ttacagctga aaaaattctc gaccagatac ggctatctga atattttccg ccggaacgac
     6121 ggttcacttt cagcgatatt aatgctctgc cgattagcaa gacagaaaaa atcaatttta
     6181 tgaaaccgtc aaccaaagga tttgagcata tcatggaaaa atgtggcgct aatcccaatc
     6241 ggacgcttat ggtcggcgat tccgaaacta tggacatcga gcctgcccga cggctcggga
     6301 tggaaatcta tcacgttacc gaaccggcgt tactctatgc tttgccggaa tggttggaaa
     6361 ttgagcacaa tgataaaaaa atttaggtta ttaaatatat tggcaaaaaa atctctaaaa
     6421 attacttgca tcttagttaa atctatttta ttttttattt gtgttgtgat taattaaaag
     6481 aaaattgaat tcaaatgatt tatcgaggca ttataattaa ttcaaaatcg gcgttctcac
     6541 gcaactatgt taaagttgcc aagatcgcta agaaacccga tttgtaaccc cctaatagaa
     6601 tgaatgtatt tttatacata gggggtccaa gtagtagacc ccctttttgt ttttataaat
     6661 ctaaaaagtg aggaagttat gtttaaagga ttaggaacag cgttgattac gcctttcacg
     6721 gaaaatggag aggttgatta cgatgcgttc cgtaaattaa ttcattatca gattgatggc
     6781 ggagcggact gtctaatagt gctgggaact actggcgaag caccggctat tctgagccat
     6841 gaacgcgaaa agattgtcca gattgctatc gcggaaacca atcatcgcat tccggtaatc
     6901 attgggaccg gctcgaattg tctcgatacc gttttgaagt ataatcgtat ggcagaagaa
     6961 ttgggaagcg atggattatt gatcgttacg ccttattata acaaaggcac tcaagaatca
     7021 atttttcaat attaccgata cattgccgag cgtaccaaat taccgatcat cgtctataat
     7081 gttccgtcgc gcaccggttt caatctcctg ccggacacga tgtttcggat tttcaatgct
     7141 tgtcccaata tcatcggaat taaagaagcc aacgccgatt ttggtcaatt gacgaagtta
     7201 atggcaggac gcccagaagg tatgctggtt tactccggaa atgacgacat tacactgccg
     7261 tttatggcgc tcggagccga aggcgtcatc tctgtcgttg gcaatatcat tccatccgaa
     7321 atgaagcagt tgacagtacg gatggcggct gatgatctgg ctggcgcacg tgaaattcaa
     7381 aatcgctatc tggatttgat caatgccatg tttgtcgaag taaacccgat accggttaaa
     7441 tatgcggcat cgcgcctgaa tctgtgccaa aacgttctgc gattaccgtt aatcccgtta
     7501 tcggagaaaa atgcgcaatt agtagcgagc ttaatgcaag caaaaggatt aatatgaaat
     7561 acggaataat tggctttagc ggccgaatgg gtcacgaaat agatgaactt ttccgtgaaa
     7621 aaggccatga gtgtgttctc ctaattgatg ataagacaga aaaagtcgaa tcggtgccag
     7681 aggtaattct cgacttctca ctgccatccg gcattgaaaa aacaataacc aacgtcagaa
     7741 aatatcaatg tgcgttgatc accggagtta ccggttatag cgcggaacaa ttggaggcaa
     7801 ttcgtgctct tggtcaagaa gtaccggtag tgcagtcttt caatttttct ctcggcatcc
     7861 agctaatgtt gaaaatggta aacctgttaa aagaaccgct caaagactgg gacgtggaaa
     7921 ttgccgaaac tcaccaccgc tttaaaaagg ataaacccag cggtaccgcc aaaatgctcg
     7981 ccgcaactat cggcagagag gtgccaacca gctctttccg cttgggaaac gttcttggtg
     8041 accattcgat cgcctttggc agtttaggag aaatcattac gttgtcccat cgcacgctct
     8101 cgcggcgcgc atttgcggaa ggcgcattgc tatcagccga atttgccatt cgtcagaaat
     8161 caggttattt cacatttact gacgttttgt ctcaggattg atgcgagatc ggctgggttc
     8221 caattagact ttatcaaacc gaaaatattc aattgctaaa ctattttcat agtcgaaaat
     8281 tattttcatg gcgaagaata cgatatatgg aattacagtc agtacaatcg taatgagaaa
     8341 acgtacgtaa gttttcaatg gactaaatca taatgaacaa cgctcaatac aattggatca
     8401 tcattgtaca atggaaaaag taagtgattg actattcaga tttaggcaat taattcattc
     8461 tcgccattga ttatttcaat ttttctgtct ataattccgc cgtatggata aaaataccca
     8521 cgaattattt gaagtcacac tttctgaaac ccgcagtcat gtattagcct ccactaccaa
     8581 cggaattgtg gtcgaaatga tagagcgtct cggtgaaatt gacttcggtc ggattgtcaa
     8641 aatttctgaa acactggtgg acaatttcgg ccagaatgct cgctttaacc gcaatatgat
     8701 caaaaaatat ttcaattatc cggaaaccct acctttcgtg attcgctata atggtgaaat
     8761 cgaaggtttt attatcgggg taccgcttga atatttcgat aaagaaagct gggcgcaatg
     8821 cgacgaaaat ctcggcaaga ataacacgat ttatacttat gcttttatcg tgcgtaaaga
     8881 acatcgggcg ctggggttat ccaaaatgct caagcgggtt taccaaaata ccttgaaacg
     8941 tcgcggtttt ctatacatca caggccacgt aatgcagggg gtgacggcta attttacgcg
     9001 cgattataaa atcgtccggc aattcgaaaa ttggaacaat accggcttta cttttgatta
     9061 ctaccgttcg gcactaaaat aatcttttga tgttttaatt gaaatatttt aaagcctttg
     9121 aataggtttt atttcttttc tgcctgagca acgatagatt tgagaaaatc cagaaactgc
     9181 ccggcaaatt cctcgcgttt gagtgccatc tcgactgttg ttttcaaata atccagcttg
     9241 ttaccgacgt catagcgttt accttcaaat ataaaggcat agaccgactc gcgctgtaat
     9301 aaaagttgga ttgcatccgt cagctgaatt tcgttgtttt tacctggctt aactttttcg
     9361 agcattgagt atatggtagg agttaaaata taccggccgg ctactgctag attggaacgg
     9421 gtttgagtgg gacgtggctt ttcgatcagc tgattgactt tgtaaacacg ctcactgact
     9481 tcgtctccgg aaacaatacc atacatttgg attttatcaa gcggtactct ttcgaccgcg
     9541 atgatagtct ggtgatactg attgtaaata tcaatcagtt gttgagttac cggaatgaca
     9601 gcatcgttaa tagtatcgcc gagcatcact acgaaaggct catcgccggt atgttgtctg
     9661 gcgtaataga ttgcgtctcc aagaccattg agttgcttct gccggatata atgaatatta
     9721 gccatttctc cgatcctgct gatactggta gccaaatcac tctctccttt ttgctcgaga
     9781 atgtgctcca attccgtatt gcgatcgaaa tgatcttcaa tagcacgttt gcctttgccg
     9841 gaaataatta gaatgtcatc aattccagaa tctatggctt cctggacaac gtattgaatt
     9901 gtgggagtat caattatcgg caacatttcc ttgggttgag ctttggtagc tggtaaaaag
     9961 cgcgttccta atccagcggc tggtatgact gcttttttga tcattttatc catttcctcc
    10021 taaataatat taaacaatgt agggtttgat cacccgagca ttaattttct ttagattaac
    10081 gattaagcgg ttgagcatcg catcatcttt ataaatcccg ataatggaac cacctgagcc
    10141 ggcaaattta gcggaagcgc cgcatttcct agcgacatca atcatttcct gattgctttc
    10201 actaatagtc ataattttct tacgattatc gaaattttca ttgatcagcc gattcagagt
    10261 tgcctcatca ccgttctcaa tagccgcttt cccctcctcg gctaactcag caatacggct
    10321 taaacaggcg atgacttccg gatcgccgcg ttcataacgg actttaatat cattcaacga
    10381 ctgacctgat actttactca gcgaagtttt ataagctaga taaagtttgg gtagtttgct
    10441 tttatccaac ggaatataaa ttccgtggtt tttttgctcc ataatttcgc gattgaaatc
    10501 catatagaca cagccttcgt aaacctggat gacgcgatcc tgcaaaccgg cattgatgcc
    10561 cagttcatcc acctctgccg ccagaatcag gctcggtaat aattccttag gaatcgttac
    10621 tttaaaaaat ttcattaacg ctcgcattgt agccgtcaca atcgcactcg aaccacccag
    10681 accaacctga cgcggaatcg aagaattata gcgaatggaa aaattgcggt agtccagctt
    10741 gatattattc tccatacaat aagtgaaaaa tttccgaatt gcggctttga ttaagcgatc
    10801 accaccataa taacccaacg aaccgatccg ttcaaccagg tcataagcgt ttttatagac
    10861 gtttcggtca gaatcggacg attcgatttt taattccggt gtttcgtaga gtaagacatg
    10921 cgccccgaaa ttagcgacga tgattgagat ggtttttccg aaatagccat cggaaggatt
    10981 accaagcaat ccagctctgg cataagcatg agtttcgatt atcattatgt taaaccgatt
    11041 tttatcatat ttagattcac aacgataata tacttag
//
LOCUS       DAOMJU010000070        10991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6101_length_10991_cov_17.790234, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000070 DAOMJU010000000
VERSION     DAOMJU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10991
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6101_length_10991_cov_17.790234"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PLO58_07220"
     CDS             <1..1108
                     /locus_tag="PLO58_07220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HQO74617.1"
                     /translation="ALEAVGGFDQSLKYNALYDIRLKLSEKYKLVHIANKIDGSLYRV
                     VSGGVKHNVFDYLLVSKEAQLEAEVVLTNHLKRIGAYLAPDQGYHLRPDLNEKYDCMA
                     SVIIPVYHRPEFIGNALNSVFSQSVKDLEIIVVVNGGPLDPTVTEVRRYMAGGDRYDA
                     QQPPVRLIVADINNIGLSLNLGLKIARGRYYVQLDSDDRLKSDAIEKIVAVFNSDERI
                     GMVIGSYEVWEKKPSGEFVRMEEIPIVKHEEWTDANGRNNLLRINGAGAPRAIPIKII
                     REMGYFGMNDEPFACNYGEDYDLVLKISEQYRIGRVWQPIYEVVRHTGGTDHSIDQNT
                     IDRNDEAKDYMRLKAVHRRKKINEITNRNLEKSL"
     gene            1111..2358
                     /locus_tag="PLO58_07225"
     CDS             1111..2358
                     /locus_tag="PLO58_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74618.1"
                     /translation="MANCKKDIADDEFLLIGIDGGATKVSGWTVKAENHGTAFALGNF
                     HATKSYTEIPGYIHNFQPVDLNRQIAEWEAGKIVPTTAELVQSSCYIKAASWCIEQIT
                     KRSGTNRVLIGIGMPGIKTRDGRGIAVLKNGPRITNYAVRLENEITFAGIELVQPINQ
                     IGSDAYYCGIGEEYAGEGSFRNVRNSYYLGGGTGAADALKLDGKVIPLDETKGWFVKT
                     WEMKCPDGYSVEKYVSSSGIQSIYGELIGKSPEELTRNGIFPPQIRRRALEGEKAAAI
                     TMQKVAHFLALLFYERITSIYAGWQGIFDFVNPDRPRPQTENNYKKALFDNLVIGQRL
                     GELLCEAQGDEVLWLPFVNELTQLIVNSSALDEVAKEHYCPQKHLNLARIQISPLREA
                     PALGAAIDAYLTWKERKLCLNQM"
     gene            2340..3689
                     /locus_tag="PLO58_07230"
     CDS             2340..3689
                     /locus_tag="PLO58_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HQO74619.1"
                     /translation="MLEPNVIPTEEPILRVGIFLPIDKCQRIALVIPQSPVYELIPES
                     GPRQILKGGEQIEFILFENQIGIQINGLTPNAQLSWKIIPRGGEIQIANKAGIKVKNV
                     VAGRGFHWEKYIDVYLPGSIEISRMNDNLIMINVLPLEQYLMCVATSEMGAACPLSVI
                     KSQTIIARSWLLANVEMKHRNLGMDVCNDDCCQRYQGTTFLSEHSIKGALETTGQVLI
                     YGEKICDTRYHKNCGGVTESFENVWNGSAVPYLDSVLDIPPDCPHSALPLDTEAKVRN
                     WIENPPLAFCCPSIVPEKELKKYLGNVDKEGHYFRWHFRMKQREITALFNLKLNLDAA
                     AIKSIEPLKRGKSGRLTSLAINYRDKANVDKQIVINDQYAIREAFHELFLYSSAFVVD
                     VEEGSENIPAAFVLKGAGWGHGVGYCQIGALGMALKGYSTLEILEHYYPGVKLHQAY"
     gene            3709..5382
                     /locus_tag="PLO58_07235"
     CDS             3709..5382
                     /locus_tag="PLO58_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO74620.1"
                     /translation="MASEPATVKNRSPWLWVPSLYFAEGIPYVIAMTVSVIMYKTMGV
                     SNAALAFWTSVLYLPWVIKPIWSPFVDVISTKRNWVLITQFILGCCFIGVALVVRLPL
                     WFALTLIVLWIIALASATHDIAADGFYMIGLDAHQQTWFVGIRSTFYRMAMIVGQGLI
                     VMLTGYIMSKTGLKPVSITAMAVPPNEIMQVLDENNLPAIKASEQPNIVVIPAEVKVP
                     IYNPAEPGSDSANVFICLSAPPKKEETVVVNFGRKKGSKDIWLTSAGRLEFTAENWNK
                     PVKVTLKVDHRLREPVVSQFEATAGDIPFSWAVSFGILGVFFLLICLYHKVMLPTGLE
                     TVRTKSQELKASWFMVFSSFFRKKGIVSALLFLLFYRLAESQLVKMASPFLLDSQEAG
                     GLALTTTQVGFVYGTVGIICLTIGGILGGIVAARNGLKRWVLWMALAINIPDLVYVYL
                     SYVQPDNFMIVTICVAIEQFGYGFGFTGYMLFMLYLAEGEYKTSHYAIATAFMALGMM
                     LPGMISGKLQDLMGYRTFFVYIMFCTIPSFLVASLVKIDPDFGKKKIEN"
     gene            5390..6709
                     /locus_tag="PLO58_07240"
     CDS             5390..6709
                     /locus_tag="PLO58_07240"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HQO74621.1"
                     /translation="MKIIWDKRRSFFGELILLLLVFSEINGQTAKLRHSTVITLPVDE
                     ETQDFRIGLDLLIDDNFHALDNYKFMVIADRNSFAQGNKYIFDIVNIRLGTRCRSCVQ
                     VLDDRSLLTGSGFQLSQSQLYGITKYYILNSERDRLSASMLNGAEMIVLDLQDFGLRE
                     SEALRILIEALKFSAKQKIPIIVLDRPNPLRGVEIGGPVSIQSKFAADFSLPARHGMT
                     LGELALLMNEENWLNAGDKSQLTVIKMANYYRDMPWQQTGLKWSFADESVQDEETAVL
                     YAGLYFFQFTNISVGTGTPHPFHVVGAPWMSANELVNALAKYDLAGLEISQVKFIPRR
                     ELNATELPLYMDTECLGINLKVSDLNKFDAYKFSATLISVISRLYPKHFKWIKTTEAD
                     AYFGNADFRTWVNIGADLNPMLADWEAKASRFQKLRLKYCLYPVQKN"
     gene            6885..>7267
                     /locus_tag="PLO58_07245"
     CDS             6885..>7267
                     /locus_tag="PLO58_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74622.1"
                     /translation="MKIRRVLLCIVLLVSGTVLAGGLVTNTNQSAAWVRTLNRNASTD
                     VDAVYYNPAGLTKLFEDGLFLDVSNQTIWQTKTVTNDTLTLNNHEFVGKVFAPVFPSA
                     YVAYKMNKLVLSGGFMPIGGGGSAK"
     assembly_gap    7268..7367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7368..7907
                     /locus_tag="PLO58_07250"
     CDS             <7368..7907
                     /locus_tag="PLO58_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQO74623.1"
                     /translation="ISPNENLNIGIRYETITKMELENDTKKDDTGLFPDSAKYGADIP
                     SQLAVGVSYNMGKIKLMMDFNYYNNGAVDWDGDEKNFDNTTEFGLGAEYALTEKLKVS
                     LGGQYSMMGAKPESQSDMDFNLDAFTIGGGVEYQISPKVKLNVGALDAFYIEAENASG
                     IEKYNQTNYLVAFGVQVKL"
     gene            7979..>8973
                     /locus_tag="PLO58_07255"
     CDS             7979..>8973
                     /locus_tag="PLO58_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQO74624.1"
                     /translation="MKRDLLLIVALISLISIEGTMAKERTGWGWGGVPAINYNADEGF
                     GYGMIFDIFNYAKGGYSPYYFKIDNQIFFTTGGKQDHTIFFDSPYILGNGFRISVRTR
                     YKKEKFQPYYGLGNDSEFDPGYIEVDEDGNSLDSLHGKHYYTFNSEQIIFYTNILKAL
                     KYREDGRPLISVLGGLGFYQLDCGANDNGGIPTKFEEDLNAGILKKHDTDKSFNDYLK
                     FGLVYDSRDNEPAPNRGVWTDIVGEWYTSLIGSDNDFLRLTLTDRRYFTLRKNLVYAN
                     RIIVENIFGEAPLLLYYPLGGSFRIDEGVGGYRSIRGAYKNRYVGSAKFIMNMELR"
     assembly_gap    8974..9073
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9074..10834
                     /locus_tag="PLO58_07260"
     CDS             <9074..10834
                     /locus_tag="PLO58_07260"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO74625.1"
                     /translation="KDKQVFAWAMYDWANSAFATTVMAGFFPVFFKGYWSAGTDVNVS
                     TAMLGYANSIGSLIVALISPILGAIADRSSSKKRFLIFFAYLGVLTTTCLFMVDYGQW
                     LWAVIFYVAGSLGFAGANTFYDSLLPEVAPPHKIDYVSAKGYALGYLGGGLLFLINVL
                     WYLSPATFGFPVEQQANVYQALTQNATVIQIEPGKDFSIPKKHSIGKAVVTSEIKLPI
                     TIVDYHSDDSVWISVPVPPEVDQDLIVSAISFGQFKSGEIVAKNHSKNEYLIGNLNQK
                     LSPGDSAVIIIRNPITYQSFRDQRIEGVEGLSNHCGRITITTDYLTPAQENLPIRVSF
                     LSVGLWWGIFTIPLILYVKEKRREPRPNRRSTYIRQGFKQLSQTFQQVRHLKVVMIFL
                     VAYWLYIDGVDTIIRMAVDYGLSIGFPSSSLIVALLIVQFVGFPAALAFGKLAEKWNP
                     KKVIFATIAVYLGVTAYSVLMKDVKEFYILAIVIGCVQGGIQALSRSFYLRLIPKEQS
                     AEFYGFFNMIGKFSVIFGPFLIATVGLLARRAGFSADLASRIGIGSIGILFLVGAILL
                     CFVNEEKGRAEAEKLRILNK"
BASE COUNT         2982 a         1993 c         2633 g         3183 t
ORIGIN      
        1 ggctttggaa gcagttggcg gttttgatca gtcattgaaa tacaacgcct tatatgacat
       61 tcgtctaaag ttatcggaaa aatataaatt ggttcacatt gccaataaaa tagacggctc
      121 gctttatcga gtggtttccg gcggagttaa acataatgtt tttgattatt tgttggtgag
      181 caaagaagcc cagctggaag ccgaagtagt tttaactaat catttgaaga gaatcggagc
      241 ttatctcgcg cctgatcaag gttatcacct gcggccggat ctaaacgaaa agtacgattg
      301 catggcttcg gtaataattc cggtttatca tcggccggaa tttatcggta atgcgctgaa
      361 ttcggttttc agccagagcg tcaaagacct tgaaattatc gtcgttgtaa atggagggcc
      421 tcttgatccg acggttactg aagtccggcg ctatatggcg ggtggagacc gttatgatgc
      481 tcagcaaccg ccggttagac tgatcgtagc ggatattaac aatattggac tatcgttgaa
      541 tcttggttta aaaattgccc gcggtcgcta ctacgtccag cttgattctg atgatcgttt
      601 aaaatcagat gcgattgaga aaattgtagc tgtcttcaat tctgatgaaa gaatcggaat
      661 ggttatcggt tcgtacgaag tctgggaaaa gaaaccaagt ggtgaatttg tccgaatgga
      721 agaaattccg attgtcaaac acgaagagtg gacggatgcc aacggacgta ataatctctt
      781 gcgtatcaat ggtgccggag ctccacgggc gattccgata aaaattatcc gggaaatggg
      841 ttatttcggg atgaacgacg aaccgtttgc ttgcaattat ggcgaagatt atgacttggt
      901 gttaaaaatc agtgagcaat atcgcatcgg acgagtctgg caaccgattt atgaagtcgt
      961 gcggcatacc ggtggaactg atcatagcat tgaccaaaat actattgatc gtaacgacga
     1021 agccaaagac tatatgcgtt tgaaagccgt tcaccgacgg aaaaaaataa atgaaatcac
     1081 taatcgtaat ttggaaaagt cactttaaaa ttggctaatt gtaagaaaga catagcagat
     1141 gatgaatttt tgttaattgg aattgacgga ggggcaacca aagtcagcgg ttggacagtg
     1201 aaagccgaaa atcacggaac cgcttttgca cttggcaatt tccacgcgac aaagtcctat
     1261 actgaaattc ccggctatat ccataatttc cagccggttg acttaaatcg gcaaattgcc
     1321 gagtgggaag caggaaaaat cgtgccgacg accgcagaac tcgttcaatc gagttgttat
     1381 atcaaagccg cttcctggtg tattgaacaa attaccaaac ggagcggcac caatcgggtg
     1441 cttattggaa ttggtatgcc aggtatcaaa accagagacg gccgcggcat cgccgtcctg
     1501 aaaaatggcc cgcgcattac caattatgcg gttcgactcg aaaatgaaat aacttttgct
     1561 ggaatagagc tagttcaacc gatcaatcag atcggcagtg acgcttatta ttgtggaatc
     1621 ggcgaagaat atgccggtga aggttcgttt cggaatgttc gcaattctta ttaccttggt
     1681 gggggaaccg gtgcggcgga tgctctgaaa ctcgacggga aagtcattcc gcttgacgaa
     1741 accaagggtt ggtttgttaa aacctgggaa atgaagtgtc cggatggcta ttccgtggaa
     1801 aaatacgttt cttcgagcgg aatacaatcg atttatggcg agctaatcgg aaagtcgcca
     1861 gaggaactaa cccggaacgg tatctttccg ccccaaattc gccgacgggc tttggaaggc
     1921 gagaaagcgg cggctattac gatgcaaaaa gttgctcatt ttctggcact tcttttctac
     1981 gaacgaatta cctcgatata tgccggttgg cagggaatat ttgactttgt caatcccgat
     2041 cgtccacgac cccaaactga aaataattat aaaaaggcgt tatttgacaa tctggtcatc
     2101 ggacagcgat tgggtgaatt attgtgcgaa gcccaaggtg atgaggtgct ctggttgccg
     2161 tttgtcaatg aattgactca attgattgtc aattcttctg ccctcgacga ggtagccaaa
     2221 gaacattatt gtccgcaaaa gcatttaaac ctggcgcgaa ttcaaatttc ccctctgcga
     2281 gaagcgccag cgctgggtgc ggctattgac gcttatttaa cctggaagga gagaaagtta
     2341 tgcttgaacc aaatgtgatt ccgaccgaag aaccaatcct gcgggtagga attttcctgc
     2401 caattgataa atgtcaaagg attgcgctcg tgattccaca atctccagtt tatgaattaa
     2461 ttccggagag cggcccgcgg cagattttga aaggcggcga acagattgaa tttattttat
     2521 ttgaaaacca aatcgggatt caaattaatg gattaacacc caacgcccaa ctgagttgga
     2581 aaatcattcc gagaggcggt gaaattcaga tagcgaacaa agccgggatt aaagtcaaga
     2641 atgtggttgc cggacgtggt tttcattggg aaaaatatat tgatgtttat ctgcccggct
     2701 caatcgaaat aagtcggatg aatgacaatc taattatgat aaatgtattg ccgcttgagc
     2761 aatatttaat gtgtgtggca acttccgaga tgggagcggc ttgtccatta tccgttatca
     2821 aatcgcagac aatcatcgcc cgcagttggt tgctggcgaa cgtggaaatg aaacaccgca
     2881 atcttggaat ggacgtttgc aatgacgatt gctgtcagcg ttatcagggg acgacatttc
     2941 tttcggagca ttcgattaaa ggtgcgcttg aaaccaccgg tcaggtctta atatacggtg
     3001 aaaaaatctg tgatacccgc tatcacaaga actgcggcgg agttacagaa tcttttgaaa
     3061 atgtgtggaa tggttcagct gtgccgtatt tggattcagt gcttgatatt ccgcctgatt
     3121 gcccacattc ggctttgcca ctcgatacag aggcgaaagt ccgcaattgg atcgaaaacc
     3181 cgccgttggc tttttgttgc ccatccatcg taccggaaaa agagttgaaa aagtatcttg
     3241 gcaatgttga taaagaaggt cattattttc gctggcattt tcggatgaag cagagagaga
     3301 ttacggcgct attcaatctg aaattgaatc tggacgcggc cgccatcaag agcatcgaac
     3361 cattaaaacg gggcaagtcc ggacgactga ccagtctggc gattaactat cgggataaag
     3421 ccaatgttga caagcaaatc gtgattaatg atcaatatgc cattcgtgaa gcatttcatg
     3481 aacttttcct ttacagttcg gcttttgtag ttgatgtgga ggaaggatct gaaaatatcc
     3541 cggcggcttt tgttctgaaa ggagctgggt ggggacacgg ggttggttat tgtcagattg
     3601 gggctctcgg gatggctctg aaaggctatt caaccttgga aatattagaa cattattatc
     3661 cgggtgttaa actccaccaa gcatattgaa ttactcagga gaaaagtaat ggcaagtgaa
     3721 ccggcgactg tgaaaaatcg ttcaccctgg ctttgggtac caagccttta ttttgccgaa
     3781 ggcattccct acgtcatagc gatgacggtt tcggtaatta tgtacaaaac gatgggagtt
     3841 tctaatgcgg ctttggcgtt ttggaccagt gttttgtatt tgccctgggt aatcaaacca
     3901 atctggagcc catttgtaga tgtaatttcc accaaacgca attgggtatt aatcactcaa
     3961 tttatactcg gatgttgttt tatcggggta gcgctagtcg tccgattacc gctctggttc
     4021 gctttgacgt taatcgtttt atggataatt gctttagcct cggcgactca cgatatcgcc
     4081 gctgatggat tttatatgat tggcttggat gcgcaccagc agacctggtt cgtcggtatt
     4141 cgcagtacgt tttaccgaat ggcaatgatc gtcgggcaag gcttaatcgt gatgctaact
     4201 ggttatatta tgagtaaaac cgggctaaag cctgttagta ttacggctat ggctgttccg
     4261 ccgaatgaaa ttatgcaagt gctggatgaa aataatttgc cagcgatcaa ggcatctgag
     4321 caaccgaata tagtcgttat tccggcggaa gtaaaagtgc cgatctataa tccagccgag
     4381 ccaggtagcg attccgctaa tgtttttatt tgcctgtcag cgccgcccaa aaaagaggaa
     4441 accgttgtag taaatttcgg ccgcaagaaa ggtagtaagg acatctggtt gacatcagct
     4501 ggtcgcctgg aatttacggc tgagaattgg aataagccgg tcaaggtgac tttgaaagtt
     4561 gatcaccgtc tgcgagaacc ggtagtgtcg caatttgaag ctaccgccgg tgatattcct
     4621 tttagctggg cggtatcgtt cggaatttta ggcgtatttt tcctgctaat ttgtttgtat
     4681 cacaaagtaa tgttgcctac cggattggag acagtcagaa caaaaagcca agaactcaaa
     4741 gccagctggt tcatggtatt ttccagtttt ttccggaaaa aaggcatcgt ttcggcttta
     4801 ttatttctgt tgttttatcg cctggcagaa tcgcaattgg taaaaatggc ttcgcctttt
     4861 ctactggata gccaagaggc tggcggatta gcattgacaa cgacgcaggt tggatttgtg
     4921 tatggaaccg tcggaataat ttgtctgaca atcggaggca ttctcggtgg aatagttgcc
     4981 gctcgcaacg ggctgaaaag atgggtgtta tggatggcgc tggctattaa tattcctgat
     5041 ttagtctatg tttacttatc gtatgttcag ccggataatt ttatgatagt tacaatttgt
     5101 gttgccatcg aacaattcgg ttacggtttc ggttttaccg gctatatgct ctttatgttg
     5161 tatttggcgg aaggtgaata taagacgtcg cattatgcga ttgcaacggc ttttatggcg
     5221 ctgggaatga tgcttccggg aatgatcagc ggcaaattgc aggatttgat gggatataga
     5281 actttctttg tctatataat gttttgtacg attcccagtt ttttagtcgc cagtctggta
     5341 aaaattgacc cggattttgg aaagaaaaag atcgagaatt agcaggctta tgaaaataat
     5401 ttgggataaa cgtcggtcat ttttcggcga attaatatta ttacttttag tctttagcga
     5461 aataaacggt caaactgcca aattacggca ttctacggta attactttgc cggttgatga
     5521 agaaacgcag gactttagaa tcggcttgga tttgttaatt gatgataatt ttcacgctct
     5581 cgataattat aaatttatgg tcattgccga tcgcaatagt tttgctcagg gaaataagta
     5641 tatttttgac attgtcaata taagattggg aacgcgctgt cggtcgtgtg tacaggtttt
     5701 ggatgaccgg tcgttactga ccggctcagg attccaatta agtcagagtc agctttatgg
     5761 aattaccaaa tattatattt taaatagcga gagagaccga cttagtgcaa gtatgttaaa
     5821 tggcgccgag atgattgtgc tcgatttgca ggatttcgga ttgcgggagt cagaggcgct
     5881 caggattttg attgaggctt taaagttcag cgctaagcaa aaaattccta tcatcgtgct
     5941 tgatagaccc aatcctttac gcggagttga aataggtggt ccggtgtcaa ttcagagtaa
     6001 gtttgcggct gacttttctt tacctgctcg acacggtatg actttgggcg aattagcttt
     6061 gctaatgaat gaggaaaact ggcttaatgc cggagataaa tctcaactta ccgtaattaa
     6121 aatggcaaat tattatcgcg atatgccctg gcaacaaacc ggactgaaat ggtcatttgc
     6181 ggatgaatcg gtgcaagatg aagagactgc tgttctctat gccggattgt atttttttca
     6241 gtttactaat attagtgtgg ggacaggaac gcctcaccct tttcacgtcg tcggagcgcc
     6301 ttggatgtcg gctaatgaat tagtaaacgc tctggcgaaa tatgatcttg cgggattgga
     6361 aataagccag gtcaagttta ttccgcgccg tgaacttaat gcgactgagt tgcctttata
     6421 tatggataca gaatgtttgg gaataaacct aaaagttagt gaccttaaca aatttgatgc
     6481 ttataaattt agcgcgactt taatttcagt gatttcaagg ctttacccaa aacattttaa
     6541 atggataaaa accacagagg ctgatgctta tttcggcaat gctgattttc gaacctgggt
     6601 caatattggc gctgacttga atccgatgtt agctgactgg gaggcgaaag ccagccgatt
     6661 tcaaaaatta cgattgaaat attgcctgta tccggttcaa aaaaattaat tttgttttcc
     6721 ttttcttttc gatatattta tactgaggtt agtggttaat tatagattga gtggttattt
     6781 taaataccgg taaagcagga ctgtagggta gccgaagggg aaaaaatcaa aacaaaaatt
     6841 cttcatcata actctaataa cgagtaaaaa taaaggagaa aaacatgaaa atcagacgtg
     6901 ttttgttatg tatagttcta ctggtgagcg gaactgtttt agctggtggt ttggttacga
     6961 acactaacca aagcgcggct tgggtgcgga ctttgaaccg taacgcttcc accgatgtgg
     7021 atgcggttta ctacaatccg gctggattga ctaaattatt cgaggacggt ttgtttctgg
     7081 atgtcagcaa tcagacgatc tggcaaacca aaacggtgac taatgacacc ctgacgttga
     7141 acaatcatga gttcgtcggc aaagtatttg cgccggtttt tccctcggct tacgttgctt
     7201 ataaaatgaa taaacttgta ctttccggag gctttatgcc gatcgggggc ggcggcagtg
     7261 ccaaattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatc agcccgaatg
     7381 aaaacttgaa tatcggtatt cgttatgaga ccatcactaa gatggaactg gaaaatgata
     7441 cgaagaaaga cgataccggt ctcttccctg atagcgctaa atatggagcc gatatacctt
     7501 ctcagctagc cgttggcgta tcttataata tgggaaagat taaactaatg atggatttta
     7561 attattacaa taacggcgcc gttgactggg atggcgatga gaaaaacttc gacaatacga
     7621 cggaattcgg gttgggtgct gaatatgctt taactgaaaa attgaaagtc agtctcggtg
     7681 gacaatatag tatgatgggg gcaaaaccgg aatcgcagag tgatatggac ttcaacctcg
     7741 acgcatttac aataggcggt ggcgttgagt atcaaatttc accgaaagta aagctcaacg
     7801 tcggtgcgct ggatgccttc tatattgagg ctgagaatgc cagcggaatt gaaaagtaca
     7861 atcagactaa ttatctcgta gctttcggcg ttcaggtaaa gctgtaaaat tatgccagga
     7921 ttaattgata aataggagaa gtcgttacgg cttctcctat ttatattttg aggttaaaat
     7981 gaagagagac ttattattaa tagtagcact aatatcgcta atttccatcg aagggacaat
     8041 ggctaaggag cgcaccggtt ggggttgggg tggcgttccg gctatcaatt acaacgccga
     8101 tgagggattt ggttacggaa tgatctttga tattttcaat tatgccaaag gtggctattc
     8161 gccttactat tttaaaattg ataaccaaat tttcttcacg accggcggga aacaagacca
     8221 caccatcttt ttcgattcac cctatatttt gggtaatggt ttcagaataa gcgtccgaac
     8281 ccgttataaa aaagaaaaat tccagcccta ttatggattg ggcaacgatt cggaattcga
     8341 tcccggttat attgaagttg atgaggatgg caatagtctt gattccttgc atggcaagca
     8401 ttattatact tttaattccg agcaaatcat tttttatact aatattttga aagctttgaa
     8461 atatcgggag gatgggcggc cgctcatttc agtattgggt ggactgggct tttatcagct
     8521 agattgtggc gcaaatgata acggcggaat tcccaccaaa tttgaagaag accttaacgc
     8581 tggaatacta aaaaaacatg atactgataa gtcgtttaac gattacctca aattcgggtt
     8641 ggtttacgat tcgcgggata atgaaccggc gccgaatcgc ggtgtctgga cggatattgt
     8701 gggcgaatgg tacaccagtt taattggtag tgataatgat tttttaagat tgacgctaac
     8761 tgatcgccgt tattttacac ttcgtaagaa tctggtatat gcgaatcgaa ttatagttga
     8821 gaatatattt ggcgaggcgc cacttctgtt gtattatcca ttaggtggtt cattccgcat
     8881 tgatgaaggc gttggtggtt atcgttcgat tcgcggcgct tataaaaacc gctatgttgg
     8941 ctcagccaaa tttattatga atatggaatt gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnaaagaca agcaagtttt tgcctgggca atgtatgatt gggctaattc
     9121 ggcttttgcc acgacggtaa tggctgggtt cttcccggtg tttttcaaag gttattggag
     9181 cgccggtacg gatgtaaatg tcagtaccgc aatgttagga tacgctaact cgattggttc
     9241 gctaattgta gctctaatat cgccaattct gggcgcaatt gccgatcgga gttcatcgaa
     9301 gaaacgcttt ttgatatttt tcgcttattt gggtgttctg acgacgacct gtctttttat
     9361 ggttgactat gggcaatggt tgtgggcggt tattttctat gtagctgggt cgctgggttt
     9421 tgccggtgcg aatacctttt acgatagttt attgccggag gttgccccgc ctcataaaat
     9481 tgattatgtg tctgccaaag gctatgcgct gggttatctg ggaggtggat tattatttct
     9541 cattaatgtt ttgtggtatc ttagtccggc gacttttgga tttccggttg agcaacaggc
     9601 aaatgtctat caagctctca ctcaaaatgc tacggttatc cagatcgaac caggtaagga
     9661 tttttcgatt cccaagaagc attccatcgg taaagcggtt gtcaccagtg aaattaaatt
     9721 acccatcacg atagttgatt atcattctga tgattcagtc tggatcagtg tgcctgttcc
     9781 acctgaagtt gatcaggact tgatagtgtc tgcgattagt ttcggtcaat ttaaatcggg
     9841 tgagatcgtc gctaaaaatc atagtaagaa tgaatatttg attggcaatc tgaaccaaaa
     9901 attgagtccg ggagattccg ctgtaattat cattagaaat ccgattactt atcaatcatt
     9961 tcgcgatcag cggattgagg gagtggaggg actttccaat cactgtgggc ggattacgat
    10021 tacaaccgat tatcttaccc cagcccaaga aaatctgccg attcgggtga gttttttatc
    10081 ggtagggctc tggtggggaa ttttcactat tccgctaatt ctttatgtca aagaaaaacg
    10141 ccgggagccg agaccgaata gacgttctac gtacatccgt cagggattca aacaactgag
    10201 ccaaaccttc cagcaagtgc gccatctcaa agttgtaatg atttttttag tagcctactg
    10261 gttatatatt gacggtgttg atacgattat tcgtatggcg gtagattatg gattgtcaat
    10321 tggatttccg tcgagttcat taattgtggc tttgttgatt gttcagtttg ttggttttcc
    10381 ggcggcgctg gcgtttggta aattagctga aaaatggaat cccaagaaag taatttttgc
    10441 gacaattgcc gtctatctgg gagtaacggc ttatagcgtt ttaatgaaag atgtgaaaga
    10501 gttttatatt ctggcaattg taatcggatg tgttcagggc ggtattcagg ctcttagtcg
    10561 ttcgttttat ttacggttaa tacccaaaga acagagcgcc gaattctatg gttttttcaa
    10621 tatgattggt aagttctcgg taatttttgg gccgttttta atcgcgactg tgggtttatt
    10681 ggctcgccgc gccggatttt cggctgattt ggcttcacgg attggaattg gttcgattgg
    10741 gatattattt ctggttgggg caattttact ctgtttcgtc aatgaagaaa aaggccgagc
    10801 agaagcggag aaattgcgga tactgaataa atgatttttc agcagtttaa aataattggt
    10861 gtcccggata tcaaagaata tcaaaaagcg ttccctcgat aaattcactg tgacataaac
    10921 cgattaactt acttttaaat taattacgtt attaataaaa ttggacgcca tgtcctgata
    10981 ctatataaaa a
//
LOCUS       DAOMJU010000071        10931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6146_length_10931_cov_18.543398, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000071 DAOMJU010000000
VERSION     DAOMJU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6146_length_10931_cov_18.543398"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..567)
                     /gene="folE"
                     /locus_tag="PLO58_07265"
     CDS             complement(19..567)
                     /gene="folE"
                     /locus_tag="PLO58_07265"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HQO74626.1"
                     /translation="METEEIIRNLLINIGENPNRPELLKTPARVAKFWQHVSQGYKVD
                     VLEMLKESLVYEDYNQMIIVKDIDFFSLCEHHLVPFFGKAHVAYIPNGKIIGFSKIPR
                     VVTAFSQRLQIQERLTQQIADALNEALEPKGVGVIIEARHLCMQMRGVEKQNSNITTS
                     VMLGELHDEPQARAEFLKLISR"
     gene            complement(650..1141)
                     /locus_tag="PLO58_07270"
     CDS             complement(650..1141)
                     /locus_tag="PLO58_07270"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HQO74627.1"
                     /translation="MNNLSQVSDSIFNNIRSILEDARSRAYRSVNFIMVEVYWNIGRI
                     IVDEEQKGKRRAEFGKQLIADLSRKLTVAYGRGFDPSNLWYMRKFYLLFPILDAVRRE
                     LSDSLANGDSKSVIRLGNSIRPELTWTHYRLLLRIENPQARSFYMNESANNGWSTREL
                     DQW"
     gene            complement(1138..1776)
                     /gene="nth"
                     /locus_tag="PLO58_07275"
     CDS             complement(1138..1776)
                     /gene="nth"
                     /locus_tag="PLO58_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQO74628.1"
                     /translation="MTNKSEIAPEIFSRLSAAYPDARCALNHTNPRELLIATILSAQC
                     TDKRVNMVTPALFQKYPTAQAFADADLEELKNDIRSTGFYNNKAKAIKSAMKSVVEKF
                     NGEVPGTMEELLQLEGVGRKTANVVLGDAFGVPAIVVDTHVKRLANLLGLTKNSNPEK
                     IEQDLMRQFPQEQWTKLGHLLIEHGRQVCIARRPQCDKCVLNDICPSARITK"
     gene            complement(1842..1982)
                     /locus_tag="PLO58_07280"
     CDS             complement(1842..1982)
                     /locus_tag="PLO58_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74629.1"
                     /translation="MRSDKIIYSINIADVQTVAEQELDRKLTQEELKFIADEALSTDY
                     FG"
     gene            complement(1972..3018)
                     /locus_tag="PLO58_07285"
     CDS             complement(1972..3018)
                     /locus_tag="PLO58_07285"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HQO74630.1"
                     /translation="MTNAKIEKIAFEVIKTLKSRFDTFPENEMDNRNAPFHEAFLNAF
                     SVKLEGKVKSVPFLITLSSWLHGLSTTLGQSFFERVSHILSNGEKREFRDLIISSRQE
                     TVISDIITELKNGQRQPDVSSEDNILKYQGEILDRHIPNFTADVFYETDNEIIAIELK
                     TVKPNSGVFKVEKEKILQAKTGLRNKYLGKKVSYYLGFPFDPTSKQPTKSNKERYFGY
                     SIDFRKYFHPQEILLADELWNFLSGETGTMELILSIINSIAKPDFMDKFDIINQPISQ
                     TATKSKAILHDWFLFTELQIIQNFPHLNDLSIKDKTLKRILAQCPFSPDGKINTNRLD
                     IIRPFLEGGEQNAK"
     gene            complement(3015..4625)
                     /locus_tag="PLO58_07290"
     CDS             complement(3015..4625)
                     /locus_tag="PLO58_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQO74631.1"
                     /translation="MGEMETLFKNQNNILSLKDASRWASDYLNKKVTESNITYLINYG
                     KINKTSNNGSIYVDISELKNYYDSFWGKREINWKNQLGEDLNWHLSFDFLKEADTTKH
                     VHRLHPYKGKFIPQLVEYFLDDHIDEFKTEVFFNKGDIILDPFCGSGTTLVQANELGM
                     HAIGVDVSAFNALISNVKVKKYDLFSLQAEITKITRSLRVFNSKRNILQFDVELTEKL
                     NEFNNRYFPSPEYKQKVRRKEINEDNYGKTKSDEFLPIYWDLVKKYGVKLKQDTENNF
                     IEKWYLQPIREEMDFVFEQIKLIQDSEIKKIISVIFSRTIRSCRATTHADLVTLIEPV
                     ATPYYCAKHGKICKPLFTILNWWERYSKDTLVRLATFNKLRTNTFQYCLVGDSRNIDI
                     IEKLQSKNKELSDLVQRKKIDGIFSSPPYVGLINYHEQHAYAYDLFGFERNDELEIGP
                     LFKGKGIEARESYVQGITDVLINCKRYLKPNYSIFLVANDKYNLYPMIAEKSGMKIVN
                     CYKRPVLNRSEKDKGAYSEIIFHLKDVQ"
     gene            complement(4700..5725)
                     /locus_tag="PLO58_07295"
     CDS             complement(4700..5725)
                     /locus_tag="PLO58_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQO74632.1"
                     /translation="MFEKYYNQIIQGDCIELFKNIPDNSVDVTFADPPFNLGKKYNGY
                     RDNKEVQAYLDWCKRWIFEMVRITKPAGSIFVHNIPKWLTYYASFLNEIAYFKHWIAW
                     DAPTAPMGQSLQPSHYGILYYAKDLKKNKFFEIRYPHKRCRKCGYLLKDYGGKKAGLH
                     PFGPLVSDVWTDIHRLKHNKYRDEHPCQLPVHLLERIILMSSDEGDIVLDPFVGTGTT
                     AIAAKRLGRKYIGFDIDENYITITQKKLVQEELCSKINGIWLSFFLGEPITIRDKDWV
                     ELTKYFVIPDNIKEIEYSKIKLHPNIFIPETVIKSDLSLFENTESEEEVYKHDLCKVC
                     QSQNIGK"
     gene            complement(5718..6707)
                     /locus_tag="PLO58_07300"
     CDS             complement(5718..6707)
                     /locus_tag="PLO58_07300"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009413428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HQO74633.1"
                     /translation="MKTIDEKYRTVIENNTYYYFDEEFESAYESHIFSVKESIIYLRN
                     EVQNNGCKKEVFENFLRNKKELGLKALLAITGFSNESLLRLITIIRIVDDKELNDFTF
                     MNYWINNKRTTKDNEISEWNTSKIEQLVRENEYFRKCLVNIFYEGSSIDFFKRTLPPF
                     EFQKLSLEKLNFDINAIIDTLARYKEKGSRAAKGKNNPEILIERILKKRKIPFEKGDL
                     SELIEYAPEQKRTMDFIIPNKQSPKIIIESTFLVTTSSGQGDKSKTEISIDSLIKEHY
                     PDARFLGFVDGIGWYVRKRDLKRMVGAYEDVFTFHQEELNRFEKLLCEVMKNV"
     gene            complement(6710..>7339)
                     /locus_tag="PLO58_07305"
     CDS             complement(6710..>7339)
                     /locus_tag="PLO58_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQO74634.1"
                     /translation="AIYNLALLAYLDSMGYSKRVIKSDHRQLFEKVLTRYENTVRNFI
                     NNPYFKKVQLGKLNVLSDSDALDLKIVDNSIDGVITSPPYSFAIDYAKNDEAQLEYLG
                     YNIEKIKGKMIGLIGKNKDERLNNYFRDMKKVCSEIYRVLKNDKYFVMIIGSNTNQTG
                     GIRLEKTIIQNCEEIGLKLVKSILKPIKGMRNTMKDEYLLFFQKNPDRN"
     assembly_gap    7340..7439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7440..7765)
                     /locus_tag="PLO58_07310"
     CDS             complement(<7440..7765)
                     /locus_tag="PLO58_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74635.1"
                     /translation="MKVLGNYSFYELPACIETLFDKNVTLLPSVTELFELELAFLEYK
                     SLPKDQIIERSAYIQSIDGKFTTHYLMYNQTCEGLIENRSNVTKNYFENGTFSTGYAT
                     HGLFPYR"
     gene            complement(7902..8828)
                     /locus_tag="PLO58_07315"
     CDS             complement(7902..8828)
                     /locus_tag="PLO58_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HQO74636.1"
                     /translation="MIPFLLDERYYRYGRYLRERFQTRVYRITLDAGFTCPTRDGTKG
                     TSGCLYCNNESFAPKRAEELPSLSDQIQKGIASARRFHQADKFLAYFQPFTNTYAPVA
                     KLERLYHEVLAFSEIVGICIGTRPDCVSEEILQLLEHLNHETYISIEFGIESVYDKTL
                     DWAQRGHDFAQTRWAIQAAKAHGLHVAGHLILGFPTETRAEILATPGVLNDLGIDALK
                     IHHLHVVKNTPLAKFYAENPFSLFTEKEWITLVADFLERLHPEIVIQRLCGDAKPGTL
                     IAPQWNLTKNEILTGIRDELARRGTYQGFFVE"
     gene            complement(8885..10870)
                     /locus_tag="PLO58_07320"
     CDS             complement(8885..10870)
                     /locus_tag="PLO58_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQO74637.1"
                     /translation="MMAPTNTPLGSALFIVLLVVALSWFVWLMYRRIAVIAAGQPQNR
                     FDHIGRRIGAFFTQGIAQTKIRDRRFWSAGYMHAFIFWGFLVVSINTIQMIGQGFNPN
                     FVLPGFHTGQTLGQIYLWLKDVFEVLVLLSVIYALIQRLIIRPKRLTLSASANLILIM
                     IAILMVTDLLMTGLTVTFEADADRTVVGVAFARIFRNTSPEFTVKFFHLNWWLHLITL
                     LSFFPFLPLSKHFHIITSLPAVFFKRLDSGYVRFLDVENGERFGASRIEHLGWKDLLD
                     VFTCTECGRCQTVCPAFESGKPLSPKEFNIALRHHLIKYTTDIIKDKRQKSQSPRAEK
                     LVGDTIGEETLWACTTCRACEEVCPLSIEFIDRIVEMRRHLVLEESRFPEAAELAFRN
                     LESYGNPWGASDREKWTEGLAIPRAEEKADFEYLFWVGCAGAFDEHAQKTSRAMIKLL
                     NAARVNYAILGNAETCTGDAARRLGNEYLFQTLANQNIETFHNLGVQKIITQCAHCYN
                     TLKNEYPQLGGKFEVISHIEMIANLLAAGRLKINQPLKGKLTYHDACYLGRHNGIYDA
                     PRQIIAALQADFQEATRHGSNALCCGAGGGRMWLEEAPAQRVNTLRAKELTTLNPDII
                     ATACPFCTTMLYDGLKEQNSSVKCLDLAVLVASQLAE"
BASE COUNT         3067 a         2169 c         2056 g         3539 t
ORIGIN      
        1 agtaattcgt tacgattttt accggctaat caatttcaag aattcagcac gcgcttgcgg
       61 ttcatcatgc agttcaccga gcataacgct ggtagtgata ttggaatttt gtttttcgac
      121 gccgcgcatt tgcatgcaaa gatggcgcgc ttcaatgatg acgcccacac cttttggttc
      181 gagcgcttca ttgagggcat cagcgatctg ctgagtcagg cgttcctgaa tctgtagccg
      241 ttgcgaaaaa gccgtaacga cgcgtggaat cttactaaag ccgatgattt ttccattcgg
      301 aatgtaagca acgtgggctt tgccgaaaaa tggtacaagg tgatgttcac aaaggctgaa
      361 gaaatctata tctttgacga tgatcatctg gttataatct tcgtaaacga gtgattcttt
      421 gagcatttca agcacgtcca ctttatagcc ttgcgaaacg tgttgccaga atttggcgac
      481 gcgcgcgggc gttttcagca gttccggccg gttggggttt tcaccaatat tgatgagtaa
      541 attgcggatg atttcttcag tttccatata acctcagttt aatgtttatt cagattttaa
      601 ttgttgtaat ttttgctcta attcaaatga tttattttga cttagaattt caccattgat
      661 ccaattcacg cgtactccat ccattgttgg ccgactcatt catatagaag gagcgcgcct
      721 ggggattctc aattcttagt aacaagcgat aatgagtcca ggttaattct gggcggatcg
      781 aattacccaa tcttataaca cttttgctat ctccattagc aagactatct gataattctc
      841 gacgcactgc gtcgagaatt ggaaatagga gataaaattt gcgcatatac cacaagttac
      901 ttggatcaaa tccccgaccg tatgcgaccg ttagttttct ggaaaggtcg gcaattagtt
      961 gcttcccaaa ttccgccctt cttttacctt tttgttcctc atcaacgata atccgtccga
     1021 tattccaata gacttcaacc atgataaaat tgaccgatcg gtaagctctg gaacgggcat
     1081 cctctaaaat acttcggatg ttattgaaaa tagaatcgga gacttgagac aaattattca
     1141 tttagtaatt cttgctgagg ggcaaatatc attcaagaca catttgtcac attgtgggcg
     1201 tcgggcgatg caaacctgcc gaccgtgttc gattagcagg tgcccgagct tggtccattg
     1261 ttcttgggga aattggcgca tcaagtcctg ttcgattttt tccgggttgc tgtttttcgt
     1321 tagtccgagc agattcgcca gacgcttgac gtgggtgtca acaacgatag ccggtactcc
     1381 gaaagcatcg ccgagcacca cattagccgt tttgcgtccg acgccttcga gttgcaggag
     1441 ttcctccatt gttccgggga cttcgccgtt gaatttctcg acgacagatt tcattgcgga
     1501 tttaatggct ttggctttgt tattgtaaaa gccggttgag cggatgtcgt ttttcagttc
     1561 ttcgaggtca gcatcggcaa aggcttgcgc cgtcggatat ttctggaaaa gagccggtgt
     1621 taccatattg acgcgcttat cggtgcattg cgccgataga atggtggcaa ttagtagctc
     1681 ccgcggattg gtatgattca aagcgcagcg agcgtccgga taggcggcgg aaaggcggga
     1741 gaaaatttct ggtgcgattt cagatttatt agtgataatt ttcctcttag atttagttac
     1801 attaatttta gatgctgaaa aatttcaaca attccttcgt atcatccgaa atagtcagta
     1861 gataaagctt catctgcaat aaactttaac tcttcttgag ttaattttct atctaattcc
     1921 tgttcggcaa cagtctgaac gtccgcaata ttgattgaat agattatttt atcacttcgc
     1981 attttgctca cctccttcta aaaatggtct gataatatcc aaacgatttg tattgatttt
     2041 accatcaggt gagaatgggc actgagccag tattctcttt aaagttttat cctttattga
     2101 aagatcattc aagtggggaa aattttgaat aatttgtaat tcagtaaaca aaaaccaatc
     2161 gtgcaaaatt gctttacttt tggtagcagt ttgtgaaata ggctggttta ttatatcaaa
     2221 tttatccatg aaatcaggtt ttgcaattga attgattatt gagagaatca gttccattgt
     2281 tcctgtttct ccagaaagaa aattccataa ttcatcagca agtaatatct cctgtggatg
     2341 gaaatacttt ctaaaatcta tagaatatcc gaaatatctt tctttatttg attttgttgg
     2401 ttgtttactt gtcggatcaa atggaaatcc taagtaataa gacacttttt tgcctaaata
     2461 tttatttcta agaccagttt ttgcctgtaa tattttttct ttttctactt taaaaacacc
     2521 actgttcggt tttaccgttt ttaactctat agcaattatt tcattatcag tctcatagaa
     2581 aacatcggcc gtaaaatttg gtatgtggcg atctaatatt tcaccttgat atttaagaat
     2641 attgtcttct gaagaaacat caggctgtct ttgaccgttt ttaagttcag taataatatc
     2701 tgaaattact gtttcttgcc tactacttat aattaggtcc cggaattctc ttttttcacc
     2761 attagataaa atatgtgata ctcgttcaaa gaatgattga ccaagagttg tacttagccc
     2821 atgtaaccaa ctactaaggg ttatcaaaaa aggaacagat tttacctttc cctctaattt
     2881 aacggaaaaa gcatttaaga aagcttcatg aaaaggcgca tttctattgt ccatttcatt
     2941 ttctggaaaa gtatcaaagc gggatttgag ggtttttatt acctcaaaag caattttctc
     3001 aattttagca tttgtcattg aacgtccttc aaatgaaaga taatttctga ataggcacct
     3061 ttatcttttt ccgaacgatt taaaacaggc cttttataac agttgacgat tttcatcccg
     3121 gatttttcgg caatcatagg atataggtta tatttatcat tagccacaag aaaaatacta
     3181 taattgggtt ttaaatatct cttacaattt atcaatacat ctgtaatacc ctgaacgtac
     3241 gattctcgag cttctattcc tttcccttta aataaaggac caatttctaa ttcgtcattt
     3301 ctctcaaagc caaataaatc gtaggcgtag gcatgttgtt catgataatt gataagaccg
     3361 acgtatggtg gacttgaaaa aatgccgtct attttttttc tctgtactaa atccgacaat
     3421 tctttattct tgctttgtag tttttcaatt atatctatgt ttcttgaatc gcccacaaga
     3481 cagtattgga aggtattggt tcttaatttg ttaaaagttg ccaatctcac taaagtatcc
     3541 ttgctatatc tttcccacca attaagtatg gtaaaaagtg gtttgcatat tttaccatgc
     3601 ttggcacaat aatagggtgt tgctaccggt tcaattaagg tcactaaatc agcgtgggtc
     3661 gtagcgcggc aggagcgaat tgttctactg aatataacac tgattatctt ttttatttct
     3721 gaatcctgga ttaatttaat ttgttcaaaa acaaaatcca tttcttctcg aatcggctga
     3781 aggtaccact tttcaatgaa attattttca gtatcctgtt ttaatttgac gccatatttt
     3841 tttactaaat cccagtagat tggtagaaat tcatccgatt tagtttttcc gtaattatcc
     3901 tcattgattt ctttacgtct gaccttttgt ttatattcag gtgaagggaa atatctatta
     3961 ttaaattcat tgagtttttc agtcagttca acatcaaact gcagaatatt tctcttcgag
     4021 ttaaaaaccc ggagtgatcg tgttattttc gtaatttctg cctgtagaga gaacaaatca
     4081 tactttttaa cttttacatt actgatcaag gcattaaaag cagaaacatc aactcctata
     4141 gcgtgcattc ctaattcatt ggcttgaact aaagtggttc cacttccgca gaatggatca
     4201 aggattatgt cgcctttgtt aaaaaaaact tctgtcttaa actcgtcaat atgatcatca
     4261 agaaaatatt ctactagttg gggaataaat ttccctttgt atgggtgaag ccgatgaacg
     4321 tgtttggtgg tatcggcctc ttttaagaaa tcaaaactca aatgccaatt taaatcctct
     4381 cctaactgat ttttccaatt aatttcacgc ttaccccaga acgagtcgta atagtttttc
     4441 agctcggaaa tgtcaacgta aatcgagcca ttattactgg ttttatttat ttttccatag
     4501 ttaattaaat aggtgatgtt agattcggtg acttttttgt ttaagtaatc acttgcccaa
     4561 cggctggcat cttttagcga aagaatatta ttttggtttt taaataatgt ttccatttca
     4621 cccatttcaa ttcacctttt ccacccttgt aatttgcctt tttcggaggg ggaacaaggg
     4681 cttttcagat ctaaaactgt cattttccga tgttttgtga ctgacatact ttacataaat
     4741 catgtttata aacttcttct tctgattcag tgttttcgaa aagagataga tcactcttaa
     4801 ttacggtttc aggaataaaa atattcgggt ggagctttat tttggaatat tctatttcct
     4861 taatgttatc gggaattaca aaatattttg ttaactctac ccaatcttta tctctaattg
     4921 tgattggctc tcctaggaaa aaacttaacc aaattccatt gattttagaa cataattctt
     4981 cctgtacaag ttttttttga gtaatggtta tatagttctc atctatatca aagccaatat
     5041 actttcgacc aagtcgttta gccgcaattg ctgtcgtccc tgtgcctaca aaaggatcga
     5101 gcacaatgtc accttcatcg gaggacatta atattattct ttctaataaa tgaaccggta
     5161 actgacaggg atgttcatct ctatatttat tatgttttag gcgatgaata tcggtccaca
     5221 catcagaaac taaaggacca aagggatgta aaccagcctt tttgccgcca taatctttta
     5281 agagataccc gcattttctg catcgtttat gggggtagcg aatttcgaaa aacttatttt
     5341 tctttaggtc ttttgcataa tataaaatac cataatgaga aggttgtaaa ctttgaccca
     5401 ttggggcagt tggtgcatcc caggcaatcc aatgtttgaa ataagcaatt tcatttaaaa
     5461 aacttgcata gtatgttaac cacttgggta tattgtgcac aaatatagaa cccgcaggtt
     5521 ttgtaattct aaccatttca aatatccacc gtttgcacca atccaaatat gcctgaacct
     5581 ctttattgtc ccgataccca ttatatttct tacctaaatt aaaaggcggg tctgcgaaag
     5641 ttacatcaac actgttatcg gggatgtttt tgaataattc aatacaatcg ccttgaatta
     5701 tctgattgta atatttctca aacatttttc atcacttcac ataataattt ctcaaatcta
     5761 ttaagttcct cttgatgaaa ggtaaataca tcttcataag cgccaaccat cctttttaaa
     5821 tccctttttc taacatacca accaataccg tcaacaaagc ctaaaaagcg agcatctggg
     5881 taatgttcct taattaaaga gtcaattgag atctctgttt tcgatttatc accttgacct
     5941 gaagaagttg tgactaaaaa agtactttct attataattt ttgggctttg tttatttgga
     6001 ataataaaat ccattgttct cttttgttcg ggtgcatatt caataagttc gctgagatca
     6061 cctttttcaa aaggaatttt acgtttcttt agaattcttt cgataagaat ttctgggtta
     6121 tttttccctt tagcagctct cgaacctttt tctttatatc gtgcaagagt atcaatgatt
     6181 gcattgatat caaaatttag cttttcgaga cttaattttt gaaactcaaa cggcggtaag
     6241 gttcttttga aaaaatctat tgacgaacct tcataaaaaa tattgaccag acattttcta
     6301 aaatattcat tttcccgcac caattgctca attttggagg tattccattc cgaaatttca
     6361 ttatctttgg tcgttctttt attattaatc caataattca taaaagtaaa atcattcagc
     6421 tctttatcgt ccacaattct gataatagtg atcaacctta ataaactttc atttgagaat
     6481 cctgtaatgg cgagtaatgc cttaaggccg agttcttttt tattccttaa aaaattttcg
     6541 aagacttctt ttttacaacc attattctgt acttcatttc ttagatatat tatagattcc
     6601 ttgacagaaa aaatatgact ttcataggct gattcgaatt cttcatcaaa ataataatat
     6661 gtattattct caattactgt tctatacttc tcgtcgattg ttttcatatt tagttcctat
     6721 ctggattttt ctggaagaaa agtaaatact cgtctttcat ggtatttctc attcctttaa
     6781 ttggctttag gatgctttta actaacttta atccaatctc ttcacaattt tggattatag
     6841 ttttttctaa tcttatcccg ccggtttgat ttgtattcga accaattatc ataacgaaat
     6901 atttgtcatt tttcagcacc ctatatattt ctgaacagac ttttttcata tcacgaaaat
     6961 aattatttaa tctttcgtct ttatttttgc caattaaccc aatcatctta cctttaattt
     7021 tctcgatatt atacccaaga tattctaatt gggcttcatc attcttggca taatcaatcg
     7081 cgaaagaata agggggagat gtaattacgc catctattga gttatccacg atttttaaat
     7141 ctaaagcatc cgaatccgat aatacattca gtttacccag ttgtactttt ttgaaatagg
     7201 gattattaat aaaatttcgt acggtatttt catatctagt taaaactttt tcgaacaatt
     7261 gcctgtgatc cgatttaatg acacgttttg aataccccat tgaatccaag taagccaata
     7321 aagctaaatt atatatggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     7441 gatagggaaa tagtccatgg gtagcataac cagtagagaa agtaccgttt tcaaaataat
     7501 ttttggtgac attggatcga ttttcgatta gcccctcaca ggtttggtta tacattaaat
     7561 aatgcgttgt gaatttgcca tcaattgatt gaatataggc cgaacgttca ataatttggt
     7621 cttttggaag gcttttgtat tcgagaaaag ccaactcgag ttcaaataat tctgtaacag
     7681 atggtaagag agttacgttt ttatcaaata gagtttctat acaggctggt aattcataga
     7741 aggaataatt tcccaaaact ttcataacct ttcattaaca attgataaag ccatttcacc
     7801 cacttttaat tcaccttttt cacccttgta atttgcctta tttgtgagca gtaatcaagc
     7861 gctttttatg attggtattt gcgatcattt ttcaacgagt tctattcgac aaaaaatcct
     7921 tgatacgtgc cgcggcgcgc cagttcatcc cggatcccag ttaatatctc gtttttggtg
     7981 agattccact gcggggcaat cagcgtgccg ggtttggcat cgccgcacaa acgctggatg
     8041 acgatttcgg gatgtaagcg ttccaggaaa tcagcgacaa gcgttatcca ttctttttcg
     8101 gtaaaaagtg agaagggatt ttcggcgtag aatttagcca gaggcgtgtt tttgacaacg
     8161 tgcaggtggt gaatcttgag cgcatcaata ccaagatcgt tcaagacgcc gggagtagct
     8221 aaaatctcag cgcgcgtttc agtcggaaat ccgagaatta ggtgtccagc aacgtgcaga
     8281 ccgtgggctt tagccgcctg aatcgcccag cgagtttgag caaaatcgtg tccgcgctgc
     8341 gcccaatcca gcgttttatc atagacggat tcaataccga actcaatgga aatgtaagtt
     8401 tcgtgattca ggtgttcgag tagttgtaat atttcctccg aaacgcagtc cgggcgcgta
     8461 ccgatgcaga ttcctacaat ttcagaaaaa gctaaaacct cgtggtaaag tctttccagc
     8521 tttgcgaccg gcgcataagt attggtaaac ggttgaaaat aagccagaaa tttatcggcc
     8581 tggtgaaaac ggcgagccga cgctatgcct ttttggattt gatcgctaag cgacggcaat
     8641 tcttcagcac gctttggggc gaagctttcg ttattgcaat acagacagcc ggaagtgcct
     8701 ttagtgccgt cacgggttgg gcacgtaaaa ccagcatcga gcgtaatccg ataaacccgc
     8761 gtctgaaagc gctcgcgtag gtagcgacca taacgataat agcgttcatc cagcagaaag
     8821 ggaatcactt tttaattaaa atttcagatt taagaatctt taatttcaaa tcgaaattca
     8881 ccggttattc agctaattga ctggcgacca gcaccgccag atcgaggcat tttacgctcg
     8941 aattttgttc ctttaatccg tcataaagca tagtcgtgca gaaaggacag gcggtggcaa
     9001 taatatccgg attcaatgtg gtgagttcct ttgcccgcag ggtattgacg cgctgagctg
     9061 gggcttcttc cagccacatc cgtccaccgc cggcgccgca acatagagca ttactaccat
     9121 ggcgagtcgc ttcttgaaaa tcagcttgta atgccgcgat tatctgacgc ggcgcatcat
     9181 aaattccatt gtgccgtccg agataacagg cgtcgtgata ggtcaatttg ccttttaacg
     9241 gctgattgat tttcaaacgt cccgccgcta acagattggc aatcatttcg atatgactga
     9301 taacctcgaa tttgccacct agctggggat attcattttt cagagtattg tagcaatggg
     9361 cgcattgggt gataattttt tggacgccga gattgtggaa agtctcgatg ttttgattgg
     9421 ctaaagtctg gaaaaggtat tcgttaccaa gtcggcgagc ggcgtcaccg gtacaagttt
     9481 cggcattgcc cagaatggca taattgaccc gagcggcgtt cagcaatttt atcatcgcgc
     9541 gggaagtttt ttgggcgtgt tcatcgaaag ctccggcgca tccaacccaa aagagatatt
     9601 caaagtcggc tttttcctct gcccgcggaa ttgccagacc ttctgtccac ttttcgcgat
     9661 cagaggcgcc ccagggatta ccgtagcttt ccaaattacg gaatgccaat tctgccgctt
     9721 ccgggaaacg gctttcttcc agtaccaggt gacggcgcat ttcgacaatc cgatcaatga
     9781 actcgatgct cagtggacag acttcttcac aagcccggca ggtggtacaa gcccaaagtg
     9841 tctcttcgcc gatggtgtcg cccaccagtt tttccgcgcg tggcgattga gatttttgtc
     9901 ttttatcttt aataatatca gtcgtgtact tgataagatg atgccgcaaa gcaatgttga
     9961 attccttcgg actcagtggc ttgccggatt cgaacgccgg gcaaaccgtc tgacagcgac
    10021 cgcattccgt acaggtaaaa acatccagca aatctttcca gccgagatgt tcaatgcggg
    10081 acgccccgaa gcgctcgccg ttttctacat ccaaaaagcg cacatatcca ctgtcaagcc
    10141 gtttgaaaaa aaccgctggc aaggacgtta taatatgaaa gtgcttggaa agcggcagaa
    10201 atgggaaaaa ggacaacaga gttataagat gtaaccacca attaagatga aaaaatttta
    10261 cggtgaactc cggactggta ttgcggaaaa tacgggcaaa ggctactccg acgaccgtcc
    10321 gatcggcatc cgcttcaaac gtgacggtca acccggtcat taaaaggtct gtaaccatca
    10381 aaatcgcaat cattatcaaa atcagattcg ctgaagcgga taatgtcagg cgttttgggc
    10441 gaatgatcag gcgctgaata agggcataaa tcacgctgag tagaactaaa acttcgaaaa
    10501 catctttgag ccagagataa atctgtccga gagtctgtcc agtatgaaaa cccggcagga
    10561 cgaaattggg attgaatccc tgcccaatca tttgaatcgt attgatcgaa actaccagaa
    10621 agccccaaaa aataaatgca tgcatatagc cagccgacca gaaacgccga tcacggattt
    10681 tcgtttgagc aattccttgt gtgaaaaaag cgccgattcg tcgtccgata tggtcaaagc
    10741 gattttgggg ctgaccagcc gcaattacgg cgattctccg atacatcagc caaacgaacc
    10801 agctgagtgc gactaccagc agaactataa aaagtgccga tccgagtggg gtatttgttg
    10861 gtgccatcat ttaccagttt ctcctttttt aaggtgaaat tttaaacatt tccagcttga
    10921 ttccaaaatc a
//
LOCUS       DAOMJU010000072        10790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6246_length_10790_cov_15.284304, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000072 DAOMJU010000000
VERSION     DAOMJU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6246_length_10790_cov_15.284304"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLO58_07325"
     CDS             complement(<1..494)
                     /locus_tag="PLO58_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3308 domain-containing protein"
                     /protein_id="HQO74638.1"
                     /translation="MKTIFKSIVIGIVVGCLTIPVELSAKNEKLAQAGFEFLSVVSDA
                     RAAAMADAMTSLQIGSSALFFNPAGMANIEQLIDLTASNNQWITDIQHNTFSLAFSPT
                     HGRYGVIGLSYQNVNYGDFYGTRVNMRVDAGYDDTGVFQLSAHALGLGYAKRLTEWFS
                     VGGQ"
     gene            complement(508..2769)
                     /locus_tag="PLO58_07330"
     CDS             complement(508..2769)
                     /locus_tag="PLO58_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74639.1"
                     /translation="MFKTKIKNHLLFLISAVTVVLMFSNTTFAQDQRWIIVGETQCFF
                     MDYGAETERELNFLTWPTQYGDNQHTLRMNGLWLGARNFFDPVENKEKSVKVIGSGPR
                     LPANHYTMIFPQSIKLIGRHSHPVVAVDNQTGTNNLLYDQVDDYDSNLPCDRMVVAEF
                     NTSMGISVTRKVYGFTQPDHDNYFIQEYVFKNTGIYNENGDVHHQDLQDFWVYLCWRP
                     AFAGVTSSGWTDQPAWGAFASEWGSSTLIHTFNVNSPEGMRGFYSYYGPTNDSNHPLT
                     PAEDWGCPDHLETGVLGSAKYLGGVVLFASNPNNHTVDDPSQPATDAYIGSDGVPMET
                     NVSQYDVSFMQQRYNIMTEGHLPASQDEDFFNSVGHNGYVEEWTTGNTYRNTYTQGSS
                     SQGWGFGPYNLADGDSIRIVFAIGVTGLSWEKCREVGANWYAYYKSLTNKPDLYMPDG
                     SLASTTNSWGYAAHNAYKRAWCETGRDSILQVLSNAIDNYNKGYNVPQPPPPPESFTV
                     TSGGDRIRLTWSDNAVNSPNFNGYVIYRSEGNVKHYKTVYTKIFECDKASCVHEFDDV
                     TAARGNNYYYYIQSKSDGQENNGKPLYSSLFYTLTSVAAYLRRPAGNFLEHVRVVPNP
                     YDIRARMWQFGEDFQYDRIAFYELPPICKVKVFTERGDLIWEKYHDNGSGDELWDSMT
                     SSRQIIVSGIYILYVEVPQDIYAEQDIVAARDYYNPKTGEKEISKGDVIFQQGDKMYN
                     KGDSVFRKFVVIR"
     gene            complement(2794..6027)
                     /locus_tag="PLO58_07335"
     CDS             complement(2794..6027)
                     /locus_tag="PLO58_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO74640.1"
                     /translation="MKKIIRIIIPAFLLATCLVSLLWSGTTGKITGIITDKKTGEPII
                     GANVIVMGTSLGAATDQNGQYTILYVPPGVYNVNISVIGYAKTTLSDVEVHIDQTTRL
                     DVNLEEEAVVGETVVVVAERKIIKRDVATSSVAVSANEIEILPASNVDDVISMQAGIR
                     GNMAIRGSEAEDMLFLVDGITVRDPRNNEPVTKIPLSAVKEISVERGGFNAEYGQVQA
                     GLVNVVTREGDKSNYSGNFQIKYSPPAPKYFRVDGIPDVHDPDSYWMRPYLDDAVCWT
                     GTTQGEPFTDLNGNGKWDTGEDFEDLNSDGSHTFWDQYMRDQYPSFVGWNEISRQLLT
                     DNDPTNDLTPLGAQRVFLYETRKKQVNDMPDYDIDAGFGGPVPLISEKLGNLRFYTSY
                     RRHRDVLLWAQATPDYVDYDWTMQLTSDISETMKLRISSLLGSISTIAENWNYAYYPH
                     WPNEIANGTGGLLMFNMFSDWAYSKTDIGHRSFAIKLTHTLSSKTFYEVSLENFQRDY
                     HTRPIGLRDTTKTTEIVPGYWVDEYPFGYWPYEVVGITPEIKDGIQASLARDYSNASA
                     TTFKADLSSQVNFHNLIKTGVEFVYNDLDLDYGFIQMQTKGQAYASRVQMQNFPIRAA
                     FYIQDKLETDGFTMNAGLRLDYSDSRVEWWDIFSDPFNPYFISSKYKEDLEFDMVKSK
                     PQWQLSPRLGISHPITENSKLFFNYGHFKQMPQYETLFRVQRSSTGSLRAIGDPNLTL
                     AQTISYELGYDHLLFNSVLVQLAAFYKDISDQQNETTYESINNDTYILITSNGYEDIR
                     GFELTLRKSQGRWFTGLINYTYQASSRGYFGRREIYQDPAHQQRYDEETSNLYQERPV
                     PTPYARANLSFYTPDDFGPVVLNHHILGGFMLNLLLTWEQGGWTTYNPKNASGVINNV
                     QYLDYYGGTLRAGKTVTSKRFRIQFLVDVNNLFNRMTLWDTGDQKYRESLHLPKSDAY
                     GNIPGDDKLGDYRKPGIEWQPMKYQEIIQGTIPPALNTNTIPIYYEGSTGTYWEIVDN
                     QWIEVSKSKIDQINADKAYIFNPGASTWWFLNPRTVVFGVRLSFDLD"
     gene            complement(6368..8182)
                     /locus_tag="PLO58_07340"
     CDS             complement(6368..8182)
                     /locus_tag="PLO58_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO74641.1"
                     /translation="MRMRTVVISVVLIMALSSIAMPQSLVRESFSYPATTLAGLGTAS
                     NGFSGSWVTDEAGGIEGLVAVSGTRFAYADLNYEIPHDTLHLQVVKSNAWADHNRYKR
                     PLDGAWPNEAGKKYWVSYLLDIKEPLPIENTYFMVKLYKGSGELLAIGKAGGGPTPPV
                     FTCGSGWVGEGDDVSATPITAGPVWIVTMVKMSGGTGSDRTYMWIDPDPSVEPDTNDA
                     IVKRNSSMPEGFDHIALEFGGDGADVRLVFDEITVASSFADLTAETPTGTVARESFSY
                     PATSVAGLGSAGSGFSGPWVTDEAGGIEGLVAVSGTRFAYADLNYEIPHDTLHLQVVK
                     SNAWADHNRYKRPLDGAWPNEAGKKYWVSYLLDIKEPLPIENTYFMVKLYKGSGELLA
                     IGKAGGGPTPPVFTCGSGWVGEGDDVSATPITAGPVWIVTMVKMSGGTGSDRTYMWID
                     PDPSVEPDTNDAIVKRNSSMPEGFDHIALEFGGDGADVRLVFDEITVASSFADLTKTS
                     GISRKETVSFEYSLSQNYPNPFNPTTNITYSLKQTGDVRLSIFDILGREVAVLVDGVQ
                     NAGQHTITFTGTDLSTGIYLYRLQVGKEVITKKMVLVK"
     gene            complement(8549..>10790)
                     /locus_tag="PLO58_07345"
     CDS             complement(8549..>10790)
                     /locus_tag="PLO58_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQO74642.1"
                     /translation="YRESVLRASNGMLYFGGINGFNVFQPGSISYNPFIPPVVITQFQ
                     IFNETISPNTQGKIKLDSSIFITENIILSYRENVFSFEFAALDYSCPQKNQYAYIMEG
                     FDQDWRRTDANRRFATYTNLPPGNYTFRVKASNNYGVWNESGTAIHLKITPPFWQTNW
                     MLIIYIGLILGAILLIRMAVQVRERNRAQAEMRRLEAEKTHELDQLKLRFFTHISHEF
                     RTPLTLIIGPVERMLHLGNKLSQEKRDVYNRLVLQNARRLLRLVNQLMDARKLDTGSM
                     KLELQQKEFISFVRAIFSVFHLQAEQRNIRYIFKTELEEQYFYFDPDKIEKALYNVIA
                     NALKFTNPGGMVEVSLFLAPPNMESYSKREEAERSICVQVRDTGIGIPPDQIEHIFDP
                     FYQVRQPETRKMHGTGIGLSISRDFIEMHGGRIIVESTPGKGSTFSLIIPMRTMAEAL
                     KTGEQEVGEQDYIGEMAELIPDENLKISNELTESKLSMKNSDKAKPLILIVEDDNDLR
                     HYLAMELESYYSVIQAADGLTGYELAVDRIPDLIICDVLMQVMDGNEFCHRCKNDQRT
                     SHIPIILLTAQTSEEKRKEGLLAGADDYITKPFNPDLLRIRIENLLNTRRKIRERFGH
                     MVYMNPKEINVSSPDEQFLQRTVEVVEQYLSDSGFSVDDLSYHVGLSRAQLYRKMQGL
                     INQTPSEFIRNYRLQRAMQLLEKGHSPSQVCYQIGFKDPSYFTKCFRKQFGRTPQQIR
                     PESELS"
BASE COUNT         3081 a         2527 c         2139 g         3043 t
ORIGIN      
        1 atttgaccgc ccacactgaa ccactccgtc aagcgcttcg cataacccag tcctagggcg
       61 tgggcactca gctgaaagac gccggtgtca tcatagccag cgtcaactct catatttacc
      121 cgcgtgccat aaaaatcccc ataattcaca ttctggtaac ttaagcctat cactccatat
      181 cggccatgag tcggactaaa cgccagacta aaagtattat gttgaatatc cgttatccac
      241 tgattattac tggcagttag gtcaattaat tgctcgatat tagccatacc tgccggatta
      301 aagaatagag cgcttgaacc gatctgaaga cttgtcattg catccgccat cgccgccgct
      361 cgagcatcgg ataccacact gagaaattca aaaccagctt gagccaattt ttcgtttttg
      421 gcggataatt cgacaggtat tgtcaaacat ccaactacga ttccaataac tatagatttg
      481 aatattgttt tcattttccg cccctcctta cctaatcacc acgaatttgc gaaacaccga
      541 atcgccctta ttgtacatct tatcaccttg ctggaaaatc acatcgccct tgctaatttc
      601 tttttccccg gttttcggat tgtagtaatc acgggcggct acgatatcct gttcggcata
      661 gatgtcctgc ggtacttcca cgtaaaggat atatatacca ctgacaataa tctgacggga
      721 agaagtcatc gaatcccaca attcgtcgcc ggaaccgtta tcgtgatatt tttcccagat
      781 taggtcgccc ctttcagtaa aaacctttac cttgcagata ggcggtaatt catagaaagc
      841 aattcgatca tactggaaat cctcgccgaa ttgccacatt cgagcgcgaa tgtcgtaggg
      901 attcgggaca acacgtacgt gttccaaaaa gtttccagct gggcgacgca gataagccgc
      961 tacgctggtc agtgtataaa agagactgct gtatagcggt ttgccattgt tctcttgacc
     1021 gtcactctta gattggatgt agtaatagta attattaccg cgagcggcgg tgacatcgtc
     1081 gaactcatgt acgcaacttg ctttatcgca ttcaaatatt tttgtataga cggttttata
     1141 atgtttgaca ttgccttctg agcgatagat gacatatccg ttaaaattag gcgaatttac
     1201 ggcattgtca ctccaggtca agcgaatccg atctccacca gaagtaaccg taaatgattc
     1261 gggtggggga ggcggttgcg gaacattgta tcccttattg taattatcta tagcattact
     1321 taggacttgc agaatcgaat cgcggccggt ctcgcaccag gctcgtttat aggcattatg
     1381 ggccgcgtag ccccaggaat tggtagttga tgctagcgaa ccgtcgggca tatacaaatc
     1441 cggtttgtta gttagacttt tataataggc ataccagtta gccccgactt ctcgacattt
     1501 ttcccagctt aatccggtca ctccgatggc aaatacgatt cggatgctat cgccatcagc
     1561 cagattataa gggccaaatc cccatccttg actgctactg ccttgggtat aggtgttccg
     1621 gtaagtattc ccagtagtcc attcctccac ataaccattg tggccaacag aattgaaaaa
     1681 gtcttcatcc tggctagcag gtaaatgacc ttcggtcata atgttatacc gttgttgcat
     1741 aaaactgaca tcgtactgac tcacattagt ctccattgga acgccatcgc tgccaatgta
     1801 ggcatccgta gctggctggc tggggtcatc aaccgtatga ttattcggat tcgatgcaaa
     1861 tagaacgaca cctcctaaat acttagccga accaagcaca ccggtctcta aatgatcagg
     1921 acaaccccaa tcctcagccg gagttagtgg atgattagaa tcattagtgg gaccataata
     1981 actataaaat cctcgcattc cttcaggaga attaacgttg aatgtatgaa taagagtact
     2041 gctaccccat tccgaggcaa aagcgcccca agccggttga tcagtccagc cagaggaagt
     2101 cactccggca aatgccggcc tccaacacaa ataaacccag aaatcttgca gatcctgatg
     2161 atgtacgtcg ccattttcgt tatatatgcc cgtattttta aatacatact cctgaataaa
     2221 ataattatcg tgatccggct gagtgaaacc ataaactttt ctggttactg atatacccat
     2281 cgaagtattg aactcggcaa ctaccatacg gtcacatggc aggttactat catagtcatc
     2341 aacctgatca tagagaagat tattagtgcc tgtttgatta tccacagcaa ccaccgggtg
     2401 actatgtcgc ccaataagtt tgatactctg cggaaaaatc atagtgtaat gattcgccgg
     2461 gagtcttgga ccagatccga ttactttcac tgatttttcc ttattttcga ctggatcgaa
     2521 gaaattgcga gctcccagcc acaatccatt catccggagc gtgtgctggt tgtcaccata
     2581 ctgagtaggc caggttagaa aatttaattc gcgttcggtt tctgcgccat agtccataaa
     2641 gaagcactgc gtttcaccga cgataatcca gcgctggtct tgagcaaaag tcgtgttaga
     2701 aaacattaaa acgactgtca ccgcactaat tagaaataag aggtgatttt ttattttcgt
     2761 tttaaacatt ggtattcctc atttctttta taattaatcc aaatcaaaag aaagccggac
     2821 gccaaaaacc actgtccgcg gattcagaaa ccaccaggtt gatgccccgg ggttaaaaat
     2881 ataggcttta tcggcattga tctggtcaat tttggatttg gaaacctcta tccattgatt
     2941 atcaactatt tcccaatatg ttccagtgct accctcataa taaattggaa tggtattcgt
     3001 attaagagca ggtggaattg ttccttgaat gatttcctgg tatttcatcg gttgccactc
     3061 aattcccggt ttacggtaat caccgagttt atcatctcca ggaatattac cataggcatc
     3121 actcttgggc agatgcaatg attcacgata cttctggtcg ccggtatccc ataatgtcat
     3181 acgattaaat agattgttaa catcaaccaa aaattggatc ctaaatcgtt tactggtaac
     3241 ggtcttgcca gcccgtagag ttccaccata ataatctaaa tactggacgt tattgataac
     3301 acccgatgca tttttgggat tataggtagt ccagccgcct tgctcccaag tcaataaaag
     3361 gttaagcata aaaccaccca ggatatggtg gttgagaaca accggcccga aatcatccgg
     3421 agtatagaag ctcaggttgg cacgcgcata gggagtgggc accggtcgtt cttgatataa
     3481 attactggtt tcttcgtcat aacgttgttg atgagccgga tcctgataaa tttcgcgccg
     3541 tccaaaatag ccgcgagacg atgcctgata ggtgtaattt atcagaccag tgaaccagcg
     3601 cccctgactt ttacggagtg tcagttcaaa accacgaata tcctcgtaac cgttactggt
     3661 gattaaaata taggtatcgt tattaataga ttcgtaagtt gtttcatttt gctgatcgct
     3721 aatatcttta taaaacgcag ccagttgaac aagaaccgaa ttgaaaagca ggtggtcgta
     3781 accaagttca taggagatag tctgggctaa agttagatta ggatcgccga ttgcccggag
     3841 actgccagtt gaactgcgtt gtacccgaaa cagggtttcg tattggggca tttgcttaaa
     3901 atgcccgtag ttgaaaaaca atttggaatt ttcggttatg ggatgtgaaa tacccagccg
     3961 gggacttaat tgccactgcg gtttggattt taccatatca aattcgaggt cttctttata
     4021 tttggatgaa atgaaatagg gattaaatgg gtcagaaaaa atatcccacc actcgactcg
     4081 cgagtcgcta tagtccaacc ttaatccggc attcattgta aaaccatcag tttccagttt
     4141 atcctggata taaaaggcgg ctcggattgg gaaattttgc atttgcaccc ggctggcgta
     4201 ggcttgacct ttagtttgca tctgaataaa accataatcg agatccagat cgttatagac
     4261 aaattccaca cccgttttaa tcaaattatg gaaattaacc tggctggaaa gatcggcttt
     4321 gaaagttgta gcactggcat tcgaataatc acgagccaat gaggcctgaa tgccgtcttt
     4381 aatctcggga gtaattccga ctacttcata aggccagtaa ccgaaagggt actcatcgac
     4441 ccagtatcca ggcactattt cggtggtttt agtcgtatcc cgtaatccaa ttgggcgggt
     4501 atgatagtca cgctgaaaat tttccagtga aacttcatag aaggttttag aacttaaggt
     4561 gtgagttaat tttattgcga aggaacgatg accgatatca gttttagaat aagcccagtc
     4621 actgaacata ttaaacatta gtaagccgcc ggtgccgttg gcaatttcat tcggccagtg
     4681 agggtagtag gcataattcc agttttcagc aatagtagag atgctcccca gcaggcttga
     4741 aatgcgtaat ttcatcgttt ccgaaatatc cgaagttagt tgcatagtcc agtcataatc
     4801 aacataatcg ggagtagcct gtgcccatag taaaacatca cggtgacgac gatacgaagt
     4861 ataaaaccgg agattgccga gcttctcgga aatcagtggc accgggccac cgaatccggc
     4921 atcaatatcg taatcaggca tatcatttac ttgcttttta cgggtttcgt acaaaaacac
     4981 ccgttgagct cccagaggtg tcagatcatt tgtggggtca ttatcagtca atagctggcg
     5041 agaaatttcg ttccagccga caaacgacgg atactgatca cgcatatatt gatcccagaa
     5101 ggtatgactt ccatcactgt ttaaatcttc aaaatcttca cctgtatccc atttcccatt
     5161 gccgtttaaa tcggtaaaag gctcaccctg agtagttccc gtccagcaga cagcatcatc
     5221 gagataagga cgcatccaat aggaatcggg atcgtggacg tccggtatgc cgtcaacccg
     5281 aaaatattta ggcgccggcg gagaatactt tatctgaaaa ttaccactat aattgctttt
     5341 gtccccttct ctagtaacaa cattgaccaa tccggcttga acctgcccat actcggcatt
     5401 aaaacctccc cgctcgaccg aaatctcctt caccgcactc aatggaattt tggttactgg
     5461 ttcattatta cgcggatcgc ggaccgtaat tccgtctacc agaaatagca tatcctcagc
     5521 ttcactaccc ctaattgcca tattgcctct gatacctgcc tgcatactaa tgacatcgtc
     5581 aacattcgat gccggtaaaa tttcaatttc attggctgaa acggctacac tactagtagc
     5641 cacatctctt ttgattattt tacgttcagc cacgactact accgtctcac cgacaaccgc
     5701 ttcttcctcc aagttcacat ccagcctagt agtctggtca atatgtacct cgacgtcact
     5761 aagcgtcgtt ttcgcatatc cgattacgga aatattgacg ttataaacac ccggtggcac
     5821 atacaaaatc gtgtattgcc cattttggtc agtggcggct ccgagagaag ttcccatcac
     5881 gatgacattg gctccaatga tgggttctcc ggtcttttta tcggtaataa taccggtaat
     5941 tttcccggtc gttcctgacc aaagcaagct caccaaacaa gtggctaaca aaaaggcagg
     6001 aattattatc ctaataattt ttttcattct caattcctcc tttcagagag aaaacggaaa
     6061 aatcctgaga tggatttagc aaaatttgaa cataaatcac acattttatc tttttaaaaa
     6121 acctcataaa ttcttttaac cttgcgatat tttcggtcgc ttcagattaa ttatttctta
     6181 tgctcgatta atattcgtct cgttaattga atcgagtaaa tagccgtatt gtcaaagaac
     6241 aactttattg catttaattg aacaggatag gggaaggcgc ttttcaacgc cttcccctgt
     6301 tgtcacccct cattgatcaa aatgtgaaaa aaccaaacga atttcacatt cgccgaattg
     6361 aaaaccgcta ttttaccaaa accattttct tggtgattac ttctttgccg acctgtagtc
     6421 tatacagata aatgcccgtg gataagtcag taccagtaaa agtaatcgta tgttgtccgg
     6481 cattttggac accatctact aacaccgcaa cttctctacc caaaatatca aatattgaca
     6541 ggcggacatc gccagtctgt ttgagagaat aagtaatatt ggtcgtcgga ttgaacggat
     6601 tgggatagtt ctgagacaaa gaatattcaa atgatactgt ctctttacga cttattccac
     6661 ttgtcttagt taggtcggca aaagaggaag cgaccgtgat ttcgtcgaag actaacctga
     6721 catcagctcc atcgccacca aattccaatg caatatgatc aaatccttcc ggcatcgaag
     6781 aattacgctt tacaatagca tcattggtat ccggttctac gctcggatcc gggtctatcc
     6841 acatataagt ccggtcagaa ccagtgccac ctgacatctt caccatcgtt actatccata
     6901 ccggtccagc tgtaattgga gtcgcagata catcgtcacc ttctccaacc caaccacttc
     6961 cacaggtaaa taccggcgga gtaggaccac cacctgcttt acctatcgct aacaattcac
     7021 cactaccttt atacaacttg accataaaat aggtgttctc aatcggtaga ggctccttaa
     7081 tgtccaacag ataactgacc cagtattttt tgccagcctc attcggccaa gctccatcca
     7141 agggcctctt gtaacgatta tgatcagccc aggcattact cttaactacc tgcagatgaa
     7201 gcgtatcatg tggaatctca tagttaagat cagcataagc aaatctcgtc ccactaaccg
     7261 caactaaacc ttcaatgcca ccagcctcgt cagtcaccca tggtccacta aatccactac
     7321 cggctgaacc taaccccgct acggaagttg ctggataact aaaagactca cgagcaaccg
     7381 ttccggttgg tgtttcagcg gttaaatcag caaaagagga agcgaccgtg atttcgtcga
     7441 agactaacct gacatcagct ccatcgccac caaattccaa tgcaatatga tcaaatcctt
     7501 ccggcatcga agaattacgc tttacaatag catcattggt atccggttct acgctcggat
     7561 ccgggtctat ccacatataa gtccggtcag aaccagtgcc acctgacatc ttcaccatcg
     7621 ttactatcca taccggtcca gctgtaattg gagtcgcaga tacatcgtca ccttctccaa
     7681 cccaaccact tccacaggta aataccggcg gagtaggacc accacctgct ttacctatcg
     7741 ctaacaattc accactacct ttatacaact tgaccataaa ataggtgttc tcaatcggta
     7801 gaggctcctt aatgtccaac agataactga cccagtattt tttgccagcc tcattcggcc
     7861 aagctccatc caagggcctc ttgtaacgat tatgatcagc ccaggcatta ctcttaacta
     7921 cctgcagatg aagcgtatca tgtggaatct catagttaag atcagcataa gcaaatctcg
     7981 tcccactaac cgcaactaaa ccttcaatgc caccagcctc gtcagtcacc catgatccac
     8041 taaatccatt actcgcagtt cccagccccg ccagagtagt cgctggataa ctaaaggatt
     8101 cacgtactag tgactgaggc attgcaattg agcttagcgc cataatcaaa acgacactaa
     8161 taacaacagt tctcattcgc ataatttctc tcctttgttt gtttttttaa tgcaattaaa
     8221 cgttagaaac aacattctta gtgatttgta gaacccacac ctcctttcgt cttttttaat
     8281 tatcatgaaa aaacctcact cttagattga gtgtttatac aatatcacaa aaaaaacgat
     8341 gatgaatatg atacaaatga cctcaatcag aatccatttc caacgattac gattttcggc
     8401 gctttcaaca gggtctggtt tgtttaattt ctgtttcatt tgaaactaaa gtaaagtgtt
     8461 taaaaagaaa aatcatctca aaatctggta attaggttgc ataccgattt taaaggtgaa
     8521 atttgatttt aaataaagaa tggaagggct aagaaagctc ggattcggga cggatttgct
     8581 ggggtgttcg tccaaattgc ttgcgaaagc atttagtgaa ataggaaggg tccttgaaac
     8641 caatttggta acaaacttga gatggcgaat gccctttttc cagtaattgc atagctctct
     8701 gcaagcggta attacggata aattcactgg gagtctggtt aatcaaacct tgcattttac
     8761 gataaagctg ggcgcgactc aagccgacgt gatagctaag atcatcaacg gaaaaaccgc
     8821 tatccgataa atattgttcg acaacctcaa ccgttctctg cagaaattgt tcatcaggtg
     8881 aactgacgtt aatttctttc gggttcatat aaaccatatg gccaaaacgt tctcggattt
     8941 ttcggcgagt gttcagtaaa ttttcgattc gaatccgcag aagatctggg ttgaaaggtt
     9001 tggtgatata gtcatccgca cctgctaata aaccttcttt gcgcttttct tccgaagttt
     9061 gagccgttag caggattatg ggaatatggc tggtacgctg gtcattttta catctatggc
     9121 aaaattcatt tccatccatc acctgcatta aaacatcgca aataatcaaa tccggaattc
     9181 gatctaccgc taattcatag ccagttaaac cgtcggcggc ttgaattacc gaataatagc
     9241 tctcgagttc catcgccagg taatgtcgca ggtcattatc gtcttcaact attaagataa
     9301 ggggcttagc tttatcacta tttttcatag acaatttgga ctcggtaagt tcattcgaaa
     9361 ttttcaggtt ttcatccgga attaactcag ccatctctcc aatatagtct tgttcaccaa
     9421 cctcttgctc gccagttttt aacgcttcag ccattgttct catcggtata atcaaagaga
     9481 aggtactacc tttgcccggc gtgctttcta cgatgattct gcccccgtgc atctcgataa
     9541 aatctctgct aattgaaagg ccaatacccg tgccgtgcat tttcctggtc tccggctggc
     9601 gcacctggta aaagggatca aaaatatgtt caatctgatc tggcggaatg ccaatgccgg
     9661 tatcacgtac ttgaacgcag atcgaacgtt ccgcttcttc tcgttttgaa taactttcca
     9721 tatttggcgg agctaaaaag agggaaacct ctaccatgcc cccggggttg gtaaatttta
     9781 gtgcattggc aattacgtta taaagggctt tttcaatttt atccggatca aagtagaaat
     9841 attgttcttc cagttcggtt ttgaatatat accgaatatt tctctgttca gcctggagat
     9901 gaaaaaccga aaagatggct ctaacaaatg agataaactc tttttgctgt aattccagtt
     9961 tcatactacc ggtatcaagt ttacgggcat ccataagctg attgacgagt ctcagcaacc
    10021 ggcgggcgtt ttggagcacc agccgattat agacatctcg tttttcctgg gacaatttat
    10081 tgccgagatg aagcattctt tcgaccggcc cgataatgag agtaagcgga gtccggaact
    10141 cgtgcgatat gtgagtaaaa aatcgtaact taagttgatc caattcatga gtcttttcgg
    10201 cctctaaccg tctcatttca gcctgagccc gattgcgttc gcgcacctgg acagccatac
    10261 gaattaacaa aatggcaccg agaatcaaac cgatatagat aatcaacatc caattagttt
    10321 gccagaaagg cggagtgatt ttaagatgaa tggctgtgcc ggattcattc caaaccccgt
    10381 aattattgga tgctttaacc ctaaaagtat aattgccagg cggcaagtta gtataagtgg
    10441 cgaaccggcg attggcatcg gtgcggcgcc agtcttggtc gaaaccctcc attatataag
    10501 catattgatt cttttgcggg cagctataat cgagagcggc aaactcgaaa gaaaagacat
    10561 tttcgcggta agaaaggatg atattttccg taataaaaat cgaactatct aacttgatct
    10621 ttccctgcgt attcggacta attgtttcat taaaaatttg aaactgggta atcaccactg
    10681 gcggaatgaa cggattgtaa ctaatactgc ccggttgaaa aacattgaat ccatttattc
    10741 caccaaaata aagcataccg ttgctggcgc gtaaaaccga ttcacgataa
//
LOCUS       DAOMJU010000073        10552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6445_length_10552_cov_21.430885, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000073 DAOMJU010000000
VERSION     DAOMJU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6445_length_10552_cov_21.430885"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /locus_tag="PLO58_07350"
     CDS             complement(<1..1948)
                     /locus_tag="PLO58_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO74643.1"
                     /translation="MKKQVIIILILTGLAIRGIASTITGKVTCQMERKPIPDALVILQ
                     KRSDPDFSRTTVSDKTGQFTLTNVEPGVYNIEAVREGYFKNVLFDLKIEADRNYEINI
                     KLLKQEGKSSSDYCFMLGGIEVYSVQKELIPESPVTTRTINTGEIEHMQATSLGDILS
                     LVPGVDKSQNPGLSGRSEIGLRSVTAAGVSMSAVESFGSTIIINDNQISTDANTSGNV
                     YYGSATRGLDLRIIPADNIKSVEVITGIPSVEYGNFASGVIKVETKIGRIAPKFNAKI
                     NPDTKNASFSHGIKLGRSILDYHLNYGYSERDLRKVGDEFQRLHASGNLSGAYLDQKL
                     ETHIGGSFTKLLDNEKPTDVYQMASYDKGFLATGDFNFIYKDSEAKSWKGVFSVNLNN
                     KRNYKSKWVAENYILKYDTTVVDSVTGIAIDTTLIKILPGFIGKMKEIGEEWKIAGKI
                     QRKIALTTKHTKHNLLFGGEVNYEKNTGDGVVIDENFPYYGQYSSHRSYAFDDYPDLT
                     SLSFYAEDVLEGKMLARRYKLMAGLRYDAYSPIGFNFKNMFKEKAFLKSEHGDFLSPR
                     LNLQYSITEDWQMRLGAGRSVKDISLGHIFKAPDYFKYASGDTIIEEVHLQYNPDLQA
                     YSIDKYEASLDWKFSDLIGFSLTGY"
     gene            complement(1963..4743)
                     /locus_tag="PLO58_07355"
     CDS             complement(1963..4743)
                     /locus_tag="PLO58_07355"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQO74644.1"
                     /translation="MKWFRSLAYTIAIILIIFSSLLALDESRVKEFTLNNGLKVITYE
                     MHTAPVIYSQLTYNVGSKYEPFGKTGISHVVEHMMFKGTNRFPKGTISELISANGGIF
                     NAYTSTDITVYYELLPQNKIDLAFDIESERMYKCAFDPQEFESELKVIMEERKQRTEN
                     SAVGLRREEINTLIYKNHPYRNPIIGWMEDIRSINRDDAYKYYRTYYTPNNATLVLTG
                     DFNTDEIMQKVEAYFGKIPRGPEIPAYNFYRVPICGKRTLDFIHPDILSESIHYYFEA
                     PTRFEADGPALYVAGQILGARSSTSRLNKKLKREKQVCESAVAGLSFTKDPSTFNLIT
                     SLMPTAQISEVENLIWKEIDSLATTAVLDYDLQKIKNQIVFNELTGNQHNEKIGGQLA
                     TYDNFYNWRYINQWNQLVMAVTADDIIRVVNKYLKTGNYVACYSHPDTTQKKVGFLAA
                     ESDESEFDLEITEPETIEESEDTTGKYVNPEVRQIYFPKLDEVISPHPIAPLVTTAKL
                     KNGVPVYFLENHEYPTILLIGYIETGRLEEENLHPGIRQMTVGMLSRGTAKRTYEELI
                     EERSFIPYQIDISQSWNKIIFQGYGLSKDVDKILQANFEIVTQPTFPESEIEKVRPSL
                     IKTAKEYKETDRMKAFYAMFEKVFEGHQYALPYAGDPETYRILTSADLKKFHAKYYAP
                     DNLKLLVVGDFNRKEMLSRLNATYGSWKHKSGDKHLPFQTVKPIQGKTVYVFSNPGYK
                     QCRVDIAFCPVEGGIKTDNPDIEALKLLESILCGSSLTSRMGKELRDKQGLSYSIKSN
                     LWIRDHGGYWNIRTELDQANLSKMIHGIFAEIEKVQREGVTDEELEKAQARRIAMLTM
                     NTRTPDEIGAVIFDLIQEKKPLDYFDHSKERILAVTKADIQRVANKYLDTQNYIIAVS
                     GNMAPDALNEFK"
     gene            5291..5361
                     /locus_tag="PLO58_07360"
     tRNA            5291..5361
                     /locus_tag="PLO58_07360"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5323..5325,aa:Gln,seq:ttg)
     gene            5386..6327
                     /locus_tag="PLO58_07365"
     CDS             5386..6327
                     /locus_tag="PLO58_07365"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQO74645.1"
                     /translation="MKNNGIIFCGRSNPQLGYRINEILGIKPGAIKISNFSDGEITVK
                     LEDNIRGKDVFIIQSTNPPAENILELLLIIDAARRASARRITAVLPYYGYARQDRKDQ
                     PRVPISARLILDTIVAAGANRIMAMDLHSTQIQGFVNIPFDHLYAKPIFVEPLRKLIG
                     KRDFSIVTPDVGGIKVARSYAKILGLPITIIDKRRPKPNMSEVMNVIGEKDMKHALII
                     DDMIDTGGSITEAAALLKEYGTETITAVATHGLFSGDCVKRLSDSPIDRVIVTDSVAI
                     PEEKHFAKLQILSAAPMLAEAINRIHNEESISSLFEL"
     gene            6344..7012
                     /locus_tag="PLO58_07370"
     CDS             6344..7012
                     /locus_tag="PLO58_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQO74646.1"
                     /translation="MVDYYKLNAELRSDFQKGVTKRMRRAGLVPAVYYHHTEKPVALS
                     LDLKDLKAALRSSARIYDLNINKKSHKCIIRDVQFDPLTEEIIHADFMGVSLEEMVTV
                     SIPINVTGTAIGVKTFGGILEQHLWELEVKCKVSQIPDRVTIDVTNLNLGDSINAGSL
                     NIEGVEILIPATTSIVSVVKPTGTKAEEEVAKEEVTEEVGAEETAGTQEAKETKETKG
                     KTEK"
     gene            7038..7679
                     /gene="pth"
                     /locus_tag="PLO58_07375"
     CDS             7038..7679
                     /gene="pth"
                     /locus_tag="PLO58_07375"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQO74647.1"
                     /translation="MIRTIIGLGNPGKKYQHNRHNFGQMCLNHIARRHNLAFKPGGGE
                     RAFTYATTVIADRTVYLCQNTTFMNNSGLAVRQILNQFDLQTDEILVVYDDIDLPLGK
                     IRLREKGSAGGHRGIRSIIETLATNQFARLRLGIGPQDEGVPSEDFVLTDFRPAEKEL
                     VEAVLEESYKCILTILNNGIRPAMEYFNRRALHLAPKKNEIQVNSIKKPIILT"
     gene            7691..8038
                     /gene="rpsF"
                     /locus_tag="PLO58_07380"
     CDS             7691..8038
                     /gene="rpsF"
                     /locus_tag="PLO58_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQO74648.1"
                     /translation="MRYYENLFIVNPNIEQEKFTQLIQTVKAEIERLDGNILNLEDWG
                     KRRLAYPIQKHRYGSYVLAQFETENIELVKELESWLRLNQGILAFMTVALDEKPRPKT
                     SQPTDQPVDETIG"
     gene            8077..8313
                     /gene="rpsR"
                     /locus_tag="PLO58_07385"
     CDS             8077..8313
                     /gene="rpsR"
                     /locus_tag="PLO58_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQO74649.1"
                     /translation="MVLLSKRKICKFCENPEEKIDYKNYKVLRRFITEQGKIIPSRIT
                     GTCARHQRELTKAIKRARNIALLPYTYDVTQNYK"
     gene            8335..8778
                     /gene="rplI"
                     /locus_tag="PLO58_07390"
     CDS             8335..8778
                     /gene="rplI"
                     /locus_tag="PLO58_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQO74650.1"
                     /translation="MRVILIKDHENLGKIGDTVEVKDGYARNYLIPQGIALVANSAGM
                     KQMQEQIRLKELRHNKALKKAHELAEKLNTLSLTIPVQVGEEDRVFGAVTSQLIAEQL
                     QEKGYEIDKRNIQLEEPIKALGIFDIPIKIFQDVTAKVKLWVIKA"
     gene            8874..>10552
                     /gene="priA"
                     /locus_tag="PLO58_07395"
     CDS             8874..>10552
                     /gene="priA"
                     /locus_tag="PLO58_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQO74651.1"
                     /translation="MSAKVQYVDVIFPATLEKSFTYQAPSGYDGTLAPGQRVVAPLKS
                     SSAIGFIINLDPIVPANLKIREIQEIIDPQPIFPPELLTFLKKIADYYLTPFGRVLAA
                     AIPAEYRFIKTRTLKASRLDGAQPTEYGDLFAYIAERREVKYSQLKRQFETIYLQKGL
                     MSLKKEGFIAELVEFKPSKYQGHIEKTVRLLNTDEETLSAIARKAPRQAEILNALQSV
                     GEPLKGRDLRRFSPVALNALARKELIAIDTTDRTLSDLWSEFGERRKTITLTEDQYQV
                     FQAISDSIKSAKYNAFLLEGVTGSGKTEIYLEAIRETLAQGRTALVLVPEITLTTHLA
                     ARFRGEFQDQIAIWHSRLTQAQRSAIWGAVLRGEFPVVIGARSAIFMPLPNLGLIIVD
                     EEQDNSFKQKSPEPRYHARDAALLRGVESQATVVLGSATPSLESQYNAIIGKLNKLAL
                     PRRYSKAPTAVIQVVDMKAEIQETGDVQNPFSRLLTAKIKDRLENGQQVLLLQNRRGY
                     SPVLLCPDCGWVPECRNCDIAMTYHKKSATLICHYCNLHETPPAVCPRCRS"
BASE COUNT         2981 a         2309 c         2310 g         2952 t
ORIGIN      
        1 aatagccggt cagggaaaaa cctatcagat cgctgaattt ccaatcgaga gaagcttcat
       61 atttatcaat ggaataagcc tgtaaatccg gattatactg caggtgcact tcttctatga
      121 ttgtatctcc tgaggcatat ttgaaataat ctggagcttt aaaaatgtgc cctaaggaaa
      181 tatccttaac gcttcggccg gcgcccaagc gcatctgcca atcttcagtg attgagtact
      241 gcaaattcag acggggactt agaaaatctc cgtgctctga tttgagaaag gctttttcct
      301 tgaacatatt tttgaaattg aatccaatcg gagagtaagc gtcatagcgt aaaccagcca
      361 tcagtttata gcgccgcgcg agcattttgc cctcgaggac atcctcagca tagaaactca
      421 gggacgtcaa atcgggataa tcatcgaaag cataggagcg atgcgaggaa tattgcccgt
      481 aataggggaa attttcgtca attactacgc cgtccccggt gtttttttca taattgactt
      541 ccccgccgaa taaaagatta tgctttgtat gcttcgttgt caaggcgatt ttacgttgga
      601 tttttccggc aattttccat tcttcgccga tttctttcat cttgccgata aatcccggca
      661 aaattttaat cagggttgta tcaatcgcga ttcccgtgac cgaatccacg acagttgtat
      721 catatttaag aatataattt tccgccaccc atttggattt atagtttctt ttattattaa
      781 gattaactga gaagacgcct ttccaacttt tagcctcgct atctttataa ataaaattga
      841 aatcgccggt tgccagaaat cctttatcat aagaagccat ctgataaacg tcagtgggtt
      901 tttcattatc gagcaacttg gtaaaacttc ccccgatatg ggtttcgagt ttttgatcta
      961 agtatgcacc ggataggtta ccgctggcgt gtaaacgctg aaattcatcg ccaactttgc
     1021 gcaaatcgcg ttcgctatac ccgtaattaa ggtgataatc cagaatagag cgaccgagct
     1081 taatgccatg tgagaagcta gcgttctttg tatccggatt gattttagca ttgaatttag
     1141 gagcaattcg gccgatcttg gtttcaactt taataacgcc gctggcaaaa ttgccgtatt
     1201 caaccgaggg gatgccggta attacttcaa ccgatttaat attatcagcc ggaatgatac
     1261 gcagatccag gcctctggtg gcactcccgt aatagacatt gccggaagtg ttagcgtcgg
     1321 ttgagatttg attatcatta ataattattg ttgatccaaa agactcgacg gcactcattg
     1381 atacaccagc ggcggtcacc gaacgcaagc cgatttcact gcggcctgag agacccggat
     1441 tctgcgattt gtccacgccg ggaacaagtg agagaatatc gccgaggctg gtagcttgca
     1501 tatgttcgat ttcaccagta ttgatagtgc gagtggtaac cggcgactca gggattaatt
     1561 ctttttgcac tgaatagact tcgataccgc ccagcatgaa acaataatcg ctggaggatt
     1621 tgccctcctg cttgagcagt ttgatattaa tctcgtaatt tcggtcggct tcgattttta
     1681 gatcaaataa cacatttttg aaatatccct cgcgcacagc ttcgatatta taaacacctg
     1741 gttcaacatt tgtgagcgtg aattgaccgg tcttatcgga aaccgttgta cgtgaaaaat
     1801 ccggatcgga acgtttttgc aggattacta acgcatccgg aatcggtttc ctctccatct
     1861 ggcaagtgac tttccccgtt atagttgagg caattccccg aatcgctaaa ccagtcagga
     1921 ttagaattat tatcacctgc tttttcatat tcttttagcg attcatttga attcgtttag
     1981 ggcgtcgggc gccatattcc cagaaactgc gatgatgtaa ttttgagtat cgagatactt
     2041 attggctacc cgctgtatat cagcttttgt aacggcaaga atgcgctctt tactatgatc
     2101 aaagtaatca agcggcttct tctcctgaat taaatcgaaa atgaccgcac caatttcgtc
     2161 cggtgtccga gtattcatcg ttagcatagc aattcggcgg gcttgggctt tttccagttc
     2221 ttcatcagta actccctccc tttgaacctt ctcaatttca gcgaaaatgc catgaatcat
     2281 tttactaaga ttggcttggt ctaattcagt acgaatattc caataaccgc cgtgatcgcg
     2341 aatccagaga ttgcttttga tactataact taagccttgt ttatcgcgta attcttttcc
     2401 catgcgggag gttagcgaac ttccgcaaag gattgattcg agtagtttaa gagcttcaat
     2461 atcgggatta tctgttttga tgccaccttc taccgggcaa aaggcgatat ctactcgaca
     2521 ttgtttgtat cccggattgc taaaaacgta aacggtttta ccctggatcg gcttaaccgt
     2581 ctggaacggc aaatgcttat ctccggattt atgtttccaa ctgccataag tggcatttaa
     2641 gcgcgaaagc atttctttgc gattgaaatc gccaactacc aataatttca gattgtcagg
     2701 tgcataatat ttggcgtgaa atttttttaa atcagcactg gtcaaaattc tgtaggtttc
     2761 cggatctccg gcgtagggta aagcatactg gtgcccttca aaaacttttt cgaacatagc
     2821 gtaaaaggct ttcatacgat cggtttcttt atattccttc gcggttttaa ttaacgacgg
     2881 gcgaactttt tcaatttccg attccggaaa agtaggctga gttacaatct cgaaattagc
     2941 ctgtagaatt ttatctacat cttttgagag cccataaccc tgaaaaataa tcttattcca
     3001 gctctgacta atatcaatct ggtagggtat gaacgagcgc tcttcgatca attcttcata
     3061 agtgcgttta gcggtgcccc gcgaaagcat acctaccgtc atctggcgaa taccaggatg
     3121 caaattttcc tcttcgagcc gccctgtttc gatatatccg attagcaaaa tagttggata
     3181 ttcatgattt tctaaaaaat aaaccggcac gccgtttttt agcttagctg tggtaaccaa
     3241 cggtgcgatc ggatgcggtg aaatcacttc atctaactta ggaaaatata tctgacgcac
     3301 ttccggattg acatatttac cggtagtatc ttcagattct tctatcgttt ccggttcggt
     3361 tatttccaga tcaaattcac tttcgtcact ttctgcggct aagaatccga ctttcttttg
     3421 ggtagtatct gggtgggagt aacaggcgac ataattgccg gttttcaaat atttattaac
     3481 gacccgtata atgtcatcag cggttaccgc catgacgagc tgattccatt gatttatata
     3541 acgccaatta taaaaattat cataagttgc aagttgccca ccgatttttt cattatgctg
     3601 attaccagtt agttcattaa agacgatttg atttttaatt ttttgaagat cataatctaa
     3661 gaccgccgtc gtcgccaaac tgtcaatttc cttccagatt agattctcaa cttcgctgat
     3721 ttgggcagtt ggcatcaagc tggtgattag attaaaagtc gaggggtctt ttgtaaatga
     3781 aaggccggct accgccgatt cacaaacctg cttctcgcgt ttcagctttt tattaagtcg
     3841 cgaagtcgaa ctgcgggcgc ccagaatctg cccggctacg tataaagctg gtccatcagc
     3901 ttcaaaacgg gttggcgctt caaaataata atggattgat tcgcttaaaa tatcgggatg
     3961 aatgaaatcg agcgttctct taccgcaaat tggaacacga taaaaattat acgcgggaat
     4021 ctccggaccg cgcggaattt taccaaaata ggcttcgact ttttgcataa tctcgtccgt
     4081 attgaagtca ccggtgagaa caagcgtggc attgttgggg gtatagtagg tacgatagta
     4141 tttataggcg tcatcacgat taatactgcg aatatcttcc atccagccga taatcggatt
     4201 gcggtaaggg tgatttttgt agatgagagt attaatctcc tctcgacgca atccaaccgc
     4261 gctattctcg gtgcgttgtt tgcgttcttc cattataacc ttgagctccg attcgaattc
     4321 ttgagggtca aaagcacatt tatacatccg ttcggactcg atgtcgaagg ctaaatcgat
     4381 tttattttgg ggaagtaatt catagtaaac cgtgatgtct gtggaggtat aagcattgaa
     4441 tataccgcca ttagcactaa tcagttcgga aatagtacct ttcggaaaac gattagtgcc
     4501 tttaaacatc atatgctcga cgacgtgact tatcccggtc ttgccaaacg gttcatattt
     4561 cgatccgaca ttgtaagtca attgggaata aatgactggt gcggtgtgca tttcatacgt
     4621 gatgactttc aatccgttat ttagagtaaa ttccttgacc cgtgattcat cgagagccag
     4681 gagactcgag aaaataatta atattattgc aattgtatag gctaaactac gaaaccattt
     4741 cattctaaat tgttcctttt ttgatttaaa tgttcactaa tattctaaaa aactcaacga
     4801 atataaaata atttctacat aaaatcaatg aagtcaagga agactcgggg gatatgattt
     4861 gctatatttc ttagagaaaa attgatagcc agaattcagc aatagattgt tataagggca
     4921 aaataaaatc ggaaattagt ggataaagaa ttgattcaat tctggtatga aacactaaaa
     4981 aatatggaaa ctatgagtgt ccccctaaaa ttttaattgt ttcctttgtc agataattat
     5041 cttggcaaaa agataatatt tatctaaatt tccgtctctt ttgaaattag atgttggctg
     5101 tttgaaataa taagtgagtc tgttgttttt ccagcatatc cgggagctga agcgtaatcg
     5161 gcttgtcggg gacgctcgac tgtgtatgga atctttgcca gcagagatag tggccgccgc
     5221 agtataatgt cggtaaatta ccacacacag atcatcatac ccttcccact tgaaaaacaa
     5281 cctaaacaat tcggggatcg tccaatggca ggaccacggc ctttggagtc gttaatctag
     5341 gttcgaatcc tggtccccga gcaagttttt aaaatgagcc cgaatatgaa aaataatgga
     5401 attatttttt gtggaaggtc caatccccag ttgggatacc ggatcaatga aattctcggg
     5461 ataaaacccg gagcaattaa aattagtaat tttagcgacg gcgagatcac cgtcaaattg
     5521 gaagacaata tccgcggaaa agatgtcttc attattcaat ctaccaatcc accggctgaa
     5581 aatattttag aacttctgtt gattattgac gccgcccggc gcgcgtctgc ccgtagaatt
     5641 accgccgttc tcccctatta tggttacgcc cggcaggatc gcaaggacca gccgcgggtg
     5701 ccgatttccg cccgtttaat tttagatacg atcgtcgccg ccggagccaa ccgaattatg
     5761 gctatggatt tacattcgac ccagattcaa ggcttcgtta atattccctt tgatcacctg
     5821 tatgccaagc ccattttcgt cgagcccttg cggaagctga tcggcaaacg cgatttctcg
     5881 atcgttaccc ccgatgtggg cggaatcaaa gttgctcgtt cttacgctaa aatcctcggt
     5941 ttgcctataa ccattattga taagcgccgc ccgaagccga atatgtcgga agttatgaac
     6001 gtgatcggtg aaaaagatat gaaacatgcg ttaataattg acgatatgat tgatactgga
     6061 ggcagtatta ctgaagccgc cgctttgtta aaagagtatg gtacggaaac aatcactgcc
     6121 gtcgccactc atggtctttt ttccggcgat tgtgttaaac gcctgagcga ctcgcctatt
     6181 gatcgcgtca tcgtcaccga ttccgtagcg attccggaag aaaagcattt tgcgaaattg
     6241 caaattctct cagccgcgcc gatgttggcg gaggcaatca atagaattca taatgaagaa
     6301 tcaattagtt ccctatttga attataagta aaggagtttt tagatggttg actattacaa
     6361 attgaatgcc gaattacgtt ccgatttcca gaaaggagtc accaagcgca tgcgtcgcgc
     6421 aggtctggtt ccggcagttt attaccatca taccgaaaag ccggtagcat tatctcttga
     6481 tttgaaagac cttaaggcgg ctctacgctc cagcgcccgt atttatgacc ttaatattaa
     6541 taaaaaaagt cacaaatgca ttatacgcga tgttcagttc gacccgctta ccgaagagat
     6601 tatccacgcc gactttatgg gcgtcagcct ggaagagatg gtgaccgtca gcatcccgat
     6661 taatgtcacc ggtaccgcca ttggcgtaaa aacttttggt ggaattctgg agcaacatct
     6721 ttgggaattg gaggtcaaat gtaaagtgtc ccagattccc gatcgcgtta cgattgacgt
     6781 gactaattta aacctcggcg attcaatcaa cgccggtagc cttaatatcg agggcgtaga
     6841 aattctaata cccgctacga cttcgattgt ctcagttgtt aaaccgacag gtaccaaagc
     6901 cgaagaagaa gtcgctaaag aagaagtcac cgaagaagtc ggcgccgaag aaacggctgg
     6961 cactcaggaa gcgaaagaaa ctaaagagac caaaggtaaa actgagaaat aatgaacagc
     7021 tcaatgaacg gctacaaatg atcaggacaa tcatcggttt gggaaatccc gggaaaaaat
     7081 atcagcacaa tcggcataat tttggccaaa tgtgtctaaa ccacattgct aggcggcata
     7141 atctggcatt taaaccagga ggcggcgagc gtgcctttac ttacgccacg actgtaattg
     7201 ctgatcggac agtctatctt tgccagaaca ccacttttat gaataatagc ggactagctg
     7261 ttcgtcagat tctaaaccaa ttcgacctgc agactgatga aatactggta gtttacgacg
     7321 atattgactt gccactcggt aaaatccgtt tgcgcgagaa gggctccgcc ggagggcatc
     7381 gcggtatacg ctctataatc gagacgctcg ccaccaacca gtttgcccgc ttgcggctgg
     7441 gaattggtcc gcaagatgaa ggcgttccat cggaagattt tgtcctgacg gattttcgtc
     7501 cagcggaaaa agaactggtt gaagccgtac tggaagaaag ctataagtgt atcctgacca
     7561 ttctaaacaa tggcattcga ccggcgatgg aatatttcaa tcgccgcgct ctgcaccttg
     7621 ctcccaaaaa aaatgaaatt caggtaaatt caattaaaaa accaatcata ttaacataag
     7681 gagttcggac ttgaggtact atgaaaattt atttatcgta aatcccaata tcgaacagga
     7741 aaaattcacg caattgattc agactgtcaa agccgaaatc gagcgtttag atggtaacat
     7801 tttaaatctg gaagactggg gcaagcgccg tctggcttat ccaatccaga aacatcgtta
     7861 cggctcgtat gtgctggctc aatttgaaac cgaaaacata gaactggtca aggagctgga
     7921 aagctggcta cgtttgaatc agggcatttt ggcttttatg acagtcgcgc tggatgaaaa
     7981 accgcgtcct aagacttctc agccaaccga ccagcccgtg gacgaaacta ttggatgaac
     8041 gaagattgga tgatttaaaa aagaaaggaa aaaaatatgg tattattgag caaacgcaaa
     8101 atttgcaaat tttgtgagaa tcccgaggaa aagatcgact ataagaatta caaggtattg
     8161 agaaggttca taaccgaaca ggggaaaatt attcccagcc ggatcaccgg cacctgtgcc
     8221 cgccatcagc gcgaattgac gaaagcgatt aaacgagcgc gcaatattgc tttactgcct
     8281 tatacctacg acgtaaccca aaattataaa tagtatcagc aacaggaggt atccgtgcgc
     8341 gttattttaa tcaaagatca tgaaaacttg ggcaagattg gggataccgt tgaagttaaa
     8401 gacggctacg cccgcaatta cctcattccg caaggcatcg cgttggttgc caatagcgcc
     8461 ggaatgaaac aaatgcagga acaaatccgc ctaaaggaac tgcgccataa taaggctctt
     8521 aagaaagccc acgagttggc ggaaaaactc aacaccctgt cgctaacgat ccccgttcag
     8581 gttggagaag aagatcgcgt cttcggcgcc gtgacttccc agctgattgc cgaacagttg
     8641 caggaaaagg gctatgaaat tgacaagaga aatatacaac tcgaagagcc gatcaaagcc
     8701 ctcggcattt tcgatattcc cataaaaatc ttccaggatg tgaccgccaa ggtcaaactc
     8761 tgggtgatca aagcctgata tatttttcaa atatttatta atccaaagac cgaatcgttc
     8821 cggtttaatc ttgtcttttt tatttctttt ggataatctt tatagcgaaa attatgtctg
     8881 ccaaagttca atatgtagat gtcatttttc cggcgactct cgagaagagt ttcacctacc
     8941 aggcgccgag cggttatgac ggaacacttg ctcccggtca gcgggtagta gcgccgctaa
     9001 aatccagctc ggcgatcggt tttataatta atctcgaccc gatcgtacca gccaatttga
     9061 agattcgcga gattcaggaa atcattgatc cccaaccgat ttttcctccg gaattgctga
     9121 cctttttgaa aaaaatagcg gactattacc tcactccctt tggccgggtt ttagccgcag
     9181 ctattcccgc ggaatatcgc tttattaaga cccgcacctt aaaagcaagt cgtcttgacg
     9241 gtgcgcaacc gactgaatat ggcgatcttt ttgcttatat cgccgaacgg cgtgaggtaa
     9301 aatacagcca gcttaaacgc caatttgaga cgatttacct gcaaaaagga ctgatgtcgt
     9361 taaaaaaaga aggtttcatc gccgagctag tggaatttaa accgtcaaaa taccagggac
     9421 atatcgaaaa gaccgtaaga ttattgaaca ctgacgaaga aaccttgagt gctattgcgc
     9481 gcaaagcccc tcgacaagcg gaaatcctga atgctttaca gagcgtcggg gagccactga
     9541 aaggcagaga tttgcggcgt ttttcaccgg tagccctcaa tgctttagcc cgcaaagaac
     9601 taattgcgat tgatacaacc gaccgaaccc tttctgattt atggagtgaa ttcggtgaac
     9661 ggcgcaagac gattactctt actgaagatc agtatcaagt ttttcaggca atttccgatt
     9721 cgatcaaaag cgctaaatat aacgcctttt tgctggaagg cgtaaccggt agcggcaaga
     9781 ccgaaatcta cctggaggca attcgagaaa ccttagctca agggcgcacg gcgctcgttt
     9841 tggtgccaga aataactttg acaacgcacc ttgccgcccg ttttcgtggg gaatttcagg
     9901 atcagattgc catctggcat agccgactga cccaagccca gcgcagtgct atctggggtg
     9961 cggttttgcg cggtgaattt ccggttgtca ttggcgcgcg cagtgccatt tttatgcctc
    10021 tgcccaatct cggattgatt atcgtcgatg aagagcagga taattcgttt aagcaaaaaa
    10081 gtcccgaacc gcgctatcat gcccgagatg cggctttgtt gcgcggtgtc gaaagccagg
    10141 cgaccgtcgt gctaggttca gctactccca gcctcgaaag tcagtacaat gccattatcg
    10201 gtaaattgaa taagctggct ttgccccgtc gttattccaa ggctccgact gctgtaatcc
    10261 aggtagttga tatgaaagcg gaaattcaag aaaccggtga tgttcagaat ccattttcac
    10321 gtttgttgac tgccaagatt aaagaccgac tggaaaatgg tcagcaggtg cttctcctcc
    10381 agaatcggcg gggttactcg ccggtcttgt tatgtccgga ctgcggctgg gttccggaat
    10441 gtcgcaattg cgatatcgct atgacctatc ataaaaaatc cgcgacgtta atttgccact
    10501 actgtaattt acatgaaaca ccaccggcgg tttgtccgcg ctgtagaagt ag
//
LOCUS       DAOMJU010000074        10376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6589_length_10376_cov_23.796822, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000074 DAOMJU010000000
VERSION     DAOMJU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6589_length_10376_cov_23.796822"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1008
                     /gene="aroF"
                     /locus_tag="PLO58_07400"
     CDS             157..1008
                     /gene="aroF"
                     /locus_tag="PLO58_07400"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQO74652.1"
                     /translation="MGNSENLKLTSIESHLNKTTIKIGDVTIGQDFVVIAGPCAVESE
                     EQLMQTAEAVKAAGANMLRGGAFKPRTSPYDFQGLGLKALKLLEKAKKKYGLPIVTEV
                     LDSRDVTWICEYVDILQIGARNMQNFALLKEVGKSSKPVLLKRGMYSTLKEWLNCAEY
                     ILSEGNPNVILCERGIRTFETYTRNTLDLSIVPAVREVTHLPVIVDPSHATGRLGLIK
                     PMSLAAIAAGVDGLIIEVHYKPETALSDRDQQLNPEQFKVLMRDVLSLKNFMQTLSNS
                     NNASHSN"
     gene            989..2026
                     /gene="aroB"
                     /locus_tag="PLO58_07405"
     CDS             989..2026
                     /gene="aroB"
                     /locus_tag="PLO58_07405"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQO74653.1"
                     /translation="MPAIQIKTAGKTSTIITCPSIREVFRLIEGRPVVIITDQNLDRS
                     YGDLFPEFPKIVLSNGESSKSLETVRQIYEQLGTAEVDRDYTILGIGGGVVCDIAGFV
                     ASTYLRGLDFGFVATSLLAQVDASIGGKNGVNFAGAKNIIGTINQPRFVLCPLDVLET
                     LPRAQFINGTAEIIKTGAIGDADLLEMLATQQAQFINLVPDVTEQIIFRTLNFKAGIV
                     MRDEREQGERRKLNFGHTIGHAIEIASGILHGQAVAIGMNMAAEISVRLGLLARSEKE
                     RLEKILTAYELPTATSLPANELLPLIFKDKKRQGEHLRFVALKKLGSAEVINLSFNQI
                     KEFLDDLCRLG"
     gene            2004..2648
                     /locus_tag="PLO58_07410"
     CDS             2004..2648
                     /locus_tag="PLO58_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HQO74654.1"
                     /translation="MICVALAELNFPEIMAILKTEEMVEIRIDLLNLTETQIIAIFRS
                     HPNLVATCRKGRYSDEERQALLALAIRNGAAYVDLECDSPEDWRRSLTQLAREHHTRL
                     IISYHNFELTPGLAELHLLLRNCEALPSDIVKIVTYCQTESDAERLLSLYSLNTKPLI
                     AFGMGEAGRKSRLKALRLGAPFIYAAAETGQPTAPGQMTKNEILELLAEESANV"
     gene            2641..>3401
                     /locus_tag="PLO58_07415"
     CDS             2641..>3401
                     /locus_tag="PLO58_07415"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQO74655.1"
                     /translation="MSEKRFAIIGHPILHSKSPQIHNHWFRENNLSANYSRIIANDVY
                     VALGMGYKLGLTGANVTAPFKEKVIPSLVYLSPEAAAVGAVNTLIYNGPNISGYNTDV
                     TGVIRAFQINNVELKHKSLLVIGAGGAARAIVSALKPSGAEITIVNRPDEMALNLARS
                     FQVKSVPFSEIQTAVRTAEIIVTALPPGVRVVEPEWLNSRQIIMDANYKNSEMEPLAQ
                     AVGARFISGIVWLTEQARPAFELFTGIQPASDIPPA"
     assembly_gap    3402..3501
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3502..3795
                     /locus_tag="PLO58_07420"
     CDS             <3502..3795
                     /locus_tag="PLO58_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQO74656.1"
                     /translation="IAGGGGIILSEINRALICQKALSIWLFARPLQIWPRIEQAGRPL
                     LKSDDPLATAEQIFRTRSDLYFNTADGILDTEIATPETCTDLLYAEISQIFHD"
     gene            3764..5014
                     /gene="aroA"
                     /locus_tag="PLO58_07425"
     CDS             3764..5014
                     /gene="aroA"
                     /locus_tag="PLO58_07425"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQO74657.1"
                     /translation="MQKSVKFSTIDGTVAAPASKSIVQRVIAMALLAEGTSRFGNVTL
                     CDDSQAAIEIARALGAEIELTERNLTLKGGFKPRKKVLDCGESGLCSRLFAAVAALSP
                     QELILTGRPALRKRPADMIPEPLQKLGARCESDNGHLPLKISGPLKGGKVKIDGSVSS
                     QFLTGLLIALPVVAADSEISVDNLKSKPYIDLTIEILEKFGIHIENYNYCRFIIPGGQ
                     KYQATEFNVEGDFSGAAFLLTAGALRGRITVTGLNPKSQQADRAILDLLQQCGARVKI
                     SPDEISVTQSELQAFTCDANHYPDLFPPLVALAAGCEGVSQIAGVERLLYKESDRAAV
                     LKTEFGKLGIDINIEGDAMLIKGGKIQGGLTEAHNDHRIAMALAIAGLCSEKPVVIAG
                     AECVTKSYPTFFDDLKSVGGNIDE"
     gene            5007..6008
                     /locus_tag="PLO58_07430"
     CDS             5007..6008
                     /locus_tag="PLO58_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQO74658.1"
                     /translation="MNSFGRLFRLTIFGESHGPAVGVIIDGCPPGLEIKESDLTADLD
                     RRRSGAPGTTARQESDQPRFLSGVWNNHTTGAPLTIVFENSAQRSADYAEKPTVYRPG
                     HADFTADRKFRGWNDPRGGGHFSGRMTVGLVAAGVLAKKIIAPVEVSASLIEVGGQAD
                     YSELLEQARRDGDSLGGIIECRAQNLPVGLGEPFFDSVESVLAHAIFAIPGIKGIEFG
                     SGFAAARMRGSEFNDCFIAPNGKTATNHSGGINGGITNGNELVFRIVARPTASIARSQ
                     QTLDFDSGKMTDLSISGRHDVCFALRLPVIVETLTACCLTDLLLIQKSWQLNEENNL"
     gene            6005..7060
                     /locus_tag="PLO58_07435"
     CDS             6005..7060
                     /locus_tag="PLO58_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HQO74659.1"
                     /translation="MNLDEIRSQIDLIDGKIIKLLNDRLSQAIMAKKFKTNIEDPQRE
                     QEVFNNLELKTYDLVTPEFARRLYAIIIEEGKRLQSQDLPVIAFQGEHGAYSEMAGKA
                     WNPQLVSIPCREFAELFEGVSDGSYDYGIIPIENTLGGVVGPVNELLISTKLNVVGAI
                     EMPIRHCLLAVRNSDYRELRSAYSHPQALAQCKHFLERNKLDPIPYYDTAGAARMVAE
                     KGLKGIAAIASPLAAQLYNLEIIKEQIEDSPLNTTRFLILAHEPFTGKGEKCSIIFST
                     AHKAGTLFKVLEIFAKAKINLTRIESVPKSPGQYAFFLDFIGSKDDPKVEKVLEEVAQ
                     STSDFRLMGCYLERILA"
     gene            7057..7842
                     /locus_tag="PLO58_07440"
     CDS             7057..7842
                     /locus_tag="PLO58_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQO74660.1"
                     /translation="MKIFILGTGRMGAWLVEEFCHQHQVAVYDRDLNKLKCFDNVERF
                     SDLNEVTSFQPELAINAVSPQNTVSAFEELLPLIPANCMLSDIASVKAGVAEFYRKIG
                     RRFVSSHPMFGPTFANIRDLRHENAIIISESDPAGKAFFRHFFENLQLNIFEYTFEEH
                     DQTTAYSLGTPFVSTLTFAANLKMQKVPGTTFRKHLEIARGLLSEDDYLLSEVLFNPY
                     TLQQIELINSRLSFLTHIIRGRDYEEMKRFLDNLRKNISALKE"
     gene            complement(7971..8561)
                     /locus_tag="PLO58_07445"
     CDS             complement(7971..8561)
                     /locus_tag="PLO58_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74661.1"
                     /translation="MNSLDSPLKIQTFLDETPYSTEPIYRSPLSVLHDRRAHCFDGAL
                     FAAASLRRLGYPPLIVEMLPNERDDDHLLAVYKRSNHWGAVAKSNFVGLRYREPIFRT
                     LRELILSYFEDYFNIVGEKTLRGYTLPLNLTICDRWNWMIDDEHLDEIANHLDRQRRV
                     NLLTPEMEQNLAPVDQRMLKAGLLGSNPGGLYKPDK"
     gene            complement(8817..9236)
                     /gene="rnk"
                     /locus_tag="PLO58_07450"
     CDS             complement(8817..9236)
                     /gene="rnk"
                     /locus_tag="PLO58_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HQO74662.1"
                     /translation="MTKEREIYITSQDLKRLKKELSMAGISYFHGRGDLQLLAKELDR
                     AKIIEASEIPEKIVTMNTRLIFRDLDDDSTMEVTIVFPIDANINKGKISVFSPIGTAL
                     LGYSEGDTIEWKVPAGITRIRIEKVLYQPEASGEFNL"
     gene            complement(9265..10125)
                     /locus_tag="PLO58_07455"
     CDS             complement(9265..10125)
                     /locus_tag="PLO58_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HQO74663.1"
                     /translation="MRIISKNGTVNLLYQTLLLTSGSILCALAVKALILPHGLLARGM
                     TGFALIIYYIWPVIPLGLLYCLINIPVFLLGWRFVGRRFIKYSLLGMVIYSATLSLIN
                     CEINLSDPLLAVIIAGALSGAGTALILRSYGSSGGSDILCVILNKLFSITLGTGAILI
                     NAVVLILSAFIFPIEKVFYTLVFIFVSGLFTNKIFHGLANRRTAIIISDQWEIIVEKL
                     NAHRIGVTLIKGQGGYRGAGCTLLYSVIPARSVSLLKRLVTEVDAQAFIAIMEADDVT
                     GVEIGNQPHW"
BASE COUNT         2803 a         2382 c         2362 g         2729 t
ORIGIN      
        1 aaagactgat atgaataaca aaagatactt tttttactat ttttatttta aggaaacaca
       61 ccggaagggg taaccttact tttaaatcaa tttattaaaa accccttccg ttgagaaggg
      121 gtttttgtta caggcagact tgaaaaggag aaataaatgg gtaattctga aaatctaaaa
      181 ctgacttcca tcgagagtca tttgaataag actactatca agatcggtga tgtaaccatc
      241 ggacaggatt tcgtcgttat cgccggacct tgcgccgtcg aatcggaaga gcaattaatg
      301 caaaccgctg aggcggttaa agccgccgga gctaatatgt tgcgcggtgg cgctttcaaa
      361 ccgcgtactt cgccctacga tttccaaggg ctcggtttaa aggctttaaa attattagaa
      421 aaagctaaaa agaaatatgg attgccgatt gttaccgaag tgttggattc gcgcgatgtc
      481 acctggattt gcgaatatgt tgacattctc cagattggtg ctcgcaatat gcagaatttt
      541 gcgcttttga aagaagtggg caagagttcc aagccggttt tgctgaaacg cgggatgtat
      601 tcgaccttga aagaatggct gaactgcgcc gagtatattc tttccgaagg taatccgaat
      661 gtgattttgt gcgaacgcgg tattcgtacc tttgaaactt acacccgaaa tacgctcgac
      721 ctgagcattg ttccggctgt ccgagaagtg actcacctac cagttatcgt tgatccgtcg
      781 catgccacgg gacgattagg actgatcaag ccgatgtctt tagccgcgat agctgccggt
      841 gtggacggat taatcatcga agttcattat aaaccggaaa ccgctcttag cgatcgcgac
      901 cagcaactga atccggaaca attcaaagtg cttatgcgcg atgtattgag tctgaaaaat
      961 ttcatgcaaa ctctaagtaa ttcgaacaat gccagccatt caaattaaaa ccgctggcaa
     1021 aacttccacg attataactt gtccttcaat tcgggaagtc ttccgattga tcgaagggcg
     1081 accggtcgta ataataaccg accaaaatct cgatcgttcc tacggcgatc tgtttcctga
     1141 attccccaaa atcgttttgt cgaacggcga gagctccaag agtcttgaga ctgtccggca
     1201 gatttatgaa cagttaggca ctgccgaagt ggaccgcgat tatacaattc tgggaatcgg
     1261 tggcggcgtg gtttgcgaca tcgccgggtt cgttgccagc acttacctgc gcgggttgga
     1321 tttcggattc gtggcgacat ctctgctggc tcaagttgat gccagtatcg gcggtaaaaa
     1381 cggtgtcaat tttgccggtg caaaaaacat tatcggcacg atcaatcagc cgcgttttgt
     1441 gctttgtccg ctggatgtgc tggagacctt accgcgcgcg cagtttatca atggcacggc
     1501 tgaaattatc aaaaccggtg ccatcggcga cgccgattta ttggaaatgt tggcaaccca
     1561 acaagcccaa ttcatcaacc tcgtgcctga tgtgactgag caaatcattt tccgcacgct
     1621 gaatttcaaa gccggcatcg tgatgcgtga cgaacgggaa caaggtgagc gtcgcaaact
     1681 taactttggt cacacgatcg ggcacgctat tgaaattgca tcaggcattc tgcacggcca
     1741 ggcggtagcg atcggaatga atatggccgc ggaaatctcg gtgcgcctgg gtttattggc
     1801 acgtagtgaa aaagaacgct tggagaaaat attaaccgct tacgaattgc cgaccgctac
     1861 ctcattaccg gctaatgagc tcctgccgtt aattttcaaa gataaaaaac gtcagggcga
     1921 gcacctgcgt tttgtagcct taaaaaaatt aggctcagct gaagtaatta atctctcatt
     1981 taatcaaatt aaggaatttc tcgatgatct gtgtcgcctt ggctgaactc aatttcccgg
     2041 aaataatggc cattttaaaa acagaagaaa tggtagaaat ccggattgat ttgctcaatt
     2101 tgactgaaac ccagataatc gcaattttcc gaagccaccc gaatctggtt gcaacttgtc
     2161 gcaaaggccg ctattccgac gaagagcgtc aggctttact ggcgcttgcc attcggaacg
     2221 gggcggctta cgttgatctc gaatgcgatt ctccagaaga ctggcgcaga tcgctgaccc
     2281 aattagcgcg cgaacatcat acccgcctta ttatctcgta tcataatttc gaactgacgc
     2341 cgggtttggc ggaattacat ctgcttttgc gaaattgtga ggcattacct tctgatattg
     2401 tcaagattgt aacatattgt caaacggaaa gcgatgcgga acgtttgtta agtttatatt
     2461 cgctaaacac caagccgctg attgctttcg ggatgggcga agccggtcga aagtcgcgcc
     2521 ttaaagcgct ccgactcgga gcacctttca tttacgccgc ggctgaaacc ggtcaaccta
     2581 ctgcgcccgg acaaatgacg aaaaatgaaa ttctagaact gcttgctgag gagtcggcga
     2641 atgtctgaaa aacgctttgc cattatcggc catccaattc tgcacagcaa aagcccccag
     2701 attcataatc actggtttcg tgaaaataat ttgtccgcca attatagccg gataatcgcc
     2761 aatgacgtat atgttgcgct cggaatggga tacaagctcg gtcttaccgg cgccaacgtc
     2821 accgcgccgt tcaaggaaaa agtcataccg tcattagttt acctctcgcc agaagctgcc
     2881 gcggttggag ccgtcaatac tctgatctat aatggtccta atattagcgg atataatact
     2941 gatgttaccg gggttattcg tgcttttcaa attaataatg ttgaattaaa gcacaagtct
     3001 ctcctcgtaa tcggggcggg tggtgccgcg cgggcgatcg tcagcgccct caaaccttcc
     3061 ggcgccgaga ttaccattgt gaaccgtccg gatgaaatgg ctttgaatct ggcgcgcagt
     3121 tttcaagtta aatctgtgcc cttcagtgaa attcaaactg ccgttcgaac ggccgaaata
     3181 atcgtgacgg cgcttccgcc gggagtgcgc gtggtcgaac cagaatggct caattcccgt
     3241 cagataataa tggacgccaa ttataaaaat tctgaaatgg agccgctggc gcaagcagtt
     3301 ggcgctcggt ttatttccgg aatagtctgg ctaaccgagc aagcccgtcc agcgttcgaa
     3361 ttattcactg gtattcaacc cgcttcggac attccacctg cnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn natcgccgga ggtggaggaa tcattttaag cgagataaat
     3541 cgggcgttga tttgccaaaa agcactgagt atctggctct ttgcccgacc gctccaaatc
     3601 tggccacgaa ttgaacaagc cggtcgtcca ctactaaaaa gcgatgaccc attagccact
     3661 gctgaacaaa ttttcaggac gcgctccgat ttatatttca acacggctga cggcatttta
     3721 gacactgaaa ttgccacgcc ggaaacctgt acggatttac tctatgcaga aatcagtcaa
     3781 attttccacg attgacggta ccgtcgccgc tccggcttct aagagcatcg ttcagcgcgt
     3841 tattgcgatg gctttgctgg cggaaggtac ttcccgcttc ggcaatgtta cgctttgcga
     3901 tgacagtcag gcggctatcg aaatcgcccg agcgctcggt gcagaaatag aattgactga
     3961 gcgtaatctt accctaaaag gtggtttcaa gccacgtaaa aaagtactgg attgcggcga
     4021 atcgggattg tgttcacgct tgtttgcggc ggtagcggcg ctcagcccac aggagttgat
     4081 tctcactggt cgtccggcac ttcgcaaacg tccggcagat atgattcccg aaccgcttca
     4141 gaaactcgga gcccgatgcg aatccgacaa tggtcattta ccattaaaga tcagcggtcc
     4201 gctaaaaggt ggcaaagtaa agattgacgg ttccgtaagt tcgcagtttt taaccggttt
     4261 actgatcgct ttgccggttg tcgccgccga ttctgaaatc agcgttgata atctaaaaag
     4321 caaaccatat attgacttaa ccattgaaat tttggaaaaa tttggtatcc acatcgaaaa
     4381 ttataattac tgccgtttca ttattccggg cggtcagaaa tatcaagcga ccgaattcaa
     4441 tgtcgaaggt gacttcagtg gtgcggcttt tttattgacc gctggcgctt tgcgggggcg
     4501 gattaccgtg acaggactta atcccaagtc ccagcaggct gatcgagcaa ttttggattt
     4561 actacaacaa tgcggtgcgc gggtaaaaat cagcccggac gagattagcg tcactcaatc
     4621 cgaattgcaa gcgttcactt gcgatgcaaa ccattatccg gatctttttc caccgttggt
     4681 ggctctggcg gcagggtgtg aaggtgtcag ccaaatcgcg ggcgttgaac gcttacttta
     4741 caaagaaagt gatcgagcgg cggttttgaa gacggagttc gggaagcttg gaattgatat
     4801 taatatagaa ggcgacgcga tgttaatcaa aggtggtaaa attcaaggtg gtttgacaga
     4861 agcccataac gatcaccgta ttgctatggc gctggcgatt gccgggctat gttcggaaaa
     4921 gccggttgtg atcgcgggcg ccgaatgtgt taccaagtcc tacccgacat tttttgatga
     4981 tttaaaatca gttggaggta atatcgatga atagttttgg acgactcttc cgcctaacga
     5041 ttttcggcga gtcacacgga ccggcggtcg gggtaataat tgacggttgc cctccgggat
     5101 tggaaattaa agaaagtgat ttaactgctg atctcgatcg ccgtcggagt ggtgctccag
     5161 gtacaacagc tcgtcaggaa agtgatcagc cacgattttt gagtggtgtt tggaataatc
     5221 acacaaccgg cgcgccgctg acgattgtct ttgagaacag cgcccagcga tcggcggatt
     5281 acgccgaaaa gccgaccgtc taccggccgg ggcacgctga ttttaccgct gatcgcaaat
     5341 ttcgaggctg gaatgatccg cgtggcgggg gacacttttc cggacggatg acggtcggct
     5401 tggtcgccgc cggcgtgctg gctaagaaaa ttattgcacc ggtcgaggtt tccgcttctc
     5461 tcatagaagt tggcggtcag gctgattatt cggaactgct cgagcaagcc cgccgcgacg
     5521 gcgactcgct gggcgggatc atcgaatgcc gcgctcaaaa tttgccggtc ggcctcggcg
     5581 aacccttttt tgattcggta gaatcggttc tggcgcatgc catctttgcc attcctggta
     5641 tcaagggtat tgaattcggt agtggatttg ctgccgctcg tatgcgcggt agtgaattca
     5701 atgactgctt catcgcaccg aacggcaaga ccgctacgaa tcattctggc ggaataaatg
     5761 gtggaattac caatggcaac gagctcgttt tccgcatcgt agctcgaccg actgccagca
     5821 ttgcccgcag tcagcaaact ttagattttg atagcggaaa aatgacagat ttatccatca
     5881 gcggtaggca cgatgtctgc tttgctttac gcctgccggt gatagttgag actctgacgg
     5941 cttgctgtct aaccgacttg ttattaattc aaaaatcatg gcaattaaat gaggaaaata
     6001 acttatgaac ctggatgaaa ttcggtcaca gattgacctg atcgacggca aaataatcaa
     6061 attgctcaat gaccgtctgt cacaggcaat tatggccaaa aaattcaaaa ccaatatcga
     6121 agaccctcag cgcgagcaag aggttttcaa taatctggaa ctaaaaactt acgacttggt
     6181 cactccggag ttcgctcgac gtttatacgc cattatcatc gaagaaggca aacgcttgca
     6241 atctcaagac ctaccggtaa tcgctttcca gggcgaacac ggcgcctata gcgaaatggc
     6301 tggcaaagcc tggaatccgc aattagtcag cattccttgc cgcgaatttg ccgaactatt
     6361 cgaaggcgtt agcgacggct cctacgatta cgggattatt cctattgaaa atactctcgg
     6421 cggtgtggtt gggccggtca atgaattgct catttccact aaattgaatg tcgtgggtgc
     6481 aatagaaatg ccgatacgtc attgtttact ggcggtgcgc aatagcgatt accgcgaact
     6541 gcgcagtgcc tattcccatc cgcaagcttt agcccagtgt aaacattttt tagaacgtaa
     6601 taaactcgac ccgataccct actacgacac ggcgggcgcg gctcgtatgg ttgctgaaaa
     6661 aggtttgaaa ggaattgccg caatcgccag tccgctagcc gctcaacttt acaatctgga
     6721 aattatcaaa gagcagatcg aagattcacc tttgaacact actcgctttt tgattctggc
     6781 tcatgaaccg ttcaccggaa aaggcgaaaa atgttcaatc attttctcga ccgctcataa
     6841 agccggtacg ctcttcaaag tgctcgaaat ttttgccaag gcgaaaatca acctgacccg
     6901 catcgaatca gtccccaaat cgcccggtca atatgccttc ttcctcgatt ttatcggctc
     6961 taaagatgat cccaaagtcg aaaaagtttt agaagaagtc gcacaatcta cctctgattt
     7021 ccgcttgatg ggatgctatt tagaaaggat tctggcgtga aaatatttat tctgggaacg
     7081 ggccgtatgg gtgcctggct ggtggaagaa ttttgtcatc aacaccaggt tgcagtttac
     7141 gatcgcgatt taaataaatt gaaatgtttc gacaatgttg aacgtttttc cgatttaaat
     7201 gaggtgacga gctttcaacc ggagctggct atcaatgccg ttagcccgca aaataccgta
     7261 tcggctttcg aggaactcct gccgctcatt cctgctaatt gtatgctgtc tgatattgcc
     7321 tcggttaaag ccggtgtggc tgaattttac cgtaaaatcg gccggcgctt cgtttcatct
     7381 catccgatgt tcggacctac ttttgccaat attcgcgatc tgcgccacga aaacgctatt
     7441 attatcagtg aatctgatcc ggcaggtaag gcttttttcc gtcatttttt cgagaacttg
     7501 caactgaata ttttcgaata caccttcgaa gaacacgatc agaccacggc ttattcttta
     7561 ggcacaccct ttgtttcgac gttgaccttc gccgccaatt tgaaaatgca gaaagtgccc
     7621 ggtactacct tccgcaaaca tcttgaaatc gcccgcggac tactttcgga ggacgattat
     7681 ctactatcgg aggtactctt taacccctac acgctccagc aaattgaact tatcaattct
     7741 cgtctgtcat ttctaaccca catcattcgg ggacgcgatt acgaggaaat gaaacgattt
     7801 ttagataact tgcggaaaaa catcagcgca ctaaaagaat aaccagcatt atttttctga
     7861 tactatttcc aatgctggcg ttaggttatg ccattcagct attcttatag tagttcggat
     7921 taatcccaaa attaggaacg atagaaattt ctatcattag ggtcaataat ttatttatcc
     7981 ggtttataca gtcctcccgg attcgagccc agcagaccgg cttttaacat gcgctgatca
     8041 actggtgcga gattttgttc catctcaggt gtgagtaaat ttacgcgacg ctgacgatcg
     8101 agatggttag cgatttcatc cagatgttca tcgtctatca tccaattcca gcggtcgcaa
     8161 atagtaagat tcagcggcaa ggtgtagccg cgcaaggttt tctcgccgac gatgttgaaa
     8221 taatcctcga aatatgaaag tattagctcc cgcagagtgc ggaaaatcgg ctcacggtag
     8281 cgcagaccga cgaagttgga tttagctact gcaccccagt gattactgcg tttgtaaacc
     8341 gccagcaaat gatcgtcgtc gcgctcgttg ggtaacattt ctacaatcaa tggtggatac
     8401 cccagtcgtc gcagggacgc cgctgcaaat agcgcaccat cgaagcaatg ggctcgacga
     8461 tcgtgcaaaa cactcagtgg tgatcggtaa ataggctcgg tgctataggg cgtttcatcg
     8521 aggaacgtct ggattttcag cggcgaatct aaactattca ttaaagcaaa ttccgtcgca
     8581 tttaaatgac gatcaaaatg ttttattggg tctagttgaa tcatttttcg atctttccaa
     8641 tttagtctgt ttatcaatga tgagttcgat tttgcaaaaa tatttatcct atgaatggga
     8701 ttctaaagga ataataggca ttctcaattt tcactatctt cttatcagca gtcctgaaaa
     8761 gtcgtagatg tagctcccta cgttcctggc actcaccaag ttaaattcgc cttttgttat
     8821 agattgaact cacccgatgc ttccggctgg tataaaactt tttcgattcg aattcgggta
     8881 attccagctg gaaccttcca ctcaatagta tctccctcag aataacctaa aagtgccgtc
     8941 ccgatcggcg aaaaaaccga aattttgcct ttattaatgt tagcatcaat aggaaagacg
     9001 atggtaactt ccattgtact gtcatcatcc agatcacgga aaattagacg cgtattcata
     9061 gtgacgattt tttctgggat ttcgctagct tcaataatct tggctctgtc aagctccttc
     9121 gccaatagtt gcaaatcgcc ccgtccatgg aaatatgaaa tcccagccat agaaagttcc
     9181 ttttttagac gttttaaatc ttgagaagta atataaatct ccctttcctt cgtcatattg
     9241 cacctttctc tattttttgc tttattacca atgcggttga ttgccgattt ccactccggt
     9301 gacatcatcg gcttccataa tggcaataaa tgcctgggca tctacttccg ttaccagacg
     9361 ttttaataat gaaactgaac gcgccggaat caccgaatat aacaatgtac acccagcccc
     9421 gcgatatcca ccctgtcctt taatcaaagt cactccaatt cgatgtgcgt ttaatttttc
     9481 gacgataatt tcccattgat cggagataat tatggcggtt ctacggtttg ccaagccgtg
     9541 aaaaatttta ttcgtaaaca gaccactaac gaaaatgaaa acaagagtgt aaaaaacttt
     9601 ctctattgga aaaataaatg ctgacaaaat caacaccacc gcattaataa gaatggcacc
     9661 ggtgcccagg gttattgaaa ataatttgtt caatattacg caaaggatgt ctgaaccccc
     9721 actggaacca taagaacgta aaattagcgc agtccccgca ccggataaag caccggcaat
     9781 aataacggct aaaagcggat cagatagatt aatttcgcaa ttaattaacg aaagagtcgc
     9841 agaataaatc accattccca ataacgagta tttgataaaa cggcgaccca caaaacgcca
     9901 gccgagcaaa aaaaccggga tattaataag acaataaagc aatcccagcg gaatcaccgg
     9961 ccatatatag taaatgatta aagcaaaacc cgtcattcca cgggcaagca gaccatgcgg
    10021 caggattaaa gctttcaccg ctagagcaca aagaatacta ccagacgtga gtaataaggt
    10081 ctgatataat aaattaacag ttccgttttt tgaaatgatt ctcatatcta ttacaattaa
    10141 actaatataa aagtgtatct tctttggcaa acataaacaa ttccagtgta taaaccaagc
    10201 tcatccggca gtgttcaaat cagcaaaact ttgaccgggt cgcattgggt attaatcaat
    10261 tatccatggc gatgagaaaa accttgtttt cggtctttat taagcttgaa attcgaccga
    10321 ttacctaagt ttttccaacc cgcctttggc aataaaaagt tcggcattga gaatgg
//
LOCUS       DAOMJU010000075        10361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6604_length_10361_cov_20.880943, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000075 DAOMJU010000000
VERSION     DAOMJU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10361
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6604_length_10361_cov_20.880943"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..179
                     /locus_tag="PLO58_07460"
     tRNA            104..179
                     /locus_tag="PLO58_07460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Lys,seq:ttt)
     gene            complement(652..1296)
                     /locus_tag="PLO58_07465"
     CDS             complement(652..1296)
                     /locus_tag="PLO58_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQO74664.1"
                     /translation="MAIKAVIFDFDGVVINSEPLYMVAERRLFDQYGIKVPDEDWKYF
                     KGTTEGDFYQLIMAKYQINTDVHKLIEDGRRYIKEEFNKGVDYLPGFLDFWQSIDGHY
                     RTGLVTSTSASFLEWVFRNTPVRNIFDLIITADDVRQAKPHPEPYLKAFESLRVRPEE
                     CVIIEDSMTGIQAAIASGAVTIGFRCDRNDRDMPKLHFYADNYTEIANILSKIG"
     gene            complement(1447..3066)
                     /locus_tag="PLO58_07470"
     CDS             complement(1447..3066)
                     /locus_tag="PLO58_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQO74665.1"
                     /translation="MISKKDLIIKIALRFIDVPRVEIDSRINESLRDLGEFYQTEYCL
                     VSALLPDTKEALKIYGLEDKSEDSVKLLQNIALANSPWLKNQLSLKGTVIINDVNNLP
                     AKANREKQELQQLEIKSLMIAPLVYNDTNFGLLIVAVSKTSRRWLDSEIELLKIVASI
                     ITSALHRKKTEEKMAETMSLYHLFIDKINEGIAICHKERFIFVNNKLAEMLGYTVDEL
                     IGMEFHNILTNRGVEILKERERRQAIGKTVPKRYETSFKCRDGHTIEVEVSPVEIEYR
                     GKLTTFAVISDISEYKRLSLAKQALEVKWLKQQRLMSLTLMIGGIIHNIKNTLTVVMG
                     RAQMLKTRLPELKEPDIIVDNVRKIESLLTSFIDKMNLELENKKITINLSELLRNELL
                     FLETESFFKTQIEKEIVLRRDLPLITGHYSDFSQSLMNIIHCCVESMRDSPRKILKIE
                     TGISQDNIYVEIATTGQPIIPDNAEELFTPSFYHEIPENENASDQKALIKYNLYNAYM
                     LLSSYKAKFSAFNNPESGSTIRVELPIESNN"
     gene            complement(3169..3789)
                     /locus_tag="PLO58_07475"
     CDS             complement(3169..3789)
                     /locus_tag="PLO58_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQO74666.1"
                     /translation="MSRTNNLIKAVFFDLGNVLVKVNLESAIPQIAELFRIPEKAVMQ
                     APILALEKKFERGQLTIAEYLVEVKNIYHIDHSIGVDDLERIWQTPFELNTDVWQIAL
                     RLRRQVPLFLLSNTNALHIRAIRRKFNILENMDGLILSFEVGATKPESAIYQYALRKA
                     DVTAEQALFIDDLKENVAGAERLGLKAHQFISASDLENILSQYSFK"
     gene            complement(3824..4807)
                     /locus_tag="PLO58_07480"
     CDS             complement(3824..4807)
                     /locus_tag="PLO58_07480"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HQO74667.1"
                     /translation="MLLVSKLVRGNAVDSFHIGYAVVVDENGEILFSAGDPEYPIFIG
                     AAANPFQAVAMLESGALEQFNFNDEELALMCGTHTGEHYHLDVLNSILKKMDLNIDNL
                     LCGVHPPLDRQTYEQLAIQGRRPTALHNANSGAHAGMLAIAKALKYDLSNYTEINHPA
                     QQKIFEKIKLYSGKERIPVATDNSNAPTYLMPIYNLAIMYKKLVEGSDDYLRRVFHSI
                     SLHPKLIGGRAQFDTDFGALMGGRGISKFGMEGVRGIGLRNNENNPIGIAIKVLSGNR
                     EACDSMAVAVLKHKHLIDDEIAKKLDSYLSSAVKSYTDVEIGKFFTEVVAE"
     gene            complement(4823..5521)
                     /locus_tag="PLO58_07485"
     CDS             complement(4823..5521)
                     /locus_tag="PLO58_07485"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQO74668.1"
                     /translation="MHYLEINLDPFIELGRIVDIPLGNPLNLLGFIEAVGVAGISFTN
                     TPERKGMINITALRSMSRCRLNIRANIENQAIQRAMSAKPDSITLINLDESDLTVDPH
                     SQIVHNLIETVQSAEDFNLILRLKPEIRDLKAAYRLKVDEVELATDPLVTQVLQVSYT
                     DELNKIVAASRLAIRNHMRVSLGGRLQKRLILTLLELIDVEFISVGRALLGQALITGF
                     DSALRELLEIVENR"
     gene            complement(5526..6686)
                     /locus_tag="PLO58_07490"
     CDS             complement(5526..6686)
                     /locus_tag="PLO58_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HQO74669.1"
                     /translation="MAQTSNYLKKPSKIVEKRHRDEKRLNALFSTVIGMQIILLVYFL
                     PKMAPSRANLFSEGKSRLINVARPFLLERGCQEIKSSSVATDRVSLIFELPASRDCEL
                     LAQQVSAYLNEHGFMVKKIDNLVKGTGFTLFVDFQHIPIGTLAFIKTDRPYPARMPIS
                     GVKPKLAIIIDDFGYTNQEIVSNFLKLPTKLTISVIPGHPYSQWVARNAKLAGKEVII
                     HMPMEPEQSSNSHGEDKYMLLTGMTPEEISHRVEMACAELPEAVGMNNHMGSLFTSDP
                     ELMQIVINSLKRKGLYFIDSMTSAESVAYEVAIRNQIPTALRTVFLDNVRDKGEIQAQ
                     FERAIEIASRGGHAVAIGHICSETLSALTEILESGLLNEVELTFASEVTSLK"
     gene            complement(6795..8150)
                     /locus_tag="PLO58_07495"
     CDS             complement(6795..8150)
                     /locus_tag="PLO58_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO74670.1"
                     /translation="MDKIGTDVELKPPSARYRWLVFFFISMAMFGNYYIYDSISPLAD
                     LLKAQLGYSDANIGLLNAIYSFPNIIMVLIGGLIIDRIGTRKSVLIFTILVMLGAVIT
                     ALSGNLLLMATGRLIFGLGAESMIVAITTILARWFKGKELSFAFGLNLTFARLGSFLA
                     LNSPTWGRSLYTSWQKPLLVTAVAGMMALFFIGIYCLLDLLASRRLLLPQESKQDKIE
                     LREVFRFGAGFWYITALCVTFYSAMFPFQTFAIKYFQEAHGTSREVGGNLSSMLTLAA
                     MIFTPLFGLVADRTRRKAWLMALGSLLIIPVYLIMAYHIDLATPLGFHDIINIKFSFF
                     GINSSIPAYLIIPMAMMGIAFSLVPAVMWPMVAIIVDNNRLGTAYGLMTMIQNIGLFS
                     FNIIIGLANDFTGASADNPGGYNLGMWIFSILGFCGLLFAFRLGKYEKSMHHEQIKEN
                     G"
     gene            8321..8488
                     /locus_tag="PLO58_07500"
     CDS             8321..8488
                     /locus_tag="PLO58_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQO74671.1"
                     /translation="MAYVISEDCTACGTCIDECPAEAISEGDIYKIDPDLCTDCGACA
                     EVCPSEAIHPA"
     gene            8816..9502
                     /locus_tag="PLO58_07505"
     CDS             8816..9502
                     /locus_tag="PLO58_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74672.1"
                     /translation="MSSRRWRAGAGLTEIQRHIHWVADLPDIITVILSDGSLNELETL
                     FDNHKIILAPNNGTEIYAADFSWNLPNLNLIRQELTDIYCAVRESLGAKLQPEYLGFS
                     QSELWLKVSPGAEAVRKSLIEQFKLQNPAHLRLITQSNQTTIVPLIKWDRGQVIQKLM
                     DLIPHPLNQTPLVIYFGVEENDEAAFQYVNQVGFSVIIHNRIPRATEACYYLRNVAEL
                     SKFLFWVHSR"
     gene            complement(9567..9818)
                     /gene="rpmA"
                     /locus_tag="PLO58_07510"
     CDS             complement(9567..9818)
                     /gene="rpmA"
                     /locus_tag="PLO58_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQO74673.1"
                     /translation="MAHKKGVGSSRNGRDSRGQRLGVKRFGGEQVSAGTIIIRQHGTK
                     IHPGENVGIGSDDTLFALIDGKVKFSRKGRDKKIVSVLA"
     gene            complement(9824..10237)
                     /gene="rplU"
                     /locus_tag="PLO58_07515"
     CDS             complement(9824..10237)
                     /gene="rplU"
                     /locus_tag="PLO58_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQO74674.1"
                     /translation="MNNQNYAIAEIAGDQVILEPGKNVAVPKMDLEVGAKFLANKILF
                     IHTGNDIKVGKPYLEDFAIETTVLEHKRNSKVVIFKKKRRKGYRVKKGHRQPYTVLQV
                     SEFAKPEAAAPVKKSRAKTKTVSESAEPAEQEKKE"
BASE COUNT         2971 a         2294 c         2001 g         3095 t
ORIGIN      
        1 acaataaaaa tttatacgtc atatccctaa aatggtcgat cgatagacag atttttcctt
       61 tgataatcgt tggtatattg gtaattttgc acgctttttg tcagggtgca tagctcagtc
      121 ggtagagcaa cggcctttta agccgtgggc cgcaggttcg atccctgctg cactcaccaa
      181 tccaaattta tcattgatat ctaaatttac gagcaagtcc cctgtttgcc ttattttcca
      241 agtggtaccc aaatccggca ccgagatgca agcctcataa caggttgtca ataattcgaa
      301 ccattatggg catcttttcc tgacagttca attcaacttt gggtggttgt gttaaaatag
      361 taaaaccaat ataatccgaa agggaaaggc tttgacgtta ctgactttag catactcagg
      421 tgaaccgttt aaagctttta ccacttaaaa tcaaatagaa ttggctctga attcaaggac
      481 gaacaatttt gcaggtcggt ctaaattcca ggttttagcg accgataacc ctgttcggtt
      541 ctcactccat cagattgttt aattcagacc atcactaaat tatataagat gagagccggg
      601 taaacgtctt cgccctgtct tgtatatttt taatttccta ccgaaataca gttatccgat
      661 tttagagagt atgttagcga tttctgtata attgtcagca taaaaatgta atttcggcat
      721 atcacgatcg ttccggtcgc accgaaaacc gatagttacc gctcccgaag cgattgcggc
      781 ttgaatacca gtcattgaat cctcaattat tacgcactct tccggtcgaa ctctcagact
      841 ttcgaacgct ttcagatatg gttcgggatg aggcttagcc tggcgtacat catcggccgt
      901 aataattaaa tcaaagatat tgcgcaccgg tgtgttacga aatacccact ccaaaaaact
      961 ggctgaagtc gaagtcacca gcccagtgcg ataatggccg tcaatgcttt gccagaagtc
     1021 gaggaaaccg ggcaaataat caaccccctt gttgaattct tccttgatat agcgccgccc
     1081 gtcttcgatt aatttatgaa catcagtatt aatctgatac ttagccatta tcaattgata
     1141 aaaatcaccc tcggttgtcc ccttaaaata tttccaatct tcgtccggaa cttttatccc
     1201 atactggtca aataagcggc gttctgcgac catatataaa ggttcggagt taataacgac
     1261 cccatcaaaa tcaaatatta cggccttaat tgccatcctg ttttaatcct aatcgtaaat
     1321 tactatatta actatcattt tatattctgt actgttaaaa tcacaagtaa tacgccaaaa
     1381 cataaattat tatttctaac caattctcct gattgtccca taattacaag ttactattgc
     1441 taaaaattaa ttattgctct cgatcggaag ttccactcgt atagttgagc cgctctccgg
     1501 attattgaaa gcagaaaatt tggctttata gctactcaat agcatataag cattgtataa
     1561 attatactta atgagggctt tctggtcgga agcgttttcg ttttcaggaa tctcatgata
     1621 aaaactaggg gtaaaaagtt cctcggcatt atccggaata ataggctgtc ccgtggtagc
     1681 aatttctacg taaatattat cctgggatat accagtttcg attttcaata tttttcgagg
     1741 agaatcgcgc attgattcaa cacagcaatg gatgatattc atcaaactct gcgaaaagtc
     1801 actataatgg ccagtaatca gcggcagatc gcgtcttaag acgatttcct tttcaatctg
     1861 ggttttaaag aaagattcgg tctcaagaaa taacagttca tttctaagca attcacttaa
     1921 attgatcgtg attttcttgt tctccagttc gaggttcatt ttatctatga aactggtcaa
     1981 tagagattct atttttcgta cgttgtccac aatgatgtcc ggctctttta actccggcag
     2041 acgggttttg agcatctggg cccgtcccat taccaccgtg agcgtatttt taatattatg
     2101 gataatgcca ccgatcatta aggtcagcga cattaaccgt tgttgcttaa gccattttac
     2161 ttccagagct tgtttagcca ggctaaggcg tttatattcg gaaatgtcac tgattacggc
     2221 gaaagtagtt aatttacccc gatactcgat ttccaccgga ctgacttcca cttcaatcgt
     2281 atgaccatcc ctacatttaa aggaagtttc gtatcgcttg ggaacggttt taccgattgc
     2341 ctgccgacgt tcgcgctcct tgagtatttc gactccgcgg ttcgtcaaaa tattatgaaa
     2401 ctccataccg attagctcgt caaccgtata accaagcatt tcggctagtt tattattaac
     2461 aaatataaag cgctctttat ggcagatagc aataccttca ttgattttgt caataaataa
     2521 atgatagagg gacatggttt cagccatctt ctcttcagtt ttctttcggt gaagggcaga
     2581 ggtaatgata ctggcaacaa ttttcagcaa ttctatttca ctatccagcc accgccggga
     2641 agtttttgat accgcgacta ttagtaatcc gaaattagta tcgttatata ccagcggtgc
     2701 aatcattaaa gatttgattt ctaattgctg taattcctgc ttttctcggt tggcttttgc
     2761 cggtagatta ttaacgtcat taattatgac agtacctttt aaactcaact gatttttcaa
     2821 ccagggggaa tttgctaatg caatattttg caaaagttta acggaatcct ctgatttatc
     2881 ttcgagccca tatattttca gcgcctcttt tgtgtccggc agtaatgccg ataccaaaca
     2941 atattcggtc tgataaaatt cgcccagatc ccgtagggat tcatttattc tgctgtctat
     3001 ttcaactctg ggtacgtcaa taaatcggag tgcgatttta attattaaat ctttcttact
     3061 aatcatattg tgctcacttt tcattctgca gggaaggtat tcgattttat taaaatccgc
     3121 aaataggata aatgttgaac agatttgctt ctgatactat tgatgttcct atttaaaact
     3181 atactggctt agaatatttt caagatcact cgcagaaata aattggtggg ctttaaggcc
     3241 gagtcgctcg gcgccagcga cattttcctt aagatcatca ataaataggg cttgttccgc
     3301 ggttacatcg gcttttcgca aagcatattg ataaatcgcc gattccggtt ttgtcgcccc
     3361 gacctcaaat gataaaatca atccatccat attctccaaa atattgaatt tccggcggat
     3421 cgcccgaata tggagggcat tggtattgga taaaagaaag agcggtactt gccgacggag
     3481 tcttaaagca atctgccaga cgtccgtatt caactcgaag ggagtctgcc aaatccgttc
     3541 gaggtcatca acaccgatgc tgtgatctat gtggtaaata tttttgactt ctactaaata
     3601 ttctgcaatg gttaattgac cacgctcaaa tttcttctca agtgcgagaa tcggcgcttg
     3661 catcaccgct ttttccggaa tacggaatag ttccgcaatt tggggaatag cagattcgag
     3721 attgactttg accagaacat taccgagatc aaaaaaaacc gctttaatta ggttgtttgt
     3781 gcgtgacatt ggttatataa ttatcgtctt aggtaatttc cggctattcg gcaactactt
     3841 ccgtgaagaa tttgccgatt tcgacgtcgg tataactttt tactgccgaa ctgaggtagg
     3901 aatccagttt tttggcaatt tcatcatcta tcaagtgctt atgctttagg actgcgaccg
     3961 ccatcgaatc acaagcttcc cggttaccac tcagcacctt tatagcaata ccgatcggat
     4021 tattttcatt attccgcaga ccaattccgc gcacaccttc cataccgaac ttagaaatac
     4081 cgcgccctcc cattaaagcc ccaaaatcgg tatcaaattg ggcgcggcct ccgattagtt
     4141 tcgggtggag ggaaatgcta tggaaaaccc gtcgtaagta atcgtcgctc ccctcgacta
     4201 attttttata cattattgcc agattataaa ttggcattag ataagtcggg gcattactgt
     4261 tatcagttgc gaccggaata cgctctttgc cggagtatag ttttattttt tcaaagattt
     4321 tttgctgggc gggatggttg atctcggtat aattggacaa atcgtacttt aaagccttgg
     4381 ctatcgcgag cattccggcg tgagcccctg aattggcgtt gtgaagcgca gtaggtcttc
     4441 gtccttgaat agctaattgt tcataagttt ggcgatcgag gggcggatgc actccacata
     4501 gtaaattatc tatattgagg tccatttttt tcaggatgga attgagcacg tcaagatgat
     4561 aatgctcacc ggtgtgagtt ccgcacatta aagccagttc ctcatcgtta aaattaaatt
     4621 gctccagcgc tcccgattcc aacatagcga ctgcttgaaa aggattagca gcggctccaa
     4681 tgaaaattgg atattcagga tcccctgccg aaaataggat ctcgccattt tcatctacca
     4741 caactgcata accaatatga aagctgtcaa cagcatttcc tcttactaac ttacttacca
     4801 gtaacatacg aaaacatctc ctttatctat tttcaacaat ttcaagtaat tctcttaaag
     4861 ctgagtcaaa tccggtgatc aatgcctgac ccagtaatgc ccgacccaca gaaataaact
     4921 ccacatctat caattccaaa agagttaaaa tcagacgctt ctgcaatcgt cccccgagtg
     4981 aaactctcat atgattgcga attgccaaac gcgaagccgc aacgatttta ttcagctcgt
     5041 cggtgtagga tacctgtaat acctgggtga ctaatggatc agtagctagt tccacttcgt
     5101 caactttcaa acgataagcc gcttttaagt cgcgtatctc cggtttcaac cgtaagatta
     5161 aattgaaatc ttcggcactc tgcaccgttt ctatgagatt gtgtactatt tgagaatgag
     5221 ggtcaaccgt caaatccgat tcgtctaaat taataagggt gatggaatct ggtttggcag
     5281 acatagctcg ctgaatagct tgattctcaa tatttgcccg gatattcagc cgacagcggc
     5341 tcatagaacg cagtgctgta atgttaatca ttccttttcg ttcaggtgta ttcgtgaaac
     5401 tgattccggc cacaccgacg gcttcgatga aacccaataa atttagcgga ttacccagcg
     5461 gaatatccac tatccgacca agttctataa aggggtcaag atttatttct aagtaatgca
     5521 tatttttatt tcaaagaagt tacttccgaa gcaaatgtaa gttcaacttc gtttaaaagg
     5581 ccggattcga gaatctcggt cagggctgac aaagtttcgg aacatatatg gccgatcgcc
     5641 acagcgtgcc ctccgcgact ggcaatctca atcgcccttt caaattgcgc ctgaatttcg
     5701 cctttatccc taacattatc aagaaatacg gtccggagtg ccgtcgggat ttgattgcgg
     5761 atggcaactt cataagcgac actttcggcc gaagtcatac tatcaataaa atacaatccc
     5821 ttgcgtttca gactattaat cacgatttgc atcagttctg gatcactcgt aaatagcgag
     5881 cccatatgat tattcatacc cacagcttcc ggtaattcgg cgcacgccat ttcaactcgg
     5941 tgactgattt cttccggggt cattccggtt aagagcatat acttatcttc gccgtgacta
     6001 ttagaacttt gctctggctc cattggcatg tgaataatca cttctttacc cgctagcttg
     6061 gcgtttcgag ccacccattg tgaataaggg tgaccgggaa tgacggaaat cgtcaattta
     6121 gtcggcaact ttaaaaaatt acttactatt tcttggttag tatagccaaa atcatcaata
     6181 ataatcgcca atttgggttt tactcccgag ataggcattc gggctggata aggtctatcc
     6241 gtcttgatga acgccagagt tccgatcgga atatgttgaa aatcaacgaa aagagtaaat
     6301 ccggttccct taacgaggtt atctattttt tttaccataa aaccgtgttc attaagataa
     6361 gcactaactt gttgagcaag tagttcacaa tccctactgg ctggtaattc aaatatgaga
     6421 gacactctat cggtggcaac tgaggaactt ttaatttcct gacaacctcg ttctagcaaa
     6481 aaggggcgag caacatttat tagcctactc ttaccttcag aaaaaaggtt agcccgagaa
     6541 ggagccattt tcggtaaaaa atagacaagc aaaattatct gcataccaat gacggtagaa
     6601 aacagtgcat tcagtctctt ttcgtctcgg tgacgttttt cgactatttt agatggtttt
     6661 tttaaataat tgctcgtttg ggccatagta ttttaatcac gtaaaaatac taaattccga
     6721 ctgattctca aaattgtgct acaaaaaatt atccacttcc tttagtggca aactacctaa
     6781 cggttcaagg ctgttcaacc gttctccttt atttgctcat gatgcatcga cttttcatat
     6841 ttacccaacc gaaaagcaaa tagcaatccg caaaatccaa ggatcgaaaa aatccacatt
     6901 ccgagattat aaccacccgg attatctgcg ctggcaccgg taaaatcatt tgccagaccg
     6961 attataatat taaaactgaa cagtccgata ttttgaatca ttgtcattag accataggca
     7021 gtgcccagtc ggttattgtc aactatgatg gcgaccattg gccacattac tgctggtacc
     7081 agcgaaaagg caattcccat catcgccatg ggaattatca aataagctgg aatacttgaa
     7141 tttataccga agaaactaaa ttttatattt attatatcat gaaaacccag aggagtcgcc
     7201 agatcaatgt ggtaagccat tatcagatac accggaatga tcaacagtga gcccaacgcc
     7261 atcaaccagg cttttcgacg agtgcgatct gccaccaatc caaataacgg cgtaaaaatc
     7321 atcgcggcta aagtcaacat actggataaa ttaccgccaa cttcgcgaga cgtaccatgc
     7381 gcctcctgaa aatatttaat agcaaaagtc tgaaaaggaa acatcgcaga gtaaaatgtt
     7441 acgcacaagg cagttatgta ccagaaaccg gctccaaatc gaaaaacctc gcgtaattca
     7501 atcttgtcct gcttactttc ttgaggcaag agcaaccggc gagatgccag taaatccagc
     7561 aaacaataaa taccgataaa aaacaatgcc atcatcccag ccacagccgt taccaggagc
     7621 ggtttttgcc agctggtata aagactacga ccccaagtcg gcgaattgag cgccagaaat
     7681 gaacccaacc gggcaaaggt tagattcaat ccgaaggcaa aggataactc cttgcctttg
     7741 aaccagcggg ctaaaatcgt tgtaatagcc acaatcattg attccgcacc taacccgaaa
     7801 atcagtcgtc cggtcgccat taataataga ttgccgctca gggcagtaat aactgcgccc
     7861 aacataacca aaatcgtgaa aattaggacc gatttacgag ttccgatccg gtcaataatc
     7921 agtccgccga ttaaaaccat gataatgtta ggaaagctat aaatagcatt taatagcccg
     7981 atattggcat cactatagcc cagctgagcc ttcagaagat cagctaatgg gctaatactg
     8041 tcatagatat aataatttcc aaacatcgcc atactgataa aaaagaaaac taaccagcga
     8101 tatcgggcgc taggcggctt cagttcgaca tccgttccta ttttgtccat ttccagtcct
     8161 tacaaaaatt cacacaccat cataacaatt tagcggcagt taatctaagc ataccctatt
     8221 tcaatacaaa gaaatctgac gggatattgt ctggattgct ggctttattt aaatttcggg
     8281 cgatttttga ttcacgaaat aattacgagg agttatagta atggcttatg taattagcga
     8341 agactgcacc gcttgcggga cttgcattga cgaatgccca gctgaagcaa ttagcgaggg
     8401 tgatatctat aaaatcgatc ccgatctatg cactgactgt ggggcttgtg ccgaggtttg
     8461 cccctccgaa gctatacatc cggcataatt aaatttgccc tcaaggcttt agccggatta
     8521 aatatttttg aattagagtg aattcacgtt tttgtttatt aattttgggc agaatttggg
     8581 cttagggttt ttgcagacaa ataactgatt cagaccactt acaaagtttt tccgattggc
     8641 tttaaaccac agaatgctat ttaaaataag actgattata ataagcctta taaatccttt
     8701 atcaattcaa taaatgcaat cagcacctat ttcacttttt gacaaaattc ccgagataag
     8761 ccagtccctt gcaaatgggc gacctcttgg tttagtattt aatctcgaat gccttatgag
     8821 ttcacgccgt tggcgggcgg gcgcgggtct caccgagatc cagcgtcaca ttcattgggt
     8881 tgccgactta ccggatataa taaccgtcat tctaagcgat ggttcgctta atgagctcga
     8941 aactctattc gacaaccaca agataatttt agcacccaat aacggcactg aaatttatgc
     9001 ggctgatttt tcctggaatt tacctaatct taacttgatc cggcaggaat tgaccgatat
     9061 ttattgtgcg gttcgtgaat cccttggcgc taagcttcag ccggaatatt taggtttttc
     9121 tcaaagcgaa ctctggctca aggtttcgcc gggggcggag gcagtccgaa aatcattgat
     9181 tgaacagttc aaactgcaga acccggcgca tctccgtctc ataacccaaa gtaatcaaac
     9241 gactatcgtt cccctcataa aatgggatcg cggtcaagtc attcaaaagc tgatggattt
     9301 gatcccccac ccccttaatc aaactccttt agtaatttat ttcggagtag aggagaatga
     9361 cgaagcggct tttcaatatg taaatcaagt cggcttttca gtgatcattc ataaccgcat
     9421 tccacgtgct acggaagctt gctattatct gcgtaatgtc gctgagctaa gtaaattttt
     9481 gttctgggtt cactcccgct aaggttttcc cagaaaataa aaatcctcca cgcaggagga
     9541 ttaatgagca atttttgatt tgataatcag gctaaaacgc tgactatttt cttatcccgt
     9601 cctttacgtg agaatttcac tttgccgtct attagagcaa aaagggtgtc atcactgccg
     9661 attcctacat tctcgcccgg atgaattttc gtaccatgtt gacggataat aattgtacca
     9721 gccgatacct gttcgccacc aaaacgcttg acacccagcc tttgaccgcg actgtcgcgt
     9781 ccattgcgtg aacttccgac gcctttttta tgagccatac ttactactcc tttttctctt
     9841 gttcggcagg ttcagccgat tcgcttacag ttttagtttt cgcacgactt tttttaaccg
     9901 gcgcggcggc ttccggtttg gcaaattcgc ttacttgcag aacggtatat ggttgacgat
     9961 gccccttttt tacgcggtat cccttgcgtc tttttttctt aaaaattaca accttactgt
    10021 tacgcttatg ctcaagaaca gtggtttcaa tagcaaaatc ctccagatag ggtttgccaa
    10081 ctttaatatc attgccggta tgtataaata agatcttatt cgccagaaat ttagcgccaa
    10141 cttcaagatc catcttggga actgcgacgt ttttccctgg ttctaaaatc acctgatcac
    10201 cggcgatttc agcaattgca taattttggt tattcataat tctctcgtga ttactatcaa
    10261 aaaaggctgg gaaatttaga tataaagact gaataatgca agatgccggt cggatatttt
    10321 tgtctgatag ggaagactaa gacaggagtt gaggctaagg g
//
LOCUS       DAOMJU010000076        10268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6687_length_10268_cov_14.064036, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000076 DAOMJU010000000
VERSION     DAOMJU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6687_length_10268_cov_14.064036"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1927)
                     /gene="ftsH"
                     /locus_tag="PLO58_07520"
     CDS             complement(47..1927)
                     /gene="ftsH"
                     /locus_tag="PLO58_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQO74675.1"
                     /translation="MKKNEKFSLIYFWLITLMIIFTQLLFFPDRPANVRLSYRDFLNA
                     LDSGKVARIVIFEDRIVGEFKSEDINEEQALTHAPTAPWRLRVPSIEKQVARQFIVSR
                     IPELPDPELLAKLQASGVNFEGRYESNSLRNFFLNWVFPIIFFIGLWGVIFRRMGSSN
                     PMLDFGRNKAKIYAEKPENQVKFSDVAGVDEAVEEVKEVVDFLKNPKKYTRLGGKLPK
                     GLLLVGPPGTGKTLLARAVAGEAGVPFFNLSGSDFVEMFVGLGAARVRDLFKQAKAAA
                     PCIIFIDEIDAIGKSRGNALTYGGGYDERENTLNQLLVEMDGFDPGVGVIIIGATNRP
                     EVLDPALLRPGRFDRQVLVDRPDMKGREAIFKVHTRNLKLSSNVDLKQLAAQTPGFAG
                     AEIANVCNEAALLAARNNRDEVTQSDFEAAIERLIAGLEKKNKIINEKERKIVAYHES
                     GHAIVGYFTPGAEQVQKVSIVPRGIGALGYTLQMPLEDRYLMTKSEIIGKIKGLLGGR
                     AAEEIVFNEISTGASNDLERVAQLVKNMIVVYGMSEKLPNYSLVNRSTPGFLNQSAGI
                     ERRSEHLERLIDEEVKEIIDKCYRETKEMLIEKRELLDKMANTLLEKEVLEKEEIEAI
                     LS"
     assembly_gap    2259..2358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2716..3057
                     /gene="yidD"
                     /locus_tag="PLO58_07525"
     CDS             2716..3057
                     /gene="yidD"
                     /locus_tag="PLO58_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HQO74676.1"
                     /translation="MLSQEHQKWTDYWEIPLNLPEKPGEIFSTNLFSSSAIAWIKIYQ
                     KVIASQDLPSCVFHPSCSRFALTSIQEFGVVKGALLASDRLMRCNPFAMRYYPFDGEK
                     LADPPQKYRLK"
     gene            3070..3906
                     /locus_tag="PLO58_07530"
     CDS             3070..3906
                     /locus_tag="PLO58_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74677.1"
                     /translation="MKKSILFLLILSISTTANAQIESSLFEPAHLKEFGLYLFQQKDY
                     LRAAMELERYRYFAKSGDDSITFVIGLCHQFRQRYDFAAATFRELAEDDSSKFKESAR
                     LAMLYNYTKLEDWPKILGLSYQNDAEFYFYYLASVKTAPTTITTSFFQNVNDESLRNQ
                     LKALEQDRNKIHPKSPFLAGTLSTLIPGLGKVYINRSGDALFSFGMINLFALLTYKAF
                     QAELPVTGVLSGGLTLSLYLGTIYGSYVGAELYNRNLDADWQSKMTELNPVDRHPYWS
                     TW"
     gene            3900..4733
                     /locus_tag="PLO58_07535"
     CDS             3900..4733
                     /locus_tag="PLO58_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74678.1"
                     /translation="MVKRIILLLLAIFSVIFSTENLGDYYNAKQHYFEAITEYKRQLF
                     FGQYESQDEILLKMAKVYHAVQQDLFAEEILLQAISNDEASAFDWECSLELARLHWDN
                     YNYFEMRNVLDFISINSKQPIQDTINYIKAWTYFYEAKWGEGLALLDSVDFIDSKSLQ
                     KEISGVHKIPQKSRTLALVMSNIIPGTGHLYAHDYQNALYSSLLVGGTMVSIISNVID
                     RAYFVALIKYLFIYSRYNNGTLVNLAKKVDRDNIDAIGNYLKTVSQKYPAPLELLECL
                     R"
     gene            5341..8487
                     /locus_tag="PLO58_07540"
     CDS             5341..8487
                     /locus_tag="PLO58_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQO74679.1"
                     /translation="MKRYFVSLVVFTLAGFLAYAQELPANRSSLNSPLHNSGYSEEIL
                     GKSEQFNRHPYSKFLMDLDEQPYRHPCPSESRDATSTLNLNNTATTNKEPEWISYYAS
                     GLLPSPDIATSIAVDKHGNVYVTGYSVEALFGADYCTIKYDNSGNPIWAVRYDGGGDD
                     CAYALVIDGSCNVYITGYSEGSETGLDYTTVKYNSNGEEQWVARYIGPENLDERAFAL
                     VIDDSGNVYVTGYSYSPRTRSDYTTVKYNSNGEEQWVAIYNGPVNNEDCAKAIAIDNS
                     GNVYVTGGSYGSGTVSDYATVKYNSNGEEQWVVRYDGPGNDWDEANALAIDSSGNVYI
                     TGFSYGSETENDYATVKYDQNGEEQWVTRYNGSENSHDKGNALVIDGAGNVYVTGYSN
                     GSITGADYATVKYNSNGEEQWVTRYNGPGNSDDESYALAIDGSGNVYVTGYSYGSGTV
                     TDYATVKYNSNGEEQWVARYNGPESDYYDYDYAYAITVDDSGNVYATGYSHSLGTVED
                     YATVKYNSNGEEQWVARYNGPGNSEDKARDIAIDGSGNVYVSGSSYCSQTGYDYATVK
                     YNSNGEEQWTVRYNGPGNSDDESYALAIDGSGNVYVTGYSYGSGTATDYATVKYNSNG
                     EEQWVARYNGPGNSADEAYSMVIDGYGNVYITGFSYGSTTEAEADYATVKYNSNGEEV
                     WVSRYNGPRNFDDEAYSIVIDGSGNVYVTGFSYGSITGADYATVKYNSDGQEQWVARY
                     DGPGNFDDEAYSIVIDGSGNVYVTGFSYGSITGADYATVKYDSNGKEQWVVRYDGIDN
                     SSDYGKAIAIDGSGNVIVTGYSDSLGTRSDYTTIKYNSSGEEQWIARYNGPGNDYDKA
                     YNLAIDDYGNVYVTGESRDLKKGYDYATVKYDSNGEEQWVARYNGPGNDDDKAFAFAI
                     NSSGNVYVTGISDGAGWSVYTTIKYSSDGNRIDEEKLKTPVYSLSQNYPNPFNSATTI
                     TYSISSQSLVKIEVYNIIGQKVLTLEDGIKTPGVYTARFSPRADFTSGIYFYRLDVLD
                     MSNKSKRFSEIRKMVLIK"
     assembly_gap    8619..8718
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8887..9621)
                     /locus_tag="PLO58_07545"
     CDS             complement(8887..9621)
                     /locus_tag="PLO58_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HQO74680.1"
                     /translation="MKEFKIGCCGFPIEKEQYYREFNCIEINITFYQLPQIVTAEKWR
                     LAAEKQNPKFEFVIKAWQIITHPAGSFTYRRLREKIGAKENYGLFKDTDEVFTAWERT
                     REFALKLGCTKILFQCPPSFTPDEKYLLNLYNFFEKIRKDSEDFEFILEVRNPKWDKK
                     TIQKVCRELNLIHCVDPLYQPSYYGDYRYYRLHGLHIGNRLDYNYTFSDQELEKVLAL
                     CDQELNYVMFNNFTMYSDALRLMRYT"
     gene            complement(9829..10146)
                     /gene="trxA"
                     /locus_tag="PLO58_07550"
                     /pseudo
     CDS             complement(9829..10146)
                     /gene="trxA"
                     /locus_tag="PLO58_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
BASE COUNT         3008 a         1900 c         2048 g         3112 t
ORIGIN      
        1 cttaagttct ttcttaatct ctatgattat ctattatccg aattaatcag gataaaatcg
       61 cctcaatttc ctctttttct aacacttctt tttctaaaag cgtatttgcc attttatcca
      121 gtaattccct tttctcaatt aacatctctt tggtttcacg gtagcattta tcaataattt
      181 ctttgacttc ttcgtcaatc aatctttcga gatgttctga gcgcctttca attccggctg
      241 attggttgag aaatcccggt gttgaacggt tgactagaga ataattcggc agtttttcgc
      301 tcataccata cacaacgatc atatttttca ctaattgagc cacgcgttcc aaatcattgg
      361 aagcgccagt cgagatctca ttaaagacga tttcttcagc ggcacgcccg ccgagtaatc
      421 cctttatttt tccaataatt tcagatttag tcatcaggta acgatcttcg agtggcattt
      481 gcaaagtata accgagcgca cctattcctc tcggcactat ggatactttt tgcacctgtt
      541 cggcgcctgg agtaaaatat cctacgatcg catgacccga ttcatgatag gcaacgattt
      601 tacgttcttt ttcgttgata attttatttt tcttttccaa accggcaata agacgttcga
      661 tagcggcttc aaaatcagat tgggtaactt catcgcgatt gtttcgggcg gctaaaagag
      721 cggcctcgtt acatacattg gcaatttcag caccggcaaa tccaggcgtt tgggcggcta
      781 attgctttaa atcaacattc gagctgagtt ttagattacg agtatgcact ttaaatatcg
      841 cttctcgacc tttcatatcg ggacggtcaa ccagtacctg ccgatcgaat cgtccgggtc
      901 ttaggagagc ggggtcaagc acttccggtc tgttagtagc tccgataatt attaccccta
      961 ctccgggatc gaaaccgtcc atttcaacca gtaactggtt gagcgtattt tcacgttcgt
     1021 cgtagccgcc tccgtaagtc agagcattac cacggctttt cccgatagca tctatttcat
     1081 caatgaaaat aatgcaagga gcggcggctt tggcttgctt gaacaaatct ctgacccgag
     1141 ccgcgcctaa gccgacaaac atttccacaa aatctgaacc gctgaggtta aaaaatggca
     1201 ctccggcttc tcccgccacg gctctggcta gcaaagtctt gccagtcccg ggcggtccaa
     1261 ctagcaataa gcccttcggt aatttgcccc ctaatcttgt gtatttttta ggattcttta
     1321 aaaaatctac aacttctttt acttcttcaa cggcttcatc aacgccagcc acgtcggaaa
     1381 atttgacctg attctccggc ttttcggcat atattttagc tttattccgg ccgaaatcga
     1441 gcatcgggtt gctacttccc attcgtctga aaattacccc ccataaaccg ataaaaaaga
     1501 taataggaaa tacccagttc agaaaaaaat tacgaagcga attcgactcg tacctacctt
     1561 caaaattgac acccgaggct tgtaatttcg ccaacagttc cggatctgga agttccggaa
     1621 tgcggctgac gataaattgc cgggctacct gtttttcgat cgagggcact ctcaatcgcc
     1681 acggtgcagt cggggcatga gtaagcgctt gctcttcatt aatatcttcg gatttgaatt
     1741 cgcctacgat gcgatcctcg aaaattacga ttcgcgctac ttttccggaa tccaaagcat
     1801 ttaaaaagtc tctatagctc aagcggacat tagccggtct atccggaaaa aataacaact
     1861 gggtaaaaat tatcatcagt gtgattaacc agaagtaaat cagtgaaaat ttttcatttt
     1921 tcttcataac tgtctcacct catcagtttt caattgacac ttcatttatg aaaaatttaa
     1981 gtcatttccg ctaattgtgc cattataatt tctctcggta actcaggatt cataagatta
     2041 aggagttatg tcagcctgaa aacatattta attttttcag taggtgttat aaggcttgaa
     2101 tcaaaccgca ttttcttgaa attactttga aaattattaa atatcaaatt cttacgaata
     2161 tgctgaaaat gccagtaatt ttaaactaaa catctttttt gactcgcgaa aattcttgca
     2221 tttccagaaa taactcacta taatttatca cttttaaann nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnntc gcgaaaattc ttgcatttcc agaaataact cactataatt
     2401 tatcactttt aaagcgcgaa tcgacaacac ggaggaaaat ttatgttgaa aaagaagaac
     2461 tctcgaatgg ttaagattag ttccgtgtat taacatttat ccattcatca aaataccact
     2521 ggaagcctat cagggcaaaa caatgaccga aattgaacaa aaagaaggac ttcgcaagta
     2581 aattcagatt ttttatgaat tgaaagctgg aattaaagtt ggtttaattt cagctttctt
     2641 tttaaatcca attcgatggg taaaaagata aaaataaaat tggtaatttt ttccatctta
     2701 ttattttgga atttgttgct ttcccaagaa caccaaaaat ggacagacta ctgggaaata
     2761 cctttaaatt taccagaaaa acccggggaa atattttcta ccaacttatt ttcttcttcg
     2821 gctatagcct ggattaaaat ttaccaaaaa gtgattgcca gtcaggattt accatcgtgc
     2881 gtcttccacc cttcttgttc acgtttcgcc ttgacctcca ttcaggaatt tggcgttgtc
     2941 aagggcgctt tattagccag tgatcgcttg atgcgctgta acccatttgc tatgcgctat
     3001 tatccatttg acggcgaaaa attagccgat ccacctcaaa aatatcgttt aaaatagacc
     3061 agagccattg tgaaaaaatc tattttattt cttctcattc tttcgatttc aacaactgcc
     3121 aatgctcaga tagaatcttc tctttttgaa ccggcacact taaaagaatt tggactttac
     3181 ctatttcagc aaaaagatta cctgcgagcg gcgatggaac ttgaacgtta tcgctatttt
     3241 gcaaaaagtg gcgatgattc gattactttt gttattgggc tgtgccatca attccgccag
     3301 cgttatgatt ttgccgccgc aacttttcgg gaactggctg aggatgattc cagtaaattt
     3361 aaagaatctg cgcgcttagc tatgttatat aattatacaa aattagaaga ttggcctaaa
     3421 atcctgggat taagctatca aaatgatgcc gagttttatt tctattattt ggccagcgtg
     3481 aaaacagctc caactacaat taccacttcc ttttttcaaa atgttaatga tgaaagtctg
     3541 cgcaatcaac ttaaagcact tgaacaggat cgtaataaaa ttcaccccaa atcacctttt
     3601 ttagccggaa cattatcaac tcttatcccc ggattaggaa aagtttacat caatcggtcc
     3661 ggcgatgcgc tcttttcgtt cggaatgatc aatctctttg cgcttttgac ttataaagcc
     3721 tttcaagccg aactgcctgt aactggcgtt ctgtcaggtg gattgactct ctcgctttat
     3781 ctcggaacaa tctacggcag ttatgtcggg gctgaacttt ataatcgtaa cttagatgcc
     3841 gattggcaaa gtaaaatgac tgaactgaat ccggttgatc ggcaccctta ttggtccaca
     3901 tggtaaaaag aataattctt ttactacttg cgatcttctc ggtaattttt tctacggaaa
     3961 atctcggcga ttactataat gccaaacaac attatttcga agccattact gagtacaaac
     4021 ggcaactatt ttttggccag tacgaaagcc aagatgaaat tttactaaaa atggcaaagg
     4081 tataccacgc cgttcagcag gatttattcg ccgaagaaat acttttacaa gccatctcca
     4141 atgatgaggc gtccgctttt gactgggaat gttcactcga attagctcgt ctgcattggg
     4201 ataattataa ctatttcgaa atgcgcaatg tcttggattt tattagtata aattcaaagc
     4261 aaccaattca ggatacaatc aattatatca aagcctggac ttacttttat gaagccaagt
     4321 ggggcgaagg tctggctttg ctagattcgg tcgattttat tgactcaaaa tcattgcaga
     4381 aagaaatttc cggtgttcat aaaattcctc aaaaatcgcg cactctggcg ctggtaatgt
     4441 caaatatcat ccccggcacc gggcatttgt acgcgcacga ttaccaaaat gccctttact
     4501 cttctctttt ggttggcgga acgatggttt cgatcatttc gaatgtcatc gatcgagcct
     4561 attttgttgc cttgattaaa tatctattta tctattcccg ttataataat ggaacattgg
     4621 ttaacctcgc caaaaaagta gatagagata atattgatgc gattggtaat tatctaaaaa
     4681 ctgtctcgca gaaatatcca gcgcctctgg aattgctgga gtgtttgcga tgatataaaa
     4741 gccttttaac cacaaaggat ctaaagcacg attaataagg ttgagggtga gagaagtcat
     4801 tggtcattgg tcattagtca ttgggcatta gtcattagta aaaaggctga ggctaagaaa
     4861 aaaagctata ctgtcactct gagcctgtcg aagagggaca ggatcgaata ccctatgcta
     4921 cctgtgtgct tcatccttcg acgagctcat ccttcgacaa gctcaggatg acaggaagaa
     4981 tgtcactctg agccatagtg tcactctgag cctgtcgaag tgtgatttac tatgtcatgc
     5041 tgagtttatc gaagcatgag tctaccgtat catggcgatt attttgtacc aacgcccaag
     5101 ccttgaaact agataaacga taaccttctt aaagtccact tgaagtccaa aaatatccat
     5161 taaaagcttg aattaattat aaatattcgt aatattataa aaagaaaata gcatggcagg
     5221 aagtttctct tataaaatta ctttgaggct cggcaggaaa gtgaatagtg ctgtccacat
     5281 accttacttg cctgaagata gtctgatgaa aagtttaacg aattattgag gtggtatgtt
     5341 atgaaaagat attttgtttc cttagttgtt tttaccctgg caggattttt agcctatgcc
     5401 caggaattgc cggcgaaccg tagttcgcta aattcaccat tgcataattc aggctattct
     5461 gaagaaatct tagggaaaag cgagcagttt aaccggcatc cttattctaa atttctgatg
     5521 gacttagatg aacaacctta tagacatccc tgtccttctg agtctcgaga tgctacttct
     5581 actctcaatc taaataatac cgccactact aataaggaac cagaatggat aagttattat
     5641 gcctctggac ttcttccatc tccagatata gcgactagta ttgccgttga taaacatggt
     5701 aatgtctatg ttactggtta tagcgtagag gcactgtttg gagcagatta ttgtactata
     5761 aaatatgata attctggtaa tccaatctgg gcggttagat atgatggcgg tggagatgac
     5821 tgtgcctacg ctcttgttat tgatggctct tgtaatgttt atattacggg atatagtgaa
     5881 ggttcagaaa caggtctaga ttatactact gtaaaatata actcaaatgg tgaagaacag
     5941 tgggtagcta gatatattgg gccagaaaat ttagatgaaa gagcattcgc tcttgtgatt
     6001 gatgattctg gtaatgttta tgttaccgga tatagttata gtccaagaac aaggtctgat
     6061 tatacgactg taaagtataa ttcaaatggc gaagaacagt gggtagccat atataatgga
     6121 ccagtaaata atgaagactg tgccaaggct attgctattg ataattctgg caatgtttat
     6181 gttactggag gaagctatgg ttcaggaaca gtatctgatt atgctaccgt gaagtacaat
     6241 tccaatggtg aagagcagtg ggtggtcaga tatgatgggc caggaaatga ttgggatgaa
     6301 gcaaatgctc ttgcgattga tagttctggc aatgtttata ttacaggatt tagttatggt
     6361 tcagaaacgg agaatgacta tgcgacggtg aagtacgatc aaaatggtga agagcagtgg
     6421 gtaacaagat ataatggttc agaaaattct catgacaagg ggaatgcttt agtgattgat
     6481 ggtgctggga atgtttacgt taccggatat agtaatggtt caataacagg ggcagattat
     6541 gcgactgtga agtacaactc aaatggtgaa gagcagtggg taacaagata taatgggcca
     6601 ggaaattctg atgacgagtc ttatgctctt gctattgacg gttctggaaa tgtttatgtt
     6661 accggatata gctacggttc aggaacagta actgattatg ctaccgtaaa atacaactca
     6721 aatggtgaag agcaatgggt agctagatat aatggtccag aaagtgatta ttatgattat
     6781 gactatgcct acgctattac ggttgatgac tctggtaatg tttatgctac cggatacagt
     6841 catagcttag gaacagttga agattatgca actgtaaaat acaattcaaa tggtgaagaa
     6901 cagtgggtag ctagatataa tggtccaggc aattctgaag acaaggctcg tgatattgcg
     6961 attgatgggt ctggcaatgt ttatgtttcg ggaagtagct attgttcaca aacagggtat
     7021 gattatgcga ctgtgaagta caactcaaat ggcgaagagc agtggacagt cagatataat
     7081 ggaccaggta attctgatga cgagtcctat gctcttgcta ttgacggttc tggaaatgtt
     7141 tatgttaccg gatatagcta cggttcagga acagcaactg attatgcgac cgtaaaatac
     7201 aactcaaatg gtgaagagca gtgggtagct agatataatg gtccaggaaa ttctgctgac
     7261 gaagcctatt ctatggttat tgatgggtat ggcaatgttt acattactgg attcagttat
     7321 ggttcaacaa cagaggcaga ggcagattat gcgactgtga aatataattc aaatggagaa
     7381 gaagtttggg tatcaagata taatggacca agaaattttg acgacgaagc ctattctata
     7441 gttattgatg ggtctggcaa tgtttacgtt accggattta gttatggttc aataacaggg
     7501 gcagattatg cgactgtgaa gtataactcc gacggacagg agcagtgggt agccagatac
     7561 gatggaccag gaaattttga cgacgaagcc tattctatag ttattgatgg gtctggcaat
     7621 gtttacgtta ccggatttag ttatggttca ataacagggg cagattatgc gactgtgaaa
     7681 tatgattcaa atggtaaaga gcaatgggta gtcagatacg atgggataga taattcttct
     7741 gactatggca aggctattgc tattgatggg tccggcaatg ttattgtcac cggatatagt
     7801 gatagtttag gaacaaggtc cgattatacg actataaagt ataattcaag tggtgaagaa
     7861 cagtggatag ccaggtataa tggaccagga aatgattatg acaaggctta taatcttgct
     7921 atagatgatt atggaaatgt ttacgttact ggagaaagca gagatttaaa aaaaggttat
     7981 gattatgcta ctgtgaaata cgattcaaat ggtgaagagc aatgggtagc ccgatataat
     8041 ggaccaggaa atgatgatga taaagctttc gcttttgcta ttaatagttc tggcaatgtt
     8101 tatgttacgg gaattagtga tggtgcaggt tggagcgttt ataccacgat taagtatagc
     8161 tcggatggaa ataggattga tgaagaaaaa ctaaaaacgc cggtttattc tctatcccag
     8221 aactatccca atccgttcaa ctcagctact actataacct attcgatcag ttcacagtca
     8281 ctagttaaga tcgaggtcta caatattatt ggtcagaaag tactcacgct tgaagatggt
     8341 attaagactc caggagttta tactgcgagg ttcagtcctc gcgcagattt taccagtggg
     8401 atatattttt accggttaga cgttctggat atgagtaata agagcaagcg ctttagcgaa
     8461 atcaggaaga tggtgttaat taagtgaaga ataggcttga tgttcttggt cgctagcgtt
     8521 attaatttta actgctcgtg gtttagtaga tttagtgtcc ctctaaaaaa tgtcaccctg
     8581 agtttatcga agggtgagtt tatcgaaggg tgagtttann nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnntg agtttatcga agggtgagtt tatcgaaggg tgagtttacc
     8761 gaagcattgc gatttattta gcaccaacgc tcaagtactg gccagaaaag gaacaatgcc
     8821 aagttcaacc aacagccaca tagtaacgtg ctatttgtag aaaaagaagt gaaaccaaaa
     8881 attaagttag gtgtacctca ttagtcttag agcatcagaa tacatagtga aattattaaa
     8941 cataacataa ttgagttctt gatcacatag ggcgagcact ttttctaatt cttgatcgct
     9001 aaaagtataa ttataatcta atctattccc gatgtgtaat ccgtgtaaac gataatagcg
     9061 gtaatcgcca taataactgg gttgatataa tggatcaacg caatgaatca ggtttaattc
     9121 ccgacatact ttttggatcg ttttcttgtc ccatttggga ttgcggactt ccagaatgaa
     9181 ttcgaaatct tcactgtctt ttctaatctt ctcgaaaaaa ttatacaggt tcagcaaata
     9241 tttctcatct ggagtaaaac ttggtggaca ttggaataga atcttagtgc aacccaattt
     9301 caaggcaaat tctcgagttc tctcccaggc ggtaaagacc tcatcggtat ctttgaacaa
     9361 tccgtaattc tctttagccc caattttttc tctcagtcgg cgataagtga acgaaccagc
     9421 cggatgagta atgatttgcc aggctttaat gacgaactcg aatttcggat tctgcttttc
     9481 ggccgccaga cgccattttt cggcggtgac aatttgaggc aattgataaa aggtgatgtt
     9541 aatttcgata caattgaatt cacggtaata ttgctctttt tctatcggaa agccgcaaca
     9601 accgattttg aattctttca tatctaaaat gtagtcaaat ccttactaaa aagtgacaaa
     9661 gaatttctaa gttcgccaaa agccttttct ctgcaagatc acgattttca aaatcctccc
     9721 cttagccgac aatgaaaata ttggtttttc aggttaaata ttagtaatta atttaacgaa
     9781 aattaataat taaacctttg tgccttcgtg tcttagtggt aagaaaactt agattacttc
     9841 caggattttt tcttcgatat tttttttagg ttgggcgccg gtaatctgag ccattatttt
     9901 accatttttg aaaatcagca gagtcggaat actgcggatg ccgaatttct gagcaatttg
     9961 cggggaattt tcgacattca ctttgccaaa tttcattttg ccgttatact tttgcgccag
    10021 ttcctcaatt accggcatca gtttcaaaca aggagcgcac caggttgccc aaaaatcaac
    10081 cagcaccggt aaatcggatt gctcgacttc agcattgaaa tttgattgag tgaattctac
    10141 cataaaaccc cgctttttta ctacttgatt atctcaacgt ttcacgctat aatatattct
    10201 taactacttt taatctcaaa tctatttttt ccggaaattt tttatctggc gcgcacccat
    10261 tgccatat
//
LOCUS       DAOMJU010000077        10077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6874_length_10077_cov_22.537019, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000077 DAOMJU010000000
VERSION     DAOMJU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6874_length_10077_cov_22.537019"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PLO58_07555"
     CDS             complement(<1..569)
                     /locus_tag="PLO58_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQO74681.1"
                     /translation="MNFIWLGLMLIALIVGALNGRIELVMKAAFDNAAAAVEIALGLI
                     GIMALWLGIMKIAEKGGIIKILSRAIRPIAKFLFPSIPSDHPAIGSMLMNMIANWLGL
                     GNAATPLGLKAMEELQSLNQFKDTATNDMVTFLALNTGTICLIPMTVIAVRAQLGSAN
                     PFEIIGTTIISSTCATIAGVTAAKLFQRLP"
     gene            complement(891..1451)
                     /locus_tag="PLO58_07560"
     CDS             complement(891..1451)
                     /locus_tag="PLO58_07560"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQO74682.1"
                     /translation="MDFEEKCINSKRVFNGRLLQVYRDEVRLPNGKISNREWIKHPGA
                     AVVIPYLGNGQILLIKQFRYPVRQIMLELPAGKIDPDENPEQTIRRELAEETGYSAQK
                     LTKLGLIHTCVGYSNECLHLFWADELQKCDTRPDDDELIEIIPMSINAAMNLLYQGKI
                     TDAKTIIGLFWADNIINRRYLNLCSD"
     gene            1892..2404
                     /locus_tag="PLO58_07565"
     CDS             1892..2404
                     /locus_tag="PLO58_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQO74683.1"
                     /translation="MKVRINSLKYGKQMIRAALGGDELDVADLDLSAPVAVMLNIDRG
                     TTEIKIDGKVESIARFDCDRCLESFSMPISGTFAIIATYSESNVKIDDENVIQLSSMT
                     NEIDITTHIHDTLLLSIPMKILCEEDCQGLCPICGANHNKTECHCNVAEPDARWDSLK
                     KLTIKITEDQ"
     gene            2406..2588
                     /gene="rpmF"
                     /locus_tag="PLO58_07570"
     CDS             2406..2588
                     /gene="rpmF"
                     /locus_tag="PLO58_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQO74684.1"
                     /translation="MPQPKRRASKSRRDKRRTHQVLTEATVVKCPQCKEPKLPHRACP
                     NCGYYRGRPVIIPKEK"
     gene            2690..3697
                     /locus_tag="PLO58_07575"
     CDS             2690..3697
                     /locus_tag="PLO58_07575"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQO74685.1"
                     /translation="MKKPKSTITAFGMYVPERILTNADLEKMVDTTDEWIRTRTGIRE
                     RHIAAEGEATSAMAIKAFQDLQRRFNVDPLTIDLIIVATISPDMFFPSTAALIQEGIG
                     AKNAYGFDLSAACSGFIYALANAAQFVENGTCRRVLVFGADTMSSITDYTNRDTCVLF
                     GDAAGVVLLEPTPPGDDSGIIDFILKMDGSGKDYLYMLGGGSLHPATAETVAKKMHYI
                     YQEGKTVFKFAVKSMADISLEVLERNGLTGKDIKLFIPHQANKRIIDASADRLKLSDE
                     QVMINIDRYANTTAATIPLALVEGFDQKRIKRGDLVLMAAFGGGFTWGSLLLRWGIPN
                     D"
     gene            3690..4616
                     /gene="fabD"
                     /locus_tag="PLO58_07580"
     CDS             3690..4616
                     /gene="fabD"
                     /locus_tag="PLO58_07580"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQO74686.1"
                     /translation="MTKVAFIFPGQASQFVGMGRDFYESSALARQYYDLAETRLGFPL
                     KKFSFEGPLSELTKTRITQPAIFVLSCIIFFELKARGYLPDMVAGHSLGEYSALVACG
                     ALTFEDGLDLVKIRAEQMQIASEQKPGTMAAIVGLDYDQVKKIISESNFSGICQIANC
                     NSPNQIVISGDVYKVQALMTDLKKAGAKMAVELSVGGAFHSDLMQPASEALKEALSKT
                     EFKTPDCPIYSNVTATATRDPQEIRNRLYQQLTSPVLWTDTIRNMIADGAEIFVEVGP
                     GKVLQGLVKRTSADVNVTGVSKFSELEQIAWN"
     assembly_gap    4704..4803
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4804..5508
                     /gene="fabG"
                     /locus_tag="PLO58_07585"
     CDS             <4804..5508
                     /gene="fabG"
                     /locus_tag="PLO58_07585"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HQO74687.1"
                     /translation="SGRGIGLTIAQTFAENGASVILSDYNQESLADAVALFKAKNFPF
                     DALPCDVTNSTEVQNLIDSIIQKYSRIDILINNAGVTRDAILLRMKEEQWDAVINTNL
                     KGVFLVCRAAAKYFLKQQSGKIINISSVVGLTGNLGQSNYAASKAGVIGFSKSLAKEM
                     ASRNITVNVIAPGFIDTAMTAALSDEVRTQFLRNIPLNRMGTPQEVANAALFLASPLA
                     DYITGQVLKVDGGMVM"
     gene            5528..5761
                     /gene="acpP"
                     /locus_tag="PLO58_07590"
     CDS             5528..5761
                     /gene="acpP"
                     /locus_tag="PLO58_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQO74688.1"
                     /translation="MSNFEKVQEIVAEKLGIEKSKITMEASFIDDLGADSLDTVELIM
                     KMEEDFGIEIPDEDAEKLKTVGDVVNYLDSKLK"
     gene            5770..7017
                     /gene="fabF"
                     /locus_tag="PLO58_07595"
     CDS             5770..7017
                     /gene="fabF"
                     /locus_tag="PLO58_07595"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQO74689.1"
                     /translation="MPKRVVVTGMGVISPVGSDVPSFWSNLLNGISGVDYIKSFDASG
                     YSTQIAAEVRDFDPLKYFTQKDINRMDRVTQFGSAAAKQAVYDSGLIESGFSAERVGV
                     IVGSGIGGISTLEAQHQILMTKGHRMVSPLFIPMMIPDIIPGQIAMDFGFCGPNYGVT
                     SACATGSHAIGVAYQHICNGTADVMITGGAEAAITPLAIAGFANMKALSRCNNAPQKA
                     SRPFDSKRDGFVVGEGSGIVVLEELEHALNRKAKIYAEIVGFGFSADAYHITAPHPEG
                     KGAIISMENAIRMSGLPKSAFGYINAHGTSTPLNDRNETIAIKAVFGKQAYQIHISST
                     KSMTGHLLGAAGGIEFITLALALQNQIIPPTINYEFPDPECDLNYTPNIAQKATLVAG
                     LSNNFGFGGHNAVLAAKVYKPEN"
     gene            7014..7751
                     /gene="rnc"
                     /locus_tag="PLO58_07600"
     CDS             7014..7751
                     /gene="rnc"
                     /locus_tag="PLO58_07600"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQO74690.1"
                     /translation="MKVSVKNWFRKISPIEKEIGYVFRNKALLEQALTHRSRSVNGEK
                     SYERLEFLGDAVLAFIVAEILFEKYPDKSEGELTMHRSALVNGNALARVGSKLGIEKA
                     VIVEKGLNLKNPPTLHKLLASIVESLIGAIYLDRGLRPARKFVQRWIIPSTAEGKVVS
                     NFNYKGRLFEYCQKNGRNLPVFKVLRVSGPDHAKKYEVAVYINGQKMGYGVGRQKRNA
                     EQKAAHEALQKLMPEHTVAGNQILKDS"
     gene            7982..9895
                     /locus_tag="PLO58_07605"
     CDS             7982..9895
                     /locus_tag="PLO58_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HQO74691.1"
                     /translation="MEVTQKIGLLDWAVVAIYFALIFGIAIYYYRRAGENTGEFFLSG
                     RNLPWWLAGTAMVATTFAADTPLAVTELVAKNGIAGNWLWWNLAIGAMLTVFFFARLW
                     RRAGIMTDVEFTEMRYSGKTAAALRGFRALYLGIFMNTIVMGWVHKAMEKIFHVTIPG
                     IDPFLLVAIVAGIVAIYASASGLLGTARTDSFQFVFAMTGCIILAVIVIRLPEVGGAV
                     KMKAQLAPQVLDFFPKVGRINGEGITGGVLSLSLGAFIAHIGLQWWSSWYPGAEPGGG
                     GYIAQRMMSAKNEKHSLLATLWFTIAHYTLRPWPWIIVALSALVILPRASSTDILQQE
                     NPVLYHQVVEAYKNPDLMKSNPLASTPEFQNFYEKYENTVDPGVMYPKMMLRYLPTGI
                     LGLLIATFLAAYMSTISSQLNWGTSYIINDFYRRFLRKKASEKHYVLVSRIAMLIMIV
                     FSLFVTKYFLTTISGAWEFIINASAGMGAVLILRWFWWRINAWSEIAAMIAPLIIYPI
                     ARYKFGLQSPITLYPVVLGTTIVWLLVTFLTKPEKDSTLTSFYRKVHPGGRGWKKIAE
                     QLPDVKGDSGFGRAFICWIFGVLLVYASLFGIGKIIFHEYLLAGVYFLVAGISVWIII
                     HNLNAVGWKTLKE"
BASE COUNT         2809 a         2036 c         2293 g         2839 t
ORIGIN      
        1 ggcaatcgct ggaaaagctt ggcggctgta acacccgcaa tcgtggcgca agttgatgaa
       61 ataattgtgg tgccaattat ttcgaatgga ttagctgaac cgagttgagc acgcaccgca
      121 ataacagtca ttggaatcag acagattgta ccggtattca gcgccaagaa ggttaccata
      181 tcattcgttg cggtatcttt gaattggttt agagactgta attcttccat agctttcaag
      241 cccagtgggg tggccgcatt gcctaatccc aaccagttgg ctatcatatt catcaacatt
      301 gaaccgatag ccggatgatc ggaaggaatg gaagggaata gaaatttggc tattggacgg
      361 attgcccgtg aaaggatttt tattataccg cctttttcgg caattttcat aatgccaagc
      421 cagagcgcca taattccaat taagcccagg gcgatttcaa ccgccgcggc cgcattatcg
      481 aatgccgctt tcataaccag ctcgatgcgt ccatttaagg cgccgacgat cagagcaatc
      541 aacatcaaac ctaaccagat aaaattcata ttagcctctt catttttcta attaaaaata
      601 aaatgacttt attgctatca aatagtggaa ataaacatca tcgttggtca taggaaatat
      661 tatgcaagcc ggatggatta agccattctt gaattgtcct caaggtttga ccatttgctt
      721 caaaaccaat caaaaaataa atacgtctca aggaagtcaa aaataaagta gctggattca
      781 gcctcattgg aaattaattt atagtcgaaa tggctgaaag tcaatgtagg aaagaattac
      841 agttttccca taagtgattt agttcgggga aaaattttat attttacggt ttagtcagaa
      901 catagattaa gatagcggcg attaattata ttatcagccc agaataaacc gataatagtt
      961 ttggcatcgg taattttgcc ctggtaaagg agattcatag ccgcattgat tgacatagga
     1021 ataatttcga ttaattcgtc atcatccggg cgtgtgtcac atttttggag ttcgtctgcc
     1081 caaaataagt gcagacattc attgctgtag ccaacgcagg tatgaattag gccgagttta
     1141 gtaagttttt gcgctgaata tccggtttct tcggctaatt cacgccggat ggtctgttcc
     1201 gggttttcgt ccggatcaat ttttccagct ggtagttcca gcattatttg cctgacggga
     1261 tagcgaaatt gctttattag aagaatctgg ccgtttccca gataaggaat gactactgcg
     1321 gctcccggat gcttgatcca ttcgcggttg gaaattttac cattaggcag gcgaacttca
     1381 tcgcgataaa cctgtaataa gcgcccatta aaaacacgtt tactattgat acatttttct
     1441 tcgaaatcca tatttttata cccaaaaatc ctttttataa ttttaccaaa aaatgacgat
     1501 ttttgataaa aatacgtcaa cctttttaaa gtatttctat gaaaatttag ccgtgaagat
     1561 aaaaggagag gaggcgttct attattaacg ggaggagttc ccaaggaagg agggaagttg
     1621 gatgaaactc atcctgaaat gggagtgatg tcccactcag tgaacgcctc ctctttaatc
     1681 ctttttcaaa tatcgtaaac tttaacaatt atttttcaat taagttccgg ctaaatttat
     1741 ttaatcacct ggaacaaagc aaggattatc aatgccggaa aattttattg aaatatgggg
     1801 aaataatcaa agttcaatag gtggtttctc ctcggaaaga ggttaaaatt cttgattatc
     1861 gtgttcgttt ccaataaatt tgcaagttat tatgaaagtt cgtattaatt cgctcaaata
     1921 tggaaagcag atgatcagag cagctctcgg gggcgatgag ctggatgtcg ctgaccttga
     1981 tctaagcgcc ccggtggcgg taatgctgaa cattgacagg ggcaccacag aaataaaaat
     2041 agatggtaaa gttgaatcca tcgcccgatt tgactgcgat cgttgtttgg aaagtttttc
     2101 gatgccaata agtggaactt tcgcaatcat tgctacctat tccgaatcta atgtgaaaat
     2161 agacgatgaa aatgttatcc aactttcatc aatgacaaat gaaattgaca taactaccca
     2221 tattcacgac actcttttat tgagtatacc gatgaaaatc ctctgcgaag aagattgtca
     2281 gggactttgc ccgatatgcg gtgctaatca taacaagact gaatgtcatt gcaatgtcgc
     2341 tgagcctgat gcccgctggg attcattaaa aaaactaacc ataaaaatta cggaggacca
     2401 ataaattgcc acaaccgaaa cgtagagcat caaaatcaag aagagataaa cgccggactc
     2461 atcaagtcct gaccgaagcc acggtcgtta agtgccctca atgtaaagaa cctaaattac
     2521 cgcaccgagc ctgccccaat tgtggctatt accgtgggcg gccagtaatt atacctaaag
     2581 agaaataggg ctttagctgg ttgaaatttt cacaagcagt ccgatggtat gccatatcct
     2641 gcgatttaac cgggtcagag acaagaatag aggttaagga aaatttctaa tgaaaaaacc
     2701 taaatctact attactgcgt ttgggatgta tgtacccgaa cgtattttaa ccaacgctga
     2761 cctggagaaa atggttgaca cgaccgatga atggatccgt actcggaccg ggattcgcga
     2821 acgtcacatt gctgccgaag gtgaggcgac ttcggctatg gctatcaaag cctttcagga
     2881 tcttcaacga cgctttaatg tcgatcctct gacgattgat ttgattattg tggctaccat
     2941 ttctcccgat atgttctttc catcaactgc cgccttgatt caagaaggca tcggggctaa
     3001 aaatgcttat ggattcgatc tctcggcggc ttgttcggga ttcatttatg ctctagctaa
     3061 tgccgcccaa ttcgtggaaa atggtacttg ccgccgagta ttggtgtttg gtgctgatac
     3121 aatgagtagc attaccgatt ataccaatcg cgatacctgt gtgcttttcg gagacgcggc
     3181 gggtgtggtt ttgcttgagc caactcctcc cggggacgac tcaggcatta ttgattttat
     3241 cttaaaaatg gatggatcgg gcaaagatta tttgtatatg ctgggtggtg gtagtttgca
     3301 tccagccaca gccgaaaccg tagctaagaa aatgcactat atttaccaag aaggtaaaac
     3361 tgtattcaaa ttcgctgtca agagtatggc tgatatctct cttgaggtac tggaacgcaa
     3421 tggtctgact ggtaaagaca tcaaattatt cattccccat caagccaata aacgaattat
     3481 agatgcttcg gctgatcgtt taaaattatc agatgaacag gtaatgatca atattgatcg
     3541 ttatgccaat accacggcgg caactatccc actggcgctg gttgagggat tcgatcagaa
     3601 acggatcaag cgcggagact tggttttgat ggcggctttc ggtgggggtt ttacctgggg
     3661 cagtcttctt ttacgctggg gcattccgaa tgactaaagt tgcctttata tttcccggtc
     3721 aggcttcgca atttgtcggg atgggtcgcg atttttatga aagcagtgct ctggcgcgcc
     3781 agtattatga cctagctgaa actcggctcg gttttccgtt aaaaaaattt tccttcgaag
     3841 gcccgctgag tgaattgacc aaaacccgca ttacccaacc ggcgattttc gtgttatctt
     3901 gtatcatatt tttcgaattg aaagcgcgcg gctacctccc tgatatggtt gccggacata
     3961 gtctcggcga atactcagcc ctggttgcct gcggcgctct gaccttcgag gatgggcttg
     4021 atctggtaaa aatacgggct gaacagatgc agattgcctc ggagcaaaaa cccggaacaa
     4081 tggccgctat tgttggtctg gattatgacc aggttaagaa gataatcagt gaatctaatt
     4141 tttctggcat ttgtcagatt gctaattgta attctcctaa ccaaatagta atcagcggcg
     4201 atgtttacaa agtacaggct ttaatgactg atttaaaaaa agccggtgct aaaatggctg
     4261 tggagttatc agtcggcgga gcctttcatt ccgatttaat gcaaccagcc agtgaagccc
     4321 tgaaggaagc tctgtctaaa accgagttca aaacgccgga ttgtccaatt tattccaatg
     4381 tcactgctac agctacgcgc gatccccagg aaatccgtaa ccgactctac cagcaattga
     4441 cttccccagt gctctggact gacacgatca ggaatatgat agccgatgga gcggaaatct
     4501 tcgtggaggt tggacccggg aaagttctac agggattagt caaacggacc tcggctgatg
     4561 ttaatgtaac aggcgtttct aaattcagcg aattggagca gatcgcatgg aattaacttt
     4621 caaagataaa atagtgattg tcactggttc ggggagaggg ataggtttga ccatcgctca
     4681 gacttttgcc gagaatggcg ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnntcgggga gagggatagg tttgaccatc gctcagactt ttgccgagaa tggcgccagc
     4861 gtgattttaa gcgattacaa tcaggaatca ttggctgacg ccgtcgccct ttttaaggca
     4921 aaaaatttcc cgtttgatgc tcttccctgt gatgtaacca actctactga agtacaaaat
     4981 ttaattgata gcattattca gaaatattct cgaattgaca ttttaatcaa taacgctggg
     5041 gtaacccgcg acgccatcct tttgcgaatg aaggaagagc aatgggacgc agttatcaat
     5101 accaatttaa aaggcgtttt tctggtttgc cgagcggccg caaagtattt tctaaaacag
     5161 caatcgggca aaatcatcaa cattagctcg gtagtgggct tgactggcaa cctcggtcaa
     5221 tctaattatg cggccagtaa agccggtgtg atcggctttt ccaaatcgct ggctaaagaa
     5281 atggcttctc ggaatataac ggtcaatgtc atcgcgcctg gattcattga cacggctatg
     5341 acagccgctt tgtcggatga agttcgaacg caatttttaa ggaatattcc tttgaataga
     5401 atggggacgc cgcaagaagt ggccaatgcg gcgctatttt tagcttcgcc gctggctgat
     5461 tatatcaccg gacaggtgct taaagttgac ggcggtatgg taatgtaaac taacttagga
     5521 ggatatgatg agtaacttcg aaaaagttca agaaatcgtg gctgaaaaat tagggatcga
     5581 aaaatccaaa attacgatgg aagcttcttt cattgacgat cttggagccg attcactgga
     5641 cacggtggag ctgattatga agatggaaga ggatttcggc attgaaattc ctgacgaaga
     5701 tgctgaaaaa ctaaaaacag tcggtgatgt tgtcaattat ctcgactcca aattaaaata
     5761 accccgtcta tgcccaaacg tgtagttgtg accggaatgg gcgtcatttc cccggttgga
     5821 agtgacgtcc catctttctg gtcgaatttg ctaaatggca tcagcggcgt cgattacatt
     5881 aagtcttttg atgccagcgg ctattccact cagattgccg ccgaggtacg cgattttgat
     5941 cctctgaagt acttcactca aaaggatatc aatcgaatgg atcgcgttac tcaattcgga
     6001 tcggcggccg ccaaacaggc tgtatatgac tcaggattaa ttgaatctgg tttctcagcc
     6061 gaacgagtag gagtaatcgt cggatcgggc attggtggaa tcagtaccct ggaagcccag
     6121 catcaaattc tgatgactaa gggtcatcgc atggtaagtc cactctttat cccgatgatg
     6181 attccggata taattccggg ccaaattgcg atggactttg gtttctgcgg ccctaactat
     6241 ggtgtgactt ctgcttgtgc cactggttca catgctattg gagtcgccta tcaacacatc
     6301 tgtaatggaa ctgctgacgt aatgataacc ggtggtgcag aagccgctat aacaccgctg
     6361 gcaatcgctg gtttcgccaa tatgaaagcc ctctcacgat gtaataatgc accacaaaaa
     6421 gccagccggc cattcgatag caaacgtgat ggcttcgtag taggtgaagg atccggcatc
     6481 gtagtcctcg aagaattgga acatgccctg aaccggaagg ctaaaattta tgccgaaatc
     6541 gtcggcttcg gtttctcagc cgatgcctat catatcaccg ctcctcatcc agaaggcaag
     6601 ggagcgatta tttccatgga aaacgccatc cgaatgtcag gtttgccgaa atcagcattc
     6661 ggctacatca atgcccacgg tacctccacc ccgttaaatg atcgtaacga aacgatcgcc
     6721 atcaaagctg ttttcggcaa gcaggcttac cagattcata tcagttctac caaatcaatg
     6781 accgggcatc ttttaggtgc ggctggcgga atcgaattca tcactctggc gcttgcactc
     6841 cagaaccaga ttataccgcc cactattaat tatgaatttc ccgacccgga gtgcgattta
     6901 aactatacgc ccaacattgc tcagaaagca accctagtag ctggattaag taacaatttt
     6961 ggttttgggg ggcacaatgc cgtcctggcc gccaaagttt acaagccgga aaattgaaag
     7021 tatcggtaaa aaattggttt cgcaagattt cgccaattga gaaggaaata ggatacgttt
     7081 tccgcaataa agctttgttg gaacaggctc ttactcaccg ctcgcgttcg gtgaatggcg
     7141 aaaaatccta tgagcgatta gaatttctcg gcgatgccgt tctggctttt atcgtcgccg
     7201 agattttatt tgaaaaatac ccggataaaa gtgagggcga actgacgatg catcgctcag
     7261 ctctcgtaaa tggaaatgcc ctggcacgcg ttggtagtaa attgggaatc gaaaaagcag
     7321 tcatcgtcga aaagggttta aatctcaaaa atccgccgac tctgcataaa ttacttgcca
     7381 gtattgtcga atctttaatc ggtgcaatct atctcgaccg tggactgcgc ccagcccgca
     7441 agttcgttca acgctggatt ataccatcaa cagccgaagg caaagtcgtc agtaatttta
     7501 attacaaggg acggcttttc gaatattgcc agaaaaatgg acgcaactta ccggttttta
     7561 aagtactaag agtctctggg ccggatcatg ccaagaaata tgaagttgcg gtctatatca
     7621 atggtcaaaa aatgggatat ggcgttggtc gtcagaaacg caatgccgaa cagaaagccg
     7681 ctcatgaagc tctgcaaaaa ttgatgcctg aacacactgt tgccggaaat cagattttaa
     7741 aagattcata atattatttt gaaaatccgg ttgaatttcg cgattgacat ttaaatattt
     7801 ggtaattatt cagaaatggt acagtaagcc tagtcaatat ttagacctaa acatttttga
     7861 tttaagcaaa ttcgatttgt accaatgatg tttccttcga ttgataagcc ttcaaagaat
     7921 ttagtaatat cgaattaact acttaattat tagttattaa taaggttaat gagaggtaaa
     7981 aatggaagtg acacagaaaa tcggattgtt agattgggcc gtagtggcaa tatattttgc
     8041 gctaattttt ggcattgcaa tttattatta ccggcgggct ggggaaaaca ctggtgaatt
     8101 ttttctctcc ggaaggaatt taccttggtg gttagccgga actgccatgg tagcgacaac
     8161 ctttgcggct gatacacctt tagctgttac ggagctcgtc gctaagaatg gtatcgccgg
     8221 taactggcta tggtggaatc tggctatcgg tgcgatgctg acggtctttt tctttgctcg
     8281 actatggcgc cgagcaggaa ttatgactga tgtagaattt actgagatgc ggtattccgg
     8341 caaaacggcg gcggctctgc gcggattccg agccctatat ctgggtattt ttatgaatac
     8401 aattgttatg ggatgggtac ataaagccat ggagaaaata ttccatgtca cgatcccagg
     8461 gattgacccg tttcttttag tagcaattgt cgctggtatt gtcgcaattt acgcttcagc
     8521 ttccggcctg cttgggacgg ctcgtaccga cagtttccag tttgtatttg ctatgactgg
     8581 ttgcattata ttggctgtta tagtcatccg actgccagag gttggtggag ccgttaaaat
     8641 gaaagctcaa ttggcaccgc aggttcttga tttctttccc aaagtggggc ggattaacgg
     8701 agagggaatt accggtgggg tactatctct atcattgggt gcctttattg cccatatcgg
     8761 actgcaatgg tggtcgtcct ggtatccagg ggctgaacca ggcggcggcg gttacattgc
     8821 gcaacgcatg atgtccgcta agaatgaaaa acacagcctt ttggcaactc tctggttcac
     8881 aatagcgcat tatactttac gtccatggcc ttggattatt gtagctcttt cggcattggt
     8941 gattctaccg cgagcctcaa gtaccgatat acttcagcaa gaaaatccgg tactttacca
     9001 tcaggtcgtt gaagcgtata aaaatcctga tcttatgaaa tctaatccgc ttgcttctac
     9061 cccggaattt caaaattttt acgaaaaata tgaaaatacc gtcgatcccg gagttatgta
     9121 tcctaagatg atgttacgtt acttacctac aggaatttta ggtctattga ttgcgacttt
     9181 tttagccgct tatatgtcaa ctatctcttc ccaattaaac tggggcacat catatattat
     9241 aaatgatttt taccgaagat tcctgcgtaa aaaggctagt gaaaaacatt atgtattggt
     9301 ctcccgaatt gcaatgctga ttatgattgt attttcatta ttcgtcacga aatatttcct
     9361 gactactata tcaggagcat gggaatttat catcaacgct agtgcgggga tgggtgcagt
     9421 tctcatttta cgctggtttt ggtggcgtat caatgcctgg tcggaaattg cggctatgat
     9481 tgcaccacta attatctatc caattgcacg gtataaattc ggactgcagt ccccgataac
     9541 gctttatccg gtagtcctag gtactacgat tgtttggcta ctggtgactt ttttaaccaa
     9601 accggaaaaa gactctactc ttaccagctt ttatcgtaag gtgcatcctg gtggtcgcgg
     9661 ttggaagaaa attgctgaac aattacctga tgtcaagggc gatagtggtt tcggccgagc
     9721 atttatctgc tggatttttg gggttttgct ggtctatgcc agcctgtttg gaataggaaa
     9781 aatcattttt cacgaatatc ttttagcagg agtgtatttt ttagtggctg ggatcagtgt
     9841 ctggattata atccataatt tgaatgcagt gggttggaaa acgttgaaag aatagaaatc
     9901 tgaaaataat tatcatttcg gaaattatct atgtagggta tatttttaat gtaggagaaa
     9961 aatatggagt gtcgtcaggt acaaaataaa aggaattgca attgtagcta tgaaccctgt
    10021 aatcgaaaag gtgtttgttg tgattgcgtt cgttatcatc tgcggtcgag gcaacta
//
LOCUS       DAOMJU010000078        10012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6935_length_10012_cov_20.543738, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000078 DAOMJU010000000
VERSION     DAOMJU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6935_length_10012_cov_20.543738"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..656)
                     /locus_tag="PLO58_07610"
     CDS             complement(18..656)
                     /locus_tag="PLO58_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQO74692.1"
                     /translation="MSSSEKKMNIIAIGSHPDDIEIGCGASLAKFNAKGHSVYLYVAT
                     LGGAGGDPDVRRAEQENSARIMGVKDIFWGSFEDTDITINKNSIAALEEIIHKVTPDM
                     ILVNYHDDTHQDHRNIAAITNSATRYIPNVLFYETPTTQNFMPNVFVDIGTEYLAIKL
                     RALLAHTSQINRTNIANLSIIQVAESSAHFRGVQARVACAEAFHSLRLFIDI"
     gene            complement(653..1351)
                     /locus_tag="PLO58_07615"
     CDS             complement(653..1351)
                     /locus_tag="PLO58_07615"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HQO74693.1"
                     /translation="MICAIHQPQTYPWLGYFAKIMQADIFIFLDNVQFKKNEWQNRNK
                     IRTPQGWIWLTVPVIHHFGQKIDEVKINNTVNWRKKHLQTLQTYYGRAPFFKLYFEKL
                     ENLYQFEWQNLAQFNITAIRLLMSLIGIDTPTVIASEIKALNQNSQISAAERLIIATK
                     AVGADCYLSGIGGCNYLQPELFAKNDIKLKFQSFEHPVYRQMTNDFLPNMSILDLLFN
                     EGQRANSIIQGGIR"
     gene            1419..1604
                     /locus_tag="PLO58_07620"
     CDS             1419..1604
                     /locus_tag="PLO58_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74694.1"
                     /translation="MDTIIKGCIIVGVISLILGIVSRLTLKPFFVEAQAFMQFAQFCF
                     LAAITFLLYKIAYKVKD"
     gene            complement(1616..3193)
                     /locus_tag="PLO58_07625"
     CDS             complement(1616..3193)
                     /locus_tag="PLO58_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74695.1"
                     /translation="MLIKRVTRICLNKKFAPFLITVSLLIMTDSLVFSQTKPGYILLK
                     TGVHFDSQVSGGKYSLAELANIIARHKLDVGIITDHDNMKVSYGIRPFENFLKFTIEE
                     NSVHRYGVEKYINEINALDAAYPNLILIPGIEAVPYYRWEGSPLYENMVLRDWHTHLI
                     VFGLEDPKIFEQLPSLANHNIGYSKPDGKIIKYIGENFTHFALMTLYFLLFLISLALI
                     FRRKYGLFSRKNINRPRQNIRFSFPAFIMAIIFGLILYNQYPFLPRKYTPYDRKAGSA
                     PYQELIDYVNAHNGLIFWAHPEVTHNEVRQINIPLMNQAIKISTEAYPHRIMETQDYT
                     GFAIFWEGAKVIGRPGGLWDIALAEYCAGLRRKPVWAIGELDFEDSSNLNNVTETLTF
                     LFVKERSRAGVYEAMRAGRMYATRSFTGDKIVIDDFSVYDLHTGNNAFIGETLTHAQA
                     PVAIHIRLRALTTLNKPETIFLYRNSELIKIFYLNTVLDEWFVDESPMSNPMCYYRIY
                     GGDSWPTIASNPIFVSK"
     gene            complement(3156..>3509)
                     /locus_tag="PLO58_07630"
     CDS             complement(3156..>3509)
                     /locus_tag="PLO58_07630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQO74696.1"
                     /translation="YRTDVPEIMASSDIFVFTSLNEGFGNVIIEAMACGLPIIGTRVG
                     GVPSLIQDGVNGLLVNSDDAEALATAIQRIASNPEQRQSMASTNRKKAEYFTIDNYIK
                     RVIYAYQTCNTNLPE"
     assembly_gap    3510..3609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3610..4097)
                     /locus_tag="PLO58_07635"
     CDS             complement(<3610..4097)
                     /locus_tag="PLO58_07635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO74697.1"
                     /translation="MVKNGYEVLLVAGRTTHPPFDLTEYARLNGFQLYFLRTLVRDPS
                     PIYDPLAFLQIYRLILKYRPEILHTNTSKAGFLGRLAGRLARVPKILHSTHGHIFYGY
                     YNRYISKCFITLEKFSAHYCDRVLNLTEIGRQDHIRERIGPSSKFYVTSGGADLDPFF
                     KA"
     gene            complement(4189..5391)
                     /locus_tag="PLO58_07640"
     CDS             complement(4189..5391)
                     /locus_tag="PLO58_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQO74698.1"
                     /translation="MSILHIPTAGTPYNFTEIARATLGLFESDHVQDNFSQTLKKYFD
                     VENLALVNSGTTALFILLELFKKIRTSPKRNEIILPAYTAPVLLLPIRAAGLNAVLVD
                     VDLTTFNMDMHKILNAVTPQTLAIMPVHMFGLPMDVAPINESIAGQDIFIVEDAASSL
                     GSTIGGRQTGSIAPFGFYSLNRGKNVSTLAGGIIVWKDQRYSPIIEELVAGLPPVSQR
                     AEFLMWLNFLGLSIAVNPLCYTLLLPFIAKFKYTTLHPHFDSFAYTNMQAALGKTLWR
                     RIDQLTKRRVENGKALMQIFTGKPGYTIPKILPNSQAAFNQFPVIVPNLDQRQRLQKK
                     LLKVGIETTRLYEKPLHHFFPELNPFTYDNYPQATYLAERLLLIPTHAQIGPAIRQKI
                     NSVVEALN"
     gene            complement(5393..6019)
                     /locus_tag="PLO58_07645"
     CDS             complement(5393..6019)
                     /locus_tag="PLO58_07645"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQO74699.1"
                     /translation="MTTLNLKNQTRTAAFQTLTSIRVRTAALKIARQINGRFLDVGCG
                     NGLFLLEYYVTDAKKAIAFGLDHDRQAIYEARTLFKDNNISIEPLLIGDGFNLPFGDE
                     KFEAVFCLNTFLNFHPFEKIEAFIIEMHRVCKKGGWIVFDYRNSRNPYLWFRYRLSSL
                     TGRLNVHGHRRKEFQPLMRRLNIKNSEQMAIPSPLPGFPLGYLNKWEK"
     gene            complement(6016..7173)
                     /locus_tag="PLO58_07650"
     CDS             complement(6016..7173)
                     /locus_tag="PLO58_07650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQO74700.1"
                     /translation="MRNQNTLLIPGDFPPVVSGISTYYYQIWRLLPQENNYILCPKDP
                     GWEQFDSQTGLQTIRRRIPAGNLIRDKLLKGLLYAFWTLVLDRKYHFRKIHCGQVLSS
                     GFTGWLMNKLFGIPYNIYVYGSETFRFGYSPLFMKYIKTFLSNAEYIVPNSQFTCNEF
                     MALGIPPEKFKIITPGVDTGFFKPAPKDATLMEKYGLNGKTVLLTVARLDERKGHEVM
                     IRAVADLSSQFPDLIYLIVGAGREEERLRKLTKELNVTDQVIFCGYIPDAELPKFYNL
                     CDIFILLNRQTYDDVRLRGDYEGFGIVFLEAAACGKPVVAGDFGGVASAIENGRSGLI
                     IDANDQSAIRQTLVSLIQNPSLREQLGQYGLERARQSFDWQIISREIMKIL"
     gene            complement(7166..8098)
                     /locus_tag="PLO58_07655"
     CDS             complement(7166..8098)
                     /locus_tag="PLO58_07655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO74701.1"
                     /translation="MIISKPYTGNISRPFVKYPKGASEGRIEIHRPENVTEPLISIII
                     PTMGAARQEFLPGLMSQIERQTFRNFEVILIIGDTRQGRAINCGAALAAGKYLVIFDD
                     DTRLGSDDLLAKMLAQMENHPDIGLAGVANLIPENANWFVRRVMRELPRRTSPIVSEI
                     TDSDLAEHPCCIIPRDVFIKIGGENELIPRGLDPYLRQEIRQAGYRVVVLPELYIHHL
                     PPSTFSKFVRQFYRNGKMAAYVNKFYPEFVVELAYQHGTEVSQKRSTIVRIGSYVWRL
                     CKTIVTFRAFYLLSLVFYLSGYLIGYITLGEQDA"
     gene            complement(8141..>10012)
                     /locus_tag="PLO58_07660"
     CDS             complement(8141..>10012)
                     /locus_tag="PLO58_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HQO74702.1"
                     /translation="SDYPISAVDIKNVEINDAFWLPKIKIIQDTTIQYAFKKCEEEGR
                     MENFLIAGGRKKGPVRGKMPFDDTDVYKIIEGASYSLISRPNPALAAYLDSIISIIKI
                     GQEPDGYLTTWFTIDRMHPPAWWAQPSPNRWDHEESNHELYNSGHLFEAAAAHYRATG
                     KQNFLKIALKNADLLVASFGPDKLRVPPGHQIVETGLIKLYHITNNPQYVELARFFLE
                     LRGDSTSHKLYGEYSQDHLPVTKQTEAVGHAVRAMYMYAGMTDIAAIYQDSAYFEALM
                     KIWENIVTKKMYLTGGVGARHDGEAFGDNYELPNLTAYNETCAAIGSVYWNQRLFRLT
                     GDAKYYDIIERTLYNGLISGISLDGKKFFYPNPLESDGKYQFNQGACTRQPWFDCSCC
                     PTNIIRFLPSVPELIYATIADSVYINLYISSNADIIVNQKKIEIIQQTDYPWDGKINI
                     TVNPEKKTIFTLKLRVPGWARNEVVPGDLYKYIAENPEKVSLLVNGKDENGVLDKGYI
                     EITRKWTRGDKIELTLPLAVRKVVAHEKVTDNVNKVAFECGPIVYCAEEIDNHRISEI
                     TVPLDTKLNITKGTVLTENVNILRGKVDNQELILIPYYVWSNRGVGKMKVWLPINKE"
BASE COUNT         2781 a         2017 c         2267 g         2847 t
ORIGIN      
        1 tgggcgatag tggggaatca tatatctata aataaacgga gtgaatgaaa agcttcggcg
       61 caggcgactc gtgcctgcac gcctcgaaag tgagcactgg attcggcaac ttgaataatc
      121 gaaagattag cgatatttgt gcgattgatt tgagaagtat gagccaacaa agcccttaat
      181 ttgattgcca aatattctgt gccgatatct acgaaaacgt tcggcataaa attttgagtt
      241 gtgggggttt cgtaaaaaag tacgttggga atatagcgcg tggcggaatt ggtgatcgcg
      301 gctatattgc ggtgatcctg atgggtgtca tcgtgataat taaccagaat catatcggga
      361 gtcactttat gaatgatttc ctcgagggcg gctatggaat ttttattgat tgttatatct
      421 gtatcttcaa aactacccca gaaaatgtct ttaacgccca ttatgcgggc tgaattttct
      481 tgttcagccc ggcgcacgtc gggatcgccg cctgcgcctc ccagagtggc tacatacagg
      541 taaactgaat gtcctttggc attgaatttg gctaagctgg caccacatcc gatttcaatg
      601 tcatccggat gagagccgat ggctatgata ttcatctttt tttctgatga gctcatcgaa
      661 ttcccccttg gataatagaa ttggctcgtt gaccttcatt aaataataaa tcgagaattg
      721 acatatttgg taggaaatca ttagtcattt gccgataaac gggatgctcg aacgattgga
      781 acttaagttt gatgtcattc tttgcaaaaa gctcaggttg caggtaatta cagccaccga
      841 ttcctgagag atagcaatca gcccctacgg ctttggtagc aattatcagt cgttcggcgg
      901 cactgatctg ggaattttgg ttgagagctt ttatttccga agcaattact gtcggagtat
      961 caattccgat taaagacatt aataagcgaa ttgcagttat attgaactgg gcgagatttt
     1021 gccactcgaa ttgataaaga ttttcgagct tctcgaagta taacttgaaa aatggtgctc
     1081 gaccatagta agtttgtaga gtttgaagat gttttttgcg ccaattgacc gtgttgttga
     1141 ttttgacctc gtctattttt tgaccaaagt gatgaattac aggaaccgtt agccaaatcc
     1201 agccttgcgg agtgcgaatt ttattgcgat tttgccattc attttttttg aactgaacat
     1261 tgtccagaaa aatgaaaatg tcagcctgca taattttggc aaaataaccc agccacggat
     1321 aagtttgcgg ttgatgaatg gcacagatca ttaaatttcc ttgattttta tcccattttt
     1381 accttagttt gccaatgatg aaaaaattag gagctacaat ggatacaatc attaaaggtt
     1441 gcattatcgt cggagtcatt tccttaatcc taggcatagt ttcacgtctt actttgaagc
     1501 ccttttttgt tgaagcgcag gcatttatgc agttcgcgca gttttgtttc ttagccgcaa
     1561 tcactttttt actttataaa atagcctata aggttaagga ctaactctcc taccgttatt
     1621 tactaacaaa tatcgggttg ctggcgatag ttggccagct gtcgccgcca taaatccggt
     1681 aatagcacat tggattcgac attggcgact catctacgaa ccattcatct aaaacggtat
     1741 tcagatagaa aattttgatc aattcgctat tgcgatagag aaaaattgtt tccggtttat
     1801 tcagcgtagt tagtgctcgc aatcgaatat ggatggcaac cggcgcttgc gcatgagtca
     1861 atgtttctcc gatgaaggca ttattgccgg tgtgcagatc ataaacagaa aaatcatcaa
     1921 tgacgatttt atcgccggtg aaactgcgcg ttgcatacat ccgtccagct cgcatagctt
     1981 cgtaaactcc agcccgcgaa cgctctttga caaataagaa agttaaggtt tcagtgacat
     2041 tgttaaggtt gctggaatcc tcgaaatcga gttcgccgat tgcccagact ggtttgcgac
     2101 gtaagcctgc gcaatattca gccaatgcta tgtcccacaa accgcccggt cggccgatga
     2161 ctttcgcccc ttcccagaaa atggcaaatc cggtataatc ctgggtttcc ataatgcgat
     2221 gaggataagc ttcggtggaa attttaatgg cttgattcat tagggggata tttatctgac
     2281 gaacttcatt atgggtaact tccggatgag cccagaaaat cagaccgtta tgggcattga
     2341 cgtaatcaat tagctcctga taaggagcac tgccagcttt gcggtcgtag ggtgtgtatt
     2401 tacgcggcag gaacggatat tgattatata aaatcagtcc aaatataatc gccataataa
     2461 aagccggaaa tgaaaagcga atattttggc gcggacgatt gatattctta cggctaaata
     2521 atccatattt tcgtcggaaa attaacgcta atgatattag aaataataaa aaatagagtg
     2581 tcatcaacgc aaagtgagta aaattttcgc cgatatactt aataatttta ccatcgggtt
     2641 tgctataacc gatattgtga ttagccagcg aaggtaattg ttcgaaaatt tttggatctt
     2701 cgagaccgaa aacgattaag tgagtatgcc aatctcttag aaccatattc tcataaagcg
     2761 gcgaaccttc ccagcgatag taaggaacag cttcaatgcc cggaattaaa atcaagttag
     2821 gataagccgc gtcgagagcg ttgatttcgt tgatatattt ctccactccg tagcggtgaa
     2881 cggaattttc ttcaatcgta aatttcaaaa aattttcaaa gggtcgaatg ccgtaagaga
     2941 ctttcatatt atcatggtcg gttatgatac cgacatctaa tttgtgacga gcgattatat
     3001 tagccagttc cgccagagaa tacttaccgc cgctgacttg cgaatcgaaa tgcacaccgg
     3061 tctttagtaa aatataacca ggcttagtct gagaaaagac gagagagtca gtcattatta
     3121 gtaaactgac cgttattaaa aaaggtgcga atttcttatt caggcagatt cgtgttacac
     3181 gtttgataag cataaataac tcgcttaata taattatcaa tcgtgaaata ttcagccttt
     3241 tttcggtttg tagaagccat agattgtcgt tgttcaggat tgctggcgat cctctgaatg
     3301 gctgtggcta aagcttcggc atcatccgaa ttaaccagca aaccatttac accatcttga
     3361 attaatgatg ggacgccgcc gacgcgcgtt ccgataatcg gtaagccgca agccatcgcc
     3421 tcgataatca cattgccgaa gccctcgttt agcgaggtaa agacgaagat atccgacgat
     3481 gccataattt ctggaacatc ggtgcggtan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnc aggctttaaa gaatgggtcc aaatcagctc cgccggaagt gacgtagaat
     3661 ttcgaggatg gaccaatgcg ttcgcgaata tgatcctggc gaccaatttc ggttaagttt
     3721 aatactcgat cgcagtaatg tgccgaaaat ttttctaagg ttataaaaca ttttgaaata
     3781 taccggttgt aatagccata aaaaatgtgg ccatgggtcg aatgcaaaat ttttggcact
     3841 ctagctaagc gtccggctaa acgccccaga aacccagcct tggaggtatt ggtgtgtaaa
     3901 atttccggtc ggtattttaa gatcaagcgg taaatctgaa gaaaagccag gggatcataa
     3961 ataggtgaag gatcacgcac taatgttctc agaaaataca gctgaaatcc atttaggcgg
     4021 gcgtattctg ttagatcgaa cggaggatga gtagttctac cagctaccag cagaacttca
     4081 tagccgtttt ttaccagacc ggcgcataac ttcagagtga cattagacga accgccttga
     4141 tcaagacggg ttattaattg tagtactctt ggctgacggg gatttctgtt aatttaacgc
     4201 ctctacgacc gaattaattt tttgccgaat tgcgggaccg atttgagcat gggttggaat
     4261 tagaagtaaa cgttcagcca ggtaagttgc ctgcggataa ttatcataag taaatggatt
     4321 taattccggg aaaaagtggt gcagtggttt ctcgtataaa cgagtggttt ctatgccaac
     4381 cttaagcaat tttttctgta aacgctggcg ctgatccaga tttggaacaa tgaccggaaa
     4441 ttggttgaac gccgcttgcg agttgggcaa aattttcggt atggtgtagc ctggtttccc
     4501 ggtgaaaatc tgcattagcg ctttgccatt ttcgacacgt cttttggtca attgatctat
     4561 acgccgccag agagtttttc ccagagcggc ttgcatatta gtgtaagcaa aggaatcaaa
     4621 atggggatgc agggtcgtat atttgaattt ggcaataaaa ggcaacagca gagtgtaaca
     4681 taatggattt acagcgatgg aaagcccgag aaaattcagc cacattaaga attcggctct
     4741 ttggctaacc ggtggtaatc cggcgactaa ttcttcgata attggtgaat agcgttgatc
     4801 tttccagaca ataatgccac cggctaaagt cgagacgttt ttgccccgat tcagcgaata
     4861 aaatccgaat ggcgcgattg agccggtctg gcgaccacct atggttgaac cgagcgaaga
     4921 agccgcgtct tcgacaataa aaatatcctg tcctgcgatg gattcgttga tcggagctac
     4981 atccatcggc agaccaaaca tatgcaccgg catgatagcc agcgtttgag gagttacggc
     5041 attcaagatt ttgtgcatgt ccatattgaa agtggtcagg tcaacatcaa ctaaaacggc
     5101 attcaggcca gcggcacgaa ttggtaagag taaaacaggc gcagtataag ccggtaagat
     5161 tatttcatta cgttttgggg aagtgcggat cttcttgaat aactccagca atataaaaag
     5221 tgccgtagtg ccggaattga ccaaagctag attttcaaca tcgaaatatt tttttaatgt
     5281 ttgagagaaa ttatcctgga catggtcgct ctcaaacaaa cccagcgtag cacgcgctat
     5341 ttcggtgaaa ttatagggtg ttccggcagt cggtatatgc aaaatactca attcatttct
     5401 cccatttgtt taagtagccc agtggaaaac caggtagcgg tgagggaatc gccatttgtt
     5461 cactattttt aatatttagt cttcgcatta ggggttgaaa ttctttccgg cgatgaccgt
     5521 gaacgttcag gcgtcctgtc agagaactga gccgatatct gaaccataaa tatggattac
     5581 gactgtttcg ataatcgaat actatccagc cgcctttttt acagacacga tgcatttcaa
     5641 taataaatgc ttcaatcttt tcgaaaggat gaaaattaag aaaggtattc aggcaaaaga
     5701 cggcttcaaa tttttcatct ccaaatggta agttaaaacc gtcgccgatt agaagaggtt
     5761 cgatagaaat attattatct ttaaaaagtg ttcgagcttc ataaattgcc tggcggtcgt
     5821 gatcgagtcc aaacgcaatt gccttttttg cgtcagtcac ataatattca agtagaaaca
     5881 gaccgttgcc gcaacccaca tctaaaaaac gtccattgat ttggcgggca attttaagag
     5941 ccgcggttcg cacgcggatg gaagtcaggg tttggaaagc cgcggtacgg gtctgattct
     6001 tgagatttaa agtcgtcata aaattttcat tatttctcgg ctgatgattt gccaatcaaa
     6061 agattggcga gcgcgttcca gtccatattg ccccaattgt tctcttagag atggattttg
     6121 gatgagagat acaagagttt gtcgaatggc ggattgatcg ttggcatcaa ttatgaggcc
     6181 ggagcgcccg ttttcgatag cagacgcgac cccgccgaaa tcaccggcga caaccggttt
     6241 cccgcaagcc gccgcttcaa gaaaaacgat gccgaaccct tcgtaatcgc ctcgtaagcg
     6301 gacgtcgtca taagtctggc gatttaatag gataaaaata tcacatagat tgtaaaattt
     6361 tggtaattcg gcatcaggta tataaccgca aaaaatcacc tggtcagtta cattcaattc
     6421 tttggtcaac tttcggagac gttcttcttc gcgtccggcg ccaacgataa ggtaaatgag
     6481 atctggaaat tgcgatgata agtccgctac agcccgaatc atcacttcgt gacctttgcg
     6541 ttcatcgagc cgggcaactg tcaaaagcac agttttgccg tttagaccgt atttttccat
     6601 taaagtcgca tcttttggag ctggtttgaa aaaaccggtg tccacgcctg gggtaattat
     6661 tttgaatttt tcaggaggaa tgcctaacgc cataaattca ttacaggtaa attggctgtt
     6721 cgggacgata tattctgcgt tggataggaa agtttttatg tatttcatga aaagaggact
     6781 gtacccgaag cggaaagttt cggagccata aacgtaaata ttgtatggga ttccgaataa
     6841 cttgttcatt aaccagccgg taaatccgga cgataatacc tgcccgcaat ggattttacg
     6901 gaaatgatat ttgcgatcca gaaccaatgt ccagaatgcg tatagcaaac cttttagaag
     6961 tttatcgcgg attaagttgc cggctggaat acgtcgccga atagtttgca gtccggtttg
     7021 agagtcgaac tgttcccagc cgggatcttt ggggcaaagg atatagttgt tttcctgcgg
     7081 aagaagtcgc cagatttgat aataataggt tgagatacca ctaactaccg gcggaaaatc
     7141 gccggggatt aaaagggtgt tttgattacg catcttgttc ccccaaggtt atataaccaa
     7201 ttagataacc actcaaataa aataccaaac tcagtaaata aaatgctcgg aaagttacaa
     7261 tagttttaca tagacgccaa acgtaactgc cgatccgaac aattgtagat cgtttctggc
     7321 ttacctcggt tccatgctga taggctaatt cgacgacgaa ttctgggtaa aatttattga
     7381 cgtaagccgc cattttgccg ttgcgataaa attgccgcac gaatttggag aaagtcgacg
     7441 gcggcaaatg gtgaatgtaa agttccggta atactaccac acgataaccg gcttgccgaa
     7501 tttcctggcg cagataagga tcgagtccgc gcggaattaa ttcgttttcg ccgccaattt
     7561 taatgaaaac atcacgcgga ataatgcaac agggatgttc agccaaatcg ctgtcggtaa
     7621 tttcggagac gatcggtgaa gtgcggcggg gcagttcgcg cattactcgc cgtacaaacc
     7681 agttggcatt ttccggaata agattggcga ctccagccag accgatgtcc ggatgatttt
     7741 ccatctgagc cagcattttt gctaataaat cgtcagagcc caggcgcgtg tcatcgtcga
     7801 aaatgaccag gtatttgcct gccgccaaag ccgcaccaca attgattgcc cgtccttgac
     7861 gggtatcgcc gattatcaaa attacctcaa aattgcggaa agtctggcgt tcgatttggc
     7921 tcatcaaccc gggcaaaaat tcctgtcgag cggcacccat tgtagggata atgatgctga
     7981 tcaaaggctc ggtgacattt tccgggcggt gaatttcgat gcgcccttcg gatgcgccct
     8041 tcggatattt aacgaacggg cgggaaatat ttccagtata aggtttactt ataatcacaa
     8101 tatttaataa gttagagata tttgatgaaa atatttgctt ttattcttta ttgattggta
     8161 accatacttt catttttccg actccccggt ttgaccagac ataataggga atcaggatta
     8221 attcttgatt gtcaaccttg cctcttaaaa tattgacatt ttcagttaga actgtgcctt
     8281 ttgtgatatt taatttagta tcgagtggta cggttatctc tgagattcgg tggttatcaa
     8341 tttcttcggc acaatagacg attggaccgc attcgaaagc aactttattg acattgtcag
     8401 ttaccttttc atgagcaacg actttacgga cagccagcgg cagggttagt tctattttat
     8461 caccacgagt ccatttcctg gttatttcga tgtaaccttt atcaagcacg ccattttcgt
     8521 ctttcccgtt tacgagcaaa ctgacttttt caggattttc agcaatgtat ttatataaat
     8581 cacccgggac cacttcgtta cgagcccagc ctggcacacg taattttaag gtgaaaatgg
     8641 tcttcttttc cggattgact gtaatattaa ttttgccatc ccagggataa tcggtctgct
     8701 ggattatctc gatttttttc tggttgacaa ttatatcggc attgctggaa atgtagagat
     8761 taatataaac actgtcagca atcgtggcat aaatcagttc gggtacagaa gggaggaagc
     8821 ggataatatt ggtcgggcaa caggaacagt cgaaccaggg ttgacgcgtg caagctccct
     8881 ggttgaattg atattttcca tccgattcga gtggattggg gtagaagaat tttttcccat
     8941 ccaatgaaat tcctgaaatg agtccgttat acagtgtgcg ttcgataatg tcataatatt
     9001 tcgcatcgcc ggtgagccga aagaggcgct gattccagta aacgctaccg atggcggcac
     9061 aggtctcgtt ataagcagtg agattaggca gttcgtaatt atcaccgaaa gcctcgccat
     9121 cgtgtcttgc gcccacgccg ccggttaaat acattttttt agtgacgata ttttcccaga
     9181 ttttcatcaa agcctcgaaa taagccgaat cttgataaat agccgcaatg tcggtcattc
     9241 cggcatacat atacatagcc cgaacggcat gcccgacggc ttcggtttgt ttggtaaccg
     9301 gcaaatgatc ctgactgtat tctccgtaca atttatgaga agtgctatcg ccgcgcagtt
     9361 ctagaaaaaa tcgagctaac tcgacgtatt ggggattgtt agtaatatga tagagtttga
     9421 ttaacccggt ttcgacaatc tgatgcccgg gcggaacgcg taatttatcc ggtccgaagc
     9481 tggcaaccag cagatcagcg tttttcaagg cgatttttag gaaattttgc ttgccggtag
     9541 cccggtagtg agcggcggcg gcttcgaata aatggccgct gttataaagt tcatgattgg
     9601 attcctcgtg atcccagcga ttcggcgatg gctgagccca ccacgccggt ggatgcatac
     9661 ggtcaatggt aaaccaggta gtgagataac catcgggctc ctgtccgatt ttgattatag
     9721 aaataatgga atccagataa gctgcgagag cgggattagg tcggcttatt aatgaatagg
     9781 atgcgccctc gatgatttta tagacgtcgg tatcgtcaaa gggcatttta cctctgactg
     9841 ggcctttttt gcgaccacca gcaatcagga aattttccat ccgaccttct tcttcgcact
     9901 ttttaaaagc gtattgaatg gtagtgtcct gaatgatttt gatcttcggc aaccagaagg
     9961 cgtcgttaat ttccacgttt ttaatgtcca cagctgatat aggataatcg ga
//
LOCUS       DAOMJU010000079         9989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_6958_length_9989_cov_20.388464, whole genome shotgun sequence.
ACCESSION   DAOMJU010000079 DAOMJU010000000
VERSION     DAOMJU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6958_length_9989_cov_20.388464"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   18..644
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=18..54
                     /rpt_unit_seq="gtctaagtcctttatccactaaaccaaggattgaaac"
     gene            973..>2239
                     /locus_tag="PLO58_07665"
     CDS             973..>2239
                     /locus_tag="PLO58_07665"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQO74703.1"
                     /translation="MKRFLLTIIFLISITAFSLAQISSDWIEQEIRTKTVWLEIQRAA
                     LLQLPSYANTNAYDVTYYALDFRFDIASSILYGEVTIRGLAAESNLQRIDLDMDDSLS
                     VTSIGGDAISYTHRNNILSLQLNKILKENEIFNVSITYSCLEKKNNDRGIYFQYHDST
                     PVISTLSEPYYAHTWFPCKDRLGDKADSVDINITVPSELKAVSNGTLVRIIENDDGTC
                     TYCWQERYPIAVYLISLAISDYTYWSENYFGIDGFSSMPLEFWIYPDFDTIARPFLNM
                     TADMMTYFASLWGEYPFIKEKYGQVQFSWGGGMEHQTATSLGSFSEMLICHELAHSWW
                     GNDVTCASWKHIWLNEGFARYAEALWREKDEGTIGLKNYMTDLNRPSRWRAGSLYVQD
                     TSDVNNIFNQIVYDKGAWVLHMLRGLTGYE"
     assembly_gap    2240..2339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2340..3092
                     /locus_tag="PLO58_07670"
     CDS             <2340..3092
                     /locus_tag="PLO58_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HQO74704.1"
                     /translation="YDKGAWVLHMLRGLTGYEKFFEILKRYRQEYSGGFATTADFQKK
                     CEEIYDQSLDWFFEQWVYGVGQPHYQVNWQAYRHSLNSWKLKVAISQIQTGATIFRMP
                     LTLLFSDSLNDTTFIVTDSLATQYFTFYCNFKPDSVALDPDNWVLKNATYFNSYSEPQ
                     TRPEEFRLHPPYPNPFNQWVNIKLDLPYDIPGRLAIYDLTGREVAILQDGLFKAGQYK
                     TQWQPQGSASGLYFVSFKARYIHLRQKIVYLK"
     gene            3166..3699
                     /locus_tag="PLO58_07675"
     CDS             3166..3699
                     /locus_tag="PLO58_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74705.1"
                     /translation="MLTKIKNALRQPENRRIIAIVILLFIIINVIAWLSLRPYMKQPP
                     CYVCGRAKTRPVKTLYQYRVEVLPYMKSVDIWYCERHYQHAAEIITKLPIKNDSVASR
                     FRIAVIAGTFSLFSLLFIIILLEISFNWLFIHSGLIALTYLIGGVTSNLTVVILLIST
                     FALAIGIFYFWNRWFNK"
     gene            3709..4821
                     /locus_tag="PLO58_07680"
     CDS             3709..4821
                     /locus_tag="PLO58_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQO74706.1"
                     /translation="MDKVDIVIIGAGVVGLAIARELSAKLQAEIVLLEKHPYFGAETS
                     SRNSEVIHGGMYYPTDSLKARFCVRGNRQLYEICQKYRIPYRKTGKLIVASTAEELAK
                     VEALHQQGNLNGVPNLVMLSEKQIREIEPEVRAKNALYSPETGLIDVHRLMQFYESQA
                     LSNGVLIAYQNTVTGIEKQSDGYRITVSDKTGADWDILSQVVINSAGLNADKIAALVG
                     INIDENHYRLHYCKGEYFNVSNRHQGKIQHLIYPVPTDYSLGIHARLRLDGTLSLGPN
                     AIYVDEINYEVDPGHRADFYNSVKDILPYIKYEDLTPEMAGIRAKLQGHGERFRDFVI
                     REESDKGLPGFINLIGIDSPGLTSAPAIAEYVTSLL"
     assembly_gap    4888..4987
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4988..5893)
                     /locus_tag="PLO58_07685"
     CDS             complement(<4988..5893)
                     /locus_tag="PLO58_07685"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQO74707.1"
                     /translation="MGILKKLNSLIGNLLITVALFCLTGELHSEPTSADLSYLKIIKN
                     GLLREGPASYYKIAENLEIGTIVQAFEKHDDWYLIKTHSGRIGWITASDVTPQVQSQD
                     LMAKTNLQNGSPPNIDQKIPFLFTENLELLATGFLWTEPDGSGKIISYLIPGLRVTKL
                     ETNGIFYKIQLQNGLIGWADQSLFTIAEPKLPLSTSKIVAKNGNLRQEPSLSAPVIEK
                     IPKGKSVTIIDSTDGWYKIKTASDLTGWLHQSLLSKQTVSQNGGELSVKILLRNGNLR
                     QSPNLAGKVIQVIPAQTAITVLDSLS"
     gene            6222..7541
                     /locus_tag="PLO58_07690"
     CDS             6222..7541
                     /locus_tag="PLO58_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74708.1"
                     /translation="MEQEKRERIYTFDFAKGVAILLIIPLHALICQIGKNDPAFFQPL
                     ITAIPENVIYLLIPAMILSMWGPIFTLITGANIAYGYLRVYSRNPDDSSAYILRRILS
                     AVMLIFVSRAAVFIFKGGLFTNGFFNIFNYKIRYYADTLDSIALTGIIVPVFILLFLD
                     KSKSIKQKNETGKAVSQKDVKRIYIGMTVITVIWFVFTPVVHALTPYVQDFASKHHLR
                     LLMLVWSKLTAGRFRFFPILGFGYAGAVIGTAIHAKSRYKNIRKYSGIFFLVTLVGFL
                     IWILLKGNPMVNIASDDIPIMMQVLNLGTMTFAMVLVLGYLDYCPPEVRRRRIERTLW
                     IRRFSIVSLTIYVMEFFLAEGVYWVFAQFWDNAVALVNEVPIFIWNFAQIMVFIIVIC
                     LIWGLIVKLWEKVDFIFSLEWFMIKAVALFQRDKHARINSHKILYGQ"
     gene            7544..8635
                     /locus_tag="PLO58_07695"
     CDS             7544..8635
                     /locus_tag="PLO58_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQO74709.1"
                     /translation="MNKVRVGVVGTSWWADGMYLPALQSHPQAEIVAICGRNRPRATE
                     MAEKYNIPKIYTDYRVMIEQGDLDAIIIGVPDDLHYDIAIRSLEAGLNVLCDKPLALK
                     EQQAYEMWQKAEAAHVQNMVLFTYRWMPFFQYVHDLIKQGYIGQCYHAEFSFLVGHGR
                     KPDYQWRLDSKRSNGALGDLGSHLIYLARWLIGEIAQVDAQLASFAKRLGPDGEPISP
                     ANDSAVLLFEFTNGAQGVIHTSFMTYLADRDMQIYVKLYGSDGSLEVNFPSSGSDFTA
                     SIFGANNSDKKFRKLEIPASYWGDVNPAEPFGIFTRQAVGCRLFIDAILENRPVSPNF
                     FDGYKVQQVIEAAINSDRTGKRVAIDHSR"
     gene            8697..9965
                     /locus_tag="PLO58_07700"
     CDS             8697..9965
                     /locus_tag="PLO58_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQO74710.1"
                     /translation="MLEFSPSLVVSDGRGRVFELEQYQALGISGENVVEIPPDEWIPL
                     PEGSQLMELPGRLPVGKDRRSSEVATLTENGAGKLLAVAAFVAPAYTIFYHAAWEKLP
                     RARRLSLFAYSAVGWFDGCFYVPAIRIDPDIRQDPPNFNQAEIARSAQRQLKLFPHNR
                     LVAHLVNNCALTYGCPAALNYLLNRWEMPLPTARTCNSACLGCISLQRNSGVCSAQHR
                     INFTPTVAEIVEIAVPHLDTAPNPVVSFGQGCEGEPLMNPQLLYQAIEGIRQKTARGT
                     INLNSNASRPDVIIKLIEAGLDSLRVSLNSARPQFYTRYFRPRNYTFDAVLESIRLMK
                     NANRFVSINYFTFPGFSDQPSEIKAFEKILADYNIDLIQWRNLNIDPEWYWEELNPPE
                     EDGLGIRQLIEYYQAEFPRLRHGYFNPSLK"
BASE COUNT         2714 a         2030 c         2140 g         2905 t
ORIGIN      
        1 gcctgaacct gtgattggtc taagtccttt atccactaaa ccaaggattg aaacaagttg
       61 aaaaagtaaa caaataaaaa aaaggagagc gtctaagtcc tttatccact aaaccaagga
      121 ttgaaacctg tctcaatctc gtcttcgtcc attgcagcta cgcgtctaag tcctttatcc
      181 actaaaccaa ggattgaaac aaagctggtg gatgcagtat tgttgcattt ggcggttgtc
      241 taagtccttt atccactaaa ccaaggattg aaacaaaaac aggttgccaa gctgaccgcc
      301 cagatcgaaa cgtctaagtc ctttatccac taaaccaagg attgaaaccg acatgttcat
      361 acgacgtcca ttcctttgtc acaaagtcta agtcctttat ccactaaacc aaggattgaa
      421 actttccttt gcgggcagaa attgcctcac gatagatgtc taagtccttt atccactaaa
      481 ccaaggattg aaacctgatg cttttcgttc ttctctatat acgagttttt ttgagtctaa
      541 gtcctttatc cactaaacca aggattgaaa ctcagacacc ccagaaagca aatctgaagc
      601 atgtaaagtc taagtccttt atccactaaa ccaaggattg aaaccaccgg cgaggaaagt
      661 agatattttg gttattcatg aatggcaaag agtgaataaa tatgtggtta attggcaaca
      721 caaaagtctc gctatggact atacatttac cgattaacca ttaaatctgc aaattttaaa
      781 agaatattga acaagaaaat ggtaattata gagtaaagga atatggagtt cctatggagt
      841 gcatatatgg agtgcagtga ccatgtcact gcatgcaccg acacggtcgg tgcactccaa
      901 aattacactc caaagctgca aaattgaaac cgctggaaaa cttatcagca atagttaact
      961 tttcactgcc ttatgaaaag atttttatta actattattt tccttataag catcactgcc
     1021 ttctctctag ctcagattag ctccgattgg atcgagcaag aaatccgtac taaaaccgta
     1081 tggctggaaa tccagcgagc cgcactgctc caactaccga gttacgcaaa tacaaacgct
     1141 tacgacgtaa cttattacgc tctggatttt cggttcgaca ttgccagctc aattttatac
     1201 ggtgaggtta ccatccgcgg attagccgct gaatctaacc ttcaacgaat tgacctcgat
     1261 atggatgaca gcctatcggt aacatcaatt ggcggagatg ccatcagcta tactcatcgc
     1321 aacaatattt tatcattgca actgaataaa attctgaaag agaacgagat cttcaacgtc
     1381 tcgattacct atagttgctt agagaaaaag aataatgaca gagggatata ttttcaatat
     1441 cacgattcaa caccggtcat ttcaactctg agcgaaccct attatgctca tacttggttt
     1501 ccttgcaaag atcggctcgg tgataaagcc gattccgtgg acattaatat taccgtgccg
     1561 tcggaattga aagccgtttc aaacggcact ctggtcagaa taattgaaaa tgatgatggc
     1621 acctgcactt attgctggca ggaacgctac ccgattgccg tttatctaat ttcattagca
     1681 atttccgact acacttattg gagtgaaaat tatttcggta tagacgggtt tagctcaatg
     1741 ccgctggaat tttggattta tccagatttt gacacaattg ctcgtccgtt tctgaatatg
     1801 actgccgata tgatgactta ctttgcttcc ctctggggtg aatatccgtt catcaaagaa
     1861 aaatacggtc aagttcaatt ttcctggggc ggtggtatgg agcaccaaac cgctaccagt
     1921 ctgggatcgt tttctgagat gctgatttgt cacgaattag cgcattcctg gtgggggaat
     1981 gatgttactt gcgccagctg gaagcacatc tggcttaatg aaggctttgc ccgctatgca
     2041 gaggcacttt ggcgggaaaa agatgaaggt accattggat taaagaatta catgacggat
     2101 ctgaatcgcc cttctcgctg gcgcgccggt agtttgtatg ttcaggatac cagtgacgtt
     2161 aacaatatct tcaatcagat agtatacgat aaaggcgcct gggtattgca tatgctaaga
     2221 ggcttgaccg gatatgaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2341 acgataaagg cgcctgggta ttgcatatgc taagaggctt gaccggatat gaaaaatttt
     2401 tcgaaattct taagcgatac cgccaggaat actccggcgg ttttgccacc actgccgatt
     2461 tccagaaaaa gtgtgaagaa atttacgacc aaagtttaga ttggttcttc gagcaatggg
     2521 tttacggcgt cggtcagcct cattaccagg ttaattggca agcctatcgc cattctctta
     2581 attcctggaa attaaaggtt gctatctcac aaatccagac cggtgcaaca attttccgaa
     2641 tgccgctgac tttgttgttt agcgacagcc tcaatgatac aacttttata gtcaccgaca
     2701 gtttggcaac ccaatatttc actttttact gcaattttaa accggattcc gtcgccctcg
     2761 atccagacaa ttgggtatta aagaatgcga cttatttcaa ttcctactcg gagccgcaga
     2821 ctcgaccgga ggagttccgg cttcatccgc cctaccccaa tccttttaac caatgggtaa
     2881 atataaaatt agatttgccg tatgacatcc ccgggcggct agcaatttac gatttaaccg
     2941 gccgggaagt cgccattcta caagatggtt tattcaaagc cggtcaatat aaaacccaat
     3001 ggcaaccgca aggtagcgcc tccggtcttt atttcgtcag tttcaaagcg aggtacattc
     3061 atctccggca aaaaattgta tatctgaagt aaaatttatc ttaaccgcca gaattaattt
     3121 ttataaattc agcccaaaat ataaacaacg agaattttga gataaatgct gactaaaatt
     3181 aaaaatgctt tgcgccaacc ggagaaccgg cgaattattg ccattgttat cttgctattt
     3241 attatcataa atgtgatagc ctggctatcg cttcgcccgt atatgaaaca gccgccttgt
     3301 tatgtttgcg gacgggcaaa gacccgtccg gtcaaaacgc tttatcaata ccgagttgag
     3361 gttttgccct atatgaaatc cgttgacatt tggtactgcg aacggcatta tcaacatgcg
     3421 gctgagatta tcaccaagtt accgattaag aatgattcgg tggctagtcg ttttagaatc
     3481 gccgttatcg ccggaacttt ttcacttttt tcgctactat ttataatcat tttactggaa
     3541 attagtttta actggctttt tatccattct ggattaattg cgcttactta cttgatagga
     3601 ggcgtgactt ccaacttaac tgtggtaata ttattaattt caacctttgc actcgcaatc
     3661 ggaatttttt atttttggaa tcgttggttc aataaataat agactaaaat ggacaaggtg
     3721 gatatcgtaa ttatcggagc tggtgtggtt ggattagcaa tcgcacgtga actttccgca
     3781 aaacttcagg cagaaattgt gttgctggaa aaacaccctt atttcggagc agaaacctct
     3841 tcgcgtaaca gcgaggtcat tcatggcgga atgtattatc cgacggacag tctcaaagcc
     3901 cggttttgcg tgcgcggtaa tcgtcaactc tacgaaattt gtcaaaaata ccgaatcccc
     3961 tatcgcaaaa ccggcaaact gattgttgct tctaccgctg aagaattggc aaaagtcgag
     4021 gcactccacc agcaaggcaa tctgaatggt gtacccaatc tggtgatgtt gagcgaaaaa
     4081 cagattcggg aaatagaacc ggaagtgagg gcgaaaaatg ctctatacag tccagaaacc
     4141 ggcttaattg atgttcaccg tctaatgcag ttttatgagt cgcaagccct ttcaaacggc
     4201 gtcttgatcg cctaccaaaa tactgtaacc gggatagaga aacagtccga tgggtacagg
     4261 attacagtat ccgataaaac aggtgcagac tgggatattt taagtcaagt tgtgattaat
     4321 tcggctgggc tgaatgcaga caaaatcgcg gctttagttg gtatcaatat tgacgagaat
     4381 cattatcgtt tgcattattg caaaggtgaa tatttcaatg tttctaaccg gcatcagggg
     4441 aaaatccagc acctaattta tccagtaccg actgattata gcctgggcat tcatgctcgc
     4501 ctacgattag acgggacgct gtcactcgga ccaaatgcta tttacgttga tgaaattaat
     4561 tacgaggtag atcccgggca tagagcagat ttttataaca gcgtgaaaga tattttacct
     4621 tatattaaat acgaagacct tacgcccgaa atggctggga ttcgagcaaa gttgcaggga
     4681 catggtgaaa ggtttcgcga ttttgtgatt cgtgaggaaa gtgataaagg attgccgggt
     4741 tttatcaatt taattggcat tgattcaccg ggattgactt cggcaccagc catcgccgaa
     4801 tatgtaacca gcttgcttta aattaataag ccattttttc aattaaaact tgtaggacag
     4861 cctttcaagg ctgtcgaaaa tagacaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnaga aaggctatcc agaaccgtta tcgcagtttg agccggtatt acttggatga
     5041 ctttgccagc caggtttggt gactgacgta aatttccgtt tcgtagcaga atctttacgg
     5101 atagttcacc gccgttttgc gatacggttt gcttacttag tagactctga tgaagccagc
     5161 cggttaaatc gctcgcggtt ttgattttgt accaaccatc ggtgctatct atgatcgtaa
     5221 cagatttacc tttaggaatc ttctcaataa ccggagcgga caggctcggt tcttgtctta
     5281 gattgccatt tttagcgacg attttagaag tcgatagcgg taatttcggt tccgcgatag
     5341 taaaaaggct ttggtctgcc caaccaatta agccattctg taattgtatt ttataaaaaa
     5401 taccattggt ttcgagcttt gttacccgaa gtccaggaat taaatagctg attattttcc
     5461 ccgaaccgtc cggttcggtc caaagaaagc cagttgcgag aagttcaagg ttttctgtaa
     5521 acaggaatgg tatcttctgg tcaatattcg gcgggctacc attttgcaag ttagtttttg
     5581 ccattaagtc ttgggattga acttggggtg ttacatcgct ggctgtaatc cagccgattc
     5641 gtccggagtg tgttttaatt agataccaat cgtcgtgttt ctcaaaagct tgaacaatcg
     5701 taccgatttc tagattttcg gcaattttat agtaggatgc cgggccttcg cgtaacaagc
     5761 cgtttttgat tatttttaaa taagaaaggt cagccgaggt gggttccgaa tggagctcgc
     5821 ccgttagaca aaataaggct accgttatta acagattacc aataagacta tttaattttt
     5881 ttaaaatgcc cattctcaaa atcgcgtggc aatttacaaa atcaagtgat ttctgtaaag
     5941 tgacttgcaa tttttttatt gctcccaatt ggataaaata gtaattattt gtagggaatc
     6001 atctttatgt catttgatag ttgttaagac tttcctacat caaaaataac ttgagcgaaa
     6061 tagacaaggc gattcattca gctgatttac tctccagccg attatgttcc caaaagtaaa
     6121 ttgacccagc tatgaggttt aatttgctag tgattttctt ggttcatgtc tggctcggta
     6181 gtatattata aaaaatatgt agtgattgca ttttattaaa tatggaacag gaaaaacgcg
     6241 aaagaatata caccttcgat tttgctaagg gagtagccat tctcctgatt attccattgc
     6301 atgccttgat atgtcaaatc ggtaagaacg acccggcttt tttccagcct ttaataacgg
     6361 ccatccctga gaatgtgatt tacctactga taccagctat gatattatca atgtggggcc
     6421 cgatttttac tctgataact ggcgctaata tcgcctacgg ctatttacgt gtatatagtc
     6481 gtaatccgga tgatagttca gcctatattc tgcggcgaat tctttcagcc gtcatgctga
     6541 tttttgttag tcgagcggct gtgttcattt tcaaaggtgg tttgtttacg aatggttttt
     6601 tcaatatttt caattacaaa attcgctatt atgccgatac gctggattcg attgcgctga
     6661 ccggaattat cgtaccggta tttattttac tttttctgga taagtccaag tcaataaagc
     6721 aaaaaaatga aaccggtaaa gccgttagcc aaaaggacgt caaacgtatt tatatcggta
     6781 tgacggtaat tactgtgatt tggtttgtat ttactccggt agtgcatgct ctgacgccct
     6841 atgtgcagga ttttgcctcc aaacatcatt tgcgattatt gatgttggtc tggagtaaat
     6901 tgacagcggg gcggtttcgc ttcttcccaa tcctgggttt tggatatgcc ggtgcggtta
     6961 tcgggacggc tattcatgcc aaaagtcgct ataaaaatat tcgtaaatat tccggtatat
     7021 tctttttagt aacgctggtg ggatttttga tctggatttt gcttaaggga aatccaatgg
     7081 tcaatatagc ttcggatgac attccgatta tgatgcaagt attaaatctg ggaacaatga
     7141 catttgcgat ggttctggtg ctgggttatt tagattattg tccaccggaa gttcgccgac
     7201 gtcggatcga acggactctg tggattcggc ggtttagtat tgtgtcattg acgatttacg
     7261 ttatggaatt ttttctggcg gagggtgttt actgggtttt tgcccaattc tgggataatg
     7321 ccgtagccct tgtgaatgaa gtcccaatat ttatctggaa tttcgcccaa attatggtat
     7381 ttataattgt catttgtcta atctggggtt tgatcgtcaa actctgggag aaagtggatt
     7441 ttattttttc actggaatgg tttatgatta aagcggttgc tctttttcag cgtgacaaac
     7501 atgcccggat taattcccat aaaattctat atggtcaata actatgaata aagtcagagt
     7561 cggcgtcgtc ggaaccagct ggtgggcgga cggaatgtac ctaccggctt tgcaaagcca
     7621 tccgcaggca gagattgttg caatctgtgg ccggaatcgc ccgcgtgcca ccgaaatggc
     7681 tgagaaatat aacattccaa aaatatatac tgattaccgg gtaatgatcg aacagggtga
     7741 tttggatgca ataatcatcg gcgttcctga cgatttgcac tatgatattg caatacgaag
     7801 tctcgaagcc ggtttgaacg tgctttgtga taaaccgctg gcgttaaagg agcaacaggc
     7861 ttacgaaatg tggcaaaaag cggaagcggc tcacgtccaa aatatggttt tatttactta
     7921 ccgctggatg ccgttttttc aatatgtgca tgatttaatt aaacaaggat atattggaca
     7981 atgttaccat gccgagttca gctttttagt tggtcacggc cgtaagccgg attatcaatg
     8041 gcgactggat agcaagcggt ctaatggcgc tctcggcgat ctgggatcac atttgatcta
     8101 tttggcgcgc tggctgattg gtgaaattgc ccaggttgat gcgcaattgg cttccttcgc
     8161 taaacggcta ggaccagatg gcgagccaat atctccagct aatgattcgg ctgttttatt
     8221 gtttgaattt accaacgggg cgcaaggagt aattcacaca agttttatga cttatctggc
     8281 tgatcgcgat atgcaaattt acgtcaagtt atatggttcc gatggtagtc tggaagttaa
     8341 tttcccgagt tcgggcagcg attttacggc gagcattttc ggcgccaata actctgacaa
     8401 aaaattccgg aaattagaga taccagcctc gtattggggt gatgtgaatc cggcggaacc
     8461 ttttggaatt tttacccggc aagcggtggg gtgtcgtctg ttcattgatg ccatattgga
     8521 aaatcggccg gtcagtccca attttttcga tggatataaa gtccaacagg ttatcgaagc
     8581 cgcgattaat tctgaccgta cgggaaaaag agtagccatt gatcattcgc ggtaaattgc
     8641 ctgttaacgg acgtaacttt tcgcttaaag aaatcaatta atgatggtgc aaacaattgc
     8701 ttgaattttc tccttcactg gtggtgtccg atggtcgagg tcgcgttttc gaacttgagc
     8761 aatatcaagc gctgggcatt tccggtgaaa acgtcgtaga aatacctccc gatgaatgga
     8821 ttccgctccc ggaaggtagt caactaatgg aattacccgg tcgtctgccg gttggcaaag
     8881 accgacggag tagtgaagta gcgactctga ccgaaaacgg cgctggaaag ttgctggcgg
     8941 tcgcggcttt tgtggcgccg gcttacacta ttttttatca cgccgcttgg gaaaaattgc
     9001 cacgtgcccg gcgactttca ctttttgctt actcagctgt tggctggttt gatggatgct
     9061 tttacgttcc ggcaatacgt attgacccgg acatccgtca ggatccgccg aacttcaatc
     9121 aggcagaaat tgccagatca gcccaacgtc agttaaaact ttttccccat aaccggttag
     9181 tggcgcatct cgtcaataat tgcgctttaa cttacggctg tccagcggct ttgaattact
     9241 tattgaatcg ctgggaaatg ccgctaccaa cggcgcgcac ctgcaactcc gcttgcttgg
     9301 gttgtatttc tctccaacga aattctggcg tttgcagtgc tcagcatcgc attaatttca
     9361 ctccaaccgt ggcagaaatc gttgaaattg ccgtgccaca tcttgacacc gcgcccaatc
     9421 cggtggtcag ctttggacag ggttgtgagg gcgagccgct gatgaatccg caattactct
     9481 atcaagctat cgaaggcatt cggcaaaaaa ccgccagagg cactatcaat ctaaatagca
     9541 atgccagtcg tcccgatgta attataaaat taattgaagc tggtttagac tccttgcggg
     9601 tctcattgaa ttcggctcga ccgcaatttt acactcgcta ctttcgtccc aggaattata
     9661 catttgatgc cgtactggaa tcaatccgtc taatgaaaaa cgctaatcgt tttgtctcta
     9721 tcaattattt tacttttcct ggttttagtg accagccttc ggaaatcaaa gcctttgaaa
     9781 aaatactagc tgattataat atagatctga ttcagtggcg caacttgaac attgacccgg
     9841 aatggtactg ggaggaactt aatccgccgg aagaagacgg cctcggtatt cggcaattaa
     9901 tcgaatacta tcaagctgaa tttccccgcc tacgtcacgg ttatttcaat ccctctttga
     9961 agtaatcaaa gctattccac caccaaggc
//
LOCUS       DAOMJU010000080         9867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_7096_length_9867_cov_20.543620, whole genome shotgun sequence.
ACCESSION   DAOMJU010000080 DAOMJU010000000
VERSION     DAOMJU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7096_length_9867_cov_20.543620"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1558
                     /locus_tag="PLO58_07705"
     CDS             278..1558
                     /locus_tag="PLO58_07705"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HQO74711.1"
                     /translation="MHAYSFQLYSLALLATIIIATVTAIVLWPRRKFKGVLWMILLEI
                     AAAEWAFGILFESAATTHRLKELWSAIAYIGTCSVPLFFFLFTVEYYRGRLQISKIAL
                     TFLITIPIFIIILAFTNPLHNLLWPNITINPATNIAIYSHGIVWWITYVYEYALIIIG
                     MYFLLKMAFESGSFYTPHNLTIIIGALIPIIGNLIYVFGPNPVPGLDWAPVGFALSGI
                     VITWAILRLQLFKLRPIAYSFLVDKMLDGMIILDTNNVIVDINPASANIFHKRPKQMI
                     GQKINRFLPQSIDLKNLLLAENHSQIEIALGKIQPDRIFELRLSILRDNYSQIIGKSL
                     LLRDITEKKKNENEREKLIRDLQNAMSQIKTLSGLLPICAHCKKIRDDKGYWHAVEEY
                     IHEHSDADFSHSICPDCLEKYYPEIAKKMKSSQS"
     assembly_gap    1811..1910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1911..2137
                     /locus_tag="PLO58_07710"
     CDS             <1911..2137
                     /locus_tag="PLO58_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQO74712.1"
                     /translation="GNVTFFAFDEGQRLSEHTAPFDALVQILDGEAEITIAGQPFSVK
                     ADEMIIMPANQPHAVKAIQRFKMLLTMIKG"
     gene            complement(2262..3452)
                     /locus_tag="PLO58_07715"
     CDS             complement(2262..3452)
                     /locus_tag="PLO58_07715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQO74713.1"
                     /translation="MSVKVLIKKDREKSLLRHHPWLFSGAIERIEGEPQLGETVELRA
                     HDGRFLGFGAWSPKSQIAVRIWSFNPDDEINADFLRRQLSWAIKRRESLFEPGKTDAV
                     RLVNAESDGLPGLIVDRYNGYLVVQFLSAGAEFWRETIVTVLAEIVPVTGIYERSDVE
                     VREKEGLELRRGLLQGNEPSELIEICENSRRFLVDIRNGHKTGFYLDQRDNRQKITEF
                     SARREVLNCFAYTGGFALAALSAGATKVTNVETSAEALELLNRNVALNGFNPAKVENL
                     TDDVFQVLRKFRDQGRSFDLIILDPPKFAESKSQIERASRGYKDINLLAFKLLRPGGI
                     LFTFSCSGQISPELFQKIVADSALDAGKSAQILVWLNQSADHPTALNFPEGHYLKGLV
                     CVVD"
     gene            complement(3462..>5255)
                     /gene="selB"
                     /locus_tag="PLO58_07720"
     CDS             complement(3462..>5255)
                     /gene="selB"
                     /locus_tag="PLO58_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HQO74714.1"
                     /translation="LAEEHLRGMTIDIGFAFLNENIAFIDVPGHERFIKNMVTGASTI
                     DVAMLVIAADDGIMPQTREHFEILKLLGIRHGIVVVNKIDLVQPDWLEMVVEDIRQLV
                     EGSFLENAPLFKTSTVTGSGIAELRRFLIELPNQIPEVRSTGLFRLPVDRVFSIKGFG
                     TVVTGTVLSGEIKVGEEIEIMPSGKRAKIRSLQSHNQPLDSVARGQRAALNLVGVSKE
                     QIERGMIIATPGAFVKTNLLTVYIESLPSAPPIEYNDQLRLHLGTGEYIVRIRLIGRD
                     RLFPGKQGVAQIVLDQPISAGFRDHFILRSYSPLVTIGGGIVLENSPEPLRKKNKETA
                     AELERLVCAGNEEWLKWLIFRQGEKLITIEKLAQVLTATVAEISPLLAKLVKENVIRE
                     REGKIIAAERLAECAEKITATLAEFHRQNPLLPGLEKATLFGNLRFDETLGDWLTEDL
                     TQAGKIRVIGDKLAIAGFAPQLSERQTTRQEQILAEITKSALTPPTLAELAEKLQTDL
                     AEMRQLINLLVSQQEVVLIDRIYPFSAAAVAEAETKLIEFLRGKSGATVSELKEVLGI
                     SRKFALPLLNYFDQKGMTARSGDKRRLGSID"
     assembly_gap    5256..5355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5512..6930)
                     /gene="selA"
                     /locus_tag="PLO58_07725"
     CDS             complement(5512..6930)
                     /gene="selA"
                     /locus_tag="PLO58_07725"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HQO74715.1"
                     /translation="MAGRENYPNELKRLPSVQKVLDRLAQSESPIKPAILTTLVRQEL
                     NELRAAIRTGNFISPKSDDLIEVVAEKVAGRINALTNCCLKKVINATGIVLHTGLGRA
                     PFGTEILPKMAELLSGYVNLEFDLDSGERGERLNLTNEKICLLTGAESSAVVNNNAAA
                     VWLALNSLADSREVIVSRGELIEIGGSFRLPDIMAKSGARLVEVGTTNRTYLQDYQKA
                     RTRKTGAILRAHSSNYRIEGFTTKPTASEIITWAHSQNLPVIFDLGSGALFPMENVEL
                     PYEPVVAEVVKQGFDLITFSGDKLLGGPQAGIIVGREKYIRCLRKNPLMRILRCDKTT
                     FFLLDYTLSQYLLDKPFPEIETYRLLTTKPAQLKAQAIQILNAIEPEIIKTLNLTVRE
                     VPTEAGSGAMPTVQIPSVAIVAIPRYISETQLAQKFRSYSPPIVGYCRNGEFRLDLKA
                     VQPNDLPLIQSALQAIGRSLIN"
     gene            complement(6942..8786)
                     /locus_tag="PLO58_07730"
     CDS             complement(6942..8786)
                     /locus_tag="PLO58_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQO74716.1"
                     /translation="MNIYRRVLKLVKPYWAVLTVSIITSLIYVALNSTSIWLTASFTK
                     VLFPATSDTETSQVIPVVPEKGKGSLNDTLKNYTDRLILRDTPLESLRALCLIIFLTF
                     LLKNVFLYIKNVSVGFVQLRMINDVRNKLYERLHDLSLSYFNRKRSGEITSIIMNDVN
                     AMRNSFTVSFDKLLVEPINILVFMVLLFIISWQLALLAVVILPVSGFIISKIGKSIRR
                     KSLRSSRQIAGIMAILDETLQGMRVVKAFAMEKFEIDRFRKESRKYFNLVFRRKKLRE
                     FSSPINEVFGVFIGVLLLWFGGSLVLTGKGIDAEDFVRFAVMLFAIMNPIKSLTNVSM
                     EMQEGLASAQRVFSLLDEKPDVVEKPNAIEIKGFNDKIVYDNVSFKYETSRTEVLSGV
                     NLEIKKGEIVAIVGHSGAGKSTLVDLLPRFYDPTGGRILIDGYDIRDLTFASLRSLMG
                     IVTQHTILFNDTIFNNIAYGMQDFDPEKVRAAAEAANALEFIEEFPEKFETMIGDKGL
                     RLSGGQRQRLAIARALLKNPPILILDEATSSLDSESEQKVQAAIENLMKNRTVLVIAH
                     RLSTIHNADKIVVLEHGRIVEIGTHNGLLSREDSIYRTFYETQFAKNE"
     gene            complement(8783..9418)
                     /locus_tag="PLO58_07735"
     CDS             complement(8783..9418)
                     /locus_tag="PLO58_07735"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQO74717.1"
                     /translation="MPEIISLARAADIDCQKVLATLQSGAIIAYPTDTIYGLGVDAFQ
                     PQAVQKLLQLKGRTTGKPISVLYADVPSLLSDFKHLNDYQRRIVKTLLPGKITLILPL
                     SSNSIFPVEISADGSVGVRVISLEPMNRILRQYPHPITTTSVNPANAPPAHSAAEIIS
                     YFGDQISLILKYEIADDSLPSTLVKVGKSELEIIRIGAVTMAEIEEKMKQL"
     gene            complement(9411..>9867)
                     /locus_tag="PLO58_07740"
     CDS             complement(9411..>9867)
                     /locus_tag="PLO58_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQO74718.1"
                     /translation="MIFDARSQSGRNKEQIFYKSKANSPRIWKYLRDENILYLVADQD
                     ARRDGIFVKFFDQWSSSHRGPALFALRQKAPLIAASCLMNTKGKYVIRLKELNTDVPP
                     ENKSDPVAWLTQQIAIYFEEQIRKCPEQYYWFHRRWKTKPPASFIEQNA"
BASE COUNT         2702 a         2214 c         2089 g         2662 t
ORIGIN      
        1 caggcggttt ttcgaaaaca aataatcaag attgatgacc agacaaatag agttcttcag
       61 atatgaaata ttccaggata aattttggaa tagtggcatc tgccaatatc gggagaaaaa
      121 acgctacttg cctcactgaa aacatcttta cattctgcat ctaaatgtaa atgttagctc
      181 ttgattatac gcaaattaat gaacttagat ctcaattccc tttgtaatat gggatagatt
      241 atttaggttt atctgatatt tttttggagc tattaatatg catgcttatt cctttcaatt
      301 atatagtctt gccttgttgg caaccattat cattgcgaca gttactgcta tagtactctg
      361 gccgcgtcgc aaattcaaag gtgtactatg gatgatctta cttgaaatcg ccgcagcgga
      421 gtgggcattc ggtattttat tcgaatcagc cgccactact caccgattga aagaattatg
      481 gtcggcaatt gcctatattg gtacttgttc tgttccactc ttcttttttc tcttcacggt
      541 tgaatattac cgaggacgat tacagatttc taaaatcgca ctaacgtttc tcataaccat
      601 tccaattttc atcattattt tggcattcac taatcctttg cacaatttgc tctggcccaa
      661 tattaccatc aacccggcaa ctaatatagc gatctattct catggaatcg tgtggtggat
      721 tacttacgtt tatgaatacg ctcttatcat tattggaatg tatttcctac taaagatggc
      781 gtttgaatcc ggctctttct atacgcctca taatctgacg ataattatag gtgctttaat
      841 accaataatc ggcaacctga tatatgtttt cggtccgaat cctgtgccag gattagattg
      901 ggctccagtt ggattcgctt tatcgggaat tgtaatcacc tgggcaattc tgagattaca
      961 attatttaaa cttagaccaa tcgcttattc atttttagta gataaaatgc tcgatggaat
     1021 gattattctg gacactaata atgtaatcgt agatataaat ccagcgtctg ccaatatctt
     1081 tcataaacga cccaagcaaa tgattggcca aaaaataaat cgttttctcc ctcaatcaat
     1141 tgatctaaag aatttgttac ttgccgaaaa tcatagccag atcgaaatag ccctgggtaa
     1201 aatccaaccg gatcgcatat ttgaactacg gctctctatt ttgcgagata actattccca
     1261 gataattggt aaaagtctcc ttttacgcga catcaccgaa aaaaagaaaa acgaaaatga
     1321 gcgtgaaaaa ttaatccgtg acctacaaaa tgctatgagc caaattaaaa ctctcagcgg
     1381 tcttttgcca atctgtgcgc attgtaaaaa aatacgcgac gataaaggtt attggcacgc
     1441 cgttgaggaa tacattcatg aacattccga tgctgatttt tcccatagta tttgtccgga
     1501 ttgtctcgaa aaatattatc ctgaaattgc gaaaaaaatg aaatcaagcc agagttaaat
     1561 atccagctaa cgtctcatta aattacctta aaaattaatt atcaatactg ctaataattg
     1621 aaaggataac gaaaatgaaa gccgaaggga aaaattcaga acaagatcaa tatcggtctc
     1681 gaatattaaa aacggccgat ctgggtaatt atcagaccgg agcaatcgtt agccggacct
     1741 tgattgacag accggctgga aacgtgactt tcttcgcctt cgacgaaggt cagcgactga
     1801 gcgaacacac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctggaaacgt
     1921 gactttcttc gccttcgacg aaggtcagcg actgagcgaa cacaccgcgc ctttcgatgc
     1981 actggtgcaa attctcgacg gcgaagccga aatcacgatt gccggtcaac cgttcagcgt
     2041 caaagccgac gaaatgatta ttatgcccgc caatcagccg cacgccgtga aagccatcca
     2101 acgcttcaaa atgttgttga cgatgattaa aggataaaac tcaccgtcta tagtaatcga
     2161 ataagtctcg attattatcc ggatcagggt aaaaatcatt tctctcgcaa acttgcggtg
     2221 ctactcttta tcttaatatt gcgactggag tttcacgtct atcaatcaac aacgcagact
     2281 aatcctttga gataatgacc ttcggggaag tttaaagccg tcgggtgatc ggccgactga
     2341 ttgagccaga ccaagatttg ggctgatttt ccggcatcga gggcagaatc agctacgatt
     2401 ttctgaaata attccggcga tatctggcct gaacaagaga aggtaaaaag tataccgccc
     2461 ggccgtaaaa gcttaaacgc cagcaggttg atgtccttat agccgcgact ggcacgttca
     2521 atttgacttt tcgactcggc aaatttggga ggatcgagga taattaaatc gaatgaccgt
     2581 ccttgatcac gaaactttcg cagaacttga aaaacatcat cagtcaggtt ctcgacctta
     2641 gccggattga aaccattcag ggcaacattg cggttcagta attcgagagc ttcggcggaa
     2701 gtttcgacat tcgttacttt ggtggcaccg gctgacaaag ccgcgagcgc aaatccaccg
     2761 gtataagcaa aacaattcag cacttcgcgc ctagctgaga actcggtgat cttttgtcgg
     2821 ttgtcgcgct ggtcgaggta aaaaccagtc ttgtgaccat tgcgaatatc aactaaaaaa
     2881 cgccgagagt tttcgcagat ttcgattaat tccgatggtt cgtttccttg taataaaccc
     2941 cgacgaagtt ccaaaccttc tttttcgcgc acttcaacat ccgaacgctc ataaattccg
     3001 gtaaccggga cgatttcggc cagcaccgtg acgatcgtct cacgccagaa ttcagctccg
     3061 gctgaaagga attgaactac caaataaccg ttataacgat ccacgatcag tcctggtaaa
     3121 ccgtcggatt cggcgttgac taagcggact gcatcagttt tgcccggctc aaaaagcgac
     3181 tcgcggcgtt tgatcgccca actcaattga cgtcgcaaaa agtcagcgtt gatttcatca
     3241 tccggattga aactccagat gcgcacggca atttgagact ttggcgacca ggcgccgaat
     3301 cctagaaaac gaccgtcatg tgcccgtaac tcgacggttt cacccaattg cggttcgcct
     3361 tcaattcttt caatcgcacc tgaaaagagc cacggatggt gacgcaggag cgatttttca
     3421 cgatctttct ttattaatac ctttacactc atactatttc cttagtcaat agaccccagc
     3481 ctgcgtttat ctccgctccg cgccgtcatc cctttttggt cgaagtaatt caacagtggc
     3541 aaggcaaatt tgcgcgatat accgagcact tccttcaact cgctgacggt agcgccgctt
     3601 tttccacgta ggaattctat caactttgtt tccgcctcgg cgactgccgc cgcgctgaac
     3661 ggatatattc tgtcaattaa aactacttct tgttgactga ctaagaggtt tatcaattga
     3721 cgcatttcgg cgagatcggt ctgtaatttc tcagccagtt cggctaaagt aggcggcgtc
     3781 aaagctgatt ttgtgatttc agccagaatt tgttcctgcc gagtagtttg ccgctcgctt
     3841 aattgcggag caaaaccggc aatagctaat ttatcgccga tgaccctgat tttgcccgcc
     3901 tgagtcaaat cctcagtcaa ccaatcgccc agtgtttcat cgaaacgcaa attgccgaat
     3961 aacgtggctt tttccagacc gggaaggagg ggattctgtc ggtgaaattc agctaaagtt
     4021 gcggtaattt tttcagcgca ttcagccaat cgttcggcgg cgataatttt gccctcgcgc
     4081 tctcgaatga cattttcctt aaccaatttc gccaatagcg gtgaaatttc agccacagta
     4141 gcggtcagaa cttgcgccag tttttcgatt gtaatcagtt tttcaccctg gcggaatatt
     4201 aaccacttca accattcctc atttccggcg caaaccaacc gttcaagttc tgcggcagtc
     4261 tctttgttct tcttgcgcaa tggttccgga gaattttcca gcacaattcc accgccaatc
     4321 gttaccaaag gtgaatacga gcggagaata aaatgatcac gaaatccagc gctaatcggc
     4381 tgatcgagaa ctatttgcgc gactccttgc tttcccggga agaggcgatc gcgaccaatc
     4441 aatctgatcc gcacaatata ctcaccggtg ccgagatgca atcgcaactg atcattgtat
     4501 tcaatcggcg gcgccgacgg cagagattct atataaaccg tcagcaaatt cgttttcacg
     4561 aaagcgccgg gcgttgcgat aatcattccg cgttcgattt gttctttact tactccaacc
     4621 agattaaggg ccgcacgttg accgcgggca acggaatcaa gaggttgatt atgcgattgc
     4681 agactgcgaa ttttcgcgcg ctttccggac ggcataatct caatttcttc gccaacttta
     4741 atttcaccag agagcaccgt ccccgtgaca actgtgccga agcctttgat cgaaaaaacg
     4801 cgatctaccg gcaggcgaaa caaaccggtg gaacgcactt cgggaatctg gttgggcaat
     4861 tcaatcaaaa accggcgcaa ttccgcaatt ccgctaccgg taactgtcga agttttaaaa
     4921 agcggcgcat tttccagaaa agaaccttcg actaattgtc gaatatcctc tacgaccatt
     4981 tccagccaat cgggctgaac gaggtcaatt ttgttcacga cgacgatccc gtgccgaatt
     5041 cccagcagtt tgagaatttc aaaatgctca cgggtttgcg gcattatgcc gtcatcggcg
     5101 gcaattacaa gcatagcgac atcaatagta ctggcaccag tcaccatatt tttgataaaa
     5161 cgttcatgcc ccggcacatc aataaacgca atattttcat ttaaaaaggc gaagccgatg
     5221 tcaatggtca taccgcgcag gtgttcttct gccagnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnttaaa aaggcgaagc cgatgtcaat ggtcataccg cgcaggtgtt
     5401 cttctgccag acgatcaggg tcggtaccgg taagggcttt gactagcgcc gatttcccat
     5461 gatcaatatg tccggcggta ccgataatga gagattgcat tttctttata attaatttat
     5521 taaggaacga ccgatcgcct gtaaggcaga ttgaataagc ggcaaatcat taggttgaac
     5581 ggctttcaaa tccaggcgaa attctccgtt ccggcagtag ccgacaatcg gcggtgagta
     5641 actgcgaaat ttttgggcta attgggtctc ggaaatataa cggggaattg ccacgatggc
     5701 aacgctggga atttggacag ttggcatagc accactccca gcctcagtcg gtacttcacg
     5761 caccgttagg ttgagcgttt taataatttc cggctcaata gcatttagga tctggatagc
     5821 ttgggctttt agctgagcgg gcttagttgt tagcaaacga taagtctcaa tttccggaaa
     5881 cggtttgtca agcaaatatt ggctaagagt ataatcgagc agaaaaaatg ttgttttatc
     5941 acaacgtaag atgcgcatca gcggattttt acgtaagcac ctgatatatt tttcgcgacc
     6001 gacgattatc cccgcttgtg gtccacccag caatttatca ccgctaaaag taatcaaatc
     6061 gaatccttgt ttgactacct cggctacgac cggctcgtag ggcaattcta cattttccat
     6121 aggaaaaagc gcaccgctac caaggtcgaa aatgaccggc aggttttgag aatgcgccca
     6181 ggtaatgatt tcggatgcgg tcggcttggt ggtaaagcct tcgatgcggt aattggagga
     6241 atgcgcccgc aaaatcgcgc cggttttccg tgtgcgagcc ttttgataat cctggagata
     6301 agtgcgattg gtcgtgccaa cctcaaccag ccgagcccca ctttttgcca ttatatccgg
     6361 taaacgaaaa ctaccgccga tctcgattaa ttcgccgcgc gatacaatga cctcccgact
     6421 atctgccagc gaattcaaag ccagccagac agccgcggca ttgttattga caactgctga
     6481 ggattcggcg ccagtcagca agcaaatttt ttcattggta aggttaagcc gttcgccgcg
     6541 ttcgccggaa tccagatcga attccagatt tacatatccg gaaagtaatt cagccatttt
     6601 tggtaaaatt tcagtcccga aaggcgcgcg tcccagtcca gtatgcagaa cgattccagt
     6661 ggcgttgatt acttttttta aacaacaatt agtcagcgca ttaatccgac cggcaacttt
     6721 ttcggcaacg acttctatca ggtcgtcact cttgggggaa atgaaatttc cagtacggat
     6781 agccgcgcgc agttcattga gctcctggcg gacgagtgtt gtcaggatag ctggtttaat
     6841 tggcgactcc gattgtgcca gacggtcaag gactttttgg actgacggca accgttttag
     6901 ctcgttcgga tagttttctc tacccgccaa aagtttcgcc cttattcatt ttttgcaaat
     6961 tgggtttcat agaaagtgcg gtaaatgcta tcttcgcgac tcaatagtcc gttatgtgtg
     7021 ccgatttcga caattcgtcc gtgctctaaa acaacgattt tatcggcatt atgaatggta
     7081 gaaagccgat gggcgattac cagaacggta cggtttttca tcaaattttc gattgcggct
     7141 tgaacttttt gctcggattc agaatcgagg gatgaagtgg cttcgtcgag aatcaagatt
     7201 ggtggatttt tcagtagagc tcgagcaatt gccaagcgct ggcgttggcc gccggaaagt
     7261 cgcaagcctt tatcaccgat catagtttca aatttttcgg gaaattcttc gataaattcc
     7321 aaggcgttgg cggcttccgc cgcggcacgt actttttccg gatcgaaatc ttgcataccg
     7381 taagcgatgt tgttgaaaat tgtatcgtta aacaaaatcg tatgctgggt aacgattccc
     7441 attaatgaac gcaagctggc aaaagtcaag tcgcgaatat cgtagccatc aatcagaatg
     7501 cggccacctg tgggatcata aaagcgcggc agaagatcaa ccagagtgga tttaccggcg
     7561 ccactatggc caacgatggc gacgatctcg cccttcttga tttccagatt tacaccactg
     7621 agaacctcag tccgagaagt ttcatattta aaagagacat tgtcataaac tatcttgtcg
     7681 ttaaagcctt taatctcgat cgcattcggt ttttctacta cgtccggttt ttcgtcaagc
     7741 aaggaaaaaa cccgttgagc tgatgccaag ccttcttgca tttccatcga aacgttggtc
     7801 agacttttga ttggattcat aatggcaaac agcataacgg cgaaccggac aaaatcttcg
     7861 gcatcaatac ctttcccagt caaaaccaga ctaccaccga accagagcaa aagaacgcca
     7921 ataaatacgc caaaaacctc attaataggc gaagagaatt cacgcagttt cttgcgtcga
     7981 aaaacaaggt taaagtattt acggctttct ttacgaaaac gatcaatctc aaatttttcc
     8041 atagcaaaag ctttcacaac gcgcatacct tggagagtct catccagaat cgccataatg
     8101 ccggcaattt gacgggaaga tcgcagtgat ttgcgtcgaa tactcttgcc gattttggaa
     8161 ataataaaac cactaaccgg caaaatgacc accgccagta atgccaattg ccaggaaata
     8221 atgaaaagca ataccataaa aaccagaata ttaatcggct cgaccagtag tttatcaaaa
     8281 cttaccgtaa aggaattacg catagcgttg acatcgttca ttattattga ggtaatttcg
     8341 ccgcttcgtt tgcggttaaa atatgacagt gaaaggtcat gcaaacgctc gtaaagttta
     8401 ttgcggacat cattaatcat ccgcagttgg acgaaaccga ccgagacatt cttgatatat
     8461 agaaaaacgt ttttcaatag aaaagttaag aaaattatca ggcaaagagc tcgcaaagat
     8521 tcaagtggcg tatctctcag aatcaatcgg tcggtataat ttttcagggt atcgttgaga
     8581 cttcctttgc ctttttccgg aacgacgggt ataacctggc tagtttcggt atcagatgtt
     8641 gccggaaaaa gcactttggt gaaagaagcc gtcagccaga ttgaggtact gttgagagcg
     8701 acataaatca aagaggtaat tatactgact gtcagtaccg cccaataagg cttaactaat
     8761 tttaaaacac gccgataaat gttcataatt gtttcatctt ctcttctatt tcagccatag
     8821 taacggcacc gatccgaatt atttccaatt cacttttacc gactttaacc aacgttgatg
     8881 gtagtgaatc atctgcaatt tcatacttta aaattaatga aatttggtca ccaaagtaac
     8941 tgattatttc ggcggcggaa tgagccggtg gcgcgttagc tggattcacg ctggtggtcg
     9001 ttattgggtg cgggtattgt cgtaaaatgc gattcatcgg ttcgagacta ataacccgga
     9061 cgccgacaga gccgtcagcg gaaatttcaa cagggaaaat gctattcgag ctgagaggca
     9121 aaatcagggt aattttcccc ggtaataagg ttttgacgat tctccgctga tagtcattca
     9181 ggtgtttaaa atcgctcaat agcgacggaa catcggcata tagaacactg atcggttttc
     9241 cagtagtacg acctttcaac tggagtaatt tttgaactgc ttgcggttgg aaggcatcca
     9301 caccaagacc ataaattgta tcggttggat aagcaatgat ggcgccgctt tgcaatgtag
     9361 caagtacttt ttggcagtca atatcggccg ctctggcaag tgaaattatt tcaggcattt
     9421 tgttcaataa aagatgcggg tggtttggtt ttccagcgac ggtgaaacca gtagtattgc
     9481 tcgggacact tgcgaatttg ttcttcaaaa tagattgcga tttgctgagt caaccaggcg
     9541 accggatcgc tcttattctc gggcgggaca tctgtattca attctttcaa cctgattacg
     9601 tatttgcctt tagtattcat caaacaactt gcggcgatta gcggcgcttt ttggcgcaaa
     9661 gcaaataagg ctggaccgcg gtgactggat gaccattgat cgaaaaattt aacaaatatc
     9721 ccatcacgac gggcatcttg atcagctact aaatagagaa tattttcatc gcgcaggtat
     9781 ttccagatac ggggtgaatt cgccttcgat ttgtaaaata tttgctcttt attcctgccg
     9841 ctctgacttc tggcatcaaa aatcatc
//
LOCUS       DAOMJU010000081         9784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_7187_length_9784_cov_19.202487, whole genome shotgun sequence.
ACCESSION   DAOMJU010000081 DAOMJU010000000
VERSION     DAOMJU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7187_length_9784_cov_19.202487"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1638
                     /locus_tag="PLO58_07745"
     CDS             61..1638
                     /locus_tag="PLO58_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HQO74719.1"
                     /translation="MKKLVIGILGIFLMQNLYAQNVENKIVIDVEAGQHMISRHIYGH
                     FAEHLGRCIYGGIWVGEDSPIPNTRGIRNDVVAALKDIAAPNVRWPGGCFADEYHWMD
                     GIGPRDQRPKMINTHWGGVTEDNSFGTHEFMDFCEQVGCEPVITGNVGSGTVREMAQW
                     VEYLTSDNISPMTELRKKNGREKPWRVRFWGLGNENWGCGGNMTADFYADEMRRFSTY
                     CKNYSGNELYRVACGPSDENYEWMETLMKEPINRNCFQGISLHYYTVCHDWTNKGSAT
                     NFNEKDWFLTMSKTLRMDEFVTKHSAIMDKYDPEKKIGLIVDEWGNWHDVEPGTNPAF
                     LYQQNTLRDALVAATNLNIFNNHCERVKMANIAQMVNVLQAIILTNEDQIVLTPTYYV
                     YKMYKVHQDATLLPIELTCQNYELADGSIPAVNASASVDKQGIIHITLCNLDPNKNMN
                     LTCELKSKNKFSVSSGSIITAGKMNAFNDFGKPEEVNLQKFTDCKIAQNTLSVNLPAK
                     SVVMVELVSKSADKPKK"
     gene            complement(1725..>2082)
                     /locus_tag="PLO58_07750"
     CDS             complement(1725..>2082)
                     /locus_tag="PLO58_07750"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQO74720.1"
                     /translation="SGMDLLEGLKEAVSSRKIKNGVILGGIGAVTDYHYHVVSSKNLP
                     PDEEYPKASVPMDLISVQGYILNGRVHAHIALSDANSVVGGHLESGTKALTFFIITIG
                     VLPDSLKIDNLDNYKY"
     assembly_gap    2083..2182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2183..2622)
                     /locus_tag="PLO58_07755"
     CDS             complement(<2183..2622)
                     /locus_tag="PLO58_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74721.1"
                     /translation="MLKIYRRRQFNSTFLVVFSLGLIVLLLNDCTTRPQTSVAQYQFT
                     FEGEVYRIRSIHAVENNERYNELIGKNFLAVDFDQDRIIDKINVGTTTLAEAQKVYDY
                     GLEMLTKENRLKEITPENHKYIEDLEDFHLELKSFYPVNAKPFN"
     gene            complement(2800..4308)
                     /locus_tag="PLO58_07760"
     CDS             complement(2800..4308)
                     /locus_tag="PLO58_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HQO74722.1"
                     /translation="MPASYLTEPRDFHSISLWQQITEKQWHDPIWQRKNAIRDVDELC
                     QVIRLNDYQEGEIAHTIAKLRAEGKEPMRITPYYASLIAEDPFHPNLLPSEEAENRLD
                     PIFWEAVPTPAHLLFPHTGSEGAMAEENRSYGSAYQRYPNRVALFVAENTSCASYCTH
                     CQRLKSLDCTTTISRQDIDKGLFYIDWNPNIDEVLVTGGDALMISPERLEYVLTSLSK
                     IEHLRVIRIATRVPVTLPMAITDDILSLIDKTTSNSGKYVYFMTHINHYHEITPEFKS
                     AIDRIHRHGYSIRNQTVLLKHVNDNLGSLAETLRRMFWAGVEPYYLLQCHRERGLVHF
                     ITPIQIGKIFVKHLQGWISGMARPVYAANIEGGGGKIILMPSGHDTMQKGFNLEEYIS
                     ESYATIHTWDNKIIDDYEALGRATQAEYDAAVKIMDKFIGRKGAFRPSLIIVDKNGNH
                     ITTTNKSPLPSFSNRDKVKIFNYWVDSDDMPLTNPALISDKLDKLFTETTLK"
     gene            4673..5662
                     /locus_tag="PLO58_07765"
     CDS             4673..5662
                     /locus_tag="PLO58_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQO74723.1"
                     /translation="MEQLNDVELLTALTTAHKRLNKEIGRVIVGQQEIINQIVISLLS
                     RGHCLLIGVPGLAKTLMIKTLAQALDLQFRRIQFTPDLMPSDITGTEVIEEDKLTGAR
                     AFRFFKGPIFGNIILADEINRTPPKTQSALLEAMQEHRVTAAGETHQLEEPFFVLATQ
                     NPIEQEGTYPLPEAQLDRFMFSLKVEYPTFQEELEIVKSTTVDADSTPEIVIHRAEIL
                     QFQHLVRRVPVAPNVIEYAVKLVTATRPTNSSAPDFIHKWVEWGAGPRASQYLILGAK
                     ANAVLEGRPTPSIQDVIKMAKPVLRHRIIPNFNAEAEGINTDHIIQKLLETIG"
     gene            5683..6588
                     /locus_tag="PLO58_07770"
     CDS             5683..6588
                     /locus_tag="PLO58_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQO74724.1"
                     /translation="MAEERLDKRRYLEPAVIARLDNMALRARLVVEGFIIGLHRSPYH
                     GFSIEFAEHRPYMPGDEIKHIDWKLYGKTDRFYIKEFEEETNLKSYIILDKSGSMGYQ
                     STAVSKLAYGSYLAAALGYLLLKQQDAVSLTLFDTEITTYLPPVAKKSHLNLVLGALE
                     KVTAGSETSIAPLLHSAAEKIKKRGLIILISDLLDDPSSVINALKHFRHRRHEVIVFQ
                     VLDPQEIEFGFNRQTKFVDLENRDALLTEPGQVRLAYRQAINNFIEDYKIQCRANNID
                     HVLLTTDRPLDLALTEYLNKRARIG"
     gene            complement(6643..7692)
                     /locus_tag="PLO58_07775"
     CDS             complement(6643..7692)
                     /locus_tag="PLO58_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQO74725.1"
                     /translation="MKPRKISFVFVLLLACLFVLDACDPPEITSAKVYLQQNNLQAAE
                     EQLLQAIQKYPDNAQAYLLLGSAIYRPTKRYDEAKQMFEKAKQIDPTKAKEADDNIRG
                     MWAELHTEGANLFNSALKAFLPSEKDSLLNLAAQQFIRALEFKSDEIPTYNGLVKCFY
                     ILNDSANVEKYGKMMLDKNLFDEDVVNYYFQVAWDSTRQEEIFAQLDKIIAEHPDNIE
                     LQILKIQFLSDVGRNEEALDIINKLIENDPTNLDLVYIAAQLLTKLGRFDEAKYQFQK
                     VIANDPTNLELLIRVTEAVFRDKDWVEAEYYARKIIELDPNNVFGYEVLWKSLYNQGN
                     MEEAEKYRMIQKSLE"
     gene            complement(7769..8788)
                     /gene="mtnA"
                     /locus_tag="PLO58_07780"
     CDS             complement(7769..8788)
                     /gene="mtnA"
                     /locus_tag="PLO58_07780"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HQO74726.1"
                     /translation="MIQPLFWENERLLIVDQTRLPFEYKLIEIYDDHEMADAIRRMVI
                     RGAPAIGIAAAYGLALGLKKFILGNHEEFFRQLEIISDRLYETRPTAVNLGWALNRLK
                     RLAELNRHRKLTEIQTILLNEAHTIHTEDIQTCRQIASRGLSLLPDDVRVITHCNAGG
                     LATGGLGTALGIIISAHQSGKKIHVYVDETRPRLQGARLTAWELTQEKVPFTLISDNM
                     AAFVMARNRIDAVIVGADRIAANGDTANKIGTALLAIAAQYHRIPFYVAAPTSSIDPL
                     TPSGAAIQIEERDGTEVKEINGQPIAPVGCSAISPAFDVTPARLITAIITEKGIYQPP
                     YRLIE"
     gene            complement(8810..9715)
                     /locus_tag="PLO58_07785"
     CDS             complement(8810..9715)
                     /locus_tag="PLO58_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQO74727.1"
                     /translation="MKKSILVVGSVAFDDIETPVGKRFNALGGSAVYFSLAASQFSQV
                     NLVGVAGEDYPKAAIDLLNRQGIDTTGLEIAPGPTFRWGGRYHQDLNNRDTLYTHLNV
                     FANFSPKIPSVYQKTPVVFLGNIQPTLQMEVLNQLPQPEFIGLDTMNLWINTTRAELM
                     KIISRVNLLVINDSELEELTGTVNLFKGLKYLHKIGVRHIVIKQGEYGAMLSAAEGLF
                     FTPAYPVEEVVDPTGAGDSFAGGLFGYLGTCEKIDYNNLKKAVVYGSVMGSFCVENFS
                     IDGLLNLNPSKIAKRFRELRRLVKA"
BASE COUNT         2780 a         2028 c         2100 g         2776 t
ORIGIN      
        1 aaaatttgca gagaagaaaa gaataaataa atttataacc aaatgtaaag gagtatcgtt
       61 atgaaaaagt tagtcatcgg cattttgggt atatttctta tgcagaattt gtacgcccag
      121 aacgtcgaaa ataagattgt cattgatgtc gaggccggcc aacatatgat cagtcggcac
      181 atctatggcc atttcgctga acatcttggt cggtgtattt acggcgggat ttgggttggg
      241 gaagattcac caattcccaa tactcggggc attcggaatg acgtggttgc cgccctgaaa
      301 gacattgccg cgccgaatgt gcgctggcca ggtggttgtt tcgccgatga atatcactgg
      361 atggatggca ttggtccccg cgaccaaaga ccgaaaatga tcaataccca ctggggcggc
      421 gttaccgaag ataatagctt cggtacgcat gaatttatgg acttctgcga gcaggtcggc
      481 tgtgaaccgg taattaccgg taatgtaggc agtggcaccg tgcgtgaaat ggctcaatgg
      541 gtcgagtatc tcacttctga taatattagt cccatgaccg aactgcgcaa aaagaacggt
      601 cgcgaaaaac cctggcgcgt tagattttgg ggattgggaa atgaaaactg gggctgtggt
      661 ggtaatatga cggcggattt ctacgccgat gagatgcggc gtttttcaac ttattgtaag
      721 aattacagcg gcaatgagtt atatcgagta gcctgtggcc catctgacga aaactacgaa
      781 tggatggaaa ccctaatgaa agaaccgatc aatcggaatt gctttcaggg aatttctttg
      841 cattattata cagtttgtca tgactggacc aataaaggtt ctgctaccaa ttttaacgaa
      901 aaagactggt ttctgacgat gtcgaaaacg ctccgaatgg atgagtttgt caccaaacac
      961 tctgccatta tggataaata tgatccggaa aagaaaatcg gactcatcgt tgatgaatgg
     1021 ggtaattggc acgatgtcga acccggcact aatccggctt ttctatatca acaaaataca
     1081 ctcagagatg ctctggttgc cgccactaac ctgaacattt ttaacaacca ctgcgaacga
     1141 gtgaaaatgg ctaacattgc ccaaatggtc aatgttttac aggcaataat tttgacgaat
     1201 gaagaccaga ttgtcttgac gcccacttat tatgtttata aaatgtacaa agtgcatcag
     1261 gatgcaactt tattgccgat cgaattgacc tgccagaatt atgaactggc tgacggttca
     1321 attccagcgg tgaatgcctc agcttccgtt gataaacagg gaattattca tattacgcta
     1381 tgcaatctcg atccgaataa aaacatgaat ctgacctgtg aattaaagag taaaaacaaa
     1441 ttttcggtaa gcagtgggtc aattatcaca gctggtaaga tgaacgcctt caatgatttt
     1501 ggtaaaccgg aggaagtcaa tctgcaaaaa ttcaccgatt gcaaaatagc ccaaaatact
     1561 ctttcagtta atctgccagc caaatcggtg gttatggtcg agctggtgag taaatcagct
     1621 gataagccga agaaataaaa gactgattgt ttatcgttaa aattggtcgg aatcagacaa
     1681 aaggttgact gaattttccg aacgaatatt tattttcatt cggattaata tttataatta
     1741 tcaagattgt cgatctttaa actatcaggc aacaccccga tagtaataat gaaaaaagtc
     1801 agagcttttg tgcctgattc gagatgaccg ccaacgacac tgtttgcgtc cgacagtgcg
     1861 atatgagcat gaacccgtcc attcagaata taaccttgca cggaaattag atccattggc
     1921 actgaggctt ttggatactc ttcgtccggc ggtagatttt tactgctaac gacgtggtaa
     1981 tgataatcgg tcacggcgcc gattccgcct aagataacgc catttttgat ttttcgactg
     2041 ctaacggctt cctttaatcc ttctaataaa tccattccag agnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nntggttgaa cggtttagca ttgactggat aaaaactttt
     2221 taattcgagg tgaaagtctt ctaggtcttc aatatattta tgattttctg gggttatttc
     2281 cttaaggcga ttttccttgg tcagcatttc aagaccataa tcgtaaactt tttgtgcctc
     2341 cgctaaagtg gtggtcccga cgttgatttt atctataatc cgatcctgat caaagtcaac
     2401 agctaaaaaa ttcttaccaa ttaattcgtt gtagcgttca ttattttcga ccgcatgaat
     2461 cgagcgaatg cgatagactt cgccctcaaa ggtgaattga tactgagcca cgctggtttg
     2521 cgggcgagta gtgcaatcat taagaagcag tacaattaaa ccgagactaa aaacaaccag
     2581 aaaagtcgaa ttaaattgcc tacggcgata aatctttaac atgacttttt ctccttttaa
     2641 aagatttgtt cttcatttat tttattatca aagactaaat cacatttaaa tttaaagtaa
     2701 atgtttctca aagaaatata aatattttgc ggagaaaact aactgtaatt ttagccggtg
     2761 aattagttag agtgctacaa atagcccgac tgattttaat tacttcaagg ttgtttcggt
     2821 aaataactta tcgagtttat cgctaatcaa agccggatta gtcaaaggca tatcgtcaga
     2881 atctacccaa taattaaaaa ttttaacctt atcgcgattg ctgaaacttg gtaaaggact
     2941 cttattagtt gtcgtaatgt ggttgccatt tttatcaaca ataatcaaag atggtcggaa
     3001 agctcctttc cgcccgataa atttatccat tattttgacc gccgcatcat attcagcctg
     3061 agtagcgcgt ccgagtgctt cgtaatcgtc aataatctta ttatcccagg tatggatcgt
     3121 tgcatagctt tctgagatat actcctcgag gttaaaacct ttttgcatgg tatcgtgtcc
     3181 agaaggcatc agaattattt ttcctcctcc accctcgata ttggcggcgt aaactggccg
     3241 agccattccg cttatccaac cctgaagatg tttcacaaaa attttgccaa tttgaattgg
     3301 cgtaatgaag tgcaccaaac ctcgttctcg atgacattga agtagatagt aaggttctac
     3361 tccagcccag aacattcgtc gcaaggtttc ggcgagtgaa ccgaggttat cattgacatg
     3421 tttcaacaaa acggtttgat tgcgaatcga atagccatgc cggtgaatgc ggtcaatcgc
     3481 ggatttgaat tccggagtaa tttcatgata atgattaatg tgagtcatga agtaaacgta
     3541 ttttccggag ttagaagttg ttttatctat aagagataat atatcatcag taatagccat
     3601 cggcaacgtt actggaacgc gagtagcgat ccttataacc cgcaaatgtt caatttttga
     3661 taaactggtt aagacgtatt cgaggcgttc gggactaatc atcaaagcat cgccaccggt
     3721 cactaaaact tcatcaatat taggattcca atcaatataa aatagtccct tgtcaatatc
     3781 ttgccgactg atagtagtag tacagtcgag tgattttagt ctctgacaat gggtacagta
     3841 agaagcgcag ctggtatttt cggcaacgaa gagcgcaact ctattaggat aacgttgata
     3901 ggcagaccca taactgcgat tttcttcagc cattgcacct tcacttccgg tgtgggggaa
     3961 taatagatga gccggagtag gaacagcttc ccagaaaata ggatccagcc gattttcggc
     4021 ttcttcgcta ggtaatagat tggggtggaa aggatcttcg gcaattaggg acgcataata
     4081 aggtgtaatg cgcatcggtt ctttgccttc cgcacgtaat ttggcgatag tatgggcaat
     4141 ttcaccttct tgataatcgt ttagccgaat cacctgacat agttcgtcaa cgtcacggat
     4201 ggcatttttc ctctgccaga tgggatcatg ccattgcttt tcagtaatct gttgccagag
     4261 tgaaattgaa tgaaaatcgc gtggctcggt taaataagat gcgggcatcg ttaatttctc
     4321 ctaagtttta tgtagcttaa attattataa aatgtcatta ttcttttgga atctaaaaaa
     4381 cagtcgtttt ttatgcaaat cctacttgct tatataaaat cgagagcctt gccagtgcag
     4441 caggaaattt ttgggattaa aatgagaagc tgattagaaa ttattatgcc agaccccact
     4501 acgtttatat aaatttatat tgggttaaaa ttctaatgta ccgggctgac gctcagggag
     4561 aatttatcaa attggttatt tatcatctaa caggcaccgg caattattgg caaattgagc
     4621 ggaaagtctt agattctcca cgctttcaat tttataatcg gaatggaaat atatggaaca
     4681 gcttaatgat gtcgaattgc tgacggcttt aaccacagct cataagcggc ttaataaaga
     4741 aattgggcga gtgattgtcg gccagcagga aattattaac caaattgtga tttctctcct
     4801 ttcgcgggga cattgtcttt tgattggtgt gccgggatta gccaagacgc tgatgattaa
     4861 aaccttagcg caggcgcttg acctgcaatt caggcgtatt caatttacgc ctgatttaat
     4921 gccgtccgac attaccggca ccgaagttat cgaagaagat aagctgaccg gcgctcgcgc
     4981 ttttcgattt ttcaaagggc cgatttttgg caatattatt ctcgctgacg aaatcaaccg
     5041 cactccacct aaaacccaat ctgctttgct ggaagcgatg caggaacatc gcgttaccgc
     5101 cgccggtgaa acgcaccaat tggaggagcc gttttttgta ttagcgactc agaatccgat
     5161 tgagcaagag ggcacctacc cgttgccaga agcgcagctt gatcgtttta tgtttagttt
     5221 aaaggttgaa tacccgacct ttcaagaaga actggagatt gtcaagtcaa ccacagttga
     5281 tgccgactcc acaccggaaa ttgtcattca tcgagccgaa attctgcaat ttcagcatct
     5341 tgttagacga gtgccggtgg ctccgaacgt tattgaatat gcggtgaagc tggtaactgc
     5401 tacgcggcct actaattcca gcgcgcctga ttttattcac aaatgggtag aatggggagc
     5461 aggaccgcgt gccagccagt atttgattct cggcgctaaa gcgaatgccg ttctggaagg
     5521 tcgtccaact ccgtcaattc aagacgtaat taagatggcg aagccggtgc tgaggcatcg
     5581 tataattccg aactttaatg ccgaagccga gggaattaat accgatcaca ttattcaaaa
     5641 gttactcgag actatcgggt aatctgggga atgtttttat aaatggcaga agaaagactg
     5701 gataaacggc gttatcttga accggcagtc attgctcggt tggataatat ggctttgcgc
     5761 gccagactgg tagtggaagg ctttataatc ggcctccatc ggagtcctta tcatggcttt
     5821 tcgatcgaat ttgctgaaca ccggccttat atgccgggcg atgaaatcaa acatatagac
     5881 tggaagcttt acggtaaaac cgatcgcttt tatataaaag aattcgaaga agaaaccaat
     5941 cttaaatcct atattattct cgataaaagc ggttcgatgg gctaccaatc aaccgccgtt
     6001 tccaagctgg cttatggcag ctatttagcc gccgcgctgg gatatttatt acttaaacaa
     6061 caagatgcag ttagcctgac tctttttgat accgaaatta ctacctactt accacccgtc
     6121 gctaaaaaga gtcacttgaa tttagtactc ggagctttgg agaaggtcac cgccggttcg
     6181 gaaacttcca tagccccact cctgcattcc gccgctgaaa agattaagaa acgcgggctg
     6241 attattctta tttcagattt gttggacgac ccttcaagcg taatcaatgc cctgaagcat
     6301 tttcgccatc gccgtcacga ggtaatcgtg tttcaagttc ttgaccctca agaaatcgag
     6361 tttggtttca atcgccagac taaattcgtt gatctggaaa atcgtgacgc gttattgact
     6421 gaacccggtc aggtgagatt agcatatcgg caggcgatca ataattttat tgaggattat
     6481 aaaatccaat gccgggcgaa taatattgat catgtcttat taactaccga ccgccctctg
     6541 gatttagcct tgaccgaata tttaaataaa cgcgcccgaa ttggttagaa aacaaaaaag
     6601 ccggatcttt agtccggctt tttaaaaaat taatcagcag gcttattcca gcgatttttg
     6661 tatcatacga tacttctcag cctcttccat attaccttga ttatataacg atttccagag
     6721 aacttcatac ccgaatacat tatttgggtc cagctcgata attttacgag cgtaatattc
     6781 cgcttccacc cagtccttat ctctaaaaac cgcttcagtt acgcggatta gtaattccag
     6841 gttagtggga tcgttagcga taaccttttg aaattgatat ttagcttcat cgaatcggcc
     6901 gagcttagtc agtaattgag cggcgatgta aaccagatcg agattggtgg gatcattttc
     6961 gattaattta ttaattatat caagggcttc ttcattgcgt cccacgtcgg ataggaactg
     7021 gattttaaga atttgcaatt caatattgtc cggatgttca gcgataattt tatctaactg
     7081 agcgaaaatt tcctcctgac gggtgctatc ccaggcaact tgaaaataat aattgacgac
     7141 atcctcatcg aaaagatttt tatccagcat cattttgcca tatttctcga cattagcaga
     7201 gtcattcaga atataaaaac attttaccaa gccgttataa gttggaattt catcggactt
     7261 gaattccaat gcacgaatga actgctgagc ggcaagattc aagagcgagt ctttttcaga
     7321 cggtagaaaa gcttttaaag cgctgttgaa caagttggcg ccttcagtgt gtaactcagc
     7381 ccacattcct ctaatattgt catcagcctc ttttgccttg gtaggatcga tttgcttggc
     7441 tttttcgaac atctgtttgg cttcatcata gcgtttagtc gggcgataaa ttgcgctacc
     7501 caataagaga taggcttggg cattatccgg atatttctgg atagcttgca gtaattgttc
     7561 ctcggcggct tgcagattat tctgctggag ataaacttta gccgaagtaa tttccggcgg
     7621 atcgcaagca tccagaacga acaaacaagc cagtaataag acaaaaacaa aagaaatttt
     7681 tctcggtttc attattctta ctcctttatc aatctgaata ttttttccaa atcacattaa
     7741 aatttacaaa aatgctcaca aaattcaact actcaattaa acggtagggc ggctggtaaa
     7801 tgcctttttc agtaattatt gctgtaatta gccgtgccgg agtaacatcg aaagccggtg
     7861 aaattgccga gcaaccgact ggggcgattg gttggccatt tatttccttg acttcagtac
     7921 catcacgttc ttcaatctga atcgccgcgc cggatggagt gagtggatca atgcttgaag
     7981 taggcgcggc cacataaaac ggaatccggt ggtattgagc cgcgatagcc agtagagccg
     8041 ttccgatttt gttagccgta tcaccattag ccgcaatccg atcggcgccg acgataaccg
     8101 catcaattcg gtttcttgcc ataacgaagg cggccatatt atcgctaatc aaggtaaagg
     8161 gcactttttc ctgggtgagt tcccatgccg tcagacgagc gccctgcaac cttgggcggg
     8221 tttcatccac ataaacgtga atttttttgc cactctggtg ggcactaata attatcccta
     8281 aagcggttcc cagtccgccg gttgccagcc cgccggcatt gcaatgcgtg ataactcgaa
     8341 catcatcagg tagaagtgac agaccacgag aagcaatttg acggcacgtc tgaatatctt
     8401 cagtatgaat ggtatgggct tcgttaagta aaatggtttg aatttcagtt aatttccggt
     8461 ggcgattgag ttcagctagt cgtttcagac gatttaatgc ccaacccagg ttgacggcag
     8521 tcgggcgggt ttcatacagt cggtcagaaa ttatctctaa ctgacggaaa aattcttcat
     8581 gattacctaa gataaacttt ttaagcccca gcgctaaacc ataggcggcg gcaatgccga
     8641 tagctggagc gccacgaata accatacggc gaatcgcgtc cgccatttcg tggtcgtcat
     8701 aaatctcgat cagtttatat tcaaacggca ggcgcgtctg atctacaatc agcaggcgtt
     8761 cattttccca aaataagggt tggatcataa gcaattagtc cgaatgagct caggctttca
     8821 ctaaccggcg caattcgcga aatcgctttg cgattttgga agggttaaga ttcaataaac
     8881 catctatact gaaattttcg acacagaatg aacccataac actgccgtaa accactgcct
     8941 tttttagatt gttgtaatca attttttcgc aggtgccgag ataaccaaac agtccacccg
     9001 caaaactatc accggctccg gtaggatcta caacctcttc aactggataa gccggagtga
     9061 aaaataagcc ttcagccgcg ctgagcattg caccgtattc tccctgcttg ataacgatat
     9121 gccgtactcc gatcttatgc aagtatttta aacctttgaa caggttaact gtaccagtca
     9181 attcctccag ctcactatcg ttgattacca gaaggttaac tcgcgatatg attttcatta
     9241 gttcggcgcg cgtggtatta atccacaaat tcattgtatc cagtccgatg aattccggct
     9301 ggggtagttg attaagcacc tccatttgta aagtcggctg aatgttgcca aggaagacga
     9361 ccggtgtctt ttgataaacg gagggaattt tcggactgaa attagcgaag acattaagat
     9421 gagtataaag agtgtcgcgg ttattgaggt cttggtgata acgcccgccc cagcgaaaag
     9481 tcggtcctgg agcaatttct aaaccggtag tatcaattcc ctgacggttc agcaggtcaa
     9541 tggcggcttt ggggtaatct tcaccggcga cgccgactaa gtttacttgt gagaattgag
     9601 aagccgctaa actgaaataa acggccgaac cacccagggc attgaaacgc tttccgactg
     9661 gggtttcgat atcatcgaaa gcaacggaac caactaccag aatagatttc ttcaaaacta
     9721 tttccttatt ttattatcaa ttaaccaccg aagctaaaaa tactttggcg cttttgcaat
     9781 atgg
//
LOCUS       DAOMJU010000082         9652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_7336_length_9652_cov_21.028134, whole genome shotgun sequence.
ACCESSION   DAOMJU010000082 DAOMJU010000000
VERSION     DAOMJU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7336_length_9652_cov_21.028134"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLO58_07790"
     CDS             <1..608
                     /locus_tag="PLO58_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74728.1"
                     /translation="RLFSAQFLRLNLMNCTNYQQFNVLNHKAIHKIFSWPLDTSLQES
                     ESTKFARVLICLPFEHAHFYSALKVFLPFIRQKPELVRLLVPQAFFTLIQEINPDLLI
                     PLISSRINKEGFPTDPEVLALARINLEFAIDLNVVPTVPTAFIVSRCRAPIKVGFQSE
                     LAEKLFNLIINYKTDRLVEKSYNLIWDLLQPQIQKVNKNLT"
     gene            661..1077
                     /locus_tag="PLO58_07795"
     CDS             661..1077
                     /locus_tag="PLO58_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74729.1"
                     /translation="MEDLVCPECNAPLKEEDLKQSLVCPNCKTNLRDSKYLDFLELLV
                     YYDIIDDIDFFDMSLYGKEMLEHEREDFDEEEIDPSKFEKKKEVWDEFEDDLELNENL
                     TEEIESSDDAWDLSDTDIPFEDEWDEDNEDREEDEY"
     gene            1107..1430
                     /locus_tag="PLO58_07800"
     CDS             1107..1430
                     /locus_tag="PLO58_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HQO74730.1"
                     /translation="MNSTKPVAQIKIELPESKAEGNYSNFILISHSSAEFVLDFARIM
                     PGSPKAVVQSRIILTPIHAKTLLFTLQDNLAKFEAKFGEIKLPDKESFEPFGFPNSEE
                     SKLPN"
     gene            complement(1454..3493)
                     /locus_tag="PLO58_07805"
     CDS             complement(1454..3493)
                     /locus_tag="PLO58_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQO74731.1"
                     /translation="MKKLITIIFTVIVLATSAKPQDLLTVFTEPYLSGSVAANVTWSP
                     NDSLIAFLWNDQGGEFRNLWIANPQTGAKEQLTDFNPTTNVNLPTIGTLGWLNNRCLF
                     FEYQGDIWEASLLPGYPLKRIEGLTNCKPPIALAPNRRYLTYNRGGYLRLFDFITKEE
                     QTIIPDKAYQRPPARQVCAYQTTYCWSNDSKKVAVYLESEADENTVHFKVFTVPKSST
                     DDVVIKEHPNDNSIVRNFIWSPDNQSIVIESVTSNLRERYILLVDLTKDRLDTLYREI
                     SNNWIADFGYQLYWIESEAKILFGMEYNSYNHLFLLDPTNGHYSALTRGKWDVKDYAV
                     DATGQLVYFTATKDNPEQLQLYSAQIKTGEITDISYRGGDYSFQLSNNSRWIAEVFSN
                     YSTPPDLYWIEASPQSKMNRITVRPDSPLGKPLRKIPKSGTIQNAITGKPVNYRVWLP
                     DDFRVNHKYPIIVVLNGADCVAPLTEAWRPECYFKQWLSDKGYIVVAVQYTDLRTAIE
                     PADSESFSDPLKIQLADIQAVVKEIGKLQYVDLSRSGICGWGYGGYLATMAMFETDNI
                     FRAGVAILNDFSWSQSSSIYLNYMANLLANDQVFRKIDPHNFCYNLRGRLLVIQSASS
                     TLPQLVDAQRLIPELLSIRRPVDFYLYPWENNPIMRSANQYDMFIKTARFFQLNL"
     gene            complement(3521..4189)
                     /locus_tag="PLO58_07810"
     CDS             complement(3521..4189)
                     /locus_tag="PLO58_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQO74732.1"
                     /translation="MNLSKPTENRPIYAKTWQNVAAVERYALSRYRHYDQRLISWREK
                     RIVNHLLKQIKNGNHILLDIPSGYGRFTPLFRQHGFKVINGDLNLYALLYQRQRFPDS
                     NLMFVTDGNCLPLPDNSAGVVFNFRLLQHFQTSDQRIALLRELARVTNCYLIVSVYLE
                     SNFHRLAQLVSRRKRRMTMISRTQWDTELRSCGLKILRISRPLRFLHAQNIFLLEKSP
                     IIEK"
     gene            complement(4452..5123)
                     /locus_tag="PLO58_07815"
     CDS             complement(4452..5123)
                     /locus_tag="PLO58_07815"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQO74733.1"
                     /translation="MILRKKPEASLKLRQPLMYRVGLVVALVLITGIMIAFPKFEERE
                     VVQRKVDVILEVTDIPQIEQKIEAAKPPSRPSIPIESENEDLSEDITIDQTTDFDSYT
                     AWEAPPPPPEQHSDEGEGPRVRFIPYDEPPEPIGGFAEIQKNIVYPEIAQEAGIEGTV
                     VVQAYVNEYGVVTECTILKGMPNTGLEEAAIAAIKKTRFKPAKQRDRNVGVYISIPVI
                     FKLKS"
     gene            complement(5134..5541)
                     /locus_tag="PLO58_07820"
     CDS             complement(5134..5541)
                     /locus_tag="PLO58_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQO74734.1"
                     /translation="MAFQHKKNISTEVPTASMPDIIFTLTLFFMATTVMRQYSGLPVV
                     LPKAKQIEKLQSKIHVTELWVSKEELISIDGKLVNLQDIRNIMYNKRVRDPQLIVSIR
                     ADERVTMDLMSKIHKELRLADALKVNYSTKTAS"
     gene            complement(5553..5951)
                     /locus_tag="PLO58_07825"
     CDS             complement(5553..5951)
                     /locus_tag="PLO58_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQO74735.1"
                     /translation="MALLKKKKTRKVDIPSASQADIAFLLLLFFICTTTVDVDKGIGL
                     ILPPEGDSPVEISKKNITTIVIDPQGNVLIDNKEVEINQIRDIAERLQMENPKMIFSV
                     KAHPRCRYDKYVAVLDQLKMAKATRISIAE"
     gene            complement(5954..6577)
                     /locus_tag="PLO58_07830"
     CDS             complement(5954..6577)
                     /locus_tag="PLO58_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQO74736.1"
                     /translation="MVELFLRGGIFMWPILGLAIFGIMVVIERAYTLSKASRITKRFL
                     PKVLNTLKEEDGVKKAIELCENTPGPIAEIFHAGLSAVGNGMEAVEKAIQSAGTVEMA
                     FLDKGMIWLATVITLAPLLGFMGTVSGMINAFDAIAKVNDMSPAIVAGGISEALLTTL
                     FGLIVAAPLQLCYNLFVARVDSIVVEMEESTMNLLQTLDVINKENCK"
     gene            complement(6678..8267)
                     /locus_tag="PLO58_07835"
     CDS             complement(6678..8267)
                     /locus_tag="PLO58_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQO74737.1"
                     /translation="MRKATIPVIIIIVVLGFLFISPFGKSIIASGSDLYQKLNIFQDI
                     IRIINDSYVEEPDWNKVMEGAYRGMLEELDPHSVYIEPKQLDDLNEQFSGKFEGIGIE
                     FDILDGYITVISPVAGAPAERAGLEVGDKIVKIDGESAYRISRDDVQKKLRGPKGSEV
                     TVTVRRQGQTDFDVKIIRDEIPLYSVSAAFMIDDSTGYIFLNRFSGTTSDEVEKALNR
                     LAAEGMQRLVFDLRNNSGGYLEQAVEIADKFIAGRQKIVYTRGRISSANEEFYSSKTG
                     PYEKIPLVILINRGSASASEIVAGAVQDLDRGLIVGETSFGKGLVQRQYPLRDGSAIR
                     VTVARYYTPSGRLIQRPYDGAIDDYYDTFSAENRDSLLAVKDSVEKRPKFSTIGGRTV
                     YGGGGITPDYSVKYDQALNNETYKLLRHPSRVIFEYASDFVNKNRDLAADRKNFYKNF
                     EVSNKDFEAFKKMAVEKIGDLNIKQVEKDAKYLKILIKAEIARGYWGYDEYYKIVRLS
                     DNQVTSAVDYLGEARKMLQIQ"
     gene            complement(8374..8850)
                     /locus_tag="PLO58_07840"
     CDS             complement(8374..8850)
                     /locus_tag="PLO58_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQO74738.1"
                     /translation="MKSPENKDKFMREGLEYIKQIVNRRGSRDTKLLEICRFLNDKFI
                     GFDWVGFYFSDNLKKELILGPFIGDPTEHRNIPFGRGVCGRAAVSGTTVNVADVTAES
                     NYLACSLEVRSEIVIPIFKSGQFVAELDIDSHQSDRFGNSERQFLEQVGQIISVIF"
     gene            complement(8909..>9652)
                     /locus_tag="PLO58_07845"
     CDS             complement(8909..>9652)
                     /locus_tag="PLO58_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQO74739.1"
                     /translation="HNLGIHTMYGPIDVGVRVEFPDDIYRPIAAVMYDAKFRLYSKSY
                     DDLVRTFCTNPSGYIVTEKYDDFVLVNGHAKWNSKTDNTNFALLSRVVLTDPVEDTTK
                     YGRDIARLATTIGGGKPIIQRLTDFVSGRRSNWERISRSATVPTLKDVTPGDIAMAFP
                     HRIVTNLIEGLTVLNKIISGLMSNNTLLYAPEIKFYDTKYKVTPYLETTLENFFVAGD
                     ASGHSRGIVYSAVTGIFAAQGVLKNLGKD"
BASE COUNT         2888 a         2124 c         1819 g         2821 t
ORIGIN      
        1 aaaggttatt ttcggctcag tttttaaggt tgaatttgat gaattgtact aactatcaac
       61 aatttaatgt cttgaatcat aaggctattc ataaaatatt tagctggcct ctggatacgt
      121 ctttacagga atcggaatca actaaattcg ctcgagttct aatttgctta ccctttgagc
      181 acgctcattt ctatagcgca ctgaaagtct ttttaccttt catacgacaa aaaccggagt
      241 tggtcagatt gctcgtaccg caagcatttt ttaccttaat tcaggaaatt aatcccgatt
      301 tattgatacc cttgatttcc agtcggatca acaaagaggg ctttcccacc gatccagaag
      361 tactggcatt agctcgaatc aacctcgagt tcgcaattga tttaaatgta gttccgacgg
      421 ttcctacggc ttttatcgtt tcccgctgcc gcgcgccgat caaggtcggt tttcaaagcg
      481 aattagcgga aaaattattc aacctaatca ttaattataa aacggataga ttagttgaaa
      541 aatcctacaa tcttatttgg gatttattac aaccgcaaat ccaaaaagta aataaaaatt
      601 taacttgacg catatacctt cggcgtgtat atttagcgcc gtaatataag gtgaaattat
      661 atggaagatt tagtttgccc cgaatgtaat gcgcctctca aagaggaaga tttaaaacaa
      721 tctctggttt gcccgaattg caagactaac ctgcgggatt ccaaatatct ggattttctg
      781 gaactgttgg tctattatga catcattgat gatattgatt tcttcgatat gagcctgtat
      841 ggcaaagaaa tgctcgaaca cgaacgcgag gatttcgacg aagaagaaat tgatccatcc
      901 aaatttgaga aaaagaaaga agtctgggat gaattcgagg acgaccttga gttaaatgaa
      961 aatctcactg aagaaattga aagcagtgat gacgcctggg atctctcaga tactgacatt
     1021 cccttcgaag acgaatggga cgaagacaat gaagatcgcg aggaagatga atattaactc
     1081 cctcgtatca ttttaaggaa agattaatga actcaactaa acctgtcgcg caaattaaaa
     1141 ttgaattgcc agaatccaag gcagaaggta actacagtaa ttttatactc atttcgcact
     1201 cgagcgccga atttgtactt gatttcgcac gtatcatgcc cggctcaccc aaagccgttg
     1261 tgcaaagccg aataatcctg actccaattc acgccaagac gttactattc actttgcagg
     1321 ataacctcgc caagttcgaa gccaaatttg gtgagatcaa actcccggac aaagaatctt
     1381 ttgaaccttt tggatttccc aattcggaag aaagcaaatt gcctaattga acaacctcat
     1441 ttctgagcca gagtcataaa ttcaactgga aaaagcgcgc cgttttaata aacatatcat
     1501 attgattggc gctcctcata atcggattat tttcccaagg ataaagataa aaatctaccg
     1561 gtctacggat agataacaat tcaggaatta accgctgggc atctactaat tgaggtaaag
     1621 tgctggatgc gctctgaata acaagcaaac gtccccttag attatagcaa aaattatgtg
     1681 gatcaatttt tctaaaaacc tggtcattag ctagtagatt agccatataa ttcaaataga
     1741 tactcgatga ttgtgaccag gagaagtcat ttaaaatcgc aaccccggct ctaaagatat
     1801 tatcagtttc gaacatagcc atcgtggcta agtagccacc atagccccaa ccgcaaatcc
     1861 cgcttcttga caaatcaaca tattgaagtt taccaatttc tttgacaaca gcctgaatat
     1921 ctgctaattg aattttcaaa ggatcgctga acgattccga gtccgctggt tcaatagctg
     1981 tccttaaatc cgtatattgc acagcaacaa caatataacc tttatcactc agccattgct
     2041 taaaataaca ttccggtcgc caggcttcgg tcagcggcgc tacgcaatca gcaccattta
     2101 atacaacgat aatcgggtac ttatgattta ctctaaaatc gtcgggtaac catacgcggt
     2161 aattaactgg tttaccggtt attgcattct gaattgttcc actcttcgga attttcctca
     2221 gcggtttccc gagtggtgaa tctggacgaa cagttattcg gttcatttta ctttggggag
     2281 acgcttcgat ccaatataaa tcgggcgggg tagagtaatt ggaaaaaacc tccgctatcc
     2341 aacgactatt attggataat tggaatgagt aatcaccacc tcgataagaa atatctgtaa
     2401 tctcacccgt tttgatttgg gcagaatata attgcaattg ttcaggattg tcttttgtcg
     2461 ctgtaaaata taccaactgc ccagtagcat ccacagcata atccttaacg tcccatttgc
     2521 cacgagttaa ggcggaatag tgcccattag tcggatcaag cagaaataaa tgattataag
     2581 agttatattc catcccaaac aaaatcttcg cttccgactc tatccaatat aattggtatc
     2641 cgaaatccgc aatccaatta ttgctaattt cacgatataa agtatccaat cggtctttcg
     2701 tcagatcaac taaaaggata tatctttcac gtaaattaga agtaaccgat tcaataacta
     2761 ttgactgatt atctggcgac cagataaagt tgcgcacaat ggaattgtca ttcggatgct
     2821 ccttaatcac aacatcatcg gtcgagcttt tcggtacggt gaaaacttta aaatgaacag
     2881 tattttcatc ggcttccgat tctaaataga ccgcaacctt tttactatca ttcgaccagc
     2941 aataagtagt ctggtaagcg cacacctgtc tggcgggtgg acgctgataa gctttatccg
     3001 gaataatcgt ctgttcttct ttagttataa aatcaaacaa tctaagatag ccacctctgt
     3061 tgtaggttaa atatcttcta ttcggcgcaa gagcaattgg tggtttgcaa ttcgttaagc
     3121 cttcgatccg ttttaatgga taacctggca acagacttgc ctcccaaatg tcgccttgat
     3181 actcaaagaa aagacaacga ttatttagcc aaccgagcgt tcctatagtc ggcagattta
     3241 catttgtagt cggattaaaa tcggtcagct gttctttcgc acctgtttgc ggattagcaa
     3301 tccagagatt gcgaaattct ccgccttgat cattccacag aaaagcaatc agggaatcat
     3361 tcggcgacca cgttacgtta gccgctaccg agccggaaag ataaggctca gtaaatacag
     3421 tcaacaaatc ttgcggcttg gctgaggttg ctaatactat gacggtgaat ataatcgtta
     3481 ttaacttttt catccttcac ctcaatttat aagcagttaa ttatttttca attattggcg
     3541 atttctccaa taggaaaatg ttttgagcgt ggagaaaacg aagcggacga ctaatccgga
     3601 gtatttttaa tccgcacgaa cgcaattcgg tatcccactg agttcgagaa atcatagtca
     3661 tccgacgttt acgccgtgaa actaattgag ctagacgatg aaagttcgat tcaagataaa
     3721 ccgatacgat caggtaacaa tttgtgaccc gcgcaagctc tcgtaagagt gcaattctct
     3781 ggtcactggt ctggaaatgt tgaagaagtc tgaaattaaa aacgacaccg gctgagttat
     3841 caggcaacgg taaacaattg ccatcagtta cgaacattag gttactatcg ggaaaacgtt
     3901 gccgttgata aagtagagca tacaaattaa ggtcgccatt gataaccttg aaaccgtgtt
     3961 ggcgaaagag aggggtaaag cgtccgtaac ccgaaggaat atctaacaat atgtgatttc
     4021 cattttttat ttgttttaga agatgattaa cgatccgttt ttcccgccag gaaattaagc
     4081 gctgatcata atgacgatag cggctaagag catagcgttc aacagcggct acattctgcc
     4141 aagtcttggc ataaatagga cggttctctg taggtttact taaattcact tagtcccgtt
     4201 gactttgatc taaatcttca attcatttta cacagaaagt ccaaatttgg caaatgattt
     4261 aaacgaaaaa aacgagtaat gaagaaaaac attcaataga ctcttggtat cgttaataaa
     4321 tagataaaaa ccaaggagat tgtcttcacc aatgaagcaa attaaccagt cagaatttcg
     4381 actaataatt aaaaaccaaa accggcataa ttgagattat gccggtcagg taaatattta
     4441 ggactaattg attagctttt tagtttgaaa ataaccggga tcgaaatata gacaccaacg
     4501 tttcggtctc tttgtttagc cggtttgaag cgggtttttt tgatcgcggc gatagcggct
     4561 tcttcgagtc ccgtattggg cattcctttt agaatggtgc attcggtaac gactccgtat
     4621 tcattcacat aagcctggac tacaaccgtc ccttcgatac cggcttcctg cgcaatttct
     4681 ggataaacta tgtttttctg aatctcggca aatccgccaa tcggctcggg aggttcatca
     4741 tagggaataa atcggacgcg cgggccttca ccctcgtcgc tatgttgttc cggaggcggt
     4801 ggtggtgcct cccaggctgt ataagaatca aaatcggtcg tctgatcaat tgtaatgtct
     4861 tcggaaaggt cttcgttttc cgactcgatc gggatggatg gacgagacgg tggcttggcg
     4921 gcttcgattt tttgttcaat ttgcggaata tcagttactt ccaaaataac atcaactttt
     4981 ctctggacta cttcgcgttc ttcaaatttg gggaaagcaa tcataatccc tgtaattaat
     5041 accagagcga caaccagtcc aactcgatac atcagcggtt gacgtaattt caaacttgct
     5101 tcgggttttt ttcttaaaat catagtctaa cccttaacta gctgttttag tggaataatt
     5161 gactttaagc gcatccgcta accgcagttc tttatgaatc ttgctcatta agtccatcgt
     5221 aaccctttca tcagcccgaa tcgaaacaat taactgcgga tctctaacac gtttgttata
     5281 cataatattt ctaatgtcct ggagatttac cagcttacca tctattgaaa tcaattcctc
     5341 tttagaaacc cacaactcgg ttacatggat cttggactga agtttctcaa tttgtttggc
     5401 tttgggtaaa actaccggta aaccagaata ttgacgcatc actgtggtag ccatgaagaa
     5461 taaggttaac gtgaaaataa tatctggcat tgaggctgtt ggaacctccg tactgatatt
     5521 ttttttgtgt tgaaaagcca tttcagtact ccttattcag caatagaaat tctggtagct
     5581 ttagccattt ttaattgatc caaaacagcc acgtatttat cgtatcggca ccgcgggtga
     5641 gctttaaccg aaaaaatcat tttgggattt tccatctgta atctttcggc aatatctcga
     5701 atctgattaa tttcaacttc tttattatca attaacacgt taccctgcgg atcaattacg
     5761 attgtagtga tgttcttttt agaaatctct accgggctgt caccttcggg tgggagtatc
     5821 agtccaatgc ctttatctac atcaaccgtg gtcgtgcaaa taaagaaaag gagtaaaaga
     5881 aaggctatgt ctgcctgcga ggcagatgga atatcaactt tgcgagtttt cttttttttc
     5941 agcagagcca tatttacttg caattttcct tattaataac atcaagagtt tgcaataaat
     6001 tcatagtact ttcttccatc tcgactacaa tactgtccac gcgtgctacg aatagattat
     6061 aacataactg tagcggagct gctacaatta aaccaaataa ggttgttaat aaagcttcag
     6121 aaataccacc agctacaata gcaggggaca tatcattgac ttttgcgatg gcgtcgaaag
     6181 cattgatcat accagaaacc gtacccataa agcccagtaa cggagccagg gtaatgacgg
     6241 tcgccagcca aatcatacct ttatcaagaa atgccatttc gacggttcca gcactctgga
     6301 tcgccttttc aacggcttcc atcccatttc ctactgccga taaaccagca tggaagatct
     6361 cagctatcgg accaggagta ttttcacata actcaatcgc tttcttgact ccatcttctt
     6421 ctttcaaagt attaagaact tttggaagaa agcgtttggt tatccgcgaa gccttagaca
     6481 gtgtatatgc cctttcaatc acgaccatta taccaaatat tgctaatccc agaataggcc
     6541 acatgaaaat tccgcctcta agaaacagtt caaccattat ttatttacct ccataattac
     6601 aattctaatt tcaattttta atgctgacaa ttttaactcc ataatttaaa aaagtcaatg
     6661 atattctcaa ttaataatta ttgaatctga agcatttttc gggcttcacc caaataatca
     6721 acggcagacg tgacttgatt atccgaaagc cgcacaatct tatagtattc atcgtagccc
     6781 caataaccac gcgcgatttc ggctttaatt aaaattttca aatacttagc atctttctca
     6841 acttgcttaa tattcaaatc gccaattttt tcgaccgcca ttttcttaaa tgcctcgaaa
     6901 tctttattgc taacctcaaa atttttataa aaatttttac gatcggcggc taaatcccga
     6961 ttcttattaa caaaatcgga ggcatattca aaaataactc gcgagggatg tctcagtaat
     7021 ttgtaggtct cattattcaa cgcttgatca tatttcaccg aataatcagg tgtaatcccg
     7081 ccgccaccat aaaccgtccg accaccaata gtgctaaact tcggccgttt ctcgaccgaa
     7141 tccttgacag ctaacaagga atcacggttt tctgcactaa aagtgtcata atagtcgtca
     7201 attgccccat cgtaaggccg ctggatcagc cgcccactgg gagtatagta tctggcgaca
     7261 gttactctaa ttgccgagcc gtctctcaat ggatattgac gttggaccag gcctttgccg
     7321 aaactggttt cgcctacaat tagtccgcga tccagatcct gcaccgcacc ggcaacaatt
     7381 tcactggcgc tggctgaacc ccggttaatc agaattacca ggggtatctt ttcatagggt
     7441 ccggttttac tcgaataaaa ttcttcatta gcactcgaaa ttcgtcccct ggtataaacg
     7501 attttttggc gaccggcgat aaatttatca gcgatttcca ccgcctgttc cagataaccg
     7561 ccgctattat tacgtaaatc aaaaaccaga cgttgcatgc cttccgcagc caatcggttt
     7621 aaagcctttt cgacttcgtc actggtcgtt ccgctgaacc gatttaagaa aatgtatccc
     7681 gtactatcat ctatcataaa agccgccgaa acgctataaa gcggaatttc atcccggata
     7741 atcttgacat caaaatcggt ttgcccctgt cgacgaaccg taacggttac ttccgagcct
     7801 ttgggaccac gcagtttttt ctggacatca tcgcgactaa tacgataggc ggattcacca
     7861 tcaattttga cgattttatc gccgacttcc agtccggctc tttcagccgg agctccggca
     7921 accggtgata tgaccgttat ataaccgtcc aaaatatcga attcgatacc gattccctca
     7981 aatttaccgg aaaattgctc atttagatca tccaattgtt tgggctcgat gtaaaccgaa
     8041 tggggatcta attcttccag cattccccgg taagcgcctt ccattacttt attccaatcc
     8101 ggttcttcca catagctgtc atttatgatt cgaatgatat cctgaaaaat gtttaatttt
     8161 tggtataagt cactccccga ggcaatgatt gacttgccaa acggggaaat aaataaaaat
     8221 cctaacacaa cgataattat tataaccgga atggttgcct tgcgcataat ttgtctcaat
     8281 ctaacatagt taattctttt ataaatcatt taaatagcaa attaaaaatc aaaccttaat
     8341 ttaacctttt attagcataa ttgttgccta tatctaaaaa ataactgata taatttgccc
     8401 cacttgttcc agaaattgtc tttcggaatt accgaaccga tccgattgat gagaatcaat
     8461 gtctaattca gctacgaact gaccggattt aaaaataggg ataacgattt ctgaccgaac
     8521 ctcaagacta caagccagat aattcgattc agccgtaaca tcagccacat tgaccgttgt
     8581 gcccgaaacc gccgcacgtc cgcaaactcc gcgaccaaat gggatattcc tgtgttcggt
     8641 gggatctccg ataaacggtc ccaaaattag ctcctttttg agattatcac tgaagtaaaa
     8701 tccaacccaa tcaaagccga taaatttatc attgagaaag cggcaaattt ccagtagttt
     8761 agtgtcgcga ctaccacgac ggttaactat ttgcttgatg tactcgagtc cctctcgcat
     8821 gaacttatct ttattctccg gcgatttcaa gatttttctc tccttataca gctcgcacta
     8881 gatcgaatta aaaaatgaca ggctgaactt aatctttgcc caggtttttt aggactcctt
     8941 gcgccgcaaa aataccggtc accgccgaat agacaatgcc ccgactatgt ccgctggcat
     9001 caccagccac gaaaaaattt tcaagtgtag tttccaaata gggcgttact ttatatttgg
     9061 tatcgtaaaa ttttatttcc ggggcatata gcaaagtatt attggacatc agcccggaaa
     9121 taattttgtt taatacggta agcccttcga tcaaattggt cacaatgcgg tgaggaaaag
     9181 ccatcgcaat atcaccgggc gtgacatcct tcaaagtcgg taccgtagcg gagcggctaa
     9241 tgcgttccca atttgagcgt ctgcccgaaa caaagtcggt cagccgctgg ataatgggct
     9301 taccgccgcc gattgtggta gccagacgag caatatcgcg tccgtacttg gtcgtatctt
     9361 caaccgggtc ggtcaagacg actcgcgaaa gtaatgcaaa attggtgtta tcggttttgg
     9421 aattccattt ggcatgaccg ttaaccaaca caaaatcgtc atatttttcc gtaacgatat
     9481 agccactcgg attcgtgcag aaggtgcgta ccagatcatc ataagattta ctataaagtc
     9541 gaaacttagc gtcatacata acggcggcaa ttgggcgata aatatcatct ggaaattcaa
     9601 cccgcactcc aacatcaatc ggtccataca tcgtgtgaat cccgagatta tg
//
LOCUS       DAOMJU010000083         9540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_7458_length_9540_cov_14.025619, whole genome shotgun sequence.
ACCESSION   DAOMJU010000083 DAOMJU010000000
VERSION     DAOMJU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9540
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7458_length_9540_cov_14.025619"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /gene="araA"
                     /locus_tag="PLO58_07850"
     CDS             complement(<1..1373)
                     /gene="araA"
                     /locus_tag="PLO58_07850"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HQO74740.1"
                     /translation="MKVFENYEVWFVTGSQHLYGEDVLRQVADHSREMAKYLSDSPNI
                     PAKVIFKSVLTEPEAILHLALEANSAKNCIGLIVWMHTFSPAKMWIAGLKNLQKPFVH
                     LHTQYNRDLPWSEIDMDFMNLNQSAHGDREFGFICSRMKINRKVIVGHWRDENVINEL
                     AIWVRAAVAWQDSRTGKIARFGDNMREVAVTEGDKVEAQIRFGYSVNGYGIGDLAQVV
                     YSIPEADIDKLIEEYNAKYIVAKEAAKGGEQYDALREAARIELGLRQFLKCGNFTAFT
                     TTFEDLHGLKQLPGLAVQRLMADGYGFGAEGDWKTAALVRAMKIMATGLPGGTSFMED
                     YTYHFNPSGMKVLGAHMLEVCESVAANKPRLEVHPLSIGGKADPPRLVFNAPTGPALN
                     VSIIDLGNRFRMILNEVESVQPDADLPKLPVARVVWMPKPNLKTAAQCWILAGGAHHT
                     GFSQALTA"
     assembly_gap    1464..1563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1564..1928)
                     /locus_tag="PLO58_07855"
     CDS             complement(<1564..1928)
                     /locus_tag="PLO58_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQO74741.1"
                     /translation="MLEELKAEVLNANRALVQYGLVILTWGNVSGIDRAKNLVVIKPS
                     GVEYDKLTIDDLVVVNLDGRVVEGNRRPSSDTPTHLALYRAFDNIGGISHTHSDYATT
                     FAQAGCAIHCYGTTHADYF"
     gene            complement(1913..3637)
                     /locus_tag="PLO58_07860"
     CDS             complement(1913..3637)
                     /locus_tag="PLO58_07860"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HQO74742.1"
                     /translation="MVKTKKYTIGLDFGTNSCRSLIVDISNGRELATHVFNYPSGEAG
                     IIVDSANPNLARQNPADYLLGLEVTIREALRKASQIEPDFSPKDVIGIGVDTTGSSPM
                     PVDQEGLPLCFQAEFKANPSAMVWLWKDHTSYAEAQKITELARQLRPQYLAKIGGVYS
                     SEWFWSKLWHLQSECPEVFQNTFSFVEICDWIPAVLTGNTDPLLIKRSICAAGHKALF
                     NEQWGGLPDEEFLSELSPEIAAMRQRLYRKAYSAEEKMGNLSPEWAEKLGLTTEVAVA
                     VGAFDAHMGAVGAGIATGILVKILGTSTCDIMVWPNSEKLNDIPGVCGIVDGSVLKGY
                     FGIEAGQSAVGDIFLWFVNNLVPDSFGKTQEEKFENLAKAAGGLKPGESGLLALDWNN
                     GNRTILVDVRLTGLLVGQTLHTQAHEIYRALIEATAYGALAIIDRIESYGVPVREIVN
                     CGGLAVKNPLLMQIYADVTGRPMKVSTSEQTPALGAAIFAALAGGKAHGGYDNITEAQ
                     RAMTSTGKEYLPDNRNHQTYKELYTLYKQMHDAFGTPEWSGKMFNVMKDLLNIRDRVR
                     QEKLCSKN"
     gene            complement(3671..>4191)
                     /locus_tag="PLO58_07865"
     CDS             complement(3671..>4191)
                     /locus_tag="PLO58_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/glucose cotransporter"
                     /protein_id="HQO74743.1"
                     /translation="VATAVMVLIGLIWIPVIRGGKGLYDYLQGVQSYLAPPIFVVFFL
                     GVFWKRANAKGCLATLITGFTLGLFRLAVDTPVKLLNVKYAEGSFLWIVNNIYFQYYS
                     LLITIISILVMIFVSLGTKEPDYEKIKGLTYGTRTEEDRKELRANRSRGDVIASVILV
                     VLIVLCYIYFSG"
     assembly_gap    4192..4291
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4292..5534)
                     /locus_tag="PLO58_07870"
     CDS             complement(<4292..5534)
                     /locus_tag="PLO58_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096059.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HQO74744.1"
                     /translation="MLQTLDWIIIVGYFGIILGIAWWVMRQKQETSTDYFLASRNLGW
                     FIIGASIFASNIGSEHLVGLAGSGATDGVAMAHYELHAWCLLILGWVMIPFYMRSKVF
                     TMPEFLEKRFSPRARTVLSLISLVAYVLTKIAVGIFAGGIVFSVLLPDLNFMGLDSFW
                     VGSVLVVVLTGLYTILGGLKAVAYTEALQTIVLVFGSILVTLFGLKALGGWGELREIA
                     GSEMFNLWKPLIPHGVEGTWAPIKESGRMAWYFNDNYPWIGMLLCAPIIGLWYWTTDQ
                     YIVQRALAARDIKTARRGTIAAGFFKLLPVFIFIIPGIIAYALAVSGKIPALQSQLIG
                     PGGEVIRDNSQSAFPLLVSYVLPVGIRGIVVAGLLAALMSSLAGVFNASATLFTIDFY
                     SRFHPEASQKKLVWIGRVATAV"
     gene            complement(5562..6689)
                     /locus_tag="PLO58_07875"
     CDS             complement(5562..6689)
                     /locus_tag="PLO58_07875"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HQO74745.1"
                     /translation="MDKKLLIAITALTSILTINCAKKEQGSQASVEIFGTLSNGTEIK
                     KYTISNSKGLTAEIINYGARVVSLKAPDRTGKIEDIIFGFDDLASYERDNSYQGAIVG
                     RYGNRIRDGKFTLEGKTYQLDINDGNNHLHGGKDGFYRQVWECRAADSRSIQLRYISP
                     DGEGGYPGQVEITVIYTITDDNGLKIEYSAKTDKTTILNPTSHCYFNLSGFPEKSILD
                     HELMINADYFTPVDAGLIPTGEIRPVVGTPMDFRTVKAIGKDIEADDEQILYGNGYDH
                     NWVLNDYNGQVRLSATLYEKETGRLMEVLTDQPGLQFYSGNFLDGSMTGKGGVKYNHR
                     TALCLEAQAFPDSPNKPNFPSVVLRPGEEYKQTTIYRFSTK"
     gene            complement(6903..>9540)
                     /locus_tag="PLO58_07880"
     CDS             complement(6903..>9540)
                     /locus_tag="PLO58_07880"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQO74746.1"
                     /translation="HTNFKISASGESIILSDTSGTIIDQVDLPESMVDISYARITDGS
                     PDWVFQLPTPGSANTGTETLGTSDEVTVSYPGGFYSTAISVELTAGDCRIFYTLDGSD
                     PDSNKTEYLSPINIDSTAVLKAISYKANYLPSPILYQTYFINESTSLPVISLSTDPYN
                     LFDYNYGIYANGPGYTSADPHYGANFWMDWERPAHVEFYDDDKQLGFSKNCIIAIYGA
                     WSRAHPQKSFSVKFKDGIEPLEYKLFPDLDLTTYKAFVLRNSGNDFYNTHMRDAMMQT
                     LIQDLDIDYLEYRPAITFINGQYWGIYNIREKINEHYVAYHHGVDKDNIDMLENNMEV
                     IHGDTENYQQIINYIDTHDMSTDAAYNFIDSTIDLNECLLYFAAQAYYNNRDWPGNNQ
                     KYWRARSGSKTKWRWIIYDLDFGFNLYESDGHKEDHISFMFTVNNSNYAQAEWAIKLQ
                     KKLIENPTIRNRFINQIADLLNTNFKSERVVNTINEMADHIADELPRHRQRFGIGGEN
                     RDKMITFAKQRPAYLRNHVRNYFKCGVDGNLTIDITEGGTVKLNTLSLQAADAPLTGI
                     YFQGNAVNLEAIPKPGYKFDGWSGAVTSSDVSLSINVTNSTNLVATFSAANDVVKDVV
                     INEINYNSADDFDSGDWVEFYNNSDSSIDISDWFFKDNDDSHIFTFPSGTILAPSAYL
                     VLGEESEKFIACYPDVTNFVGEMDFGLSGSGELIRLFDSEGQIVDSLTYSDKAPWPAE
                     ADGQGATLELFAPNLDNTNGENWRASVGHGSPGQQNTVTVAIAPNDSREIPEKFNLMQ
                     NYPNPFNHSTVILFQISEPSLVKLSVYDLNGRLIEILVNEYRNPGYYAVHWNAENVVS
                     GIYFYRIEAGSLSSVKKCLLIK"
BASE COUNT         2678 a         2198 c         1837 g         2627 t
ORIGIN      
        1 gcggtcaagg cttgactgaa gccggtatga tgagctccac ctgccagaat ccaacattga
       61 gcggcagttt tcaggttagg tttaggcatc caaactaccc gagcaactgg taattttggc
      121 aaatcagcat ccggttggac gctctcgact tcgttgagaa tcatccggaa acgattacca
      181 aggtcaatga tggaaacatt gagcgctgga ccggtcggag cattgaatac caaacgcggc
      241 gggtcagctt tgccaccgat tgagagtgga tgaacttcta agcggggttt attggcggcg
      301 acagattcac atacttccag catatgtgcg cctaagactt tcattcctga tggattgaaa
      361 tgataagtat aatcttccat aaacgaggtg ccaccgggta agccggttgc cataattttc
      421 atcgctcgta ctaatgcggc agttttccaa tcgccttccg ctccaaaacc gtaaccgtcc
      481 gccattaagc gctggactgc caaaccggga agttgcttca aaccgtgcaa atcctcgaag
      541 gttgtcgtaa atgcggtaaa attgccgcat tttaggaatt gtcgcaaacc cagttcaatt
      601 cgggcggctt ctcgcagagc gtcgtattgc tcgccacctt tagcggcttc cttggcaacg
      661 atatatttag cattgtattc ttcaatcaat ttatcaatat cggcttcagg aatactgtaa
      721 actacttgtg ccaaatcgcc tattccataa ccatttaccg aatatccgaa ccggatttga
      781 gcttcgactt tgtcgccttc tgtgacggca acctcgcgca tattatcgcc aaagcgcgcg
      841 attttcccgg tgcggctatc ttgccaggcg accgcggcgc ggacccagat tgccaattca
      901 tttatgacgt tttcatcgcg ccagtgcccg acaatcacct tgcggttgat tttcattcgg
      961 gaacaaatga agccgaattc gcgatcgccg tgggctgatt gattcagatt cataaaatcc
     1021 atatcaattt cggaccaggg cagatcgcgg ttatactgag tatgcagatg aacaaatggt
     1081 ttttgtaggt ttttcaatcc ggcgatccac atttttgccg gtgaaaaagt atgcatccag
     1141 actatcaaac cgatacagtt tttggcgctg ttggcttcca acgcaagatg caaaatcgcc
     1201 tctggttcag tcaaaactga tttaaaaatt actttggcag gaatattcgg cgaatcagat
     1261 aggtacttag ccatttcgcg ggaatggtcg gctacctggc gcaaaacatc ttctccgtac
     1321 aggtgctgac tgccggtaac aaaccaaact tcgtaatttt caaatacttt cattttttat
     1381 tcctttattg attatttatc attttgaccg taataggctg atggtccatg tttccgtaag
     1441 taatgctttt gcaggatata ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnttgaaat aatcggcgtg cgtcgtgcca tagcaatgaa ttgcgcaacc ggcttgagca
     1621 aaggttgtag cgtaatcgct atgagtgtgg ctgattccgc caatgttgtc aaacgctcga
     1681 tataatgcca agtgtgtcgg agtatccgat gaagggcggc gattgccttc aactactctc
     1741 ccatccaaat tcactactac cagatcgtca atcgtcaact tatcatattc aacgccactc
     1801 ggtttgataa caaccaggtt tttcgcacga tcaatgccac tgacattacc ccaggttaaa
     1861 atcaccaaac cgtattgtac caatgctcgg ttggcattca gaacttctgc ctttaattct
     1921 tcgagcacaa tttctcctgc cttacccggt cgcgaatatt taacaaatct ttcataacgt
     1981 tgaacatttt tccagaccat tccggagtac caaaagcatc atgcatctgt ttataaagag
     2041 tatataactc tttgtatgtc tggtgattac ggttatcggg taaatactcc ttgccggtgc
     2101 tggtcatcgc tcgctgagcc tcggtaatat tatcatatcc accgtgcgct ttgccaccag
     2161 caagcgccgc aaaaatcgct gcgcccagcg ccggagtttg ttcactagta gaaactttca
     2221 tcggtcggcc ggtgacatcg gcatatattt gcatcaatag tggatttttg actgccaaac
     2281 caccgcagtt gacaatttct cgtacgggaa cgccgtagct ttcaattcgg tcaataattg
     2341 ccaaagcgcc ataagccgtc gcttcaatta atgctcgata aatttcgtgt gcttgagtat
     2401 gcaaagtttg accaaccaat aaaccagtca accgaacatc aaccaaaata gtgcgattgc
     2461 cattattcca gtcgagtgcc agcaaacctg attcgccggg ttttagacca ccggcggctt
     2521 tcgccagatt ttcgaacttt tcttcttgag ttttaccgaa ggaatcaggc actagattat
     2581 taacaaacca cagaaaaata tcgcccaccg ctgattgccc ggcttcgata ccgaaatagc
     2641 ctttcagaac tgaaccgtcc actatcccgc atactcccgg aatgtcattc aatttctcgc
     2701 tattcggcca gaccattata tcgcaagtgc tggttcccag aattttcacc agaatcccgg
     2761 tcgcaattcc agctccaact gctcccatat gggcatcaaa agctccaacg gcaacagcca
     2821 cttcagttgt caatcccaat ttttccgccc attctgggga tagatttccc atcttttctt
     2881 cggccgaata ggctttccgg taaaggcgct gacgcattgc ggctatttcc ggagatagtt
     2941 cactcagaaa ttcttcatca ggcaaaccgc cccactgctc gttgaacagg gctttatgcc
     3001 cagcggcgca gatgcttctc ttaattagta atgggtcggt attacccgtc aatacagccg
     3061 gaatccagtc gcatatctcg acaaaactaa aagtattctg aaaaacttct ggacattcgc
     3121 tttgcagatg ccagagtttg ctccaaaacc attctgacga gtacacacca ccgatttttg
     3181 ccaaatattg tggccgtaat tgacgagcaa gttcggttat tttttgagct tcggcataac
     3241 tggtatgatc tttccagagc cagaccatag ccgaagggtt ggctttaaat tcagcttgaa
     3301 aacagagcgg cagaccttct tgatctacgg gcattggact acttccggta gtatctactc
     3361 cgattccaat tacgtcctta ggtgaaaaat cgggttcaat ttgggaggct ttacgaagcg
     3421 cctctcgaat tgtaacctct aagcccagca gataatcagc cgggttctgg cgagcaaggt
     3481 taggatttgc cgaatcaaca ataatcccgg cttcacccga aggataatta aaaacatgtg
     3541 tcgctaattc ccggccgtta gaaatatcta caatcagcga acggcaagag ttagtgccaa
     3601 aatctaatcc gatagtatat ttttttgtct taaccataat atccaaaaaa taatgttaac
     3661 tgcttgaata tcaacccgaa aaatagatat agcacaaaac aatcagaact actaatatga
     3721 ccgaagcgat tacatcgcca cgtgaccgat tagcacgtaa ttccttgcga tcctcttcgg
     3781 ttctggtacc ataggtcaaa cctttgatct tttcataatc aggttctttt gtgcccaaac
     3841 taacaaaaat cataacgaga attgatatta tcgttatcaa aaggctgtag tattggaagt
     3901 agatgttatt cacaatccac aggaaagaac cttcggcata tttaacattt aacaatttta
     3961 ccggtgtatc aactgccaat cggaaaagac caagggtaaa gccggttatc aaggtagcca
     4021 gacagccctt tgcattcgcc cgtttccaaa aaactcctaa gaaaaagacc acaaaaatag
     4081 gcggtgctaa ataagattga acaccttgta aataatcata aagtcccttt ccaccccgaa
     4141 ttaccggaat ccatatcaga ccaatcaata ccataacggc agtagctacc cnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntaacggcag tagctaccct gccaatccaa
     4321 accagcttct tctgactagc ttccgggtgg aagcgcgagt aaaaatcaat cgtaaaaagg
     4381 gtcgccgaag cattgaaaac gcctgctaag gaactcatta acgccgctaa taatccggca
     4441 acgactatac ctcgaatccc gaccggcaag acgtaagaca ctaagagtgg aaaagcgctc
     4501 tgagaattat ccctgataac ctcacctccc ggtccaatta gctggctctg aagggctgga
     4561 atttttccgc taacagccaa agcgtaggca attataccag gtattatgaa aataaaaact
     4621 ggtaataatt taaaaaatcc agcggcaatg gtgccacgcc gcgctgtttt tatatcccga
     4681 gccgctaatg ctctctgcac aatatactgg tcggtcgtcc aataccatag tccaataatt
     4741 ggagcgcata acagcattcc tatccaggga taattatcat tgaaatacca agccatccgg
     4801 ccgctttctt tgattggcgc ccaggtgccc tcgacgccat ggggaatgag tggcttccag
     4861 aggttaaaca tttccgaacc ggcgatttcc cgcaattctc cccaaccgcc gagagccttt
     4921 agaccgaaca gagttaccaa aattgaaccg aaaaccaaaa caatcgtctg taaggcttcg
     4981 gtataagcaa cggctttcaa accgcccaat atcgtatata atccggtcaa caccactacc
     5041 agtaccgaac caacccagaa actatctaat cccataaaat tcaaatccgg caatagcaca
     5101 ctaaatacaa taccaccggc aaaaattcct acggcaattt tagtcagcac ataagccact
     5161 aacgaaatta gagacaaaac tgtccgggct ctgggcgaga aacgtttttc caaaaattcc
     5221 ggcatcgtga atactttcga acgcatatag aaaggtatca tcacccaccc aaggattaat
     5281 aagcaccagg catgtaattc gtaatgcgcc atagcaacgc cgtcggtcgc tcccgaaccg
     5341 gctagtccga ctaaatgctc cgaaccgata ttcgaagcga aaattgaagc tccgatgatg
     5401 aaccagccca aattgcggct agcaagaaag taatctgtgg atgtttcttg cttctgcctc
     5461 attacccacc aagctattcc gagaatgatt ccaaaatagc ctacaatgat tatccagtct
     5521 aaagtctgta acatagctat ttccccttgt taaaagttga actattttgt cgaaaaacga
     5581 tagattgtcg tctgcttata ttcttcaccg ggtcttaaaa ccaccgacgg aaaattaggc
     5641 ttattgggcg aatccggaaa agcctgagct tccagacaaa gagcggtacg gtgattatac
     5701 tttacccctc ccttgccggt cattgaacca tccaggaaat ttccagaata aaattgcaaa
     5761 cccggttggt cggttaaaac ttccatcaat cggccagttt ctttttcgta caaagtcgcc
     5821 gaaagtcgaa cctgaccgtt atagtcattc agaacccagt tatgatcgta gccgttgcca
     5881 tataaaattt gttcatcatc ggcttcaatg tctttgccga tcgcttttac cgttcgaaaa
     5941 tccatcggtg tacctacaac aggccgaatc tcaccggttg gaattaaacc ggcatccacc
     6001 ggcgtaaaat agtcggcatt gatcattagt tcgtgatcta agatactttt ttccggaaat
     6061 ccactcaaat tgaaatagca atgactggtt ggattcaaaa tcgtcgtctt gtcggttttg
     6121 gcactatatt cgatcttgag tccattatca tccgtaattg tataaataac agttatttca
     6181 acttgtcccg gatatccgcc ttccccatcc ggactgatgt aacgtagctg aattgatctg
     6241 gaatcagccg cgcgacattc ccaaacctgt ctataaaatc cgtcttttcc accgtgtaga
     6301 tgattattac catcattaat atcaagttga taagtctttc cctcaagagt aaacttacca
     6361 tcccgaatgc ggttaccgta gcgcccaacg atagcgcctt gataggaatt atcgcgctca
     6421 taagaagcca aatcatcaaa accaaagata atgtcttcaa ttttgccggt acgatcaggc
     6481 gctttcaatg atactactct ggctccgtaa ttgataattt cggctgtcaa tcccttacta
     6541 ttggaaatcg tatatttttt tatttcggtg ccatttgaca atgtcccgaa gatttctacc
     6601 gaagcttggc taccctgctc ctttttcgca caattaatcg tcaaaatgct ggttagagcg
     6661 gtaatggcaa tcaataactt tttgtccatc tttattactc tccttatctt tctcaattca
     6721 aattccattt tgagttcaat tataataaag aaattgatta aaaaaaagac gttttataaa
     6781 taaaaccact aagtcactaa ggcacgaaga atttatttac ttttaaacta cttaagcatt
     6841 ataaagacaa tcagattaaa taattttaaa caatgcgaaa gccttccaaa gatcatacga
     6901 ttctatttaa tcaataaaca tttctttaca ctgcttagac taccagcctc aattcggtaa
     6961 aaatatattc ctgagacgac gttttcagca ttccaatgaa ctgcataata accgggattt
     7021 cggtattcat tcaccaatat ttcgattaat cttccgttga gatcgtaaac tgataatttg
     7081 accaacgacg gctctgatat ttgaaataat atgacggtgg agtggttgaa cggattgggg
     7141 taattttgca tcaaattgaa tttttcaggg atttcccgac tatcatttgg agcaatcgca
     7201 actgttacag tattttgttg accaggagaa ccgtgtccga cggaagcccg ccaattttca
     7261 ccattagtat tatccaaatt cggagcgaat agctctaaag ttgctccctg tccatcagct
     7321 tcggctggcc agggagcttt atcgctatag gtgagtgaat caacaatctg accttctgaa
     7381 tcaaacaacc ggattagttc accgcttccg cttaatccga aatccatttc accgacgaaa
     7441 ttggtcacat ccggataaca agcaataaac ttttcgctct cttcgcctaa aacaaggtag
     7501 gcagacggtg cgagaattgt tccgctcgga aaagtaaata tgtggctgtc gtcattatct
     7561 ttgaaaaacc aatcgcttat atcaatcgaa gaatcactat tattataaaa ctcgacccaa
     7621 tcacccgaat caaaatcatc ggcagaattg taattaattt cattaatgac gacgtcttta
     7681 actacatcat tagcagctga aaaagttgca accaggttcg tggaattagt gacattgatg
     7741 cttaatgata catcggaact ggtaactgct ccactccagc catcgaattt atatccgggt
     7801 tttggtatag cttcaaggtt caccgcgttt ccttgaaaat agattccggt caagggagca
     7861 tctgcggctt ggagtgataa agtgttgagt tttacggtgc cgccttcagt tatatcaatt
     7921 gtcagatttc catccacacc acacttgaaa tagttccgca cgtgattgcg tagatacgcc
     7981 ggtctctgtt tggcaaaagt tatcatctta tcccgatttt ctccaccaat tccaaatcgt
     8041 tgtcggtgtc tgggaagttc atcggcaata tgatctgcca tctcattgat ggtattgaca
     8101 acccgttcgc ttttaaaatt ggtattgagc aaatcggcga tttgattgat gaatcggttc
     8161 cggatcgtcg gattctcaat taatttcttt tgtaatttta ttgcccattc ggcttgagca
     8221 taattactat tattaaccgt aaacataaaa gatatatgat cttccttatg accgtccgat
     8281 tcataaagat taaaaccgaa atccaaatcg taaataatcc agcgccattt agttttagaa
     8341 cccgacctgg cgcgccagta tttctgatta ttgcctggcc aatcgcgatt attataatag
     8401 gcttgagcgg caaaatataa aaggcactcg ttcagatcaa tcgtgctgtc aataaagtta
     8461 taagccgcat cggtgctcat atcatgagta tcgatataat ttataatctg ttggtaattt
     8521 tcggtatcgc cgtgaattac ttccatatta ttttcaagca tatcaatatt gtctttatct
     8581 acgccatgat ggtaagcaac ataatgttca ttgattttct cgcgaatatt gtatatgccc
     8641 cagtattgac cgttgatgaa ggttattgcg ggacgatatt cgagatagtc aatatcgagg
     8701 tcttggatca gggtttgcat catcgcatcc cgcatatgag tattataaaa atcgtttcct
     8761 gaatttctga gcacaaaggc tttataggtc gtcaggtcta aatcaggaaa cagtttatat
     8821 tcgagcggct cgataccatc tttaaatttt accgaaaaag atttttgagg gtgtgctcta
     8881 ctccaggcgc catatatggc aatgatacag tttttcgaga agcccaattg tttatcatca
     8941 tcgtaaaatt caacatgcgc cggtcgttcc caatccatcc aaaagtttgc cccgtaatgc
     9001 gggtcggctg acgtatagcc tggaccattt gcgtaaattc catagttgta atcaaataag
     9061 ttatagggat cagtcgagag tgaaatgact ggcaaactcg tactttcatt gataaaataa
     9121 gtctggtaca atatcggcga tggcaaataa ttggctttat agctgatcgc ttttaaaacc
     9181 gcggtactgt caatattaat cggactcaaa tattctgtct tgttggaatc agggtcgctt
     9241 ccatctaagg tatagaatat ccggcaatca ccagctgtaa gttcgaccga aatcgctgtt
     9301 gaatagaatc cacccgggta agaaacggta acctcatcag aagttcctaa ggtttcagta
     9361 ccggtatttg ccgaaccggg agtcggaagt tgaaacaccc aatctggaga accgtccgta
     9421 attcgggcgt atgaaatgtc aaccattgat tcgggcaaat ctacttgatc aatgatcgtt
     9481 ccgcttgtat cactcaaaat tatgctttcg cctgaggcgc ttattttgaa attggtatgc
//
LOCUS       DAOMJU010000084         9292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_7757_length_9292_cov_17.856122, whole genome shotgun sequence.
ACCESSION   DAOMJU010000084 DAOMJU010000000
VERSION     DAOMJU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7757_length_9292_cov_17.856122"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /gene="ribH"
                     /locus_tag="PLO58_07885"
     CDS             complement(<1..305)
                     /gene="ribH"
                     /locus_tag="PLO58_07885"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQO74747.1"
                     /translation="MKLHEGVLSAEKMKVGIVASRFNDMITKELLAGALDCLKRHGVD
                     ENDIHVYWVPGALEIPRIARELADSGQFDGVVCLGAVIRGATPHFDIVANEASKGIAR
                     "
     gene            complement(301..1521)
                     /locus_tag="PLO58_07890"
     CDS             complement(301..1521)
                     /locus_tag="PLO58_07890"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HQO74748.1"
                     /translation="MKFDSIDAAVEDLKKGRFVIVVDDENRENEGDFIMAAEKVTPES
                     VNFMAKYARGLLCVGLTEERAKELNLELMVQDNTALHNTRFTVSVDLKEGTTTGISAN
                     DRALTINALANPNTKPEDLGRPGHIFPIIARPGGVLRRAGHTEASVDLMRLAGLTPVS
                     VMCEIMDENGEMARGEALEKIAQTHNVKIITVQSLIEYRRRTERFIERVSEADLPTKF
                     GVFRIIIYVDRLTDKEHVALVMGDIKSDEPVIVRVHSECLTGDVFSSERCDCGDQLHK
                     ALEIIAQNKRGVLLYLRQEGRGIGLRHKISAYKLQDEGMDTVEANIKLGFLPDLRDYG
                     MGAQILADLGVHKMRLLTNNPKKIVGLQGFNLEVVERIPIEIPPNPQNEKYLKTKRDK
                     MGHLILNNKKVPND"
     gene            complement(1548..2189)
                     /locus_tag="PLO58_07895"
     CDS             complement(1548..2189)
                     /locus_tag="PLO58_07895"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQO74749.1"
                     /translation="MFTGIIEEMGEIRAVSENVAGRRFQIAANRVLDGINIGDSIAVD
                     GVCLTVEKVGKDYIEVQAVTETLARTTLAKVPRGAKVNLERAMAASGRFGGHFVQGHI
                     DSVAPIVAFQRIGNAGTMRIAVPDSIRHYIVEKGSLAVNGVSLTVAGIENEVIEIALI
                     PVTLTETNLGEKKIGDEVNLEVDILAKYIEKILEHFEKETLTFEKIKQWGFNQ"
     gene            complement(2189..3316)
                     /gene="ribD"
                     /locus_tag="PLO58_07900"
     CDS             complement(2189..3316)
                     /gene="ribD"
                     /locus_tag="PLO58_07900"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HQO74750.1"
                     /translation="MREDEKSRAQSGFFMNQNEKWLKRALELAEKGRGFTSPNPVVGA
                     VIVSGEKVVGEGFHSKFGAPHAEIKALEQAGESARGAILYVNLEPCCFYGKTPPCTEA
                     IIKSGISEVYIGTIDPNPNVNGAGVKSLEQAGLKVQVGILEREARSANRGFFTFIQKK
                     RPWITLKMALTLDGFIADATGKSRWITGVEAREFVHKQRQQYDAVVVGMGTVYKDDPE
                     LLPENRSGYIPYRVVLDEALNLPPRMKLVTDGFQRRTIIVTACQEKAEKIRQFESSGI
                     KIIQSSKDEFGWLDLPKTLAKLAEFGVTSIFCEGGSQVAGSLLNQRLVDELQVFIAPK
                     ILGEGLRAFSGFMQSLDQALMVEWEKVERLGADVLLQGKLV"
     regulatory      complement(3301..3424)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3479..4717)
                     /locus_tag="PLO58_07905"
     CDS             complement(3479..4717)
                     /locus_tag="PLO58_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HQO74751.1"
                     /translation="MKLKAQLVEYFKSNQADVRSQIIALVTEMVRQKTINVVTEKLPE
                     FPFLKMRGEEYRIAEIVQRELDKAGIPYEIFARAEGRPNVISRLGQNKSGQRLLMPAH
                     MDVVPAGEGWLTDPFEVVEKEGKLYGRGTLDNKGPLASILIAAQILKKLGIDQQLTGE
                     LMVAALSDEEAEDPDGINYGIGYLVEENLINPTMAIVPDIGGNMLEIDVAEKGRTVIK
                     ITAIGKQAHGSTPEKGINAIFMMAKLVDELERLKFKYEVHPVLGNPTLNLGEIHGGVA
                     SNIVPGSCYIYLDIRSVPGQTEEILLEQIQECIAKVPNGNFKVEIISTNQPHAISPDV
                     ELVRKIQANAREYLNIEPQAIGQGGGTYAKTLCLNGIAAVGWGPGDDNAFHVANEYVE
                     IKQLVDFALMTCLLAVDLLV"
     gene            complement(4833..5732)
                     /locus_tag="PLO58_07910"
     CDS             complement(4833..5732)
                     /locus_tag="PLO58_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQO74752.1"
                     /translation="MSNAYLGELAALLVAICWSFSSLSFEAAGKRIGSLQVNFIRLVL
                     AFIFLTLFNGIFRHQFWPRSFSAHAWFWLSLSGLVGFVFGDYFLFNAYVIVGARVSML
                     VMSLVPLITAVIGWLFLGEVMNLREILAMLVTICGVAIVIVGRNTENGKLKFSQPATG
                     ILYALGGAIGQAVGLVLSKYGMSDYNAFGATQIRVITGMIGFAVIFLILKRFPLLAKV
                     VHNRDAMKYTTIGAFIGPFLGVSLSLYAVQRTITGIASTIMAIVPVLIIPLEVLIKKE
                     KITFREVAGAIIAVGGVALYFIE"
     gene            complement(5809..8091)
                     /locus_tag="PLO58_07915"
     CDS             complement(5809..8091)
                     /locus_tag="PLO58_07915"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQO74753.1"
                     /translation="MNLNYLFLIAPIGSIIALVTAWIFYKLMLKNDPGNDQMQKIALA
                     VREGAFAYLKQQYKVVALFFLIIFVLFLIMSFGLNVQNKWVPFSFLTGGFFSALAGYF
                     GMSTATLASNRTAQGARKSLNAGLQIAFRSGAVMGLVVVGLGLLDISMWFAILYWLVP
                     SLGFDQMSLAAITTTMLTFGMGASLQALFARVGGGIFTKAADVGADLVGKVEAGIPED
                     DPRNPATIADNVGDNVGDVAGMGADLYESYCGSILATAALGVAAFQGQPHQKILAALL
                     APMIVAGVGNIFSVIGAFAVRTEEKADQRTLLKALSRGVNLSVVLIAIFTAILMYFLK
                     LPMGIVASVITGLLVGWTIGKITEYYTSQSYKPTRWIAEQSKTGPATVIIAGLSTGML
                     STGLPVLTAGIGIILSYAFAGGFGADPNHINLGLYGISFAAVGMLSTLGITLATDAYG
                     PIADNAGGNAEMSGLPKEVRQRTDALDSLGNTTAATGKGFAIGSAALTAMALIAAYIE
                     EIKAQFSHIINNPAILQNLLSEVNIQFDPGASLKTLQELVANNLHLPQWIEYYNVTLM
                     NPRVLVGMFLGSMVTFVFAALAMKAVGRAAGNMVEEVRRQFREKPGIMEGKDEPDYAS
                     CVAISTKGAQREMILPSLLVISVPVLTGLILGVAGVLGVLAGGLTTGFVLAVMLNNSG
                     GAWDNAKKYIEEGNLGGKGSDAHKAAVVGDTVGDPCKDTAGPCLNIVIKLMSTVSIVT
                     VGLAVKFGPVIGTWLGIGPR"
     gene            8361..>9292
                     /locus_tag="PLO58_07920"
     CDS             8361..>9292
                     /locus_tag="PLO58_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74754.1"
                     /translation="MKRFGLSYKLILASIVVLFMITLTWAEKPTLVIMDFEANGIPPE
                     EAKVFTEHLKVELFNSENYSIIEKSGINQKLQEEGFSGDKCATVDCLVQAGKFCGAEQ
                     AVGGSITKIGDTYSVMVRLVDVNHSQLLKTFAYDHKGDIISLLSDGTSKVAAKISEPK
                     KLNAEPVVAEKVGTVVEDTVIIAPARGKGGIVPALASCMIGPRVGLEMNEGNDTIYQS
                     EWIALGGSVVGSALTGYFAPLGSAISVGTRAYMAYDMGGKNNGLEGALASYCLGPRVG
                     NELHYRKIRTLEWLQLVPCVCIYPMIALPLEAYKG"
BASE COUNT         2656 a         2352 c         1830 g         2454 t
ORIGIN      
        1 cgagcgattc ctttggaagc ctcattggca acgatatcaa aatgaggtgt cgcgccacga
       61 ataacggctc ctagacaaac aacaccgtca aactggccag aatcagctaa ttcgcgagca
      121 atgcgcggaa tttcaagcgc gcccggcacc cagtaaacgt ggatatcatt ttcatcaacg
      181 ccatgccgtt tcaaacagtc cagcgcaccg gcaagcaatt ctttagtaat catatcgtta
      241 aaccgactgg caacgatccc gactttcatt ttctcggcgg aaagcacgcc ttcgtgtagt
      301 ttaatcattt ggcacctttt tattatttaa gattaaatgt cccattttgt cccgtttagt
      361 tttgagatat ttttcatttt ggggatttgg tggaatctcg atcggaatgc gctccactac
      421 ttcgagatta aatccctgca agccaacaat tttctttggg ttattggtca gcagtcgcat
      481 cttatgtact cccagatcag ccagaatttg ggcacccatg ccgtaatcac gcaaatccgg
      541 cagaaaaccc aatttgatat tagcctcgac tgtatccatg ccttcatcct gcagtttata
      601 agccgaaatt ttatgacgta aaccgatccc gcgtccctcc tgccgtaaat aaagcaagac
      661 accgcgtttg ttctgcgcaa ttatctccag cgctttgtgc aactgatcac cgcaatcaca
      721 ccgctctgag ctaaaaacat cgccagtcag acattcagaa tgcacccgaa caatcaccgg
      781 ttcatccgat tttatatctc ccataaccaa agccacatgt tctttatcgg taagccggtc
      841 aacataaata atgattcgaa aaacaccaaa tttagtcggc aaatcggctt cactcacccg
      901 ctcgataaag cgttccgttc ttcgtcggta ttcaatcaaa ctctgaacgg taataatttt
      961 aacgttatga gtctgggcga ttttctccaa tgcttcacca cgcgccattt cgccattttc
     1021 gtccataatt tcgcacatca cactgacggg agtcaatcca gccaatcgca tcaaatccac
     1081 tgaggcttct gtatgaccag cacgtcgcag gactccaccc gggcgggcaa taatcgggaa
     1141 aatatgtccc gggcgcccta aatcttccgg tttggtattc ggattagcga gggcattaat
     1201 cgtcaaagcg cgatcgttag ccgaaattcc ggtcgtcgtg ccttccttta aatccacact
     1261 aaccgtaaag cgagtattat gcagagcggt attatcctgg accatcaatt ctaaattaag
     1321 ttctttcgct cgctcctccg tcaaacctac acacagcaga ccgcgcgcat attttgccat
     1381 aaaattgacc gattctggcg ttactttttc agccgccatg ataaaatcgc cttcgttttc
     1441 gcgattttca tcatcaacga cgatgacaaa gcgccccttt tttagatctt cgacggcggc
     1501 atctattgag tcaaatttca tcaaatcctc ttaatatctt aaaattgcta ttgattaaat
     1561 ccccattgtt taattttttc aaaagttagc gtttctttct caaagtgctc aagaattttt
     1621 tcaatatact tagctaatat atcaacctca aggttaactt cgtcgccaat ttttttctcg
     1681 ccgagattag tttcggtgag cgttactgga atcaaggcta tttcaattac ctcattctca
     1741 attccagcca ccgtcaaact aacaccatta accgccaaag agcccttctc gacgatataa
     1801 tgccgaatcg aatccggtac ggcgattcgc atagtacccg cattgcctat acgttggaaa
     1861 gcgacaatcg gcgcaacgct atctatgtgc ccctgcacaa aatgtcctcc aaaacgtcca
     1921 cttgccgcca tagcgcgttc cagattaact tttgcaccac ggggcacctt agccaacgtc
     1981 gtccgcgcaa gtgtctcagt gaccgcctgg acttcaatat aatctttacc aactttttca
     2041 actgtcaaac aaacaccatc aacggcgatg ctgtcaccga tattaatgcc gtctaaaact
     2101 cgatttgctg caatctggaa acggcgtcct gcgacattct cggagacggc tcgaatttca
     2161 cccatttctt cgataattcc agtaaacatt agactaattt cccttgtaga agcacatccg
     2221 ctccaagacg ctcgactttt tcccattcaa ccatcaaagc ctgatccagt gattgcataa
     2281 atccggaaaa cgcccgcaaa ccttcaccaa gaattttcgg tgcaataaat acttgtaatt
     2341 catccactag gcgttgattc aacagcgatc ccgccacctg actaccgcct tcgcaaaaaa
     2401 tggaagtaac accaaactca gccagtttag caagagtttt cggcaaatcc aaccagccaa
     2461 attcatcttt cgaactttga ataattttaa taccggatga ttcaaactgg cgaattttct
     2521 ccgctttttc ttgacaagca gtaacgatta tcgttcgccg ttgaaaacca tcggtcacta
     2581 atttcatccg cggtggcaaa ttcaaagctt cgtccagcac aactcgataa ggaatatatc
     2641 cggaacgatt ctcgggaagt aactccgggt cgtctttgta aaccgtgccc attccaacta
     2701 ccaccgcatc atattgttgt ctttgcttat gaacaaactc ccgcgcttca acaccggtaa
     2761 tccagcggct tttaccggta gcatcggcta taaaaccatc caaagtcaga gccattttca
     2821 gcgtaatcca aggacgtttt ttttgaataa aggtaaaaaa gccgcgattg gcagagcgag
     2881 cttccctttc caaaataccg acttgaactt ttagaccggc ttgttcgaga ctcttaactc
     2941 cagcgccgtt aacattggga ttgggatcga ttgtaccgat ataaacttca ctaattcctg
     3001 atttaataat agcctcggta cagggcgggg tctttccata gaaacaacac ggttcgagat
     3061 taacgtataa tatcgcacca cgcgcagatt caccggcttg ctctaatgct ttgatttcgg
     3121 cgtgaggagc accgaactta ctgtggaaac cttcaccgac aactttttcg ccggagacaa
     3181 tcacggctcc tacgaccgga ttaggactgg taaacccgcg tcctttttcc gccagttcca
     3241 gagctcgttt tagccatttc tcattttgat tcataaaaaa acccgattgc gctcgggatt
     3301 tttcatcttc tctcatccgg acttttaccg tcggtgtctg agtttcacag actctgtccc
     3361 gcctcaacgg aactcgcgga ctttcaccgc cggtcaggat ttacaccctg ccccgaagac
     3421 gcccacaaat ttataataca atcaacgaag ttccaatact gtaaaaatgt tttcgagttc
     3481 aaaccagcaa atctaccgcc agcagacagg tcatcaaggc aaaatctact agctgtttaa
     3541 tttccacata ttcattggca acgtgaaacg cattatcgtc acccggtccc cagccaaccg
     3601 ccgcaatacc gttcaaacaa agcgttttgg cataggtacc gccgccctga ccaatggctt
     3661 gcggttcgat attgagatat tctctagcgt tcgcctgaat tttgcgcact aattcgacat
     3721 ccgggctaat ggcgtgaggt tgattagtag aaataatttc aactttaaaa ttaccgttag
     3781 gaactttggc tatgcactcc tgaatctgct ctaataaaat ctcctccgtc tgacccggca
     3841 cagaacgtat atcgaggtaa atatagcaag aaccgggcac aatattggaa gccactccgc
     3901 cgtgaatttc acctaaattg agagttggat taccaagcac cggatgaact tcatatttga
     3961 acttcaaacg ttcaagttca tcaactaatt tcgccatcat aaaaatagca ttgatgccct
     4021 tttccggcgt agagccgtgc gcctgcttgc cgatggcggt aatcttaatg accgtacgac
     4081 ctttttcggc gacatcaatt tccagcatat tgccgccaat atccggcaca atcgccatcg
     4141 tcggattaat caaattctct tccacaagat agccgatacc ataattgatc ccgtctgggt
     4201 cttctgcttc ttcatcagaa agtgccgcaa ccattaattc gccagttaat tgctgatcaa
     4261 ttccgagttt tttcaaaatt tgagccgcta tcaggattga tgctagcggg cctttattgt
     4321 caagagtccc gcggccatag agtttgcctt ctttctcaac aacttcaaat gggtcggtta
     4381 gccaaccttc ccccgctgga acgacgtcca tgtgagccgg catcaggagg cgctgtccac
     4441 ttttattctg acctaaacgc gatataacat tcggacggcc ttccgctcga gcaaaaattt
     4501 cgtagggaat cccagcttta tccaattccc gttgaacgat ttcagctatt cggtactctt
     4561 caccgcgcat ttttaggaat gggaattcag gcaatttttc ggttacaaca ttaatcgttt
     4621 tttgtcgtac catttccgtg accagagcga tgatttggga tcgaacgtcc gcttgattgg
     4681 atttgaaata ttcgactaat tgcgctttga gcttcataaa gcctcctgat aatttaaatg
     4741 cgacattctt gtgagaaatt agttgatttg caaatactta tcaacaggta aaaatttaaa
     4801 aatcccgaca tattcttaaa acgaccataa attcactcaa tgaagtagag ggctacccca
     4861 ccgacggcga taatcgctcc tgccacttct ctaaatgtta ttttttcttt ctttattaaa
     4921 acttctaaag gaattatcaa aaccggcaca atagccataa tcgtggaagc gattccggtt
     4981 atagtacgtt gaacggcata gagcgagagt gagaccccca gaaatggtcc gataaacgct
     5041 ccgatagttg tgtacttcat agcgtctcga ttatgaacta ctttggccaa aagcggaaag
     5101 cgcttcaaga ttaaaaaaat tacagcaaaa ccgatcatac cagtaatgac ccgaatctga
     5161 gttgccccaa aagcgttgta atcactcata ccatatttgc taagaaccaa gccgacagcc
     5221 tgtccgattg cgcctcctaa agcgtataga attccggtcg ccggttgcga aaattttaat
     5281 ttgccgtttt cagtattccg cccgacaatt acaattgcca ccccgcaaat cgtcacgagc
     5341 attgccagta tttcccttag attcatcact tctccgagaa acaaccagcc gatcacagcc
     5401 gttatcagcg gtactaatga cattaccagc attgaaacgc gtgctccgac gattacgtaa
     5461 gcattgaata aaaaataatc gccaaatacg aagccaacca ggccggacaa agaaagccag
     5521 aaccaggcat gagcgctgaa cgatcgcggc caaaactgat gcctaaaaat accattgaac
     5581 agcgtcagga aaataaatgc cagtactaag cgtatgaaat taacctgtag ggaaccgatc
     5641 cgcttaccag ccgcctcgaa tgaaagtgaa gaaaaagacc agcatatagc aaccagcaga
     5701 gcggctaatt ctcccaaata ggcattactc atcgcgttcg gcatcaaatt taaaaataaa
     5761 aaaatcggcg caatcttcgg agactacgcc gatttttaaa atgttcaatt atctgggacc
     5821 aattcccaac caggttccga taaccggtcc gaacttgact gctaatccga cagttacgat
     5881 cgaaaccgtt gacatcaatt taatcacgat attgagacat ggaccggcag tatccttgca
     5941 aggatcgccg accgtatcgc ccactactgc ggctttatgt gcatccgaac ctttgccgcc
     6001 taaattacct tcctcgatat atttcttggc gttgtcccag gcaccgcccg aattgttcag
     6061 cataaccgcc aaaacaaaac cagtcgtcaa accaccggct aatacgccca atacaccggc
     6121 aactcccaaa attaggccgg tcaaaaccgg tacagaaatc accaacagtg atggtaaaat
     6181 catttcgcgc tgagcgcctt tggtgctgat tgctacgcaa ctggcgtaat cgggttcgtc
     6241 cttgccttcc ataattccag gcttttcacg gaattgacgg cgcacttcct cgaccatatt
     6301 accagcggca cgaccaactg ctttcatcgc taaagccgca aaaacgaaag tcaccattga
     6361 gccgagaaac attcctacca gaacgcgcgg attcattaaa gtaacgttat aatactcaat
     6421 ccattgcggt aggtgcagat tgttcgccac caactcctgc aaggtcttca gtgaagcacc
     6481 ggggtcaaac tgaatattta cttcactcag caagttctgt aaaatcgccg gattattgat
     6541 aatgtgggaa aattgagctt taatctcttc aatataagcg gcaatcaaag ccatagccgt
     6601 caaagcggct gagccaatgg caaagccctt gccggtcgcg gcagtagtat tacccagcga
     6661 atccagagcg tccgtacgct ggcgcacttc tttcggcaat ccgctcattt cggcatttcc
     6721 accggcatta tcggcaattg gtccgtaagc gtcggtcgcc aaggtaattc ccaatgtaga
     6781 gagcatccca actgccgcaa agctgattcc ataaagccca agatttatgt gattcggatc
     6841 agcaccaaat ccacccgcaa aagcataact caagataatt ccaataccgg cggtcagaac
     6901 cggtaatccc gtcgaaagca taccagtcga aagcccggca ataatgactg tcgccggacc
     6961 ggttttggac tgttcagcga tccagcgcgt cggtttataa ctttgtgagg tgtagtactc
     7021 agttattttt ccaattgtcc agccgaccaa taatcccgtt ataacggaag ccacgatgcc
     7081 catcggcaat ttcagaaagt acatcaaaat tgcagtaaat atcgcaatta aaacgacact
     7141 tagattgact ccgcgggaaa gcgctttcaa taatgttcgt tgatcggctt tttcttcggt
     7201 gcggacggca aaagcgccga tcacagaaaa aatattacca actccagcga cgatcattgg
     7261 ggctaaaagt gccgccaaaa ttttttgatg cggctgtccc tgaaaagcgg ctactccaag
     7321 cgccgctgtg gctaaaatcg aaccgcaata agattcataa agatcggcgc ccattccggc
     7381 gacatcaccg acgttgtcgc cgacgttgtc ggcaatcgtg gctggattgc gggggtcatc
     7441 ttctggaatt ccggcttcta ctttgccaac caaatctgct ccgacgtcag cggctttagt
     7501 aaaaatacca ccgcctactc gagcaaacaa cgcctgtaaa gaagcgccca tcccaaatgt
     7561 caacatcgta gtcgtaatcg ccgccagact catctggtcg aatcccagac ttggtaccaa
     7621 ccagtataga atcgcaaacc acatcgaaat atccaacaat cccaaaccca caactactaa
     7681 gcccattacg gcaccggaac gaaaggcaat ttgaagtccg gcgttcagtg atttacgagc
     7741 accttgggca gtgcgattgg atgccagtgt tgccgtactc attccgaaat aaccggctaa
     7801 agccgaaaag aaaccacctg tcaggaatga aaatggcacc catttattct gaacattaag
     7861 tccgaatgac ataatcagaa ataacacaaa aataatgaga aagaataatg ccaccacttt
     7921 atattgctgt ttaaggtagg caaaagcgcc ctcgcgaact gctaaggcaa ttttctgcat
     7981 ttggtcatta cccggatcat ttttcaacat cagtttataa aaaatccagg cggtaaccag
     8041 ggcaatgatc gaaccaattg gtgcaattaa aaataaatag tttagattca ttcttaacaa
     8101 acctccttta agttgtgact aaaatcgtgc ggacccaatc cgaccccaaa ttacttttac
     8161 tgaaaattat catcattaaa ataactcgga aatatataac tatttcagct aattctcaaa
     8221 aaattatctg aaaaataaac cataaaacca actctcattg gaatatattt ccaaaataat
     8281 cagaatctaa ctcatttttg atttaaatct ttttgtttac tagaattttc gctattttat
     8341 atcatattaa ggagtttaat atgaaacgat ttggtctttc ttacaagtta atcttagcct
     8401 cgatagttgt gctctttatg ataactctta cttgggcgga aaagccaact ttagttatta
     8461 tggatttcga ggcaaatgga attccacccg aagaggcaaa ggtttttacc gaacacctaa
     8521 aagtcgaatt atttaacagc gaaaactatt ctatcattga aaagtccggg attaaccaga
     8581 agcttcagga agaaggattc tccggcgaca aatgtgctac agttgattgc ctggttcagg
     8641 ctggtaaatt ctgcggagca gaacaggcag ttggtggctc aattacaaaa atcggcgata
     8701 cttattcggt tatggttcgt ttagttgacg ttaaccatag tcaactgcta aaaacatttg
     8761 cttatgatca taaaggcgat attatcagcc tcctctccga tggtacatca aaagttgcgg
     8821 cgaaaattag tgaaccgaag aagttaaacg ctgaaccggt ggttgctgaa aaagtcggca
     8881 cggtagtcga ggatacggtt attattgctc ccgcacgcgg caaaggtgga attgttccgg
     8941 cgctggcaag ttgtatgatt ggaccgcggg ttggattaga gatgaacgaa ggcaatgata
     9001 caatttatca aagtgaatgg atcgcattag gtggttccgt tgtcggcagt gcgttgactg
     9061 gttatttcgc accgttggga agtgcaattt cagttggaac acgagcctat atggcttatg
     9121 atatgggcgg taaaaacaac ggtctcgaag gtgccctcgc gagttattgc ctcgggcctc
     9181 gagtcggtaa tgaattacac tatcgtaaga ttcggacatt agagtggctt caactggttc
     9241 cctgtgtctg catttacccg atgattgccc ttccactgga agcctacaaa gg
//
LOCUS       DAOMJU010000085         9056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_8015_length_9056_cov_17.772581, whole genome shotgun sequence.
ACCESSION   DAOMJU010000085 DAOMJU010000000
VERSION     DAOMJU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8015_length_9056_cov_17.772581"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..2967)
                     /locus_tag="PLO58_07925"
     CDS             complement(265..2967)
                     /locus_tag="PLO58_07925"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HQO74755.1"
                     /translation="MEKWWQLNISQLCEKLSSDPEKGLSPEEASKRLTQYGPNQLREQ
                     KGRHPIFLFLDQFRDFIIWVLIGAAILSGFLGEWIDALAILAIVIFNAILGFVQEYRA
                     EKAIVALKKLSTPMAKVIRDGNQMTIPSNEVVPGDIIEIEAGDHIPADSRVLWHTSNF
                     RVEEASLTGESLPVQKNAESIEKPEIPLGERTNMVFAGTSVVSGKARAMVVSTGMQTE
                     LGKIADMILTIEHETTPLQKRLEAFGHWIVYLCFAIVAIVFVMELLRGGKLIDIFLTA
                     VSLAVAAIPEGLPAVVTIALALGVQRMVRRHVLIRKLPSVETLGSATVICSDKTGTLT
                     KNEMTVQSLYVGHRVYRVTGVGYNPQGEFFEDDKPLNREEIPLELEKALRCAVLCNGA
                     RLFKTEQGYGISGDPTEAALLVAGAKLGIWKEAEEKKYPFVDELPFDSNRKLMSMIRK
                     NGEQWVAFVKGAPDILLQRCDFIEINGSIRPLTEEDRIQIQEANQRFGNEALRVLGLA
                     YRTLDRIEKPFSTEQVENHLIFLGLIAMMDPPREEVKPAILACKTAGIQPVMITGDHK
                     TTAVAVAKALQIFSPETKALTGEELDQIDEATFQQEVKDISVYARVSPEHKLRIVRAW
                     KSRGDVVAMTGDGVNDAPAVKEADIGVAMGITGTDVTKEVSDMVVLDDNFASIVAAVE
                     EGRGIYDNIKKFIYYLLSCNAGEILMMFFSSLLGLPIPLFPIQILWVNLITDGFPALA
                     LGVEPLDPHVMERPPRPKDEGIVTKKTIFILLLQGFFIAFTSLLAFILVLKVENEGLA
                     RARTAAFIVLAVSQLFHSFNYRHFTESVFKVGFLGNKKLVLATGVSFLLQMAVVYIPF
                     LRTLFKTMPLLWWDWLLVIGLSSCTLWFMELVKWIWRKKGFSPF"
     assembly_gap    3460..3559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3560..>4692
                     /gene="gdhA"
                     /locus_tag="PLO58_07930"
     CDS             <3560..>4692
                     /gene="gdhA"
                     /locus_tag="PLO58_07930"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HQO74756.1"
                     /translation="ERVIIFRVPWVDDRGEVQVNVGYRVQFNSAIGPYKGGLRFHPAV
                     TLSMMKFLAFEQTFKNALTTLPMGGGKGGSDFNPTGKSENEIMRFCQSFMTELHRHIG
                     PYLDVPAGDVGVGGREIGYLFGQYKRIVNRFEAATLTGKGLAFGGSMLRPEATGYGAV
                     YFVVEMLKTKGETIKGKIVGVSGFGNVAWGVAKKATELGAKVISLSGPDGCIIDPDGV
                     GTEEKWNYMLEMRASGRDVVKDYADKFGVKFYPKARPWDIVPMDIAMPCAFQNEVSLE
                     NVQNLIKNGCQILAEVSNMGCTPEAFKLANAKMMYAPGKAVNAGGVAVSGLEMSQNSE
                     RLSWSAEEVDEKLKWIMTNIHSQCLAAAAEVGAPGNYAKGANIA"
     assembly_gap    4693..4792
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4793..5522
                     /locus_tag="PLO58_07935"
     CDS             <4793..5522
                     /locus_tag="PLO58_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HQO74757.1"
                     /translation="IDQGKRQDFMCGRKTLTKGKMEIIAELSIKEWDNSLDIQPNYNI
                     APTSIMPILVYENGRIVRAMRWGLIPEWAKDATSVPIMINARCESLTVKPSFRGLLDS
                     QRCIVITDGYYEWQKTESRKQPFYIHHPEKRLLTMAGLYSRWVASDSKLYLTYTVITT
                     PANAQLQFIHQRMPAILTPEEIDTWINCRQYDRQKALTLLQPSRMPLIAYPVATFVNS
                     VKNNSPQCIQPINLTAEQTNLLND"
     gene            5515..7851
                     /gene="hypF"
                     /locus_tag="PLO58_07940"
     CDS             5515..7851
                     /gene="hypF"
                     /locus_tag="PLO58_07940"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HQO74758.1"
                     /translation="MISHKIIITGIVQGVGFRPFIYNLARQYQLTGWVRNNSHGVEIV
                     AQGEPIQLARFAEIIQNSPPPRCRIERFEVTQFITAEKFLEFRIVESQNEQMKSVQIS
                     PDLDVCPDCRREMFDSSNRRYLYPFINCTNCGPRFTIIQDVPYDRQLTTMHEFKMCPE
                     CQCEYDDPSNRRFHAQPNACPVCGPRLQLLDNASRELLTGGSADANRAVFEKVAELLR
                     DGRIIAVKGIGGFHLACDARNEAAVTSLRQRKFREDKPFAVMFPSLPDIAKYCKYSRE
                     EARLLNSVPHPIVLLQKLPGRDVASAVAPGNHYLGCFLPYSPLHYLLMYFFPYPLVLT
                     SGNVSDEPIAYRDDDALARLNRIADYFLIHNRKIHIRCDDSVYRIWNNKTYPLRRSRG
                     FAPSALPYKNGFVRPILACGPEQKNTFALAQSNHIYLSQHIGDLVNFEVLQSLKNGIS
                     HFKNIFAIEPEIVAYDLHPDYLSTKFALEYPDETPDGQKVIKIGVQHHHAHAVACLAE
                     NNGAEPAIGIILDGTGYGTDGIIWGGEILKVEMQRFERLGHLRPARLPGGSAAIKYPW
                     QMAVSYLYETLGSEITIPKFPFLENVPPQQIGIVNDMLKSSFNSPLTTSCGRLFDGVA
                     ALAGLRNRVNYEGQAAVEFEQCIASENYRSAYDFEIETGTDSFVIDWRVMIEQVIEDV
                     KLQTGIELIAVKFHNGLANILLQAVLKSQEMTGLNTVALSGGVFMNIYLLTRLSQLLN
                     EHNFKVCTHHLVPCNDGGIALGQAVIANAIIQNQLEKS"
     gene            7851..8087
                     /locus_tag="PLO58_07945"
     CDS             7851..8087
                     /locus_tag="PLO58_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HQO74759.1"
                     /translation="MCLAVPMRIIEIDGSRAKAEVGGVLYQANLDFLSDVKVGDYIIV
                     HAGFAIEKLDEIVALDNLAAWQEVATDNKTKYRK"
     gene            8084..>9056
                     /gene="hypD"
                     /locus_tag="PLO58_07950"
     CDS             8084..>9056
                     /gene="hypD"
                     /locus_tag="PLO58_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HQO74760.1"
                     /translation="MIKYIDEYRNPELITKAVERIRAITPNKIVNLMEVCGGHTITIF
                     KFGLKKLLPENIHLISGPGCPVCVTDSEFIDRAIEIAKFPDVIITTFGDMVRVPGSYG
                     SLQSARAGGADVRTCYSPLEAVEIAERNPAKEVVFLGIGFETTAPNIASAILNANSRH
                     ISNFSVMSGHKTMPKAMQSLLDSGEIRLDGFICPGHVTAITGPEIYQFIADEYKIPCV
                     VAGFEPLDMLEAIFRLIKQIVERRAEVENQYRRAVKPGGNRKAQRIMAEVFEPCDTIW
                     RGMGNIPRSGLQIRSKYRQFDADYKFQVKLPPTKSVSGCICGDIMRGV"
BASE COUNT         2478 a         1898 c         1986 g         2494 t
ORIGIN      
        1 attgtgttag gtattttcat cattttgcag agaaaagaaa caaaaaagta actaattgaa
       61 aagcataaaa attcattagc ttttgtctac ttcaatttgg ggcttattct ttgtatactg
      121 gaagaattgc ccttgtctta atgtcgggga ttctctcatc tgaataaaag accatcaaga
      181 aacatttaat aaggacaatc cagccgaaaa tgacacggtt aagcatcgca ttgccaaaat
      241 ttaatatcta aaatccgtgt atttctaaaa aggtgagaat cccttttttc tccaaatcca
      301 tttaacaagt tccataaacc agagggtaca agaagaaagt cctatcacaa gaagccaatc
      361 ccaccacagt aagggcatag ttttgaacag agttcttaga aaaggaatat aaactactgc
      421 catctgaagg agaaaagaaa ctccggtcgc cagaacgagt tttttattcc ccaaaaatcc
      481 gactttaaac accgattcag taaaatggcg ataattaaac gaatggaaaa gttggctcac
      541 ggcaaggaca ataaaagccg cggttcgagc ccgagccaga ccttcattct ctacttttaa
      601 cacaaggata aaggcgagta aactagtgaa tgcgataaaa aatccttgga gcaggagaat
      661 aaaaatggtt ttcttagtaa caatgccctc atctttggga cgcggtggtc tttccattac
      721 atggggatcc agcggctcga ccccgagagc cagggcggga aatccatctg tgatcaaatt
      781 cacccacaaa atttgaatcg ggaatagagg aataggcaag cccaagagag aagaaaaaaa
      841 catcatcaga atttctccgg cattacagga aagcaggtaa taaatgaatt ttttaatgtt
      901 atcatatatt cctcggcctt cttctacggc ggcaacgata gaagcaaaat tatcatccag
      961 tacgaccatg tccgacactt ctttggttac atctgtgcct gtgattccca tagccactcc
     1021 aatatcggct tctttcactg ccggagcatc atttacgcca tctccagtca tagcgactac
     1081 atcaccacgg gatttccaag ctcgaacaat gcggagtttg tgttcgggag aaacgcgggc
     1141 ataaaccgaa atgtccttaa cttcttgctg gaaagtggct tcatctattt gatccaattc
     1201 ttcgccagtc aaggctttgg tttcggggct aaaaatttga agggctttag ctactgccac
     1261 ggccgtggtt ttgtgatcgc cagtgatcat taccggttga atgcctgcgg tcttacatgc
     1321 caaaatggct ggctttactt cctctcgggg aggatccatc atagcaatta accctagaaa
     1381 aatcaagtga ttttctactt gttctgtgga aaaaggcttc tcgatgcgat ccagcgttcg
     1441 atacgccaaa cccaatactc tcaacgcttc gttaccaaat cgctgatttg cttcttgaat
     1501 ttggattcga tcttcttctg tcaaagggcg gatagatccg ttgatttcaa taaaatcaca
     1561 tcgttggagc aatatatcgg gagcgccttt cacgaaagca acccattgtt caccattttt
     1621 ccgaatcata ctcataagct tccgattcga atcaaaaggc agttcatcta caaatggata
     1681 ttttttttcc tctgcttcct tccaaattcc taatttggct ccggcaacta acaacgcggc
     1741 ttccgtgggg tcacctgaaa ttccatagcc ctgttccgtt ttaaaaagac gggctccatt
     1801 gcataatacg gcacaccgaa gggctttttc taattctaat ggaatctctt ctctatttag
     1861 gggtttatca tcctcaaaaa attctccttg agggttatat cctactccag ttacccgata
     1921 tactcgatgt cccacataca gggactgaac ggtcatttca tttttggtca aagtaccggt
     1981 tttatctgaa caaattacgg tggcagagcc caaagtttcc acagaaggga gcttccgaat
     2041 gagaacatgg cgtcgcacca tacgctgaac ccctaaagcc aatgcgatag ttaccacggc
     2101 tggtaatcct tctggaattg ccgccaccgc aagactcacg gctgtaagga atatatcaat
     2161 aagtttgccc ccgcggagca attccataac gaatactatc gctacaatgg caaagcacaa
     2221 ataaacaatc caatgaccaa atgcttcgag tcgtttttgc agaggcgtgg tttcgtgctc
     2281 aatggtgaga atcatatctg cgattttgcc caactctgtc tgcatcccgg tactaactac
     2341 catagctctg gcttttccac tgacgactga agtccccgca aaaaccatat tcgtacgttc
     2401 tcccaatgga atttcaggtt tttcaatgga ttcagcattt ttttgaacgg ggagcgattc
     2461 cccggtcaaa ctagcttctt cgacacggaa attcgaagta tgccagagca ctcgggaatc
     2521 agccggaatg tgatctcctg cttcgatttc gattatatcg ccgggaacta cttcgtttga
     2581 aggaatagtc atttgatttc catctcgaat caccttagcc ataggagtgg acaatttctt
     2641 taatgccaca attgcttttt ccgcccggta ttcctggaca aaccccaaaa tggcattgaa
     2701 aatgacgatg gcgagaatgg ctagggcatc aatccattcc ccgagaaaac ccgataaaat
     2761 agccgcccca atcaataccc agatgataaa atctcgaaat tgatccaaaa atagaaaaat
     2821 cgggtgtctg cccttctgtt ctcggagttg attaggacca tattgagtaa gacgttttga
     2881 ggcttcttct ggcgaaagcc ccttttcagg atccgaagaa agtttttcac acaattgaga
     2941 aatgttcaat tgccaccatt tttccataat gctattccct tgttagaatt ttattcataa
     3001 gcgattgcgt gttgtttaaa agagatcggg ttttgtgata tataatgagg ttttatttcg
     3061 aaaaatgttg attttacaat ctactattaa gtccgaaagt tccctatttt cgcactcccc
     3121 aatccagggg taacaggttt ttaaaagata tgctcttagg gcagggcgct tttctaaata
     3181 aagataatgg actttgtttt gtataaaaca aaataagaga aaaattgtaa aaatagctcc
     3241 tatttcgctg tatatttttt ttatatcact atttctcata aaaaacaatt caatttaatg
     3301 acaggctacg gtgaaatcaa tccattttta tcaagactcc ctcaaaattt tagattgttt
     3361 aacctcaacg gaataaagat attttggagg agataatttc tgccttactc taaattcttc
     3421 ggtctatttt gatgccttat tggcaaacta tcatttatan nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnng aacgagtaat cattttccgg gttccctggg ttgatgaccg
     3601 tggtgaggtg caagtcaacg ttggttatcg agttcaattc aacagcgcga tcggtcccta
     3661 taaaggtggc ttacgttttc atccggcagt aacattaagt atgatgaaat ttttagcctt
     3721 tgagcaaact tttaaaaatg ctttaacgac tttgccaatg ggtggtggta agggtggctc
     3781 agatttcaat ccgaccggta aatccgagaa cgaaattatg aggttttgtc agagctttat
     3841 gacggaattg catcgtcata ttggtccgta ccttgatgtc cctgccggtg atgtaggagt
     3901 tggtggtcgc gaaattggtt atctttttgg acaatataag cggatagtga atcgctttga
     3961 ggcggctact ctgaccggca agggtttggc ttttggtggt agtatgctcc ggccggaagc
     4021 taccggctat ggtgccgtgt atttcgttgt cgaaatgctg aaaaccaaag gcgaaacaat
     4081 taagggaaaa atcgtgggtg tttcaggttt tgggaatgtg gcttggggag ttgctaaaaa
     4141 agctacggaa ttgggagcga aagtaatttc attatccggt ccggacggct gtatcattga
     4201 tcccgacgga gttggaactg aggaaaaatg gaattatatg cttgaaatgc gcgccagcgg
     4261 ccgagatgtt gtcaaagatt atgcggataa attcggcgtt aaattctatc cgaaagcccg
     4321 tccctgggac attgttccga tggacattgc aatgccttgt gcttttcaaa atgaagttag
     4381 ccttgaaaat gttcagaatt tgatcaagaa tggatgccag attctagctg aagtttcgaa
     4441 tatgggttgt actccggaag ccttcaaatt agcgaatgcg aaaatgatgt atgctcccgg
     4501 aaaagctgtc aatgccggtg gggtggcggt ttccggtctg gaaatgagcc agaattcgga
     4561 acgtttgtct tggagtgcgg aagaagttga cgagaagctg aaatggatta tgacaaatat
     4621 tcattcgcaa tgtcttgccg cagccgcgga agtcggagct cccggtaatt acgccaaagg
     4681 cgctaacatt gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaattgatc
     4801 aaggtaaaag gcaggatttt atgtgcggac gaaagacact gactaaaggc aagatggaaa
     4861 tcatcgcgga gctttccatc aaagagtggg ataattcact cgacattcag ccgaattata
     4921 acattgcgcc aaccagcatt atgccgattt tagtatatga gaatggacga atcgtgcggg
     4981 caatgcgctg gggtctaatt cccgaatggg caaaagatgc tacctcagtg ccaattatga
     5041 tcaatgcccg ctgtgaatcg ctaaccgtta agccttcttt taggggattg cttgattctc
     5101 agcgctgtat agtaattacc gacggctatt atgaatggca gaaaactgaa agtcgtaaac
     5161 aaccgttcta cattcatcat ccggaaaaga gactgctaac gatggctgga ctttatagtc
     5221 gttgggttgc gtcggatagc aagttatatc ttacttatac cgttatcacc actccggcta
     5281 acgcccaatt gcaatttatt caccaacgaa tgcctgcgat tctgacgccg gaagaaattg
     5341 atacctggat taattgtcgc caatatgatc ggcaaaaagc gctgacgctt ctccagccgt
     5401 cgcgaatgcc gctgatcgct tatccggttg cgactttcgt caattccgtg aaaaataatt
     5461 ctccccaatg tattcagccg attaatctaa ctgccgagca gaccaatctg ttaaatgatt
     5521 agtcataaaa taatcattac tggaattgta caaggagtcg gtttccgacc atttatttat
     5581 aatctcgcca ggcaatatca gcttaccggt tgggtgcgaa ataattcgca cggcgtggaa
     5641 attgttgctc agggcgaacc aattcaacta gctcgttttg cagagataat tcaaaattct
     5701 ccgccgccgc gctgtcgtat cgagcgcttc gaggtgacgc aattcataac ggctgagaaa
     5761 tttttagaat ttaggattgt tgaaagccaa aatgagcaaa tgaaaagtgt ccagatttct
     5821 cccgatctgg atgtttgtcc ggattgtcgc cgcgaaatgt tcgattcgtc caaccggcgt
     5881 tatctttatc cctttattaa ttgcacgaat tgtgggccgc gttttacgat tattcaggat
     5941 gtgccatacg atcgccaact gacgacgatg cacgaattca aaatgtgtcc ggaatgtcag
     6001 tgtgaatatg acgatccgtc caatcggcgt tttcatgctc agccaaatgc ctgtccagtc
     6061 tgcgggccgc gtctgcaatt gcttgataat gccagccgcg aattacttac cggcggcagt
     6121 gcagatgcta accgtgctgt ttttgaaaaa gttgctgaac tgttgcgcga tggtcggatt
     6181 atcgcggtca aaggtatcgg cggttttcac ttagcctgcg atgcccgcaa tgaagcggct
     6241 gtcacgtctc tacgtcaacg aaaattccgg gaggataaac cgtttgccgt tatgtttcca
     6301 agtcttccgg atattgctaa atattgtaaa tacagtaggg aggaagcgcg gctattgaat
     6361 agtgtgcctc acccgattgt tttactgcaa aaattgcctg gcagggatgt tgcttcggct
     6421 gtggcacctg gcaatcatta tctcggctgt tttctaccgt attcgccatt gcactattta
     6481 cttatgtatt ttttcccgta cccgttggtg cttaccagtg gcaatgtcag cgacgaacca
     6541 attgcttatc gagatgacga tgcacttgcc cgattgaacc ggattgccga ttatttttta
     6601 atacataatc gcaaaattca tatccgttgt gatgattcag tttatcgtat atggaataat
     6661 aaaacttatc ctctgcgacg ttcgcgtggt tttgcgccga gcgctctgcc ctataaaaat
     6721 ggattcgtcc gtccgatttt ggcttgtggt ccggaacaga agaacacttt tgcccttgcg
     6781 caaagcaatc atatatactt aagtcagcat atcggcgatt tagtgaattt tgaggtgtta
     6841 cagtcactta aaaatgggat tagccatttt aaaaatattt tcgctattga accggaaatc
     6901 gtagcctatg acttacatcc ggattacctc tcaactaaat tcgccctcga atatccagat
     6961 gaaactccgg atggccagaa ggtcattaaa attggtgtcc aacatcatca cgctcacgcc
     7021 gtcgcttgtc tggctgaaaa taatggcgct gaaccggcaa tcggcattat tctggacgga
     7081 accggctacg gcaccgacgg cataatctgg ggcggtgaaa tcctgaaagt ggaaatgcag
     7141 cgctttgaac gacttggaca cttgcgacca gctcgtctgc cgggtggttc agccgcaatt
     7201 aaatatccct ggcaaatggc agttagctat ctctacgaaa ctttgggttc ggaaattact
     7261 ataccaaaat ttccatttct ggaaaatgtt ccgccacaac aaatcgggat cgtgaatgac
     7321 atgctgaaat ctagtttcaa ctcgccacta acgacttcct gtggtcgcct tttcgacgga
     7381 gtagcggcgc tggcaggttt gcgtaatcgg gtaaattatg aaggccaagc ggcggtggaa
     7441 tttgaacaat gcatcgcatc tgaaaattat cgctcggctt atgactttga aatcgaaacc
     7501 ggaactgata gctttgtcat tgattggcgc gttatgattg aacaagtcat tgaggatgtc
     7561 aaacttcaaa ctggaatcga gctaatcgct gtcaaatttc ataacggatt ggcaaatatt
     7621 cttctgcaag ccgttttgaa atcccaagaa atgaccggcc ttaatacggt ggcgctaagc
     7681 ggaggcgttt ttatgaatat ttacttgctg acgcgtctat cgcaattgct taacgagcac
     7741 aatttcaaag tctgcactca tcatcttgta ccttgcaacg acggtggaat tgctctcggg
     7801 caagcggtta ttgccaacgc aattattcaa aaccaattgg agaagtcata atgtgtctgg
     7861 ctgtgccgat gagaattatt gaaattgatg gaagccgcgc caaagctgaa gtcggaggag
     7921 tcctatacca agccaacctc gactttttga gtgacgttaa agtcggtgat tatatcatcg
     7981 tacacgccgg ttttgccatt gagaaattgg atgaaatcgt ggcgctcgat aatctcgccg
     8041 cctggcagga agttgctacc gacaacaaga ccaaataccg aaaatgatta aatacattga
     8101 cgaataccgc aatccggaat taattaccaa agccgtcgag cgcattcgag caattacacc
     8161 gaataaaatt gttaatctaa tggaagtctg tggcggtcat acaattacca tttttaaatt
     8221 cggattgaaa aaattgctac cggagaatat ccatctgatt agcgggccag gctgtccagt
     8281 ctgtgtgacc gatagcgaat ttattgatcg ggcaatcgag attgctaaat tcccggacgt
     8341 cataattaca actttcggtg atatggtgcg agttcctgga agttacggta gtttgcagtc
     8401 agcccgagct gggggtgccg atgtgcgtac ctgctattcg ccgctcgagg cggttgaaat
     8461 cgctgagcgg aatccggcga aagaggtcgt tttccttgga atcggttttg agacgactgc
     8521 accgaatatt gcctcggcga ttttaaatgc taatagtcgg catatctcaa atttttcggt
     8581 gatgtcaggt cataaaacta tgccgaaagc aatgcaatct ctgctggatt ccggcgaaat
     8641 aaggttggat ggttttattt gcccgggaca tgttactgct ataactggcc ccgaaattta
     8701 ccaattcata gctgacgaat ataaaatccc atgtgttgta gctggatttg agccgctcga
     8761 tatgcttgag gcgattttta ggttaattaa acaaattgtc gaacggcggg cggaagtcga
     8821 aaatcagtat cgccgcgccg ttaaaccagg cggtaaccgc aaagcccaga gaataatggc
     8881 agaagttttt gaaccctgcg atacaatctg gcgcgggatg ggcaatattc ctaggagcgg
     8941 tcttcaaata cgatccaaat accgtcaatt tgatgccgat tataaatttc aggtaaaatt
     9001 gcctcccact aaaagtgttt caggttgtat ttgtggagat attatgcgtg gcgtca
//
LOCUS       DAOMJU010000086         8888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_8235_length_8888_cov_16.945092, whole genome shotgun sequence.
ACCESSION   DAOMJU010000086 DAOMJU010000000
VERSION     DAOMJU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8235_length_8888_cov_16.945092"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLO58_07955"
     CDS             <1..648
                     /locus_tag="PLO58_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQO74761.1"
                     /translation="TIRSMLAVIDEVYSDSLQPELKEMVERAVRRTDQVLMMVKEMIE
                     VTQYRYGLMEQKLDKVNFQEWIDKVVEQQQIYAANKKIALIVKKLPQSPAIYIDTVSM
                     EKVLSNLINNAIRYSPAGAEVTILPFVKQQCFGFCVRDTGIGIAPDELPKIFEEFYRS
                     ANAREMENLGTGLGLSLVKQIVEKYNGKIFVESKIGQGSTFTVEMPYLNLQINPK"
     gene            703..1125
                     /gene="nikR"
                     /locus_tag="PLO58_07960"
     CDS             703..1125
                     /gene="nikR"
                     /locus_tag="PLO58_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HQO74762.1"
                     /translation="MAGLIRFGVSLPEDLLTRYDNEIQRRGYSNRSEALRDLIREFLV
                     QKEVDSDAEVVGTLTLIYDHHVADLAAKLKSMQHESYQNIMANIHFHLDQHNCLEVIV
                     LRGRCSLIQNIAERLIGLRGVKHGKLTFTSTGKNLPGL"
     gene            1142..3007
                     /locus_tag="PLO58_07965"
     CDS             1142..3007
                     /locus_tag="PLO58_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO74763.1"
                     /translation="MAIKYALKYFLLITLMAVSLSSAAIYTDTLKVFPFDPVVVTGSR
                     TAINRHELPVAITVIPENVLSEQSGAPLLDLLSENVPGLFITRRTNLGYGLSSGAGGQ
                     ISMRGIGSFPTTQVLVLIDGRPDIMGLFGHPLGDVYFAENVKKIEVQRGPASLLYGSN
                     AMGGAINIITDHKSEPGYHLRMPIRFGSFNSHQLNLHQSFERKNWGVSGTVGAVSSDG
                     YRTDGRDDYSNQNGIAEAHWQIGNAWRADVNAYFSNMEIYDPGQTDNPFSNHRYDIKR
                     RGGDLTFNHSLAKLTSDLKLHYNYGHHDIDDPNKYISDDYTAGLILTETFYKSEKSRV
                     MVGIDLRQYGGKAWLDSTWKKHNVAEQSLITQFNRKLFSLVNLDGGLRYTHHSVAGAE
                     WIPALGMALHLPKGWYLKTQFARGYRNPTINELYLFMPSTTDLKPELSTNLEFIVEKE
                     IIGWLTTSLSLYHTKAENLIEKGWVSGMPIYQNRGEVVLKGIEWEGQLIITSNFSTSW
                     TMSVNTASQKVSGNPGQKCDLGLRWNPTEKLHLTLQSQWINNLYSLANPYSYGQPVYK
                     RLNGYWLLNWRGSYVINQFIDLSAEIENLTNVEYETMYHYPLPGRNFWIGITLKY"
     gene            3022..>3540
                     /locus_tag="PLO58_07970"
     CDS             3022..>3540
                     /locus_tag="PLO58_07970"
                     /inference="COORDINATES: protein motif:HMM:NF017001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQO74764.1"
                     /translation="MNMAIYKNIIKYFDAQATSWHLSGLDRIQAENILSSVQLPESGV
                     VLDAGCGAGNLFPILRKIVPLATIIACDISRKMLIESRQRFSNNVPLWVGYCEELPLK
                     DTSVDLILNYCIFPHIKNSHKALFEYRRVLKSGGHLLIVHPQGRQTINSKHRQIGFPI
                     SDDLLPPSAEIVE"
     assembly_gap    3541..3640
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3643..4362)
                     /locus_tag="PLO58_07975"
     CDS             complement(3643..4362)
                     /locus_tag="PLO58_07975"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQO74765.1"
                     /translation="MGSRIKTLLFLLSLLVILAMAKNPQYPDYYQLRQKMVNEQIIAR
                     GVRAESVIKAMQKVERHLFVPEQYRNFAYSDRPLPIGEGQTISQPYIVALMTELLDLK
                     KSDKVLEIGTGSGYQAAILAEICDSVYTIEIIPSLGKQAQALLRELGYHNIHCKIGDG
                     YLGWPEHAPYDGIIVTCAPSKIPQPLKEQLAEGGRMVIPVGATYTQELVLVTKTKGKL
                     IQKSVIPVRFVPMLRSPNGLD"
     gene            complement(4393..5088)
                     /locus_tag="PLO58_07980"
     CDS             complement(4393..5088)
                     /locus_tag="PLO58_07980"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HQO74766.1"
                     /translation="MSERKISGVKIAGLVLIILGVLLLLDNLNLIFFDLGDFLHNWWP
                     VILILIGLNELLKTNHSGGWFLIGLGIILTLKINDIIDGSLLIAMLLILIGIIIIIRP
                     RRFYPQKTADNSQPETNNSNNINLRAVFSESTENIISDNFSGGKIETIFGKMVVDLRP
                     AKMQPGRYHLNVETVFGQTILILPNEISAEVIGTPVFGRIDNQAKAIPRDESTAVLEI
                     RAEVVFGKIEIRQ"
     gene            5279..>6807
                     /locus_tag="PLO58_07985"
     CDS             5279..>6807
                     /locus_tag="PLO58_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HQO74767.1"
                     /translation="MKKFILMLFMVLMMMNCDSKIKQTEQMAQAFIDSLTNVIKPLNK
                     AANLAYWEATASGKDEYYERYAGASLELATVYANSADFEKLKTLKSSKIKDPLLARQI
                     EILYYRFLAKQIDTTLLKQITEMQTAVEARFNKFRGKIDGRAVTGNDIKEILVSSNDL
                     TLRKKAWEASKQVAPAVEKDMLALVHIRNEAARKLGFNNFQQMSLITDEQDPAEIERI
                     FQELDAATREPFRHDKAMMDSILSKRFNIKPEELRPWHYADPFFQDVPAVSAVNLDQY
                     YQNQNVVKIAESFYAGIGLDVSQILANSDLYERPGKYPHAYCTDIDREGDVRIMVNVR
                     NNEEWMATVLHELGHAVYSSNIDRQLPYFLREEAHLFTTEAIAMFFEKLSKNPTWMRA
                     MLNLDAEEEADIREATAQMLRLQKLIFARWSLVMYNFEKSLYENPDQDLNQLWWDLVE
                     QYQMIKRPENRNQPDWASKIHVCSYPVYYHNYQLGELLAAQLTEAIARYQGVNDGAKI
                     K"
     assembly_gap    6808..6907
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6908..8773)
                     /gene="pepF"
                     /locus_tag="PLO58_07990"
     CDS             complement(<6908..8773)
                     /gene="pepF"
                     /locus_tag="PLO58_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HQO74768.1"
                     /translation="MKQLFTFSVVIMLFLSNEETSVAQTRDRSQISEKHTWDLTPIYP
                     SDEAYLEAKDALVKKFDQILKYKGKLGQSADQLYACLNLNSEISKEMARLGSYISFKA
                     DLDTRDTKYLGMRQELSQLATEYYTKAAFIQPEILEIDPAKLKKFIASKKELKVFEFY
                     INDLIRVKKHLLSEKEEKIIAETGLFVEAPYTIYSLFSNAEFPFPEIVLSDGTKVKLD
                     LATFATLRALPVRADRELVFNSFFGKMYDFRRTFGATMDANVKQHLFIAHVRNYNSCL
                     ETALDANNIPTDVYTALIKNVNDNLPTFYRYLKIKQQLLGVDTLKYTDLYTPVVKDLD
                     IDYPIETAQQMILSALKPLGSDYLSVIERAFNERWIDIYPTPAKRSGAYSDGSIYDVH
                     PYVLLNYCNKYDDVATLAHELGHTMHSYFSNKTQPFPLSDYSIFVAEVASTFNEVLLF
                     HDNLNKVTDPDQKLTLLMNYLDGIKGTVFRQTQFAEFELRMHEMAERGEQLTGDSLTE
                     LYKQIVRMYYGHDKGICYVDDIINIEWAYIPHFYYNFYVYQYATSFTAATALAKKVLE
                     GDESARSNYLNFLSSGGSDYPINQLKAAGVDMTTAEPFNLTMQEMNWVMDEIEKVL"
BASE COUNT         2491 a         1732 c         1975 g         2490 t
ORIGIN      
        1 actattcgca gtatgctggc tgtaattgat gaggtttatt ctgacagtct ccaaccggaa
       61 ttaaaggaaa tggtcgagcg agctgtacga cgcactgatc aggttttaat gatggtcaaa
      121 gaaatgattg aggtaacgca gtaccgatat ggcttgatgg aacaaaagct ggataaggtg
      181 aattttcaag aatggattga caaagtcgtc gaacagcaac agatttatgc cgctaataaa
      241 aagattgcat tgatagtcaa gaaattaccc caatcgccag cgatttatat agacacggtc
      301 agtatggaga aggtgctttc taacttaatc aataatgcca ttcgctatag ccccgccgga
      361 gccgaagtaa cgatcctacc ctttgtcaaa cagcaatgtt tcggattttg tgtaagggat
      421 accgggatcg gtattgctcc ggatgaactg cctaaaattt ttgaggaatt ctatcgcagt
      481 gctaatgctc gtgaaatgga aaatctgggg accggtttag gattgagcct cgtcaaacaa
      541 atcgtcgaaa aatataacgg caaaatattt gtcgagagta aaatagggca agggagcacc
      601 tttaccgtcg aaatgcccta tttaaatttg caaattaatc caaaatgata tattttttga
      661 ctcgttgtgt tacaaatatg aaaatgttgc tacgaggtga atatggctgg cttaattaga
      721 ttcggagttt ctctaccgga agacctgcta acacgctacg acaatgaaat tcaacgcaga
      781 ggctattcta atcgttcgga agccttgcgt gacctaattc gggaattttt agtccagaag
      841 gaagttgatt ccgatgccga ggttgtcgga acattaacct tgatctatga tcatcacgtt
      901 gctgatttgg cggcaaaact gaagtctatg caacacgagt cataccagaa tattatggcg
      961 aatattcatt ttcatctcga tcaacataat tgtctcgagg taatcgtatt gcgcggtcgg
     1021 tgttcattaa tacagaatat agccgagcgg ctgatcggtt tgcgcggggt aaaacatggc
     1081 aaattgacct ttacatcaac aggaaaaaac ctaccgggtt tataacatca gggattaaaa
     1141 aatggcaatt aaatatgcgt taaaatattt cttattaatt accttaatgg cagtgagttt
     1201 aagctccgcg gcgatttata ccgatacgct gaaagttttt ccattcgatc cggtagtggt
     1261 aaccggatcg cgcacggcca tcaatcggca tgaactgccg gttgcaatca cggtcattcc
     1321 cgagaatgta ttgagcgaac aaagtggagc gccgcttctg gatttgttat ccgaaaatgt
     1381 gccgggattg tttatcacga ggcggacgaa tcttggatat ggattgagca gtggtgctgg
     1441 tggtcagatt tctatgagag gtatcggtag ttttccgact actcaggtgt tggttttaat
     1501 cgatggccgc ccggacatta tgggactttt cggacatccc ctgggcgatg tttattttgc
     1561 tgaaaatgtc aaaaagatcg aggttcaacg cggtccggct tcccttttat atggttcgaa
     1621 tgcgatggga ggagcgatta atatcatcac ggatcataag tccgagccgg gttaccattt
     1681 gcggatgcca atacgattcg gcagttttaa ttcacatcaa ttgaatctgc atcagtcgtt
     1741 cgagcgaaaa aactggggag tctcgggaac ggtcggagcg gttagcagtg acgggtatcg
     1801 aacagatggt cgagacgatt attccaatca aaacggcata gccgaagctc attggcaaat
     1861 tggcaatgcc tggcgggcag atgtcaatgc ctatttttcg aatatggaaa tttatgatcc
     1921 ggggcaaact gacaatcctt ttagcaatca tcgctatgat atcaaacggc gcggtggcga
     1981 tctgactttt aatcacagtc tggcgaagtt gaccagcgat ttaaaattgc attataatta
     2041 tggacatcat gatattgatg atccgaataa atacatatcg gatgattaca cagccggttt
     2101 gatattgacg gaaacatttt ataaatctga aaagtcacga gtaatggtcg ggatagattt
     2161 gcgtcagtac ggcggcaaag cctggttgga ttcgacctgg aaaaagcaca acgtcgccga
     2221 acaaagcctc attactcaat ttaatcgaaa gttattttca ttggttaatc ttgatggcgg
     2281 attgcgctat actcatcatt cggtcgctgg tgcagaatgg ataccagccc tcggtatggc
     2341 tctccattta ccaaaaggtt ggtatcttaa aactcaattc gccagaggct atcgcaatcc
     2401 gacgatcaat gaactttatt tatttatgcc gtcaaccacc gatttaaaac cagaactatc
     2461 gaccaacctg gagtttatcg tcgagaagga aatcattggt tggttaacga cgtctctatc
     2521 attatatcat acgaaagccg agaatttaat tgaaaaaggc tgggtctccg gtatgccgat
     2581 ttaccaaaat cggggtgaag tggttctaaa aggtatcgaa tgggaagggc aattaattat
     2641 cacctctaat ttttccactt cctggacgat gagtgtcaat accgcttccc agaaagttag
     2701 cggcaatccc ggtcaaaaat gcgatctcgg tttgcgctgg aatccgaccg aaaagctaca
     2761 tttgacttta cagagccaat ggattaacaa tctatatagc ctcgcaaatc cgtacagtta
     2821 tggtcagccg gtctataagc gcctaaacgg ttattggtta ttaaactggc gcggtagtta
     2881 cgtaatcaac caattcattg atctaagcgc cgaaattgag aacctgacca acgtggaata
     2941 tgaaactatg taccattatc ctttgccagg ccgcaacttt tggattggga taaccttgaa
     3001 atattgattt attttagaca gatgaatatg gcaatttata agaatataat taaatatttc
     3061 gatgcccagg cgactagctg gcatttatcc ggattggatc ggattcaagc cgagaatatt
     3121 ttgagttcag tccaattacc tgaatccgga gtggtgctgg atgccggttg cggagctgga
     3181 aatttatttc cgatattgag aaaaatagta ccgctggcta ctattattgc ctgcgatatt
     3241 tccagaaaaa tgctgatcga aagccgtcag cgtttctcga ataatgtacc actctgggtc
     3301 ggatattgcg aagagttgcc cttgaaagac accagtgttg atttgatcct gaattattgt
     3361 attttccccc atattaaaaa cagtcataaa gcgttattcg aatatcgccg cgttttaaaa
     3421 tccggcggac atctactgat agttcatcca cagggtcgcc agacgattaa ttccaagcat
     3481 cgtcaaatcg gatttccaat tagcgacgat ttattgccac cttcagccga aattgtggaa
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttttaatcta agccgtttgg
     3661 gcttctcagc atcggcacaa agcgcaccgg gatgactgat ttttggatta atttgccctt
     3721 agttttagtg accagaacta gttcctgtgt ataagttgca ccaaccggaa taaccattcg
     3781 accaccctcg gcgagttgtt ctttgagtgg ttggggaatt ttagatggcg cgcaggtgac
     3841 gattataccg tcgtacggcg catgttccgg ccagccgaga taaccatctc caattttgca
     3901 atggatgttg tgatagccca gctctctaag aagtgcctga gcttgctttc cgagtgaggg
     3961 gatgatttcg atcgtataaa cgctatcgca gatttcagcc agaattgccg cctgataacc
     4021 actgccggta ccgatttcga ggactttgtc gctctttttt aaatccagta gttcggtcat
     4081 cagagctaca atataaggtt gcgagatagt ttgcccttcg ccaatcggca aaggacgatc
     4141 ggagtaagca aaattccggt attgttcagg cacaaataga tggcgttcaa ccttttgcat
     4201 cgcttttatg acggattcag cccggacgcc acgcgcaata atttgctcat tcaccatttt
     4261 ctgccgtaat tggtaataat cgggatattg gggatttttt gccatagcca atattaccaa
     4321 aagacttaat aaaaatagta aggttttaat tctactaccc atttttctat caatgaatcc
     4381 tgactattca cattattggc gaatttctat cttgccgaaa actacttcag cccgaatttc
     4441 tagtaccgct gtgctttcgt ctctcggtat ggctttcgct tgattgtcta tccgtccgaa
     4501 aaccggcgtg ccgattactt cagctgaaat ttcattaggt aaaatcagga tcgtttgacc
     4561 gaacaccgtt tcgacgttga ggtgatagcg ccccggttgc attttagctg ggcgcaagtc
     4621 aactaccatt ttcccgaaaa tagtttcaat tttgccgcca ctgaagttgt ctgagataat
     4681 gttctcggtg gattcggaaa atacagctcg taagtttatg ttattgctat tattggtctc
     4741 gggttgagaa ttatcggcgg ttttctgagg ataaaatcgg cgaggacgaa ttattatgat
     4801 tattccgatt aaaatcaata gcattgcaat taatagagaa ccgtcaataa tatcattgat
     4861 tttcaatgtc agaattattc ccagtccaat taaaaaccaa ccgccggagt gattagtctt
     4921 gagtaattcg ttcaaaccga ttagaatcaa aattacaggc caccaattat gcaggaaatc
     4981 gcctaaatcg aaaaatatca ggtttagatt atccaaaagt aataaaactc ctaaaattat
     5041 aagaaccaat ccggctatct ttactcccga tattttgcgc tcactcataa taaacctttc
     5101 tctctttttt tcaattatac gaaatagttt aaactaaatc aatcggaaaa tcggttaatt
     5161 ttgattggtt aattcttgaa tcctcgtgac tttaatggta aattaaaaac cgaatatgca
     5221 cgatgaatat tataacaaga tgtatcagta gaaaatatct gcgaggagta tatccgatat
     5281 gaagaaattt atcttaatgt tatttatggt gttaatgatg atgaattgtg acagtaaaat
     5341 taaacagacc gaacagatgg cgcaggcttt tattgatagt ctgaccaatg ttattaagcc
     5401 gttgaataaa gcggctaatt tagcctactg ggaagccact gcttctggaa aagatgaata
     5461 ttatgaacgc tatgctggtg cttctttgga attggcgacg gtttatgcca attcagccga
     5521 tttcgaaaag ctaaaaacct taaaatccag caaaatcaaa gacccattat tagcgcggca
     5581 aatcgagatt ctttactatc gctttttagc caaacaaatt gatacaacct tgttaaagca
     5641 aatcaccgaa atgcaaacgg cggttgaagc tcgattcaat aaattccgtg gtaaaattga
     5701 cggtcgcgca gtcaccggca atgatatcaa ggaaattctg gtttcgtcaa acgaccttac
     5761 attacggaaa aaagcctggg aagcctccaa gcaggttgca ccggcagtcg aaaaagatat
     5821 gctggctctg gtgcatatcc ggaacgaagc cgctcgcaaa ctcggattca ataactttca
     5881 acagatgtcg ctgattaccg atgaacaaga tccagctgaa atcgagcgaa ttttccagga
     5941 actggatgcc gcaacgaggg aaccattccg tcatgataag gctatgatgg attcgattct
     6001 gtccaagcgt ttcaatatca aaccggaaga actacgtccc tggcattatg ccgatccctt
     6061 ttttcaggat gttccggctg ttagcgcggt taacctcgat caatattatc agaatcagaa
     6121 tgtggtaaaa atagccgaat cattttatgc cggaattggt ctggatgtat cgcaaattct
     6181 ggctaatagt gatttgtatg agcgtccggg caaatatccg catgcctatt gcactgatat
     6241 tgaccgtgaa ggcgatgtgc gcattatggt aaacgtgcgc aataatgaag aatggatggc
     6301 gactgttctg catgaactgg gacatgcggt ttattcgagc aatattgatc ggcaactgcc
     6361 ttacttttta cgtgaagaag ctcatctgtt cacgaccgaa gccattgcga tgtttttcga
     6421 gaaactatcg aagaatccga cctggatgcg ggcaatgctt aacctcgatg ccgaggagga
     6481 agccgatatt cgggaggcaa ccgcgcagat gttaagattg caaaaactga tttttgcccg
     6541 ctggtcgttg gtcatgtata attttgaaaa atctctgtat gagaatcccg accaggactt
     6601 gaatcaactc tggtgggacc tcgttgaaca atatcaaatg atcaagcggc cggaaaatcg
     6661 caaccagccg gattgggctt ccaagattca tgtttgttct tacccggttt attatcataa
     6721 ttaccagcta ggcgaacttt tagccgccca gttgaccgaa gcgatagcgc gctatcaggg
     6781 agttaacgat ggcgctaaaa ttaaatannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnngag gactttttca atttcatcca ttacccaatt catttcctgc attgttagat
     6961 tgaacggttc ggcagtagtc atatcaaccc cggcggcttt taactggttg atcgggtagt
     7021 cggaaccgcc ggaagagagg aaattcagat agttggaccg ggcggattcg tcaccttcta
     7081 atactttttt ggctaacgcg gtagcggctg tgaaggacgt ggcatattga taaacgtaaa
     7141 aattatagta gaagtgggga atataagccc attcgatgtt gatgatgtca tccacgtagc
     7201 aaataccttt gtcgtggcca taatacatcc gcacgatttg tttataaagt tcagttagtg
     7261 aatcaccggt caactgctca ccgcgttccg ccatttcatg catacgcaat tcaaactcgg
     7321 cgaactgcgt ttggcgaaaa accgtgcctt tgatgccgtc taaataattc atcaacagag
     7381 tgagtttctg atcgggatca gtgactttat tgagattatc atgaaaaagc agaacctcgt
     7441 tgaaggtcga agcaacttca gcgacgaaaa tcgagtagtc ggaaagcgga aaaggttggg
     7501 ttttattgga aaagtagcta tgcatagtgt gaccgagttc gtgcgccagg gtcgcgacgt
     7561 catcgtattt attacagtaa ttgagcaaaa cgtagggatg cacgtcgtaa attgagccgt
     7621 cggaataggc gccactccgt ttagcaggag ttgggtaaat atcaatccaa cgctcgttaa
     7681 aagcccgctc aataacgctg agatagtcac ttcctagcgg cttcagagcg cttaaaatca
     7741 tttgctgagc agtttcaatg ggataatcaa tatccaaatc cttgacaact ggtgtgtaga
     7801 ggtcggtata tttcaatgta tctacaccga gcaactgctg tttaatttta aggtaacgat
     7861 aaaaagtcgg tagattgtca ttaacgtttt ttatcaaagc cgtatagaca tcggtaggaa
     7921 tgttatttgc atcgagggcg gtttccaaac aactgttgta attacgaacg tgagcgataa
     7981 aaaggtgctg tttgacgttg gcgtccatag ttgccccgaa ggtgcgacgg aaatcataca
     8041 ttttgccaaa gaaagagttg aaaactaatt cgcgatcggc tcgcactggc agagcgcgca
     8101 aggtagcaaa ggtagccaga tcgagtttga cttttgtgcc gtcgcttaaa acgatttccg
     8161 ggaaagggaa ttcagcattg ctaaaaagac tatagatcgt atagggcgcc tcaacgaata
     8221 agccggtttc ggcgataatt ttttcttctt tttcggaaag caggtgcttt ttaactcgga
     8281 tcaggtcatt gatataaaac tcgaagactt tcaattcttt tttggaggcg atgaattttt
     8341 tcaacttagc cgggtcaatt tcaagaattt caggttgaat aaaagccgcc ttcgtataat
     8401 attcggtcgc aagttgggag agttcctggc gcatgcccag gtatttcgtg tcgcgggtat
     8461 cgaggtcggc tttgaaactg atatatgagc ccagtcgcgc catttccttg ctaatttcgc
     8521 tgttgaggtt aaggcaggca tagagctggt cagccgactg cccgagtttg cctttatact
     8581 tcagaatctg gtcaaatttt ttaacgagag cgtctttggc ttctaaatag gcttcgtctg
     8641 atgggtaaat cggtgtcaag tcccaggtat gtttttccga aatctgggag cggtcgcgcg
     8701 tctgcgcaac ggaagtttct tcattagata agaatagcat aatcaccacg ctgaaagtaa
     8761 atagttgttt catttttatc ctctttgttt taatagttac ctattatatt tgaagcggaa
     8821 atgctgattt ggtttctgaa aatatttgat ttagatggag aaaccttact ccatttaaat
     8881 tggtttta
//
LOCUS       DAOMJU010000087         8829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_8302_length_8829_cov_26.566674, whole genome shotgun sequence.
ACCESSION   DAOMJU010000087 DAOMJU010000000
VERSION     DAOMJU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8302_length_8829_cov_26.566674"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="PLO58_07995"
     CDS             <1..1280
                     /locus_tag="PLO58_07995"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HQO74769.1"
                     /translation="LACQCQVTEPLVIELAQLEIPILSDVTRIKLVGESGLAVVVDLG
                     TTTLVAQLLDLSSGIVLGVETALNPQARHGADIMSRIQFGLTTAGRELLQNLIRHQIG
                     QMITRLVEKCRCPERRLKQINIVGNSVMHHLFGGLSVEPLALLPFEIEQPGELEWSAE
                     EIGWQLPGNPKVKFLPCLGGFVGSDILAGILAVGMHRSPETVALIDLGTNGEIVIGNR
                     DRILCASTAAGPAFEGAQITMGMRATTGAIWKVWLTNGQLQVEVIGNVPARGICGSGL
                     VDAVAAALDLGWIDPTGRITLPKREIPLQKPVVITQSDIRELQLAKGAIAAGIQILRK
                     QMQLKPADIKQVFLAGAFGNYINVVSARRIGLFDFPTELIEQAGNTALHGAKLSLLNE
                     QEYHSIPLITKHFPLATDAQFMEIFAENMNFPG"
     gene            1665..3011
                     /locus_tag="PLO58_08000"
     CDS             1665..3011
                     /locus_tag="PLO58_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HQO74770.1"
                     /translation="MLIKIKKGLNLPIAGAPEQVIYPAQSVSQVALLGLDYVGLKPQF
                     AVTIGDQVKLGQPLFSDKTYPQIRFTAPGAGQVIAINRGTKRAFLSLVIKLDGDDAVE
                     FKSFSEQQIGSLTQEEIINQLLESGLWPSFKARPFGKLANPESQPHSIFVTAMDTNPL
                     APDVAVILREQSNDFQRGLALIAKLTAGKVFVCQAPDTAIPVPEIENIEIVEFAGPHP
                     AGLAGTHIHLLDPVSLHKTVWSIGAQDVAAIGKLFTTGRLPTARIVALAGSAVKKPRL
                     ITTRVGANLSELTDGELAEGHNRIISGSVLAGHTAAGELNFLGRFHQIVSVIPEGGRR
                     KLFGWLSPGWRLFSVKNIALAAFLPNRKLVFDTDTHGSERAIVPIGSYEKVMPLDILP
                     TYLLRALAVDDLEEAEKLGCLELVEEDLALCTFVCPSKIDHGQNLRRVLNLIEKEG"
     gene            3011..4213
                     /locus_tag="PLO58_08005"
     CDS             3011..4213
                     /locus_tag="PLO58_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HQO74771.1"
                     /translation="MGGFRKFLDQAGKPFRPGGKLAGLYYLFEAIDAFFYTPGKVTKG
                     TVHVRDFMDLKRMMITVFVALLPAVFMAFYNTGLQANLVLQSLGSGANFTWQQRLMLA
                     LGLDFNPESFWSNFIHGGLYFLPVYIITLIVGGFWEVLFAVIRKHEVAEGFFVTSLLF
                     PLILPPTIPYWQVALGISFGVVLGKEVFGGVGFNVFNPALVARAFLFFAYPAQITGDK
                     VWIAVDGISRATPLAQFAETSSAVTTSWLDAFLGFIPGSMGETSTLACLIGALILLVT
                     GVGSWRIMLSILIGMVTLSTTFNLMGSATNPMFAVTPLWHLVLGGFAFGTVFMATDPV
                     SGAVTKPGQWVYGLLIGVMVIMVRVLNPAFPEGMMLAILFGNMFAPLIDRIFINAHIK
                     RRATRDVR"
     gene            4203..5003
                     /locus_tag="PLO58_08010"
     CDS             4203..5003
                     /locus_tag="PLO58_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HQO74772.1"
                     /translation="MSDESARKTFLVALGVCLICAVMVATSVVLLRPRQQVNQELDKL
                     KNILQAANLYTDDAAVKKTFQEKIRPLLVELATGQIISEPPADKRLDPARFDSKAIAR
                     DPALSRTIPSADDLAHIGRQPKYMVVYEVVGNDQAGGVILPVYGKGLWSTLYGFIAFG
                     PDLQTVTGFIIYEHGETPGLGGEVDNPLWRAKWVGKRAFDDSGALKIEVIKGKVDPTS
                     PQSQYQIDGLSGATLTTRGIDQLVKFWLGENGYGPFLRNLQAKRMRNE"
     gene            4996..5613
                     /locus_tag="PLO58_08015"
     CDS             4996..5613
                     /locus_tag="PLO58_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HQO74773.1"
                     /translation="MNKSLTTLTTPIIKENPVIVQVLGICSALAVTSKLETAIVMALA
                     VTFVTAFANMAVSLIRRQVPGSIRIIVEMTIIASLVIVADQLIKAFAFGVSKQLSVYV
                     GLIITNCILLGRTEAFGLKNAPLASFLDGLGNGLGYSLILLIVASIRELFGAGKLLGF
                     TILRVNTEGGWYVPNGLLVLPASAFFLIGLIIWGLHTLKPETKQE"
     gene            5617..6225
                     /gene="nqrE"
                     /locus_tag="PLO58_08020"
     CDS             5617..6225
                     /gene="nqrE"
                     /locus_tag="PLO58_08020"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HQO74774.1"
                     /translation="MLEHYLSLFIKSVFIENMALAFFLGMCTFLAVSKRLETAVGLGI
                     AVVVVQTITVPINNLIYHNLLAEGALSWLGIDLDLTFLGLITYIGVIAAVVQILEMVL
                     DKFVPKLYHSLGIFLPLITVNCVILAGSLFMVERDYTFGESVVFGIGSGIGWALAIVA
                     LAGIREKLKYSNVPSGLRDLGITFITAGLMAMAFMLFSGIQL"
     gene            6239..7468
                     /gene="nqrF"
                     /locus_tag="PLO58_08025"
     CDS             6239..7468
                     /gene="nqrF"
                     /locus_tag="PLO58_08025"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HQO74775.1"
                     /translation="MSSLTLILIAVGVFVIIVLVLVGLILLAKSYLVPVGTIKININD
                     DPDYSFDAPVGGKLLNTLAEKEIYLPSACGAAGTCGLCKVQVLKGGGTILPTELAHIN
                     RREARAGVRLACQLAVKEDLWLSIPTEIFAIRKWICTVRSNRNVATFIKELVLDLPPG
                     EQINFRAGGYIQVETGPHHVNFKDFVIEEPFKSEWDRCNMWRYVSIVDEEVVRAYSIA
                     NYPDEKGIIMLNVRIATPPPEKPDAPPGKMSSYLFSLKAGDRVTISGPYGEFFAKETD
                     REMVFIGGGAGMAPMRSHIFDQLKRLHTQRKISFWYGARSLKELFYQEDFDALQREFP
                     NFEWHVALSEPLPEDNWKGYCGFIHEVVLNNYLINHPAPEDCEYYLCGPPLMVSAVLK
                     MLDDLGVEPENILYDEF"
     gene            7522..7692
                     /locus_tag="PLO58_08030"
     CDS             7522..7692
                     /locus_tag="PLO58_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74776.1"
                     /translation="MLIYFLLILGCTTLAFALMMAALKFSRYKGEKHSGQCGAECHCS
                     YDKKRTCVKPNR"
     gene            7901..>8829
                     /locus_tag="PLO58_08035"
     CDS             7901..>8829
                     /locus_tag="PLO58_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74777.1"
                     /translation="MISLTLLLGLCFSNALAVKFSWSELISDTLYISTYADALTLTGF
                     PAIPVNDERAESPTILGIQQIKKWHYIPVDQYIALNEPLEIQMSKFFQKFQVTGNQTL
                     VIDHLSLWYDASPAFGAARTLNGYTRLVDSSGETVRDWQWELREKTAKNQKPADNIAI
                     LLNKWMIAQTESLQRRPASDLPVSPYRYRRQMIPWTDIISLGDGYIVIAHLALEYPAD
                     QMVKFIRGSPGIYYRKSSKHESIAIGGRDQQWYFRLNRSWLARLNTTVRVGFNNFNPR
                     EYDYVDFWNIMLINLGFNASLEYRPIYHKGFFVG"
BASE COUNT         2160 a         1988 c         2250 g         2431 t
ORIGIN      
        1 gtttagcctg tcaatgtcag gtgacggaac cattagttat cgagctcgcc cagctggaaa
       61 tcccgatcct ttctgatgtc acccggataa aactcgtcgg agaaagcggc ttggcggtgg
      121 tggtagatct cggaacgacc actctggtag cccaattgct tgatttatcc agcggaatag
      181 ttctgggagt ggaaacggca ctcaatccgc aagcgcgcca tggcgccgat ataatgagtc
      241 gcatccagtt cggcttgaca accgcaggcc gagaattatt acaaaacctg attcgtcatc
      301 aaatcggaca gatgattact cgcctggtag aaaaatgtcg ctgtccggag cggcgactga
      361 aacagattaa tatcgtcggt aattccgtta tgcaccactt gtttggcggg ttgtcggttg
      421 aaccgctggc gctattgccg tttgaaatcg agcaacctgg tgaattggaa tggtcggcag
      481 aggaaatcgg ctggcagttg ccgggcaatc ccaaagtaaa atttctacct tgtctcggcg
      541 gattcgtggg gagtgacatt ctagctggaa ttctggcggt tggaatgcac cgcagtcccg
      601 aaaccgtcgc tctgattgat ctcggtacca acggcgaaat tgtcattggt aatcgtgaca
      661 gaattctgtg tgcttcgact gcggctggac cagcgtttga aggtgctcag attacgatgg
      721 gtatgcgcgc caccaccggc gccatctgga aagtttggct taccaatggc cagttgcaag
      781 ttgaagtaat cggcaatgtc ccggcgcgcg ggatttgcgg aagtggacta gtggacgcgg
      841 ttgccgccgc actcgatctt ggctggattg atccaaccgg acgaattacc ttgcccaaac
      901 gagaaatccc gctccagaag ccggtcgtca ttacccagtc cgatattcgt gaattgcaat
      961 tggctaaagg cgccatcgcg gctggaatcc agattttacg gaaacaaatg caattgaaac
     1021 cggctgatat aaagcaagtt tttctggcag gtgcattcgg caattacatc aatgtcgtca
     1081 gcgcgcggcg aatcggtctg tttgattttc ccacagaatt aattgaacaa gccggaaata
     1141 ccgctctgca cggcgccaag ttgtctttac ttaatgagca agagtaccac tcgattccgc
     1201 ttattacaaa acactttcca ctagctaccg acgctcaatt tatggaaata tttgccgaga
     1261 atatgaactt tccggggtga gccacgattt ccagttgccg gttacaattc accgaattcc
     1321 tgcgtccccg cttccaacta agcaaaataa tcaaccgcca ggaaaaccaa gatattaagc
     1381 tctcctaagt gcttataaga gattaacggc gatttgccgt cccattaaaa caatagaact
     1441 taacagcact tctctgccaa caagcaccca gctcattctc tataaaattc aagaaattct
     1501 gctaaagttt aaatttcact acataaaagt aatttttgtg tttgttcaat aaagtaattt
     1561 tttttaatct acgacttatg aaaattcgac taagatttag ttcagtataa atacaacaga
     1621 catttaatta taccaatcgt atcgggaaaa aaagcatacc gctaatgtta attaaaatta
     1681 aaaagggatt aaatctgcca attgccgggg caccggagca ggttatttac ccggctcaat
     1741 cggtctcgca ggtagcgctt ttaggactgg attacgtcgg gctgaagccg caattcgcgg
     1801 taacgatcgg cgatcaggtg aaactcgggc aacccctttt tagcgataaa acttacccgc
     1861 agattcgatt caccgcgccg ggtgccgggc aagttattgc cattaatcgt ggcaccaagc
     1921 gggcttttct atcgctggta atcaaattgg atggcgatga tgctgttgaa ttcaaatctt
     1981 tttcagagca acaaattggt tctctaacgc aagaagaaat tataaatcaa ttgctggagt
     2041 ccggtttatg gccctcgttc aaagccagac ctttcggtaa attagctaat ccggaaagtc
     2101 aaccacattc gattttcgtt acggctatgg acactaatcc actggcgcca gacgttgcgg
     2161 tcatactacg tgaacagtcg aatgatttcc agcgcggctt agcactcatt gccaaactga
     2221 ctgctggaaa agtgttcgtc tgccaagcac cggatacagc gattcccgtt cctgaaattg
     2281 aaaacataga aatagtggaa tttgccggac cgcatcccgc cggactggca ggaacgcata
     2341 tccatcttct cgacccggtt tcgctccaca aaacagtttg gagcattggt gctcaggatg
     2401 tcgcggctat cgggaaattg tttactaccg gcagattgcc aaccgcgcga atcgtcgctt
     2461 tggcgggttc agcggttaaa aagcctcgac tgattaccac gcgagtcggc gcaaacctaa
     2521 gcgaattaac cgacggcgaa ctggcagaag gccataatcg cataatttcc ggttcggttc
     2581 ttgccggaca tacagcggca ggcgagttga attttctcgg acggtttcac caaattgtga
     2641 gtgtcatccc ggaaggtggc cgtcgtaaat tattcggctg gcttagtccc ggttggcggc
     2701 tattttcggt gaagaatatt gcgctggcgg cttttttacc aaaccgtaag ttggtcttcg
     2761 acaccgatac gcacggtagt gaacgcgcga tcgttcccat cggtagttac gaaaaggtaa
     2821 tgccattgga cattttgccg acttaccttc tgcgagcgtt ggcggtagat gacctcgaag
     2881 aagccgaaaa actcggttgt ttagaactgg ttgaggaaga tttggcgtta tgtacatttg
     2941 tctgtccctc gaaaattgac catggtcaga atctgcggcg cgtgttgaat ttaatcgaga
     3001 aggaaggata gtgggcgggt tccggaaatt tctcgatcag gcaggcaagc cttttcgacc
     3061 cggcgggaag cttgctggat tatattacct gttcgaagcg attgacgcct ttttctatac
     3121 gccgggcaaa gttaccaaag gcacggtgca cgtacgtgat tttatggatt tgaaacggat
     3181 gatgatcacg gttttcgtgg cgctgttgcc ggcagttttc atggcttttt ataataccgg
     3241 tctgcaggct aatctcgtat tgcagtcgct cggctccggc gctaatttta cctggcaaca
     3301 acgcctgatg ctggcattgg gactggactt caatccggaa agtttctggt ccaatttcat
     3361 ccatggcggt ttatatttcc tgccggttta tatcataacg ctgatagttg gcggattctg
     3421 ggaagtgctg ttcgccgtaa ttcgcaaaca tgaagtcgcg gaaggttttt ttgttaccag
     3481 cctgcttttt ccgctgatcc tgccgccgac gattccctac tggcaggtag cattgggaat
     3541 ttcattcggt gtagtgctcg gcaaggaagt attcggcggg gtcggcttca atgttttcaa
     3601 cccggcattg gtggcgcgtg cttttctgtt ttttgcttat ccggcgcaga ttaccggtga
     3661 caaggtttgg atcgcagtgg acggcattag ccgggcgacg ccgctggcgc agttcgcgga
     3721 aacgtcgtcg gcagttacta ccagctggtt ggatgctttc ctggggttta ttcccggttc
     3781 aatgggcgaa acctcgactt tagcctgttt aattggggcg ctaattttat tggttaccgg
     3841 agtaggtagt tggcgcatta tgctgagtat tttaatcgga atggtgacac tttctactac
     3901 tttcaattta atgggcagtg ccactaatcc aatgttcgcg gtgacgccgc tctggcatct
     3961 ggtgctgggc ggatttgctt ttgggacagt ttttatggcg accgatccag tttcgggcgc
     4021 ggttaccaaa ccgggccaat gggtatatgg tttactgatt ggcgttatgg ttattatggt
     4081 gcgcgttttg aatccggctt ttccggaagg tatgatgctg gcgattttat tcggaaatat
     4141 gttcgcgccg ctaatagatc gcatttttat taacgctcat ataaagagga gagcaacccg
     4201 cgatgtcaga tgaaagcgcg cggaaaacgt tcttagtagc actgggcgtt tgcctaattt
     4261 gcgcggtgat ggtggcgact tctgtggttc tcctgcgtcc gcgccagcaa gtaaaccaag
     4321 aattggacaa gttgaaaaat attctccaag ccgccaacct ttacaccgat gacgccgccg
     4381 tgaaaaagac ttttcaggag aaaattcgcc cgctactggt tgagcttgcc accggtcaaa
     4441 ttattagtga acctccggcg gataagcgcc tcgatccggc acgctttgat tccaaagcca
     4501 ttgctcgtga cccggcctta agtcggacaa ttccatctgc cgatgatttg gcacacattg
     4561 gtcgtcagcc aaaatatatg gtagtttatg aggttgttgg aaatgatcaa gctggtggtg
     4621 tgattctgcc ggtttacggc aaaggtctct ggtccacgct ctatggtttc atagcttttg
     4681 gtcctgattt gcagaccgtt acgggtttta tcatttacga acatggagag acacccggtc
     4741 taggtggcga agttgataat ccgctctggc gcgccaaatg ggttggcaaa cgcgctttcg
     4801 acgattctgg cgcgctaaaa atcgaagtta tcaaaggtaa agttgaccca accagtccgc
     4861 agtcccaata ccagattgat ggcttgtccg gagcaacgct caccacgcgc ggcattgatc
     4921 aactcgtaaa attctggctc ggagaaaatg gctatgggcc gtttttgcgc aatcttcaag
     4981 ccaagaggat gagaaatgaa taagtcactc accactttaa ccacgccgat aattaaagaa
     5041 aacccggtga tcgttcaggt gctgggaatt tgttcggcgc tggcggttac ttctaaattg
     5101 gagacggcga tcgtaatggc attggcggta actttcgtta cggcttttgc gaatatggcg
     5161 gtcagtttga tccggcgcca ggttcctgga agtatccgca ttatcgtcga aatgacgatc
     5221 atcgcctcgt tagtgattgt cgccgatcaa ttgattaagg ctttcgcttt cggcgtcagt
     5281 aagcaactct cggtttatgt tggcctgatc atcactaact gcattctgct ggggcgcacg
     5341 gaagccttcg gtttaaaaaa tgcgccgtta gccagtttcc tggatgggtt aggaaatggg
     5401 ttgggttata gtctgattct tttgattgtg gcttcgattc gcgaactatt cggcgccgga
     5461 aaattgctcg gctttacgat tctgcgggtc aataccgaag gcggctggta cgtgccaaat
     5521 ggattattgg ttttgcccgc cagcgctttt ttcctgatag ggttaattat ctggggttta
     5581 cacactttga aaccggaaac aaagcaggag taaagtatgc tggaacatta tctgagcctg
     5641 ttcatcaagt ccgttttcat cgagaatatg gcattggctt ttttcctcgg tatgtgcacc
     5701 tttttggcgg tctccaaacg tttagaaacg gcggtcgggc tgggtattgc ggtcgtcgtg
     5761 gttcagacga tcacggtccc tattaataac ctaatctatc acaatttgct ggctgaaggc
     5821 gcgttgagct ggttgggcat tgacctagat ttgacgtttc tcggtttgat aacttatatc
     5881 ggcgtcatcg ccgccgttgt ccagattctg gaaatggtgc tggataaatt tgtaccaaag
     5941 ctttaccatt cactcgggat ttttctgccg ctaattaccg ttaattgtgt gattctggct
     6001 ggctcgcttt ttatggtcga acgcgattac accttcggtg aaagtgtggt ttttgggatt
     6061 ggttcgggaa taggctgggc actagcgatt gttgctttag ccggtattcg cgagaaattg
     6121 aaatatagta acgttccatc cggtctgcgt gatctgggca ttacttttat tacagccgga
     6181 ttaatggcga tggcttttat gcttttttcc ggaattcagc tttagtagat acgattttat
     6241 gagcagtttg actttaatct taattgcggt cggagttttt gtcataatcg tgctggtgct
     6301 ggtcggcttg atcctgctgg caaagtccta tttagttccg gtcggcacga ttaaaattaa
     6361 tataaatgac gacccggatt attccttcga cgcgccagtc ggcggcaaac tattaaatac
     6421 cttggctgaa aaggaaatat atttgccgtc cgcctgcgga gccgctggca cctgcggact
     6481 ctgtaaagta caagttttaa aaggcggcgg cacgatactg ccgacggagc tggcgcatat
     6541 caatcgccgg gaagcccgcg caggtgtgcg gcttgcctgt caactggcgg tcaaggaaga
     6601 tttgtggctc tcgattccaa cggaaatttt cgccattcgc aaatggattt gcacggtgcg
     6661 ctcgaatcgc aacgtggcga cttttatcaa agaactcgtt ctagatttgc caccgggcga
     6721 gcagataaat ttccgggcgg ggggttacat tcaggtggag accggaccgc atcatgtcaa
     6781 tttcaaggat tttgtaattg aggaaccttt taaatcggaa tgggatcgct gtaatatgtg
     6841 gcgctacgtt tccattgtgg acgaagaggt tgtgcgagct tattcaatcg ctaattatcc
     6901 ggacgaaaaa ggcataatta tgctgaatgt gcgaatcgcc acgccgccgc cagaaaagcc
     6961 ggatgcgcct ccaggtaaaa tgtcgtccta tttattcagt ttgaaggcgg gcgaccgggt
     7021 gacaatttct ggaccctatg gagagttctt cgccaaagaa accgatagag aaatggtctt
     7081 catcggcggc ggagccggaa tggcgccgat gcgttcgcat attttcgatc aattgaagcg
     7141 cctgcacacc cagcgtaaaa tctccttctg gtacggcgcc cgtagtctga aggaactgtt
     7201 ttatcaggag gacttcgacg ctctgcagag agaatttccg aattttgaat ggcacgtagc
     7261 gctttccgaa cctttgccgg aggataattg gaaaggctat tgtggattta tccacgaagt
     7321 ggttttgaat aattatttga tcaaccaccc ggcgccggaa gactgcgaat attacctttg
     7381 cgggccgccc ttaatggtca gcgccgtatt gaaaatgctg gacgatctgg gcgttgaacc
     7441 cgagaatatt ttgtacgatg aattctgatg aactaaaatt gtgaaaccgg gacgagattc
     7501 aagcaaaaac aggtaaagtt gatgctgatt tattttctat taatattggg atgcacgaca
     7561 ttagcttttg ccctaatgat ggcggcgctg aaatttagtc gctataaggg cgaaaagcat
     7621 agtggacaat gcggtgcgga atgccattgt tcgtatgata aaaagcggac ttgtgttaag
     7681 ccgaatcggt aatcattaat ttcgcggcag aaacctcttc ctttcagctt cacacagttc
     7741 aggcggaata ggcagtgtca gaaaaaaggg gaaaaatctt ttaatcgcgc ctaaaaactc
     7801 catcgctctt aaagttgcat atttttggcg ttggtcgtaa aatttacacc aaccttaatg
     7861 aaccaattac ttgacgttga ataatcaaaa tatcgcaaaa ttgatctcct tgactttatt
     7921 gttggggctt tgtttttcga atgccttagc agttaaattt tcctggtcgg agctaatttc
     7981 cgatacgctt tatattagca cctatgccga tgcgcttacg ctgacgggat ttccagctat
     8041 cccggtaaac gatgaacgag ccgaatcacc gacaatcctc ggcattcagc aaatcaagaa
     8101 atggcactac attccggttg accagtacat cgccttgaat gaaccactgg aaatacaaat
     8161 gtccaagttt ttccaaaaat ttcaggttac gggtaatcaa actctggtta ttgatcatct
     8221 cagtctctgg tacgacgcca gtccagcatt cggcgcggcg cgcaccttga acggctatac
     8281 gcggctggta gattccagtg gagagaccgt gcgcgattgg caatgggaat tgcgcgagaa
     8341 gacggctaag aatcaaaagc cggcagataa tatcgccatt ctactcaata aatggatgat
     8401 tgcccagacc gaatctttgc aaagacgacc agcctccgat ttaccagtat cgccttatcg
     8461 ttaccggcgg cagatgatcc cttggactga tataattagt ttaggcgacg gctatatcgt
     8521 aattgctcat ctggcgctgg aatatccagc cgatcagatg gtaaagttca ttcgcggttc
     8581 gccgggaata tattaccgca aatcttcgaa acacgaatcc atcgctatcg gcggcaggga
     8641 tcagcagtgg tattttcgcc tgaatcgttc ctggttggcg cgtctcaata ccactgttcg
     8701 ggttggtttc aacaatttca atcctcgcga atatgactat gttgatttct ggaacattat
     8761 gttgatcaat ttagggttca atgccagcct tgaatatcgg ccgatttatc acaaaggatt
     8821 tttcgtagg
//
LOCUS       DAOMJU010000088         8826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_8308_length_8826_cov_17.795348, whole genome shotgun sequence.
ACCESSION   DAOMJU010000088 DAOMJU010000000
VERSION     DAOMJU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8308_length_8826_cov_17.795348"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLO58_08040"
     CDS             complement(<1..494)
                     /locus_tag="PLO58_08040"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQO74778.1"
                     /translation="MGKAKIFKKEWLIVGVIILTMKSIASPASTVRGVVTDSLTSKTL
                     VGANVFLVGTALGSATDIEGNYRIESVPAGNYILRVTYIGYKNKEYNVVVPEDGTVIQ
                     DVKLSPDVIRGQEVIVRGQAIGQAAAINQQITSATIINVVSEEKIQELPDANAAEAIG
                     RLPGV"
     gene            724..1959
                     /locus_tag="PLO58_08045"
     CDS             724..1959
                     /locus_tag="PLO58_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74779.1"
                     /translation="MLKYQLMINEQTRKKLLEKILQSKEFSTSKIYASYLTYLVEAAE
                     RGKKLKEISIAIEFFGKDANFNPAEDTIVRTHTYNLRKKLQNYYYDEGKDDKYRLRIP
                     KGQYDVTFIPALENTYHPKRILRFMVREYKITLIVALLILLIIAIIYNFNLQNQLTSY
                     RVIDKKDPIWSEYLQSKQPVLIVLGNHFFFDDYSEKFRSNITIRHGRVNSYEDFNALQ
                     SQFPENVLKPSDEPYFPYHSIWSLPPVLSILNSVGQKPILRRSSLITPQTLGEYNIIF
                     LGSIKTLYILKHTLTQSHFSFEILPHIITYTNGDSCRTFQTNLHSTGLNEDLVLAVKL
                     PGPADNSIFIIASYHSLGAPEVVNQLTQTDSRKQIEQKFIEKYGYMPRYFETLFRVNG
                     IDKTAYSTEMLIFNEITKD"
     assembly_gap    1966..2065
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2079..4247)
                     /locus_tag="PLO58_08050"
     CDS             complement(2079..4247)
                     /locus_tag="PLO58_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQO74780.1"
                     /translation="MISNLKNIKFSKILLIICLIFNSRLLADDKPKTALVLSGGGARC
                     FAQIGVIKALEEIGFYPDFIVGTSFGALIGALYAGGSTPQEIEEFIANTRWNRVLASQ
                     PFRDIEFVSQKIRTLPELFTLRFDENFNVIFPRNLLSTQALQERIFEMTIYPEYYAGS
                     DFDSLAIPLRIIATDLKTGKMVVLSNGNLARSVTASSAFPIILAPVKYGTYLLADGGL
                     TDNVPIDVALDLGAEFIVAVDVSSKIVPLAENFDVFDIFGQAMNTLAYPSDTKNLELA
                     DVLIRPEIDHITSTDFDSLNSLVESGYRSCQKYWGKIQPYADNICKSPDFLENAINKL
                     NNTSINQIKYSSDQATREYILRREMELKEGDRWNLKLAQRSMKNLYSTGLFKNVYLSL
                     SEVDSSRADLLVEVEEHERTAFSFGARYDSERKASAFISGKYRNLLGRGIDNQVNLIV
                     SDLLFRAEWNSRTIRVLSSNFTGYTSLYHLNESIPLYNNDGRRVESGKLYRTGLEANA
                     GIQIKRVGLTAFGLKWENTVADSTIHYIPRVNRDNYSVGSLTLRILVDNVDDPDLPKS
                     GRVNDIRYEHSIERDRLKQFDRISVESVTYETFDEKYTFSTTLHLGYLNKVLNHYEQF
                     RLGGLNSLPGYHQDELWGSMIGVLGLGCRAPLTSGIYCRLSGMVGNVWDGFSDFDWRQ
                     SRAGISLGIVIPTPIGPISADYGIGSHNHRLFYISVGRYF"
     gene            complement(4296..4973)
                     /locus_tag="PLO58_08055"
     CDS             complement(4296..4973)
                     /locus_tag="PLO58_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HQO74781.1"
                     /translation="MLGTLVNVGMVLLGSSLGLLLHTRLPRKFTVIVFQVIGLFTLFL
                     GFSMALKTTNFLIMVFSGALGAVIGELCKIEEAINNLGENLKRRLKSDNDKFAEGLVT
                     AFLMFCMGSMTILGAFEEGMGGEPNLLLAKSLLDGFSSMILSAGLGVGVLLSVIPLFL
                     YQGGLTLLAGLLGDVLNPTVINEMTAVGGLLLIGLGLTILEIKTIKVVNMLPGLVVAI
                     ILAGIFL"
     gene            complement(5041..5244)
                     /locus_tag="PLO58_08060"
     CDS             complement(5041..5244)
                     /locus_tag="PLO58_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HQO74782.1"
                     /translation="MKKIYRSRTDQKLAGICGGLGEIFNIDSNLIRLALVFATILTGF
                     FPLLITYIVGWIIIPEGPVEGKG"
     gene            complement(5515..6588)
                     /gene="rseP"
                     /locus_tag="PLO58_08065"
     CDS             complement(5515..6588)
                     /gene="rseP"
                     /locus_tag="PLO58_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQO74783.1"
                     /translation="MLYTILAVLIVIGGVVIAHELGHFVFAKLTGMRVEVFSIGFPPK
                     IISKKIGETEYQLSAIPLGGYVKVSGVVDESFDTTAAESNEPWTFAGKKTWQKLLFIS
                     GGVIFNVLFAFVIFSGLTMHEGIYEPVDKPIVESVAPGLPADSLGIKPGDIILAINGT
                     AVESWSSMTELIHSHPYDSILIKWQSGGTIYEKKVKPAANKILKGSELVEVGLIGISP
                     TMNHRPAKFFESLDNGMQNTWYWLKITVISLKMLITGEESIKNIGGPIFIAQLAGETA
                     KFGFGSLLNLMAIISLNLALINILPVPAFDGGHLLITLIEAIKRRPLSVNAKVRIQQI
                     GIAIILILTVLVLFNDISRILKW"
     gene            complement(6607..7773)
                     /locus_tag="PLO58_08070"
     CDS             complement(6607..7773)
                     /locus_tag="PLO58_08070"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQO74784.1"
                     /translation="MAEAGKIRAISVLGSTGSIGVNALKVISASRDRFTVNYLTAGRN
                     GTLLIEQAKKFHPKAVAIADESQYRLLKENLSGTNIEILVGKDGILEIAGRRDVDLVV
                     NAIVGMAGLEPTYRALTAGRNVALSNKESLVMAGEIIMNLIAQSGLNLFPIDSEHSAI
                     WQCLAGEDKVSVKRLILTGSGGPFRARPQAEFKSITPAEALRHPTWTMGRKITIDSAT
                     MMNKGLEIIEAFWLFGVEPEQIEVLIHPQSVIHSMVEFCDGSIKAQLGLPDMRLPIQY
                     AIGYPERLPIIWEKIEWAKLGPLTFEVPDPVKFPALRLAFEALKRGGTAPAIMNVINE
                     LAVYAFLDEKIGFMEITDIIEKALNEIPVIDKPNIEDILNVEPMAREFFGNFIR"
     gene            complement(7775..8506)
                     /locus_tag="PLO58_08075"
     CDS             complement(7775..8506)
                     /locus_tag="PLO58_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74785.1"
                     /translation="MNIRQFQKFKYLRFVGLFLIAANLSANELDLTALPKNQVRGELQ
                     NILPKLCAGLELPCILLSRPFTPGVLISARYNCPDLLASPSNLTGLPTCGMAVGATPD
                     LIIAGQIGTGRWQEETLNSMGIYLAYVWGKPTAPNQIIGGVNHTKGPVDFHFLDMSLG
                     YLKLFQFNQWLFSVAGTAHFTQVGIHVTDNLNSADNYKARKNIDFMLVNITLQRNLGE
                     HFQIGTNLTFSPKSVSGGIAIGSYF"
     gene            complement(8509..>8826)
                     /locus_tag="PLO58_08080"
     CDS             complement(8509..>8826)
                     /locus_tag="PLO58_08080"
                     /inference="COORDINATES: protein motif:HMM:NF025097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HQO74786.1"
                     /translation="GVQQFSRIGQHSIIGGHYRVPKDVPPYIMAAREPLRYEGLNLVG
                     LKRRNFPPETILALKRAYTLIYNSEYNVSDAIKKIEETPELMIPEVRNVIEFIKKSSR
                     GII"
BASE COUNT         2704 a         2080 c         1566 g         2376 t
ORIGIN      
        1 acgcctggta atctgccaat agcctcggcg gcattggcgt cgggtaattc ctgaatcttt
       61 tcctccgata cgacgttgat tattgtcgcc gatgtaatct gctgattgat ggctgccgct
      121 tgacctattg cttgcccccg aacgattact tcctgccccc gaatcacatc ggggctcaat
      181 ttcacgtcct ggataaccgt cccgtcttcg ggaactacaa cgttgtattc tttattctta
      241 taaccgatat atgtaactcg taatatataa ttgccagctg gcaccgactc gatgcgataa
      301 ttaccctcga tatccgtggc actccccaga gccgttccta ccagaaatac attggctccg
      361 accaaagttt tagatgtcag cgaatccgta acaactccgc gaactgtact ggcgggactg
      421 gcaatacttt tcatcgtaag aataattacc cctacaatca accattcctt tttaaatatt
      481 ttggctttcc ccataaaatt tcaccttata taattaaact tattcgccta cttctactat
      541 ttcactgttt tggtcaataa tgcctgtcaa attcaaactt tctaaaacat ttttcaagta
      601 aagtcaaata acacttgaaa tagaacgatt atttcattaa ctataaagat aaaaactgtg
      661 aataaccttt tttaacattg ttaaacatgt catacaactt tttcttggct tttgttaaaa
      721 atattgttaa agtatcaatt gatgattaat gagcaaaccc gcaagaaatt attagaaaaa
      781 attctgcaga gtaaggagtt tagcacttct aagatttacg cttcttactt gacatacctg
      841 gttgaagcgg ctgaaagggg gaaaaaatta aaagaaattt cgatcgcaat cgaatttttc
      901 ggcaaagatg ccaattttaa tccagccgaa gataccatcg tccgtaccca tacttataac
      961 ctgcgcaaga aattgcaaaa ttattactac gatgaaggca aggacgacaa atatcgtctg
     1021 cgaattccca aaggccaata cgatgtcact ttcattccgg ctttagaaaa tacctaccac
     1081 cccaagcgaa tcttgcgctt tatggttcgt gaatacaaaa taaccctaat cgtcgcacta
     1141 ctaattttat taataatcgc aataatttat aattttaacc ttcaaaacca gcttacgtcc
     1201 taccgggtca ttgacaaaaa agatccaatc tggagcgagt atctccaatc caaacaaccc
     1261 gtactgattg ttttgggcaa ccattttttc tttgacgatt acagtgaaaa attcagaagc
     1321 aatataacca ttcggcatgg acgcgtcaat tcatacgaag attttaatgc tctccaaagc
     1381 cagtttcccg aaaatgtact caaaccttcc gatgaaccct atttccccta tcatagtatc
     1441 tggagtttgc cgcccgttct ctcaatcttg aactctgtcg gacaaaaacc aattttaaga
     1501 agatcatcct tgattactcc tcaaacatta ggcgaataca atataatttt cctcggcagt
     1561 attaagacac tttatatcct caaacacact ttgactcaat cgcactttag tttcgagatt
     1621 ctgcctcaca tcatcactta caccaacggt gattcttgtc gcaccttcca aaccaattta
     1681 cattccaccg gattgaacga agaccttgtg ttagcggtta aactacctgg cccagctgat
     1741 aattccattt ttatcatcgc ttcctaccat tccttaggag cacccgaagt cgttaatcaa
     1801 cttactcaga ctgattcccg caaacaaatt gaacaaaaat ttatcgagaa atacggctac
     1861 atgccgcgct atttcgagac tttgtttcgg gtaaatggaa tagacaaaac cgcttacagc
     1921 actgagatgc tgatcttcaa cgaaatcacc aaagattaaa cctcannnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnntgctt tgagatcatt aaaagtaacg tccgactgaa
     2101 atatagaaaa gccggtgatt atgagaacca atgccataat cagccgaaat cggtccaatc
     2161 ggtgtcggta taacaatccc aagactgata cctgctcgag attgtctcca gtcaaaatca
     2221 ctgaatccat cccatacatt gccaaccatt ccggacagac gacaatagat accggaagtt
     2281 aaaggcgccc tacatcccag tccgagcaca cctatcatcg aaccccataa ttcatcctga
     2341 tgataaccag gcaacgaatt taatccacca agtcggaatt gctcgtaatg attcagcact
     2401 ttattcaaat aaccaagatg aagagtagtc gaaaaagtat atttttcatc gaaagtttca
     2461 taggtcactg attcgaccga tatccgatcg aattgcttta agcgatctcg ttcgattgaa
     2521 tgttcatacc gaatatcatt tactcgaccg gatttaggta aatcgggatc atcaacatta
     2581 tcaactaata tccgcagagt taaactgcca accgagtagt tatcacgatt aacgcgggga
     2641 atataatgga tggtagaatc ggcaacagta ttttcccatt tcagaccaaa ggcggttagt
     2701 ccgacccgtt tgatctggat tccggcatta gcctccagtc cggttcggta taatttaccg
     2761 gattcgacgc gtcgcccatc attattataa agcgggatac tttcattcaa atgatacaaa
     2821 gaagtataac cggtaaaatt cgaactaagc acacgaatag ttcgggaatt ccattcagcc
     2881 cgaaacaaaa ggtcactgac aatcaaatta acctgattgt caatgcctct gcccagtaaa
     2941 tttcgatact ttccggaaat aaaggcactc gctttacgct cgctatcata acgcgccccg
     3001 aaactgaatg ccgtgcgttc gtgttcttca acttcgacta ataaatccgc tcgactggaa
     3061 tcaacctcag acaaagatag gtaaacattt ttaaataatc cggtactata caggtttttc
     3121 atactgcgtt gtgccagttt taaattccac cggtcacctt cttttaactc catttcacgc
     3181 cgtaaaatgt actcgcgggt tgcctgatcg ctggaatatt taatttggtt gattgaagta
     3241 ttatttaatt tatttatggc attttctaaa aaatccggtg atttacaaat attgtctgca
     3301 taaggttgta tttttcccca atatttctgg caactgcgat agccactctc gactagagaa
     3361 ttcagtgaat cgaaatcggt cgaagtaata tggtctatct ccggtcggat gagaacatca
     3421 gccaattcga gatttttcgt atcagaagga taggctaaag tattcatcgc ctgcccaaaa
     3481 atgtcaaata catcgaaatt ttccgccagc ggcactattt tactactaac gtccacagct
     3541 acaatgaatt cagcacccag atcgagtgcc acatcaatcg ggacattgtc ggtcaagcca
     3601 ccatcggcta aaagatatgt tccatacttg accggcgcta gaatgattgg aaaagccgaa
     3661 ctggcggtaa ccgaccgggc aagattgccg ttactcagaa caaccatttt gcccgttttt
     3721 agatcagtag cgataattcg taacgggatt gccagactat cgaaatccga cccagcataa
     3781 tactccggat agatcgtcat ttcaaagata cgttcttgta aagcttgcgt tgataacaaa
     3841 ttacggggaa atatcacatt gaagttctca tcaaaccgca aagtaaacaa ctctggtaaa
     3901 gtcctaatct tctgggatac aaactcaata tcgcgaaatg gctgagaagc caaaactcgg
     3961 ttccagcgcg tattagcaat aaattcctcg atttcttgag gcgtactccc accagcatac
     4021 aaagccccaa taagagcccc aaaacttgtc ccaacgataa aatcgggata aaagccgatt
     4081 tcctccagtg ctttaatcac cccaatttgg gcaaaacaac gtgccccgcc cccacttaac
     4141 accaacgcag ttttgggctt atcatcagct aaaagcctcg aattaaatat cagacagatt
     4201 ataagtaaga ttttactgaa cttaatgttt tttaaatttg aaatcattga ctgatataaa
     4261 actcattcga acggcgctga caatcagaaa atcaactaca gaaaaatgcc agccagaatt
     4321 attgctacaa ccagaccggg caacatatta accaccttga tggtcttaat ttccagaatc
     4381 gtcaatccga gaccgattag caataaaccc ccgacagccg tcatttcatt aatcacggtt
     4441 ggatttaaaa catcacccaa gagaccagct aaaagcgtca aaccaccttg ataaaggaac
     4501 aacgggatca cagataacaa aactccaact cccagaccgg cagacaaaat cattgaagaa
     4561 aatccatcca gcagtgattt cgctaataac aaattcggtt cgccacccat cccttcttca
     4621 aaagcgccta aaatagtcat tgaacccata cagaacatta gaaaggcagt aaccaaacct
     4681 tcagcgaatt tatcgttatc gctttttaaa cgacgcttga gattttcacc gagattattg
     4741 attgcctcct ctattttaca tagttcaccg atcaccgctc ccagcgcgcc actgaaaacc
     4801 ataatcaaaa aattagtagt tttcaacgcc atactgaaac cgagaaacaa ggtaaataac
     4861 ccgatcactt ggaagacaat cactgtaaat ttgcgcggca atcgggtgtg caacagtaat
     4921 cccagactac tgcccagtaa caccatcccg acatttacca aagtgcccag cataacgaca
     4981 tccgctccta atagtcgaga atcttcgaca ctcccgccta atcttagcct taactcagtc
     5041 ttaacctttc ccttctaccg gtccctccgg aataatgatc caccccacaa tataggtaat
     5101 caataatgga aaaaatccag ttagaatcgt ggcaaagacc aacgccagac gaattaggtt
     5161 cgagtcaatg ttaaaaattt cacctaaccc gccgcagatt ccggctaact tttggtcagt
     5221 tctcgaacga tagattttct tcataaacac ttctccgtta aaatcactcc aattttattg
     5281 attatttcgg aagttggaaa gcaggatttt taaataattg ggtaattgcg ggtgttttct
     5341 aatcattatc aataaatatt catttgaatt tcgacgaata ttgacaaaat ccgtcagcct
     5401 gcgggagctt attcagcgta aaagccagct cggtttcagg attcaggcaa gtgcctttga
     5461 aaataggatg cggactgagc ttaattaccc gctactaacg acgaacttcc gtttctacca
     5521 cttaagaata cgggaaatat cgttaaaaag aaccagcacc gttaaaatta agataatcgc
     5581 tataccaatt tgctggatgc gcaccttggc gttaaccgaa agtggacggc gtttaatagc
     5641 ttcaattaaa gtaatcagaa gatgcccgcc gtcaaaagcc ggtactggta aaatgttaat
     5701 aagtgccaaa ttgagactta taatcgccat caggtttaga agggaaccaa atccgaattt
     5761 agcggtctct ccagctaatt gggcaatgaa aatcggtccg ccgatattct ttatagattc
     5821 ttcgccagta atgagcattt tcaaggaaat tacagttatc ttaagccagt accaggtatt
     5881 ttgcatacca ttatcaagcg attcgaaaaa ttttgccggg cggtgattca tcgtcggcga
     5941 aattccaatc agaccaactt cgactaattc agaacccttc aatattttat tagcggctgg
     6001 ctttaccttc ttttcgtaaa tcgtgccgcc actctgccat ttaattaaaa tagaatcata
     6061 gggatgacta tgaatcaact cggtcatact gctccaagat tctaccgctg tgccattaat
     6121 cgcaaggatt atatcaccgg gtttgatccc caacgaatcc gccggtaaac caggagcaac
     6181 gctttccacg atcggtttat ctaccggttc ataaattccc tcgtgcatag tcaatccgct
     6241 gaaaatcacg aaagcaaaaa ggacattgaa aattactccg ccggaaataa aaagtagttt
     6301 ttgccaagtc tttttaccgg cgaatgtcca aggctcgttg ctttcggcgg cggtagtatc
     6361 aaaactctcg tcaactacac cggagacttt gacatagccg cccagaggaa tcgccgagag
     6421 ctggtattcc gtttcaccga ttttcttact aattattttg gggggaaaac cgattgaaaa
     6481 gacctccacc cgcataccgg tcaatttagc aaaaacaaaa tgtcccaatt cgtgcgcaat
     6541 aaccactccg ccaattacaa tcaaaaccgc taaaatagta taaagcatct ataactcttc
     6601 ctttctttac cttataaaat taccaaaaaa ttcccgtgcc attggttcaa catttaaaat
     6661 atcttctata ttcggcttat caataaccgg aatctcattc agcgcttttt caattatatc
     6721 agttatttcc ataaatccga ttttctcatc cagaaaagca taaaccgcta gttcattgat
     6781 cacattcata atcgccggcg ccgtgccgcc acgctttaaa gcctcaaaag caagccgtaa
     6841 tgccggaaat ttcacggggt ccggcacctc gaaagttaac ggacctagtt tagcccattc
     6901 aattttttcc cagataatcg gcagacgctc agggtaaccg atagcatatt gaatcggcaa
     6961 gcgcatatcg ggtaaaccca gttgggcttt gatcgaacca tcacaaaatt ccaccatcga
     7021 atgaattacc gattgcggat ggattaaaac ctcgatctgc tccggctcga cgccgaataa
     7081 ccaaaaagct tcgataatct caaggccttt attcatcatt gtcgctgaat caatagtaat
     7141 ttttctcccc atagtccagg tcggatggcg tagagcctca gccggagtaa tacttttgaa
     7201 ttccgcctga ggacgggcgc ggaacggccc accggatccg gtcaaaatca aacgcttaac
     7261 actgactttg tcctcaccgg ctaaacattg ccagatggca ctatgttcgc tatcaatcgg
     7321 gaaaagattc aagccgcttt gcgcaattag gttcataata atttcaccag ccataaccag
     7381 cgattcttta ttagaaagag caacgttacg accggcagtc aaagcccgat aggtcggttc
     7441 taaacctgcc atcccgacaa tagcattcac cactaaatca acatcccgac gaccggctat
     7501 ctccagtatg ccgtccttcc cgactaaaat ctcaatattc gtaccggaga ggttttcttt
     7561 taataatcgg tattgcgatt catctgcgat ggcgactgcc ttagggtgaa atttcttagc
     7621 ctgttcaata agtaaagtcc catttcgacc agcagtaagg taattaacag taaatctatc
     7681 ccggctcgca gaaataacct ttagagcatt gacgccgatc gaaccggtcg aacccagaac
     7741 tgagattgcc ctaattttac ctgcttccgc catcttagaa ataacttcct attgcgatac
     7801 cgcctgaaac acttttcggc gaaaaagtta gattggtacc aatttgaaaa tgctcaccca
     7861 agttacgctg aagagtaata tttaccagca taaaatcaat atttttccta gctttgtaat
     7921 tatcagccga attcaagttg tcagtaacgt gaattccgac ttgagtaaaa tgggctgtgc
     7981 cggcaacgga aaaaagccat tgattaaact gaaataattt taaatagccc agagacatat
     8041 ctaaaaaatg aaaatcaacc gggcctttgg tgtggttgac tccgccaata atctgattcg
     8101 gtgcagtcgg cttcccccag acataagcga ggtaaattcc catagaattt agcgtttcct
     8161 cctgccagcg gccagtacca atttgcccgg ctataataag atcgggagtg gcacctacag
     8221 ccatcccgca agttggcagt cccgtcagat ttgaaggtga agccagtaaa tccggacaat
     8281 tatagcgagc gctaattaat acacccgggg tgaaaggacg acttaaaaga atacaaggta
     8341 attctaatcc ggcacacaat ttgggcaaaa tattttgcaa ttcgccgcgc acctggtttt
     8401 tgggcagtgc tgtcagatct agctcattgg ctgataaatt ggcggcaatt agaaataatc
     8461 caacgaatcg taaatatttg aatttttgaa attgccgaat attcaatatc agataatgcc
     8521 acgtgagctt tttttaatga attcaatgac attgcgtact tccggaatca tcagttccgg
     8581 tgtttcttca atttttttaa tagcatcgga gacattgtat tcactattat agatcaaggt
     8641 ataggctcgt ttcaaagcca aaatagtttc aggaggaaaa ttccgtcgct ttaatccaac
     8701 tagattcaaa ccttcataac ggagtggctc acgcgccgcc ataatatagg gcgggacatc
     8761 cttaggaacg cggtaatgtc caccgataat tgaatgctga ccgatccggc taaattgctg
     8821 aacacc
//
LOCUS       DAOMJU010000089         8760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_8400_length_8760_cov_17.506950, whole genome shotgun sequence.
ACCESSION   DAOMJU010000089 DAOMJU010000000
VERSION     DAOMJU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8400_length_8760_cov_17.506950"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..369
                     /locus_tag="PLO58_08085"
     CDS             94..369
                     /locus_tag="PLO58_08085"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HQO74787.1"
                     /translation="MSDIEHRNPAWAGRHPQTNGFVERFNRTVLNEFFRPAFRTKMYE
                     SVEALQKDLDIWLREYNEQRPQRGYRNIGKRPIDRTNEYLKIVSPEG"
     gene            complement(396..1649)
                     /locus_tag="PLO58_08090"
     CDS             complement(396..1649)
                     /locus_tag="PLO58_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HQO74788.1"
                     /translation="MKLEKYSLGIGDRFGHQAEAQLTALVKAAEKGVNITPVWNKSFR
                     EHNIIGSQPTDVRHSAEKAVQALNWSKGYFVDADHINLKTVPHFIESSDFFTLDVADA
                     IGVPPNENDIREFLKFAEKYTRPFGIPGIPQKIAVDHKSIEKIARKYLAAVLAAADTY
                     HYIQDNCQHDYVIELSVDETDKPQSPLELFFILSMAAWQELPLATIAPKFSGRFNKGV
                     DYVGDPAKFAVEFEQDVAVVKFAIKEFSLPDGLKLSVHSGSDKFSLYPHINSIITKTN
                     AGLHLKTAGTTWLEELIGLAAAGGEGLEIAREIYCRAYERYEELVKPYATVVDIDRTK
                     LPAPSVVNGWTSREYVSALRHNQSEKAYNPHLRQLLHVGYKIAAEMGERYLSALKKYA
                     EFIAPNVTENLYQRHIQPLFGVPVD"
     gene            complement(1751..3004)
                     /locus_tag="PLO58_08095"
     CDS             complement(1751..3004)
                     /locus_tag="PLO58_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 domain-containing protein"
                     /protein_id="HQO74789.1"
                     /translation="MSKPRLIIVAMLFLTSVFSCNNRSAKERPDLPLSFKIIVKNPTK
                     QVRKDAMVRINLADIKAKYPDFNPDAWVIFAGETEIPSQLNAEKTEILFVTDFAPSEK
                     KTFTIHYAKEGTITHNYPKRTQAELSHKVGGRFVNRVYEGGEFQNVKYLRVPPEHTDH
                     SWFIRYEGPGWESDKVGYRFYLDWRNAIDVFGKKVPDMVLQEIGQDGFESYHSMCPWG
                     MDVLKVGESLGLGTLGMWYEGKAERIAVTDSIICEIIQDGVLESRIRTRYYGWQIGGQ
                     KYDLVSELSIAAGSRLTRHDIQITGEPPNLCTGIVKMENVSLLSSTDQNRDWMYFATY
                     GKQSLANDKLGLAVLFRRQDLIQITDDENSYVVILKPTAGKLTYYFLAAWEQEPDGIK
                     TESQFADYLNETVAMLDNDLIITIK"
     gene            complement(3052..4314)
                     /locus_tag="PLO58_08100"
     CDS             complement(3052..4314)
                     /locus_tag="PLO58_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HQO74790.1"
                     /translation="MRKIVVMTFLLICLAQIGTASEEFDNLMIVDQSGAGQFTSITAA
                     LAALPDMAYQRVVIFIRNGIYEEKIRLDQNYVTLRGENRDSTIIRFCQKRTDWEASKD
                     YIGPAVVNIYADDIILENLTVENTQPEIEPHAFAIYGKGTRTILRNCNLLGKGGDTVS
                     LWNYQQGLYYHSNCYFRGAVDMVCPRGWCFIRDSQFFEVKTSATIWHDGHYDPDQKFV
                     IVNSTFDGVPGFQLGRHHYDAQFFLINCDFSENMADQPIYLKTYDDVSRNNPYLWGER
                     KYFFNCQKAGKAFDWYRNNLATAPGKPQPEEITPAWTFADRWDPESSAPVTVTAYRID
                     GRTVELTFSESVTVGGNPIFMSQTGFKFSIMPQRFTDNCRLIFFAERPISQKDLTGEM
                     RLVEGKIIASQAYVTERGVARNFRIINN"
     gene            complement(4384..5793)
                     /locus_tag="PLO58_08105"
     CDS             complement(4384..5793)
                     /locus_tag="PLO58_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HQO74791.1"
                     /translation="MANCNEINRQSTDQSSLIKQIRLSILSLNHTDFYLILFLGLFIV
                     CQSTAFAQQLAFPGAEGFSRFTSGGRSGKVIAVTNLNDKGPGSLRSAIETNGARTIVF
                     RVSGTIALKSELTIQNGNLTIAGQTAPGDGICIRNYPLIIGADNVIIRYLRVRLGDEC
                     KVEGDAISAIFQKDIIIDHCSFSWGTDEVASIRDNVNSTVQWCLISESLTHSIHHKGD
                     HGYGGIWGGQGATFHHNLIAHNSSRNPRFNGSRYSHKPEAEYVDFRNNVIFNWGFNSA
                     YGGEGGHQNIIANYYKAGPASECPDRIVEPWDSTGQWYVAENFVFGFPAITADNWAGG
                     VQGKFRNFGRVDQPFAAAPVVTHSAEAAYELVLANCGAVLPKRDAVDERLVAEVRDGI
                     VHFGKNGIIDSQKEVGGWPTLNSTTPVKDADQDGMADDWELANGFNPNDPNDRNGDFD
                     GDGYTNLEEYLNSLDKLYY"
     gene            complement(5777..7186)
                     /locus_tag="PLO58_08110"
     CDS             complement(5777..7186)
                     /locus_tag="PLO58_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HQO74792.1"
                     /translation="MKKLSPVHRHLRLNLTIIILLGMICTWGIAQTPESGWNQVPAIL
                     EKIVPPEFNARDFNITDYGAIGDGQFDCTQAFAKAIDACHKAGGGRVVVPSGKFLTGA
                     IHLKSNVNLHITGEGNILFSRDIHQYLPVVFTRFEGVECLNYSPFIYAYEQENLAVTG
                     SGTIDGQADNETWWNWVMNKKFGWKEGMPNQEKDRDLLMEMADKNVPVKERIFGEGHY
                     LRPNFIQFYKCKNILIEGVTLKQSPMWEIHPVLCENVTVKKVTVISHGPNNDGCDPES
                     CRNVLIKDCYFDTGDDCIAIKSGRNGDGRRLKAPSENIIIQDCRMKDGHGGVVIGSEM
                     SGNVRNVFAEDCIMDSPNLDRALRIKTNSLRGGIVENIYMRNVTVGVVSDAVIRIYFY
                     YAEGDVGQHTPIVRNIYVENVTSQASDYALFLQGYERSPISNVQLSNCRFNGVKKPNI
                     IKNVEGLKMKDVYINGELQ"
     gene            complement(7231..8454)
                     /locus_tag="PLO58_08115"
     CDS             complement(7231..8454)
                     /locus_tag="PLO58_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HQO74793.1"
                     /translation="MKVKNNLKKKHFISGLILITLIPLIGRAALNQEIKPTSAWSVRM
                     AESVIQRHPQPWLLESPSEPKWEYTYGLVMKSILDVWEKTGEQKYFQYVESYYEYFID
                     DSGRILTYRPEEYNLDRINAGKPLFRLYAETGKVKYQKALMNLRQQMATHPRTKEGGF
                     WHKKIYPHQMWLDGLYMAGPFLAQYAVVFNEPALFDDVIKQFILIEEKTRDPQTGLLY
                     HGWDESRQQAWANPETGRSPNFWGRAMGWYAMALVDVLDFLPADHPGRPEIVAILKRL
                     ADAVVKVQDPATGVWYQVLDKGGQPGNYLESSASCMFVYSLAKGVRLGYLDPVYLDAV
                     KKGYSGILNQFIEVDSAGLVNINQACAVAGLGGKPYRDGSYDYYVSAKTARNDPKAVG
                     PFILASLEMEMLNEK"
     gene            complement(8542..>8760)
                     /locus_tag="PLO58_08120"
     CDS             complement(8542..>8760)
                     /locus_tag="PLO58_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO74794.1"
                     /translation="TTQIAFDLDKSGYTTLTIYNLLGQMVDKPVAKHLPAGSYRITFD
                     ASHLAGGMYFYKLECAGQVNVRKMMLLK"
BASE COUNT         2437 a         2112 c         1778 g         2433 t
ORIGIN      
        1 tgccccagta taaacactgg aattttgtcg ttacgacgat tctgaccgat aatagctagg
       61 acttccaacc atccctatga actatgtctg gaattgtcgg atattgaaca tcgtaatccc
      121 gcctgggcgg gacgccatcc gcaaaccaat ggttttgtag aacgattcaa tcgtaccgtc
      181 ctaaatgaat ttttccgtcc ggctttccga actaaaatgt acgaatcagt cgaagcttta
      241 caaaaagatc tggatatatg gctgagagaa tacaatgaac aaagacccca acggggttac
      301 cgaaacattg gcaaacgccc aattgatcgc accaatgaat atttgaaaat tgtttcgcca
      361 gaaggttaat catacccatt ttttgccctc acaatttaat ccacaggcac cccaaacaac
      421 ggctgaatat gtctttggta gagattttcg gtcacgttcg gggcaataaa ttcagcgtat
      481 tttttgagag ccgaaaggta gcgctcgccc atctcagccg caatcttata accgacgtgt
      541 aatagctggc gcagatgcgg gttataggct ttttcactct gattatgacg taaagctgat
      601 acatactcgc gagaagtcca accgttgacg accgaaggcg ccggcaattt agtccgatca
      661 atgtccacaa cagttgcata gggcttgact aattcctcat aacgttcata agcacgacaa
      721 tagatttcac gcgctatttc caagccttcg ccgccagccg cggctaaacc aatcagttcc
      781 tccagccagg ttgtcccggc agtcttgaga tgcagtccgg cattagtttt tgtaataatg
      841 ctattaatat gtggatagag tgagaactta tcactgccgg agtgaacgct aagtttcagc
      901 ccgtccggca aagaaaattc cttgatcgca aattttacca cggcgacatc ctgctcgaat
      961 tcaaccgcaa attttgccgg gtcgccgacg tagtcaacgc ccttattgaa acgtcccgaa
     1021 aatttcggcg caattgtagc cagcggcagt tcctgccaag ccgccatact cagaatgaaa
     1081 aataattcca acgggctttg tggcttgtca gtttcatcaa cagaaagttc aatcacataa
     1141 tcgtgttgac aattatcctg aatgtagtga taagtatcag ccgcggctaa tactgccgct
     1201 aagtatttgc gggcgatctt ttcgatagat ttatgatcaa ctgcgatttt ctgcggaatg
     1261 cctggaatac caaaaggtcg cgtgtatttt tcagcaaatt ttaaaaattc ccgaatatcg
     1321 ttttcattgg gcggaacacc gatggcatcc gccacatcca gcgtaaaaaa atcagaggat
     1381 tcgataaaat ggggcacggt tttgagattg atatgatcgg catcaacaaa ataccccttc
     1441 gaccagttca aagcctggac cgctttctcg gccgaatgac gcacatctgt cggttgactg
     1501 ccgattatat tatgttcgcg aaaagactta ttccagactg gtgtgatatt gacgcccttt
     1561 tcggcggctt tgactagcgc ggttaattgg gcttcagctt gatgcccgaa acgatcccct
     1621 atgcccagtg aatatttttc caatttcatt ttgactattt ccttttagca attaaaaaaa
     1681 ccggtctgta tttacgaatt cttacgaatt taaaaaatga aaattccaga aattaatgtt
     1741 ttccgatgtt ctattttatc gtaataatta aatcattatc cagcattgcc accgtttcat
     1801 tcagataatc tgcgaactgg ctttcggttt taatgccgtc cggctcctgt tcccaggcgg
     1861 ctaagaaata atacgtcaat tttcccgccg tcggtttcaa aatcacaaca tagctatttt
     1921 catcatcggt tatctggatc aaatcttggc gacggaaaag caccgccagt cctaatttat
     1981 cattcgccag actctgttta ccataggtgg caaagtacat ccagtcacga ttttgatcag
     2041 tcgaactaag tagtgacacg ttttccattt tcacaatgcc ggtacacaga ttgggcggct
     2101 cgccagtaat ttggatatcg tgacgggtca agcgactacc ggcggcaatc gagagttcgg
     2161 acaccaaatc atatttttga cctccgattt gccagccata ataacgagtg cggatgcgcg
     2221 attcaagcac gccgtcctgt ataatttcgc aaattatact gtcagtaacg gcaatccgct
     2281 cggctttccc ttcataccac attcccaatg taccgagacc gagcgattca ccgactttca
     2341 gcacatccat tccccaggga cacatactat gatacgattc aaagccatcc tggccgattt
     2401 cttgtaaaac catatcgggt actttcttgc caaagacatc tatggcattg cgccagtcga
     2461 gataaaaacg atagccgact ttatcggact cccaacccgg cccttcatag cgaatgaacc
     2521 acgaatgatc ggtatgttcg ggcggtaccc gcaaatattt tacattctga aattctccgc
     2581 cttcataaac ccggttgacg aaacgtccgc cgactttatg agataattcc gcctgcgttc
     2641 gcttgggata attgtgagta atcgttcctt ccttagcata atgaattgtg aaggttttct
     2701 tctcggaagg agcaaaatcg gtaacgaata aaatttcggt tttctccgca tttagctgag
     2761 aggggatttc cgtctcaccg gcgaaaatta cccaagcgtc cggattaaaa tccgggtatt
     2821 tggcttttat gtcagccaga tttattctaa ccatcgcgtc tttacgaacc tgtttggtag
     2881 gattttttac aattatttta aaggaaagcg gcaggtctgg acgctcttta gcagaacggt
     2941 tattacaaga aaaaacactt gttaaaaaca gcattgctac gattattaat cttggtttag
     3001 acattggtga ctccctgata ttatttttat tttttaacct aaaattcata tctaattgtt
     3061 aataattcta aaattacgag ctacgcctcg ctcggtaacg taagcttgcg aggcaattat
     3121 tttcccttct acaagcctca tttcacccgt caggtctttt tgggatattg gccgctctgc
     3181 aaaaaatatc aagcggcaat tgtcggtaaa acgctgaggc ataatgctaa atttaaagcc
     3241 ggtctgactc ataaaaatcg gattgccacc taccgtcaca ctttcactaa aagttaattc
     3301 aactgttcga ccatcaatgc gataagctgt aaccgtaacc ggcgcgcttg attccggatc
     3361 ccagcggtca gcaaaagtcc aggctggtgt tatttcttcc ggttgcggtt ttccaggagc
     3421 tgtcgcgaga ttattacgat accaatcgaa agctttgccc gctttctggc aattaaagaa
     3481 atatttacgt tcgccccaca aataaggatt attgcgactg acatcgtcat aagttttcag
     3541 atagatcggc tgatccgcca tattttccga aaaatcacaa ttgattagga aaaattgagc
     3601 gtcgtaatga tggcgtccca gctgaaaacc cggcacgccg tcaaaagtag aattgactat
     3661 aacgaatttc tgatccgggt catagtgacc atcatgccag atcgtagccg aagtctttac
     3721 ttcgaaaaat tgcgaatcgc ggataaaaca ccagccgcgc gggcaaacca tatcaaccgc
     3781 cccgcgaaaa taacaattag aatggtaata caaaccctgc tgataattcc agagcgaaac
     3841 ggtatcgccg ccttttccca gcaggttgca gttccgtaaa attgtgcgag ttccttttcc
     3901 ataaatagcg aaggcatgcg gttcaatctc cggctgagta ttttccaccg tcaaattttc
     3961 caaaataatg tcgtcagcat agatattgac gactgccggt ccgatataat ccttgctggc
     4021 ttcccaatca gtgcgtttct gacagaaacg gataatggtg ctgtcgcgat tttcaccgcg
     4081 aagagtgaca taattctggt ctaatcgaat tttttcctcg taaataccat ttcgaataaa
     4141 aataaccacc cgttgataag ccatatccgg taaagccgcc agcgccgccg tgatcgaggt
     4201 aaattgtccc gcgccacttt gatcaacaat catcaggtta tcgaattctt cggaggcagt
     4261 tccgatttga gccagacaaa tcagcagaaa cgtcatgacg acaatttttc tcataaaaga
     4321 cctgcagtta taacgacgct accacttttt tcaattacca gatttacctt tttccgaaac
     4381 attttaataa tataatttat ccagactatt taaatattct tcaagattag tgtaaccgtc
     4441 accgtcaaaa tcgccgttac ggtcgttcgg gtcgttggga ttaaagccat ttgccagttc
     4501 ccagtcgtct gccataccat cctgatctgc gtctttgacc ggtgtagtag aattcaaggt
     4561 cggccagccg ccaacttcct tctgagaatc gataatgcca tttttaccaa agtgaacaat
     4621 cccatcccga acttccgcca ccagacgttc atccaccgca tcgcgtttgg gcagaacagc
     4681 accgcagttt gccaatacta attcgtatgc cgcttccgcc gaatgagtaa cgaccggcgc
     4741 cgcggcaaat ggctgatcaa cgcgcccaaa attgcgaaac ttaccttgta caccgcccgc
     4801 ccaattatcc gccgtaatgg ctggaaatcc aaacacaaaa ttttcggcaa cgtaccactg
     4861 accggtgctg tcccatggct cgacaattcg gtcgggacac tccgaagcag gaccggcttt
     4921 ataataattg gcgataatat tctggtggcc accttcgccg ccgtaggcac tattaaaacc
     4981 ccaattgaaa atcacattat tgcggaaatc aacgtactcg gcttcaggtt tgtgactata
     5041 gcgactgcca ttgaagcgcg gattgcgact ggaattatgc gcaattagat tatgatggaa
     5101 agtcgcgcct tgaccgcccc agatgccgcc ataaccgtgg tcgcctttgt gatgaatcga
     5161 atgagtaagg ctctcgctga ttagacacca ttgaacggtg gagttgacat tatcgcggat
     5221 cgaagcgact tcatccgtgc cccagctgaa cgaacaatga tctataataa tatctttctg
     5281 gaaaatggca gaaatggcat cgccttcaac tttacattcg tcacccaacc tgacacgaag
     5341 atagcggatt atgacattat cagccccaat tatcaggggg taattacgaa tacaaatacc
     5401 gtcgcccggc gctgtttgac cagcgatcgt tagattgcca ttctgaatag tcagttcgga
     5461 ttttaaagca atcgtgcctg aaaccctgaa aacaattgtt cgggcgccat tggtttctat
     5521 tgcggatcgc aaactgcccg gacctttatc atttaagttc gttacggcaa tgactttacc
     5581 actgcggcca ccggaggtga accggctaaa tccttccgcg ccaggaaatg ccaattgctg
     5641 agcaaatgcc gttgattggc agacgataaa caaaccgaga aacaagatca ggtaaaaatc
     5701 ggtatgattc aaagatagta ttgataaacg aatttgtttt attaaggacg attgatcagt
     5761 tgactggcga ttgatttcat tgcagttcgc cattgatata gacgtccttc atctttaagc
     5821 cttccacatt tttaataatg ttgggctttt ttacaccgtt aaaacgacaa ttactaagtt
     5881 gcacattaga tataggcgac ctttcataac cttgtaaaaa taaagcataa tcgcttgcct
     5941 ggctggttac attttcgacg taaatgttac ggacaatcgg cgtatgctga ccaacgtctc
     6001 cttcggcata gtagaagtaa attcgaataa ccgcgtcact gacaaccccg acagtcacgt
     6061 tgcgcatgta gatattctcg acgattccac cccgcaggga attggtttta atccgcaaag
     6121 ctcgatccag gttaggacta tccataatac aatcttccgc aaagacattc cggacgttgc
     6181 ccgacatttc actgccgatt actacaccac catgcccgtc tttcattcga cagtcttgaa
     6241 taatgatatt ttcgctcggg gctttgagac gtcgtccgtc gccattgcgt ccggatttta
     6301 tggcgataca atcatcgcca gtatcgaaat aacagtcttt gatcaagaca ttgcggcaac
     6361 tttcggggtc acagccatcg ttattaggac cgtggctgat gacggtaact tttttgacgg
     6421 ttacgttttc acacagcacc ggatgaattt cccacattgg tgattgctta agcgtaacgc
     6481 cttcaatcag aatattttta cacttataaa actggataaa attcggccgt aaataatggc
     6541 cttcaccgaa gatacgttct tttaccggta catttttatc tgccatttcc atcaataaat
     6601 cacggtcttt ttcctgattc ggcattcctt ctttccagcc gaatttcttg ttcatcaccc
     6661 aattccacca ggtttcatta tcagcctgac catcaatggt acccgatccg gtgacagcca
     6721 gattctcttg ttcataggca tagataaaag gcgaataatt cagacattcc accccttcaa
     6781 atcgggtaaa tacgaccggc aaatattgat gtatgtctcg gctgaataga atatttcctt
     6841 caccggtaat atgcagattg acattacttt tcagatgaat agcacccgtc agaaatttac
     6901 cggacggtac gacgacgcgt ccgcccccgg ctttatgaca ggcatcaatt gccttagcga
     6961 aagcctgagt gcaatcgaat tgaccatcac cgatagcgcc gtaatcagtg atgttgaaat
     7021 ctctggcatt gaattcgggg ggaacgattt tttccagaat tgctggtact tgattccaac
     7081 ccgattcggg agtttgagca attccccagg tacaaatcat tcccagcaga atgataattg
     7141 tcagattcag acgtagatgg cgatgaacag gcgataattt tttcattttg tactagttac
     7201 actttcattt atttatgcaa ataattattt tcacttttca ttcagcattt ccatttccag
     7261 acttgccaga ataaatggcc cgacggcttt gggatcgttg cgcgcagttt tggcgctcac
     7321 ataataatcg taagaaccgt cacggtaagg tttaccgccc agcccagcta ctgcgcaagc
     7381 ttggttgata ttgaccagtc cggcggaatc cacttcgata aactgattca aaatgccgga
     7441 atatcctttc ttaactgcat caaggtaaac cggatcaaga tagccgagcc gaacgccttt
     7501 ggctaacgaa tagacaaaca tacaggacgc cgaggattcc aaataattac ctggctggcc
     7561 acctttatcc agaacttgat accaaacgcc ggttgccgga tcttgaactt taacgacagc
     7621 atccgctaat cgttttaaaa tagcaacgat ttctggtcgc cccggatgat cggcgggtaa
     7681 aaaatcgagc acatcaacca gcgccatcgc gtaccatccc atcgcgcgcc cccaaaaatt
     7741 gggagaacga ccggtttctg gattagccca ggcttgctga cgactctcgt cccagccatg
     7801 gtagagcaaa ccggtttgtg gatcgcgcgt tttttcttcg attaaaataa attgcttgat
     7861 gacatcatca aataatgccg gctcattaaa gaccaccgca tattgagcta aaaaaggtcc
     7921 tgccatatat aaaccatcca gccacatttg atgcggatag attttcttat gccagaaacc
     7981 accttcttta gtgcgcggat gagtcgccat ttgttgccgc aaattcatca gcgccttttg
     8041 gtatttgacc ttgccggttt cggcatagag tcgaaaaagg ggtttaccag cattgatccg
     8101 gtcaaggtta tactcttctg gacggtaagt taaaatgcgg ccgctatcgt caataaaata
     8161 ttcataatag gactcgacat actggaaata tttttgttca ccggtttttt cccaaacatc
     8221 cagaatggat ttcataacca gtccgtaagt atattcccat ttaggttcac tcggactttc
     8281 gagcaaccag ggttgcggat gccgctggat gactgattcc gccatccgca ccgaccaggc
     8341 tgaggttggt ttaatttctt gattcaatgc cgctcttcct attagaggaa taagggtaat
     8401 cagaatcaat cctgatataa aatgtttctt cttaagatta ttttttactt tcattttaac
     8461 tcaattggta aaaatatggg ttgggaggac attacatcct cccaacccat tacccatttc
     8521 agattaatat tctattagaa atcacttcaa caacatcatt ttacgaacat taacctgacc
     8581 agcacattcc agtttataaa aatacatacc accagctaaa tgactggcat cgaaagtaat
     8641 ccggtacgaa ccagccggta agtgtttagc aaccggtttg tcaaccattt gccccaacaa
     8701 gttatagatc gttagagtcg tataaccgga tttatccaga tcaaatgcaa tctgagtagt
//
LOCUS       DAOMJU010000090         8653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_8532_length_8653_cov_22.143173, whole genome shotgun sequence.
ACCESSION   DAOMJU010000090 DAOMJU010000000
VERSION     DAOMJU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8532_length_8653_cov_22.143173"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..1360
                     /locus_tag="PLO58_08125"
     CDS             407..1360
                     /locus_tag="PLO58_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQO74795.1"
                     /translation="MANKTIELKGVDPALVFGVRDVFINKIEESFGARIFARGTKINL
                     EGDENTLSQIERVIGQMLMTINRKGFVDEQDVTTLINMELGGQQLPGEITADTVILFK
                     KTGVIKPRSVNQEHYFSATLKNDLVFGIGPAGTGKTYLAVAIAVAALRNHDVQRLILT
                     RPAVEAGESLGFLPGDLTEKIEPYLAPLYDALYDMLPPEKVRVYEEQHIIEVLPLAYM
                     RGRTLNNAYVILDEAQNTTPMQMKMFLTRLGANSKAIVTGDITQIDLPDRQKSGLIDA
                     LEILRGIEGIEFVYFDTTDVVRHRLVKKIIKAYNGESPNKS"
     gene            complement(1571..2428)
                     /locus_tag="PLO58_08130"
     CDS             complement(1571..2428)
                     /locus_tag="PLO58_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74796.1"
                     /translation="MRKISKITLLEMGVLMALLPSCKKATVQSAWNDGSIIIDAVVKD
                     WGSRLVWNEKSQVSLGVTNDAENLYLCLVTSDPDIHRQILINGFIVGIDQKGSKSHHF
                     GIKFPLGIGDRGNQLLMPTDDQRRQKPFEPSQPGQKGRENDFLAKFNEQQTELILLGP
                     GDKQKTLVPLENDLGLEVKIGFDRQQLVYELKLPFSVISQHLQIDQISNNKPLTVSFK
                     TDKVEIPSGRPGDMMDSPGGMGRPEGGMGGPGGRMGVPDARMGNPGGDEHSEKTFSYQ
                     VAVTLAVKP"
     assembly_gap    2880..2979
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2980..3239)
                     /locus_tag="PLO58_08135"
     CDS             complement(<2980..3239)
                     /locus_tag="PLO58_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74797.1"
                     /translation="MKKTTRTTLMLSSVLILGIIIGFVGASSWLHYNQKKRFTHFRKG
                     EGFVVEMEKMIDPLPEQKDKIHEILVRHSLWVKQFSEEQRAI"
     gene            complement(3245..3673)
                     /locus_tag="PLO58_08140"
     CDS             complement(3245..3673)
                     /locus_tag="PLO58_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74798.1"
                     /translation="MKIDKIRKLLLLSGDRNLTAKEQKILMSARQTNPELAEDEQLLQ
                     KIQQNLAGTKQESFEPWFAERTMNRIRQLASQEKHPARLWNGFWPAYSRVIIGAAVIL
                     ALIVVFNLFTSSEPSLAKAFGITQTPIEAIMDPIAYLIWE"
     gene            complement(3670..4233)
                     /locus_tag="PLO58_08145"
     CDS             complement(3670..4233)
                     /locus_tag="PLO58_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQO74799.1"
                     /translation="MENLASLTDQEIIDACLSGNENAFAEIIRRYEPRVAATVIGMLG
                     RCPEAEDVGQETFIRFYKSMSKFRGESSLSTYLTRIAMNLSINELNRRKRQSRFMPLD
                     DPEISRKTSGDPGNQADSESGAIIRRAVAQLAPKFRSTIVLRLIEGYSTEETAQILQL
                     PVGTVLSRLARAKVQLMNLLTPYKELL"
     gene            4383..5651
                     /locus_tag="PLO58_08150"
     CDS             4383..5651
                     /locus_tag="PLO58_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQO74800.1"
                     /translation="MKKKICLIVILVLAVAVGWHLIRKNGSDKQIQIAYGQVTRGNIE
                     TIISSTGTLVAIGTVDVGTQVSGRIDKVLVDYNDIVKKDQVLAVLDTLLLSQSVKEAE
                     ANLHKMQALYEQAAFQYDRISNLFQQKLVSEQEYIDARTNKATSEVSLKQAQIALDKA
                     VADYGYAIIRSPISGTVIDRVVEEGQTVAASFSTPTLFTIARDMTKMEIEALVDESDI
                     GEIKTGQKVRFTVQSYQDKIFNGVVRQVRLQPQTVSNVVNYTVIVDADNHEQLLLPGM
                     TATVDFIIDSRENVLIVPNSALKITPTEEMLAKAHQVRQAQMEQLPESVKTKLREQSP
                     KPQLADLSPEELDKSTRPTPIWYVNESGQILMALVKTGLSDTKNTEILENPEIKEGTK
                     IIVSLIANGKNSVSNSSNNQQRGMGGPPPF"
     gene            5698..6453
                     /locus_tag="PLO58_08155"
     CDS             5698..6453
                     /locus_tag="PLO58_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQO74801.1"
                     /translation="MPIVIEVRQLSKTYQVGDIEVHALRKVDFAVAAGEFTAIMGASG
                     SGKSTLMNMLGCLDQPTGGEYLLDGVNVSTLTSDERADIRNQKIGFVFQGFNLLPRTT
                     ALENVELPLIYDRHQRIKNPQSAAKEALERVGLGDRMHHIPTQLSGGQQQRVAIARAL
                     VNQPSILLADEPTGNLDSKTSMEIFSLFQQLNDEGLTIVMVTHERDFATYAKRILQMR
                     DGVLHKDTLIKKRRNAAEDLKMLIAKQQQEEEQ"
     gene            6450..>7069
                     /locus_tag="PLO58_08160"
     CDS             6450..>7069
                     /locus_tag="PLO58_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQO74802.1"
                     /translation="MKKIYNLLKVAIKSIFKNRMRSLLTSLGIIIGVSAVIIMVAVGR
                     GSQAAIEDNIKSLGTNLIIIFPGASRSGGASMGAGSFNRFTMKDVERIKESATLISAI
                     SPVVRANGQVIYKSNNWYTSISGVSPDYFSITNWELESGEFFTERDDLARKKVALLGK
                     TVATELFGADNPVGEQIRIRNVPFTVIGVLKEKGGSGPGGDQDDII"
     assembly_gap    7070..7169
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7170..7336
                     /locus_tag="PLO58_08165"
     CDS             <7170..7336
                     /locus_tag="PLO58_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQO74803.1"
                     /translation="ASALLNNLTDLRTVIDPVIVILAFTFSGAVGIFFGWYPARKAAN
                     LNPIDALRYE"
     gene            7352..8572
                     /locus_tag="PLO58_08170"
     CDS             7352..8572
                     /locus_tag="PLO58_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQO74804.1"
                     /translation="MKRIKIIGLFMLMATTICAADELTLEQCIEAAVANNKSLVKNEL
                     GVQQTAIGTKTAYGTLLPSVSASASQSGGDGSSRSSASLQINQKIYSPGLFSDIKLAR
                     NETDIARLNYNELASQIRSEVETAYYNILTAQSLLNVYQENLTAADENLQLIQTMYKL
                     GTKTESDVLKAEVQKAEIENELIGQELEIINEKRSLAILMGISPDYDFEIVEIDVEQI
                     EIPPLAEAKELLFKNNPEYQSLLKSLKSEQISLQTARKSYLPSLSADYSYSKDWTGST
                     TTEGYGSWGLSLNLGLFNGFSKKLNVQKSKLSLRSAEVSLEAKEQELLATLLNYYSNF
                     ENHNKVIALQEKNLESARKDYELVTRQCELGLATMLEQTNAQVSLLSAQTNLVEAQYS
                     RKIVESKIKQLLAL"
BASE COUNT         2440 a         1831 c         1860 g         2322 t
ORIGIN      
        1 gaaaagaggt tgagaaaaga gaatggtcat tagtcattgg tcattagtca ttagtcatta
       61 gtaaggagac tgagtaaaga ggttgagaaa agagactgag gctgagatta gtcattggtc
      121 attggtcatt agtcattagt aaaaaggcta agactgaaga agaacttaaa tcttagcctt
      181 ttttctcagc cttaaccttt cttaagctga ggctgagtaa agagattgag taaatagatt
      241 gaggttgaga aagaattcaa atcttagcct tctttctcag tcttaacctt tctttaacga
      301 gtttacaact cgactgacgg gatcatccgg ttggtttctc gcatggcttt cattttgcca
      361 ataaaattgc gaaattagca attaatgaaa gcattgatgg agaaatatgg caaataaaac
      421 catcgaatta aaaggagtcg atccagctct ggtgttcggc gtccgagacg tatttatcaa
      481 caaaatcgag gagagcttcg gggcgcgaat attcgcgcgg ggcacgaaaa taaatctcga
      541 aggcgacgaa aatacactta gccaaattga gcgcgtgatc ggtcaaatgc taatgacaat
      601 caatcgcaaa ggatttgttg acgaacagga cgttaccact ttaatcaata tggaacttgg
      661 cggacagcaa ttgccgggcg aaattactgc cgataccgtc atcctcttca agaaaaccgg
      721 cgtaatcaaa ccgcgctcgg taaatcagga acattatttc agcgccacct tgaaaaacga
      781 cttagtgttt ggcattggtc cagctggaac tggcaagact tatctggcgg tcgccatcgc
      841 tgtggcggct ttgcgcaatc acgatgttca acgcttaatc ctgacgcgcc cagctgtcga
      901 agctggtgaa agtctcggat ttctgccggg cgacctaacc gaaaaaattg aaccttatct
      961 tgcgccgcta tacgatgcac tttatgatat gttgccgccg gaaaaagtca gagtttacga
     1021 agagcaacac ataattgaag ttcttccgct ggcttatatg cgggggcgca cgctaaacaa
     1081 tgcctacgta attctggatg aagcccaaaa caccacgcca atgcagatga aaatgttcct
     1141 aacccgcttg ggagccaatt ccaaagcgat tgtcaccggc gatataaccc agattgattt
     1201 gcccgatcgc cagaaatctg ggctaattga tgcgctggaa atattgaggg gcattgaggg
     1261 aatcgaattt gtttacttcg ataccaccga tgtcgtacgc caccgcctgg ttaaaaagat
     1321 tataaaagct tataatggcg aaagtccaaa taaatcctga ctgtagtcat ttcctaaaat
     1381 ccggtagtta attgcctttc ttgatgataa gacaataata attgttcagt taaaaaaggt
     1441 gttatataat cagcaggcaa cggaagactc atacgagtct tccgtgtatc ctgctgaacc
     1501 gccgtggagg agtgtaggcg agtgagtgat agagatatga gggttattat tcaaaagggt
     1561 ttaccaaatc ttatggtttc actgccaaag taacagccac ttgatatgaa aatgtctttt
     1621 cgctatgttc atcaccgccg ggattgccca ttctagcgtc gggaacaccc atcctaccgc
     1681 caggaccacc cataccacct tccggtctgc ccattccgcc gggggagtcc atcatatcac
     1741 ctgggcgacc ggatggtatc tcaactttat cagttttgaa actgactgtc aagggcttgt
     1801 tatttgaaat ttgatcaatt tgcagatgct gactaatcac gctaaatggc aatttcagct
     1861 catagacaag ttgctggcga tcgaatccga ttttgacttc cagtcccaag tcgttttcca
     1921 gaggaacaag cgttttttgt ttatcacctg gtcccagcaa aattaactcg gtttgctgtt
     1981 cattaaattt agccaaaaaa tcattttccc tacctttctg ccccggttga ctgggttcaa
     2041 aaggcttctg tcggcgttgg tcatcggtcg gcattagtag ttgattgccg cggtcgccta
     2101 tacccaatgg aaatttgata ccgaaatgat ggcttttact acccttctga tcaattccga
     2161 caataaaacc gttgatcaat atctgacggt ggatgtccgg gtcggaagtt accaaacata
     2221 agtataaatt ttcggcatca ttggtgactc ccaaactgac ctggcttttt tcgttccaca
     2281 ccaagcgtga gccccagtcc ttaacaacgg catctattat tatactgcca tcgttccagg
     2341 cgctctgaac tgtagccttt ttacaactag gcagaagtgc cattaaaacg cccatttcta
     2401 ataatgttat ttttgatatt tttcgcattt ttccctttcc acaaaattgt tttgaaattt
     2461 atttaccgta caaccgtttt atcctaataa cgacttgctt aaaatcgttt actttcaagt
     2521 gatttatttc tcgttacaaa aaactcaaac tcgcagttta gacaaagcca atcctatttt
     2581 tattccattt taaaatgaaa cgaaagttaa ttatgctccg gctttttgtt aggtctgctt
     2641 aagaaatcaa aatgtaggca agttccgata atggacaagt tagttctttt tagatggatt
     2701 caaactctgt ggggttggtg atattgtttg cctatagtta ttttaactcc cgcggagctg
     2761 taggaggtga ggctttataa tcatataatt catgtatatt caatcccgac aggtcggtgt
     2821 tggatataaa tgtaaatcat agtatcgtgt ttattttttc aggaaacccc acttaacatn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aaatagcgcg ttgctcttcg
     3001 ctaaactgtt tgacccagag tgaatggcgt accaatattt cgtgaatttt atctttttgt
     3061 tccgggagtg ggtcaatcat tttttccatt tcgactacga agccctcgcc tttgcggaaa
     3121 tgagtaaaac gttttttctg attatagtgt agccaacttg aagctcccac aaaacctata
     3181 ataattccca aaatcaggac gctacttagc attaaagttg ttctggttgt tttcttcatt
     3241 tttattactc ccaaattaaa tatgctatcg gatccattat ggcttcgatt ggagtctgag
     3301 taataccgaa tgcctttgcc aggctcggct cggaactagt gaagagatta aaaaccacaa
     3361 tcaaagccag aattactgcg gctccaataa taacccggct ataagccggc cagaaaccat
     3421 tccacaatct tgctggatgc ttttcctgac tggcgagttg gcgaattcgg ttcatcgtcc
     3481 tttcagcaaa ccagggctca aaagactcct gttttgtgcc agcaagattt tgttgaattt
     3541 tctgtaatag ttgttcatct tccgccagtt cgggattagt ttgtctagct gacataagaa
     3601 tcttttgttc cttggcagtc aggttacgat cgccggatag aagtaatagc ttgcgaattt
     3661 tatcaatttt cataatagct ccttataggg tgttaataaa ttcataagtt ggactttggc
     3721 gcgtgctaaa cgcgacagca ccgtcccaac gggcaattgc aaaatctggg cagtttcttc
     3781 agtcgaataa ccttcgatca gacgcaaaac aatggtcgaa cgaaatttcg gcgccagttg
     3841 agcaaccgcc cgacgaataa ttgcgccgct ttctgaatca gcctgattcc cgggatcgcc
     3901 tgatgttttc ctcgagattt caggatcatc cagaggcata aaacgtgact ggcgcttgcg
     3961 gcggttcagt tcattaattg ataaattcat cgctatccgg gtcagatatg tgctcaaact
     4021 cgattctcct cgaaatttgg acatactttt ataaaaacgg ataaatgttt cctgcccaac
     4081 gtcctcggct tccggacatc ttcccagcat tccaataacg gtagccgcca ctcgcggttc
     4141 ataacggcga atgatttcgg caaatgcgtt ctcatttcct gaaagacagg catcaattat
     4201 ctcctgatcg gttagtgacg ctaaattctc cattatttct caaaacatta agttagacca
     4261 ataggctgtt gaatttattc caatccattc agatataaaa aatagtttgt tcgatttgga
     4321 atgaaatcac tcattatttt gtctaatcag cggttttgaa aattgaaagg atttaaagat
     4381 ttatgaagaa aaaaatttgc ttgatcgtca ttctggtttt ggcggtcgcc gtcggatggc
     4441 atctaattcg gaagaacggc tcggataaac agatacagat tgcctatggg caagtaaccc
     4501 gcggcaatat cgaaaccatc atctccagta ccggtacgct ggtagcgatc ggtacggtgg
     4561 acgtcggtac ccaggtttcg ggccggattg ataaagttct ggtggattac aatgacattg
     4621 ttaagaaaga tcaagttctg gcggttttgg atacactgct attgagtcaa tccgttaaag
     4681 aggcagaagc caatttgcac aaaatgcaag cgctttacga acaagccgct tttcagtatg
     4741 atcgcatttc taatcttttc cagcaaaaat tggtttcgga gcaagaatac attgatgccc
     4801 gaaccaataa agccaccagc gaagtcagct taaaacaagc ccagattgcc ctcgataagg
     4861 cggtcgccga ttatggttat gccatcatcc gctcaccaat ttccggaacg gttattgacc
     4921 gagtggttga agagggacag acggtcgcgg ctagtttttc gacgcctact cttttcacta
     4981 ttgcccgaga tatgaccaaa atggaaattg aagccctagt ggacgaaagt gatatcggtg
     5041 aaatcaaaac cggccagaaa gtgcgcttta ccgtccagag ttatcaggat aaaattttca
     5101 atggagtcgt aagacaggtt cgtcttcagc cgcaaaccgt ctccaatgtc gtcaattata
     5161 cggtcattgt tgatgccgat aatcacgaac aactgttatt accgggaatg accgctaccg
     5221 tggattttat cattgattca cgcgaaaacg tgctgatcgt gccaaattca gcgttaaaaa
     5281 ttactccgac ggaagaaatg ctggcgaaag cacatcaggt gcgacaagca caaatggaac
     5341 aattgcctga gtcagtaaaa accaaactcc gagaacaatc tcctaaacca caattggctg
     5401 atctatcgcc ggaagaatta gataaatcaa cgcgaccgac gccaatctgg tatgtcaacg
     5461 aatccggcca gattcttatg gctttggtga aaaccggttt gagcgatacc aagaataccg
     5521 agattttgga aaatcctgaa attaaagaag gaactaaaat tattgttagt ttaattgcca
     5581 acggcaaaaa ttcggtcagc aattcttcca ataatcagca acgcggtatg ggcggtccgc
     5641 ctccatttta atcacaaaaa tcaaccgata aaatacatac agaattgaga gaataatatg
     5701 ccaatcgtaa ttgaagtccg gcaattgagt aaaacctacc aggtcggtga tatagaagtg
     5761 catgccttgc gcaaggtaga tttcgcagtg gcggcgggtg aatttactgc cataatgggt
     5821 gcttccggct ccggaaaatc aaccctaatg aatatgctgg gttgtttgga tcaacctacc
     5881 ggcggggaat acctattgga tggtgtcaac gttagtacgc tgacgtctga tgagcgcgcc
     5941 gatatccgca accaaaaaat tggattcgtc ttccagggat tcaatcttct accacgcacc
     6001 accgctctgg aaaatgtcga actgcccttg atttatgatc gccatcaacg tataaagaac
     6061 cctcaaagcg ccgccaaaga agcgctggaa cgcgttggcc ttggggaccg tatgcaccat
     6121 attcctaccc agctttccgg cggtcagcaa cagcgggtcg ccattgctcg agcgctggta
     6181 aaccagcctt cgattctact ggctgacgaa cctaccggca atctggacag caaaacgtct
     6241 atggagattt ttagtctctt ccagcaatta aatgatgaag gtttaacaat tgttatggtt
     6301 actcacgaac gcgattttgc cacttatgcc aaacgtattc ttcaaatgcg tgatggagtt
     6361 ctccataagg ataccctaat taaaaaacgg cgtaatgcgg cggaagacct taaaatgcta
     6421 atcgccaaac agcaacaaga ggaggagcaa tgaaaaaaat ttataacctc ttgaaagttg
     6481 ctatcaaaag catatttaaa aaccggatgc gcagtctgct aacctcgctc ggaataatca
     6541 tcggcgtcag cgccgtcatc attatggtgg cggtcggccg cggttcgcag gccgctatcg
     6601 aggataatat aaagtcgctc ggcaccaatc tgataatcat cttccccgga gcctcgcgta
     6661 gcggcggtgc cagtatgggc gctgggagtt ttaaccgttt tacgatgaaa gatgtagaaa
     6721 gaattaagga atcagctaca ttaatcagcg ctatttcgcc ggtcgtccgt gctaacggtc
     6781 aggtgattta taaaagcaat aattggtaca cgagtatcag tggcgtttca ccggactatt
     6841 tttcgataac caactgggaa ctggaaagtg gagagttttt tacggaacgc gatgatttag
     6901 cccgcaaaaa agttgccctg ttagggaaaa cggtggctac ggagctattt ggcgcggata
     6961 atccggtggg tgagcaaatc cgcattcgca atgttccttt taccgttatc ggcgttctta
     7021 aggaaaaagg cggctcggga ccgggaggtg accaagatga cattattttn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggcttcagcc cttttaaata atttgaccga
     7201 tttgcgaacc gtgattgatc cggtaatcgt aatactggca ttcacttttt cgggtgcagt
     7261 tggtattttc ttcggctggt atccggcgcg caaagccgct aatctcaatc cgattgatgc
     7321 cctgcgttat gaatagaaag gatttgttct tatgaaaaga attaaaataa ttggcttgtt
     7381 tatgttaatg gctacgacca tttgcgccgc agatgagctt actctcgaac aatgtatcga
     7441 agcggcagtt gctaataaca aatcgctagt aaagaatgaa ctcggcgttc agcaaactgc
     7501 aattggcacc aaaaccgcct atggaactct actgccatcg gtgagtgcca gcgctagcca
     7561 aagcggcgga gatggcagtt cgcgatcgag cgctagtttg caaattaacc agaaaatcta
     7621 ttcgcctgga ttattttccg atataaaatt agcccgcaat gagactgata tagctcgtct
     7681 taattacaac gagttagctt cccagattcg gagtgaagtc gaaaccgcct attataacat
     7741 tttaactgcg caaagtttgt tgaatgtcta tcaggaaaac ctgacggcgg ctgatgaaaa
     7801 tctgcaatta attcagacga tgtataaact cggcacgaag accgaatctg atgtattgaa
     7861 agccgaagtg cagaaagcgg aaatcgaaaa tgaattgatt ggccaggaac tcgaaattat
     7921 taatgaaaaa cgctcactgg cgattctgat gggaatatct cccgactatg attttgagat
     7981 tgtcgagatt gacgttgaac aaattgaaat cccgccttta gccgaggcta aggaactatt
     8041 gtttaaaaat aacccagaat atcagtcttt gttaaaatcg ctaaaaagcg agcaaatcag
     8101 cctgcaaact gcccggaagt catatctgcc ctcgcttagc gctgattata gctattcgaa
     8161 agattggact ggttctacca cgaccgaagg ctacggttcg tggggtttgt cattaaatct
     8221 cgggcttttc aacggattca gtaaaaagct aaatgtccag aaaagcaaac tcagccttag
     8281 aagcgccgaa gtcagtttag aggctaagga acaggaattg cttgccactt tgctgaatta
     8341 ctattccaat tttgaaaatc ataataaagt tattgctctg caggaaaaga atctggaatc
     8401 agcccgcaaa gattacgaac ttgttacgcg ccaatgcgag ctcggtctgg caactatgtt
     8461 ggaacagaca aacgctcagg tctcgcttct ttcagcccaa accaatcttg tcgaagccca
     8521 gtattctcgt aaaatcgtcg aatccaaaat taaacaactg ctggcattat gatagcgcga
     8581 tgaggcaaat cattcgttgc tataagccat cattcatcgt tgagttcacc cttcgatgag
     8641 ctcagggcga cat
//
LOCUS       DAOMJU010000091         8078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_9396_length_8078_cov_22.684283, whole genome shotgun sequence.
ACCESSION   DAOMJU010000091 DAOMJU010000000
VERSION     DAOMJU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9396_length_8078_cov_22.684283"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1525
                     /locus_tag="PLO58_08175"
     CDS             320..1525
                     /locus_tag="PLO58_08175"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HQO74805.1"
                     /translation="MKVLTLNSGSSSVKYQFIDTSTRTVLCKGLVDRIGISGTSLTHK
                     RQDGHQVVITDNIVDHQTAINSIMDILIDPTHGVAQSLKEIEAVGHRLVHAGASFKQS
                     VLINEAVREKLYECIEFAPLHNPHNIKGIEASLKKLPHTPNVGVFDTTFHINMPDYAY
                     LYAIPYSYNEKYGMRRYGFHGTSHYYVANRAAEIIGKPIEQLNIITCHLGNGCSIAAV
                     RGGISVDTSMGFTPLEGLVMGTRCGDIDPGLILTIIDRENFSIEEANTFFNKQCGMKG
                     LSGLSSDMREIENAAAAGHQRARQALDIFCYRVKKYISAYIGVLNGADLIVFTGGIGE
                     NSALVREKSLSNLENLGILLDPAHNKIAHGVEGIISSAESKINVLAVPTNEELVIALE
                     TEKVIITSQ"
     assembly_gap    1675..1774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1850..5212
                     /locus_tag="PLO58_08180"
     CDS             1850..5212
                     /locus_tag="PLO58_08180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQO74806.1"
                     /translation="MSENKLTRKDWNLLVICGALLLISIIYLSRNYRKAFPAYDLQFD
                     ITRADSRDLAEKFLSHQHIDLNDYRYAVVFDYDELPKTFIEKEVGFEEATRLLCQDFP
                     IWHWSNRWFKPLSPEEIKVNLALDGRVTYFEHFLPEEAAAERISVEAARRICQDFLMT
                     TMQMNPAEWEFIEDKTDIKPNRVDHFFTYQKKGVEISSATYRLEIGIVGGKVGKYREY
                     WKLPEQWLSNYKHLRSLNSTTALAADVLFVLILIAALVVFIIQLSRQNVHLKTALGFG
                     LITFILQFAGNINELPITIYAFDTNQSLGNYYGNYFIQTFVIALLYALLVAVITGAGE
                     VLYRRAYPQHLSMSKLFSPTGLRTKSFLINSTLGMILAVVFIAFQTAFYLIANRFGAW
                     APVEVNYSNAINTYFPWIFVLLIGFIPAVTEEFSFRLFAIPFFAKIFRSKPLAILIPA
                     LIWGFAHANYPNQPFWIRGAEVSLFGIFVGVLFWRYGILTVLVWHYTVDAIYTAVFLV
                     KTGQPYLIVTAILAAGVIVLPIIYNIVCYRRNRSFADSSGLLNGETVFIIKPEKTITN
                     EVPEISEVKEYQSYSPRKIRNGLIISALFLLILLIPGHRVGDFYRFPIPKNEIRHTAQ
                     EFLAAKEVVPSNFRIAIGLDNNYDPLMGQYVIQHSSLNQLNFIMAQFLPDIITWQVRF
                     FQPNQREEYRIYVHPMKNTVAAFSHILDETDPGARLSKASAQRKAEEFLTFKKYELSN
                     FSLIESNSDVLPNRTDYHFIWESRDEHPASVADARLRLTVTLKGDEIAAFATDYKIPE
                     DWLLAQTRQTTFQSIMTGVQIALIILILYRIIKILLNRPKKVFSGWRIALRLALYLVG
                     LTIIVSGLNYSAALLNYDTSWTLTNWNFLWILSVLLKAIGVGLLTVFIFLTVGFLYPD
                     DIQTLSKAHRPRYTRDALIASILIICGLLACNRLSNYIISMIKPELLANHQITSGLED
                     SLFPFFGALQRIIIMSLLSVATIAFIGFILQNLKGQNFRKVMTLIILTAVFLPDNYLT
                     LSGYLAVYLQFILPILWLYFCLKYFVGANPPAYLYAAVGYFMINQIFSLLRAGSPQGR
                     LAAILLILLGLILSSWLIIEKQNVNFKSTMSN"
     gene            5248..>6116
                     /locus_tag="PLO58_08185"
     CDS             5248..>6116
                     /locus_tag="PLO58_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQO74807.1"
                     /translation="MENTSGIDIGYQFSDKLTDDQTLATEINNASPAVQHFIADLCDK
                     IDNLSKIGAALSAEHNLPVLLQMILEEARRFTNADGGTLYLMSDDRKWLNFAIVQTGS
                     LNIKMGGTSGNPINWKPVPLYLPDGKPNHAMVSAYVALTGEIINIPDVYNVKGFDFTG
                     TRAFDKNTGYRSKSMLVVPLRNYEDEIIGVLQILNAQDRRTGEVCPFSSTDQKLIISL
                     ASQAAIAITNARLIHDLENLFESFIQVIASAIDEKSPYTGGHIERVAQLTMLIAHKVN
                     DVNYGKYADFFMN"
     assembly_gap    6117..6216
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6217..6648
                     /locus_tag="PLO58_08190"
     CDS             <6217..6648
                     /locus_tag="PLO58_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQO74808.1"
                     /translation="RGTLNDAERQIINNHVNVTIKMLEKLPYPKKLCRVPEIAGSHHE
                     KLDGSGYPNHIKDERLSVQAKILALADIFEALTARDRPYKKGKTLSEAMKIMNFMACD
                     RHIDPELFQVFVKEKIYLDYARKFMDPSQIDEVNLDFSVMK"
     gene            6666..>8078
                     /gene="rlmD"
                     /locus_tag="PLO58_08195"
     CDS             6666..>8078
                     /gene="rlmD"
                     /locus_tag="PLO58_08195"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HQO74809.1"
                     /translation="MSYPLKRNQMLELTIEDLAFGGQGIARLNDFVIFFPRSGTPGQR
                     VRARLKRVKENYAEAQIESVLEKSPLEITAPCPYFNYCGGCRFQNLPYEQQIKFLERQ
                     VIELYCHLGGYQDVRIDPIIPAVEIFRYRNKMEFAFSDRRWLVENDDLSRPIDFAIGL
                     RTPDNYYKAVDIDDCLIAPPETTEIIRLVREYARHHNLTPYNHRHHTGYLRHLVLRKG
                     IFTDQIMVNLVTSADTPEILEPLAYNLTQNLKNITSVVNTVTDNVCGTTTGKVIRLLT
                     GQAFITDRIGNLTFKISPASFFQTNTRMAIKLYEIIKQFAQPEPHHIIWDLFCGTGSI
                     ALFLARDCRQVIGFEVVADAVADAIENAQLNVIENALFICADLEKDVLAMPDSVKDLP
                     RPDIIIVDPPRTGLTPQLIATMRQLLPNRIIYVSCNPATQVRDLKKLTADNMYQIIKV
                     QPIDLFPHTPHIETVTLLERY"
BASE COUNT         2178 a         1711 c         1616 g         2373 t
ORIGIN      
        1 acaggaaggt gagccatttt tttgtctttt gccacacttt ttgagatgct tctgttattc
       61 agatatcaaa tggtttcacc cataataacc cttaaaaaaa tattcgcgct cttttaaaat
      121 attttggcta ttacaatcat actttttaat cacttgtgtc tttggtcata aaccagtcct
      181 cactctctaa aaatagtttg aaaatagacg aagatttact aatatttgcc ataaattgcg
      241 aagctgatgt ggcactagta aaaattttac cctcattcta ccaacaattt ttaactcaaa
      301 atagtggaaa taatataacg tgaaagtatt gacgcttaat tcaggtagtt cgtctgtcaa
      361 atatcaattt attgatacca gcactcgaac cgtactttgc aaaggattag ttgaccgaat
      421 tggcataagc ggcactagtc ttactcataa acgccaggat ggtcaccagg tcgttatcac
      481 tgataacatt gttgatcatc aaacggcgat taattctatt atggacattt taatagatcc
      541 tacccacggc gtagcccaga gtcttaaaga aattgaagca gttggtcatc gtttagtgca
      601 cgccggggct agttttaagc agtcagttct aattaatgag gctgttcgcg aaaagttgta
      661 cgaatgtatc gaattcgccc ctctgcataa tccacacaac attaaaggta tcgaggcaag
      721 tttgaaaaaa ctgccgcaca ctcctaatgt aggagtcttt gatactacct tccatatcaa
      781 tatgccagac tatgcttatc tgtacgccat tccttatagc tacaatgaaa aatatgggat
      841 gcgacgctat ggatttcacg gaacatcgca ttattacgtt gctaaccgag ctgctgaaat
      901 cattggaaaa cctattgaac aattaaatat aatcacctgc cacctgggca acggttgtag
      961 cattgcggcg gtcagaggtg gtatttcggt tgatacttcg atgggcttta ctccgctgga
     1021 aggtttagta atgggtacac gttgtggtga tatagatcct ggattgatct tgactattat
     1081 cgatcgtgaa aatttttcta tagaagaggc taatactttt ttcaataaac agtgtggtat
     1141 gaaaggactt tccgggcttt ccagtgatat gcgtgaaata gaaaatgcgg cggccgccgg
     1201 gcatcaacgc gctcgccagg cgctggatat tttttgttac cgcgtcaaaa agtatatctc
     1261 cgcttatatc ggagtcctta atggggccga cctgattgtt ttcaccggcg gcataggtga
     1321 aaattctgcc cttgtgcgag aaaaatctct atctaacctt gaaaatctgg gcatcttgct
     1381 tgatccggca cataataaga ttgcccatgg cgtcgaaggt attatcagca gtgcagaatc
     1441 aaaaattaat gtcctggcag tcccgactaa tgaggagttg gtgattgctt tagagactga
     1501 aaaagtcata atcaccagtc aatgaagcca atttttccac tttttttaca ttaaatgccg
     1561 aaaaaaatgc atttagacgg aagattttct tgacatttta ctttttgtcc cttaacattt
     1621 acccataaat gttgtaaaca aactttagga ggaaattact atggcagaaa aagtnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaaga ctccgtttca acggagtctt
     1801 ttgttttata taaaatggaa ttattttaaa acaagttttt tagaacacta tgagtgaaaa
     1861 taaactgacg cgcaaagatt ggaacctgct ggtgatctgc ggagcgttgc tcctgatttc
     1921 aattatttat ctcagccgca attatcgcaa agcttttccg gcttatgacc ttcaatttga
     1981 tataacacgg gctgattcgc gtgatttagc tgaaaaattc cttagtcacc aacatattga
     2041 tttgaacgac tatcgctatg cggtggtttt cgattatgat gaattgccta agacttttat
     2101 cgaaaaagaa gttggttttg aagaagccac gcgcttgcta tgtcaggact tccccatctg
     2161 gcactggtca aatcgctggt ttaagccgct ttcaccggaa gaaatcaagg tcaatctcgc
     2221 gttagacggc agggtaactt actttgagca ttttttgccg gaagaagcgg cggcagagcg
     2281 catttcagtt gaagcggcac gccggatttg ccaggatttt ctaatgacaa ccatgcaaat
     2341 gaacccggcc gagtgggagt tcattgagga taaaaccgac atcaagccca atcgcgtcga
     2401 tcattttttt acttaccaaa agaagggggt tgaaatttcc agtgcgactt accggcttga
     2461 gatcggcata gtgggaggca aggtcggcaa atatcgtgaa tactggaagt tacccgaaca
     2521 atggttgagc aattataaac acctgcgttc gctgaactcg actacggctt tagccgccga
     2581 tgttttattc gtattaattc tgatagcggc attggttgtt tttataattc aacttagccg
     2641 gcaaaatgtt cacctcaaga cagcgctcgg gtttggcttg attactttta tactacaatt
     2701 tgctggtaac atcaacgaat tgccgattac aatttatgcc ttcgatacca accagtcact
     2761 cgggaattat tatggaaatt atttcattca gacttttgta atcgccctgc tttatgccct
     2821 cctggtggca gtaatcactg gagccggtga agtgctttat cgtcgtgctt atccccaaca
     2881 tctttctatg agtaagctat ttagtccaac cggtttacgc accaaatcat ttttaatcaa
     2941 ttcaactctg gggatgatcc tggcggttgt tttcattgct tttcaaacgg ctttctatct
     3001 cattgccaat cgtttcgggg cttgggcgcc agtcgaggta aattattcca atgctattaa
     3061 tacctatttc ccatggattt ttgtcctact gatcggattt attcccgccg taacggagga
     3121 attttccttt cgtctttttg ctataccgtt tttcgcgaaa atttttcgct ctaagccact
     3181 ggcgatccta atcccggcgc tcatttgggg atttgctcat gccaattatc ccaaccaacc
     3241 attctggatt cgaggagccg aggttagcct ttttggcatt tttgtgggtg ttttattttg
     3301 gcgatatgga attttgaccg ttctagtttg gcattatacg gttgatgcca tttacaccgc
     3361 tgtattttta gttaaaactg gccagccgta tctgatcgta accgctattt tagcggcggg
     3421 cgtgatagtg ctacctatca tttataacat tgtttgctac cggcgcaatc gtagtttcgc
     3481 tgattcgtcc ggcttgctta acggtgaaac agtgtttatt atcaagccag aaaaaaccat
     3541 tactaatgaa gtccccgaaa tttcggaagt gaaagagtac cagagctact ctccgcgtaa
     3601 aatccgcaac ggtttaataa taagcgcact atttttatta attctactaa taccaggtca
     3661 cagagttggt gatttttatc gctttccgat accgaaaaat gaaattaggc ataccgctca
     3721 agaattcctg gccgcaaaag aggttgtgcc gagcaatttt agaattgcta tagggctgga
     3781 taataattat gatccattga tgggtcagta tgtaatccag cattcttcct tgaaccagct
     3841 caatttcata atggctcaat ttttacccga tataattacc tggcaagtgc gttttttcca
     3901 gcccaaccaa cgagaggaat atcgcattta cgttcaccca atgaaaaata cggtggcggc
     3961 attcagccat atcttggatg aaaccgaccc cggggcgaga ttgtcaaaag ctagtgccca
     4021 gcgcaaagct gaggaatttt taaccttcaa aaaatatgaa ctgagtaatt tttcactaat
     4081 tgaatcgaat tcggatgttt tgcccaaccg taccgattat cattttatct gggagagtcg
     4141 ggatgagcat ccggcgagtg tcgctgacgc ccgtttacga cttacggtaa ccctcaaagg
     4201 tgacgaaatt gcggctttcg ccaccgatta taaaattccc gaggattggc ttttggctca
     4261 aacgcgccaa acgacttttc aatccataat gaccggtgtg caaattgctc tgattatcct
     4321 aatactttat agaattatca agatcctatt aaatagacct aaaaaggttt tcagcggttg
     4381 gcggattgcc ctccggttgg cgctctatct tgtcggcttg acaattatcg ttagcgggtt
     4441 aaattactcg gcggctttgc taaattacga caccagctgg acgctgacta actggaattt
     4501 tctgtggatt ttatcagttt tgttgaaagc aatcggggtt ggtttattga ccgttttcat
     4561 ttttttaact gttgggtttt tatacccgga tgatattcaa actttatcga aagcgcatcg
     4621 gccgcgatac acgcgcgacg ctctgattgc ttccattttg atcatttgtg gacttttagc
     4681 ctgtaatcgc ttatcaaact acattatctc aatgataaag cctgagcttc tcgccaatca
     4741 tcaaattaca tccggtctgg aagattcgct cttcccattt ttcggtgctt tacagagaat
     4801 aataatcatg tcactcctgt cggttgccac catcgccttt atcgggttca tattacaaaa
     4861 cctaaaaggc cagaacttcc ggaaagtaat gactcttatt attctgaccg ctgtttttct
     4921 accagataat tacctgacac tttccggtta tcttgccgtt tacctacaat tcattttgcc
     4981 gatcctatgg ctatattttt gcctgaagta tttcgtcggt gccaatccac cggcatatct
     5041 ctacgcggcg gttggttatt ttatgattaa tcaaattttc agcctgctaa gggcaggttc
     5101 acctcaaggt cgtctggcgg ctattttgtt aatcctgctg ggattgattt tgagttcgtg
     5161 gctaattatc gaaaaacaga atgtaaattt taaatcaaca atgtcgaatt gaactttatg
     5221 aaaacacaat gagtatgagg gtataaaatg gaaaacacct ctggaattga cataggttat
     5281 caattctccg ataaattaac cgatgatcag actttggcga ctgagattaa caatgcctcg
     5341 ccagcggttc aacattttat cgctgatttg tgtgataaaa ttgataatct atccaaaatc
     5401 ggtgcggcac tatccgccga gcataatctc ccggttctgc tccagatgat tctggaagag
     5461 gcgcgccgct ttaccaatgc cgacggtggc acgctttatc tgatgagtga tgatcgcaaa
     5521 tggctcaatt tcgcgattgt gcagaccggc tcgcttaaca tcaagatggg cggcacttcc
     5581 ggcaacccga tcaactggaa gccagttcct ctatatctgc cggatggtaa acccaaccac
     5641 gctatggttt cggcgtatgt tgcactaacc ggagaaatta tcaatatccc ggacgtttac
     5701 aacgttaagg gttttgactt taccgggaca cgcgcctttg ataagaatac cgggtaccgc
     5761 tccaagtcaa tgctggttgt ccctttacgc aactacgaag atgaaatcat cggcgttctg
     5821 caaatcctca atgctcaaga ccgccgcact ggcgaagttt gccccttctc atctaccgat
     5881 cagaagctga taatttcgct ggcttcacaa gccgcgatag ccattactaa tgcccgccta
     5941 atccatgacc ttgaaaattt atttgaatct ttcatccaag tcattgcctc tgctattgac
     6001 gaaaaatcac cctacaccgg cggtcatatc gaacgtgttg cccaattgac catgcttata
     6061 gctcacaaag tcaacgatgt taattacgga aaatatgccg atttctttat gaatgannnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgtg gaactttgaa cgatgccgaa
     6241 cgccaaatta ttaataatca tgtcaatgtt acgatcaaaa tgttggaaaa attgccctac
     6301 ccgaaaaaac tatgccgcgt gccggaaatc gctggtagtc accatgaaaa actagacggc
     6361 tccggttatc ccaatcatat caaagatgag cggctttccg tgcaagccaa aattttagca
     6421 ctggctgata tttttgaggc gttaactgcc cgtgaccgac catataaaaa aggcaaaact
     6481 ctatctgaag cgatgaaaat aatgaatttt atggcttgcg atcgccacat tgatccggaa
     6541 ctatttcagg ttttcgtcaa ggaaaaaata tatctcgact atgcaagaaa atttatggat
     6601 ccttcacaaa ttgatgaggt aaacctcgat ttttcagtaa tgaaataaaa tcaggatttt
     6661 cacttttgag ttatccttta aaacgcaatc agatgctgga attgactatc gaagacctgg
     6721 ctttcggcgg tcaggggatt gctcggctga atgactttgt catctttttc ccacgcagtg
     6781 gcacgccagg tcaacgggtg agggctcgcc ttaaacgggt caaggaaaat tacgccgaag
     6841 cccagatcga atcggttctg gaaaaaagcc ccttagaaat aacagcgccc tgcccgtatt
     6901 ttaattactg tggcggatgt cgttttcaga atttgcctta tgaacagcag atcaaattcc
     6961 tggaacgtca ggttattgaa ctttattgcc atctcggcgg ctatcaggat gtccggattg
     7021 atccgattat tccggctgta gaaatttttc gctatcgcaa taaaatggaa tttgcttttt
     7081 ctgaccggcg ctggttagtc gagaacgatg acttgagtcg gccaattgat tttgctatcg
     7141 gtcttcgcac cccggataat tattacaaag ccgttgatat tgacgattgt ctaattgcac
     7201 caccggaaac aaccgagatt attcgactgg ttcgcgagta tgctcgccac cataatttaa
     7261 cgccatataa tcaccggcat cacaccggct acttgcgaca tttagtattg cgtaaaggca
     7321 ttttcactga tcaaattatg gtaaatttgg tgacttctgc cgatacgccg gaaattctcg
     7381 agccgcttgc gtataatcta actcaaaatc tgaaaaacat cacctcggta gttaatactg
     7441 ttaccgataa tgtttgtggt acgaccaccg gtaaagttat tcgtcttctg accggtcagg
     7501 cattcatcac cgaccgaatt ggtaacttga cttttaaaat ttctccagct tccttctttc
     7561 aaacaaatac ccgaatggca atcaaacttt atgagattat taagcaattt gctcaaccgg
     7621 agcctcacca tataatctgg gatttatttt gtggcacagg tagcatcgct ctttttttag
     7681 cacgcgattg ccgccaggtg atcggtttcg aggttgtggc ggacgcagtt gccgacgcaa
     7741 ttgaaaatgc ccaactcaat gtaattgaaa atgctttatt catttgtgcc gatctcgaaa
     7801 aagacgtcct cgctatgccc gattcagtaa aagatttacc gagaccagac atcattatcg
     7861 ttgatccacc ccgaactgga ttaacgcccc aactcatcgc aacaatgcgt caactcctgc
     7921 cgaacagaat aatatacgtt tcctgtaatc cagctacgca agttcgtgat ttaaaaaagc
     7981 taacggctga taatatgtat caaattatta aagttcaacc aatagattta tttccgcata
     8041 ccccgcatat cgaaactgta actttattag agcgatac
//
LOCUS       DAOMJU010000092         8048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_9452_length_8048_cov_19.112974, whole genome shotgun sequence.
ACCESSION   DAOMJU010000092 DAOMJU010000000
VERSION     DAOMJU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9452_length_8048_cov_19.112974"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2296)
                     /locus_tag="PLO58_08200"
     CDS             complement(<1..2296)
                     /locus_tag="PLO58_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQO74810.1"
                     /translation="MKTTRFHSCLRLIVLPLLICGLFNCTQRNVLNKPPEIIEFVRID
                     SGCSTSLSLSEIFTAKKYKPIFSPNPDIQLVYQPDSNIIILTPNNSFSGLTMINFTNQ
                     KKDLCLPIIVKQKNLVTFSYKPPKPVREIFVMGNFNDWNRSSLPMSDEDSDGIFTRDV
                     WLDDGLYEYQFVVDQNEIYDPANPEKTDNGFGYFNSVLRVTAPLADYAANLYILPTGQ
                     NDTISLAVDCLGDTSDIRYFVLLDNHLCPPKSYHHKNKQLQIYLNSLENTIGMHILRA
                     TAQYKNQPGNVITIYLKNGKPLDNNTFIWNDACLYAIMTDRFKNGNSGNDRPVVHPHL
                     ASQANFKGGDFDGIRRTIESGYFDDLGINTLWISPVNKTTDSAYQEWPEPHNYFSGYH
                     GYWPTDARQTDSRFGSLQELKKLVEVAHRHNIKVLLDFVSNHVHIEHPYFREHREWFG
                     QLELPDGSLNIRRWDEYRLTTWFDTFLPSFDYEGSHEALQTMTDNAVWWLKQTDADGF
                     RHDATKHVPHAFWQTLTSKINKRINPHRNLDIFQIGECFGSNELIKSYVNNGMLESQF
                     NFNQFFTARRVFVEPTGDFRDLELAIKKALEVYGYNHLMGNLMDSHDQVRIMAFLDGD
                     LTLSDNGTMRAWQEPPITVDNPLTYRKELVFLGYILTVPGIPVIDYGDEFGLTGANDP
                     DNRRMMRFGEELSDLEREQLQQVSNLIQLRNMHPALRRGDYLPLKAEKDIFIYSRGDA
                     NERLIIALNKSPDIQNPIIFLPDWL"
     gene            complement(2325..>4079)
                     /gene="pulA"
                     /locus_tag="PLO58_08205"
     CDS             complement(2325..>4079)
                     /gene="pulA"
                     /locus_tag="PLO58_08205"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HQO74811.1"
                     /translation="HIYDQPQGGKPTKYKLTKIQNSLWSVTVKGDLKGKYYVYSVAGP
                     DPAYEPKKEVLDPNTTCATTHDGRALIFEENTQIAPAPKFLLKDAIIYEMHVRDFTIA
                     ENSGVKNKGKYLGFTEEGTTIPGTSISTGLDHLTELGINTIQLLPIQDFEHDNKVNNY
                     FWGYMTVNFNSPDGWYTTNENDESRVREVKQMVSALHKRGIKVVMDVVYNHTAETNPQ
                     IRYNFNGIVPSFYYRQREDGSYWNGSGCGNEVRSENPMVRRFIIESLKYWVKTYDIDG
                     FRFDLMGLIGIETMRQIVKELTALKPDIFLYGEPWIVGETPIKPILKGAQREEGFAVF
                     NDNFRDALKGPWHNTDPGYVQTGKNIEAVKIGIRGSIDDFAADPIETINYVSCHDGRT
                     LWDQLVASTGNNKMYTDTQLKAMDKLAAFIIFTSQGIPFMQGGEEFLRTKFGSNNSYN
                     QPDKINKIRWDYKQENHDIFAYYQGLIRIRRDHPMFRMETAEEVRRNLAFFEELGLSV
                     PPNCIAYRLQRGTSGDIWREVIILINPNSRPETFKIPKAKWILAVDHTYADPEIISPI
                     SGPLVTVKPISAMLIYRL"
     assembly_gap    4080..4179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4180..4664)
                     /locus_tag="PLO58_08210"
     CDS             complement(<4180..4664)
                     /locus_tag="PLO58_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HQO74812.1"
                     /translation="MKNYLNNLKTGLIIFGLIASMITSIPKGANEMQTLTVHYHRYDG
                     AYQNWTLWTWSENFSTEIKATGQDDYGLIFNLDLSIYPNPKSIGLLPKYGSWERKDDP
                     DRYWKPNSHHEVWLLQGDETVYNSRPDISPKIRRAFLDSPTTITLVFSNAITKEMSTS
                     LA"
     gene            4889..5722
                     /locus_tag="PLO58_08215"
     CDS             4889..5722
                     /locus_tag="PLO58_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HQO74813.1"
                     /translation="MKKQTRKLTILFLCFLIWAVGLFSDAPEPVPKTSDPAQNVVRIG
                     VLKLPAVLVKGGTFEMGSDDESSPEDTRPAHTVTLTDFYISVTEVTVGQYKEYCRQTG
                     KAMPEQEAGTNDSYPIAFVTWYEANEFCQWVGGYLPTEAQWEYAARSRGLNLKYPNGN
                     TIDHSFANYSGTGQTDKWKRVAPVAKFPPNSLGLYDMAGNLYEWCQDYYRSDYYKNSS
                     QINPTGPASGMFKVIRGGSWYHGKEAMRTTSRYRYMPVARLSFLGFRVAWDPDAVRVS
                     E"
     gene            5769..7967
                     /locus_tag="PLO58_08220"
     CDS             5769..7967
                     /locus_tag="PLO58_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74814.1"
                     /translation="MRRDYVKFAIMPAVMGAVAMIAQVAYLRLTAQSFYGNELTMCIA
                     LGHWLIWTGLGSLVGARLVRRNLSEKWLTAIVLSYGMVLIIFAGLLFLNRRIAGIPAG
                     TVVGLGTIFAWTFILFSLPAWLNGLFFPLIVNWVKVKAELSPVHTVYAGEVIGSAWGS
                     LLFAGLILSGVSTIDCLLVIVWVMMVLAIGLFIYSERMRIFLTIGASLIVILASVGFR
                     GPITNWRWAPFRALLSRESPHLALTTVRYQDAISVYGDSQPLWTFGDREKAEEWTHFA
                     LLNHPDPRSVLVIGIGSIEICREISRHPSVERITILQPDRILHQMVTQFGSIDALDTN
                     VQVIFADPALYLGKTDERFDVVLMNIPLPVNAQWNRFYTREFFEIVHSGLSPGGLLML
                     NLPGDEEFLPPAQVEFLKTMQNTANRVFQETTWIPGLTVHLLASDSTLHNDRAFFLDG
                     LRSRNLEPIYISDYFLHDRLSDWKIDFLTNRISEAEIQEVNTIARPIGYYYDTVLWGQ
                     RTGGIVKKVYPFLRELGFIPILIGFIVVILIVRPISHRKKIDPAVRMAGIGFWVMGLE
                     SVAIILFQSLVGSVYLWIVFLTFAYMLGSGLGAIMQLKWKRADIILERVGFLLMLILP
                     FLLALPLTGGWPILLVTLWIGLILFIGGVVAGCIFPLMVKASTTQADKIANSAGLIYA
                     ADIFGSTLGTYLISGIVIPVYGIIPALGIVVAVELGIGAFNFPVTHLTEK"
BASE COUNT         2076 a         1587 c         1912 g         2373 t
ORIGIN      
        1 ttaaccaatc cggtaaaaat attatcggat tttgaatatc cgggctttta tttaatgcga
       61 taataagccg ttcattggca tcgccacggc tgtaaatgaa aatgtccttt tcggctttca
      121 gtggtaaata atcgccgcgt cgtaaagccg gatgcatatt cctcaattgg attaaattgc
      181 ttacctgctg taattgttct cgttccagat cagataattc ttcaccaaaa cgcatcatac
      241 gacgattgtc cggatcgtta gcgccggtca agccgaattc atctccgtaa tcaataaccg
      301 gaattccggg tacagtcaat atatatccga ggaagactaa ttctttccgg taagtaagcg
      361 gattatccac cgttatgggc ggttcttgcc aggcgcgcat cgtgccgttg tcagacaaag
      421 tcaagtcccc atccaaaaaa gccattatgc gcacctgatc gtgactatcc attaaattgc
      481 ccatcaggtg attgtatcca taaacttcca acgccttttt aatagccagc tccaaatcgc
      541 gaaagtcgcc ggtcggttcc acgaaaaccc gacgagcagt gaaaaattga ttaaaattaa
      601 attgcgattc gagcattcca ttattaacat aagatttaat taattcatta ctgccgaaac
      661 attcaccgat ttgaaaaata tccagattcc ggtgaggatt gatccgctta ttaattttgg
      721 atgtcagagt ttgccagaaa gcatggggca catgtttggt agcatcgtgt cgaaaaccgt
      781 ccgcatcagt ttgctttagc caccagacag cgttgtcagt catagtttga agggcctcgt
      841 gcgagccttc gtaatcaaaa gacggtagaa aagtatcgaa ccaggttgtc aggcgatatt
      901 cgtcccagcg ccggatattc agagatccgt ctggcaactc caattgaccg aaccactcac
      961 gatgttctct gaaataggga tgctcgatat ggacatggtt ggagacaaaa tcaagtaaaa
     1021 ctttgatatt atgacgatga gccacctcga ctaatttttt taattcttgg agggagccaa
     1081 aacgcgagtc agtctgtcta gcatcagttg gccagtaacc atgataaccg gaaaagtaat
     1141 tatggggctc aggccattcc tgataagcac tatcagtggt tttattgacc ggtgaaatcc
     1201 agagagtatt gatgccgagg tcgtcgaaat aaccggattc gatagtccgg cggattccgt
     1261 cgaaatcacc gcccttgaaa tttgcttgtg acgctaagtg agggtgaact accggtcggt
     1321 cgtttcctga gttgccgttt ttaaaacggt cggtcattat ggcgtaaaga caagcatcgt
     1381 tccaaatgaa ggtattatta tcgagtggtt taccattttt caaatagata gtgatcacat
     1441 tgccgggttg atttttgtat tgagccgttg cccgcaaaat atgcatccca atcgtatttt
     1501 caagtgaatt taaatagatc tgcagttgtt tatttttatg atgatagctt ttgggcggac
     1561 aaagatgatt atccagcaag acgaaataac ggatatcgct ggtatcaccg aggcagtcaa
     1621 cagctaacga aatagtatcg ttctgtccgg ttggcaaaat atagagattg gcggcataat
     1681 cagccagcgg agccgtaacg cgtaaaaccg aattgaaata gccgaatcca ttatcggtct
     1741 tttccggatt ggcgggatca taaatctcgt tctggtcaac aacgaactgg tattcgtata
     1801 aaccatcgtc gagccaaaca tcccgtgtga aaattccgtc actgtcttca tcagacattg
     1861 gtaatgagct ccgattccaa tcattaaaat tacccattac gaatatttcc cgaaccggtt
     1921 tgggaggctt ataggaaaag gtgaccagat tcttttgttt gacaatgatc ggcagacaaa
     1981 gatctttttt ctggttggta aaattaatca tagtcaatcc ggaaaatgag ttgttcggag
     2041 tcagaataat tatattggaa tcgggttgat agactaattg aatatccggg ttgggactga
     2101 aaatcggttt atatttcttt gcggtaaata tttcagagag actcaatgat gtactacatc
     2161 cggaatcaat tcgaacaaat tcgattattt ccggtggttt atttaatacg ttccgttggg
     2221 tacagttaaa gagaccgcat atcagcaagg gtaaaacaat taagcgtaaa caagaatgaa
     2281 atcgagttgt tttcatctag ttctttaggg aggattacct gtaattaaag gcggtaaatc
     2341 agcattgccg aaatgggttt aacggtaact aaaggtccac taatcgggga aataatttcc
     2401 ggatcggcat aagtatgatc tacagccaga atccatttag cttttgggat tttaaaggtt
     2461 tccggacggc tgttcggatt gattaggata ataacttcgc gccagatgtc gccgctggta
     2521 ccgcgctgga gacgataggc tatgcaattg ggtggtacgc tgagtccgag ttcttcaaaa
     2581 aatgccaaat tgcggcgcac ttcttcggcg gtttccattc ggaacattgg gtggtctctg
     2641 cggatacgga ttagtccctg ataataggcg aaaatgtcat gattttcctg tttgtaatcc
     2701 cagcgaatct tattgatttt gtctggctgg ttgtagctat tattggaacc gaatttggtg
     2761 cgcaggaatt cctcaccgcc ttgcatgaaa ggaataccct gcgaggtgaa aatgataaaa
     2821 gcggctagct tatccattgc ttttagttgc gtgtcggtat acattttatt gttgccggtg
     2881 gaggcaacta attgatccca gagtgtgcgg ccgtcgtggc aggaaacata attaatggtt
     2941 tcgatcggat cagcagcaaa atcgtcgata ctaccccgaa tgccgatttt aacggcttcg
     3001 atatttttgc cagtttgaac gtatcccgga tcagtattat gccacggtcc tttgagagcg
     3061 tcacggaagt tgtcattaaa tacggcgaag ccttcttcgc gttgagcgcc tttcaaaatc
     3121 ggtttgatgg gcgtttcacc gacaatccag ggttcgccat agagaaaaat gtcgggctta
     3181 agggcggtta gttctttaac gatttggcgc atagtttcta tgccaattaa tcccattaaa
     3241 tcgaagcgaa atccgtcaat gtcataagtc ttgacccagt atttgagtga ttcgatgata
     3301 aagcgacgta ccatcggatt ttcactgcgc acttcattgc cgcatccgga accgttccaa
     3361 taagagccgt cctctctctg gcgatagtaa aagctcggca caatcccatt aaaattataa
     3421 cggatttgcg gattggtttc ggcggtatga ttgtaaacta catccataac gactttgatg
     3481 ccgcgtttgt gaagggcaga aaccatctgt ttgacctcgc gcacccggct ctcgtcattt
     3541 tcgttggtag tgtaccaacc gtccggcgaa ttaaaattga ccgtcatata accccagaag
     3601 taattgttaa ccttgttgtc atgttcgaaa tcttgaatgg ggagcaattg gatggtgttg
     3661 attcccagtt cagttaaatg gtccagaccg gtgctgattg aagtgccggg aatggttgtg
     3721 ccttcttcgg tgaaaccgag gtatttgcct ttgttcttta caccggaatt ttcggcgata
     3781 gtaaagtcgc ggacgtgcat ttcatagata attgcatctt ttaggaggaa tttaggtgca
     3841 ggcgctatct gagtattttc ctcgaaaatc aaagcccgtc catcgtgagt agtagcgcag
     3901 gtggtattag ggtcgagaac ctcttttttc ggttcataag ccggatcggg accggcgacg
     3961 ctataaacat aatatttccc tttcagatca cctttcaccg ttacagacca gaggctgttt
     4021 tggatcttgg tcaatttata tttggtaggc ttgccacctt gcggttgatc ataaatatgn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgagggaagt tgacatttct
     4201 ttggtaattg cattggagaa gacgagcgta atggtggtag gactatcgag aaaagcccgg
     4261 cggattttgg ggcttatatc aggacgagag ttatagactg tttcatcacc ctgtaagagc
     4321 cagacttcgt gatgggaatt cggtttccag taacgatccg gatcatcttt tctttcccaa
     4381 ctgccatact ttggtaaaag accgatactt ttgggattgg gataaatact cagatcgagg
     4441 ttaaaaatca gaccatagtc atcctgaccg gtcgctttga tctcagtgct gaagttttca
     4501 gaccaggtcc agagcgtcca attctggtaa gctccatcgt agcggtgata atgaacagtg
     4561 agggtttgca tttcgttagc gcctttcggt atagaggtga tcatactggc aatcaatcca
     4621 aatatgatca atccggtttt taaattgttt aggtagttct tcattgaacg attccttttt
     4681 aggaatgatt aacatatcca atattaacaa actttgtttt taaataacat aaaaaattgc
     4741 catttctaaa taattctgac gatatgtgga attttatcgt gtttttaaag ctcatttaag
     4801 tcgtcaaaag cgagtacgag ataaaaagtc cttgatattc cgaagttctt tttatagttt
     4861 aattatagat ttagggatgt aataggaaat gaaaaagcaa acgcgaaagt taacaattct
     4921 ttttctgtgc tttctaattt gggctgtcgg gctttttagt gatgctcctg aaccggttcc
     4981 aaagacgagt gacccggccc agaatgtggt aagaattgga gttttaaagc taccagcggt
     5041 gctggtcaag ggtgggactt tcgaaatggg ctctgatgat gaaagtagtc ccgaggatac
     5101 aagaccggct cataccgtca cgctgactga tttttacatt agtgtaacgg aggtgactgt
     5161 cggtcaatac aaagagtatt gtcgtcaaac aggtaaagct atgccggaac aggaagcggg
     5221 aactaatgat tcttacccga ttgcatttgt tacctggtat gaagccaacg aattttgtca
     5281 gtgggttggg ggttatttgc ctactgaggc gcaatgggag tatgccgctc gttcgagggg
     5341 tttgaacttg aaatacccga atggcaatac tattgatcat tcatttgcta attattccgg
     5401 aaccggtcaa actgacaaat ggaaaagagt tgcgccggtg gcaaaatttc caccgaatag
     5461 cctcggtctt tatgatatgg ctggcaatct ttatgaatgg tgtcaagatt attaccgcag
     5521 tgattactac aaaaattcca gccaaataaa tcccacgggg ccagcttccg gtatgttcaa
     5581 agttatccgg ggaggaagtt ggtatcatgg caaagaagca atgcgaacga ccagtcgtta
     5641 tcgctatatg ccggttgctc ggctaagttt tctcggattc agagtcgcct gggatcctga
     5701 cgcggtccga gtttcggaat agtgtttact aataaaattt accttaagat tttcgcatag
     5761 gcatttttat gaggcgagat tacgtaaaat ttgccataat gcctgctgtg atgggcgcgg
     5821 ttgctatgat tgcgcaggtt gcatatttgc gcctgacggc tcagtcattt tacggcaatg
     5881 aactgacgat gtgcattgca ctcggtcact ggttaatctg gactgggctg ggtagtttgg
     5941 taggcgcgcg attagtgcgg cgcaatttat cagaaaaatg gttgaccgca atcgttctaa
     6001 gctacggaat ggttctgatt atttttgccg gactgttatt tttaaatcgc cggattgccg
     6061 ggattcccgc cggcacagta gtcggcttgg gaacgatttt cgcctggact tttatccttt
     6121 tcagtctgcc agcctggttg aatggattgt tttttccgct aatcgtgaat tgggtcaaag
     6181 ttaaagcgga gctttccccg gttcatacag tttacgctgg tgaagtaatc ggctcggctt
     6241 ggggaagttt gctttttgcc ggtttgattc tatcgggagt gagtactata gattgcctgt
     6301 tggtaattgt atgggtaatg atggttttgg cgatcggact ctttatttac tccgaacgaa
     6361 tgcgaatatt tttaaccatt ggggcttcgc tgatagttat tttggcttca gttggtttcc
     6421 gagggccaat tactaactgg agatgggcac cgtttagggc tttgctcagc cgcgaatcgc
     6481 cgcatcttgc gttgactacc gtgcgttacc aggacgccat cagtgtctat ggcgacagtc
     6541 agccgctctg gactttcgga gaccgtgaga aagccgagga gtggacgcat tttgctttgc
     6601 tgaatcatcc tgatccgcga tcggttttag tcattggaat cggaagcatt gaaatctgtc
     6661 gcgaaattag tcggcatccg tcagtcgagc gtattacaat ccttcaacct gaccggattc
     6721 tgcatcaaat ggttactcaa ttcggatcaa ttgatgcgtt agacaccaat gttcaggtca
     6781 ttttcgctga tccagcttta tatttaggga aaactgacga acgattcgat gtggttttaa
     6841 tgaatattcc tctgccagtc aatgcccagt ggaatcgctt ttatactcgg gaatttttcg
     6901 aaatcgtaca tagcggatta agtccgggcg ggcttttgat gctaaattta cctggtgatg
     6961 aggaattttt gccaccggcg caagtcgaat ttctcaaaac tatgcaaaat actgccaacc
     7021 gggttttcca ggaaacaacc tggattcccg gtctgacagt tcacctcctg gcgagcgatt
     7081 caactctaca taacgatcga gcgttttttc tggatggttt gcgaagccgt aacctcgaac
     7141 caatctatat tagcgattat tttctacatg atcggctctc agactggaaa atagattttc
     7201 tgaccaatcg aatttcggag gcggaaattc aagaggtgaa cacaatcgcc aggccgattg
     7261 gctattatta tgataccgtg ctttggggtc aacgcaccgg cgggatcgtt aaaaaagtat
     7321 atccgttttt gcgggaactg ggctttatcc cgattctgat tggttttata gtggtgattt
     7381 taatcgttag accgatttcc catcgaaaaa aaatagaccc ggcggtgcga atggcgggga
     7441 ttggtttctg ggtgatgggc ttggaatcag ttgccattat cctttttcag agtttagtgg
     7501 gtagtgttta tttgtggatc gtatttttga cttttgctta tatgctgggt tctggtctgg
     7561 gcgcaattat gcaattaaaa tggaagagag cagatattat tctcgagcgg gttggctttt
     7621 tactgatgct aattttaccg tttctcctgg cgttgccgct aaccggtgga tggccgattt
     7681 tgctggtgac gctctggatt ggactaattt tatttatcgg tggagttgtg gctggctgta
     7741 tatttccatt gatggtaaaa gccagcacga cacaagcaga taaaatagcc aattctgccg
     7801 gtctgatcta tgccgccgat atttttggct cgacactcgg aacttatttg atttccggaa
     7861 ttgtcattcc agtatatgga attataccgg cgctgggaat tgtggtggcg gtagaattgg
     7921 gaataggcgc attcaatttt ccggttactc acctgaccga aaaatagccg gataaaggct
     7981 gagtgataag tctctaatta attgtttgtt acttcaccgt ctcacggttg aatacctgag
     8041 ccgagaaa
//
LOCUS       DAOMJU010000093         7977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_9567_length_7977_cov_18.064630, whole genome shotgun sequence.
ACCESSION   DAOMJU010000093 DAOMJU010000000
VERSION     DAOMJU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9567_length_7977_cov_18.064630"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="PLO58_08225"
     CDS             <1..1574
                     /locus_tag="PLO58_08225"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQO74815.1"
                     /translation="FKPVDPNYTIKNYWKGIYTYSTGKTILNNVRVEGANYGLYIYYG
                     RGYVYHCTFYNNNYGLRILYPNSSFNIDRCTFTNNNTAAYSSSANVLVANSTFSNNTY
                     GLKTYSSTGSILSNVLSNNSQGIAIYSGSLDFSTSTKNISYINNTIQNNSQYGIYIGS
                     SAYPTLGEYYVDSKGYTHGGFNLFSRGTGAYDVYSLYASSIDARMNWWASNANRYGNF
                     LIVPTADDGGLLKTVSQYSIENGQDQIRFQETMSQASQLISDSLFNEAISVYRTIISD
                     YPHCLESAFALNGIVYSYYCLQHPEKILSELESLLLTNKGNLVEIFIKDFISTEYIKY
                     ERFIEASNLLDELLIDYEKYEMDEQAAYTLLNKTYLQQYLTNNLSIGKKGGIATQFKS
                     VECLILLYSKYPNSTAASLAKEELGDDFFINEPLPEQTEVIIADHRIYPNPFNINTTV
                     AFDLPEEAGVEIIVYDLMGREVWKSAKINYSAGTYSVIWHGVNQSGQPVGTGMYLVRL
                     NSTKYNATQKVLLMK"
     gene            1589..2020
                     /locus_tag="PLO58_08230"
     CDS             1589..2020
                     /locus_tag="PLO58_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQO74816.1"
                     /translation="MDQFTQFMELMGDKVTQVIEKTRRLYFVVSNENLKEIARYLFVT
                     LGCRLSTATAQETYHGLEILYHFSHDASGCYYCPRVVLTDKKNPKVNTITDIVTGALW
                     IEREIFDLWGIEFSGHPRMEQLLTLNSPENPDRPLRFGRSL"
     gene            2017..>2433
                     /locus_tag="PLO58_08235"
     CDS             2017..>2433
                     /locus_tag="PLO58_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HQO74817.1"
                     /translation="MTENKIPLGPFHPLLEEAEYFELKVDGETVVDIDMEIGWMHRGH
                     EFISESKTFTQSIYLVERICGICSTSHPLACVHAIEDIDNIEIPLRARYIRTLIGELE
                     RIHSHLLWLGLAGHFIGYDTLWMWAWKYREPILEMFE"
     assembly_gap    2434..2533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2700..>4327
                     /locus_tag="PLO58_08240"
     CDS             2700..>4327
                     /locus_tag="PLO58_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQO74818.1"
                     /translation="MPNNLILMVTLPIVIAVLNTFLPTILKKVLTFLGLGYLLYLCYD
                     LFTGAAEAVYLFGQPILGMDKMALFALIFIQVMSFIILVFSLKGVDKGIERGFFILYP
                     MTVGISNGIVLSVNTFGLLIFWGLSGLMLYLFGLLGQTSSAPQTAKKTFIIVGGSDVF
                     LILGLILIWFLGAGNSWSLWNTQIVMSGGLAYVAFFSILIAAFAKAGGFPFHTWLPDF
                     SQDAPLESVAFLPASLDKLVGIYLLARLSVSLFVINLNIQMILISLGALTVICAVMMA
                     LIQHNGRRLLGYHAVSQVGYMIIGVGSGSVIAFIGGLFHMVNHTIYKSTLFLTLNSVE
                     KQTGTSNLDELGGLGRNMPLTFIAALTGALAISGIPPLNGFYSKWLIYQGLIEKMQSL
                     TAGYQIWLLICLILAVFGSALTLASFMKFIHAIFLGKRPKKWKDVKEAPFNQWFSVGV
                     LALLCVVFGLFAKPLPIGKFIAPIISELGLPTPVILGSYEPVQLFLLFGTAFLLGLVI
                     YLLVKNVRFDENYLGGMPSLEKFRIAGTEFYNEI"
     assembly_gap    4328..4427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4428..4958
                     /locus_tag="PLO58_08245"
     CDS             4428..4958
                     /locus_tag="PLO58_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HQO74819.1"
                     /translation="MFILGFMLVGSVAALEIKDLLSAIIAVGIVGLGLSMAFLLLQAP
                     DLAIVQFLFEIFAIVIMVRAFMKPGYHFSDTKSKHRGRTIIVMAVLVAIFILSINVFK
                     ALPPFGEPLMTTSQHYLENAARETGSANIVASIVLDYRGYDTLGEATVLFVAVLGTLT
                     ILRVKSNKKKEEESEK"
     gene            4955..5404
                     /locus_tag="PLO58_08250"
     CDS             4955..5404
                     /locus_tag="PLO58_08250"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HQO74820.1"
                     /translation="MKQEPGLTLIAKTVTRVSVWLIILYGIYIILHGHLSPGGGFAGG
                     VVIALAFLNVMLTYGRETVTHWVNVEFLHDLEPACGAIYMAIGILGISISGGFLANFL
                     SKGTLFNLLSSGNILLLNIIIGIKVAMGLFTVVWTLAIFHINEGGEK"
     gene            5401..5745
                     /locus_tag="PLO58_08255"
     CDS             5401..5745
                     /locus_tag="PLO58_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HQO74821.1"
                     /translation="MIVYLLCFALLLVGIYGLLVKRDLVRLIIASGITGYATNLMLVL
                     FGYKRGAIYPILEEGKAVGERIMVDPLPQALVLTSIVIELGITAMLVALAIRLFEKNK
                     TFDITKIKRLSG"
     gene            5742..>6115
                     /locus_tag="PLO58_08260"
     CDS             5742..>6115
                     /locus_tag="PLO58_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH/ubiquinone/plastoquinone (complex I)"
                     /protein_id="HQO74822.1"
                     /translation="MILPFFIIIPLLTAFIITLIAGERDNWAVILSILAVVSLVILSV
                     YCFVSLNGRTLVYEMGRWKAPIGIILAVDALSAFMLMIVSIIALTAIMFSVQYIRNLS
                     PSWKYYALFMLLTGGMNGVIATG"
     assembly_gap    6116..6215
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6216..6890
                     /locus_tag="PLO58_08265"
     CDS             <6216..6890
                     /locus_tag="PLO58_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQO74823.1"
                     /translation="FSEVFLVLGTVSMVIGVFLALGQWDVKRLLAYHSISQIGYIIIG
                     VGLGTTLGLLGAVFHLLNHAIFKALLFLNAGSLEMALGTRDLRQMGKVSKALPVTSFT
                     SMVASLSISGIPPFNGFFSKLIIFIAAIQIGRPVVAFLAGLGSLLTLASFMKVQRYGF
                     HGEKTVTADQAKVGWGMRAAMIVLAILCLATSALVIPGVREVTLDPVVEVIMNKTGYI
                     TRVLGR"
     gene            6893..7399
                     /locus_tag="PLO58_08270"
     CDS             6893..7399
                     /locus_tag="PLO58_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HQO74824.1"
                     /translation="MTIKNRSRVIVFVLSFLVWLALTDIKDIQEVVAGLIVAFLVSLI
                     AGTFLVTTEKSRHPVRRFFMGIVYLFKFLWEMIKANIHVALIVINPTLPIKPGIIKIK
                     SNLTKDSALTVLTNSITLTPGTLTVDINPETREIYIHCIEVESTAIDENTRSIGGRFE
                     KLIREVFE"
     gene            7396..7734
                     /locus_tag="PLO58_08275"
     CDS             7396..7734
                     /locus_tag="PLO58_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HQO74825.1"
                     /translation="MRVLRWVIGLVLIGAFMYANFFIYTASIYVKLINVILFSALFVI
                     YRVIVGPTAADRIVAVDILGILIIGMLALLGLNYDQSFFMDIGLIWALLSFIASLAFA
                     KVLEGRSLDD"
     gene            7727..>7977
                     /gene="mnhG"
                     /locus_tag="PLO58_08280"
     CDS             7727..>7977
                     /gene="mnhG"
                     /locus_tag="PLO58_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HQO74826.1"
                     /translation="MIRTLIGMAFILIGVTFDFLGVLGLVRLPDVYNRLQAATKCVTF
                     GSAGLLFGVFILQGFNSFGFKVLIGIVFIFLTSPVAAHV"
BASE COUNT         1925 a         1431 c         1851 g         2470 t
ORIGIN      
        1 tttttaaacc cgttgatccc aattacacga tcaagaacta ctggaaaggc atctatacgt
       61 acagtaccgg caaaaccatc ttgaataacg tccgcgtcga gggtgctaat tatggattgt
      121 acatttatta tggtaggggc tatgtgtacc attgcacttt ctacaataat aattatggtc
      181 tacggatttt ataccctaac agttccttta atatagatcg atgtactttt accaataaca
      241 atactgcggc ctattctagt agcgccaatg ttctcgttgc caacagtact tttagcaata
      301 acacttatgg cctgaaaact tatagtagta caggaagtat attaagcaat gtactttcca
      361 ataattcaca aggcatcgca atatattctg gtagtttaga tttttccacc tcgactaaaa
      421 atataagtta tatcaataat acaatccaga ataacagcca gtacggtata tatatcggta
      481 gtagtgccta tcccacactg ggtgaatact atgttgattc gaaaggttat actcatggcg
      541 gattcaattt attttcacga ggtaccggcg cttatgatgt ctatagtctg tatgcatcat
      601 ctattgatgc taggatgaat tggtgggcgt ccaacgctaa tcgatatggt aattttctaa
      661 ttgtacctac ggcagatgat ggcggcttac ttaaaacggt atctcaatat tctatagaaa
      721 atggacaaga tcagatcaga tttcaggaaa ctatgtctca agcgagtcag ttgatttcag
      781 atagtctatt taacgaagca atatctgtct atagaacgat tatttcggat tacccacatt
      841 gcttagagtc agcatttgct ttaaatggta tcgtttatag ttattattgt ctgcaacatc
      901 ccgaaaaaat tctatctgag ttggaatcat tgcttcttac taataaaggt aacctggttg
      961 aaatatttat aaaagatttt atctcaacgg aatatattaa atacgaacga tttattgagg
     1021 catcaaattt acttgatgaa ttgttgattg attatgaaaa atacgaaatg gatgaacagg
     1081 ccgcatatac acttttgaat aaaacttact tacaacagta cctaacaaat aatttatcaa
     1141 tcgggaaaaa aggaggaata gcgacccaat ttaaatcagt agaatgtctg atattacttt
     1201 attccaaata tccaaattct acagccgctt cattggcaaa agaggaattg ggtgatgatt
     1261 ttttcattaa tgaaccttta ccagaacaaa cggaagtaat aatagccgat catcgcatct
     1321 atcctaatcc cttcaatatt aatacaactg ttgcattcga cctgccggaa gaagccgggg
     1381 ttgagatcat cgtttacgac ctgatgggac gggaggtttg gaaatcggcg aagatcaatt
     1441 attccgccgg aacctactcc gttatctggc atggcgtcaa tcaatccggt cagccggttg
     1501 gcaccgggat gtatctcgtc cggctgaatt ccaccaaata taatgccacc caaaaagtct
     1561 tacttatgaa atgaaaatgg agatattgat ggatcagttt acgcaattta tggaattaat
     1621 gggcgataag gttacgcagg taatcgaaaa aacgcgccgg ctctattttg tggtgtcgaa
     1681 tgaaaacctc aaagaaattg cccggtattt gttcgttacg cttggctgtc ggctctcgac
     1741 cgctactgct caagaaacct atcacggtct cgaaatcctg tatcattttt cgcacgacgc
     1801 cagcggttgc tattattgcc cacgggtggt tttaactgac aagaagaatc caaaggttaa
     1861 taccatcacc gatattgtga ccggcgcact gtggatcgaa cgcgaaatat tcgacttatg
     1921 gggtatcgag tttagcgggc atccgcgtat ggaacaactc ctgacgttga atagtccgga
     1981 aaatcctgac agaccattac gcttcgggag gtcgttatga ccgaaaacaa gattccactt
     2041 ggtccatttc atccactatt ggaagaagcc gagtacttcg agctgaaagt ggacggtgag
     2101 acggtggttg atattgatat ggaaatcggc tggatgcacc gcggacatga gttcatcagc
     2161 gaatccaaga cttttaccca atccatttat ctagttgaaa gaatttgtgg catctgctcg
     2221 acttcgcatc cgcttgcctg tgtacatgct atcgaggaca ttgataatat cgaaattcca
     2281 ctgcgagcgc gctatatccg taccttaatt ggcgaactgg aacggattca tagtcattta
     2341 ttgtggttgg ggctggcggg gcattttatc ggatatgata cgctttggat gtgggcttgg
     2401 aaataccgtg agccgattct ggaaatgttt gaannnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnaagggga ttccggttgc cgatgcatta attactttag ccgccgtgga
     2581 tccgtgctac tgctgtacgg agcgggcgct gagagtgatt gatcccctga accggccttg
     2641 tgagattgac ttgctgaagc tttcacgtgc caaaaccgaa caaatccgga gggaaagtta
     2701 tgccgaataa tttgattttg atggtgacat tgccgatcgt tattgcggtg ttgaatacat
     2761 ttctcccaac gattttgaag aaagtgttga cctttctcgg gctgggctat ttgctatatc
     2821 tgtgttacga tttatttacc ggagcggcgg aagcggttta ccttttcggt caaccgattc
     2881 tgggaatgga taaaatggcg ctgtttgccc tgatttttat tcaagtgatg agttttatta
     2941 ttttggtttt ttcactgaag ggcgttgata agggcatcga acgcggattt tttattctgt
     3001 atccaatgac agtcggcatt agtaatggaa ttgttctgag tgtcaatact tttggcttac
     3061 tgattttctg gggactttct ggtttgatgt tgtatttgtt tggtctattg ggacagactt
     3121 ccagcgcgcc gcaaactgcc aagaaaactt ttattatagt cggcggaagt gatgttttcc
     3181 tgattctcgg attgattcta atctggtttc tgggagcggg taattcttgg agtttatgga
     3241 atacgcagat agttatgagc ggaggtctgg cttatgtcgc cttcttcagt attctgattg
     3301 cggcttttgc caaagccggt ggatttccgt ttcatacctg gttgccggat tttagccagg
     3361 atgcgccgct ggaaagcgtg gcttttttac cggcttcgct ggataaattg gtgggaattt
     3421 atctgctggc gcgattgtcc gtttcactat ttgtgattaa tctcaatatt cagatgattt
     3481 taatatcgct gggcgctctc acggtaattt gtgccgtaat gatggcgttg attcagcata
     3541 atggtcggcg cctgcttggt taccatgccg tcagtcaggt cggttatatg attattggag
     3601 ttgggagtgg gagtgtgatt gcttttatcg gcggtttatt tcatatggtt aatcatacga
     3661 tttacaaatc aacacttttc ctgacgctta attcggtaga gaagcagacc ggaacctcta
     3721 atcttgatga attaggaggc ttaggtcgca atatgccgct gacattcatc gcggctttga
     3781 ccggggcgct ggcgatttcg ggaattccgc cgctaaacgg tttttactcg aaatggctga
     3841 tctaccaggg attgatcgaa aaaatgcaga gtttgacagc cggttatcaa atttggctct
     3901 tgatttgcct gatactggcg gtttttggaa gcgcattgac cttagccagc tttatgaagt
     3961 tcattcatgc tatttttctc gggaaacgtc ctaagaaatg gaaagacgtt aaagaggcgc
     4021 ctttcaatca gtggttttcc gtgggtgtat tagccttgtt atgtgttgtt tttggattat
     4081 tcgctaagcc tttgccgatc gggaaattta ttgctccgat aatatccgaa cttggtttgc
     4141 caacgccggt tattcttggg tcatacgaac cggtgcagtt gtttctgctg tttggtacgg
     4201 cattcctgct gggtttagtt atttatctgc tggtcaaaaa tgttcggttt gacgaaaatt
     4261 atcttggcgg gatgccgtct ctggaaaaat tccgcattgc cgggacggag ttttataatg
     4321 aaatcagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt ttcattttag
     4441 gttttatgtt agtgggttcg gtcgcggcgc tcgaaatcaa ggacttgcta tcggctatta
     4501 ttgcggtggg aattgtcggg ttgggactgt ctatggcatt tttgctttta caggcgcccg
     4561 acctggcaat tgtccagttt ctattcgaga tttttgcgat agtgataatg gtgcgagcct
     4621 ttatgaaacc gggatatcat ttcagcgata cgaaatccaa acataggggt agaacaatta
     4681 tcgtaatggc agttctggtt gccattttta tattaagcat caatgttttc aaagccttgc
     4741 cgccctttgg cgagccattg atgaccactt cgcaacacta tttggaaaat gccgccagag
     4801 aaaccggttc agccaatatc gtggcttcga tagttctgga ttaccgcggt tatgatacgc
     4861 tgggagaagc caccgtatta ttcgtagcgg ttttaggaac gctgactatt ctaagggtga
     4921 aatcgaataa gaaaaaagaa gaggaaagcg aaaaatgaaa caagaacctg gtttaacatt
     4981 aatcgccaaa accgtgacgc gggtcagtgt ctggttgatc attctctacg gaatctatat
     5041 aattttgcac ggtcatttga gtcccggtgg cggttttgct ggcggggtgg tgatcgcatt
     5101 agcatttctc aatgtgatgt taacctatgg tcgcgaaacc gttacgcact gggtcaatgt
     5161 cgaatttctg cacgatttgg aaccagcctg cggtgcgata tatatggcga ttggcatttt
     5221 aggaatttct atcagcggcg gttttttagc caatttttta agtaaaggga cgctttttaa
     5281 tctgctcagt tccgggaata ttctattgtt aaatattatt atcgggatca aggtggctat
     5341 gggattattc accgtggtct ggactttggc gattttccat ataaacgagg gaggtgaaaa
     5401 atgatcgttt atctgttatg ttttgcatta ttacttgtag gtatttatgg cttgttggtt
     5461 aaacgcgacc tggttcgtct tattattgcc tccgggatta cgggctacgc cacaaatttg
     5521 atgctggttt tattcggata caaaagagga gcgatttatc cgattttaga agaaggcaaa
     5581 gccgtcggcg agcggataat ggttgaccct ttgccgcagg cgttggtgtt gacttcgatc
     5641 gtcattgaac ttggtataac ggctatgctg gtagcacttg ccatccgatt attcgagaaa
     5701 aataaaacat ttgatattac taaaatcaag aggttatcag gatgatcctc ccatttttta
     5761 ttataattcc attattgaca gcctttataa ttaccttgat tgccggtgaa cgagacaatt
     5821 gggcggtaat tctctcgatt ctcgccgtgg tttcgctggt gattctttcg gtatattgtt
     5881 tcgtctcgtt gaatgggcgc actctggttt atgaaatggg tcggtggaaa gcgccaatag
     5941 gcattatact ggcggttgat gctctttcgg cctttatgct gatgattgtc agtattatcg
     6001 cgctaacggc gattatgttt tcggtgcagt atattagaaa tttatcgccg tcctggaagt
     6061 attacgcttt atttatgttg ctgacgggtg gaatgaacgg cgttatagcg actggnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttcag cgaagttttt ctagttctgg
     6241 gtaccgtttc gatggttata ggggttttct tggctctggg tcaatgggat gttaaacgtc
     6301 tgcttgccta tcatagcatt agccagatcg ggtacattat tatcggtgtg gggttaggaa
     6361 cgactttagg tctattaggt gctgtttttc atcttttgaa tcacgccata ttcaaagctt
     6421 tgttgttttt gaatgccggt tcgctggaaa tggcattggg tacgcgtgat ttgcgtcaga
     6481 tgggcaaggt ctccaaagct ttgccggtga cttcgtttac ttcgatggtg gcttcgcttt
     6541 cgatttcagg aataccaccg ttcaatggtt tctttagcaa attgataatt ttcatcgccg
     6601 ccatccagat cggccgcccg gtggtggcgt ttttagctgg actcggcagt ttgctgacgt
     6661 tagcttcatt tatgaaagtt caacgttatg gttttcatgg cgagaaaaca gttactgccg
     6721 atcaagctaa agtaggatgg ggtatgcgag cggctatgat tgtgctggcg atactttgtc
     6781 tggcaaccag tgctctggtg attcctggtg tccgggaagt gaccctcgat ccggtggtag
     6841 aagtgattat gaataaaacc ggctacataa cgcgggtgtt agggaggtga aaatgaccat
     6901 caagaatcgt agtcgcgtca ttgtgtttgt gctttcgttt ttggtttggt tggcattgac
     6961 agatataaaa gacatacagg aagtagtcgc aggcttaata gtggcatttc tggttagttt
     7021 aatcgctggg acatttctgg ttacgacgga aaagagccgg catccggttc gacgcttttt
     7081 tatgggaatt gtctatttat tcaaattttt atgggaaatg attaaagcca atattcacgt
     7141 ggcattgatt gttatcaacc ccactttgcc aattaagcct ggcattatca aaatcaagag
     7201 taatttaacc aaagattcgg ccttaactgt attaacgaat tccatcacat tgacaccggg
     7261 tactctgacg gtggacatta atccagaaac tcgtgaaatt tacattcatt gtatcgaggt
     7321 tgaatcaacc gctattgatg aaaatactcg ttccatcggc gggcgttttg aaaagcttat
     7381 cagggaggtt ttcgaatgag agttctgcga tgggtgattg gattggttct aatcggcgcc
     7441 tttatgtatg ctaatttttt catttatacg gcttcgattt atgtcaagtt gataaatgtc
     7501 attcttttca gcgctttgtt tgtgatttac cgagttattg ttggtccgac agccgccgat
     7561 cggattgtag cagtagatat tctgggcata ttgattattg gcatgctggc attactgggc
     7621 ttgaattacg atcaatcttt ttttatggat attggattga tctgggcact tttaagcttt
     7681 attgcctcgc tggcgtttgc caaagtatta gaagggaggt cgctcgatga ttaggacatt
     7741 aatcggtatg gcgttcatcc tgattggggt aacttttgat tttctgggag tattagggct
     7801 ggtgcgtttg ccggatgttt ataatcgtct gcaagcggct accaaatgcg tgacattcgg
     7861 ctcagctggt ttactatttg gagttttcat tctccagggc ttcaatagtt tcggttttaa
     7921 agtgttgata gggattgtat ttattttcct gacttcgcca gtggcggcgc acgtcat
//
LOCUS       DAOMJU010000094         7652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_10096_length_7652_cov_21.522838, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000094 DAOMJU010000000
VERSION     DAOMJU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10096_length_7652_cov_21.522838"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..528)
                     /locus_tag="PLO58_08285"
     CDS             complement(82..528)
                     /locus_tag="PLO58_08285"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQO74827.1"
                     /translation="MERSQFENILDFAIEREWESVRFYHRLLQEAPSLAMMEAIRGIE
                     LMERQHINTLEQFRKAGFKSQAIPSVDNIQLGEYYVPAVENQNYRNMLMTAIQREELS
                     HKLYKALAETSRDPEVADLFEKLASEETQHKSYFESLYDSEVLKEN"
     gene            complement(663..1427)
                     /gene="ccsA"
                     /locus_tag="PLO58_08290"
     CDS             complement(663..1427)
                     /gene="ccsA"
                     /locus_tag="PLO58_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQO74828.1"
                     /translation="MEQPLLIGALILYTIAFVSLITKVKDQICSVVIWLAILTHLAVL
                     IWRSLAAGHPPFTSLYETILLLSFLLALRLVIWRRQIESRYRWIVLLIIMGMIIGALI
                     IPISYKTIQPLMPALNSFWMYLHVPAYFFGYMALVLAVIYALVFLVQNKNESSSDGMT
                     LLRKMDNEVRIAFLFLNIGLVTGAFWGYQSWGNYWSWDVKEVWALLNILVLAFYFHLL
                     KPRGVKKAVIVIIAFLTVIFTYFGVSFLLKGLHSYL"
     gene            complement(1443..2333)
                     /locus_tag="PLO58_08295"
     CDS             complement(1443..2333)
                     /locus_tag="PLO58_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HQO74829.1"
                     /translation="MYKVYLRIFQVSTALLVIALAISTFLSPEAGWGVPLTQPISQIV
                     RALSLDHFPNSWLNVVLWLVLITGMLAAVFLKGIKGFAQKWLHFWLAAVFLLIFYDKA
                     FNQRFVISIQEGQEVNFAQFLKKPKPEYNIPLRLLDFDIKIHPDGKTAASYASRLVVN
                     QTDTAILAVNHPLSIGKYRLYQSAFRKDYLFEVICDGDTIISPFNGACNFNQQEIFLR
                     GVDTLAQALTVEIDRNEYKIPPGEVCNVAGRQIKINPLSPKYTSIIEVAEVTGLKFLA
                     FFSLMYLGVLTYIILWKKIR"
     gene            complement(2465..3292)
                     /gene="mutM"
                     /locus_tag="PLO58_08300"
     CDS             complement(2465..3292)
                     /gene="mutM"
                     /locus_tag="PLO58_08300"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HQO74830.1"
                     /translation="MPELPEVQTIINDLIAANLVGLKILEVEIHWQKIIAEPSAEKFT
                     TEIQNREITKITRRGKFIVIELKPVKFLLVHLRMSGKFSLTVPDYPISKHEHLIFTLN
                     DGRQLRYHDTRKFGRFWLVNQPEKVIGKLGVEPLSADFTVEILTENLRKHHRQIKPFL
                     LDQTIIAGLGNIYVDEALWEACLHPLRMADSLNDQEIASLYQAIPKVLRQGLANQGTS
                     LGNSKSNFQSVGKRGRNQEALKVFRRTGQACPRCGATIERLKVGQRSTHICPHCQLI"
     gene            complement(3305..>4327)
                     /locus_tag="PLO58_08305"
     CDS             complement(3305..>4327)
                     /locus_tag="PLO58_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HQO74831.1"
                     /translation="ELVQYLQRAVKISPEYPTVISKFLENAKEIECDAVARNGEIITY
                     AISEHVENAGVHSGDATLVLPPQRTYLETIRRIRIITSKIAQNLKINGPFNIQFIAKQ
                     NEVKVIECNLRASRSFPFVSKVLKRNFIDIATQVIMHVPLEPIPVSLFELNHVGVKAP
                     QFSFTRLQGADPTLGVEMASTGEVACLGDDFDEAFLKALISVGYHFPPRAALISSGPI
                     ESKAELLESIRTLQNMNVKLYATGGTAKFLEANKVSVETLFWPLEKKEPNVITYLKNG
                     KIDLVINIPKSYQERELTNGYLIRRTAADFNVMLITNRQIAMRFIEAISRLKMDDLKI
                     RSWGEY"
     assembly_gap    4328..4427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4428..6334)
                     /gene="carB"
                     /locus_tag="PLO58_08310"
     CDS             complement(<4428..6334)
                     /gene="carB"
                     /locus_tag="PLO58_08310"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQO74832.1"
                     /translation="MVKKVLILGSAALKIGEAGEFDYSGSQAIKALKEEGIHTILVNP
                     NIATIQTSENLADEIYFLPVNAEFVEKVIKKTRPDGILLSFGGQTALNTGLDLHRRGI
                     LQKYDVAILGTPIQAIKDTEDRQLFIQRLQEIEVKTARSQIATTLDEALQAAEALAFP
                     VIIRIAYALGGLGSGVCRNLEEFRDKAEKALTYAPQILIEEYLEGWKEIEYEVICDSF
                     NNCLTICNMENVDPMGIHTGESIVVAPCQTLSNSESHKLRSIAIKVVRHLGIVGECNI
                     QFALDPRSEDYRVIEVNARLSRSSALASKATGYPIAFIAAKLALGYGLHQLPNKVTRT
                     TTAFFEPALDYIVVKIPRWDLKKFRNVSHRIGSGMKSVGEVMAIGRTFEEALQKGLRM
                     LETGANGIVGNDGVIFENLERELSEPTDERIFAVAEALQQGYDVAKIAKLTNINPWFI
                     YKIRNIVAMNTHLSQYRNQELPTDTLLAAKRLGFSDKQIALAIGKSDAEVHQLRLKAN
                     IRPFIRQIDTLAAEFPAYTNYLYLTYNAIRDDISFTDSNVVMILGSGAYRIGSSVEFD
                     WSCVNCGQTLRRLGYNTIMLNYNPETVSTDYDESDFLIFDEISFENVVEIYAKFSALG
                     VIVSMGGQIPN"
     gene            complement(6483..7535)
                     /gene="carA"
                     /locus_tag="PLO58_08315"
     CDS             complement(6483..7535)
                     /gene="carA"
                     /locus_tag="PLO58_08315"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HQO74833.1"
                     /translation="MNKRTALLTLEDGHVFEGFSFGWEGATAGELVFNTGMVGYPESL
                     TDPSYYGQILVMTYPLIGNYGVPEFKPDSLHFESDRIQVRGLIVADYSADYCHWNAHQ
                     SLGDWLKSQKIPAITGIDTRTLTQILREEGTMLAKITFDDDIDYYDPNRYNVVAEVTC
                     SEPRTYGQGKKRIALLDCGCKRSIISQLSQHKVEIMRLPWDSDLTKYDFNGLCISNGP
                     GDPRNCEASVASVRLALENKIPTLGICLGNQILALAIGAKTYKLKYGHRGQNQPVMDQ
                     TSGCCYITSQNHGFAVDDKSLPEDWRIWFSNLNDRTNEGLIHRSGRYMSVQFHPEAAT
                     GPLDTMFVFDRFLDIL"
BASE COUNT         2154 a         1668 c         1572 g         2158 t
ORIGIN      
        1 cgcaacgttt aatgaatttt cactgaaaat ttccgtaagt taaaatcaag acaaatcttg
       61 attctctcca attcacccgg tttaattttc cttgagcact tcggaatcgt ataggctctc
      121 aaaataactt ttatgctgtg tctcttccga agctaatttt tcgaacaaat ccgctacctc
      181 gggatcacga ctggtttcgg ctaaagcttt atataattta tgagaaagtt cttcgcgctg
      241 aatagcggtc ataagcatgt tgcgataatt ttgattttcg actgcaggta cataatattc
      301 accaagttga atattatcaa ccgatggaat tgcctgcgat ttgaaaccgg ctttacgaaa
      361 ttgttctaat gtattgatat gctgacgctc catcagctcg attcctcgaa tagcttccat
      421 catagccagt gatggcgctt cctgcaacaa acggtgatag aaacgaacag attcccattc
      481 gcgctcaatt gcaaaatcca gtatattttc aaattgactg cgctccatcg tttcccccgc
      541 gatttattaa ttcaataaac tatatgcaat ttagcctaat ttattctaca acgcaagatt
      601 tctgccgctc ccggatttca aaccacatta ctatatcgtc ttgaatagaa aaatagtatt
      661 tatcaaagat aactatgcag tccttttaat aaaaaactca ctccgaaata agtaaaaatc
      721 actgtcagaa aagcaattat tacgattacc gctttcttga ctcctctcgg ctttaacaag
      781 tgaaaataaa atgctaaaac tagaatatta agcaatgccc agacttcttt gacatcccac
      841 gaccaataat tgccccagct ctgatagccc cagaaagctc ccgtcactaa tccgatattc
      901 agaaagagaa aagcaattcg cacttcatta tccatttttc tcagtaacgt cattccatcg
      961 cttgaacttt catttttatt ttgcaccaga aaaaccaaag cataaataac tgctagaacc
     1021 agcgccatat aaccaaaaaa atatgccggc acgtgcagat acatccagaa actattcaag
     1081 gcaggcatta acggctgtat cgttttatat gaaatgggaa ttattaaagc gcctataatc
     1141 attcccataa ttatcaataa cactatccag cgataccgag actcaatctg ccgccgccag
     1201 atgactaacc tcaaagcaag taaaaaagat aatagaagaa tagtttcata gagcgaagtg
     1261 aatggcggat gcccagccgc caacgatcgc caaatcagca cggctaaatg ggttaaaatg
     1321 gctaaccaga tcacaacgga acaaatttgg tctttgactt ttgtaatcag actaacaaaa
     1381 gcaattgtgt ataaaatcaa ggcgccgatt aaaagcggtt gttccactgc ggtttcccct
     1441 ttttaacgaa ttttcttcca taaaatgata taggtcagca cacctaaata cattagacta
     1501 aaaaatgcga ggaactttaa gcctgttact tcggcgactt caatgattga ggtgtacttt
     1561 ggacttagcg gattaatttt aatttggcga ccggcgacat tgcatacctc accgggtgga
     1621 attttatatt cgtttcgatc aatttcgaca gtcaacgcct gcgctaaagt atcaacacct
     1681 ctcagaaaaa tttcttgctg attaaagttg caagccccat taaaaggcga aattatggta
     1741 tctccgtcgc aaattacttc aaataaataa tcctttctaa aagcactttg atataaccga
     1801 tatttaccga tactgagcgg atggttaaca gccagaatgg ctgtatcggt ttgattaacc
     1861 accagtcggc tggcgtaaga cgccgccgtt tttccatcag gatggatttt tatatcgaaa
     1921 tcgagaagtc gcaatggaat attgtattct ggttttggtt ttttcaaaaa ttgggcaaaa
     1981 ttgacttcct gcccctcctg aatcgagata acaaaacgtt ggttgaaagc tttatcataa
     2041 aagattagga gaaaaacagc cgccagccaa aaatgcagcc atttttgagc gaaacccttt
     2101 atgcctttta gaaatacagc cgctaacatt ccagttatca acaccagcca gagaacgaca
     2161 tttaaccagg aatttggaaa gtggtcaagg ctaagcgctc gcacaatttg cgatattggt
     2221 tgagtcagag gaacgcccca tccggcttcg ggagacagaa aagtacttat ggccaaagct
     2281 atgaccagca acgccgtcga aacctgaaat attcttaggt aaactttata caagacaatt
     2341 actgtataat aaaatctgcc aaattgggaa atcgtttaaa gctatcttta ttcacgtatt
     2401 aaaattaaag gaaattttca gggaatcaaa aagacggaca ttcagataaa ataattgaga
     2461 taggtcagat tagctggcaa tgcggacaga tgtgtgtact tctctggcca actttgagac
     2521 gttcaatagt cgcaccgcaa cgcgggcaag cctgaccggt acggcgaaag actttcagcg
     2581 cttcttgatt acgaccacgc ttgccaaccg attgaaaatt gcttttgcta ttcccgaggc
     2641 ttgtcccttg attggctaaa ccctgtcgta ggactttggg aatagcttga tacaaagatg
     2701 caatttcctg gtcattgaga gaatcagcca ttcttaaagg atgaaggcag gcttcccaga
     2761 gcgcttcgtc aacgtaaata ttgcccaaac cagctataat tgtctgatcc aataaaaacg
     2821 gtttaatctg acggtgatgt ttacgcaaat tttcagttag aatttcaacc gtgaaatcgg
     2881 cacttagcgg ctcaacgccc agtttaccaa ttactttttc gggctgatta actaaccaga
     2941 aacgcccgaa cttgcgagtg tcatgataac gtagttggcg accatcattt agcgtgaaaa
     3001 ttaaatgttc atgtttgcta attggataat cgggcactgt cagggagaat ttccctgaca
     3061 tacgcagatg aacgaggaga aatttgaccg gctttagttc aattacaata aattttccgc
     3121 gtcgggtaat tttagttatt tctcggtttt gaatctcggt tgtgaacttt tcggcgctag
     3181 gttcggcgat gattttttgc cagtgaatct caacctcaag aattttcaga ccgactaaat
     3241 ttgcggctat aagatcattg atgatagttt ggacttccgg taattcgggc acggagcggt
     3301 ttcctcaata ttcgccccaa cttctgattt tcaaatcatc cattttcaag cgagagatag
     3361 cctcgatgaa gcgcatcgcg atctggcgat tggtaattaa cattacatta aaatcagccg
     3421 ctgttcgccg aattaaatag ccattagtca attcgcgttc ctggtagctt ttcggaatgt
     3481 tgatcaccaa atcaattttg ccatttttga gataggtaat gacgttcggt tcttttttct
     3541 cgagcggcca gaacaaggtt tcaaccgaaa ctttatttgc ttcgaggaat tttgccgttc
     3601 caccggtggc gtacagcttc acgttcatat tttggagggt tcggattgat tccagtagtt
     3661 cggctttaga ttcgattggg ccggatgaaa ttaaagccgc gcgcggcgga aaatggtagc
     3721 cgaccgaaat cagcgctttc agaaaagctt catcgaaatc gtcaccgagg caagcgactt
     3781 cgccggtaga agccatttct acgccgagag tcggatcagc accttgcaaa cgtgtaaagg
     3841 aaaattgggg tgccttgaca ccgacgtggt ttagttcgaa cagtgatact ggtattggtt
     3901 cgagtggcac gtgcataata acctgggtag cgatgtcaat aaaattgcgc ttcaagactt
     3961 ttgaaacaaa cggaaaactg cgcgatgccc gtaaattaca ttcgattact tttacctcgt
     4021 tctgtttggc aatgaactga atgttgaacg gaccattaat ttttaaattc tgggcgatct
     4081 tgctggtaat aattcgaatg cgtcggatag tttccagata agtgcgttgc ggcggcagga
     4141 ccaaagtcgc atcgccggaa tgaacgccgg cgttctcgac atgttccgaa atagcgtagg
     4201 tgataatttc gccattacgg gcgacggcgt cacattcaat ttccttagca ttttccagaa
     4261 atttgctaat gacggtcgga tattccggcg aaatttttac ggcgcgttgt aaatattgga
     4321 caagttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta ttgggaatct
     4441 gaccacccat cgaaacgatt actccgaggg cgctgaattt ggcataaatt tccactacat
     4501 tttcaaaact aatttcatca aaaatcagga aatcggattc gtcataatcg gtgctgaccg
     4561 tttctggatt gtaattcagc ataatggtat tatatccgag tctccgcaga gtttggccgc
     4621 aattcacgca actccaatca aattcgaccg aactaccgat acggtaagcc cctgaaccaa
     4681 gaatcatgac cacattggaa tcggtaaaac taatatcatc gcgaatggca ttataggtta
     4741 ggtaaaggta attggtataa gccgggaatt cagccgctag cgtgtcaatc tgtcgaataa
     4801 aaggtcggat attggctttc agacgcaatt ggtgcacttc ggcatctgat ttcccgatag
     4861 cgagcgcgat ttgcttgtcg gagaagccaa gtcttttggc ggctagtaag gtatcggtcg
     4921 gtaattcttg atttcgatac tggctcaagt gtgtattcat tgcgacgata ttacggattt
     4981 tgtaaataaa ccagggattt atgttggtta acttggcgat tttagcgacg tcatagcctt
     5041 gctggagagc ttccgcgact gcaaagatac gttcatcggt cggttcgctc agttctcgtt
     5101 ccagattttc aaatattaca ccgtcattgc cgacaatacc attggcaccg gtttcgagca
     5161 tacgcaagcc tttttggaga gcttcctcga atgttcgtcc aatcgccatt acttcgccga
     5221 ctgatttcat ccctgaaccg atgcgatgac tgacgttgcg gaattttttc aaatcccagc
     5281 ggggaatttt aaccacgata taatccagag ccggttcgaa gaaggcagtc gtcgtacgtg
     5341 ttactttatt aggcaattga tgcagtccgt agccgagtgc tagttttgcg gcaatgaagg
     5401 caatcggata gccggttgct ttggaagcca gcgccgaact gcgcgacaat cgggcattca
     5461 cctcgatgac gcgataatcc tctgaccgcg gatcgagtgc aaactggatg ttacattcac
     5521 cgactatccc gaggtgccga acgactttta tagctataga acgcaactta tggctttcgc
     5581 tattggaaag tgtttggcag ggcgctacca cgatgctttc gccggtatga atgcccatcg
     5641 gatccacatt ttccatattg cagatcgtca ggcagttgtt aaaactatcg caaattactt
     5701 cgtactcgat ctctttccag ccttccagat attcctcgat caaaatctgg ggggcataag
     5761 tcagtgcttt ttcggcttta tcacggaact cttccagatt acggcagacg cctgaaccca
     5821 gtccgccgag agcatacgct atccgaataa taaccgggaa agctagagct tcagcggctt
     5881 gtaaagcttc atccagagta gtggctatct gactacgtgc agttttgacc tcaatttcct
     5941 gcagacgttg gataaaaagt tggcggtctt cagtatcttt aatggcttga ataggagtcc
     6001 ccagaattgc aacatcatat ttttggagaa taccgcgacg gtgcaagtcc agaccagtat
     6061 taagggcggt ctgtccgcca aagctaagca aaataccatc aggtctggtt tttttaatta
     6121 ctttttcgac aaattcggcg ttaaccggta ggaagtagat ttcatcggct aaattttccg
     6181 aggtctgaat cgtagcaata ttgggattta ccagaattgt atgaatgcct tcttctttta
     6241 aggctttgat ggcttgactg ccagaataat caaattcccc ggcttctccg attttaaggg
     6301 cggcactgcc gaggatgaga acttttttaa ccattttttc ctttcaatgc tgagggcgct
     6361 gagatcgcca agactgataa ttcaatccaa cttttttccc ggttatttgt tttgagaatt
     6421 tttataattg gcattataat atccattaat taacactgaa ttgtttttcg cgttctctcc
     6481 tcttataaaa tatccaggaa tcggtcgaat acaaacatcg tatcaagcgg gccagtcgcg
     6541 gcttcaggat gaaactgaac gctcatatac cgcccggagc ggtggattaa gccttcatta
     6601 gttcgatcat tcaaattgct aaaccagatt cgccaatcct caggcaagga cttatcatca
     6661 accgcaaagc catgattctg cgaggtgatg taacagcatc cgctggtttg atccattacc
     6721 ggttgatttt gtccccgatg gccatattta agtttataag tctttgcccc tatcgccaaa
     6781 gctagaattt gattaccgag gcaaatgccc agagtcggta tcttattctc taatgccagt
     6841 cgaactgagg caacagaagc ttcacaattt cgaggatcac ccgggccatt agaaatacac
     6901 aacccgttaa aatcatactt ggtcaaatcg ctgtcccaag gcaaacgcat aatttcaact
     6961 ttgtgctgag acaattgcga gattatactc cgcttacatc cacaatccaa aagtgcgatt
     7021 cgtttcttgc cctggccata cgtccgcggt tcggagcagg ttacttccgc taccacgttg
     7081 taacggttag gatcatagta atcaatgtcg tcgtcaaaag taattttggc gagcattgtg
     7141 ccttcttccc gtaaaatctg agttagagtg cgggtatcaa tgccggttat ggctggaatt
     7201 ttttgtgatt ttagccaatc gcccagcgac tggtgggcat tccaatgaca ataatccgct
     7261 gaataatcag ctactatcag cccacgaacc tgaatccgat cggattcaaa atgcaggcta
     7321 tcgggcttga actctggcac accgtaatta ccgattaatg ggtaagtcat cactaaaatc
     7381 tgtccgtaat aggaaggatc agtcagactc tctgggtacc cgaccatacc ggtattaaag
     7441 actagttcac cggcggtcgc accttcccag ccaaaagaaa aaccctcgaa aacatgaccg
     7501 tcctcgaggg ttaaaagcgc cgtgcgttta ttcattgatt tacttgactt tccaagaatt
     7561 ttattcagtt aatttcatag atcttcaatc ggggcgtaat ttaatcaatc aattcaagaa
     7621 tttcagtaaa tttttctggc ttaaacttga ct
//
LOCUS       DAOMJU010000095         7468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_10436_length_7468_cov_20.632403, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000095 DAOMJU010000000
VERSION     DAOMJU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10436_length_7468_cov_20.632403"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLO58_08320"
     CDS             <1..660
                     /locus_tag="PLO58_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQO74834.1"
                     /translation="QLIPPLKKMRIPIIGLLGRNESTLAKKCDYVISTQINGEADPLQ
                     LVPTTSAIATLAMGDALAISLLAKRHFNRRDFAGLHPSGSLGRRLLTTVADLMHTGEE
                     IPFVKSSDKMPEVLYMMTKKSLGIVGVLDDSENLIGVVTDGDLRRGLERQKDFMEQTA
                     IKVMSHNPKWIKKSSLAIEALHLMEEHSITNLFVYNGEQNRKPIGIIHIHDILRYGIM
                     P"
     gene            657..1196
                     /gene="lptC"
                     /locus_tag="PLO58_08325"
     CDS             657..1196
                     /gene="lptC"
                     /locus_tag="PLO58_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQO74835.1"
                     /translation="MKGRFLISTLFVVLFCWGCTEDKSYEPTMIDRQYPDQEIWNSNI
                     TLTKDGQKRAVVHSGHLTKNNDQALIYMDTDVAVDFYDTEGHHLSHLTSQTATVNEHT
                     NDLIAKGNVVVVSDSGFTLFTEELRWDHRRERIISEVFVTFVTPEDSLTGIGFESESD
                     LKNWVIFKPSGVTSRELKY"
     gene            1200..1952
                     /gene="lptB"
                     /locus_tag="PLO58_08330"
     CDS             1200..1952
                     /gene="lptB"
                     /locus_tag="PLO58_08330"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQO74836.1"
                     /translation="MELNSQPQKKTLRGENLVKIYGKRRVVDSVNIEVSKGEVVGLLG
                     PNGAGKTTTFHMFTGMIKPTSGKVFINEQEITRLPMYKRAQLGIGYLSQEPSVFRKLT
                     VEQNLMLVLQTLGISRKEQKERLEILLDQLELRKLRKRRADTLSGGERRRTEITRALI
                     TNPDFILLDEPFVGVDPIAVEDIQKIVVSLKDRDIGVLITDHNVHETLSITNRSYLMF
                     EGRILKSGTSEFLANDEDARRLYLGEAFKLAR"
     gene            1952..3340
                     /gene="rpoN"
                     /locus_tag="PLO58_08335"
     CDS             1952..3340
                     /gene="rpoN"
                     /locus_tag="PLO58_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQO74837.1"
                     /translation="MQLSQSQVQKLSQELRLTPQQILQSTILQLNTLALEARVLQELE
                     QNPVLEEVNPEVSESSEEELQDNQETSQEDDIDWEEILPQNDDYTPKEERDSDQKKTQ
                     FVQPAPRGLVEHIFDQLKLHNLDDEEERIAAEIVWNLDENGYLTLPIENIAFGLGLPQ
                     KKVLKVLKEIQELDPPGLAARDLKECLLIQLNQIPNSDDAIKVISEHFEDFANHRYQN
                     IMKAYGWDKERMQAVQEMITKLNPKPGATLNTLDPQYIVPDLIVEKVDGEYIVEVNDT
                     RIPELQISSTYLQMISNGSKIDNETKNYLKRKIDNAKWFIQAIQQRRLTMIRVMKAII
                     ARQLDFFEDPSKPLKPMILKDIADDVNMDISTISRVTNNKYVQLPSGIYELKYFFNEG
                     IKDEDGEEISTRIVKEKLKEIIDNEDKHKPYSDEQLSNLLKQNGYLVARRTVAKYREQ
                     MKIPVARLRRVV"
     gene            3372..4082
                     /locus_tag="PLO58_08340"
     CDS             3372..4082
                     /locus_tag="PLO58_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HQO74838.1"
                     /translation="MAGKKVQFSNKEIEVSSVSYWLIILIALVLVVIVTALMSFYTIE
                     PGETGVVTRCGKVHQINGPGAHWKLPWGIDRLYRVKVNYPYTAEFGIRTTSDSLTALS
                     DNFYLPEARLLTGDLKMVDLQWVVQYSIQDPVAYLFGVENPENMLRLVGAAAMRSAVG
                     DFSFREVFQTERRAVAERAKNYMQEKFDLYKTGIVIQHVQLKEARPALPLSEPDTTIS
                     LKPFNQTDSTEADSVKKI"
     gene            4160..4780
                     /locus_tag="PLO58_08345"
     CDS             4160..4780
                     /locus_tag="PLO58_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQO74839.1"
                     /translation="MRFKSFIWLLILALIIIGAERATVILEEDEQMIVARFTQPTVTP
                     VTTAGVHLIIPFLAKTYIYPNNLIEWQSNVIEIPTKDDKFIAIESFAYWKISDPLLFY
                     ERVRTIPEAENRLTDLLDGSLRDEVARYTLAELIRSSNRRMDIAEVKDILKVPQSRTL
                     FNVKGRRPAIIAGILKNATATLDSLQLGIQLVDFDLKSVNFVRIIE"
     gene            4793..5341
                     /gene="hslV"
                     /locus_tag="PLO58_08350"
     CDS             4793..5341
                     /gene="hslV"
                     /locus_tag="PLO58_08350"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HQO74840.1"
                     /translation="MQEKPIPRIIATTVIGVRRNGQAAMGSDGQVTIGETIMKHTTKK
                     VRRIFNDQVVVGFAGASADAMALFDKFENELEKYKGNVNRAAVELAKIWRTDKYLRQL
                     EALLTVMNKETMLIISGTGDVIEPDDDIIAIGSGGAFATAAARALLRHTEMNAREIVA
                     ESLRIASEICIYTNNNFTIIEL"
     gene            5338..6702
                     /gene="hslU"
                     /locus_tag="PLO58_08355"
     CDS             5338..6702
                     /gene="hslU"
                     /locus_tag="PLO58_08355"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQO74841.1"
                     /translation="MKNRELTPREIVRELDAYIIGQEKAKKAVAIAMRNRWRRQHVRG
                     AIKEDILPNNIILIGPTGIGKTEIARRLSNLAGAPFIKVEASKFTEVGYVGRDVESII
                     RDLTGIAVTMVKNERMAEVNSQALFLANERILDILLPSGETKHHSEQEEIIPRSDEKF
                     NRTREKLRVMLENGEMEDRLIEITVNQGAMPMMQIFGPAGIEEMEMNLSEMLGSAFPQ
                     KKKIQKVTISEARKIFTQEEAQKLIDMETVVREAVQRVEETGIVFIDEIDKIASSGDQ
                     RGYGPDVSREGVQRDILPIVEGSNVQTKYGVVRTDHILFIAAGAFHATKPSDLIPELQ
                     GRFPIRVEMDSLGKDEFVKILKNPRNALLKQYTALLKTEKVELIFDDDAVEEIAQIAA
                     EVNEKMENIGARRLHTILTTLLEEILYKVPDKDIREVHITREFVQARLQNIVRDRDLS
                     RYIL"
     gene            6728..>7468
                     /gene="argF"
                     /locus_tag="PLO58_08360"
     CDS             6728..>7468
                     /gene="argF"
                     /locus_tag="PLO58_08360"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQO74842.1"
                     /translation="MKRDFLHITDFSTAEIWEVFELTKKLKSYLKEGWEYTPFKGKTM
                     AMIFAKPSARTRISFETGMTQLGGHAIYLGPNDIGLGQREAVKDIAQVVARYDDIIMA
                     RLFAHAHILELAEFSSVPVINGLTDYNHPCQVMADIFTVLEKRGNLDNLKVTFVGDGN
                     NVVNSWLELTLRLPMHFVLACPPGYEPDKELLTKAQNSGLSKVEISHEPFEAVQNADV
                     IYTDVWASMGRETEKEKRKCDFAGFQVNL"
BASE COUNT         2248 a         1546 c         1740 g         1934 t
ORIGIN      
        1 caattaattc caccgctgaa aaaaatgcgc attcccatca ttggcttatt ggggcgtaat
       61 gaatcaactc tggcaaaaaa atgtgactat gtcattagta ctcaaataaa tggtgaagca
      121 gatccattac aattggtacc gactaccagt gcgattgcaa ccttagctat gggtgatgcg
      181 ctggcgatta gtttgctcgc gaaacgtcat tttaatcggc gcgatttcgc cggtttacat
      241 cccagcggtt cactgggacg cagactccta actacggtag ccgatttgat gcacaccggt
      301 gaagaaatac cgtttgtcaa gtccagcgac aaaatgcctg aggtgcttta tatgatgacg
      361 aaaaaaagcc tcggcattgt cggcgtcctg gacgattccg aaaacctgat tggtgttgtc
      421 actgacggtg acttgcgccg cggtctcgaa aggcagaagg attttatgga acaaactgcc
      481 ataaaagtta tgtcccataa tccgaaatgg attaaaaaaa gtagtttagc gattgaagcc
      541 ttgcatctaa tggaagaaca ttcgatcacc aaccttttcg tatataatgg cgagcaaaat
      601 cgcaaaccga tcggaattat tcatattcat gatattttgc gctacgggat tatgccttga
      661 agggaagatt tttaatatca accttatttg ttgtcctatt ttgctggggt tgcacagaag
      721 ataagagtta tgagcctacc atgattgacc ggcaataccc ggatcaagaa atctggaatt
      781 cgaatattac cctgactaaa gacggccaaa aacgagccgt tgttcattct ggccatttga
      841 cgaagaataa tgatcaggcg ttgatatata tggataccga cgtggcagta gatttctatg
      901 atacggaggg acatcattta tcgcacttga cttctcagac agcgactgtt aatgaacaca
      961 cgaacgatct aattgccaaa ggtaatgttg tagtcgtttc tgatagtggc tttacccttt
     1021 tcaccgagga attacgctgg gatcaccgaa gagagcgaat tatttcagaa gtttttgtaa
     1081 cctttgtgac tcccgaagat tcgctaaccg gaattggatt tgaatccgaa tcggatttga
     1141 aaaattgggt gattttcaaa ccttctggag taaccagccg ggagttgaaa tactaaaata
     1201 tggaattaaa tagccaaccg caaaaaaaaa ctctgcgagg tgaaaaccta gtaaaaatat
     1261 acggcaaacg ccgggtcgtt gattccgtca atatcgaagt gtccaagggc gaagtggtcg
     1321 gcttgctcgg accgaacggt gccgggaaaa cgactacatt tcatatgttt accggtatga
     1381 tcaagccgac ttcgggaaaa gtattcatta atgaacagga aatcacgcgt ttgccgatgt
     1441 acaaacgcgc ccaactagga attggttatc tctcacaaga gccatcggtt ttccgtaaat
     1501 taaccgtcga gcaaaatttg atgctggtac ttcaaacact gggcattagc cgaaaggagc
     1561 agaaggaacg actcgaaatc ctgctcgacc agctcgagct tcgcaagtta cgcaaaagac
     1621 gggcggacac cctctccggt ggcgaacgac gccgcacgga aattacccgc gccttgatta
     1681 ccaacccgga ttttatacta ctggacgaac catttgtcgg cgttgatccg attgcagtgg
     1741 aagatattca aaaaatcgtt gtcagcttaa aagatcggga cattggcgta ttgataactg
     1801 atcacaacgt gcacgagacg ctttcgatta ctaaccgttc ctacttaatg tttgaaggtc
     1861 gtattcttaa atcggggaca tctgaatttt tagctaacga tgaagatgcc cgccgcttat
     1921 atttaggaga agcattcaag ctcgccagat gatgcagtta agtcaatccc aggttcaaaa
     1981 actttctcag gaattgcggc tcacgcccca gcagattcta caatccacga ttttgcaatt
     2041 gaatacctta gctctcgaag cccgcgtact tcaggaactt gaacagaatc cggttttgga
     2101 agaagtaaat cctgaggtta gtgaaagttc cgaagaagaa ttgcaggata atcaggaaac
     2161 ttcccaagaa gatgacattg attgggagga aattcttccg caaaacgacg attatacacc
     2221 caaagaagaa agagattccg accagaaaaa aacgcaattt gttcagccag caccgagagg
     2281 attggttgaa catatattcg atcagttgaa attacataat cttgatgatg aagaagaacg
     2341 gattgcggct gaaatcgtct ggaatcttga tgagaatggt tacctcaccc tgccgataga
     2401 aaatatcgcg ttcggactgg gattgcccca gaaaaaagtg ctgaaagtgc tgaaagaaat
     2461 ccaggaactc gatccgcccg gtttagccgc tcgcgatttg aaagagtgcc ttctgatcca
     2521 gcttaatcaa ataccgaatt cagatgatgc catcaaagtc attagtgagc atttcgagga
     2581 ttttgccaat catcgctatc aaaatattat gaaagcctac ggctgggaca aggaacgaat
     2641 gcaagccgtt caggagatga taaccaaact caatcccaaa ccgggtgcga ctttgaacac
     2701 gctcgatccg caatatatcg tccctgattt gatcgtcgaa aaagtagatg gagagtatat
     2761 tgtcgaagtc aatgatacgc gcatcccgga attgcaaatt agttccacct atttgcagat
     2821 gattagcaat ggttctaaaa ttgacaatga gaccaagaat tatttgaagc gcaaaattga
     2881 taacgccaag tggtttattc aagcaattca gcagagacgc ctgactatga ttcgggttat
     2941 gaaagccatc attgcccgac aattggattt cttcgaagat cccagtaaac cgctgaagcc
     3001 gatgattcta aaagatattg ccgatgatgt caatatggat atctcaacta tcagccgagt
     3061 cactaataat aaatatgtcc agttgccctc aggcatttat gagttaaaat actttttcaa
     3121 cgaaggtatt aaagacgaag acggtgagga aatttccact cggatagtca aggaaaagct
     3181 gaaggaaatt attgataatg aagacaaaca taaaccttat agtgatgagc agttatccaa
     3241 tttactcaag caaaacggtt atttagttgc ccgtcgcaca gtcgctaaat accgtgagca
     3301 aatgaaaatt ccggttgctc gcttgcgacg cgtggtttga tgtttttctt atgaaattat
     3361 caggaggaaa aatggctggt aaaaaagttc agttcagtaa taaagaaatc gaggtttcga
     3421 gcgtttccta ctggttgata attctgattg cgttagtgtt ggtagtaatt gttacggctt
     3481 tgatgtcatt ttatacaatt gagcccggcg aaaccggtgt agtaactcgc tgcgggaaag
     3541 ttcaccagat caatggacct ggtgctcatt ggaaattacc ttggggcatt gatcgcctat
     3601 atcgggtaaa agttaattac ccttacactg ccgaatttgg tattcggact acctccgata
     3661 gtctgactgc tctgtcggat aatttctatc taccggaagc gcgtttgcta accggtgact
     3721 tgaaaatggt tgatttgcaa tgggtggttc agtacagcat tcaagacccg gtagcctatt
     3781 tatttggagt tgaaaatccg gagaatatgt tacgattagt cggcgcggcg gctatgcgtt
     3841 cggcagttgg tgacttttcc tttcgcgaag tattccagac tgagcgacga gcagtcgccg
     3901 agcgagctaa aaattatatg caagaaaaat ttgatcttta taaaactgga attgtcattc
     3961 agcacgtgca gttgaaagaa gcgcgaccgg ctttaccgct atccgaaccg gatacaacta
     4021 tatcactaaa gccgtttaat caaaccgatt caactgaagc cgattcagtc aaaaaaattt
     4081 gatgaaaact ggtaggagtg aaataaaaca gccaaaatgc gcctgatttc tacaagaaat
     4141 tatctcacgg aggtctcgga tgaggtttaa gtcatttatc tggttattga ttctagcgct
     4201 gattatcatt ggggcggaga gagcgacggt aattttagaa gaagacgagc aaatgatcgt
     4261 ggctcgcttt actcaaccga ctgtaacgcc ggtaacaaca gccggtgttc atctaataat
     4321 accgttttta gcgaaaacct atatttaccc caacaacttg atcgaatggc agagcaacgt
     4381 aattgagata cctactaaag atgacaaatt catcgccatc gagtcctttg cctattggaa
     4441 aatatccgat ccgctgttgt tttatgaaag agtcagaaca attccggaag ccgaaaatcg
     4501 cctaactgat ttattggatg gttctctgcg ggacgaagtc gcccgctata ctcttgcgga
     4561 gcttattcga agcagtaacc gtcgaatgga cattgccgag gttaaagaca ttctgaaagt
     4621 ccctcaatcc aggacattgt tcaatgtgaa aggtcggcga ccagccatca tcgccgggat
     4681 tttaaaaaat gcaactgcta cgctggattc tctgcaattg ggtattcagc tggtagattt
     4741 tgatctaaaa agtgtaaatt ttgttcggat aattgagtaa aatggagata atatgcagga
     4801 aaaaccaata cccagaataa tagcaactac cgttattgga gtgcgtcgta atggccaagc
     4861 cgctatggga agcgacggtc aagtgacgat cggcgagacg attatgaaac atacgaccaa
     4921 gaaagtccgt cgaattttca atgatcaggt tgtggtcggt tttgccggag catcggcgga
     4981 cgctatggcc cttttcgata aattcgaaaa tgaattagaa aaatataaag gtaatgtaaa
     5041 ccgtgcggcc gtcgagttgg ctaaaatctg gcgcaccgat aaatatctac ggcagttaga
     5101 agctctttta accgtaatga acaaggaaac catgttaatt atttccggaa cgggtgatgt
     5161 gattgagccc gacgatgata ttattgcgat aggttcgggt ggcgcctttg ccaccgccgc
     5221 cgcccgcgcc cttttacgcc ataccgagat gaatgcccgg gaaatcgtgg ctgaatcact
     5281 ccgaatcgct tccgagattt gtatttacac caacaataat tttacaatta tagaattatg
     5341 aaaaatagag aacttacacc gcgcgaaatc gttcgcgaac tcgatgccta cataatcggt
     5401 caggaaaaag ccaaaaaagc tgttgccatc gccatgcgaa atcgctggcg gcgccaacac
     5461 gtgaggggcg ccataaagga ggatatttta ccaaacaata ttatcctgat tggtccgacc
     5521 ggcatcggca agactgaaat cgctcgtcga ctttctaatt tagccggagc acctttcatc
     5581 aaagtcgagg catccaaatt taccgaagtc ggttacgtcg gacgagatgt cgagtcaatc
     5641 attcgtgatc taaccggtat cgccgtgact atggtaaaga atgaacgtat ggcggaagtc
     5701 aatagtcaag ctctttttct ggctaacgaa cggattctcg atattctact tccttcaggg
     5761 gagacaaagc accattcgga gcaagaagaa attatcccta gaagtgatga aaaattcaat
     5821 cggacacgtg aaaaattgcg cgtgatgctt gagaatggtg aaatggaaga ccgcctgatc
     5881 gagatcacgg tgaatcaggg agcgatgccg atgatgcaga tttttggtcc agccggtatc
     5941 gaggagatgg agatgaatct ctccgaaatg cttggtagtg ctttcccgca gaagaaaaaa
     6001 atccagaaag taaccatttc cgaagcccgt aaaattttta cccaggaaga agcacaaaaa
     6061 ttaattgata tggaaacggt agttcgggaa gcggttcagc gagtcgaaga aaccggaatc
     6121 gttttcattg atgaaattga taaaatcgca tccagcggcg atcagcgtgg ctacggtccg
     6181 gatgtttcgc gcgaaggtgt ccagcgcgat attctaccca tcgtcgaagg cagtaatgtc
     6241 cagaccaaat acggagtggt tcgcaccgat catatacttt ttatcgcggc tggggctttc
     6301 cacgctacca aaccttctga tttgattcca gaattacagg ggcgctttcc catccgagtt
     6361 gaaatggata gcctcggcaa agatgaattc gtcaaaattt tgaaaaatcc gcgtaacgct
     6421 ttgctcaaac aatataccgc tctactgaaa actgagaagg ttgagttaat tttcgacgac
     6481 gatgcagtgg aggaaattgc ccaaattgcg gcggaagtca atgagaaaat ggaaaatatc
     6541 ggcgcacgcc gtctgcatac gattctgaca accttactgg aagaaatcct ctataaggtt
     6601 cctgataaag atattcggga agtacacatt acgcgcgagt ttgtccaagc aagattacaa
     6661 aatatagtgc gtgaccgcga tttgagtcgt tatatacttt aatttattga ttcacctgag
     6721 ggatttaatg aaaagggatt ttttacatat tactgatttt tcgacggcag aaatatggga
     6781 agtgtttgaa ctgacgaaaa agctcaaaag ctacctcaaa gaaggttggg agtacactcc
     6841 gttcaagggc aaaactatgg cgatgatttt tgccaaaccg agcgctcgca cgcgcatttc
     6901 tttcgagact ggtatgactc agctaggtgg gcacgccatt tatttagggc cgaatgacat
     6961 cggccttggc caacgggaag ccgtcaaaga tattgcccaa gttgtggctc gctatgatga
     7021 tataattatg gcgcgacttt ttgcgcatgc ccatattctc gaattagccg aattttcatc
     7081 tgtaccggtt atcaatggct tgaccgatta caatcacccc tgtcaggtaa tggctgatat
     7141 ttttactgtg ctggaaaaac gtggcaatct tgataatctc aaagtcactt tcgtcggcga
     7201 cggtaataat gttgtcaatt cctggctgga actgactttg agacttccca tgcattttgt
     7261 tctggcttgc cctcccggtt acgaaccgga caaggaattg ctgaccaaag ctcaaaatag
     7321 cggactaagc aaggtagaaa tatctcacga gccatttgaa gcggtccaaa atgccgatgt
     7381 gatatatact gatgtctggg caagtatggg acgcgaaacc gaaaaggaaa agcgtaagtg
     7441 cgactttgcc ggattccagg taaatctg
//
LOCUS       DAOMJU010000096         7161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_11032_length_7161_cov_18.538137, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000096 DAOMJU010000000
VERSION     DAOMJU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11032_length_7161_cov_18.538137"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1166)
                     /locus_tag="PLO58_08365"
     CDS             complement(84..1166)
                     /locus_tag="PLO58_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQO74843.1"
                     /translation="MNNTSATPTSQLSKTIWRIFLIIMGVAFIFSIFSYVKTVLLMLL
                     IAWLISVILNPLVDYLESIGFNRALAILTIMAVILGGIIALIIGIIPTVIKAVESISA
                     TLKSDLISNLTIQLEQFFQEKFNNPDLARDLINKAEQIGGTLLVKVAGFLKNAGTYVA
                     FVGIVPIITFFLIKDRRLFKRALISQVPNRYFELFLNVLHKVDSQVTKYIQGQAIDAL
                     IVGLLSVIGLFIINLVFDNPVPYFFVIGMIAGVANLIPYLGPYVGAVPAIVLTILNNP
                     NNVGYIIFWIIVVFVLVQLIDNNLVSPMVVSKSVNMHPITVVIAVIIGGNIAGMFGML
                     FAVPFWGIIKVTMREVSWGLRSYKLK"
     gene            complement(1159..1683)
                     /locus_tag="PLO58_08370"
     CDS             complement(1159..1683)
                     /locus_tag="PLO58_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQO74844.1"
                     /translation="MNHPLWDPISAKNKSEMSDIMQILAKVPIFSDLDSKELKEVEKI
                     IYHRKYKKGEPIFRMGDPGLGMYIIVNGAVEIVEEPENGTRTKLAELEKGAFFGDMAL
                     LDEKPRSASAIAMVDSDIIGFFRPDLVDLLYRKPRMGIKILWALSRVVGERLRKTDEQ
                     LTNLQREMKAKRHE"
     gene            1981..3186
                     /locus_tag="PLO58_08375"
     CDS             1981..3186
                     /locus_tag="PLO58_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQO74845.1"
                     /translation="MLLIRNFLIIIVLLFLSPVQVNSQEKDLDQTIEYQSEQLEKIRK
                     EIRNYKSDLKKAESKEKSILTQIDETERSISLTEKLIAQLNREQIQKEREIEQIERSI
                     KQLESDLNTLKNDFAKRLVYLYKNGKYNDLELILTAKSINQAFYRYKYLRILADIDRR
                     TCTLIRKDINTINQKKQQLNQELRNKEKIINEKKDLMANLQQQKRRHNVQLAKARQNK
                     SNIAAQIKEKEKNAAEISKLIANLDKEREKRNKELERQRALSGVAESNPFLASQGELI
                     WPAKGEIVSRFGIQKHPTLKTITDNPGIDIKVKLGTPVVSVLDGVITTITYIRGFGNT
                     IIIDHGSGFYSVYSHVENIRVAENDYVTANSVIAEVGQSGSLSGPMLHFEIWRNKTKL
                     NPEEWLNKR"
     gene            3183..4313
                     /locus_tag="PLO58_08380"
     CDS             3183..4313
                     /locus_tag="PLO58_08380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HQO74846.1"
                     /translation="MTLFPLINQTENRNLLRNIVATGRVANTYLFYGPEGSGTEGFAL
                     EFAAMLNCSSKSERPCGICQSCKQMKTLEHPNLTLVFPIPGSPDSKPGDSPFKKFKEG
                     EMDEIRQIIQNKSRNPYDKIIVKKALVIPINFIREIKRHIYLAPQNGGQKVVVIFDAH
                     LMNDEAANAFLKILEEPPDNSTIILSTAYPDQLLPTVQSRCQWLYFPPLPEAELKEFL
                     IKKGYPLENIRLAIRLSGGNVSQLTQLIEHDLSDIKELTLAIFLDIAAWKVSNLYNHI
                     QKMTTIYRQDPEKFAQLMQSLIFWIRDAELIRQGQPIDDIIHLDYAERLTNFVKKFPD
                     FDAQNMKVSVENCVDFINKNVYINLALIEMFFKLKKQLNNSK"
     gene            4316..5137
                     /gene="ricT"
                     /locus_tag="PLO58_08385"
     CDS             4316..5137
                     /gene="ricT"
                     /locus_tag="PLO58_08385"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HQO74847.1"
                     /translation="MNTSDKLIKIKFKGGNRQLCQNPLDFPVQPNDKMVVSADRGEEL
                     GCALSSGSPKNLPEELGEIPSLLRKATPQDLSQSNENRLQEKMALEFCVAKISELNLP
                     MKLIDVDVRLDRRKIVFNFTADERIDFRELVKLLAAEFKTRIEMRQISSREEMKRTDG
                     VGLCGLRLCCASFIDDFDPISTQFAKEQNLPMNPAKISGCCGKLKCCYRYEHDFYEEV
                     LKNYPAYGTKVKVADKTGILEKIDIFAQTLTIRYEDNTAEDFPLTDFNQKITTVQ"
     gene            5148..7040
                     /gene="metG"
                     /locus_tag="PLO58_08390"
                     /pseudo
     CDS             5148..7040
                     /gene="metG"
                     /locus_tag="PLO58_08390"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
BASE COUNT         2266 a         1622 c         1330 g         1943 t
ORIGIN      
        1 cgcgcttaaa tgaacttaat tgcttagcgc actctgcggt tggctatttt cttaagattt
       61 acgttgattt atagattgct gagttatttg agtttgtaac ttcttaaacc ccagcttact
      121 tctcgcattg taactttaat aattccccaa aacggaaccg cgaacagcat cccgaacata
      181 cctgcaatat taccaccaat tatcaccgcg atcacgacag ttatcggatg catattgacg
      241 ctcttagaga ctaccatcgg tgagaccaga ttattatcaa tgagttgcac gagaacaaat
      301 accacgataa tccaaaaaat gatatatccg acgttattcg gattattcag aatagtaagt
      361 acaatagcgg gaacagcacc gacatatggc ccgagataag gaataagatt agcgacgcca
      421 gcgatcattc cgataacaaa aaaatacggc accggattat caaataccaa attgataatg
      481 aataagccga ttaccgacaa taagccgacg atcaaagcgt ctatcgcttg cccctgaata
      541 tatttcgtaa cctgagaatc aactttgtgc aagacattta aaaataattc gaaataacga
      601 ttaggcacct gactgattag tgctctctta aataatcgtc gatccttgat caaaaaaaag
      661 gttataattg gtacaattcc aacgaatgct acataagtgc cggcattttt taaaaaaccc
      721 gcgactttca ccagtagcgt gcctccgatt tgttctgcct tatttatcag atcgcgcgcc
      781 agatcgggat tgttgaattt ttcctgaaaa aattgctcaa gttgaatggt caagttacta
      841 attaaatcac tttttaaagt agcggagatg gattcaaccg cttttattac ggtcggaata
      901 atgccgataa ttaaagcaat aatgccacca agaataacag ccataatggt tagaattgct
      961 aaggctcggt taaatccaat actttcaaga taatctacta atggattgag aatcaccgaa
     1021 ataagccagg caattaacaa cattaataac acagttttaa cataggagaa gatcgaaaat
     1081 ataaatgcca ctcccataat aattaaaaat atacgccaga tggttttact taactggctt
     1141 gttggagtag ccgatgtatt attcatggcg tttcgccttc atttcacgtt gtagattagt
     1201 caattgttca tctgtttttc gtaaacgctc accaactact cgggataaag cccagaggat
     1261 tttgattccc atacgaggtt tgcgatacaa taaatccact aaatccggtc ggaaaaaacc
     1321 aattatgtca gaatcaacca tcgcgattgc cgaggctgaa cgtggtttct catcgagtag
     1381 agccatatcc ccaaaaaaag cgcctttttc aagttccgct aatttcgtcc gggttccatt
     1441 ttctggttct tcgacaattt ccaccgctcc attaacgata atatacattc caagccccgg
     1501 gtcacccatt cggaatatcg gctcgccttt tttatattta cgatggtaaa ttattttttc
     1561 cacctctttt aactccttag aatccagatc agagaagata ggcactttag ccagaatttg
     1621 cattatatcg ctcatttccg atttattttt ggctgatatt gggtcccata atggatgatt
     1681 cattttatct attcctttta cttcattttc caataatatc gaggtaatat acaaacaatt
     1741 tatttataca aacaattcca ataagtcaag acttctccaa tatttcctat cctttgcccc
     1801 ctaattattg atgttcaaag ataataaagc aaaaatacaa tttttctcca tcacaattta
     1861 ttcactaaaa aatattaact cgaagaggtt aaaccttcta aaaattggtt gatgtcctgt
     1921 aacaagtgac ttgctattca actcgacatt acttatttta agccgtttat tatgagtatg
     1981 atgttattaa ttagaaactt tctgataatc atagttttac tgttccttag cccggttcag
     2041 gttaactcac aggaaaaaga cctggatcaa acgattgagt atcaatcgga gcaactggag
     2101 aaaatccgta aagaaatccg caattataaa agtgatctta aaaaagccga gtctaaagag
     2161 aaatcaattt tgactcaaat tgatgaaacc gaacgcagta tcagcctgac cgagaaactc
     2221 attgcccagc ttaatcgcga acagattcaa aaagagcgcg aaatcgagca aatagagcgt
     2281 tcaattaagc aattggagtc cgatttaaat actcttaaaa acgatttcgc caagcgcctt
     2341 gtttatcttt ataaaaacgg caaatacaac gacctggaat tgatcttaac cgctaaatcc
     2401 atcaatcagg ctttttatcg ttataaatat ctgcgcattc tggctgatat tgaccgccgt
     2461 acttgtactc ttattcggaa agatatcaat actataaatc aaaaaaaaca acaactaaat
     2521 caagagctaa gaaataaaga aaaaatcatc aatgaaaaaa aagaccttat ggctaatctt
     2581 caacagcaaa agcgtcgcca taatgtccag ctcgcaaaag cccgtcagaa taaatcaaat
     2641 atcgccgccc aaattaaaga aaaagaaaaa aacgccgccg aaatttccaa attgattgct
     2701 aatctggaca aggagcgcga aaaacgtaac aaagagctcg aacgtcagcg cgctctatcc
     2761 ggagttgcgg aaagcaatcc atttcttgca agccagggcg aattaatctg gccagccaaa
     2821 ggtgaaatcg tctcgcgctt tggtattcaa aagcatccta cgctaaaaac aatcaccgat
     2881 aatcccggta ttgacataaa agtaaaactc ggaacgccgg tggtttccgt cctggacgga
     2941 gttattacaa ctattactta tattcgcggc ttcgggaata caatcatcat tgatcacggc
     3001 tccggttttt attcggttta ttcccatgtc gaaaatattc gcgtcgctga aaatgattat
     3061 gttacagcca attcagtcat cgccgaggtt ggtcagtccg gctcactgag cggcccaatg
     3121 ttgcattttg aaatctggcg taacaaaaca aaactcaatc ccgaagaatg gctgaataaa
     3181 agatgacact attccccctt attaatcaga ccgaaaaccg aaatctatta cgcaacatcg
     3241 tcgccactgg cagagtagca aacacctacc tattctatgg cccggaaggt agcggaacag
     3301 aaggcttcgc ccttgaattt gcggcaatgc tgaactgctc ttcaaaaagt gagcgaccat
     3361 gtggtatctg tcaatcttgt aaacagatga aaaccctcga acatcctaac ctaaccctgg
     3421 ttttcccgat tccgggttca ccagactcta aaccaggcga ttcgcctttc aaaaagttca
     3481 aagaagggga aatggacgaa atccggcaaa ttattcagaa taaaagtcgt aatccttacg
     3541 ataaaattat agtcaaaaag gcgctcgtca ttcccatcaa ttttatccgt gaaattaaac
     3601 gccatatcta tctcgctcct cagaatggcg gacagaaagt cgtagtcatt tttgatgcgc
     3661 atttaatgaa cgatgaagcc gctaacgctt tcctgaaaat cctcgaagaa ccacccgaca
     3721 attctactat aatcctctcc accgcttatc ccgatcagct cctgccgacc gttcaatcac
     3781 gctgtcagtg gctatacttc ccgcctttgc cagaagctga actgaaagaa tttctcataa
     3841 aaaaagggta tccacttgaa aatatccggt tggctattcg tctctcaggc ggcaatgtca
     3901 gccagcttac tcaactaatt gaacacgatt tatcagatat taaagaattg accttggcaa
     3961 ttttcctgga tatcgccgcc tggaaagttt ccaatttata taatcatatt caaaaaatga
     4021 cgactatcta tcgccaagac cctgaaaaat tcgcacaact gatgcaatcc ctgattttct
     4081 ggattcgcga tgccgaattg attcgccagg gacagccaat tgatgatatt atccatctcg
     4141 attatgccga aagactaact aattttgtta aaaaatttcc ggatttcgac gcccaaaata
     4201 tgaaggtttc cgtcgagaat tgtgttgatt tcattaataa gaatgtctat attaacctcg
     4261 ccttaattga gatgttcttt aaactgaaaa agcaacttaa taattctaaa taacaatgaa
     4321 taccagcgac aaactcatca aaataaaatt caaaggcggt aatcgtcagt tatgtcagaa
     4381 cccgctcgac tttccagttc agccgaatga taaaatggtc gtatccgccg atcgtggcga
     4441 agaactcggt tgtgccctat catcgggctc acctaaaaat ttaccggaag aattgggtga
     4501 aattccctct ctcttgcgta aagccacccc gcaagaccta tcgcagagca acgaaaatcg
     4561 tttacaagaa aaaatggctc tggaattctg cgtcgctaaa attagcgaat taaatctgcc
     4621 aatgaaatta attgacgttg atgtgcgtct tgaccggcgt aaaattgtat ttaatttcac
     4681 ggcagatgaa cgaattgatt tccgtgaatt agtaaaactc ttagcggctg aatttaaaac
     4741 tcgcatcgaa atgcgccaga ttagcagtcg cgaagaaatg aaacgtactg acggagtcgg
     4801 gctctgcggc ttgagactat gctgtgccag tttcattgat gatttcgatc caatcagtac
     4861 tcaattcgct aaggaacaaa atttgccaat gaatcccgcc aaaatttctg gctgttgcgg
     4921 caaattgaag tgctgttacc gctacgaaca tgacttttac gaagaagtat taaaaaatta
     4981 tcctgcttat ggcaccaaag tcaaagtcgc cgataaaacc ggcattctcg aaaaaattga
     5041 tatttttgct caaactctca ctattcgcta cgaagacaat actgccgaag actttccatt
     5101 aaccgatttc aatcaaaaaa taactacagt ccaataattt tcaccaaact atgaataatc
     5161 aagaacattt ctttgttacg actcccatct attacgtcaa cgacaagccg catattggtc
     5221 acgcttatac gaccattctc gctgatgtgt tggcacgttt tcagcggagt aacggtgtcg
     5281 aaactttctt cttaaccggt gtggatgaac acggccagaa agttcaacaa gccgccgcct
     5341 ctgccggtat ttctcctcag gaatattgtg accgaatggt tgtctatttc cgcgacgcct
     5401 ggcaaaaatt aggaatcaaa tacgactttt ttatccgtac aaccgaccag tttcatgtcc
     5461 gtgctgttca gcaagttcta caagatttgt tcaataaggg tgaaatctac ctgcacgaat
     5521 atggcggcta ttattgcgtt ggatgcgaac gattttatac tcctaaagaa ctggttaatg
     5581 gtcaatgtcc ccagcacttg aaagagccgg attttatcaa agaaaagaat tatttcttcc
     5641 ggatgagtaa ataccaggac tggctaatcg gttacattca agaccatccg aattttattc
     5701 gaccggagag ccgcaaaaac gaagtactgg gctttttgaa aaatccattg ggcgatctgt
     5761 gtatttcccg ccccaaatcc cgcctaagct ggggcatcga attgccgttc gattcgggtt
     5821 atgtcaccta tgtctggttc gatgctttgc tgaattatat tacggcgatt ggctatccga
     5881 acgaccggca acgtttcgca aaatggtggc ctgctgattt tcatctgatt ggcaaagaca
     5941 tcgttacaac tcactgcgtt tactggccaa ctattttgaa agcaatggat ttaccgatgc
     6001 cgaaaaccat cttcgctcac ggctggtgga tgattgacga aaccaaaatg tcaaaatcac
     6061 tcaaaaatat tgtcgagccg ctcgatctgg ttgacgctta tggcgttgac ccggtgcgct
     6121 atttcctgat gcgcgatatg gtgctcggac aggacgccaa tttcagcgaa ggaatcttca
     6181 tcaagcgcta caattccgaa ctggctaatg acttcggcaa tctcgttagt cgcgtgacta
     6241 cgttaataaa taaaaatttt tcgcaaattc cagcgcccga gcaattaagt aatgccgaaa
     6301 acgaaatcca acaaaccgct gagcaattgc ctaacattac tcataaactc ttcagcgaat
     6361 tgaaaataac cgaagcctta gatgaaatca ttaattacat acgatctata aacaaatatc
     6421 tagaagtgcg tcagccctgg cacctcatta aaagcgataa aaccgccgcc ggaaccgtgc
     6481 tttttaccgc gctggaaggc ttgcgtctcg ccgcgacact actcgaaccg attatgccgc
     6541 aaaaaattgc ggaattgttg aacgctctgc gggttgacga aaataaaccg cctttcgaat
     6601 ggggacgaat caagagcggt gtgactctcg gcgagcttcc aattctcttc cctcgcattg
     6661 ataaaattgc caaaccgatg gtcgaaccgc cttccacccc accccaaccg gctgaaccat
     6721 taatcacaat tgatgatttt gccaaaatta aactcaccac cgctaaggtt ctgaccgccg
     6781 aaaaagttcc ggatgccact aaattactaa aattgacaat tgacatcggc acggaaaaac
     6841 gtcaaatcat cgctggaatc gccgagtatt attcaccgga gcaaattatc gggaaaacga
     6901 ttgtcgtcgt caccaatctc caaccggcaa cgattcgcgg cataaaaagc gaaggtatgt
     6961 tattagccgc caaaaaagac ggcaaactga ctttactcag cagtctcgag ccgatcgaac
     7021 cgggcgcaaa aatcagctga tttaatgttc gttgatactc acgcgcatct cacctaccct
     7081 gatctgattg ttaaactcga tgaaattatt aagcgcgctc gcgatgccgg agtcactcgc
     7141 attgtcaaca ttgctaccga c
//
LOCUS       DAOMJU010000097         6907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_11608_length_6907_cov_24.675569, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000097 DAOMJU010000000
VERSION     DAOMJU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11608_length_6907_cov_24.675569"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1234)
                     /locus_tag="PLO58_08395"
     CDS             complement(11..1234)
                     /locus_tag="PLO58_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQO74848.1"
                     /translation="MNFDEGLKLAFQTVKSNKMRTFLTTLGIVIGVTAVIGMMSIINA
                     LDRYMTKSLSSIGGNVFWIQKYPAVQVGHLDQKYRMRKDLKYEHAEAIKRSATLVSAV
                     SAEFFRWGKTVKYKDRATNPNVLVYGGDEYWADVNGRNISEGRMISLMDVQHRRAVAV
                     LGADVAEKLFPFTYPIGEQVKIDGIRYEVIGVMEKMGQAMMGNQDNIVTIPNSTFIKT
                     YGDWGSINIAVKAKSAETILDAMDQVTAILRIARKVPPGDENDFEVVTADSIMNTLRN
                     LTGFIFIAAVIICGISLLVGGIGIMNIMLVSVTERTREIGIRKAVGAKRRHILTQFMT
                     EAVGICLFGGLIGILLGVVVGLLIAQAFKLPPVIPLWSIFVGMGFSLLIGVVFGTYPA
                     LKAAKLDPIEALRYE"
     gene            complement(1348..2577)
                     /locus_tag="PLO58_08400"
     CDS             complement(1348..2577)
                     /locus_tag="PLO58_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQO74849.1"
                     /translation="MISFFIKLWENVKIALRALMQSKMRTILTTLGIVIGVLTVVSVA
                     SIIEGLNRGFASQIANLGSNALYVERYPWATTEDFSKFRNRPRLTLKEADFIRERMRL
                     AEAVSPSTSTRQDIRYRNRTLNGISVNGVSDHYDVVTGLVIEEGRFFSFAEIERNKNN
                     VVLGWEIKDKLFENRNPIGEKIKIGATTYTVIGVTEKRGSFLGESLDAEVYVPLGTVF
                     KNFGWHRSISIAVKISDPARVEEAKDELRFLMRLARKLTPTQEDNFSINQQEVLTDMY
                     NRLTRGLYTAAIGIGTLSLLVGGIGIMNIMLVSVTERRKEIGIRMALGAKRKVITFQF
                     LVESVIICGVGGIIAIILSFIVSLIISKITPFPSAVPAWSVFMGLGFSAVVGLFFGIY
                     PASKAARLNPIECLRYE"
     gene            complement(2582..3265)
                     /locus_tag="PLO58_08405"
     CDS             complement(2582..3265)
                     /locus_tag="PLO58_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQO74850.1"
                     /translation="MDSLIELKDIVKIYKVGSEEVHALRGVNLSIQPNEYVSIMGPSG
                     SGKSTLMNIIGCLDTPTSGRYELEGVPVHEMDDNQLAEIRNRKIGFVFQTFNLLPRVN
                     ALHNVELPLIYSGIPAAKRKILAEAAIEKVGLSDRKNHKPNELSGGQRQRVAIARALV
                     NNPSIILADEPTGNLDSATGEEIMQIFKDLHEAGNTVVIITHEKDIAERAKRIVHIRD
                     GNIAEDIRN"
     gene            complement(3265..4182)
                     /locus_tag="PLO58_08410"
     CDS             complement(3265..4182)
                     /locus_tag="PLO58_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74851.1"
                     /translation="MKKLFILMFGLAFTTFLSAADSTNTNDPFDELKAIPTQDTLRDE
                     LSEEEEKSLDEIVEDENWKSRLPLNDWNWNWEEDDKEAYRRGQKHGYFRGAAGGWDIF
                     YLPLNLDDLNRSLNPIGVTDFDTYMILNGGGGWVFLGKSIRIGGFGAGGEVIAHGKPG
                     DIQKEVKLSISFGGFTIDKVFHPFSKTELYLGLMIGGGSNTINFYQHGTIVSWDSLIA
                     GYTPNTLTTSHTFHDYSSSLSADYFTLMPTVGFRYNIFRWFAVGGNVGYIYTHIDQKG
                     WRMNHYRVEGVPDLNLDNVIYRLNFYFGG"
     gene            complement(4198..>5100)
                     /locus_tag="PLO58_08415"
     CDS             complement(4198..>5100)
                     /locus_tag="PLO58_08415"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQO74852.1"
                     /translation="KALYERNLAPEADMEAAEAQMELAESQVVQAEAALKQALDDLDK
                     TRITAPMAGIITSVKKEVGEIALGSVFQEDVILVVSDMSKVQVEVDVDETDVVNVSIG
                     DTALIELDAIPNTKFKGVVSEIAHSATTTGVGTQEQVTNFLVKIAVLGQDARFRPGMS
                     ATVDIITDVKEDAIAVPIQALTARPPLVKPPHGEQSPTEKTEKSQYPTPTAVPFGQIK
                     TEEVVFVVKSPVGDTTKTNKPLKVSTGPMAEQRAVKIGISSDTHFEILEGLDEGEEIV
                     VGPYKVISKDIKDGSILKITNKIG"
     assembly_gap    5101..5200
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5290..6594)
                     /locus_tag="PLO58_08420"
     CDS             complement(5290..6594)
                     /locus_tag="PLO58_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQO74853.1"
                     /translation="MKRVIFILLIGQILAYGADSLSLKDCINLAISQNQDLKISNKQL
                     QMAEEGIRSTYSAVLPSVSFDYSATKSTVGAGERFYQGAYIPTNDTSSNYFSTGLSLN
                     QNLYNGGKRRNQIKLAKGEAESSRLNLNRTQQAIIANVTEKFYTVLKAKELLKVYEMT
                     LKNSQEQLKKTEEMYKIGQVAKKDILKAQVSEGESRLNLIKQQTSMKNALNDLKMAIG
                     LASDYALDVYEDSYLKPEIIDQAVAEQKALENNPELKSLQIQKSNSYLNYKIAQGDLW
                     PSVNGSFSYGRDGSEIERTYSEFDKWWRTSLNLNISYPLFTGFNRKANIQQKKINYNL
                     YDDQIEKKKLEIISEVERLILTINTNLEMIAINEINILSAQEDLRLAQEMYRLNSATL
                     LDVLDAQVAFTKAQGNLVTTKYDAKIAEVQLVYLMGILQQPE"
     gene            complement(6617..>6907)
                     /locus_tag="PLO58_08425"
     CDS             complement(6617..>6907)
                     /locus_tag="PLO58_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74854.1"
                     /translation="ANLVDIIANAVKVPLMLSQQTVRIYTSPALFLQDNSTFWFRFFA
                     NLDLFVLWKVILLGIGVGIITKVKTSKSIWTILICWIIYCLAVAGLGGLAKV"
BASE COUNT         1816 a         1606 c         1396 g         1989 t
ORIGIN      
        1 ctcagcctct ttactcgtac ctgagtgctt cgatcggatc gagtttagcg gctttcagcg
       61 ccggataagt tccgaaaacc acaccaatta aaagcgagaa acccattccg acaaagattg
      121 accagagagg aattaccggc ggcaatttaa atgcctgggc aattaacaat cccactacca
      181 ctcctaatag aattccgatc aagccaccaa acaggcaaat gccaaccgcc tcagtcataa
      241 attgagtaag aatatgacgt cgcttggcac caaccgcctt gcgaatgccg atttcccggg
      301 tacgttctgt caccgaaacc agcataatat tcataatacc gatcccgccg actaacaatg
      361 aaattccgca gataatcacc gccgcaatga atataaaacc ggttagatta cgcagggtat
      421 tcataatcga gtcagctgtt accacctcga aatcattctc gtctccgggc ggaactttgc
      481 gcgcaatccg taatatcgcc gtcacctgat ccattgcatc gagaatcgtc tctgcacttt
      541 tagctttaac ggcaatatta atggaacccc agtcaccgta ggttttgata aaggtcgagt
      601 tcgggattgt gacgatgttg tcctggttac ccatcatcgc ctgacccatt ttctccatca
      661 ctccgatgac ttcatagcga atgccgtcaa ttttgacttg ctcgccgatt ggataggtaa
      721 atggaaataa tttttccgct acatcagctc ctaatacagc tactgcccga cgatgttgca
      781 catccatcag gcttatcatt cgtccttcgc tgatattgcg cccgttgaca tccgcccaat
      841 actcgtcacc gccgtaaact aacacgttgg gattagttgc tctgtcttta tacttgacgg
      901 ttttgcccca gcggaaaaat tccgctgaaa ccgccgaaac cagggtagca ctgcgtttaa
      961 tggcttcggc gtgttcgtat ttcaagtcct tacgcatgcg atatttttga tcgagatgtc
     1021 ccacctgtac ggccggatat ttttgaatcc agaaaacatt acccccaatg ctacttagac
     1081 tcttagtcat atagcggtcc agagcattta taatggacat catcccaata acggccgtaa
     1141 cgccgataac gattcccagc gtcgtcaaga acgtgcgcat cttattggac tttactgtct
     1201 gaaaagcgag ttttaaacct tcgtcaaaat tcatcttgac ctttttccag gaataaaggt
     1261 ggttttaatt tggtggtcgt ttgtgatcat tcagtggtcg ttcgatagtc attcatttaa
     1321 aaatttggta attacggttt tcatatttta ttcatatctc aagcattcga ttggattgag
     1381 gcgggcggct ttgctcgctg ggtaaattcc gaaaaatagt ccgactaccg ctgaaaatcc
     1441 taagcccata aaaaccgacc aggctggaac agccgaggga aacggtgtga ttttgctaat
     1501 gattaatgag acgataaaac tcaaaataat ggcgatgata ccgcctacgc cgcaaatgat
     1561 tactgattcg accagaaatt ggaaggtaat gactttgcgc ttggcgccga gcgccattcg
     1621 gataccgatt tctttgcgac gttccgtgac tgaaaccagc ataatattca taatgccgat
     1681 tccgccgacg agtagtgaca gcgtcccgat cccgatcgcg gcggtataaa gtccacgagt
     1741 caatctattg tacatatcag tcagcacttc ctgttgattg atcgaaaaat tatcttcctg
     1801 ggttggtgtc aatttgcggg caaggcgcat tagaaaacgc aattcgtctt tggcttcttc
     1861 gactctggca ggatcactta tctttaccgc aattgaaata ctacggtgcc agccaaagtt
     1921 tttaaaaacg gtgcccagcg ggacataaac ctcggcatcg agactttcgc ctaagaaact
     1981 accgcgtttc tcggtcacac cgatcacggt ataggtcgtt gcgccgattt taatcttttc
     2041 gccaatgggg ttacgatttt cgaataattt atctttgatt tcccagccta aaacgacatt
     2101 atttttgttg cgttcgattt cggcaaaaga gaaaaaacgc ccctcctcaa tcacaagtcc
     2161 cgtgaccaca tcataatggt ccgagacgcc attgactgaa atgccattta gggttctatt
     2221 gcgataacga atatcctgac gcgtactggt agaaggcgaa accgcttccg ctaatcgcat
     2281 ccgttcccga ataaaatcgg cttcctttaa tgtcagcctc gggcgattgc ggaatttgga
     2341 aaaatcttcc gtagtcgccc agggatatct ctcgacataa agagcattgc taccaagatt
     2401 ggcgatttga ctggcaaatc cgcgattcaa tccttcaatg atcgaagcca ccgaaacgac
     2461 cgtcagaaca ccgattacga ttccgagtgt cgtcagaata gtgcgcattt tgctttgcat
     2521 aagcgcacgc aaagcaattt tgacattttc ccagagttta ataaaaaagg aaatcacgtt
     2581 ttcaattccg aatatcttca gcgatattgc cgtcgcgaat atggacaatc cgcttggctc
     2641 tttcggcaat gtctttttcg tgagttatga ttacgacggt attgccagcc tcatgcaaat
     2701 ccttaaaaat ctgcatgatt tcttcgccgg tggccgaatc cagattgcca gttggttcat
     2761 cggcaagaat aatagacgga ttattaacca atgctcgagc gattgccacg cgctgacgtt
     2821 gaccgccgga tagttcattc ggtttgtgat ttttgcgatc agaaagacca actttctcaa
     2881 tggcggcttc ggctaatatt ttccttttag ccgccggtat gccactgtaa atcaatggca
     2941 gttccacgtt atggagggcg tttacccgcg gtagtaaatt aaaagtctga aacacgaagc
     3001 caattttgcg attgcggatt tcagccagct gattgtcgtc catttcgtga actggtacgc
     3061 cttctaattc gtagcgaccg gaggtcggag tatccagaca gccgataata ttcatcaagg
     3121 tggatttacc ggaaccggat ggtcccataa tcgagacata ttcgttcggt tggattgaga
     3181 gattcacacc gcgtaacgca tgaacctctt cgctaccgac tttatatatt ttaacaatat
     3241 cttttaattc aattaaagaa tccattagcc accgaaatag aaatttaaac gatatattac
     3301 attatcaagg ttcagatccg gcacaccttc aacgcgatag tgattcatcc gccaaccttt
     3361 ttgatctata tgggtgtaga tatatccgac atttccacct actgcaaacc agcggaagat
     3421 attatagcga aaacctaccg taggcataag ggtgaaataa tcggctgaaa gagaactgct
     3481 atagtcatga aaagtatgag atgtcgttag agtattggga gtatagccgg caattagact
     3541 atcccaagaa acaatcgttc catgttgata aaagtttatg gtattactgc caccaccaat
     3601 catcaaaccg agatacagct ccgttttact aaaggggtga aaaactttgt caattgtaaa
     3661 acctccaaaa ctaattgaaa gtttaacttc tttctgaata tcacccggtt tgccatgggc
     3721 aatcacctct cctccggcac caaatccccc aattcggata ctttttccta aaaataccca
     3781 gccgccacca ccgttcagaa tcatatatgt atcaaagtca gtgacgccga tcgggttaag
     3841 agagcgattc aaatcatcta aattcaacgg caggtaaaaa atatcccaac cgccagccgc
     3901 gccacggaaa tagccgtgtt tctgacccct tctataggct tctttgtcat cctcttccca
     3961 attccaattc caatcgttga gcgggagacg acttttccaa ttttcatcct cgacgatttc
     4021 atccagcgat ttttcttctt cttcagaaag ttcatcgcgg agggtatcct gggtgggaat
     4081 tgctttcaat tcatcaaagg gatcattggt attggtcgaa tcagccgcgc ttaaaaacgt
     4141 agtaaatgcc aatccaaaca ttagaatgaa taactttttc attgctttgc tccttcttta
     4201 tcctattttg tttgtgattt ttaatatcga accgtctttg atatctttgc tgatgacttt
     4261 ataggggccg acaacgattt cctcgccttc atcaagacct tcgaggattt caaaatgggt
     4321 gtcggagcta atgccgattt taacagcgcg ttgttcagcc attgggccgg ttgatacttt
     4381 taaaggttta ttagtcttgg ttgtatctcc gactggagat ttcacgacga acacgacttc
     4441 ctcagtcttg atttgtccga agggaaccgc agtcggtgtc ggatattgtg atttttctgt
     4501 tttttcagtt ggggattgtt cgccatgtgg cggttttaca agcggcggtc gcgccgtaag
     4561 cgcttggatc ggaactgcaa tcgcatcttc ttttacatcg gtaataatat ccacggtggc
     4621 cgacataccg ggtcggaagc gagcatcttg accgagaacg gcaatcttaa ccagaaaatt
     4681 ggttacttgc tcctgggtac cgactccggt cgtagtggcg gagtgggcaa tttccgatac
     4741 gactccctta aatttcgtat tggggatagc atccagttcg attagtgccg tatcgccgat
     4801 tgatacattg acaacgtcag tttcatccac atccacttcc acctgaactt tggacatatc
     4861 cgaaacgacg aggattacgt cttcctgaaa gaccgagccg agggcgattt cgccgacttc
     4921 ttttttcacg ctggtaatga ttccagccat tggagctgtg atacgggttt tgtcgagatc
     4981 gtccagtgcc tgcttcagag cggcttccgc ctggacaacc tggctctccg ccaattccat
     5041 ctgagcttca gccgcttcca tatctgcttc cggcgctaaa ttcctttcat agagagcctt
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttcttattca tattgagcaa
     5221 aacgattgca ccaatgacga taattactat tactgttatt aaaattttct ttccaccttt
     5281 tttcataatt cactccggtt gttgcaatat tcccattaaa tataccagct ggacttcagc
     5341 gattttggca tcatattttg tcgtgaccaa atttccctga gctttggtga aagcaacctg
     5401 ggcatccagc acatccagaa gcgtagccga atttaaacgg tacatctcct gcgccaaacg
     5461 caaatcctcc tgcgccgata ggatattgat ctcgttgatt gcgatcattt ccagattggt
     5521 attaatggtc agaattagcc tctctacttc actgataatt tccagttttt tcttttctat
     5581 ctggtcatca taaagattat agttgatttt cttttgctgg atgttcgctt ttcgattaaa
     5641 gccggtaaaa agtgggtaac tgatattcag gttaagcgaa gttctccacc atttatcgaa
     5701 ttcactgtaa gtacgctcga tttccgaacc gtctcgccca taagaaaaag agccgtttac
     5761 cgacggccag agatcgcctt gcgcgatttt ataattcaaa taggagttcg atttctggat
     5821 ttggagggat ttcaactcgg gattattttc cagcgctttt tgctcggcta cagcctggtc
     5881 aattatctca ggcttgaggt atgaatcttc gtaaacatcc agagcataat cagacgcgag
     5941 accaattgcc attttcaaat cgttcaaagc gtttttcatt gaggtttgct gtttaatcaa
     6001 attcagacgg ctctcacctt cgctgacctg ggctttgagt atgtcttttt tggcaacctg
     6061 tccgattttg tacatttctt ccgttttttt caattgttcc tggctgtttt tcaaagtcat
     6121 ttcatagact ttcaataatt ctttagcctt taaaactgta tagaattttt cggtgacatt
     6181 ggcaataatg gcttgttgag tgcgattgag attcaatcga gaactctccg cttccccttt
     6241 ggcgagtttt atctgattcc gtcgtttgcc gccattgtaa agattctgat tgagggaaag
     6301 acccgtgctg aaataattgg aagacgtgtc attagttgga atgtaagcgc cttggtaaaa
     6361 tcgttcacct gctcccaccg tggatttagt cgccgaataa tcaaaactaa cagaaggtag
     6421 tacagcacta taagtactgc ggataccttc ttccgccatc tgcaattgtt tattacttat
     6481 ttttaagtcc tgattttgag atattgccaa atttatgcaa tccttcaatg acaatgaatc
     6541 ggcaccgtat gccagaattt gcccaattaa taaaataaat attactcttt tcatagctac
     6601 caacctaaaa cctatgttat actttggcta agccgcccag accggcaacc gccagacaat
     6661 aaatgatcca gcaaatgagg atggtccaga tactcttact ggttttcacc ttagttatta
     6721 ttcccacacc gatacccagt aaaatcactt tccagagaac aaaaaggtca agattggcga
     6781 aaaacctaaa ccaaaaagtg gagttgtcct ggagaaataa agccgggctg gtataaatcc
     6841 gcacagtctg ttgagataac ataagtggaa cttttaccgc attagcgata atgtccacca
     6901 gattggc
//
LOCUS       DAOMJU010000098         6826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_11809_length_6826_cov_16.044750, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000098 DAOMJU010000000
VERSION     DAOMJU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11809_length_6826_cov_16.044750"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1413)
                     /locus_tag="PLO58_08430"
     CDS             complement(7..1413)
                     /locus_tag="PLO58_08430"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQO74855.1"
                     /translation="MNSILIVEDSQDTRENIAELLTMNGYKVRTTDNGQTALRIIKTQ
                     MPDCVILDVRLPDTTGHEILKQIQNEIESGLVVIIITAYGEVSMAVNAMKMGAYDYLE
                     KPFENAVLLMVVKRGLENQQVKRELAHLRQMLGTDQDSEQVLGHSPAIKKVISQINSV
                     AQTDLTVLIQGETGTGKEVAAHLLHNKSKRAKQPFIAIDCGAIPENLIESEFFGFEKG
                     AFTGATQSRPGKFQLAHQGTLYLDEIGNLPLAQQRPFLRVIENKTFRPIGAINESKVD
                     IRLIIASNANLLQLVSENKFRKDLYYRISEYTITMPPLRERLEDIPHLTALFLSEANF
                     ELNSKITDISEEAMAKLLSYNWPGNVRQLRNVIRKAALLATDTIKLSHISFPSEFDSE
                     EPEQSFFTVYKNDINRYPNYRTAYAEFTREFEKELIKNAYEKAHGNITKAARIYGIDR
                     RNFYHKLDKYAKDFPKKQ"
     gene            complement(1448..5869)
                     /locus_tag="PLO58_08435"
     CDS             complement(1448..5869)
                     /locus_tag="PLO58_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQO74856.1"
                     /translation="MRSIFKKPEINNEKPAVLRGGRFSRLLILNISTCLLITNAQISI
                     AKTVIKPGKRLHFEKINTEKGLPSNHITSIVQDSIGFMWFATRENGLCRYDGNEIVTY
                     HPEDSVATSLTCQNIKRLFIDSKGALWIIGSVGDIPHKGCIDKYDPLTNGFIHILSPN
                     DSIPSIIDIAEDRLGNFYFSVTDIRQQNNQILKAFNQNGSYQIKKISPLVFNDNSVLP
                     ANYVIHRIINKGDGKLLLGLSQYGIVEYDINTGRVNEDDFQNLNYYFNRFSFKIDDIL
                     LDCNQNLWIAAHKFLAKYSIADGSLQYYLLPLKPDGSEINPYQDPIKNYYYYTINQLY
                     EGSGGQIWVVGSQCNQNETFFRYDPETEIFWHYSTDPYDENQISVNNINCFFIDKSGS
                     AWLATQYSGVFRFSLPRPFMDLRSISDSINFNNDNVNDVLEDHENNLWFGLKGKVLRY
                     NPKTKNVKIFPLKLSNQESPPPGSLHIYEDSQNTIWVGTTLGLFKLNPSSQEFIYLNH
                     VTKNQPNKQNSIRANHIPAIYEDHQGNFWLGTAAGLCRYDRQTNQRKYYSISEPTKNV
                     PNKNIIYDILEDRNNNLWLATRNGLILFDRKTGTYQLISDSTLIFMPESDEGFYRLYE
                     AQDGQIWLTSSLPYLISYNPLTKKFVYRTENSGYIWKYPITGVIEDHNGIFWCRTTQG
                     IIKHNPILNTFTLFDRNDNVNYYYKDRSTSDIREPSYLKNNILRNYCRLRSGWIMIGG
                     KCGYTYFHPDSINNKYVPNIVITKFKNDYTEKIISNHSPNQITLDLPWNENYFTISFS
                     LLDYYIPAENKYYYILEGYDKDWKKCVKTNSVSYSKVPPGQYTFRVKGENSDGVLSRN
                     EATIRIKIVPPFWKTAWFKILIWVLGLGVVVLINYIRIAVIRHQKEELEKQVVDRTQA
                     LIEKSKALEAAHNQLEEKVQERTQELAEANVELNREINIRMKAEESLKRSEEVARVLL
                     NAPIDLAMLIDPDGNILAANNSVLKHFQASENEVINHNLSEFSPPNLFEHQKIFLDKC
                     TNTQSPQEFEDENRGRIFHYHFYPIIGMDQKVNNIAIYIRDITDQREMENFLRNARSE
                     LEKQVNQRTAELRKTNRQLLKEINTRKKIENKLRASEEKYRDLFQNANDLIWTMDKTG
                     KFLSVNNHFKNLSGYSKKELFAINPLTLIHPEHQFRIIRCYLKVLTDGPTGTELNVMT
                     KSGEIRPIWLKMRPIMKNNQIIGIHGIGRDITEIRHAQQELRESEEQKRESMRQFILK
                     LAHEVKNPLASIKSSAQLVASMNNNHNPQVEKHMDIINRNVDTCNKVIRDLFSYAHAT
                     NYQFEEVNAAKFIKSINDFIEEKLNEYPYIKISVNYDRDLPPIYIDEFRLTQAFQNII
                     NNAFEAMTKKGRFTLNVKALKTEKQVQFLFTDTGSGIPAEDLPQIFQSFYSSKSKGFG
                     LGLALTKEIIEFHHGKISVNSILHHGTTFKVTIPAVP"
     gene            6064..>6826
                     /locus_tag="PLO58_08440"
     CDS             6064..>6826
                     /locus_tag="PLO58_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQO74857.1"
                     /translation="MHQTIKIFLIALTLPTLILAGDLFFSEYIEGSSYNKAIEIYNPT
                     GAEVDLSDYTIKQSNGGSGWGCFTSEGGAPTEDTRYILPLSGTLSPDDVYVIYHNDAA
                     SEIKSVGDIGFSYVQTPNGGNGDNVINFNGNDALGLFKDGVLIDVIGVPTENPGEDKG
                     WTVAGTSNATKDHTLVRKSTVSQGNTDWTSSAGTSADDSEWEVHPQNTFTYLGSHVWS
                     GGGNVSPMARAGLDRVERFNVTVTLDGSISNDPDGS"
BASE COUNT         1882 a         1206 c         1475 g         2263 t
ORIGIN      
        1 attgggttac tgttttttgg gaaaatcttt agcatatttg tcgagtttat gatagaaatt
       61 acgacgatca atgccataaa tacgggcggc tttggtaata ttgccatggg ctttctcgta
      121 tgcattttta attaattcct tttcaaattc ccgggtaaat tcagcgtaag ccgttcgata
      181 atttgggtag cgatttatat cgtttttata taccgtaaag aagctttgct ccggctcttc
      241 cgaatcaaat tcagagggga atgaaatgtg agagagcttg atcgtatcgg tagcgagcag
      301 ggcggcttta cgaataacgt tgcgcagttg ccggacattg ccaggccaat tatatgaaag
      361 taatttagcc atcgcctctt cggaaatatc agtgatttta ctgtttaatt caaaattagc
      421 ttcggataaa aataatgcgg ttaaatgagg aatgtcctca agtctttcac ggaggggagg
      481 catcgtgata gtatattccg agattcgata atataaatct ttacggaatt tattttcact
      541 gaccagctga agcagattgg cattgctggc gatgattagg cgaatatcaa ctttgctttc
      601 attgatggca ccgatcgggc gaaaggtctt gttttcgatg acacggagaa acggccgttg
      661 ttgtgcaagg ggtagattgc cgatttcatc gagataaaga gtaccttgat gagccagttg
      721 aaatttcccc gggcgacttt gggtagcacc ggtgaacgcc cctttctcga aaccgaaaaa
      781 ttcgctttcg attaaatttt ctggtatggc gccacagtca atcgcgataa atggttgttt
      841 ggcgcgcttg cttttattgt gtaatagatg ggcggcgacc tctttaccgg ttccggtttc
      901 accttgaatc aggacagtta agtcggtctg agcaacggaa ttgatctgac tgataacttt
      961 tttaattgcc ggactatgac cgagaacctg ttcgctgtct tgatcggttc ccaacatctg
     1021 gcgtaagtgg gcaagttctc gcttgacttg ttgattttcc aagccgcgtt ttacaaccat
     1081 taataaaacc gcgttctcga acggtttttc gagataatcg taagctccca ttttcatggc
     1141 gttgacagcc attgagactt cgccgtaagc agtgatgata ataacgacca gaccactttc
     1201 gatttcattc tggatttgtt ttagaatttc gtgtccggtt gtatccggca ggcgtacgtc
     1261 caggattaca caatccggca tttgggtttt aattatccga agagcggttt ggccattgtc
     1321 agtagttcga actttatagc cgttcatagt taaaagttcg gcaatgtttt cgcgagtgtc
     1381 ttgagaatct tcgactatta atatagaatt cataataaca tcctgatttt aagttatttt
     1441 aaacacttta cgggacggct ggaatagtta ctttaaaagt ggtgccatgg tgtaaaatac
     1501 tatttacgga aattttacca tggtgaaatt cgataatttc cttggtgagt gccagaccca
     1561 gtccgaaacc tttggacttg gagctgtaaa atgactgaaa aatctgcggc aagtcttcag
     1621 ctggtattcc tgagccggta tcggtaaaca gaaattgaac ctgtttttcg gtttttaagg
     1681 ctttgacgtt gagagtgaat cgccctttct tagtcattgc ttcgaacgca ttattgatta
     1741 tgttctgaaa agcctgagtt aatcggaatt cgtcaatata gatgggcggt aagtctcggt
     1801 cgtaattcac agaaattttt atatatgggt attcgtttaa tttttcttcg ataaaatcgt
     1861 ttatggactt gatgaattta gcggcattta cttcttcaaa ttggtaattt gtggcatgcg
     1921 cataggagaa taggtcccga atgactttat tacaagtatc aacgttgcga ttgataatat
     1981 ccatatgttt ttcaacctga ggattatgat tgttattcat actggcaact aactgagcag
     2041 aacttttgat gcttgccagc ggatttttga cttcatgagc tagtttcaag ataaattggc
     2101 gcatactttc gcgcttttgt tcttcggatt cacgaagttc ttgctgggcg tgtcgtattt
     2161 cggtaatatc ccgaccaatt ccatgaattc cgatgatctg gttatttttc attataggac
     2221 gcatttttaa ccagatgggg cgaatttcac cggatttggt cataacattt agttctgtac
     2281 cagttggacc atctgtaaga actttcaaat aacaccgaat tatgcggaat tgatgttcgg
     2341 gatggattaa agttaaggga ttgatggcaa acaattcttt tttagagtag ccggataagt
     2401 ttttaaagtg gttgttaacc gatagaaatt taccggtttt atccatagtc cagatgagat
     2461 cattggcgtt ttggaatagg tcgcggtatt tttcttcact ggcgcggagt ttgttttcga
     2521 tttttttacg cgtattgatt tctttaagga gttgtcggtt ggtttttctt aattccgcgg
     2581 tgcgctgatt gacttgtttt tcgagttcag atcgagcatt gcgcaggaaa ttttccattt
     2641 ccctctgatc ggtaatatcg cggatataga tggcgatgtt gttgaccttc tggtccattc
     2701 caataatagg ataaaaatga tagtgaaaaa ttcgtccccg gttttcgtcc tcaaattcct
     2761 gaggcgattg ggtattggta catttgtcga ggaatatttt ttggtgttcg aatagatttg
     2821 gaggtgagaa ctcacttaga ttatgattga tgacctcgtt ctccgaggct tgaaaatgtt
     2881 tcaaaaccga attgttagcg gctaagatat taccgtcagg gtcaattaac attgccagat
     2941 caataggagc attgagcaga acgcgagcga cttcttcact gcgttttaag gattcttcgg
     3001 ctttcatccg aatattgatt tcgcggttta attccacatt ggcttcggcg agttcctggg
     3061 ttctttcctg gactttttct tcaagttggt tatgagccgc ctcaagggct ttggattttt
     3121 caatcaaggc ttgagtgcga tcaaccacct gcttttctag ttcttccttc tgatggcgga
     3181 taaccgcgat gcgtatgtag tttatcaaaa caacaacacc taaaccaaga acccatatta
     3241 gtattttgaa ccaagctgtt ttccagaaag gtggaacaat tttaatcctg atagtggctt
     3301 cattgcgact taaaacgcca tccgaatttt cgcctttgac tcggaaagta tattgaccgg
     3361 gtgggacttt agaatagcta acgctattag ttttaacgca ttttttccaa tctttatcat
     3421 atccttctaa aatgtagtag tatttatttt cagcggggat atagtagtcc agtaaactaa
     3481 aactgatggt gaagtaattc tcattccaag gtaggtctaa tgtgatttgg ttaggagaat
     3541 gattggaaat tattttttca gtatagtcat ttttaaattt cgtaataacg atattgggca
     3601 catatttatt attaatacta tccggatgaa aataagtata gccacattta ccgccaatca
     3661 taatccagcc ggaacgcaat cggcagtaat tccttaagat attatttttt aggtagctgg
     3721 gttctctgat gtcagaggtt gatctgtctt tgtaatagta atttacattg tcattgcgat
     3781 cgaagagggt aaaagtattc aatattggat tgtgttttat aattccttga gtggttctac
     3841 accagaaaat gccattgtga tcttcaatta cgccggtaat tgggtatttc cagatatatc
     3901 ccgaattttc ggtacgatag acaaatttct ttgtcagcgg attgtatgaa atcaggtatg
     3961 gtaacgacga ggtcaaccaa atctgaccat cttgagcttc gtataaacgg taaaaacctt
     4021 cgtcactttc cggcataaaa atcaaagtac tgtcagaaat taattgataa gtaccggttt
     4081 ttcggtcaaa taaaatgagt ccgttgcgag tcgctaacca taaattattg ttccgatctt
     4141 ccagaatatc gtaaatgata tttttgttcg ggacgttctt agtcggttca gaaattgagt
     4201 aatattttct ttgattggtt tggcgatcgt agcggcataa accagcggcg gtacccagcc
     4261 aaaaattacc ctggtgatct tcatagatag ccggtatatg gttggctcta atcgaatttt
     4321 gcttattcgg ctgattttta gtgacgtgat ttaaatagat aaattcttga gaagatggat
     4381 tgagtttgaa taatccaaga gtagtaccca cccagattgt attttgagag tcttcataaa
     4441 tatgtaatga gcccggtggt gggctttctt gattggagag ctttaggggg aaaattttga
     4501 cattcttagt tttcggattg tagcgcagaa cctttccttt tagaccaaac cagaggttat
     4561 tttcgtgatc ttcaagaaca tcgttcacat tgtcgttatt aaaattaatg ctgtccgata
     4621 ttgaacgtaa atccataaaa gggcgtggta aagaaaatct aaacacacca ctatattgag
     4681 tagcgagcca ggccgaaccg cttttatcaa tgaaaaaaca gttaatatta ttaaccgata
     4741 tttgattttc atcataaggg tcggtcgaat aatgccagaa tatttctgtt tccggatcat
     4801 atctaaagaa ggtctcgttc tgattgcatt ggcttccaac tacccatatt tgtccgccac
     4861 ttccctcata taattgattg atggtatagt aatagtaatt ttttatcgga tcctggtagg
     4921 gatttatttc actgccgtcc ggtttgagcg gcagtagata atattgcaat gagccgtcgg
     4981 caatggaata ttttgccaga aatttatggg cggctatcca caggttttga ttacaatcga
     5041 gtaatatatc atcaatttta aaagagaaac ggttgaagta ataattaaga ttctggaagt
     5101 cgtcttcgtt aacccggccg gtatttatat cgtattcgac tatgccgtat tgacttaaac
     5161 ctaacagtaa ttttccatcg cctttattta ttattcgatg aattacataa ttggcaggca
     5221 atacagaatt atcgttaaat actaaaggac tgattttttt tatctggtat gaaccatttt
     5281 gattaaaggc tttcaaaatt tggttatttt gttgtctgat atcggtgacc gagaaataga
     5341 aattacccaa tcgatcttcg gctatatcaa taatgctggg aatcgaatcg ttcggcgata
     5401 ggatatgaat gaaaccgttg gtaagcggat cgtatttatc gatacaacct ttatgaggga
     5461 tgtcgcccac tgaaccgata atccagagcg caccttttga atcaataaat aatcgcttaa
     5521 tattttgaca ggtgagagaa gtggctactg aatcttcggg atgataagta acaatctcgt
     5581 tcccatcgta gcggcacaat ccattttctc tggtggcaaa ccacataaat ccaattgaat
     5641 cctgaacaat ggaggtgata tggttactgg gtagtccttt ttcagtgtta atcttctcga
     5701 aatgcaagcg tttgccgggt ttgatgaccg ttttagcaat tgatatttgg gcatttgtga
     5761 ttaataaaca agtagatata tttagaatca ataacctcga aaagcggccg cctcttagga
     5821 cggctggttt ctcgttatta atttccggtt ttttaaaaat acttctcatc acgttaagcc
     5881 gaattatctt ccaaccagct taatttaaaa ataattttga ggatgtaaag atagattgta
     5941 actttctaag tgatttttat tcaaattaaa gtggctaaaa atcagaaaac caaccagcaa
     6001 aattatttaa aaactcttat tagccaacgt tcaaaaggta tttaaaaaat tagagaggtt
     6061 attatgcatc aaaccatcaa aattttcctg atcgctttga ctttgccaac cctgattttg
     6121 gccggtgatc tgttcttcag cgaatatata gaagggtcta gctataataa agctattgag
     6181 atatacaatc ctacaggggc tgaagttgat cttagcgatt atactattaa acaatctaac
     6241 ggtggttcag gctggggatg ttttacgtct gagggtggag cgccaacgga agatacgcgc
     6301 tatatacttc cattaagcgg tactctaagt cctgacgatg tttatgttat ttatcataat
     6361 gatgccgctt cagagattaa aagtgttggt gatattggat tttcatatgt tcaaactccg
     6421 aatggtggaa atggagacaa tgtaattaat ttcaatggta acgatgctct gggattattt
     6481 aaagatggtg tattgattga tgtcattggt gtacctacag aaaaccctgg tgaagataaa
     6541 ggctggacag tcgccggtac ttctaatgct actaaagacc acaccttggt tcgtaaatct
     6601 actgtatccc aaggtaatac cgattggacc tcttcagccg gtacttcggc cgatgattcg
     6661 gaatgggaag ttcatcctca gaatacattt acttatttag gttcgcacgt ctggagtggc
     6721 ggtggtaatg tttctcctat ggcacgagcc ggtttagacc gggtggagcg ttttaatgta
     6781 actgtgactt tagatggttc gatcagtaat gatccggacg gttcta
//
LOCUS       DAOMJU010000099         6794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_11887_length_6794_cov_22.382401, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000099 DAOMJU010000000
VERSION     DAOMJU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11887_length_6794_cov_22.382401"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLO58_08445"
     CDS             complement(<1..626)
                     /locus_tag="PLO58_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HQO74858.1"
                     /translation="MSNNKLDIKSLESWLWEAACKIRGPVDAPKYKDYILPLIFLKRL
                     SDVFEDELERLAQNYGSKETVEELLAQDHKLVRFYLPKNARWEVIAKHTTQIGEHLTD
                     AVRLIAKENPKLQGVVDTVDFNATTSGQRILGDDVLKELINVLSKYRLGLNDVDADIL
                     GRAYEYLLRKFAEGSGQSAGEFYTPGEVAILMSHILDPEPGNEIYDPC"
     gene            complement(958..1332)
                     /locus_tag="PLO58_08450"
     CDS             complement(958..1332)
                     /locus_tag="PLO58_08450"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQO74859.1"
                     /translation="MIYGIGIDLIEVERIARQLIGKTHFREKIFSPNEIAYCEEKSNQ
                     AEHYAARFAAKEALFKALGTGWRGGLNFTEVEVLNDDLGKPEFNFLGKTSQIIEELNL
                     THIHLSLSHLAQIAVAVVILEK"
     gene            complement(1360..2319)
                     /locus_tag="PLO58_08455"
     CDS             complement(1360..2319)
                     /locus_tag="PLO58_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HQO74860.1"
                     /translation="MPKILITGALGQIGSELVPALCQRYGSRNIIASDIRPASDQTIL
                     SAATFETLDVTNFNQIAEIVNKYQITTIYHLAALLSAVAEAHPQKAWHINMNGLYNML
                     EVARQYHCAIFMPSSIAAFGASTPRYMTPQDTIQRPDTIYGVTKVAGELLCDYYFKRF
                     GVDTRGVRYPGIISSKTPPGGGTTDYAVHIFYAALKQKHYTCFLKPGTYLDMMYIPDA
                     IKAAINLMEADPARLQHRNAFNVTAMSIDPEMLAAEIRKHIPDFTMDYAIDEVRQRIA
                     DSWPSHIDDSAARAEWDWRPDYNLETMTTDMLKRLSVKLKNTK"
     assembly_gap    2457..2556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2925..>4246)
                     /locus_tag="PLO58_08460"
     CDS             complement(2925..>4246)
                     /locus_tag="PLO58_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HQO74861.1"
                     /translation="QNGALVIFSPNRSDYPIINLPGKGFTTQPLIYGEKLENVAITGE
                     GIFDGCGENWRPVKKFKTTASQWQELLKSGGVVNHSGDIWWPSIEAMQGEHYRDSLLA
                     TKPKNELVAEDFLPARDFLRPELVLLNNCKRVLVDGPTFKNSPKWAFHTQRCENIIIR
                     NVKINNEWWAQNGDGIDISSCKNVLIYKCTVTAGDDAICMKSSKTKGQTEPSLQNVVV
                     RDCIVYRGHGGLVIGSNTDGGMQNIFVDNCIFIGTDIGLRFKSARDRGGLVENVIIRN
                     IYMKDIVNEAILFNTYYENIGREEKTYPVTEKTPIFTNFRLDSIFCFGARQAIGVVGL
                     PEMPVHRIVIRNSILSADVGVTAENARDFILENVNIIPKTEPILHLTQCANFHLDTIG
                     FPATISTFMTLSGKNTQAIHIKNTALSSLKNPIIFSKEVNTNSVIIE"
     assembly_gap    4247..4346
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4347..>6794)
                     /locus_tag="PLO58_08465"
     CDS             complement(<4347..>6794)
                     /locus_tag="PLO58_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide lyase family 1 protein"
                     /protein_id="HQO74862.1"
                     /translation="PFFTSMILNWLIIGQLLAQTSATSTWPLTANQNASVTGNLCAAP
                     QTLNNMQVKYESGVQRSSPSGTAGSWPEETSENINRFMQFAVTPVEGYIFNVNSIEMK
                     LYVNSGSNMRANVYYATDPLFVTKTQIGSTYTLSSSVPSAPNITATLNCEVAYGDTFY
                     VRIYPWYTTSTTGKYIITNSVVISGTTIPSTYMLASPAALDGFIQENAAIPSSAQTYL
                     LSGKNLIDSVTVDPPSNFEISADNGSTWISSGDSLRLPVLDGDIVGQPLSIAVHLKAD
                     SSGEYSGTIEHKSDGATTTSLNVSGAFLANEPTAASVISPDSVTGKTATLSFSGGDGA
                     RRVLTIRPGTEIAWLPEDGKPVRGVSSIFTEAIDQGDGTRIVYDGEDTTVTVTGLTGN
                     TTYTAAIFEYNVATGNTYNYLTTQYGIFTFTTAIVPELSVSPIVLNFGSVLISQKLIK
                     SYTLTGRHLIENDVIHIIAPPDFDLSLAPDTGFVPSLPIAYTTFSFDTTVYVRFAPTE
                     SGTFSDLITNILGNDTVTLTVSGRGVYSLVPTAEPVGFATLNGGASGGQGGDSVIVTT
                     AEHFYELMHARENKSTAPLIVHISGTLSGYSSKIAIKRTANISIVGLDSDAGLNGFGI
                     KIAECNNIIVRNLTFADCHVDEKDALEIDSCQNVWVDHCTFTDSPASDPSGSNHDGLL
                     DIKNGSRNITVSYNYFTNHRQTCLLGHTESQPTDTVMTVTYYRNWFDGTYSRHPRIRF
                     ARAHIVNNLYTDIGSYGVGVTCNAQVLVEANHFENTPTPILISQVNDPNRTLSGDPAG
                     YVKALDNLTINSGAIVE"
BASE COUNT         1755 a         1425 c         1571 g         1843 t
ORIGIN      
        1 cagcagggat cgtagatttc attaccaggt tcaggatcga gaatgtgcga catcaggatg
       61 gcaacctcgc cgggagtata gaattcgccc gcgctctgac cggaaccttc ggcaaatttt
      121 cgcagcagat attcgtacgc tctccccaga atgtcggcat caacatcgtt caagccgagt
      181 cggtatttgc tcagcacgtt gataagttcc ttcaatacat catcgccaag aattctctgc
      241 ccggaagttg tagcattgaa atcaaccgta tccaccacgc cctgcagttt cggattttct
      301 tttgcaatga gtcgcacggc atccgttaga tgttcgccaa tttgcgttgt atgttttgca
      361 atcacttccc atcgagcgtt tttgggcaaa taaaaacgca cgagtttgtg atcctgagcg
      421 agcaactctt cgactgtttc tttcgagcca taattttgcg caagtcgctc gagctcatct
      481 tcgaatacat ccgagagtct cttcaagaag atgagcggta aaatgtaatc tttatacttt
      541 ggtgcgtcaa ctggtccgcg aatcttacac gcggcttccc agagccagct ttccagagat
      601 ttgatatcga gtttgttgtt tgacatagta aagtccttat attaaataga aatcttctaa
      661 ggctttcatt ttgttttcta tttttagcaa atagaacgga gttgctcgat gagagttcat
      721 agcagacata atttcgatta acggtttcac ttttttaatg acaaaattta atcggctaac
      781 tgaaataaaa ccagttaagc aactttttat agattgttgg cccattattt aaatttttac
      841 ctattaagaa tcgccatctc gatgaatttt ttctgctagg cgtaatgacc tgaacattaa
      901 ttattttctc aaaaattaat acggttaccg aagaaggaaa cttcaaataa gccgacgcta
      961 cttttcgaga atgacgaccg ctacggcgat ttgtgccaga tggctcagcg aaagatggat
     1021 gtgggtcagg tttaattctt caatgatttg gctggttttt cccagaaagt tgaactctgg
     1081 cttgcccaga tcgtcgttaa gtacttctac ttcagtgaaa ttcagcccgc cgcgccagcc
     1141 ggtaccgaga gctttgaaaa gagcttcctt ggcggcaaaa cgggcggcat agtgttcagc
     1201 ctggttggat ttttcttcac aataagcgat ctcatttggg gagaagattt tctcgcggaa
     1261 atgagttttg ccgattaatt gccgggcaat gcgttcgact tcgatcaggt ctattccgat
     1321 tccataaatc ataattggaa tgtaaaatga aaaattgaat tatttagtat tctttaattt
     1381 aaccgaaagc cgctttagca tatcagtggt catcgtctcg aggttatagt ccggacgcca
     1441 atcccactcg gcgcgggcgg cagagtcatc aatatggctt ggccaggagt ctgcgattct
     1501 ctgtcgaacc tcatcaatgg catagtccat cgtaaaatcc ggaatatgtt tgcggatttc
     1561 ggcggcgagc atttccggat caatgctcat agcagtaacg ttgaaagcat tgcgatgttg
     1621 caatctggct gggtcggctt ccattaaatt aatagcggct ttgatagcgt ccgggatata
     1681 catcatatcc agataagttc ctggtttcaa gaaacaggta tagtgtttct gtttaagagc
     1741 cgcataaaaa atatgtacgg cataatcggt tgtccctcct ccaggtggag ttttagaaga
     1801 gataatcccg ggataacgga cgccacgcgt gtccacgcca aaacgtttaa agtagtaatc
     1861 gcaaagtaat tccccggcaa ctttagtaac tccgtagatt gtgtcgggac gttgaatagt
     1921 atcttgagga gtcatataac gcggtgttga tgcgccaaat gcggcgatgc tactgggcat
     1981 aaaaatggcg caatgatatt gtcgggcaac ttccaacata ttgtaaagtc cgttcatatt
     2041 gatgtgccag gctttttgag gatgggcttc ggctacagcc gacaatagag ccgccagatg
     2101 ataaatggta gttatttgat atttattaac gatctcggca atttgattaa aattcgtgac
     2161 atcaagcgtt tcaaaggtcg cggcgctaag aatagtttgg tcacttgccg gccggatgtc
     2221 agacgcgata atattgcggg agccatatcg ctggcagaga gctggaacca gttccgagcc
     2281 gatttgcccc agcgcgccgg taattaaaat tttcggcatt ggcacctcca tttgaaaaag
     2341 acggctcgcc tgaattgcgg cgagccgtct aaaaatccct caatccactt tttatttagc
     2401 aattaattaa gtcgcttaaa tacacatcaa tagtcacggg tataatttct taaaaannnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttaa gtgcttataa gaaataaaaa
     2581 tgttactgaa agataaaccg caaagcacgc aaagaattta aatctattca agattctaca
     2641 aatagttcgc ccttacagag cttaataatg aaaaggttta ggctgagagg agaccggtta
     2701 ccggaagacc tgttacccat ttcaggttaa gcatagctca cgaattctcc gaaggccccg
     2761 gaggagtttc tggtaagaga gttactcacg agcgggacta gtttcaccaa gaaattaaaa
     2821 cagttttaaa gtcttaatag aacttataag gacttagtag cacttattag cacttcctta
     2881 caatcatgca accaagtgcc cttaagacaa ctgatattct ccgcttactc gataattacc
     2941 gaattggtat tcacttcttt ggaaaaaatt atcgggtttt tcagagaaga tagtgccgta
     3001 tttttaatgt gaatggcttg cgtattttta ccggataggg tcataaaggt agaaatcgtt
     3061 gcggggaatc cgattgtgtc aaggtgaaaa ttggcgcatt gagttaagtg cagaatcggc
     3121 tcggttttag gaatgatatt tacattttcg aggatgaaat cgcgcgcatt ttcggctgta
     3181 acgccgacgt ctgccgatag aatagaattg cggattacaa tccggtgaac gggcatttcc
     3241 ggtaatccca ccacgccgat ggcttgccgg gcgccgaaac aaaagatgct gtcgaggcga
     3301 aaattggtaa aaatcggggt tttttcggta actggatagg ttttttcctc gcgcccgatg
     3361 ttttcgtagt aagtgttgaa taaaattgct tcgttgacga tgtctttcat ataaatatta
     3421 cggataatga cattttctac caagccaccg cgatcgcggg cgcttttaaa gcgcagtccg
     3481 atatcggtgc caatgaaaat acagttgtca acgaagatat tctgcattcc accgtcggta
     3541 ttactgccga tgactaatcc gccgtgccca cgatagacga tgcaatcgcg cacaacaacg
     3601 ttctggaggc tgggttcggt ttgtcctttg gttttactcg atttcataca aatggcgtcg
     3661 tcaccggctg tgacggtgca cttatagatt aagacatttt tgcaggagct aatatcaatg
     3721 ccgtcgccgt tctgagccca ccactcgtta ttaattttga cgttccgaat aataatgttt
     3781 tcacaacgtt gggtatggaa tgcccatttg ggtgaatttt tgaaagttgg gccatcaacc
     3841 agaactcttt tgcaattatt taatagtacc agttcggggc gcagaaaatc acgcgcgggc
     3901 aagaaatctt ccgccactaa ttcatttttc ggtttagttg caaggagcga atcgcgataa
     3961 tgttcgcctt gcattgcctc tattgagggc caccagatgt cgccggagtg attgactacc
     4021 ccgccagatt tcaacagttc ctgccattgt gaggcagtcg tcttaaattt tttaaccggg
     4081 cgccaatttt caccgcaacc gtcgaaaatt ccctcgccgg taatagctac attttccagt
     4141 ttttctccgt aaatcaaggg ttgggtagta aaacctttgc ccggcagatt gataatcggg
     4201 tagtcgctac gattgggact aaaaattacc aatgccccat tctgcannnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnntttt cgacgattgc cccggaattt atcgtcaaat
     4381 tgtcgagcgc cttcacataa ccggctggat cgccactgag agttctattc gggtcgttta
     4441 cctgagaaat taatataggc gtgggcgtat tttcaaagtg atttgcctcg actaaaactt
     4501 gagcgttaca agttacccca accccatagc tcccgatgtc ggtgtagaga ttatttacga
     4561 tatgagctct ggcaaagcgg atccgtggat gacgcgaata agtgccgtcg aaccagttac
     4621 gataataggt cacagtcata acggtgtcag taggctgact ctccgtgtgt cccagtaggc
     4681 aggtttggcg atgattggta aaataattat aagatacggt aatattgcgg ctaccgtttt
     4741 ttatatccag caagccgtca tgattactac cggacggatc gctggctggg ctatcggtaa
     4801 aagtacagtg atcaacccat acgttctgac aactgtcaat ctcgagcgca tctttttcat
     4861 caacgtgaca atcagcaaag gtcaaattgc gcacaatgat gttattgcac tcggcgattt
     4921 ttatgccgaa gccattcagc ccggcgtcag aatccaatcc gactatggag atattagcag
     4981 tgcgcttgat ggcaattttt gaagaatagc cactgagcgt tccggaaatg tggacaatta
     5041 gaggtgccgt acttttattt tcgcgcgcgt gcatcaattc gtaaaagtgc tcagctgtag
     5101 taacgatgac gctgtcgccg ccttgcccac cggaagctcc gccgttcaag gtggcgaatc
     5161 cgaccggctc cgcagtcggc accagagaat agactccccg acctgatacg gtaagcgtta
     5221 cagtatcatt acctaaaata tttgtaataa ggtcggaaaa agtgcctgac tcagtcgggg
     5281 caaagcgaac atacacagtc gtatcgaagg aaaatgtcgt gtaggcaatc ggcaatgacg
     5341 gtacaaatcc cgtatcgggc gctaacgata aatcaaaatc cggtggagca ataatatgga
     5401 tcacgtcatt ttcaatcaag tgacggcctg tgagtgtgta gcttttgatc aatttctggg
     5461 aaataagcac actgccgaaa ttcaacacaa tcggtgatac gctaagttca ggtacgattg
     5521 ccgttgtaaa ggtaaatatg ccatattgag ttgtgaggta gttataagta tttccggttg
     5581 cgacgttgta ttcgaaaatc gccgcggtat aagtcgtatt gccggtcaag ccggtaacag
     5641 taacggtagt atcctcgccg tcataaacta ttctggtgcc atcgccttga tcaattgcct
     5701 cagtaaaaat tgacgaaacg cccctgaccg gcttgccatc ttccggtaac caggcgatct
     5761 cagttccggg gcgaattgtg agcacgcgtc gcgctccatc accgccgctg aaagataaag
     5821 tagccgtttt accggttacc gagtcaggtg atattaccga agctgcggtc ggttcatttg
     5881 ccagaaaggc tcccgatacg tttagggagg tggttgtagc gccgtcgctt ttatgttcga
     5941 ttgttccgct atactcgccg gatgagtcgg ctttcagatg aacggcgata gaaagaggtt
     6001 gaccgacgat atcgccatct aaaacgggca ggcggagcga gtcgccgcta ctaatccagg
     6061 tcgagccgtt gtccgctgag atctcgaagt tcgatggagg atcaactgtt acggaatcta
     6121 ttaggttttt accgcttagc aaataggtct gggctgatga gggaattgcg gcattttctt
     6181 ggataaatcc gtccagcgcc gccggtgaag ccagcatgta agtagatggt atagttgtac
     6241 ccgatataac caccgaatta gtgatgatgt acttgccggt tgttgaagtg gtgtaccacg
     6301 gataaatgcg gacatagaaa gtatcaccgt aagcaacttc gcagtttaag gtagccgtga
     6361 tatttggggc tgatggtacc gatgaactta aggtgtaggt cgaaccgatt tgggtcttgg
     6421 tcacaaagag cgggtcggtc gcgtaataca cgttagcgcg catattactt ccagaattga
     6481 catacagttt catttcaatg gaatttacgt tgaaaatata accttcgacc ggtgttactg
     6541 cgaattgcat aaaacggttg atattctcgc tggtttcctc cggccaggaa ccggctgttc
     6601 cactcggact actgcgttgc acaccgcttt cgtacttgac ctgcatattg tttaaagtct
     6661 gaggagcggc gcaaaggttt ccggtaactg aggcattttg attggcggtt agtggccagg
     6721 tgctcgtagc actggtttgg gcgagcagtt gaccgatgat gagccagttt aaaatcattg
     6781 aagtaaaaaa cggt
//
LOCUS       DAOMJU010000100         6794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_11888_length_6794_cov_18.557056, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000100 DAOMJU010000000
VERSION     DAOMJU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11888_length_6794_cov_18.557056"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..750)
                     /locus_tag="PLO58_08470"
     CDS             complement(22..750)
                     /locus_tag="PLO58_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQO74863.1"
                     /translation="MKALILAAGLGKRLRPLTNQKPKALIEVGGRPLLQIAIEYLQMY
                     GFDEIIINVHHHADQIIKYLEVNKYLGQNIIISDETDQLFDTGGALKKAAWFFEDSQP
                     FLVYNVDILCNLDLRQFYQHHRESGALVTLAVRQRSSERYLLFDEANVLCGWQNLKTS
                     EIIQTRPSDSPLCQLAFSGISVLSPEILRHLPHKNIFSLIDLYLRLAPEFIIKGFQHN
                     ESRWLDVGNPENLAKAAEFFTITS"
     gene            complement(845..2311)
                     /locus_tag="PLO58_08475"
     CDS             complement(845..2311)
                     /locus_tag="PLO58_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQO74864.1"
                     /translation="MNPEQEKSLIHLFENWADEKAQNIYQLPSSGSYREYYRIQGNKQ
                     TALGVFNRDHKETRAFIEFARHFRSIGLNVPRIYADNAKNNIYLIEDLGDLTLYSYLV
                     NNYKNGIFPDKLIDMYKKVIAYLPRFQILADKGLNYSFCYPRSKFDKRSMLWDLNYFK
                     YYFLKLAKVSFNEEELENDFQTFCDFLLQADCNYFLYRDFQSRNILLVDGEPYFIDFQ
                     GGRKGALQYDIASLLYDAKADIPQHLRDEFLDEYIRQVSRYLTIDEKSFREFYWGYVL
                     IRIMQAMGSYGFRGFYEKKEHFLKSIPYAVANLRFILNNVTLPIEIPALWQALTQIVE
                     SSKYSNNSAVTSVTSSVLKVRINSFSYRNGIPADPSGNGGGFVFDCRGIPNPGRYEKY
                     KSLNGHHPEVIKFLKEETDIDEFLDYVYAIVDKTISNYIERQFTNLMVSFGCTGGQHR
                     SVYCAEELARHLRDKFRIQIELNHTALENTSGLSEIHS"
     gene            2459..3499
                     /gene="ltaE"
                     /locus_tag="PLO58_08480"
     CDS             2459..3499
                     /gene="ltaE"
                     /locus_tag="PLO58_08480"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HQO74865.1"
                     /translation="MSEIRIDLRSDTVTKPSPEMRAAMASAEVGDDVYGEDPTVNRLQ
                     ERVAALLGKEAALFVPSGTMSNQIAIKCLTQPGDEIICEYESHIYTYEASGPAFNSLV
                     MPRPVKGHYGVMAIEDVVRAIRPDNIQTGQTSLIEVENTHNRAGGTIYPLAEIEKLAE
                     IANEHRIPMHLDGARLMNAAVSTGIAPAEYAKYFDSVSLCLSKGLGAPIGSVLSGSRN
                     FIAKALRVRKIFGGAMRQVGILAAAGLYALDHNVQRLKIDHENAWLLAQGLSEIEGLY
                     IDKQQVQTNMVMIFARSPRWTTEKIVQQLLEKGIGMNAVDQERIRAVTHLDVSRDQIL
                     ETIEIFKKIMEA"
     gene            3503..4843
                     /gene="trkA"
                     /locus_tag="PLO58_08485"
     CDS             3503..4843
                     /gene="trkA"
                     /locus_tag="PLO58_08485"
                     /inference="COORDINATES: protein motif:HMM:NF007038.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HQO74866.1"
                     /translation="MKIIIIGAGEVGYYLAKRLISEDHNVTIIEMDSERHRRAAETLD
                     AIVIHDSGSSPRVLENAGVKSADILLAVTGSDDTNILSCIIAKKLGVARCLARVHSEE
                     FSFSKSAITGQDIGIDLLIHPERTAAEATIRLVEQSSASQLVTFEDDRLQIISLLVKE
                     DSPVVNLTLKEVATQHDQFSFLCLAIHREGKTIIPRGKNVYRSGDTAYFIAKEEDIPR
                     LTALLGYPKTEQQHIMILGAGRLGRMVAAVLSREMNVKLIEQDRDIAEEVAVNLPDTL
                     VLHGDGTDIDFLSSERIEEMDCFVAVSGSEKTNLLSGLLAKHLGVKRVIIHITTNEYI
                     PLMDKIGIDAVVSKNVETVNAIMRYVRRGNVIAVSLFEDIKAEAIELIPHVGSDITKK
                     PIKDLELPEDMIIGALIRQKEIIIPRGDTQIQPQDKVVVFLKPNLIQKVEKYFN"
     gene            4849..5592
                     /locus_tag="PLO58_08490"
     CDS             4849..5592
                     /locus_tag="PLO58_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQO74867.1"
                     /translation="MKPPVILIIKTGSTYSDLRNALGDFDIWIKNAAGDFNCNWKVKP
                     VADVEPDKIPNYQGVIITGSHSSLTQFYAFYGGMERVVQQIIDHRIHTLAICFGHQLV
                     HKIMGGTIDANPLGTELGVVTINLTLQGLVDPLFAGQSGSRIQVYASHSDIVIKPAPS
                     FVQLAWNKLALFQATRYDDFIYTTQFHPEYTKEIMDWYLKKNLALLTKQHISNPLHFA
                     TADEILKRNKSLPKSKLIIQNFLNLINSK"
     gene            5593..6327
                     /locus_tag="PLO58_08495"
     CDS             5593..6327
                     /locus_tag="PLO58_08495"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HQO74868.1"
                     /translation="MQQIINHPWNVSIKEARAIQLQLAKEVILQQFTGNIAKIWAVDV
                     SYGRFDSIGYGILGWFVTADDSSGGQFDCQMIKTFTTISSVAFPYVPGYLSFREIPVL
                     LPLFQELPEKPDLVLVDGAGIAHPRGIGLAAHLGVIFNVPTIGCAKSRLFGNYTEPAN
                     ESGAFSQLTNDDNIIGFVLRTKKNTKPLFISPGHLTDPQSSLEIIKRFCGKYRIPEPL
                     RRVDFISKELRRNPDQFQSNIIYCNQ"
     gene            6347..6790
                     /locus_tag="PLO58_08500"
     CDS             6347..6790
                     /locus_tag="PLO58_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HQO74869.1"
                     /translation="MKKLYRSRKHRIIGGVCGGIAEYFEIDPVLVRLIWVILVIFGGM
                     GILAYIIAWIIIPNRDESNSTVEQPDQPSEPQSSTEVATNTSNKNLFWGIVLIVIGLL
                     IIANQFWWPFDFIRTLVRGIFKYFIPAVLIVLGIFIILQRKETKK"
BASE COUNT         1999 a         1305 c         1544 g         1946 t
ORIGIN      
        1 tgacagcctg gaaaggctgt cctacgaggt tatagtgaaa aattctgcag ctttcgccag
       61 attttcggga ttaccgacgt ccagccagcg ggattcatta tgctgaaaac ccttaatgat
      121 gaattccggc gccagacgaa gatagaggtc tatcagcgaa aaaatatttt tgtggggtaa
      181 atggcggaga atttccggac tgagcaccga gattccgctg aaagctaatt gacacagagg
      241 tgaatcggag ggacgcgtct ggataatttc gctggttttg agattttgcc aaccacatag
      301 gacattagct tcatcgaaaa gtaaataacg ttccgacgaa cgctggcgaa ccgccaaagt
      361 gactaacgcg cccgattcgc ggtgatgctg ataaaattgc ctcaggtcga gattgcacaa
      421 aatatcaaca ttataaacca gaaaaggttg gctatcctcg aaaaaccagg cggctttttt
      481 taaagcgcct cccgtgtcga aaagttgatc agtttcatct gaaattataa tattttgccc
      541 gagatattta ttgacctcaa gatattttat gatttgatcg gcatgatgat gaacattgat
      601 aataatttca tcaaagccat acatttgtaa atattcgatt gctatttgca ataggggacg
      661 gccgccaact tcgatcaatg ctttaggttt ttggttggtg agcggtcgga gccgtttacc
      721 gagtccagcg gcaagtataa gagctttcat attggtgatc gcaaattgtt aacggacggt
      781 aattttaggg caggcttgaa actaaaaacc ataaaatgag aaataggagt tttacctaaa
      841 atgttcatga atgaatttct gaaagtcctg atgtattttc cagcgcggtg tgatttagtt
      901 cgatctggat tcggaattta tcacgtaaat gacgagcgag ttcttcggca caataaaccg
      961 agcgatgctg accgccagta cagccgaaac ttaccatcag attagtaaat tgccgttcga
     1021 tgtaattact tatagtttta tcaacaatgg catagacgta atccaggaat tcgtcaatgt
     1081 cggtctcttc ttttaagaat ttaatgactt ctggatgatg accattgaga gatttgtatt
     1141 tttcgtagcg gccgggattt ggaatgccac gacaatcgaa gacaaaacca ccaccgtttc
     1201 cgctcggatc ggctgggatg ccgttccggt aagaaaagct atttatccgg acttttaaga
     1261 cgcttgaagt gactgaagtt accgccgaat tgttagaata tttcgatgat tctacaatct
     1321 gagttagtgc ttgccaaagg gctgggattt caattggcag agtcacgtta ttcaaaataa
     1381 aacgcagatt ggctacggca taggggatac ttttcagaaa atgttctttt ttctcgtaga
     1441 atccgcgaaa tccgtaggaa cccatcgcct gcataatcct gatcaaaaca tacccccagt
     1501 agaattcacg gaacgatttt tcgtcaatcg taaggtagcg actgacctgc cgaatatatt
     1561 catccagaaa ttcatcacgc aaatgttggg ggatatcggc tttagcatca taaagtagag
     1621 aggcaatgtc atactgtaag gcgcctttcc taccgccttg aaaatcaatg aaatatggtt
     1681 cgccatcaac aagcaatata ttgcgcgact gaaaatcgcg atagaggaaa taattgcaat
     1741 cagcctgtag aagaaagtcg caaaaggtct ggaaatcgtt ttcaagttcc tcttcattaa
     1801 agctgacctt tgccagtttc agaaaataat atttgaaata attcaaatcc cataacattg
     1861 agcgcttatc gaatttcgaa cgcggataac agaaggagta attcaaaccc ttgtcagcca
     1921 gaatttgaaa acgcggcaaa tacgcgatga cttttttata catatcaatc agtttatcgg
     1981 ggaaaatgcc atttttataa ttattaacaa ggtaggaata taaggtcaaa tcgccaaggt
     2041 cttcgataag gtagatgtta tttttcgcat tatcggcata aattctcggt acatttaatc
     2101 caattgagcg gaaatggcgc gcaaattcga tgaacgcccg agtttcctta tgatcgcgat
     2161 tgaaaactcc caaggcggtt tgcttgtttc cttgtatccg atagtattcg cggtatgaac
     2221 cggaactggg taattgataa atgttttggg ctttttcgtc agcccagttc tcgaaaagat
     2281 ggattagtga tttttcttgt tcggggttca tagctataat ttcggatgtt cgtagtcatt
     2341 atggaaggat ttttctggat tgggaaaatt atttcccaaa ccatcatcct gaatttgaaa
     2401 aaccgctaat aattaattaa tttgaaacgg taaaatttat aaaggaaatg ttaacggtat
     2461 gagtgaaatt cgcatagatt tacggagcga tacagttacc aaaccttctc ctgaaatgcg
     2521 ggcggcgatg gcgagcgccg aagttggtga cgatgtttat ggcgaagacc cgacggtaaa
     2581 tcgtctgcag gagagggtag cggctcttct gggcaaggaa gccgctttat ttgtcccgag
     2641 tgggacaatg tcgaaccaga ttgcgattaa atgtcttact caaccgggcg atgaaattat
     2701 ctgcgagtat gaaagtcaca tttacaccta tgaagctagc ggtccagcat tcaattcgtt
     2761 ggtaatgccg cgtccggtga agggacatta tggagttatg gcaatcgagg atgttgtcag
     2821 agctatacga ccagacaata ttcagaccgg gcagacgagt ttaatcgagg tcgaaaatac
     2881 tcataatcgt gctggtggta cgatttatcc attggcagaa attgagaaac tggctgaaat
     2941 tgccaatgaa catcgtattc cgatgcactt agacggtgcg cgcctgatga atgcggcggt
     3001 gagcaccggc attgcccccg ccgaatatgc taaatatttc gattcggtga gtctatgttt
     3061 gtcaaaaggt ctgggcgcgc cgatcggatc ggttttaagt ggttcgcgga actttattgc
     3121 taaagccctg cgagttagga aaatttttgg tggagcaatg cgacaagttg ggattttggc
     3181 cgcggctggt ttatatgccc tcgatcataa tgttcagcgc ctgaaaattg accacgagaa
     3241 tgcctggcta ttggcacaag gactgtcaga aatcgaggga ctgtatatcg ataaacaaca
     3301 ggtgcaaacc aatatggtga tgatttttgc ccggagtccg cgctggacga ctgaaaaaat
     3361 tgttcagcaa ttattagaaa aaggtattgg gatgaatgcc gttgatcagg aaagaattcg
     3421 cgccgtaact catctcgatg ttagtcgtga tcaaatactt gaaacaattg aaatatttaa
     3481 gaaaattatg gaagcttaag caatgaaaat aattattatt ggcgccggtg aagttggtta
     3541 ttatttagcc aagcgtctta ttagcgaaga tcataacgta actataatcg aaatggattc
     3601 ggaacgtcat cgtcgagcgg cagaaacact tgatgccatt gttattcacg atagtggttc
     3661 cagtccgcga gttttggaga atgctggagt aaaatctgcc gatattcttt tagcggttac
     3721 gggaagtgac gacaccaata ttctatcctg tataatagcc aaaaagctgg gagtagcccg
     3781 ttgtttagcg cgtgttcata gtgaagaatt cagtttttca aagtcggcta ttaccggtca
     3841 ggatatcggt attgacttat tgattcatcc ggaacggacg gcggccgagg ctactatccg
     3901 gctggtggag cagtcctcgg catctcaatt ggtaactttt gaagatgacc gtttgcagat
     3961 tatttcattg ttagtaaaag aagatagtcc ggtagttaac ttaactttga aagaagttgc
     4021 tactcagcat gaccagtttt catttttatg tttggcgatt catcgggaag gtaagaccat
     4081 cataccgcgg ggaaaaaatg tttacagaag tggtgatacg gcttacttta ttgctaaaga
     4141 agaagatatt ccgcgtttaa cggcactgct cggttatccc aagaccgaac aacagcatat
     4201 tatgattctg ggcgctggtc ggctcgggag aatggtcgca gctgttttat cgcgtgaaat
     4261 gaatgtcaag ctgattgagc aagatcgtga tatagctgaa gaagtagccg ttaatttacc
     4321 tgatacatta gttttgcatg gtgatggtac cgacattgat tttcttagtt cagaaagaat
     4381 agaagaaatg gattgttttg tagcagtttc tggtagtgaa aagaccaatc ttctttccgg
     4441 tttgcttgcc aagcatcttg gagttaaacg cgtcattatt cacattacta ccaatgaata
     4501 tattcctcta atggacaaga taggcatcga tgcagttgtc agtaaaaatg ttgagacagt
     4561 taatgcgata atgcgttatg tacggcgggg taatgtcatc gccgtctcat tattcgagga
     4621 tatcaaagcc gaagccatag aattaattcc acacgtcgga agcgacatca ctaaaaaacc
     4681 aattaaagac cttgaattgc cggaagatat gataatcgga gcgctgattc gccagaagga
     4741 aataatcata cctagaggcg atacccagat tcagccccag gataaagtag tggtctttct
     4801 aaaacctaat ttgattcaaa aagtcgaaaa atatttcaat taaatcgaat gaaaccgccc
     4861 gttatcctta taattaaaac cggttcgact tatagcgatt taagaaatgc gttaggtgat
     4921 ttcgatattt ggatcaaaaa tgccgctgga gattttaatt gcaactggaa agtgaagccg
     4981 gtcgccgacg tcgaaccgga taaaatccca aactatcaag gcgtaatcat aaccggttcc
     5041 cattcttcgc ttactcagtt ttacgcgttt tacggtggaa tggaacgtgt ggtccaacaa
     5101 ataattgacc accgcattca tacacttgca atctgttttg gccaccagct ggttcataaa
     5161 attatgggcg gtacgattga cgcaaatcct ttgggaaccg aattaggtgt cgttaccata
     5221 aatcttactc tacaaggctt agtagatccg ctatttgccg ggcagagcgg tagtcggatt
     5281 caggtttacg cttcccattc tgacattgtt ataaaaccag ccccgagttt tgtccaatta
     5341 gcctggaata aattggcgct atttcaggcg acgcgctatg acgattttat ttatactacc
     5401 caatttcatc cggaatatac taaggaaata atggattggt atttgaaaaa gaacctggcg
     5461 ctattaacaa aacaacatat ttctaatccc ctccacttcg caacggctga tgaaattctc
     5521 aagcggaaca aatcattacc aaagtccaaa ctgattatcc agaattttct taatttgatt
     5581 aacagtaaat aaatgcaaca aattataaat catccctgga acgtatcaat caaagaagcc
     5641 agagctattc agttacaact agctaaggag gtgattctcc aacaattcac cggtaatata
     5701 gccaaaatat gggcagttga cgtctcttat ggacgctttg atagcatcgg atatgggatc
     5761 ttgggctggt ttgtcactgc tgatgattct tctggcgggc aatttgattg ccaaatgatc
     5821 aagactttta cgacgattag ttcagtcgct tttccgtatg ttcccggata tctgagtttt
     5881 cgcgaaattc cggtgctttt acctctgttt caggaattgc cggaaaaacc tgatttggtg
     5941 ttggtagatg gggctggaat tgctcaccca cgcggaatcg gattagccgc ccatctggga
     6001 gtaattttca acgtgcctac aatcggttgt gccaagtcgc ggctatttgg taattatacc
     6061 gaaccggcta acgaaagcgg ggcattttct cagttgacta atgatgataa cattatcgga
     6121 tttgtcctgc gaacgaaaaa gaataccaag ccgcttttta tttcacccgg tcacctgacc
     6181 gatccccaat catcgctcga aattatcaaa cgattttgtg ggaaatatcg gattcccgaa
     6241 ccgcttcgga gggtagattt catctcaaaa gaattacgtc ggaatcccga tcaattccaa
     6301 agcaatataa tttactgtaa tcaataaatt agaggtgagg tagaaaatga aaaaacttta
     6361 tcgtagtcgc aagcaccgaa ttattggcgg agtttgtggt ggcattgctg agtattttga
     6421 aattgacccg gttttagtac gactaatctg ggtaattctg gttatttttg gcggaatggg
     6481 catattggct tatatcattg cctggattat tattccaaat cgagatgaat cgaactcgac
     6541 agtcgaacag ccggatcaac cttccgaacc acagtcatca actgaagtag caactaacac
     6601 gtcgaataag aaccttttct ggggaatcgt gctgattgtt ataggtctgc taatcattgc
     6661 caatcaattc tggtggccat ttgattttat tcggactctg gtgagaggta tttttaagta
     6721 ttttattccg gctgttttaa ttgtgttagg tattttcatc attttgcaga gaaaagaaac
     6781 aaaaaagtaa ctaa
//
LOCUS       DAOMJU010000101         6639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_12271_length_6639_cov_18.546932, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000101 DAOMJU010000000
VERSION     DAOMJU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12271_length_6639_cov_18.546932"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLO58_08505"
     CDS             <1..578
                     /locus_tag="PLO58_08505"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO74870.1"
                     /translation="GLGYAKRLTEWFSVGGQIRLVKQDLGQSVIPKISTIPDTTEYVV
                     KNTLTPLAFDFGTQFKTGFKSLVFGMSIRNFSTDIKYVEEPFQLPLVFNLGISMDLFD
                     LISQINQNQSLYMSIDASHYRSRAEQVKIGLDWQLLKVLSLRCGYITNEDESGLTFGI
                     GVSKFGFTFDYAYTPYGVFNQVQRLTVRMSF"
     gene            601..1791
                     /locus_tag="PLO58_08510"
     CDS             601..1791
                     /locus_tag="PLO58_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74871.1"
                     /translation="MKSNRFLIISALCLAVMKCEYDVTQPQWYQDYTEPATPSITNIE
                     PDSAVAGCNYINIIGENFSGTPTVYFDNVTAEVISNLGNSIQVRRPNLASDGCLVKVA
                     CDSALVVAQSTEPYKITQVVQKYGNFVENKELNAVAVDSAENVYVIDYTNIIYKVTPA
                     GDKTTMLDKAKRTTYDATIGPDGKLYLLGNNRSIDVLDPQSGKVAEWIKLKAGKVVKF
                     GDFDKNGYLYTGGRSTGLVVVAPDLTPNYTSLYEKDEIKGIRVFGNYVYVSLVCTTPD
                     LQNPALAIWRNPIDNSGNPGEKELVLDLTAYKNYSADALNSFSLSSDGKLFLGTNAQT
                     PLLAIDLNTTSADYFYLGIIAPYCKSFSWGWGNYLYMICGDTALGVEWTLYRIDMGVT
                     SARE"
     gene            complement(1884..4673)
                     /locus_tag="PLO58_08515"
     CDS             complement(1884..4673)
                     /locus_tag="PLO58_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQO74872.1"
                     /translation="MKSQITYSLIILALAVSQCGQSGKVKPIQNGVDLKTRDLNVRVQ
                     FYADDIARVIKWRPESSAEKLSLMVIRDSLPDLSITVEENDNDVILKSERMTIRLVKK
                     DGHLEYFTTSGTSILKENKKAIFTPVTLPYEKAFNIQQNFKLTPDEGLYGLGQHQDGY
                     MNYRGRTVKLVQTNTDAVTPFLISTRGWGILWDNYSKTIFQDTAETMSLWSEVGDNLD
                     YYLIYGGTMDSVISGYRYLTGKAPLYGKWAYGYWQSKEHYKNRAELLSVVSEYRRRRI
                     PIDNIIQDWNYWDGNENWSQMFFDPKKYPEPEEMIRQIHNQNFHIMISIWPGLGPNTA
                     IYQEMEQNGFLYNTVGWANFKYFDAFNPAANDLYWKYIKNGLISVGIDALWIDSTEPD
                     IVNATTKESQEYEMKKVGRNYLGSWARYLNAYSLVMMDKLYQNQRQDSDRKRVYMLTR
                     SVFAGQQRTGATTWSGDIGASWEIYQNQISAGLNFCMAGIPYWTFDIGGFVIGAYGGV
                     FINGGQDPAYQELYTRMFQFGTFCPIFRAHGSETPREIWCFGEFSPHLIKFDHLRYRL
                     MPYIYSLAWRVTNDDYTMMRGLPMDFPNDPETYSLGDQYMFGPAIMVCPVTEYMLHRP
                     PGKSILISGDHFKTNDGKAGLKVQYFRDPDFRILSHEQIEPDINHLWYTGRPDYMTDS
                     AFSVRWEGKLIPSQTGLHQFHLKSYDAKRIFINGKELPIVYTSVEQYTDLINLKAGQG
                     YDFRLEMQNRSTGAAKMQLNWKTPAIFAQEKIVETRPQTRNVYLPAGTQWIDFWTGTT
                     YSGGQTIVADAPIDKIPLLIKAGSIVPMGPFLQYSTEKPADPLEVRIYRGADGSFTLY
                     EDENDNYNYEKGAYSTINFYWDDTKNELTIGERNGSFPGMLMERQFQIVLVSPNHGIG
                     IEITPRPDKIIKYLG"
     assembly_gap    5023..5122
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5191..6261
                     /gene="ispG"
                     /locus_tag="PLO58_08520"
                     /gene_synonym="gcpE"
     CDS             5191..6261
                     /gene="ispG"
                     /locus_tag="PLO58_08520"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQO74873.1"
                     /translation="MNNFKRKITRTVYVGNVPVGGGFPIPVQSMTTAKTHDLKAILPQ
                     IEKLEAAGCQIIRVTVPDEQALKALPEIKKRMTVPLVADIHFDYRLALGAIDAGADKI
                     RINPGNIGGAERVKAVLGKARAAHIPIRIGVNSGSLEKDLRAKYGGATPEAMLESARR
                     HLEICEENDFRDVIVALKSSEASLMIAANRLFSEKYDYPLHLGVTEAGPVWSGTIKSA
                     IGIGTLLAEGIGDTLRVSLTANPVEEVRAGFEILKALNLTKSGIRLVSCPTCGRTSVN
                     LIEIVNEVESKIRDLPQNLTVAVMGCEVNGPGEARSADLGIAFGKGVALLFKHGQVVA
                     RLKPEEAVARLLAEIENWSPTQ"
     gene            6273..>6639
                     /locus_tag="PLO58_08525"
     CDS             6273..>6639
                     /locus_tag="PLO58_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74874.1"
                     /translation="MHKITLWLGCILLFGNLFAQEPAVPEVIIQESDTSETALPDSAT
                     EAMPQVEIPTVPLTPAQLPSETLTEEPAQPEPAPEELKPPEEVLPVLVASQKAKMKPS
                     EITHFGQMIYWKSLTPSEKK"
BASE COUNT         1831 a         1410 c         1453 g         1845 t
ORIGIN      
        1 taggactggg ttatgcgaag cgcttgacgg agtggttcag tgtgggcggt caaatccgcc
       61 tggtcaaaca ggatttaggc caaagtgtga taccgaaaat ttctaccatt cccgacacta
      121 ccgagtatgt agtcaaaaac acactgactc cgctggcttt tgatttcggg acgcagttca
      181 aaaccggttt taaaagcttg gtcttcggga tgtccattag aaatttctcc accgacatta
      241 aatatgtcga agaaccgttt caactacctt tggtttttaa tctgggaatt tcgatggatt
      301 tattcgacct aataagtcaa attaaccaaa atcaatcact gtatatgagc attgatgcca
      361 gtcattatcg ctcccgcgct gagcaagtga aaatcggtct agattggcaa ctattgaaag
      421 tgttgtcatt acggtgcggt tatatcacta acgaagatga gagcggtttg acttttggaa
      481 tcggcgtatc caaattcggt tttacgtttg actatgccta tacgccgtat ggtgttttca
      541 atcaggtaca gcgtttgaca gtacgaatgt cattttgagc gatagaagga gaaaagtatc
      601 atgaaatcta atcgcttttt aataatctca gccctctgtt tggcagtaat gaaatgcgaa
      661 tatgatgtta ctcaacctca atggtatcag gattataccg aaccggcaac tccatctatt
      721 actaatattg agccggacag tgctgtagcc ggttgcaatt atattaacat tattggtgaa
      781 aatttcagcg ggacgccaac ggtctatttt gataatgtaa cagcggaagt aatctccaat
      841 ttgggcaatt ctatccaggt tcgtcgtccc aatctggcaa gtgatggttg tctggttaag
      901 gtggcttgcg atagcgctct tgtggtcgcg caaagcactg agccttataa aattactcag
      961 gttgtccaaa aatatggtaa tttcgtcgaa aataaagaat taaatgccgt agcggttgat
     1021 agtgctgaaa atgtatatgt aattgattac accaatatta tttacaaagt aactccagct
     1081 ggtgacaaaa ccaccatgct tgataaggct aaacgcacaa cctatgatgc taccattgga
     1141 ccagatggaa aattgtatct tttgggtaac aaccgctcaa ttgacgtttt agatccacaa
     1201 agcggcaagg ttgctgagtg gattaagctt aaggcaggca aggtggtaaa atttggcgat
     1261 tttgataaga atggttattt atataccgga ggtcggagca cgggactggt tgtcgtcgct
     1321 ccagatctta cgccgaatta taccagtttg tatgaaaaag atgaaatcaa aggtattcgc
     1381 gtgtttggta attatgtgta tgtaagcctg gtttgtacca cccccgactt gcaaaatccg
     1441 gctctggcaa tttggcgtaa tccaattgat aatagtggca atccaggaga aaaagaatta
     1501 gtgcttgatt tgacggctta taaaaactat tctgctgatg ctttaaatag cttttcgctt
     1561 tcatctgacg gcaaattatt tctgggcact aacgcgcaga ctcctcttct tgccattgat
     1621 cttaacacca cgagcgccga ttatttttat ctgggtataa tagctcccta ttgtaaatct
     1681 tttagctggg ggtggggaaa ttacctatat atgatttgtg gcgatacggc tcttggagta
     1741 gagtggacac tctatcgcat tgatatggga gtcaccagcg ctcgggaata atctgtctgt
     1801 cactcaacta aagaaaaacg gccggctttt gactggccgt tttgttttta tgaagaataa
     1861 aattagagcc tgattgtctg cgcttagccc aaatacttaa tgattttatc cggtcgcggg
     1921 gtaatttcga ttccgatgcc atgattgggt gaaacaagca ctatctggaa ttgacgttcc
     1981 atcaacatac ctggaaaaga accgttacgt tctccaatcg tcaactcatt tttagtatca
     2041 tcccaataaa aattaatagt cgaataggca cctttttcat aattgtaatt atcgttttca
     2101 tcctcataaa gagtaaaact gccatcggcg ccccgataaa ttcgcacttc gagcgggtcg
     2161 gctggctttt cggtggaata ttggaggaaa ggtcccatcg gtacaattga accggctttg
     2221 attaataagg gaattttgtc aattggagca tcggcgacaa tggtctgacc gcccgaatag
     2281 gtcgttccag tccagaaatc aatccactgc gttccggcag gtaaataaac attgcgggtt
     2341 tggggtcgcg tctcgactat tttctcttgg gcaaagattg cgggggtttt ccaatttaac
     2401 tgcatcttgg cggctccggt cgaacgattt tgcatttcga ggcgaaaatc atatccctgt
     2461 ccagccttca ggttgatcag gtcggtatat tgctcgacac tggtataaac tatcggcaac
     2521 tccttgccat tgataaaaat gcgtttagcg tcgtagcttt ttaagtgaaa ttgatgcagg
     2581 ccggtctgac tggggatcaa tttaccttcc cagcgtactg aaaaagcgga atcggtcata
     2641 taatccggtc ggccggtgta ccagagatga ttaatatcgg gctcaatctg ttcgtgagat
     2701 aggattctaa aatctggatc acgaaaatat tgaactttta agcctgcttt tccatcatta
     2761 gtcttaaaat ggtcaccgct gattaaaatg ctttttccag gtggacgatg caacatatac
     2821 tccgttaccg ggcagaccat aatagccgga ccgaacatat attggtcgcc gagagagtaa
     2881 gtctcaggat cgttcgggaa atccatcggc aaaccgcgca tcatagtgta atcatcatta
     2941 gtcacccgcc aggctaagga ataaatataa ggcataagcc ggtagcgaag atggtcaaat
     3001 tttattagat gcgggctgaa ttcaccaaag caccagattt cccgtggcgt ttcactcccg
     3061 tgtgcccgga aaattggaca gaaagttcca aattgaaaca tacgagtata aagttcttga
     3121 taagccggat cctgaccgcc attaatgaat actccaccat aagcgccaat gacgaatcca
     3181 ccgatgtcaa aagtccaata aggaatacca gccatacaaa aattcagacc ggcgctaatt
     3241 tgattttgat aaatctccca gctggcgcca atgtcgcctg accaggttgt ggcaccggtt
     3301 ctctgttgac cagcaaagac ggaccgggtc agcatatata ctcttttgcg gtcggaatcc
     3361 tgacgctgat tctggtagag tttatccatc attaccaaag aatatgcgtt cagataacgc
     3421 gcccaactgc caaggtaatt ccgacctacc tttttcattt cgtattcttg ggattctttg
     3481 gtggtggcat tgacgatatc cggttcagtg gaatcaatcc agagcgcatc gatgccgacc
     3541 gagattaaac cattttttat atatttccag taaagatcat tagcggctgg attgaaagcg
     3601 tcgaaatatt tgaaatttgc ccatccgacg gtattgtaga ggaacccgtt ttgctccatt
     3661 tcttgataaa ttgcggtatt cggtccaagt cccggccaga ttgaaatcat tatatgaaaa
     3721 ttttgattgt ggatttgccg gatcatttcc tcaggctcgg gatatttttt cggatcaaag
     3781 aacatctggc tccaattttc attgccgtcc caataattcc aatcttggat aatattatca
     3841 atcggtatgc gacgccgacg atattcactc acaacgctta ataattcagc gcggttttta
     3901 taatgctcct tgctttgcca ataaccgtaa gcccattttc catacaaagg agcctttcca
     3961 gtgagataac gatacccact aatgacggaa tccattgttc cgccataaat taagtaataa
     4021 tcgagattgt caccgacttc cgaccaaagt gacatcgtct cagccgtatc ctgaaaaata
     4081 gtcttggagt aattatccca cagaatgccc catccgcgag tagaaatcaa aaaaggagtt
     4141 accgcgtccg tattggtttg gacgagtttg acagttcgcc cgcggtaatt catatatccg
     4201 tcttgatgtt gaccaagccc gtagagccct tcgtctggtg ttaatttgaa attttgctga
     4261 atgttgaaag ctttttcata cggaagcgtt accggagtaa aaatggcttt tttattttct
     4321 tttaaaatac ttgtgccgct ggtcgtaaaa tattccaaat ggccatcttt cttaaccagg
     4381 cgaattgtca ttcgctccga ttttaggatt acgtcgttgt cattttcctc aaccgtaata
     4441 gataaatccg gcaaactatc tctaatgacc attaaactta gcttttcagc tgaagattcc
     4501 ggccgccact taatgactcg ggcgatgtcg tcagcgtaaa attggacacg tacattcaaa
     4561 tcacgagttt ttaaatcaac cccattctga atgggtttaa cttttccgga ctgaccgcat
     4621 tgtgataccg ccagcgctaa aattattaac gaataagtaa tctgtgattt catttattcc
     4681 ctcatttttt aggtttgtta aaagaccgct ggctataatt tttttaataa ctatcggaac
     4741 tgattcacat ttgaccggac ttgatggcgg ctctgcttca aaaaccttcg taatatcaat
     4801 ggaattatat tagtcaatct tcactatgaa aacaagcttt tgaatggtaa aaaagtagca
     4861 gattaaaccg atggatccta aaattatata attggacact gaggcgcaca gaggtaagcc
     4921 ggagagcacg gagcattttt aagtactttt aagttctatt aagttttttt aacattttta
     4981 agtgccttta ggaatttaat agtacttaag cacacttatc agnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nncagcactt tcgagcattt gataaattag gcttgtctca
     5161 ttgcgggagt aatattaatt tgattcggcg atgaacaatt ttaaacgcaa gattactcgt
     5221 accgtatacg tcgggaatgt tcccgtcggc ggcggatttc caattccagt gcaatcaatg
     5281 acaaccgcta aaacacacga cttaaaggcg attctgccgc aaattgagaa actggaagcc
     5341 gccggttgtc agataatccg cgtcaccgtc cccgatgaac aagccctcaa agctttaccg
     5401 gaaataaaaa agcggatgac agtgccactg gtcgccgata ttcattttga ttatcgcctg
     5461 gcactgggcg caattgatgc tggtgccgat aaaatccgaa tcaatcccgg caatatcggc
     5521 ggcgcagaac gagtgaaagc cgttctaggg aaagctcggg cggcgcatat tcctattcgc
     5581 atcggcgtca attccggttc actggaaaaa gacttgcggg caaaatacgg gggagcgacg
     5641 cccgaagcca tgctggaaag cgcccgccgc catctggaaa tttgcgagga gaatgatttc
     5701 cgcgacgtaa tcgttgccct caaatccagt gaagccagtc tgatgatcgc cgctaatcgg
     5761 ctcttttccg agaaatatga ttatcccctt catcttggtg ttaccgaagc cggtccggtc
     5821 tggtcgggca caattaaatc cgccattggt attggaacgc ttttagcgga aggaattggc
     5881 gacactttgc gggtgtctct gacggcgaat ccagtcgaag aagtacgcgc cggttttgaa
     5941 atactaaaag ccctaaattt aaccaaaagt ggtatccgac tggtatcgtg tcccacctgc
     6001 ggccgtacct cggttaatct gattgaaata gtgaacgaag tcgagagtaa aatccgcgat
     6061 ctacctcaaa atttaaccgt tgccgttatg ggctgtgagg tcaatggccc cggtgaagcc
     6121 cggtcggctg atctgggaat tgctttcggc aaaggagtag ctctattatt taaacatggt
     6181 caggttgtcg cacgattgaa accggaagag gcggtagcgc gattactggc ggaaattgaa
     6241 aattggagcc cgacgcaata ggaggtccca ttatgcataa aatcacactc tggcttggat
     6301 gtattttatt atttggtaac ctgttcgctc aggaaccggc agtgccggaa gtgattattc
     6361 aagaaagtga tacatctgaa accgcactgc cggattcagc tactgaagca atgcctcagg
     6421 tcgaaattcc cacagtacca ttgaccccgg ctcaattgcc ttcagaaacg cttaccgagg
     6481 aaccagctca gccagaacca gctccggaag aactaaaacc accggaggaa gttctcccgg
     6541 ttctggttgc atcccaaaaa gccaaaatga aaccatctga aattacccac ttcgggcaaa
     6601 tgatttactg gaaatcgctg actccttccg agaaaaaag
//
LOCUS       DAOMJU010000102         6468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_12712_length_6468_cov_20.650398, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000102 DAOMJU010000000
VERSION     DAOMJU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12712_length_6468_cov_20.650398"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLO58_08530"
     CDS             complement(<1..326)
                     /locus_tag="PLO58_08530"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQO74875.1"
                     /translation="MILIWSESLFSKNEFKATEYFVNKIIITGNKQFSTRKLKKQINL
                     KEKTIWRKQTFTRRLLELDRLNLESFYIKQGYLHCTVIDSFSVHSNNLVDVFFKINEG
                     QMYILK"
     gene            complement(374..4291)
                     /locus_tag="PLO58_08535"
     CDS             complement(374..4291)
                     /locus_tag="PLO58_08535"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQO74876.1"
                     /translation="MKKIIKISIIVIVSLVLLIGLTGFLILKTDSWKTILVSSVNRQL
                     MQNYNLKIEPEGIGGNIFYHLQFDRLKLTNQNQTELVTMSGVNLSYNLKSLIGHHHIL
                     NKLSLDTIKLAYPANIDSLLASFPEKKDTLASTGNFKLANILMSKCLIDDSHHPGQLL
                     VSIDSLNGSLSLNADTFNLQLNSTDFYVTGINEYFRIKQTAIAFVNNSLEIKNGQISN
                     RGTTVELDGRISLNKPYASQLRINVTDINPAERLPALSQVFRQDDIFDLSGEIKTSDK
                     TIKLDLGFSGTWHNQLLTNGRITGLIDGKQVNLAEMTFTKGMEQVHGTVVGNLDSSLT
                     AELRFSGIDASSWGLSAINTDLQGNLHVQTFGSIPNIDQVKVKIDLQESKIAPLDFNL
                     IRGELVYKDGLLTVLDSLYCVLGETKLIVEGEADLNGNSIDARAHFSDLNAEMLAPML
                     KMDTLSGVLDGFVEATGILTAPDLRGWIHAKQFGLPNLKFEETMARFGIVDVRTRKFG
                     DIFVEATNCQTNLIPEKIPLTSLIIHINGDTTIVQTLKAIGENLDVEAKGYIVQFTDF
                     NLNSITILRSGNVLQNIDPFSFSLKKDTLNVDAVRLRLNEGILTLTGQVVRGELQSGR
                     LEFANLSIDPINTYLKGSQGVGGKLEGMVAYKKNLEGVNYVYKINLDDAKLISQNFEN
                     IQLQGQLDNHRILIENLAVKDNQNGLLTGHGFLTCNLNSKNTKSFLSAQDSIGLTLNF
                     NRFNLGSIDDYLPSNLNIGGIVDGQINIFHYLSNPKMNCDLIIDDPVFDKLAGEKLTI
                     KGVYQNERLSITEAMLVDQYGHTRGRGYLALAISIVPARFEVLKEAPLSFNFTTNSSS
                     PSFLTAYLDFLQEVQGDISLALGISGTLENPILSGNISVKGTTIYLDLLENPITGING
                     SAVIKDNILEIVSLTGHMKKTPSSGGVGGFGSRLKSVTWDVLFPPKSDIDQPNVAING
                     TIDFTRFSAPKYNINLKGDELYIRTLLAEQEGLVSGNITLTGQDTVIYAGEIEVEDFV
                     IRNEFTGTKKAVVPKQPSKIYSIMNFHLIIPGSLEIKNSQLEGELEGDIWITRNGNEP
                     FRFSGTLDILDGQFFAYGWEFEIVRGSINLDPVEFNPTLDIEAQVDLASYGMSDTTTS
                     SSIESEVVTVYLTGYLDNPNIEFQSTNYSQSDILLFLSRAQSIGSITNGQDQLSASAA
                     NVASMWFERQLERNISRISGLDDFELRTNGNLFSSQQPDQWSVMLGRKIAPNLYIKYE
                     RSFSLIEPYQQFGLEYRINRNISIAGTYDQDGYSINYRYKYRY"
     gene            complement(4470..6281)
                     /locus_tag="PLO58_08540"
     CDS             complement(4470..6281)
                     /locus_tag="PLO58_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQO74877.1"
                     /translation="MNPLVISHRFFENVKKYADKPALLSKVDGHYRPVLYRDLGEIVK
                     AVALALDSWGIRKGDRVAILSENREEWVMSDMANQSMGAISVPIYPTLISNQIAPLFK
                     HSEPRVLFVANGTLLKKAMVTGYQFERIVIYDPDPELPQAVAFAEMVETGRKLDAKNP
                     EAFSALLNAVEPDDIACINYTSGTTGAPKGALLTNKNFVKDVDNSVGIMKMVPEDCFL
                     SFLPLSHVLERMGGYYTPMLMGNTIGFAENIDTVVQNMAEVQPTILVSVPRLFEKIYA
                     TVMSQIESGSAIKKKVFFWALKAGKNRMHSYIDKTPLTFWQKRKIKIADKLVFNKLQK
                     TTGGKIQRFVSGGAPLSKEIAEFLFSAGLPVLEGYGLTETSPVITLNGPDKVRLGSVG
                     VAIPETEFKIAEDSEILVRGPQVMKGYYKDEAATREVLDEEGFLHTGDVGYFDEDGFL
                     FITDRKKNILITAGGKNVAPQPIEDAIKLSPYISEAVLFGDRRSYIVALVTLDQEAVR
                     KWAENQHLSIDNYADFVRRPELITLIANEIERQTAHFSRFEKVKKFRIIPEDFTIESG
                     ELTPSLKVKKKAIAERYADLIDEMYQEQSQEKAQDLT"
     gene            6361..6450
                     /locus_tag="PLO58_08545"
     tRNA            6361..6450
                     /locus_tag="PLO58_08545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6395..6397,aa:Ser,seq:gct)
BASE COUNT         1902 a         1382 c         1243 g         1941 t
ORIGIN      
        1 tctttcagga tatacatctg accttcattg atcttaaaaa aaacgtcaac gagattattg
       61 gagtgaactg aaaatgaatc tataaccgtg cagtgaagat aaccttgttt aatataaaat
      121 gattcgaggt taaggcggtc aagctccagc agtcgccgag taaaggtttg cttgcgccag
      181 atagtttttt ctttgagatt aatttgtttt tttagtttgc gagttgaaaa ttgtttattg
      241 cccgttatta taattttgtt gacaaaatat tcggttgctt taaattcatt tttagagaaa
      301 agcgactcac tccatattaa aatcaaaatg gttaaaatta aactgtatga ccgaaaattt
      361 ctgtttatga ttttcaatag cgatacttat aacggtaatt tatactatat ccatcctgat
      421 cataagtccc agcaatcgag atgttccggt taattcgata ctccagtccg aattgttgat
      481 agggttcaat tagagaaaaa gagcgttcat attttatata caaattggga gcgattttac
      541 gtcccaacat tacgctccat tggtcaggtt gctgactcga aaagagattg ccattggtac
      601 gcaattcaaa atcatccagt ccactaatcc ggctgatatt acgttccaat tgacgctcga
      661 accacatact ggcgacattt gcggcgctag ccgaaagttg atcctgtcca ttggttattg
      721 agccgatact ttgagcccga gataaaaaga gcagaatatc gctttgggaa taattagttg
      781 attgaaattc gatgttagga ttatctaaat agccggttaa atatactgtt accacttcac
      841 tctcgataga agaggatgtg gttgtgtctg acataccata ggaagctagg tcaacttggg
      901 cttcaatgtc cagagttgga ttaaattcaa ccggatcgag attgatcgag cctcttacta
      961 tttcaaattc ccagccataa gcgaaaaatt gtccgtcgag aatatcgagc gtccctgaaa
     1021 aacggaacgg ttcatttcca ttccgtgtaa tccagatgtc gccctcgagc tctccttcca
     1081 attgtgagtt tttaatttca agactgccgg gaatgatcaa atgaaaattc ataattgaat
     1141 atatttttga gggttgtttg ggcacaaccg cttttttcgt accagtaaat tcgttgcgga
     1201 taacgaaatc ttcaacttca atttctcccg cgtaaataac cgtgtcctga ccggtcaaag
     1261 taatatttcc gctaaccaag ccttcctgct ccgccagtaa agtacggatg tatagttcat
     1321 cgccttttag attaatatta tacttgggcg cagaaaaccg ggtaaagtca attgtaccat
     1381 taattgcgac attgggttgg tcaatatcac ttttaggtgg aaatagcaca tcccaggtta
     1441 cagattttag gcggcttcca aaaccaccta cgccgcccga tgaaggagtt tttttcatat
     1501 gaccagtcag agaaacaatt tcaagaatat tatcttttat caccgcacta ccgttaattc
     1561 cagtaatagg attttcgagc aggtcgagat aaatagtggt gcccttaacg gaaatatttc
     1621 cggataggat gggattttcc agtgtccccg aaatgcccag cgcgagactt atatcaccct
     1681 ggacttcctg cagaaaatca agataggcag ttagaaatga tggtgatgaa ctgttcgtag
     1741 tgaaattaaa actcagcggt gcttccttta aaacctcaaa acgggcaggc acaatactaa
     1801 ttgctagcgc taaataccct ctgccccgag tatgaccata ttgatctacc agcatagctt
     1861 cggtgattga aagacgctca ttctggtaaa cacctttaat agtgagcttc tccccggcca
     1921 gtttgtcaaa aaccggatcg tcaataatca aatcgcagtt cattttaggg ttactaagat
     1981 aatgaaagat atttatctgc ccatctacta ttccacctat atttaggttc gatggtagat
     2041 agtcatctat gcttcctaaa ttaaaacgat tgaaatttaa ggtcaaaccg attgaatcct
     2101 gggcactcaa aaaagacttt gtatttttag aatttagatt acacgtcaaa aagccgtgcc
     2161 cagtaagtag tccattttgg ttatctttaa cagctagatt ttcgattaga atccgatgat
     2221 tatccagctg accttgaagt tgtatatttt caaaattttg actgattaac ttagcatcat
     2281 ctaaattaat tttatagaca taatttaccc cttccaggtt ctttttataa gccaccatgc
     2341 cttcgagctt accaccgacg ccctgtgaac cttttaaata ggtattgatc ggatcaattg
     2401 agagattagc aaactccagt cggccagact gtaattcgcc tcgtacaacc tgcccggtga
     2461 gtgtcaaaat cccttcattc aaacgcaatc tgaccgcatc tacgttgagc gtatcctttt
     2521 ttaacgaaaa ggaaaaaggg tcaatatttt ggagaacatt gccactacgc aagatagtaa
     2581 tactatttaa attgaaatcc gtgaattgga cgatatagcc tttagcttct acatccagat
     2641 tttcaccgat ggcttttaaa gtctgaacta tagtggtatc accatttata tgaataatta
     2701 acgaagttaa aggtattttc tccgggatta aattggtctg gcagttggta gcttcaacga
     2761 aaatgtcgcc aaattttctg gtgcgaacat caacaatgcc aaaccgagcc atggtttctt
     2821 caaatttcaa attcgggagt ccaaactgtt tagcgtgaat ccaacctcgt aagtccggtg
     2881 cagtcaaaat tccagtagct tcgacaaaac catcgagaac tccgctcaat gtatccattt
     2941 ttagcatagg agctaacatt tcagcattta aatctgaaaa atgggcacga gcatcaatac
     3001 tattaccatt caaatcagct tcgccttcga ctatcaattt agtttcacca agcacacagt
     3061 ataacgaatc taaaacggtc aataatccat ctttatagac taactctcct cgaatcagat
     3121 tgaaatccaa gggtgcgatt ttactttctt gtaaatctat ttttactttg acctggtcta
     3181 tattcggaat agaaccaaaa gtctgtacgt gtaaattgcc ttgcaaatca gtatttatcg
     3241 ccgatagccc ccacgagctg gcgtctattc cgctaaaacg caattcggcg gttaagcttg
     3301 aatcgagatt accaacaacg gtgccgtgaa cctgctccat ccctttggta aaagtcattt
     3361 ccgccaaatt gacctgtttg ccatcaatca aaccagtaat tcggccgttc gtcaataatt
     3421 gattatgcca agttccagaa aatcccaaat ccagtttgat tgttttatca ctggttttaa
     3481 tctctccgct caggtcgaaa atatcatctt ggcggaatac ctgactgaga gccggtaatc
     3541 gttcagccgg attaatgtca gtaacattga ttcttaactg gctggcgtaa ggtttattca
     3601 acgatattcg accgtccaat tccacagtcg ttccgcgatt gctgatttga ccatttttaa
     3661 tttccagaga attgttgaca aacgctattg cggtctgctt aatcctaaaa tattcgttta
     3721 ttcctgtcac ataaaaatca gtcgaattga gctggagatt aaaagtatca gcattcaggg
     3781 acagcgagcc gttaagggaa tcaatggata ctaataattg cccgggatgg tgactgtcgt
     3841 caatcaaaca tttactcatc aggatatttg ccagcttaaa attaccggtt gacgctaggg
     3901 tatccttttt ctctgggaag ctggctaata aagaatcaat gtttgcagga taagccaact
     3961 tgatagtgtc aagactcaat ttattcaata tatgatgatg accgattaac gattttaaat
     4021 tatatgacaa attgacaccc gacatcgtaa ccagctcggt ttgattttga ttggtcagtt
     4081 ttaatcggtc aaattgtaga tgatagaaaa tattgccacc gattccctcg ggctcaattt
     4141 tcagattata gttctgcatt aattgccgat tgacagaact gactaaaatt gttttccagg
     4201 aatcggtttt tagaattaaa aacccagtca ggccgatcaa taagaccagc gaaactatta
     4261 ctattattga tattttgatt attttcttca aaaaaacctc aacaccgggc aaatttatcc
     4321 actcgttctt aattctccaa tatcgtcaga aaccgtacac aagactattt aatcagtcac
     4381 attgccggtt gttccattaa aagaatagga tttaccggca attgacagta agtggaaaaa
     4441 ttagctgaat aaagttgcct aaccgaaaat tatgtcaaat cctgggcttt ttcctgactt
     4501 tgctcctgat acatctcatc aatcagatca gcataccttt cagcaatggc ctttttcttg
     4561 actttcaagg aaggagttaa ttcaccggat tcgatcgtga aatcctcagg aataatgcga
     4621 aactttttca ctttttcaaa gcgggaaaag tgagctgttt gtctttctat ttcgttagca
     4681 atcagggtga tcaattccgg ccgacgaaca aaatcagcat aattgtcaat tgaaagatgt
     4741 tgattttcgg cccattttcg aacggcttct tgatcgaggg tcaccaaagc gacaatataa
     4801 ctgcgccgat cgccaaacaa aaccgcttcg gaaatgtagg gacttaattt aatcgcatcc
     4861 tcaattggct gaggcgcaac gttcttccca ccggcagtga tcaagatatt ctttttacga
     4921 tcggtgatga ataaaaatcc atcctcatcg aaataaccta catcgccggt gtgtaaaaag
     4981 ccttcttcgt ctaaaacttc ccgagtagcc gcttcgtctt tgtaatagcc cttcattact
     5041 tgtggtccgc gaacgagaat ttcactatct tccgcaattt taaattcggt ttccgggatt
     5101 gcgactccga ccgaacccag acggacttta tccggaccgt tgagagttat caccgggctg
     5161 gtttcggtta aaccgtaacc ttccagaacc ggtaaaccgg ctgagaataa aaactcagcg
     5221 atttccttag aaagcggtgc gccaccggag acaaaccgct ggattttgcc accggttgtt
     5281 ttctgcagtt tgttaaaaac cagcttatcg gcgattttga ttttcctttt ttgccagaaa
     5341 gtcaacggtg ttttgtcgat ataggaatgc attcggtttt taccagcttt caatgcccaa
     5401 aaaaagacct ttttctttat ggctgagccg gattctattt gtgacatcac cgtcgcgtaa
     5461 atcttttcaa acagacgggg aacactgacc aagattgtcg gctgaacttc agccatattt
     5521 tgaactacgg tatcaatatt ctcggcaaag ccgattgtgt tgcccataag cataggcgtg
     5581 tagtatcctc ccatcctttc caatacgtgg ctaagcggca aaaatgatag gaagcaatct
     5641 tccggcacca ttttcattat accaacacta ttgtcaacat ccttgacaaa atttttattg
     5701 gtcaaaagtg cgcctttggg ggcgccggta gtgccggatg tgtaatttat acaagcgata
     5761 tcatcgggct caaccgcatt gagaagagcg ctaaaagcct cgggattctt ggcatccaac
     5821 ttacgtcctg tttctaccat ttccgcaaaa gccactgctt gcggtagctc gggatcggga
     5881 tcataaatca caatacgttc aaattggtaa ccggtgacca tagctttttt cagtaaagtt
     5941 ccattagcca cgaacaaaac tcgcggctcg gaatgtttga atagcggtgc tatctgatta
     6001 ctaatcaagg ttgggtagat tggtacggaa atagctccca tggattgatt agccatatcg
     6061 gacataaccc attcttcgcg gttctcggac aatatcgcca cgcgatcgcc cttgcgaata
     6121 ccccacgaat cgagagctaa cgccaccgct ttgacaattt ctcctaaatc ccgatataaa
     6181 accggtcggt aatgaccatc aactttggat aaaagcgccg gcttatccgc atacttcttt
     6241 acattctcaa aaaacctgtg cgaaattact aaaggattca tatttacctc caaaatgtaa
     6301 tttgaattta aagaattaat tcaataaatt cagccagttt ttattatcgc gatttttaga
     6361 ggagagatgg ccgagcggct gaaggcagct gcctgctaag cagttgtact gccaaaagcg
     6421 gtacccaggg ttcgaatccc tgtctctccg caagatttta gatgaaat
//
LOCUS       DAOMJU010000103         6192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_13510_length_6192_cov_21.448753, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000103 DAOMJU010000000
VERSION     DAOMJU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13510_length_6192_cov_21.448753"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..1362
                     /locus_tag="PLO58_08550"
     CDS             403..1362
                     /locus_tag="PLO58_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQO74878.1"
                     /translation="MSILVTGSAGFIGFHLAHRLLQEGIEVVGYDNVNDYYDPSLKEA
                     RLRILNDFDKFKFYRADLCDFATLQKVFSDQTIEKVVHLAAQAGVRYSLINPFAYQKS
                     NLEGFLNIIELSKCAKVKNFIYASSSSVYGNNRHLPFSVQDNVDRPISLYAATKKSNE
                     LIAHVYAHLYQLPCTGLRFFTVYGPFGRPDMALFIFTKNILEGKPIDVYNFGQMKRDF
                     TYIDDIVAGLRSAIDKPFPYEIFNLGNHRSENLMDFIHLIEEYSGRKAQINFQPIQPG
                     DVPETYADISTATEKLGFVPRTQIREGIKLFLDWYFEYYHIKK"
     gene            1368..2351
                     /locus_tag="PLO58_08555"
     CDS             1368..2351
                     /locus_tag="PLO58_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HQO74879.1"
                     /translation="MLETLPTDLNNLDKIFQEIQNLHILVVGDLILDQYIWGKVERIS
                     PEAPVPIITVSRKENRLGGAGNVAANLSALECPVTLAGVCASDQAGRDLQTLCHQKKI
                     NSIIATLPERRTSCKARIIAQQQQLLRIDEETVEPVDQKETEQLLKNITAFSDRFDAV
                     ILSDYAKGLVTEDFIQSILEIFKDKPVLVDPKGFNYQKYRGATALKPNFREFCMAVRH
                     PELALTKIEPLARQLVDELNLMGLIVTLGEQGVFVLDTDRKAALIPTKAREVFDVSGA
                     GDTFIAVFTAVLMVTNDWKIAAVVANLASGIAVGKIGTATVTTAEILAEDY"
     assembly_gap    2369..2468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2469..3805)
                     /gene="cysS"
                     /locus_tag="PLO58_08560"
     CDS             complement(<2469..3805)
                     /gene="cysS"
                     /locus_tag="PLO58_08560"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQO74880.1"
                     /translation="MSIYFYNTLSRQKEEFRPIEEGVVRLYTCGPTVYNFAHIGNYRA
                     FVFEDLLRRFLKFKGFRVIQVMNITDVDDKIITAANREGVSIREFTEPFRQAFMEDLT
                     TLGIEPAEYYPRATEHINEMVALIETLLKKGVAYRSEDGSIYFKVSEFPRYGQLANLN
                     VTEMRRGDRVANDSYEKEEIRDFALWKAWKPEDGAVFWETSLGKGRPGWHIECSAMSM
                     KYLGRHFDIHTGGVDNIFPHHENEIAQSEAATGEKFVNYWMHCEFLLVNDEKMSKSIG
                     NIIYLRDLVKKGYNPTAIRLTLLGTHYRQKLNFSFEKLEESGKIITRLRDFKKSLEGE
                     FPMGNAGAMEIVEKGEADFEAALDDDLNISNALAAVFKLLHEINLYRQKTPLTQADVE
                     LIRQSLKRIDSVLNILPEPLDMLSEEERKLIDARNQARRERNWAEADRLRNLLL"
     gene            complement(3807..4403)
                     /locus_tag="PLO58_08565"
     CDS             complement(3807..4403)
                     /locus_tag="PLO58_08565"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HQO74881.1"
                     /translation="MSDKKNVDPELIKSNSNSLDTKGKHWYAVYTRPRHEKKVNGLLQ
                     EQQIESYLPLVSTKRQWGNRWAQVEEPLIRSYVFVHIRLNQTLYVLETYGVVRFVMFA
                     RELAVVPDFQIEALKKSLESGFTLSPINYMQVGQLVEVTEGPLKGVIGKIQQIGKNER
                     FVLTLDAVQAAYTIQIDPQWLRPLSPEKKKIFTLPLGF"
     gene            complement(4400..5389)
                     /locus_tag="PLO58_08570"
     CDS             complement(4400..5389)
                     /locus_tag="PLO58_08570"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQO74882.1"
                     /translation="MKIAVLMGGVSSEREVSLSSGRNIVAALRENGHEVLALDTVLPI
                     EQLSQDIQPTSEHLQKGEENLISLLSNATVRAVDFVFNALHGGSGENGAVQSVLEIMG
                     YKYNGSDHEGCAIAMDKIVSKMIFEGIGIRTPEWISFDLKVGYDPVAIQEEILRKIEP
                     PLVIKPAHEGSTVGLSIVKEVAGIQPAIATAFKYNRALLVERYIPGREITIAILGEMA
                     LPLVEIRPKHGIYDYECKYTSGMSEYIVPALVDEKIVKQIQEQALVAFKALRCSGYGR
                     FDLRLTDEGNAFFLEFNTLPGMTATSLVPKAAKAIGMNFNALLEKIIEIGRDR"
     gene            complement(5389..5949)
                     /locus_tag="PLO58_08575"
     CDS             complement(5389..5949)
                     /locus_tag="PLO58_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HQO74883.1"
                     /translation="MNRAVFLDRDGTLNYDSRDYIKNVAEFQLFPITVAALSKLYSAG
                     FKLIVITNQACVAKGLTTVAAVEEIHQLLRKKLSEAGIELAGIYYCPHNPEAGCDCRK
                     PRIGNILLAARELEISLSDSYFVGDSHRDIEAGRAAGCRTILVTTGVRQSSYADIAEW
                     QVQPDYVVPDLLTAAEMILSLERKMN"
BASE COUNT         1699 a         1453 c         1216 g         1724 t
ORIGIN      
        1 ttgtgttcat caaaacctct tataatatat tgatgttaaa agttcaatca taagtaaaaa
       61 gctgctctta aaagtataaa agtgtagttt attaaatcga tctaatgtag aatattttaa
      121 taaaacttcg gcttatttta aagctatttt tgggaaatat tggtgaattt tctgtattta
      181 attccgaaaa accacccaat ctccaagaaa tcaactcgtt atgttagcaa tcataaattc
      241 acacccgata acaattacta ctcacttgac atctgttttg ggacaaatgt taccaaattt
      301 gttcaagcgc tcacccattc ctactttttt tgtatgaaca aatcatttga atttctcaca
      361 taattggttt aatcttttgc gtttattttt aggtgtgatt aagtgagtat tctcgtaact
      421 ggaagtgccg gatttatcgg ttttcattta gctcatcggc tactgcagga gggcatagaa
      481 gtcgtcggat atgataatgt caatgattat tacgatccgt cattgaagga agcccgtctg
      541 cgaattttga atgactttga caaatttaaa ttttatcgtg ccgatctgtg cgatttcgcc
      601 acccttcaaa aagtctttag cgatcaaacg atcgaaaaag tggtccatct tgccgcccaa
      661 gctggggtac gctactcgct gattaaccct ttcgcttacc agaaatcgaa tctggaaggt
      721 tttttgaaca tcatcgaatt atcaaagtgc gctaaggtca agaatttcat ttatgcttcc
      781 agttccagcg tttacggcaa taatcgccac ctgccgttct ccgtccaaga caatgttgat
      841 cgcccaattt cgctctacgc cgccactaaa aaatccaatg aattaatcgc acatgtttat
      901 gctcatcttt atcaactgcc ttgcaccggc ttgcgtttct ttacggttta cggtcctttt
      961 gggcgaccgg atatggcgct gtttattttc actaaaaata ttcttgaggg taaaccgatt
     1021 gacgtctata attttggtca gatgaagcgc gattttactt atattgatga tattgtcgct
     1081 ggtttgaggt cagcaattga caaacctttt ccgtatgaaa tttttaatct cggaaatcat
     1141 cgcagtgaaa acttgatgga tttcattcat ttaatcgaag aatactcggg gcgaaaagct
     1201 cagataaatt ttcagccgat tcaacccggc gatgtaccgg aaacctatgc cgacatctcc
     1261 accgctacgg aaaaactcgg cttcgtgccg cgcacgcaga tccgagaagg catcaaatta
     1321 ttcctcgatt ggtatttcga atattatcat attaaaaagt agaaaatatg cttgaaacac
     1381 tacctaccga tttgaacaac ctggataaaa ttttccagga aatccaaaac ctgcatattc
     1441 tggtggttgg cgacctaatt ctcgaccaat atatttgggg taaggtcgaa cgcatttcac
     1501 cggaagcgcc cgtgcctatt ataactgtca gccgtaaaga aaatcgcctt ggcggagccg
     1561 gcaacgtcgc cgccaacttg agcgctttgg aatgtccggt aactttagct ggtgtgtgcg
     1621 cttccgatca agccgggcgg gatttacaga ctttatgtca ccagaaaaaa attaactcaa
     1681 ttatcgccac attgcccgaa cggcgtacat cttgcaaagc gcgaatcatc gcgcaacagc
     1741 aacaattgct ccgcattgac gaagaaaccg tcgagccggt tgaccagaaa gagaccgagc
     1801 aacttcttaa aaatattacg gctttttccg atcggttcga tgccgtaatt ctttccgact
     1861 acgccaaagg tctggtcacg gaagacttta tccaatccat tctggaaata ttcaaggata
     1921 aaccagtact ggtcgatccc aagggtttca actatcaaaa atatcgtggc gctaccgccc
     1981 tcaagcctaa tttccgtgaa ttttgtatgg cggtgcgtca tcccgagcta gccctcacta
     2041 aaatcgaacc gcttgctcga caattggttg atgaacttaa cctgatgggc ttgattgtta
     2101 ctctgggtga acaaggggtt ttcgtactcg atacagaccg gaaagccgct ctaattccca
     2161 ctaaggcgcg cgaagtcttc gatgtcagcg gtgccggtga tacttttatc gccgttttca
     2221 ccgccgttct aatggttact aatgattgga aaatagcagc tgtagtagct aatttagcat
     2281 ccgggattgc cgtcgggaaa atcggcacgg ctaccgtcac aaccgctgaa attctcgctg
     2341 aagattatta gctattcacc actaaggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnag gagaaggttt cgcaagcgat cggcttccgc ccaattacgt tcccgccggg
     2521 cttgatttct ggcatcaatt aacttccgct cttcttcaga aagcatgtca agcggttcgg
     2581 gcagaatgtt cagaacggaa tcaattcgtt ttagagattg tcgaattaat tctacatccg
     2641 cctgggtcag cggagttttt tggcgatata aattgatttc gtgcaataac ttaaaaacag
     2701 ccgccagggc attggagata ttcagatcat catcgagcgc cgcctcgaag tcggcttcac
     2761 ctttctcgac gatttccata gcaccggcat ttcccattgg gaattcacct tccagcgatt
     2821 tcttaaaatc gcgcaagcgc gtaatgatct tgccggactc ttcgagtttc tcgaaactga
     2881 aattcaattt ctgccgatag tgggtgccca gcaaagttaa cctaatagca gtcggattat
     2941 atcctttttt gaccaaatcc cgcaggtaaa taatattgcc gattgacttc gacatttttt
     3001 catcatttac cagtaaaaat tcgcaatgca tccaataatt aacgaatttc tcgccggtgg
     3061 cggcttcgct ctgggcaatt tcattttcat gatgaggaaa aatattatca acgccgccgg
     3121 tatgaatatc gaaatgccgt ccaagatatt tcatactcat cgcggaacat tcgatatgcc
     3181 agccgggacg ccccttgccg agcgacgttt cccagaaaac tgcaccgtct tccggtttcc
     3241 aggctttcca gagtgcaaaa tcacgaattt cttctttctc gtaggaatca ttggctactc
     3301 gatcgccacg ccgcatttcc gtaacattca gattggctaa ctgcccatag cgcggaaatt
     3361 cggaaacttt gaaataaatc gaaccatcct cagagcgata agcgacaccc tttttcaaca
     3421 aagtttcaat caatgccacc atctcattga tatgttcggt agcgcgcgga taatattcag
     3481 ctggttcgat tcccaacgtc gttaggtctt ccataaacgc ctgccgaaag ggctcggtaa
     3541 attcgcgaat ggaaacaccc tcgcgattcg ccgctgtgat aattttatca tcaacatcgg
     3601 taatattcat tacttgaata acacggaaac ctttgaattt caggaatcgt cgcaataaat
     3661 cttcgaaaac gaaagcccgg taattaccga tatgagcaaa attatagacc gttggcccac
     3721 aggtataaag gcgaaccaca ccttcctcta tcggacggaa ttcttccttt tggcgactca
     3781 gggtattata aaaataaatg ctcatattaa aatccgagtg gtagcgtgaa aattttcttc
     3841 ttctcaggac ttaacggacg aagccactgg ggatcaatct gaattgtata agcggcttgc
     3901 accgcgtcga gcgtcagaac aaatcgctca tttttgccga tttgctgaat ctttccgatt
     3961 acgcctttta atggtccctc ggtaacttca accaattggc caacctgcat ataattaatt
     4021 ggtgaaagag taaaaccact ctccaggctc tttttcaaag cttcaatctg aaaatcgggc
     4081 acgaccgcca gctctcttgc aaacattaca aagcgcacca cgccgtaggt ttccagaaca
     4141 taaagcgtct gattgagacg aatgtgaaca aagacgtagc tacgaatcaa tggctcttca
     4201 acctgagccc agcggttgcc ccattgtcgc ttcgtggata ctaaaggtaa ataactctca
     4261 atctgttgct cttgcagaag tccattgact tttttttcat gtcgcggtcg ggtatagact
     4321 gcataccaat gtttaccttt ggtatccaaa ctattggaat tggatttaat caactccggg
     4381 tcaacatttt ttttatcgct catctatccc taccgatttc tataattttt tcaagtagag
     4441 cattgaaatt cataccgata gctttggcgg ctttcggtac taaactggtc gccgtcatac
     4501 cgggcagggt attaaattcc aggaaaaagg cattgccttc atcagtcaga cgcaaatcaa
     4561 aacggccgta tccagaacaa cgtaaagctt tgaaagccac taacgcctgt tcttgaatct
     4621 gcttaacaat tttttcgtcc accaaagccg gcacaatata ctcactcata ccggaagtgt
     4681 atttacattc ataatcataa atgccatgct tgggacgaat ttcaaccagc ggcaaagcca
     4741 tttcaccgag aatcgctatg gtaatttcgc gcccgggaat atagcgctcg accaacaaag
     4801 cccggttgta tttaaaggcg gtagcaatag ccggttgaat cccggcaacc tcttttacaa
     4861 tggagaggcc gacggttgag ccttcatggg cgggcttgat gaccagcggc ggttcaattt
     4921 ttcgtaatat ttcttcttga atcgcgaccg gatcgtaccc gacttttaga tcgaaagaaa
     4981 tccattccgg cgttctaatc cctatgccct cgaagatcat cttcgaaaca attttatcca
     5041 tagcaatcgc acaaccttca tggtcactgc cgttatattt gtaacccata atttcgagta
     5101 cgctttgaac cgccccgttt tcgccactgc cgccatgcag ggcattaaaa acgaaatcca
     5161 cggcgcggac ggtcgcattg cttaataaac taatgaggtt ttcttcgcct ttctgcagat
     5221 gctcgctggt tggttgaatg tcctgactta attgctcaat cggtaaaact gtatcgagcg
     5281 ccagcacctc atgcccattt tcccgtaaag ccgccacgat attgcggccg ctggagagcg
     5341 atacctcccg ttcggacgaa acaccaccca tcaggacggc gattttcatt aattcatttt
     5401 cctttctaaa cttaaaatca tctcagccgc agtcagtaaa tcgggaacca cataatccgg
     5461 ttggacttgc cattccgcaa tatcggcata actggattga cgaacaccag ttgtgactaa
     5521 aatagttcga caaccggcgg cgcgtcccgc ctcgatatct cgatgtgaat cccccacaaa
     5581 gtacgaatct gatagactaa tctcaagttc acgggcggca agaagaatat taccaatccg
     5641 tggcttgcga caatcacaac cggcttccgg attatgcgga caataataaa taccagccaa
     5701 ctcaatgccg gcttcgctta attttttccg caatagttgg tgaatttcct cgacggcggc
     5761 gaccgttgtc aagcctttag cgacgcaagc ctggttggta attacgatta atttgaatcc
     5821 agcagaatat aacttactta aagccgcgac cgtaattgga aaaagctgaa attccgcaac
     5881 gttcttgata taatcgcggc tatcataatt cagggtgccg tcccgatcta aaaaaactgc
     5941 tctattcact ttaataaaat tacctgtcct aaatttagcg ccgatgctgt ggataagttt
     6001 gtcaacttaa ataacataaa ataaactatt acctgacttt ggcatacttg gctgtcctcg
     6061 ccttctacat cctcaaacat cgctattgac actcaccttc atttctccat aaaaatccag
     6121 acataaatta caatccggag gcgtaaaata atagggcagg gcataaaaaa acctgcagaa
     6181 ggttttattt at
//
LOCUS       DAOMJU010000104         6153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_13628_length_6153_cov_19.963103, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000104 DAOMJU010000000
VERSION     DAOMJU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13628_length_6153_cov_19.963103"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLO58_08580"
     CDS             complement(<1..563)
                     /locus_tag="PLO58_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase"
                     /protein_id="HQO74884.1"
                     /translation="MTFLNFLFPPDDPLSDILKKISTVAECNHKRVFLVGGVVRDRLL
                     GIDTKDIDISVIGNGIEFAHKLAKSLKVDNVIEYPRFGTAMIPYHDKLIEVATARMEE
                     YETSSRKPQITESSLRDDLARRDFTINAMALSLNRENYLELTDYFEGLKDLNAGIIRT
                     PLDPITTFSEDPLRMLRAIRFATQLNFR"
     gene            complement(798..>1793)
                     /locus_tag="PLO58_08585"
     CDS             complement(798..>1793)
                     /locus_tag="PLO58_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQO74885.1"
                     /translation="GCSSIYSGSVVCPYCVNETGYGPAWANSLFEDNAEYGFGMALAI
                     IQRREYLAEIINEALKSADGELAEAFKAWLDGKDDAELSRAAGDRIKAALADKWEGNK
                     LLTEIWKNRDILTKKSIWIIGGDGWAYDIGYGGLDHVIAMGYDVNILVLDTEVYSNTG
                     GQSSKATPLGSVAKFAYSGKKTSKKDLGMLAINYKNVYVASVAMGASANQFLKSALEA
                     EKYPGPSLIIAYCPCINHGIYKGMLSQEESRQAVECGYWPLYRYNPLLKAEGKNPFQL
                     ESKEPNGKLMEFLDGEVRYATLKKSFPEESEKLRKALSEEVMERYQRYKRMAEGS"
     assembly_gap    1794..1893
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1894..4139)
                     /gene="nifJ"
                     /locus_tag="PLO58_08590"
     CDS             complement(<1894..4139)
                     /gene="nifJ"
                     /locus_tag="PLO58_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQO74886.1"
                     /translation="MSKTMKTMDGNTAATHIAYALSEVAAIYPITPSSVMGELADEWS
                     AQGRKNIFGQPLRVVEMQSEAGAAGAVHGSLSAGALTTTFTASQGLLLMIPNMFKIAG
                     ELMPAVFHVSARAVAGQALSIYGDHSDVMATRTTGFGLMASGSVQEVMDLSLVTHLAS
                     LKASLPFVSFFDGFRTSHEIQKIEVIDYETIKSLVDFDDIDRFRARCLNPEHPQLRGT
                     AQNPDIFFQVAELANKYYDAVPEIVAEMMRKVGEVTGRHYNLFDYAGDPNAERVVVIM
                     GSGSETVQGVAEYLNARGEKVGVLKVHLYRPFSAKHFMAALPKSAKRIAVLDRTKESG
                     AFGEPLYTDVCAVLQREKDNRLVVGGRYGVGQKEFSPAMAKAVFDNLKLPEPKNGFTV
                     GIEDDVTFRSLPVTEDIDTVPEGTVQSIFWGFGSDGTVGANKDAIKIIGDNTDLYAQG
                     YFSYDSKKSGGLTVSYLRFGPKELHTPYRINLADYVACHKQSYINNYDMLVNLKPGGI
                     FVLNTLWDDAELDANLPAAMKRAIATKKVRFYAVDANRIAGEVGLGGRINMVMQTAFF
                     ALSKVLPFEEAIKHLKNAIQKTYGAKGEKIVKMNWQAVDSAINAIREVKYPASWAEAK
                     ETAESPKPVPDFVKKVLQPINAFNGDNLPSSVFTPGGIFPTATTKYEKRGIAIEVPEW
                     IKENCTQCNQCSFVCPHAVIRPVLVTPEEKAKAPTTFETIKAIGKGLENYEYRMQVSV
                     LDCTGCGN"
     gene            complement(4572..5762)
                     /locus_tag="PLO58_08595"
     CDS             complement(4572..5762)
                     /locus_tag="PLO58_08595"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HQO74887.1"
                     /translation="MKKRIGILTGGGDVPGLNPAIRGITLRALKEGFEVIGIRRGWAG
                     MINIIRDKEADNSQNFIMLNQEIVNKAGRTGGTFLHTSRTRPSSVKLDAVPEHLRDKY
                     TAEVNDLTPEVLKNLDFLGIDYLIPIGGDDTLSYGVTLHKAGVKVIGVPKTMDNDVPG
                     TDYCIGFSTCVTRTLELTHSLRTSAGSHERFLVIEVFGRYAGFTALLPTLAGAANRCV
                     IPEYKFNMERLTELLVQDRFTNPSRYSVVLVSEGAMMQNQDQMMFQSEETDAFGHKKL
                     GGIGDVVSRQLKELSGKYNNGHKINVISQKLGYLVRCGDPDALDSIVPMAYGNIAFEL
                     IMNGRSGLLVNLRNGVYGHTDLEVVTSTKKVVDVEKFYETDRLRPKYKSFENKSFFIM
                     TSDK"
     gene            complement(5851..>6153)
                     /locus_tag="PLO58_08600"
     CDS             complement(5851..>6153)
                     /locus_tag="PLO58_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74888.1"
                     /translation="FAAIFAEMTGTLNSGLVMIRVTDPEFKTPVAEDLVYSSGIALFL
                     GLPLLILLNVPMNFFNNSLKGYWITFGLIIVYGVILWIFWRAIGFIHFKKNPKSKV"
BASE COUNT         1714 a         1468 c         1217 g         1654 t
ORIGIN      
        1 cgaaaattca actgcgtggc gaaacggatg gctcttagca ttctcagcgg gtcttcggaa
       61 aaagtggtaa tcgggtccag cggagtacga ataattccgg cattgaggtc ttttagacct
      121 tcgaaataat ccgtcaactc taggtaattt tcccgattca aggaaagtgc catcgcattg
      181 atcgtaaaat cgcggcgggc taaatcatca cgtaaactac tttcggtgat ctgaggtttg
      241 cgcgacgagg tttcgtattc ctccatccga gccgtagcaa cttcgatcaa tttatcatga
      301 tagggtatca tagcagtgcc aaagcgcggg tattcaataa cattgtcaac tttcagactt
      361 tttgccagtt tgtgcgcaaa ttctatccca ttaccgataa cggaaatatc aatgtcttta
      421 gtatcaattc ccaataaccg gtcacgcacc acaccgccga ccaaaaagac gcgcttatga
      481 ttacactcag ccacggtgga gattttcttt aaaatatcag acagaggatc atctggaggg
      541 aataaaaaat taagaaatgt cataataaaa cttttaaaat gctcgtcaat ttaccaacct
      601 ggtcagttaa tcacaatact aatcgaagaa caaatgttcc acgaatacgg atttaatttt
      661 gacggcctgg atgtatggat gcgtggatga gcagagattg aattagaata gccattctga
      721 tttcttataa gcacttataa gcacttaaga gaacttaata tccgagcccc tttctcattg
      781 gagtcgttat attaaactta gcttccttcc gccattcgtt tatatctctg atagcgctcc
      841 attacttctt cggatagggc ttttctcaac ttttccgatt cctctgggaa agatttcttg
      901 agggtggcat aacgcacttc gccatctaaa aattccatta gcttaccatt gggttccttg
      961 ctttcgagct ggaacggatt tttaccttcg gcttttagta aaggattata acggtaaagc
     1021 ggccagtaac cgcattcgac ggcttgtctg gattcttcct gggagagcat tcccttgtaa
     1081 ataccatgat tgatgcaggg acaataagca attatcaaag aaggacccgg atatttctcg
     1141 gcttccagcg cggattttaa gaattggttg gcgctggcgc ccatcgccac cgaagccaca
     1201 tagacatttt tataattaat ggctaacata ccaaggtctt ttttggaagt ctttttaccg
     1261 gagtaagcaa atttggcgac tgaacctaaa ggcgttgctt tggacgactg cccgccggta
     1321 ttagaataaa cttctgtatc gagcaccaaa atattgacgt catatcccat agcaataacg
     1381 tgatcgagac caccgtagcc gatatcataa gcccaaccat caccgccaat aatccagatt
     1441 gatttcttag tcaaaatatc acgatttttc cagatctcgg tcaatagctt attaccttcc
     1501 catttatcag ccagagcggc tttgatccga tcgcccgccg cgcgcgagag ttcggcatca
     1561 tctttaccat caagccaggc tttaaaagct tccgccagtt ctccatcggc tgatttgagt
     1621 gcctcattaa taatttccgc cagatattca cgacgctgaa taatggctaa agccattccg
     1681 aaaccatatt cggcattgtc ttcaaagagg gaattagccc aggctggtcc atatcctgtt
     1741 tcattgacac aataaggaca aaccacgcta ccgctataaa ttgacgaaca accnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncagttac cgcaaccggt gcaatccagc
     1921 accgagacct gcatacgata ctcgtaattt tcgaggcctt tgcctatggc tttaatggtc
     1981 tcgaaagttg taggcgcctt ggctttttct tccggagtga ccagtaccgg acgaattaca
     2041 gcatgtgggc agacaaaact gcactgattg cattgggtac aattttcctt gatccattct
     2101 ggaacttcga tggcgattcc gcgcttttca tatttggttg ttgccgtcgg gaaaatgcca
     2161 cccggcgtga atacgctact tggcaaattg tcaccattaa aggcatttat cggctgtaaa
     2221 acttttttaa caaaatccgg tacaggtttt ggcgattcgg ccgtctcttt ggcttccgcc
     2281 caagacgctg ggtattttac ttcacgaatg gcattgatgg cagaatctac agcctgccag
     2341 ttcatcttga cgattttttc acccttggcg ccgtaagttt tctgaatagc attttttaag
     2401 tgcttgatgg cttcctcgaa aggcaaaact ttggacagcg cgaagaaggc tgtctgcatt
     2461 accatattga tgcgacctcc gagtcccact tcaccggcaa ttctattggc atctaccgca
     2521 tagaaccgaa ctttcttggt tgcgatcgcc cgtttcatag cggctggcaa attagcgtcc
     2581 agctcggcgt catcccaaag cgtattcagt acgaaaatgc cacccggttt aagattaacc
     2641 agcatatcgt aattattgat ataactctgt ttatgacagg cgacataatc tgccagatta
     2701 atccgatagg gtgtatgtaa ttcttttggt ccgaaacgca aatagctgac cgtcaaacca
     2761 cccgattttt tcgaatcgta agagaaatag ccctgcgcgt ataaatcggt attgtcgccg
     2821 ataattttaa tcgcgtcctt attggcaccg accgttccat ccgagccaaa accccagaaa
     2881 atagattgga cggtaccttc cggcacagtg tcaatatctt cggtaactgg caaactgcgg
     2941 aaggttacat catcttcgat gcccaccgta aaaccgtttt tcggctcggg cagtttcaga
     3001 ttgtcgaaaa cggcttttgc catcgccgga ctgaattctt tttggccaac gccgtagcgc
     3061 ccgccaacga ctaagcgatt atctttttct cgctgtaaaa cagcacacac atcggtataa
     3121 agcggttcac caaaagcgcc ggattctttg gtacgatcca gcacagcaat ccgcttagca
     3181 ctcttgggca aagccgccat aaaatgctta gccgagaagg gacgatacag gtgaaccttt
     3241 aatacgccga ctttctcgcc tctggcattt aagtattcag caacaccctg aacggtctcg
     3301 gacccggaac ccattataac caccactcgc tccgcattcg gatcaccggc ataatcgaat
     3361 aggttataat gacggccggt aacttcccca actttccgca tcatttcagc gacgatttcg
     3421 ggaacggcat cgtaatattt attagccaat tcagctactt gaaaaaagat gtccggattc
     3481 tgagccgtgc cacgtaattg cgggtgttct ggattcaaac agcgggctcg gaaacgatca
     3541 atgtcatcaa aatcaactaa tgacttaatt gtttcataat ctatgacctc gattttctga
     3601 atttcatggc tggtgcgaaa accatcaaag aaactgacaa acggcaggct ggctttcagc
     3661 gaagctaaat gagtaaccag tgataaatcc atcacctctt gcaccgatcc ggatgccatt
     3721 aaaccaaaac cggtcgtgcg agtagccata acgtccgaat gatcaccgta aatcgagagt
     3781 gcttgcccgg ctaccgctcg cgccgaaaca tgaaacacgg ctggcatcaa ttcaccagca
     3841 atcttaaaca tattaggaat cattaataga agaccctgcg aagcggtaaa ggttgtagtc
     3901 agcgcgccag ctgaaagact gccgtgcacg gctcccgccg caccggcttc actctgcatt
     3961 tcaaccactc gcaggggctg tccgaaaata tttttccgac cttgcgccga ccattcgtca
     4021 gccagttctc ccatcacaga cgatggtgta atcggataaa tagccgcaac ttcactaaga
     4081 gcataggcta tatgagttgc cgctgtattg ccatccatag ttttcatagt tttagacatg
     4141 gtgattctcc attgaatatt ttaatgttta taaaattatt tgaattattg ctgggttttt
     4201 ttgaattagt aaattcatca ctttcaaaac taaagaaagt tacgaccgaa atgcccagaa
     4261 aagcaaccgc tatctctatc agaaagtaac tgcagagcca cacgtatttt ttcgagaaaa
     4321 aatgaaggga atattttcaa ttagtcttaa aaagagagga aagattgacc attcttaacc
     4381 taatgctatc catttcatca tcataatcag tttcaacacc ataaatcagt taagggataa
     4441 attatggttc caataaaaaa gtgaagccca aaaaatatca ccttgccgct ccttttaaaa
     4501 gcaaaaaggg caatcatgcg attgcccttt tgcggtgtcc tcatagagag gagggaggag
     4561 ggaaactttg tttacttatc gctggtcatg ataaaaaacg atttgttttc aaaactctta
     4621 tacttaggac gcaggcgatc ggtttcataa aatttttcaa catctaccac ttttttggtg
     4681 ctagtaacca cttccaggtc cgtatgacca tagacaccat tacgcaagtt gaccagcagt
     4741 ccggaacgac cattcataat caactcaaat gcgatattac cataagccat cggcacgatc
     4801 gaatcgaggg catctggatc accgcatcgc accaaataac cgagtttctg gctgatgacg
     4861 ttaattttat gaccgttatt gtattttccg gatagttctt taagttgcct tgaaactaca
     4921 tccccgattc cgcccagttt tttatgacca aaggcatcag tctcttcgct ctggaacatc
     4981 atctgatcct gattctgcat catagcacct tctgaaacta gaaccaccga atagcgactg
     5041 ggattagtaa agcgatcctg caccaatagc tcggttaagc gctccatatt gaatttatac
     5101 tcgggaatta cacagcggtt ggcagcccca gctagagtcg gaagcaaagc cgtaaaaccg
     5161 gcatagcgtc cgaaaacttc gattaccaga aaacgttcgt gtgaaccggc tgaagtccgc
     5221 aaactatgag tcaactccag cgtccgcgtc acgcaagtac tgaaaccgat gcaataatcc
     5281 gtaccgggga catcattatc catagttttg ggaacaccaa tcaccttgac ccccgcttta
     5341 tgcaaagtga cgccgtagct cagggtatca tccccgccga ttgggattag ataatcaatc
     5401 ccgagaaaat cgaggttttt aagtacttca ggggtcagat cattaacctc agctgtatat
     5461 ttatcccgca aatgctccgg cacagcatcc aatttaacac tacttggccg cgtgcgggaa
     5521 gtatgtaaaa atgtcccacc ggtgcgcccg gctttgttga cgatttcctg attcagcatg
     5581 atgaaattct ggctgttgtc agcctcttta tctcgaatga tattgatcat ccccgcccag
     5641 ccgcgtcgga taccaataac ctcaaagccc tcttttaatg cccttaaagt aatgccgcga
     5701 attgccggat ttaaaccagg tacatcgccg ccacctgtca atattccgat tctctttttc
     5761 aattaatgct ccttatattg tttcaactaa aacagatcat caaatcggat tcaattttaa
     5821 cataaattaa gcaaattttc aacaatctta ttaaactttg gatttaggat ttttcttaaa
     5881 atgaataaaa cctattgccc gccagaatat ccataaaatt acgccatata caattataag
     5941 cccgaaagta atccagtaac ctttgagact attattgaaa aaattcatcg gcacatttag
     6001 cagtattaaa agtggcaaac ccagaaatag cgcaatgccg ctggaataga ccaaatcttc
     6061 ggctaccggc gtcttgaatt ccggatccgt tacccgaatc attactaggc cggagttcaa
     6121 cgttccggtc atttccgcaa aaatcgccgc aaa
//
LOCUS       DAOMJU010000105         5804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_14767_length_5804_cov_17.773004, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000105 DAOMJU010000000
VERSION     DAOMJU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14767_length_5804_cov_17.773004"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..98)
                     /locus_tag="PLO58_08605"
     tRNA            complement(26..98)
                     /locus_tag="PLO58_08605"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Ala,seq:ggc)
     gene            complement(150..1070)
                     /locus_tag="PLO58_08610"
     CDS             complement(150..1070)
                     /locus_tag="PLO58_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74889.1"
                     /translation="MTLDAGNLFFDRDEIETKQLELKQETARLIVDIYNNLKYDAYNV
                     GGHDFALGLDFLKELQAKARFTFISANVVDTLTGKPVFEPYKIVKVGNKNFGIIGVTS
                     VWNEKYRGVGIADPIQTLNKYLPALRKKADYIIVLAALTAGDENKLGAIKEKIDFILM
                     AGSYRYSRNLEVVDGRFIARCGTTGKYVGIITAEINQPKKPLQDISNISVQLESATNR
                     LKSFKTNAGDKSIEEYYADKPTQLKVIRDLQETQNELLRQKSQIVNPVNYELIELDEK
                     IEDDPAIRKKLNDFERRMNAKGYKITPAGD"
     gene            complement(1146..1271)
                     /locus_tag="PLO58_08615"
     CDS             complement(1146..1271)
                     /locus_tag="PLO58_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74890.1"
                     /translation="MKKLFKLICIILVGLLSQVNAAERFAILYTGSVMGETEPCG"
     gene            complement(1268..2323)
                     /gene="fni"
                     /locus_tag="PLO58_08620"
     CDS             complement(1268..2323)
                     /gene="fni"
                     /locus_tag="PLO58_08620"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HQO74891.1"
                     /translation="MIHKITERKKDHLRIICEENVQYEKVTTGFEQFTFIHNALPEID
                     LREVDCTCEFLGYQLQIPLIICPIGGGESDGKELNKALAKVANKARIALGIGSLRPAL
                     IDKSVLGTYAVVRENAPDIPLIANLGAVQLAEGIDLQTLKLLLNEIGADALSIHLNPL
                     QEALQPEGDPAFHNVSRAIEILKDTLPIPIIVKEVGFGLSSGVIRRLGKMGIKWIDVA
                     GAGGTSWSRIEAKRITDSYRKRLAAEFFEWGIPTVSALLEAVKIKGINVIASGGIETG
                     LQFAKAIALGATLGGGAALFVRTWYAQGIEGLAETVNLFGDTLRHAMFCTGCKTLGTF
                     RGNSEIIKNNSNLSNVK"
     gene            complement(2382..3137)
                     /locus_tag="PLO58_08625"
     CDS             complement(2382..3137)
                     /locus_tag="PLO58_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQO74892.1"
                     /translation="MTKTILITGTTSGFGKSIAERFAAEGWNLILIGRRSDRLMAISA
                     KLGEKVTVLPITLDIRQRQAVMETLTNLPEKFKAVDVLVNNAGLAAGLEPAQSADLDD
                     WDAMVDTNIKGLMYVTRAILPGMVTRNAGHIINIGSVAGSYPYEGGNVYGATKAFVAQ
                     FSRNLRTDVSGTAIRVTNIKPGNAETEFSLIRFKGNVAKAQAVYQGYQPLTAEDIAEM
                     VFWVTHLPSHVNVNELEVMPVAQSWAGYKIHRQ"
     gene            3397..4830
                     /locus_tag="PLO58_08630"
     CDS             3397..4830
                     /locus_tag="PLO58_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQO74893.1"
                     /translation="MANQRKRKVENGLVEGRLSRSIWKLAAPMMIGGALLDLFSIVDL
                     FFVGRLGHIEVAALSIAGTVMAIMMMLVQGIGVGTTALISHFTGEKNYEKSDEVLGQT
                     LILGLIGSAIMLVVSFFLIEPLLKLFGATGEVLTYAADYLRINFNYSIIIFLFSGVNS
                     ALQGSGDVRTPLNALIIGNVLNIILDPLLIMGYGPFPAMGVSGSAVATVITRGIGLIY
                     LFGHVLFGHSTIHLGKRHFKPDPALMQRIVSIGFYASLQVFIREISFLFLMRLVTSYG
                     DVTLAAYGIGSRIRSFIMVPGFGFASAAAVLVGQNLGAGKPERAKKSAWRAVIYYETI
                     AVPVALIFLIFAPTIVGIFNDHPEVITIGSSFLRYLAVTFPFLAFSLVFSQALNGAGD
                     TRTATIVNAIGQLGFRIPLAYFFARTVGLGYTGVWLGINASDVVQGIGMILVFNAGHW
                     QKVFLKHKKKLAYDLEPREIITELPTN"
     gene            5026..5799
                     /locus_tag="PLO58_08635"
     CDS             5026..5799
                     /locus_tag="PLO58_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQO74894.1"
                     /translation="MIIKMVLVGIFTGIVTGLTGASGVMVVVPLLSILTDFSVHEAIG
                     TSLIVDVIAPLAISYVYFKHKNIDIKSGIWIAVGSIIGAQLGASFAGGISEVGLGSTF
                     GIFMVIMGILIWKKGLNHESLAKVMKKVAKFETQTQRIVTALILGFLVGIMTGVLGAG
                     GGGMILLILIFVLNFPIHIAIGTSALIMAITACSGAIGYALHGNTKPVEGLILGISAA
                     GSGMFSAKFANKANEQTLGKIIGVIFLLLGIIMTILNFR"
BASE COUNT         1627 a         1123 c         1235 g         1819 t
ORIGIN      
        1 aagcctgttc tacgttttgg tgatgtggag ctggtcggga tcgaaccggc gacctcttga
       61 atgccattca agcgctctcc cagctgagct acagcccctt acaaaaagcg cgtaaaatta
      121 ctatgaattg tttagtaatg caagccttgt taatcgcccg ctggtgttat tttataacct
      181 ttggcgttca tccgcctctc aaaatcgttc aattttttac gaattgcagg atcgtcttct
      241 attttttcat ccaattcgat taattcataa ttgaccggat taacgatttg acttttttga
      301 cgtaataatt cgttttgagt ttcttggaga tcgcgaatta ctttcagttg agtcggtttg
      361 tcggcataat attcctcgat gcttttatcg ccggcatttg ttttaaaaga cttgagccga
      421 ttggtggcag attccagctg aacgctaata ttggaaatat cctgcaacgg ctttttaggt
      481 tgattaattt cagctgtaat gattcctacg tatttcccag ttgtaccaca gcgggcgata
      541 aacctgccgt caaccacttc cagattacgc gaatagcgat aggaacccgc cattaaaata
      601 aaatctattt tctctttaat tgctccgagt ttattttcgt caccagcggt caaagccgct
      661 aatactatga tatagtcagc ctttttacgc agtgccggca aatatttatt taaagtctga
      721 atgggatcgg caatcccgac accgcggtat ttttcattcc agaccgaagt cacaccgatt
      781 ataccaaaat ttttattacc gactttgacg attttatatg gttcaaaaac cggtttgccg
      841 gtcaatgtat ccaccacgtt tgcggagatg aaagtaaacc gggctttggc ttgtaattct
      901 tttaaaaagt cgagacctaa ggcgaaatca tgtccaccga cattataagc gtcatatttt
      961 aaattattat aaatatcaac gatcagtcgc gcagtttctt gcttaagttc taattgcttt
     1021 gtctcgattt cgtctcgatc gaagaaaagg ttaccggcgt ccaatgtcaa actgttcggg
     1081 taattcgtcc gatatttatt gaaaacttct gcacgcctag ccagaccgcc aagccgatgc
     1141 gttcgtcacc cacagggttc ggtttcaccc ataaccgaac ctgtgtatag gattgcaaag
     1201 cgctcggcgg cgtttacctg gctcagcaag ccgacgagta ttatgcaaat tagcttaaac
     1261 aatttcttca ttttacatta cttaaattgc tattgttttt gataatctct gaattaccgc
     1321 ggaaagttcc caaagtttta caaccggtgc aaaacattgc gtgccgcaaa gtatcgccga
     1381 ataaattcac ggtttcagcc aatccttcga taccctgggc gtaccaggta cgtacgaata
     1441 aagccgcgcc accgcccaga gttgccccaa gagcaatggc tttcgcgaac tgaagaccgg
     1501 tttcgattcc tccggatgca atgacattaa tgcctttgat ttttacggct tccaataaag
     1561 ccgatacggt tggaattccc cattcgaaaa attcagcggc taatcgtttt cgataggaat
     1621 cggtaatgcg tttggcttca atccttgacc aggaagtgcc accggcaccc gccacgtcaa
     1681 tccacttaat tcccattttg cctaagcgtc taatgacccc gctcgaaagt ccaaaaccta
     1741 cttcttttac gataattgga attggcaaag tatcctttaa aatctcaatt gcccgactga
     1801 cattatgaaa tgcaggatcg ccttctggtt ggagagcttc ttgaagcggg ttgaggtgaa
     1861 ttgagagtgc gtctgctcct atttcattca gtaataattt taatgtctga agatctattc
     1921 cttccgccaa ctggactgcg cccagattag caattaatgg aatatccggt gcattctcac
     1981 gcacgactgc gtaagttccc aatacagatt tatcaattaa agccggtcgc aaactgccaa
     2041 tccctagagc gatacgagct ttattggcta ctttcgcgag agctttattg agttctttac
     2101 catcgctttc gccgccgcca attggacaaa ttatcagggg tatttggagt tgatatccga
     2161 gaaattcgca ggtacaatca acctcacgga ggtctatttc tggtaaagcg ttatgaataa
     2221 aggtaaattg ttcaaaaccg gttgttactt tttcatactg aacattctcc tcgcaaataa
     2281 ttcggagatg atctttttta cgctcggtta ttttatgaat cattgcggca aatctattta
     2341 atcgtcagta taattcaaaa ttacgtttat cagtataaat attattgccg gtgaattttg
     2401 taaccagccc aggattgagc aactggcata acttctaatt cgttgacatt gacatgtgag
     2461 ggaaggtgag tgacccagaa gaccatttca gcaatgtctt cagcggtcaa aggctgatag
     2521 ccttgataga ctgcttgagc tttggcaacg ttacctttga accggatcag tgaaaattcg
     2581 gtttcggcat tcccgggctt gatattagta actcgaatcg ccgttccgct gacatctgtt
     2641 cggagattac gactaaactg agctacgaaa gctttagtgg cgccatagac attaccgcct
     2701 tcataaggat aggagccggc taccgagcca atattaataa tatgcccggc attcctcgta
     2761 accataccgg gcaaaatcgc ccgagtcaca tacattaagc ctttgatatt ggtatctacc
     2821 atcgcatccc agtcatcaag atcggccgat tgagcaggtt caagaccggc ggctaaaccg
     2881 gcattgttaa ccagaacatc cactgcctta aatttttccg gtaaattggt gagagtttcc
     2941 ataactgcct gacgctgacg aatatcgaga gtaattggta atacagttac tttttcgccg
     3001 agtttggctg aaatagccat aaggcgatcg gagcgacgac ctattagaat caaattccat
     3061 ccttctgcgg cgaaacgctc ggcgatactt tttccaaatc cggaagtggt tcctgtgatt
     3121 aaaattgttt tagtcatatt tctcctttca taggcgaaaa tttcagagta acaatttaaa
     3181 aatgcaactg gaaaatgcga tcgagaagcg aatacaaatt aaatttaaat tataaaaatg
     3241 tacataaaaa ctattggaaa tctcagctgg caaaattaaa atccaaagtt tgaagatgtt
     3301 tagaattata agaaataaaa atataagtca gaaacattac agaaatgtgt aaatatttga
     3361 cttaccccat tctcttacga tacactaagg aataatatgg caaatcaacg caagcggaag
     3421 gttgaaaatg gtttagtaga aggccgactt tcgcgttcga tctggaaact agcggctccg
     3481 atgatgattg gcggggcttt gcttgacctt ttctctatag tagatttatt ttttgttggc
     3541 aggttgggac atatagaagt agctgctctc tctattgccg ggacagttat ggcaatcatg
     3601 atgatgttag ttcagggtat aggtgtaggg acaacagctt taatttcgca ttttaccggt
     3661 gaaaagaatt atgagaagtc ggatgaagtt ctggggcaga cgcttatttt aggattaatc
     3721 ggctcggcta taatgctggt agtctcgttt tttctaattg aacccctttt gaaacttttt
     3781 ggtgccacgg gtgaagtatt aacctatgcc gctgattatc tgcgaataaa ttttaactac
     3841 tctataatta tttttctttt tagcggtgtg aatagtgcat tacagggttc cggagatgtc
     3901 agaacgcctt taaatgccct gataatagga aatgttctta atattattct tgatccttta
     3961 ctgattatgg gatatggacc atttccggca atgggagtat caggttccgc agtagcaaca
     4021 gtaataacca gaggaattgg tcttatatat ctttttggtc acgttctttt tggccattct
     4081 actattcatt taggaaaaag acattttaaa ccagacccag ccttgatgca aagaattgtg
     4141 agtattggct tttacgcctc gcttcaagta tttattcggg aaatatcatt tctctttcta
     4201 atgcgtttgg ttacgtcata tggcgatgtc acattggcgg cgtatggaat tggttcacga
     4261 atcaggtctt tcattatggt accgggtttt ggatttgcca gcgcggcggc ggtcctggtg
     4321 ggtcaaaatc ttggggcagg aaaaccagag cgggctaaaa aatcagcttg gcgtgcggtt
     4381 atatattacg aaactattgc ggtcccagta gcattaattt ttctgatctt tgcgcccacg
     4441 attgtaggga tttttaatga tcatcctgaa gtcataacga ttggcagttc cttcttacgt
     4501 tatttggcag ttacttttcc ttttttggca ttttcgctgg tcttttcaca agctttgaac
     4561 ggcgccggtg ataccagaac tgctaccatt gtgaacgcca ttggccagct cggttttcgg
     4621 atacctttgg cttatttctt tgctcgtacg gttggattag gttatacagg tgtctggctg
     4681 ggtattaatg cctctgatgt tgttcaaggt attggaatga tacttgtctt taatgctgga
     4741 cattggcaga aggtattttt aaagcataaa aagaaattag cttacgattt agaaccaaga
     4801 gaaattatca cagagttgcc tacaaattag tgcaaatagg aattttcaga attcagtaat
     4861 cgttatctag cttaatgaaa attttggtga gataaattga aaattaattt tcagatattt
     4921 tgaattgtaa tttctttgaa agaatcctaa atcattataa aaaaatgggt cgcaagctaa
     4981 aatcataaaa aattagatac gattcaataa agggataacg ttaatatgat aatcaaaatg
     5041 gtcttggtag gtattttcac aggtatagta actggcttga ccggtgcatc aggtgtgatg
     5101 gtggtggtgc cattattgag tatactaacg gatttctctg tccatgaagc tattgggaca
     5161 agtttgatag ttgatgttat agctcccttg gcaatatctt atgtttattt taagcataaa
     5221 aatatagata taaaatccgg aatatggata gccgtcgggt caattatcgg agcccaatta
     5281 ggcgcaagtt ttgccggtgg aatatcagag gtaggtttag gcagtacatt tggaattttt
     5341 atggtgatta tgggcattct tatctggaaa aaaggactaa atcacgagtc tttagcaaaa
     5401 gtaatgaaaa aggtcgcaaa atttgaaacc caaactcaaa gaattgtgac tgctctaatt
     5461 ttaggatttt tagttggtat tatgacagga gtattagggg cagggggtgg aggtatgata
     5521 ttattaattt taatattcgt tctaaatttt cctattcata tagcaattgg gacctcagct
     5581 ctaataatgg cgataacggc ttgttctgga gcgatagggt atgctttaca tggtaataca
     5641 aagccagttg agggattaat attgggcatt agcgcggcgg gtagtggaat gttcagcgct
     5701 aagttcgcta acaaagccaa cgaacagact ttaggtaaga tcattggggt aatattttta
     5761 ctacttggga taataatgac tattttaaat tttagataat gttg
//
LOCUS       DAOMJU010000106         5780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_14854_length_5780_cov_18.721048, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000106 DAOMJU010000000
VERSION     DAOMJU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14854_length_5780_cov_18.721048"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1521)
                     /gene="amrB"
                     /locus_tag="PLO58_08640"
     CDS             complement(16..1521)
                     /gene="amrB"
                     /locus_tag="PLO58_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HQO74895.1"
                     /translation="MKKGVIIMTLILSMFTNITSAQERPAYNAGAFYPANPAEITSLI
                     TAYLKNAGAKTLPQKPFGIIVPHAGYIYSGPVAAYAYRELKDQKYDAVIVMSPCHVEY
                     FSFAAIYPGDAYLTPLGKVAVDKKLAAQCVTADRLVKLATEGHLSQRFGRAEHSLEVQ
                     LPFLQIVLPNTPIVPIVIGTHDWRVLESLGNRLGELLVQKNILIVASSDFSHYHSYSE
                     CQKIDQRAIEFLKQLDAVKFYQGFTSETFEACGGAPITALLIAAQKMKNPQIEILKSA
                     NSGDVPHGDKSAVVGYVAVGLYQGTKAKSQDDNTASSQLNTGDLTREEQLYLLDLAEK
                     TVAAAVNHRPLPDPKDVPERLKVKRGAFVTLEKHHELRGCIGYILPIYPLYKTVIEMA
                     KAAALDDPRFPPVSPSELKDITVEISVLTVPQKISDPSEIEVGKHGIIIKRGFNQGLL
                     LPQVATDYGWDRETFLEHTCLKAGLPRDAWQDKNTEIQIFSAQVFNRETVK"
     gene            complement(1524..2651)
                     /gene="amrS"
                     /locus_tag="PLO58_08645"
     CDS             complement(1524..2651)
                     /gene="amrS"
                     /locus_tag="PLO58_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HQO74896.1"
                     /translation="MAITRRQFCLKLGSGVAASVFVPRLIKSGWTKPATDSKPYLREA
                     RYYTPLADRKVRCLLCPRTCLVADGKRGYCRVRENQKGSYKTLVYGRLCSINNDPIEK
                     KPLFHFLPGTMALSVATAGCNLKCKFCQNWNISQVAPEDIPFEYYSPERLVNLAQQIQ
                     SPTIAFTYTEPTISTEYILDTAKLARSKGIHSVMISNGYINSEPLGDLCQVLSAIKID
                     FKAFSDDFYRNVTDGTLQPVLDTMIQIKKNNVWLEIVNLVIPTQNDAPAQFRQMSQWI
                     LQNLGSDIPVHFTRFHPLYLMENLPPTPLRTLESAYEIARECGLRFVYVGNVPGHAAE
                     NTYCPHCNRKIIERIGYQIRTFAIKNGKCQYCQTNIPGFWS"
     assembly_gap    2864..2963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2964..4465)
                     /locus_tag="PLO58_08650"
     CDS             complement(<2964..4465)
                     /locus_tag="PLO58_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQO74897.1"
                     /translation="MKTKSLTWRKISQVFFFLLFTLTLLQATYPFKPWLPPELFLWFD
                     PLAAITTQIAGRFFDIVFLLAAIILISPLIFGRAFCGWICPMGTLIDVSDHILKPKKR
                     RDFRHLRFLKLAILTAVIVLAIFGVQYAWLLDPLPVAWRSIGVIGLAFFFLLSDSILS
                     GLISIGIFPDALLSFQDKIAGFLFPVASPNFGGLVAPLIIFLIILGLSRLSRRFWCRY
                     LCPLGALLGLVARISPLRRIVDADKCSACGICRHKCKMDAIEPDFVTTTKSECILCLN
                     CKYDCATEAISFGWRAPTQMMNKVDLSRRAFIGTSLTALAGVGFLKINKVNPRHSDRL
                     IRPPGALNEDDFLERCIRCGECVRICSTSGKCLQFSVLESGLEGFMTPLAEYRRGYCE
                     YNCNLCGQICPTKAIQPLNVSAKQKAKMGQAIFLKDRCIPYRLNENCIVCEEHCPIPD
                     KAIKINTREYEDPVTGRKRMVSYPYVDEALCIGCGICENKCPLNGEAGIIV"
     gene            complement(4462..5361)
                     /locus_tag="PLO58_08655"
     CDS             complement(4462..5361)
                     /locus_tag="PLO58_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQO74898.1"
                     /translation="MDRRKFIKTSAFLTGGIFLKGNQILGKETTIPDLVMVKDGQPAE
                     MAQKTFELLGGVPRFVAKGDYVLLKPNLSWDRAPEYAATTNPELVAEIVRLCFQAGAK
                     KVVILDNTCADARRSYDACQIPVYARQAGADVRFVRENQFVETPIPNGTNLKSWPIHQ
                     EVFNADKLISIPILKHHGIPGITSGFKNMMGLLGGQRGRIHRPFSENIVDINRVIKPN
                     LTIIDAIRVLRRNGPQGGNLNDVEQMNILLAGTDRVLVDAWAAKIFGLNPLTVEYLKL
                     AHSAGLGEIDTEKFKPVVYSFKA"
BASE COUNT         1579 a         1246 c         1319 g         1536 t
ORIGIN      
        1 ctaattaatt gtttgttact tcaccgtctc acggttgaat acctgagccg agaaaatttg
       61 aatttcggta tttttatcct gccaggcgtc gcgcggtaag ccggctttca gacaagtatg
      121 ttcaagaaag gtctcgcgat cccagccgta atccgtagct acctgcggta ggagcaagcc
      181 ttgattgaag ccacgtttaa taattatgcc gtgtttgccg acttcgattt ccgaggggtc
      241 gctaattttt tgcggaacgg tcaggacgga aatctccaca gtgatgtctt ttaattcact
      301 cggtgagacg ggtggaaagc gcggatcatc cagggcggcc gctttagcca tctcgattac
      361 ggttttatag agcgggtaaa tcggcagaat ataaccgata caacctcgca gttcgtgatg
      421 cttttcgaga gtaacaaaag cgccgcgttt gactttcagg cgttccggaa cgtctttggg
      481 atccggcaga ggtctgtgat taacagcggc ggcgacggtt ttctcagcca gatcgagcaa
      541 ataaagctgt tcctcccgcg tcaaatcgcc tgtattcaat tgactgctcg ctgtattgtc
      601 atcttggctc ttagctttgg tgccttgata aagtccgacc gcaacatagc cgacgaccgc
      661 cgatttgtcg ccgtggggaa catcgccgct attggcagat tttaggattt cgatctgcgg
      721 atttttcatt ttctgagcgg caattagcaa agcggtaatc ggcgcgcccc cgcaagcttc
      781 gaatgtttca gacgtaaagc cttggtagaa tttcacggcg tctaactgct tgagaaattc
      841 aatcgcgcgc tgatcaattt tctggcactc gctatacgaa tgataatgcg aaaaatcact
      901 ggatgccaca atcaggatgt ttttctgtac aagcagttcg cccagccgat tgccgagaga
      961 ttcaagcact cgccaatcgt gagttccaat aacaatcggt acgatcggag tattgggtaa
     1021 aacgatttgc agaaaaggca gttggacttc cagactgtgt tcggcgcgtc cgaatctttg
     1081 gcttaaatgt ccctcagttg ctaatttaac cagcctatcg gcagttacgc actgggcggc
     1141 gagtttctta tctacagcga cttttcctaa cggagttagg taagcgtcgc cgggataaat
     1201 agccgcaaat gaaaagtact cgacgtggca gggcgacata acgattacgg catcatattt
     1261 ctggtcttta agttcacggt aggcatacgc cgcaaccggt ccgctataga tataaccggc
     1321 gtgcgggaca attatgccga acggcttttg cggcaaagtt ttcgccccgg cattcttcag
     1381 gtaagccgtt attagagagg tgatttcagc cggattagcc gggtaaaatg caccagcgtt
     1441 atatgctggt cgttcctgag cagacgtgat gtttgtaaac attgataaaa ttagtgtcat
     1501 aataattact ccttttttca ttgtcaactc caaaatccgg ggatatttgt ttgacaatat
     1561 tggcatttgc cattcttaat cgcaaaagtg cgaatctgat aaccgattcg ttcgatgatt
     1621 ttgcgattgc aatgcgggca ataggtattt tccgcggcat gtcccggaac attgccgacg
     1681 taaacgaatc gcaagccgca ttcgcgggca atctcataag cggattccag tgtgcgtaag
     1741 ggcgtcggcg gtagattttc cattaaatat aagggatgaa agcgagtgaa gtgcactggt
     1801 atgtccgaac caaggttttg caaaatccac tgactcatct gccgaaactg agcaggcgca
     1861 tcgttctggg ttggaattac cagattgaca atctctaacc agacattgtt ctttttaatc
     1921 tgaatcatcg tatccagcac cggttgaaga gtaccgtcag taacattgcg ataaaagtcg
     1981 tcggaaaatg ctttgaaatc aattttgata gctgagagca cttggcataa atcgccaagc
     2041 ggttcggagt tgatgtagcc attagaaatc ataacggaat gaatgccttt agaccgcgct
     2101 aatttagcag tatccaagat atattcggtt gaaatcgtcg gttcagtgta agtgaaagcg
     2161 atggtaggag attggatttg ctgagccaga ttgaccagtc tctcgggtga ataatattca
     2221 aacggaatgt cttcaggtgc tacttgcgaa atattccagt tctggcagaa tttacatttc
     2281 aaattacaac cggcggtggc gacggagaga gccatagtgc ctggcagaaa atggaaaagc
     2341 ggcttctttt cgatcggatc gttgttaatc gagcaaagtc gcccgtaaac cagagtctta
     2401 tagctgcctt tttgattttc acgaacccgg caataaccgc gtttaccatc ggcaaccaaa
     2461 caggtgcgcg ggcagagtaa acagcggact tttcggtcgg caagaggagt gtaatagcga
     2521 gcttcacgga ggtaaggttt ggaatcagtc gctggtttag tccagccgct tttgattaga
     2581 cggggaacga aaacggaagc ggctacccca cttccaagtt tcaagcaaaa ttgacggcgt
     2641 gtgattgcca taacgctaca tcagtttgtt accaatcgaa tgccaacgct gttaaataac
     2701 gatttttatt gttcaaatta cgcatcgcgt tgacctatcc gaatttattt ctatgataat
     2761 ataaaacaaa attgggacaa tgggtatttt catttttatt cagattggca atgattgata
     2821 atgcggttgg cataaatttt tttcaaatca ttttgattcg cgcnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnacgatta tgccagcttc gccattgagt gggcatttat
     3001 tttcgcaaat gccgcaaccg atacagagag cttcatctac atacggataa ctgaccatac
     3061 gcttccgccc ggtgaccgga tcttcgtatt cgcgtgtatt gattttgatg gctttgtcgg
     3121 gaatcggaca atgttcctcg caaacgatgc aattttcatt caatcggtaa ggaatgcaac
     3181 ggtctttcag gaaaattgcc tgtcccattt tggctttttg ctttgccgaa acgtttaatg
     3241 gctggatggc tttggttgga caaatttgtc cgcagagatt gcaattgtat tcgcaatagc
     3301 cgcgccggta ttcggctaat ggtgtcataa aaccttccaa tccactttct aatacggaaa
     3361 attgcaaaca ttttccagaa gtagagcaaa tgcgaacgca ttcgccgcaa cggatacagc
     3421 gttccagaaa atcatcctcg ttcaaagcgc ccgggggacg aattaagcgg tcagaatgac
     3481 gcgggttaac tttattaatt tttaaaaagc cgacgccagc cagagcagtc aaacttgtac
     3541 cgatgaatgc gcgccgcgaa aggtcaactt tattcatcat ctgggtaggt gcccgccagc
     3601 cgaagctgat cgcctcggtc gcacagtcat atttgcaatt caggcagaga atacattcgg
     3661 atttagtagt cgtcacaaaa tcgggttcaa tggcgtccat tttacactta tgtcggcaaa
     3721 tgccacaagc gctacatttg tcggcatcaa caatccgacg gagagggctg attcgcgcca
     3781 ccagacccag caaagctcct aaaggacaga ggtagcggca ccaaaagcgc cgactcaaac
     3841 ggcttaagcc cagtattata agaaaaataa tcaacggggc aaccaatcca ccgaaatttg
     3901 gactcgctac cggaaagaga aatcctgcta ttttgtcctg aaaacttagc agagcatccg
     3961 gaaaaattcc aatactgatt aaaccactta agatcgagtc acttaacagg aagaaaaaag
     4021 ccagaccgat tacaccaata gagcgccagg cgaccggcaa cgggtctaat agccaggcat
     4081 attgcacacc gaaaatcgcc aatacgatca cggcggtgag aattgccaat ttcaaaaagc
     4141 gcagatggcg aaaatcgcgc ctttttttcg gcttcaggat atgatcactg acatctataa
     4201 gagttcccat cgggcaaatc cagccgcaga aagcacgtcc gaaaatcaat ggactgatta
     4261 agataattgc cgctaataga aacacaatat cgaagaagcg accggctatt tgagtggtaa
     4321 ttgccgccaa tgggtcaaac cagagaaaca gctccggcgg caaccagggt ttgaaaggat
     4381 aagttgcctg aagcagggtt agcgtaaata gcaggaagaa aaagacctgc gatattttgc
     4441 gccacgtaag cgatttggtt ttcatgcttt gaaagaatag acgaccggtt tgaatttttc
     4501 agtatcaatc tcacccaaac cggctgaatg tgccagcttc aaatattcta cggtaagtgg
     4561 attaagtccg aatattttcg ccgcccaggc atccaccaag acccgatccg ttccagccag
     4621 caaaatgttc atttgttcaa catcgttcag attaccgcct tgtggtccat tccgtcgtag
     4681 aacacggata gcatcaatga tggttaagtt aggtttgatt acccggttaa tatcaacgat
     4741 attttccgaa aatggtcggt gaattcgccc ccgctgaccg ccgagtaagc ccatcatatt
     4801 cttaaaaccg gaggtaattc cggggattcc atggtgtttc agaatgggga tgctgatcag
     4861 tttatcggca ttaaaaactt cttgatgaat tggccagctt tttaaattgg ttccgttcgg
     4921 aatcggtgtt tcgacaaatt gattctcgcg cacgaagcgt acgtcggcac ctgcctgccg
     4981 agcatatacc gggatttggc aggcatcgta actgcgtcgt gcatcggcac aggtattatc
     5041 gagaattacg acttttttgg caccagcttg gaaacataga cggacgattt cagctaccag
     5101 ctcaggatta gttgtagcgg catattcggg agcgcgatcc cagctcaggt taggttttaa
     5161 tagaacgtag tcgcctttgg caacgaagcg gggaacaccg ccgagaagtt cgaaggtttt
     5221 ctgtgccatt tcagccggtt gaccgtcctt aaccattact aaatcgggaa ttgttgtttc
     5281 tttaccgaga atctgattcc cttttagaaa aattcctccg gttaggaagg cgctagtttt
     5341 gataaatttt cttcgatcca tattaattac ctctaaacct aaaataatat ttatctcgct
     5401 tgctttcaag aaaaaaaaat taaaagcatt ttcatataaa cttgtattca tgcatttctt
     5461 tcaatacagg atgatattcc ccagccattg taacggctta cagaagatcc tgtttgcaaa
     5521 gcgtttctat tatttatatc ggaaaatcag atactaaaaa attaaaaaat tttaggtgag
     5581 agttaatttt tctcttgact tcttaaaacg tttcaattaa tattgaacag atgcgaatta
     5641 tggattacga gattcgaaat caaaccagtt caaagtcaga aagcccggtt ctcaactttg
     5701 gttcaaaagc gtaatttgac aaagaacgca gttgcctttc ataaattata tctttaaaat
     5761 tgagaaggta attgataaga
//
LOCUS       DAOMJU010000107         5674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_15250_length_5674_cov_25.522335, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000107 DAOMJU010000000
VERSION     DAOMJU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15250_length_5674_cov_25.522335"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1400
                     /locus_tag="PLO58_08660"
     CDS             120..1400
                     /locus_tag="PLO58_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74899.1"
                     /translation="MKEINFKINLENEYHNFNQFEAKLIKGIREKVCEQIVQFLEEWD
                     QELGTRLRQHYPQYRHKGYIHRQVRCYFGVIGIKMRRYVHKGWPDIYPLLFILPEDGI
                     SGELMDLLLELVTDVPYQRTNRYIKKATGVAISSQTIWRKVKRLGQDERDRQEQIRCK
                     IFEEGGDDYCQDYRQLPACQSGGKESDLVSPVYIELDGTMVSSREAGEERFEIKSGIM
                     YSQVVQMGEKRRRLMDKVAYGSVSDSETFGENFYAFCRENGLDLRREILFLSDGAQWL
                     NSVAEDVFPEAQKRLDLYHLKKACGIVLDEQELNTLSQAVYYKTGEEIIRLVQNLCSR
                     KNLSGKSVDELMQYLGNNQKAIEYPPGERHGSGGIEKNIGILVGRRFKRQGMSLTAIR
                     QVWSHEGANNLLALRAKKLNQIWQQESQALNYTR"
     gene            1507..2688
                     /locus_tag="PLO58_08665"
     CDS             1507..2688
                     /locus_tag="PLO58_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQO74900.1"
                     /translation="MDLFKKCYAYKDAQIARENGYYPYFIPFSGGDDTVMIYKGQPKI
                     MIGSNNYLGLTHHPKVVEAAQKALNEYGTGRTGSRFLNGTLDIHEEFERELAEFTNKE
                     AALIFSTGYLVNMGVISSLLHKKDYAIIDRLNHASIVDGCRFSGANTVRYRHNDMSDL
                     AKVLKNVPDNSGKLVIVDGIFSMEGDIANLPEIVQIAKASGARIMVDDAHSLGVLGPN
                     GNGTAAHFGLSDQVDLIMGTFSKSFGAVGGFVSGEFEVIDYIKHIARSMIFTASLPPS
                     VVASTRAALQIIKSEPERRQRLIDIGNYMRREFKRLGFNTSTSETPVIPIVIGDDMKT
                     FTFWKMLFEAGVYTNAVVSPAVPSNSSRLRTSYIATHTQEQLDFVLDKFAQIGKQLAI
                     I"
     gene            2757..>3535
                     /locus_tag="PLO58_08670"
     CDS             2757..>3535
                     /locus_tag="PLO58_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQO74901.1"
                     /translation="MSVLKLMITFTRQIKWASLIAICAVLIGCAAQGGPGGGPVDRTG
                     PVLLASFPQDGATNVDLNSDLILNFSEPIDARITDGILQVTPNLSTAPLVKVKHRKVI
                     VKLNEPLQPDRTYIFNFGRNLKDYQNNATAQEIKIAFATGDSIDQGMIAGVVTDIPTN
                     TKTEVWFYKKNTVFPDSLWLAEPDYIISVDKDGNYQATNLPVGEYRALAVSGERLRPK
                     FVSENDLLALPQTEPLIIKNRHDYLENINFRLSKRYLKPFRL"
     assembly_gap    3536..3635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3636..4205
                     /locus_tag="PLO58_08675"
     CDS             <3636..4205
                     /locus_tag="PLO58_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQO74902.1"
                     /translation="QINLSEPVYQDTLAQNIALFYYDTVNVAIHSSWIDANSFIIEPQ
                     KPLISASNYLLQINSRNWCDYAGNFFQDSLFILKFSTIDQNLFGSISGKIISRDGGNL
                     KNLIIDAQLTGSDKFFRQTRPDSTGFYEFIDLLPGKYKFAIWEDRNCNGKYDFGRLAP
                     YLPAEPYRTYQDEINVRSRWETAEVNWSY"
     gene            4356..5423
                     /locus_tag="PLO58_08680"
     CDS             4356..5423
                     /locus_tag="PLO58_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HQO74903.1"
                     /translation="MYSVIMAGGIGKRFWPRSRRERPKQLLDVIGETTMLQMTYERLK
                     SVSDEKKIFIITAQNMKESILKTLKGFPEKNIICEPYGKNTAPAIGLATALIVNKDPE
                     AVIGVFPADHYISNIDEFKKSIGIGFRFARDHTALVTFGIKPTRPATGYGYIQVDKNV
                     KVIEDSVYKVKTFAEKPNLSTAERFLESGEFLWNSGMFIWKGINILNAIKNFIPELYA
                     CLQSIAKSIDTPEYESTLKRHWAMIQSISIDYGILEKAKNVYVVQGNFDWSDVGSWDA
                     VYDLKTKDKNGNVLIGNVATLDTHGCYFYSRKNLLAAVGVSDLIIVQSKDSILVVNRG
                     DSERVKDLVEMLAREGFADHL"
     gene            5429..>5674
                     /locus_tag="PLO58_08685"
     CDS             5429..>5674
                     /locus_tag="PLO58_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74904.1"
                     /translation="MPKKLSNKRLLELRTECEHLLERLGYIVVYGLGNFKEGACLVQS
                     EKKIVINQYTPLDLQVDFLLKILKSQDLSGVFILPALR"
BASE COUNT         1639 a         1086 c         1257 g         1592 t
ORIGIN      
        1 acagaagcat ctcaaaaagt gtggcaaaag acaaaaaaat ggctcacctt cctgtctgac
       61 ctgtctaccc gacaggtgga tagatgttgt ttaaaaacct agaaaaggaa aggcaagcca
      121 tgaaagagat taatttcaag attaatttag agaatgagta tcacaatttc aatcagtttg
      181 aggcgaaatt gattaaggga attcgggaga aggtatgtga gcaaatagta caatttttag
      241 aagagtggga tcaggagtta gggacacgtt tgagacagca ttatcctcaa tatcgtcata
      301 agggctatat tcatcgtcag gtacgttgtt attttggggt aattgggatt aagatgagac
      361 gttatgttca taaaggttgg ccggatatat atccattatt gtttatttta ccggaggatg
      421 gaattagtgg agagctaatg gacttgctgt tggagttggt cacagatgta ccttaccaga
      481 ggactaatcg ctatatcaag aaagccacag gggtagcgat cagttcgcaa acgatctggc
      541 gtaaagtaaa gcgcttaggt caggatgaac gtgatcgaca ggagcaaata cggtgcaaga
      601 tatttgaaga gggtggtgat gattattgtc aggattatcg tcagttacct gcctgccagt
      661 caggcggcaa agagtcagat ttagttagtc cggtctacat agaactggat gggacgatgg
      721 ttagtagtcg tgaggcagga gaagaacgtt ttgagattaa gagcggcata atgtatagcc
      781 aggtagttca gatgggggag aagcgtcgtc gtttaatgga taaagtagct tatggtagtg
      841 ttagtgatag tgagaccttt ggtgagaatt tttatgcctt ttgtcgtgag aatggactgg
      901 atttacgccg ggagatattg tttctgagtg atggtgctca atggttaaat agtgttgctg
      961 aggatgtgtt tccggaagct caaaagcggc tggatttata ccatctaaag aaagcctgtg
     1021 gtattgtttt agatgagcag gaattaaata ctttgagcca ggcagtttat tataaaactg
     1081 gcgaggaaat cattaggtta gttcagaatt tatgtagtcg gaagaatcta tctgggaagt
     1141 cagtagatga actgatgcaa tatttaggga ataatcaaaa agccattgaa tatccaccag
     1201 gggagcgtca tggctctggt ggaattgaaa agaatattgg tattttggtt ggacgacgct
     1261 ttaaaagaca aggtatgtcc ctgactgcca ttaggcaagt gtggtctcac gaaggcgcta
     1321 acaatcttct cgccctaaga gcaaaaaaat taaatcagat atggcaacaa gaatcacaag
     1381 ctttaaatta taccaggtga gcgccacact atttgttatg cttccctcag ttatttatca
     1441 atttgcaaat caatatatcc cactttaaat ttgcaacgca tattgaaatt tattacggtg
     1501 tgaacaatgg atttatttaa aaagtgctac gcttataaag atgctcagat tgcgcgtgaa
     1561 aatggatatt atccctattt tataccattt tcgggtggtg acgacacggt gatgatttat
     1621 aaaggtcagc ccaaaataat gattggttct aataattact taggactgac tcatcatcca
     1681 aaagtcgtcg aagccgctca gaaggcactc aacgaatatg gcaccggccg caccggtagt
     1741 cggtttttga acggaactct ggacattcat gaggaattcg agcgggaact ggctgagttc
     1801 accaacaaag aagccgctct gatattttca accggttatc tggttaatat gggagttatt
     1861 tcaagtctcc tacataaaaa agattatgct attattgatc ggcttaatca cgcgagcatc
     1921 gtggatggtt gccgtttctc cggggctaat accgtccgtt atcgccataa cgatatgagc
     1981 gatctggcta aagtcttgaa aaacgtgcca gacaatagcg gaaaattagt aattgtggac
     2041 ggcatctttt caatggaagg cgatatcgcc aacttgcccg aaattgtgca gattgctaaa
     2101 gcttccgggg ctagaattat ggttgatgac gcccactctt tgggtgttct gggacctaat
     2161 ggtaatggca ctgcggctca ctttggtttg agtgatcagg ttgatttgat tatgggcact
     2221 ttctctaagt cattcggcgc tgtcggcggc tttgtgtccg gcgagtttga agttatagat
     2281 tacatcaaac acatcgcccg gagtatgatt tttaccgcca gtctgccgcc gagtgtggtg
     2341 gcatctaccc gggccgctct ccagatcatc aaatctgaac ccgaacgccg tcagcgatta
     2401 attgatattg gtaattatat gcgccgagaa tttaagcgtt tgggatttaa cacctcgact
     2461 agcgaaaccc cggtgattcc aattgtcatt ggtgacgata tgaaaacttt cacattctgg
     2521 aaaatgttat tcgaagccgg tgtttatacc aatgccgttg tcagtccggc agttcccagc
     2581 aactcttcgc gccttcggac cagctatatc gccactcata cccaggaaca gctggatttt
     2641 gtgttggaca aatttgccca aataggtaaa caattagcca ttatttagtg acaattaaac
     2701 cggttaaacc gaaccggaac gattccctac atttctgttc taataaactt agtataatgt
     2761 cagttctcaa attaatgata acctttacac gccagataaa atgggcatct ctgatagcga
     2821 tttgtgcggt tcttatcggg tgtgcggctc agggcggtcc tggtggggga ccagtcgatc
     2881 gcaccggtcc ggtgcttttg gcaagttttc cacaagatgg cgctaccaac gttgatctta
     2941 actctgatct catcttgaat ttttctgagc caattgatgc acgtataact gatgggattt
     3001 tgcaagtaac tccgaacctc tcaactgcgc cccttgtaaa agtcaaacac cgtaaggtga
     3061 tcgtcaaact aaacgagccg ttacaaccag atcgcaccta catcttcaat tttgggcgca
     3121 atctgaaaga ttaccagaac aatgccaccg ctcaggaaat caaaattgcc tttgccaccg
     3181 gcgactcgat tgaccaagga atgatagctg gagtcgtgac tgacattcca actaatacca
     3241 aaaccgaggt ttggttctat aaaaaaaata cggttttccc cgattccctc tggcttgctg
     3301 agccggatta tattatttcg gtggataaag atggcaatta tcaggcgact aatttaccgg
     3361 tcggtgaata tcgcgctctg gccgtttccg gtgaaagact ccgtcctaaa ttcgtctccg
     3421 aaaacgatct gctggcactg ccccaaactg agccgcttat tattaaaaac cggcacgatt
     3481 atcttgaaaa tatcaatttt cgcttatcaa aaaggtattt gaaaccgttc cggctnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncaaat caacctgagt gaaccggttt
     3661 atcaagatac tttagcgcag aacattgccc ttttttacta cgatacagta aacgttgcaa
     3721 tccattcatc ctggattgac gccaattctt ttatcattga accgcagaag ccactaattt
     3781 cggcttccaa ttatttgtta cagatcaatt cccgaaattg gtgcgactat gccggtaatt
     3841 tttttcagga ttctttattc atcttgaaat tcagtacaat agatcaaaat ttgttcggca
     3901 gtatttcggg taaaattatt tccagagatg gcggtaatct caaaaatctg attattgatg
     3961 ctcaactcac cggctctgat aaatttttcc gccagactcg gcctgacagt accggatttt
     4021 acgaattcat agatttacta cctggaaaat ataaatttgc tatctgggaa gaccgcaatt
     4081 gcaatggaaa atacgacttc gggcgcttag caccctatct cccggcggaa ccgtatcgaa
     4141 catatcaaga cgaaattaat gtacgttccc gctgggaaac agccgaagtt aattggagtt
     4201 actaatgact gcggtgaatt atcagaaaag attaaaggct gataacatcc tgttatattc
     4261 tttagcagac tcacctttat tttataaaaa ttcagagtgg atttccttgc gcagtctctt
     4321 gtgaaatttt ttagtttaaa taggagtatt caaaaatgta ttccgtaatt atggcaggtg
     4381 gaattggtaa gcgtttctgg cctcgcagtc gccgcgaacg tccgaagcaa ttattagatg
     4441 tcattggtga aaccactatg cttcagatga cttatgaacg attgaaaagt gtttcagatg
     4501 agaagaaaat ctttatcatt actgcccaaa atatgaaaga gagcatttta aaaaccttaa
     4561 aaggctttcc ggagaaaaac ataatatgtg aaccttatgg taaaaatacg gcgccagcta
     4621 tcggtctggc tacagcttta atcgttaata aagacccgga agcagttatt ggcgtttttc
     4681 cagctgatca ttatatcagc aatatcgacg agtttaaaaa atcaatcggt atcggttttc
     4741 gattcgccag ggatcatacc gctcttgtaa cctttggaat taaaccgact cgtccggcaa
     4801 ccggctacgg ttatatccaa gttgataaaa acgtcaaagt tatcgaagat tcagtttata
     4861 aggtgaaaac ctttgccgaa aaacccaatc tatctacagc cgaacgattt ctggaaagcg
     4921 gcgaatttct ctggaacagc ggtatgttca tctggaaagg catcaatatt ttaaatgcca
     4981 taaaaaactt catccccgaa ttatacgcct gcttacaatc catcgctaaa tctatcgaca
     5041 cgcccgaata tgaatccact ctgaaacgcc attgggcgat gattcagtca atttcaattg
     5101 attatggaat tctggaaaaa gccaagaacg tctacgttgt tcagggaaat ttcgactgga
     5161 gcgatgtcgg ttcatgggac gctgtatatg acttaaagac caaagacaag aacggaaatg
     5221 ttttgattgg caacgtagct actctggata cccatggttg ttatttctat tcgcggaaaa
     5281 atttgttagc ggccgtcgga gtgagcgatt taatcatcgt gcaatctaaa gattccattc
     5341 tggtcgtaaa tcgcggtgac tcagagagag tcaaagactt agtcgaaatg ctggcgcggg
     5401 aaggctttgc tgatcacctg taaataatat gcctaagaaa ttatccaata aacgtctcct
     5461 ggaattacga actgaatgcg aacatttact cgaacgcctc ggttatatcg ttgtttacgg
     5521 actgggaaac tttaaggaag gcgcttgtct ggtgcagagt gaaaagaaaa ttgtaatcaa
     5581 ccaatacact ccgttggatt tgcaggttga ctttttacta aaaatactaa aaagtcagga
     5641 tttatccggc gttttcattt taccggcttt gcgc
//
LOCUS       DAOMJU010000108         5590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_15558_length_5590_cov_21.836134, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000108 DAOMJU010000000
VERSION     DAOMJU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15558_length_5590_cov_21.836134"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLO58_08690"
     CDS             <1..429
                     /locus_tag="PLO58_08690"
                     /inference="COORDINATES: protein motif:HMM:NF040055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HQO74905.1"
                     /translation="NPNATIMLVENAERFGLTQMHQLRGRIGRGRKNGVCILVERKVT
                     ELGHQRIKTILSTIDGFEISEADLKLRGPGEFYGTRQHGYLKMKIADPLTDIELLKTA
                     RVRAFTLLKSDPHLRRGENAATRNHLLQQYANQIDFSNIL"
     gene            438..749
                     /locus_tag="PLO58_08695"
     CDS             438..749
                     /locus_tag="PLO58_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HQO74906.1"
                     /translation="MPEKQPDRQQILFLSLVQSLVSSAWIHLGKMKNPVTGTNSIELE
                     EASVSIDMLEMLHNRTQGNLSDDEKRFLENALSDLKINFVEAKLHQSAASEKPNEESH
                     S"
     gene            776..3676
                     /locus_tag="PLO58_08700"
     CDS             776..3676
                     /locus_tag="PLO58_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HQO74907.1"
                     /translation="MNWFKKDIEKKILALLTLLLSFIVYLDTISPTVPFWDCGEFIAT
                     AYTLGVPHPPGSPLFLLIGRIFSMIPFSQDIAFRVNLISTIVSALAVMFLFLITVQLL
                     QFRGKPTTKLQKLIVYGGAFIGAMTFAFTDSHWFNAVEAEVYAFSTFLTAIVVWLILV
                     WEEKAGQPGHERYLLIIAYIVGLAIGVHLLNLLAIFFIALIIYFKQKKVSSVWWLILD
                     IIIGVAVAGLLFLISMQFRVSIFVQAIITLGILVLGYYQLYRKARKPRLKEHAQNVLM
                     AFAASAAFLIIYVGIMKGLPILADKVGLWATVLAIVLLFAVTIWAVRKKHNLLALIMM
                     ALVLIVVGYSTYVTIFIRSAQDPNIDENDPETTHQAVAYLEREQYGQRSFTDIFNRRV
                     WKPEAAHKYSSALDYFWNYQIKYMYIRYFNWQFIGRKGSDVDFFQFILPFPFIIGLFG
                     LFVHFNEDKHKAVAVLALFLFTGLMIVLYLNQDDPQPRERDYSYVGSFFAFSIWIGIG
                     AAALLERISQLKNKKLSRPLTYITAGLLLLALPINVLVANYHEHDRSGNYVASDYAYN
                     ILQSCGPNGILFTNGDNDTFPLWYMQEVEHLRRDVKVVNLSLLNTSWYIKQLRNIEPK
                     INIGNISDATIDNIGPIPWKKQKVRISPPPESLLTSDKYPEFRQFGYNPKEFPPMEWE
                     VEPTLKYGNQGYLRVQDIMILQILEANRWERPLYFASTVSPDNKLGMEDFMSMEGLVF
                     RIYPQKVKRIDADKMRHNLFNVFRYRNLNNPKVYYDDNIKRLMFNYQTAFIQLAIEEL
                     YSGNKERMLATLDTMKYLIPEEIFPIEYDDIYLQIGLLYYDGGRRGEPGQPSELEKRL
                     ETLLNKPNVNYRNKLKYASIYAQVLGDYPKAIQILEELNAQKPNDPEISGYLVKVYEE
                     AQQLDKAVRVLDNWLALYPNDSGAAEMRNLYLSKMTPAQKAQYDSLTKVYHR"
     gene            3688..4728
                     /locus_tag="PLO58_08705"
     CDS             3688..4728
                     /locus_tag="PLO58_08705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQO74908.1"
                     /translation="MSSTPEIAVIIPHYNGVAILRDCLNSLFSSTDLPLDVILVDNAS
                     TDESVKMVRSEFPAVRILPQSQNRGFAGGCNAGIRATEAPFVLILNNDTIHQAGWLEL
                     LLEKIKSQPELVAVQPKILSWQNREQFDYSGAAGGEMDIFGFPFARGRIFETIEMDEG
                     QYDYLPDRIFWASGTAFLARRELLIEAGLFDETFFAHMEEIDLQWRLQLMGYSIGVEP
                     RAVIWHRSGSTLGAEAPLKKYLNHRNSLLMFLANYKPLVTAYLLPIRILLDWMALAFS
                     LLKLDLGRAGAILKAHGWMISHPAVTFKRRRQVKALRRITDFQIMEKLYRGSIALSYY
                     LLKKKSYLDLVD"
     gene            complement(4732..>5590)
                     /locus_tag="PLO58_08710"
     CDS             complement(4732..>5590)
                     /locus_tag="PLO58_08710"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HQO74909.1"
                     /translation="EILMKKCKPIQLAMVLLLFLSQIFAQFVDVKVEISVDRMPELER
                     SDLKVLEMAIPAYYENYEWIENTYGIQIPLKINIYPQSVNTTGSERIFTAQLFIVNES
                     GDMRFFEKNFRFVYNTNDPIVHSEMIQTLTGALDFYAWMMIASEMDTYEQLGGNAYYE
                     KARDVATRGQMSERPAGWKDRLQELDEILRLRNYRLMKYHFWAAMDYADQGNREAVAP
                     EIGKALEAIEAIFAENARERYSHIFLDAHARDIMTIIKEFGTIEQAHKLMALDPDNKP
                     VYEKIIYSD"
BASE COUNT         1493 a         1230 c         1220 g         1647 t
ORIGIN      
        1 aatcccaacg ccactattat gctagtcgaa aatgccgagc gattcggttt gactcagatg
       61 caccaattgc gcggacgaat cggtcgcggt cgcaaaaacg gtgtctgtat tctggtcgaa
      121 cgcaaggtta ccgagctggg tcatcagcgg attaaaacaa ttctctcgac gattgatggc
      181 tttgaaatct ctgaggcaga ccttaaatta cgtggacctg gagaatttta tggcacacga
      241 cagcacggat atttaaaaat gaaaattgcc gacccgttga ctgacattga gttattaaaa
      301 actgctcgcg tgcgcgcctt tacgcttttg aaaagcgatc cccatttacg ccgcggcgag
      361 aatgcggcta ctcgcaatca tttattgcaa caatatgcta atcaaattga tttcagcaat
      421 attttatagg tgacattatg cctgaaaaac aacctgatcg tcagcaaatt cttttcctta
      481 gtttagtgca atcgctggtc agtagtgcct ggattcatct cggtaaaatg aagaatccgg
      541 taaccggaac caatagcata gaactggagg aggcttccgt gagcattgat atgctggaaa
      601 tgctccataa cagaactcaa ggcaatctct ccgatgatga aaagcgcttt ctggaaaacg
      661 ctttgagtga cttaaagatt aatttcgttg aggctaaatt acaccagagt gccgcctcag
      721 agaaaccgaa tgaagaatct cattcataaa acaataagtc tataaaggaa attacatgaa
      781 ctggtttaaa aaagacatcg aaaagaaaat tctcgccttg cttacgcttc tgctcagttt
      841 catcgtctat ctcgatacaa tttcaccgac ggtaccattc tgggattgtg gcgaatttat
      901 tgctaccgcc tatactctcg gagttccgca tcctccgggt agcccgctat tcttgctgat
      961 tggtcgcatt ttttcgatga ttcctttcag ccaggatatt gcgttccggg ttaatttaat
     1021 atccaccatc gtcagtgcat tggcggtgat gtttctcttt ttaattactg ttcagcttct
     1081 gcaatttcgc gggaaaccca cgaccaaatt acaaaaattg attgtctatg gtggagcttt
     1141 tattggcgct atgactttcg ctttcactga cagtcattgg ttcaacgcgg tggaagccga
     1201 agtctatgcc ttttcgacgt ttttaactgc tattgtggtt tggttgattt tggtttggga
     1261 agaaaaagcc ggtcagcccg gacatgagcg ttatctatta atcatcgcct atattgtagg
     1321 tctggcaatc ggtgtccatc tcttaaatct cctggcaatt ttctttatcg ccttgataat
     1381 ttattttaaa caaaagaaag tgtcttcggt ctggtggttg attctggata taattatcgg
     1441 ggtagcggtt gccggtttac ttttccttat ttccatgcaa ttcagagtgt caatatttgt
     1501 tcaggcgata ataacattag gtatcctggt gctgggttat taccagcttt atcggaaagc
     1561 tcggaaacca cgcctgaaag aacatgctca aaatgtcttg atggcttttg cggcctccgc
     1621 cgcctttctg ataatttatg tcggcataat gaagggttta ccaatcctcg ccgataaagt
     1681 tggactctgg gcaaccgttc tggcaattgt tctcctcttt gcggtaacca tctgggcagt
     1741 tagaaaaaaa cataatctcc tggcgttgat aatgatggcg ctcgttctta tcgttgtcgg
     1801 ttattccact tatgtgacca tttttattcg cagtgcccag gatccgaata ttgacgaaaa
     1861 tgatcccgaa actactcatc aggcggtcgc ttacttagag cgtgagcaat atggtcagcg
     1921 tagttttacc gatatattta atcgccgagt ttggaagcca gaagccgctc ataaatattc
     1981 cagcgctttg gactatttct ggaattatca gattaaatat atgtatatcc gctatttcaa
     2041 ctggcaattc atcggacgta agggaagcga tgtagatttc tttcaattta tcttgccatt
     2101 tccgtttatt atcgggcttt ttggtctctt tgtccatttc aatgaggaca agcacaaagc
     2161 cgtggcagta ttggcgcttt tcctatttac gggactgatg atagttttat atcttaatca
     2221 ggatgatccc cagccgcggg agcgagacta ttcttacgtc gggtcattct tcgcattttc
     2281 gatctggatt ggaattggag ccgcggcttt gcttgagcga atttcccaac tgaaaaacaa
     2341 aaaattaagt cgtccactta cctatataac agccggttta ttattattag ccttgccgat
     2401 caatgtttta gtagccaatt atcacgaaca tgatcgaagc ggtaattacg ttgcctcgga
     2461 ttacgcttac aatatcctgc aatcctgtgg gcctaatggt attcttttta ctaacggtga
     2521 caacgatact ttccctctct ggtatatgca ggaggtcgaa catctacgtc gcgacgtcaa
     2581 agtcgtcaat cttagcctgt tgaacacctc ttggtacata aaacaattac gcaacatcga
     2641 accaaaaatc aatatcggaa atatttccga tgcaaccatt gataatatcg gtcctattcc
     2701 ttggaaaaaa cagaaagtaa gaatcagccc gccaccggaa agtttactta cctcagataa
     2761 atatcctgaa tttcgccagt tcggctacaa tcctaaggaa tttccaccga tggagtggga
     2821 agtcgaaccg actctgaaat atggtaatca gggttatctg cgcgtacaag atattatgat
     2881 cttacagatt ctcgaagcta atcgctggga acgaccgctc tactttgcca gtacggtttc
     2941 accggataat aagcttggta tggaagattt tatgtcaatg gaaggattgg tattccgcat
     3001 ttatccccaa aaagtaaaac ggatcgacgc cgataaaatg cgccataacc tatttaatgt
     3061 ttttcgctat cgcaatttga ataatcccaa ggtttattac gatgataata tcaagcgctt
     3121 aatgttcaac tatcagacgg cttttatcca acttgctatc gaagaacttt atagcggcaa
     3181 taaagaacga atgttggcga ccctcgacac tatgaagtat ctaataccag aggaaatttt
     3241 ccccattgaa tacgatgaca tttatctcca gattggtctg ctttattatg atggcggccg
     3301 ccgtggtgaa ccgggacaac ccagcgaact cgaaaaacgc ctggaaaccc tattgaacaa
     3361 acccaatgtg aattaccgca ataaattgaa atatgccagc atttatgccc aggtgctggg
     3421 agattatcct aaggctattc aaatcctgga agaattgaat gctcagaaac caaacgatcc
     3481 ggaaatctcc ggctatttgg tgaaagtcta tgaagaggct caacaattag ataaggcggt
     3541 gagagtactg gataactggc tggcacttta tcctaacgat agcggtgccg ccgaaatgcg
     3601 caatctttat ctgagtaaga tgactccggc tcaaaaggcg caatacgatt cgctgaccaa
     3661 agtctatcac cgctaaacca atccggtatg tcgtccactc cggaaatcgc ggtcattatt
     3721 cctcactata acggtgtggc gattttgcga gattgtctga actcgttgtt ttcctcaacc
     3781 gacttacctc tcgatgtcat actggttgat aacgcctcca ccgacgaaag cgttaaaatg
     3841 gttagaagtg aattcccagc cgtccggatc cttccacaat ctcaaaatcg cggttttgcg
     3901 ggcggctgta atgccggtat tcgcgctacg gaagccccat ttgtcctaat cctgaataat
     3961 gataccattc atcaagccgg gtggttggaa ttattactgg aaaaaatcaa atcacagccc
     4021 gaattagtcg ccgttcagcc taaaattctt tcttggcaaa accgtgagca gtttgattat
     4081 tccggagcgg cgggaggcga gatggatatt ttcggctttc cgtttgcccg cggtcgaatt
     4141 ttcgagacta ttgaaatgga cgaagggcaa tatgattatt tacctgatcg cattttctgg
     4201 gcttccggga cggctttttt agccagacgg gaattattaa tcgaagcggg actttttgat
     4261 gaaactttct tcgcgcatat ggaagaaatt gatctccagt ggcgactgca acttatgggc
     4321 tactctatcg gcgtcgagcc tcgcgctgtc atctggcatc gtagtggttc tactctggga
     4381 gcggaggcgc cgctaaaaaa gtatctaaac catcgtaaca gtttgttgat gttcctggcg
     4441 aattataagc cgctggtgac cgcttacctt ttgccgattc gtattctgct ggactggatg
     4501 gcgctggcat tttccctatt gaaattagat ttaggtcgcg ccggtgccat tctgaaagct
     4561 cacggctgga tgatcagcca cccggcagtc acttttaaac gccgccgcca ggtaaaggct
     4621 ctccgtcgga ttaccgattt ccagataatg gaaaaattat accgtggttc aattgccttg
     4681 agctattatt tactgaagaa aaagtcttat ttggacttgg tagattaaaa attaatccga
     4741 ataaataatt ttttcgtaaa ctggcttatt atccggatca agcgccatca gtttgtgagc
     4801 ttgttcgata gttccaaatt ccttgatgat cgtcattata tcgcgggcat gggcatcgag
     4861 aaaaatatgc gaatatcgtt cgcgggcatt ttcggcaaat atcgcctcga tggcttccaa
     4921 ggctttgcca atttcgggag caaccgcctc tctatttcct tgatcggcat agtccattgc
     4981 cgcccagaaa tgatatttca ttaatcggta gtttcgcagg cggagaatct cgtccaattc
     5041 ttgcaggcgg tctttccaac cggcgggtcg ttcggacatt tgcccgcggg ttgctacgtc
     5101 ccgcgctttc tcgtaatagg cattgccgcc cagctgttcg taggtatcca tttcgctggc
     5161 aatcatcatc caagcataaa agtccagcgc accggtcagg gtttgaatca tttcggaatg
     5221 gactatcgga tcattagtat tatagacaaa cctgaaattt ttctcaaaaa aacgcatatc
     5281 gcctgattca ttgacaatga acaactgggc agtgaaaatc ctttcggaac cggtcgtatt
     5341 gacactttgg ggatagatat tgatttttag tggaatttga atgccataag tgttttcgat
     5401 ccattcgtag ttttcgtaat aggcagggat tgccatttcg aggactttca gatccgagcg
     5461 ttccaattcc ggcatacgat ccaccgatat ttcgactttg acgtccacaa attgagcaaa
     5521 tatttgactt agaaaaagta acaataccat cgccagctgg ataggtttgc attttttcat
     5581 tagaatttcc
//
LOCUS       DAOMJU010000109         5489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_15956_length_5489_cov_16.986014, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000109 DAOMJU010000000
VERSION     DAOMJU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15956_length_5489_cov_16.986014"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..2191
                     /locus_tag="PLO58_08715"
     CDS             122..2191
                     /locus_tag="PLO58_08715"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQO74910.1"
                     /translation="MDRIVQHPILTIPPARKLVKFTFNDTILEGFEGEPVSSALMAQG
                     YREFSIHRKGDTPQGLFCANGQCSHCTLVIDGLAQKSCVTPLKGGMQIKTLRHIPEIP
                     ADNRPLTHYKKIELTCDILVIGGGPAGLMAALELAQLNYNVIVVDDKEKLGGKLLLQT
                     HKFFGSIEDCYAGRRGIEIAALLEKELSSHTNIKIFTDTTVAAIYKDKKAGLFIKNQN
                     YAIVAFRGLIISPGAREKSLIFNGNHLPGVFGAGAFQTLVNRDLVKAGRRIFIVGSGN
                     VGLIAAYHALQAGITVVGICDILDKVSGYKVHADKIERMGVPIYLKHTVIAAEGNGKV
                     ERVIIAAVDDHFQPLLETARTFAVDTLLIAVGLTPLDEFFEMANQFGFPVVKAGDADE
                     IAEASSAMFSGRIAARRLANQLGKNIAIDSTFIQKAEILKNKPGRIFPSTPVVLDEKF
                     QPIIRCTEEIPCNPCTAVCPVNAIQLRGDRHDLMDLPFYQGGCTGCMQCVLICPGLAI
                     TLARKIDEQFGEVVLPHEYLATFKVGDRLELTGVSGEFLESGEILKIRYFKKHKTYLI
                     HIKTTLQNAPLVAGIRVQPESETTALPEAQFKYLPDDGIVCHCELVTVKEIRDFIREH
                     NVYDVNQLKLIRVGMGACGGKNCSVLLSRIFEEAGVDFHTVTPGTKRPLSVEIPMSAI
                     VNEESES"
     gene            2192..>2360
                     /locus_tag="PLO58_08720"
     CDS             2192..>2360
                     /locus_tag="PLO58_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQO74911.1"
                     /translation="METYDVVIIGAGSIGTPLSYYLTRSGLRVIVLEQLASVGRGQNR
                     AAIGGIRATHSD"
     assembly_gap    2361..2460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2461..3101
                     /locus_tag="PLO58_08725"
     CDS             <2461..3101
                     /locus_tag="PLO58_08725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQO74912.1"
                     /translation="EPVTGFKWNQNKITTIITTKSTYSCRWVINAAGAYARRIGEMLE
                     LDLPVFPESHEGGITEPVSRFFAPMIVDVRPDSESSNYYFYQNSEGQVVFCITPEPKI
                     AGTDCDSTSNFLPLVTKRMLSLYPRLRHLRVRRTWRGLYPATPDGSPIVGFTHQFCNL
                     FLAIGMCGQGFMIGPGLGAIIAEILTDNTHKYDEILDQWNLYRNFTGQEILK"
     gene            3118..4314
                     /locus_tag="PLO58_08730"
     CDS             3118..4314
                     /locus_tag="PLO58_08730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQO74913.1"
                     /translation="MTIPISKRAQALKPSPTMSVTALASKMAAEGISIINFGVGEPDF
                     NTPENIKQAAHQALDANFTHYTVSAGILELRQAICDKLKRDNNLDYSPDEIIVAPGGK
                     AAIITTLAAICDPDDEIIIPAPYWVSYPSQVEMLDGKPVFIATDESTNFKITADQLES
                     VIKNLSQPKALILNSPNNPTGAVYSPTELAAIGAVCLKHNLAVISDEIYEKLVYGSAR
                     HYSIVQVLPEIRSITIVINGFSKAYAMTGWRVGYAAGPREVIKRATAIQEHTASCVTS
                     ITQKAAVAALNGDDSSIEAMRQEFERRRDYLVNELKQIPLVDCPLPDGAFYAFPNISH
                     YIKNNKIGIRDSVQLCEYLLKNFHIALVPGSAFGCDDCLRFSYATSMENIVEGVKRFK
                     SGLASL"
     assembly_gap    4315..4414
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4415..5475)
                     /locus_tag="PLO58_08735"
     CDS             complement(<4415..5475)
                     /locus_tag="PLO58_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74914.1"
                     /translation="MRFNLDDLENYVVYDTTGGKLAGSAGYGGRDDYLGIAYMALDNQ
                     NNLWIANQYAQNDSFIAVLTPDDRWVHFSGQESGNYLNYLVTSIAFDAAGRVWFASEA
                     TSEPPGSNGGIAVLDYKNTLFDKSDDQWYRVTTSDGLASNMVYSLAFDLENKLWIMTA
                     GGIQSASVSESFPARVFDAIDYAALTNIAFAKECRIKVDGLNNKWITTVNSGVKLYTY
                     NGIWLNDKEGFTSKNSGLLSDNILDIAFDPPRGLVYISTDKGISVYKSPYAYYGNQYQ
                     KPNTFPSPFRIPADQPLTIDGLLQDSEVKIMLLDGTFIRHLDPKDGEVVGQQAYWDGR
                     NEQGKLVSSGVYIFVAYTP"
BASE COUNT         1450 a         1238 c         1141 g         1460 t
ORIGIN      
        1 aatttattcg taaatttttt aataatattt tctctcatta aatattttca ttaagccacc
       61 gtatatctaa atttgaccaa ttattttatg attacaaatc ctgttaaata caaaggcgaa
      121 aatggacaga atagttcagc atcccatttt gacgattcca cccgctcgaa aactagtaaa
      181 atttactttc aatgatacta ttctggaagg ctttgaaggc gaaccggttt cttcggcgct
      241 gatggcgcag ggctatcggg aattttccat tcaccgcaaa ggtgacacgc cgcagggact
      301 cttctgtgcc aatggtcaat gttcgcattg cactttagtc attgacggtt tggcgcaaaa
      361 atcctgtgtg acgccgttaa aaggcggtat gcagattaaa accctgcgcc atattccgga
      421 aattccagcc gataaccgcc cactgaccca ctacaaaaaa atcgaattga cttgtgatat
      481 attggtaatt ggcggcggac ctgccggatt gatggccgct ctcgagctcg cccaactgaa
      541 ttataacgtt atcgttgttg acgataagga aaaattaggc ggtaaacttc tacttcagac
      601 gcataaattt ttcggctcga ttgaagactg ttatgctggt aggcggggaa ttgaaattgc
      661 cgctttactg gagaaagaat taagcagtca cacgaatatt aaaattttca ctgatactac
      721 tgtagccgca atttacaagg ataaaaaagc cgggcttttc atcaaaaacc aaaactatgc
      781 gattgttgcc tttcgcggct tgataatttc tcccggtgct cgcgaaaagt cactgatttt
      841 caatggcaat caccttccag gagttttcgg tgctggtgct tttcagaccc tggtcaatcg
      901 tgatctggtt aaagctggtc gtcgcatttt cattgttggt agcggcaatg tcggtttgat
      961 tgcggcttat catgctttac aagctgggat cacagttgtc ggcatttgtg acattcttga
     1021 taaagtttcc ggctacaagg ttcacgccga caaaatcgag cggatgggcg tgccaatcta
     1081 cttaaaacac accgtaatcg ccgccgaagg caacggcaaa gtcgagagag ttatcattgc
     1141 cgcggttgac gatcattttc aacctttatt ggaaactgct cgaacttttg cagttgatac
     1201 tttactaatc gcggttggtt taactccact tgatgaattt ttcgagatgg ctaaccaatt
     1261 tggttttccg gttgttaaag ccggtgatgc cgatgaaatc gccgaggctt catcggcgat
     1321 gtttagcggg cgaattgcgg cacggcgtct ggcaaatcag cttggcaaaa atatcgcaat
     1381 agattcgact tttatccaga aggctgaaat tttaaaaaac aagccgggac gaatttttcc
     1441 atcaactccg gtagttttgg atgaaaaatt tcagccgatt atccgttgta cggaagaaat
     1501 tccctgtaat ccttgcacgg cggtttgccc ggtgaatgcc attcaacttc gcggtgatcg
     1561 tcacgattta atggatttac ccttttatca aggcggctgt accggctgta tgcaatgcgt
     1621 tttaatctgt cctggattgg ctattacctt ggctcgtaaa attgacgaac aatttggcga
     1681 agtggtttta ccgcacgaat atttagcgac cttcaaagta ggcgatcggc tggaattgac
     1741 cggagtttcg ggagaatttc tagaaagcgg tgagattctg aaaatacgct attttaaaaa
     1801 acataaaact tacctgatcc acattaaaac caccttacaa aatgcgccgc tggtagccgg
     1861 aatcagagtg caaccagaat ctgagacaac tgcgttgcct gaggcgcaat ttaaatattt
     1921 accggacgac ggaattgttt gccattgtga actggtcacc gtcaaagaaa tccgcgactt
     1981 tatccgcgag cataatgttt acgacgttaa tcaattgaaa ttaatcagag tcggtatggg
     2041 cgcctgcggt ggcaaaaatt gctccgtgct tctatcacgt attttcgaag aagctggggt
     2101 tgatttccac accgttacgc ccggcaccaa gcgcccactc agcgttgaaa ttccaatgtc
     2161 tgccatcgtc aacgaagaaa gcgaatctta aatggaaacc tatgatgtcg ttataatcgg
     2221 agcaggcagt attggtacac cacttagtta ttatctaacg cggagcggtc tgcgtgtaat
     2281 tgtgctggaa caattggctt cagttgggcg cggtcagaat cgcgccgcta tcggaggcat
     2341 tcgggctact cactctgatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 tggaaccggt caccggtttt aagtggaatc aaaataaaat tacgaccatt attactacta
     2521 aatccaccta ttcatgtcgt tgggtaatca atgccgctgg agcctatgct cgtcgcattg
     2581 gcgaaatgct ggaactcgat ttgccggttt tcccggagag ccacgaaggc ggtattaccg
     2641 aaccggttag ccgctttttc gcaccgatga tcgtagatgt ccgaccggac agcgaatcaa
     2701 gcaattatta tttttaccag aatagcgaag gtcaggtggt cttttgcatc acacccgaac
     2761 ctaaaatcgc cggaactgac tgtgacagta cttcgaactt tctaccttta gttaccaaaa
     2821 ggatgcttag tctttaccca cgcctgaggc atctgcgcgt gcgccgcacc tggcgtggac
     2881 tttatcctgc cacgccggac ggttctccga tcgtcggatt cactcatcaa ttctgcaatt
     2941 tatttttggc gattggaatg tgcggacagg gttttatgat cggtcccggt cttggtgcaa
     3001 ttatagccga aatactaacg gacaatactc ataaatatga tgagattctc gaccagtgga
     3061 atctctatcg taattttact ggccaggaaa ttttaaaata aaataagagg agcttctatg
     3121 actattccta tttcaaaacg agcccaagcc ttaaaaccgt caccaactat gtctgtaacc
     3181 gctttggctt caaaaatggc ggctgaaggc atttccataa tcaactttgg ggtcggcgaa
     3241 ccagatttta atactccgga aaatatcaaa caagccgctc atcaggcgct ggatgccaac
     3301 ttcactcatt ataccgtctc agccggcatt ctggaattac gccaggcaat ttgtgataaa
     3361 ctcaaacgtg ataataatct ggactattca cccgacgaaa tcatcgtagc acccggcgga
     3421 aaagccgcca taatcaccac cctcgccgca atttgtgacc ccgacgatga aattatcatt
     3481 cccgccccct attgggttag ttatccttcc caagtcgaga tgttggatgg caagccggtt
     3541 ttcatcgcca ccgacgaatc aaccaatttc aaaattaccg ccgaccagct ggaatcggtt
     3601 attaagaacc tttcgcaacc gaaagcacta atccttaatt caccgaacaa tccaaccggc
     3661 gccgtttatt cgccaaccga attagccgct atcggcgcag tttgtctaaa acacaatttg
     3721 gcagtgattt ccgacgagat ttacgaaaaa ttagtttacg gctctgctcg gcattactca
     3781 atcgtccagg ttttgcctga aatccgatcc attacaattg tcataaacgg cttcagtaaa
     3841 gcctatgcga tgactggctg gcgcgtaggt tatgccgccg gaccgcgtga ggtcatcaag
     3901 cgggcaaccg ccattcaaga gcataccgcc tcctgcgtta cttcaatcac ccaaaaagcc
     3961 gcagttgccg ctttgaatgg ggatgacagc agtatcgagg ctatgcgcca ggaatttgaa
     4021 agacgccgtg attatttagt aaatgaattg aaacagattc cattagttga ctgcccgtta
     4081 ccagatggcg cattttacgc gttcccgaat atttctcact atataaaaaa caacaagatc
     4141 ggcatccgtg attcggttca attatgtgag tatttactga aaaatttcca catcgctttg
     4201 gtccccggtt cggcttttgg ttgcgatgac tgcctgcgct tttcctatgc caccagtatg
     4261 gaaaatatcg tcgaaggggt caaacgtttc aaatctggat tggcaagttt atagnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctgga gtataggcaa caaagatata
     4441 gacgccactg ctgactaatt ttccttgttc attccgccca tcccagtaag cttgttgtcc
     4501 aactacttcc ccatccttag gatccaggtg ccggataaac gtgccgtcta gaagcattat
     4561 tttcacttcc gaatcttgta acaatccgtc aatagtcaaa ggttggtcgg caggaatccg
     4621 aaaaggcgat gggaaagtgt tcggcttctg gtattgattg ccataatagg cataaggcga
     4681 tttatacacg gaaatacctt tgtcggtaga aatatacact aagccccgcg gtggatcgaa
     4741 agctatatcg agaatattat cgcttaacaa cccagaattc ttactggtaa atccctcttt
     4801 atcgtttagc cagataccat tataagtata taattttact ccggaattta ccgtcgtaat
     4861 ccacttatta tttaagccat caaccttgat gcggcattct ttggcaaagg caatattggt
     4921 caaagccgcg taatcaatgg cgtcgaagac tctagctggg aaactttccg aaacgctcgc
     4981 tgattgaata ccgcccgctg tcattatcca caatttattt tccagatcga atgccagaga
     5041 ataaaccata ttgctggcga gaccgtcgct ggttgttacc ctgtaccatt gatcatcact
     5101 tttatcaaat aaagtatttt tgtaatcaag cacggcaatt ccgccgttgg aaccaggtgg
     5161 ttccgaagtg gcttcagagg caaaccacac tctgcccgcc gcgtcaaacg cgattgaagt
     5221 aactaaataa ttcaaataat tccctgactc ttgcccgcta aaatgtaccc aacggtcatc
     5281 gggcgtcaag accgcaatga aactatcgtt ttgagcatat tgattggcaa tccataaatt
     5341 gttctgatta tcaagcgcca tataagctat tcccaaataa tcatctcgcc caccataacc
     5401 cgctgaacca gccagtttac cgcctgtagt atcgtaaacg acataattct cgagatcatc
     5461 taaattaaat ctcaacaaac cgccgccgc
//
LOCUS       DAOMJU010000110         5368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_16412_length_5368_cov_18.312818, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000110 DAOMJU010000000
VERSION     DAOMJU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16412_length_5368_cov_18.312818"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLO58_08740"
     CDS             complement(<1..633)
                     /locus_tag="PLO58_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQO74915.1"
                     /translation="MIKAVNIKSSAENRRVLIADKNPDLCHTLSTFLVKNGCNCQSTN
                     DSATTLRLLEESEFDFLIIDLDLARYDGNSILDKAHIISPRTFIILTTSNASVETAVG
                     AFRKGAIDYLIKPLNQRVLLDQINQAIQLREILNENRFLRREIHNQKNPDQIIGESPS
                     IKNIYHLISKVAHSSSNILITGRSGTGKELVARAIHQNSPRANAPFIAINC"
     gene            970..1752
                     /locus_tag="PLO58_08745"
     CDS             970..1752
                     /locus_tag="PLO58_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HQO74916.1"
                     /translation="MSKEDQEIIKLREEVMSQEVLPRHIAIIMDGNGRWAKQRGLPRV
                     AGHNEGINSVREVTRECGSLGIKVLTLYTFSVENWSRPKSEVSALMTLLLHTIRNEID
                     ELHKNNVRLTLIGHLTDLPDDPLKGLMEGVEKTSQNTGLNLNLALSYGGRVEILDATR
                     AIAKDVLEGRISLDDIDEKLFSDYLYTRGMPDPDLLIRTSGELRLSNFLLWQMAYTEI
                     YVTETLWPDFRNKELLLAIREYQKRERRFGKTSEQITSPSES"
     gene            1755..4124
                     /gene="bamA"
                     /locus_tag="PLO58_08750"
     CDS             1755..4124
                     /gene="bamA"
                     /locus_tag="PLO58_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HQO74917.1"
                     /translation="MIKSKPISLFLLILMLFSLIMAQEEAGIMILGLAVQGHKTISES
                     SIKIQSGLVEGRRVTQEDIAQAIQRLWKLKMFSDIQILLDKETSEGVFLVIKVEEYPR
                     LEKFEVKGNKKIKKSKIEEELNLVQGRVLSANLIAEVKRKVKALYTKEGYLLAQVDAE
                     ITDGTKENSKNLILKIQEGKKVRIDEINFVGNEHFSDSRLRRVMKDTHERNLFILRTG
                     EFDETKYIEDKEKLRTFYRKEGFRDFKIISDSISYTPNKERMLITINLYEGPRYKYRN
                     INFSGNTVFTTEQLQMLLGIKPGDYYNEELLQKALYDRLNGAYMDRGYLFCQIMPLEV
                     PVAENEIDLTIEITENSIVSIDQININGNTKTNENVIRRELRMFPGDIFSRDALMRSQ
                     NEIYMLNYFANVTPDVVPVSENAVDVELTVEEKSTDQANLSFSISQVYGLVGGGGFQF
                     NNFRGRGQQLAISYQQGTSYSVYSTSVTPYKALSLSFADPWIFDSPNLVGASVSYSER
                     GSGSSYYYPYNLTIYGGSLRWGRRLRWPDNYFRISWSYGAYKKSYTDIGSAYLEEVLE
                     GKDRTTSIGLTQVISRDSRDRPEFPTTGSVFSWVTNLTGGLLGGTEHFTKNTFTLDYY
                     LPTIWKLILYNHVEFGVINKLKKGTAVPPDERFIMGGSGMIYGTALRGYDDNEVGPTY
                     YYGGYYYPYGGETMLKYSIEYRVPIANNPTIYALAFAEAGNVWKYMRDTDPFDLKRSV
                     GVGIRFYMPALGLLGIDFGYGFDDIDPEGTTGYGKPEGWKTHFIFGTAY"
     gene            4158..4679
                     /locus_tag="PLO58_08755"
     CDS             4158..4679
                     /locus_tag="PLO58_08755"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQO74918.1"
                     /translation="MKKVVFIILGLLMIGASDLAQAQIKVGYVNSDRILSEFDEAREA
                     QTKLDIEAKKLEDQYRGMLTKLDSLQKDYDRQKMMMSETRRKTKETEISQLQESIQRF
                     QVEKLGPDGEIYKKQAELVTPILEKIKTVIAKVGKDGKYDFIFDTVAGNILYAEPAHD
                     VTDKVIYELKRTQ"
     gene            4682..>5368
                     /locus_tag="PLO58_08760"
     CDS             4682..>5368
                     /locus_tag="PLO58_08760"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQO74919.1"
                     /translation="MQLTLGQIARLVNGAVKGDENILINDVAEIQHARSGTITFLANP
                     KYAKYLASTAATAVIVDENFNGNFPNLILVKNPNLAFSIVLAHFRPELPLPAPMIHPT
                     AVIAKDARIGDDVYIGPFVVIESGAQISDKVTVFGHCYVGQYVNIGSGTVIYPNVSIY
                     HKCIIGQKVRIHAGVVIGSDGFGFVRVENGIEKIPQAGRVVIHDEVEIGANSTIDRGT
                     IGDTVIGRGTK"
BASE COUNT         1595 a          970 c         1285 g         1518 t
ORIGIN      
        1 gcaattaatg gcgataaagg gtgcgtttgc gcggggactg ttttggtgaa tagcccgcgc
       61 tactagttcc tttccggtac cgctccggcc agtaatcaga atgttagatg aagaatgggc
      121 gaccttggaa attagatgat aaatgttctt aattgaggga ctttcgccga taatttggtc
      181 gggatttttt tgattgtgaa tttctctgcg aaggaaccgg ttttcattta gaatttctct
      241 aagttgaata gcttggttaa tttggtcgag caggacgcgc tgattgagtg gttttatcaa
      301 ataatcaata gcgcctttac ggaaagcgcc aacggcagtt tcaacggaag cattcgaggt
      361 ggttagaatt atgaaagtgc ggggactgat gatatgagct ttgtccagaa ttgagtttcc
      421 atcgtatctc gctaaatcca gatcaataat taaaaagtcg aactccgatt cttccagcag
      481 gcgcagagtg gtagcgctgt cgtttgtcga ctggcagttg cacccgtttt taaccagaaa
      541 ggtacttaaa gtgtgacata agtccggatt tttatcggct atcagtactc gtcggttttc
      601 tgcacttgac tttatattga ccgccttgat catttaaatt tcttagattc aatatagaac
      661 agagtaagtt ttaatcaaaa acatttttta aatctcggaa atgagaagta attaacggct
      721 cgttttaagt aaatgaatat tttacgagga acaatcctaa ttgcctgccg taaaatgagc
      781 cgaagttatg actattaaga aaacacaaac ctaaaacatt tctcttaatc gagaatctgg
      841 aaattgaaca gttcatttag accagccgat ctattcttta ttacctaatt caccaaaatt
      901 ttaaatggtt ttcattgact ttataggctt tatataataa tttctgtagt ttatttaact
      961 ttgaatcaaa tgtccaaaga agatcaggaa ataatcaaat tacgagaaga ggtaatgagc
     1021 caggaggtct taccgcggca cattgctatc attatggatg ggaacggtcg ctgggcgaaa
     1081 cagcggggac taccgcgggt agccggtcac aacgaaggaa tcaattcggt tcgggaagtt
     1141 acgcgcgaat gcggtagtct tggcatcaaa gtgctgactc tatatacctt ctcggtggag
     1201 aactggtcac gccccaaatc ggaagtttct gcccttatga ccttgctgtt gcacacaatc
     1261 cgtaatgaaa tagatgagtt gcataaaaat aacgtgcgtc tgactttaat tgggcacctt
     1321 acggatttgc cggacgatcc gttgaaaggg ttgatggaag gagtcgaaaa gaccagtcaa
     1381 aataccggac tgaacctgaa tctggcattg agttacggcg ggcgggttga aatattagat
     1441 gccactcgtg ccattgctaa agatgtgctg gaagggcgaa tcagccttga tgatattgat
     1501 gagaagctat tttccgatta tttatataca cgcggtatgc ccgatcctga cttattgatc
     1561 agaacgagtg gagaactgcg cctcagtaat tttttactct ggcaaatggc ttataccgag
     1621 atatatgtta cggagacttt atggcctgat ttccgtaata aagaactact tttggcgatc
     1681 cgtgagtatc agaagcgaga acgtcgtttt ggaaaaacca gtgaacaaat taccagtcct
     1741 tcagaaagtt gagtatgata aaatctaagc cgattagttt gtttctattg atattaatgc
     1801 tgttctcgct tataatggct caggaagaag ccgggataat gatcctcggg ctggcggtgc
     1861 aaggtcataa gactatttcg gaatccagca tcaaaattca gtccggatta gttgaaggcc
     1921 gaagagttac tcaagaagat attgctcagg ctattcaacg actttggaag ctcaaaatgt
     1981 tttccgatat tcaaattttg ctcgataagg aaacgagtga aggagtgttt ctggtaataa
     2041 aagtggaaga atatccgcgt ttggagaagt tcgaggtcaa gggcaataag aaaatcaaga
     2101 agagtaagat agaagaagaa cttaacctag ttcagggaag ggtcttatct gctaacttga
     2161 tcgcagaagt caagcgcaaa gtcaaggctc tttatacgaa ggaaggttat ctcctggctc
     2221 aagttgatgc ggaaatcacc gatggcacta aggagaacag caaaaacttg attctcaaga
     2281 ttcaggaagg gaaaaaagtt cggattgatg aaatcaactt tgtcggcaat gagcattttt
     2341 cggatagtcg tttacgtcgg gtaatgaaag atactcatga acgcaatcta tttattttgc
     2401 gcactgggga attcgatgag actaaatata tcgaggataa agaaaaattg cgcacctttt
     2461 atcgcaaaga aggtttccgg gattttaaaa tcatttcaga ttcgatttct tatactccga
     2521 ataaggagcg tatgttgata acaatcaatc tttatgaagg accacgttat aaatatcgca
     2581 atataaattt tagtggaaat acggtattca ccactgagca gctccaaatg ttactcggca
     2641 ttaaaccggg tgattattat aatgaggaat tgttgcagaa agctctttat gatcgcctca
     2701 atggagcata tatggatcgc ggttatttat tttgtcaaat aatgccgctg gaggttccgg
     2761 ttgcggagaa tgaaattgac cttaccattg agataaccga aaattcgatc gtatcaattg
     2821 accaaattaa cattaatggc aatactaaaa ccaacgaaaa tgttattcgg cgcgaattga
     2881 gaatgtttcc cggcgatatt tttagtcgcg acgccttaat gcgcagtcaa aatgaaattt
     2941 atatgcttaa ttatttcgcg aatgtgactc cggatgtagt accggttagt gaaaacgcag
     3001 tagatgttga gctgactgtg gaggagaaat ccaccgatca ggcaaattta tctttcagta
     3061 taagccaggt ttatggtctg gtgggtggag gcggatttca attcaacaat tttcgaggac
     3121 gcggtcaaca gttggcaatc agttatcagc aaggaacaag ctactccgtc tatagtacgt
     3181 cggttacgcc gtataaagct ctctccctta gtttcgctga cccctggatt ttcgattcac
     3241 ccaatttagt cggtgctagt gtttcctatt cggaaagagg aagcggcagt agttactatt
     3301 atccgtataa tttgacgatt tacggaggat ctctgcggtg ggggagacgt ttacgctggc
     3361 ctgataatta ttttcggatc agctggtcgt acggggctta taaaaagagc tacactgaca
     3421 ttggcagtgc gtacttagaa gaagtgctgg aaggtaaaga tcgaacgacg agtatcggac
     3481 ttactcaggt aataagtcgt gacagtcgtg atcgcccgga atttccaacg actggttctg
     3541 ttttcagctg ggtaacaaac ttgaccggtg gtttgttagg tgggactgag catttcacca
     3601 aaaatacttt taccttggat tactacttac cgacaatttg gaaactgata ctgtataatc
     3661 atgttgaatt tggagttatc aataaattaa agaaaggtac cgctgtgcca cccgacgagc
     3721 gatttataat gggcggcagt ggtatgattt acggtacagc cctacgcggt tatgacgata
     3781 atgaagtcgg cccgacttat tattacggtg gttattatta tccgtatggt ggcgaaacaa
     3841 tgctgaaata ctctattgaa tatcgggtgc cgatcgcgaa caatccgact atttatgcct
     3901 tggcatttgc ggaagctggc aatgtctgga aatatatgcg agataccgat ccttttgacc
     3961 ttaaacggtc ggtaggtgtt gggatacgct tttatatgcc ggcgctcgga ttattaggta
     4021 ttgattttgg ttatggattt gacgatattg accctgaagg tacgaccggt tatggtaaac
     4081 ctgaagggtg gaaaacccat tttatttttg gtacagctta ttaacaataa aaacaaatgt
     4141 gtaacaatgg aggaaaagtg aaaaaagtag tgttcattat tcttggattg ttgatgatcg
     4201 gagccagtga cttagcgcag gctcaaatta aagtcggcta cgtcaattcc gatcggattc
     4261 tgagtgaatt tgacgaagcg cgggaagcgc agactaaact ggacattgaa gccaagaaac
     4321 tggaggacca gtatcgcggt atgcttacta aattggacag tctgcagaaa gactacgatc
     4381 gtcagaaaat gatgatgagt gaaacgcgac gcaagactaa ggaaaccgaa attagtcaat
     4441 tacaggaatc gattcaaaga ttccaggtcg aaaagctcgg accggatggc gagatttaca
     4501 agaaacaagc cgaattggta actccgattc tggaaaaaat caaaaccgtc atagccaagg
     4561 tggggaaaga cgggaagtac gattttattt ttgatacggt tgctggcaat atcttgtatg
     4621 ccgaaccggc tcacgacgtt actgacaaag tcatctatga gttaaaacgc actcaatagg
     4681 tatgcaatta accttagggc agattgcccg gttagtaaac ggcgcagtta aaggcgacga
     4741 aaatattctt atcaatgacg ttgccgaaat tcagcacgct cggagtggta cgattacttt
     4801 tttagccaac ccgaaatatg cgaaatattt agcaagcacc gccgcaacgg ccgtgatagt
     4861 agatgagaat ttcaatggca attttcctaa tttgatattg gtaaagaatc ctaacctcgc
     4921 tttttcgatt gttctggctc attttcgacc ggagttgcct ttgccagctc ctatgattca
     4981 ccccacagcc gtgattgcaa aagatgccag aattggagat gatgtatata ttgggccatt
     5041 cgttgtcata gaatccggtg cgcaaattag tgacaaggtc acggtctttg gacattgtta
     5101 tgttggtcaa tatgtaaata tcggtagcgg aacggttatc tatccgaatg tcagtatcta
     5161 tcataaatgt ataattggtc aaaaggtgcg cattcatgcc ggagttgtga tcggaagtga
     5221 tggatttggt tttgtacgcg ttgaaaatgg cattgagaaa attccgcaag ccggtagagt
     5281 cgtaatccac gacgaggtag agatcggcgc taattcaacc atagatcgag ggacaatcgg
     5341 tgatacagtc attggccgcg gtaccaaa
//
LOCUS       DAOMJU010000111         5325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_16584_length_5325_cov_20.039848, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000111 DAOMJU010000000
VERSION     DAOMJU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16584_length_5325_cov_20.039848"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLO58_08765"
     CDS             <1..249
                     /locus_tag="PLO58_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74920.1"
                     /translation="VLIRITDPEFQTPSAEDLALGGGLALFLGFPLLILLNAPMNFWH
                     NSLGGYWLTLALMLVYLVILWVVWRLVGYIHFKLPENK"
     gene            438..1355
                     /locus_tag="PLO58_08770"
     CDS             438..1355
                     /locus_tag="PLO58_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HQO74921.1"
                     /translation="MLNRYKILDKRIVLNGGEDASILVFINPDEAEKRMLVDQFMIDE
                     HTLNSALDPDELSRLEYEPNHIAFIFKRPRSITAKENILFKVTSTGVFLFKDQIIIVA
                     SEDIPIFEGKIFNRVNSNQDILLKLILRAICHFLEHLKAINMVTDEFQEKINTSLENK
                     YLIDLFSLAKSLVYYLNAINSNGVLLEKLKINTVKIGFSQEECELLDDIIIENTQCVK
                     QAEIQSNVLASLMDARASIVSNNLNVLMKTLTIITIGIMVPTLIVSIFSMNVDLPLPR
                     NHPSSFWIIIGMALVSVVAFFFFWKRKKW"
     gene            1527..4022
                     /locus_tag="PLO58_08775"
     CDS             1527..4022
                     /locus_tag="PLO58_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQO74922.1"
                     /translation="MILRNVKVLSITLSVLVILISSANIMAQPPAGAPRPTSGRLSGT
                     VLIKNTDKPIEYAAITLHGLQDSSIITGTITNEDGIFILDKIPFGRYFAKISFIGYKT
                     AVINNIAITPPKPEVVLGTVYLEPVAIDLSSVTVTGEKKALAYNLDKKVITIDNSVGT
                     IGGTATDIMQTIPSVTVDIDGNVSLRGSSSVTLLVDSRPSQIISLDEIPASMVERVEV
                     ITNPSARYDPEGMSGIINIVLKKKRSPGYNGMLLVNAGTGGKYNGSINLNYRYNKFNL
                     FANYDMRMGHRLGTGETIRESIRNDSTFYLNQYQNSENRNRFHNIKLGGDYFINDYNT
                     ISLSMLYNLRSFSNQNNTEYLNTDYNAVGYNQFTRLSDGDTDNQGQEYGLFYKKTFPQ
                     PQREWTIDLFYSTFSRNSDQSITQQAVLTDSLITVPPDLENIFSDDANRVITARTDYV
                     HPIGEHRRIEAGYRGTFRHNDSDYRRFNYNYTIADWFPDSLASNHYIYDANIHAVYGI
                     YSRELGRLQSQLGLRIEQFNTNNNLVTIDSSFQKNYFSMFPTIHFRYALTENQGLQVS
                     YSRRVHRPHTWQVSPFVDYSDPYNLSSGNPKLKPEYIDVAEIEHSYENRKTSINTSLF
                     YRQVNGMITRLTELQEGGITFTTYQNLNNGVSYGIESVISHNLTPWWRINTNISYFNN
                     RLYGTGISIADANESQSWNARVNSFFRLGENTEMQIMLFYFSPTVGVSGGGRGFHGPG
                     QGRMQENYFVNLGAKHNFLDGKMSAFVRVSDIFKTQKHGQTIYGDNFKTTSLRHPESQ
                     VIFLGISYKINEGIKERKKRTDEENEYPDMEEM"
     gene            4669..>5325
                     /locus_tag="PLO58_08780"
     CDS             4669..>5325
                     /locus_tag="PLO58_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HQO74923.1"
                     /translation="MSRTIIVKIIQVQFPNNPMNNFDIPKLRGFFANRYPKCDLIHNH
                     LSDGRFKYGYPSIQFKVLNRIPTLIGLSEGFDILKEVFQDVDELVIAGEKAKIWEKSI
                     QIREDDFGQTDSFFDYQFTSPWMALKEENYETFKTLDRIEKQQFLRHLLRENLKTVSK
                     GFCYNIPNIESVKVEGYFKPRPMNFKNIRMVCFTGEFTTNFLLPDYLGIGKQVARGFG
                     T"
BASE COUNT         1604 a         1063 c         1032 g         1626 t
ORIGIN      
        1 gtgctgattc gtatcaccga tccggaattc caaacgccgt ccgccgagga cttggctctc
       61 ggtggcgggc tcgctctatt tctgggcttt ccgctattga ttttactcaa tgctcctatg
      121 aatttctggc ataactccct cgggggatat tggttgactc tggcgcttat gctggtttat
      181 ctggttattc tctgggtggt atggcgcttg gtaggatata ttcattttaa attaccggag
      241 aataaataaa gcgcggctac ggttcgggag agagtacagc gacagctaat tttgctcgtc
      301 aatttgccct cgaacgcaga gattgaattt taataaaaaa tttttctttg ttagccaata
      361 attgcttaag ttcgccgtgc tttctaaaaa gaatatttca taagaatcaa cgagcagatt
      421 tcagacaaga ggtaaaaatg ctcaatcgtt acaaaatctt agacaagcga attgtactga
      481 atgggggcga ggatgcttcc attctggttt ttattaatcc cgatgaagcg gaaaaaagaa
      541 tgttggttga tcaatttatg atagatgagc atactctaaa ctccgctctc gaccccgatg
      601 agctctcgcg tctggagtac gaacccaacc acatcgcttt tattttcaag cgaccgcgca
      661 gtatcaccgc taaggaaaat attcttttca aagttacttc gaccggcgta tttcttttta
      721 aagatcaaat tatcatcgtg gcatcggaag atataccgat attcgaaggg aaaattttta
      781 atcgggtcaa ctcgaatcag gacattttac tcaagctgat tttacgggcg atttgccatt
      841 ttctggagca tttaaaagcc atcaacatgg tcacggatga attccaggag aaaataaaca
      901 cttcattaga aaataaatac ctaatagact tgtttagtct ggcaaagagt ttagtctatt
      961 atcttaatgc catcaattcc aatggcgttc tccttgaaaa actaaaaatc aataccgtca
     1021 aaattggatt cagtcaggaa gaatgcgaat tgcttgatga tattataatc gaaaatactc
     1081 aatgtgttaa acaagccgaa atccaatcga atgttttagc cagcttaatg gatgcccgcg
     1141 cttcaatcgt cagcaacaat ctcaacgtct tgatgaaaac cttgactatt attacgatcg
     1201 gtattatggt gccgacgctg atcgtcagca ttttttccat gaacgttgat ctacccttac
     1261 caagaaatca tccttcttct ttttggataa ttataggaat ggctttggtt tcggttgtag
     1321 ctttcttttt cttctggaaa cggaaaaaat ggtaagcctg acgtattctt gttaaaaacc
     1381 tcttgaatcc agttaacttt tctaaaaatg ctacccagtt gtaccgcaag cttctattcc
     1441 ggctaataat tctaaagtat gtttaacctt tttacttttt cggaatcaaa tagccgaaca
     1501 attttaacca aaggatttta cgatttatga ttttgcgaaa tgttaaggta ctatctatta
     1561 ctctttcagt actggtaatt ttaattagta gtgccaatat aatggctcaa ccaccagccg
     1621 gtgctcctcg accgacatcc ggcagacttt ccgggacagt tttaattaaa aatactgata
     1681 agccgattga atatgcggca atcactttac acggtctcca ggattccagc attataaccg
     1741 gtacgattac caacgaggat ggaatattta tcctcgataa aattccgttt ggtcgttact
     1801 ttgccaagat tagttttatc ggatacaaaa cggcggtcat taataatatt gcgattacac
     1861 cgccgaaacc ggaggtggtt ttgggaacgg tctatctcga gccggtggca attgatttat
     1921 cgtccgttac tgtcactgga gagaaaaaag ccctcgcgta taatctcgat aaaaaggtca
     1981 tcactattga taatagcgtt ggaaccatcg gaggcacagc caccgatatt atgcagacta
     2041 ttccgtcggt caccgttgat attgatggaa atgtcagtct acggggttca tccagtgtta
     2101 ccttgctggt tgatagccgc ccgtcacaga ttatcagcct cgatgaaatt ccagctagta
     2161 tggttgaacg agtagaggtt atcaccaatc cttcagcacg ctatgatccg gagggtatgt
     2221 ccggtattat taatattgtt ctcaagaaaa aacgctcgcc cggctataat ggtatgttac
     2281 tggtaaatgc cggaactggc ggcaaatata acggttccat taaccttaat taccgctata
     2341 acaaattcaa tttatttgct aattatgaca tgcggatggg tcataggctt ggaactggcg
     2401 aaactatccg tgagtcaatc cgcaatgatt ctacttttta tctaaaccaa tatcagaatt
     2461 ccgaaaatcg taaccgtttt cacaacatta aactgggcgg tgattatttc attaatgatt
     2521 ataatactat ttcactatca atgctctaca atctgcgctc tttctctaat caaaataaca
     2581 ctgagtatct caacactgat tataatgctg tgggatacaa tcagttcact cgcctctctg
     2641 atggtgacac ggataatcaa ggtcaggaat acggtttatt ttataaaaaa acttttcctc
     2701 aacctcagag ggaatggaca attgacttat tttattcaac attctcacgg aattctgacc
     2761 aatctattac ccagcaagcg gttttaacgg atagtcttat aactgttcca ccagatttgg
     2821 aaaacatttt ttcagacgat gctaatcggg ttataactgc ccgcactgat tatgtgcatc
     2881 ccataggcga acatagacgg atcgaagcag gctaccgcgg tacttttcgg cataacgatt
     2941 cggattaccg acgctttaat tataattata cgatagcgga ttggtttccg gatagtctgg
     3001 cttccaacca ttatatttat gatgcaaata ttcacgccgt ttatgggatt tattcgcggg
     3061 aattaggccg tttacaatct cagttaggtc taagaatcga gcagtttaat accaataata
     3121 acttagtgac gattgactcg tctttccaga aaaactactt cagtatgttt ccaactattc
     3181 attttcgcta cgctctgacg gaaaatcagg gtttgcaggt cagttacagc cgtcgggttc
     3241 accgacctca tacctggcag gtttctcctt ttgtagatta ttccgatcct tataatctct
     3301 cttccggtaa ccccaaactc aaaccggaat acattgacgt cgccgagatt gagcattctt
     3361 acgaaaatcg aaaaacttcg atcaacacct cgctatttta tcgtcaggta aatggaatga
     3421 taactcgcct cacggaatta caagaaggcg gtatcacttt tactacttac caaaatctaa
     3481 ataacggtgt ctcttatggc atcgagagcg tcatcagtca taatttaaca ccctggtggc
     3541 ggatcaatac caacattagc tattttaaca accgtcttta tggaaccggg atttccatcg
     3601 ccgatgccaa tgagagtcag agctggaatg cccgggtcaa ttccttcttc cgtcttggcg
     3661 aaaataccga aatgcaaatt atgcttttct atttttctcc gacagtcggc gtttcgggag
     3721 gcgggcgtgg ctttcatgga cccggtcagg gacgtatgca ggaaaattat ttcgtcaacc
     3781 ttggcgccaa acacaatttc ctcgatggga aaatgtcggc tttcgtaaga gttagcgaca
     3841 ttttcaagac tcagaaacat ggccaaacga tttacggtga taatttcaaa actacatcac
     3901 tgcggcaccc ggaaagtcag gtgattttct taggtatttc ttataaaatc aacgagggca
     3961 tcaaagagcg caaaaagaga accgacgaag agaacgaata ccccgatatg gaagagatgt
     4021 agctgagtac tatctgtcag tctgttaact gtgatagatt ttgccgcctg tcaaaataaa
     4081 tagttaaaaa ctattaattc cattttctat cactaactaa caatctcata ctgaatctaa
     4141 ttttcagtat tctaaaaccg attacattta aattattttt atcagaaaat gggtgctaat
     4201 taaaagcact cagagattgt aatttagcgt ctggaataga tcagtcagcg ctataataat
     4261 cacctttttt gggttattat aacaaaacca atccttataa agcagataat attgaatctt
     4321 aatccgaaat tagagatagg gaaattattt agccattttt aagatgcgag aatcagtaca
     4381 aatatagaat ttgtttatga tacgaaaagt agcttgatat tctaaaatta atttataaat
     4441 tattttggtt tggaagtttg gtatgaatag agaaatattt gatctgctaa aatttgaaat
     4501 tggttttgta agtcggcaat attagaagtt taggcaaagt ttttaacgac ctattcccga
     4561 agacttttaa tattcatgct ttcccagatt tttgatcggt aaaattgagg tccagatggt
     4621 taacggctat tatgaaatca actaaatgaa gaaagtgtat caagacgaat gagtaggaca
     4681 atcatagtaa aaattatcca ggttcagttc cccaataatc cgatgaataa tttcgatatt
     4741 cctaaactgc gcggcttttt cgctaaccgt tacccaaaat gcgatctaat ccataatcat
     4801 ctatctgacg gtcgttttaa atatggctat ccctcaatcc aattcaaagt gctgaaccgt
     4861 attccaactc taatcggttt atccgaagga tttgacattc taaaggaagt gtttcaagat
     4921 gtagatgaac ttgtgattgc gggtgaaaaa gccaaaatct gggaaaagag tatccaaata
     4981 cgagaagatg attttggcca gactgattct ttttttgatt atcaattcac ctctccctgg
     5041 atggcgctta aagaagaaaa ttatgagacc tttaaaactc ttgaccgaat tgaaaaacaa
     5101 cagtttctgc ggcacttatt acgtgaaaat ttaaaaaccg tttcgaaagg tttctgttac
     5161 aatataccta atatcgaaag tgttaaagtc gagggatatt tcaaaccgcg tccgatgaac
     5221 tttaaaaata tccgcatggt ctgcttcacc ggagaattta cgacaaactt cctcttgcct
     5281 gactatctcg gtatcggtaa gcaagtcgct cggggatttg gcacg
//
LOCUS       DAOMJU010000112         4865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_18840_length_4865_cov_17.227443, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000112 DAOMJU010000000
VERSION     DAOMJU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18840_length_4865_cov_17.227443"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLO58_08785"
     CDS             <1..768
                     /locus_tag="PLO58_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQO74924.1"
                     /translation="GKEGFNFDIEIRMGSGAYVCGEETALIESLEGYRGEPRNRPPFP
                     VDTGFQNHPTIVNNVETFLDAALICVKGAKWFNEHGTEKSSGTKLFSVSGDCEKPGIY
                     ELPFGITIEQLLKEVKGENAKAVQIGGASGRCVARKDFNRTIAFEDISSGGSIIVLGP
                     QVDMLKVAINFMEFFVEESCGQCTPCREGNVKLLEGLKLLEEGRCSASYLNELMRLAE
                     TIQLASKCGLGQSSPNAFVSIIENFKSEILGRVPEKA"
     gene            780..2711
                     /locus_tag="PLO58_08790"
     CDS             780..2711
                     /locus_tag="PLO58_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HQO74925.1"
                     /translation="MEDNEKVMTESTVRAPKSTTPSTIKAPEDISAIGATIKVTIDGK
                     EIRVPMGTTILNAARMLGINIPTLCHHNDLCISGVCRICVVEVEGMRTLQASCAYPIS
                     QPIVVHTHTRKVRQARRHVVDLLLARHYGECYSCVRNNNCELQQLAHEYGVDFYRFGH
                     VDKPTYGRDNSSYAIVRDMDKCILCRRCVRTCIDLQEVGVYEAFERGNQTRIGTFMDN
                     PMAELICINCGQCINRCPTGALHANDPSDAIWAAIDDPTKHVVIQTAPAPRSAIGECF
                     GLPAGTPLTFEMNTAMRYCGFDKVFDTNFSADLTIIEEGTELILRLYKALVKKEPVSL
                     PLFTSCSPGWVKYIEHFYPEMLSHLSSAKSPQQMFGAVIKTYYAELNNIDPADIVTVA
                     LMPCTAKKFECNRPEMNSSGFKDIDYGLTTRELAGMFQEAGIDLPNMPKSDFDEPFGT
                     RTGSGVIFGATGGVMESAIRTVYELITGDRIDNLFERARIMPVRGFEGIRYAELPIAK
                     VGPVPDLLKHLIPNWDWLRGATLKVAVAHGTANAKKVLDNIKSGGKLSECHFIEFMAC
                     PGGCLGGGGMPIPTNEEIRAARAQAIYAEDEASEVRKSYENPAVVRLYNEFLVDGPCG
                     HKSHKLLHTYYTPRGKKLV"
     gene            2839..4029
                     /locus_tag="PLO58_08795"
     CDS             2839..4029
                     /locus_tag="PLO58_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQO74926.1"
                     /translation="MPNRFIYLDHNATTPLHPEVKNTITDMFDVFGNPSSLHTVGRIA
                     RQKIEQAREIVANFIGAAPDEIIFVGSGSEANNSVLNSLSCPSQVCRLERNLRDEIIT
                     TSIEHPCVLETSKCLRERGTTVHYLPVDITGKVKLDVLKELLSEKTALVSVMYANNEI
                     GTIQDIKAIAEIAHAQGAFFHTDAVQAVGKIPVNVQDLDVDFLAFSAHKIYGPKGVGA
                     LYVKRGTPFCPLIRGGHQERGRRAGTENTIGIVAFAKAIEMRKQEMSDEAVRLRRFKK
                     MLIDGIKYKIPDVQFNGHPTDTLPGTLNISFAGAEGESILLYLDLEGIGVSTGSACAS
                     GSLEPSHVILATGVGAELAHGSIRISMGRENTADDIYYTVSALERVVKKVRDMSTTYQ
                     SKLV"
     gene            4026..4646
                     /locus_tag="PLO58_08800"
     CDS             4026..4646
                     /locus_tag="PLO58_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HQO74927.1"
                     /translation="MSNQSGWLYSEIVKDHFMNPRNILRDDENFVADGIGITGNIKCG
                     DEMQFLIQVDKQKGVITDCRWRTYGCASAIASTSVLSEMVKGMKLEDAYRITPKDVVK
                     NLGSLPEHKIHCSVLGDKALRAAIDDYYRRNGMNEKIKHEEARVICQCMNVTDHEIEE
                     AVLEGARTYLELQERTKLGTVCGQCQEEATELLKKYIAKHFGDNRK"
BASE COUNT         1312 a         1098 c         1118 g         1337 t
ORIGIN      
        1 ggcaaggaag gtttcaattt cgacatcgag attagaatgg gttccggcgc ttatgtctgt
       61 ggcgaagaaa ccgccctgat cgaatcgctg gaaggctacc gcggtgaacc gcgtaatcgc
      121 ccgccttttc cggttgatac cgggtttcaa aatcatccaa ctattgtcaa taacgtcgag
      181 acttttctgg atgcggctct catttgtgtc aaaggcgcta aatggttcaa tgaacacggc
      241 actgaaaaat ccagcggcac caaacttttc agtgtttccg gcgactgcga aaaacccggc
      301 atttacgaat taccatttgg tattaccatc gaacaattgt taaaagaagt caaaggcgaa
      361 aatgcaaagg cggtgcaaat tggcggcgct tccggtcgtt gtgtagcccg taaggatttc
      421 aaccggacta ttgcctttga ggatatttcg tccggcggtt cgattattgt tttgggaccg
      481 caggtggata tgctcaaggt cgccatcaat tttatggaat ttttcgtaga agaatcctgt
      541 ggccaatgta ctccctgccg tgaaggcaat gtcaaactgc tcgagggttt aaaactgctc
      601 gaagagggac gctgttcagc ttcctattta aatgaactga tgcgtctggc ggaaacaatt
      661 caactagctt ccaaatgcgg tttgggtcaa tccagtccga atgcctttgt ctcaattatt
      721 gaaaatttta aatcagaaat tttaggccgt gtgcctgaaa aagcttaaag aggatattta
      781 tggaagataa cgaaaaagta atgactgagt ccacggttcg cgcacccaaa agtactacgc
      841 catccaccat taaagctccc gaagatatca gtgcgattgg tgcgactatc aaagtcacaa
      901 ttgatggaaa agagattcgc gttccgatgg ggaccacgat tctgaatgcc gctcgcatgc
      961 tcggcatcaa tattccaact ttatgtcatc ataacgactt gtgtatcagc ggtgtttgcc
     1021 gtatttgcgt agtagaggtc gaaggtatgc gcactctcca ggcttcctgt gcctatccaa
     1081 tcagccagcc gattgtagtt catactcaca cccgcaaagt tcgccaggcg cgacgtcatg
     1141 ttgttgacct tttgcttgcg cgtcattatg gcgaatgtta ttcctgcgtc cgcaacaata
     1201 actgcgaatt acaacaatta gcccatgaat atggtgttga tttctatcgt tttggccatg
     1261 ttgataaacc gacttatggc cgcgataact ccagctatgc aattgtgcgt gatatggata
     1321 aatgtattct ctgccgccgt tgcgtgcgta cctgcattga tttacaggaa gttggagtct
     1381 atgaagcctt tgaacgtggt aaccaaaccc gcattggcac ctttatggac aatcctatgg
     1441 cggaactgat ttgcatcaat tgcggacaat gtatcaaccg ttgtccgacc ggcgctttgc
     1501 atgccaacga cccaagtgat gctatctggg ctgctattga tgatccgact aaacatgtcg
     1561 tgattcagac cgctccggcg ccgcgttccg ccattggcga gtgtttcggt ttacctgctg
     1621 gcacccccct gacttttgaa atgaataccg ctatgcgtta ctgcggtttc gataaagttt
     1681 tcgatactaa cttttccgcc gatttaacca ttattgaaga agggaccgaa ctaattttgc
     1741 ggctttataa ggctttagta aagaaagaac ccgtgtctct gccgctgttt acttcctgtt
     1801 cgcctggatg ggtaaaatat atcgaacatt tttacccgga aatgttatcg cacctgtcat
     1861 cagccaagag tccccaacag atgttcggcg ccgtcatcaa aacctattat gctgagctta
     1921 ataacatcga tccggccgat attgttaccg ttgcgctgat gccctgtact gccaagaaat
     1981 tcgaatgcaa tcgtcctgag atgaattcat ccggttttaa agatattgat tacggcttga
     2041 cgacgcgcga attggccggg atgttccagg aagccggaat tgatttacct aatatgccta
     2101 aatcagattt cgacgaaccg ttcggaactc gcaccggctc gggcgtcatt ttcggtgcaa
     2161 ccggtggcgt tatggaatca gctattcgga ctgtctatga attaatcacc ggtgatcgca
     2221 ttgataacct attcgaacgg gctcgcataa tgcccgtgcg cggtttcgaa ggcatccgtt
     2281 acgctgagct tcccattgct aaagtcggtc cggtgccgga cttactaaag catttgattc
     2341 ctaattggga ttggctgaga ggtgcaactc tgaaagtagc ggtagcacat ggaactgcta
     2401 acgccaagaa ggtgcttgat aatattaaat ccggcggcaa attaagtgaa tgccacttca
     2461 tcgaatttat ggcttgcccg ggtggttgtc tgggtggtgg tggaatgccg attccgacca
     2521 atgaagaaat tcgagccgct cgagcgcagg ctatctatgc tgaggatgaa gccagcgagg
     2581 ttcgcaaatc ctatgaaaat ccagccgtgg tcaggttata caatgaattt ctcgttgatg
     2641 gaccctgtgg ccataaaagt cataaactct tgcatactta ctatacgcca cgtggtaaaa
     2701 aattagtgta acaatcatcc tcctctttat ctctaagaag ggcaatcttt taatagtttg
     2761 ccctttttta ataaatgagg tcgtagtaat tttgcaattg agtaattgaa aaaaagaatt
     2821 catttcatag aggataaaat gccgaatagg tttatttatt tagatcacaa tgctaccact
     2881 ccattacacc cggaagttaa aaatactatt accgatatgt tcgatgtatt tggcaacccc
     2941 tccagtctcc acaccgtcgg tcggatcgct cgccaaaaaa tcgaacaagc ccgtgaaatt
     3001 gtcgcaaatt tcatcggcgc cgcgcccgat gaaattatct tcgtcggaag cggtagtgaa
     3061 gccaacaatt ctgtccttaa tagtctctcc tgcccttcgc aggtttgtcg ccttgaacgc
     3121 aatctgcgcg atgaaatcat cacaacctct atcgaacacc cttgcgttct ggaaacttcc
     3181 aaatgtttac gagaacgcgg tacgactgtt cattatttgc cagtggatat taccggaaaa
     3241 gttaaactcg atgtacttaa ggaattactg tcagaaaaaa ccgcattggt ttcggttatg
     3301 tacgccaata atgaaatcgg tactattcaa gatataaaag ctattgcgga aatagcccat
     3361 gctcaaggcg cttttttcca taccgatgcc gttcaagccg tcggtaaaat tcccgttaac
     3421 gttcaagacc tcgacgttga tttcttagca ttttctgccc acaaaatcta tggcccgaaa
     3481 ggggtcgggg cactttatgt caaacgtggg actcccttct gtcccttaat tcgcggcggt
     3541 catcaagaac gcggccgccg tgctggaact gaaaacacga tcggcattgt ggcttttgcc
     3601 aaagccattg agatgcgtaa acaagaaatg tctgatgaag ccgtacgtct gcgccgtttt
     3661 aaaaaaatgt taattgacgg gattaagtat aaaattccag atgtgcaatt taatggtcat
     3721 cccaccgata ctttgcccgg aacactgaat atctcctttg ccggggcgga aggcgaatca
     3781 attttacttt acttagacct cgaaggaatc ggggtttcaa ccggctctgc ctgcgcctcc
     3841 ggttcgcttg agccttcgca cgtaatcctc gccacaggcg ttggcgcgga actggcgcac
     3901 ggctcgatcc gcattagtat ggggcgtgag aatactgctg acgatattta ttatactgtt
     3961 tcagctctgg agcgcgtggt aaaaaaggtg cgtgatatgt caacaactta tcagagtaaa
     4021 ttggtatgag caatcaatct ggctggctat actccgaaat agttaaagac cattttatga
     4081 atcctcgtaa tattctaagg gatgacgaga actttgtcgc cgatgggatc ggtattaccg
     4141 gcaatatcaa atgcggtgat gagatgcagt ttctcatcca ggttgacaag caaaaaggtg
     4201 ttataacaga ttgccgctgg cgaacttacg gttgcgccag tgcgattgcc agcacttccg
     4261 ttctttcgga aatggtcaaa ggtatgaaac tcgaagacgc ctatcgcata actcccaaag
     4321 atgttgtgaa aaatctcggt agtttgccgg aacacaaaat ccactgctcg gtactgggcg
     4381 acaaagcctt gcgcgccgct attgacgatt attatcggcg taacggtatg aacgaaaaaa
     4441 tcaagcacga agaagcccgc gttatctgtc aatgtatgaa cgttaccgat catgaaatcg
     4501 aagaggcggt cctcgagggt gcccgcactt atttagaatt acaagaacgc actaaactcg
     4561 gcacggtctg cggtcaatgt caggaagaag ccaccgaatt acttaaaaaa tatatcgcaa
     4621 aacacttcgg cgataaccgg aaataatcct gaatatcata ttttaagtac ttttaagtgc
     4681 tttaaagttc tataagaaaa tttccgccat aaggttgttt ttaagcatta acgcatgaca
     4741 gactgattat atggatgttt agaattacat tttgtcgcaa agataattta atttattata
     4801 agcacttaag cgcgcttaaa tgaacttaat tgcttagcgc actctgcggt tggctatttt
     4861 cttaa
//
LOCUS       DAOMJU010000113         4765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_19411_length_4765_cov_15.903822, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000113 DAOMJU010000000
VERSION     DAOMJU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19411_length_4765_cov_15.903822"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2051
                     /locus_tag="PLO58_08805"
     CDS             <1..>2051
                     /locus_tag="PLO58_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HQO74928.1"
                     /translation="GNRALKVVVTEAGSNQWDLQIVADSIPVVPGGNYRFTVWAKAQQ
                     AGAQVNFTVGNYSYSEYTAIRPANLTTTWTAYTKDFTITDNETVIRAPIHFSYSANVG
                     NAIYIDNFRIVYLDEIKKPVVVEAESGVVGSDFSILEEDSITYVTIQTNLAAYNPGSS
                     ARVISYEVTFPDTGSYDLFVRLRTGADTFNDDSFFYGKGFGEKDPSVDSVWVCINGLA
                     DAGFSGPNEIVRDAGGLGSGVWKWVNLSRNVYQGSVESLVTFIVDDPDSLTQTFQIGA
                     RENGLDIDKIAFGKSYLYYTVGNLDNREPGSTDLPGEVWEGPPLAHKQPKFVGNIYSS
                     PQISNFEAYWNQVTPENAGKWGSVEGTRDVMNWSGLDAAYKLAKDNGFPFHFHVLVWG
                     AQQPSWINDLTPEEQLEEIREWFEAVAERYPDIDFLEVVNEPLPGHNPPDGTSGRANY
                     KEALGGNGETGWDWVIKAFEMAREIFPEGTRLMINDFNIISSSSSTSQYLKLIRLLQE
                     RNLIDIIGEQGHAFTTTAAVATMKKNLDSLASTGLPIQITELDIDGPSDAVQLQSYQR
                     IFPTLYEHPGVEGITLWGWRRGLWRDEQGAYLINQDGSERPALVWLRTYLDTLNLNVM
                     VKEDIINVLDEFSLGQNYPNPFNPTTTINYQLPTTSHLTIKVYNLLGKEVATLVDDVK
                     QA"
     assembly_gap    2052..2151
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2152..3180)
                     /locus_tag="PLO58_08810"
     CDS             complement(2152..3180)
                     /locus_tag="PLO58_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQO74929.1"
                     /translation="MTRITIEEVAKQANVSKGTVSAVINGKNSVNPATREHILKVMKD
                     LNFRPRGMARNLKNGDQVRCIGIIIKDLNYPFYTSIAAGAREYANSKGYSVIVASSEN
                     DHECEKKFTHLFSIKDIKGAIIAPIVEGEAEIDHLFKLKRINYPFVLLEDVKGIQANV
                     VAIDNLSAIKTAVKYLIDNGHTKIVHFAGPPQSSHTQERIEGFRHAFSESPLIFNKNL
                     IISIGSRHEEAYGNTMNYFKNRSREDYPTAIVCFNDQQALAVMTALKELNIRIPEDIS
                     IIGNDDIIYAKIYPVPLTTIRAPQREIGYKAAEILIRNIESSTALPNEKIVLGTDLVI
                     RESSRVLN"
     gene            3780..>4765
                     /locus_tag="PLO58_08815"
     CDS             3780..>4765
                     /locus_tag="PLO58_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HQO74930.1"
                     /translation="MDQISRNVIRFNCCKSFTSLITHFAKQVTLILFSLLILCPNILE
                     AVSSNMDSYVSVEAIDGGFPLSVAGHTAPLYASSQDYPGVLRVLKLFQSDLKMVTGIE
                     PVIAIDTIPKSKEIVIVGTIGKSPIIDALIQSNQLDVSDISGKWETFLIQRIEKPYPD
                     VEQALVIAGSDKRGTIFGVFDLSEKIGVSPWYWWADVPVKKKDCIYIKPGRHTLGEPK
                     VKYRGFFINDEAPALSNWAYEKFGGFNHEFYVNVFELLLRLKANHLWPGMWGKYFGSD
                     PENPRLADEYGIVMGSSHCEPLLYNNDPGAGLWNSQTMGPWRYDINRENIYKV"
BASE COUNT         1370 a          886 c         1015 g         1394 t
ORIGIN      
        1 gggaatcggg ctctaaaagt cgttgttact gaagctggat caaatcagtg ggatttacaa
       61 atagtggctg atagtattcc ggtagttcca ggcggaaatt atcgctttac cgtctgggct
      121 aaggctcaac aagccggggc gcaagttaat tttaccgtcg gtaattattc ctatagtgaa
      181 tatacggcta tccgcccggc aaatcttacc actacctgga cagcctatac aaaagatttt
      241 acgataacgg ataatgaaac cgtcataaga gcgccgattc atttcagcta ttcggcgaat
      301 gtgggcaacg cgatttatat tgataatttt cggatagtat atcttgatga gatcaaaaag
      361 ccagtagttg tcgaagccga atctggagtt gtgggaagtg atttcagtat attagaggaa
      421 gattcgatta cttatgtgac tattcaaacg aatttagcag cttacaatcc tggcagtagt
      481 gcccgggtaa tatcctacga agtaactttc ccagataccg ggagctatga cctttttgtc
      541 cgtttacgaa cgggagcaga tacatttaac gatgacagct tcttctatgg caaaggattt
      601 ggagaaaaag atccctcagt tgattctgtt tgggtttgta taaatggact tgcggatgcc
      661 ggattctcag gaccgaatga aattgttcgt gacgcaggcg gtcttggttc tggggtttgg
      721 aaatgggtta atctttcacg aaatgtatat cagggttccg ttgagagcct tgttactttt
      781 attgttgacg atccagatag tttaactcaa acttttcaaa ttggcgcacg cgaaaatgga
      841 ttggatattg acaagattgc ctttggtaag tcatatctat attatacggt aggaaatctt
      901 gataatagag aacccggttc aacagacctg ccaggtgaag tatgggaagg tccaccgctg
      961 gctcataaac aaccaaaatt tgttggaaat atttacagtt ctccgcagat aagtaatttc
     1021 gaagcttact ggaaccaggt tactccagaa aatgccggca aatggggtag tgtggagggc
     1081 actcgtgacg ttatgaattg gagcggatta gatgctgcct ataaacttgc caaggataat
     1141 ggtttcccgt ttcattttca cgtgctcgtt tggggtgctc agcaacctag ctggataaac
     1201 gatttaacac ctgaagagca acttgaagaa attagagaat ggttcgaagc cgttgccgaa
     1261 agatatcccg atattgattt tctggaggtg gtcaatgaac cattgcctgg tcataatccg
     1321 ccggatggaa ctagtggacg agctaactat aaggaggctt tgggcggtaa cggtgaaacc
     1381 ggttgggatt gggtgattaa ggcatttgaa atggcacgtg agattttccc cgaagggact
     1441 agactgatga taaatgattt taatataatc agtagctcaa gcagtacatc ccaatatctt
     1501 aagctcatta gattgctcca agagagaaat ttaatcgata taattggcga acaagggcat
     1561 gcatttacta caactgccgc tgtagccaca atgaaaaaaa acctcgattc tcttgcttct
     1621 accggattac caattcaaat tactgaatta gatattgatg gaccttctga tgcggtccaa
     1681 ttacaaagtt atcaaagaat tttcccgact ctttatgagc accctggagt agaaggaatt
     1741 accttgtggg gctggcgacg gggtctttgg agagacgaac agggagctta cctgattaat
     1801 caggatggtt cggaaagacc ggctttggta tggttaagaa cctatctgga tactttaaac
     1861 ctcaacgtta tggtcaaaga agacattatt aacgttctcg atgaatttag cttaggtcaa
     1921 aattatccca atcccttcaa tccgacgacc accatcaatt atcaattacc tactactagt
     1981 cacttgacta tcaaagtgta taacctgctc ggaaaagaag ttgccacttt ggttgatgat
     2041 gtcaaacaag cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttagtttag
     2161 cacccgggat gattcgcgga taaccagatc ggttcccaga acaattttct cattggggag
     2221 agcggttgag gactcgatat tgcgaatcag gatttcggcg gctttataac cgatttcgcg
     2281 ctgaggcgcc cgaatcgtgg tcagaggtac cggatagatt ttagcgtaga taatatcatc
     2341 attgccgatt atggaaatat cttccggaat tcggatattc aattccttaa gagcagtcat
     2401 aaccgccaga gcttgctgat cattgaaaca aactatagcg gttggataat cttcccggct
     2461 tcggttctta aaataattca tagtattacc ataggcttct tcatgacgcg aacctatgga
     2521 tatgatcaaa tttttattga aaataagcgg actttcacta aaagcgtgac gaaagccctc
     2581 aatccgttcc tgagtatgcg aggactgggg cggtcccgca aaatggacaa tcttggtgtg
     2641 cccattatca attaaatatt tgactgccgt tttaatggcg gataggttgt caatggcaac
     2701 cacattagcc tgaatacctt tgacgtcttc aagtagaaca aagggatagt tgattctttt
     2761 taatttgaaa agatggtcaa tttccgcctc accttcgacg attggcgcta ttattgcgcc
     2821 tttaatgtct ttgatagaaa acaagtgcgt aaattttttc tcgcattcat ggtcattttc
     2881 cgaactggcg acaatcaccg aatacccttt actattagca tattctctgg cgcccgccgc
     2941 aatcgaagtg taaaatgggt aattcaagtc tttaatgatt ataccgatac agcgaacttg
     3001 atcaccgttt ttcagattcc gagccatacc tcgggggcga aagttaaggt ctttcattac
     3061 cttaagaata tgttctcgcg tagcgggatt gacagaattc ttaccattta ttacagcaga
     3121 taccgttcct ttagaaacgt tggcctgctt agcaacttct tcaattgtaa ttctcgtcat
     3181 agaaaactcc ggttcataat catacgatcc ataagtgagt ccaaattttt tggtttaaga
     3241 aaaaactcat tgccgccaat actgacactt cgtttatagt tgtaaagaaa aaatagccgt
     3301 aataccatac ctgaccgatt ccaatcacca aaaaaatcaa ctgctaattt aagtaaaatt
     3361 tttatagaat ggtattaatt atagtaaccc attttatcat attttcattc atttgagatt
     3421 ttgataaaat tctatcaagt atctttaagt tctattaaga aatttttagt cctaaaattg
     3481 gtttttaaac atcaatcaat gcgcggttgc atgaaaatac ttaagaggaa ttaaaaaaac
     3541 tctgtgtttt ccatatcctc ttcgtgcccc cgtgtcgcaa tttaaggaca ttgaatcagg
     3601 caagttgaat tttccaatga aatcttgctg tttctgccgg atatagttca ttatacaaaa
     3661 ttaattttta aaaatgcact atctatttga ttaaacgcct agatattatt aaaattaaaa
     3721 cggttcaata attgtaatat tggttgtttt gataaaggaa aaaataaatg gaggacaaaa
     3781 tggatcaaat ttcgcgaaat gtgattcgtt ttaattgttg caaatcgttc acttcattga
     3841 taactcattt cgcaaagcaa gtaacgttaa ttctcttctc cttgttgata ttatgtccta
     3901 atattttaga agccgtcagt agcaatatgg attcctatgt ctcggtggaa gcgattgacg
     3961 gtggttttcc gctttcagtg gctgggcata ccgcgccact atatgccagc tcgcaggatt
     4021 atccgggagt attgcgagtt ctgaagctat tccaatcaga cctaaaaatg gtaactggca
     4081 tcgagccggt gattgccatt gatacgattc ccaagtctaa ggaaatcgtc atcgtgggca
     4141 ccataggtaa aagtcccata attgatgctt tgattcaaag taatcaattg gatgtcagtg
     4201 atatctccgg caagtgggaa acttttctaa ttcagagaat agaaaagccg tatccggatg
     4261 ttgagcaagc gttggttata gccggtagcg ataagcgcgg cacaattttt ggcgtgttcg
     4321 atctttccga gaaaatcggg gtctcgccct ggtattggtg ggctgatgtt ccagtaaaga
     4381 aaaaggactg tatttatatt aaaccaggac gacatactct cggtgagccc aaagtaaaat
     4441 atcggggatt ttttatcaat gacgaagcgc cggctctaag caattgggct tacgaaaaat
     4501 tcggcggttt caatcacgaa ttctatgtta atgtttttga acttctgcta aggctaaaag
     4561 ctaatcatct ctggccaggt atgtggggca aatactttgg ctccgacccg gagaatccaa
     4621 gattagccga tgaatatggt atcgtaatgg gctcgtcgca ctgtgaacct ctgctttata
     4681 ataatgatcc cggtgccggt ttatggaatt cacaaactat gggaccctgg cgttatgata
     4741 ttaatcgtga aaatatttat aaagt
//
LOCUS       DAOMJU010000114         4667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_19956_length_4667_cov_18.577190, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000114 DAOMJU010000000
VERSION     DAOMJU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19956_length_4667_cov_18.577190"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1672
                     /locus_tag="PLO58_08820"
     CDS             233..1672
                     /locus_tag="PLO58_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74931.1"
                     /translation="MSSSRNKLTWAVFLLTVGWLIPLWAQTRLTGGKRPSWIDHPAPG
                     YYIGISRRWAEEADSRADALNNAKRQIIESLGGVIESEFIDRIIETDGTVESVDAFTD
                     SRIKVVAKNIINVKPEKVYTEQWQEGKGRQKKILYQTYMAVYFDENAHRAFMKQLVEE
                     TVQLGEIQLSSAITLARQGRIFLAIDQIKAATQHLQPLTEITGIAPTDLIKIKTLNGQ
                     MLDWINLLQNGIRIEGLGDKQTTKWGAGLPEPLQVSVFWQESGKRYPISGLPVEFKLL
                     AGKAAFNPYSQTDASGCAFCSVREIRTAGRVEFEAQVTFPEGYQMAQTSTRFNLLPDN
                     KVIVMVSETNFYHPVNTPILGNLLLQKLTESGFHILEANPFSRFTGSQIEQMSPTEIQ
                     QAATNSGADLILLASVASDQPNRVQDGFYFAHARGVLKVYNISQQAFVGNYLVEDKNA
                     GNSPENAGAKAIKKVSDALIQKFSDEFGL"
     gene            1846..>4667
                     /locus_tag="PLO58_08825"
     CDS             1846..>4667
                     /locus_tag="PLO58_08825"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQO74932.1"
                     /translation="MLISLNKKIILIGTILAFLPLSVLATNYYVRKSGNDSNNGLTPA
                     TAWKTVTKATNYNLQPGDTVFIGTGIYNEQLIPSRSGSSTAYIVYYGDYKGLKTQDPA
                     GRVTIGGLAAGSPVFVPQSASQNNFTFTLANIINNGDGTSTIKVGIRNNNRSNLNNTA
                     ISLPSGVTALYPANGSTWTSPKNIKYTINNPYSNPFYCIRYNRSSGNVYRGGSDTLIY
                     KLTTQQAEALTSVRIRATTVSGTTGDVTLSVVYQSPSGTSYDIACKVNDKDNLVFQGI
                     TFGYVSNKAIDLYNTTNIVIRDCDTRNATNHGIYASSWDLHGVLTIESDSILSGNSAG
                     ISVQASNAHTDFVININNNYISDCGKGIELNRAYLKNGNINNNIIRNSSSHAIYIYQP
                     TEKALSVSHNQISNVTSGSGLYLYQCGVQNLDDNKISNTGSHGMYISDATSQYVPKIN
                     TICNNEVSNVNNNCHGIYLDRVYVGQIANNLIFNITSHGIYADQVSQFAILALDSNLV
                     YNCTGSGIYTMQTMSFNSVKGNTIYLVQNGLYLYANNFFSVANFSDNRIFSYSLQGIY
                     CKYLQNTTIENNLVYGCTGSGSSTYGIQIYNDNLYSNTIKNNTIYNGGYYGIYGNRTT
                     GSWRNNIVTGSTYGFRDNVSSGITATYNCAYNNTYNYSNITPGTGSITQNPLFVDPDG
                     ADNILGGNNWLDDDLHIKSTGGSYHFGAWSFDDQDSPCLDTGDPNDDYSREPEDNGDR
                     INIGCYGNTPQASKKKSACPMTAVYKNFPNNKWVMIGVPIAPTEGTPPGDPLAIFGDD
                     FGGKMPDGTNWTCIHWVTEDSVMEYYEYGDGTIYQPPTPYPGLGYFVWQNTGAPVNVD
                     VRGCPVDHCLLEVAQAPYVDWESEYGVALGFNQFANPYYFTIDWSNTEIFKYASADKT
                     GTPVKYTLSEAAANGWISQYAYTWDH"
BASE COUNT         1344 a         1012 c         1009 g         1302 t
ORIGIN      
        1 aagtaacaat accatcgcca gctggatagg tttgcatttt ttcattagaa tttcctcttg
       61 taattgtttt taatcacgtt tggtatttat ttcactcatt attctgcgcc attaatataa
      121 tactttagcg gcaaagttaa aactgccagt ttccgtgcca gataagttcg gtttgaaatt
      181 ataaaacttt attggtagat ttaattaaca gagctgaaaa aaggaaagcg agatgtcgag
      241 tagcagaaac aaattaacct gggcagtctt tctactaacg gtaggttggc taattccgct
      301 ttgggctcaa acgcgattaa ccgggggaaa gcgtcccagc tggattgacc acccggcgcc
      361 tgggtattac atcggcattt cgcgtcgttg ggcagaagaa gctgacagcc gcgccgatgc
      421 tttgaacaac gctaaacgtc aaataatcga atccctcggc ggcgtcattg aaagtgaatt
      481 tattgatcgc atcatcgaaa ctgacggtac agtggaatca gtggacgctt ttaccgattc
      541 acgcatcaaa gtcgtagcca aaaatattat aaacgttaag ccggagaagg tctatacgga
      601 acagtggcag gagggcaaag gtcgtcaaaa gaaaattcta tatcagacct acatggcggt
      661 ttacttcgac gaaaatgctc accgcgcttt tatgaagcaa ttagtggagg aaaccgttca
      721 gctgggcgaa atccaactga gttcagcaat caccttagcc cggcaaggtc gtattttcct
      781 ggcaattgat cagataaagg cggctactca acatttacaa ccgctgaccg agattaccgg
      841 gatagcgccg actgatctga taaaaataaa aaccttaaac ggacaaatgt tggactggat
      901 taatctcttg caaaacggga tccgcattga aggtctgggt gataaacaaa ctaccaaatg
      961 gggcgccgga ttacctgaac cgttgcaggt gagcgtattt tggcaggaaa gtggcaagcg
     1021 ctacccgatt tccggactgc cagtcgagtt taaattgctg gcgggcaaag ccgcatttaa
     1081 tccctacagt caaaccgatg ccagcggctg tgccttttgt tcggtcagag aaatccgtac
     1141 cgctggcaga gtcgaattcg aggctcaggt tacttttccc gaaggctatc agatggcaca
     1201 aaccagtacc cgctttaacc ttttaccgga caacaaggta atcgttatgg tctctgaaac
     1261 caatttttac cacccggtta atactccgat tctgggtaat ctgcttctcc agaaattgac
     1321 cgaatccggc tttcatattc ttgaggctaa tccttttagt cggttcacag gctcgcagat
     1381 tgaacaaatg agcccaaccg aaattcagca agccgccacc aatagcggtg ctgatttgat
     1441 tctgctggcg agcgttgctt cggaccaacc taatcgggtt caggacggat tttacttcgc
     1501 gcacgcccgc ggcgttttga aagtttataa catttcccaa caggcgtttg tcggcaatta
     1561 tctggttgag gataaaaacg ccgggaattc accagaaaac gccggagcca aagctatcaa
     1621 aaaggttagc gatgctctta tacaaaaatt cagcgatgag tttgggcttt gattgaaatt
     1681 tgttttgtgg tataattcag gtggcttttt attgaaatgg ttagaaagaa ctcataatgg
     1741 actgtagagt aggtatttta agcatttgat ttttatgaca atggtcacaa acactacttc
     1801 aataatttat gttcttaaaa agattttaat tggcggagga aattcatgtt gatttccctt
     1861 aataaaaaaa ttatccttat tggaacgata ctggcatttc taccgctttc ggttttggct
     1921 acgaactatt atgtccggaa aagcggcaat gacagcaata acggtttgac tccggcaacc
     1981 gcctggaaaa cagtcactaa agccactaat tataatttgc aacccggcga tacggtcttc
     2041 atcggaacgg gaatttacaa cgagcagtta ataccctcgc gttccggctc gtcaactgct
     2101 tatattgttt attatggtga ttacaaagga ttaaaaactc aggatccggc tgggcgggta
     2161 accatcggag gtcttgccgc cggtagcccg gtattcgtgc cccagtctgc ttctcagaat
     2221 aatttcacct tcaccctggc aaacatcatc aataacggcg atggaaccag cacaatcaag
     2281 gtcggaatcc gcaataacaa tagatccaat ctaaacaata cagcgatcag tttaccttca
     2341 ggagtcaccg cgctataccc agccaacggc tcgacctgga ccagtcccaa aaatatcaag
     2401 tacaccatta ataatccata cagtaatcca ttctactgca ttagatacaa tcgttcgagt
     2461 ggaaatgttt atagaggtgg ctctgataca ttaatctata aacttactac tcagcaagcc
     2521 gaagcgctga cctcagttag gattagagct acaacggtta gcgggacgac tggagacgtt
     2581 actcttagtg tagtttacca gtctccatcg ggaacttcct atgatattgc ctgtaaggta
     2641 aacgacaaag ataacttggt atttcagggt attaccttcg gttatgtcag caataaagcc
     2701 atagacctct ataacaccac taatatagta atcagggact gtgatacgag aaatgccacc
     2761 aatcatggta tttatgcctc cagctgggat ctgcatggag tcctgactat tgaatctgat
     2821 tctattctaa gtggtaacag cgctggaatc tcagtacaag caagcaatgc gcataccgac
     2881 tttgtcatta atatcaataa taactacatc tccgattgcg gtaagggcat tgaactgaac
     2941 cgtgcgtatc tcaaaaatgg taatatcaat aataatatca ttcgcaatag ttccagccat
     3001 gctatttata tttaccaacc cacagaaaag gcgctttcgg tttcgcacaa tcagattagt
     3061 aatgtaacca gcggtagtgg actttatctc tatcaatgcg gcgtccaaaa tctcgatgat
     3121 aacaaaatta gcaataccgg ttcgcacggg atgtatattt ccgatgccac ttcccagtat
     3181 gtccctaaaa taaataccat ctgtaataat gaggttagca atgtaaacaa caattgtcac
     3241 gggatttacc ttgatcgggt atatgttggg cagatagcta ataatctcat atttaacata
     3301 accagccatg gcatttatgc tgatcaggta agccagtttg cgattttggc acttgattcc
     3361 aacttggttt acaattgcac gggttcaggt atttatacca tgcaaacgat gtcatttaat
     3421 tcggtcaagg gcaatactat ctatctggtt cagaatgggc tttatcttta tgccaataat
     3481 ttttttagtg tggctaattt cagtgataat cgcatttttt cttattcgtt acagggtatc
     3541 tattgtaaat accttcagaa cactactata gaaaataatc ttgtttatgg gtgcaccggt
     3601 agcggttcaa gtacttacgg tattcagata tacaacgaca atttatattc taataccatt
     3661 aaaaataaca ctatttataa cggtggctat tatgggattt acggtaatcg cacaaccggc
     3721 agttggcgca acaatatcgt taccggctca acttatggct ttagagataa cgttagttca
     3781 ggtattaccg ccacttataa ttgcgcttat aataacactt acaattattc caatatcaca
     3841 ccgggaactg gttcgattac acagaaccct ttgtttgttg atccggatgg agccgataat
     3901 attttaggcg gaaacaattg gctggatgat gacctgcata tcaaaagcac gggcggttct
     3961 tatcatttcg gggcatggtc atttgacgat caggattcac cctgtctgga tactggtgat
     4021 cctaacgatg attatagtcg agagccggag gataatggcg accgcattaa tatcggttgt
     4081 tatggcaata ctccccaagc gtccaagaaa aaaagtgcct gcccgatgac ggcggtttat
     4141 aaaaactttc ctaacaataa atgggttatg attggagttc cgattgcgcc tacagaaggt
     4201 actccacctg gcgaccccct cgccattttc ggcgatgatt tcggtggcaa aatgccggat
     4261 ggcaccaatt ggacttgcat tcactgggta acggaagact ccgttatgga gtattatgaa
     4321 tatggtgatg gcacgattta tcaaccgcct actccttatc ccgggctcgg ctatttcgtc
     4381 tggcagaata ccggcgctcc ggttaatgtg gacgtcagag gttgcccggt tgatcattgt
     4441 ctcttagaag ttgctcaggc gccttatgtt gattgggaat ctgaatatgg agttgcgctc
     4501 ggttttaatc aatttgctaa cccctattat ttcacgattg attggtccaa taccgagatt
     4561 ttcaaatatg cctccgctga taaaaccggc accccggtaa aatacactct ctctgaagcc
     4621 gcagctaacg gctggatcag tcagtacgcc tatacctggg atcacaa
//
LOCUS       DAOMJU010000115         4547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_20700_length_4547_cov_18.804987, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000115 DAOMJU010000000
VERSION     DAOMJU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20700_length_4547_cov_18.804987"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLO58_08830"
     CDS             complement(<1..322)
                     /locus_tag="PLO58_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HQO74933.1"
                     /translation="MNYKSVMPIWIAFLLLVSVFIANLNSANAPDIHTWEMQEIVLQA
                     EGEYENFYTDVTCWIELTGPEFAKRIYGFWDGANIFKVRFVATKPGKWYLRSGSNQPN
                     DNGLN"
     gene            complement(398..3565)
                     /locus_tag="PLO58_08835"
     CDS             complement(398..3565)
                     /locus_tag="PLO58_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQO74934.1"
                     /translation="MKQILEKYLANLSDTFNRGDAREESYYKHLDDLIKQCAEIQNIK
                     NIEVTILPKKTDAGNPDFRVWDGQNHITGYIEAKDPSITDLDYIEDSEQLKRYISTFP
                     NVILTNFYEFRLYRDGERIRRVMIGRPVIAQKLKTKPPLENIEQFIELFDLFFSFSLP
                     KVQNARSLAVELAKRTRFLRDEVIAVEMAENGSKGQKQIAGFYEAFKTYLIPTLTAEQ
                     FADLYAQTITYGLFAARTRVTSERDEFNRKIAFDYIPHTIGILRDIFHFISLGDPPKS
                     LQIIVDDIVEILNVADVNKILHEYQRAGKGRDPIIHFYETFLATYDPKIRERRGVYYT
                     PEPVVGYIVRSIHSILKSHFNLADGLASEEVKLLDPAGGTLTFPAEAIRLAADEFKSK
                     YGEGGLHLWIKKHILANFHAFELMMAPYAIGHLKIGFILDELGYKMAEDERFKLYLTN
                     TLEMEEIKEITIPLLSSLSEESHFAEKVKKEQPILVILGNPPYSGISANINKWTERLL
                     KEDLDGAQSYYKVDGQPLGERNPKWLQDDYVKFLRFAQWKIQQSGYGIVGMITNHSYL
                     DNPTFRGMRQSLMKTFDEIYILDLHGNSLKKETTPAGGKDENVFDIRQGVAIALFVKN
                     KKPKESRVFHADLYGLREEKYQWLQKNDFQKNNYEKIKPTSPYYFFKKIDTGKVKQYL
                     KWKQITDIFPVNSVGIVTARDHFVISFDKRDLKNRILQLQNLAHPDEIILQAFDLKEK
                     FGWKIKDSRQEIANISDLDIYIKEILYRPFDKRYLFYHQSLIERMRFEVMQHMLEENI
                     GLVTIRRSRNPEKWNFAFVANKLIAGATAITSLDINYLFPLYLYPEQSKKDNPGLNLT
                     TMLIFEPGEKYGRKPNIAPIIFSRLAEHYPKRPSPEEIFYYVYAILYSNIYRETYVEF
                     LKIDFPRVPFTADYEVFKNLGKSGKQLADLHLLKSPALDPPIARYQGSSPNDRIEKVI
                     YQKDEQRIYINNDKYFEGIAPEVWNYYIGGYQVLHKYLKDRKGRTMDDAPHYCRIVTA
                     LSKTIEIQKQIDAIYPDVEKEIIKF"
     gene            complement(4322..4394)
                     /locus_tag="PLO58_08840"
     tRNA            complement(4322..4394)
                     /locus_tag="PLO58_08840"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4359..4361),aa:Lys,seq:ctt)
BASE COUNT         1279 a          894 c          884 g         1490 t
ORIGIN      
        1 tgtttagtcc attgtcattc ggctggtttg aaccactgcg caagtaccat tttcccggct
       61 tagtagcgac gaaacgcact ttgaaaatat tagcgccatc ccagaaaccg taaatccgct
      121 tggcaaattc cggcccggtt agttcgatcc agcaagttac atcggtatag aaattttcat
      181 attctccttc agcctgcaag acaatttctt gcatttccca tgtgtgaata tccggcgcat
      241 tagctgaatt cagattggca atgaaaacac ttactaaaag cagaaaggca atccaaatcg
      301 gcatcacact tttgtaattc atctaaactt agtatccttt cataacgaaa tgaaaaattt
      361 gaataatcgc acgactaatt tcgataaaat tcattgttta aaactttatg atttcctttt
      421 ctacgtcggg atagattgcg tctatttgct tctggatttc aatggtttta ctcaaggcgg
      481 ttactatgcg acagtaatgg ggcgcatcgt ccatagttct tcccttacgg tcttttaaat
      541 atttatgtaa tacctgataa ccgccgatgt aataattcca gacttccggt gcaatgcctt
      601 caaaatattt gtcattatta atgtaaatgc gctgttcatc tttctggtaa ataaccttct
      661 cgattcggtc gtttgggcta ctgccctggt atcgggcaat cggcggatcg agcgccgggc
      721 ttttaagcag atgcaggtca gccagttgtt ttcccgattt gcctagattt ttaaacactt
      781 cataatcagc ggtaaaaggc acgcgcggga agtcaatttt caagaactcg acataggttt
      841 cgcggtaaat attgctgtat aagattgcgt aaacataata gaaaatctct tccggcgacg
      901 gtcttttggg ataatgctcc gccaatcgtg aaaaaattat gggcgcaata ttaggttttc
      961 ggccgtattt ttcgcccggc tcaaaaatca gcatcgtagt cagattcaga ccgggattgt
     1021 cttttttcga ttgctccgga taaagatata aaggaaatag gtaatttatg tccaaagatg
     1081 taatggcagt cgcaccagca atcaatttat tggcaacaaa agcaaaattc catttttccg
     1141 gatttcttga ccttcgaata gttaccaacc caatattctc ttccagcatg tgttgcataa
     1201 cttcaaagcg catacgttct atcagagatt gatggtaaaa taggtagcgt ttatcaaatg
     1261 gacgatacaa tatttctttg atataaatgt caagatcact gatgtttgct atttcttgcc
     1321 gggaatcttt gatcttccaa ccgaatttct ctttcaaatc gaaagcctgt aaaatgattt
     1381 cgtccggatg agcaagattt tgtaattgta atattctgtt ttttaaatcc cttttatcaa
     1441 aactaatgac aaaatgatcg cgcgccgtaa caatacctac actattcacc ggaaaaatat
     1501 cagtaatttg tttccatttc aaatactgct ttacttttcc tgtatctatc tttttaaaga
     1561 aataataagg gcttgtcggt ttaattttct cataattgtt cttttgaaaa tcgttttttt
     1621 gcagccattg atacttttct tcacgcagtc catataaatc tgcgtgaaat accctagatt
     1681 ccttcggctt tttatttttc acaaataacg caattgccac gccctgacga atatcaaaca
     1741 cattttcgtc tttgccaccg gcgggagttg tttctttttt caggctattg ccgtgcaaat
     1801 caagaatgta aatttcatcg aaggttttca ttagactttg acgcattccg cgaaaagtag
     1861 gattgtccag ataactgtga ttggtaatca tccccacgat gccgtaaccg gattgctgga
     1921 ttttccattg cgcgaagcgt agaaatttga cataatcgtc ctgcaaccat ttaggattcc
     1981 tttctcccag cggctgacca tctactttgt aataactctg ggcgccgtca aggtcttctt
     2041 tgagcagtcg ttccgtccat ttattgatat tggcggaaat gccgctataa ggtggattac
     2101 caagaatcac taggatcggc tgttctttct ttactttttc cgcaaaatga ctttcttcac
     2161 tcagcgaaga aagtaaggga attgtaattt cctttatttc ttccatttcc agcgtgttgg
     2221 tcagataaag tttgaagcgt tcatcctcag ccattttgta accgagttcg tcaagaataa
     2281 aaccaatttt caagtgaccg atggcatagg gcgccatcat cagctcaaaa gcgtgaaaat
     2341 tggcgagaat atgtttcttt atccataaat gtaaaccgcc ttcgccgtat ttgcttttga
     2401 attcatcagc cgccagccgg atggcttcgg cgggaaaggt gagcgtacca ccggctgggt
     2461 cgagcagttt tacttcttca ctggcgagac cgtccgccag attaaaatgc gattttaaaa
     2521 tggaatgaat cgaacgaacg atatatccga ccaccggttc cggggtataa tatactccgc
     2581 gccgttcacg gattttagga tcgtaggtag ccagaaaagt ttcataaaaa tggataattg
     2641 gatcccggcc ttttccggcg cgctggtatt catgcaagat tttattgacg tcggcaacat
     2701 ttagaatttc gacaatatcg tccacgatga tttggagtga tttcggagga tctccgagcg
     2761 agataaagtg gaatatatcc cgcaaaattc cgatagtatg gggaatataa tcgaaggcaa
     2821 ttttacgatt aaattcatcc cgctcagatg taactcttgt ccgagcggcg aacaacccgt
     2881 atgtgatagt ttgagcataa agatcggcga attgttctgc ggttaaagtc ggtatcaggt
     2941 aagttttaaa ggcttcataa aagcccgcta tttgcttctg acctttgctc ccattttccg
     3001 ccatttcgac tgcaattacc tcatcgcgca agaatcgggt gcgtttggct aactcgactg
     3061 ccagcgaacg cgcattttga actttgggca gagaaaagga aaagaataaa tcaaagagtt
     3121 caatgaattg ctcgatattt tccagcggcg gtttggtttt tagcttctgg gcaattaccg
     3181 gtcgtccaat cataactcgc cggattcgct ctccgtcccg ataaagtcgg aattcataaa
     3241 aattagttaa gataacattg ggaaaggtgg atatatagcg ttttaattgt tcggaatctt
     3301 cgatatagtc cagatcggta atcgaagggt ctttggcttc gatgtaaccg gtgatgtggt
     3361 tttgaccgtc ccaaactcgg aagtcaggat ttccggcatc agtctttttg ggaagaatcg
     3421 ttacttcaat atttttaatg ttttgaattt ccgcgcactg tttaatcagg tcatcgagat
     3481 gtttgtaata actttcctcg cgggcatcac cccgattgaa agtatctgat agattcgcta
     3541 aatatttttc tagtatctgt ttcacttaac cagaatgttt tttatatgtt aaatgacaat
     3601 ttaggcgata ttgcaagtaa aaatattttc cagatatttc caaattagtg ctataatttt
     3661 tcaatgttag gttttgagat ttttacgatt tttctccatt caataatcgt ttgtaatttt
     3721 agaaaattta tgagaataaa caggtcttga tattgctatt taagttgctt tttcctctta
     3781 taatataaaa caatttactg ccatatttcg tagcaaaaca atattaggta aatcttcagt
     3841 taaagtcaat gtgttttaat tacaggtttt ttcccatttt tattgaaagt ttaacctcta
     3901 cagggtaaat aatttttttc aataaaataa tttctacaat actttaaaac ctccggtcta
     3961 ttttggattc tattgtcgtt agattggcac ccgccgcttt gtggtcaaga tattgtagag
     4021 tgttgtgttc ggtagcgatt cgtagtcatg cagtagtgat tatggatacc gctcagcggt
     4081 taaggtatta tagaccaata gaaatcgaaa agctattccc tatatgggat aaaggagact
     4141 atttttcaac tttttaacgc ttttttccct ccggaatatc tacctgcttg tcgtctaacc
     4201 actatttcaa ctggaagaat ccggtatccg gcttgaaaag ttaaattata ccgattttta
     4261 gaaggctaat agaaattaga taggaggttc taaattattt aaacatttgt attttaaggt
     4321 gtaccccaac aaggactcga accttgaacc aacggattaa gagtccgctg ctctaccatt
     4381 tgagctattg gggctgaaaa aaagcgggtt aatttaagat tttctggaga atcaaacaaa
     4441 atagcaggca tcattcgatt cgctaagctt gattgtatcc tgacttaatt ttaagttaag
     4501 ttcctaatga aatgtatttc ttttatattg aataaattga ccgctaa
//
LOCUS       DAOMJU010000116         4532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_20800_length_4532_cov_25.299531, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000116 DAOMJU010000000
VERSION     DAOMJU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20800_length_4532_cov_25.299531"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1497)
                     /locus_tag="PLO58_08845"
     CDS             complement(16..1497)
                     /locus_tag="PLO58_08845"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HQO74935.1"
                     /translation="MGKQVSNEQMDKLVSLCKRRGIIFQSSDIYGGLSSTWDYGPLGV
                     ELKRNVKDLWWHDMVTSRENVVGMDAAILMHPKVWEASGHVANFHDPLIDCKKCQARF
                     REDKAPAYFKCSNCNFNFYVNDGLIEVKKEFAAHFPDLYAREIAHYRDSDCPHCGGQR
                     TIKLEKIACPNCGGSELSPARQFNLMFKTHFGPSEDSASEVYLRPETAQGIFVNFDNV
                     ISTTRVKLPFGVGQIGKAFRNEITTGNFIFRSREFEQMEMEFFVKPEESGRWYEYWVN
                     ERFNWYKKLGVRAENLRRRPHEKEELAHYAKACTDVEYKFPFGWSELEGIADRQTFDL
                     EQHMKASGKKLTYFDSETNTHIVPAVVESSAGVDRTILTVLCDAYWEDAEHDRVVLRL
                     DPKIAPTKVAILPLVNRDGMPEIAEAIFNDVKKQFAAFYDQAGSIGRRYRRQDEAGTP
                     FGITVDSQTLQDGSVTLRDRDTLEQIRLPKENIVKVLQEKLAL"
     gene            complement(1579..3786)
                     /gene="topA"
                     /locus_tag="PLO58_08850"
     CDS             complement(1579..3786)
                     /gene="topA"
                     /locus_tag="PLO58_08850"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQO74936.1"
                     /translation="MELLIVESPSKAKTIKKYLGNNFKVMASVGHIKDLPEKELGVDI
                     QHDFKPKYVTIKGKSKIISNLKESAQSAQRVYLAPDPDREGEAIAWHIANVIDVPSDR
                     VQRVLFNEITESGIRYGLSHPRQIDMSLVNAQQARRVLDRLVGYQVSPFLWRTLYSGL
                     SAGRVQSVAVRIICEREVEIEQFIPLEYWEITALLQTPQNEKFSAKLIKIDNKKTAIS
                     NASDANKHLAILKNATYQVSNIEEKEVTKSPAPPFTTSTLQQEATRRFRYSPEKTMRI
                     AQNLYEGVEIGGEPVGLITYMRTDSVRISKEAITGIREYIEKHYGAKFLPPNPRHFKT
                     TKRNVQDAHEGIRPTHFEYTPEVVAPYLSPEQKKLYTLIWNRFAACQMSAAVYKQKII
                     DVTADNYLFRATGSDLIFDGYLRAWREEKAEDEKKIALPINIQKDDRLTLLELQPEQK
                     FTEPPPRFTEGTLIKELDNLGIGRPSTYATIVSTIITRKYVERKGGYLVPTDLGKTVN
                     KLLVDGMPDIFNVKFTARMEAELDDIEGNRKEWQKVISEFYQPFHKSLEELQAQRREI
                     KTVLQEKTNEKCEICGAPMVIKWSRNGRFLACSAFPECKNTRPLNTAPVAPTESKTCP
                     NCGSPMTIKDGRFGKFWACTKYPKCKTTEPFTLNIKCPEPDCDGQIVQKRTKKGKIFY
                     GCNRYPNCKFATWNEPVARPCQNCDSQFLERKIKRDGSVLLICPKCKTETADE"
     gene            4342..4415
                     /locus_tag="PLO58_08855"
     tRNA            4342..4415
                     /locus_tag="PLO58_08855"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4376..4378,aa:His,seq:gtg)
     gene            4429..4503
                     /locus_tag="PLO58_08860"
     tRNA            4429..4503
                     /locus_tag="PLO58_08860"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4462..4464,aa:Glu,seq:ttc)
BASE COUNT         1157 a          994 c         1033 g         1348 t
ORIGIN      
        1 aacctcgacg gttccttaca acgctaattt ttcctgcagt accttgacaa tattctcttt
       61 gggaagacgg atctgctcga gtgtatcgcg atcgcgcaat gttacactgc cgtcttgtaa
      121 agtctgcgaa tccacggtaa ttccaaacgg cgtcccggct tcatcctggc gccgatagcg
      181 tcgtccgatg gaaccggctt ggtcataaaa agccgcaaac tgttttttta catcgttaaa
      241 aatggcttcc gcgatttccg gcattccatc acgattgacg agcggtagga tagcgacttt
      301 ggtcggagct attttcggat cgagtcgcaa gacgacgcgg tcatgctcag catcttccca
      361 gtaagcatca cacaaaaccg tcagaatggt gcgatctacg ccagcagaac tttcgacaac
      421 ggctggtacg atatgcgtgt tcgtttcgct atcaaaataa gtcagcttct tgccgctggc
      481 tttcatgtgt tgctcaaggt cgaaagtttg ccgatcggcg atgccttcca gttccgacca
      541 gccgaaggga aatttatatt caacatcagt acaggctttg gcataatggg ccagttcttc
      601 tttttcgtgc ggacgccgcc gcaaattttc cgcacggaca ccgagttttt tataccagtt
      661 aaagcgttca ttgacccaat actcgtacca gcgcccgctc tcttccggtt tgacaaaaaa
      721 ttccatttcc atttgttcaa attcgcggga gcgaaaaatg aaattgccgg tcgtgatttc
      781 attgcgaaaa gcctttccga tctgaccgac accaaacggt agtttgacac gcgtagtgga
      841 aatgacatta tcgaaattga caaaaatgcc ctgcgccgtt tccggccgta aatagacttc
      901 agaagcagaa tcttccgatg gaccaaagtg ggttttgaac attaaattga attggcgggc
      961 gggtgatagt tccgaaccgc cgcagttcgg gcaggcgatt ttttccaatt tgatggttct
     1021 ctggccgccg caatggggac agtcggaatc gcgataatgc gctatttcgc gagcgtataa
     1081 atcgggaaaa tgcgccgcga attccttttt tacttcaatt aaaccatcat tgacgtaaaa
     1141 gttaaaatta caattggagc atttaaaata ggctggagct ttatcctccc ggaaacgcgc
     1201 ctgacatttc ttgcaatcaa tcagtgggtc atgaaaattg gcgacgtgcc cggaggcttc
     1261 ccagactttg gggtgcatca atatagcggc gtccataccg acgacatttt cccgactggt
     1321 gaccatatca tgccaccaca aatctttgac attacgctta agttcgacac cgagcgggcc
     1381 ataatcccag gtactgctca gcccgccata aatatcactg gattgaaaga tgatgccgcg
     1441 ccgtttacat agcgagacta atttatccat ttgttcgtta ctaacttgtt ttcccatttt
     1501 ttccctaatt ttcacatttc aatatttaat ttacaaatag ttcagaagga aatttctaat
     1561 taagcaacat gtgatttttc actcgtctgc tgtttcagtt ttgcattttg ggcaaatcag
     1621 cagaacactg ccatcgcgtt taatcttgcg ttccaaaaat tggctatcgc agttctggca
     1681 ggggcgagcc accggttcat tccaggtagc gaacttgcag ttcgggtagc gattacatcc
     1741 gtaaaaaatt ttgccttttt tggtgcgctt ttgaacgatt tgcccgtcgc aatctggttc
     1801 cgggcatttg atattcaaag tgaatggttc ggtcgtttta catttaggat atttggtgca
     1861 agcccagaat ttgccgaaac gcccatcttt gatcgtcatc ggactgccgc agttggggca
     1921 ggttttggat tcggtcggcg caaccggcgc ggtatttaac ggccgggtgt ttttacattc
     1981 gggaaaagcc gagcaggcta aaaaacgccc attgcgactc catttgatga ccatcggagc
     2041 gccgcagatc tcgcattttt cattagtttt ttcctggagt acggttttga tttcgcggcg
     2101 ctgggcttgg agctcttcga gcgatttatg aaaaggctga tagaattcgc tgatgacctt
     2161 ttgccattct ttacgattgc cttcaatgtc atccagttcg gcttccattc gagcagtgaa
     2221 cttgacattg aaaatatcag gcataccgtc cacgagtaat ttattgacag ttttgcctag
     2281 atcggttggc accagatagc cacctttacg ttcaacgtat ttacgggtaa taattgtcga
     2341 aacgatggtg gcataagtgc ttgggcgacc gataccgaga ttatctaact ccttgataag
     2401 cgtaccttcg gtaaagcggg gcggcggttc ggtaaatttt tgctccggtt ggagctctaa
     2461 caaggtgagc cggtcgtctt tttgtatatt gatcggcaga gcaatttttt tctcgtcctc
     2521 ggctttttcc tcgcgccagg cacgcagata gccgtcaaaa atcaaatcac tgccagtagc
     2581 gcgaaaaagg taattatcag ctgttacatc aattattttt tgtttgtaaa cagccgcaga
     2641 catctgacag gcggcgaagc gattccaaat tagagtataa agctttttct gctcgggcga
     2701 aaggtaggga gcaactactt cgggcgtgta ttcaaaatgc gtggggcgaa ttccttcatg
     2761 cgcgtcttgc acgtttcttt tagtagtttt aaaatggcgc gggttaggtg gaagaaactt
     2821 tgcgccgtaa tgtttttcga tatattcacg gataccggtg atagcttcct tagaaatacg
     2881 taccgaatca gtgcgcatgt aggttatcaa gccgaccggc tcaccaccaa tttcgacacc
     2941 ttcgtaaaga ttctgagcaa tgcgcatagt tttttccggc gagtagcgaa aacggcgcgt
     3001 ggcttcctgt tgcagggtgc tggtcgtgaa aggtggcgcg ggtgatttgg tgacttcttt
     3061 ctcttcgata ttgctgactt gataggtagc atttttcaga atcgctaaat gcttgttggc
     3121 gtcggatgcg ttcgaaatag cagttttttt attgtcaatt ttaatgagct tggcagaaaa
     3181 tttttcgttt tggggtgtct ggagcagtgc cgttatttcc cagtattcaa gaggaatgaa
     3241 ctgctcgatt tcgacttcgc gttcacaaat gatacgcacc gcgacggact gcacacgacc
     3301 ggcgctcaat ccactataaa gtgtgcgcca tagaaaaggg ctgacttggt aaccgaccag
     3361 ccgatcgagg acccgtcggg cttgctgggc attcaccagg ctcatatcta tctggcgcgg
     3421 atggctaagt ccataacgaa tgccactttc ggttatttca ttgaaaagaa ctcgttgaac
     3481 gcgatcggat ggaacgtcaa taacattggc tatatgccag gcaattgctt caccttcgcg
     3541 gtctggatca ggggctaaat aaacacgttg agcagattga gcggactctt tcagattact
     3601 tataatttta gatttgcctt tgattgtaac gtatttcggt ttgaaatcgt gttgaatatc
     3661 aacaccgagt tctttctccg gtaaatcttt gatgtgacca accgaagcca tgactttaaa
     3721 attgttaccg aggtattttt tgatagtctt ggctttagat ggcgattcga cgattagtaa
     3781 ttccatatca ctttactttt cttaaggcgg ggtaaattac aaatactgat cagaattcca
     3841 agcattttta aaataaaact tgttcatttc aaaaatactc actgatattg agacttccac
     3901 aaattttaag aaaaataccc gcgctttttg attatcagat tagttttcgc ctcatcatcc
     3961 aattttgttg aatcacgaaa aaaagatgtt tatttccacc gctgaaagta ataatagtga
     4021 attcagaaat accgaatcct gttttaggaa aaggctaaaa tatgaacgca caaaaactga
     4081 cctgcacgtt ttaagatagg taagcacaat agattttaat aatttttaaa aaaatgtaat
     4141 ttaatatcca gttaaagtaa tatcattctg attatcagca tattagcaga taagttcggg
     4201 aaatattagg ggatatcctg gtgcttggtg tattgacatt ctgattataa ataacttaaa
     4261 tttcacagct tttttaaaat aattgatcca ttcatgaaat tgatctgggc ttactaacct
     4321 cctgcgggag tgatatagat ggtgagtgta gctcagtatg gtagagcgac aggttgtggc
     4381 cctgttggcc gcgggttcaa gtcccgtcac tcacccgctc tttttaatgg cgctatcgtc
     4441 tagtggttag gacaccacgc tttcaatgtg gtaaccggag ttcaattctc cgtagcgcta
     4501 ccaatcgttc caagtctttt ctttagcctt ta
//
LOCUS       DAOMJU010000117         4514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_20927_length_4514_cov_22.724378, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000117 DAOMJU010000000
VERSION     DAOMJU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20927_length_4514_cov_22.724378"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLO58_08865"
     CDS             complement(<1..723)
                     /locus_tag="PLO58_08865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQO74937.1"
                     /translation="MLKLKQIGIITVLILMNTVHAGEINPSYEVGIWQGFRSAAVTFT
                     FDDGCANQFTVAIPLFDQHGFKLTLYPVINWSGGIWSKLQSAAANGHEVGSHTVTHPD
                     LSGLSEAEQITELQNSQNEINSRISGQRCITIAYPYCNAGKTSICKQYYIAARGCSGQ
                     IEPKTPSNFMNISSIVCGTEGSVKTANDFFNKAKSAANSKGWVVFLIHGIDNDGGWSP
                     VTTENLTGAVDSLSAYPDKFWVS"
     gene            complement(846..>1254)
                     /locus_tag="PLO58_08870"
     CDS             complement(846..>1254)
                     /locus_tag="PLO58_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 65"
                     /protein_id="HQO74938.1"
                     /translation="RKDHPTLLASYGMLADKTVDQKVMEKTLEKVMQSWDWETTWGWD
                     FPLIAMTAARVGRADLAIEALLMDVPKNTYLLNGHNYQNESLPVYLPGNGGLLTAVAM
                     MAAGWDGAPDIYAPGFPQDGQWSIKYENLVPLP"
     assembly_gap    1255..1354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1355..2460)
                     /locus_tag="PLO58_08875"
     CDS             complement(<1355..2460)
                     /locus_tag="PLO58_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74939.1"
                     /translation="MGKNRQKYDKKRNLNELLAIVVFLSSFVVFFSCENNAKIDREAL
                     VKRHSPTLNASDSLNPFTVGNGEFAFTTDFTGLQSFPEYYEATTPLVTQAEWGWHSFP
                     NVNQYSLDQVYEKYEVNGRKVAYASHQDSEAGEYLRSNPHRLHLGQIGFKFKNQNGLP
                     IRINEIRNIKQTENIWEGIIYSSFEVLNTMMEVETACHPVYDQIAVRIKSALLNQGKV
                     AISFNFPYGSSRWGKNPADWYSPEKHISAIVSESPNSVLIKRTLDTTEYCVKIYWRGD
                     AEFTKTDRHSFNLAIKSGKNFEFTCLFLERESGQGSASVTQTFSAARSFWQNFWETGG
                     AIDLSASKDSRAKELERRIILSRYLTRIQCAGSLP"
     gene            complement(2663..>4514)
                     /locus_tag="PLO58_08880"
     CDS             complement(2663..>4514)
                     /locus_tag="PLO58_08880"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HQO74940.1"
                     /translation="VLLLVLLALTVNAQNFSITIDAEKDAFYSELTGPSNGLVFMPYL
                     CYLRDIGTPPTDNADLSAKVWFSYDADYLYCYAEVKDDIVAVTNTERFQNDCMELKFD
                     PDPNSGTNTKTANCRITAKGADNAEEPTGVDNLNGSGHLEDVEGTDYVVSESDYARRL
                     TDDGYVLEFRVPFDYINEPEDNRFMVERTVGNKFGMAINIGDNDGTTREHMIQWSAGH
                     TDGAHSYPALCGTATFMDNHVLKLEAVGPRPQDTVVVNPNAGWYIDPNFAVAIDAEKD
                     AFYSELTGPSNGLVFMPYLCYLRDIGTPPTDNADLSAKVWFSYDADYLYCYAEVKDDI
                     VAVTNTERFQNDCMELKFDPDPNSGTNTKTANCRITAKGADNAEEPTGVDNLNGSGHL
                     EDVEGTDYVVSESDYARRLTDDGYVLEFRVPFDYINEPEDNRFMVERTVGNKFGMAIN
                     IGDNDGTTREHMIQWSAGHTDGAHSYPALCGTATFMDNHVLKLEAVGPRPQDTVVVNP
                     NAEEWYTKDPTVAIKVEPSVAKSFNLLTNYPNPFNPSTTIKFSVPKTALVTLKVHNTL
                     GQEVAQLVAQRLNEGVYTVNWNAAEFPSGIYIVRMVAGDLINTSKMILLK"
BASE COUNT         1268 a         1047 c          831 g         1268 t
ORIGIN      
        1 ggatacccag aatttatccg gataggcact tagagaatca accgcaccag tcagattttc
       61 cgttgtcact ggcgaccaac cgccgtcgtt atcaatgcca tgaattagaa aaacgaccca
      121 gcccttggag ttcgccgcgc ttttggcttt attaaagaaa tcgttagcgg tttttactga
      181 accttctgta ccgcatacga tagagctgat attcataaaa ttgctcggag tttttggttc
      241 aatttgcccg gagcaaccgc gggcggcgat gtagtattgc ttgcaaatcg aagttttacc
      301 ggcattacag tagggataag ctattgtgat gcatcgctgt ccgctgatgc gagagttaat
      361 ttcattttgc gaattttgta attctgtgat ttgttccgcc tccgataaac cgcttaaatc
      421 agggtgagtt acggtgtgac tgccgacttc atggccgttt gccgcggcgg attgcagctt
      481 tgaccaaatt ccacccgacc agtttattac cggatataac gtgagtttga atccatgctg
      541 gtcaaagagt ggaatagcca ccgtaaactg gttggcgcaa ccatcatcaa aggtaaaagt
      601 gaccgccgcg gaacgaaatc cctgccagat tccgacttca tagctcggat ttatttcccc
      661 ggcgtggacc gtgttcatta aaattaatac tgtgatgata ccaatttgtt tgagcttcaa
      721 cataaattac tcctgtatta aataaggcta aatttcaaat ttttgaaccg agaaatatta
      781 tttatttttt tatccttaat gcaaatttta tttaacctac ttatcttaga aattctcata
      841 atgttttatg gtaaaggcac taagttttca tatttaatag accattgacc atcttgcgga
      901 aatccaggag cataaatatc aggagcgcca tcccaaccag ccgccatcat cgccacagct
      961 gtcaataaac ctccgttgcc aggcaaataa accggtaagg attcattttg ataattgtga
     1021 ccattcaaaa ggtaagtatt tttgggtaca tccatcagta aagcctcgat cgccaaatcc
     1081 gctctgccta cccgagccgc cgtcattgct atcaagggaa aatcccagcc ccaagtcgtt
     1141 tcccaatccc aggattgcat aaccttttca agcgtttttt ccataacctt ttgatcaaca
     1201 gtcttatcgg ctaacattcc ataggaagcc aataaggttg ggtgatcttt cctannnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcaaa cttccggcgc attggatacg
     1381 agtcaaataa cgtgatagga taattctgcg ctctaattcc tttgcccgtg aatctttact
     1441 tgccgaaaga tctatagctc cgccggtttc ccagaaattt tgccaaaatg atcgtgcggc
     1501 ggaaaaagtc tgggtaacgc ttgcggatcc ctggccactt tctctttcta aaaacaggca
     1561 agtgaattca aaatttttac cggatttgat tgccagatta aacgaatgtc ggtcggtttt
     1621 tgtaaactca gcatctcctc gccagtaaat tttgacacag tattctgtgg tatctaaggt
     1681 cctcttgatt aaaactgaat tagggctttc cgaaacgatg gcggaaatat gtttttcggg
     1741 gctgtaccaa tcggctggat ttttccccca tctcgacgaa ccgtagggaa aattaaaact
     1801 tattgcaact tttccttgat tcaacaatgc cgacttgatt ctgaccgcaa tttgatcata
     1861 taccggatgg caggcggtct ctacttccat catagtattc aagacttcaa aggaactgta
     1921 aattatcccc tcccagatat tttcggtttg cttgatgttg cggatttcat ttatcctaat
     1981 cggcaagcca ttctgatttt tgaatttgaa gccaatttgt cctaagtgaa ggcgatgcgg
     2041 atttgagcgt aaatattctc ctgcttctga atcttggtgc gaggcatagg ctactttccg
     2101 cccgttaact tcatattttt cgtaaacttg atccagtgaa tattgattta cattgggaaa
     2161 agaatgccaa ccccactccg cctgagtcac taacggggta gtcgcttcat aatattctgg
     2221 aaaactttgt aatccggtaa aatcggtagt aaaggcaaat tcaccgtttc ctactgtaaa
     2281 aggattcaga gaatcgcttg cgttcaaagt gggactatga cgtttgacca acgcctcgcg
     2341 gtcaattttc gcattatttt cacaggaaaa aaatacgacg aaagaactta agaaaacgac
     2401 aattgccaat aattcgttca ggtttcgttt tttatcgtat ttttgtctat tttttcccat
     2461 tacaaatgcc atcaaaaaca agaggttgtc ttaaaagccc cattgtgttt tccccttttt
     2521 ggtcattgct atcttaacga ccccgcgcct gcttagccaa aatctctact tcgtcaaaaa
     2581 tccaggatac aatttccaca aaggcgctat gaaacacaaa gtaaaaaaac taataagaca
     2641 acctctaatt ttttgcgtcg gtttacttaa gcaaaatcat cttgcttgta ttgattaaat
     2701 cacccgcaac catccgaacg atataaattc cactcggaaa ttcagcggca ttccaattaa
     2761 cggtatagac accttcattc aatctctgcg ctaccaattg agcgacttct tgacctaagg
     2821 tattatggac tttcaaagtg accaaagccg tcttaggaac ggaaaactta atcgtagtgc
     2881 ttggattaaa tggattcgga taattagtta aaagattaaa agacttggct acagacggtt
     2941 caactttaat agctacagtt ggatcttttg tgtaccattc ttcggcattg ggattcacta
     3001 ccacggtatc ctgaggacgt ggtccaacag cttctaactt caatacatga ttatccataa
     3061 atgtcgccgt tccacaaagt gccggataag aatgagctcc atcggtatgc ccagctgacc
     3121 actgaatcat atgttcacgg gtagtcccat cattatcacc aatattgatc gccatcccaa
     3181 acttattccc taccgtgcgc tctaccataa accgattatc ttccggctca ttgatgtaat
     3241 caaacggcac ccgaaattca agaacatagc catcatcagt caatctacgg gcataatccg
     3301 attccgaaac aacataatcg gtaccttcaa catcttccag atgaccagaa ccgttcaaat
     3361 tatcaactcc agttggttcc tcagcattat cggcgccttt agcggttatc cgacaattcg
     3421 cggttttcgt attggtaccg ctgttcggat caggatcaaa ttttaactcc atacagtcat
     3481 tctggaacct ctctgtattg gtaaccgcta cgatatcatc ttttacctca gcataacaat
     3541 agaggtaatc ggcatcataa gaaaaccaga ctttagccga gagatcagca ttgtcagtcg
     3601 gtggcgtccc aatatcccgc agatagcata aataaggcat aaacaccaac ccgttcgacg
     3661 gaccagttag ttctgaataa aatgcgtcct tctcagcatc aatcgcaact gcgaaattag
     3721 gatcaatata ccaaccggca ttgggattca ctaccacggt atcctgagga cgtggtccaa
     3781 cagcttctaa cttcaataca tgattatcca taaatgtcgc cgttccacaa agtgccggat
     3841 aagaatgagc tccatcggta tgcccagctg accactgaat catatgttca cgggtagtcc
     3901 catcattatc accaatattg atcgccatcc caaacttatt ccctaccgtg cgctctacca
     3961 taaaccgatt atcttccggc tcattgatgt aatcaaacgg cacccgaaat tcaagaacat
     4021 agccatcatc agtcaatcta cgggcataat ccgattccga aacaacataa tcggtacctt
     4081 caacatcttc cagatgacca gaaccgttca aattatcaac tccagttggt tcctcagcat
     4141 tatcggcgcc tttagcggtt atccgacaat tcgcggtttt cgtattggta ccgctgttcg
     4201 gatcaggatc aaattttaac tccatacagt cattctggaa cctctctgta ttggtaaccg
     4261 ctacgatatc atcttttacc tcagcataac aatagaggta atcggcatca taagaaaacc
     4321 agactttagc cgagagatca gcattgtcag tcggtggcgt cccaatatcc cgcagatagc
     4381 ataaataagg cataaacacc aacccgttcg acggaccagt tagttctgaa taaaatgcgt
     4441 ccttctcagc atcaatcgta atggaaaaat tctgagcatt tacggtcaat gctaacaaaa
     4501 caagaagcaa aacc
//
LOCUS       DAOMJU010000118         4483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_21137_length_4483_cov_17.470190, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000118 DAOMJU010000000
VERSION     DAOMJU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21137_length_4483_cov_17.470190"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..1889
                     /gene="pruA"
                     /locus_tag="PLO58_08885"
     CDS             258..1889
                     /gene="pruA"
                     /locus_tag="PLO58_08885"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HQO74941.1"
                     /translation="MSNAKFTLPQPVNEPQFSYLPGSSERQALKIALNKIAGEQIEIP
                     LIIGGKEIRTGQTAKCIMPHNHQHILAVYHQATAKEVSLAVEASQKAWEFWSVMPWHE
                     RAAIFKKAAALLSGPWRMTLNAATMLNQSKNVFQAEIDSACELIDFLNFNVKFMEQIY
                     EQQPLSSPGQWNRLEQRPLEGFVFAVTPFNFTSIAGNLPTAPAIMGNTVLWKPASTAV
                     LSAYYFMKLLQEAGLPDGVINFIPGTGRNVGPNVLSNSLLAGVHFTGSTAVFQSMWKT
                     IGENIANYRGYPRIVGETGGKDFIFAHVSADLDELNTAIIRGAFEYQGQKCSAASRVY
                     IPKSLWPPLKEKILASLSTIKIGDVRDFRNFINAVIDKAAFDNILSYINYAKNSRDAE
                     IIAGGKGDATNGYFVEPTVIITTDPHFKTLEEEIFGPVMTVFVYDDNKYEETLNLCNM
                     TSPYALTGAIFARDRQAILLADKILLHAAGNFYINDKPTGAVVGQQPFGGGRASGTND
                     KAGSMINLLRWVSPRATKELFVPPKDFHYPYMIEE"
     gene            complement(1955..>2665)
                     /locus_tag="PLO58_08890"
     CDS             complement(1955..>2665)
                     /locus_tag="PLO58_08890"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HQO74942.1"
                     /translation="EKALRAIEILHRLDESSQPDQEFVKYQGAYIRKSALKGAIQNYE
                     LIPKIFYGNLIMQKIGSIDNLTWDKVKNIFTGEADKSAENWEDLSGMFATSAEISKIN
                     LALRENRLNSIVDLECALRDVHQNYEENTWQFCLNLLFKRFTTNALNSEQVCQLLDEW
                     QASVVRFNNLVLKDAEKEFAQCSRVGYGIDGDAIIRDQDFEAVRGTFAENKFVVELKK
                     ESEEAVKKVERICAALRK"
     assembly_gap    2666..2765
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2766..3526)
                     /locus_tag="PLO58_08895"
     CDS             complement(<2766..3526)
                     /locus_tag="PLO58_08895"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HQO74943.1"
                     /translation="MENRQLTQAEINLLEKQGCRAENWEIITVKPDFNPANIHNVTFA
                     GAVEIGNCCGVQEVKPGCLKNCGIYDSYIRNCTIADNVYIANVKNLINYTIEKDVIIE
                     NVGDLIVTGETTFGNGTELEILNEGGGRILPIYDRLNAQIAYMIVVYKHDANLQKALK
                     KLVSDYVAKRKANRGVISQGVRIYNSNIIRNVQIGEKATVSGALLLEDGTIAGSEVDP
                     VYIGEGVIAKHFIIQSGVRVDSSAVLDKCFVGQGTR"
     gene            complement(3542..4348)
                     /gene="nagB"
                     /locus_tag="PLO58_08900"
     CDS             complement(3542..4348)
                     /gene="nagB"
                     /locus_tag="PLO58_08900"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQO74944.1"
                     /translation="MRVIIHNNYEKLSDWVARYVAAKIIHANQTSAKPFVLGLPTGSS
                     PIGTYQALVRLYKEGKVSFKNVITFNMDEYVGLSEDDPESYHYFMRHYLFDHIDIPPE
                     NINILDGNAKDLEKECEEYEAKIKRVGGIDFFLGGIGVNGHIAFNEPGSSISSRTRIK
                     TLSYDTIVANSRFFGGDLSRVPKTALTVGVGTVLDAREVMVIASGYTKARALQKVIEE
                     GVNHMWTVSMLQLHPHGTIVCDDEATLELKVGTVEYFKDIEDEAINNLPV"
BASE COUNT         1259 a         1013 c          801 g         1310 t
ORIGIN      
        1 caactttaaa tattcaacct cataattttc gcaaaaatca ctatgaaaaa tatgtattat
       61 aaagccaaac aatggcgatt aatactgtat tcgtcgtcat aaacaacttc atatttccaa
      121 aattccacta aaatataaat ttttactact taacaacgtt taatcctacc aattcagaaa
      181 atagtttatt aatttaagtc agtgttaagc attttaacat tgtatcccaa gttcaacgcc
      241 agagaggaga gttatcaatg agtaacgcca agttcacctt accccaaccc gttaatgaac
      301 cgcaattttc ctatttgccc ggcagttcgg agcgccaggc tttaaaaatt gctttaaata
      361 aaatagccgg cgaacagatt gaaatcccgt taattatcgg cggcaaggaa atccgcaccg
      421 gacaaaccgc gaaatgcatt atgcctcaca atcatcagca tattctggct gtctatcatc
      481 aggcaactgc caaagaagtt agcctggcag tcgaggcttc tcaaaaagcc tgggaatttt
      541 ggtcagttat gccctggcac gaacgcgccg caattttcaa aaaagccgcg gcattgctat
      601 ccggtccttg gcgtatgacg ctcaatgcgg cgacaatgct caatcaaagt aaaaatgttt
      661 tccaggcaga aattgattca gcgtgcgagc taatcgattt cctcaacttt aatgtcaaat
      721 ttatggaaca aatctatgag caacagccgt tatcatcacc tggtcaatgg aaccgattgg
      781 aacagcgtcc cctggaaggt tttgtttttg ccgtaacgcc gttcaatttt acttccatcg
      841 ccggtaattt gccgactgct ccagctataa tgggcaatac cgttctctgg aaaccagcca
      901 gcaccgcggt gctctccgct tattatttta tgaaattatt acaagaagcc ggactgccag
      961 acggtgtaat taactttatc cctggaaccg gtcgcaatgt aggaccgaat gttctttcta
     1021 attcgctact ggcaggtgtg catttcaccg gctcgacggc cgtttttcaa agtatgtgga
     1081 aaactattgg tgaaaatatt gccaattacc gtggttatcc acgcatcgtc ggggaaaccg
     1141 gcggtaagga tttcatcttt gctcacgtca gtgccgatct ggatgaactt aataccgcga
     1201 ttattcgcgg cgcttttgaa tatcaaggcc aaaaatgttc ggcggcttct cgggtttata
     1261 ttcctaaatc gctctggccg ccattaaagg aaaaaatact cgcttccctt agtacgataa
     1321 aaatcggcga cgtgcgcgat ttccgcaatt ttatcaatgc tgttattgac aaagccgctt
     1381 tcgataatat tttatcgtat atcaattacg ctaaaaattc ccgcgatgcg gaaataattg
     1441 ctggtggaaa aggcgacgct actaacggct atttcgtcga gccaactgtt ataataacga
     1501 ccgatccgca tttcaaaact cttgaagaag aaatattcgg cccggttatg accgtttttg
     1561 tctatgacga taataaatac gaagaaactc tcaatctctg taatatgact tcaccgtacg
     1621 ccctgaccgg cgccattttc gcccgtgatc ggcaggctat cctgcttgcg gacaaaattc
     1681 tactccacgc cgccggtaat ttctatatca atgataaacc aaccggtgca gtcgtcggtc
     1741 aacaaccatt cggtggcgga cgtgcttccg gtacgaatga taaagccggt agtatgatta
     1801 accttttgcg ttgggtttca ccacgcgcga cgaaggaact tttcgttcca ccaaaagatt
     1861 ttcactatcc gtatatgatc gaagaataaa ttaataagcc cgtgaattaa ttcatgggca
     1921 tgttattagt gagtttaatg tttttgaggc ttttttattt tcggagagcc gcgcaaatac
     1981 gctcgacttt ttttacggct tcttccgatt cctttttaag ttccaccacg aatttatttt
     2041 ctgcaaaagt ccctcgcacg gcttcaaagt cctgatcacg gataatcgca tcaccatcaa
     2101 ttccataccc gactcgactg cattgagcaa attccttttc agcatctttg agaaccagat
     2161 tattaaagcg aacgacactt gcctgccatt catctagcaa ttggcaaacc tgttcggaat
     2221 tcagggcatt ggtcgtaaaa cgtttaaaga gcagattgag acagaattgc caggtatttt
     2281 cttcataatt ttggtggaca tctcttaaag cacattccaa atcaacaatt gaattaaggc
     2341 gattctcccg cagtgctaaa ttgatcttac ttatttccgc gctggtagca aacataccgc
     2401 taagatcttc ccaattttct gccgatttat ccgcttcacc agtaaaaata tttttcactt
     2461 tatcccaggt taaattatcg atcgaaccaa ttttctgcat aatcaaattg ccatagaaga
     2521 ttttcggaat taactcatag ttctggatag ccccttttaa ggctgacttt cgaatatacg
     2581 caccttgata tttaacgaat tcttgatccg gctgtgaaga ttcatcaaga cgatggagta
     2641 tttcaatagc gcgaagagcc ttttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnncgcgt tccctgacca acaaaacatt tgtcaagcac tgcagaactg tccactcgca
     2821 cgccgctttg tattataaaa tgtttagcga ttacaccttc tccaatgtaa accggatcca
     2881 cctcagaacc ggcaatggtt ccatcttcga gtagtaatgc tcctgaaacg gtagcttttt
     2941 cccctatttg cacgttgcgg atgatattac tgttgtaaat tctgactcct tgagaaatta
     3001 caccacggtt cgctttgcgt ttagcgacat aatcgcttac cagctttttc agtgctttct
     3061 gcagattagc atcatgctta taaacaacaa tcatataggc aatttgggca ttcaaacgat
     3121 cataaatggg taaaatgcgc ccgccaccct cattgagaat ttccagctcg gtaccgttgc
     3181 cgaaagtcgt ttcgccggta acaatcagat caccaacgtt ttcaattatg acatccttct
     3241 cgattgtata attgattaaa ttttttacgt tagcgatata gacattatcg gcaatcgtac
     3301 aattgcggat atagctatca taaatgccac aatttttcag acagcccggt ttgacttcct
     3361 gaacaccaca gcaattacct atttctactg ctccggcaaa tgtaacatta tggatattgg
     3421 caggattaaa atcgggctta actgtgatta tttcccagtt ttccgcccga cagccttgtt
     3481 tttcgagaag gttaatttct gcttgagtta attggcgatt ttccattgcc taactttcct
     3541 actaaaccgg caggttattg atagcttcat cctcgatatc tttgaaatat tccacagtgc
     3601 cgactttcaa ttcgagcgtg gcttcatcat cacaaacaat ggtgccgtgc ggatgcaact
     3661 gcaacatcga aaccgtccac atatggttga caccttcttc gatgactttc tgcaaggctc
     3721 gcgctttggt gtatccggag gctatcacca ttacttctcg ggcgtccagc accgttccga
     3781 cacccacggt taaagccgtc ttaggtactc ggctaaggtc accaccgaaa aagcgcgaat
     3841 tagcaacaat cgtatcatag gacagcgtct ttattcgagt ccgtgaagaa attgaagaac
     3901 ccggttcgtt gaaagcaata tgtccattca ccccgattcc tcccagaaaa aaatcaatgc
     3961 cgccgactct tttgattttg gcttcgtatt cttcacattc tttttcaaga tctttggcat
     4021 tcccatccag tatattaata ttttcaggcg gaatatcaat gtgatcaaat aaataatgcc
     4081 gcataaaata atgataactt tccgggtcat cttccgaaag tcctacatac tcatccatat
     4141 taaaagtaat gacattttta aatgatactt taccttcttt atacaatctt accagcgcct
     4201 ggtaagtacc gattggcgaa gagccagttg gtaaaccgag cacaaaaggt ttagcgctag
     4261 tctgattggc atgaataatt ttagccgcaa catatcgggc tacccaatca ctcagtttct
     4321 cataattatt gtgaattatt accctcattt taaattcctt tactaatgaa taattttact
     4381 tttactgaat tactgaatca aaaataccat ttaaagttct tgtagttaca attttatata
     4441 aaattaccaa tcacaaaaat aatatataac aaaaatataa tta
//
LOCUS       DAOMJU010000119         4312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_22301_length_4312_cov_17.951844, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000119 DAOMJU010000000
VERSION     DAOMJU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22301_length_4312_cov_17.951844"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PLO58_08905"
     CDS             <1..1137
                     /locus_tag="PLO58_08905"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQO74945.1"
                     /translation="TFFLQAESQPGTAVLQFSAGEAHTYLTVSFCDTNTTLIAGFCRS
                     AKNSLPIADVLVTLLPDSVQQLTNADGIYYFPHAKPGDYTLNFLKNGYFSQNLEVVVN
                     DGKSNIDTLKMRPIFNGVLHNFVLVLDPRFGGQESGVEISPNLNSAQANLQLAQILKT
                     LFEQAGAEVYLTRDRDSTLSRDQRIKLSNQFPPGGYYLRLNLDCNHQAGRMFKGCHYV
                     GNEEGKRLLQSMANRLTRDGFCDQIQIAGSAEPELVRTNRRALTLDLHLKPQDSLLIS
                     NEASLSRLAWGIFLGFVEELGGENLPKGALTINIRASHKPLADAEVRIGSSLRQLSNQ
                     TGAISYEYLEPGKYYLSISHPIYGKREEIFIIQNSSELLINLSE"
     gene            complement(1250..2284)
                     /locus_tag="PLO58_08910"
     CDS             complement(1250..2284)
                     /locus_tag="PLO58_08910"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO74946.1"
                     /translation="MKNKYFVIGLMILLIVCSHSVFASAPKLGTAGAPELLIPMGAKN
                     VALSGSNIASIAGTDAMYWNPAGMADLKTGEATFTQMSYFADMKVSYLAAGANAGKIG
                     AFGISLQILNIGDIPVTTVNMPEGTGEILSPDYLTLSATYAKRFTDRILFGTNVKLIS
                     EKVGTMSASAVGFDLGLQYISHIGLAFGVTMKNIGSNIEFDGTAIEFDSSVPFANPNA
                     TSRKTKLDMASHELPASMNLGLSYSYKLAELGKINLCGVYSNYSYSYDEMSAGAEFAF
                     RDMLFARAGYLIPLYPSDWEYDETSEYGMSLGFGINLKMAGTNLMFDYAFRPMATFDA
                     NQYFSITIGF"
     gene            complement(2322..>4312)
                     /locus_tag="PLO58_08915"
     CDS             complement(2322..>4312)
                     /locus_tag="PLO58_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74947.1"
                     /translation="DSDGDGMAEFGEPGVQEIVAGVIVARGASNLNSVTTLKEVDNLA
                     QLAYELNFVLAKAPAAPEVTVSELPNEVVLTWSGNSEYNDDGSLYDSPDPIVAQAFGD
                     TVIIDNTEKVITDSTYNFYGYTVYQYSDASGADPVKVESWDIGKNADPMEYTKQRFIR
                     LLVNKHPKVGNVNDPLINGKEYYFGVVAEGYLEFGAPQIFTSSPTIVKVTPQITAGVR
                     YSASYNDTVEVTHTTLNNATPSDGSTIAWVVDPSRVTGADYSVTFNPDLTWNLIKAPN
                     DTVVANNANQTGNDAYNVVDGLMVKVVGPDPGINLSIPGPFGDMPGYNGFGITGGQRW
                     VSWPTNWGMETFAGAFGNGFTFFGSDLGPADYVDVEIRFAGIGPSGWPQPDSSAAALM
                     EVSKTAYPDRWQKAVVYWRPNYEVQLTLGDVPFTVWDMESDPPRQLKVAFVEDPRPAY
                     DGAANLIWDMGWGDDAKFGVNGGREYVFILNDTYDEQYTDYLNGTLDGTYNNVMYAGG
                     WGARSETRPFLQDAFEFQIYASNINTVNDVFSFKAPDAANVEKAYLKEDLKKINVFPN
                     PYYGYHSGEMNIFERWVRFTFLPEKCTIKIFDLSGHLVRELEKNDATTPFMDWDLKNH
                     YGLPVASSVYIYHVEVPGVGEKVGKLAVFTPNERLDTY"
BASE COUNT         1273 a         1016 c          838 g         1185 t
ORIGIN      
        1 actttttttc ttcaggcaga gtctcagccg ggaacggcgg ttttgcaatt ttccgccggt
       61 gaagcgcata catacttgac agtatctttt tgtgatacta ataccaccct gattgccggt
      121 ttttgccgga gcgcaaaaaa tagcctaccg attgcagatg ttttagtcac ccttttgcca
      181 gattcggttc agcagttgac aaacgctgac gggatttatt attttccgca cgccaaaccc
      241 ggagattaca ctttaaactt cctaaaaaat ggctacttca gtcagaattt agaagtagta
      301 gttaatgacg gaaaaagtaa catagatacc ctaaaaatgc gtccgatttt caatggtgtc
      361 ctacataatt tcgttttggt gctcgatccg cgctttgggg ggcaggaatc cggagtagaa
      421 ataagtccaa acttgaattc ggctcaggct aatttgcaat tagctcaaat cctgaaaaca
      481 ctatttgaac aagccggcgc tgaggtttat ctgactcgcg atcgtgactc cactctaagc
      541 cgtgaccagc gtattaaact cagcaaccaa ttcccaccgg gcggctatta cttgaggttg
      601 aatctggatt gcaaccacca agctggcaga atgttcaagg gctgccatta tgtcggcaac
      661 gaagagggaa aacgcctttt gcaatcaatg gcaaatcgtc tgacccgcga tggtttctgt
      721 gaccaaattc aaatcgccgg ttcagctgaa ccggaattgg tcagaaccaa tcggcgggct
      781 ttgacactcg atctgcattt aaaaccgcag gacagcctac taatcagcaa cgaagccagc
      841 ctttcgcggc tcgcctgggg aatttttctg gggtttgtag aagaattagg tggagaaaat
      901 ttaccgaagg gagctttaac aataaatatc agagccagtc ataaaccact cgctgatgct
      961 gaagtcagga tcggttcgtc actaagacaa ttaagcaatc agaccggtgc aatttcctat
     1021 gaataccttg aaccaggtaa atattactta tcaatcagtc atccaattta tggcaaacgc
     1081 gaggaaatct tcattataca aaatagcagt gaattgttaa tcaacttgtc ggaatagatt
     1141 aggaaaatat ttactctcta aaaggatttt cgcactttcg aaaggttcca taataaaaaa
     1201 acggcagtca tttctgactg ccgtttttca tagtttttac cggttattct taaaaaccga
     1261 tagtgataga gaagtattga ttggcatcaa aggttgccat tggccgaaaa gcataatcaa
     1321 acatcaggtt tgtaccagcc atcttcaggt ttataccgaa tccgagagac atcccatatt
     1381 ccgaagtctc atcatactcc caatcgcttg gataaagtgg aattagataa ccagcgcgag
     1441 caaacaacat atccctaaac gcgaactcag ccccagccga catttcatcg taggaataac
     1501 tgtagttact ataaactccg cataaattaa ttttacctaa ttcagctaat ttgtaagaat
     1561 aagaaagacc gagattcata cttgccggaa gttcatgaga cgccatatcc agtttggttt
     1621 tacgggaagt cgcattagga ttagcaaatg gaacacttga gtcaaattct attgcagtac
     1681 catcgaattc gatattactg ccaatattct tcatcgtaac tccgaaagcc aatcctatat
     1741 gagaaatata ttggagtccc aaatcgaaac cgactgctga ggcactcatc gtaccaacct
     1801 tttcgctgat cagttttaca ttagtaccga aaaggattcg atcagtgaaa cgtttagcgt
     1861 aagttgcact caaagtcaaa taatcgggac ttaatatttc cccagtacct tcaggcatat
     1921 taacagttgt gacgggaatg tctccaatat ttagaatttg cagactaatc ccaaaagcgc
     1981 caatctttcc cgcattggct ccagccgcca ggtagctgac cttcatatcg gcgaagtaac
     2041 tcatttgggt aaaagtcgct tcaccggttt ttagatctgc cataccggcg ggattccagt
     2101 acatagcatc cgttccagca atgcttgcaa tattcgatcc gctcaaagca acgttcttgg
     2161 cacccattgg aataagaagc tccggggcac cggcagtacc cagtttagga gcactggcga
     2221 agacgctgtg agaacagacg atgagcaata tcattagccc tattacgaaa tatttgtttt
     2281 tcatattatt tctcctttaa agtccattat tcattcacag cttaataggt gtccagtcgt
     2341 tcgttaggag taaagaccgc taacttaccg actttttcgc caacacctgg tacctcgaca
     2401 tgatagatat aaacactact cgcaaccgga agcccataat ggtttttcaa atcccaatcc
     2461 ataaatggag tagttgcatc gtttttctcc aattcacgta ccaaatgtcc cgacagatcg
     2521 aatatcttaa ttgtacattt ctccggaagg aaggtaaaac gtacccagcg ttcaaagata
     2581 ttcatttcac cgctatgata gccgtaatag ggattcggga agacgttgat tttctttaaa
     2641 tcttccttca gataggcttt ttcaacatta gccgcatccg gagccttaaa agaaaataca
     2701 tcatttacgg tattaatgtt gctagcatag atttgaaatt cgaaagcatc ctgtaagaaa
     2761 ggacgtgtct cagacctcgc tccccatccg ccggcataca ttacattgtt gtatgttcca
     2821 tcaagagtac cattcaaata atccgtatat tgttcgtcat aggtatcatt cagtataaat
     2881 acgtattcac gtccaccatt gacaccaaat ttagcatcat caccccaacc catatcccag
     2941 attaggtttg ccgcaccatc atacgcagga cgagggtcct caacaaaggc tacttttaac
     3001 tgtcgaggtg gatcactttc catatcccaa accgtaaaag gaacatcacc taaggttaat
     3061 tgcacttcat aattaggtct ccaataaaca acagcctttt gccatctgtc tggatatgcg
     3121 gttttactga cttccattaa cgccgcggca ctcgaatccg gctgaggcca gcctgacggt
     3181 ccaatcccgg cgaagcgaat ctcaacatca acatagtctg ccggtccgag atcacttccg
     3241 aagaaggtga atccgtttcc aaaagcgcct gcgaatgtct ccatgcccca gttagtcggc
     3301 cagctaaccc atctttgacc tccagttatc ccaaaaccgt tgtaacccgg catatcacca
     3361 aacgggccag gaatacttaa gttaatccca ggatcgggac caactacttt caccattaaa
     3421 ccatcaacca cattataagc atcatttcca gtctgatttg cattatttgc caccaccgta
     3481 tcattcggtg ctttaatgag gttccaggtt aaatcaggat taaaggtaac agaataatcg
     3541 gctccagtta ctcgagaagg atctaccacc caggcaattg tagaaccatc actgggggta
     3601 gcattgttta gagtagtatg ggttacttcg acagtatcat tataagaagc gctatatctg
     3661 actcccgccg taatttgggg agtaaccttg acaatggtag gcgaactcgt aaaaatctga
     3721 ggcgcaccaa attcaagata gccttctgct acaacaccaa aataatattc ctttccatta
     3781 ataagtgggt cattcacatt gccgactttg ggatgtttgt ttactaatag ccggataaac
     3841 ctctgtttcg tgtattccat cggatcggca ttcttgccaa tatcccagga ttccactttt
     3901 accgggtcag cacctgatgc atcagaatat tgatatacag tataaccata gaaattataa
     3961 gtcgaatcgg ttattacttt ctcagtatta tcaataatga cagtgtcgcc aaatgcttgc
     4021 gctactattg gatccggtga atcgtataaa cttccatcat cattatattc agaatttcca
     4081 ctccaggtta aaacgacttc attgggaagt tcactgacag tcacttccgg cgcagctggt
     4141 gctttagcca aaacaaaatt caattcataa gccaattggg ctaaattatc cacttctttt
     4201 agagttgtta cactattcaa attagaagca ccgcgcgcga caatgactcc agctacgatt
     4261 tcctgcacac cgggttcacc gaattcggcc atgccatcac catcggagtc aa
//
LOCUS       DAOMJU010000120         3824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_26465_length_3824_cov_10.858053, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000120 DAOMJU010000000
VERSION     DAOMJU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26465_length_3824_cov_10.858053"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLO58_08920"
     CDS             complement(<1..716)
                     /locus_tag="PLO58_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74948.1"
                     /translation="MNNNFTSRIAICGTLILILLLAKNIQSAPIDSLAYYQTQYNQQL
                     TKLRTLEKDYDKLLNDINAIDAQIIKINNASNLNWSDKRKLSRLTARKAEINAKMIPI
                     YTELTNQRTILNITFNRYYDFLSKSIDSTVNQYNTVGDSRQRQSLSRTLFELIDQRGQ
                     LLASQRQFSDQNEFPLPEKEDLVALISKYDFNEAIRRDISHILDAKIRQIDLMIAAAN
                     TEKELRQRLNQLNLEMNALSG"
     gene            complement(709..2085)
                     /locus_tag="PLO58_08925"
     CDS             complement(709..2085)
                     /locus_tag="PLO58_08925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQO74949.1"
                     /translation="MNSPKSNYTILIVEDNQSMRDGLLASLQKEGYSVAGVADGQEAW
                     SLLQNQRFDLIITDIKMPGLDGLTLMKKVKNLAPMTNIILITAFPTVDIAVDAMKCGA
                     QDFITKPFALAELRQKVEALYRRKFVQLKPSLSEPEFPDLIGNSPAIRQIRQLIAKIA
                     PGDSPILITGESGVGKELVARLIHQASRQKNGPFLAVNCGALTETLLESELFGHEKGA
                     FTGAHKTHIGKFEQSNSGTLFLDEIGDMSTALQVKLLRVLQTKQFQRVGGEKFIASDF
                     RLICATNKNLEKKIKDGEFRSDLYYRINVIPLTIPPLRERPSDIPLLVEHLLRLKADR
                     LGRKIPRIEPSVMARLISYSWPGNVRELENFIERALVFIEEDIFSEKLFNFGDLPENT
                     SLLNQTDTPAARDLTAQISQIEREMIIKALRDQHGVKQRAAEQLRIKPSTLYYKLDKY
                     GIKPEEYE"
     gene            complement(2089..3534)
                     /locus_tag="PLO58_08930"
     CDS             complement(2089..3534)
                     /locus_tag="PLO58_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQO74950.1"
                     /translation="MIGIKNNDIQSDNPQSGRGFFPPIKLSWKLVLFTVILILFVTAA
                     ITYPIYWQTRQALENQLTNHLEQNIQLVREQIDTSLVNLLADYPELNSVRDSLETKLN
                     WQAGKFSAQAIYLIDASGRLLVIAGNEESALQSATLHAVEIASARNDQVTSSPLFADR
                     TGQYYKSVFTKITENNGQPVVLGLDAGADFLEITGKLRNQILSLTAIILIISIGLVLL
                     LARTMTKPLENLTKYAEAIGQGRSDLPVLNHRRDEIGFLSRTIQTMYGKISQREKENK
                     QFLASVAHEIRNPLGGMKINAELLLEDTDNLTNATKYATAIAREVDRLALIVDSFLAY
                     ARPIETNLIICDLNELLQQVISELQRDFPSAKITVSGQARVKVNPGKIRLCLLNLIRN
                     GLEAVPEAPAIKINITNKPGQTEIRFFNAGMPIAPEIQSQIFEAFFSTKPDGIGLGLA
                     ITKSLVEQHGGQIYLDHSNTSGTEFVLILPE"
BASE COUNT          965 a          806 c          908 g         1145 t
ORIGIN      
        1 ccgctcaggg cattcatttc cagattcaat tgattaagtc tttggcgtaa ttctttctcc
       61 gtgttggcgg cggcgatcat taaatcaatc tgccggattt tcgcgtcaag aatatggcta
      121 atatcccggc gaattgcctc attaaaatcg tatttggaaa ttagtgccac caggtcttcc
      181 ttttccggta acgggaattc attctgatcg ctgaactggc gctggcttgc cagtaattgt
      241 ccgcgctggt caattaattc aaataaggtt ctgctcaaac tttggcgctg gcgcgagtca
      301 ccgacggtat tatattgatt gacagtcgag tctatacttt tcgacagaaa atcataatag
      361 cgattaaagg taatattcag aattgtcctt tgattggtta attcggtata aatcgggatc
      421 atctttgcat tgatttcagc ctttcgggca gttaggcggg aaagtttcct tttgtcgctc
      481 caattgaggt tgctggcatt attgattttg ataatttggg catcaatagc attgatgtcg
      541 ttaaggagtt tgtcataatc cttttcgaga gtgcgtagtt tggttaattg ctggttatat
      601 tgcgtctgat agtaagccag gctgtctatt ggcgctgatt gaatattttt cgccagtagt
      661 aggattagaa tcaacgtccc gcaaattgcg attcttgaag taaaattatt attcatattc
      721 ctcgggctta atgccatact tgtccaattt gtaatacaaa gtgctgggtt tgattcgcaa
      781 ttgttcggcg gcgcgttgct tgacgccgtg ctggtcgcgc agggctttaa tgatcatttc
      841 gcgttcgatt tgactgatct gagcagtcag gtcgcgagcc gctggagtgt cagtttggtt
      901 caataaacta gtattttccg gcaggtcgcc gaaattaaat agtttttccg agaaaatgtc
      961 ttcctcgata aatactaaag cccgttcgat aaagttttcc aattcccgca cattacccgg
     1021 ccatgaataa gatattaagc gagccataac gctgggttcg attcgcggta ttttacgacc
     1081 aagtcggtcg gctttgagcc gaagtaaatg ttcaacgagt aacggaatat ctgaggggcg
     1141 ttcgcgcaga ggtggaattg tcaggggaat gacattgatg cgataataaa ggtctgaacg
     1201 aaactcgccg tctttgatct ttttttcgag gtttttatta gtggcgcaaa ttagtcggaa
     1261 gtcgctggcg atgaatttct caccaccaac gcgctgaaat tgtttggttt gcaatacgcg
     1321 caggagtttg acctgcaagg cggtggacat atctccgatt tcatcgagga acagggtgcc
     1381 gctgttactt tgttcaaatt ttccgatatg agttttatga gcgccggtga aagcgccttt
     1441 ttcgtgacca aagagttcgc tttccaaaag tgtttcagtt aaagctccgc agttgacggc
     1501 gagaaatggt ccgtttttct ggcgactggc ttgatgaatg agccgggcga ccaattcttt
     1561 cccaacgcca ctctcgccgg tgattagaat cggactatcg cccggtgcaa ttttcgcaat
     1621 tagctggcgg atttgtcgga tagccggtga attgccgatc aaatccggga attctggctc
     1681 gcttaggctg ggtttaagtt ggacaaactt acgccgatac agtgcttcta ctttttgtcg
     1741 taattccgcc agcgcaaatg gtttcgtgat aaaatcctgc gcaccgcatt tcattgcatc
     1801 cactgcaata tcaaccgttg gaaaagctgt gatcaggatg atgttagtca tcggcgccag
     1861 gtttttgacc tttttcataa gagtcagacc atcgaggccg ggcattttaa tatcggtaat
     1921 gattagatcg aatcgctggt tctgaagcag actccaggct tcctgtccgt cagccacacc
     1981 tgccacggaa taaccttcct tctgaagtga agccagcagt ccgtcgcgca tagattggtt
     2041 gtcttcgaca atcaagatgg tataattgga tttgggcgaa ttcatatttt actccggtaa
     2101 aatcaagaca aattccgtgc cgctggtatt ggaatgatcc agatagatct ggccgccgtg
     2161 ttgttcgacg agagatttgg tgatggctaa tcccaaaccg atgccatccg gtttcgtgct
     2221 gaagaaagcc tcaaaaatct gagactggat ttcaggtgca atcggcatac cggcattgaa
     2281 aaagcgaatt tcagtctgac ccggcttatt cgtaatattg atttttatgg cgggtgcttc
     2341 cggaacggct tccagcccat tgcgaatcag atttaataga caaagccgga ttttgccggg
     2401 attgactttt acccgcgctt gcccgctgac ggtgatttta gctgacggga aatcgcgttg
     2461 caattcgctg ataacttgtt gtagcaattc gttcaaatca cagataatca gattggtttc
     2521 gattggacgg gcatacgcca gaaaagagtc aacgatcagt gccagtcggt caacttcacg
     2581 agcgatcgca gtggcatatt tagttgcatt agtcagattg tcggtgtctt ccagcagtaa
     2641 ttcagcgttg attttcatac cgcccaatgg gttgcgaatt tcatgggcga ctgaagctaa
     2701 gaactgtttg ttttccttct cacgctgact tattttgcca tacattgttt gtatagttcg
     2761 gctcaagaac ccgatctcat cccgtcggtg gttcagaacc ggaagatcgc tgcgaccctg
     2821 accgattgcc tccgcatatt tagttagatt ttcgagtggt ttagtcatcg tacgcgccag
     2881 aagtaaaacc agaccaatac taattatcaa tataatggca gttaagctta gaatttggtt
     2941 gcgtagctta cctgtaattt ccaggaaatc ggcaccggcg tccagtccaa gcacaaccgg
     3001 ctggccattg ttttccgtaa tttttgtaaa aacagattta taatattgac cagtgcgatc
     3061 ggcaaacagg ggcgaagagg tcacttgatc gtttcgagcg ctggcaattt cgactgcgtg
     3121 cagggtggct gattgtaatg cgctttcctc atttccagcg attaccagta agcgaccgct
     3181 ggcgtctatc aggtaaatgg cttgagctga aaatttgcca gcttgccagt taagtttagt
     3241 ctcgagtgaa tcccgcacgg aatttaactc cgggtaatcg gcgagcagat ttacaaggga
     3301 agtatcaatt tgctcccgga ctaactggat attttgttcc agatgattgg tcaactggtt
     3361 ttcgagcgcc tgccgcgttt gccagtaaat cggataagtg atagccgcgg tgacgaacag
     3421 gatcaaaatg acggtgaaaa gcaccagctt ccacgagagt ttaatcggcg ggaaaaatcc
     3481 tctgcccgat tgtggattat cagattgaat gtcattattt tttatcccta tcattagtca
     3541 gagctaagtt aattgattta tttcaggacc aaatttcttc ttatttccaa ataccgctca
     3601 aactgagcga gacttcgtga atcggttatg aaatacataa acatagctaa atttagaaca
     3661 tttagcgaat taccaaatcg gaaatataaa accggcaagt aactcgcctt aaaaatcaat
     3721 ttctgctcgt aagcggctgg tcttttccta cagcaaataa atttttacta aaaattactt
     3781 gactttggga gtctaattag ctttaattct ggaaaattca gagg
//
LOCUS       DAOMJU010000121         3778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_26963_length_3778_cov_20.239860, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000121 DAOMJU010000000
VERSION     DAOMJU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26963_length_3778_cov_20.239860"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PLO58_08935"
     CDS             complement(<1..864)
                     /locus_tag="PLO58_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQO74951.1"
                     /translation="MLQLKQVKKVFNKTVAVDSLDLNIPTGSIFGLLGPNGAGKTTTI
                     RMIMNIIQPDAGEILYNGKHLERSDLLKVGYLPEERGLYQKMKLKETVVYLARLKGLS
                     LAEANQCVDEYLRRFDLSGYSHRRIQELSKGNQQKVQFIVSVIHKPELLILDEPFAGL
                     DPVNQLLLKEIIAELQGRGATVIFSTHQMEQVEKLCDRICLINKGKPILNGDLRQIKK
                     EYGQPMLLIDYDGKADTLADLNLPDMQITDNRLTCRLTGNLNELLLSVMQRVQVTRFE
                     LQEPSLEQIFIE"
     gene            complement(897..2114)
                     /gene="tyrS"
                     /locus_tag="PLO58_08940"
     CDS             complement(897..2114)
                     /gene="tyrS"
                     /locus_tag="PLO58_08940"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQO74952.1"
                     /translation="MFPPVKEQLEIIKRGVEELISEEEMVRKLENSIKTNQPLRIKQG
                     FDPTAPDIHLGHTVGIRKLRQFQELGHTIVLIIGDYTGLVGDPSGKNETRPRLQYETL
                     MKNAETYENQFFKILDRSKTEIHYNGEWFAKMKFDEIMNLAAKFTVARILERDDFTKR
                     YKEGNPISLHEFFYPLMQGYDSVAIKADVELGATEQKFNLLTARDVQREYGQEPQVIL
                     TLPVLEGIDGGQRMSKSLGNYIGIDDEPDEMYGKIMSIPDNLILKYFTLVTDYSLAQL
                     QEVKRRLNDGQTNPMVLKKELARAIIKMYHSESAARQAEQNFEQIFSKKETPDDIEEI
                     IIPSSDLPKPLVKLLTECGAVSSNGEARRLIQQGGVRINDEKISDINYAIQNKGEYIT
                     KVGKRRFFKFIVK"
     gene            complement(2117..2578)
                     /gene="smpB"
                     /locus_tag="PLO58_08945"
     CDS             complement(2117..2578)
                     /gene="smpB"
                     /locus_tag="PLO58_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQO74953.1"
                     /translation="MPKDDVKIITTNKKAFHDYFILDKIEAGIELKGTEVKSVRAGHI
                     NLKDSYAKIIDRELYLLKCHIAPYDFGGYDNHNPERDRRLLLHRNEIRRLQREVENKG
                     VTLIPLRVFITDKGKVKVEIGLAKGKRQYDKRNALAEKEARREVERLRKGK"
     gene            2665..2748
                     /locus_tag="PLO58_08950"
     tRNA            2665..2748
                     /locus_tag="PLO58_08950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2699..2701,aa:Leu,seq:cag)
     gene            complement(2843..3229)
                     /locus_tag="PLO58_08955"
     CDS             complement(2843..3229)
                     /locus_tag="PLO58_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74954.1"
                     /translation="MRRRNLGILVISLMILFGIGVLFVGQEYSSSSKLMTVRVEGLPD
                     AQSGKNMQDLMKMTEGIESFIFDQNVGFCTFRYDSSIINYKAIEAQFLALGLKITPIA
                     PIEITSIISLPPEQKLLKVHFFQPTE"
     gene            complement(3592..>3778)
                     /locus_tag="PLO58_08960"
     CDS             complement(3592..>3778)
                     /locus_tag="PLO58_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HQO74955.1"
                     /translation="RKRIETLFSQLVDQFVIRINYTKIFVGLATRILSKVTAFTLLQF
                     LNSINNKPLNHIKHALA"
BASE COUNT         1001 a          817 c          771 g         1189 t
ORIGIN      
        1 ctcgataaag atttgctcca gagatggctc ctgcaattca aaacgagtta cctgcacacg
       61 ttgcattact gagagtaata attcattgag attaccggta agacgacagg taagtcggtt
      121 gtcagtgatt tgcatatcag gcagattaag atcagccaat gtatccgctt tgccatcata
      181 atcaatcaaa agcattggtt gaccatattc tttcttaatc tgccgcaagt cgccgtttag
      241 aattggttta cctttattta tcaagcaaat ccggtcgcat agcttctcga cttgttccat
      301 ttggtgagtc gaaaatatta cggtcgcgcc gcgtccctgc aattcagcaa taatttcctt
      361 caatagcagc tgatttaccg ggtcgagacc agcaaaaggt tcgtccagga taagaagttc
      421 cggtttgtga attaccgaaa caatgaattg gactttctgt tgattgcctt tggaaagttc
      481 ttgaatgcgg cgatggctat aaccagagag atcgaaacgc cgcaggtatt cgtccacgca
      541 ttggttagct tctgccagag atagaccttt gaggcgggca agataaacga cggtttcctt
      601 caatttcatt ttctggtaca atccgcgctc ttccggcaaa taaccaactt tcaataaatc
      661 agaccgctcc aggtgttttc cattataaag aatttctccg gcatcaggct gaatgatatt
      721 cattatcatc cgaatagtgg tagttttccc ggcaccattt ggccctaata aaccgaaaat
      781 cgagccagtt ggtatattaa gatcgagact atcaaccgcc accgttttat tgaatacttt
      841 cttaacctgt tttaattgta acattcagca atcttcgatt tattgaattc aggtgattat
      901 ttaacgatga atttgaaaaa ccgccgtttc ccgactttgg ttatatattc gcctttattt
      961 tgaattgcat aattgatatc gctaatcttt tcgtcgttga tgcgtacgcc gccctgctga
     1021 attaggcggc gagcttcacc gttagaagaa accgctccgc actcggtaag taatttgact
     1081 aaaggttttg gtaaatccga tgagggaatt attatttcct cgatgtcgtc gggggtttct
     1141 ttcttgctga aaatttgttc gaaattttgt tcagcctgac gggcggcgga ctctgaatga
     1201 tacattttga taattgcgcg ggctaattcc ttttttagca ccattggatt ggtctgcccg
     1261 tcatttaggc ggcgctttac ttcctgaagt tgggcaagcg aataatccgt caccagagta
     1321 aaatatttaa gaatcagatt atcgggaatg gacataattt tgccgtacat ctcgtccggt
     1381 tcatcgtcaa tgccgatata attaccgaga cttttgctca ttcgttgacc accatctatg
     1441 ccttccagca ccggcagagt taatataacc tgtggttcct gaccgtattc gcgttgaacg
     1501 tcacgcgcgg taagcagatt gaatttctgt tcggtggcgc cgagttcgac atcggctttg
     1561 attgccacgg aatcgtagcc ttgcatcaat ggatagaaga attcgtgcag actaatcgga
     1621 ttgccttctt tataacgttt ggtaaaatcg tctctttcga ggatccgtgc aacggtaaat
     1681 ttcgccgcca gattcataat ttcatcgaat ttcattttgg cgaaccattc accgttatag
     1741 tggatttcgg ttttacttct atccagtatt ttaaaaaatt gattctcgta agtttcggca
     1801 tttttcatca aggtttcata ttgcaatctt ggtcgcgttt cgttcttgcc actcggatca
     1861 ccaaccaagc cggtgtaatc gccgataata aggacgattg tatgtcccag ttcttggaac
     1921 tgacgcaatt tccggattcc gaccgtatgc ccaagatgaa tatccggtgc ggttggatcg
     1981 aagccctgct tgattctgag cggctgattg gttttaattg agttctcgag ctttctgacc
     2041 atttcctctt cggaaattag ttcctcgact cctcgtttga tgatttccag ttgttcttta
     2101 accggtggga acatgcctat tttccttttc ttaaacgttc aacttcgcgg cgcgcttctt
     2161 tctcagctag cgcgttcctc ttatcgtact gtcttttgcc tttggctaaa cctatttcga
     2221 ctttaacttt ccccttatcc gtaataaaaa cgcggagcgg aatcagggtt acgcctttat
     2281 tctcgacttc gcgttgcagg cgccgaattt cattccggtg caatagcaga cggcgatcgc
     2341 gttccggatt atgattgtcg tatccgccaa aatcgtaggg agcaatatga catttcaata
     2401 aatataattc ccgatcaatg attttggcgt aactgtcttt gagattgatg tgcccggctc
     2461 tgaccgactt gacttcggtg cctttgagtt cgatgccggc ttcaatttta tcaagaataa
     2521 aataatcgtg gaaggctttc ttattagtcg ttataatttt gacatcatct tttggcatac
     2581 ggaaaattta attcttttgc ctcaacttcc aatgtaaaac ttggagaact cagtcggcgg
     2641 ttataaattt tgccgttatt tgaagcccgg gtggtggaac tggtagacgc gctaggttca
     2701 ggacctagtc tccattagga ggtgcgagtt caagtctcgc cccgggcaca aagattgaat
     2761 aatgcgaaat taggcttaat tacttatatc ggtaatcaac gctgttcagt ttggaattat
     2821 ctcgctaact cggcaatgtc tgtcattcgg tcggctgaaa aaagtgaact ttcagcaatt
     2881 tttgttccgg aggcagagag ataatactgg ttatttcgat tggagcaata ggcgttattt
     2941 tcagaccaag cgccaaaaac tgagcttcga tagctttgta atttataatc gaagaatcgt
     3001 aacggaaagt gcaaaatccg acgttttgat cgaaaatgaa cgattcgatg ccctccgtca
     3061 ttttcattaa atcctgcatg tttttacccg actgcgcatc cggtaaacct tcgacccgaa
     3121 ctgtcattaa tttactgctg gatgaatact cctgcccaac gaaaagcaca ccgataccaa
     3181 acaggatcat caacgaaatc accaaaatgc ccaaattgcg tcgcctcata cagatgcttc
     3241 cttttagcta ctaccccaaa aaaagcgata taatttaagg acttactaac taaaatgaaa
     3301 gtaatataac tagccaagga agcccagaat tttttatttt ttatccacaa cctttttgcc
     3361 cactgggacg tctgctcatg gttattcaac ctttccgcta tacatggaat ttttatctca
     3421 ttgagcacat gtaaagtatt cccgcttaat cgaagaacta caatcaccta acttctattt
     3481 ataaacataa tattgaagag ttacttcttc catctcattg ctaggaatct gtatcagttg
     3541 atacatcaat tcttttagtg aagactcata agtattaccc gttgtgtgga attaagcgag
     3601 ggcatgttta atatggttta agggtttatt attgatagaa ttcaggaact gtagcaaagt
     3661 gaaggcagtc actttcgaca aaatcctggt agccaagcca acaaagattt tggtataatt
     3721 tatacgaatc acgaattgat ctaccaactg cgaaaacaag gtttcaatcc gtttacga
//
LOCUS       DAOMJU010000122         3753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_27226_length_3753_cov_12.804759, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000122 DAOMJU010000000
VERSION     DAOMJU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27226_length_3753_cov_12.804759"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLO58_08965"
     CDS             complement(<1..235)
                     /locus_tag="PLO58_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74956.1"
                     /translation="MNKINKLEIRNMKPLSGEIDLCFRPPELVTEINPCLIKYKGAPK
                     PEQALMAKSANGIAVVTGCSHPGIITILTKVKTI"
     gene            685..1893
                     /locus_tag="PLO58_08970"
     CDS             685..1893
                     /locus_tag="PLO58_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO74957.1"
                     /translation="MRIFRVVLILSTVFVISSAQWVQMNAPSSSGITYSSFVSTEGAF
                     LVGSNGLGIHQSIDEGLNWTKIANATIENSTHIMTAIDTNIYLGMNTTGVAFSSDNGA
                     TWTNISNGITNKKIMTIAKMGRYLFAAGTSFYRSSDLGATWTESGAGLPTSDKKDIII
                     MDNKVFICAKGSGVYLSTDSGATFTSHNGNLPHTYTKRLLQKGEKVFLWLGNAPYVST
                     DSCATWTTASTGSVDAEPLCVYTDGNIIFNGTFGSGIYYTNNDGGQWLSDNTGLTNLN
                     VRGIIVRGGYVFVGTDGAIWRRPLCEFTTAISKDRRTSTGNFSLFPAYPNPFNPSVTI
                     PYSLAEDGQMSLKVFNLRGELVEVLISNYTLKGTYSVTWQPQNISAGIYLVRLESGNK
                     TNLQKVVFVK"
     gene            2349..>3753
                     /locus_tag="PLO58_08975"
     CDS             2349..>3753
                     /locus_tag="PLO58_08975"
                     /inference="COORDINATES: protein motif:HMM:NF033578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HQO74958.1"
                     /translation="MVNYEDPNQTKIVKFTLPFSGHLDPNNRWIRLAEMVPWQELYGK
                     YTKQLCRDFGRMSIKPRVAIGALIIKHFKQLSDEETIQEIQENPYLQYFLGYSSYQFK
                     PGFDPSLFVTIRRRLPARTIEAINEIFIQKVREYEQIEAEHKQSRRQSKACKKGGKTS
                     FPLENSDSSSNKGKLIVDATIAPSNIKYPTDLDLLNDSREISEKLIDKLYLKGLFEHK
                     PRTYRQKARQDFLNVIKKKRKSKKALCKGIRKQLQYLRRNLTHLDQMLAVFKDQPDAL
                     SEREIELLEVIRKIYAQQKEMYINQRHTVKDRIVSIHQPYIRPMVRGKANAEVEFGAK
                     ISVSVVAGYVFLDRLKWDAYNENMDLVDQVNRYRQRFGYYPAVVIADGIYGTRDNRSY
                     LEEHGIRFSGKKLGRPPKEISAIAWEMERLRILEQGERNEVEGKIGTAKTRYGLGKVL
                     ARTKETSENWIAMALLSM"
BASE COUNT         1115 a          728 c          873 g         1037 t
ORIGIN      
        1 aaattgtctt cactttcgta agaattgtaa tgattcccgg atgagaacag ccggtgacta
       61 ccgcgatccc attcgcggat ttagccatca aggcttgttc cggtttcggc gctcccttat
      121 attttattag gcagggatta atctccgtga caagttctgg tggcctaaaa cacagatcaa
      181 tttctccact caatggtttc atattcctaa tttctaactt atttatttta ttcacaacaa
      241 aacgtacaag aacacggagg atatttccta cttgtagggg tacgcggcgc gcccttgtat
      301 ttctgaataa gaggaatagt tcaggtctcg tcgccgtgta catccccgga gtgacaagag
      361 aaaaacacgt aacctaagcg attcttcagt tttgtcagat gtcgaatcct gacggacttt
      421 taacagatca ctgccaacca tttgaatggt taaattgaaa atattattgc acatcaggaa
      481 aattaattat aaatttaatc cgtatagttt tagtggattt ttacattaat ctgaaccaag
      541 tgaggtcatg tgagaagatt atctgcaatt ctcagaatgg taaatattta tattttcgat
      601 tataaaattg atagataaat tgatctgttg cttggtagat aagcaccaag tcagagaaga
      661 cttaatataa aaaggagaga gcgtatgaga atttttcgtg ttgttttaat tttaagcacg
      721 gtttttgtga ttagttcggc acaatgggta cagatgaacg caccgagttc aagtggaata
      781 acctatagtt cctttgtgtc gacagagggt gcattcctgg tcggttcaaa tggtttaggt
      841 attcatcaaa gtatcgatga aggattgaat tggacaaaga tagccaatgc aaccatcgaa
      901 aactcaaccc acatcatgac agcgatcgac acaaatatct acctgggtat gaataccact
      961 ggtgttgcct tctcttcaga caacggtgcg acctggacaa atatttcgaa tggaataacc
     1021 aataaaaaaa tcatgacaat cgcgaaaatg ggccgctact tgttcgcagc cgggacctcc
     1081 ttctatcgct caagcgatct aggcgcgacc tggactgaat ccggggcggg actcccgact
     1141 tccgataaaa aagacataat cattatggat aataaggtgt ttatttgcgc aaaaggctcc
     1201 ggtgtttacc tttccaccga ctcgggagcc acctttacat cacataacgg aaaccttcct
     1261 cacacttata ccaaaagact cttacagaaa ggggaaaagg tgtttttatg gcttggtaat
     1321 gctccatacg tcagtaccga ttcttgcgcc acctggacga ctgcctcgac aggttccgtc
     1381 gatgctgaac cattatgcgt ctacaccgat ggcaacatca tatttaacgg cacctttggt
     1441 agcggtattt attatactaa taacgacggc gggcagtggc tatcggataa caccggtttg
     1501 actaatctga atgtacgcgg aataattgtc cgtggtggat acgtttttgt tggtaccgat
     1561 ggagcaatct ggcggcgacc gttatgcgaa tttacgacgg cgattagcaa agaccgcagg
     1621 acttcgacgg gtaatttctc attatttccg gcctatccga atccgttcaa tccgagcgtg
     1681 acaattccat acagtctggc agaagacggc cagatgtcgc tgaaggtttt taatctgcgc
     1741 ggtgaattag tagaagtact tatcagcaat tatactctta aaggcactta ctcggtcacc
     1801 tggcagccgc agaatatcag cgccgggatt tacctggtac gcctggagtc cggtaacaaa
     1861 accaacctgc agaaagtggt cttcgtcaaa taattgcaac ccgtctgccc gactgctgac
     1921 gcagtcatac aggcggagag ttccacggag gatattttcg gggaacacgg agaatattat
     1981 ataggggtcc ggcagctgcc ggactcttct tatttatact ggcatgtttc tgttggggca
     2041 cacagtcccg acacatcgga atgccctttt tcataccaat cattttgatc tcctggttac
     2101 accgctccag cggcgtaact aattatgatg gatttaacgg cccacacttt ttattttgat
     2161 acgagtcttt aataagattt tgactgaagc cacgtttacg tcaaaataat aattagggct
     2221 tcctgaaaaa gtatctaata tattgtaata acagatattt atcgcttgct ttatttgatg
     2281 taaatgtata tatttaggat gatatttggc aaaacctatg catttaacaa atcaatttgg
     2341 agctctaaat ggtaaattat gaagacccca atcaaactaa gattgtaaaa ttcaccttgc
     2401 ctttttctgg tcaccttgat cctaacaatc gctggatacg tttagctgag atggtaccgt
     2461 ggcaggaact ctatgggaaa tacactaagc aactgtgtcg agattttggg cgtatgtcca
     2521 taaagccacg agtagccata ggtgcattga ttattaagca cttcaagcaa ttaagtgatg
     2581 aggagacgat ccaggagatt caggagaatc cttatttgca atacttttta ggctattcga
     2641 gttatcaatt caagcccggc tttgatcctt ccttatttgt gactattcgg aggcgcttgc
     2701 cagcccggac gattgaagcc atcaatgaga tattcattca gaaagtgaga gaatacgagc
     2761 aaatagaagc cgagcataag cagagtcgga gacagtccaa ggcttgcaag aaagggggaa
     2821 aaacgtcttt tccgctggag aatagtgatt caagtagcaa taaaggcaaa ttgatagtag
     2881 atgctacgat tgcaccctct aatatcaaat acccgactga tttggattta ctcaatgact
     2941 cgcgagagat cagtgaaaaa ttgattgata aactgtattt gaagggatta tttgagcata
     3001 agccgaggac ttatcgtcag aaagcgcgcc aggattttct gaatgtaata aagaagaagc
     3061 gcaaaagtaa gaaagcgctt tgtaagggaa tccggaagca gttgcaatat ttgaggcgga
     3121 atttaactca tcttgatcag atgttggcgg tatttaaaga tcaacctgat gctcttagtg
     3181 agcgtgagat cgaattactg gaggttattc gtaagattta tgctcagcag aaggagatgt
     3241 atattaatca gcgacatact gtaaaggatc ggattgtgag tattcatcag ccttatatac
     3301 gaccgatggt cagggggaaa gccaatgcgg aggttgagtt tggagctaaa atatcagtaa
     3361 gtgttgtggc ggggtatgta tttttagatc gattgaagtg ggatgcctac aacgagaaca
     3421 tggacttggt tgatcaggtg aatagataca ggcaaaggtt tggctattat ccggcagttg
     3481 tgattgcaga tggcatatac ggaacccggg ataatcgcag ttatctggag gaacatggca
     3541 ttcgattctc tggtaagaaa ttgggtcgtc cgcctaagga gataagtgcg atagcctggg
     3601 aaatggaacg attgagaata ctggagcaag gtgaaaggaa tgaggtcgaa gggaaaatag
     3661 gaacagccaa gacccgctat ggattaggaa aagttctggc caggactaaa gaaaccagtg
     3721 aaaactggat agccatggcg ctactctcga tga
//
LOCUS       DAOMJU010000123         3557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_29411_length_3557_cov_23.170188, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000123 DAOMJU010000000
VERSION     DAOMJU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29411_length_3557_cov_23.170188"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /locus_tag="PLO58_08980"
     CDS             <1..1686
                     /locus_tag="PLO58_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQO74959.1"
                     /translation="EMERLGLLEKIEEYSHSVGYSERAGVMIEPYLSEQWFVRMQPLA
                     EPALKVVEEKEIRFYPEKWVKVYKHWMTNIRDWCISRQLWWGHRIPVFYCDACGWSDA
                     LMEDPSACPKCGGAVRQDEDVLDTWFSSWLWPFSTLGWPDDTPDLRKFYPTDDLVTGP
                     DIIFFWVARMIMAGLEFCHEIPFRNVYFTGIIRDAQRRKMSKSLGNSPDPLVLVEKYG
                     ADALRFGIMLIAPQGQDILFSEERLEVGRNFMNKVWNAARFVLMNLDENTLSGEPFEP
                     KRMQLELADEWILSRLNDTIRSVDKNLKKYRFDTVARTIYDFVWSDYCDWYIELIKDR
                     LYKKSAAEKQTALQVAVYVLKNFLKLLHPYAPFITEEIYQHIKLPNEPDIVVSTWPKV
                     EYHPKDKALTERFELLQEIVTALRTARSEMNISPAVSIELIVRSASNSPVTELLNNSE
                     IQNYIKNLVKVTQITFVAENVKPAHSSTVVVRGTEFFIPLEGVIDIAAETARLTKEIN
                     RLTGILKGASAKLSNRDFLARAPQEIINKERAKEQVLKEQLDKFKANLKLLNN"
     gene            1732..3111
                     /locus_tag="PLO58_08985"
     CDS             1732..3111
                     /locus_tag="PLO58_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74960.1"
                     /translation="MKKLKNGILLATIILGLIRMIFADTPVKSTQITVYNNNLGLVLQ
                     TRTLNIAKGDSEIRIEGVPAKVDPTSVRITFPGKADAIEVLEQNFLYDLVNSIKIFEK
                     YIGESISYKTKSGEQTIGKLLQFDGGNLILQLPNGGIKIASAENISEYDFPSLPSGLI
                     LKPTLQWLLSSRINAAVESEISYLTAGMNWHAEYLMVLDKNDRDFTLNSWVSLDNQSG
                     ATYENAKMKLVAGDIHRAPQPAPVRREKVYAADVLTQVAPFEERGLFEYHLYELQRPV
                     TIKDREIKQITLFDEVNAKAEKFYLFKNSAFSETEGPLEVHLKFANSTANHLGIPLPE
                     GVFRIFKKDVDNTLQLVGEDRIAHTAKDDTLDLIVGKAFDVKGQKTITDRQKISDRSE
                     IISIKLEISNKRTEPINVEIQEIMSGDWFVKNASHPYQKKSNSLLVFPLTVPAHKNIQ
                     IKYSFQRSW"
     gene            3105..>3557
                     /locus_tag="PLO58_08990"
     CDS             3105..>3557
                     /locus_tag="PLO58_08990"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQO74961.1"
                     /translation="MVNERPLDKRNIELRPETPITYVKGVGPKRAEVLAKVGINTLKD
                     LLYYFPRRYLDRSTITEIAQLKVGMDATVVGKIISQNLVRRYRRPFFQVEISDGSGIM
                     TAVWFNGINYIKNAFKVGETVAMSGKVEFFKGLRFIHPDYDKLETDEWE"
BASE COUNT         1001 a          839 c          819 g          898 t
ORIGIN      
        1 gaaatggaac gcctcggttt gctggaaaaa atcgaggagt actcgcattc cgtcggatac
       61 agcgagcggg ctggagttat gatcgagcct tatctttccg agcagtggtt tgtgcggatg
      121 caaccgctgg cagaaccggc gctaaaggtc gtcgaggaaa aggaaattcg tttttatcct
      181 gaaaaatggg tcaaggttta taaacattgg atgaccaata tccgcgactg gtgcatctca
      241 cgccaactct ggtggggtca tcgcattcca gttttttact gcgacgcttg tggctggagc
      301 gatgctctga tggaagaccc gtccgcttgt cctaaatgcg gcggcgccgt gcgtcaagat
      361 gaggatgtac tcgacacttg gttcagttcc tggttgtggc cattctcgac gttaggctgg
      421 ccggatgata cccccgattt gcgcaaattt tacccaaccg atgatctcgt cactggtccg
      481 gacatcattt tcttctgggt agcgcgaatg ataatggctg gactcgagtt ctgtcatgaa
      541 attccttttc gcaatgtcta tttcaccggc ataattcgcg acgctcagcg ccgcaaaatg
      601 agtaaatcgc tcggcaattc tcccgatccg ctggttctag tcgagaaata tggcgcggac
      661 gctctgcgtt ttggcattat gctaattgcc ccgcaaggtc aagatatttt attcagtgag
      721 gaacggctgg aagtcggacg caattttatg aataaggtct ggaacgccgc ccgctttgtc
      781 ttgatgaatc tggatgaaaa tacgctaagc ggtgaacctt tcgagccgaa gcgtatgcaa
      841 ctggaattgg cagatgagtg gattctaagc cgcttgaatg atactattcg ctccgttgac
      901 aaaaatctta aaaagtaccg tttcgatacc gttgcgcgca cgatctatga tttcgtctgg
      961 agcgactact gcgactggta tattgaactc atcaaggatc ggttatacaa aaaatcagcg
     1021 gcagaaaaac aaactgcctt gcaagtggcg gtctatgttc taaaaaattt cctcaaactg
     1081 cttcacccct atgcaccctt cattactgag gaaatctacc agcatatcaa attgcctaac
     1141 gagccggaca tcgtcgtctc aacttggcca aaagtcgaat atcaccccaa ggataaagcc
     1201 ctgactgagc gtttcgaact cctgcaggaa atcgtcaccg ctctgcgcac cgcccgcagt
     1261 gaaatgaaca tttcaccggc tgtgtcgatc gaattgatag ttcggtctgc gagtaattcg
     1321 ccggtcaccg aactactgaa caattccgaa attcaaaact acattaaaaa tctggttaaa
     1381 gttactcaaa taacgttcgt tgccgaaaac gtcaaaccgg ctcattcttc aacggtcgtt
     1441 gtccgcggca ccgagttttt cattccattg gaaggtgtaa ttgatattgc cgccgaaact
     1501 gcgcgcctca cgaaggaaat caaccgttta accggaatat taaaaggcgc ttctgccaaa
     1561 ttatccaacc gcgacttcct cgcccgtgcg ccgcaggaaa ttataaacaa agaacgcgca
     1621 aaagagcaag ttctgaagga acaattagat aaattcaaag cgaaccttaa gttgctgaat
     1681 aactaagtca cgttcgctga ttatttttta aatcaaattg aggagagtcg tatgaaaaag
     1741 ttaaaaaatg gaatactttt agcgacgatc attttgggac ttattaggat gattttcgct
     1801 gatacgcccg tcaagtccac tcaaattacc gtttataata acaaccttgg tttggtactc
     1861 cagactcgca cgctcaatat cgccaaaggc gattcggaaa ttcgcattga aggcgttccg
     1921 gctaaagttg atccgacctc ggtgcgcatt acatttcctg ggaaagccga cgctatcgaa
     1981 gtcctcgagc agaattttct ctacgatctg gtcaattcta tcaaaatatt cgaaaaatat
     2041 atcggcgagt caatctcgta taagaccaaa tctggtgaac agactattgg aaaactgctc
     2101 cagttcgatg gaggtaatct cattttgcaa cttccgaatg gtggcatcaa gatcgcttcg
     2161 gctgaaaata tttccgagta tgacttccca tccctgccgt ccggattgat tttaaaaccg
     2221 actttacaat ggctgttgtc gtcgagaatc aacgccgcag tcgaatccga aatcagctat
     2281 ttgactgccg gaatgaactg gcacgccgaa tatttaatgg tgctggataa aaatgaccgg
     2341 gactttacac tcaattcctg ggtgtcgctc gataatcaat ccggcgccac ttacgaaaac
     2401 gccaagatga agctggtcgc cggagatatt catcgcgctc cccaaccggc gccggttcgc
     2461 agagagaaag tctatgccgc cgatgtcctt acgcaagtcg caccctttga agaacgcggc
     2521 ttgttcgagt accatctgta tgagctccag cgtcccgtaa ccattaaaga tcgggaaata
     2581 aaacaaatta ccttattcga cgaagttaac gccaaagcgg agaaatttta tctattcaaa
     2641 aattccgctt ttagcgaaac ggagggaccg ctggaagtcc atttgaagtt tgccaattca
     2701 accgccaatc atctcggtat tccgctaccg gagggcgttt tccgtatttt caaaaaggat
     2761 gttgataata ccctgcaatt agtcggcgaa gatcggattg cgcacaccgc caaagacgat
     2821 accctcgact tgatagtcgg caaggctttc gacgtcaaag gccaaaaaac aatcaccgat
     2881 cgccagaaaa tcagcgaccg ttcggaaata atttcgataa aattggaaat ttcgaataag
     2941 cgcacggagc cgatcaacgt cgagattcag gaaataatga gcggcgattg gtttgtgaaa
     3001 aatgcgtcgc atccttacca gaagaaaagc aatagtctgc tggtttttcc attgactgtc
     3061 cccgcccata aaaatatcca aatcaaatat tccttccaga gaagttggta aacgaacgac
     3121 cgctcgataa aagaaacata gaactgcggc cagaaacgcc gattacctat gtcaaaggcg
     3181 tcggtccgaa acgcgccgaa gtgctggcga aggtcggcat taacacttta aaagatttac
     3241 tatattattt tccacgccgc tatctcgatc gtagtacgat tacggaaatc gctcagttaa
     3301 aagtcggtat ggatgccacc gtcgtaggaa aaataatttc gcagaatttg gttcggcgct
     3361 atcgccgacc gtttttccag gtcgagatta gcgacggtag cggcattatg acagcggtct
     3421 ggttcaacgg tattaattac atcaaaaatg ctttcaaggt cggcgaaaca gtcgcaatga
     3481 gcggcaaggt ggaatttttc aaaggcttgc gcttcatcca tcccgattat gataaactcg
     3541 aaaccgacga atgggaa
//
LOCUS       DAOMJU010000124         3544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_29571_length_3544_cov_13.012898, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000124 DAOMJU010000000
VERSION     DAOMJU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29571_length_3544_cov_13.012898"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="PLO58_08995"
     CDS             <1..2085
                     /locus_tag="PLO58_08995"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HQO74962.1"
                     /translation="GSSFTRANHIWVGWPGTVVEDKSKKKLSTKLQDDFDAYPVFLAE
                     ETMEKFYHGFCNKTIWPLFHYFTSYVEYDEECWEHYQRVNEAFRDAVMEIIKPDDIIW
                     IHDYHLMLLPKILRLKISNPIGFFLHIPFPTFEIFRLLPGTWRREILQGLLGADLIGF
                     HSYDYTQYFLRCVLRILGYEHNMGQIIINDRIIKADTFPMGIDFQKFYNAVNSPEVQK
                     EKNEFQKTLGNFKIILSIDRLDYTKGILNRLRGYEAFLDKNPQWHRKVILVLIAVPSR
                     IGIEHYREMKRQIDELVGKINGRFGSINWTPILYQFKFVPFNQLVALYNISDVALITP
                     LRDGMNLIAKEYISAKADSAGVLILSEMAGAAKELGEAIIINPNNLPEITDALKIALE
                     MPKEEQLKRNQVMQKRLKRYDVVKWADDFIQDLLSLKENQKNFNAKLLSSAIRNRLIK
                     DFSQANRRLIILDYDGTLVPFVDQPQMASPDEQLLEILQSLAENPKNEIVLISGRDKD
                     TLQNWFGKLDIGLVAEHGVWLKEKNKDWDIIKPLTNDWKSKIIPLLEVYTDRLPGSFI
                     EEKEFSVAWHYRMAEPELGSLRAKELTDYLLNFIANIDIQVQQGKKVVEITNAGINKG
                     AAGLQWIGKKDFDFILAIGDDWTDENLFEVLPERAYSIKVGMVETHARFSLYNHTEVI
                     ELLKNLLKVNGN"
     gene            2173..3381
                     /locus_tag="PLO58_09000"
     CDS             2173..3381
                     /locus_tag="PLO58_09000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO74963.1"
                     /translation="MAKLIDYRGIVGDKVITGIFQKARGLYGKKIIHLNSTYMGGGVA
                     EILNSLVPLMNDVGLDTGWRIIRGNPDLFTITKKFHNALQGDPINLSDMKKEIYIKAS
                     EDFSVFTHLDHDCVIIHDPQPLPLIRYYVKQQPWIWRCHVDISHPNPVLWDFLKAFIL
                     KYDLAIVSHETYKKDDIYIEQKVIPPVIDPLSPKNKELSEKDTKRFLKKFGIPIDKPL
                     LTQISRFDKWKDPEGVVRIFKKVKKQIDCRLVLCGSMATDDPEGMKIYQKVVDYSNHL
                     IQSGDIIPITSENNILVNSLQRYSDVVIQKSIREGFGLVVAEALWKKAAVVASNVGGI
                     PLQVIDGETGFLCEPNDEEAFAERIIQLMKDKKLKEKLGQNGKEHIKSNFLITRLLCD
                     YLDVIRSLLS"
BASE COUNT         1145 a          666 c          723 g         1010 t
ORIGIN      
        1 ggctcatctt ttaccagagc aaatcatatc tgggtgggct ggcctggaac ggtagttgaa
       61 gataaatcaa aaaagaaatt aagcacaaag ctacaggacg atttcgatgc ttatcctgtt
      121 tttcttgcgg aagaaacgat ggaaaaattc taccacggat tttgcaataa aacgatctgg
      181 cctctctttc actactttac ttcctatgtt gaatatgatg aagaatgctg ggaacattac
      241 caacgggtaa atgaggcttt ccgcgatgca gtgatggaaa ttattaaacc ggatgatatt
      301 atctggatac atgactacca ccttatgttg cttcccaaga ttttaagact aaaaatatcc
      361 aaccctatcg gatttttcct gcatatacct tttccaactt ttgaaatatt tcgacttctc
      421 ccgggcacgt ggcgcagaga aatattacag ggtcttttag gcgctgatct tattggattc
      481 catagctacg attatactca gtatttctta agatgtgtac tacgcatttt gggttacgag
      541 cataatatgg gccagatcat cataaatgat cgtattataa aagctgatac atttccaatg
      601 ggaatagatt ttcaaaaatt ttataatgcc gtaaatagtc cggaagttca gaaggagaaa
      661 aacgaatttc aaaagacttt aggcaatttt aagatcatcc tttcgataga ccgtctggac
      721 tataccaagg gtatcctcaa ccgcctacga ggttacgaag cgttccttga caaaaatccc
      781 cagtggcata ggaaagtgat tctcgttttg atagctgtcc cttcccgcat cgggatagaa
      841 cattaccggg agatgaaaag acagatagat gagctcgtag gaaaaatcaa tggcagattc
      901 ggtagtatca actggacgcc tattttatat caatttaaat ttgtaccttt caatcagctg
      961 gtcgcccttt ataatatcag cgatgtagct ttaattactc ctttaagaga tgggatgaat
     1021 ctcattgcca aagaatacat ttcagccaaa gccgatagtg ccggggtttt aatcctcagc
     1081 gagatggcgg gtgcggctaa ggaattaggc gaggctatca tcattaaccc taataacctg
     1141 ccagaaatta cagatgcttt aaagatagcg ttggaaatgc ctaaagaaga acagttaaag
     1201 cgtaatcagg tgatgcagaa acgtttaaaa cgctatgatg tggttaagtg ggcagatgat
     1261 ttcatccaag atttgctatc cttaaaagaa aatcaaaaga atttcaatgc gaaactattg
     1321 agttcggcta tcagaaatcg gcttatcaaa gatttcagcc aagcaaatcg caggcttatc
     1381 attctcgatt acgatgggac attggttccc tttgtcgacc agccccaaat ggcaagcccg
     1441 gatgagcagc ttttagagat tttacaatcg ctggcggaaa atccaaaaaa cgagatcgtt
     1501 ctaataagtg gacgtgataa agataccctt caaaactggt ttggcaagct tgatatcggt
     1561 ttagtagcgg agcatggcgt ctggctcaag gagaaaaaca aagactggga cataattaaa
     1621 ccgttaacca atgattggaa atcaaaaatt attccgctcc ttgaggtata taccgatcgt
     1681 ttacccggtt cttttataga agagaaggaa ttttccgtgg cgtggcatta tcggatggca
     1741 gaacctgaac ttggttcttt acgggcaaaa gaactcaccg attacttatt aaattttatc
     1801 gccaatattg atattcaggt gcaacagggg aaaaaagtgg ttgaaattac taatgcaggc
     1861 ataaataagg gtgccgctgg acttcaatgg attggaaaaa aggattttga ttttatatta
     1921 gcaataggcg atgactggac tgacgaaaac ctattcgaag tgcttcccga aagagcttat
     1981 tcaatcaagg ttggtatggt tgaaactcat gccaggttta gtctctataa tcataccgaa
     2041 gttattgaat tgctcaagaa cttgttgaaa gtcaatggaa actaagaaat ggagagtaat
     2101 atcatattaa agtttagtct cgataaattg gaattcataa taagcctaaa ctataattaa
     2161 ggggataaga aaatggcgaa attaattgac tatcgaggaa ttgtcggcga taaagtaatt
     2221 actggcatat tccaaaaagc acggggttta tatggcaaaa aaattatcca cctgaattct
     2281 acctatatgg gcggtggagt ggctgaaatt ttaaacagtc tcgtaccgtt gatgaacgac
     2341 gtcggtctcg atactggttg gcggatcatc agaggcaatc ccgatttatt taccataacc
     2401 aaaaaattcc ataatgcact ccagggcgat ccgatcaacc tgagcgatat gaaaaaagaa
     2461 atttacatta aagccagcga ggacttttcg gtttttactc atctcgatca cgattgtgtc
     2521 attattcacg acccacaacc gctcccattg attagatact acgtcaaaca acagccctgg
     2581 atttggcgct gtcacgttga tatttcgcat cccaatccgg tattatggga ttttctgaag
     2641 gcgtttatcc tcaaatacga tttagcaatt gtatctcatg aaacttataa aaaagacgat
     2701 atttatatcg aacaaaaggt tattccaccg gtgattgacc cacttagccc taaaaataaa
     2761 gaattgtccg aaaaggatac caagcgattt ttaaaaaaat ttggaatacc aatagataaa
     2821 ccgcttctta ctcaaatctc gcgcttcgat aagtggaaag acccggaggg cgtcgtaaga
     2881 atttttaaaa aagtcaaaaa acagattgat tgccgtttgg ttttatgtgg cagtatggct
     2941 actgacgacc cggaaggtat gaaaatatat cagaaagttg tggactattc taatcatcta
     3001 atccaatccg gggatattat tcccattacc tccgaaaata acattctcgt caattctctt
     3061 cagcgttatt ccgacgtcgt aatccagaaa tctatcagag aagggtttgg attggtagta
     3121 gcggaagcac tctggaaaaa agccgctgta gttgcctcca atgtcggtgg aatacccctt
     3181 caggtcattg acggtgaaac gggttttctt tgcgagccca atgatgaaga ggccttcgcc
     3241 gaacggataa ttcaattaat gaaagataaa aaactgaaag aaaaactggg gcaaaacggc
     3301 aaagagcata tcaaaagtaa cttcctcatt acccgcctgc tttgtgatta tctggatgta
     3361 atcagatcgt tattatcctg acaattcggc aatctcattt atttcattta ttataaaaac
     3421 acgccaagca gaaaatctaa atcgcactta tatcttgagt cttaataatc tgttttggat
     3481 tagcttacga taatgttatt aaaaaacatt ccgtttcctt tacttttaaa atgctttctc
     3541 taaa
//
LOCUS       DAOMJU010000125         3471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_30515_length_3471_cov_25.927400, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000125 DAOMJU010000000
VERSION     DAOMJU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30515_length_3471_cov_25.927400"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLO58_09005"
     CDS             complement(<1..791)
                     /locus_tag="PLO58_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HQO74964.1"
                     /translation="MKKLSVLTLLFFLPVALAAFTGEIIKTYNLPSTCPTGLTYDGQN
                     LWLADRKTDQLYCLDKNDGKVVRQLPAPGYWPMGLAWDGKALWVSDLRDGKIYQIDPK
                     SGLVIKTIYAPGGSPRDLAWDGKYLWVADDGSDEIIQFSPDDGTTIKSFRAPSTEVRG
                     LAFDGHYLWADDRGKDEIYMIDPRSGCVLIVTTAPGPYPKGLAYDGQALWAVDYQTDK
                     LYQLKVQDDQKFIRKNERKAKVTYTHQTRNFGPGKIRNLDVYIALP"
     gene            complement(817..1257)
                     /locus_tag="PLO58_09010"
     CDS             complement(817..1257)
                     /locus_tag="PLO58_09010"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQO74965.1"
                     /translation="MKILIIHGPNLPFLGKISAVTGSRLTLDKVNTSLRRMAQESGIE
                     LKIFQFYSEEKILKAVARNRSDIAGLLISPGALAVNCHALRELLSILKIKTVEVHLSE
                     MPDAKASFGQSALRDIAAARFLEPGLTAYQKGLQYFIKTKDASQ"
     gene            1394..1531
                     /locus_tag="PLO58_09015"
     CDS             1394..1531
                     /locus_tag="PLO58_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74966.1"
                     /translation="MKIKKKILVLIVVFLLMVASLAISIRSNRQSDIPARESANLKIG
                     N"
     gene            complement(1599..2393)
                     /locus_tag="PLO58_09020"
     CDS             complement(1599..2393)
                     /locus_tag="PLO58_09020"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HQO74967.1"
                     /translation="MKIFKNNLLIILASVIAGLAGWQSCDLPWQPGPMPDAIIETEFV
                     PGLNILGIVRLEDTTASSFVYIERAYASNEYETWVDSLPIIKNANVWIAGTRDTTGYI
                     FRYYGEQSDHKYINTDFRPVAGETYQLTITAPDLPDLSASTSVPHLPAIDTGSVVVSE
                     DGVNFNLRTTADAYLYDVYLLCENGQLVNRLSNDGAIVKELQFKLNNNLGAPLAIQVY
                     GYDENMATILNATSSIIPQSFLETLSTVEGGYGCFGAVSVLTYVLP"
     gene            complement(2396..>3471)
                     /locus_tag="PLO58_09025"
     CDS             complement(2396..>3471)
                     /locus_tag="PLO58_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74968.1"
                     /translation="KFGWEQSHLSFLNKIGGQTDTSARRIGADLQAVYIQDKIEIGNL
                     ALKFGGRSSRQMSSESWHFEPRAALALRLGITTVKAAWGHYYQYLTTLDSRNREFIQF
                     LDYYYNLENQKPVESIHYILGVEGFLTDKLEYSVTGYYKDLTRLYRLEYDAAKKSLEL
                     SEILEAGQGKAYGIEWLLRGEVERLSGWVSYTFSHGTRSYPSLQNGRWYYYEGDQPHN
                     LKLLLMYKLTPAITASTTFQFSSGYPRTWETGRYNQYFYNPLENSVGGFPRDITPVKN
                     NVRFPPRIVWDIGWKKKLREGFGQHLSEYLGLKEAYMTMTIRNLLFLRRNPYYYFYFP
                     GYGYYAFGLEYFPSVNFGYTLRF"
BASE COUNT          956 a          795 c          793 g          927 t
ORIGIN      
        1 ggcagagcga tatagacgtc aagattacga attttgcccg gcccgaaatt gcgagtctgg
       61 tgggtataag ttactttagc cttacgttca tttttacgaa tgaatttttg gtcgtcctgc
      121 actttcagct gatagagttt atcggtttgg tagtcaaccg cccagagcgc ttgtccgtcg
      181 taagccagac ctttcgggta tggtcctgga gcagtcgtaa caatgagaac acagccactc
      241 cgcggatcaa tcatatagat ttcatctttg ccgcggtcat cagcccaaag ataatgaccg
      301 tcaaaagcca gaccgcgaac ttcggtggaa ggcgccctga aggatttgat ggtagtgccg
      361 tcgtccggac tgaactggat aatttcatcc gaaccgtcgt cggcgaccca caggtatttt
      421 ccgtcccagg ctaaatctcg cggactcccg cccggtgcat aaatagtttt aataaccaat
      481 ccgctcttcg gatcaatttg gtaaatcttg ccgtctcgta aatccgagac ccatagagct
      541 tttccgtccc aggctaagcc cattggccag tagccgggcg cgggcaattg acgcacgacc
      601 ttaccgtcgt ttttatcaag gcaataaagt tggtcggttt ttcgatcagc aagccagagg
      661 ttttggccat cgtaggtcag tcctgtggga caggtggacg gtaaattata ggtcttgatg
      721 atttcaccgg taaaagccgc taacgcaacc ggtaagaaaa ataatagggt aagaacgcta
      781 agcttcttca taaaaaacct ccagtattga caggtttcat tgtgacgcat ctttggtttt
      841 aataaaatat tgtaagcctt tttgataggc agttaaaccg ggttcaagga aacgcgcggc
      901 ggcgatgtcc cgcaaagcgg attgaccaaa gcttgccttc gcgtctggca tttcgctcag
      961 gtgaacctcg accgttttga ttttcagaat ggaaagcagt tcgcgaagag cgtggcaatt
     1021 gactgccaga gctcccggac tgattaataa tccggcgatg tcagaacggt tacgagcgac
     1081 ggctttcaat attttttctt cggaataaaa ttgaaatatt ttcagttcaa tgccggattc
     1141 ctgtgccata cgcctcaggg aagtgttgac tttatctaat gtcaagcgcg agccggttac
     1201 ggctgagatt ttacccagaa agggcagatt gggtccgtga attattaata ttttcatact
     1261 gaaaaataag cgggatttgg ctgaattgca aatgtgatga aagtcaaatc gtaacgcaga
     1321 tttgtcgctc gacacagatt aactttcacc ggttcattaa aatttaagta tcttgaattg
     1381 acacagtaga tttatgaaaa taaagaaaaa aatattggtt ctaattgtcg tatttttgct
     1441 gatggtagca tctcttgcta tcagcattcg cagtaatcgg caatcggaca tccccgccag
     1501 agaatctgcc aatctcaaaa tcggcaatta aaaaccgcat ttttacagtc ggtgagatta
     1561 caccgattct attatttgga ttgtcataac taaaactttt aaggcaaaac gtaagttagc
     1621 acactgaccg ctccgaaaca gccatagccg ccttcgaccg tgctaagggt ttccagaaaa
     1681 gattgtggaa ttatgctcga agtggcattc agtatcgtcg ccatattctc atcgtagccg
     1741 taaacctgga tcgccagtgg agcgccaagg ttattgttga gtttaaattg caattccttg
     1801 acaattgctc cgtcattcga gagtctattg accagttgac cattttcaca caacaaataa
     1861 acatcataca gataggcgtc ggcagttgtg cgcaaattga aatttactcc gtcttcggaa
     1921 actacaaccg agccggtatc aattgccggc aaatgaggaa cgctggtgct ggcagatagg
     1981 tcgggcaaat ccggcgccgt gatggtcaat tgataggttt caccggcgac tgggcggaaa
     2041 tcagtgttga tgtatttatg gtcgctttgt tcgccgtaat aacggaaaat atagccggtg
     2101 gtatcgcgcg tacccgctat ccatacgttg gcatttttga taatcggtaa ggaatccacc
     2161 caggtttcgt attcattgga ggcatacgcc cgctcgatat aaacaaaaga gctcgccgta
     2221 gtatcctcga gccgcacaat ccccaaaata ttcaagcccg ggacgaattc cgtctcaatg
     2281 atcgcgtccg gcattggccc gggttgccaa ggcaaatcac aactttgcca gcccgccaga
     2341 ccggctatga ccgacgccaa aattatcaat aaattatttt taaatatttt cataattaaa
     2401 accggagcgt atatccaaaa tttacactgg gaaaatattc caatccgaaa gcataatagc
     2461 cgtatcccgg aaaataaaaa tagtagtaag gattgcgccg caaaaataac aaattgcgaa
     2521 tagtcatagt catataagcc tctttcaatc cgagatattc cgacaggtgc tgaccaaatc
     2581 cctcgcgcag ttttttcttc cagcctatat cccagacaat gcgcggcgga aagcggacgt
     2641 tgttcttcac cggagttata tcgcgcggga aaccgcctac gctattttcc agcgggttat
     2701 aaaaatattg gttataacgc ccggtttccc aggtgcgtgg ataaccggaa ctgaattgaa
     2761 aagtcgtgct ggcggtgatt gccggggtca atttatacat taacaaaagt ttaagattat
     2821 gcggctgatc gccttcatag taataccatc gtccgttttg caatgatggg taactgcgag
     2881 taccgtgaga aaaagtatag ctaacccatc cggaaaggcg ctcgacttca ccgcgcaaaa
     2941 gccattcgat tccgtaagcc ttgccctgcc cggcttcgag aatctcagac agttcaagtg
     3001 attttttagc ggcgtcatat tccaggcgat aaagccgagt taaatctttg taataacccg
     3061 taaccgaata ttccagtttg tcggttaaaa agccctcgac tccgagaatg taatggatag
     3121 attcgaccgg cttctggttt tccagattgt agtaataatc caggaattga atgaattctc
     3181 gattgcgtga gtccagagtc gtaaggtact gataataatg tccccaggcg gctttgacag
     3241 ttgtgattcc caatcgcaga gccagagcgg cacgtggttc aaaatgccaa ctttctgatg
     3301 acatttgccg cgaactgcgc ccaccgaatt tcagcgccag attgccgatt tcgattttat
     3361 cctgaatgta aaccgcttgt aaatcggctc caatacgacg tgccgaagta tcagtctgcc
     3421 caccaatttt atttaaaaaa cttaaatggc tttgctccca accgaacttg a
//
LOCUS       DAOMJU010000126         3425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_31111_length_3425_cov_23.396736, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000126 DAOMJU010000000
VERSION     DAOMJU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31111_length_3425_cov_23.396736"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PLO58_09030"
     CDS             <1..990
                     /locus_tag="PLO58_09030"
                     /inference="COORDINATES: protein motif:HMM:NF028498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HQO74969.1"
                     /translation="YLKSNNNVVGGTLKKVAGKFVYEGFADWWYESLMAYANAGATID
                     YISIQNELNYEAPWESCVFAPSESDDTTLAAHNVAYETVWQKLNAEMGKTMPKMLTPE
                     TSGLGDSEVYIKNLIDLSHVYGYAHHLYDCSGCGADPDRFIPRMISYREMVKKYGAKP
                     IFQTEFEDDPGAWPDALNTALLLHNSLTVEQVSAYLYWDLFWAPGSGLISLNDFDSYT
                     IKPTYYAFKQYSAFIDANWQRVEVTNDNTGLRISAYVSPDNKKMTIVILNITQDMDIK
                     LKLAIKNFASAEGAIYRTSETENCVYVGEFNKRKPLVLPANSITTLSLSTAEK"
     gene            1083..3110
                     /locus_tag="PLO58_09035"
     CDS             1083..3110
                     /locus_tag="PLO58_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQO74970.1"
                     /translation="MKSVRHTIANGGLNRQHLQYQTSALVLVILLIMGFLSFVSAEPT
                     KWVGTWSTAPQLVETNNNPPSPGLSNNTLRQIVRVSIGGDTLRLRFSNEFSTKPVTMN
                     SVHVAISTGGGAIDTATDKPLFFNGSPNVTMEAGKAVTSDPFQFTLPPRADIAITIYF
                     GATSPDVTGHPGSRTTSYLLTGNAVTRADCAGAVTTDHWYVINTIDVKAPDSAAAVVI
                     LGNSITDGRGSGTNKQNRWPDELARRFQENPATRFVAVLNQGIGGNCVLSGGLGPTAL
                     SRFERDVLNQSGVRWLIIFEGINDIGNGAPGVGNKLIDAYKQMIVNAHSRGIFVYGAT
                     LLPMKGSFYYSTAREAERQIVNDWIRTSGFFDGVIDLDRALRNPADTLSLLPEADSGD
                     HLHPNEVGHRLIAEAVDLNLFIGREPLDFTDESMRIYFEPECAIVGENWDILTDSQAS
                     NGKYVTVKAGMQSLDSAPSGDTNAIHIPFSVDSAGSYYLYARLNCPSYDDDSYWVKMD
                     GGNFQMFNGLVTTGWEWKNLSSYTLTKGEHKLTITYREDGAKLDKICLSNSAYAPIGM
                     GDEAENICSPAGMGLDNSLKVPDRYALRSNYPNPFNPTTTIAYQLSETAFVRLAVYNM
                     NGQLVENLIAKEQSAGYYDTVWNATNYSSGIYLYKFQAGNFQQVGKCLLLK"
BASE COUNT          954 a          767 c          785 g          919 t
ORIGIN      
        1 tatttgaaga gtaataacaa tgtggtaggt ggaacgctca aaaaggtcgc cggaaaattc
       61 gtttacgaag gatttgccga ctggtggtat gaaagtctga tggcttatgc caatgctggg
      121 gcgaccatag attatatcag cattcagaat gaattgaatt acgaagcgcc ttgggaatcg
      181 tgtgtatttg ctcccagcga atccgacgat accacgcttg ccgctcataa tgtagcctac
      241 gaaaccgtct ggcaaaagtt gaacgctgaa atgggcaaaa ccatgccgaa aatgcttacg
      301 cctgaaactt ctggcctggg agattctgaa gtatatatca aaaatttaat agatttgtcg
      361 catgtctatg gctatgctca tcatctttac gattgtagtg gatgtggggc agatcccgat
      421 cgcttcattc cgcggatgat tagttatcgg gaaatggtca agaaatatgg cgccaaaccc
      481 attttccaga cggaattcga ggacgacccc ggcgcctggc cggatgccct caataccgcg
      541 ctattgttgc ataattccct gacagtagag caagtttctg cctatctgta ctgggattta
      601 ttctgggcgc ccggttccgg tctgattagt ctgaacgatt tcgattccta cacaatcaaa
      661 cccacttatt acgcctttaa acagtattca gctttcattg acgctaattg gcagagagtc
      721 gaagtcacca atgataatac cggccttaga atttccgcgt atgtcagtcc ggataataaa
      781 aaaatgacga tcgtgattct caatataact caggatatgg acattaaact taaacttgcg
      841 atcaaaaatt ttgccagtgc cgaaggcgca atctatcgca ccagtgaaac cgaaaactgc
      901 gtctacgttg gtgaattcaa taaaagaaag cccttagtat tgccagccaa ctctatcacg
      961 accctctcgc tttctaccgc ggaaaagtaa ctgagattaa ctgccaaaga gcggatatct
     1021 ctcaaatgtc gcttcacagt tgaaaaattt gattttccca atatgaaagg agaaaatgct
     1081 atatgaaatc ggtaagacac actatcgcta atggcggact taatcgccag catcttcaat
     1141 atcaaacttc tgcccttgta ttggtaattc ttttaattat gggatttctt tcctttgttt
     1201 ctgctgaacc gaccaaatgg gtgggcacct ggagcactgc cccacagttg gtggaaacaa
     1261 acaataatcc gccaagcccc ggcttgagta ataataccct gcgccaaatt gtccgcgttt
     1321 ccatcggtgg tgacaccctg cggctacgct tttccaatga attcagcacc aaaccggtca
     1381 cgatgaattc agttcacgtc gctatctcaa ccggcggtgg tgcgattgat accgccacgg
     1441 acaaaccgct atttttcaac ggctctccga atgttaccat ggaagcaggt aaagccgtta
     1501 cctcagatcc cttccaattc accttgccac ccagggcgga cattgcaata actatttatt
     1561 ttggcgcaac ttctccggac gtaaccggtc accccggctc gagaactaca tcatacctac
     1621 tcaccgggaa tgcagttacc agagcagatt gcgccggtgc agtcacaact gatcactggt
     1681 atgttattaa taccattgat gtaaaagcgc cggactcagc cgcggcggtg gttattttgg
     1741 gtaattccat cacagatggg cgcggctccg gcacaaacaa acaaaaccgc tggccagatg
     1801 aactggcgcg tcgctttcag gaaaatccgg ctacccgttt cgtagcggtt ttgaatcagg
     1861 gaattggcgg taattgcgtg ctcagcggag gcttgggacc gaccgcgctt agtcgctttg
     1921 aacgcgatgt actcaatcaa agcggagtga ggtggctgat cattttcgag ggtataaatg
     1981 acattggcaa tggggcgccc ggcgtgggca acaaactaat tgacgcttac aagcagatga
     2041 tagtaaatgc gcattccaga ggtatctttg tgtacggagc aacgttatta cccatgaagg
     2101 gctcatttta ttattctaca gctcgtgaag ccgagaggca aattgtcaac gattggataa
     2161 gaaccagcgg tttcttcgat ggagtcatag atttagatcg ggcactgcgc aatccggcag
     2221 atactttaag tctattacct gaagcggatt ctggagatca cctccacccc aatgaagtcg
     2281 ggcatcgatt gatagccgaa gcggtggatc taaatttgtt cataggcaga gaacccttgg
     2341 attttaccga tgaaagtatg agaatttatt tcgagcccga atgtgctatc gtaggagaaa
     2401 attgggatat attgaccgac tctcaggctt ccaatggtaa atatgtgacc gtcaaagccg
     2461 gaatgcaaag tctcgatagc gcgccctccg gtgacacaaa cgccattcat attccttttt
     2521 cggttgatag tgccggaagt tattatctgt atgcgagatt aaactgcccc tcctatgatg
     2581 atgattctta ttgggtaaaa atggatggtg gtaattttca aatgtttaac ggattagtga
     2641 cgaccgggtg ggaatggaag aatttaagtt cttacactct gacgaaaggc gagcataaac
     2701 ttacgatcac ttaccgcgaa gacggagcaa agctggataa aatttgtctt tcaaattccg
     2761 cctacgcgcc gattggtatg ggagacgagg ccgaaaacat ttgtagtcct gccggaatgg
     2821 gactggataa ttcactcaaa gtgcccgatc gctatgctct cagatcaaat tatcccaatc
     2881 cttttaatcc caccacgacg atcgcttatc aattgtccga gacagccttt gtcagacttg
     2941 ctgtatataa tatgaatggt caattagtcg aaaacctgat tgctaaagaa caatcggccg
     3001 ggtattacga tactgtctgg aacgccacga attatagttc gggaatatac ttgtataaat
     3061 ttcaggcagg caattttcaa caggtcggga aatgtttatt attgaaataa gtcctcaaaa
     3121 tacctgactg tttacgatct caaattgttg gggctgtaga atatttggtt ttacctttcc
     3181 tcaaaaatta ccgatggtca ggcaacttaa aactgaatgg ggaagggttt ggatacgtga
     3241 tgttttcttt cgagataaag cgctttcgaa gttcggctta atccaaatcg tcggacgaca
     3301 acttaagtca aacgctaccc tttctcctct gcttatcttt tctctggctt gacctttttc
     3361 tcgagtcttt gtggcataag attaaaccgc taagttcgct aagagacgta aaggaaaata
     3421 gaaaa
//
LOCUS       DAOMJU010000127         3332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_32418_length_3332_cov_12.908453, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000127 DAOMJU010000000
VERSION     DAOMJU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32418_length_3332_cov_12.908453"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..3131)
                     /locus_tag="PLO58_09040"
     CDS             complement(180..3131)
                     /locus_tag="PLO58_09040"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQO74971.1"
                     /translation="MTQSLWAKGTIRGRVFDKNTKDPLSYANIMIKGTTIGTAADADG
                     MYSITNAPDGPQTIVISYIGYVTLEVDVNIPDGGTVRRDFGLEPEVLQGKEVIVTAQA
                     LGQMQAINQQLASDKIASVVSEARIQELPDFNAAQALSRLPGVSTLESSGEANKVVIR
                     GLAPQYNAVAVEGIKLASTGSTQIGVSALGNTAGSISNDRSVDLSMISPYMIKSISVF
                     KSLTPDMNANSIGGSVNMELREAPSQLHYDLMWQSGYTAKTNNYGNYRAVGSISQRFF
                     QDNLGAYLLINAEKYDRDADNMSANYRVTSSVIDTSTGYRPVSVNSVTLNRHVETRKR
                     YGGNLILDYKLPAGSIKSVNMYTRFVSDYQDYNTVLNYFDKILNFNYRGGINTTDLSV
                     NSLELKYDFRYIQMDLKAANTHSRNDLPESPYITFKTGQAMMGSVPINTVPDSLTRLW
                     VYPGHDKVYLDYLNLFSSLYTENNDNFSSDFKVPFSIGKSLLGYFKFGGQYYNQKNTN
                     DQKTPYAEIRKSGEFQAAMVEDLERQFGIPVDPSTAKFAASNFTGSSKLMQPFLDNQF
                     GEIYWACKPDILVEMADYLNNNPQWRGKATGGADQTGGWYNGMFQNLANSYRYREEYY
                     AGYVMSEIDFRNLMIVGGARYEKTESKYTAYNMYDMRNPDAQNCDTVYSYPKSEFWLP
                     MVQAKYSPFEWFDIRYAYTQTLARPDYHQMSPKINYDNTRRSVRAGNPDLVPAHAYNH
                     DLNLTFHGNKLGLLSIGGFYKTIKDFVYSTAYILHKTSKSSDIKTINDIQIMGIKPEE
                     GATLYTYINSRYLAYVKGLEFDWQTRFWYLPWGLDGVVLGVNYTMIESEATYPLREEV
                     TDYTVRPPITTTRDSTRTGRLLYQPNDVVNAYIGYEYKGFSARLSFLFQGNSVSYVGT
                     FPEQDGYTRDYFRIDASVRQTLPWLGSEIYLDVNNLNNEVNSSAQMSIDGFTNVKHYG
                     MTANLGIRFRL"
BASE COUNT          995 a          744 c          614 g          979 t
ORIGIN      
        1 aaccaccacc ataaataccg tttacttcta caacagcctt ctcttgtgca tttagtccta
       61 cagtcagaaa aattgcacaa agtaaaaata ctaacattct tttcatgttt ttgacctcct
      121 gaattttttg tttcgagtat tttggcatta tttttaaaaa tattcctttc ataattgtct
      181 tataatctga atctgatccc aagattagcc gtcataccat aatgttttac attagtaaag
      241 ccatcaatgg acatctgagc cgatgaatta acttcattat tgagattatt gacatccaaa
      301 taaatttccg aaccaagcca cggtagagtc tgtcgaacgg aagcatctat ccggaaataa
      361 tctctggtgt atccatcttg ctccggaaaa gtacctacgt aactaacgga attaccctgg
      421 aacaagaatg ataaccgggc tgaaaagcct ttatattcat aaccgatgta ggcgttaacc
      481 acgtcattcg gctgatagag caaacgtccg gtacgagtgg aatcacgagt cgtagttatc
      541 ggcggcctaa cggtataatc agtcacttcc tctctcaaag gataagtggc ttctgattcg
      601 atcatcgtat agttaacacc cagaactacc ccatccaaac cccatggtag ataccagaat
      661 ctggtctgcc agtcaaattc gagacctttt acataagcca gatagcgact attaatatag
      721 gtatataaag tagctccttc ctccggttta atccccatta tctgaatatc attaatcgtc
      781 ttaatgtcgc ttgatttgga agttttatgg aggatataag ccgtactata aacaaaatcc
      841 tttatggttt tataaaaacc accgattgaa agaaggccta acttattacc gtgaaaggtc
      901 aagttcaaat cgtgattata agcatgcgcc ggtactaaat cgggattacc ggctcgcaca
      961 cttcttctgg tattatcata gtttatttta ggcgacatct ggtgataatc cggtcgagca
     1021 agggtttgag tgtaggcata acgaatatcg aaccactcga aaggtgagta tttagcttga
     1081 accattggca accagaattc gctttttgga taagaataga ccgtatcgca attctgagca
     1141 tcagggttgc gcatatcgta catattgtag gcggtatatt tagactcggt tttttcgtac
     1201 cgggctccgc caactatcat taaattcctg aagtcaatct ccgacattac ataaccggca
     1261 taatattctt ccctatatct atatgaatta gctaaattct ggaacattcc attataccaa
     1321 cctccggtct gatcggctcc accggtagct tttcctctcc attggggatt attattcaaa
     1381 tagtctgcca tctccaccag aatatccggc ttacaagccc agtagatttc tccaaattga
     1441 ttgtccagaa aaggttgcat caatttggaa ctgccggtaa aattggaagc cgcaaatttg
     1501 gcggtactgg gatcaacagg aataccaaac tgtctctcca aatcttcgac catcgccgcc
     1561 tgaaactcac cacttttcct gatttcggca taaggtgttt tctgatcatt agtattcttc
     1621 tgattataat actgaccgcc gaatttaaaa taacctaaaa gagacttccc aatactaaaa
     1681 ggaactttga aatcggaaga gaaattatca ttattctccg tatataaaga actgaacaaa
     1741 ttcaagtaat ctaaataaac tttgtcatgg ccaggataaa cccataatct ggtcaaacta
     1801 tccggaacag tattaatcgg gacgctaccc atcattgctt ggccggtttt gaaagttata
     1861 tagggagatt ctggcaaatc attccttgaa tgagtattgg ccgcttttaa atccatttga
     1921 atatatcgaa aatcatattt taattccagc gaatttaccg acaaatcagt cgtatttatt
     1981 ccacctctat aattaaaatt gagaatttta tcgaaataat ttaaaacggt attataatcc
     2041 tgataatcgg agacaaagcg ggtatacata ttcacggact taatcgaacc ggcaggcaat
     2101 ttataatcta aaattaaatt accaccataa cgcttgcggg tttcaacatg tcggttaagg
     2161 gttacactat taacactaac cggtcggtaa ccagtagaag tatcaatgac actactagta
     2221 actcgatagt tggcgctcat attgtcagca tctcgatcat atttctcggc gttaatgagg
     2281 agatatgctc ccagattatc ctggaaaaag cgctggctaa tggagccgac cgcccggtaa
     2341 ttgccgtaat tgtttgtttt ggcggtgtag ccggattgcc acatcaaatc ataatgcagt
     2401 tgtgaaggcg cttccctcaa ttccatattt accgacccac caatggagtt ggcattcata
     2461 tccggagtaa gcgacttaaa aaccgaaatg gatttaatca tataaggcga gatcatcgag
     2521 agatcaacac tgcggtcatt cgaaatactt ccagcagtat tgcccagagc ggaaactcct
     2581 atctgagtgc taccggttga agccagtttt atgccttcaa ctgccacggc gttatattgc
     2641 ggtgccaagc cccggatgac cactttattg gcttcgcctg aactctccag agtcgagacg
     2701 cccggcaagc gactaagagc ttgagcggca ttaaagtccg gcaattcctg aatacgagct
     2761 tcggatacaa cgctggcaat tttatcagag gctaattgct ggttaatggc ttgcatttgc
     2821 cccaaagcct gagcggttac aataacttct ttaccttgta gaacttccgg ttccaaaccg
     2881 aaatctctcc gcaccgtgcc accgtctgga atatttacat caacttcgag tgttacataa
     2941 ccaatataag aaatcacaat cgtctgcggc ccatccggag cattcgtaat agaatacata
     3001 ccatcggcat ccgccgccgt tcctatggtt gttcctttaa tcattatgtt ggcatacgat
     3061 agcggatctt tggtattttt gtcaaacact ctccctcgga ttgtaccctt agcccaaaga
     3121 gactgggtca ttaggcacag aactaagccg gcgataaaaa atgctttaca cccacgacct
     3181 cgggtcatct taataaaacg cacagaaatc ttcataagac tctatccttt ttactgtttc
     3241 gtaaattttt ttcatagtcc cacatatcta ttagatgtct tatcaattac cttctcaatt
     3301 ttaaagatat aatttatgaa aggcaactgc gt
//
LOCUS       DAOMJU010000128         3277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_33179_length_3277_cov_20.495965, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000128 DAOMJU010000000
VERSION     DAOMJU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33179_length_3277_cov_20.495965"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1036
                     /locus_tag="PLO58_09045"
     CDS             62..1036
                     /locus_tag="PLO58_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQO74972.1"
                     /translation="MSKTFISDLKENDRVTSFFGVFSKAFRRTKENKIYLDLILVDRT
                     GKINAKIWDDVENLAEQFEAGDAVKVQGTVTIFNNELQIKVEKIRRVIPERDRAEGYD
                     AGDLILSTSKNIESMWEDLQQIIQSLKNPFLKAVVGAIYEKYGEQLKIHPGSMILHHA
                     FRGGLLEHIYTMCQVAEGVCQAYTELDRDLVMSGILLHDIGKLVELEAGMAVSYTDAG
                     YFLGHIVLGRDIVRDQCLAINGFPEILRLQLEHILLSHQGKHEFQSPREPQFLEALVV
                     YYIDELDTRFNQMKNDIATDLNEGKWTAKGGYFKRVLFKGERESDQNS"
     gene            1094..1654
                     /locus_tag="PLO58_09050"
     CDS             1094..1654
                     /locus_tag="PLO58_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74973.1"
                     /translation="MNSRIRKITYSAIFIALIVGVGYALAFVPNVELVTALIFLSGVL
                     MGWKSGLIIGGLGEFLFSALNPLGSGLIFPPMLIAQVLAMVIIGGVGGLCRNFVLSWQ
                     PRLRNFIAIGLLGGLLTIFYDICVSIAYPLSAGFNWRGLVTTLAAGLAFSIIHIIVNL
                     IVFLFLVPVTAQRIARAIPYFREERR"
     gene            1656..>3277
                     /locus_tag="PLO58_09055"
     CDS             1656..>3277
                     /locus_tag="PLO58_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74974.1"
                     /translation="MKALRLLLIGGWVAIFIESGISENQPQTDSSRVETETQSVGTYN
                     IGDHIFERSNYIFLPYDDWGVKGVIQTFGLPVTALTMQNNSLSMTDPVYGQAPLTWLN
                     PRFDQIELEATAIKMTPRYFLRDKVFSRFDYYRGDYGFMNFSMLISGKLADTTTVWRF
                     VGENLGYDSYYGNLGAKLSQTGESLSQIYRLDLRAFWKSWQIDLNAAYQKYLPGYVKV
                     SPTTSSAYLSPSWNGNVKEYRAALGGIAIRASERDSTALGFQQNNFIYGQYVKDPRFG
                     FKGVADQSEFIFYHDRQISHFRLAMHFNPVTRSVTLRNGFYERQNLAAGGIGISSKKI
                     KSHWSLYLGAIDQYFDGEFHLRYHLSPKNQVGGRIASTYALYPLIYHLDFPVNQPISK
                     EGFQYRIGTIDFLHASRSLQLSLAFNQVNSNFYVPYKSAINDTTLIYLPQKLDAAYIT
                     ADGRLILPWRMTLTVRGLISNNEEGVWLQGWGQIRQEIDLFQNNLRLYVAGELTYWDM
                     SKNLGWFEELRGIGQFGTEYFTNNQLNIVARIG"
BASE COUNT          893 a          633 c          776 g          975 t
ORIGIN      
        1 aaaaattact tgactttggg agtctaatta gctttaattc tggaaaattc agagggaaaa
       61 aatgagcaag acatttatta gcgatctcaa agaaaatgac cgggtcactt cgttttttgg
      121 cgtattctcg aaggcatttc ggcggacgaa ggaaaataaa atttatctcg acctgatctt
      181 agttgaccgc accggcaaaa taaatgctaa aatctgggac gatgtggaga atttagccga
      241 gcaattcgaa gctggagacg ccgtcaaagt tcaaggcaca gtcacaattt ttaacaatga
      301 acttcagatc aaggtggaaa aaattcgacg cgttatcccg gagcgtgacc gcgccgaagg
      361 ctacgatgcc ggtgatttaa tacttagtac gagtaaaaat atcgaaagta tgtgggagga
      421 tttacaacaa ataatccaat cgttaaaaaa tcccttcctc aaggcggtcg ttggcgctat
      481 ttatgaaaaa tatggcgaac agttgaaaat ccacccaggc tcgatgattc tccatcatgc
      541 tttccggggc ggattattgg aacatatcta tacaatgtgt caggtagcag aaggagtttg
      601 tcaagcctat acagaactcg atcgcgattt ggtaatgagc gggattttgt tgcacgacat
      661 cggcaagtta gttgagttgg aggctggaat ggcggtatct tataccgatg ccgggtattt
      721 tctcggtcat attgtattgg gacgagatat cgtccgtgat caatgcctag caataaatgg
      781 ttttcctgaa attttacgtt tgcaactcga gcatattctc ctttctcatc agggcaagca
      841 cgaatttcaa tcgcctcgtg aacctcaatt tttagaagca ttggtagttt attatatcga
      901 cgaactcgat acgcgcttta atcaaatgaa aaatgacatt gcaactgacc ttaatgaggg
      961 taaatggact gccaaaggtg gttatttcaa acgggtatta ttcaaaggcg aacgcgaatc
     1021 cgaccagaat agctaaagaa tccttgaatt atcggcgtcg ctttgtaaat tgcagcgact
     1081 taatggtggt tttatgaatt caagaattag aaaaataact tactcggcaa tttttatagc
     1141 actgatcgtc ggggtcggct acgcgctggc attcgttccg aatgtcgaat tagttacggc
     1201 tctgatattt ttatccgggg ttttgatggg ctggaagtcc gggctgatta tcggcggatt
     1261 gggcgaattt ttgttctcgg ctcttaatcc attgggctcg ggactaattt ttccgccaat
     1321 gttgattgct caagtgctgg cgatggtcat tattggtggt gtcggcggac tttgccgaaa
     1381 ttttgtacta agctggcaac cgcgactgag aaattttatt gcaattggat tactcggtgg
     1441 gcttttgaca attttttatg atatttgcgt ttctattgcc tatcccttat cagcgggatt
     1501 taattggcgt gggttggtga cgaccttagc ggctggtttg gcattcagta ttattcacat
     1561 aatcgttaat ctgattgttt ttctgttttt agttccggta acggcacagc ggatcgctcg
     1621 agcaatacct tatttcaggg aagaaagacg ctgaaatgaa agctctcagg cttttgctaa
     1681 ttggcggttg ggtagcaatt tttatagaat ccggtattag tgaaaatcag ccccagacgg
     1741 attccagccg ggtagaaact gaaacccaat cggttgggac ttacaatatt ggcgaccata
     1801 tttttgagcg aagtaattat atttttctgc cttatgacga ttggggagtc aaaggcgtaa
     1861 tccagacttt cggtttgccg gttactgctc taactatgca aaataacagt ttgtcaatga
     1921 ctgatccggt ttacgggcaa gcgccattaa cctggctgaa tccgcgtttc gatcagattg
     1981 aattggaagc gactgccata aaaatgacac cgcgctattt tttgcgggat aaagtatttt
     2041 cgcggttcga ttattaccgg ggcgactatg gctttatgaa ttttagtatg ttgattagtg
     2101 gtaaattagc tgatactacg acggtctggc gttttgtagg cgaaaatctg ggctatgata
     2161 gttattatgg taatctcgga gcaaaattat cccagaccgg cgagagtctt tcgcaaattt
     2221 accgactgga tctgcgggct ttctggaaaa gctggcaaat tgacctgaat gccgcttatc
     2281 aaaaatatct gccgggttat gttaaagttt ctcctactac atcctcggct tatttgtcgc
     2341 cttcctggaa tggcaatgta aaagaatatc gcgccgcact gggtggtata gctatcagag
     2401 caagtgaacg cgattcaacc gcgcttggtt ttcagcaaaa taattttatt tacggacaat
     2461 atgtcaaaga tcctcgattt ggattcaaag gcgtggctga tcaatcggaa tttatatttt
     2521 atcacgaccg gcagatttcc cacttccgct tagcgatgca ctttaatccc gtaactcgct
     2581 ctgtaactct ccgtaatggc ttctacgagc gtcaaaattt agcggctggt ggcattggca
     2641 tttcatcgaa aaaaattaaa agtcattgga gtctttactt aggtgcgatc gatcaatatt
     2701 tcgacggcga atttcatctc cggtatcacc tgagtccgaa aaaccaagtc gggggacgga
     2761 ttgcctcgac ttatgccctt tatccgctaa tttaccattt ggattttccg gtgaaccagc
     2821 cgattagcaa ggaaggcttc caataccgga tcggaaccat tgactttcta cacgccagtc
     2881 ggagtttaca attaagtttg gcatttaatc aagtgaactc gaatttttat gttccctata
     2941 aatcggcaat caatgacacg actttgattt atctgcccca gaaattggac gcggcttaca
     3001 taaccgccga tggaagatta atcttgccat ggcgtatgac gctaacggtg cgcggtttaa
     3061 tttccaataa cgaggaagga gtctggttgc aaggctgggg gcaaatccga caggaaattg
     3121 accttttcca gaataatttg cgattgtatg tcgctggaga attgacttat tgggatatgt
     3181 cgaaaaattt ggggtggttt gaagaactgc gaggaatagg ccaattcgga acggaatatt
     3241 ttactaataa ccaattaaat atagttgccc gaattgg
//
LOCUS       DAOMJU010000129         3166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_34779_length_3166_cov_26.265831, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000129 DAOMJU010000000
VERSION     DAOMJU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34779_length_3166_cov_26.265831"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1047
                     /locus_tag="PLO58_09060"
     CDS             199..1047
                     /locus_tag="PLO58_09060"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HQO74975.1"
                     /translation="MPYLGCHVSIENSLEAAPMRGRELGCDAIQIFTSNQMQWKGVPV
                     ADETAEKFLNYQAKHKIAMVIAHDSYLVNLASPEPVKLAMSRKAFIEEIRRSQALKIP
                     YIVFHPGSHMGAGVDFALRKIAESLDYAIEKTPDCRPMFLLETTAGQGTNVGNTFEEL
                     RQIIDYSAHPERLGICFDTCHAYAAGYDLVSPAKYDETFARFDDVIGLSRLKCFHLND
                     SKKPLGSRVDRHANLGDGFLGWEVFYRLINDDRFGNLPMILETPGGDENFAKEIASLK
                     KALEKK"
     gene            1060..2454
                     /gene="lpdA"
                     /locus_tag="PLO58_09065"
     CDS             1060..2454
                     /gene="lpdA"
                     /locus_tag="PLO58_09065"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQO74976.1"
                     /translation="MDNFDLIVIGSGPGGYVAAIRAAQLGMKVALVEKAELGGICLNW
                     GCIPTKALLKNAEVWQNLRTLDEFGITIAQPQLDFTQVIRRSREVAARLSKGIEFLFK
                     KNKVAVVRGYGELRDRQTVAVINPDDNSTTGLKARNIVIATGARPRSIPNVEIDGKRI
                     ISSREAMILPQIPKSIIIIGAGAIGVEFAYLFNAFGVEVNLVEILPQILPIEDEEITK
                     ELSRSFRKRQIGIHINTKVKSVQTQGDFVEVALNSGGESQTLVADYALVATGVQPNSD
                     NLGLAELGVSTEKGWIKVDEHFRTNIPDVYAIGDVIGNPCLAHVASAEGKHAVEFIAG
                     KNPAPVNYAAIPSCVYCQPQVASVGLTEKRASELGYELKIGRFMFRANGKAVASGESD
                     GFVKVIFDGKTQKLIGAHIIGTEATELLPELVLAIDRGLNYSEIKDSIHAHPTLSEAL
                     IEAVRDAFGESVHQ"
     gene            2529..>3166
                     /gene="lipB"
                     /locus_tag="PLO58_09070"
     CDS             2529..>3166
                     /gene="lipB"
                     /locus_tag="PLO58_09070"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQO74977.1"
                     /translation="MPSAVKILDVLDLGLCPYGEAWEIQKKLHAERLASQIPDTLILV
                     EHPNVYTLGKNADARHLLASQEYLKKRGIEVYQIDRGGDITYHGPGQLVGYPIFNLKE
                     HKTSIAWYVNSVEEVLIKTLVKFDIHAERIKGLTGIWLANQKIAAIGMRVSHWVTMHG
                     FALNVTTDLSLFGGIVPCGLATKGVTRMCDLNSAVTMDAVKAVIIKEFVAQFG"
BASE COUNT          846 a          718 c          802 g          800 t
ORIGIN      
        1 gaggagataa tttctatcct gtcaggattt tgtctgacct tattatcgca aggtaactat
       61 tgcggaattc tttcgattaa tccgaatttt atctaaattc gcgcgaacaa atggaacttg
      121 ctcttattac aataaaaata cttcgcagtt agccgtgaaa atagaaccgc aattaaccac
      181 tgactcgtaa ctaaaaaaat gccttatctc ggttgtcacg tctcgatcga aaacagtctg
      241 gaagcagctc cgatgcgcgg ccgtgagcta ggctgtgacg cgatccagat ttttacttcc
      301 aatcaaatgc aatggaaagg cgttccggtt gcagacgaaa cggcggagaa attcctgaac
      361 tatcaagcca aacataagat tgcgatggta attgctcacg actcttatct ggtcaatctg
      421 gcaagtccgg agccggttaa attggcgatg tcgcgcaagg cttttattga ggagattcgg
      481 cgttcgcaag cattaaaaat tccgtatatc gtttttcatc ccggttcgca tatgggtgcc
      541 ggtgtggatt ttgctttgcg taaaatcgcc gagagtctcg attacgccat tgaaaaaacg
      601 ccggattgtc gtccgatgtt tctactggaa acgactgccg gtcagggcac gaatgttggt
      661 aacactttcg aggaattgcg ccagattatt gattattctg ctcatccaga acgactgggg
      721 atttgtttcg acacctgtca tgcttatgcg gctggatacg atctggttag tccggcaaaa
      781 tacgatgaaa cttttgccag attcgacgac gtaatcggtt taagccgact gaaatgcttt
      841 cacctaaacg atagcaaaaa accgctcggg tcgcgcgttg accgccatgc caatcttgga
      901 gacggttttc tgggctggga agttttttat cgtttgataa atgatgaccg ttttgggaat
      961 ttgccgatga ttctggagac gcccggcggc gacgagaact ttgctaaaga aattgcctct
     1021 ctgaaaaagg cattggagaa aaaataataa ggaataacca tggataattt tgatttgata
     1081 gtcattggat caggaccggg cggatacgta gccgctatcc gcgccgccca gctcggaatg
     1141 aaggtcgcat tggttgaaaa agccgagctc ggcggaatat gcctgaactg gggctgtatc
     1201 ccgaccaaag ccttgctcaa aaatgcggaa gtctggcaga atctgcgcac gttggacgaa
     1261 ttcgggatca caatagctca accgcaactc gatttcaccc aagttatccg ccgcagtcgt
     1321 gaggtcgccg ctcgcctttc gaaaggcatc gagtttctat ttaagaaaaa taaagtcgca
     1381 gtcgtccgag gttatggcga attgcgcgac cggcagacgg tcgcggtcat taatccggac
     1441 gataattcca ctactgggct caaggcgcga aatatcgtaa ttgctaccgg cgcccgcccg
     1501 cgctcgattc ctaatgtcga aattgacgga aaacgcatca tcagtagtcg cgaagctatg
     1561 attttgccgc agattccgaa atccattatc atcatcggtg caggcgccat tggagttgaa
     1621 ttcgcctacc tattcaacgc ctttggtgtc gaagtgaatc tggtcgaaat actgccacaa
     1681 attttaccga tcgaagatga ggaaatcacc aaagaactgt ctcgctcgtt tcgcaagagg
     1741 caaattggca ttcacattaa caccaaagtt aaaagtgttc aaacacaagg cgatttcgtc
     1801 gaagtggcgc tcaattccgg cggcgaatcg cagactctgg tcgccgacta tgctttagtg
     1861 gcaaccggcg ttcagccgaa ttcagataat ctgggtctcg cagaactcgg agttagcacc
     1921 gaaaaaggct ggattaaagt tgatgagcat tttcggacga acatcccgga cgtttacgcc
     1981 atcggcgatg tcattggtaa tccttgtctg gcgcatgttg cgtcggcaga gggcaagcac
     2041 gcggttgaat tcatagccgg gaagaatccc gcgccggtca attacgcggc gattcctagc
     2101 tgcgtttact gtcagccaca ggttgccagc gtcggcttaa cggagaaaag agcatcggaa
     2161 cttggctatg aattaaaaat cgggcgtttt atgttccgcg ctaacggcaa ggcggtggct
     2221 tccggcgaga gcgacggttt cgtcaaagtc attttcgatg gcaagacgca aaaattaatc
     2281 ggtgcccaca tcatcggcac cgaagcgact gaacttctac cagaactggt tctggcgatt
     2341 gatcgtgggt tgaattatag tgagataaag gattctattc atgcccatcc gactctgtcc
     2401 gaggcgctca ttgaagcggt ccgcgacgct ttcggcgaat cggttcacca gtaataatta
     2461 agttaattaa aaaacgggat agtcacgaac taacgtggtc ggaaattatc gaaaagaaaa
     2521 atactctggt gccctctgca gttaaaatac tagacgttct cgaccttggt ctgtgtccct
     2581 acggcgaagc ctgggaaatc cagaaaaaat tacatgccga acgccttgcc agtcaaattc
     2641 cggatacgct gattcttgtc gaacatccca atgtttatac tctggggaaa aatgccgatg
     2701 cccgacactt gctcgcctcg caagaatatt tgaaaaagcg cggcatagaa gtttatcaaa
     2761 ttgatcgggg cggcgacatt acctatcacg ggccgggaca attggtcggc tacccgattt
     2821 tcaatttaaa agagcataaa acgagcattg cttggtatgt caattctgtc gaagaagtat
     2881 tgattaaaac tctggtaaaa ttcgacatcc atgccgaacg aatcaaaggc ttgacgggaa
     2941 tctggttggc aaaccaaaaa attgcggcga ttgggatgcg cgtctcacac tgggttacga
     3001 tgcacggatt tgcgctgaat gtcacgacgg atttgtcgct attcggtgga attgtgcctt
     3061 gcggtctggc gacaaaaggc gttacccgaa tgtgcgattt gaattcggcg gttactatgg
     3121 atgctgtgaa ggcggtcatc ataaaagagt ttgtcgccca gtttgg
//
LOCUS       DAOMJU010000130         3160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_34879_length_3160_cov_18.658615, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000130 DAOMJU010000000
VERSION     DAOMJU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34879_length_3160_cov_18.658615"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLO58_09075"
     CDS             complement(<1..1097)
                     /locus_tag="PLO58_09075"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HQO74978.1"
                     /translation="MLEKTIKKPRLSFSLILLLSIISFFSFGHSQVQTILWPELNGTA
                     LWDSVIANFKPIKTLGYDKSRDTMYAVIDLKNGGSLSCVYTGYTIKLDRTKDPSTDAY
                     NKSIDCEHTWPQSMGADSEPQRSNLHHLYPTFTNVNSSRGNDPFAEIPDAKTDKWWYL
                     NKSQTIIPTSNIDKYSEKENDPPACFEPREDHKGNCARSMFYFYAMYSEAYFTKDPDM
                     TFWNFQKETLLAWNYYDTVDANEYARTWKIATYQDNKPNPFVLDSTLARRIWFYKSTT
                     LAKEKPNLTGDFKIHPCYPNPFNNTTNLAFTLPEIENVTLMVFDLNGHLVNEQQLGRF
                     PSGHHIVPWSGSNLSSGVYIYCLQAGQRAHAG"
     gene            complement(1099..3159)
                     /gene="fusA"
                     /locus_tag="PLO58_09080"
     CDS             complement(1099..3159)
                     /gene="fusA"
                     /locus_tag="PLO58_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQO74979.1"
                     /translation="MRNIGIVAHIDAGKTTTSERILFFTGMTHKIGEVHNGQAVMDFM
                     KQEQERGITITSAAITTEWKDHQINLIDTPGHIDFTVEVERSLRVLDGIVMVFCAVGG
                     VEPQSETVWHQADNYKVPRIAFINKMDRAGADYFQTINMMNEMLGANAVAFQLPIGDE
                     ADFNGIIDLITMKAYSYRELDMFESEIPSNLLPKAQKLREQMIEKLADFDENLMEKFI
                     NGMAISESDIRNAARYAVLNLCVTPVFCGSAFKNKGIRLLLDAIVDYLPSPVDRGIIT
                     GRDANNPEVIISRKPHISEPLTALAFKITNDAYVGQQTFVRVYSGELHSGSYIFNSTK
                     QKRERVGRVLRIRADERTEITSLKAGDIGALIGMKLTTTGDTLCNENDTILLENIRYP
                     ETVLDMKIEPLAIKERDRLGIALSRLALEDPSFKVRFADETEETIISGMGELHLEIIV
                     DRLKNEHKLDVNVGKPAVAFRETITREAEHTYRYKKQTGGKGQFAQIAFRLEPNPGKG
                     IEFVDRVKGGNIPREFIPAVEKGFREIVEKGLLAGYPMVDIKFVLFDGAYHPVDSSEM
                     AFRMCTILGMKEIINKTAPQLLEPIMKIEINTPDEYMGDAIGDINRRRGKIENMRRHR
                     KGSQKLNGTVPLMEMFGYASALRTISSGRAAFSMEFLHYTPLPKSIEESIIEERRKKK
                     AERD"
BASE COUNT          863 a          663 c          686 g          948 t
ORIGIN      
        1 ccagcatgcg cgcgttgccc agcctgaaga caataaatgt aaacgccact gctcagattg
       61 ctcccactcc aaggtacaat atgatgaccg gatggaaatc gtcctaactg ttgttcgtta
      121 actaaatggc catttaaatc gaataccatc agagtaacat tttcaatctc tggtaaggtg
      181 aatgccaaat tcgtggtgtt attaaaagga ttcggataac agggatggat tttgaagtcg
      241 ccggtcaagt ttggtttttc ttttgccaag gtggtcgatt tatagaacca gattctccgc
      301 gcaagggttg aatccagcac gaatggattg ggtttgttat cctgataagt ggcgattttc
      361 cacgtacgcg catattcgtt ggcatcaacg gtgtcgtaat aattccaggc taataaagtt
      421 tctttttgaa aattccaaaa agtcatatcg ggatctttag taaagtatgc ctccgaatac
      481 atggcataga aataaaacat cgaacgagca caattgcctt tatgatcttc acgcggttca
      541 aaacaggctg ggggatcgtt ttctttttcc gagtatttat caatattgga ggttggaatt
      601 atggtttgtg acttatttaa ataccaccat ttatcagttt tagcatctgg gatttcggca
      661 aaaggatcat tgccgcgtga agaattcacg ttggtaaagg tcggatacaa atggtgtaaa
      721 ttgcttctct gaggttcgga atcggcgccc attgattgtg gccaagtatg ttcgcaatca
      781 attgatttgt tataagcgtc ggtgcttggg tctttggtac ggtcaagttt gatcgtgtaa
      841 ccggtataga cacaggagag tgaaccacca ttttttaaat caatcacggc atacattgta
      901 tcgcgggatt tatcatagcc cagtgtttta atcggtttaa aattagcaat tactgaatcc
      961 catagtgctg tgccattcaa ttctggccat agaatcgttt gaacctgtga atgtccaaaa
     1021 gagaaaaagg aaatgattga caaaaggagt attaaggaaa aagatagtcg gggctttttt
     1081 atcgtttttt ctaacaaatt aatctctttc ggctttcttt ttgcgacgtt cttcaataat
     1141 actttcttca atactttttg gtaagggagt ataatgtaag aattccatcg aaaaagccgc
     1201 acggccgctg gaaatagtgc gtaaagccga ggcataaccg aacatttcca ttaatgggac
     1261 tgtgccattg agtttttgtg agcctttgcg gtgacggcgc atattttcaa ttttaccgcg
     1321 ccggcggttg atatcaccga tagcatcgcc catatattca tccggcgtat tgatttcgat
     1381 tttcataata ggttccaata attgcggtgc cgttttatta atgatttcct tcatccccag
     1441 gatcgtgcac attcggaaag ccatttcgct ggaatccacc gggtgataag cgccgtcgaa
     1501 aagaacgaat ttaatatcta ccatcgggta accggctaat aaaccttttt cgacgatctc
     1561 gcgaaaaccc ttttcgaccg ccgggatgaa ttcgcgcgga atattaccac ccttgacacg
     1621 atcgacaaac tcaataccct ttcctggatt tggctcgagc cggaaagcaa tctgagcaaa
     1681 ttgacctttg ccgccagttt gttttttata gcgataagta tgttcagctt cacgtgtaat
     1741 agtttcgcga aaagctaccg ccggtttacc gacattgacg tcgagtttgt gttcgttctt
     1801 taacctatct acgatgattt cgagatgtaa ttcgcccatt ccggagatga tagtttcttc
     1861 agtttcgtcg gcaaatcgca ctttaaatga ggggtcttca agagccaagc gactcaaggc
     1921 tattccaaga cgatctcgtt ctttgattgc gagtggttcg attttcatat ccagtacggt
     1981 ttcgggataa cgaatatttt cgagtaaaat agtatcattc tcgttacaaa gcgtatcacc
     2041 tgtagttgtc agtttcatac cgattaaagc gccaatatca ccggctttca aggatgtaat
     2101 ttctgtacgt tcatcggcgc gaatacgcag aacccgtccg acacgttccc gcttttgctt
     2161 ggtagaattg aagatataag aaccggaatg taattctccg gagtaaaccc gcacgaaagt
     2221 ctgttgaccc acgtaagcat cattagtgat tttgaatgcc aacgccgtca gcggttctga
     2281 aatatggggt ttacgtgaaa tgataacttc aggattatta gcatcacgtc cggtaattat
     2341 tccccgatca acaggggacg gcaggtaatc aactatggcg tcaagtagta agcgaatacc
     2401 tttatttttg aaagccgagc cacagaaaac cggtgttaca cataaattta agacggcata
     2461 acgagcggca ttacgaatgt cggactcact gattgccata ccattgataa acttctccat
     2521 caggttttcg tcgaaatcag ccaatttttc aatcatctgc tcgcgtaatt tttgggcttt
     2581 cggcaataaa ttagatggga tttcgctttc gaacatatca agttcgcgat atgaataggc
     2641 tttcattgta attaagtcaa tgattccatt aaaatcggct tcgtcgccaa ttggcaattg
     2701 gaaagcgacc gcattagcac ccaacatttc gttcatcata ttaatagtct gaaaataatc
     2761 ggctccggcg cgatccattt tattgataaa cgcgatgcgc ggaactttgt agttgtcagc
     2821 ctggtgccag acggtttcac tctgcggttc aacgccgcct actgcacaaa aaaccataac
     2881 tatcccatcc agaacacgca aagagcgttc gacttctact gtgaaatcga tatgaccggg
     2941 cgtatcgatg aggttgattt gatgatcttt ccattcggtg gtgatcgccg ccgaagtgat
     3001 cgtgatgccg cgttcttgtt cctgcttcat aaaatccata acagcttgac cattatggac
     3061 ttcgccgatt ttgtgagtca taccggtaaa aaagaggata cgctctgaag tcgtagtttt
     3121 accagcatct atgtgggcta caattccaat atttctaatt
//
LOCUS       DAOMJU010000131         3121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_35510_length_3121_cov_12.994781, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000131 DAOMJU010000000
VERSION     DAOMJU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35510_length_3121_cov_12.994781"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLO58_09085"
     CDS             <1..455
                     /locus_tag="PLO58_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74980.1"
                     /translation="QFIEMTGIYYFTNRTGQEIKLPIIYPFQVNSHQAFPDSIAVSLG
                     NGSPLHFEIRKESIYFSVPLLNKEQNALEIYFRQPITRPQFEYILTSTSSWQKPLEFA
                     DFMIRVPLTYRLANLSFPYEKIDTTDDFQVYHLHFENFYPEQNLIFSW"
     gene            512..2059
                     /locus_tag="PLO58_09090"
     CDS             512..2059
                     /locus_tag="PLO58_09090"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO74981.1"
                     /translation="MKKSPAILILFWATVGLANPIAAQLFSELYFDSTGWKIEVMGDW
                     YGENNERIVIVSLTDSAVTDTSFHLSGEFPVITPADMETDFYINPAGDYLKIGILNEY
                     GLYFFNNLIFGENGVIAAPRAGESIAFGGFIEASELMYYLDNTPTLGAINDCGNSKGT
                     IQGIVTDSSGMPIPDVKVKYSVTCSSESEDMSAVTDANGRFEFECLTAQQIFSFQHDH
                     YEFLKVPVQIWPDSTIEKTFVLTAFPPNYQNYFPLQDGNYWNYYSYIEDSYSEHISIN
                     ISATILINDKTYYLMGNDTIGYDSLKNVVIYKNQRDSLLYPLSLSDGNSMITPDSLWM
                     MKLNYDQTPITVRVGTFTDNIIVEKTFIGVRKSGLPPDTILTYFSKNVGIIKNINICH
                     SLISKSVERKELYHAYVNGTLYYENFYQVEQSFTPIKYNLSLTNYPNPFNSSTTIKYH
                     LAETGDVRLSVFDLSGRLVAELFKGRQPAGDYHYLWSGDNLPSGVYLLSLQAGGKRVI
                     QKCLLMK"
     gene            2236..2766
                     /locus_tag="PLO58_09095"
                     /pseudo
     CDS             2236..2766
                     /locus_tag="PLO58_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
     gene            2821..>3121
                     /locus_tag="PLO58_09100"
     CDS             2821..>3121
                     /locus_tag="PLO58_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQO74982.1"
                     /translation="MSRAREAFAQITENKTYPIPINGVLDLHTFSPKEIKDLIPEYLE
                     ACRAKDLLDVRIIHGKGIGVLRETVQAILRKLPYVEKFQLADESGGGWGATIVRLK"
BASE COUNT          908 a          645 c          638 g          930 t
ORIGIN      
        1 ctcaattcat tgagatgacc ggcatttact attttacaaa ccgcactgga caggaaatca
       61 aactgccgat tatctacccg tttcaggtca attctcatca agcttttccc gactcaattg
      121 cagtcagttt gggaaatggc agtcccttgc attttgagat tcgcaaggaa agtatctatt
      181 tttcagtacc tttattgaat aaggaacaaa acgcgcttga gatttacttc cggcaaccga
      241 ttactcggcc acaatttgag tatattctca cctcgaccag cagttggcag aaaccgttgg
      301 aatttgctga ttttatgatt cgggtaccgc ttacctatcg tctggcaaat ctgtcatttc
      361 cgtatgaaaa aattgacact accgacgatt ttcaggttta ccatttgcat tttgagaatt
      421 tctatccgga gcagaattta atttttagtt ggtaggttaa aagcacaaaa acacaaggac
      481 ttatgaaacc tttaataatg gagtaaaaac tatgaaaaaa agtcctgcga tattgatttt
      541 attttgggcg actgtgggat tagccaatcc aattgcagct caattatttt cggaattgta
      601 ttttgattct accggctgga agattgaagt tatgggtgac tggtatggcg aaaataacga
      661 acgtatcgtg atcgtgtctt taactgattc ggcagttacg gatacatctt tccatctttc
      721 aggtgaattt ccggttatta ctccagccga tatggaaaca gacttttata tcaatccggc
      781 tggtgattac ttgaaaattg gaatacttaa tgagtatgga ttgtatttct tcaacaattt
      841 aattttcggt gagaatggtg tcattgccgc tccacgtgcc ggagaatcta ttgcctttgg
      901 aggttttata gaagcttcag aattgatgta ctacttggat aataccccga ctctcggtgc
      961 tattaatgac tgcggaaatt caaaaggtac tattcaagga attgtcaccg acagtagcgg
     1021 catgccgatt cccgacgtaa aggtgaaata ttcagttact tgcagttccg aatctgaaga
     1081 tatgtcggca gttaccgacg cgaacggccg gttcgaattc gaatgcctga ctgcccaaca
     1141 gatatttagt ttccagcacg atcattatga atttctaaaa gttccggttc aaatctggcc
     1201 ggacagtacc attgaaaaaa catttgtctt aaccgccttt ccgcctaatt atcaaaatta
     1261 ttttccttta caagatggta attactggaa ttattatagt tatattgaag actcatacag
     1321 cgaacacatt tctattaaca tctccgcgac tatcttgatt aacgataaaa catactacct
     1381 aatgggaaat gatacgatag gttatgatag tcttaaaaat gtcgtgatct ataaaaatca
     1441 gcgtgattca cttttgtatc cactttcttt atcggatggc aattcaatga tcactcccga
     1501 ttctctctgg atgatgaaat taaattacga ccagactccg attaccgtta gagtcggaac
     1561 atttaccgac aatataatcg ttgaaaaaac gtttatagga gtaagaaaga gtggactacc
     1621 acccgatacc attctaactt atttctcgaa aaatgttggt attatcaaaa atattaatat
     1681 ttgtcactct cttatttcga aatccgttga gagaaaagaa ctatatcatg cctatgtaaa
     1741 cggcacctta tattatgaaa acttttacca agtcgaacaa tcttttactc cgataaaata
     1801 caacctgagt ttgaccaact atcctaatcc cttcaactcc tcaactacta ttaaatatca
     1861 tctcgccgaa accggcgacg tccggctgag cgttttcgac ctgagtggac gattggtagc
     1921 agagcttttt aaaggtcgtc aaccagctgg cgattatcat tatctctggt cgggtgacaa
     1981 tctgccgtcg ggtgtgtatc ttcttagtct gcaagcgggt ggtaagcgcg taattcagaa
     2041 atgtctacta atgaaataac aattctggct tcggaaagtt tactttacca taaaatataa
     2101 tttatataat ttagcgtgcc gtcctaatcg gcagattggt ttttgcaact tgttttagtt
     2161 ggtatggaac gataattttt aattatggaa aacatcatcc agatcgccgg tattcgaagt
     2221 atagatgaag ctcgaatgct actcgagagc ggtgcggatt gccttggttt cccattgcgg
     2281 ctggcttatc accaaccgga tctaaccgaa gtcgaagccg ccgaaatcat cgccaaactc
     2341 gcctgcggcg atagaagtgt cttaatcact tatctcgata atgcgcaaga aatcgcgagc
     2401 ctgagtcgtt tgctcaaagt aaaatatttg caactgcacg gtttcatctt acccaacgaa
     2461 gttcaaaagt tacgtcgcat aaatcccgat ctgagaatca tcaaaagttt aatcgtgcgt
     2521 tctggaaata tggatgaatt gcgctatact attgatcaat tcgccagtct ggttgaagct
     2581 tttatcacag atacatacga tcctaagacg ggcgcgaccg gcgccaccgg ccagacccat
     2641 gattgggaaa tcagccggaa aatcgtcgca tattcaccta aaccggttat tttagctggg
     2701 ggcttaaatc ccataaatat tcgtgaggca attttaaccg taaaaccggc cggagtggat
     2761 actcatactg gggtggagaa tcccgctgga tggaaggacg ctaaactcgt gcggcatttt
     2821 atctcaagag cgcgcgaggc gtttgctcaa attacagaga ataaaacata tcctattccg
     2881 atcaacggcg ttctcgatct acataccttc tcacctaaag aaatcaagga cttaattccc
     2941 gaatatcttg aggcgtgccg tgctaaagac ttgctcgacg ttcgtatcat tcacggcaaa
     3001 ggcattggcg tattgcggga gactgttcag gctattttac gcaaattgcc ttacgttgaa
     3061 aaatttcagt tagccgatga atccggtggc ggctggggtg cgacgattgt gcgcttaaaa
     3121 g
//
LOCUS       DAOMJU010000132         3002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_37630_length_3002_cov_20.173736, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000132 DAOMJU010000000
VERSION     DAOMJU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37630_length_3002_cov_20.173736"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1139)
                     /locus_tag="PLO58_09105"
     CDS             complement(3..1139)
                     /locus_tag="PLO58_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HQO74983.1"
                     /translation="MKRREFIGTLGITAATARISQAFPFISQLKTKTTDSGMKMRYEI
                     KTLKLKHTWTISRNSSDVKDNVFVYLEKDGITGIGEAAPNIRYDETPESTIELIKKAT
                     PLIERLNPSEFTQFGFELKEIESGQTAGKCAIDMALMDWIGKSLNLPLYKFWGLDPTK
                     APLTSFTIGIDTAEIVKQKTREAEPYKILKIKVGGPNDEEMITTVRSVAPDKPIRVDA
                     NEGWKDKEIAIRKIEWLAKNGIELVEQPLPSEMLEETRWIRERIEIPIIADEAVKTAS
                     DIPKLADAFDGINIKLMKAGGLQEALRMIWLAKSLGMKIMIGCMIESSVAISAATQLC
                     SLIDYADLDGNLLISNDPFSGAIFRDGRQFPADRPGLGVRKATG"
     gene            1317..2534
                     /locus_tag="PLO58_09110"
     CDS             1317..2534
                     /locus_tag="PLO58_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO74984.1"
                     /translation="MEEVRFKVYPYRWVVLIAFMVIIAVNQLCWITFAPITGRAAQFY
                     GVSDLSIGLLSMIFMIVYIVISIPASWIIDTYGIRIAVGIGAALTGVFGLMRGLLATS
                     YQYVLFAQIGLAIGQPFILNSITKVAARWFPIAERATASGLGSLAMYVGIIIGMALTP
                     YMTIRTGIEGTLLLYGVASVIGAVIFFVLVREHPPTPPCPAGQEERALVFDGLKQMLK
                     LKEFQRLMLIFFIGLGVFNGVTTWIENIVRPRGFSITQAGLTGGLMIVGGIIGAVIVP
                     MLSDRIGKRVPFIIQALVGATLGFIGVTFARSYWLLLTSAFVMGFFLLGVGPLGFQYG
                     AEISYPAPEGTSNGLLLLMGQISGVLFIFGMDGLKSPQTGSMTLPLVILIALMVLCTL
                     LSVNLKESNLLRK"
     gene            2671..>3002
                     /locus_tag="PLO58_09115"
     CDS             2671..>3002
                     /locus_tag="PLO58_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO74985.1"
                     /translation="MKKLVLSAVVVMFLMASLSYGFGLGIMLGEPSGLSGKYWMSEKN
                     AIAGGLAWSLSHDYMHVHADYLIHNNNLINIGGTILPFYYGLGAGIGFGDDFHLGARI
                     PLGIEYFFA"
BASE COUNT          725 a          582 c          727 g          968 t
ORIGIN      
        1 tctcagcctg ttgcttttct tacacctaaa cccggtctat ccgccgggaa ctggcggccg
       61 tccctgaaaa tcgcaccgct aaagggatcg ttcgagatta gcagattacc atcgaggtca
      121 gcgtaatcaa tcagcgaaca gagttgagtt gcggcggaaa tggcgaccga actttcgatc
      181 atacaaccga tcataatttt catgccaagt gatttagcca gccagatcat tcgcagggct
      241 tcctgcagac caccggcttt catcagtttg atgttaatgc catcgaaagc atcggcgagt
      301 ttagggatat cagaagcagt cttaacggct tcgtcggcga tgatcggaat ctcaatgcgc
      361 tcacgaatcc aacgagtttc ttccagcatc tcggatggca aaggttgttc aactagctcg
      421 atgccatttt tggctaacca ttcaatttta cgaatggcga tttccttatc tttccagcct
      481 tcattggcgt caacccggat tggtttatcc ggggcgacac tgcgcacggt agtaatcatc
      541 tcttcgtcgt ttggaccgcc gaccttgatt ttgagaattt tatatggttc agcttcgcgc
      601 gttttctgct tgacaatttc tgccgtatca atgccaatcg taaaggaggt caagggagct
      661 ttagtcggat cgagtcccca gaatttataa agcgggaggt taagagactt gccgatccag
      721 tccattaaag ccatatcaat ggcgcatttc ccggccgtct gtccggactc gatttctttc
      781 agctcgaagc caaattgggt aaattccgag ggatttagtc tttcgatcaa aggtgtggct
      841 tttttgatca gttcgatagt tgattctgga gtctcatcat agcgaatatt cggtgcggct
      901 tcgccgatac cggtgatgcc gtccttttca agataaacaa aaacattgtc tttcacatcg
      961 ctggaattgc gtgaaattgt ccaggtatgt ttcaatttca aagttttgat ttcgtaacgc
     1021 attttcatgc cagaatcggt ggttttggtt tttaattgag atataaaggg gaatgcctgt
     1081 gatatacgtg cggtcgcggc agtaatccct aaagtaccga taaattcacg tcgtttcatt
     1141 tcgatcctgt taaaatttaa atccgaatac gatgcaattt aatctcttta tataactgag
     1201 caaagttctt ttggtgtaaa taattctacc taaagcttgt gtcataattc taaatgtcat
     1261 aaaatcagtc gaatttatgt cgcaattaat ttccaattca tcggaggatt tgccagatgg
     1321 aagaagttcg atttaaagta tatccttatc gttgggtagt gctaattgca tttatggtta
     1381 tcatcgcggt gaatcagctc tgctggatca catttgcgcc aatcactggt agagccgcgc
     1441 aattctatgg ggtttctgac ctcagtatcg gtttattgtc tatgattttt atgatcgtgt
     1501 atattgttat ttcgattccg gcttcttgga ttattgacac gtacggaatt agaatcgccg
     1561 tcggaatcgg cgccgcctta actggagtat tcggtttgat gcgcggtttg ctggcgacga
     1621 gctatcaata tgtgctattt gcccagattg gactcgccat tggtcagcca ttcattttga
     1681 attccattac gaaagttgcg gcgcgttggt ttccgatcgc agagcgagca acggcttccg
     1741 gtttgggttc gctggcgatg tatgtcggta tcataatcgg aatggcattg acgccgtata
     1801 tgacaattcg aaccggaatt gagggcacat tattgctata tggagttgcc tcggttatcg
     1861 gagcggttat ttttttcgtg ctggttaggg aacatccgcc gacgccgccc tgtccggccg
     1921 gtcaggagga acgggctttg gttttcgatg gcttaaagca aatgctgaag ctaaaagaat
     1981 ttcagcgact gatgttgata ttttttattg ggctgggagt tttcaatgga gtgacaactt
     2041 ggatcgaaaa tattgtccga ccgcgtggtt tttcgattac acaagctggt cttaccggtg
     2101 gactaatgat tgtcggcggg ataatcgggg cggtaatcgt tccgatgcta tcggatcgga
     2161 tagggaaaag agtgccattt atcattcaag cgctagtggg agcaacactg ggatttatcg
     2221 gcgttacttt tgcgcggagt tattggctat tattgacttc ggctttcgtt atgggatttt
     2281 tcctgttggg agtaggtccg ttgggatttc aatacggggc tgaaatttcc tacccagctc
     2341 cggaaggaac ctcgaacggg ttattgttgc taatgggaca aatttctggg gtgcttttca
     2401 ttttcggaat ggacggtttg aaatcaccgc agaccggctc gatgaccctt ccactcgtaa
     2461 ttttaatcgc tctgatggtt ttatgtactt tgctcagcgt taatctgaaa gagtcgaatt
     2521 tgctccggaa ataaaaaaat gcatccgatt caaaaaccaa ctataaaaat tttaattttt
     2581 attgttattt taaggacaaa tattaattgc tattgtcatt gttgattttt atattttttg
     2641 cagtatttta aaattgagaa aggagtcatt atgaagaagt tagttttaag tgcagtagtt
     2701 gtgatgtttt taatggctag tttaagttat ggatttggtc tgggtattat gctgggtgaa
     2761 ccttccggtt tgagtggcaa atactggatg agtgaaaaga atgcgattgc tggtggatta
     2821 gcctggtcat tgagtcacga ttatatgcat gtgcatgcgg attaccttat tcataacaac
     2881 aatttgatta atattggtgg cactattctt ccattttatt atgggttggg cgcaggtatt
     2941 ggttttggcg atgattttca tctcggggca cggattccat tagggattga atatttcttt
     3001 gc
//
LOCUS       DAOMJU010000133         3001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_37651_length_3001_cov_23.946368, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000133 DAOMJU010000000
VERSION     DAOMJU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37651_length_3001_cov_23.946368"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PLO58_09120"
     CDS             <1..1047
                     /locus_tag="PLO58_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO74986.1"
                     /translation="VGTLTYWKRAHELNGNVCRYVTWYRSFGGYPEDICLNLPLIASK
                     PWFIKPRLAFIRHIDRRDPYAALPGYPPYWNPPNWQKKLFRMREILWQPIIDRAIREY
                     GLEDFDVYHFEWGADFYRDARFAKKMKSRGKKIICSYHGQDMRNRGVIPEMDKISDLN
                     LTSELDLTFRHPNLKYLFLPYDVLAFQPKNTPGKPLTICHATTNRRIKGSDEIIHVCE
                     ELERSHGIRFIFIENQPHSEVIELKKQADIYIDQITDHAWGYGMNSLEALSLGLVCVT
                     YLNPTYENFIPDHPFVNAGISNLKEKLLELIENPANLPARMAASREWVERRHDYRNVV
                     KELYQYYESIGVRF"
     gene            1049..2107
                     /locus_tag="PLO58_09125"
     CDS             1049..2107
                     /locus_tag="PLO58_09125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQO74987.1"
                     /translation="MKITFVLPGFIKIPMGGVKVVNEYANRLAEKGHEVTLVYPLALR
                     TGNRGYALRKKISSLIDRIQRVPESLYYTPNPIVNVMAVRNTTSKYIPNGDTIIAVGW
                     QTAEAVAELPAEHGRKFYLLQSFETYFSQKKRILATYHLPLEKIAVSHWIIDELEKLG
                     EKCFGPLGNAINPTEFFIENPQPERSNDVLMIYHPHKIKGAKEGLAALQLIKRQFPTL
                     TAIIVAPRKPVHRIPSWIKVIIRPSISELRHLYNISKIFLHPSRWEGWPLPPLEAMAC
                     GCAVVATANRGVQEYLVDRENALLCPIDDVKTLAQNVITLLESDQLREELAKGGIKTV
                     QRYNWRECTSRLEQYLMG"
     gene            2178..>3001
                     /locus_tag="PLO58_09130"
     CDS             2178..>3001
                     /locus_tag="PLO58_09130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQO74988.1"
                     /translation="MCLNSLLRQNYPQEKYSVIVVNDGSNDATGTLLRQMNLPENFGI
                     IEHPENCGLAAARNTGIQKSNAEIIIFLDSDMEVAPDFISRHIAWYKRPEVVGIVSEL
                     QPAPESPYDKYQRYLYESKRGARVIGANEPLPFQYFIFGITSVRWSALATVGGFNADI
                     RHYGGEDTELAYRLWQKYPTGLFFDPEIIVIHHHYRPFPQVLKIVEQFGRQVVPYIVE
                     KHPELSRLYGMQFVKPLGGNNWKLKYWIGRTLQTNVALEILWFLFEIKPYPISNFLV"
BASE COUNT          843 a          678 c          692 g          788 t
ORIGIN      
        1 gtcggcacgc ttacttactg gaaacgggca catgaattga acggcaatgt ctgccggtat
       61 gtaacctggt atcgcagttt cggcggttac ccggaagaca tttgtttgaa tctgccactg
      121 attgcgtcta aaccgtggtt tatcaaaccg cgactggctt tcattcgcca tatagatcgc
      181 cgcgatccgt atgccgcatt acccggctat ccaccttact ggaatccgcc gaactggcag
      241 aaaaagcttt tccgaatgcg cgaaatattg tggcaaccga ttattgaccg tgccattcgg
      301 gaatatggct tggaggattt cgatgtttac catttcgaat ggggcgcaga tttttaccgc
      361 gacgcgcgtt ttgctaaaaa aatgaagtca cgcggcaaaa aaatcatctg ctcctatcac
      421 ggtcaggata tgcgcaaccg cggcgtcatt ccggaaatgg ataaaatcag cgacttgaat
      481 ttaaccagcg aactggattt gacctttcgc catccgaatt tgaaatatct ttttttaccg
      541 tatgacgtgc tggcttttca gccgaaaaat acgcctggca aaccgctgac gatctgccat
      601 gcaaccacga atcgccgaat aaaaggctct gatgaaatca tccacgtctg cgaggaactc
      661 gaacggagtc acggtattcg ttttattttc atcgagaatc aaccacattc cgaagtgatc
      721 gagctgaaga aacaggctga tatttacatt gaccagatta ccgatcatgc ctggggctac
      781 ggtatgaatt cattggaggc actttcgctg ggattggttt gcgtgactta tttaaatccc
      841 acttacgaaa attttatacc cgatcatccg ttcgtgaatg ccggtatttc caacctgaaa
      901 gaaaaattac tggaactgat tgaaaatcca gccaatttgc cggctcggat ggcggctagc
      961 cgcgaatggg tggaaaggcg gcatgattat cggaatgtcg taaaggaatt gtatcagtat
     1021 tacgagtcca tcggagttcg attctaatat gaagataact tttgttttac ctggtttcat
     1081 taaaattccg atgggcgggg tcaaagtcgt caacgagtat gccaaccgcc tggctgaaaa
     1141 ggggcatgaa gtaactctgg tttatccgtt agctctgaga accggaaatc gaggctatgc
     1201 acttcgcaaa aaaatctcct cattaattga tcgaattcag cgcgtccccg aaagccttta
     1261 ttataccccg aatccgattg tgaacgttat ggcggtgcga aatactacat cgaaatatat
     1321 cccgaatggc gataccatta tagccgtcgg ttggcaaacg gcggaagccg tagcggagtt
     1381 accggcagaa cacggtcgca aattttattt attgcaaagt ttcgagacct atttttcgca
     1441 aaaaaagcgg attctggcaa cttatcatct gcctctggaa aaaattgcag tgtcgcactg
     1501 gatcattgac gagctggaaa agcttggtga aaaatgcttt ggtccgctcg gtaatgcaat
     1561 taatcccacc gaatttttca ttgaaaaccc tcaaccagaa cgtagtaacg acgtactgat
     1621 gatttatcat ccgcataaaa tcaaaggcgc caaagaaggt ttagcggctt tgcaattaat
     1681 caagagacaa ttcccgacat tgaccgcaat aatcgttgca ccccgtaagc cggttcatcg
     1741 aatcccttcc tggattaaag taattatccg gccgtccatc agcgagttga ggcatttata
     1801 caacataagt aaaatatttc ttcatccgag tcgctgggaa ggctggcctc taccgccttt
     1861 ggaagctatg gcttgcggtt gtgcggtagt ggcaactgcc aatcgcggtg tgcaggaata
     1921 tttagttgac cgggaaaacg ctcttctatg tccaattgat gatgtcaaaa ccttagccca
     1981 aaatgtaatc accctacttg aatcagatca actgcgagag gaactggcaa aaggtggtat
     2041 taagaccgtc cagcgttata attggagaga gtgcaccagc cgactcgaac aatatttaat
     2101 ggggtaatag cctgaataat tatcaaccaa ctgtcagcgt tatcgttccg gcttataatg
     2161 ccgctaaaac tctaccaatt tgcttaaatt cattgttacg gcaaaattac ccacaagaaa
     2221 aatattcggt tatagttgtt aatgatggtt caaatgatgc taccggaact ttacttcgac
     2281 aaatgaattt gccggaaaat tttggcatca ttgaacaccc agaaaactgc ggcttagccg
     2341 ccgcgcgcaa caccggaatc caaaaatcga acgctgaaat cataatcttt ctggactccg
     2401 atatggaagt agcgccggat ttcatcagtc gtcatattgc ctggtataaa agaccggagg
     2461 tcgtaggaat agtgagtgag ttgcaaccag cgccggaaag tccatacgac aaatatcagc
     2521 gctatctgta cgaaagtaag cggggagcac gcgttatcgg cgccaatgag ccactgccgt
     2581 ttcagtattt tatttttgga ataacctcag tcaggtggtc ggcactggcg actgtcggcg
     2641 gtttcaatgc tgatattcgc cactacggtg gcgaagacac cgagctggct taccgactct
     2701 ggcagaaata tccgacaggg ttgttcttcg atccggaaat tatagttatt caccaccatt
     2761 accggccgtt tccgcaggta ctaaaaatcg tcgaacaatt tggcagacag gttgtgcctt
     2821 atatcgtgga aaagcatccg gaactgagcc gattgtatgg catgcaattc gtcaaaccct
     2881 tgggcggtaa caactggaag ctaaaatact ggattgggcg aactctgcaa actaacgtgg
     2941 cgttagaaat tctctggttt ctatttgaaa taaagcccta tccaatttct aattttctgg
     3001 t
//
LOCUS       DAOMJU010000134         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_38669_length_2948_cov_21.030073, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000134 DAOMJU010000000
VERSION     DAOMJU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38669_length_2948_cov_21.030073"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PLO58_09135"
     CDS             complement(<1..1085)
                     /locus_tag="PLO58_09135"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HQO74989.1"
                     /translation="MAGMQDILKNSQESNNQNNSQKGKNEKNDNNFVGLSEMEAVKKA
                     ITENLGSTTNTFNSAADSTPVDFEPDLVITPADFPKNGFFKDSHLDEKKLKEIQCQDE
                     IAANQLYLDLLHELGNVFQSLKDNKPLDPQPLEKFIDLMHNSITSGDFLLLKAIQHKR
                     YAAWLISHSANVTILAYKIGLDLKYDRPTLHKLGISALLHDIGMLKVPNNIIFKHGQL
                     TPDEFEIIKKHPLDGFEMAKHLEQYPHIIEVILQEHEREDGSGYPQGLPGDEIAEFAK
                     IVGLADVFEAMVHGRAYREGFITYTAIQKIIEKRSKQFNPRIIRALINSVPMFPVGSL
                     VKLSTNEIARVLSVNSLRPVRPVVEIL"
     gene            complement(1091..1888)
                     /locus_tag="PLO58_09140"
     CDS             complement(1091..1888)
                     /locus_tag="PLO58_09140"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQO74990.1"
                     /translation="MYLDYWKIKEKPFENSPDPKFLFHSKQHDEALFRLLYAIQSSRG
                     AALLTGEYGCGKTLLMHTIISELSQGKFKIAYLTNPRWSAMELIEEILYQLEINSKSK
                     SRLEMLHSLNDFLFQNVQEKCQTIIIVDEAQVISDYETFEELRLLLNFQLNDRFLLTL
                     FLVGQPELNNMIKNIPQLNQRIAVRFHLKHFNFEETKAYIQYRLKVAGRNDQIFTDSA
                     FDEIYKYSEGIPRIINNICDLSLVIGFGKHLSTIDHNVIKGIINSER"
     gene            complement(1917..>2948)
                     /locus_tag="PLO58_09145"
     CDS             complement(1917..>2948)
                     /locus_tag="PLO58_09145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HQO74991.1"
                     /translation="SLMTFGLIPIFYKLGVIMDITDKPGGRHIHQHATPRTGGIAIFI
                     TFIITLNIVTKPPREFTGLFFASSVIAFVGIIDDIKHVPALIKLGAQILATIILIKYG
                     IYLSFLPNHIIGNIFSYVLTFIWIIGITNAYNCLDGLDGLAAGAAIIILLFYIIIATY
                     MVADEFMAIVGLILLGAIIGFMPYNFRITKNALIFLGDAGSTFIGFTLAALSIYGNWG
                     ISKSVDLVIPILLLGVPIADMIMTTITRIKDGKVKSVRQWLEYGGNDHFHHRLLQMGF
                     TKTGSVLIIWAITTVLGLLSLLLKQGDRLEAIIAILIALILFALIISLMLFEYRQERR
                     SLSNKKNIP"
BASE COUNT          876 a          559 c          612 g          901 t
ORIGIN      
        1 attagaattt ccactaccgg acgaaccggc cgcaagctgt tgaccgaaag cactctggca
       61 atttcgttgg tactaagttt gaccaaacta ccaacgggaa acattggtac actattgata
      121 agggcgcgaa taattctggg attgaattgt ttagaacgct tttcgattat tttctgaata
      181 gcagtgtaag tgatgaaacc ttcgcggtag gcgcgtccat gaaccatcgc ttcgaaaaca
      241 tccgccaaac ccacgatttt agcaaattct gctatttcgt cgcctggtaa gccttgcgga
      301 taaccggagc catcttcccg ttcgtgttcc tgtaaaatga cctcgataat atgcggatat
      361 tgttcaagat gcttagccat ttcgaatccg tccagcggat gtttcttgat gatctcaaat
      421 tcatctggcg ttagctgacc atgtttgaag ataatgttat taggtacctt gagcattcct
      481 atatcgtgta atagcgctga aattccgagc ttatgtaatg ttgggcggtc atattttaaa
      541 tctagtccaa ttttatacgc cagaattgtg acattggctg aatgggaaat aagccaggcg
      601 gcatagcgtt tgtgttgaat tgctttgagg agcaagaaat cgccggatgt gatggaatta
      661 tgcatcaaat cgataaattt ttctaacggt tgggggtcga gaggtttatt atcctttaga
      721 gattggaaaa catttcccaa ttcatgaagc aaatccaggt aaagctgatt ggctgcaatt
      781 tcatcctggc attggatttc tttcagtttc ttttcatcga ggtgactgtc tttgaagaaa
      841 ccatttttag ggaaatcagc cggagttatc accaaatccg gctcgaaatc aaccggcgtt
      901 gaatcggctg ctgaattaaa tgtattcgtt gtcgagccaa ggttttcggt aatggctttt
      961 tttacggctt ccatttcgct cagaccaacg aaattattat catttttttc gttttttccc
     1021 ttttgggaat tattttggtt atttgattcc tgactatttt tcaggatgtc ttgcattcct
     1081 gccatagata ttatctttct gaattaataa ttcctttgat gacattatgg tcaatagttg
     1141 agaggtgttt tccaaaaccg atcaccaagc ttaagtcaca tatgttattg ataattcgag
     1201 gaatgccctc tgagtactta taaatttcat caaatgctga atcggtaaat atctgatcat
     1261 tccggccagc aactttaaga cgatattgaa tgtaggcttt ggtttcctca aagttaaagt
     1321 gttttaaatg aaaacgaacc gctattcgct ggttaagctg gggtatattc tttatcatgt
     1381 tgtttaattc cggctgacca accagaaaaa gggtcagcag aaagcggtca ttaagttgga
     1441 agttcaataa taatcgcaat tcttcaaatg tttcataatc gctgattacc tgggcttcat
     1501 ctacgataat gattgtttga catttttctt ggacattttg gaacagaaag tcattgagac
     1561 tatgcagcat ttcgaggcgc gatttggatt tggaattaat ctccagctga taaagaattt
     1621 cctcaattaa ttccatagca ctccagcgcg gattggtcag ataagcaatt ttaaactttc
     1681 cctgcgagag ttcggagatg atcgtgtgca ttaacaaagt tttgccgcaa ccatattcac
     1741 cggtcaggag tgctgcccct cttgaactct ggatggcata tagtaaacga aatagagcct
     1801 catcgtgttg tttggaatga aataagaatt tcgggtccgg tgagttttcg aaaggctttt
     1861 cctttatttt ccaataatcc agatacatat atctaaatgt aagctaataa attcatttaa
     1921 ggaatatttt ttttatttga taaactgcga cgttcttggc gatattcaaa tagcataagt
     1981 gaaataatta gggcgaataa tattaaggca atcaggatag caatgatggc ttctaggcgg
     2041 tcaccttgtt tcaagagtaa cgacaacagt cccaatacgg tggtaattgc ccagattatt
     2101 aggacactac cagttttggt gaatcccatc tgtaagagtc ggtgatgaaa atgatcattg
     2161 ccaccgtatt caagccattg gcggacggat ttgactttgc catctttaat ccgagtaata
     2221 gtagtcatta tcatatcggc aatcggtaca ccgaggagga gaattggaat caccagatca
     2281 acgctcttac taatccccca atttccataa atactcaaag ccgctaatgt aaatccgata
     2341 aaggtgcttc cggcatctcc taagaaaatt aaggcgtttt ttgtaattct gaaattatat
     2401 ggcatgaatc caataattgc acccaataat atcaacccaa ctatagccat aaattcatcg
     2461 gcaaccatat aagtagcgat gataatgtaa aaaagcaaga ttattatagc agctccagcg
     2521 gctaagccgt ctaatccgtc gaggcagtta taggcgttag tgatgccgat aatccagata
     2581 aaagtcaata cataggaaaa aatattgcca attatatgat taggaaggaa ggacaaataa
     2641 atcccatatt tgattaaaat tatcgtagcc agaatctggg caccaagttt tatcagtgcg
     2701 ggaacgtgtt taatatcgtc aataataccg acaaaagcta ttacactact ggcgaaaaaa
     2761 agcccggtaa actcgcgcgg cggtttggtg acaatattaa gcgtaatgat aaaggtaata
     2821 aagatggcaa tgccaccagt ccgaggggtg gcatgctgat gaatgtgccg accacccggt
     2881 ttatcggtta tatccataat tacaccgagc ttgtagaaaa tgggtattag accgaaagtc
     2941 atcaaact
//
LOCUS       DAOMJU010000135         2900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_39606_length_2900_cov_20.372583, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000135 DAOMJU010000000
VERSION     DAOMJU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39606_length_2900_cov_20.372583"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..228
                     /locus_tag="PLO58_09150"
     CDS             13..228
                     /locus_tag="PLO58_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQO74992.1"
                     /translation="MPIYEFECQNCGRVFEELVFLKNFSESEITCPHCGFKQAKKLIS
                     APALSRSSTCSSCGAKSCSSSCGSRSH"
     gene            complement(393..1217)
                     /gene="proC"
                     /locus_tag="PLO58_09155"
     CDS             complement(393..1217)
                     /gene="proC"
                     /locus_tag="PLO58_09155"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQO74993.1"
                     /translation="MKNNQIAIIGAGNIGTAIANGLADSNRFKPAEIILTRRRIHLLD
                     DMQKRGFIIEKDNCQAVRQAKIIIIAVEPQQVNSVLTEIAPQLKPNHHIVISVVTGVN
                     TSQIVKKVGSGIAVVRAMPNTAIAVRESMTCLASDTASEAEIAITKSIFDTVGKTIVI
                     EEEKMTAATALGACGIAFFLRAIRAASQGGIEIGFHSQEAIDIAAQTARGAATLILTH
                     ANHPEYEIDRVTTPRGCTIAGLNQMEHEGFSSAMIKGITVSAEHAKSLYKNTNNSD"
     assembly_gap    1303..1402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1403..2797)
                     /locus_tag="PLO58_09160"
     CDS             complement(1403..2797)
                     /locus_tag="PLO58_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQO74994.1"
                     /translation="MTGNHARKIEYLKKKASRISIREGAAYGLFDGFGLRYITPYALL
                     LGMSNTLIGILISLPQMLGTLIQLLTLKFLEKGIHRRTIAWVGTSLQTLMWFPIIFLG
                     WLHFNYNLNNMVISAGLMVFYSLMVIFGGIVGPAWSSWMGDIIPRQIGTYFGLRNRVV
                     GFTTILAMLSAGLILQHFNRLGQPFTGFAIIFLIAFTGRALSSALLKRQYEPEFHPQK
                     EYYFTIWQFIRKIPQSNFGKFVTYVALMQFAVYISSPFFAVFILEDLHFNYFQFTLIT
                     MTMPFVNMLLMPAWGRFADRFGNLRVVRLTGFCLPIIPALWVLAVFFIDRPVLAVLYL
                     ILVETLSGFAWAGFNLTTVNFIYDAVTRQRMAICVAYYNIFNGIGMFLGGLIGGLIAD
                     LPFQFHRFSALALVLMLSALIRLLVSAVMLPLIREVREVDHFDIKEARKRLAMLSPVD
                     ILRVLSLKLGNPRH"
BASE COUNT          877 a          614 c          600 g          709 t
ORIGIN      
        1 attttagcta aaatgccaat ttacgagttt gaatgtcaga attgtgggag ggttttcgag
       61 gaattagtct tcttaaaaaa cttttcagaa agcgaaatta catgtccgca ttgcggattt
      121 aagcaagcga aaaaattgat ctcggcaccg gcgctaagta gatcatccac ttgctcttct
      181 tgcggcgcta aatcctgttc cagtagttgt ggttctcgaa gccattgatt cattacggca
      241 attgccggaa ctcatctttt gacgaaccaa taaaataaaa tttatagata aaatcgagga
      301 tttattcagc ttgaaatgaa aagtagcaaa ttgagtgata aattgtcttt agcagtttaa
      361 tttccccgta taagcattaa aaactaactc agttaatcac tattatttgt atttttatat
      421 agacttttag cgtgctcggc tgaaacagta atacctttta tcattgctga actaaaaccc
      481 tcgtgttcca tctgattcaa gccggcaatc gtacagccac gaggagtcgt gacccggtca
      541 atttcatatt cgggatggtt ggcatgagtt aggatcaaag tcgccgctcc gcgggcagtc
      601 tgggcggcaa tatcaattgc ttcctgggag tgaaaaccaa tctcgatgcc gccttgagaa
      661 gccgcccgaa ttgctcgtag gaaaaaggcg attccacaag ctcccaaagc agttgcggca
      721 gtcatttttt cctcttcgat gactatagtt ttgcccaccg tatcgaaaat agatttggta
      781 attgcgattt cagcttccga agcggtatct gatgccagac aggtcattga ttcccgtacg
      841 gctattgcag tattgggcat tgcgcgcacg accgcgatgc cagatccgac ctttttaaca
      901 atttgagaag tattgactcc cgtcacgact gatatgacga tatggtggtt gggttttaat
      961 tggggcgcaa tttcagtaag aaccgaattg acctgctgtg gttcaacggc aatgataata
     1021 atttttgcct gacgaacggc ctggcagtta tctttttcga taataaaacc acgtttttgc
     1081 atatcatcca gcaagtgaat gcgacgtcga gttaaaataa tctccgcagg cttgaaacgg
     1141 ttagagtcgg ccaaaccatt ggctattgca gtcccaatat ttccagcacc aatgattgca
     1201 atttgattgt ttttcatagt gaattctcct ttagcttcaa acggatgccc ctgaccagtc
     1261 gtatatttca cggttaatcc gccgatggat aaacgctgtc tgnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnctaatgac gcggattgcc gagttttagc gagagaactc
     1441 ggagaatatc caccggtgat aacattgcca gccgcttgcg cgcctcttta atatcaaaat
     1501 gatctacttc gcgcacttca cgaatcaacg gtaacataac agctgagaca agcagacgga
     1561 tcaaagcact gagcattaaa actagtgcta acgccgaaaa gcggtgaaat tggaacggta
     1621 agtcagcaat caacccgccg atcaaaccac cgagaaacat cccaatgcca ttaaaaatgt
     1681 tgtagtaagc cacacaaatc gccatccgct ggcgagtaac cgcatcgtaa atgaaattga
     1741 cggttgtgag attgaatcca gcccaggcaa atccactcag ggtttcgact aagatcaagt
     1801 acagcaccgc taaaacagga cgatcaataa aaaatactgc caaaacccat aatgccggaa
     1861 tgatcgggag gcaaaaaccg gtgaggcgca cgacccgcaa attgccgaag cgatcggcaa
     1921 agcgtcccca agcgggcatt agaagcatat tgacgaacgg catagtcata gtaataagag
     1981 tgaactgaaa ataattgaag tgcaaatcct ccagtataaa tactgcaaaa aacggcgaag
     2041 aaatatatac ggcaaattgc atcagggcaa cgtaagtaac aaatttaccg aaattgctct
     2101 ggggaatctt gcgaatgaac tgccaaatcg tgaagtaata ttccttctga ggatgaaatt
     2161 ccggttcgta ttgacgtttt aacaaagctg acgaaagagc gcgtccggta aaagcaatca
     2221 gaaaaatgat cgcaaagccc gtaaagggtt gccccaacct gttgaaatgt tgcaggatta
     2281 agccagcact aagcatagcg agaatagtgg taaatcctac aacccgattg cgaaggccaa
     2341 aataagtccc gatttggcgc gggatgatgt cgcccatcca ggaactccag gctgggccaa
     2401 caataccgcc gaaaattacc atcagactat aaaaaaccat tagtccagca gaaatcacca
     2461 tattgtttag attataatta aaatgcaacc aacccaggaa aataatcggg aaccacatta
     2521 aggtttgcaa agaagtaccc acccaagcaa tggttctacg gtgaatacct ttctctaaaa
     2581 atttcaacgt caagagttgg atcaaggtcc ccagcatctg tggtaaagaa atcaaaattc
     2641 caattagagt attgctcatt ccgagcaaaa gggcatacgg agtaatgtaa cgcaagccaa
     2701 atccgtcgaa caaaccatac gcggcgccct cacggatgct gatccgactg gcttttttct
     2761 taagatattc aattttacga gcgtggtttc cagtcatcgg gcgcaaattt attgagtagc
     2821 caaattgtat ccaagtcact tgtaaacaat aaaaatttat acgtcatatc cctaaaatgg
     2881 tcgatcgata gacagatttt
//
LOCUS       DAOMJU010000136         2589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_46677_length_2589_cov_21.170481, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000136 DAOMJU010000000
VERSION     DAOMJU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46677_length_2589_cov_21.170481"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLO58_09165"
     CDS             complement(<1..591)
                     /locus_tag="PLO58_09165"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HQO74995.1"
                     /translation="MSGFEMCCRALHGEELTPEQLKHLCDRDWIRTNARYIYKDKVNI
                     LAEIELEINGVPQAVVIKWFGWRNRISYWLSPFMRSRAKKSWDASFSLLKCGVKVPKP
                     LTVYTQRHFGFIKCNFLLTEKIENFTLARNWLRDPHAKPSDKRIIVETLAEIVRKMHQ
                     AGMVHSDLTPGNFLINNNNPSEIYLIDLNRLKHKIFL"
     gene            complement(578..1612)
                     /locus_tag="PLO58_09170"
     CDS             complement(578..1612)
                     /locus_tag="PLO58_09170"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HQO74996.1"
                     /translation="MRYLFFITKPYSISVIRPLYEEIFRGAWGEALIFATPPVKALID
                     FAAPSTDSIKAAIDFQPEIVFVPGNFVHDKIPGLKVQIFHGLCEEKGGHYKITGFFDL
                     YCTSGPLITEKFLQLSKKYGFFKVKETGWPKIDNLLKPYNRNEICQSLQIDPQSKIIL
                     YAPTFSPKFKSSNEILKVLNDIPRSGEQWIIKFHDLMASEDRRCFEKLPQDKFRIYTG
                     YDNTPLLQVADVLISDTSSIVYEFMLLDKPVVTIGAKVRQEKALNIESASDLRAAVER
                     SLENPQEFAENRRRTLAQIHPYIDTLNSQRVLQAVEEFLSGMAPGELKKKPLNLYRKY
                     KVRRQLGVWF"
     gene            complement(1615..>2589)
                     /locus_tag="PLO58_09175"
     CDS             complement(1615..>2589)
                     /locus_tag="PLO58_09175"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HQO74997.1"
                     /translation="DKSRNDYEWSRKIIGDLGGNFITADNEKERVEKIIKQNFDVVVF
                     GKSRGAEKFCRPSSLAVLLYHGIGVKACYYTDYNPRIDVRYVESQYRLEELRRRNIKT
                     ELVVTGFPKLDPLIDSSVRDYYSDKYHLAKSKPTILYAPTFYPSSIEVFGESLAEQTK
                     DFNLIVKLHHFSWLMKKYQHQRDLWQTLMQKYEHVRLAPVEEYNIVPLFSCSDILVTE
                     ASSTAFEYLATSRPIIIADFVKLRWKHRLFRKNFERQRLDSAIIDELDFAYHLEKKQD
                     LHDVLNRAIHDVEGKRHLLTRKRDQFLGNVDGKASERVVADLKNRLNK"
BASE COUNT          729 a          585 c          509 g          766 t
ORIGIN      
        1 cagaaatatt ttatgtttta gacggttaag atcaattaga taaatttcag acggattatt
       61 gttattgatg agaaaattcc ctggcgtcaa atctgaatgc accataccgg cttggtgcat
      121 tttcctgact atttcagcaa gagtttcaac gataattctt ttatcggacg gcttagcgtg
      181 tgggtctctt aaccaattac gcgccagggt aaaattctct attttctcgg ttaataaaaa
      241 attgcatttg atgaatccaa aatgacgctg agtataaacc gtaagcggct tgggaacttt
      301 gaccccacat ttcagcaaac taaatgaagc gtcccaggat ttcttggcac gtgaacgcat
      361 aaagggactt aaccagtagg aaatccggtt gcgccagcca aaccacttaa tcacgactgc
      421 ctgcgggact ccgtttattt ccaattcgat ttccgctagg atattgacct tatctttgta
      481 aatataacga gcattagtac gaatccaatc gcggtcgcac aaatgcttca gctgttctgg
      541 agtaagctct tctccatgta acgctcgaca acacatttca aaaccagaca cctaactgtc
      601 tgcgcacttt atatttacga taaagattta acggcttttt cttcaattct ccgggagcca
      661 tgccgctcaa aaattcttct actgcttgta gaacgcgttg actatttaat gtatcaatgt
      721 aaggatgaat ctgcgccagc gttcggcgcc gattttcagc gaattcctgc ggattttcca
      781 gtgaacgctc gaccgccgcc cgcaaatctg acgctgattc aatgttaagt gctttttctt
      841 gcctgacttt cgcgccgatt gttaccaccg gtttatccaa cagcataaat tcatacacta
      901 ttgacgaagt atccgatatc aaaacatcgg cgacttgcaa taacggagta ttatcgtagc
      961 cagtatagat ccggaattta tcctggggca atttttcaaa acaccggcgg tcttcggaag
     1021 ccattagatc gtgaaactta atgatccact gttcgccaga gcggggtata tcattgagaa
     1081 cttttagtat ttcgtttgag gatttgaatt tcgggctaaa agtcggagcg tatagaatta
     1141 ttttgctctg cgggtcaatc tgtagacttt gacaaatttc atttcgatta tatggcttca
     1201 gtagattgtc aattttcggc cagccagttt cctttacttt aaaaaaacca tacttttttg
     1261 acaattgcaa aaatttttca gtaatcaagg gaccgctggt acagtagaga tcgaagaaac
     1321 cagtaatttt ataatgtcca cccttttcct cacacagtcc gtgaaaaatt tgcaccttta
     1381 aaccgggaat tttatcatga acgaaattgc ccggcacgaa aacaatttcc ggctggaaat
     1441 ctatcgccgc tttaatcgaa tcagttgaag gcgccgcaaa atcaatcagg gctttgactg
     1501 gcggtgtagc aaatattaaa gcctcccccc acgctccgcg gaaaatttcc tcatacaatg
     1561 gccggataac gctaattgaa tacggcttgg taatgaaaaa taaatagcgc atttttactt
     1621 attcagacga tttttcaaat cagcgactac tcgctcggat gccttgccat caacatttcc
     1681 cagaaactga tcccgtttcc gggtcagcaa atgtctcttc ccctcaacgt catgtattgc
     1741 acgattcagt acatcgtgca aatcttgctt cttttcgagg tgataagcaa aatcgagttc
     1801 gtcaattatc gccgagtcaa gacgttgccg ttcgaaattt tttcgaaaaa ggcggtgctt
     1861 ccagcgcaat ttaacaaaat cagcgatgat tatcgggcgg ctggtagcga gatattcaaa
     1921 agccgttgac gacgcttctg ttactaaaat atcactgcac gaaaaaagcg gcactatatt
     1981 gtattcctcg accggggcta aacggacatg ttcatacttt tgcattaggg tttgccagag
     2041 gtcgcgttga tgttgatatt ttttcattaa ccaggaaaaa tgatgcaatt tgacgatgag
     2101 gttaaaatcc ttcgtctgct ctgccagcga ttcgccaaaa acttcaatcg aactcggata
     2161 aaaggtcggg gcatataaaa tcgtcggttt gcttttcgcc aaatgatact tgtcggaata
     2221 ataatctcta acagaactgt ctatcagtgg gtccaatttg gggaaaccgg ttacgaccaa
     2281 ctcagtcttg atattccgtc gccgcaattc ttccaggcga tattgactct cgacatagcg
     2341 cacatctatc cgtggattgt agtcagtata ataacaggct tttaccccta ttccgtgata
     2401 tagcaaaact gccagggatg aaggccggca aaacttttcc gcaccgcggc ttttgccgaa
     2461 aaccacaaca tcgaaatttt gcttaattat cttttcgaca cgctcttttt cattatcagc
     2521 tgtaataaaa tttccgccca aatcaccgat aatttttcgc gaccattcat aatcattacg
     2581 acttttatc
//
LOCUS       DAOMJU010000137         2484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_49615_length_2484_cov_17.037464, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000137 DAOMJU010000000
VERSION     DAOMJU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49615_length_2484_cov_17.037464"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2305
                     /locus_tag="PLO58_09180"
     CDS             <1..2305
                     /locus_tag="PLO58_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQO74998.1"
                     /translation="RIMESAQAILWHSPVVRQEGTEPEFLWKTRYFNLDAIKNIIPLP
                     DYPTHDINDRYYYSILEEDRQAMDQVSSAALKSGADRYSQEFRLRDANGVIHWMYEDA
                     RIKKIDESHFEVVGVITEVTERKKAETALKLSEERFKSLFEYNSSAVIIYEVVDNGND
                     FIIRDLNKASEKIEKVKRSEVVGKNILEAFQGVKDFGLFEVLQRVWRTGEPENFPVTF
                     YKDNRISGWRDNYVFKLPSGEVVAVYNDLTKQKQDEEERRKSEENFRLVWENSVDGMR
                     LTDENGIVIMVNKAYCNLVDLPREKLEGQQFTLIYAKEMQAKNMEHYLQRFHGKTDRN
                     YFEMEVLLHNGRKVWFSLSDSYFETAPGKRLLLTIIRDITEQKMRQIEKEELQSQIVQ
                     LQKMEAVGNLAGGVAHDFNNLLTIIQGYTQMLMAQKDKTEPDYQGLKHVLDACDKAAN
                     LTRQLLLFSRKQPMEFKPVNINNTITNLLKMIKRLIGENIKIITNLKEPIQNIEADEG
                     NIEQVIINLVVNARDAMPNGGTLTISTDNVALSAKECQTIKDSHPGRFVRVSVADTGQ
                     GIPSDIIEKIFEPFFTTKEAGKGTGLGLSVVYGIIKKHNGWINVQSEIGAGTVFDIYL
                     PATEITVDDKAHLSAETQMKQGNGEKILTIEDSADVLKFLAETLHQNGYTPITAANAA
                     EAIEQFQNENGQIDLILADVVLPDKNGVTVSEEILAQKEDIPVILYSGYAEESIIQLI
                     KKNPKFVFIQKPFKMKELLDLIYQKLH"
BASE COUNT          805 a          420 c          580 g          679 t
ORIGIN      
        1 ccggataatg gaaagcgctc aggctattct ctggcattca ccggtagtca gacaggaggg
       61 aactgaaccg gaatttctat ggaaaactcg ctatttcaat ctggacgcca ttaaaaatat
      121 tatcccgctc cctgattatc caactcatga tattaatgat cgttattatt acagtatttt
      181 agaagaagac cgccaggcaa tggatcaagt cagttctgct gcacttaaga gcggtgccga
      241 tcgctatagc caggaatttc gcttgcgaga cgcaaatggt gtaattcact ggatgtatga
      301 agacgcgcga atcaagaaaa ttgatgaatc gcattttgag gtagttggtg tcattactga
      361 ggttacggaa cgcaagaaag ccgagacagc cttaaaatta agtgaagaaa gattcaagtc
      421 attatttgaa tataatagta gcgccgtaat tatatatgaa gtggttgata atggtaatga
      481 tttcattatc agagatttaa ataaagccag tgaaaaaatt gagaaggtaa aaagaagtga
      541 ggtagtcggt aagaatatct tggaagcatt tcagggagta aaagattttg gtttatttga
      601 agttttacaa cgtgtctggc ggacgggtga gccggagaat tttccagtga cattttataa
      661 ggataatcgc atctccggct ggagagataa ttatgttttt aaactgccga gcggcgaagt
      721 tgtagcggtt tataatgacc taactaaaca aaagcaggat gaagaagaga ggcgcaagtc
      781 tgaggagaat tttcggttgg tatgggaaaa ttccgtagat ggtatgcgcc tgaccgatga
      841 aaatggtata gtcataatgg tcaacaaggc ttattgcaat ctggttgatc ttcccagaga
      901 aaagctagag ggacaacaat ttaccttgat ctatgctaaa gagatgcaag ctaaaaatat
      961 ggagcactat cttcagcgtt ttcatggcaa aaccgaccga aattattttg aaatggaagt
     1021 attgcttcat aatgggagaa aagtctggtt tagtctatcg gattcctatt ttgagactgc
     1081 gcccggcaaa cgtttattat tgacgattat tcgtgatatt actgaacaaa aaatgcggca
     1141 gatcgaaaaa gaagagttac aaagtcagat tgttcagctc cagaaaatgg aagcggtcgg
     1201 taatctggca ggtggagtcg ctcatgattt caataatttg ttgacaatta ttcagggata
     1261 tacccaaatg ctaatggcgc aaaaggacaa gactgaacca gattatcagg gattaaaaca
     1321 tgtattggat gcctgtgata aagcggctaa tttgaccagg caattattgc tattcagccg
     1381 gaaacaacct atggaattca aaccggtcaa tatcaacaac actataacta atctattaaa
     1441 aatgatcaaa cgccttattg gtgaaaatat taaaattatc actaatctaa aagagcccat
     1501 ccagaatatc gaagccgatg aaggtaatat tgagcaggtt atcattaatt tggtagtaaa
     1561 tgcgcgtgac gccatgccga acggcggtac cttaactatt tcaactgaca atgttgctct
     1621 ttcagccaaa gagtgccaga caatcaagga ttcccacccc ggccggtttg tgcgggtgtc
     1681 agttgccgat accggtcagg ggattccgtc tgatataatc gaaaaaatat tcgagccgtt
     1741 tttcactacg aaagaagccg gaaaaggaac tggtctcgga ttatcggtag tatatggcat
     1801 cattaagaaa cataacggtt ggatcaatgt tcagagcgaa attggtgccg gaacggtttt
     1861 tgacatttat ctgccagcaa ctgagataac ggtggatgat aaagctcatc tatcggctga
     1921 aactcagatg aaacaaggga atggagaaaa gattttgact atcgaggata gtgccgacgt
     1981 tttgaaattt ctagcggaaa ctcttcacca aaatggatat actccgatta cggcggctaa
     2041 tgccgctgag gctattgagc aattccagaa cgaaaatgga caaattgatt tgatattggc
     2101 agatgttgta ttgccggaca aaaatggagt aactgtatct gaagaaatat tagcccaaaa
     2161 agaagatatt ccggttatac tctatagtgg ctatgctgaa gaatctatca tacaattgat
     2221 caagaaaaat cccaaatttg tttttatcca gaagcctttt aaaatgaagg aattattgga
     2281 tttgatatat caaaaattgc attgattatt gagtgtagga caggcattct tgcctgtcaa
     2341 ttttaatggt gggtaatatc taactgacag caccaagctt atgctcgcgg tggttggcga
     2401 atacggtgcc tgtcaatttt aatggtgggt aatgtctaac tgacagcacc aagcttatgc
     2461 tcgcggtggt tggcgaatac ggtg
//
LOCUS       DAOMJU010000138         2460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_50368_length_2460_cov_15.311850, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000138 DAOMJU010000000
VERSION     DAOMJU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50368_length_2460_cov_15.311850"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PLO58_09185"
     CDS             <1..1119
                     /locus_tag="PLO58_09185"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HQO74999.1"
                     /translation="SMPYKTVFDAYHLYHLPQFDPVIDLLLNDNRFEVFLTTSSANLP
                     DEQNLTKHIFQRRNVQCIFANNEEERAAKIRSLNPDVFICGWSRYPIEKFVTEKTLVG
                     MIYHGIGVKPSYWRDNHPRLNIRFVEGPFRMRQLREKGIQTDLELTGFPKIDPLFNGQ
                     IASREEILRNLGLDFNKKTLLYAPTFYPSSFESFGMKLPEMAKDLNLIIKLHQWSYFL
                     EKFSGVNLRRHIKLANKIRKKYPRVVMIEPEHYNIIDLYQASDILLTEASSTIYEFMA
                     LRKHIIICDFFKKKIEHHLLPKRIYLHRLDPEMHGIMTEFCYHIKQPRDLPVALNKCF
                     QQPDPFVELREKYIADMLYKLDGRASERIRNSILSRVN"
     gene            1151..1879
                     /locus_tag="PLO58_09190"
     CDS             1151..1879
                     /locus_tag="PLO58_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HQO75000.1"
                     /translation="MKVIILAAGNAIRLASLANGSPKCLLPLGNTTILGKQLHNLEKV
                     GLTDVILSVGYKVEKVEEYLKNFRTNLKIHLEFNPFYDISNNIVSLWNVRHLVWNQPI
                     IIVNGDNVFDYQILSLLLESPFENVLMIQEKDEYDGDDMKVKTENDRLLAVNKTMKSS
                     EANGESIGIMKFSAEGCAQLMQKIVEMTRNKENLQVWYLRSIQELINDGLPVHVCRIN
                     GLKWEEVDFPEDYLRVKNMNWEGN"
     gene            1892..>2460
                     /locus_tag="PLO58_09195"
     CDS             1892..>2460
                     /locus_tag="PLO58_09195"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HQO75001.1"
                     /translation="MSGKIKLDDKYLEKSLRLLERATTLLEKYNIPYWLEGGTLLGII
                     RENRLLPWDNDLDISIRSEDFDRLRRILPKFYYRGMIAKVREHKIDDPPFQKGEVRLI
                     KVYATKYLFFKTPLVLDIFVKKKLDDQYYWVVGVKRRAKKAVPARFYDELTTVQFNNK
                     TYSIPKLTDEYLTYRYGDWRTPVKTWNYI"
BASE COUNT          788 a          441 c          501 g          730 t
ORIGIN      
        1 tctatgccat ataaaactgt tttcgatgcc taccatttat atcatctccc gcaattcgat
       61 ccggttattg acctcttgtt aaatgataat cgctttgagg tttttctgac gacctcaagt
      121 gctaatttgc cagatgaaca gaacctgaca aaacatatat ttcagcggcg caatgtgcag
      181 tgcattttcg ctaataatga agaagaacgc gccgctaaga ttcgatctct taatccggat
      241 gtattcatct gtggttggtc gcgttatcca atcgagaaat tcgtcacaga aaaaactctg
      301 gtcggtatga tctatcacgg tattggagtt aaaccatcct attggcgaga taaccatcca
      361 cgattgaata tccgctttgt cgaggggccc tttcgtatgc gacaactgcg tgaaaaagga
      421 atacagactg atttggaact gacaggcttt cccaaaattg atccgctgtt caatggccag
      481 attgccagcc gggaggaaat tttacgtaat cttgggctgg actttaataa gaaaaccctg
      541 ttatatgctc cgacttttta tcccagtagt tttgaatcat ttgggatgaa attgccagaa
      601 atggctaaag atttgaactt gataatcaaa ttgcatcaat ggtcttattt tttggaaaaa
      661 tttagtgggg tcaatctacg ccgtcatatt aaactagcta acaaaatcag gaagaaatat
      721 ccccgagtgg tgatgatcga gccagaacac tataatatta ttgaccttta tcaggcatct
      781 gatattttat taacggaagc ctcttcgact atttatgagt ttatggcttt acgcaaacat
      841 ataatcattt gtgatttttt caagaaaaag attgaacacc atctcttacc aaaacgaatt
      901 tatttgcatc gtcttgatcc cgaaatgcat ggaattatga ccgaattttg ctatcatatc
      961 aaacaaccgc gagatttacc tgtagctctg aataaatgtt tccaacaacc tgacccgttc
     1021 gtcgaattac gtgaaaaata catcgcagat atgctttata aattagatgg cagagcctct
     1081 gaaagaattc ggaattcaat attaagtaga gtaaattaaa tttaattaat aatatgaatc
     1141 aggagtattt atgaaagtaa taatattagc ggcgggaaat gccattcggt tagcttctct
     1201 cgctaatgga agtcctaagt gtcttttgcc gcttggcaat acgacaattt taggtaaaca
     1261 attacacaat ctggaaaaag tcgggttaac agatgtgatt ctatcggttg gttataaagt
     1321 cgaaaaagtc gaagagtatc tgaaaaactt tcggactaat ttgaaaatcc atctcgaatt
     1381 caatccgttt tatgacattt ccaataatat cgtttcactc tggaacgtcc gtcacttggt
     1441 atggaatcag ccaattatta tcgttaacgg tgataatgta tttgattatc aaatactttc
     1501 gcttttactg gaatcacctt ttgaaaatgt tctgatgatc caagaaaaag atgaatatga
     1561 cggtgatgat atgaaggtca aaaccgaaaa tgatcggctt ctggctgtta ataaaactat
     1621 gaaaagttca gaagccaacg gtgaatctat cggaataatg aaattttcgg ccgaaggttg
     1681 tgcacaactg atgcaaaaaa tagtggaaat gactcgcaat aaggaaaatt tgcaggtttg
     1741 gtatttacga tcaatccagg aattgataaa tgatggctta ccggttcacg tttgccgaat
     1801 aaacggattg aaatgggaag aagtggactt cccggaagat tatttgcgcg taaaaaatat
     1861 gaattgggaa ggtaattgat cgggaaaaaa tatgtcaggt aaaataaaat tggatgacaa
     1921 atatctggaa aaatcgctaa gactgcttga gcgcgccaca acacttctgg agaaatataa
     1981 tattccatac tggctggaag gtgggactct tttaggaatt atcagagaaa atagactgct
     2041 tccctgggat aacgacctgg atatttccat ccgttccgaa gactttgatc gtttacggcg
     2101 tattctgcct aagttctact accggggaat gatcgctaaa gtcagagagc acaaaatcga
     2161 tgatcccccg ttccaaaaag gagaggtccg gctaataaag gtttatgcca ccaaatatct
     2221 atttttcaag actccgctcg ttctggacat cttcgttaag aaaaaattag atgatcaata
     2281 ctattgggtg gttggagtaa agcggcgggc aaagaaagcg gttccggctc gtttttatga
     2341 tgaactgaca accgttcagt ttaataataa aacttattcg attccgaaat tgactgatga
     2401 atatctgact tatcgttatg gtgattggcg cactccggtc aaaacctgga attatataaa
//
LOCUS       DAOMJU010000139         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_50545_length_2453_cov_18.226439, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000139 DAOMJU010000000
VERSION     DAOMJU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50545_length_2453_cov_18.226439"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLO58_09200"
     CDS             complement(<1..1115)
                     /locus_tag="PLO58_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQO75002.1"
                     /translation="MKIRTFIIFLTIVLLAYFIVNYYIFIHGLKAFGANQTAKLIYTI
                     LFVLLVSAYFLGRIIERISNGVVSQALEWLGAYWFGAMVYFLLIVIIIDIFGVIYHFI
                     PLIPNDLRQYLQQLNLITGIVTVTVVAIILVIGRWNATHPQIKTVGIDIHKKAKAETM
                     TIAFASDIHLGSFVGQRQLRQLVELINANQPDVILFGGDLLDEDLAPVIRHNLGGCLA
                     ELKAPLGKFTVPGNHEFIGGIEKATQYLENHGIKVLRDEALQLANGVWIIGRDDYDSK
                     RYSSYVQRLTLKYLIDKINPEQPIIVLDHQPLNLSESIASRIDLQLSGHTHNGQLWPL
                     NYLTAKVYQVSHGYKKIDGTHFIVSSGFGTWGPPIRLA"
     gene            complement(1158..2006)
                     /locus_tag="PLO58_09205"
     CDS             complement(1158..2006)
                     /locus_tag="PLO58_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQO75003.1"
                     /translation="MNCLKTIIISLVLFFNVINVLGDEPAATVIPDWSLKQVFKVPES
                     ALYDPERDVIYVSNINGSPSAADGNGFISRVSPNGKILDLHWIEGLNAPKGMAIYRDT
                     MYVADLNAIARIDLKQGRLIGKIDLKGKFLNDITVDENGTIYCSDNVADIIYIVKNGQ
                     PAIWLSGGNYNPNGLLAEKNQLVMLSSSNGTLNYIDYKNKSVRKVATIGGSLDGVVAC
                     DNNYLVSSFPGEIYLVSPDGQSKKILDTKTNKISSADIGYIPQKRLLLVPTFSDNSLM
                     TYKIVR"
     gene            2170..>2453
                     /locus_tag="PLO58_09210"
     CDS             2170..>2453
                     /locus_tag="PLO58_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HQO75004.1"
                     /translation="MNKTELIRTDEYIIHSYEVDCERRLRLSVLNRYMQESAWQHAET
                     LGAGYASLLSKNFAWVLTRTVISIEELPIWGNSLKLSTWPSGLDHLFAYR"
BASE COUNT          749 a          516 c          463 g          725 t
ORIGIN      
        1 gcaaggcgaa tcggcggtcc ccacgttcca aaaccgcttg atactataaa atgggtgcca
       61 tcaatttttt tataaccatg actaacttga taaacttttg cggtaaggta atttaatggc
      121 cacaattgtc cattatgggt atgtccagat aattgcaaat ctattcgact cgctatactt
      181 tcggataaat ttaacggttg atgatcaaga acaatgatcg gctgttcagg attaattttg
      241 tcaatcaagt atttcaatgt aagtcgctga acataactac tatagcgttt gctatcgtaa
      301 tcgtcccgac cgattatcca gacgccatta gccaactgca acgcttcatc acgtaagacc
      361 tttatcccgt gattttcaag atattgggtg gctttttcaa tcccgccgat aaattcgtga
      421 ttgccgggaa cggtaaattt ccccagcggc gctttgagtt ctgcgagaca gccgccgaga
      481 ttgtggcgaa ttactggcgc caagtcttca tccagcagat ctcctccaaa taaaataaca
      541 tccggttggt tggcattaat taattctact aattgacgta attgacgctg gcctacaaat
      601 gaaccaagat gaatatcact agcaaatgca attgtcattg tttcagcttt ggctttttta
      661 tgtatatcaa ttcccacagt tttgatttgc gggtgagttg cattccagcg tccaattacc
      721 aaaataatcg caaccacggt aacagtgaca atgccggtaa ttaaattcaa ctgctgaaga
      781 tattgccgta agtcattcgg aatcagcgga ataaagtgat aaatgacacc gaagatatcg
      841 attattataa caattagtaa aaaataaacc atagcgccga accagtaagc tccgagccat
      901 tccaacgctt gcgagacaac tccgttggaa attctctcga taatccgccc gagaaaatag
      961 gctgaaacca gaagaacgaa taaaatcgta taaatcaact ttgccgtttg gtttgcccca
     1021 aaggctttaa gtccgtgtat aaatatgtag taattgacaa taaaataagc taatagaaca
     1081 atcgtgagga atatgataaa cgtccgaatt ttcacgtaaa atctaaacct aattctttat
     1141 tattaaacgg attaaatcta acgaacaatt ttatacgtca tcaggctatt atctgagaaa
     1201 gtgggtacta atagtaaacg tttctgggga atgtagccaa tatcagccga cgagatttta
     1261 ttagttttag tatccaggat ttttttactc tggccgtccg gacttaccaa ataaatctct
     1321 cccgggaaac tagatacaag gtaattatta tcacaggcta ccacaccatc cagagagccg
     1381 ccaatcgtag ctactttacg aacagatttg tttttataat caatgtaatt taatgtgcca
     1441 ttgcttgaag aaagcatcac aagttggttt ttctcagcca gtaaaccgtt tggattgtag
     1501 ttgccgccgg agagccagat tgctggttgt ccatttttca cgatataaat aatatcagcg
     1561 acattgtccg aacaatagat tgtaccgttc tcgtcaacgg taatgtcgtt tagaaatttg
     1621 cctttcaagt caattttacc gatgagtcgc ccctgcttta gatcaatccg tgcaattgcg
     1681 ttcaaatcag cgacatacat tgtatctcga taaatcgcca tacctttcgg ggcattcaaa
     1741 ccttcaatcc agtgcaaatc cagaatttta ccattcggcg atactcgcga aatgaaaccg
     1801 ttaccgtctg ccgccgacgg actgccgtta atattactga cataaatgac atctcgttca
     1861 ggatcgtaca gcgctgattc cggtacttta aaaacctgtt tcaaactcca gtctggaata
     1921 acggtagcag ccggttcgtc acccaagacg ttaattacat tgaaaaaaag caccaaagaa
     1981 atgataattg ttttcaaaca attcataatc tatcctccat cttcttcatt gatttaatct
     2041 taaaaatcct tgagcaacaa atcaacaatt ttttaatgag gtaattgcag aaaattaaca
     2101 ggtacaaatg gtttgtcgga atttattttc actgaaagtt tgttatatta tctatcgttt
     2161 gaaaaaatta tgaataagac cgaactaatc agaactgacg agtatattat tcattcctac
     2221 gaagttgatt gcgagcgacg cctgcgactt tcggtgctga atcgttatat gcaggaatca
     2281 gcctggcaac atgcggagac gctgggcgct ggctatgcca gtctactgtc taaaaatttc
     2341 gcctgggtgc ttactcgaac tgtcattagc atcgaagaat tgccaatttg gggcaattca
     2401 ttgaaacttt cgacctggcc ttccggattg gatcatctct ttgcctatcg tga
//
LOCUS       DAOMJU010000140         2388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_52650_length_2388_cov_21.615945, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000140 DAOMJU010000000
VERSION     DAOMJU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52650_length_2388_cov_21.615945"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLO58_09215"
     CDS             <1..279
                     /locus_tag="PLO58_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO75005.1"
                     /translation="PAEITCVITPGDSQLKWVKIFLRDDSSELYKEFNMQYRDGIWFL
                     PLIPEMVSGNYLDYFIIAEFEDFTALAFPPERPAEKPIRVQIVSTKKI"
     gene            343..1659
                     /locus_tag="PLO58_09220"
     CDS             343..1659
                     /locus_tag="PLO58_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HQO75006.1"
                     /translation="MRSRFYLPKIFLILLAGSILLTATPKEIWVLEVDGVINPVSASY
                     INDNLRKAQPDQVECLIIQMDTPGGLMTSMRDIIKNILNSEIPVVVYVSPRGAQCASA
                     GVFIAVSAHFVAMSPGTNIGAAHPVNLGGGGIGGQPDSASSQTMIEKATNDAVAYIRS
                     LARERGRNEEWLEKAVRESASITETEAVKLQVVDVVVDDLDELLEILDGKTFKLPSGE
                     KTIHTQDATIRFRPVGIHHRILDVISDPTVAYILLMLGFYGIYFELSNPGAIFPGVLG
                     AIFLILAFFAFQVLPINYAGLALIILGIILFILEVKIISYGLLTIGGIVAMTLGSLML
                     IDVNQAPEILEAVSLKVILPIVIFTAAFVIISLTLALKAHKAKPTTGIEGMIGETGQT
                     ITDVDKTGMAIVHGEYWKVSSDTPIPKGTEIVVLDVDRMVLKVKPK"
     gene            1688..>2388
                     /locus_tag="PLO58_09225"
     CDS             1688..>2388
                     /locus_tag="PLO58_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HQO75007.1"
                     /translation="MSIPLITIIILLIFLISSAIRVLREYERGVIFRLGRLIAAKGPG
                     LFFLIPVVDRMVKVSLRTVVMDVPPQDIITKDNVSVKVNAVVYFRVVDPSKAVVEVED
                     YLYATSQLSQTTLRSILGQAELDELLSSREKINAELQTILDTHTDPWGIKVSQVEIKH
                     VDLPQEMQRAMAKQAEAERERRAKVINAEGEFQAATKLAQAAEIMNKEPITIQLRFLQ
                     TLREVAAENNSTTIFP"
BASE COUNT          659 a          513 c          531 g          685 t
ORIGIN      
        1 ccggcagaaa ttacctgtgt gattacgccg ggcgacagtc aattaaaatg ggttaaaatt
       61 ttcctgcgcg atgattcttc tgagttatat aaagaattta atatgcaata tcgggatggc
      121 atctggtttc tgcctctaat cccggaaatg gtcagtggta attatttaga ttactttatc
      181 atcgcggagt ttgaagattt tactgcgctg gctttccctc ccgaacgacc ggctgaaaag
      241 cccatcaggg tccaaatagt cagcactaag aaaatttaat ctcaccgact tttaaaaaaa
      301 gatttgagat tcacgcctat acggattata ttcaatataa ttatgaggag tcgcttttat
      361 ttaccgaaaa ttttcttgat cttgctggct ggcagtattt tgctgaccgc gactcctaaa
      421 gaaatttggg tgctggaagt tgacggagtt atcaatccgg tttcagcttc ctatatcaac
      481 gacaatttgc gcaaagctca acccgatcag gtcgagtgtc tgataattca gatggacact
      541 cccggcgggc taatgacttc tatgcgggat ataatcaaga atattcttaa ctcagaaatt
      601 ccagttgttg tttacgttag tccgcgcggg gcgcagtgtg cttccgcggg agtatttatt
      661 gccgtttcag cccattttgt ggcgatgtcg cccggaacga atattggcgc ggctcatccg
      721 gtcaatctgg gtggcggagg aatcggcggc cagccagatt ctgctagttc tcagactatg
      781 attgaaaaag ccactaatga tgcggtcgct tatatccgaa gtcttgcccg cgaacgtggt
      841 cgtaatgaag agtggctgga aaaagccgtg cgcgagagcg cttccattac cgagactgaa
      901 gccgttaaac ttcaggtcgt ggatgtcgta gtggatgact tagatgaact attagaaatt
      961 ctcgatggga aaactttcaa acttccttcc ggtgaaaaaa ccatccacac ccaagatgcg
     1021 actatccgat tccgtccggt tggcatccac catcggattc tggatgtaat cagtgatcct
     1081 actgtggctt atattctact aatgctgggc ttttacggaa tttattttga attgtctaat
     1141 cctggtgcaa tttttcctgg agtgctgggt gccatttttc ttattttagc atttttcgct
     1201 ttccaggttc taccgatcaa ctatgctggt ctggcgttga ttattctcgg tattatactc
     1261 tttatacttg aagtaaaaat aattagctac ggattgttaa ccattggagg gattgttgca
     1321 atgacactcg gttcattaat gttgatcgat gtaaatcaag ctcctgaaat tttagaagcc
     1381 gtctcgttaa aagtgatact gccgattgta atttttacgg ccgcttttgt tattatatct
     1441 cttacgctag cactcaaggc tcataaagcc aaaccaacta ccggaattga aggaatgatt
     1501 ggcgaaaccg gccagacaat taccgacgta gataaaaccg gcatggcaat cgttcatggc
     1561 gaatactgga aggtcagttc cgatacaccc attcccaaag gaacggaaat tgttgtgctt
     1621 gatgttgata gaatggtttt gaaagtcaaa cccaaataaa taacaagtaa caagtaagga
     1681 gattaagatg agcattccat taataactat tattatcctg ctgatttttc taatttccag
     1741 cgcaattcgg gttctgcgtg aatacgaacg tggtgtcata ttccgcctag gccgcttgat
     1801 tgccgcaaag ggaccaggac tctttttcct tatcccggta gttgatcgga tggtcaaggt
     1861 aagtctgcga acagtggtta tggacgtacc accgcaggat attatcacca aagacaatgt
     1921 atcagtcaaa gtcaatgcgg tggtctattt ccgggttgtc gatccctcca aagccgtcgt
     1981 cgaagttgag gattatttat atgccacttc tcaattatct cagacaactc ttcgtagtat
     2041 tttaggacag gctgaactcg atgaactgct ttccagtcgt gaaaaaatca acgcggaact
     2101 gcagacaatt ttagatactc atactgatcc ctggggcatt aaggtttcac aagtcgaaat
     2161 caaacacgtt gacctacccc aagaaatgca aagagcaatg gctaaacagg ctgaagccga
     2221 acgggaacgc cgcgccaaag tcatcaatgc cgaaggcgaa tttcaggctg ccactaaact
     2281 tgcccaagcc gcagaaatta tgaacaaaga gcctattaca atccaattac gctttctgca
     2341 aactttacgc gaagttgccg ctgaaaataa ttcaacgacg attttccc
//
LOCUS       DAOMJU010000141         2374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_53108_length_2374_cov_17.963346, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000141 DAOMJU010000000
VERSION     DAOMJU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53108_length_2374_cov_17.963346"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLO58_09230"
     CDS             <1..632
                     /locus_tag="PLO58_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQO75008.1"
                     /translation="VVLQLKKNGFRTAGFANASGFYSFLNEKSPDLVILDLMLPDIDG
                     VEICRQLRSNPQTAKIPIIMLTARSEEFDKVLGLEIGADDYVTKPFSTRELVARVKAV
                     LRRSQPTLSENILKITDSLTIDLEKYIVQADGKPIALTATEFNLLKILAQKPGHVFSR
                     EHLLDQLWGEDKIVVNRTIDVHIKNLREKLGEYGSLIRNLRGVGYKLEI"
     gene            629..2347
                     /locus_tag="PLO58_09235"
     CDS             629..2347
                     /locus_tag="PLO58_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQO75009.1"
                     /translation="MKKSFFYRIFSGYLILIVALTVLIVALSYNSLRRHFVEAQIADL
                     QDLNASLSVLLLSAKNQSREHLDHLTKSLGNEIRARITIIDTTGNVIADSESDPRLMK
                     NHRQRPEIKQALRGNVAHSQRFSTTVEENMLYVASPLVQDGQTVAVVRTSIYLSHLND
                     IFKQILNRIIKIAILIILIALSAAFLLARYYSKPVNQLVTAARQIGQGKLETRVNIQT
                     SDEFAELADSFNAMAVEINRLLADVARQRDELQTIVAAVPSGLLVLTADGRVAFYNRE
                     FQKITEREELQDRFYWEILRQPQFTEFIKDIQASRANRTQEIVINNKTYLCSAEAETS
                     SAEIVLIFSDISELKAVQQMKRDFVVNVSHELRTPLTAIKGFVETLQEEASGNEHRYL
                     TIIARHTDRLINIVNDLLTLAEIEEKNQLEYETFSLSNLINDTVKIFEERLQAKNLKM
                     IVEIADGLTTVKVDPFKFQQVLINLLDNAIKYTEQGQITIRVQKKADQVQIVIQDTGC
                     GIPPEHLPRLFERFYVVDKARSRQMGGTGLGLAIARHIVLLHNGTIEVESQPGVGTTF
                     FINIPA"
BASE COUNT          680 a          489 c          554 g          651 t
ORIGIN      
        1 tggtagtttt gcaactcaag aaaaatggtt tccgaactgc gggatttgcc aatgccagtg
       61 gattttatag ctttctaaat gaaaaatcgc ctgacttggt cattctcgac ctaatgttgc
      121 cggacattga tggggtggaa atctgtaggc aactgagaag caatcctcaa acggctaaaa
      181 ttccgattat tatgttgact gctcgcagtg aagaattcga caaggttctc ggattggaaa
      241 tcggtgccga cgattatgtc actaagccat tttcgacgcg agaattagtt gcgcgagtga
      301 aagccgtttt acggcgctcg caaccgacct taagtgagaa tattttgaaa atcaccgaca
      361 gtttaacaat tgatctggag aaatatatcg ttcaggctga tggcaaaccg attgcattaa
      421 ccgccactga gtttaatctc ctgaaaatat tggcgcaaaa accagggcac gttttttcac
      481 gggagcatct gcttgatcaa ctttggggtg aagataaaat tgtcgtcaac cgcactattg
      541 acgttcatat caaaaacttg cgggaaaagc tgggcgaata cggtagcctg atccgcaatt
      601 taagaggagt tggttacaag ctcgaaatat gaaaaaatct tttttttacc gaatattttc
      661 cggctacctt attctgattg tagcgctgac ggtcttaatt gtagcgctgt catataattc
      721 actccgcaga catttcgttg aagcccagat cgctgacctg caggatttga atgccagtct
      781 atcggtttta ttgttatcgg ctaaaaatca aagccgtgag catttggatc atctgaccaa
      841 gtcgcttggg aatgaaatcc gggctcgcat cacaattatt gataccaccg gtaatgtcat
      901 tgccgattct gagtctgatc cgcgcctgat gaaaaaccat cgccagcgac cggaaatcaa
      961 gcaggctttg aggggtaatg tggcgcattc tcaacgtttt agcacgacgg ttgaagaaaa
     1021 tatgctgtat gtcgcttcac cgctggttca agatggacag actgtagcag tcgtgcgtac
     1081 gagcatttat ttgagccatt tgaacgatat tttcaagcaa attctcaatc gaattatcaa
     1141 aattgctata cttataattt taatcgcgct gagcgcggct tttctgctgg cgcgttatta
     1201 ttccaaaccg gtcaatcaat tggtcacagc cgctcgtcaa attgggcagg gcaaactcga
     1261 aacccgtgtt aatattcaga ccagtgatga atttgccgaa ctggctgata gttttaatgc
     1321 gatggcagtt gagattaatc gcttgctggc ggatgttgcc cgacaacgtg atgaattgca
     1381 gacaatcgtt gcggcggtgc cttccggttt actggtactg actgccgatg ggcgggtggc
     1441 attttataat cgtgaatttc aaaaaattac tgaacgggag gaactacagg acaggttcta
     1501 ctgggaaatt ttacgtcagc cacagtttac cgagtttatc aaagatatac aggcaagtcg
     1561 agctaatcgc acccaggaaa tagttataaa taataaaacc tatttgtgca gtgctgaggc
     1621 ggagacgtcc agcgctgaaa ttgttctgat attttctgat atttcagaac tgaaagccgt
     1681 tcaacaaatg aagcgtgact tcgtggtcaa tgtctcgcac gagttgcgta cgccgctgac
     1741 tgccatcaaa ggtttcgtcg aaacacttca agaagaagcc tccggcaatg agcatcgcta
     1801 cctgacaatc attgcgcggc ataccgatcg cttgatcaat attgtcaatg acttgctgac
     1861 gctggcggaa atagaagaaa aaaatcagct ggaatatgag acttttagtt tatcgaattt
     1921 gattaatgat accgttaaaa tttttgaaga acgcctgcaa gcgaaaaatc taaaaatgat
     1981 agtcgaaatc gcagatggct tgacaacagt taaagttgat ccattcaaat ttcagcaggt
     2041 tttaattaat cttctggata atgccataaa atacaccgaa cagggacaaa tcactattcg
     2101 ggtgcaaaag aaggcagacc aggtccaaat tgttattcag gataccggtt gcggtattcc
     2161 accggaacat ctaccgcggc ttttcgagcg gttttacgtg gtggataaag cccgctcgcg
     2221 tcagatgggc ggaaccggtt tgggattagc aattgccaga catattgttt tacttcataa
     2281 tggtacgatc gaagtcgaga gccaaccggg tgtcggtacg acctttttca ttaatatacc
     2341 agcgtagata atctttcgca aaaccacaag ggca
//
LOCUS       DAOMJU010000142         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_55956_length_2295_cov_18.183482, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000142 DAOMJU010000000
VERSION     DAOMJU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55956_length_2295_cov_18.183482"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PLO58_09240"
     CDS             <1..1026
                     /locus_tag="PLO58_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQO75010.1"
                     /translation="LARESPMESHLIINVDRAPDYFLLANLQGDDARVFVAEQDGVIV
                     GAIGCCLREVLLAGQPTRIGYIGGIKVAAAARKGLTSFRLMNAVANYLRAVPVEMAIV
                     ITMTDNEAMAPILAGRVGMPPFHPINRFVINYIFPLFQPQVTGGYQIRSFRESDLDHL
                     TRLFTDFFRNYSLTFEWTPKRLSALLNQPNFSSENILIATKNGRPVAALTWWDQSSFK
                     RTIVEKYDGWLKTLAGLSKPLKIFPQAGEPLIELQIRHIVYEKEHTSAARDLIRYLIN
                     EKKRKYRLFRIGFQQNSPLAALLSGLPHLKIHLNCYIASDKKDDETLLLISALRQGQI
                     WEDLSLH"
     gene            1023..1964
                     /locus_tag="PLO58_09245"
     CDS             1023..1964
                     /locus_tag="PLO58_09245"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQO75011.1"
                     /translation="MKKEKSISIICNLQACGGKARRRWLDFLKIATQAGFSIDFHITE
                     YHGHATVLARERLAKGADRIIVFGGDGTLNEALNGIIVKDRPVNPDLRLVYLTAGSSC
                     DVAKMFPNPQTLLQRIADGKDYSVDVGKLECLNARAEKVVRYFLANSSIGVISRSIEK
                     FNQKRPTMNLLKRINIDLAAVSAGIKTIIQFENIDSRISYENQPFTTQKIKNITVFKS
                     PYFGGGMNYGVPTSYIDHFLHVATIAAMDAVHTFRMIPLLYSGTILKTPRAEYCRVQN
                     LSFDAPGQHIPIEADGEIIGFPPCHYTILPELIHLVV"
     gene            complement(2085..2157)
                     /locus_tag="PLO58_09250"
     tRNA            complement(2085..2157)
                     /locus_tag="PLO58_09250"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2122..2124),aa:Thr,seq:cgt)
BASE COUNT          637 a          522 c          509 g          627 t
ORIGIN      
        1 cttgcgcgcg agtcaccgat ggaaagtcac ctaatcatca atgttgaccg cgcgccggac
       61 tattttctac tggcaaattt acaaggcgac gacgcccgag tatttgtagc tgaacaggat
      121 ggggttatcg tcggtgctat tggttgctgt ctgcgcgagg ttttgcttgc cggacaaccg
      181 actcgcattg gttacatcgg cggaataaaa gttgccgccg ccgctcgtaa aggcttgacc
      241 agcttccgct tgatgaatgc cgtagccaat tatttacggg cggttcccgt cgaaatggct
      301 atagtaatta cgatgactga caatgaggcg atggcaccca ttttagctgg tcgggtcggt
      361 atgccgcctt ttcacccaat taatcggttt gtaatcaatt acatttttcc actttttcaa
      421 ccgcaagtta ctggtggcta ccaaattcga tcgttccgtg aaagcgacct cgatcatctg
      481 acacgattgt tcactgattt ttttcgaaat tactctttga cattcgaatg gacaccgaaa
      541 cgcctttccg ctttattgaa ccagccgaat tttagttcgg aaaacatact gattgccaca
      601 aaaaatggtc gcccggttgc ggcactgact tggtgggatc aatccagttt caagagaaca
      661 attgtcgaaa aatatgatgg ctggctaaaa acgctagctg gcttatcgaa acctttaaaa
      721 atatttccgc aggcgggtga accgttaatt gaactacaga tccggcatat cgtttacgag
      781 aaagaacata cctcagccgc acgcgattta atccgatatt taattaatga aaaaaagcgt
      841 aagtaccgcc tctttcgcat cggttttcaa caaaattcac ctctcgcggc tttgttatcc
      901 ggattaccgc atctgaaaat tcatctgaat tgctacattg cctcggataa aaaagacgac
      961 gaaaccctct tgctaatttc cgccctgcgt caaggtcaaa tctgggagga tctgtcgtta
     1021 cattgaaaaa ggaaaaaagc atttccatca tttgtaacct gcaagcctgc ggtgggaaag
     1081 cccgtcggcg ctggctggat ttcttgaaaa tcgcaaccca ggctgggttc agtatagatt
     1141 ttcatatcac ggaatatcac gggcatgcga cggttttagc ccgcgaacgt cttgccaaag
     1201 gagccgaccg gatcatcgtt ttcggtggcg acggtacttt gaacgaagct ctcaacggca
     1261 taattgtaaa agaccgtccg gtgaatcccg acttgcgttt ggtttacctt acggctggca
     1321 gtagttgcga tgtggctaaa atgtttccca acccccaaac acttctacaa cggatcgccg
     1381 atggcaagga ttattcggtt gatgtgggca aacttgaatg tctgaacgca cgggctgaaa
     1441 aggttgtgcg ctattttttg gctaattcta gcattggagt aataagtcgt tccattgaaa
     1501 aattcaacca gaaacgccca acgatgaatt tgctgaagag aattaatatt gatctggcgg
     1561 cggtcagtgc cgggattaag accattatcc aattcgaaaa tatagacagc cgcatctcct
     1621 atgaaaatca gccctttacc acccaaaaaa tcaaaaacat cactgttttc aaatcgccct
     1681 atttcggcgg cggtatgaac tacggtgtgc caactagtta tattgatcat ttcttgcacg
     1741 tcgcgacgat tgccgcgatg gatgcggttc atacttttcg aatgattcct ttactttact
     1801 ctggaaccat cttgaaaacg ccgcgagctg agtattgtcg ggtacaaaac ctgagttttg
     1861 atgctcctgg tcaacatatt cccattgaag ccgatgggga aattatcgga tttccgccct
     1921 gccattatac cattcttcca gaattaattc acctagtcgt ctaaacttta taacaaattg
     1981 aactgatatt taaagtctga ggtacaactg ttggcggtgg tgtggggaag actatccaaa
     2041 aatagccaac tgaaaaaatt ttctaactgt aagaaaatat tttgagccac ccaacgggat
     2101 cgaaccgttg acctgacgat tacgaatcgt ctgctctacc agctgagcta gggtggcaaa
     2161 aagcgaccca aatttatcat atttattcgg ttcatacaaa acaggcaatg tgcacgggag
     2221 cggttagtta aagattaaga tttctcttag atatagttta ggtaccaacg ctcggaaaaa
     2281 attaaatcaa acaca
//
LOCUS       DAOMJU010000143         2218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_58902_length_2218_cov_20.035599, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000143 DAOMJU010000000
VERSION     DAOMJU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58902_length_2218_cov_20.035599"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..857
                     /locus_tag="PLO58_09255"
     CDS             87..857
                     /locus_tag="PLO58_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQO75012.1"
                     /translation="MTKKPFYISIICCLLIKTGLNGAELTLKLPPPNPIAQPLQYLQY
                     YPPHYWNGLQKTLDWKSNAPLFVAGAILIPMSFLGDQPLQENVAKHNFLPENISRIGY
                     IYGHRWGNYAAAVAVGINGVIQHQPWNKTFSQIELLATSTITTALVTEAIKLATHRER
                     PNEENYKSFPSGHSSNSFALAAGLDGIYGHKIGIPAYLMAIFVASTRINDNKHYLSDV
                     VAGAFIGTWIGKSFSRQYQLEWQISATGSPAISITIPF"
     gene            869..1762
                     /locus_tag="PLO58_09260"
     CDS             869..1762
                     /locus_tag="PLO58_09260"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQO75013.1"
                     /translation="MSFLSAVQQARFLILDGALGTELARRGIAIELPLWSANAILHNP
                     QAIVEIHSDYIRAGADIITTATFRTDSLTFTRAGLTEKQAAEATFRAVELAREAIADV
                     HSTRQIWIAGSIAPLADCYEPESAPDFNTALRIHRQRAQWLAEAGVDFALLETMNNLS
                     EARAASIAALETGLPVFTSFILAENGVLLSGEDIWLVMSEIKKLGVAGLGINCTHHSL
                     ISNFLENYRSKFDLPLILYANAGIYSRNTGWRQDPDFTPQNYARIAANWLKSGAKIIG
                     GCCGTTPDFIKALADLRKSPV"
BASE COUNT          615 a          502 c          474 g          627 t
ORIGIN      
        1 ctctattatt ttttgatatg ttttgagttg tattaaaact taaattactt ccgaattgtc
       61 tgtactttag aaaaaatatg aaaaaaatga ccaaaaaacc attttatata agtatcatct
      121 gttgcctttt aattaaaacc ggattgaatg gcgccgaact tacgctgaaa ttgccgccgc
      181 ctaatccgat tgcgcaaccc ctgcaatact tacaatatta tccacctcat tactggaatg
      241 gtttgcagaa aaccctcgat tggaaatcca atgcgccgct ttttgtagcg ggtgccatct
      301 taataccgat gtccttttta ggcgatcagc cactgcagga aaatgtcgcc aaacataatt
      361 ttttacctga gaatatttca cggatcggct atatttatgg acatcgttgg ggcaattacg
      421 ccgccgccgt ggctgtggga atcaacggcg tcatccagca tcagccctgg aataaaacct
      481 tttcgcaaat cgaattgtta gccaccagca ccataaccac tgctctagtt acagaagcaa
      541 ttaaattagc aacgcatcgg gaacgtccta atgaagaaaa ttataaatca tttccctcag
      601 gacattcctc caattccttc gcattggcgg ctggtctgga cggcatttac ggtcacaaga
      661 ttggcatccc ggcttatcta atggcaattt tcgtagcgtc cacccgcatt aacgataata
      721 aacattatct ttccgatgta gttgccggtg cgtttatcgg cacctggatc ggaaaaagtt
      781 ttagtcggca gtatcagctc gagtggcaaa tttcagctac aggatcaccg gcaatttcta
      841 ttactatacc attctgaagg ggaattttat gtcttttcta tctgctgttc aacaagcgag
      901 atttttaata ctggatggag ctctcggtac ggaattggca cgacgcggta tcgccatcga
      961 attacctctc tggtcagcca atgccattct gcataatcct caagcgattg tcgaaattca
     1021 ttcagattat atccgagccg gagcggatat tatcacgact gccactttcc gcaccgattc
     1081 actgaccttt actcgagccg gactgactga aaaacaagcc gcggaagcca catttcgggc
     1141 ggttgaactg gctcgcgaag ccatcgctga tgttcactcg acacgtcaaa tctggatcgc
     1201 cggttcgata gcgcctctgg ctgattgcta tgagccggaa tccgcacccg atttcaatac
     1261 ggcacttcga atccatcgcc agagagccca gtggttggcg gaagccggag ttgatttcgc
     1321 tctcctggaa acaatgaata atctcagcga agccagagcg gcgtctattg cggctcttga
     1381 aaccggactg ccggttttta ccagcttcat cctcgcggaa aatggtgttt tattgagcgg
     1441 agaagatatc tggttagtga tgagtgaaat caaaaaactc ggagtcgccg gactaggaat
     1501 taattgtact catcacagtc tgatctcgaa tttccttgaa aattataggt ctaaattcga
     1561 cttgccgtta attttatacg ccaatgccgg aatctattca cgtaatactg gctggcgcca
     1621 agacccggat tttacgccgc agaattacgc ccggatagcg gcaaattggc taaaatcagg
     1681 cgctaaaatc attggcggct gttgtggaac aactcctgat tttataaagg ctctggctga
     1741 tttaagaaaa agtccagtgt gatggggaat cacgctggac tggagggtag tgaggtttgg
     1801 agttttcaaa aaattctaac ctaatttaac ccaaaagccg agctggattt atgccacgcg
     1861 tcacaatttt cccgggcatt aatactattt tcaagacctt cgccaatttt ttagaatgtt
     1921 taacttctac gaagtaagga ttgctgataa ttgtattttt ctataaaact tccactacct
     1981 cagccaattt ctgccacacc gcaggcgggg ttaaattggt ttcgacgcaa tatttagagc
     2041 gagtggttaa tagcttagta ctcaccgcgt agcggtcaaa gtattgtaga atataaaaat
     2101 cagagaagtt attcctcgga gaggatcaag aagaaactat ttgccgaacg caatctttat
     2161 ttggccaaga ataactattg cgttatgcgc ttggctaata tttcaacaag atttcatc
//
LOCUS       DAOMJU010000144         2215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_59029_length_2215_cov_14.203241, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000144 DAOMJU010000000
VERSION     DAOMJU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59029_length_2215_cov_14.203241"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..846)
                     /locus_tag="PLO58_09265"
     CDS             complement(67..846)
                     /locus_tag="PLO58_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO75014.1"
                     /translation="MIKSIHPKESSTLLGFKKLLSIKRMKYYSPFLIIFIVLASVLSL
                     AAEIEKYYGKIDPGDGHIYTCSGEIIRNDGKVTKTSYFYDENGKILLNEKVVFDEKGY
                     KLINLVSEEPRYGRIQEIQRKGSQYIARYRKNSGEEFREYIINDQPLLLHSSNLPVYL
                     SQNLNLIGESGHVCRLIIVPMKMEIEMIFKNKGIKNIDGHQCFEIQMDAANIFLKPLV
                     KTHYFYYGTEAPHYLYHYIGTISLTNPNGDDIWGTISLTYE"
     gene            1012..>2215
                     /locus_tag="PLO58_09270"
     CDS             1012..>2215
                     /locus_tag="PLO58_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HQO75015.1"
                     /translation="MTPWDFTLNFKDMILDFTALGTFLVLGTIARRYIKFFQKYLVPN
                     NIIGGFLGLLLSQQVLGLIYLDENRLGQYVYHLLALTFIVIGLGKSKTKFGKGPVSKC
                     FTELTCYIVQGAIGLLVVLALIYTVSPDLPAAMGLLVPLGFGMGPGIAYTMGHSWEKF
                     GFEGGGMIGLTFAAIGFLIAYFAGVAIVNRGIKNRETTLIKGSESINNDIRTGVVKEN
                     KPEVAGWLTLSPEAIEPMAFQLGLISIVYAMTYGVIYFVTKLMTTYGLKDFVDTIWSF
                     HFVIALLIALLVRKVLDLTDKSYVIDSGLMNRISGVAVDFLVTGSIVAINIVIVGKYW
                     LPITLISILAGIATYFVIKYTSRRAFDDFHFERMVGIFGEMTGTINSGLVLIRITDPE
                     FQTPSAEDL"
BASE COUNT          620 a          398 c          470 g          727 t
ORIGIN      
        1 agcgcgtgct gaattcttga aattgattag ccggtaaaaa tcgtaacgaa ttactcagcg
       61 cacgttttat tcataagtaa gtgaaatcgt tccccaaatg tcatcaccgt taggattagt
      121 gagtgaaatt gtgccgatat aatggtataa ataatggggc gcttccgtac cgtaataaaa
      181 ataatgagtt ttaaccaatg gttttaagaa tatattagcg gcatccatct gaatttcaaa
      241 gcattgatgc ccgtcaatat tttttatccc tttgttttta aatatcattt cgatttccat
      301 cttcatgggg actataatca aacggcagac gtggccactt tccccaatta aattgagatt
      361 ttgtgacaga taaaccggca ggttagaact atgcaagagc aacggctgat cattgatgat
      421 atactcgcgg aattcctcgc cgctgttttt gcgatagcgg gcaatgtact gactcccttt
      481 tcgctgaatt tcctgaatgc gaccatagcg cggttcctcg gatacgaggt taatgagttt
      541 gtatcctttc tcatcaaaga ccaccttttc gttgagaaga attttgccat tctcatcata
      601 aaaataagaa gttttagtta ctttgccatc attacggatg atttctccac tacaggtgta
      661 gatatgaccg tcgcccgggt caattttccc ataatatttt tcaatttcag cagctaaaga
      721 taaaacggat gcaagaacga tgaaaattat tagaaacggc gaatagtact tcattctttt
      781 tatagataat aactttttaa agcccaagag ggttgaactt tctttaggat gtattgactt
      841 aatcatcatt ttataatttt ctactaaaaa gtaatttttc aaacgaaata gaaattatca
      901 aaatcagtcc ctatagagca aaaatatttt aaattgattt tataaattat tttagattac
      961 tatattaaaa gtataagaga cggttaatta aaatgatgaa aagaggaaat gatgactcct
     1021 tgggatttta ctctgaattt caaagatatg attttagatt ttactgctct ggggactttc
     1081 ttagttctag gaacgatcgc gcgacgctat attaagtttt tccagaaata tctggttccg
     1141 aataatatca ttggcggttt tttaggactt ctgcttagtc agcaagtatt gggattgata
     1201 tacctcgacg aaaaccggct ggggcaatat gtttaccatc tcctggcgtt aactttcatt
     1261 gtaattggtt taggaaaatc caagactaaa ttcggaaaag gaccggtcag taagtgcttt
     1321 acggaattga cctgttatat tgtccaggga gcgatcggtc tattggtggt tctggcatta
     1381 atctataccg tttctcccga cttaccagcc gcaatgggtt tattggtacc gctgggattc
     1441 ggtatgggtc cgggaatagc ttatactatg ggacacagct gggaaaaatt cggatttgaa
     1501 ggcggcggga tgatcggttt gacttttgcc gctattggtt ttttgattgc ttattttgcc
     1561 ggggtggcga ttgttaaccg gggtatcaaa aaccgcgaaa cgactctgat caagggaagc
     1621 gaatctatca ataatgatat tcgaaccggt gtcgtcaaag aaaataaacc ggaagtcgcc
     1681 ggttggttga cattatcccc agaagccatt gaaccaatgg ctttccagct tggactgatc
     1741 agtattgtgt atgcaatgac ctatggggta atttatttcg tcactaaatt gatgaccacg
     1801 tatggtttaa aggatttcgt tgatacaatc tggtcttttc actttgtgat tgctctgctc
     1861 atcgccttgc tagttcgtaa agttcttgat ctgactgaca aaagctatgt tattgacagc
     1921 ggtctgatga atcggatttc cggtgtggcg gttgattttc tggtgactgg ctcgattgta
     1981 gccatcaata tcgtaattgt cggtaaatat tggttaccga ttactcttat ttcaatttta
     2041 gctggaattg caacttattt tgttattaaa tacacttccc gacgagcttt tgatgacttt
     2101 cattttgaac ggatggtcgg gattttcggt gaaatgaccg gaacgatcaa ttctggtttg
     2161 gtgctgattc gtatcaccga tccggaattc caaacgccgt ccgccgagga cttgg
//
LOCUS       DAOMJU010000145         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_60690_length_2175_cov_18.487736, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000145 DAOMJU010000000
VERSION     DAOMJU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60690_length_2175_cov_18.487736"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..190)
                     /locus_tag="PLO58_09275"
     tRNA            complement(117..190)
                     /locus_tag="PLO58_09275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Arg,seq:acg)
     gene            complement(262..657)
                     /locus_tag="PLO58_09280"
     CDS             complement(262..657)
                     /locus_tag="PLO58_09280"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQO75016.1"
                     /translation="MEMIIDFPKGLRVDAHLGKFTIRTDQPLSSGGEETAPTPFALFL
                     ASLGTCAGAYVLSFCRQRNLPTEKIRLVQRTERDTATGMISRIQLEIQVPEDFPEKYY
                     DALVRSANQCIVKKHLENPPHFEVFTKVV"
     gene            826..2106
                     /locus_tag="PLO58_09285"
     CDS             826..2106
                     /locus_tag="PLO58_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HQO75017.1"
                     /translation="MKLLRKSFLIFITNLFVFITLNAQSLNKAVYKPRYHDPVLEQMK
                     EQREKDIAREDSLTAAIRTQQEARRKIDKKDELSLQSDLSGVKKPASLNVFKTIDHFP
                     PIAQYQSGMCWCFCGTSFLESEVMRLTGQKIKISELHTIYYEYLEKAARFIRERGDSF
                     IGEGGEVNGVLRIMKKYGAVPAEVYTGLPHGDKHDHTRLFTEIRNYLEYCKTNNYWDE
                     EIALNSIKLILNKYLGEPPQSFIYEGQEYTPVTFLNNVLKLQLDDYCDVMSTTAVPFY
                     IRAEFKVEDNWWHDSSYINIPLAEWYKFLKKAITGGYSLAIGGDVSEPGYLGFEDVAF
                     IPDFDIPFKYINQDSREFRIYNKTTTDDHGLHLVGYTRIDDRYWFLVKDSARSSRWGK
                     YEGYYFYREDYIKLKMLTYTVHKDVLKEILNQVK"
BASE COUNT          653 a          447 c          422 g          653 t
ORIGIN      
        1 cgaccaatta ttaattaata tcaactgaca ggtaggaacg cacctattgc agtttttttt
       61 actctaatta atctaaacaa aacagccggt tgtcaccggc tgtttttatt ttaatgtacg
      121 cccggaagga ttcgaaccct caacattcag gtccgtagcc tgacggtcta tccagttggc
      181 ctacgggcgc attactcaaa aagcgattta atttagaaca gatataattt tttgccaacc
      241 atttttttcc attttattca tttatacaac cttggtaaaa acttcaaaat gaggtggatt
      301 ttccagatgt tttttaacga tacattggtt agctgaacgc accagcgcat cataatattt
      361 ttcgggaaaa tcctcgggaa cctgaatctc taattggatt cggctaatca tacctgtagc
      421 cgtatcacgc tccgtacgtt gcaccagccg aatcttttcc gtcggcaaat tgcgttgtct
      481 gcaaaaacta aggacatacg ccccggcaca cgttcccagc gaagccaaaa acaatgcaaa
      541 cggagtcgga gccgtttctt cgccacccga gctgagcggc tgatcagtcc gaatggtaaa
      601 cttacctaaa tgagcatcta ctcgtaaccc tttcgggaaa tctattatca tttccattac
      661 tgactccttt ttgttaatta atttattgtt tttagataat ttttcctctt cagtggttac
      721 agttcgattc aattttcagt tgaaaacgtc ttatgaattt cttaacattt attgaaatga
      781 ttaaaacaat tttgaaatta ataaccacta aaacggagtt taaaaatgaa actattacgt
      841 aaaagttttc tgatttttat tactaacctt tttgttttca ttacattaaa cgcgcaaagc
      901 cttaataaag cggtatataa acccagatac cacgacccgg tactggaaca gatgaaagag
      961 caacgtgaga aagatattgc tcgtgaggat tcgctgactg cagcaattcg tacgcaacaa
     1021 gaagcgcgcc gaaaaattga caaaaaggat gaactttctc tccaatccga cctctccgga
     1081 gtaaaaaaac cagcttcgct caatgtcttt aaaaccattg accacttccc tcctatcgcc
     1141 caatatcaat ccggtatgtg ttggtgcttt tgtggaacat cttttttaga atcggaggtt
     1201 atgcgtctga ccggacaaaa aatcaaaata tcggaattac ataccattta ctatgaatat
     1261 ctggagaaag cggcgcggtt cattcgcgaa agaggcgatt cattcattgg ggaaggtggc
     1321 gaagtcaacg gcgttttacg cattatgaaa aaatatggag cggttccggc ggaggtctat
     1381 actggtcttc ctcacggtga caaacacgat cacactcgcc ttttcacaga aatacgcaat
     1441 tatttggaat attgtaaaac caacaattac tgggacgaag aaatcgctct taatagtatt
     1501 aaactgattc taaataaata tctcggcgaa ccaccccaga gttttatcta cgaaggtcag
     1561 gaatatactc cggtaacttt tttgaataat gttctcaaat tgcaactcga cgattattgc
     1621 gacgtaatgt ccacgaccgc tgttccgttt tacatccgag cggaatttaa agtcgaggac
     1681 aattggtggc atgattcgtc ttatatcaat attccactgg cagaatggta taaatttttg
     1741 aaaaaagcga ttaccggggg atatagtctg gcgattggcg gcgatgtcag tgaaccgggt
     1801 tatctgggct ttgaggatgt agctttcatt ccggatttcg atattccgtt taaatatatt
     1861 aatcaggact cccgcgaatt tcggatttat aataaaacta cgactgacga tcacggtttg
     1921 catcttgtcg gatatacccg cattgacgat cgttactggt ttttagtaaa ggactcggct
     1981 cgcagttcac gttggggaaa atatgaaggc tattattttt atcgcgaaga ttacatcaaa
     2041 ttgaaaatgc ttacctatac tgtccataaa gatgttttga aggaaatttt aaatcaggta
     2101 aaatagatag cctttatgaa aagccgtggg cttaaccccg taggacagcc tttccaggct
     2161 gtcacttaga tatta
//
LOCUS       DAOMJU010000146         1983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_69636_length_1983_cov_13.991183, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000146 DAOMJU010000000
VERSION     DAOMJU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69636_length_1983_cov_13.991183"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="PLO58_09290"
     CDS             <1..143
                     /locus_tag="PLO58_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HQO75018.1"
                     /translation="LIGIVFIFLTSPVAAHVIARAAHRSGIKLTKESVVDQYQEDREL
                     KE"
     gene            154..756
                     /locus_tag="PLO58_09295"
     CDS             154..756
                     /locus_tag="PLO58_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQO75019.1"
                     /translation="MYLPKLRELSEALRSLFSLPFTTKFPAKPHQPADGFRGFPEYYK
                     EYCVGCGTCAQVCPTGAISVTDDIAAHKRILRVDYTSCAQCGQCQEKCITGKGIQLSK
                     NYLLAISDLKDQAVYNTVEKDLALCEACGTKIACFDQLLFVKERLGAKSYANPNLLLV
                     TQQQFSEVPDSHVKDRLRREDYVKMVCPKCRHKIVVKDEF"
     gene            746..1213
                     /locus_tag="PLO58_09300"
     CDS             746..1213
                     /locus_tag="PLO58_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HQO75020.1"
                     /translation="MNFNDLKKTLAVYHSDRIIFVGLGNRDCGDDGASQILLNKLKHS
                     EEFKEAGFIFAGTNPENYLQKITDKNPQAVVFIDAAHFQDQSGTVKFLEAKQLKPSDF
                     STHAFSISLIAEYLTYEKSCAIYYLGIQPENSGVGQSLSESVQSGLAHFFSES"
     gene            1224..>1983
                     /locus_tag="PLO58_09305"
     CDS             1224..>1983
                     /locus_tag="PLO58_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO75021.1"
                     /translation="MATNEPYKLILPFHTLETMQTRISWLVNLRWIGIISLLGIIPIA
                     EQVLKFSLGFPQIYTLAVVFIVLNVTYFFICKYFKFVNFGQEIAFTEIQIIFDFVLIS
                     FVVHYTGGIDNPFYFIYLVHIIISGILYQGIWPYINATVAAFLLTLWSGLEYFKVVNV
                     YSLSGSPAPIKVYLVSLAAFYILIYTASYIIKDIVSGYRMLKRVIDQKSELLEQTMLE
                     RDKMFRFTAHELKSPLTTIRSMLAVIDEVYSDSLQ"
BASE COUNT          550 a          395 c          434 g          604 t
ORIGIN      
        1 tgttgatagg gattgtattt attttcctga cttcgccagt ggcggcgcac gtcattgcgc
       61 gggcggcgca tcgcagtggt attaaactta ctaaagaatc cgtcgtggat cagtatcaag
      121 aagaccgtga attaaaggaa tagggagact gcaatgtatt tgccgaaatt aagggaatta
      181 agcgaagcct tacggtcgct tttttcatta ccgtttacta ctaagtttcc ggctaaaccg
      241 catcaaccag cggatggttt ccgcggattc ccggaatatt ataaagaata ttgcgtcggt
      301 tgcggtacct gcgcccaggt ttgtccgacg ggcgctattt ccgttacgga cgatattgcc
      361 gctcataagc gtattctgcg ggttgattat acttcctgtg ctcagtgcgg ccagtgccag
      421 gaaaaatgta tcacaggtaa gggcattcaa ctttcgaaaa attacttgct tgctatctcg
      481 gatttaaaag atcaagccgt ctataatacc gtggagaaag atctggctct ttgtgaagcc
      541 tgcggcacta aaattgcctg cttcgatcag ttactatttg ttaaagaacg gttaggtgcg
      601 aaatcgtatg ctaatccgaa tttgcttctg gtaactcaac aacaatttag cgaagtaccc
      661 gactcgcacg tgaaagaccg cctgcgccgc gaagattacg ttaaaatggt ttgtcccaaa
      721 tgtcgccata aaatcgtcgt aaaagatgaa ttttaatgat ttaaagaaaa ctttagccgt
      781 ttatcatagc gaccgaataa tttttgtagg attaggtaat cgggattgcg gtgatgatgg
      841 cgcgagccag attttgctaa ataaattaaa gcacagtgaa gaatttaaag aagcgggatt
      901 catttttgca ggaactaatc cggaaaatta tttgcaaaaa atcactgaca aaaatccgca
      961 ggctgtggtc tttattgatg ccgcccattt tcaggaccag tccggaacgg taaaattcct
     1021 ggaagcaaag cagctgaaac cgtccgattt tagtacccac gccttttcga ttagcctaat
     1081 cgccgaatat ttgacttacg aaaaatcctg tgcgatttat tatctcggaa tccagccgga
     1141 aaatagcgga gttggtcaat cgctatccga atcggtccaa agcgggctag cgcatttttt
     1201 ctcagaaagt tagaaatata aatatggcta ccaatgaacc atataaatta attctgcctt
     1261 ttcatactct ggaaacgatg cagaccagaa tcagctggtt ggtaaacttg cgctggatag
     1321 gcataatcag tttattaggg ataattccga ttgccgaaca agttttaaaa ttcagtctgg
     1381 gatttccgca gatttatact ctggcggtgg tttttattgt tctcaatgtg acctattttt
     1441 ttatatgtaa gtatttcaaa tttgtcaatt tcgggcagga aatcgccttt accgaaatcc
     1501 agataatttt tgattttgtt ctgatttcgt ttgtggttca ttatacgggc ggtattgaca
     1561 atccgtttta tttcatttac ctcgtgcata ttatcatttc agggattctt tatcagggca
     1621 tctggcctta tatcaatgcc accgtagcgg cgtttttgtt gactttatgg tcgggactcg
     1681 aatatttcaa agtagtaaat gtctattcgt tgagcggttc accagctcct ataaaagtct
     1741 atttggtatc cttagccgcc ttctatattt tgatttatac ggctagctat ataatcaaag
     1801 atatcgtctc cggctaccga atgctgaaaa gagtgattga ccaaaaaagc gaattgctcg
     1861 aacaaactat gctggagcgc gataaaatgt ttcgtttcac agcgcatgag ctcaaatcac
     1921 cattgaccac tattcgcagt atgctggctg taattgatga ggtttattct gacagtctcc
     1981 aac
//
LOCUS       DAOMJU010000147         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_70347_length_1970_cov_22.334204, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000147 DAOMJU010000000
VERSION     DAOMJU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70347_length_1970_cov_22.334204"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLO58_09310"
     CDS             <1..461
                     /locus_tag="PLO58_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQO75022.1"
                     /translation="RDFANPDSTIIIDGERVNVSAWFDNPPTSFLVMMKNFFMGTDST
                     LGGMFPDRFKVGIAQENQALPTEFKLYPVYPNPFNPVANITFDLPQAADVKLCIVDLQ
                     GRVIEQIVNRRISAGRISYDWNASHYPSGIYFAMIEVNGHRSIRKMTLLK"
     gene            470..1936
                     /locus_tag="PLO58_09315"
     CDS             470..1936
                     /locus_tag="PLO58_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO75023.1"
                     /translation="MRLIHQFFVGKSHYLFPTFFLTVSLSNLAFASHWQASLYNLLAF
                     PKIPTVTLAELNSPSLPFIKARIGYDDIYRKKVDQQTTLTISEQTNTLQLNLPFRLRQ
                     TSHLIKAEAARNFSNFDNLSDQTSLVSHCQIDNTYFSTLWAMAYERIHFGGGLEYNRN
                     SAPFLINIISFPHSEDELTNKYFFDLLEPTFGEELTNTLHVNQIGYSLWTSLPLNSNY
                     HLGLAIRATDFDFHWRIRYINTGQEAALAGQRQIDLPINGLNHYYRLALSSDQGLVRD
                     ISVTFLRNDFDYFIDNNPPNFTDLDSLGQGYIDREGYAIQFTLGEKLFVISGGLSYAN
                     YESDIFLSTPVLGFQLGILPIFHSAELNFSYGHSFSQQIGFGTNFNWQNINFHAKALY
                     SHTLFDFWLEGKAKLMMGIKSDPLDYPYKYSLHLFDLNAEAEWRRGPLGIAYTFQQLI
                     PSGRRMDDSPIRLSKKVPGVDYTYRGGQQHRINLAYYF"
BASE COUNT          520 a          440 c          399 g          611 t
ORIGIN      
        1 atcgtgattt tgcgaacccg gattccacta ttatcattga cggcgagcga gtcaatgttt
       61 cagcctggtt cgataatccg ccgacaagtt tcctggttat gatgaagaat ttctttatgg
      121 gcaccgacag caccctgggc ggaatgtttc ccgaccggtt taaagtcggc atcgcgcagg
      181 aaaatcaagc tttaccgacc gaattcaagc tttatccagt ttatcctaat ccctttaatc
      241 cggtggcaaa tatcacattt gatttgccgc aagccgccga tgtcaaactg tgcattgttg
      301 atctgcaggg acgagtaatc gaacagatcg tcaatcgcag gatatcggct ggtcggattt
      361 cctacgactg gaacgcttcc cattatccat ccgggatcta ttttgctatg atagaagtca
      421 atggtcaccg gagcatccgc aaaatgactc tgctaaaata ggaccagagt tgcgactgat
      481 ccatcagttt tttgtgggga aaagccacta ccttttcccc actttttttt tgactgtcag
      541 tctgtccaat ttggcatttg ccagccattg gcaggcttca ctttacaatt tgctggcatt
      601 tcctaaaatt ccaaccgtaa cacttgccga actgaattca ccgagtttgc cttttattaa
      661 agcccgaatc ggttatgatg atatttatcg taaaaaggtt gatcaacaga cgactttgac
      721 catctctgaa cagacaaata ccttgcaact caatttgcct tttcgtctaa ggcaaacatc
      781 tcatctaatc aaagccgagg cggcaagaaa tttttctaat tttgataacc tcagcgatca
      841 gaccagtctg gtcagtcatt gtcaaattga taatacttat ttttcgactt tatgggcaat
      901 ggcttacgaa cgtattcact tcggtggcgg cttggaatat aaccggaact cagccccctt
      961 tttaattaat attatctcgt tcccacattc cgaagatgaa cttactaata aatacttttt
     1021 cgacttgctg gaaccgactt ttggagagga attgactaac accttgcacg ttaatcaaat
     1081 cggttattca ctatggactt cacttcctct gaatagcaat tatcatctcg gtttggcaat
     1141 tcgggcaact gattttgatt tccactggcg tattcgttat atcaacaccg gtcaagaggc
     1201 ggctttagcc ggtcaacggc aaattgacct tccaattaat ggattaaatc attattaccg
     1261 acttgctttg agttcagacc agggtttggt aagagatatt tctgtgactt ttttacgcaa
     1321 tgacttcgat tatttcatag ataacaatcc cccgaatttt actgatctgg attcgctcgg
     1381 gcaggggtat atcgatcgcg aaggctacgc gatccagttt accttgggtg aaaagctatt
     1441 cgtaatttca ggcggtctat cttatgccaa ttacgagtct gatatttttc taagtacacc
     1501 cgtactcgga ttccagttgg gaatattgcc gatctttcat tcggcggaat tgaatttttc
     1561 gtatggccat tcctttagtc aacagattgg ttttgggaca aatttcaact ggcagaatat
     1621 taactttcat gccaaggctt tatattctca tactttattc gatttctggt tagaagggaa
     1681 agccaaattg atgatgggta ttaaatcgga tccgctggat tatccataca aatatagtct
     1741 ccatttattc gatttaaatg ccgaagcaga atggcgtcga ggcccgctcg gaattgccta
     1801 tacttttcag cagctaatac cgtccggtcg cagaatggat gatagcccga tccgtttgtc
     1861 taaaaaagtg cccggcgtgg attacaccta ccgtgggggc caacagcacc ggatcaatct
     1921 cgcttattat ttttaatcat ccaaaaagca ggaattatat ttcttgctta
//
LOCUS       DAOMJU010000148         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_75902_length_1878_cov_22.312671, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000148 DAOMJU010000000
VERSION     DAOMJU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75902_length_1878_cov_22.312671"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /gene="pgi"
                     /locus_tag="PLO58_09320"
     CDS             complement(<1..1661)
                     /gene="pgi"
                     /locus_tag="PLO58_09320"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQO75024.1"
                     /translation="MLPSVNPTSTSAWQKLSAHYQEIKKISLRNLFNEDPERFQRFSI
                     NLVLDATSRDEILLDYSKNLITPETFRLLLKLARECGVAEAIELMFTGAAINVTENRA
                     VLHTALRNLDDNPIYVDGQNVMPPIKAVQEQMREFSAKVINGEWRGYDGQPIKDVVNI
                     GIGGSDLGPAMVTEALKPYHIPHLKLHFVSNVDGTDICETLKCLNPATTLFIIASKTF
                     TTQETMTNAQTARNWFLTTVGEPDFIKSHFVAVSTNRAKVIEFGIDPENMFVFWDWVG
                     GRYSLWSAIGLSITLAVGFDNFRELLAGAYAMDNHFRRTPFEKNIPVILALLGIWYNN
                     FFGAETLAILPYDQYLRRLPAYLQQADMESNGKRVDRNGKVVNYQTGPIVWGEPGTNG
                     QHAFYQLIHQGTKLIPCDFLAPAISHNPVGEHHNILLSNYFAQTEALMNGKSEEEVAR
                     ELKSKGLTESEVQRQLPYRVFPGNKPSNSILFKKLTPRTLGALIAMYEHKIFTQGIIW
                     NIYSFDQWGVELGKQLAGRILPELANDQPITSHDSSTNGLINAWK"
BASE COUNT          523 a          439 c          398 g          518 t
ORIGIN      
        1 ctcttccagg cattaatcaa tccattagtt gagctatcgt ggctggtaat cggctgatca
       61 ttcgcaagtt ccggcagaat ccgtccagcc agttgcttgc caagttcgac accccattga
      121 tcgaaactat aaatattcca gattattccc tgagtgaaaa tcttatgctc gtacatagca
      181 attagagcac ccagcgttcg cggagttaat tttttaaaaa ggattgaatt gctcggctta
      241 tttccaggaa aaacacgata gggcaactgg cgctgaactt cagactcagt caaacctttg
      301 gatttcaatt cccgggcaac ttcttcctcg cttttgccgt tcatcaaggc ttcggtctga
      361 gcgaaataat tcgataatag aatattatga tgttctccaa ccggattatg cgaaatggct
      421 ggcgctagaa aatcgcaggg aataagcttg gtcccttgat gaataagttg ataaaaagcg
      481 tgctgtccgt tcgtacctgg ttcgccccag acaattggcc cggtctgata gttgacgact
      541 tttccgttgc gatcaactcg cttgccgtta ctttccatat ccgcctgttg gagataggcc
      601 ggcagacgcc ttaaatattg atcataaggc aaaatagcca gcgtttccgc tccgaaaaaa
      661 ttgttatacc aaattcccag caatgccaga atcaccggaa tatttttttc aaaaggcgtc
      721 cggcgaaaat ggttatccat agcataggcg ccagccagca attccctaaa attatcaaac
      781 ccgaccgcca gcgtaattga taaaccgatt gctgaccaga gcgaatagcg accgccgacc
      841 caatcccaga acacgaacat attttccggg tcaattccaa attcgattac ttttgcccga
      901 ttagtcgaaa ccgctacaaa gtgcgatttg atgaaatcag gctcaccgac cgtggttaga
      961 aaccaattgc gcgccgtctg ggcattagtc atcgtttcct gcgtcgtaaa agtcttggag
     1021 gcaatgataa aaagcgtagt tgccgggttt agacatttca aagtttcaca gatgtcggtg
     1081 ccatctacgt tggaaacaaa atgcaatttt agatgcggaa tgtgataagg ttttagggct
     1141 tcggtcacca tcgccggtcc cagatcactg ccgccaattc caatattaac cacgtctttg
     1201 atcggctgac catcgtaacc acgccattcg ccattgataa ctttggcact gaattcacgc
     1261 atttgttcct ggacagcctt tattggcggc attacattct gaccatcgac gtaaatcgga
     1321 ttatcatcga gatttcgcaa tgccgtgtgg aggaccgcac gattttcggt gacattaatc
     1381 gccgccccgg tgaacatcaa ttcgatcgcc tcggcgacgc cgcattctcg cgccagtttg
     1441 agtaaaagtc gaaaagtttc aggtgtaatt agatttttac tataatcgag cagaatttcg
     1501 tctcgactcg tagcatcgag aactaaatta attgaaaaac gctgaaaccg ctccgggtct
     1561 tcattgaata aatttcgcag tgatattttt tttatttctt gataatgagc tgacagcttt
     1621 tgccaagcgg aagtggaagt cggattgact gaaggtaaca tttttatatt ctcctgtcat
     1681 taaacgccaa agattacaaa aaaaaattcc cgaatccaat aattcacccc aaaaattgaa
     1741 caattgtact cgtggaatta tcttacagtt gacgaatgca tttgattcag caatcgttcg
     1801 taaatccggt agttatcgcc gatgagaaca cagcgcacgg ttcgcggaaa ttcgttgttc
     1861 gctaaatgct gtaagatg
//
LOCUS       DAOMJU010000149         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_77049_length_1860_cov_15.439335, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000149 DAOMJU010000000
VERSION     DAOMJU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77049_length_1860_cov_15.439335"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1830
                     /locus_tag="PLO58_09325"
     CDS             4..1830
                     /locus_tag="PLO58_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQO75025.1"
                     /translation="MGSLFIYLSSFLIAPAQPSGGPYGPIHQTYELPSVKGKIYYVAP
                     DGKAESSGEALDKPTTLENAIEKVKTGDAIILRGGVYRTGGLLLNQGITMQPYKDEKP
                     ILKGTYIATDWKNLGNGLWKTTWTRLFPSQPDDWWVRDREGKKTPLHRFNNDMVFVDG
                     KFLQSVGWEGEVDENSYYINYDKGEIYIGIDPTNRLVEITAFNSAIVRTTGEVHSKKS
                     DGKGPTIRGITFTQYAYRAIEIEGKYPEGLSSEADHGKDVVGTTIENCTISYCSRVAG
                     YFKGDHMTFRHCKISDTSTEGLYIIASNDVLLEKNIFTRNNIENITGYFPAAVKIFNQ
                     CYRVTCNDNLVIDLPNSNGIWYDVGNVDGVFINNWVEGVGRNDGYFSKDRPWPSENGF
                     FFEISKGVTCAGNVFVNCDHGIWILNSSNAHIYNNTLVNSTACFGRNERSAEGDHFGW
                     HPSTGPDVDEREGHIFVNNLMYGDKDFERPLLFVWQPASLCERLSQPMLTQLDYNIYV
                     RADERKYPLILWSPAANPNCQIEIKSLAEIRQLYPKFSANSQEFLNYCNPLFKCPELH
                     NFQVIPEFSAVKLGFKLPADVKKLLKSSEKVIGYIGAYPPLP"
BASE COUNT          542 a          384 c          420 g          514 t
ORIGIN      
        1 gccatcggta gtttgtttat ttacttaagt tcatttctga tagcaccggc tcagccatcc
       61 ggtggtccgt atggaccgat tcaccaaacc tatgagttac cgagtgtaaa ggggaaaata
      121 tattacgttg cccctgatgg aaaagccgag tcgtcaggag aagcgcttga taagcctacc
      181 accttggaaa acgctattga aaaggtgaaa accggggacg ccataattct acgcggcggc
      241 gtctatcgca ccggcggttt actcctaaat caaggtatta cgatgcaacc ttataaagac
      301 gaaaagccga tattgaaggg aacgtacatt gcgaccgact ggaaaaattt agggaacgga
      361 ttgtggaaaa caacctggac tcgtctattt ccctcccagc cggacgattg gtgggttcgc
      421 gaccgcgaag gcaaaaagac tcccttgcat cgtttcaata atgatatggt ttttgtggac
      481 ggcaaatttc tccaatcggt cggctgggaa ggcgaagttg acgagaatag ctactacata
      541 aattacgata aaggagaaat ttacattggt atagacccaa ccaatcgtct ggtggaaata
      601 acagctttca acagcgccat tgtcagaacg acaggggaag tacacagcaa gaaatccgac
      661 ggtaaaggtc cgactatccg tggtataact tttactcagt atgcttatcg ggcaattgag
      721 atcgaaggta aatatccgga aggtttatcc tctgaagctg atcatggcaa ggatgtcgtt
      781 ggcactacca ttgagaactg cacgatttcc tattgttcac gtgtggctgg atatttcaaa
      841 ggcgatcata tgaccttccg ccattgtaaa attagcgaca ctagtaccga aggtctttat
      901 atcattgctt ccaatgatgt actactcgaa aagaacattt ttacgcgcaa taacatcgaa
      961 aatatcaccg gttattttcc ggcggcagtc aaaattttca atcaatgtta ccgggtgact
     1021 tgtaatgata atctggtgat tgatctcccg aattcaaatg gtatctggta tgatgtcggc
     1081 aatgttgacg gcgtattcat taataactgg gtcgaaggcg tcgggcgtaa tgacggctat
     1141 ttttccaaag atcgcccctg gccgagcgaa aacggcttct tctttgaaat atcaaaggga
     1201 gtaacctgtg ccggcaatgt ctttgtgaat tgtgaccatg gaatctggat tcttaatagt
     1261 tcgaacgccc atatttacaa caatacttta gtcaatagta ccgcctgttt cgggcgtaat
     1321 gaacgcagtg ccgaaggtga tcatttcggc tggcatccca gcactggacc ggatgtggat
     1381 gaaagggaag ggcatatctt cgtcaataat ttaatgtatg gtgataagga ttttgaacga
     1441 ccattgctat ttgtctggca accggcttcg ctgtgtgaac ggcttagcca gccgatgctg
     1501 actcaactcg attataatat ttacgtgcgg gctgatgaac gaaaatatcc cctgatttta
     1561 tggagcccgg ccgctaatcc taattgtcaa atagaaatca aatcattagc agaaatacgg
     1621 caactatacc caaaattttc agccaatagc caggaatttc tgaattattg caatcccctg
     1681 tttaaatgtc cggaattgca caattttcaa gtaataccag aattttcagc agttaaatta
     1741 ggttttaagc taccggcaga tgtcaaaaaa ttattaaaaa gttcagagaa ggttattggt
     1801 tatataggtg cgtatccacc cttaccataa tttcttctat aaaattttcc ttgacttttt
//
LOCUS       DAOMJU010000150         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_78503_length_1838_cov_19.992148, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000150 DAOMJU010000000
VERSION     DAOMJU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78503_length_1838_cov_19.992148"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLO58_09330"
     CDS             complement(<1..242)
                     /locus_tag="PLO58_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HQO75026.1"
                     /translation="MNKIHPTAIIDSQARLGENISIGPYSIIGNNVEIGDGCQIGSHV
                     LILPGARIGRDCKIYHHAIISEIPQDLKFAGEETTA"
     gene            complement(246..1643)
                     /locus_tag="PLO58_09335"
     CDS             complement(246..1643)
                     /locus_tag="PLO58_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HQO75027.1"
                     /translation="MSRHQRTIKNEVSISGIGLHTGVKSTITFKPAPENSWIKFVRTD
                     VENCPEIVADINHVTDISRGTTLSQNGVQVHTVEHVLSAVAGLGIDNIIIAINNIEPP
                     VCDGSAIEFVRALKRAEIVEQEAEQDILVIDKILTYSDPKKHVDIHITPAEKFQITFL
                     IDYSEIPAIGTQYSTTYSLEDEFETDYAAARTFCTLSEIEELKNRGLIKGGNVNNAVV
                     FVDRHIEASEVERLRELFGISSDISIGQGGTLNGVELRYYNEAVRHKIVDLIGDFALL
                     GVPIQGHVIAARSGHAHNINFVRKIRKEYEKVMISKKYQKGKVKGALFDISGIERILP
                     HRFPFLMVDKIVDMVPGESVTAIKNVTINEPFFQGHFPVKRVMPGVLVLECLAQAGGF
                     MLLNTIENPEKVLLYFTGLDEVRFRKPVVPGDQIRLEVKMVQSRLSMCKMRGVAYVND
                     EVAVEANMMASLVEL"
     gene            complement(1647..>1838)
                     /locus_tag="PLO58_09340"
     CDS             complement(1647..>1838)
                     /locus_tag="PLO58_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQO75028.1"
                     /translation="TGVMIAAQSGVTKDVPAGTFLFGSPAQERLKAAQELAHIRSLPE
                     IKERLKKVEMELRKGKNIG"
BASE COUNT          529 a          436 c          350 g          523 t
ORIGIN      
        1 tcggcagtgg tttcctcgcc agcaaattta agatcctgag gaatttcgga aattatggca
       61 tgatgataga ttttacagtc acgaccgatt cgagcgccgg gtaaaattaa tacatgactt
      121 ccaatctgac agccgtcacc aatttcaaca ttgttgccga tgatactgta gggaccaatt
      181 gaaatatttt caccaagtcg agcttgggaa tcaataatag cagtaggatg aattttattc
      241 atattctata attccactag cgaagccatc atatttgcct caacagcgac ttcatcattg
      301 acataagcga cgcctcgcat tttacacatt gataaacggg attgaaccat tttaacctca
      361 aggcgaatct ggtcgcccgg cactaccggt ttccggaatc tgacctcatc caatccggta
      421 aaatacaata aaaccttttc gggattttca atcgtattca aaagcataaa tccgccagcc
      481 tgcgccaggc attccagaac caaaaccccc ggcatgaccc gtttgaccgg aaagtgccct
      541 tggaaaaacg gctcattaat tgtaacgttt ttaattgcag tgaccgattc accgggaacc
      601 atatcaacaa ttttatctac cattaagaat ggaaaacgat gcggaagaat acgctcgatg
      661 ccgctaatat caaataaagc ccctttaact ttgcccttct ggtatttttt actaatcata
      721 accttttcgt attccttgcg tattttccgg acaaagttaa tgttatgggc gtgaccactt
      781 cgcgcggcga taacgtgtcc ctgaatcgga acacccagca gggcaaaatc gccaattaaa
      841 tcaacgattt tatgccgcac ggcttcgtta taataacgca attctacccc gtttagcgta
      901 ccgccttgac caatggaaat atcactggaa atcccaaata attcacgaag ccgttcaact
      961 tcactcgcct caatatgccg gtctacaaaa accacggcgt tattgacatt accgccttta
     1021 attaaaccgc gatttttaag ttcttcaatt tcactcaaag tacaaaaagt gcgagcggcg
     1081 gcataatcag tctcaaattc gtcttcgagc gaataagtgg tcgaatactg cgtgccgata
     1141 gccggaattt cactgtagtc aattaaaaag gtaatctgaa atttttcagc gggtgtgatg
     1201 tgaatatcaa catgtttttt cggatcagag taggtcagaa tcttatctat gaccagaata
     1261 tcttgctccg cttcttgttc tacaatttca gcccgtttca aagctcgcac aaattctata
     1321 gcgctcccat cacagaccgg tggctcgata ttatttattg caattatgat attatcaatt
     1381 cccagaccag ctacagccga taaaacgtgc tcaacggtgt gaacttgaac cccgttctgc
     1441 gagagagtcg tgccgcgcga aatatcggtt acatgattaa tatccgccac aatttcggga
     1501 caattttcga catcagttcg tacaaattta atccaggaat tctcaggcgc cggtttaaaa
     1561 gtaatagtac tctttacgcc agtatggagt ccaataccgg aaatcgagac ttcattttta
     1621 atagttcgct gatggcgtga cataacttat cctatatttt ttccttttcg taattccatt
     1681 tctactttct tgagacgctc ttttatttcg ggtaaagaac gaatatgggc taactcctgg
     1741 gcggctttca aacgttcctg agctggagaa ccgaatagaa atgtcccggc tgggacatct
     1801 ttggtaacac ccgattgagc cgcaatcata actcccgt
//
LOCUS       DAOMJU010000151         1798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_81326_length_1798_cov_16.116466, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000151 DAOMJU010000000
VERSION     DAOMJU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81326_length_1798_cov_16.116466"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..952
                     /locus_tag="PLO58_09345"
     CDS             41..952
                     /locus_tag="PLO58_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO75029.1"
                     /translation="MKKKSKKYNPLRMEKELSDLIEYIENAGFESKEEIDRFLAKMGG
                     KKIGSVLNKKKTAADEAFELILEAQEVSPEEGIKLAKKAITLDPNCADAYTFIADHTE
                     DEDEALELYQKAMQVARESLGENAFIEYVGHFWGITKTRPYMRAKAGYASSLILKGDL
                     QSAIKEFQEMLVLNPSDNQGIRFSLSVLLLQLNRLREYEDLLKQYKDENSAGWLYNRV
                     LYLYLKYGGTTRTISALEEAIAENKYVMDLITGLEEPPKELPESYGIGSYEEAVLYYW
                     ESILTWMAHKKAILWLIRYWERRREIN"
BASE COUNT          610 a          334 c          364 g          490 t
ORIGIN      
        1 ttaaattaag ttatcgttgg attttagaag aggttatcaa atgaaaaaga aaagtaaaaa
       61 atacaaccct ttacggatgg aaaaggaatt gtctgatcta attgagtata ttgagaatgc
      121 tgggtttgaa tcgaaggaag agatagaccg ctttttagcg aaaatgggag gtaaaaaaat
      181 tggttcagtt ctaaataaaa agaaaacagc cgcagacgaa gcatttgaat tgattctcga
      241 agcccaagaa gtatcgcctg aggaaggaat taagctggca aaaaaggcta taactttgga
      301 tccgaattgt gccgatgctt atacttttat agccgatcat actgaggatg aagatgaagc
      361 cctagaatta tatcaaaaag cgatgcaagt tgcgcgtgaa tccttaggag agaacgcctt
      421 tattgaatat gtcgggcatt tttggggaat taccaaaacc cgtccatata tgcgggctaa
      481 agccggttat gcgtcttccc taattttaaa aggtgatctt caatcagcta tcaaggaatt
      541 ccaggaaatg ttagtcttga atccctccga caatcagggg attcgatttt cactatcagt
      601 gctgttgctc caattgaacc ggcttcgtga gtatgaagat ttattgaaac aatataaaga
      661 tgaaaatagc gctggctggc tatataaccg cgtcctctat ttatatttaa aatatggagg
      721 cactaccaga acgatctcag ctctggaaga ggctatcgcc gagaataaat atgtaatgga
      781 tttgatcacc ggtctcgaag aaccgccgaa agaattaccg gaaagctatg gcatcggtag
      841 ttacgaagaa gccgtcttat attactggga atctatttta acttggatgg cgcataaaaa
      901 agccatttta tggcttatcc gttactggga gcgacgccgg gaaatcaatt gaattaagtc
      961 ctatcgaatt tctctaaaaa tagcagaata acgttaaaaa acattcagga atatttttcg
     1021 gatttggtga gtcgctgaag gtttaacaat aaatcaaaaa gtcaaaatca gcctttaaat
     1081 acttggttgc gaaaccagat tgatattccc cagaaaaggg caattccacc agcaagaagg
     1141 acaaccaagt ctagaacaca tagtattcgg agacagctta tattgaaaat tttgggctca
     1201 ccattgactt gctgtaccaa taagtaaagg caaatcagag aaaccgaaag gacaattaac
     1261 cctcccccaa ctaaaacccg cgaccggtgc cgcaaccgcc ttggtaatat ttttgccata
     1321 cactccctcg ctaaaaacct tacctaccgt taattaaaac taatacttta tatcttaagg
     1381 agaatctgga gggaaaacaa tgaaaaattt aatggattaa gataatccta aaacaattag
     1441 ccggctttaa tccttagcca gagtaaaagc ccattcatca gcatgaaaaa cccgactata
     1501 caccaaagaa tcgccgccag cgctacactt cgtacaatcc aaagaccgag gtaggtaatt
     1561 aatgcttggt gtgccagcga aataataagt acgaaaacga ttggtaggaa aaaattaatt
     1621 aaaccacttc tcagagatct tttcagcttt tcccggttat cgcggtggtc ggagtggtgt
     1681 tttctgcggt gtcgtttagt gtgctccata ttatctcttg ttattgccca ttataaaata
     1741 aaaaaaatgt tctatttggt caagctaaag ttttttatcc tgcttaattt ataaatcc
//
LOCUS       DAOMJU010000152         1758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_84272_length_1758_cov_17.591897, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000152 DAOMJU010000000
VERSION     DAOMJU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84272_length_1758_cov_17.591897"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /gene="pgeF"
                     /locus_tag="PLO58_09350"
     CDS             <1..662
                     /gene="pgeF"
                     /locus_tag="PLO58_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HQO75030.1"
                     /translation="FRSLNLGIADGDTLKNILINRKRFYNTLNFTPSDIAQAEQVHSN
                     KVAIVEDNGPIQGCDALITTRTNLYLSVKTADCAAVLLYDPNHQVIAAVHAGWRGTAE
                     GIIANTINTMQARFQTNPGELIAAIGPALHVCCYKVKNNVAKKFSDNEIIQREEGLFL
                     DLILAITNRLNAAGVQLDRIADSGYCTACQPEKFYSYRRDEGVTGRMLAVIGINQRNK
                     C"
     gene            676..1254
                     /locus_tag="PLO58_09355"
     CDS             676..1254
                     /locus_tag="PLO58_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQO75031.1"
                     /translation="MFNLIVAVIIGVCIFFGLRNGLIKSAFKLIGLVLAALSASRYYY
                     FGGKIIADLFNLSEGVQAVIGFVIVFIVVFLLFELLASMLRSLLRALKLIWVDRIGGM
                     LFGFLEGAIIMIGIVWVLNVYPELGFVDHLRQSSANFKFFERSGEKVIKICELNSEMN
                     NLRKSMRKIVFLPETPQTNPENTNISPSENNP"
     gene            1259..>1758
                     /locus_tag="PLO58_09360"
     CDS             1259..>1758
                     /locus_tag="PLO58_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO75032.1"
                     /translation="MTIPESMKETNDKLGFAKIIELIQEMALSTAGKSLANTIKPLKD
                     IEIIRCRQSRINALQAILAAGDSLPLNSFSDSRAELDRCRVIGTFLSLETLIQIDQLL
                     ELVERQRRFFRSRRPQLLSLEELFEGLDSLPQVRKTIAAIIAPDGSIRDSASPQLNRI
                     RHEIRT"
BASE COUNT          466 a          356 c          400 g          536 t
ORIGIN      
        1 ctttccgttc acttaatctt ggaatcgccg acggcgatac tcttaagaat atcctaatta
       61 atcgaaaaag attttataat accctcaatt ttactccgtc cgatattgct caagccgaac
      121 aagttcatag caataaggtc gctatagtgg aagacaatgg tcctattcaa ggttgcgatg
      181 ctctaattac cactcgaacc aatttatatc tgagtgttaa aacggctgat tgtgcggcgg
      241 ttttattgta tgatccgaac caccaggtca ttgcggcggt gcatgccggt tggcggggca
      301 cagccgaggg gataatcgcc aacaccatta atactatgca ggctcgattt caaactaatc
      361 cgggcgaatt gatagccgcc atcggtccgg ctttgcatgt ctgttgttat aaagtgaaaa
      421 ataatgtcgc caaaaagttt agcgataatg aaataatcca gcgggaagag ggtctttttc
      481 tcgatttaat cctggcaata accaatcgct tgaacgcggc tggtgttcaa ctcgatagga
      541 ttgccgatag tggttactgt accgcttgtc aacctgaaaa attttattcc taccggcggg
      601 atgagggcgt taccggacgg atgctggctg taattggcat taatcagcga aataaatgct
      661 aagtgataga ttcggatgtt taatttaata gtagccgtaa ttattggagt ttgcattttt
      721 ttcggattac gcaatggctt gataaaatcg gcttttaagc ttattggctt agtattggcg
      781 gcgctctctg cttcacgata ttattatttt ggtgggaaaa taattgccga cctttttaat
      841 ctatccgaag gtgtgcaggc ggtgattggg tttgttatcg tattcatcgt ggtgttttta
      901 ctttttgaat tgctggcttc aatgttgaga agccttttac gagctttgaa attgatatgg
      961 gttgaccgaa ttggcggaat gctgttcgga ttcctggaag gtgccattat tatgatcgga
     1021 atagtctggg tattaaatgt ctatccggaa ctcggattcg tggaccatct gcgtcaatct
     1081 tcagccaatt ttaagttttt tgagcgaagt ggtgagaaag ttatcaaaat ttgcgaactg
     1141 aattcggaga tgaataatct ccgtaaaagt atgcgtaaaa tcgttttctt gccggaaacc
     1201 ccgcagacaa atccagaaaa cacgaatatt tctccaagtg aaaataatcc ctgaattaat
     1261 gacaatacct gaatctatga aggaaaccaa cgataaactc ggttttgcta aaatcatcga
     1321 gttgattcag gaaatggctc tctctactgc cggtaaatct ctggctaata ctattaagcc
     1381 gctaaaagat attgaaatta ttcgatgccg gcaaagtcgc attaatgcgc tccaggctat
     1441 tctggccgct ggtgattcgc taccgttgaa ttctttttcc gattcccgcg ccgaacttga
     1501 ccgctgtcgt gtcatcggaa catttttatc ccttgaaact ctgattcaga ttgatcaatt
     1561 actggaattg gtggagcgcc aacgtcggtt ttttcgctct cggcgtccgc aactattgag
     1621 tttggaagaa ctatttgaag gtttggattc tctgccccaa gtacgtaaaa ctattgcggc
     1681 tatcatcgcg ccggacggtt cgattcgcga tagcgccagt ccgcagctta atcgaattcg
     1741 ccatgaaatt cgcactca
//
LOCUS       DAOMJU010000153         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_86686_length_1727_cov_16.618421, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000153 DAOMJU010000000
VERSION     DAOMJU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86686_length_1727_cov_16.618421"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="PLO58_09365"
     CDS             complement(<1..1706)
                     /locus_tag="PLO58_09365"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HQO75033.1"
                     /translation="MKNGQPNNGIVELGNDGQPTGVVYNLNWLNPGPDYPYSWAEGPV
                     MWKYNGYYYYSFARDLGGGQKVMRSRTLTADQSAWEMLGDFFNENDPQKGSSLFTSPN
                     HASAAVMLDDSTSWVIHPLYAKGEWKGQGRQGLLNQVHYDANGKPVADYPVNKSFTAP
                     KLPSSGIPWMVPHSDFFTSSELHPEWSFLGYTPAVKYSLTDRPGWLRLSPKDKALANT
                     VIKNDGEHNYSLITRLDFNAQSTTDQAGLWIMRGDQTMEVKLVSTLSTEGFKVITYSF
                     KSTIYETENTIGDTLWLKLVRINHKISGYFSGDGFDWIEIGAPIDISEIDSYSDFSTF
                     TGTRQGLYVKGMSDAWFDFYIYRDAYTPIMAECPANQFGTALVLNKYPPNSLGKIHND
                     DWALYAGVEFGNTEYAKSCDSLKITASCLTAGSAVEVYLDSIDVNTKIAVCAIDTTGS
                     WTNFQTFTTDLLNPISGNHDVYLKFVGPGSGELFRLQSFQFITNSKPTAINSRPENNL
                     PRRFYLEQNYPNPFNPVTRIKYSVPKTSYLTIKVYDVMGREVATVFDGVQQAGNYTAI
                     LDG"
BASE COUNT          472 a          392 c          381 g          482 t
ORIGIN      
        1 ccatccaaaa tggcggtata atttcccgcc tgctgaacgc catcgaatac cgtagctacc
       61 tcccgcccca tcacatcata aaccttgatg gtcaggtaac tggttttcgg cactgaatat
      121 tttattcgag ttaccggatt gaaaggatta ggataattct gttcaagata aaatcttcgc
      181 ggtagattat tttccgggcg actattgatt gcggtgggtt tggaattggt aataaattgg
      241 aacgattgga gtcgaaataa ttccccactt ccaggaccaa cgaattttaa atatacgtca
      301 tgatttccag atatcgggtt caataaatct gtggtaaaag tctgaaaatt tgtccatgag
      361 ccggtcgtgt caatggcgca aaccgcaatt ttagtattta catctatcga atcaaggtaa
      421 acttccaccg cgcttccggc agtaaggcat gaagcggtta tttttagcga atcacaactt
      481 ttcgcatatt cagtattgcc gaactcaacc ccggcgtaca atgcccaatc atcgttgtgg
      541 attttgccca gtgaatttgg cgggtattta ttcaatacta acgcagtgcc aaactgattc
      601 gccgggcatt cagccattat cggcgtataa gcatcgcgat aaatataaaa atcgaaccag
      661 gcatcgctca tccctttcac gtacaggcct tggcgcgtac cggtaaaggt cgagaaatcg
      721 gagtaggaat ctatttccga aatatctata ggggcgccta tttcgatcca atcgaatcca
      781 tcgccgctaa aatatccgct gattttatga tttatacgaa ccaattttaa ccagagagta
      841 tcgccgatgg tgttttcagt ttcgtatatg gtagatttga agctataggt tatcaccttg
      901 aagccttcag tcgataaggt gctaacaagt ttgacttcca tcgtttgatc acctctcata
      961 atccagagac cggcttggtc ggtagttgat tgagcattaa aatccagtcg cgtaataagt
     1021 gaataattat gttcgccgtc gtttttgatt accgtgttag ctagcgcttt atctttgggc
     1081 gataaacgca accagccggg acgatcggtg agagaatatt taacggcagg tgtataaccc
     1141 aaaaatgacc attccggatg taactcagac gaggtaaaga aatccgagtg aggcaccatc
     1201 cagggaatac cgctactcgg caatttcggc gcagtaaacg atttattaac cgggtaatca
     1261 gccactggtt tgccattcgc atcataatga acttgattta acaatccctg ccgtccctga
     1321 cctttccatt ctcccttggc atagagtgga tggataaccc acgaagtact gtcatcgagc
     1381 ataacagccg ctgaagcatg attgggactg gtgaaaagtg aggagccctt ttggggatcg
     1441 ttttcattga aaaaatcgcc caacatttcc caggcggact gatcggcagt cagagtgcga
     1501 ctgcgcataa ctttttgacc tccgccaagg tcacgggcaa atgaatagta atagtagccg
     1561 ttatatttcc acatcaccgg accttccgcc caactgtaag gatagtccgg gcctggattt
     1621 aaccaattga gattatagac gacgccagtc ggttgtccgt cattgccgag ctcaacgatg
     1681 ccgttattgg gttgtccatt tttcactact aaataccatt tatggtc
//
LOCUS       DAOMJU010000154         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_86767_length_1726_cov_22.183722, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000154 DAOMJU010000000
VERSION     DAOMJU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86767_length_1726_cov_22.183722"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLO58_09370"
     CDS             <1..228
                     /locus_tag="PLO58_09370"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HQO75034.1"
                     /translation="VETYGKVRLTVYNLLGQTVRNLVRENQYAGAYLVTWDGRNNNGK
                     QVASGIYYLKMEVLNGDKVLFQSSKKLMLVK"
     gene            238..1164
                     /locus_tag="PLO58_09375"
     CDS             238..1164
                     /locus_tag="PLO58_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HQO75035.1"
                     /translation="MRKRVILNFFVCGLITAGFLSRCEKIIYTYDDQKPQITTPLTIL
                     AVTDSSAEIYWETDEACHIKIKYGLTTDYGLEYEDPENCEIHLATLRGLEPHALYHLQ
                     VYCWDFAANGPVKSPDTTFCTLANESSYLREGWQFFREKNYSAAKTAFDSAHQINPFL
                     AEIQAAYGWLQIRTNDLDSARQNLTAAYNLDHYLPITLAGLAVISMIDDVPLAAINYV
                     KAVLSLDQQWEYRYNPEISWKSLLLIRAQAYFQTNQMNLAQADLDSLWPQNGLNPDLP
                     SSWVVNKQSYDTYESAFGAALNYLIANPEKMG"
     gene            complement(1180..1404)
                     /locus_tag="PLO58_09380"
     CDS             complement(1180..1404)
                     /locus_tag="PLO58_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HQO75036.1"
                     /translation="MASEKETEDKNISRRNFLKKLAATAVFTVPTIQSFSLLQTQPNG
                     GWWWWTGHHHHHAPGPGEPTPPLPPPPPGL"
     gene            1510..>1726
                     /locus_tag="PLO58_09385"
     CDS             1510..>1726
                     /locus_tag="PLO58_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO75037.1"
                     /translation="MTITQKIILTISLAFVTLSLINCEKEVIKVIDDLDPPVILTEPN
                     VEVAANSVTIFWTTDEPCSVSVKYFVIG"
BASE COUNT          489 a          377 c          398 g          462 t
ORIGIN      
        1 gtcgagactt acggcaaggt gcgcctgact gtttacaatc tgctgggtca aaccgttcgg
       61 aatctggtac gggaaaatca atatgcggga gcttatttag tcacctggga cggtcgtaat
      121 aataacggca aacaggttgc ttccggcatt tattatttga aaatggaagt cctcaacggc
      181 gataaggtct tgttccaaag cagtaaaaaa ttgatgttgg ttaagtaagg cgggaaaatg
      241 aggaaacgcg tcatcctaaa ttttttcgtt tgtggcttaa tcactgccgg atttttatcc
      301 cgatgcgaga aaattatcta tacctacgat gaccaaaaac cgcaaatcac cacgccgctg
      361 accattttgg cagtaaccga cagttccgct gagatttact gggagaccga cgaagcctgc
      421 catattaaaa taaaatatgg cttgacgacc gattatggtc tcgaatacga agacccggaa
      481 aattgcgaaa ttcacctagc tactctaagg ggcttggaac cgcatgctct ctatcatttg
      541 caggtttatt gctgggattt cgccgccaat ggcccagtaa aatcgcccga taccactttt
      601 tgcactctgg ctaatgagtc cagctatctg cgcgaaggct ggcagttttt cagagagaaa
      661 aattactccg ccgctaagac tgcttttgat tccgcccacc agataaatcc atttttagct
      721 gaaattcaag cggcttatgg atggctacaa atcagaacga atgatttgga ttccgcccgc
      781 cagaatttga ccgccgccta taatctcgac cactatttgc cgattactct ggcgggattg
      841 gcagtaatca gtatgattga cgatgtgccg cttgccgcga ttaattatgt aaaagcggtg
      901 ttaagccttg atcagcagtg ggaatatcgt tataacccgg aaatcagctg gaaaagcctg
      961 ctcttgattc gggcgcaggc ttactttcag acgaatcaaa tgaacctcgc tcaagctgat
     1021 ctcgattccc tctggccgca aaacggattg aatcccgact tgccgtccag ctgggtggtc
     1081 aataaacaat cttatgatac ttatgaaagc gcctttggag cggcattaaa ttacctgata
     1141 gctaatccgg agaaaatggg ataatgttcg gtgaaaaatt tacaaacccg ggggcggtgg
     1201 gggcaaaggc ggagtcggtt caccaggacc gggggcatgg tgatgatgat gaccggtcca
     1261 ccaccaccag cctccattcg gttgagtctg taaaagcgag aaagattgaa tggtcggaac
     1321 cgtaaaaacc gccgtagccg ccagtttttt caagaaattg cgccgactga tatttttatc
     1381 ttcagtttct ttttcgcttg ccatattaag taccttgtta atattgaaaa aactaatcat
     1441 aatataaagt gattaattgc tataatcaat agagaataat tcgatcggta gaattcacgg
     1501 agtcttgtga tgaccattac tcaaaaaata atcttaacca tctcactggc gtttgtcacc
     1561 cttagtctga ttaattgcga aaaagaagtt atcaaagtca tagacgatct ggaccctccg
     1621 gtaattctga ccgaacccaa cgtggaagtc gcggctaata gcgtgacgat cttttggacg
     1681 accgacgaac cgtgttcagt ttcggttaaa tattttgtaa ttggaa
//
LOCUS       DAOMJU010000155         1681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_90348_length_1681_cov_15.402214, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000155 DAOMJU010000000
VERSION     DAOMJU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90348_length_1681_cov_15.402214"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1670
                     /locus_tag="PLO58_09390"
     CDS             120..1670
                     /locus_tag="PLO58_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HQO75038.1"
                     /translation="MIKKRILILSLFVMTLVTMALPQYYISPYEKVYDDLLYLQAGGF
                     LRDLDLNHTPISTIQIAQSLKLQLKSDNYTTAGATQRLLIDRIAVTYAPADTDLVLNL
                     KRKLSDLLKIESGQSYLHYGVKINLDYQNQPQSKLYPQLRTFGSLSVTKNLSITNVMT
                     LDPFATENPDYVGKEWRGLSGYTEQAYLMWSSRAFRLTAGRSYIVFGPGRKSSLLFSS
                     AARPLDNLKFDIFLKHLSFQGITAKLDKMDENERYLSAHRLSFYLGNWAIGLTEAMLY
                     TGINQNLEFAYLNPFLLYHGEQLNGPDLNGNTLGTLELSYTGRNWIAYSEFLVDDIQF
                     DKEEPGDLEPNEVGCILGMDIADPFKIPGVYLGMEYTAITNRTYKTPTRSEWFLHRNV
                     PIGYELGSDLDRFNVLVRKYHKNLQFALEFDHIRQGEGDLNKPWDSPWDTLTVEQGYS
                     EKFPTGKVQTTSDVGLEVTWMPDYGKYIYARLTYEAIKNIDHGNKNAQNLIFSVGAYL
                     DLHGKIGF"
BASE COUNT          475 a          343 c          358 g          505 t
ORIGIN      
        1 ctttcgtatt taataaattc agcttgcgaa acagcgcttg attttcctct ttggtctttc
       61 gctttcaggc gttaatatat gtcgtgtttc cgataaattt gatggaaggc agaaaaaaga
      121 tgataaaaaa aagaatttta atcttatcgc tatttgtaat gactttagtg actatggcat
      181 tgccacaata ttatatttcg ccttatgaga aggtttatga tgatctactc tatttacaag
      241 ccggtgggtt cttacgagat ctcgatttga atcatactcc gatttcgacc attcaaattg
      301 cgcaatcctt aaaacttcag ttgaaatcag acaattatac cacagccgga gcgactcaac
      361 gtttgttgat tgataggatt gcggtgactt atgctcccgc tgatactgac cttgttttga
      421 atttaaagcg caagctctct gacttattaa agattgaaag cggtcaatca tatttacact
      481 atggggttaa aattaacctc gactatcaga atcaacctca gtccaaactt tatccccagc
      541 tgcggacttt tggttctctt tccgtaacca aaaatctcag tattaccaat gttatgacac
      601 ttgatccctt tgcgaccgag aatccggact atgtcggaaa ggaatggcgc ggtctatccg
      661 gttatacgga gcaagcttat ttgatgtgga gttccagagc attccgtctg actgccgggc
      721 gtagttatat agttttcgga ccgggacgca agagctcatt actattttct tcggcggcgc
      781 gcccattaga taatctaaaa ttcgatattt ttctgaaaca ccttagtttt caaggaatca
      841 cagccaaact tgataagatg gatgaaaacg agcgctacct ttcagcgcac cgcctaagtt
      901 tctatctggg aaattgggcg atcggcttga cggaagcgat gctatatacc ggtattaacc
      961 agaaccttga attcgcttat ctgaatcctt ttctacttta tcacggtgaa caattaaatg
     1021 gtcctgacct aaatggcaat accctcggga ccctggaact cagttatacc ggtcggaact
     1081 ggattgctta cagtgaattt ttagttgatg acattcaatt cgataaagaa gaacccggtg
     1141 atcttgaacc gaatgaagtc ggttgtattc tggggatgga tattgctgat ccgtttaaaa
     1201 ttccgggcgt atatctcgga atggaatata cagccattac caatcgcacc tacaaaacac
     1261 caacacgaag cgagtggttt ttacaccgca atgtgccgat tggttacgag ctgggcagtg
     1321 acttagaccg ttttaatgtg ctcgttcgta agtatcataa gaacttgcaa tttgcattag
     1381 agttcgacca tattcggcag ggtgaaggtg atttgaataa accctgggat tcgccctggg
     1441 ataccctgac tgtcgaacaa ggttactcgg agaaatttcc aaccggtaaa gttcaaacga
     1501 ccagtgacgt aggactggag gttacctgga tgccggatta cggtaaatac atttatgcac
     1561 gtctaaccta cgaagcgatc aaaaatatag atcatggtaa taaaaacgcg cagaatttga
     1621 ttttttccgt tggagcctac ttggatttac atggtaagat tggtttttaa tttcccgcga
     1681 a
//
LOCUS       DAOMJU010000156         1680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_90431_length_1680_cov_13.032615, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000156 DAOMJU010000000
VERSION     DAOMJU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90431_length_1680_cov_13.032615"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /gene="htpG"
                     /locus_tag="PLO58_09395"
     CDS             <1..1268
                     /gene="htpG"
                     /locus_tag="PLO58_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQO75039.1"
                     /translation="IIQKYSNFINFPIYVGKERVNSIEALWRKPKDEIKEDELIEFYK
                     FLTNDINPPLGHLHLSLEGSVNFKALLFVPSSTPYDIFYEIREKSLHLYANRVFVQDN
                     ATDLLPEYLRFIKGVVDTEDLPLNISRQVTQDSPVMAKIRNVLTTRILAMLEDWAKND
                     VSKYEKFYKNFGHIFKLGVTTDFANKDKIIKLLRFETTAKNKGELTSLDEYIARMPKD
                     QKEIYYLTGENREIAERNPKLEYFRKHNLEVLLLVDPIDIFIIPTIYEYEKKKFQSIE
                     KADLKLEKKAQSAEGEALEENLLKSLIAVFKETLGDKVEDVIVSNRLVDSPATLVVGK
                     EGLEPQIEKFMKMMNQEVKPQKKILEINPAHPLIKNLSRLNLGNSHDPLLRQSILQIY
                     ESALLLDGNLENPTEFVARMVDILTTATK"
BASE COUNT          534 a          318 c          344 g          484 t
ORIGIN      
        1 ccattatcca gaaatactcc aatttcatca acttcccgat ttatgttggc aaggagcgag
       61 tgaacagcat cgaagcgctc tggcgtaagc ctaaagatga aataaaagaa gacgaactga
      121 tcgaatttta taaatttctt acaaacgata ttaacccgcc actgggacat ctacatttgt
      181 cgctggaagg ctcggttaac ttcaaagcct tgctgttcgt gccgtccagt acgccttatg
      241 acatttttta tgaaattcgc gaaaaatcct tgcatctcta tgccaatcgt gttttcgtac
      301 aggataacgc cactgattta ttgccggaat atttacgatt tatcaaaggt gtggttgata
      361 ctgaagacct accgttaaat atttcccgcc aagtgacgca agacagcccg gtaatggcca
      421 aaatccgtaa tgtattgact actcgcattc tagcaatgtt ggaagactgg gcaaagaatg
      481 atgtcagcaa atatgaaaaa ttttataaga atttcggtca tatttttaaa ctaggcgtta
      541 ccaccgattt tgctaataaa gataaaatca tcaaactctt gcgttttgag actaccgcga
      601 aaaataaggg cgaattgacc tcgctcgatg aatacattgc gcgtatgccg aaagaccaaa
      661 aggaaattta ctacctgacg ggcgagaacc gcgagattgc cgaacgcaat cccaaactgg
      721 agtacttccg gaaacacaat cttgaagttc ttttgctggt tgatccgatt gacattttca
      781 tcataccgac gatttacgaa tatgagaaga aaaaatttca gtcaatcgaa aaagccgacc
      841 tgaaattgga aaagaaagct caatcggcgg aaggtgaagc tcttgaagag aacttattaa
      901 agtcactgat agcggttttt aaggaaacgc tgggtgataa agtcgaggat gtaatcgttt
      961 cgaatcgttt agtggattca ccggcaactc tggtcgttgg taaagaagga ctcgagcctc
     1021 agatcgagaa atttatgaaa atgatgaacc aggaagtcaa accgcagaag aaaatccttg
     1081 agattaatcc ggctcatcct ttgattaaaa atctctcccg tttgaacctc ggcaatagtc
     1141 atgatccgct actgcgtcaa tcaattctac agatttacga atcggctttg ttattagacg
     1201 gtaatttgga aaatccgact gaatttgttg cgcgtatggt tgatatttta actactgcaa
     1261 ccaaataatc ataatcgtag gacaggcatt cttgcctgtc cacaataaat tgtaggtcga
     1321 aataatattt cgatcattgt gatttaacaa atcgctttac aattttcttg atggttatat
     1381 aacgacagcc tggaaaggct gtcctacgta ttagaatcgt aggacagata ttcttgcctg
     1441 tctattaaat tgttatagct ctttattaga aggtatgaga aagagttgac agcctagaaa
     1501 gactgtccta cgtatataca tagctgctaa aattaattac cgctggcgca agatttttgt
     1561 tggcaacttt aaatttagtc tttagtttaa acctgatgtc tataattatt aagaaagaga
     1621 aaattatttg ttcgtagatt acaccaaagt atctgtgcaa tccggtagcg gtggtaaggg
//
LOCUS       DAOMJU010000157         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_97135_length_1605_cov_18.272258, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000157 DAOMJU010000000
VERSION     DAOMJU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97135_length_1605_cov_18.272258"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLO58_09400"
     CDS             <1..639
                     /locus_tag="PLO58_09400"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HQO75040.1"
                     /translation="ADFMKTDDKIDFLSKYGREAFTNVYYSALFNFKTQMAPGYNYPN
                     DSVKISNFLAPAYLLAAIGMDYKPNAYLSAFIAPATGRLIIVNDELLSNAGAFGVEPG
                     KTTKAEFGGYLRAIYTRNDFKTELLKNLSFTIKIDLFSDYLDHPQNIDINWETLVAFK
                     VNQYISVNFTSHLIYDDDTKISIDKDNDGVIDEFGPRVQFKEILGVGFSYKF"
     gene            655..1080
                     /gene="mscL"
                     /locus_tag="PLO58_09405"
     CDS             655..1080
                     /gene="mscL"
                     /locus_tag="PLO58_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQO75041.1"
                     /translation="MKVKAKPQSPKLLQEFKAFAMRGNVIDMAVGIIVGAAFGKIVTS
                     VVNDIIMPPIGLLVGGVNFTDLKIVLKHAVDGNPAVTLNYGNFLQVVFDFLIIAFAVF
                     MLVKALSAAKREEEAAPPAPPAPTKTEILLTEIRDLLKK"
     gene            1177..1431
                     /locus_tag="PLO58_09410"
     CDS             1177..1431
                     /locus_tag="PLO58_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004864811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQO75042.1"
                     /translation="MKCRKKCGACCIAISISSPLPGMPNGKPAGVRCIHLTVENYCKI
                     HDQPDYPAVCRNLKPSTEMCGESFDEAMKYLIKLEKLTSS"
     gene            complement(1388..>1605)
                     /locus_tag="PLO58_09415"
     CDS             complement(1388..>1605)
                     /locus_tag="PLO58_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO75043.1"
                     /translation="QLLTELRIVQVLGSTPLGLRSYWRFWTQDIILSYSGSTPAGLRG
                     VSHFYDSGYNPELFMKMSIFPILLNIS"
BASE COUNT          489 a          342 c          313 g          461 t
ORIGIN      
        1 gctgatttca tgaaaaccga tgataagatt gattttttat cgaaatatgg ccgagaagcc
       61 ttcactaatg tctattattc agcccttttt aatttcaaga cccagatggc accgggttac
      121 aattatccca atgattcggt aaaaatttcc aattttttag caccggctta ccttttagcc
      181 gcaatcggta tggattacaa accaaacgcc tatttgagtg cttttatcgc gcccgccacc
      241 ggacgattaa tcatcgtcaa tgatgaatta ctgtccaatg ccggagcttt tggagtcgag
      301 cccggaaaaa ctaccaaggc tgaattcggt ggttatctgc gtgcaattta tacacgcaat
      361 gattttaaaa ccgaattact aaaaaatttg tcttttacca ttaaaattga tctcttttcg
      421 gactatctgg atcatcctca gaatattgat attaactggg aaacgttggt tgcttttaag
      481 gtaaaccagt atatctcggt caatttcacc tctcacctga tttacgacga tgacacaaag
      541 atctcaattg ataaggataa tgacggagta attgacgaat tcgggccgcg ggtgcaattc
      601 aaagaaattc ttggcgttgg cttttcttac aaattctaac tatagaggat aaatatgaaa
      661 gtaaaagcaa aaccacaaag cccaaaacta ctgcaggagt ttaaagcttt cgcaatgcgc
      721 ggcaatgtta ttgatatggc agtcggtatc attgtcggtg ccgctttcgg caagatcgtc
      781 acttcggttg ttaatgacat tattatgcca ccgattggct tattggtcgg cggcgttaat
      841 tttaccgatt tgaaaatcgt attgaaacat gcggttgatg ggaatccggc ggtgacgcta
      901 aattatggca attttctgca ggttgtattt gatttcctca tcatcgcttt tgcggttttt
      961 atgttggtaa aagccctcag cgccgctaaa cgggaagaag aagccgcacc accagcgcct
     1021 cccgccccaa ctaaaacaga aatacttctg acggaaatcc gcgacttact gaaaaaataa
     1081 agtctaaccc ggtaccgcaa aaatatacta atagaaacta tttatttaca tctatctaaa
     1141 ccatccatag caattactta ttcttacgat tattaaatga aatgccgtaa aaaatgcggc
     1201 gcttgctgta ttgctatttc tatttcatcg ccacttcctg gtatgccaaa cggcaaaccg
     1261 gctggggtac ggtgtattca cttaaccgta gagaattatt gcaagattca cgatcagcca
     1321 gattatccag ctgtttgccg caatttgaaa ccttccactg aaatgtgcgg tgaatctttt
     1381 gacgaggcta tgaaatattt aataaaattg gaaaaattga catcttcata aataactcag
     1441 gattatatcc tgagtcatag aaatgcgaaa caccacgcaa ccccgcaggg gttgaacctg
     1501 aataactcag gattatatct tgagtccaaa aacgccaata actacgcaac cccaacgggg
     1561 ttgaacctaa gacctgaaca atccgtaact ccgtcaggag ttgaa
//
LOCUS       DAOMJU010000158         1602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_97422_length_1602_cov_23.809308, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000158 DAOMJU010000000
VERSION     DAOMJU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97422_length_1602_cov_23.809308"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..481)
                     /locus_tag="PLO58_09420"
     CDS             complement(86..481)
                     /locus_tag="PLO58_09420"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO75044.1"
                     /translation="MRKIILILGGTICVVLVGLGLIGCEGDEANQPSYILDIDPIFGS
                     AKYNCKGCHTGATSPYLDLTTYEGLMAGSDNGKVVIPGDADHSFLYEKISQPIPSRGE
                     RMPLGGPFYLTSGEIRLIEDWINLGAKDN"
     gene            complement(493..1404)
                     /locus_tag="PLO58_09425"
     CDS             complement(493..1404)
                     /locus_tag="PLO58_09425"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQO75045.1"
                     /translation="MSRKFRLILLGMALVSAASAQALYQGLYQPIDARGWGMGSAMVA
                     QTRNSSGVAYNPAILAVVPNKWQVNYTRYVLDIQSTNVLFVWQAPGKGKLAALGGYLD
                     YGSFSESDDNGNELGEFHVSDLNLRLAYGVPITRKLSAGLAAIFVNSDLANYNSKALL
                     GSAGLLYYDPVSTFSAGLAHTNFGKMLSGYINDDEKIHSALMAGIAKKLEHLPMVIAA
                     DFMHYSSGENIIKIGGEFILGQNYFLRWGTSSRRFEINTQQTITNFFSSSSLGGGLVI
                     NRLQIDLTWYSLGHTGSVFALSIAQNL"
     gene            complement(1388..>1602)
                     /locus_tag="PLO58_09430"
     CDS             complement(1388..>1602)
                     /locus_tag="PLO58_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQO75046.1"
                     /translation="CEYDDLMNQWQTYVEKKYNWIFLLDIQNILWLIIPLIAFLAYWS
                     IRRRNKVTLEQWNDEEINENSDEQEI"
BASE COUNT          442 a          398 c          321 g          441 t
ORIGIN      
        1 ttcttataaa aatttcgttc aggatagacc taccgttata atcccattag taaatgtttt
       61 attcggctaa tctatctaac tcggcttaat tatccttagc gcccaggttg atccaatctt
      121 cgattaacct gatttccccc gaggttaaat aaaaaggacc gccgagcggc atacgttcgc
      181 ctctggaagg gattggctgg gaaatttttt catataaaaa actatggtca gcgtcacccg
      241 gtatcaccac cttgccatta tcactgccgg ccattaaacc ttcgtaggtc gtcagatcaa
      301 ggtatggcga agtagcgccg gtgtggcatc ctttacagtt atatttagca cttccgaaaa
      361 taggatcaat atctaaaatg tagctgggtt gattggcttc atcaccttca catccgatta
      421 gtccaagccc cactaagacg acgcatattg tcccgcctaa aattagtatt atttttcgca
      481 taatgcacct ttctataggt tctgggcaat gctgagggca aaaacactgc cggtgtgtcc
      541 gagactgtac caggtcaggt cgatctgtaa acggttaatt accaagccgc cgcctaacga
      601 agaagaactg aaaaaattag tgatggtctg ctgggtgttg atttcaaaac gccggctcga
      661 tgtcccccag cgcaagaaat aattttgtcc gagaatgaat tctccgccga tcttgatgat
      721 attttctcca gatgaataat gcataaagtc agccgcgatc accattggca aatgttcaag
      781 cttcttggca atgccagcca ttagcgcgga atgaattttc tcatcatcgt tgatatatcc
      841 tgaaagcatt ttcccaaaat tcgtatgagc cagtccagct gaaaacgtcg agaccggatc
      901 ataatacaat aaacccgctg aacctaataa cgcctttgaa ttgtaatttg ctaagtcgga
      961 attcacgaag attgccgcca gcccggcgct gagtttgcgg gtgattggga ctccataagc
     1021 tagtcgcaga tttaaatcgc tgacgtgaaa ttcgccgagt tcatttccat tatcatcact
     1081 ctcgctaaaa ctgccgtagt ccagatagcc gcccaatgcc gccaatttac cttttcccgg
     1141 cgcctgccag acaaatagaa cgttggttga ttgaatgtcc aatacgtagc gggtataatt
     1201 cacctgccat ttgttcggaa caaccgccag aatcgccgga ttatatgcca cgccggacga
     1261 attgcgcgtc tgggcaacca ttgctgaacc cataccccag ccgcgggcat caatcggctg
     1321 ataaagtccc tgataaaggg cttgcgccga cgcggcgctc actaatgcca ttccgagtaa
     1381 tattaaccta aatttcctgc tcatcgctat tttcattgat ttcttcatca ttccactgct
     1441 cgagagtcac tttgttccgc cgccgaatac tccaataagc caaaaacgct atcaacggta
     1501 taattaacca caaaatattc tgaatgtcca gaagaaatat ccaattgtac tttttttcaa
     1561 cataagtttg ccactggttc atcaaatcgt catattcgca at
//
LOCUS       DAOMJU010000159         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_97517_length_1601_cov_22.773609, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000159 DAOMJU010000000
VERSION     DAOMJU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97517_length_1601_cov_22.773609"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1272)
                     /gene="rho"
                     /locus_tag="PLO58_09435"
     CDS             complement(13..1272)
                     /gene="rho"
                     /locus_tag="PLO58_09435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQO75047.1"
                     /translation="MENELTIAKLQSMKIKELSDIANQLEIPETTGLKKMDLIYKILE
                     ASAQKEGKIFAQGVLQVMDEGYGFLRSEKSSYLPGSDDIYISPSQIKRFGLRTGHIVC
                     GQIRPPKDNERFFALLRVDAVNMMDPEEVKAHPVFDSLTPLYPQSRIQLETDSKNISM
                     RIMDLLCPIGKGQRGLIVAQPKTGKTTLLQQIANSITTNHPEITLMVLLIDERPEEVT
                     DMERSVKAEVISSTFDEPPERHVQVADMVLEKAKRMVELGFHVVILLDSITRLGRAHN
                     AVVPHSGKILSGGIDANALHRPKRFFGSARNIEGGGSLTIIATALIDTGSRMDDVIFE
                     EFKGTGNMELVLDRRLSDRRIFPSMDVNRSGTRKEELLLSAAELARVWILRKLLSEMT
                     PIEAMEFLIDRMNRTKSNREFLKSMNQ"
BASE COUNT          460 a          336 c          320 g          485 t
ORIGIN      
        1 tataatagcc gtttactggt tcatagattt gagaaattca cgattgctct tggtgcgatt
       61 catacgatca atcaggaatt ccatggcttc gattggagtc atttcgctta ataatttccg
      121 caaaatccag actcgtgcca gttccgccgc tgaaagtaag agttcctctt tgcgcgtacc
      181 ggaacgattg acatccattg aagggaaaat acggcgatcg cttaaccggc ggtccagtac
      241 taattccata ttaccggtgc ccttaaactc ttcaaaaatc acgtcatcca tacgggaacc
      301 ggtgtcaatc agagccgtgg cgataattgt caggctaccg ccgccttcga tatttcgggc
      361 agaaccaaaa aagcgcttgg ggcggtgtag agcattggcg tcaataccac cggataggat
      421 tttaccactg tggggaacga cggcattatg agctcgccca agacgggtaa tactatccag
      481 caaaatgaca acatgaaatc ccagttcgac catacgcttg gctttttcta agaccatatc
      541 tgccacttga acatgacgtt ccggtggttc atcaaaagtc gaactaatga cctcggcttt
      601 gacggaacgt tccatatcag tgacttcctc gggacgttcg tcaattagca ataccatcaa
      661 cgtaatttca gggtgattgg tagtaatact attggctatt tgctgaagca aagtcgtctt
      721 cccggttttt ggttgagcga caatcagtcc tctctggcct ttgccaattg gacaaagcaa
      781 atccataatg cgcatcgaga tgttttttga atcggtttcg agctgaattc ggctttgagg
      841 atataaaggc gtcagggaat caaaaaccgg gtgcgctttt acctcttccg gatccatcat
      901 attaaccgca tcaacgcgaa gaagtgcgaa aaaacgttcg ttgtctttag gaggccgtat
      961 ctgtccacaa acaatatgac cggttcgcaa accaaatcgt ttgatttgag aaggtgatat
     1021 gtatatatca tcagaaccag gtaaataact cgatttctca gagcgtaaaa atccatagcc
     1081 ttcatccatc acctgtagaa caccctgggc aaatatttta ccttcttttt gagcactggc
     1141 ttcaagaatc ttataaatca aatccatttt tttaaggcca gttgtttctg gaatctccag
     1201 ctgattagct atatcggaca attctttgat tttcatcgat tgcagttttg caatggtcaa
     1261 ctcattttcc atagttattc cttttttaaa ggttttacac gaatcgttag tctaaataga
     1321 ggtaggtcaa aaaattaatt gattattaat taatgtcttt aatttaaata gtcaaattca
     1381 taagtcaaat ataatttttc tcagcagtat attaaatgga cattaggtca gaagatcgcg
     1441 tttactcgta atcagtaatt tttcactctt aaattcagct ttttttgcta aagctaagat
     1501 tgggactaag caaggagact tgactgtagt atcaaaaaac ctgaatcatt cttaaaactt
     1561 tcttagcagg cttgagtcga gttgaatttt atgaattctc c
//
LOCUS       DAOMJU010000160         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_100970_length_1566_cov_18.050298, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000160 DAOMJU010000000
VERSION     DAOMJU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100970_length_1566_cov_18.050298"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..1556)
                     /gene="tviB"
                     /locus_tag="PLO58_09440"
     CDS             complement(276..1556)
                     /gene="tviB"
                     /locus_tag="PLO58_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012140193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HQO75048.1"
                     /translation="MSIIGLGYVGLPLAVEFAKNFPVTGFDIKKERVEELRNGHDATL
                     EVEDDHLKSVLINQNGRTGLFLTDQLDDIRPCNYYIVTVPTPIDQYKRPDLTPVIKAT
                     ETVSRILKKGDIVIYESTVYPGCTEEVCVPILEKGSGLHFNTDFFCGFSPERINPGDK
                     VHTVTKIKKVTSGSTPEIARKIDALYQTIITAGTFLASSIKVAEAAKVIENTQRDLNI
                     AFVNELAKLFNMMSIDTKEVLEAAGTKWNFLPFQPGLVGGHCIGVDPYYLTFKAQGIG
                     YHPEVILAGRRINDSMGFYVAEQVVKLMIKKGHTINASQVLILGITFKENCPDVRNSR
                     VIDIITELQQYGCRVDVYDPWADSQEVKREYGIDLVAQFSQKMGNKQLPHYDAIVLAV
                     AHNEFKTLNLKPLKNNRTVIYDVKGILAEYDGRL"
BASE COUNT          428 a          324 c          329 g          485 t
ORIGIN      
        1 tttctcacgg ctaaattaag ccgactcaaa tcatcagtga gttattgtca ggaacagctt
       61 ttcagggcat tcgtttcagg cattttgagt gacatacttt aataattctt tcccatttcc
      121 tgtgacaagc taattgataa tttaaattaa taaatcatag ttaggatgtc aatcaaattt
      181 taatccttta agatcgaacc aatgtttagt tgagattgat gtgcgctgaa tcacttttta
      241 cctactcttg cgattatctc agaaaaagta tcttgctata accttccgtc gtattccgcc
      301 aggattcctt tgacatcata gataacggtt cgattatttt tcaatggctt taagttcaat
      361 gttttaaatt cattgtgagc aactgccaat acgatagcat cataatgggg aagttgctta
      421 ttacccattt tttggctgaa ttgagcgact aaatctattc cgtattcacg tttgacttcc
      481 tgggaatccg cccagggatc gtaaacatca acccgacatc catattgttg aagttcagta
      541 ataatgtcaa tgactcgaga attgcgcaca tcggggcaat tttccttaaa ggtgataccc
      601 agaatcaata cctgcgaggc attaattgtg tgcccttttt tgatcatcag tttgacgact
      661 tgttcagcca cataaaaacc cattgaatcg ttgattcttc gtcccgctaa aatgacttcc
      721 ggatgatagc ctatgccctg agctttaaat gttaaataat atggatcaac gccgatgcaa
      781 tgcccaccaa ccaaaccggg ttggaaaggc agaaaattcc atttagtccc agccgcttct
      841 aatacttctt tagtatcaat gctcatcata ttgaaaagct tggcgagttc gttgacaaac
      901 gcaatgttga ggtcgcgctg agtattctca atcactttag cggcttcggc aacttttata
      961 gacgaagcca ggaaagttcc ggcagtgatg atggtttggt aaagagcatc aattttgcga
     1021 gcaatttcgg gtgtggaacc ggaagtaact tttttaattt tcgttacggt atgaactttg
     1081 tcgccgggat taatgcgttc cggagagaag ccgcagaaaa agtcggtatt gaaatgcaag
     1141 ccactgcctt tctcgagaat tggtacgcaa acctcttcag tgcaacccgg gtaaaccgtg
     1201 gattcataaa tgacaatatc gccttttttt agtatgcgac taactgtttc ggtggctttg
     1261 atgaccggtg ttaaatcagg acgcttgtat tgatcaatgg gagtaggcac cgtgacgatg
     1321 taataattgc agggtcgaat atcgtcgagt tgatcggtta agaacagacc agttcgcccg
     1381 ttttggttaa taaggaccga cttcaggtgg tcgtcttcaa cttccagggt ggcatcgtgg
     1441 ccatttcgta attcttccac tcgttctttc ttaatatcga atccggttac gggaaaattt
     1501 ttagcaaact cgactgccag cggcaagccg acgtaaccaa gtccaatgat gctgattctg
     1561 tcggta
//
LOCUS       DAOMJU010000161         1550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_102559_length_1550_cov_18.274916, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000161 DAOMJU010000000
VERSION     DAOMJU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102559_length_1550_cov_18.274916"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLO58_09445"
     CDS             complement(<1..883)
                     /locus_tag="PLO58_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HQO75049.1"
                     /translation="MFGKGIRRLFFGLSVLLVAMAYGDSNIVINPGFEDGKEGWFDRT
                     CAIEIVTSPVYEGRYSGKAFQRLANWQGIKQSVFGRMVDGKTYQVSGWVRLDNAPSDE
                     VAISFEQQDDSGTKYIGVARGIATDTAWTKLSGEFTLNVTGTLSVLDVYFEGPAPGVN
                     FYVDDVVVYGPEANPPVIIPPTPQSKSQVDAKIRHQIIEGFGASGAYYTMNLVNHNKK
                     TELYNLLFKELRLDIFRIRNNYDMEPNTMQETVEIIKNAKAVRGDNLKILMSSWSPPA
                     YLKSNNNVVGGTLKKVAG"
     gene            complement(1100..1173)
                     /locus_tag="PLO58_09450"
     tRNA            complement(1100..1173)
                     /locus_tag="PLO58_09450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1137..1139),aa:Arg,seq:tct)
     gene            complement(1197..1272)
                     /locus_tag="PLO58_09455"
     tRNA            complement(1197..1272)
                     /locus_tag="PLO58_09455"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1234..1236),aa:Pro,seq:tgg)
BASE COUNT          451 a          360 c          294 g          445 t
ORIGIN      
        1 ttccggcgac ctttttgagc gttccaccta ccacattgtt attactcttc aaataagctg
       61 gtggggacca cgaagacatc aaaattttca aattgtctcc ccgtaccgcc ttagcgtttt
      121 tgataatctc gacggtttct tgcatcgtat tcggttccat atcataatta ttgcgaatgc
      181 ggaatatgtc cagtctgagt tctttgaaaa gcaggttata cagttctgtc tttttattat
      241 gatttaccag attcattgta taataagctc cagaagcacc gaagccctct attatttggt
      301 gacggatttt ggcatccacc tggcttttgc tttgcggcgt gggtggaatg attaccggtg
      361 gattcgcctc cggaccatat acgactacat catccacgta gaaattgacg cccggcgccg
      421 gtccttcgaa atacacatcc agaaccgaca aagtaccagt cacatttaat gtaaattcac
      481 cggaaagttt agtccaggca gtatcggtgg ctatcccgcg agcgaccccg atgtattttg
      541 ttccgctgtc gtcttgttgt tcaaaagaaa tggcgacttc gtcgctgggc gcattatcta
      601 atctgaccca gccggaaact tgataggttt tgccgtcaac catcctgcca aataccgact
      661 gcttgatacc ctgccagtta gctaatcgct gaaatgcttt tccgctatag cgaccttcat
      721 ataccggcga ggttacaatt tctatggcgc aagtgcgatc aaaccaaccc tcttttccat
      781 cttcaaatcc gggattaatt acaatgttac tatccccata agccattgct accagcagaa
      841 cactcaatcc gaaaaataat ctgcgaattc ctttcccaaa cattttgcta atactcctta
      901 gttagaattt cataatgaac aaaaatctac aatttattgc caattttaaa aacttatttt
      961 cagtaatcaa aactaaccgg ataaaaatct ccgagtagtc gggattcctg cggaagaacc
     1021 tgtaatcacc tgcttagaag ttcaatgctc tatcatttga aatatttaac gttaataatg
     1081 aataagtaaa atatgcgcgt accccgagca ggactcgaac ctgcaaccac ctgcttagaa
     1141 ggcagatgct ctatccactt gagctatcgg ggcattagtc aaaatcagaa agatcgtcgg
     1201 ggtgagtgga tttgaaccac cggccccctg ctcccaaagc aggtgcgcta aaccggactg
     1261 cgctacaccc cgttctccaa attctatctc tgattatgaa aaaacaatca tttcaagtta
     1321 ttataataaa ctcgtttatc agtggtaaaa agcacgtaaa gttaggatat taaagccatt
     1381 aaagtcaagc cgaaaatatt gtttgccttt tagctaaata tagtctatat ttagctatgt
     1441 ggctaataat aaaaatagcg attaatgagg agtgttaaaa agaaatgaat acggtctatc
     1501 gggcgctatc cgatccaacc cggcgacaaa ttctgaaatt actgcgcgag
//
LOCUS       DAOMJU010000162         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_106143_length_1517_cov_19.067031, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000162 DAOMJU010000000
VERSION     DAOMJU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106143_length_1517_cov_19.067031"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..590)
                     /locus_tag="PLO58_09460"
     CDS             complement(111..590)
                     /locus_tag="PLO58_09460"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HQO75050.1"
                     /translation="MINEIIKMIAITFVPTLELRASIPYGIFAAKISWLIVFAVCVVA
                     NIIVGILIYLLMDLIIKIITKIPVITKVWDNYVAKTQQKIQKGVDKYGKWALAIFIGI
                     PLPGSGVYTGALAAYLIGLRFKKFMVANVIGVIIAGIAVTVICLTGASVSNIFIKQM"
     gene            complement(668..1348)
                     /locus_tag="PLO58_09465"
     CDS             complement(668..1348)
                     /locus_tag="PLO58_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQO75051.1"
                     /translation="MNNRNYCIIIPAYQAAETLPSLLKIITMQFAENDIFIVDDGSTD
                     QTSDVINDYPQIHLLKQPVNRGKGAALHSGLRAAYRLGYQFAVCLDADLQHDPNYISR
                     FIAAQKQTGSDLVLGVRDFAFRAMPFHRILSNTITSFLISIRTGRRIHDSQCGYRLLN
                     LKIVQPFQFSENGFQFESEVPIKLLLAGFKYTEVKIPTIYRANHSSINNIKDTYKFIR
                     LFFKSYLW"
     gene            1442..1514
                     /locus_tag="PLO58_09470"
     tRNA            1442..1514
                     /locus_tag="PLO58_09470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1475..1477,aa:Gly,seq:gcc)
BASE COUNT          490 a          286 c          293 g          448 t
ORIGIN      
        1 cctgcccgat taactgatgc caatcggtca ggcgggcgct acgccttaag ataattgtca
       61 aaaaagcggt taaatttatt attatatatt agaatattca aacagaaagg ctacatctgc
      121 ttgataaaaa tattactaac actggcaccc gttagacaaa taaccgttac tgctattccg
      181 gcaatgataa cgccgataac attggctacc ataaatttct tgaaccttaa gccaatcaaa
      241 taagccgcca gcgctccggt ataaacacct gaaccaggta gaggaattcc gataaatatt
      301 gccagagccc atttaccata tttatcaacc cctttttgaa ttttttgctg ggtttttgcc
      361 acataattat cccagacttt ggtaataacg ggaattttcg taataatttt aattatcaag
      421 tccattaata agtagattag aataccaaca attatattag caaccacaca aaccgcgaat
      481 acaattaacc acgaaatttt agccgcaaaa atgccgtatg gaatcgaagc tcgtaattcg
      541 agtgtgggga cgaaagttat cgcgatcatt tttataattt catttatcat tcattttcca
      601 aatagtaatc ggcggctaaa ttacaataat ttgactgttc cggaaatagc cgaattataa
      661 aaattttcta ccataaataa ctcttaaaaa ataggcgtat aaatttatag gtatctttta
      721 tattgttaat tgaactatga ttggcacggt atatcgtcgg gattttaacc tcggtatatt
      781 tgaaaccggc taagagtaac ttgattggaa cttcgctttc gaattgaaaa ccgttttccg
      841 aaaattgaaa tggttgcaca attttaagat taagcaaacg atatccacat tggctatcat
      901 gaatgcgtcg tccagttcgg atggaaatta aaaaggaagt gatggtattg gatagaattc
      961 gatgaaacgg catcgctcgg aaggcgaaat ctcggacgcc cagaactaaa tcggagcctg
     1021 tttgcttttg agctgcaatg aaacgactaa tataattagg atcgtgttgt aaatcagcgt
     1081 ccagacacac tgcaaattgg taaccaagtc ggtaagctgc gcgcaaacct gaatggagtg
     1141 cggctccttt acctcgatta acaggctgtt taagtaaatg aatttgcgga taatcgttaa
     1201 tgacgtcact cgtttgatct gtcgaaccgt catctacaat aaaaatatca ttttcagcga
     1261 attgcatagt gataattttc agcagagacg ggagagtttc tgccgcttgg taagccggaa
     1321 taataatgca atagttacga ttattcatac tgaaaattag attatttagc agataccgtc
     1381 aaatgtaaaa attattgcat aaagtaggcg aagagtttaa atttacgcgc tttttgttta
     1441 tgcgagagta gctcagctgg tagagctcca cgttgccaac gtggctgtcg cgggttcaag
     1501 tcccgtctct cgctcaa
//
LOCUS       DAOMJU010000163         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_4890
            NODE_107619_length_1504_cov_17.295376, whole genome shotgun
            sequence.
ACCESSION   DAOMJU010000163 DAOMJU010000000
VERSION     DAOMJU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867049
            Sequence Read Archive: SRR5168925
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 19:03:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,857
            Genes (coding)                    :: 1,850
            CDSs (with protein)               :: 1,850
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107619_length_1504_cov_17.295376"
                     /isolate="AMR_MDS_4890"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLO58_09475"
     CDS             complement(<1..527)
                     /locus_tag="PLO58_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HQO75052.1"
                     /translation="MNFRDRKYWVGFAKALAIIMLIYLFLLSVTLIGDTFKMMGEGFA
                     QKIMKGVSNPFVGLFAGMLCTAIVQSSSMTTSLVVSMVAGNVLTINLAIPIIMGANIG
                     TTITNVLVALGHIGKKDEFERAFAVATMHDFFNILSVIIIFPFQYFFNILGIAAGAVA
                     TAFENIGALKFINPI"
     gene            847..>1504
                     /locus_tag="PLO58_09480"
     CDS             847..>1504
                     /locus_tag="PLO58_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQO75053.1"
                     /translation="MKSSNIFDVILIGAGPAGLFAAETLAGKSLSVLVLDRGDEPLKR
                     HDVNFGIGGAGAFSDGKLNLTSRIGGEPESFGRKADEVEELIRQIDDIFTSLGVIGGY
                     SGEDGEAYQALLRSAHAAGVEFIFAKQRHIGTDRLQDIIQRFYERLIEKGINFRTNTY
                     VDKISKEGDYFKLICKDEVLFARAVIAAPGRAGAYWLREQAHNLGIHTMYGPIDVGVR
                     V"
BASE COUNT          439 a          285 c          320 g          460 t
ORIGIN      
        1 ctaattggat tgataaactt caaagcgccg atattttcaa aagcagttgc cacagcaccg
       61 gcggcaattc cgagaatatt gaagaaatat tgaaatggga atatgatgat gacgcttaag
      121 atattgaaaa aatcgtgcat cgtagcgacc gcaaatgctc gctcgaactc atcctttttc
      181 ccaatatgtc ccagtgccac taaaacattg gtgatggttg tgccgatatt cgcgcccata
      241 atgattggaa ttgccaaatt gattgtcaga acattaccgg ctaccatact cactactaac
      301 gaagtcgtca ttgaggaact ttgcacaata gccgtgcaaa gcatcccagc aaaaagaccc
      361 acgaacggat tagatacgcc tttcataatt ttttgggcaa aaccctcacc catcatttta
      421 aaggtatcgc caatcagggt gacacttaag agaaaaagat aaatcagcat tatgattgcc
      481 agcgccttgg caaatccaac ccaatatttc ctatccctaa aattcattcc agtcctttta
      541 tatgattaaa taatttcaaa aatcgggcgg aatttagaaa aaaatcggct aataaccgca
      601 ttttttggtt agtctcttct taattatttt tacttgtttt taagttatat gttaaatttt
      661 aataatgagt tgaagtaata ctcctaatcc acccatccgt taatctttct caaatgaact
      721 taaaatcact taatcgcact ttaacgtgca ttattagtgc gtgttttgta caaaaatctt
      781 tcctaacttt ggatgttttt tggatatttt tgtaaaaata accactttat ttataaaggg
      841 atcggtatga aatcgtccaa catatttgat gtcatattaa tcggtgctgg accggcaggt
      901 ttatttgcgg ctgaaaccct ggctggcaaa agtttatcag tgctggtttt agatcgcggt
      961 gatgaaccgt tgaaacgtca tgatgtgaat ttcggaattg gtggcgccgg tgctttttcg
     1021 gacggcaaat tgaatttgac ttctcggatt ggtggcgaac cggaaagttt tggtcgcaaa
     1081 gctgatgaag tggaggaatt gattcggcag atagatgata tttttacttc acttggagta
     1141 atcggtggtt attcaggaga agatggcgag gcttatcagg ctttattgcg gtctgcccac
     1201 gccgccgggg tagagtttat atttgccaaa cagcgtcaca ttggaaccga ccgcctgcag
     1261 gatattatcc aacgctttta tgaacgatta attgaaaaag gtataaattt tcgcactaat
     1321 acctatgttg ataaaatttc taaagagggc gattatttta aattaatctg caaggatgag
     1381 gttctttttg ccagagcagt gattgcggct cccggcagag cgggcgctta ttggttgcgc
     1441 gaacaagctc ataatctcgg gattcacacg atgtatggac cgattgatgt tggagtgcgg
     1501 gttg
//
